LOCUS       DBJGGI010000001        94896 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.001, whole genome shotgun sequence.
ACCESSION   DBJGGI010000001 DBJGGI010000000
VERSION     DBJGGI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 94896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94896
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.001"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(<1..454)
                     /locus_tag="ACSIGJ_000001"
     CDS             complement(<1..454)
                     /locus_tag="ACSIGJ_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HHQ1329016.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWYYVRESGADGILKATYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGVHEWHK"
     gene            706..3168
                     /gene="lon"
                     /locus_tag="ACSIGJ_000002"
     CDS             706..3168
                     /gene="lon"
                     /locus_tag="ACSIGJ_000002"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HHQ1329017.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAKLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMTKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLGLPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVRNPLFLLDEIDKLGSDFRGDPASALLEVLDHE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELVVEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLNE
                     DKKRLSETKKTSKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGMIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNVKDLEEIPENVKTGLTIHPVKWIDEVLALGLESRPESWAEP
                     SAAEAAAESASKPKPRSRAIKH"
     gene            3350..3619
                     /locus_tag="ACSIGJ_000003"
     CDS             3350..3619
                     /locus_tag="ACSIGJ_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HHQ1329018.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTNALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTLKFRAGKALKDAL"
     gene            complement(3795..4229)
                     /locus_tag="ACSIGJ_000004"
     CDS             complement(3795..4229)
                     /locus_tag="ACSIGJ_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329019.1"
                     /translation="MTEPKHETPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALL
                     SQCAMSKPQAKQKIVESCMKNIPFAEKWQNDLKARGLDADNTRLAVDYCKCMWEQPLD
                     GLSEKQISSFGKLGAQEQLDLLGGANAFETRDKQCVADLKAD"
     gene            complement(4222..5529)
                     /locus_tag="ACSIGJ_000005"
     CDS             complement(4222..5529)
                     /locus_tag="ACSIGJ_000005"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HHQ1329020.1"
                     /translation="MKPVNIGLLGLGTVGGGAAAVLRDNAEEISRRLGREIRISAMCD
                     LSEEKARQICPSAAFVKDPFELVARKDVDVVVELFGGTGIAKEAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     DGVMNAVRVNANMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            5725..6096
                     /locus_tag="ACSIGJ_000006"
     CDS             5725..6096
                     /locus_tag="ACSIGJ_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HHQ1329021.1"
                     /translation="MLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVHKDSVCLPQ
                     CRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGISVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            6173..8095
                     /locus_tag="ACSIGJ_000007"
     CDS             6173..8095
                     /locus_tag="ACSIGJ_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HHQ1329022.1"
                     /translation="MIEIKNLTLQRGLKVLLDKASAAVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDISIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     VQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYSGNYDFYQTERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYNPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVHSEKLNIGYFAQHQLDTIRADQ
                     SPVWHIQQLSPEVREQEIRNFLGGFDFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIVVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAATAPAASTQSQNRKDTKRIEAQIRQEKARRSKPIQQKIDKA
                     EKEMVQLSEIQTGCEAFLTQEDAYLEANKEKLQNTLSELAKVKTQLAQIEETWLACQE
                     ELERIETEIEKQFAER"
     gene            8082..8477
                     /locus_tag="ACSIGJ_000008"
     CDS             8082..8477
                     /locus_tag="ACSIGJ_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HHQ1329023.1"
                     /translation="MPSDKEARAGCLSLPANNGIIRTLPPPFYFTGINIRLFAALGFL
                     SLSGAAAQASIYHCNSNGKSVYTSDPSGSCADADLPKISSHQGGGYRLKIKKLSQEAK
                     IHTGNKKKNKKHAGKKNRQAAKAPKENQK"
     gene            8568..9080
                     /locus_tag="ACSIGJ_000009"
     CDS             8568..9080
                     /locus_tag="ACSIGJ_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329024.1"
                     /translation="MKSKLPLILINLSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPAPSPSNGGQAVKYKAPVKTVSKPAKSNTPPQQA
                     PVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQKINALQSNVLDRQQNI
                     QALQRELGRM"
     gene            complement(9441..9623)
                     /locus_tag="ACSIGJ_000010"
     CDS             complement(9441..9623)
                     /locus_tag="ACSIGJ_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329025.1"
                     /translation="MGVCPDARCSAAGIARQIGNGGGIHGTLAFSLLFLSLGHIGMVA
                     YHHIKGESVLPLFLLI"
     gene            9623..9958
                     /locus_tag="ACSIGJ_000011"
     CDS             9623..9958
                     /locus_tag="ACSIGJ_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329026.1"
                     /translation="MPNLVTAVLAGRFLRWRIPYPTHQTDNISSRILTARICWYESSA
                     QAEKAVAPKTVARINCLNFMMLLLLHKAFGIVKPAAYGFAAVAALQAVLQETLKHDHC
                     IKSCSNSAK"
     gene            complement(9995..10654)
                     /gene="ung"
                     /locus_tag="ACSIGJ_000012"
     CDS             complement(9995..10654)
                     /gene="ung"
                     /locus_tag="ACSIGJ_000012"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HHQ1329027.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPEADVFNA
                     FRLTAFDRVKVVILGQDPYHGVGQAHGLAFSVRQGVRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGILLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSQHGIEPINWK
                     L"
     gene            10735..11142
                     /locus_tag="ACSIGJ_000013"
     CDS             10735..11142
                     /locus_tag="ACSIGJ_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HHQ1329028.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDTGKYAEILRYTGGNRYEVFYRGT
                     HWQAQNTGQEVFEPGTRALIVRKEGNLLIIANP"
     gene            11157..12104
                     /locus_tag="ACSIGJ_000014"
     CDS             11157..12104
                     /locus_tag="ACSIGJ_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HHQ1329029.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRAMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(12211..13281)
                     /locus_tag="ACSIGJ_000015"
     CDS             complement(12211..13281)
                     /locus_tag="ACSIGJ_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HHQ1329030.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVYALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FIQNTLLPWLKNTIGGYVEIDQASIIAWFQAHTGELSNALKAWFPVLMKQGGNIVSSI
                     GNLLLPPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLMLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LAVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGELMGFVGMLAGLPLAA
                     VTLVLLREGAQKYFAGSFYRGR"
     gene            13466..13741
                     /locus_tag="ACSIGJ_000016"
     CDS             13466..13741
                     /locus_tag="ACSIGJ_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HP0495 family protein"
                     /protein_id="HHQ1329031.1"
                     /translation="MTEQKNKASLIEFPCAFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            13743..14366
                     /gene="lipB"
                     /locus_tag="ACSIGJ_000017"
     CDS             13743..14366
                     /gene="lipB"
                     /locus_tag="ACSIGJ_000017"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HHQ1329032.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGIYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTQ
                     INPCGYAGMEMTQIADFVQPCPAPDEVASKLTAHLETQLTPKADNNE"
     gene            14359..15342
                     /gene="lipA"
                     /locus_tag="ACSIGJ_000018"
     CDS             14359..15342
                     /gene="lipA"
                     /locus_tag="ACSIGJ_000018"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HHQ1329033.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFTNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            15817..16281
                     /gene="bfr"
                     /locus_tag="ACSIGJ_000019"
     CDS             15817..16281
                     /gene="bfr"
                     /locus_tag="ACSIGJ_000019"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HHQ1329034.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            16309..16782
                     /gene="bfr"
                     /locus_tag="ACSIGJ_000020"
     CDS             16309..16782
                     /gene="bfr"
                     /locus_tag="ACSIGJ_000020"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HHQ1329035.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELAKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            17110..17430
                     /locus_tag="ACSIGJ_000021"
     CDS             17110..17430
                     /locus_tag="ACSIGJ_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHQ1329036.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQVPS
                     ESGI"
     gene            17479..20037
                     /gene="glnD"
                     /locus_tag="ACSIGJ_000022"
     CDS             17479..20037
                     /gene="glnD"
                     /locus_tag="ACSIGJ_000022"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HHQ1329037.1"
                     /translation="MPENLSSALETFKQQRNAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEHFQNSALCLMAVGGFGRGEPYPCSDVDLAVVSPAPLSDGIQEQIARFIQTLW
                     DCKLMPSVKSGSVDELCESVRDDITGDTAFLEARFLFGNRQTADELAEKMNVQRNVAA
                     FIEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLAANLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIRLHLNAKRAEDRLLFDLQPQVAESMGYQDENRRR
                     QSEELMRVFYRAVKTVKQLGGILTPMLRSRVSSTPMRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRRRFAGF
                     FRSGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILAVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAVQGIADARQFAADHF
                     LTEEESDLLAWLVENHLLMSAVAQKEDIQDPGVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRCLAGNDGNPHALFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFEPPIVRSRILPQSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETQSCNRRISRRSRYMPIAPSITITPEEDYPDRYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERVEDSFTVFSPDLKNPKIQSSLKQALLEQLA"
     gene            20184..21647
                     /gene="guaB"
                     /locus_tag="ACSIGJ_000023"
     CDS             20184..21647
                     /gene="guaB"
                     /locus_tag="ACSIGJ_000023"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HHQ1329038.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTDERVKALVEAGADVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVTVGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(21983..24358)
                     /gene="rnr"
                     /locus_tag="ACSIGJ_000024"
     CDS             complement(21983..24358)
                     /gene="rnr"
                     /locus_tag="ACSIGJ_000024"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HHQ1329039.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLMPMDEGDFVLYERQMRGVMHGDTVTVRPAGIDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGKIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHRFSEACAKSAKKIPDHVRKSDLKGRVDL
                     CDLPLVTIDGETARDFDDAVFAEKVGRNYRLVVAIADVSHYVRPDDAIDADAQERSTS
                     VYFPRRMIPMLPENLSNGICSLNPDVERLCMVCDMVVTYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSDGIGNPHKAQIDTLYKLFKILQKKRLARGAVEFESVETQMLFDGNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTSEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALAEQFKGRPDAELLQVMMLRSMQQAVYEPHCEGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNRKTYTPNKSWQALGVHTSFCERRADDAGRD
                     VENWLKTYYMRDKVGEIFEGKISGVANFGIFVTLDDIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAGGSGRRRKVKLSASAKPAG
                     AAGKGKSKTTAEKKTARCGKVRGRGVPAVAESGKKAKKPVPIKVKKRKGKS"
     gene            24447..24533
                     /locus_tag="ACSIGJ_000025"
     tRNA            24447..24533
                     /locus_tag="ACSIGJ_000025"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24481..24483,aa:Leu,seq:cag)
     gene            24572..24662
                     /locus_tag="ACSIGJ_000026"
     tRNA            24572..24662
                     /locus_tag="ACSIGJ_000026"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24606..24608,aa:Ser,seq:gga)
     gene            complement(25059..26870)
                     /gene="typA"
                     /locus_tag="ACSIGJ_000027"
     CDS             complement(25059..26870)
                     /gene="typA"
                     /locus_tag="ACSIGJ_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HHQ1329040.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGCHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESSDMRPLFDTILKYTPAPSGSADEPLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHEQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLRVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(26987..27877)
                     /locus_tag="ACSIGJ_000028"
     CDS             complement(26987..27877)
                     /locus_tag="ACSIGJ_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HHQ1329041.1"
                     /translation="MFPVFHLSGESRRRMLQTALRFPHVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVRAGFNQDTLPFAVIAGFPEIMLDTADGLTKRQG
                     INAWTAGCGLGTDAAAVETVAAVLARNRDEAVVLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAILGATVVLKGHKTLVAASDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     SAGEIAPAARWLRNWITESM"
     gene            27942..28598
                     /locus_tag="ACSIGJ_000029"
     CDS             27942..28598
                     /locus_tag="ACSIGJ_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HHQ1329042.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LLVLFLLVFRLCWGIWGSDTARFSRFVRGWAGIRGYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLIRKIHLNFFKLLAVFSAVH
                     IAAVAAYRIFKKKNLVRPMITGFKYIEGKTSIRFAGKAALAAALSVAALAAAAILLLS
                     "
     gene            29027..30109
                     /locus_tag="ACSIGJ_000030"
     CDS             29027..30109
                     /locus_tag="ACSIGJ_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-alpha C-terminal
                     domain-related protein"
                     /protein_id="HHQ1329043.1"
                     /translation="MNYFPIFANLSGRPVLVVGGGAVAARKISLLPKAGAEVGVAAKH
                     LNAELSALAAENKILWLAKEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VGDRDHCSFIFPSVIDRDPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAGFYFPVQLVVRQDADKADGFRGYQGRIERGSVTVGQTVRIEPNGLTAEVSEIIA
                     PKGEVAQAFAGEAATIRLDRDIDVSRGDLFVDKNFPLAPQKHPEATLCWFDERPLNTA
                     RKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRINLQKPVTATPY
                     AENTAAGSFILIDEATYGTVAAGMIL"
     gene            30106..31899
                     /locus_tag="ACSIGJ_000031"
                     /pseudo
     CDS             30106..31899
                     /locus_tag="ACSIGJ_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
     gene            31926..33691
                     /gene="cysI"
                     /locus_tag="ACSIGJ_000032"
                     /pseudo
     CDS             31926..33691
                     /gene="cysI"
                     /locus_tag="ACSIGJ_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
     gene            33864..35723
                     /gene="ilvD"
                     /locus_tag="ACSIGJ_000033"
     CDS             33864..35723
                     /gene="ilvD"
                     /locus_tag="ACSIGJ_000033"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HHQ1329044.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQDDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVYTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRIWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEAAVEDILGNQIVAGNIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTIEIDIPKRSIRLVISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVSQIER"
     gene            35765..36067
                     /locus_tag="ACSIGJ_000034"
     CDS             35765..36067
                     /locus_tag="ACSIGJ_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329045.1"
                     /translation="MIINKFIWAMAVFSAILALVIGSGFTALEYVKEPPAAPYAVSAP
                     KTAGVKPRRILPERNPPCCRLTVQTPRPFQTPAMRTDRIRLTERQQIPFPSSGSAA"
     gene            36105..36878
                     /locus_tag="ACSIGJ_000035"
     CDS             36105..36878
                     /locus_tag="ACSIGJ_000035"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HHQ1329046.1"
                     /translation="MQTTKICYAFRTPSAYGQYLADAGFDYLSLANNHSNGFGAQGIT
                     ATAGNLDELNIKYSGIENRFETAILKKNGVRYGFVSFAPNLAAVKLNDYAKFKKLIRK
                     TKQKTDIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGADVVFGQG
                     AHVTRAVELYHDRFISYSGGNFATYGISGIAPIFKIITDKQGRFVSGNIIPITQVGDK
                     IPKIDPEKTVIERIIYLNHSDFPNGNGLDVSPGGDITRR"
     gene            complement(36974..38026)
                     /gene="bioB"
                     /locus_tag="ACSIGJ_000036"
     CDS             complement(36974..38026)
                     /gene="bioB"
                     /locus_tag="ACSIGJ_000036"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HHQ1329047.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLELVYQAA
                     EVHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTVSVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKADY"
     gene            complement(38233..39609)
                     /gene="mpl"
                     /locus_tag="ACSIGJ_000037"
     CDS             complement(38233..39609)
                     /gene="mpl"
                     /locus_tag="ACSIGJ_000037"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HHQ1329048.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFKVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFQADIYVIGNVARRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDPN
                     SKSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGQQQSLQDTLDKGCWTPVEKFGTGHGWQIGEVNADGSFDVLLDGK
                     KAGHVAWDLMGGHNRMNALAVIAAARHAGVDVQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKSALPASLKEADQ
                     VFCYAGGADWDVAEALAPLGCRLRVGKDFDTFVAEIVKNARTGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            complement(39671..39976)
                     /locus_tag="ACSIGJ_000038"
     CDS             complement(39671..39976)
                     /locus_tag="ACSIGJ_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HHQ1329049.1"
                     /translation="MRVSKMIGSILLVAAVQTVFSANVYECRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEAVQNPKGNAQKDGSDAGIRPH"
     gene            complement(39988..40341)
                     /locus_tag="ACSIGJ_000039"
     CDS             complement(39988..40341)
                     /locus_tag="ACSIGJ_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329050.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKYRLVVIANSAWGLLLNLP
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEKQ"
     gene            complement(40603..40800)
                     /locus_tag="ACSIGJ_000040"
     CDS             complement(40603..40800)
                     /locus_tag="ACSIGJ_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329051.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILSSNAVAAAVLFWIWWKWF"
     gene            complement(40810..41595)
                     /locus_tag="ACSIGJ_000041"
     CDS             complement(40810..41595)
                     /locus_tag="ACSIGJ_000041"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HHQ1329052.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGRKPVGVYIPEAKMPSEEVLKLTAVLPEDGIFSVSDGILKKISSLSCADDILTLIDI
                     PLGGTLPDKGDCAVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGCADAWSPKVLRAGM
                     GAHFLLDIYSQADLEIWLAHYEDRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            complement(41669..42979)
                     /gene="tilS"
                     /locus_tag="ACSIGJ_000042"
     CDS             complement(41669..42979)
                     /gene="tilS"
                     /locus_tag="ACSIGJ_000042"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HHQ1329053.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVCA
                     GKRAGFVPEALHIHHGLSPRADDWADFCRNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAEFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPLGENGIIWR
                     PLLPFSRQDIWDYARKHGLPNIEDESNTDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLEEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRNFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFASEQLAKPAFLKDGTISGNLK
                     KILTEHRFVLKRHPFGLPEAMLEQDGILRTVAASDTLAVGGIHKNVKKILQGKRVLPF
                     LRPIWPLVADSGNRPLALANCCADFQISVSDGILPVHPDFPILF"
     gene            complement(43047..44006)
                     /locus_tag="ACSIGJ_000043"
     CDS             complement(43047..44006)
                     /locus_tag="ACSIGJ_000043"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HHQ1329054.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIDALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVVVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            44218..44619
                     /locus_tag="ACSIGJ_000044"
     CDS             44218..44619
                     /locus_tag="ACSIGJ_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HHQ1329055.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPHKIKVKGLNHQRRPAPEARLLYEEDAKTAALREECKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            complement(44687..44884)
                     /gene="iscX"
                     /locus_tag="ACSIGJ_000045"
     CDS             complement(44687..44884)
                     /gene="iscX"
                     /locus_tag="ACSIGJ_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HHQ1329056.1"
                     /translation="MKWTDTRRIAEELYDLHGEAIDPKTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            45097..45637
                     /locus_tag="ACSIGJ_000046"
                     /pseudo
     CDS             45097..45637
                     /locus_tag="ACSIGJ_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(45697..46038)
                     /gene="fdx"
                     /locus_tag="ACSIGJ_000047"
     CDS             complement(45697..46038)
                     /gene="fdx"
                     /locus_tag="ACSIGJ_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HHQ1329057.1"
                     /translation="MPKITVLPHATLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPAELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(46094..46855)
                     /locus_tag="ACSIGJ_000048"
     CDS             complement(46094..46855)
                     /locus_tag="ACSIGJ_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HHQ1329058.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIS
                     ALDFAMVKVPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGGLTNVEQGESVCV
                     PVGDISDWMFVCNGIPYGGFTVQAMRGQMTEEERTEHDTAWGIDFGDPGQVLPVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAMAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            complement(46891..47497)
                     /locus_tag="ACSIGJ_000049"
                     /pseudo
     CDS             complement(46891..47497)
                     /locus_tag="ACSIGJ_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(47725..49587)
                     /gene="hscA"
                     /locus_tag="ACSIGJ_000050"
     CDS             complement(47725..49587)
                     /gene="hscA"
                     /locus_tag="ACSIGJ_000050"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HHQ1329059.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDADGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRFIELHTRQGVKTPVEVSAEILKTLKLRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCYLLEQNRLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATFSDGMAIDTSISRAEFHNLTQHLVMKTFEPVKQALKDAGVGKNEVK
                     GVVMVGGSTRMPHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKADGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDDTITQMLKDSMGNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEEFAQI
                     QRDIADLQGRLKDGKAEDIRAAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            49758..50630
                     /gene="hpnD"
                     /locus_tag="ACSIGJ_000051"
     CDS             49758..50630
                     /gene="hpnD"
                     /locus_tag="ACSIGJ_000051"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046905 - 15-cis-phytoene synthase
                     activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HHQ1329060.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKQDAVTVLYVFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDNAFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYALLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            50705..52018
                     /gene="hpnE"
                     /locus_tag="ACSIGJ_000052"
     CDS             50705..52018
                     /gene="hpnE"
                     /locus_tag="ACSIGJ_000052"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HHQ1329061.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAILATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGIADGTAQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLRRILPHLGEPEAVRVITEKRATTATDAPPPDL
                     SWLHRHRIFPAGDYLHPHYPATLEAAVQSGFASAEACLQSLNDAV"
     gene            52100..52819
                     /locus_tag="ACSIGJ_000053"
     CDS             52100..52819
                     /locus_tag="ACSIGJ_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HHQ1329062.1"
                     /translation="MPTLTDKTILVTGASQGLGEQVAKAYAAEGATVILVARHQKKLE
                     KAYDAIVEAGHPEPFAIRFDLMSAEEKEFERFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEARSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            52985..53656
                     /locus_tag="ACSIGJ_000054"
     CDS             52985..53656
                     /locus_tag="ACSIGJ_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HHQ1329063.1"
                     /translation="MKTVSAAIAFAAAAVSLTGCATESSRSLEVAKVASCNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDSEDRLGSQAKTILVTHLQQTNRFNVLNRTNLSALKQE
                     SGISGKAQNLKGADYIVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSTQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVDNLVQAVDNGA
                     WQSNR"
     gene            53666..54032
                     /locus_tag="ACSIGJ_000055"
                     /pseudo
     CDS             53666..54032
                     /locus_tag="ACSIGJ_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
     gene            54029..54676
                     /locus_tag="ACSIGJ_000056"
     CDS             54029..54676
                     /locus_tag="ACSIGJ_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HHQ1329064.1"
                     /translation="MKPLILGLAAVLALSACQVRKAPDLDYTSFKESKPASILVVPPL
                     NESPDVNGTWGMLASTAAPISEAGYYVFPAAVVEETFKENGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVGAVVNQIANSLTDRGYQVSKTAAYNLLSPYSRNGILKGPRFVEEQPK"
     gene            55069..56835
                     /locus_tag="ACSIGJ_000057"
                     /pseudo
     CDS             55069..56835
                     /locus_tag="ACSIGJ_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707572.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            56939..57607
                     /gene="hda"
                     /locus_tag="ACSIGJ_000058"
     CDS             56939..57607
                     /gene="hda"
                     /locus_tag="ACSIGJ_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HHQ1329065.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLQHKHDPFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     NMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            57604..58272
                     /locus_tag="ACSIGJ_000059"
     CDS             57604..58272
                     /locus_tag="ACSIGJ_000059"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HHQ1329066.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKLHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGIANMIGTQLETGPDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVSEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            complement(58863..59759)
                     /gene="argB"
                     /locus_tag="ACSIGJ_000060"
     CDS             complement(58863..59759)
                     /gene="argB"
                     /locus_tag="ACSIGJ_000060"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HHQ1329067.1"
                     /translation="MEFENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKETMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLVDTPEQNSVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDGLIADGTLYGGMLPKIASAVEAAANGVKATHIIDGRLPNA
                     LLLEIFTDAGIGSMILGRGEDA"
     gene            59929..61059
                     /locus_tag="ACSIGJ_000061"
     CDS             59929..61059
                     /locus_tag="ACSIGJ_000061"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HHQ1329068.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDMKI
                     WITARQHPGETMAEWFVEGLLGRLLDSQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVRGKMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALEAQFKTALLNASPDFQDEYGYEKDAPGKANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAVLSAILNVAGDLR"
     gene            61133..61984
                     /gene="lgt"
                     /locus_tag="ACSIGJ_000062"
     CDS             61133..61984
                     /gene="lgt"
                     /locus_tag="ACSIGJ_000062"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HHQ1329069.1"
                     /translation="MIIHHQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFSRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRITDINAFWA
                     MGFPQAHYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKP
                     RPTGQTAALFLGGYGVFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(62069..62731)
                     /locus_tag="ACSIGJ_000063"
     CDS             complement(62069..62731)
                     /locus_tag="ACSIGJ_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HHQ1329070.1"
                     /translation="MDSRKFTEASKRRLGELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLAPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYDDGEG
                     GSDFYTWCNAYLYGLDIVPTDWFEAADDEAFEELFYPVMALGGIYDEEENGAIRLQFT
                     EGELAELASELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            62765..62871
                     /locus_tag="ACSIGJ_000064"
                     /pseudo
     CDS             62765..62871
                     /locus_tag="ACSIGJ_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(62930..64723)
                     /locus_tag="ACSIGJ_000065"
     CDS             complement(62930..64723)
                     /locus_tag="ACSIGJ_000065"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HHQ1329071.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKRHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGADIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGKAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSQIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWSANRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGNMNAESYKFISQKISTETGEEPRADIVFGIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVANGQGADTD
                     ILAATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            64969..66078
                     /gene="proB"
                     /locus_tag="ACSIGJ_000066"
     CDS             64969..66078
                     /gene="proB"
                     /locus_tag="ACSIGJ_000066"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HHQ1329072.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAIPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDSLAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            66119..67354
                     /locus_tag="ACSIGJ_000067"
     CDS             66119..67354
                     /locus_tag="ACSIGJ_000067"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HHQ1329073.1"
                     /translation="MPKRQKKSVNTADAEEKNRALLAMADSLEAAAEDILAANRLDLK
                     AAAGKIPDSMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPNGLEIVKKRV
                     AMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAALKTGLAQTRI
                     DPEAVQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPVIETGTGIVH
                     IYIDKDADWDKALRIVYNAKTGRPSVCNSMEVLLVHEGIAADFLPKLERLLVRGRIEA
                     GLPPVRFRLDPQAARYIGGEAAGADDFDTEFLDYILAVKTVASVEEAVGHIEARGTHH
                     SDGIVTENRHAADYFTTHIDSAAVYINASTRFTDGGEFGLGCEMGISTQKLHARGPMG
                     LKELTSYKYIVQGTGQVRE"
     gene            complement(67530..70574)
                     /locus_tag="ACSIGJ_000068"
     CDS             complement(67530..70574)
                     /locus_tag="ACSIGJ_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HHQ1329074.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPEFSLMLFHAVKTAVYWLFVGAVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMQSESKTSPVRPVFKEITLEEATRALSSAALRETKKRYIDAFEKNGTAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQEKGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEA
                     RISRLIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIHIEEPAAPDA
                     WVVEPPEVPEVAVPEIDILPPPPVSEIYNRTYEPPAGFEQAQRSRIAETDHLAADVLN
                     GGWQEETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAVCPFEDVPSERPSCRVSDT
                     EADEGAFQSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVK
                     VKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGL
                     ELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTG
                     SGKSVGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGITHLLAPVVTDMKLAANA
                     LNWCVNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLP
                     FIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRV
                     VEYLKQFGEPDYVDDILSGGGSEELPGIGRSGDGETDPMYDEAVSVVLKTRKASISGV
                     QRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            70739..71419
                     /locus_tag="ACSIGJ_000069"
     CDS             70739..71419
                     /locus_tag="ACSIGJ_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329075.1"
                     /translation="MIFQTVWFSDVVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASVGGGQLAQMNYHLLAVNLVTLMVDTSAALWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALVVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     IGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSERGIWL"
     gene            71448..71807
                     /locus_tag="ACSIGJ_000070"
     CDS             71448..71807
                     /locus_tag="ACSIGJ_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HHQ1329076.1"
                     /translation="MLSNILPLSIGAIFGTTARWLLNLAVPASLSPATGNLFANWTGA
                     LLIGIFAETVSHPQWKLLLITGFFGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGTAVK"
     gene            71885..72018
                     /locus_tag="ACSIGJ_000071"
                     /pseudo
     CDS             71885..72018
                     /locus_tag="ACSIGJ_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(72412..72948)
                     /locus_tag="ACSIGJ_000072"
     CDS             complement(72412..72948)
                     /locus_tag="ACSIGJ_000072"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HHQ1329077.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLSNDEDEITETVLMSKDEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(72982..73338)
                     /gene="folB"
                     /locus_tag="ACSIGJ_000073"
     CDS             complement(72982..73338)
                     /gene="folB"
                     /locus_tag="ACSIGJ_000073"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HHQ1329078.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVHVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            73394..73996
                     /gene="plsY"
                     /locus_tag="ACSIGJ_000074"
     CDS             73394..73996
                     /gene="plsY"
                     /locus_tag="ACSIGJ_000074"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HHQ1329079.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALVATTAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNILNLIKGKESKIGEKR"
     gene            74088..74933
                     /locus_tag="ACSIGJ_000075"
     CDS             74088..74933
                     /locus_tag="ACSIGJ_000075"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HHQ1329080.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGLYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLIRIGDKLRLFELHPADFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     VILIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLYAELHVHAPKTDGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DNKIR"
     gene            complement(75042..75374)
                     /locus_tag="ACSIGJ_000076"
     CDS             complement(75042..75374)
                     /locus_tag="ACSIGJ_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGK_0946 family protein"
                     /protein_id="HHQ1329081.1"
                     /translation="MKNLKNISVVAVCAVLLAACASENSVANYAIGDDSAVIKAGRNR
                     AEARISRAELAQHRRQRKNVSEELALEREKRANKHDAIRQGMGTAAGGLMLLNGVVGT
                     VGVMKSVF"
     gene            complement(75424..75672)
                     /locus_tag="ACSIGJ_000077"
     CDS             complement(75424..75672)
                     /locus_tag="ACSIGJ_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329082.1"
                     /translation="MKMKKLILLSVAAMLVTACTYADRRFVTQESAAEIQAKSRAIQI
                     SERAERAEYRKERREEMMDAARAIKKANENSPNIYFIR"
     gene            75915..76889
                     /locus_tag="ACSIGJ_000078"
     CDS             75915..76889
                     /locus_tag="ACSIGJ_000078"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HHQ1329083.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDTFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILAKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYVFTLNAENEFVLTKENPKVPESTKEFAINMSNRRHWLPPVQQYVDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYLQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNAYQAMLDIQPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(77048..77266)
                     /locus_tag="ACSIGJ_000079"
     CDS             complement(77048..77266)
                     /locus_tag="ACSIGJ_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HHQ1329084.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLANNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
     gene            77460..77723
                     /locus_tag="ACSIGJ_000080"
     CDS             77460..77723
                     /locus_tag="ACSIGJ_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017509996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HHQ1329085.1"
                     /translation="MKGTPQFPQCGFSARAVEVPTQIGRPFAFVNILENPEIRSTLPL
                     IANWPTFPRLWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
     gene            77891..79659
                     /gene="ggt"
                     /locus_tag="ACSIGJ_000081"
                     /pseudo
     CDS             77891..79659
                     /gene="ggt"
                     /locus_tag="ACSIGJ_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            79723..79887
                     /locus_tag="ACSIGJ_000082"
     CDS             79723..79887
                     /locus_tag="ACSIGJ_000082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329086.1"
                     /translation="MSNQTKIQAIKQASEQILTICETPNTALQAIHLILRHGGAGRIV
                     LASGVSAGYG"
     gene            79832..80074
                     /locus_tag="ACSIGJ_000083"
     CDS             79832..80074
                     /locus_tag="ACSIGJ_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329087.1"
                     /translation="MAGQEELSWQVVYQRVMADKDVVGAGYLIDFAQTAENLPFDVLP
                     LISLVLNKGDETLKTGMLNKLPDNAKENLRIMGYLP"
     gene            80141..81391
                     /gene="glyA"
                     /locus_tag="ACSIGJ_000084"
     CDS             80141..81391
                     /gene="glyA"
                     /locus_tag="ACSIGJ_000084"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HHQ1329088.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDRRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPAKRYYGGCEYVDIVEQLAIDRVKELFGAAYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAVMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNETDARVLSNLVADVLANPE
                     DEANLAKVRGQVTALCDKYPVYGN"
     gene            complement(81464..81853)
                     /locus_tag="ACSIGJ_000085"
     CDS             complement(81464..81853)
                     /locus_tag="ACSIGJ_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHQ1329089.1"
                     /translation="MEVHDKIRTLREVNQWTQEEMAEKLEMSVNGYSKIERGKSGINL
                     DKLRQIAQIFNIDVVELLAEQNRSFFFSIGDNTNNHHNIIGSDEMLVFENEKLRSLLD
                     AKDELIRQKDSEIAVLKKLVILLEEKK"
     gene            82793..83584
                     /locus_tag="ACSIGJ_000086"
     CDS             82793..83584
                     /locus_tag="ACSIGJ_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HHQ1329090.1"
                     /translation="MKKALLALTIAAISGTAMAQLPDFLGKGEYTVRTDISKQTLKNA
                     DLKEKHKVQKNIGFRADMPFDDIHHGMRFEVSHSRDKKDMYVVTESTTKPFGKDVKEK
                     RTDVYAGYTYTQPLSEATKLRAGLGLGYEKYKDAVANEKGTVSTEREAFYTKAHADLT
                     SDLGGGWYLNPWAEVKVDLDAKLKHNATVAGVSADINAKTRGWGVGVGANIGKQITDT
                     VGIEAGPFYKHRHFKASGSFVLDGGNIRVDPTKINEYGVRVGVKF"
     gene            83735..84379
                     /locus_tag="ACSIGJ_000087"
     CDS             83735..84379
                     /locus_tag="ACSIGJ_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HHQ1329091.1"
                     /translation="MLASVIDFILHIDQHLLSLSAQYGVWIYAILFLIVFCETGLIVT
                     PFLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRCEYLGKTRRFYEKHGGKTIIIARFVPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGIIEIARAKLAAKSER"
     gene            84398..86308
                     /locus_tag="ACSIGJ_000088"
     CDS             84398..86308
                     /locus_tag="ACSIGJ_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HHQ1329092.1"
                     /translation="MNILSVENASFAVGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILTGVQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEICDLLRRYH
                     HVSHELENGSSELLLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGKKSGKIIAELEHASFAYDDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     VFEGQGRLKEYIGGYQDYIDAKSRENKIQTASAPKASDVEPAKEKPKANRTVKLSYKE
                     QRELDALPDEIAALETEQAEINAQLSDPGIFKDYEKAGALQNRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            86330..86696
                     /locus_tag="ACSIGJ_000089"
                     /pseudo
     CDS             86330..86696
                     /locus_tag="ACSIGJ_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            86808..88283
                     /gene="trpE"
                     /locus_tag="ACSIGJ_000090"
     CDS             86808..88283
                     /gene="trpE"
                     /locus_tag="ACSIGJ_000090"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HHQ1329093.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKAGGKHVDVYQNGEIVEQYDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETVYNFEHFAHRLKNTAKANPLGTPDILLMLSQEL
                     AVIDNLSGKIHLIVYADPSQPDSYERARERLEDIRTQLRQSCAIPLSLGSKQTQAVSE
                     FGEEPFKACVDKIKDYIFAGDCMQVVPSQRMSMEFTDNPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGCLKEGVTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEIEPEKRGIYGGAVGVWGFNNDMDLAIAIRTAVIKNNTL
                     FIQSGAGVVADSDPTSEWQETQNKARAVVRAAQMVQEGLDK"
     gene            88367..89305
                     /locus_tag="ACSIGJ_000091"
     CDS             88367..89305
                     /locus_tag="ACSIGJ_000091"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HHQ1329094.1"
                     /translation="MQFTSLEICAGAGGQALGLERAGFSHVALIEIEPSACQTLRLNR
                     PDWNVIEGDVRLFQGEGYDGIDLLAGGVPCPPFSKAGKQLGKDDERDLFPEAIRLAKE
                     TDPKAIMLENVRGLLDPKFENYRNHITEQFAKLGYLGQWKLLYAADYGVSQLRPRVLF
                     VALKNEYTNFFKWPEPNSEQPKTVGELLFDLMSENNWQGAHNWRLKAAQIAPTLVGGS
                     KKHGGADLGPTRSKRAWAELGVDGSGLWDSAPPEDFSGMPRLTVRMTARIQGFPDNWQ
                     FFGKKTPMYRQIGNAFPPPVAEAVGRQIIKALKKEN"
     gene            89306..90166
                     /locus_tag="ACSIGJ_000092"
     CDS             89306..90166
                     /locus_tag="ACSIGJ_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoMIV family type II restriction endonuclease"
                     /protein_id="HHQ1329095.1"
                     /translation="MNPLFTQERRIFHKKLLDGNILATNNRGVVSNADGSNTRSFNIA
                     KGIADLLHSETVSERLPGQTSGNAFEAICSEFVQSAFEKLQHIRPGDWNVKQVGSRNR
                     LEIARYQQYAHLTALAKAAEENPELAAALGSDYTITPDIIVTRNLIADAEINRNEFLV
                     DENIATYASLRAGNGNMPLLHASISCKWTIRSDRAQNARSEGLNLVRNRKGRLPHIVV
                     VTAEPTPSRISSIALGTGEIDCVYHFALYELEQILQSLNYEDALDLFYIMVNGKRLKD
                     ISDLPLDLAV"
     gene            90183..91319
                     /locus_tag="ACSIGJ_000093"
     CDS             90183..91319
                     /locus_tag="ACSIGJ_000093"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HHQ1329096.1"
                     /translation="MNTPPILPPAMLGILGGGQLGRMFAVAAKTMGYKVTVLDPDPNA
                     PAAEFADRHLCAPFDDRAALDELAKCAAVTTEFENVNADAMRSLAKHTNVSPSGDCVS
                     IAQNRIQEKARIRKAGLQTAPYQAVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTLDELKAAFAEHGGVDCVLEKMVDLRGEISVIVCRLNDENVQTFDPAENIHENGILA
                     YSIVPARLSADVQQQARQTAQRLADELDYVGVLAVEMFVVGDTHELLVNETAPRTHNS
                     GHHTIDACAADQFQQQVRIMCNLPPADTKLLSPCCMANILGDVWQEDGGEPDWLPLQS
                     RPNAHLHLYGKKTAQKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            91366..91848
                     /locus_tag="ACSIGJ_000094"
     CDS             91366..91848
                     /locus_tag="ACSIGJ_000094"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHQ1329097.1"
                     /translation="MSLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LRQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            91954..93002
                     /locus_tag="ACSIGJ_000095"
                     /pseudo
     CDS             91954..93002
                     /locus_tag="ACSIGJ_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
     gene            93582..94187
                     /locus_tag="ACSIGJ_000096"
     CDS             93582..94187
                     /locus_tag="ACSIGJ_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HHQ1329098.1"
                     /translation="MNTDFQKILKQLGKQAKKEAAEKQEAEKNKQKQEYDLDFSQAVG
                     QVSPLKNRQQYYAQSDKTPIKARPKDNRTDEENYFYIGSTYNDPPASFCKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYVEPREGNDGCVRILLKRKLRQQD"
     gene            94250..94612
                     /gene="ssrA"
                     /locus_tag="ACSIGJ_000097"
     tmRNA           94250..94612
                     /gene="ssrA"
                     /locus_tag="ACSIGJ_000097"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT        23483 a        26741 c        23344 g        21328 t
ORIGIN      
        1 tcttatgcca ctcatgcaca cccataaacc ccagcagcag attgagcggc agaaaaaacg
       61 gtttgccgtc cgcctctccg tacacgtcga gcaaagcggc gcgtatatcg ggcgcgcgtt
      121 tgttgtccaa cacgaaaccg gggcggattt caacggcaag catattcaga atacggctct
      181 gttgcgcctg cttcatacgg tgggcgtgga aaagggcggc gatatccgca tcggaacgga
      241 cggcggcagg tttgcggacc ttcttcttca ttgccgaaag cacctgtttg gcattgtgga
      301 aatcgccctg ccagacagtt gcaatatttt gataggtagc cttcaaaatg ccgtctgcac
      361 cgctttcgcg gacataatac caacctttgg gcggcttttg cagactttcg ttgcgccatt
      421 cgaacccgtt atcgggaaaa ataaaagaag acatgggata cctgcgtcat gttttgaaaa
      481 tagggcggca gaaccgcaaa ccatacggat ggtacagcaa ggagcggcaa cacagaactg
      541 tttttttgtt cccgccttgt ctttccaagc ccatgccgtc tgaagccgga atgtttcaga
      601 cggcatggca tcaaactcca taaataaacc gcgtatgctt gaaataatac tttcaacccc
      661 aatttacgcg gcaatccgta tcctgtcaga cacaagagag tatccatgac acaaaaagaa
      721 aagcattttg aggaatatgc tgctttggcg acccttcctt tacgggatgt cgtcgtttac
      781 ccgcatatgg ttctgccgct gttcgtcggc aggccgaaat ccatcgccgc actggaaaac
      841 gccattaccc gcgaggaacc ggttttcctg ttggcgcaaa ccgatgcggc ggtagaagaa
      901 ccggttgccg ccgacctgta tcagaccggt accgtcgcac aagtcctgca agtgttgaaa
      961 ctgcccgacg gcacggtaaa agtgctggtc gaagggctgt atcgcggacg tgttttgacc
     1021 atcgaagaca cgggcgggct gttcgtttct catatagagg cggtcgtgga agaagacacg
     1081 ggcggcaata ccgacctcga agccgtgcgc cgcaccctgt tggcgcagtt tgaacaatac
     1141 gccaaactca ataaaaaaat ccctgccgaa attatcggca gcatcaacgg cattgccgaa
     1201 aacagccggc taaccgatac ggtcgcagcg catttgcagt tgaaactggc gcaacgccaa
     1261 cagattttgg agattcccga aatcggcaaa cggatggaat tcctgctggc aaagctggaa
     1321 tccgaactcg acattatgca ggccgaaaaa cgcatacgcg gacgcgtcaa acgccaaatg
     1381 gaaaaatccc aacgcgaata ttatctgaac gaacagatta aagcgataca caaagaattg
     1441 ggcgaagaag acgaaaacgg cgaactggat gccttggagg caggcatcaa aaaggcgggt
     1501 atgaccaaag aagcggaaga aaaatgcctg tccgaactga aaaaactcaa aatgatgccg
     1561 ccgatgtctg ccgaatccac cgtcgtacgc aactacatcg acaccctgct cggactgccg
     1621 tggaagaaaa aatcccgcgt cagcaaagac atcgccaaag ccggactggt gctggatgcc
     1681 gaccactacg gcctggaaaa agtcaaagaa cggattttgg aatacctcgc cgtccaaaaa
     1741 cgtatggaca agctcaaagg tccgattctg tgtttggtcg gccctccggg cgtgggcaaa
     1801 acctctttgg gcgaatccat cgccaaagca acggggcgga aatatgtccg tatggccttg
     1861 ggcggcgtgc gcgacgaaag cgaaatccgc gggcaccgcc gtacctatat cggctctatg
     1921 cccggcaaga ttttgcagaa tatggcaaaa gcaggcgtaa gaaacccgtt gttcctgctc
     1981 gacgaaatcg acaaattggg cagcgacttt cggggcgatc ccgccagcgc gttgcttgaa
     2041 gtgctcgatc acgaacaaaa caacaagttc gccgatcatt atgccgaagt ggattacgat
     2101 ttgagcgacg tgatgtttat tgcgacttca aacagcctga atatcccgac cccgctgctt
     2161 gaccgtatgg aaatcatccg tctgtccggc tataccgaag acgagaaaat caatatcgcg
     2221 atgcagtatc tcgtaccgaa gcaaatgaaa cgcaacggcg tgaaagaagg cgaattggtg
     2281 gtcgaagaaa gcgcggtacg cgatattatc cgttattaca cccgagaggc gggcgtgcgt
     2341 tcgctcgacc gcgaaattgc caaaatctgc cgtaaggtgg tgatgcagat taccttgaac
     2401 gaagataaga agaggttgtc tgaaaccaag aaaaccagca aagccaagcc tagggcggtt
     2461 aaagtcaatg agaaaaacct acatgactac ttgggcgtgc gccgcttcga ttacggcgtt
     2521 gccgaaagtg aaaaccgcat cggacaggtt accggtttgg cttggacgga agtcggcggc
     2581 gaattgctga ctgtcgaagc cgcagcatta ccgggtaaag gcatgatcca gtgtaccggc
     2641 cagttgggcg atgtgatgaa ggaatctgta tccgctgcat ggtctgttgt ccgttcccgt
     2701 gcggaatcag taggtttggc tcctgatttt tacgagaaaa aagacattca tgtccatgtt
     2761 cccgaaggtg caacgccgaa agacggtccg agcgcaggca ttgctatgac tttggcgatg
     2821 gtgtccgcct ttaccaaaat tccggtgcgt gccgatgttg ccatgacggg cgaaattacc
     2881 ctgcgcggcg aagtcctgcc gatcggcggt ctgaaggaaa aactgttggc tgcgcttcgc
     2941 ggcggcatca aacacgtcct gattccgaaa gacaatgtca aagacctgga agaaatccct
     3001 gaaaacgtga aaaccggatt aaccatccat ccggtcaaat ggatagacga ggtattggct
     3061 ttgggtttgg aaagccggcc tgagtcttgg gcagaacctt ctgccgcgga agcggcggcg
     3121 gaatccgctt caaaaccgaa accccgcagc agggcaatca aacattgaaa cgcaggaaat
     3181 gtgttgtaaa aatgcggttt cgtcctgaaa gcctgtaaaa tagggcgatt ccgtattttt
     3241 tgcttgacac ggcaatttca gaattgctat aaagcgaaag ttgctcaagc agtacaaacc
     3301 ccgagtattc gggcatttca tcatttttta agacaggaag ggactgattg tgaacaagtc
     3361 tgaattgatc gaagcgattg ctcaagaagc cgatatttcc aaagccgccg cacaaaaagc
     3421 tttggatgcc actaccaacg cagtaaccaa cgccctgaaa caaggcgaca ccgttactct
     3481 ggtcggtttc ggtactttct acgtcggcga acgtgcggaa cgccaaggcc gcaaccccaa
     3541 aaccggcgag cctctgacca ttgccgccgc caaaacgctt aaattccgtg caggtaaagc
     3601 attgaaagac gcactgtaaa ctgtttttca gcaaaagccg attccttgaa gaatcggctt
     3661 tttgttttcc ccgccgaatc aggatagggg tttgcccgtc ggattcggat gaagggctgc
     3721 ccgtcaaacc cggatggaaa aatgccatct gaaacgcttt tccccgcgtt cagacggcat
     3781 tccagacctg aggttcaatc ggctttcaaa tccgcgacac attgtttgtc tcgagtttca
     3841 aacgcgtttg cgccgccgag caggtcaagc tgttcttgtg caccgagttt gccgaaggag
     3901 ctgatctgtt tctcgctcaa tccgtccaaa ggctgctccc acatacattt gcagtagtcg
     3961 acggcgagac gggtattgtc cgcatccaag ccgcgcgctt tcaaatcgtt ctgccatttt
     4021 tcagcaaacg gaatattttt catgcaagac tcgacaattt tctgttttgc ctgcggtttg
     4081 gacatcgcgc attgggagag cagggcggtt gaagcgagca acgccaaaat gacccacgcc
     4141 caaatgcgga tggtgcggat tttggctttt gcttttttgc gcgcggcaac ctgctcttcc
     4201 gtcggcgttt cgtgtttcgg ctcagtcatg caggctttcc atgcggatca tggtaatcgg
     4261 tttttccaca caatccagtg cttcgatggc tgcgattgcc gacttgatgt gtttttcgac
     4321 cgtgctgtgg gtcagaatca cgatttcggc agtggtctga tcgataacgc ctttttggat
     4381 taaggcttcg atggacacgt tttcttgtgc caacagcgcg gcgatttgcc ccagcgtgcc
     4441 cggttcgtct ttggcttgga cgcgcaggta gtagctgctg gtaatttcgt ccataggcag
     4501 gatggtttgc gcttggactt gggcgggttg gaacgccaga tgcggtacgc ggtgggcggt
     4561 gtcggcttca accaggcggg cgatgtcgat gatatcggca accacggcgg aagcggtcgg
     4621 caatgcgccc gcgcccgcgc cgtaatataa ggtttcgccg accatattgg cgttgacgcg
     4681 cacggcgttc atcacgccgt cgacgtttgc caagaggcgg ctttcgggaa tcagggtcgg
     4741 atggacgcgc aattcgatgc ctttgccggt tttgcgggtg atgcccaaca gtttgatgcg
     4801 gtagccgagt tcttcggcgt atttgatgtc gcggctgtcg agtttgctga tgccttcgag
     4861 atagcaggcg gagaagttca tcggcgtacc gaatgccagc gcgctcatga tggtgatttt
     4921 atggcctgcg tcgttgcctt cgatgtcgaa ggtcgggtcg gcttcggcat aacccaatgc
     4981 ctgcgcttct ttcaatacgt cggcaaacgc gctgcctttt tcgcgcattt cggagaggat
     5041 gaagttgctg gtgccgttaa taatgccggc gatggattta atcctgtttg ccgccaaacc
     5101 ttcgcgcagg gctttgatga ttgggatacc gcccgctact gccgcttcaa attggacgat
     5161 gacgttttgt ttttccgcca gcgggaagat ttcgttgccg tattcggcga gcagtttttt
     5221 gttggcggta acgatgtgtt tgccgttttc aatggctttc aacaccgctt ctttggcaat
     5281 gcccgtgccg ccgaacaatt cgacgacgac atcgacgtct ttacgcgcga ccagttcgaa
     5341 cggatctttg acaaaggcgg cggacgggca gatttggcgg gctttttctt cgcttaagtc
     5401 gcacatggca gaaatacgga tttcgcgccc caagcggcgg gaaatttcct ctgcgttgtc
     5461 ccgcaacacg gcagccgcac caccgccgac cgtacctaag cctaaaagac cgatgtttac
     5521 tggcttcatt gtgtctcctt gtaagccgac tgaaatgtaa atattgaaag acgaaaatat
     5581 ccgctgccgg tataattgtg ccgcactttg aatcaaatgc cgtctgaaat cggcaggcgc
     5641 gtcagatgaa atctgccaat cctacattaa ttcgcctgat tttgcacccc tttcggtgca
     5701 gatgatgcag caacggggta aaaaatgttg tttgaagaaa atccgataga cggacagttt
     5761 gcagaatatg aatgcggcgc gggcggaatc cggctggcgg ggcaaagttt ccataaaccc
     5821 gtgcttgtac ataaggattc ggtctgcctg ccgcaatgcc gaaccttgtc cgacctgact
     5881 ccggaaaacc tgttgtccga catcaaacct gttgactatc cggaaatatt gattatcggg
     5941 acgggcgcgg ctcaggagtt tatccatccc aaaatcatgg cggatttttc ccgaatcgga
     6001 atcagcgtgg aatgcatgaa taccgattcg gcattcagga cattggtttt cctgcactcg
     6061 gaagggcgca gggcttgggc ttggcttcag ccgtaaattt cccgtttcag acggcatcgg
     6121 cactgacttt caggtaaaat acgggctttt cccgcccgac gatgtttccg ttatgattga
     6181 aatcaaaaac ctcaccctgc aacgcggttt gaaagtcctg ctcgacaagg cttccgctgc
     6241 cgtcaatccc ggtcagcgcg tcggtttgat cggtaagaac gggacgggca aatccagcct
     6301 gtttgcctta atcaagggcg aaatcactca ggacggcggc gatatctcga ttccgaaaaa
     6361 ctggcggctc gcttccgttt cccaagaaac gcccgatttg gatatttccg ctttggatta
     6421 cgttttgcag ggcgatgccg agttgcaggc ttttcagacg gcattgaggc aggcggaagt
     6481 gcaaaatgac ggcatgaagc aggcggaata tcatgccaaa ttggaagaaa tcgacgccta
     6541 taccgcgccg gcccgcgcgg caaaattgtt gaacgggctg ggtttttcgc aagaagaaca
     6601 cagccgtccc gtcaaatcct tttccggcgg ctggcgtatg cgcctgaatc ttgcgcaagc
     6661 cctgatttgc cgcgctgatt tgctcttgct tgacgaaccg accaaccatt tggatttgga
     6721 aactgtcttg tggttggaaa accaccttgc ctctttaccc tgcacgcaaa tcatcatttc
     6781 ccacgaccgt gatttcctca atgcggccac cacccaaacc attgaattgt cgcagcaaaa
     6841 gctcacgcaa tacagcggca attacgattt ttaccaaacc gaacgcgcgc agcgtctggc
     6901 gcaacaacaa gccgcatatg tcaaacagca ggcgcaaatc aaacatctgc aatcctttat
     6961 cgaccgcttc aaagccaaag ccactaaagc cgttcaagct caaagccgca tgaaggcgtt
     7021 ggcgaagctc gaacgcatcg ctcccgcaca tctggacagc gagttttcct ttgagtttta
     7081 caatcccgac catctgccca atcctttgct gaagctggag cacgctgatt tgggttacga
     7141 aggtaaaacc gtcctgcatg acattaccct gtcgctggaa agcggcgcac gctacgggct
     7201 attgggtgtc aacggcagcg gtaaatctac gtttatcaaa gctttggcag gaaaaatcga
     7261 tttactctcc ggcagcatcg tccattccga aaaacttaat atcggctatt tcgcccaaca
     7321 ccagcttgac accatccgcg ccgaccaaag ccctgtttgg catatacagc agctttctcc
     7381 cgaagtacgc gaacaagaaa tccgaaattt cctcggcggc ttcgattttg tcggcgatat
     7441 ggcgttacag aaaaccgaac ctttttccgg cggagaaaaa gcccgccttg cccttgccat
     7501 gattatctgg caaaagccga acctgctgct gcttgacgaa ccgaccaacc atctggattt
     7561 ggatatgcgt catgccttaa cgctcgcgtt gcaaagtttc caaggtgcat taatcgtcgt
     7621 atcgcacgac cgcagcctgc ttgaagccac gaccgacagt ttcctcttga tagacaaagg
     7681 tcgtctgaaa aactttgacg gcgatttgaa cgattaccgc caatggcgct tggcacagga
     7741 aaacgccgca accgcgccag ccgcttccac tcaaagccaa aaccgcaagg ataccaagcg
     7801 tatcgaagcg caaatccgtc aagaaaaagc ccgacgcagt aagccgatac aacagaaaat
     7861 cgacaaagcc gaaaaagaaa tggtgcagct ttctgaaatt cagacgggat gcgaagcatt
     7921 tttaacacaa gaagacgctt accttgaagc aaacaaagaa aaattgcaaa ataccttatc
     7981 cgagctggca aaagtcaaaa cacagcttgc ccaaatcgaa gaaacttggc tggcttgtca
     8041 agaagaattg gaacggattg aaactgaaat cgagaaacag tttgccgagc gataaagaag
     8101 cgcgggcagg gtgcttatct ttgcctgcca ataacggtat aattcggacg ttaccaccgc
     8161 ctttttactt taccggtata aacatcagac tttttgccgc cttgggtttc ctgtcccttt
     8221 ccggcgcggc ggcgcaagcc tccatatacc attgcaactc aaatggcaaa agcgtatata
     8281 catcagaccc gtcaggatcg tgtgccgatg ctgatttacc taaaatcagc agccatcagg
     8341 gaggcggata ccgcctgaaa attaaaaaac ttagtcaaga agccaaaata cacacaggaa
     8401 acaaaaagaa aaacaaaaaa catgccggga aaaagaacag acaggctgcc aaagccccga
     8461 aggaaaatca aaaataaaca accgaaaaga aaagcccata aaacgccaag aaaaccttac
     8521 aaaaaatcct caaaaaatca aattattatc cgaatatcaa acacattatg aaatcaaaac
     8581 tccccttaat cctaatcaac ctttccctga tttcaagccc attgggtgcg aatgcggcca
     8641 aaatctatac ctgcacaatc aacggagaaa ccgtttacac caccaagccg tctaaaagct
     8701 gccactcaac cgatttgccc ccaatcggca actacagcag cgaacgctat atcccgcccc
     8761 aaactcccga accggcacca tcaccgtcaa acggcggaca ggctgtcaaa tataaagccc
     8821 cggtcaaaac agtatccaag ccggcaaaat ccaatacgcc gcctcaacaa gcacctgtaa
     8881 ataacagcag acgctccatt ctcgaagcag aattaagcaa tgaacgcaaa gccctgactg
     8941 aagcccaaaa aatgttatca caagcacgtc tggcaaaagg cggcaacatc aaccatcaaa
     9001 aaatcaacgc attgcaaagc aatgttttgg acagacagca aaatatccaa gcactgcaaa
     9061 gagaattggg acgtatgtaa ggtcgtgttt tcaaatcgac cgttccaagg atttgacaga
     9121 agaaatgatg aaaaagcagg agaatttttg ggataagttg ggcgatttac tgtttgcgcc
     9181 cgttgatata atgttttgga ttaaaaaagt atgggcggca tatcctgtgt gcctgctgcc
     9241 tgtatatcgt attgaaggtc aacgcaacac atacagctgt ttccagtagt tcgcataatg
     9301 tatattatgt taaatttata atggattgaa tagatatttt tacaatctca ttcaaaactc
     9361 agacacttaa aaaatttaat cagaacaaat aaaaaagcaa acaaaaatat ccatctttca
     9421 aactcatatt ttgcaataac ctagataagc aagaacagag gcagaacgga ttcgcctttg
     9481 atgtggtggt aggcaaccat tccaatatgt cccagcgaca aaaacagcaa tgaaaatgcc
     9541 agcgtgccgt gaattcctcc gccattgccg atttgacggg caatgcctgc ggcggaacag
     9601 cgagcatcag ggcatacgcc caatgcccaa tttggtgacg gcggtgttgg cggggcggtt
     9661 tttacgttgg cggattccct accctacgca ccaaacagac aatatcagca gcaggattct
     9721 gactgcacgg atatgttggt atgaatcgtc cgcccaagcg gaaaaggctg tcgcgcccaa
     9781 aacagtggca agaataaatt gcttgaattt catgatgttg cttcttttgc acaaagcgtt
     9841 cggaatagtg aagcccgcag cctatggctt tgcggcagtc gcagctttac aagcggtatt
     9901 gcaggaaacc ttaaaacacg atcattgcat caagagctgt tcgaattccg ccaaatgaac
     9961 ctgacacgca aactttgacg cttgaatacg gcattcatag cttccagttt atcggttcga
    10021 taccgtgttg actcaaatag ctgtttgcct gtgaaaaatg gcggcagcca aaaaaaccgc
    10081 gatatgccga cagaggagac ggatgaggcg cggtcagtat caaatgattt tgactgtcta
    10141 tcagcctccc tttttgttgt gcatacccac cccacaacat gaagacaagg tgcttgcggt
    10201 gtgtcgcaag ctgcctgatg acggtatcgg taaagcgttc ccagcctaaa agggcgtgcg
    10261 aatgcgcctg tcctgcacgc accgtcaaaa ccgtgttcag aagcaatatg ccctgctccg
    10321 cccacgctgt caggcagccg tgcgcgggaa tggaaaagcc ttcgatgtcg gtttccaatt
    10381 ctttgtaaat attgagtaaa gacggcggta tgcggacacc ctgccggacg gaaaatgcca
    10441 aaccgtgcgc ctgccctacc ccgtgatacg gatcttgtcc gagaatgacg actttgaccc
    10501 ggtcgaacgc tgtcagccgg aatgcgttga acacatcggc ctccggcgga tagataattt
    10561 gccccgacaa acgttcctgc ctgaccgcat ttaaaatttc ctgaaaatac ggctgctgtt
    10621 tttcgccgcc gagtgcatcg tgccaagtgt ccatatctgc cctatccttt caaattcggc
    10681 tatactgctg atattgcgtt attttgtcaa acgacgcgct ttgtggggaa atgtatgact
    10741 gtatggtttg ttgccgctgt tgccgtctta atcatcgaat tattgacggg aacggtttat
    10801 cttttggttg tcagcgcggc tttggcgggt tcgggcattg cctacgggct gactggcagc
    10861 acgcctgccg ccgtcttgac cgccgcactg ctttccgcgc tgggcatttg gttcgtacat
    10921 gccaaaaccg ccgtgggaaa agttgaaacg gattcatatc aggatttgga taccggaaaa
    10981 tatgccgaaa tcctccgata cacaggcggc aaccgttacg aagtttttta tcgcggtacg
    11041 cactggcagg cgcaaaatac ggggcaggaa gtgtttgaac cgggaacgcg cgccctcatc
    11101 gtccgcaaag aaggtaacct tcttatcatc gcaaaccctt aacactcgga ggaattatgg
    11161 aatttttcat tatcttgttg gcagccgtcg ccgttttcgg cttcaaatcc tttgtcgtca
    11221 tcccccagca ggaagtccac gttgtcgaaa ggctcgggcg tttccatcgc gccctgacgg
    11281 ccggtttgaa tattttgatt ccctttatcg accgcgtcgc ctaccgccat tcgctgaaag
    11341 aaatcccttt agacgtaccc agccaggtct gcatcacgcg cgataatacg caattgactg
    11401 ttgacggcat catctatttc caagtaaccg atcccaaact cgcctcatac ggttcgagca
    11461 actacattat ggcaattacc cagcttgccc aaacgacgct gcgttccgtt atcgggcgta
    11521 tggagttgga caaaacgttt gaagaacgcg acgaaatcaa cagtaccgtc gtctccgccc
    11581 tcgatgaagc cgccggggct tggggtgtga aagtcctccg ttacgaaatc aaggatttgg
    11641 ttccgccgca agaaatcctt cgcgcaatgc aggcacaaat taccgccgaa cgcgaaaaac
    11701 gcgcccgtat tgccgaatcc gaaggccgta aaatcgaaca aatcaacctt gccagtggtc
    11761 agcgtgaagc cgaaatccaa caatccgaag gcgaggctca ggctgcggtc aatgcgtcca
    11821 atgccgagaa aatcgcccgc atcaaccgcg ccaaaggcga agcggaatcc ctgcgccttg
    11881 ttgccgaagc caatgccgaa gccatccgtc aaattgccgc cgcccttcaa acccaaggcg
    11941 gggcggatgc ggtcaatctg aagattgcgg aacaatacgt agccgcgttc aacaatcttg
    12001 ccaaagaaag caatacgctg attatgcccg ccaatgttgc cgacatcggc agcctaattt
    12061 ctgccggcat gaaaattatc gacagcagca aaaccgccaa ataagccgtg atgaaaatac
    12121 cgtctgaagc tctgttttcg ggtttcagac ggtattttta tttaaacccg aacagggttg
    12181 tattccgctt caaatatgtt tcggaaccgt ctacctgccc cggtaaaaac tgccggcaaa
    12241 atatttctgc gcgccctcgc gaagcaagac caaggttacg gcggccaaag gcaatccggc
    12301 caacattccg acaaagccca tcagctctcc gaacgccatc agcgaaaaga taacccaaaa
    12361 cggcgacagg ccgatacggt ctcctacaat tttcggcgta atgaaaaaac tttcgagaaa
    12421 ctgaccgacg gcaaaaaccg cccaaacagc caagattccg ttccacgaac cgaactggag
    12481 caaggctgca acagtggcaa gcagcaatcc cgtaaacgca cccaaatagg ggacaaacac
    12541 caaaataccg gcaaccatac cgatggcaaa tcccgaatcc agtccgacta gcatcaatcc
    12601 caaaccgtaa accaagccca taatcagcat caccagaagc tgaccgcgca aaaattcgcc
    12661 caatacctcg ttcaaattac ccgtaatgcg cgtataagca ccggcaaaac gcctcggaac
    12721 cagtttggcg atgccgcacg accaccgctg ccaatccagc aggaaatagt aaagcagcaa
    12781 gggcggcagc agcaggttgc cgatactgct gacaatattg ccgccctgtt tcatcaaaac
    12841 gggaaaccac gccttaagcg cgttgctcaa ctcgcccgta tgcgcctgaa accacgcaat
    12901 aatagatgcc tgatcgattt ccacatatcc gccgattgta tttttcaacc acggcagcag
    12961 cgtgttctgt ataaaaccga ttaattgggg caggcgagat gccaaattat tgaactgccc
    13021 gaccagcata gggacaataa tcaacaataa tgccaacaac aaaatcaagg aaaacaccat
    13081 cacagacatc gaagcggatg cacggttcaa accctttttc tgcaaccatt cgaccaaagg
    13141 gtccaacaca tacgccagca ccgccgcaac cgcaaacgga gtcaaagtat cgccgagcgc
    13201 gtaaaccagc cagaccaagg cggcaaacgc cgcgccggca cccatccacg gcttgatgcc
    13261 ccgtcctttt ctccgataca taaaatccac ccttccgaaa ataatattgc gcgccagtat
    13321 agcagaaccg ccgcgccgtc aaaaaagcct ccgccttccc gatagcctgt tatcgtggtt
    13381 taggctaaaa tactgcacat ccgatgccct ctgaaaggca aacccgcctt cagacggcat
    13441 accgtacacg ataaggcagc acatcatgac cgaacaaaaa aacaaagcat ccctcatcga
    13501 atttccctgc gccttcccat tgaaagtgat gggcgcggtg catcccgagt tcgagcaggc
    13561 ggtattggac accgtccgcc ttcacgcccc cgacacgcag gcgcaccaca tcaccacgcg
    13621 tccgagcagc aaaggcaact ataccggtgc caccgttcag gtaaaggttg aaaaccaaga
    13681 acaattggac aacatctacc gcgcgctgac ttcgcacgaa ctggtcaaag tggtactttg
    13741 agatgaaaat catacacaaa ggtttggtcg aatatctgcc gacttttgaa gcgatgaaaa
    13801 cctttaatgc cggacggaat gcagacaccg aagacgaatt gtgggtcgtc gaacacccgc
    13861 ccgtctttac gcaaggactg gcgggaaaac ccgagcacct gctgattagg gacgacatcc
    13921 ccgtcgtcca aatcgaccgg ggcgggcaga ttacctatca cgggcccggg caattggtcg
    13981 tttatacgat gattgatttc aaacggcgca aaaccagcgt ccgcaacatc gtttccgcgc
    14041 ttgaaaacag catcatcgcc acattggcgg aatacggcat cgaagcggcg gcagatccca
    14101 aacgccccgg catttacgtc ggcgaacgca aaatcgcctc actggggctg cgtatcaaaa
    14161 acggttccgt ctatcacgga ctcgcactca acgtcaatat ggatttaagc ccgtttaccc
    14221 aaatcaaccc ctgcggctac gccggtatgg aaatgacgca aatcgcggat tttgtccaac
    14281 cctgccccgc gccggacgaa gtcgcctcca aactcaccgc acaccttgaa acacaactca
    14341 caccgaaagc agacaacaat gagtgaaatc aaaaccgacg accccaaacg cggcatcaaa
    14401 ctcagaggcg cggacaaaac cgcccgcatc cccatcaaag tcgtccccct tcaggaaaaa
    14461 ctgaaaaagc ccgaatggat acgcgccaaa ctcccatcgc gcaaattctt tgaaatcaaa
    14521 gacattttgc gcgaacaaaa gatgcacacc gtttgcgagg aagcctcctg ccccaacatc
    14581 ggcgaatgct tcagcaaagg cacggcgacc ttcatgatta tgggcgacat ctgcacccgc
    14641 cgctgcccgt tctgcgacgt gggacacggc cgtccgaata tgctcgaccc cgacgagccg
    14701 aaaaacctcg cagaatccgt caaagccatg aacctgcgtt acgtcgtcat cacctcggtt
    14761 gaccgcgacg acctgcgcga cggcggcgcc caacacttcg ccgactgcat caaagccatc
    14821 cgcgaaacca gcccgaacac caaaatcgaa atcctcgtcc ccgacttccg aggccgcttg
    14881 gacatcgcac tgaaaatcct tgccgaaacc ccgcccgacg tgatgaacca caacttggaa
    14941 acccatccga gcctgtacag aaaagcccgt cccggtgcca attatcaaca ttctttagac
    15001 ttattaaaac gttataaaga aatgatgccg catatcccga ccaaatccgg cattatggta
    15061 ggcttgggcg aaacagacga agacgtgcgt gaaattatgc gcgatatgcg ggcgcacaat
    15121 atcgagatga ttaccatcgg acagtacctc cagccttcag acggacactt gcctgtcttg
    15181 cgctacgtta cacccgaaca attcaaaata tttgaaaaag aagcatacga actgggcttt
    15241 accaatgccg ccatcggcgc gatggtacgt tcaagttacc atgccgacga acaggcggcg
    15301 gaagccttac gcgaaagcca cggcggttgc gggcatcatt aaactcggaa caccgttccg
    15361 aagtaaaaaa taatttccta cgctgctgtc tgccgccttg tttgatgtaa acaacatcaa
    15421 tttgaattgt ccatgccgtc tgaaagtcct tcagacggca taatttattc ccaaaatccg
    15481 ataatctcaa aagaatctgt cggttaatga gaaagattca ttataaattt tgataaaaaa
    15541 ccgtgctcta tttgtttttt gctatggtta acaagtgtaa atgttcttta ttgatgagat
    15601 ggattcttag ttttagcagc aaaaaaaacc gataagaaac agcatactga tttggaaggc
    15661 atcggggaat acgccctatt tattttgata attgtttgta ttttaaatag aaaatttgtc
    15721 atgccgacag cttttgcata caatgcacgc atcccgaaca gcgttacaga acgccgttta
    15781 accgcacgaa acccaaatta agaaaagaga gatattatgc aaggcaatca agctgttgtt
    15841 gattacatga acgaattgct gtccggcgag ctggcggcac gcgaccaata cttcatccac
    15901 tcccgcctct actccgaatg gggctacacc aaactttttg aacgtctcaa tcacgagatg
    15961 gaagaagaaa ccacacacgc cgaagacttc atccgacgca tcctgatgct gggcggtacg
    16021 ccgaaaatgg cacgcgccga actgaatatc ggcacggacg tggtttcctg cctcaaagcc
    16081 gacctgcaaa ccgaatacga agtgcgcgac gctttgaaaa aaggcatcaa actgtgcgaa
    16141 gaggctcaag actatgttac gcgcgacctg atggttgccc aactgaaaga caccgaagaa
    16201 gaccacgcac actggttgga acagcagctg cgcctgatcg agctgattgg cgaaggcaac
    16261 tactaccaaa gccaactgta atccgtatta ggaataaaag gagtccgtat gaaaggcgac
    16321 cgtttggtta tccgcgagct gaacaaaaac ttaggcttgc tgctggtaac cattaaccaa
    16381 tatttccttc acgcccgtat tttgaaaaac tggggctttg aagaactggg cgaacatttc
    16441 ttcaaacaat caatcgtaga aatgaaagct gccgacgatt tgattgaacg catcctcttc
    16501 cttgagggat tgcccaattt gcaagagctg gccaagctgt tgattggtga atctaccgaa
    16561 gaaatcatcg cctgcgattt gaccaaagaa caggaaaaac acgaagccct gcttgccgcc
    16621 atcgccacag cagaagcgca acaggattat gtcagccgtg atttgttgga aaaacaaaaa
    16681 gatactaacg aagaacacat cgactggctc gaaacccagc aggaactgat cggcaaaatc
    16741 ggtttgccga actacctgca aacagcggcg caagaggact aaaacacaaa ccactgccaa
    16801 atacagcagt cccctgcatc atcagacggc atatccgtat aaacgggtat gccgtttttc
    16861 tttgccggtt tacgaataaa atctaaattg atttgaatca atactcgata acggtttttg
    16921 tttgataata ccgatatgaa atctagattg tttgtttgct gtttcccaat ccgtcttgat
    16981 tttacctctt tcctcttgat gtgtgtgtgt gtttgggtgt ggctgccgcc accccttttt
    17041 tttggctttt atgtgaagta aaatccgtaa cagcaaatgc ttaactttta accatttatc
    17101 cggaaacaaa tgatgggcaa caaattgact ctgcctgccg aattgcctga cgaacaggat
    17161 ttgcgggcgg tattggcata caatatgcgg cttttccgcg tgaacaaggg ttggtcgcaa
    17221 gaggaattgg cgcggcaatg cggtttggac aggacttatg tgtcggcagt cgagcgcaaa
    17281 cgctggaaca ttgccctgtc gaatatcgaa aaaatggcgg cggctttggg cgtggcggcg
    17341 tatcagttgc tgctgccgcc gcaggaacgg ttgaacctga tgaccaattc cgccgatacc
    17401 cgacaagtgc cgtctgaaag cggtatttga ccgccgaacc atccgcctcc ctttttcccg
    17461 ccaatcgaaa gaataaaaat gcctgaaaac ctgtcttcag cactggaaac cttcaaacag
    17521 cagcgcaatg ccgccgaagc gcattatttg aaagccaacc gcgtgtcggt atttttcaga
    17581 gaatacacgg cggcagtcga aaccttgctg gcggcattgt gggcagaaca ttttcaaaac
    17641 agcgcgttat gcctgatggc ggtaggcggc ttcgggcgcg gcgaaccgta tccctgttcg
    17701 gatgtggatt tggcggttgt ctcccctgcc ccgctttcag acggcattca ggaacagatt
    17761 gcccgattta tccaaactct gtgggattgc aaactaatgc cgtctgtaaa aagcggcagc
    17821 gttgacgaac tgtgcgaaag cgtgcgcgac gatattacgg gggacacagc gtttttagag
    17881 gctaggtttt tgtttggcaa ccgccaaaca gcagacgaat tggcggaaaa aatgaacgtg
    17941 caacgcaatg tagcggcatt tatcgaagca aaattggtgg agatggaaca ccgccacgcc
    18001 aaatcgcaag gttcgggggc ggtgttggag ccgaatatca aaagctgtcc gggcggtctg
    18061 cgcgacatcc acaccctgct ttggatagcg aaggcgcaag gcttggcggc caacctgccc
    18121 gacctgctca aacagcggat tttgacacgt gccgaagccg gtatgctttc gcacggctac
    18181 cgccgcctcg cccacatccg catccggctg catttaaacg ccaagcgcgc cgaagaccgc
    18241 ctgcttttcg atttgcagcc gcaagttgcc gaaagtatgg gttatcaaga tgaaaaccgc
    18301 cgccgtcaaa gcgaagaact gatgcgcgtg ttttaccgtg cggtcaaaac cgtcaaacaa
    18361 ctgggcggca tcctcacgcc catgctgcga agccgcgttt cctccacgcc gatgcgcgtt
    18421 accctgcgga ttgacgacga ctacatccaa gtcaacaacc aaatcgccgc gcggcacacc
    18481 gatatttttt tcagacggcc cgaacacatt ttcaaaatcg tcgaaatcat gcagcagcgc
    18541 aacgacatta ctgcgctcga accgcaaacc ctgcgcgcat ggtggggggc gacgcgcaaa
    18601 atcaaccgca gcttctacca aaattctgaa aaccgtcgcc gcttcgccgg ttttttccgc
    18661 agcggcaacg gactgaccca aaccctgcgc tttctcaacc tctacggcgt gttgggccgc
    18721 tatctgcccg cgtgggaaaa aatcgtcggc ctgcttcaac acgacttgtt ccacatctat
    18781 cccgtggacg accacatcct tgccgtcgtc cgcaacgtcc gccgccttgc cctggatatg
    18841 cacagccatg agctgcccta cgcctccgcg ctgatgcagt cctttgaaaa acaagacatc
    18901 ctctaccttg ccgccttctt ccacgacatc gccaaaggac gcggcggcga ccatgccgta
    18961 caaggcatcg cagacgcgcg ccaatttgcc gccgaccact tcctgaccga agaagaaagc
    19021 gacctgctcg cctggctggt tgaaaaccac ctccttatgt ctgccgtcgc ccaaaaagaa
    19081 gacatccaag accccggcgt actcgatgcc ttctgcaaac gcgtccaaac ccacgaacgc
    19141 ctcagcgcgc tctacctcct gaccatttcc gacatacgcg gcaccaatcc caagctgtgg
    19201 aacgcctggc gcgccagcct gctggaaagc ctgttccacg ccgccggacg ctgcctcgca
    19261 ggcaacgacg gcaacccgca cgccctcttc ggccgccgcc ggcaggaagc cgccgactta
    19321 cttacccgcg ccgccgtccc cgaaaaacag cagaaaaaac tatggaacgc gctcggttcc
    19381 gcctatttcg cccgccatca gtcccgcgaa atcctgtggc acgccgccaa cctcgtacac
    19441 gactttgaac cccccatcgt ccgcagccgc atcctacccc aaagcgacag ctttcaagtg
    19501 atggtcttta tgcccaacgg cccgcgcctg ttcgcccgcc tctgccgcat cttcagccgc
    19561 cacggcttcg atatcctcgc cgcccgcgcc tttatcaccg aacacgacta catcctcgac
    19621 accttcatcg tgcaaatccc ctcgcagcac gcccccgaag actaccccga catccaaagc
    19681 gcgctcgaag ccgaactcaa cagctttatc cacggacaca ccgttgccga aacccaaagc
    19741 tgcaaccgcc gcatcagccg ccgcagccgc tatatgccta ttgcgccaag catcaccatc
    19801 acccccgaag aagactatcc ggaccggtat tccgtcgaaa tcaccgccgt caaccgcccc
    19861 ttcctgctcg ccgacatggc ggaagtcttc ttcgcccaca acgtcagcct gcgctacgcc
    19921 aaaatctcca cgttggacga acgcgtcgaa gacagcttta ccgtcttcag ccccgacttg
    19981 aaaaacccaa aaatccagtc ctcattgaaa caggctttgc tggaacagtt ggcataagca
    20041 ccctgccggt tttcaaacgg catcccgccg ccacgccgta tgccgtctga aggcggggga
    20101 tattgcaaac aatataaact gtcaagccgc cggcaacgtg ctataatgcc gtgcatcttg
    20161 cacaaaccga aagaccgagc attatgcgta tcgtagaaaa agcctatact ttcgacgatg
    20221 ttttgttggt tcccgcacat tcgaccgtgc tgccgcgaga cgttaaactt caaaccaagc
    20281 ttacccgcga aatcacactc aacctccccc tgctttccgc cgcgatggat accgttaccg
    20341 aggcgcgcct cgccatttcg atggcacagg aaggcggcat cggcatcatc cataaaaaca
    20401 tgccgcccga aatgcaggcg cgcgccgttt ccaaagtaaa acgccacgaa agcggcgtgg
    20461 tcaaagaccc cgtaaccgtt gcgccgacaa cgctcatccg cgaagtcttg gaaatgcgcg
    20521 cgcagcgcaa acgcaaaatg tccggcctgc ccgtcgttga aaacggcaaa gtcgtcggca
    20581 tcgtaaccaa ccgcgacctg cgttttgaaa accgcgtcga tttgcccgtt tccgccatta
    20641 tgaccccgcg cgaacgtctg gttaccgtcc ccgaaggcac aagcatagac gaagcgcgcg
    20701 aactgatgca cacgcacaaa gtcgaacgcg ttttggtcct gaacgaaaaa gacgagctta
    20761 aaggcttgat taccgttaaa gatattttaa aaaccaccga gtttcccaat gccaacaaag
    20821 actccgaagg ccgtctgcgc gtcggtgcgg cagtcggcac cggcggcgac accgacgagc
    20881 gcgtcaaagc cttggtcgaa gccggcgcgg acgtgattgt cgtcgatact gcccacggtc
    20941 atagccaagg cgtgatcgac cgcgtgcgtt gggtcaaaga aacctatccg cacatccaag
    21001 tcatcggcgg caacatcgcc actgccaaag ccgcattgga tttggtcacc gtcggcgcgg
    21061 atgccgtcaa agtcggtatc ggtccgggat cgatttgcac cacccgtatc gtggcaggcg
    21121 tgggcgtgcc gcaactgacc gccattcaca acgttgccga agccctcaaa ggcacgggcg
    21181 ttccgctgat tgccgacggc ggcatccgct tctccggcga catcgccaaa gccctcgccg
    21241 caggcgcgta cagcgtcatg ctcggcggta tgtttgccgg cacggaagaa gccccgggcg
    21301 aaatcgaact ctaccaaggc cgctcgtaca aatcctatcg cggtatgggt tccttgggcg
    21361 cgatgagcca aggttctgcc gaccgctact tccaagacaa aaccgacagc accgacaaat
    21421 acgtccccga aggcatcgaa ggccgcgttc cctacaaagg cccgattgtg aacatcatcc
    21481 accaactgac cggcggactg cgttccagta tgggttattt gggttgcgcc aatattgccg
    21541 aaatgcacga aaaagccgaa tttgtggaaa tcacttccgc aggtatgagc gaatcgcacg
    21601 ttcacgacgt tcaaattacc aaagaagcgc cgaattacca ccgctgattt gaacagcctt
    21661 tcaggacaaa tgccgtctga aacctaattt ccgagtttca gacggcattt ttgttcgttc
    21721 aaggcaagcc gcacgggctg attgcgcgga cgggcaaagc cattctgacg gacacgccga
    21781 aagaatgaaa atgccgtctg aacgccgttt gcaccccgtc tgccgaatac cgtcggcctt
    21841 taaaacggcg gtggaaataa acgcaatctt tcaatacggg cgtttcgatt ggtcggaaat
    21901 gatgcgccgg ccgccatatc aaaaaatcgg gcggcggctt cgggaaagta tgccccgccc
    21961 tccccaagcc gccccgtcag cattatgatt tgcctttccg ttttttgacc ttaatcggaa
    22021 ccggtttctt tgcctttttc cccgattcgg caacggcagg cacgcccctt ccccttactt
    22081 tgccgcatcg ggctgttttt ttctcggcgg tggttttcga tttccctttc cccgccgccc
    22141 ctgccggttt ggcagatgcg gataatttga ccttccgccg cctgccgctt cctccggcaa
    22201 ttaggacaaa gtcgattttt ccatcatcca aatcggcacg cgcgacccgg acggcaaccc
    22261 tgtcccccat attgaaacgg atgccgctgc gttcgccttc gattgccatg atttcggggc
    22321 ggaagttgaa ataatcttcg cccaaatcgc tgatatgtac cagaccgtcg atatggatat
    22381 cgtccaaagt gacaaatatt ccaaaatttg ccaccccgga gattttgcct tcaaatattt
    22441 caccgacctt atcgcgcata taataagttt tcagccagtt ttccacatcg cggccagcat
    22501 cgtcggcacg gcgttcgcaa aacgaagtat gcacgcccaa agcctgccag cttttgtttg
    22561 gcgtgtaggt tttccggttc aatacggctt tgatggcacg gtggacggtc aggtcgggat
    22621 agcggcggat gggcgaggta aagtgggcgt atgcttcata agccaaaccg aaatgccctt
    22681 cgcaatgcgg ttcgtaaacc gcctgctgca tggagcgcaa catcatgact tgcagcaatt
    22741 cggcatccgg cctgcctttg aattgttcgg caagcgcggc atagtctttc ggcgacgggt
    22801 tgtcgccgcc gccaagttga agccccaaca gaccgagctg ctcgcgcagg gtggcgagtt
    22861 tttcggacgt ggggcccaaa tggttgcgga acaatgcggt atgcttgttt ttcaacagaa
    22921 aatccgccgc gcaaacattc gccgccaaca tacattcttc aatcagctta tgggcatcgt
    22981 tgcggacaac gggcacgatt ttttcgattt tgccgttgcc gtcgaaaagc atttgcgttt
    23041 cgacgctttc aaactcgacc gcgccgcgcg ccagacgttt tttctgcaaa atcttaaaca
    23101 gcttgtaaag cgtgtcgatt tgggctttgt gcggattccc gatgccgtct gaaagccatt
    23161 tccaaacttg gttgtaggtc aggcgggcat gagagcgcat cacggcggga tagaagcggt
    23221 attctttgat attgcccgca taggtaacga ccatatcgca caccatacac aaacgctcga
    23281 catcgggatt gagcgagcag atgccgttgg acaggttttc cggcagcatc ggaatcatac
    23341 ggcgcgggaa atacacgctg gtactgcgtt cttgagcatc tgcatcaatc gcatcgtcag
    23401 ggcggacata atggctgaca tccgcaatcg ccacgaccag acggtaattg cgtccgactt
    23461 tttcggcaaa caccgcgtcg tcgaaatcgc gcgccgtttc gccgtctatc gttaccaaag
    23521 gaaggtcgca caaatcgacg cggcctttca aatcgctttt gcgtacatgg tcgggaattt
    23581 ttttcgcgga tttggcacac gcttcactga atcggtgcgg caaatgatgc ttgcgcacgg
    23641 caatttcgat ttccatcccg ctgtcggcat aatcgcccaa aacttcaatg atttttgcca
    23701 ctgcaggccg gttttgctcg ggataaacct caattttgcc gacgataacc tgaccggatt
    23761 cgggtttgaa acgcgccacg ccgtccggtt ccaacacgat gctttggttc agacgcttgt
    23821 cttccggctc caaaatcgcc acgcccctat ccatatagaa acggccgaca actttgcttt
    23881 gcgcgcgttc gacaatatcc agaaccgtcc cttcgcggcg gcccctgcgg tcgatacccg
    23941 caggacggac ggtaacggtg tcgccgtgca tgacaccacg catctggcgt tcgtataaaa
    24001 cgaaatcccc ttcgtccatc ggcatgagcg gcacggcgaa gccgaaaccg tccttatgcg
    24061 cctcgacgcg gcatttgacc aaatccagct tgtccgccgc gcaaactgcg cctcggcggt
    24121 tgattaaaac ctgaccgtcc cgcgccatcg ccttcagacg gcgttcaaaa aagacatact
    24181 cgtcttccgt aatcgacagc tcgcgtgcaa gcgattcgat ttttgaaggc acacctttgc
    24241 gctccaacaa ttcgattatc cattcccgac tgggcaaagg atgttcataa cgctgttttt
    24301 cacgacttaa aaacgggtct ttttcccgta aatttaaaga tttaatattt ttattcattt
    24361 tgactgttga cattctcttt atgaactata taatagcgac ttctttgcgg caggacactg
    24421 taccgcaaaa cagcaaataa agcaaagccc gggtggcgga attggtagac gcgccagctt
    24481 caggtgctgg tatcctcacg ggtgtggaag ttcgagtctt ctcccgggca ccaaataaca
    24541 aattatcccg attaggataa tttccgccat tagagaggtg gatgagtggt ttaagtcgca
    24601 cgcctggaaa gcgtgtatac gtgaatagcg tatcgagggt tcgaatccct tcctctctgc
    24661 caagtacaaa tcccaaactt ttacagtttg ggattttttg ttacttacgt attgatttta
    24721 ttgcgtaaaa tcagccacaa cctattaccc tacgttacca ttgattacta tccgttaccg
    24781 tttttttatg gtatgttttg cggtatgttt tatcctcttc ctaagcctgt aaggactcaa
    24841 aaggcgtatc ccttcaaacg atattgttcg acatgctgcc gggaaaccga acccgatacc
    24901 gaaacgcgac ggggcgatat gccgctggaa aaatggcggc agtttcttga ttggatagga
    24961 aaaacgccgt ctgaaacagc ttcaagaagc cgcaaagccc gtccgaataa aaaataccgt
    25021 ctgaaatttt tcagacggca tcgcgtatca tttaaacatt aatccagctt tttaaaatga
    25081 cggcggcgtt ccaattcgct caggtaacgc ttgcgcaggc ggatggattg cggcgtgatt
    25141 tccaccagct cgtcatcgtc gataaactcg accgcgcctt ccagcgtcag tttgatcggc
    25201 gtggtcaggc gcaccgcttc gtcggtaccg ctggcacgga tattggtgag ttttttgcct
    25261 ttgagcgggt tgaccaccaa atcgttgtcg cggctgtgga tgccgataat cataccttcg
    25321 tagattttgt cgttgggcga tacgaacata cggccgcggt cttcaagatt ccacaaggcg
    25381 taagcaaccg cctcgccctg ctcttgggac accagtacgc cgttgtggcg gccgggcata
    25441 tcgggtttga cgggcgcgta gtcgtcgaac acgtggctca tcagcccgac gccgcgcgtc
    25501 agggtcatga attcgccttg gaaaccgatc aagccgcgcg ctggaatatg gtattcgagg
    25561 cgggtgcgtc cgttgccgtc gctttccata ttggtcagtt cgccacggcg gcggccgagt
    25621 tcttccatta ccgcgccttg gttgtcgtcg ggtacgtcca cagtcaggtt ttcataaggt
    25681 tcgcattttt gaccgtcgat gtctcggtac acgacgcgcg gcttgccgac ggcgagttcg
    25741 tagccttcgc ggcgcatatt ttccagcaaa atcgtcaggt gcagttcgcc gcgcccggat
    25801 acgcggaaca cgtcggcatc ggcggtgtct tccacgcgca gggcaacgtt ggtcagcaat
    25861 tctttttgca ggcggtcgcg gatttggcgg ctggtcacga atttgccttc tgtacctgcg
    25921 agcgggctgg tgtttaccat aaagtccatc gtcagcgtcg gttcgtccac tctcaacatc
    25981 ggcaggcctt tggggttgtc tttgtcggtg atggttacgc cgatgccgat gtcttcgata
    26041 ccggaaataa tcacgatgtc gccggcttcg gcttcttcaa gcggcacgcg ttccaagcct
    26101 ttgaaaccca aaagctggtt gatgcggcct tgggcgattt gctgctcgtg gttcatcacg
    26161 gcaacggttt gaccgggttt gatgcgtccg ttcaagatac gaccgatacc gaggcggccg
    26221 gtgtagttgt cgtagtcgag ttgggaaatt tgcagttgca gcggctcgtc cgcgctgccg
    26281 ctcggtgcag gcgtgtattt taggatggtg tcgaacagcg ggcgcatatc gctgctctcg
    26341 tcggtttctt ccagcttggc aaagccgctc aaacctgaag cgtaaacaat cgggaaatcc
    26401 aactgctcgt cggtcgcacc caagttgtcg aacaactcga aagtctggtc gataacccag
    26461 ctcggacggg cggacggttt gtcgattttg ttgatgacga caatcggttt cagccccaaa
    26521 gccaaggctt ttttggtcac gaaacgggtt tgcggcatcg gaccttcctg tgcgtccacc
    26581 aacaagacga cgcaatccac catccccaaa acgcgctcca cttcgccgcc gaagtcggcg
    26641 tgtcccggcg tgtcgacgat attgatgtgg cagccttcgt aatcgatggc ggtgtttttg
    26701 gcgaggatgg tgatgccgcg ttctttttca aggtcgttgc tgtccatcac gcgctcgtca
    26761 acctgctggt tggcgcggaa tgtgccggat tggcgcagca gttggtcgac caatgtggtt
    26821 ttgccgtggt cgacgtgtgc gatgatggcg atgttgcgga tttgtttcat aattaatgtt
    26881 tttcaaaaac tgtaagagat aactgcgtat tataccacga tttaccggac agtatttgga
    26941 aaatgaagat gccgtctgaa gcccttgcct ttcagacggc atcttcttac atactttcag
    27001 ttatccagtt gcgcagccac cttgccgccg gggcgatttc ccctgccgac agcccagccg
    27061 caatgcctgc cgattccttt ataacatccg ccgccgcgcc gtgcagccac gcgcccgcgc
    27121 aggcggcttc aaaaaccggc acgccctgtg cgagcagact gccgatgatg ccgcccaata
    27181 cgtcgccact gcccgccgtt gccaatcccg cattgccgct ttcgttgaca tagatttccg
    27241 tatcggacgc ggcaaccaat gttttgtgcc cctttaaaac cacggttgca ccgagaattg
    27301 cccctatctt ccttactgcc gccgtccgat ccgcctgaac ctgtgcaact gtcgttccaa
    27361 gcaggcgcgc ggcttcggcg gggtgcggcg ttaaaatcag gtttttacac ccgcgcgcca
    27421 gatttcgggt ttcggcatcg gttgataata tgttcagcgc gtccgcatcc aaaacgacgg
    27481 cttcgtctcg attccgcgcc agcactgccg ccaccgtttc cactgccgcc gcatctgtac
    27541 ccaagccgca acccgccgtc caagcgttta tgccttgacg cttggtcaaa ccgtctgccg
    27601 tgtccaacat aatctcggga aaaccggcaa tgacggcaaa aggcagcgta tcctgattga
    27661 aacctgcccg caccttaccg cagccgagat acattgccgc cgatgccgcc aatacgggcg
    27721 cgccgctcat ccctgccgat ccgccgacta cggcgagcgt gccgaaagtc cctttgtgcg
    27781 aatcttccgc acgggctttg aaaacatggg gaaaacgcaa tgccgtctga agcatccggc
    27841 ggcggctttc gcctgaaaga tggaaaacgg gaaacatttc ccctccaatc ggttaaaatt
    27901 aaggctcgaa aacaaaccga ttctaccgcc ggaaaccgat tatgaaaaac aaaaccaaag
    27961 tctgggactt ccccacccgc cttttccact ggctgcttgc cgcatccctg ccctttatgt
    28021 ggtatagcgc aaaagccggc ggcgatatgc tgcaatggca cacgcgcgtc gggctgctcg
    28081 tccttttcct gctcgtattc cgcctctgct ggggcatttg gggcagcgat accgcccgtt
    28141 tctcccgttt cgtccgaggt tgggcaggta tacgcggcta tctgaaaaac ggcattcccg
    28201 aacatatcca gcccggacac aaccccttgg gcgcactgat ggtcgttgcg cttttggccg
    28261 ccgtctcatt tcaagtcggc acggggcttt ttgccgccga tgaaaacacc ttcagcacca
    28321 acggctacct caaccatttg gtttccgaac atacgggcag ccttatacgg aaaatccacc
    28381 tcaacttttt caagctgctc gccgtttttt ccgcagtcca catcgccgcc gtcgccgcat
    28441 accgcatatt caaaaagaaa aacctcgtcc gcccgatgat aaccggcttc aaatacatcg
    28501 aaggcaaaac ctcaatccgc tttgccggca aagccgcgct tgccgccgca ttatcggttg
    28561 ccgcgcttgc cgcagccgcc atcctgctcc tgtcctgaaa cccgacatca tgccgtctga
    28621 agcccttttc tgcctttctg acggcatttt ccccaccgca caaacggtct gaatgcccga
    28681 aacggcttca ggccgttttt ccgtccaaaa ccgttatata gcggattaac aaaaatcagg
    28741 acaaggcggc gggccgcagg cagtacaaat ggtatggaac cggttcgccc ggtgcttggg
    28801 cgccttaggg aaccgttccc tttgagccgg ggcggggcaa cgccgtaccg gtttttgtta
    28861 atccgctata aaacggaaaa ccgccgcatc cgcagccgcc ccccatatag ctttcagctt
    28921 ctaacaaatg cctttttatt atttaaaatg acccgcattc cgacaaaaat gccgcgacgc
    28981 accgtatcgg aaacaaggtg cgggcaaaca gcaaaggagt cagccagtga attattttcc
    29041 cattttcgcc aacctctccg gccgccccgt attggtcgta ggcggcggcg cggtcgccgc
    29101 acgcaaaatc agcctgctgc cgaaggcggg cgcagaggtc ggggttgccg caaaacacct
    29161 gaatgccgaa ctctccgcgt tggcggcgga aaacaaaatc ctgtggcttg ccaaagaatt
    29221 tcgtgccgaa cacatccgca cggttttcct catcattgcg gcaagcagcg accaagccct
    29281 caaccggcgc gttttccatc ttgccgaaag ctgccaaaag ccggtcaacg tggtgggcga
    29341 ccgtgaccat tgcagcttca tcttcccgtc ggttatcgac cgcgaccctg ttcagattgc
    29401 cgtttccagc tccggcagcg cgcccgtcct cgcccgcctg ctgcgtgaaa ggctggaagc
    29461 cttgctgccg ccgtctttgg gcgatatggc ggaaatttca ggaaggtggc gcgatgccgg
    29521 tttttatttc cccgtccaac tggtcgtccg ccaagatgcc gacaaggccg acggtttccg
    29581 aggctatcag gggcgcatcg aacgcggttc ggtcaccgtc gggcaaaccg tccgcatcga
    29641 accgaacggg ctgaccgccg aagtgtccga aatcatcgcg ccgaaagggg aagtggcgca
    29701 ggcttttgcc ggcgaggcgg caaccatccg gctggaccgc gacatcgatg tttcgcgcgg
    29761 cgatcttttt gtcgataaaa atttcccgct cgccccgcaa aaacatccgg aagccacgct
    29821 ttgctggttt gacgaacgcc cgctcaacac cgcgcgcaaa tacctgctca agcacggcac
    29881 gcaaaccgtg ccggcaaaag tcggggaaat tgaaagcgtt ttggatgtcc gcacgctgga
    29941 acaagaggcc ggcgcggaat ccttaaagat gaacgacatc gccaaagtcc gcatcaacct
    30001 tcaaaaaccc gttacggcaa cgccttatgc ggaaaacacc gccgccggtt cgtttatcct
    30061 gatagacgaa gcgacatacg gtactgttgc ggcaggtatg attttatgag tgaacacgat
    30121 atgcagaaca caaatccgcc attaccgcct atgccgcccg aaatcacgca gctcctgtgt
    30181 cggggctgtc cggctacgct tgggcaaaag caggaaacgg ggcatccgca gggctgcccg
    30241 cgcttcagac ggcattgccg gcggcagaac ctttttccgt aaccgtcctt tccgcctcgc
    30301 aaaccggcaa tgcgaaatcc gttgccgaca aagcggcgga cagcctgaaa gccgccggca
    30361 tccaagtcag gcgcgccgaa ctgaaaggct acaaggcgaa aaacatcgcc ggcgaacgcc
    30421 gcctgctgct ggttacgtcc acccagggcg aaggcgaacc gccggaagaa gccgtcgtgc
    30481 tgcacaaact gctgaactgc aaaaaagccc cgaaattgga caaactccaa tttgccgtac
    30541 tgggtttggg cgacagctcc tatccgaatt tctgccgggc gggcaaagat ttcgacaaac
    30601 gttttgaaga attgggcgca aaacgcctgc tcgaacgcgt tgatgcggat ttggactttg
    30661 ccgccgccgc agacggatgg acaggccgca ttgtcgcgcg cttaaaagaa gaagccgcaa
    30721 aaaaccgggc aacgcccgcg ccgcaggcaa cgtccccccg ccggccttca gacggcatcg
    30781 gagggcaggt actgcaaggc agaccccttt cccgccgccc tgctggccaa tcagaaaatc
    30841 accgcccgcc aatccgataa agacgtgcgc cacatcgaaa tcgatttgag cggttcggat
    30901 ttgcactacc tcccgggcga cgcgctcggc gtttggtttg acaacgatcc ggcactggtc
    30961 tgggaaatcc tagacctgct cggcatcaat ccggcaacgg aaatacaggc gggcggaaaa
    31021 accctgccgg ttgcctccgc actgttatcc catttcgaac tcacgcaaaa cacccccgcc
    31081 tttgtcaaag gctatgccac gttcgccgat gatgacgaac tcgaccgtat tgctgccgac
    31141 aacgccgttt tgcaaggctt tgtgcaaagc acgccgattg ccggtgtgct gcaccgcttc
    31201 ccggcaaaac tgacggcgga acaattcgcc ggcctgctgc gcccgcttgc gccgcgcctg
    31261 tattcgattt cctcgtcgca ggcggaagcg ggggacgaag tgcacctgac cgtcggcgca
    31321 gtgcgtttcg aacacgaaag gcgcgccagg tcgggcggcg catcgggttt ctttgccgac
    31381 cggctggaag aggacggcac ggtgcgcgtg tttgcggaac gcaacgacgg cttcaggctg
    31441 cccgaagaca gccgcaagcc gattgtgatg atcggctccg gtaccggcgt cgcaccgttc
    31501 cgcgccttcg tccaacaacg tgccgcagaa aatgcggaag gcagaaactg gctgattttc
    31561 ggcaatccgc attttgccgc cgacttcctc tatcagaccg aatggcagca gtttgccaaa
    31621 gacggcttcc tgcacagata tgacttcgcc tggtcgcgcg atcaggaaga aaaaatctat
    31681 gtgcaggaca aaatccgcga acaggcggaa ggactttggc aatggctgca ggaaggcgcg
    31741 catatctatg tgtgcggcga tgcggcaaaa atggcaaaag aagtggaagc cgccttgctg
    31801 gatgtgatta tcggggcagg gcattcggac gaagacggcg cagaaggata tttggatatg
    31861 ctgcgcgaag aaaaacgcta tcagcgtgat gtttattgat taaatataat cgggaggaac
    31921 acaaaatgac cgtacaggcc aagacaaaag gtttggcgtg gcaagaaaag ccgctatccg
    31981 acaacgaacg tctgaaaacc gaaagcaatt ttttacgcgg cacgattttg gacgatttga
    32041 aagacccgct cacgggcggc ttcaaaggcg acaacttcca actcatccgc ttccacagta
    32101 tgtatgagca ggatggccgc gacatccgca ccgaggcaaa actcgagccc ttgaaattta
    32161 tgcttttgcg ctgccggctg ccggcgggat catcaaaccg tcccagtgga tagaactgga
    32221 caaattcgcc cgggaaaaga gtcattaccg ctccatccgg ctaaccaacc ggcaaacctt
    32281 ccaatttcac ggcgtgccga aaaccaagtt gcagacgata ccccgcctct tgcacagcct
    32341 gggtttggat tccatcgcta cggcggcgga tatgaaccgc aatgtgcttt gcacctccaa
    32401 cccgatcgag tccgagctgc accggcaggc ttacgaatac gcgaaaaaga tttccgaaca
    32461 cctgctgccg cgcacgcgcg gttatctgga tatgtgggtg gacggcaaaa aagttcaaag
    32521 ttccgacgac ttccttcaag aaggcgagcc gattttgggc aaaacctatc tgccgcgaaa
    32581 attcaaaacc gcagtcgtca tcccgccctt gaacgatgtg gactgctacg gtaacgattt
    32641 ggatttcgtc gccgtttcag acggcaacgg acagcttgcc ggcttcaatg ttttggcggg
    32701 cggcgggctt tcgacggaac acggcaacgg caacaccaaa acctatccga acatttcact
    32761 ggaactgggt ttcgtgcctc cggaacacgc actgaaggcc gccgaagcgg tggtaaccac
    32821 gcagcgcaac ttcggcaacc gcagccaccg caaaaacgac cgcacccgct acaccattca
    32881 aaatatgggc ttggacaact tccgcacgga agtcgaacgc cgtatgggta tgccgttcaa
    32941 acccatacgg ccgttcaagt ttaccgggcg cggcgaccgc atcggccggg tgaaaggcat
    33001 agacggcaac tggcatttga cccttttcat cgaaagcggg cgtttggttg acgaaggcgg
    33061 gaaacagctt ctgaccggcg tgttggaaat cgccaaaatc cacaaaggcg gcttccgcat
    33121 caccgccaac caaaacctca tcgtggcaaa tgtgcccgaa ggaggcaaag cccggatcga
    33181 acagctcgcc cgctcatacg ggctgatacg cgacggtgtc agcaagctgc gcgaaaatgc
    33241 gatgtcgtgc gcttccttcc cgacctgccc gctggcaatg gcggaagccg aacgcgtgtt
    33301 gccggacttc atcggcgagc tggataagat tatggcggaa cacggcgcgt cggacgacta
    33361 catcgttacc cgcattaccg gctgcccgaa cggctgcgga cgggcgatgt tggcggaaat
    33421 cggactggtc ggcaaagccg tgggacgcta caacctccac atcggcggcg accgcgaagg
    33481 cgtacgcatc ccccgcctct acaaagaaaa catcaccctg ccggaaatcc tttccgaatt
    33541 ggaagacctg atcggcaaat gggcggcggg acgcgatacc gacgaaggtt tcggcgattt
    33601 cgccatacgg acgggcatcg tcaaacccgt gttggatgcg cccgtcgatt tttgggacgc
    33661 atccaaagcc gtcccgattg cgcgcgcctg aacccgtccc ccgcattcct gcggggatgt
    33721 tcttttttgc cgcacacgtt tccattccgc aaaattccga tacttggtat aaaatccccc
    33781 cttttcccac tctaaaaaac cgtacccgat gccgtctgaa acagccctgc ctttcagacg
    33841 gtataggcac aaggaaacac actatgccag aataccgctc caaaacctcc acccacggcc
    33901 gcaatatggc gggcgcgcgc gcattgtggc gcgccaccgg cgtgatggaa accgacttcg
    33961 gcaagcccat catcgccgtt gccaactcct tcacccaatt cgtgcccggc catgtccacc
    34021 tgcacaatat gggccagctg gttgcccgcg agattgaaaa agcgggtgca attgccaaag
    34081 aattcaacac catcgccatc gacgacggca tcgccatggg gcacagcggt atgttgtact
    34141 ccctgcccag ccgcgatttg attgccgact ccatcgaata tatggtcaac gcccactgcg
    34201 ccgacgcgct ggtgtgcatt tccaactgcg acaaaatcac cccgggaatg ctgattgccg
    34261 ccatgcgcct gaacatcccc accatcttcg tctccggcgg cccgatggaa gcgggcaagg
    34321 tcatcggcgt ggcaaacatc caacccgaac gccgtttgga cttgattgac gccatgatcg
    34381 aatcggcgga cgacaatgtc agcaaccggc aagtcgaaga agtcgaacaa aacgcctgcc
    34441 cgacctgcgg ctcgtgttcg ggtatgttta cggcaaactc gatgaactgc ctgaccgaag
    34501 cgctcggtct gtccctgccc ggcaacggtt cgtatttggc gacccacgca ggccgcaaag
    34561 aattgttcct cgaagcaggg cgtatgattg tcgaaatcac caaacgctat tacgagcaag
    34621 acgacgaaac cgtgctgccg cgcagcattg ccaccaaaaa agcgtttgaa aacgccatga
    34681 cgatggatat tgcgatggga ggcagcacca ataccatcct gcacttgctc gccgtcgcca
    34741 acgaagcggg cgtggatttc aaaatggcag acatcgaccg cttaagccgc gtcgtgccct
    34801 gcatctgcaa aaccgcgccc aacaaccacg actactatat ggaagacgtg caccgcgccg
    34861 gcggcatctt cgccattctg aaagaacttg acaaagcggg caaactgcac accgacgtgt
    34921 acacgatcca cgcgccgaca ctgaaagacg cgattgaaaa atgggacgtc accaatcccg
    34981 aaaacacccg cgccatcgaa cgtttcaaag cggctcccgg cggcgtgcgc accactcagg
    35041 cgttctcgca aaaccgtatt tggaaaaccc tcgaccttga ccgcgaaaaa ggctgtatcc
    35101 gcgacgtggc acacgcctac tcgcaagacg gcggtttggc ggtcttattc ggcaacatcg
    35161 ccgagcgcgg ctgcgtggta aaaaccgcag gcgtggacga gagcatcctc aaattcaccg
    35221 gccgcgcgcg cgtgtttgaa agccaggaag ccgccgtcga agatattttg ggcaaccaaa
    35281 tcgtcgccgg caacatcgtc atcatccgct acgaaggccc gaaaggcggc ccgggtatgc
    35341 aggaaatgct atacccgacc tcctacctga aatccaaagg cttgggcaaa gcctgcgccc
    35401 tcttaaccga cggacgcttc tccggcggca catcaggttt gtccatcgga cacgcctcgc
    35461 ccgaagcggc ggaaggcggc gcaatcggtt tggtacacga aggcgatacg attgaaatcg
    35521 acatccccaa acgcagcatc cgccttgtca tttccgatga agagcttgcc gcacgccgtg
    35581 ccgaaatgga agctcgcggc agcaaagcgt ggaaaccgga aaaccgcgac cgctacgtct
    35641 ccgccgcatt aagggcttac ggcgcgatgg cgacttccgc cgacaaaggc gcggtgcgcg
    35701 acgtatcgca aatcgagaga taagccccaa aacgtgcggc aaatgccgtc tgaacagtaa
    35761 aggtatgatt attaacaaat ttatctgggc gatggcggta ttttccgcaa ttttggccct
    35821 cgttatcggc agcggcttta ccgcgctcga gtatgtaaaa gagccgcctg ccgcccctta
    35881 tgctgtttcc gcgccgaaaa ccgccggggt caagcctcgg cgcatcctgc cggaacggaa
    35941 tcccccgtgc tgccgattga cggttcaaac cccgcgcccg tttcaaacac cggcgatgcg
    36001 gacggacagg atacgcctga cggaaaggca gcagataccg tttccatcat cggggtcggc
    36061 ggcataaggt acgctgtttg acgaaggcgg tacgccgaaa aaatgtgcaa accaccaaaa
    36121 tatgctatgc attccgaacg ccctccgcat acgggcaata ccttgccgac gcgggattcg
    36181 actacctcag cctcgcaaac aaccacagca acggcttcgg cgcgcaaggc atcacggcaa
    36241 cggcaggcaa ccttgacgaa ttgaacatca aatattcggg catcgaaaac aggtttgaaa
    36301 ccgccatcct gaagaaaaac ggcgtgagat acggcttcgt ctccttcgcc cccaaccttg
    36361 ccgccgtcaa actgaacgat tacgccaaat tcaaaaaact gattcggaaa accaaacaaa
    36421 aaaccgacat cgtcattgtg atgttccacg gcggcgcgga agggaaacag gctgaacacc
    36481 tgccgttcga taccgaaatc ttctatgggg aaaacagggg caacgtcgtc gagtttgcgc
    36541 ggctcgccgt cgattccggc gcggatgtcg tattcgggca gggggcgcac gttactcgcg
    36601 ccgtcgaact ttaccacgac cgcttcatct cttacagcgg cggcaacttt gccacctacg
    36661 gcatcagcgg cattgcgccg attttcaaaa ttatcaccga caaacagggg cggttcgttt
    36721 ccggcaacat tatccccatc actcaagtcg gcgataaaat ccccaaaatc gaccctgaaa
    36781 aaactgttat tgagcggatt atttatctga accatagcga cttccccaac gggaacggac
    36841 tggatgtctc gcccggcggc gacatcacgc gccggtaaaa agatattgga aattccgtcc
    36901 gaacaaatgc cgtctgaaag ccttttttca ggtttcagac ggcattcaag tttcagacgg
    36961 catttttcaa tcgtcaataa tccgctttaa tcccggaggc tttttccact tcggcgacct
    37021 cgccttccgg ctcaaactgc aagggataca ggttaagctt ttccatcagg attctatcgc
    37081 cgtcctcatc aggattgcct gtggtcaaca gcttgtcgcc gtaaaaaatc gagttcgcac
    37141 ccgccatgaa gcacatcgcc tgcatcgctt caggcatatt gctgcgccct gccgagaggc
    37201 ggacataact ttgcggcatc gtaatccgcg ctacggaaac cgtacggaca aattcggtcc
    37261 aatccaaatc ttcggcatcg gcaagcggcg tgccttccac tttgaccaac cggttaatcg
    37321 gcacgctttc gggctgcggg tcgaggttgg cgaggctggc aatcagcccg gcgcgttcgg
    37381 cgcgggtttc gttcatcccg acgatgccgc cgcagcagac tttcaaaccg gcgttgcgga
    37441 ctttgcccaa ggtatccata cggtcttcgt gccggcgtgt gtggatgatg tcgttgtagc
    37501 ggtcggggtc ggtgtcgagg ttgtggttgt aataatccaa gcccgcctct ttcaagtctt
    37561 ccgccatacc ttcttcgagc ataccgaacg tgccgcaggt ttccatacct aagcccttga
    37621 cggctttgat gattgcggaa accgtctcca cgtctttggg tttggggccg cgccatgccg
    37681 cgcccataca aaaccggctt gcgccgcgcg atttggcgat tttggctttt tcgacgattt
    37741 catccacatc catcatctgc tctttgccca aattggtgtt gtggtgcgct gattgcggac
    37801 agtaggcgca atcttcggga caaccgccgg ttttgatgga caacagcgtg gaaagctgga
    37861 tttcgcgcgg gttgaaattt tggcggtgga cttctgccgc ctgatagaca agttctaaaa
    37921 acggcagtcc gaaaagtgcc tcgacatcgc attttttcca atagcgcgcg gtgggatgag
    37981 gcttgcactc ggtcttacgg cgcaaggcga cgggggatac ggtcataatg tgttctttcg
    38041 tatttacagc ggcgcaatga cgctgacagg caaaccgccg gcgcaatgac ggcggttgcg
    38101 ggtaatcaaa tggctgaaaa cagcggcggc aagtgtaacg cgtttgaaaa tacggggcaa
    38161 acggtttttc cgcctttgtg ccgcgcagcc gggcaatgcc gtctaaaggg ctttcagacg
    38221 gcattgcccg ggctatctca aagcgtccag cagtttggtg tgtattccgc cgaaaccgcc
    38281 gttgctcatc accaaaatat ggtcgccggt tcgggcgttt ttcacaattt cggcaacgaa
    38341 ggtatcgaaa tctttaccga cgcgcagcct gcagcccaaa ggcgcgaggg cttcggcaac
    38401 gtcccagtcc gcgccgccgg cgtagcagaa cacttggtcg gcttctttga ggcttgcggg
    38461 cagggcggac ttcatcgtgc cgagtttcat ggtgttggaa cgcggctcga ggacggcgag
    38521 gatgcgcgcg ccgccgacac gttggcgcaa accttgaatc gtggtttcga tggcggtcgg
    38581 gtggtgggcg aaatcgtcgt aaacggtgat gccgtttgcc gtgcctttga tttccatgcg
    38641 gcgtttgacg tttttaaacg cacccaaggc ttcgcaggcc gtctgaacat cgactccggc
    38701 atggcgtgcg gcagcgatga cggcgagcgc gttcatgcgg ttgtgtccgc ccatcaaatc
    38761 ccatgcgacg tgtccggctt ttttgccgtc aagcaatacg tcgaacgagc cgtcggcatt
    38821 gacttcacca atctgccagc cgtgtccggt gccgaatttt tccaccggcg tccagcagcc
    38881 tttgtccaaa gtatcttgca ggctttgctg ctgtccgttg cagacgatga ggccttcgga
    38941 tggtacggtg cgcacgaggt ggtggaactg ggtctgtatc gcgcccaagt cggcgaagat
    39001 gtcggcgtgg tcgaattcca gattgttcaa cacggcggta cgcgggcgat aatgcacgaa
    39061 tttggagcgt ttgtcgaaaa aggcggtgtc gtattcgtcg gcttcgatga cgaaaaacgg
    39121 cgatttgctg ttcgggtctt gacgcggcgt ttgcggtagg cgggcggaaa cgccgaaatt
    39181 ttccggtaca ccgccgatga ggaagcccgg cgcgagtccg gcatattcca agacccaggc
    39241 gagcatggac gcggtggtcg ttttgccgtg cgtccctgcc acgccgagta cccaatgatg
    39301 gtgcagcacg ttttcagcca gccattgcgg gccggaaata taaggcagcc cacggttcaa
    39361 aatcgcctcg accacatcca tcccgcgcct ggcgacattg ccgatgacgt aaatatcggc
    39421 ttgaaattcc tccaactgcg cggcatcgaa gccctcgtgt acgcctatgc ccaaggcttc
    39481 gagctgggtg ctcatcggcg gatacatctt cgcgtcgcaa ccgctgactt tgaacccggc
    39541 ttctttggca atggcggcaa tcccgcccat aaacgtgccg ccgataccga taatgtggat
    39601 gtgtttcatc agagttccct aatcgttcaa attctattgt tcaacaccgt aatggttgat
    39661 tcggtttcaa tcaatgcggt ctgatgccgg catccgaacc gtctttctgt gcattccctt
    39721 tcggattttg gacggcttcg tccctcaccg tgtccgaata atcgccaact cctgcgtctt
    39781 ccgccctgtc tttcacggca ggcctgaccg aactgtacac ccgatcccgc cccaaacccg
    39841 cgttggtaca atgttttccc ggagtttggc tgtaactggt tttaccatta tggcggcact
    39901 cgtaaacatt tgccgaaaat acggtctgaa ccgctgcaac aagcaatata cttccaatca
    39961 ttttagatac gcgcatatta aactccttta ttgtttctct tcaaaaaaag gaatgtattt
    40021 caatagaact tcatctaatt ctttcattcc ttgctcataa ttgaacgggt attcccaacc
    40081 atcccctact ttgatttcag aaatgtcttt aaacttccgc ttaagggcgg acactttgtc
    40141 catatcgtca tcaatttcct gtttgccgaa cggtattttc cgcttttcca aaacctcgtc
    40201 ataataacaa ggtaaattca gcaatagccc ccaagcgcta tttgcgatta cgactaatct
    40261 atatttttcc ctagttaagt aatctgcacc gctatcatag attgcccttg tcggatcata
    40321 aaaaaattcg caatttttca tagcgtcccc acactttaac cgattcgcaa ttttatatcc
    40381 gcgattttgc aagctgccca taaaaaccga ttgcctgcca agccgacact acttgttaaa
    40441 gatgcccgat aatgccgcaa ctcttccgta tttaccgaca aacgccctat ttgcttagag
    40501 ctgttttgtt ttccacaaaa ctttaatccg ttgatttaac aaatttttta ccaaattctt
    40561 tatttgttcg gattccatta gcacgatttt gagcaatatc tgtcaaaacc atttccacca
    40621 tatccaaaac aaaaccgccg ccgccacggc attggacgat aagatgagtt tccactgccc
    40681 gtccttccgc aatgccgcct tcttgggctt caaagcaccc gcataaaaaa acggtgacag
    40741 cagcatagaa gcgatacata agaaaatcaa tacgccgtaa tagatttttt cagacggcat
    40801 ttctcttcct caatacgccg cccgttggcg catctgttca aacaggcaga ttgtcgccgc
    40861 catcgcgaca tttaaagact cggttgcatc gtgcatcggt atcctgacac acttgtccgc
    40921 cctgtctaaa actgctttac cgacacccgc accttcgttg ccgaacaccc aagctgtcgg
    40981 ttcgcacaaa tcttcgccgt acaaaaccgc ctgcttttcc tcgcgcaacg cggtggcaaa
    41041 cacacggtct tcatagtgcg ccaaccatat ttccaaatcc gcctgcgaat aaatgtccaa
    41101 caagaaatgc gcgcccatac ccgcgcgtaa taccttgggc gaccacgcgt ccgcacaacc
    41161 cctgcccaaa acgaccgtac ctacccctgc cgccgccgca ctccgtaaga cggtgccgac
    41221 attgcccgga tcttgcacgc cgtccaaaac cgcacagtcg cctttgtccg gcaaggttcc
    41281 gcccaatggg atatcaatca gtgtaagtat atcgtctgca caactcaagc tgctgatttt
    41341 tttcaatatg ccgtctgaaa cggaaaaaat cccgtcttcc ggcaaaaccg ctgtcaattt
    41401 aaggacttct tcagacggca ttttagcttc gggaatatat accccgaccg gctttctgcc
    41461 ggattgcagg aaaacctgaa gcaggtgcac gccctccaaa acggtttggg catactgccg
    41521 tctgaacttt ccttgcgaca acaggcggtg caggtgtctg atatgttcat tattggtcga
    41581 actgatgtgt ttcacttcac aagcctttta gaaatatgca accggacaat acccgactga
    41641 acgccgccga aatctgtttg cgatattatc aaaataaaat gggaaagtca ggatgtacgg
    41701 gcaaaatgcc gtctgaaacc gagatttgaa aatccgcaca acagtttgcc aacgccaatg
    41761 gacggtttcc gctgtcggca acgagcggcc aaattgggcg caggaaaggc agaacccgtt
    41821 tcccctgaag gatttttttc acatttttat ggatgccgcc gacagccaac gtatccgatg
    41881 ccgctaccgt cctcaaaatc ccgtcctgtt ccaacatggc ttcaggaagt ccgaacggat
    41941 gccgcttcaa cacaaatcta tgttctgtca gtattttttt gagatttccg cttatcgtcc
    42001 catctttcag aaaagccggt tttgccaact gttccgacgc aaacacaaac agcctgcctg
    42061 cataatgatg caattcaaag ccttgcaagt tccaacgtcc ggtttttgcc tccgtcaaaa
    42121 cgcgggcaat gtcggcaagt gcgttctgat tcggcacggg gatgccgttt tcctttagaa
    42181 aattccgcaa aatatgggtt ttccggcgcg gggaaaacgt cagccaccgc gccgtatcga
    42241 aataaccggc cccgcaaacc caacggcagt cttgaacgac gacctcttcc aacaaagcca
    42301 aatcttcctg caaagcgcgg acattgttca gcacatgccg cccgaaatgg ggaatctgcg
    42361 ccgaaagttc gggcaaaata cggtgccgga agcggtttcg caaataagcc gtatcggtat
    42421 tgctttcatc ctcgatattc ggcaaaccgt gttttcgggc ataatcccat atgtcttggc
    42481 gcgaaaaagg cagcaaaggc cgccagataa tgccgttttc ccccaaaggg cggacggcgg
    42541 gcatagccgc caaagcgcgc aaacctccgc cgcgcgcgac tgccagcata aaggtttcga
    42601 tttgatcgtc cctgtggtgc gccaacgcca aaacgtcaaa gcctttttcg gcaaactcgg
    42661 cataacgctt ttgccttgcc gccgcctcga tgcccaaacc gtttttttcc acgcagacct
    42721 taaccgtttc cagccccacc cctagcatat cgcaataatt ccggcagaaa tccgcccaat
    42781 cgtcggctcg gggactcaag ccgtgatgga tgtgcaatgc ctccggcaca aatcctgccc
    42841 gcttcccggc acaaaccagc agatgcagca aaacgacgga atccaagccg ccgcttaatg
    42901 ccactgctgt tttttttcca ttcagacctt gaggaaaaca atccttcaag cattgctcaa
    42961 acgcatctaa agtcagcacg gcttcatccc gataaagaaa aagcaagcag tttcagacaa
    43021 actgcttgct gcttgttcta cccgaattat tgttccgaaa atttgccgta agccataatg
    43081 cggtcgaaac ggcgcgaaag caaatcggca agcgggatgc tttgcgcttc gtgcagttgt
    43141 ttttccaaaa cggcttttac gtttttcatg gtttgcccga aatccctatg cgcaccaccc
    43201 aaaggctctt taatgacggt atcgaccaag tccagctttt gcaggcggtc ggcagtaata
    43261 cccaaagcct gtgccgcatc agccgccttt tcggcggttt tccacaaaat cgaagcacag
    43321 ccttcggggg aaataacgga ataggtcgaa tattgcagca tattgacgta atcgcctacg
    43381 gcgaccgcca acgcaccgcc cgaaccgcct tcgccgatga cggtacacaa aacgggaacg
    43441 cgcagtcggg tcagttcgta caggtttttg ccgatggctt ccgactgccc gcgttcttcc
    43501 gcgccgatgc cgggatacgc gcccggcgta tcgataaagg tcatgacggg caagccgaat
    43561 ttttccgccg ttttcatcag gcgcagggct ttgcggtagc cttcaggacg gggcataccg
    43621 aagttgcggc ggattttttc tttggtgtcg cgccctttct gatgcccgac gaccacaacg
    43681 ctttgtccgt tgaaacgcgc caatccgccg acaatcgcat gatcgtcggc aaagtggcgg
    43741 tcgccgtgca gctcttcaaa atcggtaaac agcgcgtcaa tgtaatccaa agtataggga
    43801 cgctgcggat gccgggaaac ctgtgaaatt tgagcgggtg tgagtttgct gaaaatcgat
    43861 ttggtcaaat cgttgctttt tttctgcaaa cggtgtattt cgtccgaaat atcgacggca
    43921 gactcgtctt ggacgaaacg cagctcatcg attttgttgg tcagttcggc tatgggttgt
    43981 tcaaaatcca aaaaaacagg tttcataaaa tgaagctctc atcaacagat tctgccgcta
    44041 tcatacgcta tttgtgcata ttccgcagtt aatttattgt ccaaaacggc ttttccacac
    44101 cctctttttg tgttcggaca gcatggaaaa cagcggatta gtgcatatat actagttata
    44161 aggcgatgta tcttatgcta aactcgaaac aaattcaatt atttcaaaca aaatcgtatg
    44221 aaagacaagc acgattcttc cgccatgcgg ctggacaaat ggctttgggc ggcacgtttt
    44281 ttcaagaccc gttcccttgc gcaaaagcat atcgaactgg gtagggttca agtaaacggc
    44341 tcgaaagtca aaaacagtaa aaccatagac atcggcgata ttatcgacct gacgctcaat
    44401 tcccttcccc ataaaatcaa ggttaaaggt ttgaaccacc aacgccgccc ggcacccgag
    44461 gcgcggcttc tgtatgaaga agacgcgaaa acggcagcat tgagggaaga gtgcaaacag
    44521 ctcgaccaat tcagccgcat cacttccgcc catcccgacg gcagaccgac caagcgcgac
    44581 cgccgccaac tggacaggct gaaaaaagga gactggtaaa accattcccg ctgcaaacag
    44641 aagcacgaac cgcgtaccgc cgcatttcag acggcattcc tgaaacttat tccgcctcgt
    44701 ctatccatgc ctgctgcacg gcttcaagga tgcgttcgcc gcaacgggcg ggatcgtcgt
    44761 caaattcggg caatgccata atcaggtcgc gcagttgggt aaagcgcacg gttttgggat
    44821 cgatggcttc gccgtgcagg tcgtagagtt cttcggcgat gcgccgggta tcggtccatt
    44881 tcatataaat ttccttttat cgtccggcat tgaagccgtg tagaatatgt ttcggcaaaa
    44941 ctgccgcccc ttgaggatgg ctgaatgttt caactcgccg gacggcacat caagcaccac
    45001 cttatgtgtc gtcctgatcc gggaggagtt acccctccca aacaaatctg attctaccgc
    45061 cctgaagagc ggggtttcaa ccgataagga agattgatga acaatatgtt tgccgcaaaa
    45121 ttgtccgaac tggtttatac cgcttccgac catcccgact ccctgtcgga gatggaggag
    45181 tttgaccgcc tgattctgct gatacgcaaa ctgtatcaaa tattggacgg gcaacatacc
    45241 ctctacagag taacggtttg ccttaactac cggagcaggc gcggtctgat tgctttggat
    45301 aaagcggctg tcggttgcga tgcggcaatg ttgcgcgccc ggcgttggcg catctgcatg
    45361 cagattgcgg aacgcctttc cgagtcggtc gggcggcttc acggttacgg cagaaagcgt
    45421 gtccgccgcc tgccccgagc ttgaaggacg ctatctcgac cttgtccgcc gcgccgccgt
    45481 ctctttcggt ttcgcgcaag gacaggcaaa tccggcaggc gggcaggaag aaacgcaagc
    45541 cgcctcagat gccgtatcgg gtcgttccgt caaaaaaccg ataaaaatca atatagaatc
    45601 gaaagttccg tcttcacaaa cgtctgtttt tcggtaattg cgccttatcc gcgcaacaat
    45661 gccgtctgaa gcgtgccggc ttcagacggc ctgttttcag tgttcttcgc gcgcgtggtt
    45721 gatggtgtat ttagggattt ccacaatcaa atcctcgccg gcaacaaccg cctgacaact
    45781 caggcgcgaa tcggcttcca aaccccaagc ctgatcgagc aggtcttctt ccaattcggc
    45841 cggctcttcc aaactgtcga aacctttgcg gataatcacg tggcaggtgg tgcaggcgca
    45901 ggatttttcg caggcgtgat cgacttcgat gtcgtggtcg agcagcacgt cgaggacggt
    45961 tttgccttcg ggcgcgttat cgatgactgc accctcgggg cataatgttg cgtgtggaag
    46021 tacggtgatt ttgggcattt ttattgtctt tctgtaaaaa gtatttgtgt ccgtttttag
    46081 gaaaactgtc tgcctaatgt cgcggttcga gcaggtcggc aacattttgc cagcccgtca
    46141 ggcgggcaaa atcgagggcg gtatagcctt ccgatgtctt tgaggcagga tttgcgccgt
    46201 attcaagcat ggcttgaacc ataagtgcat ttcctgccat ggcttcatga tgaagcggcg
    46261 tatagccgtc ttcgtcctgc tcatgcagaa aatccggatt ttgggacaac tgttgccgaa
    46321 aatcgtcaat acagttccgg cacatcggat gctcttccaa attttcggga tgttcttttt
    46381 cttcatacac cggcaatacc tgcccgggat cgccgaaatc gattccccag gcggtatcgt
    46441 gttcggtacg ctcctcttcc gtcatctgtc cgcgcattgc ctgtacggta aagccgccgt
    46501 aggggatgcc gttgcacacg aacatccagt cgctgatgtc gccaaccgga acgcaaacgc
    46561 tttcgccttg ttcgacattg gtcagtccgc cgggttcatt gttcagcaca ccgtaaatat
    46621 aaagaccgtc aaaataaata tcgtcgatcc acatatgttc gcaaatttcg ccgtcttcgc
    46681 cgtcttggaa aaaagggact ttgaccatgg caaaatccaa ggcggaaata atgcggcggc
    46741 gttcccaaaa aagctcgcgc caaaagtatt tgaatgtttt acgggcgcgt tcgccggcac
    46801 ggtttaccgg ttcgtctgcc tgttctacat aataaatgac ggaatcgccc atcatactgc
    46861 tcctcaacgt gtacggtatc tgtttgcacc ttaccgcggt tttctacctc cggcatccga
    46921 ttcggatttg aaaagttcca aatattcgga ataggctttt tccaaatccg cccgcgaaaa
    46981 acaggtgtaa atatggatgc cgtctgcaat ttcgtcggtc agcctcgtgc cgcttttgcc
    47041 gaaatatgct tctttaaaaa ggcggatttt cgattttttc ggcttgtagg atgccgcgtc
    47101 cgccaaaagc cggggcagcg cgtaagggta ttcccggtgt ccgcgccgta ccaaatcaag
    47161 cagttgtttg cggccggacc cggcattccc ttcctgccgg cgttcgtgga cgcttttttc
    47221 aaattccgat tccgccgttt cgtttttcgg gaacacgatt tcgccgcgaa gcgataaaaa
    47281 caaacgtttc acaaggtttt cgtccacggc gaacaattcg gtatcgccta tcctgctttt
    47341 gctgaaaatt tcatggagca ggtttttttt ctttgtaatt gtcggttttg acggcgagga
    47401 ggcgttccaa cccggcaacg ttcgcatagc cgttgttttc caaatggcgc attctgcctt
    47461 caaaattgct tacgtccgaa atgccgattt tatatagtga attaacaaaa atcaggacaa
    47521 ggcggcgagc cgcagacagt acaaatagta cggcaaggcg agccaacgcc gtaccggttt
    47581 aaatttaatt cactatacac gcccttgatg acggttttca tcgaatagac aatgcctgac
    47641 ttttccatat cgatgttttt caagtgtttt cgatccttca gacggcatca ggttatatct
    47701 atgccgtctg aaaccgttta agtatcaaat attatcgaca ctctgacctg tcagcgcacg
    47761 ttggatgttg cggttcatgc gcttggcggc gaaattgtcg gtgctgcggc tgagtttggc
    47821 gacagctgca cggatgtctt cagctttacc gtctttcaga cggccttgca aatcggcgat
    47881 gtctcgctgg atttgtgcaa actcttcggc atccagcaaa tcgctgtcca actcgagggc
    47941 ggcgttgacg gcatcggtca ggctttcggc ttcgaccacg gcttcggcgc gggcgcgtgc
    48001 cgccatatct tcggcggcgt tgcccatgct gtctttgagc atttgggtga tggtgtcatc
    48061 gtccaaaccg taggagggtt tgacttcgat ttgcgcctgt acgccggtgc tttgttcttg
    48121 ggcggaaacg gacagcaaac cgtcggcatc gatttggaag gtaacgcgga tacgcgccgc
    48181 acccgccgcc ataggcggaa tgccgcgcag ggtaaatttg gcaaggctgc ggcagtcgga
    48241 aacgagctcg cgttcgcctt gcacgacgtg tatcgtcatc gcggtctgac cgtctttgaa
    48301 ggtggtaaag tcctgcgcgc gcgcggtggg gatggtggaa ttgcgcggga tgattttttc
    48361 cgccaagccg ccgtaggttt ccaaaccgag cgacaagggg gtaacgtcca acagcagcca
    48421 ttcgccgtcg gctttgttgc ctgcgaggac gtttgcctgt atggctgcgc cgagcgcgac
    48481 gacttcgtcg ggattgaggt tgttcagcgg ggtttgtccg aaaaaggtgg cgactgcctg
    48541 ttggacgtgc ggcatacggg tcgaaccgcc gaccataacc acgcctttga cttcgttttt
    48601 accgacaccg gcatctttca acgcctgttt gaccggctcg aacgttttca tcaccaaatg
    48661 ctgcgtcagg ttgtggaact cggcgcgact gatgcttgtg tcgattgcca taccgtctga
    48721 aaacgtcgcc tgaatgcgcg cttcggtttg cgtggtcaat tgttctttgg cggcgcggac
    48781 gagcgagagc aggagttggc tgtcttgttc gttgagttgg gagagtctgt tttgttcgag
    48841 caggtagcag aacaaacgat ggtcgaagtc atcgccgccc aacgcgctgt tgccgccggt
    48901 ggctttgact tcaaacagtc ctttggtcag ttgcaacacg gatacgtcga atgtgccgcc
    48961 gcccaaatcg tacacgacaa acgtgccttc cgaggcgttg tccagcccgt aggcgattgc
    49021 ggcggcggta ggttcgttga gcaggcgcaa tacgttcaaa ccggccagac gcgcggcatc
    49081 tttggtggcc tggcgttgcg cgtcgtcgaa ataggcgggg acggtaatca ccacgccgac
    49141 caaatcgccg cccaaggttt cttcggcacg caatttgagg gttttgagga tttccgccga
    49201 cacttcgaca ggcgttttca ccccctgccg cgtatgcagt tcgataaagc gttgattgtc
    49261 gccgaaacgg taaggcaggt aatgcgtatt ttgatgcaga tcggcaagag tccgcccgat
    49321 aaggcgtttg gcggagctga cggtgttcag cgggtcggtt ttttgggcgg acagggcggt
    49381 tttgccgact tcaatgccgc cgttttccag atagcggacg acggaaggca gggtaacgcg
    49441 cccgtcggca tcgggcaggc aggcggcact gccgctgcgg acggtggcga ccaagctgtt
    49501 ggtcgtaccc aaatcgatgc ctgccgccaa acggtgccgg tgcggggcgg cggacatacc
    49561 cggttctgaa atctgcaaaa gagccataaa ttttgcctgt cggtgcggtg tgtaaaggaa
    49621 ggaaggcggc attttaacag aatttgcgcc gatagttgag caaaccgcta aggattgggc
    49681 cgtgccgccg aaattgccct ctaccgccaa ataccgataa aatccgcctt tcccgcccat
    49741 cattaggaat ataaatcgtg aaaggactcg actattgccg ccaaaaagca gaggaaagcc
    49801 gctccagttt tttgtcgggc ttccgtttcc tgactcagga aaaacaggat gcggtaacgg
    49861 ttctgtatgt cttttgccgc gaattggacg atgtggttga cgaatgttcc aaccccgatg
    49921 ttgcacaggc aacattgaac tggtggcgcg gcgatttgga caatgcgttc ggcggcgcga
    49981 tgccggaaca ccccgtcaat caggccttgc ggcaggttaa ggaaaccttc aagctgccga
    50041 aatatgaact ggaagcctta atcgacggga tgcagatgga tttggttcag gcgcgttacg
    50101 gcagttttga ggaattgaaa ctgtattgcc gccgcgtcgc gggcgtggtc ggctgcctga
    50161 ttgcgcggat tttggggttt tcagacggca aaacgctgga atacgcggat aaaatgggtc
    50221 ttgcgctgca actgaccaac atcatccgcg atgtcggcga agatgcgcgc aggggacgga
    50281 tttacctgcc gatggaggaa atgcagcggt ttgacgtacc cgcaagcgtg attttgcaat
    50341 gcagcccgac gggcaatttt gccgaattga tggcgttcca aatcaaacgc gcccgtgaaa
    50401 cctaccgcga agccgtatcg ctgctgcctg atgccgataa aaaagcccaa aaagtcggac
    50461 tggttatggc ggcggtttat tacgcgctgt tgaacgaaat cgaccgagac ggcgcgcaaa
    50521 acgtcctcaa atacaaaatc gccctccctt cgccgcgcaa aaaacgcatt gccctgaaaa
    50581 cctggttatt cggattcaaa ccgcgccccg gcacgccgga acgggcataa ggcgcatact
    50641 gcccttcagg aaaacccaac ccccgccgcc ccgaaaaacg gggtttcaac cattggagga
    50701 ataaatgatg aacacgccgc atccgcgccc gaaaatcgcc gtcatcggcg caggctgggc
    50761 cggcttgtcc gccgccgtca ccttggcgcg gcacgccgac gttaccctgt ttgaagccgg
    50821 ccggcaggcg ggcggaaggg cgcgcacact ggccggaaat accgacggtt tcggtttttt
    50881 ggacaacggg cagcacattt tgctcggcgc ataccggggc gtgttgcgcc tgatgaaaac
    50941 catcggttca gacccccgtg ccgccttttt gcgcgtaccg ctgcactggc atatgcacgg
    51001 cggtttgcag ttccgcgccc tccccctgcc cgcgccgctg catattttgg gcggcgtgct
    51061 gcttgcccgg cgcgtaccgt ccgcattcaa agccaaactg cttgccgata tgtccgattt
    51121 gcagaagtcc gcacgcctcg gacagcccga cacgacagtt gcacaatggc tgaaacagcg
    51181 gaacgtgccg cgtgccgccg taatgcagtt ttggcagccc ttggtctggg gcgcgctcaa
    51241 cacgcctttg gaaaccgcaa gcctgcgcgt gttgtgcaac gttttgtccg acggcgtgct
    51301 gacgaaaaaa tccggcagcg actatctcct acccaaacag gatttgggcg caatcgtcgc
    51361 cgaacccgcc ttggcggagc ttcaacggct cggcgcggac atccgcctcg aaacgcgcgt
    51421 atgccgtctg aacaccctcc cggacggaaa agtcctcgtc aacggcgaag ccttcgatgc
    51481 cgccatactt gccaccgcgc cctaccacgc cgccgcgctc ctgcccgaag gcacgcccga
    51541 acacgttcag acggcatatc aaaaccttcg ctaccacgcc attaccaccg tctatctgcg
    51601 ctacgccgaa cccgtccgcc tgcccgcccc gctgaccggc attgccgacg gcacggcaca
    51661 atggctgctt tgccggggca ggctcggact gcccgaaaac gaagtctccg ccgtcattag
    51721 cgtttccgac cgcgtcggcg cgtttgcaaa ccgggcgtgg gcggacaaag cccacgccga
    51781 cctcagacgc atccttccgc atttgggcga acccgaagcc gtgcgcgtca tcaccgaaaa
    51841 acgcgccaca accgcaaccg atgccccgcc gccggatttg tcgtggctgc accggcaccg
    51901 tatcttcccc gccggcgact acctccaccc gcactacccc gccacgctcg aagccgccgt
    51961 acaatcaggt ttcgcgtcgg cggaagcctg cctgcaaagc ctgaacgatg ccgtctgaaa
    52021 acgccggccg gcatcgggac gctttacaag gtgcggcaaa acgctaaaat acattaaccg
    52081 tcaaacccaa aggacaacca tgccgacact gaccgacaaa accatcttag ttaccggcgc
    52141 atcgcaaggc ttgggcgaac aggtcgccaa agcctacgcc gccgaagggg cgaccgtaat
    52201 tctggtggca cgccatcaga aaaaactgga aaaagcctat gacgcgattg tcgaagccgg
    52261 acaccccgaa cccttcgcca tccgtttcga cctgatgagt gcggaagaaa aagaattcga
    52321 acggtttgcc gccaccattg ccgaagccac gcaaggcaaa ctggacggca tcgtccactg
    52381 cgccggctat ttttacgccc tctcgccgct ggatttccaa accgtcgccg aatgggtcaa
    52441 ccaataccgc atcaacaccg tcgccccgat ggggctgacc cgcgccctgt tcccgctgct
    52501 gaagcagtcg cccgacgcgt ccgtcatctt cgtcggcgaa agccacggcg aaacacccaa
    52561 agcctactgg ggcggcttcg gcgcgtccaa agccgcgttg aactacctgt gcaaagtcgc
    52621 cgccgacgaa tgggaacgct tcggcaacct gcgcgccaac gtcctcgtcc ccggccccat
    52681 caattccccg caacgcatca aatcccatcc gggcgaagcc agaagcgaac gcaaaagcta
    52741 cggggacgta ctgcccgcat ttgtctggtg ggcaagtgcc gaaagcaaag ggcggagcgg
    52801 cgaaatcgtt tacctctaaa tccggaaaag gcaggcatcc cgcccgcgcc gcgcttccgt
    52861 tttgaaaaat atccgtacct tccgcgcgca tccgtcccgc acagacaaac accttgactt
    52921 aaatttacat aaggacaata atgataaccc gttatttccc taactggacc ttagggtaaa
    52981 ttttatgaaa accgtttccg ccgccatcgc ttttgccgcc gctgccgttt cactgaccgg
    53041 ctgtgcgacc gagtcctcac gcagcctcga ggttgcaaaa gtcgcctcct gcaatacgca
    53101 atatcacggt gttcgcaccc cgatttccgt cggaacattc gacaaccgct ccagcttcca
    53161 aaaaggcatt ttctccgaca gtgaagaccg tctgggcagc caggcaaaaa ccatcctggt
    53221 aacacacctg caacaaacca accgcttcaa cgtactgaac cgcaccaacc ttagcgcatt
    53281 gaaacaggaa tccggcattt ccggcaaagc gcagaacctg aaaggcgcag attatatcgt
    53341 taccggcgat gtaaccgaat tcggacgcag agatgtcggc gatcatcagc tcttcggcat
    53401 tttgggtcgc ggcaaatcgc aaatcgccta tgcaaaagtg gctctgaata tcgtcaacgt
    53461 caatacttcc gaaatcgtct attccacaca gggcgcgggc gaatacgcac tttccaaccg
    53521 cgaaatcatc ggtttcggcg gcacttccgg ctacgatgcg actttgaacg gcaaagtttt
    53581 agacttggca atccgcgaag ccgtcgacaa cttggttcag gctgtcgaca acggcgcatg
    53641 gcaatccaac cgttaaggag aaatgatgaa tccgaaaacc ttgggccgtt tgtcgctgtg
    53701 tgcggcggtc ttggctctga ccgcctgcgg cggcgggcat aaaaacctgt attattacgg
    53761 cggttatccc gataccgtct atgaaggttt gaaaaacgac gacacttcgt tgggcaagca
    53821 gaccgaaaag atggaaaaat actttgcgga agccgccaac aaaaaaaatg aatgccgccc
    53881 cgggtgcgca cgcccatttg ggactgctgc tttcccgttc gggagacaaa gagggcgcgt
    53941 tccgccaatt tgaagaagag aaaaggctgt ttcccgaatc gggcgtattt atggacttcc
    54001 tgatgaaaac cggtaaagga ggcaagcgat gaaacctttg attttagggc ttgccgccgt
    54061 gttggctctg tctgcctgcc aagttcgaaa agctcccgac ctcgactaca cgtcattcaa
    54121 agaaagcaaa ccggcttcaa ttttggtggt tccgccgctg aacgagtcgc ctgatgtcaa
    54181 cggcacttgg gggatgctgg cttcgaccgc cgcgccgatt tccgaagccg gctattacgt
    54241 cttccccgcc gcagtcgtgg aggaaacctt caaagaaaac ggcttgacca atgccgccga
    54301 tattcacgcc gtccggccgg aaaaactgca tcaaattttc ggcaatgatg cggttttgta
    54361 cattacggtt accgaatacg gcacttcata tcaaatttta gacagcgtga cgaccgtatc
    54421 cgccaaagca cggctggtcg attcccgcaa cgggaaagag ttgtggtcgg gttcggccag
    54481 catccgcgaa ggcagcaaca acagcaacag cggcctgttg ggggctttgg tcggcgcagt
    54541 ggtcaatcag attgccaaca gcctgaccga ccgcggttat caggtttcca aaaccgccgc
    54601 atacaaccta ctgtcgccct attcccgcaa cggtatcttg aaaggtccga gattcgtcga
    54661 agagcagccc aaataagcat atgccgtctg aacctctccg agcttcagac ggcatatgcg
    54721 aatcttgata cttgcaagcc gcaccgccca aaatcaggcg accggtttca atatccgaac
    54781 aaggttcggg ttctgtattt tgcagaaccc gaacctttta ttcaaattta aacttaattc
    54841 cggcgtggtt gtatggtaaa ttatagtgga ttaaatttaa accagtacgg cgttgcctcg
    54901 ccttgccgta ctatctgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc
    54961 cactataaca agcaatatgc cctgaccggc aaaacttccc caaacttcat caaaaaatca
    55021 actgtacccg tctttttcag acggcattga taccgtaaga gatttcccat gcaaaaccgg
    55081 caaaccgagc tttattccac gccgtcttgg ctgttgcaga ccttattgat gattaccgcc
    55141 gcctcggcgg tgattctgtt tttcgcgcgc aacacgcgct tggggcggga gtttgcctat
    55201 atcctgcgcc tttgcctgac accgaaaagc acggtcaaag tattgctgct gattacggca
    55261 atggttacgc tgctgttaac cgaagtgcgg ttgaatgtgt tgagtacctt catgtccaaa
    55321 ggactttacg attcgatgca ggatttgaat gcttcggcat tttggatgtt tgcagcgatg
    55381 aacgccggcg tggtactgat acgggcgttt aacaacgtcg ttaacgactt tctcgattaa
    55441 ggcttggcga ttaaatggtc ggaacggctc aatgaagttt tgacttcgcg ctggcttgcc
    55501 gacaaaaact actaccgcct gcaaatgcgc cgccacgcgc cggacaacat cgaccagcgt
    55561 atccaacagg atgcgcagga attcatcgct tcgaccatcg aatttgtgcg cggcatggtc
    55621 aattcggtcg ttacttctct ggaatttgcc gttgttttgt ggggcttggc aggcatcctg
    55681 accgtgttcg gcttcgacat tccgcacggc attgtttggt ttgtctttat tttcatgatt
    55741 ttggcgatct ttatcgccat gtggataggc aaccctttga ttcgttacaa ttatgaaaac
    55801 aaaaaactca acggcgacta ccgttattcc ctcatcctcg tacgcgacca cgccgaaagc
    55861 gtggcgtttt acagcggcga acaacacgaa cacggccggc tttccgaccg ctttaaagcc
    55921 attatccgca accgttggcg catcgcgcgg caaagcgtct gcttaagcgg ctttaacgat
    55981 atgttcacca acggcatcaa actcttcctg attattttgc aagctccgcg cctgtttgcc
    56041 ggacaaatca aaatcggcga catccagcag accgtccaag ccttcgcgcg actgcaaaac
    56101 gcgctgtctt tcttccgaat gttctacaac aaattcaccg cctgccacgc ccgactggag
    56161 cgtctgtacg tctttttgct gagtaccgaa gaacaacacg gcgcgcagca acccgagatt
    56221 agcgaagttt cagacggcat tgcactggaa aacgtcgccc tgttccgcca caacggcgaa
    56281 gttttattgg acggcatcaa catcaaactc aaaagcggcg attccctgct gatacgcggc
    56341 ccgagcggtt taggcaaaac ctcgctgctg cgcgcgctgg cggggctttg gccgttcggc
    56401 agcagcggca aagtcagccg tccgccgcat caaggcatcc tcttcctgcc gcaacgcccg
    56461 tacacggcac aaggcagcct gcgcgacgcg gtttgttacc ccgatatcga caaacagcat
    56521 cccgagttag ccgaagccat gaacacctgc cgcttgggtt atctgattga caaattggat
    56581 aaaacagacg gctggcagca caaactctcc cccggcgaac tgcaacgcgt cgccttcgtc
    56641 cgcgccctgc tctccaagcc caaaatcgtc ctgctcgacg aagccgccgc cgccttggac
    56701 gaaccggccg aagcctagct ctaccgcgcc ttaaagcaaa aactgcccga cagcatcatc
    56761 agtatcggac accgaagtac gctcaacgcg ttccgcaata tgcacctcga tgtcggcaac
    56821 gctgcctgcg gctgaacgat gccgtctgaa gccgttttca gacggcatcg ttcaaattgg
    56881 aaagacacgc gaaaacaacg tacaataaac ccgattccaa cccatccggt atgcctttgt
    56941 gaaccagctt attttcgact ttgccgcaca cgactatccg agtttcgaca aattcctcgg
    57001 cacggaaaat gccgaactgg tctatgtgtt acagcataaa cacgacccgt ttatctacgt
    57061 ctggggcgaa gaaggcgcgg gcaaaagcca tctgttgcag gcgtgggtcg cacaggcgct
    57121 cgaagccggc aaaaacgccg cctatatcga tgccgcctcc atgccgctga ccgatgccgc
    57181 gtttgaggca gaatacctcg ccgtcgatca agtcgaaaaa ctgggcaacg aagaacaagc
    57241 cctgcttttt tccatcttca accgcttccg caacagcggc aaaggctttt tgctgctcgg
    57301 ttcggaatac acgccccagc agctcgtcat ccgcgaagat ttgcggacgc gtatggcata
    57361 ctgcctcgtt tacgaagtca agccactaac cgaccaagaa aaaatcgacg cgctcgccaa
    57421 tatggcggcg gcacgccaag taaccgtcga ttccgaaatt ttcgaatacc tgctcaaaca
    57481 ctggcggcgc gatatggaca gcctgatgat gatgctcgac acgctggaca actacgccgt
    57541 aaccatgggc aaacgcatca ccctgccgct gcttcgccag cttttgaaac aacaggaaac
    57601 ccaatgaaaa acctcgccat cttcgacctc gacaacaccc tcatcaacac cgattccgac
    57661 cacgcctggc cgcaatacct catcaaaaaa gggttggttg acgctgccga aaccgaagcg
    57721 caaaacgaaa aattctaccg cgactaccaa aacggctgcc tcgacatcga cgcattcctc
    57781 aaactccacc tcgccccgct cgcccgttac agcaaagaag agctggcgga gtttcaccgc
    57841 gaatttatgg cggaatacat catcccccac atctcgccta tgcagcgtat gctggtgcag
    57901 agccaccaaa tggcaggcga cgaaaccctc gtgatttcct caaccaacga gttcatcatc
    57961 acccccgtct gccgcctttt cggcatcgcc aacatgatcg gcacacaact cgaaaccggt
    58021 cccgacggcc gctacaccgg caattacatc ggcacgccca gcctcaaaga aggcaaaatc
    58081 acccgtctga accaatggct cgccgaacgc ggcgaaacgc tcgaaagcta cggcaaaacc
    58141 tatttttaca gcgactccaa aaacgacctg ccgctgctgc gcctcgtcag cgaacccgtc
    58201 gccgtcaacc ccgatgccga actggaaaaa gaagccaaag aaaaaggctg gccggttttg
    58261 aatttcaaat gacgcaatgc cgtctgaagc ccgattcggc gttcagacgg cattgaggct
    58321 gaaatcccaa aaacccaaag ccacaggaat tcatccggaa aaacaaaaaa cacaggaatt
    58381 catcgggaaa aacaacaatc tttccaccgt cattcccacg aaagtgggaa tcttgatccg
    58441 tccgcacgga aacttatatt tcgtcattcc accaatccca caccaccttt accggttcaa
    58501 atccgcacaa aaatccacat cccgtcattc ccacgaaagt gggaatctag gacgtaaaat
    58561 ctaaagaaac ctttttttcc cgataagttt ccgtgccgac aggtctggat tcccgcctgc
    58621 gcgggaatga cgaaatttca aagttatggc gttatcggaa aaacagaaaa tcaaagccgg
    58681 agaatttatc caaaacaacc ggatttcaaa aaaaccaaat tcccggcggg aatgacggat
    58741 tttaggtttc tgttgtggtt tctgtttgct cgggaataac aacttagaaa ttgcccgaaa
    58801 ccccaaaaac agaaaccgga caaacaggtt ttccgccaaa gccggcattt tccgactttg
    58861 cttcaggcat cttcccctct gcctaaaatc atcgacccga taccggcatc ggtaaagatt
    58921 tccagcaaaa gcgcgttggg caacctgccg tcgatgatgt gcgtggcttt cacaccgttg
    58981 gcggcggctt cgaccgcaga agcgattttc ggcagcatac cgccatacag cgtgccgtcg
    59041 gcaatcagcc catcaatccg tttcggcgtg agtttggtca gcagattgcc cgttttgtcc
    59101 atcacaccgg cgatattcgt catcatcaag agtttttcgg cgttcaattc ttccgccaat
    59161 ttccctgcca ccaaatcggc gttgatgttg aacgcttcgc ctttttcacc tacgccgacg
    59221 ggggcgacga cgggaatgca gccgcgttct atcagccctt taaccaaacc ggtatcgatg
    59281 ctttccaccg taccgacctg tccgatgtcc acgctattct gttcgggcgt atcgaccaaa
    59341 agtttcttcg ccttaatgaa atggtcgtcg cgcccgctca cgccgaccgc gtgccctcca
    59401 tatgtgttaa tcatcgacac gatttccttg ttgacgtgcc cgcccaatac catttcgaca
    59461 atatccatcg tctctttgtc ggtaacgcgc attccttgga caaattcgcc ctttttgccg
    59521 actttttcaa gcatcgcatt gatctgcggc ccgccgccgt gaacgatgac gggatgaatg
    59581 ccgaccagct tcagcagcac gacatcgcgg gcaaaccctt ctttcaaggc aggttcggtc
    59641 atcgcgttgc cgccatactt gatgacggcg accgaaccgg aaaaccggcg gatgtaaggc
    59701 agtgcttcgg caaggatacg cgccttgtcg gcggcggaaa taatgttttc aaactccata
    59761 acggctccat attgttgaca atttcggctt atgttaacgc caaatgccgt ctgaaacaat
    59821 cttcagacgg cgtttggcac accttcgggc aggttgaagc cttgcgatac gggcggtaaa
    59881 atcgcggttt atccggttcg agaaaccggc gacagcaagg actttcccat gattaaaatc
    59941 agcacccgct tcgatgccgg ctcggtcgtc gtcaaagacc tgaccgaccc ttccaacatc
    60001 cgcctcgccc tgcgtcccga caacgcctcc gatttcgcac aatggttcta cttccggctg
    60061 caaggcgcgg cctatcaaaa ctgcatcatg cattttgaaa acgcggcaga agccgcctac
    60121 ccgaaaggct gggaaggcta tcaggcgtgt gcctcatacg accgccgcaa ctggttccgc
    60181 gtaccgactt cctacgaaaa cggcgtactg accgtcaatc atacgccgct gtccaacagc
    60241 gtgtattacg cctatttcga accctattcc gaagagcagc acctcaacct cctcggcgac
    60301 gcacaaggca gcggcctgtg ccgcatcgac gacttgggca gcaccgtgca aggccgcgac
    60361 atcaatctgc tgaccatagg caaccaagtc gaaagcgaca tgaaaatctg gattaccgcc
    60421 cgccagcacc cgggagaaac catggcggaa tggtttgtcg aagggctgct cggcaggctg
    60481 ctcgattcgc aagacccgac agcacgcacc ctgctcgacc gcgccacgtt ctacatcgtc
    60541 cccaatatga atcccgacgg ttcggcactg ggcaaccttc gtaccaacgc cgccggtgcg
    60601 aacctcaacc gcgaatggga aaacccgact ttggaaaaaa gccccgaagt gttcttcgtg
    60661 cgcggaaaaa tgctggaaac cggcgtggat ttgtttttgg acatccacgg cgatgaaggt
    60721 ctgccctttg tctttgttgc aggaacggaa ggtgtgccga actacaatcc gcgcatttcc
    60781 gcgttggaag cgcagttcaa aaccgccctt ttgaacgcca gccccgattt ccaagacgaa
    60841 tacggttacg aaaaagacgc gccgggcaaa gcgaatatga ccttggcgac caactgggtc
    60901 ggcaaccgtt tcaactgcct tgcctacacg ctggaaatgc cgttcaaaga caacgccaac
    60961 ctgcccgacg acgacttcgg ctggaacggc cagcgttctt tgcggcttgg ggaagcggtg
    61021 ctttccgcca ttttgaacgt cgccggcgat ttgcgctgac actccccact gaaaatgccg
    61081 tctgaaagcc cttttgcgtc tttcagacgg catttgaaaa cgaaagcccg ttatgattat
    61141 ccatcaccaa ttcgatcccg tcctcatcag tatcggcccg cttgccgtgc gctggtatgc
    61201 cttaagctac atcctcggat ttattctttt tacctttctc ggcagaaggc gcatcgcgca
    61261 aggcttgtcc gtttttacca aagaatcgct cgacgacttc ctgacatggg gcattttggg
    61321 cgtgattttg ggcggacgct tgggctatgt cctgttttac aaattctccg actacctcgc
    61381 ccatccgctt gatattttca aggtatggga aggcggaatg tcgttccacg gcggcttttt
    61441 gggtgtagtt attgccatat ggttgttcag ccgcaagcac ggcatcggct tcctcaaact
    61501 gatggacacg gtcgcgccgc tcgttccgct gggtctcgct tcgggacgta tcggcaactt
    61561 tatcaacggc gaactttggg gacgcattac cgacattaac gcattttggg caatgggctt
    61621 cccgcaagcg cattacgaag atgccgaagc cgccgcgcac aatccgcttt gggcagaatg
    61681 gctgcaacaa tacggtatgc tgccgcgtca tccctcgcag ctttatcagt ttgcccttga
    61741 aggcatctgc ctgttcgccg tcgtttggct gttttccaaa aaaccgcgcc cgaccgggca
    61801 gactgccgcg ctttttctcg gcggctacgg cgtgttccgc tttattgccg aatttgcgcg
    61861 ccaacccgac gactatctcg ggctgctgac cttggggctg tcgatggggc aatggttgag
    61921 cgtcccgatg attgttttgg gtatcgtcgg ctttgtccgg ttcggcatga aaaaacagca
    61981 ctgaccgcct gtgaaaaata ccgtctgaaa catggaaatc cggtttcaga cggcattttt
    62041 acgtttggtt cagatgaaaa caaacgcttc aattcttacc gcaacacgcc ttgtatttcc
    62101 tgccgctgcc gcacggacag ggatcgttcc tgccggtttt ttccccttcc ctgcggacgg
    62161 tttgcggttt gttgatgacc gcctgccagt agcggtagat gtctgccagc gcgtaaggca
    62221 gttcggacgc aagttccgcc agctcgcctt cggtgaattg caggcggata gcgccgtttt
    62281 cctcttcgtc gtaaatgccg cccaatgcca tcacggggta aaacagctct tcaaacgctt
    62341 catcatcggc ggcttcaaac caatcggtcg gcacaatgtc caaaccgtaa agataggcgt
    62401 tgcaccaagt gtaaaaatcg ctgccgccct cgccgtcgtc gtagagccac aaatcgggca
    62461 gctttttatc cgacatcgcg gcggttgttt ccatcgccat tgccaaaacc agccgttcga
    62521 tttcggaacg ttcggcggcg gtaaattgcg attcgtcgcc caacacttcg ggcagccagt
    62581 cgagcggtgc caatttgtcc ggcccgctca acagcgccgt cataaaacct tgaacctcgt
    62641 cgcaacgcat cgtgttgcct tgttcgcttt tggcatccaa caattcgccc aaccgccgtt
    62701 tggatgcttc ggtaaatttt cgggaatcca tcattttcct tttcaaatgg gttttgcgcc
    62761 ctattatagt ggattaaatt taaatcagga caaggcggcg agccgcaggc agtacaaata
    62821 gtacggcaag gcgaggcaac gccgtaccgg tttaaattta atccactata tcgccgcaat
    62881 gccgtctgaa gcctttttat gcttcagacg gcatcgtctg ttttcacgct caaatcgtac
    62941 cgctgccctg cgccttcggt ttggcggcac taaattccag cttgctcaaa gcggaaaggt
    63001 aagctttggc ggtcgccgcc aaaatgtcgg tatccgcgcc ctgaccgttg gcgacgcggt
    63061 tgccgcgcgc caaacggacg ctggtttcgc cttggctttc cgtaccttgc gtgacggcgt
    63121 tgacggaata aatctgcaaa gtcgcgccgc tttgcgccac gctctcaatc gctttgaaaa
    63181 ttgcatcaac agggccggag ccggttgcgg aagcgcgttt ttcttcgcct ttgatgccga
    63241 acacgatgtc ggcgcgcggc tcttcgccgg tttcggtgct gattttttga gaaatgaatt
    63301 tatagctttc ggcattcata ttgcccattt cgtccgacac cagcgcgtgc aggtcttcat
    63361 cgaagatttc gcgttttttg tcggcgagtt ctttgaagcg tgcaaatgcc gcgttcagtg
    63421 cctcttcgct ttccaactcg atgcccaaat ccgccagctt ggttttgaag gcgttgcggc
    63481 cggacaattt gcccaagctc aatcggtttg ccgaccaacc gaccgattcg gcagacataa
    63541 tctcgtaagt ttcgcggtgt ttcagcaccc cgtcctgatg gatgcccgat tcgtgcgaaa
    63601 aggcattcgc gccgacaatg gctttattgg gctgcacggg atagcccgta atggtggaca
    63661 ccagtttgga cgaaggcacg atttgcgtgg tatcgatgcc ggtttccaag ccgaacaagt
    63721 cgtggcgcac cttcaacgcc atcacgattt cttcaaccga agcattcccc gcgcgttcgc
    63781 ccaaaccgtt aacggtacat tccacctgac gcgcgccgcc ttttaatgcg gcaagcgaat
    63841 tggcaaccgc caagcccaaa tcgttgtggc agtgtgccga ccagacgact ttgccgccgt
    63901 tgggcgtttt ggcaatcagt tcgcggaaaa attcttcggt tttgtacggg atggaatagc
    63961 cgacggtatc gggaatattg atggtggtcg cgcccgcttc aatcaccgcg ccgcagattt
    64021 cggcaaggaa atcgatttgc gaacgcaacg cgtcttcgca ggaaaattcc acatcgtcgg
    64081 tgtattcacg agcgattttt accgctttga ccgccgcctc aatcacctgc ttcggcttca
    64141 tcttcagctt gtactccata tggatggggc tggtggcgat gaaggtgtgg atgcgttttt
    64201 tcggcgcggg cgcaacggcc ttacccgcct gacggatgtc ccgctcgatg gcgcgggaca
    64261 atgaacagac cgttgatttg gtaatggttt tcgcaatcgc attgaccgcc tcgaaatcgc
    64321 ccgggctggc ggcggcaaaa cccgcttcga tgatgtccgc gcccaatttt tccaactggc
    64381 gggcaacgcg gattttttcc tctttggtca tagcggcgcc gggcgattgt tcgccgtcgc
    64441 gcagggtggt gtcgaaaata ataacgcggt tggcctgtgt catttctgtt ctctccagag
    64501 attcgttttt ttgtaaaaaa gcattcaaat ccagcggtct tccttgcgat tcggcaagcg
    64561 cggtcaattt ttgcagttgc gccaaaggca gcgagccgcg tgtgcgccat ttgtagatgg
    64621 cggcggtcgt cgcggcattt tcaggatcgt gccgtttcaa cgcttcggca agcgcgttca
    64681 cgccgccgaa ataggcgacc agacggtcta tatctaattg catggtagtc cttgtctaaa
    64741 ataaatcagc ccgcagcaga aatatggaca ggattatacg cattttggac aaaacggcaa
    64801 tattaatttg aaaaaatctc aataagttgc tataaatttt attaatttta tacattttgt
    64861 ctattttatt gcagatgcgg cgtgattgtt gacaattgcg acagaacagg gcaaattcgg
    64921 cggtacaagg cggcaacgag acaggacaaa ccaacaagga cggaaacaat gaaatacaaa
    64981 agaatcgtat ttaaagtcgg cacatcttcg attacccatt cggacggcag cctctcgcgc
    65041 ggcaaaatcc aaaccatcac ccgccagctt gccgcattgc atcatgcggg acacgagctg
    65101 gtcttggtgt cttccggcgc ggttgctgca gggttcggcg cgctgggttt caaaaaacgt
    65161 ccggtcaaaa tcgccgacaa acaggcttcc gccgccgtcg ggcaggggct gctgatggaa
    65221 gaatatacgg caaacctgtc ttcagacggc atcgtgtccg cacaaatcct gctcagccgt
    65281 gccgactttg ccgacaaacg ccgctaccaa aatgccggcg gcgcactttc cgtgctgctg
    65341 caacgccgcg cgattcccat catcaatgaa aacgacacgg tttcggttga ggagttgaaa
    65401 atcggcgaca acgacacatt gagtgcgcaa gtggcggcga tgatacaggc agacctcttg
    65461 gtgctgctga ccgacataga cggtctttac accggcaacc cgaacagcaa tcccgatgcc
    65521 gtacggctgg acaaaatcga acacatcaac catgaaatca tcgaaatggc gggcggctcg
    65581 ggttcggcaa acggcacggg cggtatgctg accaaaatca aagcggcaac catcgccgcc
    65641 gaatccggcg taccggtgta tatctgttcc tcactcaaac ccgattcatt ggccgaagcc
    65701 gccgaacatc aggcggacgg ctcgtttttc gtcccccgtg ccaaaggttt gcggacacag
    65761 aagcaatggc tggcgttcta ttccgaaagc cggggcagcg tttatgtgga cgaaggtgcg
    65821 gaacacgctt tgtccgaaca agggaaaagc ctgctgatgt cgggcattgc cggaatcgaa
    65881 gggcattttt cccgtatgga caccgtaacc gtgtacagca aggcaaccaa acagcccctg
    65941 ggcaaagggc gcgtcctttt cggctccgcc gccgccgaag acctgctcaa atcgcgtaag
    66001 gcgaaaggcg tgttcatcca tcgggacgat tggatttcca tcacgcccga aatacgcctg
    66061 cttctgaccg aattttagaa aaggaaaccc atgtcaaaca cacaaaaaca gcttgccctt
    66121 gccaaagcgg caaaaaaaat ccgtcaacac ggcggatgcg gaagaaaaaa accgcgcgct
    66181 gcttgctatg gcggacagtc tggaagcggc ggcggaagat attttggcgg caaaccgttt
    66241 ggatttgaaa gcggcggcag gcaaaattcc cgacagcatg accgaccgcc ttttgttgga
    66301 cggcaaacgc atttgcgcga tggcggacgg catcagggcg gttgccgcgc tgcccgaccc
    66361 cgtgggcgaa atactggaaa cctcgactct gcccaacggc ttggaaatcg tcaaaaagcg
    66421 cgtggcgatg ggcgttatcg gcattattta cgaaagccgc ccgaacgtta cttccgatgc
    66481 ggcggctttg gcactgaaaa gcggcagcgc ggtcgtactc cgcagcggca aagatgcatt
    66541 tcaatccgca cgcgccatcg ttgccgccct gaaaacgggg ttggcacaaa cccgcatcga
    66601 ccccgaagcc gtacagctca tcgaagacac agggcgtgag ggcagttacg aaatgatgag
    66661 ggcgaaagat tatctagacc tgctgattcc gcgcggcggg gcagggctga tacgggcggt
    66721 ggttgaaaat gccgtcgtgc cggtcattga aacggggacg ggcatcgtcc acatttatat
    66781 cgacaaggac gcggattggg acaaggcact ccgtatcgtg tacaacgcca aaaccggccg
    66841 cccgtccgtg tgcaactcga tggaagtgct gctggtgcat gaaggcattg ctgccgactt
    66901 cctgcccaag ctcgaacggc tgttggttcg cggccgtata gaagccggac tgccgcccgt
    66961 ccgcttccgt ttggatccgc aggcggcgcg gtatatcggc ggcgaagcgg cgggtgcaga
    67021 cgatttcgat accgagtttt tagactacat cctcgccgtg aaaaccgtcg cttcggttga
    67081 agaggcggtc gggcacatcg aagcccgcgg cacgcaccat tcagacggca tcgttaccga
    67141 aaaccgccac gctgcggact atttcacgac ccatatcgat tctgccgccg tgtatatcaa
    67201 cgcgtccacg cgcttcaccg acggcggcga attcggcttg ggttgcgaaa tgggcatctc
    67261 cacgcaaaaa ctccacgccc ggggcccgat gggtttgaaa gagctgacga gttacaaata
    67321 catcgtacaa ggcacgggac aggttaggga ataatccgaa ttcaaacgtc tgatatagtg
    67381 gattaaattt aaaccggtac ggtgttgcct cgccttgccg tactatttgt actgtctgcg
    67441 gcttcgccgc cttgtcctga tttttgttaa tccactataa aaatgccgtc tgaaacagtc
    67501 ttcagacggc atttctattt gcaaaaaaat caagcattgt ccaaggggac gagaatcgta
    67561 cggttgccgt tgtgttccgg tgcggacaca atgccttccg cctccatctg gtcaatcaga
    67621 cgcgcggcgc ggttgtagcc gatgcgcaag gcgcgctgta cgcccgaaat gctggctttg
    67681 cgcgttttca ggacaacgga tacagcctcg tcgtacatcg gatcggtttc gccgtcgccg
    67741 ctgcgcccga taccgggcag ctcttcgctg ccgccgccgc tcaaaatatc gtcaacatag
    67801 tccggctcgc caaactgttt caaatattcg accacgcggt gcacctcttc atccgaggca
    67861 aacgcgccgt gaacgcgttg cggataggca gtacccggcg gcaggaacag catatcgccc
    67921 tgaccgagca ggttttccgc gcccatttgg tcgagaatcg tgcggctgtc gattttgctg
    67981 gacacttgga acgcgatacg cgtcgggatg ttcgccttaa tcagacccgt gatgacatcg
    68041 acgctggggc gttgtgtggc aaggataagg tggatgcctg ccgcgcgggc tttttgggcg
    68101 aggcgcgcaa tcagttcttc gattttcttg cctgccgtca tcatcaaatc ggcaaactca
    68161 tcgaccacga ccacgataaa cggcagtttt tccaaaggtt cgggatcgtc gggcgtgagg
    68221 ctgaacggat tgccgatttt ttctcccctt gctgcggctt cggcgatttt ttggttgaag
    68281 cccgcaagat tgcgcacgcc cataaagctc atcaggcggt agcgtttttc catttcgtta
    68341 acacaccagt tcagcgcgtt tgccgccagc ttcatatcgg taacgacagg ggcgagcagg
    68401 tgcgtgatgc cttcgtaaat gctcaattcc agcattttcg gatcgatcat aatcatacgc
    68461 acgtcttccg gcgcggcttt gaaaagcatg gacagaatca tcgcgttgac acccaccgat
    68521 ttgcccgaac cggtcgtgcc ggcaaccagc aaatgcggtg ctttgcccaa gtcggttacg
    68581 acgggctgtc cggtgatgtc ctgaccgagc gcgagcgtca gcttggattt ggattcggca
    68641 aactcgggcg aattgaaaat ttcgctcagg cgtatcattt ggcgtttcgg gttcggaagt
    68701 tccaaaccca tgcaggtttt gccggggatg gtttcgacaa cgcggatgga agccacgccg
    68761 agcgaacgcg ccaagtcttt ttccaaattc agaacggaat tgccgcgcac gccgacatcg
    68821 ggttcgattt cataacgcgt aatcacgggg ccggaataag aatcgacaac cttgaccttg
    68881 actttgaact ccgccaattt ttcttcgatg gtgatgctgt tttccaacag ttcttcttcg
    68941 gtttgcgtcg cctcgggatt gaacagcgga ggcagaagca ggtcggttgt cggcaggtgt
    69001 tcggataccg caccggtctc ttccgattgg aacgcccctt catccgcttc cgtatccgat
    69061 acccggcagg acgggcgttc agacggcaca tcttcaaacg gacaaaccgc ctgactgtca
    69121 tgcccgaacg cttcggtttc cgacagatat tgcccgcttg accgctctgc cgcaccctca
    69181 ctgccgtcat ctgcaatagc ggcggtttcc tcctgccaac ctccattcaa aacatcagcg
    69241 gcaagatggt cggtttcggc aatgcggctg cgttgcgcct gctcgaatcc tgccggcggc
    69301 tcataggtac ggttgtagat ttccgatacg ggaggcggcg gcagaatatc gatttcgggt
    69361 acggctacct ccggcacttc gggtggttcg accacccaag catcgggcgc ggcaggctct
    69421 tcaatatgga tatcggcagc ttcatcataa accggaccgc cgtatcccac agacgaaacg
    69481 gtttccgtga aaacattttc ggtttcagac ggcatttcga catcccgttt cccgacaacc
    69541 gtccgacttt ccggaatcag gcgcgaaatc cgcgcctccg cagaaaccgt tgccttgttc
    69601 ggttctttca aattgacgga aacgcggcgg cgggcatcgc ggacggctgt gccgtctgaa
    69661 acggactgcc cttgggaaac atccggggat tttgcctccg cctgcccttt ctcctgccct
    69721 gcatgacggc ggaacggcgc attccgtgca ttttcggctt tgactgcaga aaaggcagac
    69781 ggatgctgct tctcaaaata cggctcaaat ccgtaatccg cagactcgga aaacgcttct
    69841 ttgtccgcat caaacatacg ggaatacgtg cgttgaagca cagggtcgtc caaaccgata
    69901 atctgcagcc cttccatcgg ggtatcggac acgcgtactt tggggacggc tgttccgttt
    69961 ttctcaaatg catcgatata gcgttttttc gtttccctta aagccgcgct gcttaaagca
    70021 cgcgttgctt cttccaaagt gatttcctta aaaaccggac ggacgggcga agttttgctt
    70081 tcagactgca tcaacccttc agcaatactc cggtcgaatg ggatgcggcg gttgtcgatt
    70141 acggcagttg caatgtcttc cgtatcggca gcctcttcct ccgcagcttc tgctgcttcc
    70201 gtttccgctt cttccgtccc gtttccactg tctgaatacc cgtcggatgc ggtcggaaca
    70261 tccgcacggt ttgcagaagc aggcggaacg ggcctgtccg gttcggattc gtgcgccaga
    70321 tagtttcggc agaaacggac ggcaccgaca aacagccaat acactgccgt tttgacggca
    70381 tggaaaagca tcagggaaaa ctcgggaaaa tcgggcatac cgtctttgat ttcaggcagc
    70441 tccgcctgtt tttccccttt cttttcctgc cacgcagaaa cctcgcgcat ccactcgcgt
    70501 tcggattgtg cgcggacaaa aaacaggccg gcaagcgcaa gcaacaaaat aacgatcaaa
    70561 actatccaaa acatatgccg tttcccgcaa gacaaacgat taaccgagtt tattttaacg
    70621 tgtttccagc ccaaaaaaaa caaccggccg gcaattctgc cgaaaccgat tcggctttcg
    70681 gtttgccccc gccgacagta taatagccgc cccttccctt aatctttgcg gcattaccat
    70741 gattttccaa acagtctggt tttcagatgt ggtactgtcg gtttcgtgga ttgtccttat
    70801 cctgatactg gcagcttccg cgccgtcggc attccgttcg cttgcccgct accgttccgc
    70861 ccttcccctg tgtaccgtaa ttttttccgc agcatggtgt ctgaacgcct ctgtcggcgg
    70921 cggacagctt gcccagatga actaccacct gcttgccgtc aatctggtta cactgatggt
    70981 ggatacctcc gccgcccttt ggcttgccgc gctgctgatg ctgccctact gcctgctgtt
    71041 tgccggttct gccggcgcat atccgccgaa cgctttggtg ctgatactgc ccgcgcttgt
    71101 cgtcaaccgc ctgtcgcgta tgctggtcaa ccgtctgccg cccaatattt tcatctttat
    71161 tttcgtcaac ggctttctcg cttccgccgc cggcattctg ctgaccgggc tggtgctgat
    71221 cggtattttg gatgccgcaa acgcttttcc gtccgaaata ttgtggacga ccgccctgcc
    71281 cgtctttatt ctgctggcgt gggcggaagc cttcctcagc ggcatttcaa ctgccatttt
    71341 cgtggcactg aagccccatt ggatcaacac tttcgacgac aaccgctatc tgaagtccga
    71401 gcgcggcata tggctgtagc cttcagacgg cataggcaga caaaaccatg ctttccaaca
    71461 tcctccccct ttccatcggt gcaatattcg gcacaacggc gcgttggctg ctcaacctcg
    71521 ccgtccccgc atcgttgtct cccgccacag gcaacctgtt tgccaactgg acgggcgcgc
    71581 tgctcatcgg catctttgcc gaaaccgtca gccatccgca atggaagctg ctgctgatta
    71641 ccggtttttt cggcagcctg accacgcttt ccggattttc actggaaacc gtaaccctgc
    71701 tccaatcgaa ccgtcccgct tcggcacttg ccaatatttt cctacacacg gcaggttcgc
    71761 tgctgctgac ttggctcggg ctgaagatag ggacggcagt caaataaccg caaaaaattg
    71821 cttcctttgg ttttgacata atctataaat tggcttcgct tgtttaaata ctgcctgtgc
    71881 caattatagt ggattaaatt taaatcagga caaggcggcg agccgcagac agtacagata
    71941 gtacggcaag gcgaggcaac gccgtaccgg tttaaattta atccactata ccgttctcaa
    72001 attcaaattg ggcttttagg tctataacaa agaaaaagcc ttgggaggat aaacctacca
    72061 aggctttttc ttaaaaaacg ctctatcact gtgcctaaaa atgcaaaaaa gtaaccgtca
    72121 taatcctttt gccaaaatca atatcatcat ccgatgaatg aacggacggt atataagaaa
    72181 gcgttaataa actcataaac cagtaagaaa aatcagtaac gaagatattc attattaccc
    72241 attcttaaaa attatcacgt tcgtttgggt aattatcata cccttttccc tcttattact
    72301 aaagcacgaa ttactttaaa gtacgcaccc agagacaagc cgaaaaggca tatccgatcc
    72361 gccaaacaaa atgcccgccg acttactccg gcgggcggtt ccaacatcct gtcaatcctt
    72421 catcagccag tattgcaaac cgattaatgt tttaccgtct ttaatttcat cgtttgccaa
    72481 tgcctgacgg acttcatctt tcgacatcag cacggtttcc gttatttcat cttcatcatt
    72541 agaaagcgta ctgcccaagc gcacgccttc cgcctcgaac agatacattt tttcattgca
    72601 aaaaccgacc gccgtataaa aactgtacag caggcgcacg ctgtcggcaa cataaggcgt
    72661 ttcctccgcc aattctcgca gcgcacacgc tgccatatcc tcgcccgcca catccagctt
    72721 gcccgcagga agttccaatg tcgcctgatt tgccgcataa cgccactgcc gcaccaaaac
    72781 gaccttttcg tcatccgtta ccgccaacac gcacgccgcg cccggatgcc ggatgacgat
    72841 gcgctgccct tcattgccgt tgggcagcct gaccttatcc ctgctgatac tgacgaaacc
    72901 gccctcgtaa atggtttcgc cgcctaattt tacttccctc aaatccactt catatctcct
    72961 ttccgtttcc tattctgccg ttcaatcttc acgcttgccg cgctcgattt ccactccaac
    73021 ctcgcgcacg ccttccaaaa tacccggctt gacgattttc acgtgcaccc acaccgcgcc
    73081 gaaatatccc aaaaccaaat cggcaatata ttccgccaac gcttccaaaa gcaggaaatc
    73141 ctgttctttc agatgtcggc gcagcgtttc gcatacctcg gcataatgca ccgtattggc
    73201 aatatcgtcg tccgaacccg ctttctcggg aacgccgatg tccaaatcga caatcagggt
    73261 ctgcaaccgt tcgcgttccc agccgtacac gccgataagc gtatccgcct tcatgccgtg
    73321 caaaaagatt ttatccataa tccgccccgt tttttgctaa aataaccgca ccattctaag
    73381 taaagtaccg caaatgttca acataccggc cgtcgccgtt tcctatttaa tcggttcact
    73441 ttctttcgcc gtcatcgttt ccaagtatta cgggatggac gacccgcgca cctacggatc
    73501 gggcaatccc ggcgcgacca atgttttacg cagcggcaaa aaaaaggcgg ccgcgctgac
    73561 gctcttgggc gatgccgcca aaggtttggt tgccgttttg cttgcacgcg tgcttcaaga
    73621 accgctcggt ttatccgaca gcgcaatcgc cgccgtcgca ctcgccgcgc tggtcgggca
    73681 tatgtggccg gtgtttttcg gatttaaggg cggcaaaggc gtggcaacgg cattgggcgt
    73741 gcttctggca ctctctcctg caactgcctt ggtctgcgcg ttgatttggc ttgtgatggc
    73801 attcggcttc aaagtatcct cccttgccgc gctggtcgcc acaaccgccg ccccccttgc
    73861 cgcactgttt tttatgccgc atacttcttg gattttcgca accctcgcaa tcgccatatt
    73921 ggtgttgctc cgccataaga gcaacatcct caacctgatt aaaggcaaag aaagcaaaat
    73981 cggcgaaaaa cgctgattcg gcatttgttt tccattacgt catgccgtct gaaaccatgt
    74041 tcccgcgttc agacggcatt tccgcacaaa cggctttcat acataccatg ctcagttaca
    74101 gacacgcatt tcacgccggc aaccacgccg atatgctcaa acacttcacc ttgtttttag
    74161 tgctgcaata tttcaaccgc aaagacaagc cctactggta catcgacacg cacggcggcg
    74221 cgggtttgta caatctcgaa ggcagcgagg cgcagaaagt cggcgaatac cggcaaggca
    74281 tcgccctgct ccgacaggcg caaaacctgc ctgccgaact ctctgacttt gccgcgcaca
    74341 tacaaaaaat cctaccctcg cccgaacttt actgcggctc tccgtggctg gcgcaatcgc
    74401 tgatccgcat cggcgacaaa ttgcgcctgt ttgagctgca ccctgccgac tttgtccatc
    74461 tgcaaaacaa tatgggcgaa gcgggattgg gcaaacgcgg acaagtgttg cgcgaagacg
    74521 gttacaaagg gctgatttcc ctgctgccgc ccccgccgcg acgcgctgtt attctcatcg
    74581 acccgcccta tgaggaaaaa caggactacc ggcgtgtaac ggaaacgctg aaagccgctt
    74641 taaaacggtt tgagtccggc tgttatctca tttggtatcc ctgcctcagc cgcgaagaaa
    74701 gccgcaagct gccggaagaa ttgaaaaaac tcctgcccga caactacctt tacgccgaac
    74761 tgcacgtcca cgcgccgaaa accgacggtt tcggtatgca cggcagcgga atgttcgtga
    74821 ttaatccccc ttacctttta gccgaacaat tggcggcaaa cctccccgcc ctgacacggc
    74881 tgctggcgca agacgaaggc gcgcgctacc tgttggacaa caaaatccgc tgaccccgat
    74941 gccgtctgaa acccttcaga cggcatcttc cgtcaaacaa acaaaaccgg gcgatacaca
    75001 ctccatatcg tccggttctg cataaaacca gtattcaaaa atcagaacac gcttttcatt
    75061 acacccacgg taccaaccac accgttcagc agcatcagac caccggcagc cgtacccata
    75121 ccttgacgga ttgcgtcatg cttattggca cgtttttcac gttccaaagc caactcctca
    75181 gaaacatttt tacgttgacg gcggtgttgc gccagttccg cacggctaat tcgcgcttcg
    75241 gcacggttac gaccagcctt gattacggcc gaatcgtcgc cgatagcata gttggctaca
    75301 gaattttcag aagcgcaggc agcaagcaaa acggcacaca cagcaacaac ggaaatattt
    75361 ttcaagtttt tcatacctgt cctattatct caaaataaag aaaaaactaa tccaactgga
    75421 gttttatcgg ataaaataaa tattgggtga attttcgttt gcctttttga tggcgcgtgc
    75481 cgcatccatc atttcttccc ggcgttcttt acggtattcg gcacgctcgg cacgctcgct
    75541 tatctgaatg gcacgacttt tcgcctgtat ttccgctgca gattcttgag ttacaaaacg
    75601 gcggtctgcg taagtacatg ccgtcaccaa catcgcagcc accgacaaca aaatcagttt
    75661 cttcattttc atctacctat atttaagttt atttgtaaaa tatgttatca ctatatatat
    75721 atataatgtc aatatttttt aaaaataaat ttggcatctt gtacttctcg atccgcctgc
    75781 cgcgttccga cttgccccgc gccgtttttt tccactacaa tcccacgcga tatccggcga
    75841 aacgcaccaa aggaacgccc cgcagcaatc tgccgccttt cagacggcac aaacctttcc
    75901 gaaacgagca aaccatggac acactgaccc gattcctccc cgaacacctg caacaaaacc
    75961 aactgcccga agcactcggc ggcgttttgc tgtccgtcgt ttccgcctgt accgaaatca
    76021 acgccaaagt ccgcctcggc gcacttgccg gcgtattggg tatggcgggc acgggcaata
    76081 ttcagggtga agaccagaaa aaactggatg ttatcgccaa caacattatg attgacacac
    76141 tcaaagccaa ccctgccgtt gccggtttgg caagcgagga agaagacact ttcgtaagtg
    76201 ccggtgaaaa cggacgctat ctcgtcctat tcgacccttt agacggatcg tccaatattg
    76261 atgtcaacat ttccgtcggt acgattttct ccattctcgc caaacccgaa ggcgcattgg
    76321 caaccgaatc attccttcaa acgggcagac agcagcttgc cgcaggctat gtcctctacg
    76381 ggcctcaaac ccagctcgta ttcacattcg gacacggcgt gtacgtattt acactcaatg
    76441 ccgaaaacga atttgtgctg accaaagaaa acccgaaagt acccgaaagt accaaagaat
    76501 tcgccatcaa tatgtctaac cgccgccact ggctaccccc cgttcaacaa tacgtcgacg
    76561 agctcttggc gggcgaaacc ggtacgcgcg gcaaaaacta caatatgcgc tgggtagcca
    76621 gtatggttgc cgaaatccac cgcatcctga tgcgcggcgg cgtgttcatg tatctgcaag
    76681 acaaacgcga cccgtccaaa cccggcaaac tgcgcctgat gtacgaagcc aaccctatgg
    76741 ccctgatact cgaacaggcg ggcgcatcgg caagcaacgc atatcaagcc atgctcgata
    76801 tacagcccga aagcctgcac caacgcgtcg ccgtcattat gggcagcagc gaagaagtgg
    76861 attacctcaa ccgtctgcat tcaaaatagg cagagaccgt actgataaag accggcggca
    76921 gcgtgaatcc gatactgcgc cgatatttca agcataccct ccggaaaaac agaaaactgc
    76981 ctttccgaac gataaaaata ccgtctgaaa aattttcaga cggtatttta aaaattaaag
    77041 ctcaaattta tttgcccgcc gctttttgca ggatggcgta caactcgtct ttcagtttca
    77101 atttggcttt tttcagctca tcgatggttt ccgcaccgct ggtaaccgga ttgttggcca
    77161 gaccggtaat tttatcgtcc agctcgttat gctcgtcgaa cagacgggcg aagcgggaat
    77221 tttcctgttt caatttggaa atcaaatcac ggtattctgg gaacataatc gctttcctct
    77281 cttggtttac ggttaaaaag tcaaattgaa tctttacatt aattataaca aagataatat
    77341 ttttatccag tgttttgtta tacacggcaa caccgtccgc agaaaaccgc cccaccgtcc
    77401 gataaaatcg ttgacaatct tccgctttac gcataaattt tcagcccttc ctactgcata
    77461 tgaaaggcac gccccagttt ccccaatgtg gattttcagc gcgcgcagta gaggtgccga
    77521 cccaaattgg cagacccttt gcttttgtta atatcctaga aaatccagaa attcgcagta
    77581 ctctaccact catcgccaat tggccaacct ttccacggct ttgggttaat ggtgagttaa
    77641 tcggtggtag tgatattatc ctacaaatgt accaatcagg tgagctaaaa ccactcattg
    77701 aagagcatag cccaaaggtc tgattactta atatcttaaa ataaatatca aaaaagactg
    77761 ctgatgacat tagcggtctt tttttgatgg tcaagattga tagtttggat tgaattattt
    77821 agccatatta catacaataa tcagcatgct ttgtatgaat tatcaatcaa actcaggcga
    77881 aggagtgctt gtggctaaaa catatttatt gactgcattg ataatgtcta tggtaatctc
    77941 cggatgtcaa gtcatccatg ccaatcaagg taaggttaat actaattctg ctgtcatcgc
    78001 aggtgcagac gctcacacgc ctgaacatgt aacgggactg accgaacaaa agcaggtgat
    78061 tgcaagtgat tttatagtag cgtcagccaa tccattagca acacaagctg gctatgatat
    78121 cttaaagcaa ggcggtagcg ctgcagatgc gatggtggcg gtgcagacga cactaagctt
    78181 ggtagagcca cagtcgtcag gcttgggcgg tggtgcattt gtgttgtatt gggacaatac
    78241 cgccaaaaca ttgaccacat ttgatgggcg tgagacggca ccgatgcgtg cgacgccaga
    78301 attatttttg gataaagatg gttaaccatt gaaatttatg gaagcggtgg tcgctcggta
    78361 ggtacgcctg ctatccctaa actgacggag acaatacatc agcgatacgg tgtattgcct
    78421 tggggaaaat tatttgatac gccgatccac ttggcaaaac aaggctttga ggtgtcgcca
    78481 aggcttgcca tctcggttga gcaaaatcag cagcatttgg cacgctatcc aaaaacagcc
    78541 gcttattttt tgccgaatgg tgtgccgcta caagcaggca gcttgctgaa aaatttagaa
    78601 tttgctgaca gtgttcaggc gttagcagct caaggtgcaa aagctctgca tactggtaaa
    78661 tatgcccaaa atattgtttc agttgtccaa aatgctaagg ataaccccgg tcaattatcc
    78721 ttgcaaaatt tatctgatta tcaagtggtg gaacgcccgc ctgtttgtgt gacttatcgt
    78781 atttatgaag tatgcggtat gggtgcacca agctcaggtg ggattgctgt gggtcagatt
    78841 ttggggattt taaatgaatt ttcacccaat cgggtggggt atgatgctga aggtttgcgt
    78901 ctcttgggcg atgcttctag gcttgcgttt gcggatcgtg atgtatattt gggcgaccct
    78961 gattttgtac cagtacccat tcgccagttg atttctaaag actacctaaa acatcgcagc
    79021 cagctgcttg agcagtcgga taaggcattg cctagcgtat ctgcaggcga ttttattcat
    79081 gagtgggcgt cctcacaagc gattgagtta ccttctacca gtcatatatc aattgtggat
    79141 aaggcgggca atgtgctatc aatgacgact tccattgaaa atgcgtttgg ctcaactttg
    79201 atggcaaatg gctacctgct gaataatgag ctgacagact ttagttttga gccaataaag
    79261 cagggcaaac aggtcgccaa tcgtgtcgag cctggcaaga gaccacgctc ctcaatgaca
    79321 ccaaccatcg tatttaaagc aggcaagcct tatatggcaa tcggctctcc aggtggtagc
    79381 cgtatcattg gctatgtcgc taagacgatt gtggcacata gtgactggaa tatggatatc
    79441 caagatgcca tcagtgcacc caatttatta aatcgctttg gtagctatga actagaaacg
    79501 ggtacaaccg ccgtccagtg gcaacaaaca ttaaatgatt tgggctataa gactgatgtg
    79561 cgtgagctaa attcaggtgt tcaagccatt attatcgagc caagcaggct ggttggtggt
    79621 gtcgatccac gccgagaagg tcgagtgacg ggtgattaga ggtgaagctt gtattttgtg
    79681 aatttgaccg cattaagtta attattaaaa tagggggaaa ttgtgagtaa tcaaacaaaa
    79741 atacaagcca tcaagcaagc ttctgagcag atcttgacaa tttgtgagac gccaaacaca
    79801 gcattacaag ccattcatct gattttaaga catggcgggg caggaagaat tgtcttggca
    79861 agtggtgtat cagcgggtta tggctgataa agatgtggtt ggtgctggtt atttgattga
    79921 ttttgcacaa actgccgaaa atttaccatt tgatgtattg cctttgatta gccttgtgct
    79981 aaacaaaggt gatgagacgc taaaaaccgg catgttaaat aaattacctg ataatgccaa
    80041 agaaaattta cgcattatgg gctatctgcc ttaggttttg aagtattttt ttatacgaaa
    80101 aacgctcatg ataccctgat tacacaaagg agccgagaat atgttttcaa aaagcgttac
    80161 cctcgcacaa tatgaccccg atttggcagc agccattgcc caagaagacc gacgccagca
    80221 agaccacgtc gagctgattg cctctgaaaa ctacgtcagc tgcgccgtga tggaggcaca
    80281 aggttcgcaa ttgaccaaca aatacgccga aggctatccc gcaaaacgct actacggcgg
    80341 ctgcgaatac gtcgatattg tcgaacaatt ggcaatcgac cgcgtaaaag aactgtttgg
    80401 cgcagcctat gccaacgttc aaccgcactc cggttcccaa gccaaccaag ccgtatatgc
    80461 ttccgtttta aaaccgggcg acaccatttt gggtatgtct ctggcgcacg gcggacacct
    80521 gacccacggc gcgagcgtta atatttccgg caaactctac aatgccgtta cttatggctt
    80581 ggatgaaaac gaagtcctcg attatgccga agtcgaacgc ctcgcgctcg aacacaaacc
    80641 caaaatgatt gtggcgggcg cgtctgccta cgcgttgcaa atcgactggg caaaattccg
    80701 cgaaatcgcc gataaagtcg gcgcatacct ctttgtcgac atggcgcact atgccggact
    80761 ggttgccggc ggcgaatatc ctaaccccgt gccattctgc gacttcgtaa ccactaccac
    80821 ccacaaaacc ctacgcggcc ctcgcggcgg tgtgattttg tgccgtgaca atacccacga
    80881 aaaagcgctg aactcttcca tcttcccaag cctgcaaggc ggtccgttga tgcacgttat
    80941 cgccgccaaa gccgtagcgt ttaaagaagc gttgcaaccc gagttcaaac aatacgcaaa
    81001 acaagtgaaa atcaatgctg ccgtcatggc ggaagagttg gttaaacgcg gtttgcgcat
    81061 cgtttccggc cgcaccgaaa gccacgtttt cctcgtcgac ctgcaaccga tgaaaatcac
    81121 cggcaaagcc gccgaagccg ctttgggcaa agcgcacatc accgtcaaca aaaacgccat
    81181 cccgaacgat ccggaaaaac cgttcgttac ctccggcatc cgcatcggtt ccgccgccat
    81241 gaccactcgc ggctttaacg aaaccgacgc tcgcgtattg tcgaacttgg ttgccgatgt
    81301 attggctaat cccgaagacg aagccaacct cgccaaagtc cgcggacaag tgactgcctt
    81361 gtgcgacaaa taccccgttt acggaaacta aacgcccgat gtttccatac aaaatgccgt
    81421 ctgaacattt tccagacggc attttttgtt ttcggtaaca ttgctatttt ttctcttcca
    81481 gcaaaatgac caactttttc aatactgcga tttcactatc tttctgcctg atcaattcat
    81541 cctttgcatc cagcagcgac ctcaattttt cattttcaaa caccagcatt tcatcagagc
    81601 cgataatatt atgatggtta ttggtattgt cgccgataga gaagaaaaac gatcggttct
    81661 gctccgccag cagctcaacc acatcgatat tgaaaatctg ggcaatctgg cgcagcttgt
    81721 cgagattgat gccgcttttc ccgcgttcta ttttcgaata cccgttaacc gacatttcca
    81781 atttttccgc catctcttcc tgcgtccact gattgacttc ccttaacgtc cgaatcttgt
    81841 catgcacttc catcctgccc tccaaggtta attttcacca ctcaaacaac cgtaaccggt
    81901 atcttcagtt gatggtcgga tattttaaaa ataaataata taacacaata gtattttgat
    81961 aaaaatttac ctaacgaggt acagacatgt attcagaaaa caagcctaaa cgcaccgtaa
    82021 aaatgaaaaa atactggcag cctgcaccct ttccctgtcc ctttctgcct gtacttcgac
    82081 tcaagtaact tcttttgtcg agggactctc cgagggactc gtagatgtca gcgaagcctt
    82141 tatcacaaca aaggcagaat catattctgc cccgtctctt caggataagc aaattttcat
    82201 aaatcgcgtc tcagaacgag cacaaaaaaa ttacaaagaa agaaggaaaa agaaagaagc
    82261 agacttacta aattcagcat cgtcattaaa tgaatcaaca tcagaagccc tatctgccct
    82321 taaaaacgct atcgtaattc ctaaacgccc taaacgctcc ttgggcaaaa aatataaaaa
    82381 cggttttcat cctgtaaata cgtgtgcgga tgtggaagct cggtgggcaa acggagcaat
    82441 gagagatgtt agggacatag aaaggctaat aagccgaaac gtccctttac gtcgtatcgg
    82501 agacgccatt caaaacatgt gcatggaaac caatgtgtcc attaggatgt atcgccagga
    82561 gtgccgttca gaatcgtcag atttttatga cgaaatgctt gagcatcacg agaatttcat
    82621 aggatcctcc aaatcattcg catcatcact tcttcccctt agcgttacaa actttacaac
    82681 agacatggcg tgtactcccg aattttaatc taatcacact ccacaactcc ctatcaatat
    82741 aactgctact tctctgagta gtatcaacca acccttaaca aaggactaaa aaatgaaaaa
    82801 agcactgctt gcactgacta ttgccgccat ctccggtact gctatggccc agttgcccga
    82861 ctttctgggt aaaggcgaat ataccgtccg tacagacatc tccaaacaaa cgctgaaaaa
    82921 tgccgatttg aaagaaaaac acaaagtaca aaaaaacatc ggtttccgtg ccgatatgcc
    82981 gtttgacgat attcaccacg gcatgcgttt cgaagtatca cacagccgag acaaaaaaga
    83041 catgtacgtt gtgaccgaaa gtactactaa accattcggc aaagacgtta aagaaaaacg
    83101 caccgacgtg tatgccggtt acacctacac ccaaccgctc agtgaagcca ccaaactgcg
    83161 tgccggttta ggtttgggct atgaaaaata caaagatgcg gtagctaatg agaaaggaac
    83221 agtcagcacc gagcgcgaag ccttctatac caaagcgcac gccgacctga cttccgattt
    83281 gggcggcggc tggtatctga atccttgggc agaggttaag gttgacctgg acgccaaatt
    83341 gaaacacaac gcaactgtag ccggtgtctc agccgacatt aacgcaaaaa cacgcggatg
    83401 gggcgtgggc gtaggtgcga atatcggcaa acaaattacc gataccgtcg gcatcgaagc
    83461 cggcccgttc tacaaacacc gtcacttcaa agcatccggc agcttcgttt tagacggcgg
    83521 gaacatccga gtcgatccta ccaaaatcaa cgaatacggt gttcgtgtcg gcgtgaaatt
    83581 ctaatcgcgg cgatatgccc cagacacaaa accgcaagcc ttttggtttg cggtttttga
    83641 tgttgtagca gaccctaata ccgtctgaaa cttttcagac ggcatttttc caatcggtta
    83701 aaataccgcc tcgacattgt gttttggaaa acccgtgctt gcttctgtga ttgattttat
    83761 cctgcatatc gaccaacacc tgctcagcct gtcggcgcaa tacggtgtgt ggatttatgc
    83821 gattctgttt ttgattgttt tttgcgaaac cggtctgatt gttacgccct tcctgcccgg
    83881 cgattcgctc ttgtttgccg ccggcgggat tgccgcgctg ggcggtatgg atattcattt
    83941 gatggttgca ttattaagcc ttgccgccat actcggcgac gcgctcaatt ttaccgtcgg
    84001 caaatatttc ggcggcaggc tgttcgccaa ccccgattct aaaatcttcc ggtgcgaata
    84061 cctcggcaaa acccgccgtt tttacgaaaa acacggcggc aaaacgatta tcatcgcgcg
    84121 cttcgtgccc atcgtccgca ccttcgcccc cttcgtcgcc ggtatgggaa aaatgcacta
    84181 tgccaaattt atccgctaca acatcatcgg cggcttgttg tgggttatct tgttttctta
    84241 cgcgggctat ttctttgcca acttccccgt cgtaaaaaac aatctgggct tggtgatggg
    84301 cggcatcatt attgtttccg tcctgcccgg catcatcgaa atcgcccgcg ccaaacttgc
    84361 ggcaaaatcc gaacgttaat tgttggaaat tgacattatg aatatattat ccgtagaaaa
    84421 cgcttccttt gccgtcggac atgtcgccct gctcgacaaa acgtccttcc aactcgacag
    84481 cggcgaaaaa gtcggcttga tcggacgcaa cggcgcaggc aagtcttcgt ttttaaaaat
    84541 cctcaccggc gtgcaaaaac tcgacgacgg gcagattatt gttcaaaaca atctcaaaat
    84601 cgtttatgtg ccgcaggagt ccttttttga taaggacgca accgtatttg atactgttgc
    84661 cgaaggtttg ggcgaaattt gcgatttatt gcgccgttat catcatgtca gccatgagtt
    84721 ggaaaatggt tcgagtgagc tcttattgaa agagcttaac gaattgcaac ttgaaatcga
    84781 agcgaaggac ggttggaaac tggatgcggc agtcaagcag actttggggg aactcggttt
    84841 gccggaaaac gaaaaaatcg gcaacctttc cggcggtcag aaaaaacgcg tcgccttagc
    84901 gcaggcttgg gtacagaagc ccgatgtatt gttactggac gaaccgacca accatttgga
    84961 tatcgacgcg attatttggt tggaaaacct gctcaaagcg tttgaaggca gcttggtcgt
    85021 gattacccac gaccgccgtt ttttggacaa tatcgccacg cgcattgtcg aactcgatcg
    85081 cggtattttg cgctcctatc ccggctcgtt ctccaaatac agcgagaaaa aagcgcaaga
    85141 gttggcggtt gaagccgaac acaaccgcct cttcgacaaa ttccacgcgc aggaagaggc
    85201 gtggatacgc aaaggcatcg aagcgcgccg tacccgcaac gaaggccgcg tgcgccgttt
    85261 ggaagaactg cgccgccagc gtgccgaacg ccgcaatgta caaggacagg tcaacttcaa
    85321 actcgacagc ggcaagaaaa gcggcaaaat catcgccgaa ctggaacacg cttcgtttgc
    85381 ctatgacgac aaagttatca tggataaatt ttccgccatc ttgcagcgcg gcgacaaaat
    85441 cggcttaatc ggcccaaacg gtatcggcaa aaccaccttc cttaagctga ttttgggcga
    85501 attgcagccg acctacggca gaatccgtat cggcagcaag caggaagtcg cctatttcga
    85561 ccagttccgc agcgccttga atgaaaacga tactgtgttt tacacgctcg gacagggcaa
    85621 cgattacgtc gaagtcggcg gcaagaaaaa gcatgtgatg agctatttgg aagatttcct
    85681 gttcccccct gcccgcgcgc aaagtcctgt ttcttcactc tccggcggcg aacgcaaccg
    85741 ccttttgctg gcaaaactct ttactcgtcc cgccaatatt ttggttttgg acgaaccgac
    85801 caacgacttg gacatcgaca cccaagaatt gcttgaagat ctgttgcgcg attaccaagg
    85861 cacggtcttc ctcgtctcgc acgaccgtat gttcctcgac aatgtaatta cccaaagcat
    85921 tgttttcgaa ggacaaggcc gtctgaaaga atacatcggc ggctatcagg actatatcga
    85981 cgcaaaatcg cgggaaaata aaattcagac ggcatccgca cccaaagcat ccgatgtcga
    86041 acccgccaaa gaaaaaccca aagccaaccg tacggtcaaa ctttcctaca aagaacagcg
    86101 cgaactcgac gccctgcccg acgaaatcgc cgccttggaa accgaacagg cagaaatcaa
    86161 cgcgcagctt tccgatcccg gaattttcaa agattacgaa aaagcaggag cattgcaaaa
    86221 ccgcgccgaa gaaatcgaaa tgctgctttt ggaaaagctg gaacgctggg agctgctgga
    86281 aaccaaacag aacggcaatg ccgtctgaaa tcaaacagga agaaaaaaaa tgttcaaacg
    86341 acctgaagaa atcatcgtcc tgatacttgc cgtcctctgg attgccggta cgtattttct
    86401 cgccgccctg tttggagcgg atgcttatac cgtgttaaaa atcactgccc ttaccctgct
    86461 ttggtctgcc gcatcattcc tcctttggca aaaaaaacct cagccggcat atctggccgc
    86521 tgctgctcgg ctgcctgact acctgttggt ggccgtatct gaatcaatcg gtcgcacccg
    86581 gttctttacc ttggcatgca tcatggacgt tcaaaaccat cttagccctg attcccgcaa
    86641 ccgcaggtta tctgtttaaa tggaaacaat accggcagcg caaaacaaag ccgtaagccc
    86701 gtcttttttc agacggcatc tccggtcaga ctgtcaacaa ttccgataaa atgttatact
    86761 cgcgcccgtt gccgcgccgt ccgaaaccgc cttttgggaa agcagaaatg atcagcaaac
    86821 aagaatacca agcccaagcc gcccaaggct acaaccgcat cccgctcgtg caagaactcc
    86881 ttgccgactt ggatacgccg ctttccctct atctcaaact cgccaaccgc ccctatacct
    86941 acctgctcga atccgttgtc ggcggcgaac gtttcggccg ctattccttt atcggcctgc
    87001 cttgcagcca ctatctcaaa gccggcggca aacacgtcga tgtttatcaa aacggcgaaa
    87061 tcgtcgaaca atacgacggc aatccgctac cctttatcga agctttccac aaccgtttca
    87121 aaacacccga aatcccaagc ctgccgcgct ttaccggcgg cttggtcggc tacttcggtt
    87181 acgaaaccgt ctacaatttc gaacacttcg cccaccgcct gaaaaacacc gccaaagcca
    87241 acccgctcgg cacgcccgat attttgctga tgctgtcgca agagttggcg gtaatcgaca
    87301 atttgagcgg caaaatccac ctcatcgttt atgctgatcc ctcgcagccc gacagctacg
    87361 aacgcgcccg cgaacgcctc gaagacatcc gcacccagtt gcgccaaagc tgcgccatcc
    87421 cactttccct cggcagcaaa caaacccaag cagtcagcga gttcggcgaa gaacctttca
    87481 aagcctgcgt cgataaaatc aaagactaca ttttcgcagg cgactgtatg caggtcgtcc
    87541 ccagccaacg catgagcatg gaatttaccg acaatccgct tgccctctac cgcgccctgc
    87601 gcacgctcaa cccttcgcct tacctctttt actacgattt cggcgatttc cacatcgtcg
    87661 gttcctcgcc cgaaatcctc gtccgccgcg aacgcgacga cgtcatcgtc cgccccatcg
    87721 ccggcacgcg cctgcgcggc aaaacccccg ccgaagacct tgccaacgaa caagatttgt
    87781 taagcgacgc caaagaaatc gccgaacacg ttatgcttat cgatttgggg cgcaacgatg
    87841 tcggacgcat cagcaaaaca ggcgaagtca aagtcaccga caaaatggtg attgaaaaat
    87901 actcccatgt gatgcacatc gtttccaacg tcgaaggctg tctgaaagag ggcgttacca
    87961 atatggacat cctcgccgcc accttccctg ccggcacact ctccggcgcg ccaaaagtcc
    88021 gcgcgatgga aatcatcgaa gaaatcgaac cggagaaacg cggcatctac ggcggcgccg
    88081 tcggcgtatg gggcttcaac aacgatatgg atttggcaat cgccatccgc accgccgtga
    88141 ttaaaaacaa taccctcttc atccaaagcg gcgcgggcgt ggttgccgac tccgacccaa
    88201 cttccgaatg gcaggaaact caaaacaaag cccgcgcagt ggttcgggcg gcgcagatgg
    88261 tgcaggaagg gttggataaa taaatttcag acaaccttcg cagatgaaaa gaaggtcgtc
    88321 tgaaaatcac aaaacaacat cagagatact tacataaaaa atcacaatgc aattcacatc
    88381 attagaaatt tgtgccggag caggcggaca ggctttgggc ttagaaaggg caggcttttc
    88441 ccatgttgcg ttaatcgaaa tcgaaccgtc ggcttgtcaa accctacgtt taaaccgtcc
    88501 tgattggaac gttattgaag gagatgtccg cttgtttcag ggcgaaggat atgacggtat
    88561 tgatttattg gccggcggcg tgccttgccc tcctttttcc aaagctggaa aacaactggg
    88621 aaaagatgat gaacgcgatt tattccctga ggctattcgt ctagctaagg aaactgaccc
    88681 taaagccatc atgttagaaa atgtgcgggg gttacttgac ccaaaatttg aaaattatcg
    88741 caaccatatt acagaacaat ttgccaagtt gggttacctt ggacaatgga aactacttta
    88801 tgctgccgat tacggagtat cgcaactcag acccagggtt ttatttgttg cgttgaaaaa
    88861 cgaatacacc aattttttca aatggcctga accaaattcg gaacaaccga aaaccgttgg
    88921 agaactgctt tttgacttaa tgtcagaaaa taactggcag ggggcgcata actggcgttt
    88981 gaaggccgcg cagattgcac cgaccttggt cggcggttca aaaaaacacg gcggtgctga
    89041 cttggggcct acacgatcca aacgcgcatg ggcggagttg ggtgtggatg gttcaggttt
    89101 gtgggatagt gcgccgcctg aagacttttc gggaatgcct agattgactg tccgaatgac
    89161 ggcgcgtatc caaggatttc ctgataactg gcaatttttt ggtaaaaaaa cgcctatgta
    89221 ccgccaaatc ggcaacgctt ttcccccgcc ggtagccgaa gcggtgggca ggcagattat
    89281 caaagcctta aaaaaggaaa attgaatgaa tccgcttttt acccaagaac gcaggatttt
    89341 ccataagaaa ttattggatg gcaatattct tgcaaccaac aatagaggcg tagtcagcaa
    89401 tgcggatggg agcaataccc gaagtttcaa tatagccaag gggattgccg acttactgca
    89461 ttcggaaacg gtttcggaaa gattacccgg tcagacatcg ggaaatgctt ttgaagcaat
    89521 atgcagtgag tttgtccaat ctgcttttga gaaattgcag catatcagac ccggcgactg
    89581 gaatgttaag caggtcggtt ctcgcaaccg tctggaaatc gcacgttatc agcaatacgc
    89641 acatctgaca gccttagcca aggctgccga agaaaacccc gaattggctg ccgccttggg
    89701 cagtgattac accattactc ccgatattat cgttaccaga aatttgattg cagatgcaga
    89761 aatcaaccgt aatgaatttt tagttgatga aaatattgcc acatatgcca gtttgcgtgc
    89821 gggtaatggc aatatgccgc tgctccacgc cagtatctct tgtaaatgga cgattcgcag
    89881 cgacagggct caaaatgccc gttctgaagg attgaatttg gttagaaaca gaaaaggcag
    89941 actgccgcac attgttgtcg taacggcaga accaacgcca agccgcattt catctattgc
    90001 cttgggtaca ggggaaatag attgcgttta ccattttgcc ttatatgagc tggaacaaat
    90061 tttacaatca ttgaattatg aagacgcttt ggatttgttt tacatcatgg tcaatggtaa
    90121 acgattaaaa gatatttccg accttccctt agatttggca gtttaaacag aattattcca
    90181 ctatgaacac accccccatc cttcctcccg ccatgctcgg catcctcggc ggcggacaat
    90241 taggcagaat gtttgccgtt gccgctaaaa ccatgggcta caaagtaacc gttctcgatc
    90301 ccgacccgaa tgcgccggcg gcggaatttg ccgaccgcca tttgtgcgcg ccgtttgacg
    90361 accgggccgc gttggacgaa ttggcaaaat gcgcggcggt tacgaccgaa tttgaaaacg
    90421 tcaatgccga cgcgatgcgc tctctggcaa agcataccaa cgtttccccc agcggcgact
    90481 gcgtgtccat tgcacaaaac cgcattcagg aaaaagcgcg gatacgcaaa gcaggcttgc
    90541 aaaccgcgcc gtatcaggcg gtttgcaagg ccgaagacat tactgaagca agcgcgcaat
    90601 ttttgcccgg catcctgaaa acggctacgt tgggctacga cggcaaaggt caaatccgcg
    90661 tcaaaacgtt ggacgaactc aaagccgcgt ttgccgaaca cggcggcgtg gattgcgttt
    90721 tggaaaaaat ggtggacttg cgcggcgaga tttccgtgat cgtatgccgt ctgaacgatg
    90781 aaaacgtgca aaccttcgac cccgccgaaa acatccacga aaacggcatc ttggcttatt
    90841 ccatcgtccc cgcgcggctg agtgccgacg tgcagcaaca ggcgcggcag acggcgcaac
    90901 gcttggcgga cgaattggat tatgtcggcg tattggcggt agaaatgttt gttgtcggcg
    90961 acacacatga attgctcgtc aatgaaaccg ccccgcgcac gcacaattcc ggccaccata
    91021 cgatagatgc ctgcgccgca gaccagttcc aacagcaggt acgcattatg tgcaacctgc
    91081 cgcccgccga caccaaatta ttatcccctt gctgtatggc gaatattttg ggcgacgttt
    91141 ggcaggaaga tggcggcgaa ccggattggc tgccgttgca aagccggccg aatgcacacc
    91201 tgcacctata cggaaaaaaa accgcacaga aaggtcggaa aatgggacac tttaccgttt
    91261 tgaccaccga ttcggacacc gcatttcaag aagcaaaaaa actgcatcag tccctataat
    91321 ccgaccaacg gcacagatgc cgtctgaagc cgccgacata ccgccatgag cctgctgaca
    91381 ctgttacgcc ccgccaccgt gcaagactgc aaagacatct tcaaagtcca cctgcactcc
    91441 gtccaataca cctgcatcct cagctacaac gaacacgccc taaaagtatg ggaaggcttg
    91501 ttaaacacag aaagctacct gccgaccatt tccgatcccg acaaagcact ttgggttgcc
    91561 gaatacaaag gcaacataca gggatttttc caaatcgact gccaagaagc acaactcgat
    91621 gcgctttacg tccaccccct gttccacaat ctcggactgg gcactgccct gctccgtcag
    91681 gcagaaacca tcgcccacaa atccggtttg agctttctga aactctacgc atctctcaac
    91741 tccgtcccgt tttaccttct caaccgctac gaatcgctcg gttcggcagt attgcagctt
    91801 gaccccagca tcaaaatcaa atgcgaactg atgcgcaaac acctctaatc ccgccttata
    91861 accaaaactg ataatcttca aatcaaacat tatttccaca agcgcagaca gccgtataaa
    91921 gttgccccat ctaccaaccg acaggacaag catatgaaaa cctacgcaca ggcactctat
    91981 accgcagccc tgctcaccgc ctgcagcccc gcagccgatt caaaccatcc gtccggacaa
    92041 aatgccccgg ccaataccga atccgacgga aaaaacatta ccctgctcaa tgcctcatac
    92101 gatgtgacac ggtattttta caaagaatac aaccacttat ttatcaaaac ataccaatcc
    92161 gaacaccccg gcacatccgt cagcatccaa caatcccacg gcggcttcag caaacaggca
    92221 ttatccgtag ccaacggcct tcaagccgat gtcgtaacca tgaaccaatc ttccgacatc
    92281 gacctgctcg aaaaaaagga ctggtagaaa aaggctggca acaagccctc cccgatcacg
    92341 ccgcacccta caccagcact atggttttcc ttgtccgaaa aaacaacccc aaacagatcc
    92401 gcgattggaa cgaccttgcc aaagacggcg ctaacatcgt catcgccaag acctcgggca
    92461 acggacgcta cgccttcctc ggcgcatacg gttacggtct gaaagccaac aacggcaacg
    92521 agcaggaagc ccaaaaactc gtcgcatcca tcctcaaaaa cacacccgtt tttgaaaacg
    92581 gcggacgcgc cgccgccacc accttcacac aacgcaacat cggcgacgta ctcatcactt
    92641 ttgaaaacga agccaactac gtcagcaaaa aactgaccca aggacagttt gaaatcgtct
    92701 atccgagcta caccatttcc gccgaaagcc ccgttgccgt cgtcaacagc gtcgtcgcga
    92761 aaaaaggcac gcaaaaaacc gcccgcgcct atctcgaata cctttggagc gaaccggcgc
    92821 aagaactcgc cgcctcactc tacctgcgtc cccgcaaccc cgaagtattg gcacgacaca
    92881 aagccgactt ccccgactta gacaccttcc ctcccgaaga aaaattcggc ggatgggaca
    92941 acatcatgaa aacctacttc gccgacggcg gcgtattcga ccgcctgacc gcacaaaaat
    93001 gaccattatg ccgtctgaac cgcctttcag acggcatttc acacatcaaa acagaaaacc
    93061 gcacccaaaa tcatcatcac aagacacaac ggcaaaccgg atacaaaacc caacaccgcc
    93121 gccaaaatgc cgtctgaagc cgtcggcgca accatcctta tctggggcgc gcctcatcaa
    93181 aggggcgcat aactttcctt aaccatccct tttgacagcc aaataaacag tgcttttgcc
    93241 tgtcagaaac aaaaccaata ttttgcggtt cgtatcggaa catttataaa acatcagatt
    93301 tccgtgtttc gcgtttccgg caattcaaga ttagcaaaac cattaacctg cttcgcaagg
    93361 caggttttta aaactttatc aggcgttcga gcatatcggt tcgaccgacc cgcatttccg
    93421 gcaatcaaca ctgccgacgg ttgccgcatt tctttttgat atacaatcac actttcaaaa
    93481 aacatacgac ggcaacaaaa tcatcctccc cccgcttggc aaacatccgg caaattaaaa
    93541 ccccgcccga aacagccatc ggacaaataa caggaaaaaa tatgaatacc gacttccaaa
    93601 agatcctcaa acaactgggc aaacaggcca aaaaagaagc ggcagaaaaa caggaagccg
    93661 aaaaaaacaa gcaaaaacaa gaatatgatt tggatttttc acaagcagtc ggacaagtct
    93721 ccccattaaa aaaccggcag caatattatg cgcagtccga caaaaccccc atcaaagccc
    93781 gtcccaaaga caaccgcacc gacgaagaaa actacttcta cataggcagc acatacaacg
    93841 acccacccgc cagcttctgc aaaaacggac agggaaaaaa cgacatccaa cgcctgaaaa
    93901 acggatacta tcccgtcgtt accgatgtcg acctgcacgg ctacacccaa gaagaagcac
    93961 aaaaagtcct caacgaattc atcgcattca cccaaaaacg cggcgtatgc ggcgaaatca
    94021 tccacggcag cggattaggt tccaaaggct acaaacccgt tctgaaaaac atgacccgaa
    94081 actggctgat gcaacacccc gacgtactcg cctacgtcga accccgggaa ggcaacgacg
    94141 gctgcgtccg catcctgctc aaacgcaaat taaggcagca agactgacaa atttctccga
    94201 cagacttggc agcaccgcac aatatccgta taatttgcaa accatttctg ggggcgacct
    94261 tggtttcgac gggggttgcg aagcagatgc gggcataccg gggtctcaga ttcccgtaaa
    94321 acactgaatt caaatagtcg caaacgacga aacttacgct ttagccgctt aaggctagcc
    94381 gttgcagcag tcggtcaatg ggctgtgtgg cgaaagccac cgcaacgtca tcttacattg
    94441 actggtttcc agccgggtta cttggcagga aataagactt aaggtaactg gtttccaaaa
    94501 ggcctgttgg tcggcatgat ggaaataaga ttttcaaata gacacaacta agtatgtaga
    94561 acgctttgta gaggactttc ggacgggggt tcgattcccc ccgcctccac catgccctat
    94621 tttcagcagt atcctgcaac acaaaaaatc cttgaacttc aaaagtttca aggctttttt
    94681 attgccttga atatgatggc gtatgcttga gttttcgcaa tttatggggt aacttttggg
    94741 gtaacttttc aggaatcacc ctaaaaaata tcttaaaaat acaaaagcca tcacgcattt
    94801 tttaaaaagg ttacccaaaa cttaaatgaa gcctttattt ataaggcttg cagctttcca
    94861 aacaagttac ccccaaacgg ataaaaaatg ccgaag
//
LOCUS       DBJGGI010000002        67497 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.002, whole genome shotgun sequence.
ACCESSION   DBJGGI010000002 DBJGGI010000000
VERSION     DBJGGI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 67497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67497
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.002"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(34..108)
                     /locus_tag="ACSIGJ_000098"
     tRNA            complement(34..108)
                     /locus_tag="ACSIGJ_000098"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(73..75),aa:Glu,seq:ttc)
     gene            complement(126..202)
                     /locus_tag="ACSIGJ_000099"
     tRNA            complement(126..202)
                     /locus_tag="ACSIGJ_000099"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(166..168),aa:Arg,seq:acg)
     gene            complement(313..1986)
                     /gene="recN"
                     /locus_tag="ACSIGJ_000100"
     CDS             complement(313..1986)
                     /gene="recN"
                     /locus_tag="ACSIGJ_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HHQ1329099.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPALKAELREQGLLDEGGEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGGQLIDIHGQNDHHSLNQEAAQRELLDAFVGGRVQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGHSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAGRLSSETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(2170..2772)
                     /gene="scpB"
                     /locus_tag="ACSIGJ_000101"
     CDS             complement(2170..2772)
                     /gene="scpB"
                     /locus_tag="ACSIGJ_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HHQ1329100.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLIEMPPTDEEEPETVPSDTLPN"
     gene            complement(2873..3814)
                     /gene="ylqF"
                     /locus_tag="ACSIGJ_000102"
     CDS             complement(2873..3814)
                     /gene="ylqF"
                     /locus_tag="ACSIGJ_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HHQ1329101.1"
                     /translation="MAIQWFPGHMNKARKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDDNSWLEWIAKKRGAVLSGGRVNYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3895..4749)
                     /gene="rapZ"
                     /locus_tag="ACSIGJ_000103"
     CDS             complement(3895..4749)
                     /gene="rapZ"
                     /locus_tag="ACSIGJ_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HHQ1329102.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDLGYFCVDNLPLEMLPSLVSYH
                     IERADETELAVSVDVRSGIDIAQAREQIAYLRGLGHRVEVLFVEAEEAVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVVLESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLAQEM
                     VDGIERFVTRWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSGR"
     gene            complement(4730..5692)
                     /gene="hprK"
                     /locus_tag="ACSIGJ_000104"
     CDS             complement(4730..5692)
                     /gene="hprK"
                     /locus_tag="ACSIGJ_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HHQ1329103.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANDLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            complement(5696..6145)
                     /gene="ptsN"
                     /locus_tag="ACSIGJ_000105"
     CDS             complement(5696..6145)
                     /gene="ptsN"
                     /locus_tag="ACSIGJ_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HHQ1329104.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHASVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVASAEEARAILTEE"
     gene            complement(6330..7220)
                     /gene="ubiA"
                     /locus_tag="ACSIGJ_000106"
     CDS             complement(6330..7220)
                     /gene="ubiA"
                     /locus_tag="ACSIGJ_000106"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HHQ1329105.1"
                     /translation="MNLKSPLFLRLSDRLDVYIRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQFYLGLAFSFGIP
                     MAFAAVGNSVPVEAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFAAIFAHTFFAK"
     gene            complement(7210..7617)
                     /locus_tag="ACSIGJ_000107"
     CDS             complement(7210..7617)
                     /locus_tag="ACSIGJ_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329106.1"
                     /translation="MNIIEIISRNRFLKQIYPSGIMDISLVSFSTDLSNCILTIRTST
                     KPSVEIEKWGLWLKDYDTVEIELRNSFIKGMKCQNWSHNNRNICQVEIKNQEDGLKII
                     RFYDNNSNWLLELEVYGLVFQGCKTYMKEGYES"
     gene            complement(7626..7721)
                     /locus_tag="ACSIGJ_000108"
     CDS             complement(7626..7721)
                     /locus_tag="ACSIGJ_000108"
                     /inference="COORDINATES: protein motif:HMM:NF026993.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH/endonuclease VII fold putative polymorphic
                     toxin"
                     /protein_id="HHQ1329107.1"
                     /translation="MRQPASKPHFNIRDSNLDTGHVDGTHGHYNF"
     gene            complement(7741..8217)
                     /locus_tag="ACSIGJ_000109"
     CDS             complement(7741..8217)
                     /locus_tag="ACSIGJ_000109"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate--pyruvate lyase family protein"
                     /protein_id="HHQ1329108.1"
                     /translation="MEHLFGKWLPDLPAPVSDGIDLPMSRLLKARSLTAALCALPHTF
                     SVELLKLGEAETEYGRRRVRDVLLKLDGTAVVQARSACSVGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSSEGCGRYFAARRSRFSHQGEEMLLTEYFLPELKRFIG"
     gene            complement(8232..8879)
                     /gene="bioD"
                     /locus_tag="ACSIGJ_000110"
     CDS             complement(8232..8879)
                     /gene="bioD"
                     /locus_tag="ACSIGJ_000110"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HHQ1329109.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QDIAEDIAVHRKIMGIPMQEADEQRLTMPEIFSHPASPHLAARLDGRGLDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTEKLLTIDHIQQQAYPVILVTSGRLGSINHTLLSFV
                     VLKQYGIRLHSLVFNHIHDSRDAHVAQDSLNYLQCRLKADFPEAEWMELAKTGAV"
     gene            complement(8876..10177)
                     /gene="bioA"
                     /locus_tag="ACSIGJ_000111"
     CDS             complement(8876..10177)
                     /gene="bioA"
                     /locus_tag="ACSIGJ_000111"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HHQ1329110.1"
                     /translation="MPSEHQHTSSLLNFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVENQMKQMAHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKNRFDDEWDGADLQPVRALFEAHHVDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGILTKLADKTVQILKEHSK"
     gene            complement(10395..10619)
                     /locus_tag="ACSIGJ_000112"
     CDS             complement(10395..10619)
                     /locus_tag="ACSIGJ_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329111.1"
                     /translation="MSVWDIWDRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEEPCIG
                     RCYAPMPESPDFANAVAMAVAMICIVFIYD"
     gene            complement(10811..11296)
                     /locus_tag="ACSIGJ_000113"
     CDS             complement(10811..11296)
                     /locus_tag="ACSIGJ_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HHQ1329112.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAVKSSGKVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFFHDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
     gene            complement(11472..11780)
                     /locus_tag="ACSIGJ_000114"
     CDS             complement(11472..11780)
                     /locus_tag="ACSIGJ_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329113.1"
                     /translation="MTNNVDLTIPAKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLKSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLEDLDKELC
                     "
     gene            complement(11764..12066)
                     /locus_tag="ACSIGJ_000115"
     CDS             complement(11764..12066)
                     /locus_tag="ACSIGJ_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HHQ1329114.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKGMVEHYYDKQR"
     gene            complement(12140..14503)
                     /gene="pheT"
                     /locus_tag="ACSIGJ_000116"
     CDS             complement(12140..14503)
                     /gene="pheT"
                     /locus_tag="ACSIGJ_000116"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HHQ1329115.1"
                     /translation="MQFSYSWLKTQANPDLSADKLEHLLTMAGLEVEEIDTAAPAFSG
                     VVVAEVKSVEKHPDADRLNVTQVDAGTGELVQIVCGAPNVKPGIKVPCSLPVAVLPGN
                     FKIKPTKMRGVPSNGMLCSTNELGLPDDGVDGLHILPEDAPVGTNIREYLDLDDMLFT
                     LKITPNRADCLSVKGIAREVSALTQCAFTPVEIQTAPIGSEKKQAVRIDAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LIVRRAQNGETLACLNEKTVTLADNTLVVADEKGALSLAGLMGGAASAVSDGTQNIVL
                     EAAWFEPEIIVGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGKLPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRITAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMSELPETRRPRFAVYNEMAARGYRE
                     VISYAFVDEQWELDFAANAAPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIACVFGKGSDGRFVQNERIGGLWYGAAMPEQWGEKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEVMNHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGVATEAGARLRS"
     gene            complement(14594..15343)
                     /locus_tag="ACSIGJ_000117"
     CDS             complement(14594..15343)
                     /locus_tag="ACSIGJ_000117"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HHQ1329116.1"
                     /translation="MKKWTRDETLVALYLYYIIPFQKVSKDNPVIQEYAEILGRTPSA
                     LGMKIGNLGRLDPTLKVKNISGLSNGSKMDVVVWNEFSGDWEQLNKEFEGVISQYQSN
                     DENSNIEIESPEIPKGRERFARISVRVNQGFFRSSVLAAYNNQCCITGLKQPELLVAS
                     HIKPWGEDKDNRLNPRNGLCLNALHDKAFDRGLLGIDENFKIIFSPLLAKTEGFDDLF
                     KPYENRMIRLPERLNPSLEFLKFHRENIFQS"
     gene            complement(15349..15639)
                     /locus_tag="ACSIGJ_000118"
     CDS             complement(15349..15639)
                     /locus_tag="ACSIGJ_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329117.1"
                     /translation="MCCVGLPYQNKDFRNMRLSDYILNNKININNLSDKQVQEIIDYF
                     SSEIDDWLLASEKELKDYDVYLYKLVLDGQQYVQGIRKSVLYDFLMENQKDS"
     gene            complement(15632..15841)
                     /locus_tag="ACSIGJ_000119"
                     /pseudo
     CDS             complement(15632..15841)
                     /locus_tag="ACSIGJ_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            complement(15948..16361)
                     /locus_tag="ACSIGJ_000120"
     CDS             complement(15948..16361)
                     /locus_tag="ACSIGJ_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HHQ1329118.1"
                     /translation="MDKLTPEQRKKCMQSNKSTGTKPELVLAKAMWALGLRYRKNSGS
                     IFGKPDFSFKKYKVAVFVDGEFWHGKDWEQKKAVIKGNREFWIAKIERNIQRDIEVTG
                     RLKAEGWTVLRFWSNDVVKNTTCCAEKVKEIIQTR"
     gene            complement(16378..17367)
                     /gene="pheS"
                     /locus_tag="ACSIGJ_000121"
     CDS             complement(16378..17367)
                     /gene="pheS"
                     /locus_tag="ACSIGJ_000121"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HHQ1329119.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFE"
     gene            complement(17608..17967)
                     /gene="rplT"
                     /locus_tag="ACSIGJ_000122"
     CDS             complement(17608..17967)
                     /gene="rplT"
                     /locus_tag="ACSIGJ_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HHQ1329120.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(17980..18177)
                     /gene="rpmI"
                     /locus_tag="ACSIGJ_000123"
     CDS             complement(17980..18177)
                     /gene="rpmI"
                     /locus_tag="ACSIGJ_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HHQ1329121.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(18324..18845)
                     /gene="infC"
                     /locus_tag="ACSIGJ_000124"
     CDS             complement(18324..18845)
                     /gene="infC"
                     /locus_tag="ACSIGJ_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HHQ1329122.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(18863..20776)
                     /gene="thrS"
                     /locus_tag="ACSIGJ_000125"
     CDS             complement(18863..20776)
                     /gene="thrS"
                     /locus_tag="ACSIGJ_000125"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HHQ1329123.1"
                     /translation="MLNITLPDCSVRQYESPVTVAQIAASIGAGLAKAAVAGKVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     EDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKDYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWRTI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVRLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFVGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVIGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(20829..20905)
                     /locus_tag="ACSIGJ_000126"
     tRNA            complement(20829..20905)
                     /locus_tag="ACSIGJ_000126"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20869..20871),aa:Val,seq:gac)
     gene            21084..22199
                     /gene="tgt"
                     /locus_tag="ACSIGJ_000127"
     CDS             21084..22199
                     /gene="tgt"
                     /locus_tag="ACSIGJ_000127"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HHQ1329124.1"
                     /translation="MLKFTLHKKDGLARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGETTREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEEFDFPGLAVGGLSV
                     GEPKPEMYRMLHAVGPMLPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKPDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMRDAVEQGKFADWQAQFHENRARGVD"
     gene            22209..23291
                     /gene="hemH"
                     /locus_tag="ACSIGJ_000128"
     CDS             22209..23291
                     /gene="hemH"
                     /locus_tag="ACSIGJ_000128"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HHQ1329125.1"
                     /translation="MPSEAASDSILPLYPTCTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRIVELPKWLWYPILHGLVLTFRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSIADVLAELKSQGVGRLLA
                     IPLYPQYTASSSGAAVDKVCEQLLLQRNQMSVRTISRFYDDAGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDEYTVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALTGREQFYEAGGKNYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            23482..23949
                     /locus_tag="ACSIGJ_000129"
     CDS             23482..23949
                     /locus_tag="ACSIGJ_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c2"
                     /protein_id="HHQ1329126.1"
                     /translation="MNTTRLPTAFILCCLCAAASAADNSIMTKGQKVYESNCIACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKNQTDKMPSETGNPASDGIQTKPF"
     gene            complement(23946..25667)
                     /locus_tag="ACSIGJ_000130"
     CDS             complement(23946..25667)
                     /locus_tag="ACSIGJ_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HHQ1329127.1"
                     /translation="MNSLFLLGGALLFLSVVSTTLSARLGMPLLLVFLAVGMLAGEEG
                     VGGIAFNNVVMANFISQLALAVILLDGGLRTQLSSFRIALKPASVLASWGVFATVLPL
                     GLFATFYLGLDWKFGVLMAAIVGSTDAGAVFSLLRNSGVRLNERVQATLEIESGANDP
                     MAVFLVTALITMIMQPAESGAAAFVRMLALQIGFGLLTGWAGGKILAKLVRRLNLAEG
                     LYALMIVSGGLLVFAFTNTIGGSGFLAVYLAGIIVGNQRNRATEHVLRVMDGLAWLAQ
                     ATLFVMLGLLVSPAGVLDRAAEALAIAAFLMLVARPLAVFGGLWKFNYSLREKAYISW
                     LGLRGAVPISLAMMPLVMGVPNSHLLFDVAFAVVVLSLLIQGTTIPVMARLLKVAMPN
                     KPEPKDTHDIWLAEKEIVRMSAFKVVAESEAEGHHPDTVEPISDSFDARCFALIRNGS
                     RIEMQSDTVLQAEDLAWYILPDGKVDKMAKYFTETGIGVRENFDFFGEFVVSPAARSG
                     DLALAYGLKLEAGEEGLSLAELFDKRSDSQEPVEGDRIDIGGFMLTAKEVDGDGNIGS
                     MGLKVPR"
     gene            25871..26611
                     /locus_tag="ACSIGJ_000131"
                     /pseudo
     CDS             25871..26611
                     /locus_tag="ACSIGJ_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            26680..28218
                     /gene="lnt"
                     /locus_tag="ACSIGJ_000132"
     CDS             26680..28218
                     /gene="lnt"
                     /locus_tag="ACSIGJ_000132"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HHQ1329128.1"
                     /translation="MFSKLDKYWQHPALYWPLLILFAAATPFAFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAASSAYLFGLTAYTAQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPQGIKTGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLAIDNTARSGKRLLPAILIAALLAAGYTAQQT
                     DFTRPDGSRSTVALLQGNIAQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPI
                     MRQNLPENILAQFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPLLTTPLYKMMDMPLSDFRKGGGKQSAMMMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANVSNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYIGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            28482..29345
                     /gene="rpoH"
                     /locus_tag="ACSIGJ_000133"
     CDS             28482..29345
                     /gene="rpoH"
                     /locus_tag="ACSIGJ_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HHQ1329129.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNVLSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(29502..29978)
                     /locus_tag="ACSIGJ_000134"
     CDS             complement(29502..29978)
                     /locus_tag="ACSIGJ_000134"
                     /inference="COORDINATES: protein motif:HMM:TIGR00645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HHQ1329130.1"
                     /translation="MESRAFVAARKGNVSSKISKTRTGYGFFAKMIFASRWLQVPIYA
                     GLTVVRAICAYKFLKSLKHLVMNLDVSDENAIMLAVLNLIDVVMIANLLTMVQIGGYE
                     SFVSRLRIDDHPDRPEWLSHVNAPVLKVRLSMSIIGIHPSICSKHLSIPPICRKSS"
     gene            30078..30962
                     /locus_tag="ACSIGJ_000135"
     CDS             30078..30962
                     /locus_tag="ACSIGJ_000135"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HHQ1329131.1"
                     /translation="MSSGNIRIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRHLENGIREQISSHLARGKVECKIQIQDAENGNQSLELNRVLVGQLAEINKNLR
                     KHYDLAKLGVADILRFPGVLASQKENTEALAKTIVELADKALKDFTAARRREGKKLGE
                     HLLQRIKNMEEIIDALSEIFPALVEAHKEKIRTRLAEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(31053..31568)
                     /locus_tag="ACSIGJ_000136"
     CDS             complement(31053..31568)
                     /locus_tag="ACSIGJ_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HHQ1329132.1"
                     /translation="MMILKTAPLKRRFAAILYEMLLVGAATCLAALLAGIAAIFLNPV
                     SIAVSALVTSILIMGAWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALVWLILPWGFALLNPDRQFLYDFLA
                     GTRLVDVKEQS"
     gene            complement(31644..32063)
                     /locus_tag="ACSIGJ_000137"
     CDS             complement(31644..32063)
                     /locus_tag="ACSIGJ_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329133.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(32184..33182)
                     /gene="holA"
                     /locus_tag="ACSIGJ_000138"
     CDS             complement(32184..33182)
                     /gene="holA"
                     /locus_tag="ACSIGJ_000138"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HHQ1329134.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEAVDALRAAAKKQGY
                     LNREAYTADASFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKNGGEALQDFAARLP
                     EDTVTLVLLPKLEKTRLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHAVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVPRVCRLLDGLEEEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKQLVVSLAE"
     gene            complement(33184..33663)
                     /gene="lptE"
                     /locus_tag="ACSIGJ_000139"
     CDS             complement(33184..33663)
                     /gene="lptE"
                     /locus_tag="ACSIGJ_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HHQ1329135.1"
                     /translation="MNKIFLTAAALMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRILDYADNEILGKQEEEETLWAEMRQDVAEQIVRRLTFLKAE"
     gene            complement(33715..34494)
                     /gene="pgeF"
                     /locus_tag="ACSIGJ_000140"
     CDS             complement(33715..34494)
                     /gene="pgeF"
                     /locus_tag="ACSIGJ_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HHQ1329136.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDDPETVRRNREIVQQQVGLPVAYLNQIHSTIVVNAAEALDGTPDADAS
                     VDDTGKAVCAVMTADCLPVLFCDRAGTAVAAAHAGWSGLAGGVLQNTIAAMKVPPVEI
                     MAYLGPAISADAFEVGQDVFDAFCTSMPEAAAAFEPIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDIFFSYRRDGATGRMASLIWLDGNAV"
     gene            34647..34871
                     /locus_tag="ACSIGJ_000141"
     CDS             34647..34871
                     /locus_tag="ACSIGJ_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329137.1"
                     /translation="MMPLYTFSELYMLQQGTAHQAPHCVLPERGCPPIRFYRYKQTGF
                     NRKGMGIKSISDTSRAMPSENQSPLSDGIV"
     gene            34948..35895
                     /locus_tag="ACSIGJ_000142"
                     /pseudo
     CDS             34948..35895
                     /locus_tag="ACSIGJ_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            complement(36048..37172)
                     /gene="rluD"
                     /locus_tag="ACSIGJ_000143"
     CDS             complement(36048..37172)
                     /gene="rluD"
                     /locus_tag="ACSIGJ_000143"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HHQ1329138.1"
                     /translation="MQNTSFDNESDYSDDSDFASASGTENRIGLTVPPELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MDLDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQIPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAAVKFGGKPAVTHVKVLERYLAHSYIECSLGTGRTHQIRVHMREANHPLAGDPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            37171..37974
                     /locus_tag="ACSIGJ_000144"
     CDS             37171..37974
                     /locus_tag="ACSIGJ_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HHQ1329139.1"
                     /translation="MKKILLTVSLGLALSACATQGTADKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAKKIIGSYQNTRYVEESL
                     AILELAYKKLDKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(38044..40263)
                     /locus_tag="ACSIGJ_000145"
     CDS             complement(38044..40263)
                     /locus_tag="ACSIGJ_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HHQ1329140.1"
                     /translation="MFRRWFLPCWVVGVAVSFALSVVPHWPFWLAAFAVLAVLARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVTDMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGVGGV
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWRQADADGGLSDGIGLMRALSVGEQSALRP
                     GLWQAFRPLGLTHLVSISGLHVTMVAVLFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPLAVLGVG
                     TWLSFGLVAALIWACAGRLYEGKRQTAVRGQWAASVLSLVLLGYLFASLPLVSPLVNA
                     VSIPWFSWVLTPLALLGSVVPFAPLQQAGAFLAEYTLRFLVWLADVSPEFAVAAAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVSYRPEAVPENEAAVTVWDAGQGLSVL
                     VRTANRHLLFDTGTVAAAQTGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLRPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRVYKFYWQKKPFE"
     gene            40295..40570
                     /locus_tag="ACSIGJ_000146"
     CDS             40295..40570
                     /locus_tag="ACSIGJ_000146"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329141.1"
                     /translation="MIKQSHKTVITRFENDKNANEKPQYYPRQRQTFHRHSRKSGNPK
                     TKSRRNLSERTEPQKPASHLRLNNRTKKAEIKTSGFFFKLAIKHQNE"
     gene            40649..45430
                     /locus_tag="ACSIGJ_000147"
     CDS             40649..45430
                     /locus_tag="ACSIGJ_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HHQ1329142.1"
                     /translation="MKAKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNKRIATVVDPQYAVSVKHA
                     KAEVHTFYYGQYNGHNDVADKENEYRVVEQNNYEPHKAWSASNLGRLEDYNMARFNKF
                     VTEVAPIAPTDAGGGLDTYKDKNRFSSFVRIGAGRQLVYEKGVYHQEGNEKGYDLRDL
                     SQAYRYAIAGTPYKDINIDQTMNTEGLIGFGNHNKQYSAEELKQALSQDALTNYGVLG
                     DSGSPLFAFDKQKNQWVFLGTYDYWAGYGKKSWQEWNIYKKEFADKIKQHDNAGTVKG
                     NGEHHWKTTGTNSHIGSTAVRLANNEGDANNGQNVTFEDNGTLVLDQNINQGAGGLFF
                     KGDYTVKGANNDITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQG
                     QLKVGDGTVILNQKADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRL
                     DANGNDLTFEHIRNVDEGARIVNHNTDHASTITLTGKSLITNPNSLSVHSIQNDYDED
                     DYSYYYRPRRPIPQGKDLYYKNYRYYALKSGGNVNAPMPENGVAENNDWVFMGYTQEE
                     AKKNAMNHKNNQRISGFSGFFGEENEKGHNGALNLNFNGKSAQNRFLLTGGANLNGKI
                     SVTQGNVLLSGRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKAAEIAVNQ
                     SASFSSGRNVSDITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALN
                     SFDATRINGNVNLSQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLS
                     LADSHIHLNNASDAQSANKYHTIKINHLSGNGHFHYLTDLAKNLGDKVLVKESASGHY
                     QLHVQNKTGEPNQEGLDLFDASSVQDRSRLFVSLANHYVDLGALRYTIKTENGITRLY
                     NPYAGNRRPVKPAPSPAANTASQAQKATQTDGAQIAKPQDIVVAPPSPQANQAEEAKR
                     QQAKAEQVKRQQAEAGRKSAELSAKQRAGEEERRQTAQSQPQRRKRRAAPQDYMAVSQ
                     DRPKRRRHRSVQQNNVEIAQAQAELARRQQEERKAAELLAKQRAEAEREAQALAARRK
                     AEAEEAKHQAAELAHRQEAKRKAAESAKRKAEEEEHRQTAQSQPQRRKRRAAPQDYMA
                     VSQDRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDALEDEDVS
                     ESVDTSDKQPQDNTELHEKVEAVSLQPRAAQPRTQAAAQADAVSTNTNSALSDAMAST
                     QSILLDTGASLTRHIAQKSRADAEKNSVWMSNTGYGRDYASAQYRRFSSKRTQTQIGI
                     DRSLSENMQIGGVLTYSDSQHTFDQAGGKNTFVQANLYGKYYLNDAWYVAGDIGAGSL
                     RSRLQTQQKANFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGDDSVK
                     VSSMAVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGKSFAYKADNQQ
                     QYSAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
     gene            complement(45506..46708)
                     /gene="trpB"
                     /locus_tag="ACSIGJ_000148"
     CDS             complement(45506..46708)
                     /gene="trpB"
                     /locus_tag="ACSIGJ_000148"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HHQ1329143.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALKELEQAYNEAKNDPE
                     FWAEFRRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     ARRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VIGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFYPYIEEENVRLVGVEAGGLGVDTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIEL"
     gene            complement(46791..47351)
                     /locus_tag="ACSIGJ_000149"
     CDS             complement(46791..47351)
                     /locus_tag="ACSIGJ_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HHQ1329144.1"
                     /translation="MMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFRMP
                     SHGEAAHSEKAFDAEKIKAFLHERGIASCPTVLKAAREHGNASDKFLKVVETVDLAAV
                     LAKIPDCRHICTTGGKATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRA
                     YPLSLAKKAAAYRAFFEMAGLCEKQL"
     gene            47696..48475
                     /gene="rsmA"
                     /locus_tag="ACSIGJ_000150"
     CDS             47696..48475
                     /gene="rsmA"
                     /locus_tag="ACSIGJ_000150"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HHQ1329145.1"
                     /translation="MKEHKARKRFGQNFLQDTRIIGDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGISGKKKIVG
                     NLPYNISTPLLFKLAEVADDVADMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     ELLIDVPPESFDPAPKIDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFRQRRKTIRNN
                     LKELADDDDLQAVGISPQDRAEHIAPEKYVALSNYLADKAV"
     gene            48490..49059
                     /locus_tag="ACSIGJ_000151"
     CDS             48490..49059
                     /locus_tag="ACSIGJ_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HHQ1329146.1"
                     /translation="MESNLQKTERLIREINRLHAQYSQDYFETGKVRKINLSHTLKNV
                     PTEHILSYRLNLHEAVNDYLAFADTRGIDFFYRVKTAESIHDKINRYLARGTQYPTNN
                     ILNDIFGARLIWPSETVAGILEKLDGWKTEYGLKNWYLRDAGGYIGIHIYFKNSSNFY
                     YPWELQVWDEKDAKANIENHMAYKRNFVR"
     gene            49170..49625
                     /locus_tag="ACSIGJ_000152"
     CDS             49170..49625
                     /locus_tag="ACSIGJ_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329147.1"
                     /translation="MKYTAALLTFLLTACMNPNDAFFQNRRYQMPEAQLNGSNAVFHY
                     GYSQNPDHDLLVDQVRVPDAYRASFARDWIAKEQGYRKNDCVLEKAESGGVTYYTCDA
                     EKGFNGTRFVIYVVNMKNDKGYVKVYRGMNKPNQEELTKLVADLNKFYP"
     gene            49667..50395
                     /locus_tag="ACSIGJ_000153"
     CDS             49667..50395
                     /locus_tag="ACSIGJ_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HHQ1329148.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLDNITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            50468..50827
                     /locus_tag="ACSIGJ_000154"
     CDS             50468..50827
                     /locus_tag="ACSIGJ_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329149.1"
                     /translation="MYFVDRTAAVLKPTARFLEWLKSTDENMPDLTIEQLRADCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEADLNITPVFDNMM"
     gene            50832..51272
                     /locus_tag="ACSIGJ_000155"
     CDS             50832..51272
                     /locus_tag="ACSIGJ_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HHQ1329150.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASIAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGRAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGKTVRQCIMPDMTDKESYRRLKVWVLWRQPKKTAETDTSD"
     gene            51303..52577
                     /gene="folC"
                     /locus_tag="ACSIGJ_000156"
     CDS             51303..52577
                     /gene="folC"
                     /locus_tag="ACSIGJ_000156"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HHQ1329151.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVSEVKKRMNLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGFKTGTLTSPHLLHYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNALAAVDIFIREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPASLVAHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDGPARNRNALPFPALRGAYQLSNAACALTVLECLDDRLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRNLINLAYAQKRTAV
                     FSMLSDKDIDGVSETVKDQFDEWYIAPLDVPRGMTADALKAKLEQHHIENIQTFAAVR
                     DAYRAAASKAGEDDRIVVFGSFHTVADVMSVL"
     gene            52591..53631
                     /gene="ftsN"
                     /locus_tag="ACSIGJ_000157"
     CDS             52591..53631
                     /gene="ftsN"
                     /locus_tag="ACSIGJ_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HHQ1329152.1"
                     /translation="MSENKQNEVLTGYEQLKRRNRRRLVTASSLVAASCILLAAALSS
                     DPADSNPAPQAGETGATESQTANTAQTPALKSAAENGETAADKPQDLAGEDKPSAADS
                     EISEPENVGAPLVLINDRLEDSNIKGLEESEKLQQAETAKTEPKQAKQRAAEKVSATA
                     DSTDTVAVEKPKRTAEPKPQKAERTAEAKPKAKETKTAEKVADKPKTAAEKTKPDTAK
                     SDSAVKEAKKADKAEGKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKAGKKAA
                     IQAGYAEKERALSLQRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKL
                     RVHGIAGQVTNE"
     gene            53624..54121
                     /locus_tag="ACSIGJ_000158"
     CDS             53624..54121
                     /locus_tag="ACSIGJ_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HHQ1329153.1"
                     /translation="MNSLPIADLLASAVIAACIVISTMRGVIAEAGSMVAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLIMLASKTDLPDTEEWQQSYTVPFFVSLSEAVLNHTDNAPE
                     SLDDD"
     gene            54315..55859
                     /gene="purF"
                     /locus_tag="ACSIGJ_000159"
     CDS             54315..55859
                     /gene="purF"
                     /locus_tag="ACSIGJ_000159"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HHQ1329154.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVSEVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHINTGSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLALGSQTDSEGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFNGTIIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLNKPYREGLIKNR
                     YIGRTFIMPGQSTRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            55959..56450
                     /gene="greB"
                     /locus_tag="ACSIGJ_000160"
     CDS             55959..56450
                     /gene="greB"
                     /locus_tag="ACSIGJ_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HHQ1329155.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGVDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            56509..57135
                     /locus_tag="ACSIGJ_000161"
     CDS             56509..57135
                     /locus_tag="ACSIGJ_000161"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HHQ1329156.1"
                     /translation="MRKIRTKICGITTPEDALYAAHAGADALGLVFYPQSPRAIDIIK
                     AQKIAAALPPFVSVVALFINESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIK
                     AIRVQTASDIRNAATRFPNAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
     gene            complement(57609..60440)
                     /gene="lbpA"
                     /locus_tag="ACSIGJ_000162"
                     /gene_synonym="tbpA"
     CDS             complement(57609..60440)
                     /gene="lbpA"
                     /locus_tag="ACSIGJ_000162"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HHQ1329157.1"
                     /translation="MNKKHGFPLTLTALAIATAFPAYAAQAGAAALDAAQSQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSRGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVEYGIGRLDAFRQTYDIKRKTTEPFFLVEGENTLK
                     PVAKLAGYGIYLNRQLNRWVKERIEQNQPLSAEEEAQVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDKNLVKGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEKYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISESTG
                     FDEKNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYADRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADQ
                     KTKLPDSAGRLTEIDIRDYYNAQNMSLQGINILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPDLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSAGRVTSSWKTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(60437..62628)
                     /locus_tag="ACSIGJ_000163"
                     /pseudo
     CDS             complement(60437..62628)
                     /locus_tag="ACSIGJ_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692696.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
     gene            complement(62781..63704)
                     /gene="era"
                     /locus_tag="ACSIGJ_000164"
     CDS             complement(62781..63704)
                     /gene="era"
                     /locus_tag="ACSIGJ_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HHQ1329158.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRLTDADRVVLKQLPKHTPVILVINKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEGDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDNKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(63751..64470)
                     /gene="rnc"
                     /locus_tag="ACSIGJ_000165"
     CDS             complement(63751..64470)
                     /gene="rnc"
                     /locus_tag="ACSIGJ_000165"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HHQ1329159.1"
                     /translation="MKDDVLKRQAHTAIQKKLGYAFRDMSLLRRALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFAERVRRADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            64638..64961
                     /locus_tag="ACSIGJ_000166"
     CDS             64638..64961
                     /locus_tag="ACSIGJ_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HHQ1329160.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADITKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            65008..65484
                     /gene="ribH"
                     /locus_tag="ACSIGJ_000167"
     CDS             65008..65484
                     /gene="ribH"
                     /locus_tag="ACSIGJ_000167"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HHQ1329161.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSQMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVANESGAGIGR
                     VALDYNIPIANAVLTTENDAQAIERIGEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            65562..65987
                     /gene="nusB"
                     /locus_tag="ACSIGJ_000168"
     CDS             65562..65987
                     /gene="nusB"
                     /locus_tag="ACSIGJ_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HHQ1329162.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(66066..67100)
                     /gene="pyrC"
                     /locus_tag="ACSIGJ_000169"
     CDS             complement(66066..67100)
                     /gene="pyrC"
                     /locus_tag="ACSIGJ_000169"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HHQ1329163.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAASVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASENGARFYGIPENADTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(67161..67361)
                     /locus_tag="ACSIGJ_000170"
     CDS             complement(67161..67361)
                     /locus_tag="ACSIGJ_000170"
                     /inference="COORDINATES: protein motif:HMM:NF047650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NMCC_0638 family (lipo)protein"
                     /protein_id="HHQ1329164.1"
                     /translation="MNIELRAEQSVQAPFETRQISYAWRESGSPEETVLTAQTADSPD
                     LPVQAVLNLTHRSHNGKPLILP"
BASE COUNT        16761 a        18963 c        16206 g        15567 t
ORIGIN      
        1 aatgcctccg atataatcgg aggcattttg aattggcacg cccacgggga atcgaacccc
       61 ggttaccgcc gtgaaagggc gatgtcctaa ccgctagacg atgggcgcat attgtatttt
      121 cttactggcg cacccggagc gattcgaacg cccgaccctc tggttcgtag ccagatactc
      181 tatccaactg agctacgggt gcggcacaat cacttgttca agtgattaag aaagcgtgat
      241 aatacggatt tatctgagtc ctgtctatta ttttagactt ctattttcag ttatttttgt
      301 aactgattga ttttaaaata aactattttt cgacgcaagt tgcagcaatt ctgccgcatg
      361 ttgccgcgtc gtatcggtaa taacttctcc gcccaacata cgggcaacct cttcgatccg
      421 ttgtatttca tccaatatac tgatttcgct gacggtttgc tctccctcgc tgtgcttgcg
      481 cacccgccag tggttttctc cgcaggatgc gacttgcgga agatgggtaa cggcaagcac
      541 ctgatgtttt ctgcccaacg cacgtaatgc cttgccgacc atttcagcca ctccccctcc
      601 aataccggta tcgacctcat caaaaatcag ggtgggaact tgggtatatt ggctggcaac
      661 aacctgtaag gcaaggctga tacgcgccaa ttcgccgccg gaggcgactt tgttcagcgg
      721 gcggggcgga ttgcctttgt tagcggcaac ttgaaattga acctgctcca aaccgtgtgc
      781 cgtcggcgac gaaggcaaca ggacgatgtc gaaacgcgcg cctttcatgg caaggtgttg
      841 catatgctcg gtcgtttcgc tactcaaacg ccctgccgcc tgatggcgca tggcagaaag
      901 gatgtgggca gcttcctgat attcggcaaa attgtgggca acattatgct cgagcgcgtc
      961 caaatcagcg gcagcttgaa gactttgcag gcgttcttcg atttctgcca acttggcagg
     1021 caactcttcg ggttcaatcc ggtatttccg cgccatcccc atcagttcgc ccatacgctg
     1081 ttcctgtgcc gccaattcat tggggttgat gtcactgtgt cctgccacat cgcgcatatt
     1141 ggcactgatt tcgcccaatt cggcctcgat gcttgccaac atattcaggc tctcggcaaa
     1201 gcgcggctcg atgttttgca gattggccaa tagtttttga cattgataga tatggcgttg
     1261 gatgccgttg tcgccgtcaa tcttgcttcc gacttcttcg gcggcctgca acaattcggc
     1321 agaatgggca aggctgtcgt ggctttggct gagagcttcc cattcgcctt gtttaatgtc
     1381 caactgattc agttcgttaa actgccattc cagacgctcc cgctcgataa tgacggcatc
     1441 ggcgtgttcc tgcgcctctt ggagggcttt tttcgcattg gcccagtttt gataaagctg
     1501 cctgacggtt tccgcctgca ccctgccgcc cacaaatgcg tccaacaatt cgcgctgggc
     1561 agcttcctga ttaagcgaat gatggtcgtt ttgcccgtgg atgtcgataa gctgcccgcc
     1621 gacggctttg agttgcgcca aggtagccgc ctgattgttg ataaaactgc ggcttttgcc
     1681 tttggcatcg ataatgcggc ggatactgag ttcttccccg ccctcatcca acagcccttg
     1741 ttcacgcaat tctgctttta aagcaggcaa atgggaaata tcgaacaatg ccgacaactg
     1801 cgcttctttt gcgccgctgc ggacttggct gtaatcggcc ttatcgccca acagcagacc
     1861 aatcgcatcc aaagtaatgg acttgcccgc gccggtctcg ccggtcaaaa cggtaaaacc
     1921 gctttgaaaa tccagattca gattttcgac aatgacaaaa tcacgcaaag aaagtgttag
     1981 aagcattgtg aggattcctt agcaattcta ccgacaaaag caatatcaac tttagatata
     2041 cgaattatat ctaagatttt ttaaattaaa aataagtttt tccttaattt tgcttagtgt
     2101 ttgtttttat ctgcttattt gttgcaatgc cgtctgaagc aatgtgcgtt tcagacggca
     2161 tttggaattt cagttgggca gggtatcgga cggtacggtt tccggctctt cttcatccgt
     2221 aggcggcatt tctatcaaat cgggcaaaac cagttcgccc agttcggtca gcggcggcag
     2281 ttcttccaaa ccgtccaagc gcaaatcgct gagaaaagtt gccgttgttg cccacaatgc
     2341 gggttttccc aatgtgtccc gatgtccgat gacttcaatc cacccccgat cctgcaaggt
     2401 ctgcatcacg ttctgcgaca ccgccacgcc gcgtatacct tcgatgtcgc cgcgcgttac
     2461 gggctgctgg taggcgataa tcgccagtgt ttccatcacg gcgcgggagt agcgcggcgc
     2521 acgctgttct tgcaggctgc ccagccgctc gaatgccgtc tgaacaatct gaaaacgcca
     2581 gccctcctgc gtatgcacca gttgcaacgc cctatcctgc caacgcgttt tcaactgcgc
     2641 caacacatca atcagtttgt cttgcgacaa cggcggcaca cacagttcgc gcatagattt
     2701 ttcggtcagc ggttcggttt gggtcagcag tgcggcttca atcagcgcgt cgggagaaat
     2761 tttgtcggtc atacgggtat ccgtgctgaa acggcatggg tttggatatg ccgtctgaaa
     2821 tcggttggag tagagagaag ctgcctgaaa aatatttttc agacggcatt ctttatgctt
     2881 ccgaaggctt ctgccctttg cgctctgctt ttctggcttc gcgaatggct ttgagttcgg
     2941 cttctttctg acgtgctttt ttaagccaag tttcccattg gttcggtgtt tcaagggtga
     3001 ttctgccgat tttgccttcg cggaagtccg tgaggatgtt ttcggcggct ttttggtagt
     3061 tgacccgtcc gccgctgagg actgcgccgc gttttttggc tatccattcg agccacgagt
     3121 tgtcgtccca gtggctgctg gggtctttgt cggcttggta gcgttcttgc agcataggga
     3181 ggtagtggcg gcggaggtag tctaaaagtt cgagggcgac ttcttcttcg tccaatgcgt
     3241 tgcgtccgac tgcgccgccg gcggcgaggt tgtagccgct ttcttcgacg atgattttcg
     3301 gccacagcat tcccggggtg tcgtacagcc agaagtcgtc ggcaaggaaa aggcgttgtt
     3361 cggctttggt aatgccgggt tcgttgccgg ttttggcgga ttttttgcct atcattccat
     3421 tgatgagggt ggatttgccg acgttgggaa tgccgcaaat caaaacgcgc aggggtttgt
     3481 ctatgccttg ccggtgggga atcatcgcgc ggcaggcttg ggtgattttg ccgtgtgcgc
     3541 cggtttcgga ggaatcgagg gcgatggccc gggtgtcggg gcggctgttg tagtggtcaa
     3601 gccagacctt tgtgcgttca gggtcggcaa ggtcttgctt gtttaaaatt ttgagtttgg
     3661 gcttaccttt ggaaagctgc gccaacagtg gattttcgct ggaggcgggc atacgcgcgt
     3721 ccagcatttc aatcaccata tcaacgcttt tggcacgctc ggcgatagct tttcttgcct
     3781 tgttcatatg gccgggaaac cattggattg ccatgtctgt tctttcttct caatatttga
     3841 aatgccgtct gaaacggcgg acggggtttc agacggcata atggtttgac gggattagcg
     3901 gcccgacagg ttttgcgcct gcctgtgccg tatcagcaat tcataacgcc ctttcaacct
     3961 tcgggcgagt ttttcgacaa tatagaccga acgatgctgc ccgccggtac agccgatggc
     4021 gacggtaacg tagctcctgc tttcatcctc caaacgcggc aaccaacgcg taacaaacct
     4081 ttcgatgccg tcaaccattt cctgcgccaa cggctgtccg tccaaataat cccaaacggg
     4141 cttgtccata ccggtgtaag gcctcaactc gggatcgtaa tacgggttgg gcagactgcg
     4201 catatcgaac ataaaatcgg cgttgttcgg cacaccgtat ttgaacccga aggactccaa
     4261 aaccaccagc agtccggtac gctcaacctt cagccactgc cggactgcat ggcggagctg
     4321 ttgggcattc atcttggaag tgtcgataca gtaggcgatt tccttaagcg ggaacagcca
     4381 ttcgcgctct tttttcaggc tttccaacag cgtcatatcc tgattgctca aaggatgtcc
     4441 gcgccgggtt tcggaaaagc ggcggaccag cacggcctct tccgcctcga caaacaaaac
     4501 ttccaccctg tgtccgagtc cgcgcagata ggcaatctgc tcgcgcgctt gggcaatgtc
     4561 tatgccggaa cgcacatcga cgctgaccgc caattcggtt tcgtccgcac gttcgatatg
     4621 gtacgacacc agcgacggca gcatttccaa gggcaggttg tccacgcaaa aatagcccaa
     4681 atcttccatt tggcgcagtg caacggattt gcccgagccg gaaagcccgc taatcaggac
     4741 gattttcatg attctgttcg ttttctttaa gttgcgtctg atggcgttcc aaaaattcgc
     4801 gcgtactgtc tttgccgcgc aactgcaaaa tgtaattgcg taccgccgcc tcaaccaaaa
     4861 cggcaaggtt gcgtccgacg gcgacgggca gcgtaaccga acggacgttg acgttgagga
     4921 tggattcggt ttcggtgcgg atgctcaacc ggtcgagctg cttcatatac tcgtcgtccg
     4981 cctcgaccaa attgataatg agttgcagga tttttttggg gcggatggaa gtttcgccga
     5041 aaatatggcg gatattgagt atccccaagc cgcgcacttc caaaaaatcg cgcagcatag
     5101 gcgaacaacg cccttccaat gtttccggac cgatgcggaa cagctcgacc gcatcgtcgg
     5161 caatcaggct atggccgcgc gaaatcagtt ccaatgccaa ttcgctctta cccaaaccag
     5221 aatgtccggt aatcagcacg ccgatttcaa acacatcgag aaatacgccg tgtttgacgg
     5281 acgatgctgc caaggtgcgt tgcaggtaaa tccgcaacac atccatcaga taagggcttt
     5341 cgagtttgga agtcaacagg gggatgtcgt ttttgtggca gtagtcgcgc agccccgggg
     5401 aaaccggcaa atcgtttgcc acaatgacca aagacatgga aatatcgaac aggtctccga
     5461 actgataacc cgtctccccc gattcgaggc ggttcagata ttccgactcc gccaaaccga
     5521 ccacttggat ttggttggga tggatgaaat tcaggtgtcc gaccagggcg aggacgggct
     5581 tgtccgcctc tacgccgata cggttgtccg cacccgaatt gccggcggcc caggcaagtt
     5641 gcaacttgta ttggttgtcg tcaaacaggc ggcggacgga gatactgggc atattttatt
     5701 cttcggtcag gatggcgcgg gcttcttccg cagaagcaac cgtcatcagc gattctctga
     5761 tgcttttttg ggaaaacttg ccggccagtt tggataagac ttccaaatgc tcgcccgttg
     5821 cgttttccgg caccagcaag ataaaaacca gtgaaaccgg tttgccgtca ggcgcgtcga
     5881 atccgacggg ttcgcgcgta cggatgaacg cgcccgtcgc ctgcttcacg ctcgcgtggc
     5941 ggccgtgggg gatggcgaca ccctgcccca aaccggtcga accgagtttt tcacgggcaa
     6001 aaagacattc gaaaacatca gcatgggaca atgaggattc gcgttccaaa agcaggcccg
     6061 cttcctcaaa cagccttttt ttactgccca cctccatatc caaaacaata tgggacaaag
     6121 gcaaaatttc gccgataagg ctcataagct tctcttttca gacatcgcaa aacagaagga
     6181 ttgtaccgac tgccggggca aacctcaatc ccgcatacgg tacgggctga cataacacag
     6241 cgtttcaaaa acatatttta acgcttttcg gcacagatag aaatgccgtc caaagcagtt
     6301 tacggttctt cagacggcat tgccctgcct tatttcgcga aaaacgtatg ggcaaaaata
     6361 gcggcaaacc acacccaacc gattctgttg tttgccaaaa acgtttcaaa acagatttgc
     6421 cggacgcggc ttttgatggc ggcatattgg cggtattgca gcagcaggac gatggggatt
     6481 gccgtccaat atgcccatgc cgcaccgata accgcaccca atactgccat cagcagggta
     6541 aagccgccat gacacagcat aacggcggcg atgtcgtaac gcccgaacgt gacggcggag
     6601 gttttgatgc cgattttcaa atcgtcctct ttgtccgcca ttgcatagac ggtatcgtaa
     6661 gccagtgtcc ataaaacatt ggcggcaaag agtatccacg cttcaacggg aacgctgttg
     6721 cccactgcgg caaacgccat cgggatgccg aaggaaaagg caagtcccag ataaaattgg
     6781 ggaatcggaa aaaagcgttt ggtaaacggg taagtcagcg caaggaacag cgcgggcagg
     6841 ctcatcagcc atgtcagatg attcagcgga atcaggcaca atgcggcaag cagacacaaa
     6901 aatgccgtca gcagcagggc ttcttttttc ttgaccctgc cctgtgcgaa cggacggttt
     6961 ttggtgcgct cgacagcacc gtcaaaatcg cggtcggcaa agtcgttgat gacgcagccg
     7021 gcactgcgca ttaaaaacgt gccgattgta aacgccgcca ataccgccaa atcgggaatg
     7081 ccgtctgaag ccagccacaa tgcccagtag gtcggccaca gtaaaagcag cgtcccaatg
     7141 ggcttgtccg ccctcatcag tctgatataa acgtccaaac gatcggacag tcgtaaaaat
     7201 aaaggggatt taagattcat aaccctcttt catataagtc ttacaccctt ggaaaactaa
     7261 tccataaact tctagttcca ataaccaatt tgaattattg tcgtaaaatc ttattatttt
     7321 tagaccatct tcttggttct ttatttctac ttggcatata tttctattgt tatgcgacca
     7381 attttgacat ttcattcctt taataaagct atttcttaat tcaatttcaa ctgtatcata
     7441 atcttttagc cacagccccc atttttcgat ttctacagaa ggctttgtac ttgttcggat
     7501 agttaaaata caattagaca agtcagttga aaaagagact agtgaaatat ccattatgcc
     7561 actaggatat atttgtttta gaaaacgatt cctacttatt atttctatta tattcatatt
     7621 tcttactaaa aattatagtg tccgtgagta ccatcgacat gtcctgtatc taaattggag
     7681 tctcttatat taaaatgtgg tttagatgca ggttgccgca cagcttgaaa aaacggtatt
     7741 ttatccgata aagcgtttca gttcgggcag aaaatactcg gtcagcagca tttcctcgcc
     7801 ttgatgggaa aaccgagaac gccgcgcggc aaagtaccgt ccgcatcctt cactggaaac
     7861 ggcaaactca aacgccgaac gcgccccttc caaatcggct tgaaacagac gctcgcccaa
     7921 agggcgtgtg ccgcagtcca aaatgttttg ccaaaacgcc gaaccgacgc tgcacgcgct
     7981 tcgtgcctgc acaacagcag tgccgtccag tttcagaagc acatcgcgca ccctccgccg
     8041 tccgtattcc gtttccgctt cgcccagttt cagcagttcc accgaaaatg tatgcggcaa
     8101 ggcgcacaat gcggcggtca gcgaccgggc tttcaataac cggctcatcg gcaggtcgat
     8161 gccgtctgaa acgggggcgg gcaagtcggg cagccatttc ccaaataggt gttccatatc
     8221 tttcccaatc tttataccgc gcctgttttt gccaactcca tccattccgc ttcgggaaaa
     8281 tcggctttca gacggcattg cagatagttc aggctgtctt gggcgacgtg tgcgtcgcgg
     8341 ctgtcgtgga tgtggttaaa cacgaggctg tgcaggcgaa tgccgtattg tttgagcacg
     8401 acgaaactga gcagggtgtg attaatgctt cccagccgtc cgctggtaac aaggatgacg
     8461 ggataagcct gttgctgaat atgatcaatg gttaacagct tttccgtcag tggtaccatc
     8521 aacccgcccg cgccttcgac caaaacgact tcgtactgcg ccgccaattc ttgtgtggcg
     8581 gtgcggattt tgtccaagtc caaacccctg ccgtccagtc gggcggcaag gtggggcgag
     8641 gcgggatggc tgaagatttc aggcatagtc agacgctgtt catcagcttc ctgcatgggt
     8701 atgcccatga ttttacggtg gacggcgatg tcttcggcga tgtcttggca accggtttgc
     8761 acgggctttt gcgtaatcac gcttttgccc tgctgcaaca attgttttgc caacataccg
     8821 gtggcgacgg ttttgccgat gtccgtgtct ataccgctga cgaagtaaac gcctttcatt
     8881 tgctgtgttc cttcaagatt tgcacggttt tatcggcaag tttggtcaaa atgccgtctg
     8941 aaatgatata gggcggcatc agatacacca gcctgccgaa cgggcgtacc caaatgccct
     9001 gcgccacaca atccgcttga aaacgcgcca tatccacgcc tttttccaac tctatcacgc
     9061 cgatagcacc caaaacgcgc acgtctttca cgccgcgaat gtcccacgcg gctttcagac
     9121 ggccttttaa gatgctttcg atacggcgga tatttgcctg ccagtcttgg gataaaagca
     9181 gcttgaccga agcacaggca acggcacacg ccagcgggtt cgccataaat gtcgggccgt
     9241 gcataaacac gcccgcttcg ccgcgtgaaa tcgtttcggt aactttttgc gaagtgattg
     9301 ccgccgccag cgtcatatag ccgccgctca aacccttgcc gatacacata atatccggca
     9361 cgacctccgc gtgttcgcag gcaaacatct tgcccgtgcg cccgaatccg gtggcgattt
     9421 cgtcaaaaat caacacgata tcaaattcgt cgcacaaatc gcgcaagccg cgaagatact
     9481 gcggatgata aaaatacatg ccgcccgcgc cttgcacgac cggttctaaa ataaaggcgg
     9541 caatatccac atgatgcgct tcaaacaagg cgcggacagg ctgcaaatcc gccccgtccc
     9601 attcatcgtc gaaacggttt ttcggattat cgacaaaata acgctgcggc aacgcgctgc
     9661 cgaaaatatg gtgcatcccc gtttccggat cacaaacgga catcgcgttc caagtatcgc
     9721 cgtgataccc gcgccgtacc gtcgcaatat tctgcttcgc cgtcagaccc cgcgcctgct
     9781 ggtattgcac cgccattttc aacgcgactt ctaccgaaac cgaacccgaa tccgcataaa
     9841 aaatacggtc cagcccctgc ggcaaaatcc cgaccaacaa cttgcccagc tccaccgccg
     9901 gctcgtgcgt caggccgccg aacatcacgt gcgccatttg tttcatctga ttctcaaccg
     9961 cctgattcaa aacgggatga ttgtaaccgt gtatcgcaca ccaccaagac gacatcccgt
    10021 caatcagccg cgtgccgtcc gccaattcga taaacacccc ttctgcacgt ttgacaggat
    10081 aaacgggaag cggatcggtc atggaagtat agggatggag cagatgggta cggtcgaaat
    10141 taagtaatga tgatgtgtgt tgatgttcag acggcataag tttctctctt ttcttattac
    10201 tgcattcaaa cgcaaaaagc gtattctact ccgacagacc gcttcccaca cctttccatc
    10261 cgtttcgggc gaaaaaccgc gaaacaaatc gtccgcaaca taagcgcaca ccgtttcgca
    10321 ttcctcaagc ctgattggaa tcagacgacc caacgcccaa taccgtttca cacagcctcc
    10381 gcccaacgct tcaatcaatc atagataaat acgatacaga tcatagcaac agccatcgca
    10441 acagcgttgg caaaatcagg ggactccggc ataggcgcat agcatctgcc gatgcacggc
    10501 tcctcattcg gctctatgaa taccataccc atcacaaaat ccactgctaa aaccaggcac
    10561 ggcttcttat acttatgata gatttccacc atcctgtccc atatatccca aacactcata
    10621 ccgtatatcc cgcaggcaac aaattccgat tgaaggttac agccctattt tatagtggat
    10681 taaatttata gtggattaac aaaaaccagt acggcgttgc ctcgccttgc cgtactggtt
    10741 tttgttaatc cactatattt tcaaaccgga aaaatgccgt ctgaaccttc agacggcatt
    10801 ccattcatat ttattcgtct tttttgtgtt cgatggcgtt tcgggaacga ttgccgccgt
    10861 aaggctcttc aaccgtatat tctccctcga taatatcgtc atcgtggaaa aatccctctt
    10921 ttctgcccga ttggttcatg ttgaaaaaat tttccgcacc tcctgcctgc aacactgccc
    10981 ctcccttaaa cggcagcagc agcaataccg ccaacaccga ggatacgaat cccggactca
    11041 tcagacacac cgccgccacc gtataacgga taggccacaa catctgataa acagatacct
    11101 tcccactact ttttaccgcc gcgccagcca ataaaagacc ggacagcccc gtatgcctga
    11161 gcatcagcac accggcggca aaggttgccg ccattagaaa cagcgtccaa ccgccgccca
    11221 gccaatcggc aacccacaca atcgacataa tttccaaaaa cagcagcacc aaaaaaccga
    11281 taccgaaaaa tctcattgac cgtcatcctt atatttaagt aaacagcaaa ccgcccgaac
    11341 aggattccaa gcgagctgcc tgtaaatgat tacaaaacca tgtgcttcaa gccgaaacaa
    11401 tgtgaaatca cgcaataatt atttttagcg tgtaaaacaa aaccggctgc atacctgcaa
    11461 ccggtctcaa atcagcacaa ctccttatcc aaatcctcca acaggtcttt cagaccgtct
    11521 ccgatttcct gaagcgtaac cggacggttg ccgtccgaac ccgattccgc accttctgca
    11581 tacggtgcaa acgtgctctt cagtttcaac agtttcacca catccaaacg ggtgtagcgt
    11641 tctccgccgt aaccgacaac cacaccgtga tcatgttgcc attgcgcaaa accatagggg
    11701 ctgatttgca acagtccgca caactcgtcc agcgtgaaat agcgttttgc aggaatcgtc
    11761 aaatcaacgt tgtttgtcat agtagtgttc caccatgcct ttgagtttct ggctggcatg
    11821 gaaagttacc acgcggcggg cggtaatcgg cacttcctcg ccggttttcg gattacggcc
    11881 cgggcgttgc ggcttgtcgc gcaactggaa atttccgaaa ccggaaattt tgatttcttc
    11941 gccgcttgcc aaagtgctgc ggatttcttc aaaaaagagt tcgacaattt ctttggcatc
    12001 gtttttggtg acgttgctga ctttatccac caaaatatcg gccagttctg ctttagttag
    12061 tgtcatatga ttaccttctg ttgtaacagt tgtggattat taccaatttt tatttaaaat
    12121 tcaagcgaat atttattttt taactgcgaa gccgcgcccc tgcctccgtt gccacgccaa
    12181 tcagttttcc aataagcggc tcgaccgcct catccgtcag cgtgttttcc atatcctgca
    12241 aaatcacttt aaccgccacg ctcttcatcc cttcgggcag acccgtgccg cgatacacgt
    12301 caaacacgct gatttcctgt accaacttgt ttgctgcgcc tttcaagaca agcagtaaat
    12361 catcatgatt cataacttcc ggcatcacaa acgccaaatc gcggcgcacc ggctggaatt
    12421 tcgatacggc ccgatagcgc gttttcccgc attccaacac agccgccata tcgatttcaa
    12481 ataccagcgg cgcttgcggc aggtcgtatt tttgcagcca tttcggatgc agttcgccga
    12541 caaagccgat gactttgccg tctgaaacga tattggcggc acgtccggga tgcagggcgg
    12601 gatgtccggt tttaacgaac tcgaccgctt tgtttttcaa cagattttcc acgtccgcct
    12661 tgatgtcgta aaaatccgca ttgcgcgttt tctcacccca ttgttcgggc atcgccgcgc
    12721 cgtaccacag accgccgatg cgttcgtttt ggacaaaccg gccgtctgaa cctttgccga
    12781 acacgcaggc gatttcaaac acgcgcacgc ggttttgctt gcggttcagg ttgttttgca
    12841 aaatttccac taagccgcca atcaacgtag aacgcatcac ggcatattgc gccgccagcg
    12901 ggttttgcag gcggatgggg gcggcgttgg cggcaaaatc gagttcccac tgctcgtcaa
    12961 cgaaggcata gctgatcact tcgcggtagc cgcgcgccgc catttcgttg taaacggcaa
    13021 aacgcggacg gcgtgtttcg ggcagttccg acattttcag acggcctgac gtgtaatcgt
    13081 cggggatgtt ttcatagccg taaacgcgtc cgatttcttc aatcaaatca gcctcaattt
    13141 cgatgtcgaa acggaagctc ggcgcggtaa tgcggaagcc ttccgccgtt ttctcaggct
    13201 gcagacccaa gtgttgcaaa atggtttcca cctgttcggc aggaatgtcc acgcccaaca
    13261 cggttttcag acgacccaaa cgcaatccga cctgcttggc ttcgggcaat ttaccttgtg
    13321 cttccaccat ctcgcctgcc gcaccgccgc aaatctgcaa aaccaattcg gtggcgcgtt
    13381 caatggcatc cgcctgcaaa cggtaatcca cgccgcgctc gaagcggaac gacgaatccg
    13441 aaccgaaacc gtattggcgc gatttgccga cgatgatttc gggctcaaac caagccgctt
    13501 ccagcacgat attttgcgtg ccgtctgaaa ccgcgctcgc cgcgccaccc atcaagccgg
    13561 ccaagctcaa cgcacctttt tcatcagcga ccaccagtgt attgtcagcc aaagtaaccg
    13621 tcttctcatt cagacacgcc aaagtctcgc cattttgagc acggcggaca atcaaactgc
    13681 ccgacagctt atcagcatcg aaaacatgca taggctgacc gatttccagc atcacatagt
    13741 tgccaatgtc caccaatgcg gaaatgctgc ggataccgct gcgttccaaa cgttgtttca
    13801 tccaatcagg agtagccgct ttcgcgttga cattttcaat aacgcggctg ataaaacggc
    13861 cgcagtcagc cggtgcatca atacggacag cctgtttttt ctcactgccg ataggggccg
    13921 tctgaatttc aacaggcgta aacgcacatt gagtcaacgc agaaacctca cgcgcaatgc
    13981 ccttaacact caagcaatca gcgcggttag gcgtaatttt caacgtaaac agcatatcgt
    14041 ccaaatccaa gtattcgcgg atgttggtac cgacaggcgc atcttcaggc agaatgtgca
    14101 ggccgtctac accatcatca ggcaaaccca gttcattggt cgaacacagc ataccgtttg
    14161 atggtacgcc gcgcatttta gtcggcttaa ttttaaaatt gcccggcaaa acggcaaccg
    14221 gcaacgaaca cggcacttta atgcccggct tgacattcgg cgcaccacaa acaatctgaa
    14281 ccaactcgcc cgtaccggca tcaacttgag taacgttcaa acggtctgca tcaggatgtt
    14341 tttcaacaga ttttacttcg gcaacaacca cgccactgaa agcaggggcg gcagtatcga
    14401 tttcttccac ttccaaaccg gccatggtca aaagatgttc cagtttatcg gcagaaagat
    14461 caggattggc ttgggttttc agccatgagt aggagaattg catgttatgt tctcaatcaa
    14521 ttaattaact tttgttttat caattttcgt tcagacagtc tgttttagaa ttctggttaa
    14581 tgttgatatt tactcaactc tgaaaaatat tttctctgtg aaattttaaa aattccagac
    14641 ttgggtttag tctttcaggc agtcttatca tccgattttc atatggttta aataaatcat
    14701 caaatccttc tgtttttgcc agtagtggag aaaatataat tttgaaattt tcatctattc
    14761 ctaacaaccc cctatcaaaa gctttgtcat gtaacgcatt caaacatagc ccattccttg
    14821 ggtttaaacg gttatcctta tcttcccccc agggtttgat atgactagca actaacaatt
    14881 caggttgttt taatccagta atacaacatt gattattgta tgctgccaaa acactggaac
    14941 ggaaaaagcc ctgattgacg cgtactgata ttcttgcaaa cctttccctt ccctttggaa
    15001 tctcaggtga ttctatttca atattactat tctcatcatt tgattggtat tgggaaatga
    15061 ctccctcaaa ttctttatta agttgctccc aatctcctga aaactcattc catacgacaa
    15121 catccatttt actgccatta cttaatcccg aaatattttt gactttcagc gtaggatcta
    15181 aacgacccaa atttcctatc ttcataccga gtgcggacgg tgtcctgccc agtatttcag
    15241 catattcttg aataacagga ttgtccttac taaccttttg aaaaggaatg atgtaataaa
    15301 gataaagggc aaccagggtt tcatctctag tccatttttt catttttatc agctatcttt
    15361 ctgattttcc ataaggaaat cgtacaatac agattttctt attccctgaa catattgctg
    15421 tccatctagt actaatttgt ataggtatac atcataatct tttaactcct tctcgcttgc
    15481 caataaccag tcgtctattt ctgaagaaaa atagtcaata atttcttgta cctgtttatc
    15541 agataaatta tttatattta ttttattatt caaaatataa tcagaaagcc tcatatttct
    15601 aaaatcttta ttttgataag gtaaacccac acagcacatc aaattgcttc ggaatgtatt
    15661 gtttgatttc ttccaaagta atatcgccaa acggcacttc tccgaaattg gcttcgtacg
    15721 tcagtttggc tttttcgtcc cactcgctcg aaaacacgta ttccccgccc aagttctgca
    15781 tcgcgatgcg gaagcccccg atacctgcga acaagtcgat aaaggtaaat ttgggtttca
    15841 aggccgtctg aaaagtatcg ttgttttcaa aaaggcggta ttgtgctgct ttttctttta
    15901 accgttcatc ctgcccatct tccttaatct tgtttttgta aatcaattca tcttgtttgg
    15961 atgatttctt tgactttttc ggcacagcag gttgtatttt tgacgacatc gttactccaa
    16021 aaacgtaaaa ccgtccagcc ctcggctttc agacggcctg ttacttctat atccctttgg
    16081 atattgcgct caattttggc aatccaaaac tcacgatttc cctttattac cgcttttttc
    16141 tgttcccaat ccttaccgtg ccagaattcc ccatcgacaa atacggcaac tttatatttt
    16201 ttaaatgaaa aatcaggttt tccaaaaatg cttccgctat ttttccgata cctgagcccc
    16261 aaagcccaca ttgccttcgc caacacaagc tcgggtttcg tccctgtact tttgttggac
    16321 tgcatacatt ttttgcgctg ctccggggtt aatttatcca ttgggtttat ttgagattta
    16381 ttcaaactgc ttcaagaagt tcaaatcgtt atcgaagaac aagcgcaggt cattgacgtt
    16441 gtagcgcaac atggcaaagc ggtcgagacc aataccgaaa gcgaaaccgg tatatttttc
    16501 agggtcgata ttgacatttt tcaacacatt aggatgtacc ataccgcaac cgcctacttc
    16561 caaccatttg ccgttttctc ccatgatgtc gatttcggca gaaggctcgg taaacgggaa
    16621 gaaagacgga cggaaacgca cttgtaggtc atcgcgttca aagaagcgac ggataaagtc
    16681 agtgaacact gcttttaagt cggcaaaagt tacgccctct tctacccaca aaccctcggc
    16741 ctgatggaac ataggcgagt gcgtggcatc gctgtccaca cggtaaacgc ggccgggggc
    16801 gataatgcgg atgggcggct cttttttatc gagcatatag cggatttgaa tcggggaagt
    16861 gtgcgtacgc aaaacatcgc cgttttcaac gtaaaacgta tcctgcatcg cacgggcagg
    16921 atggtttgca gggatgttca gggcttggaa attgtgaaaa tcgtcttcga tttcaggccc
    16981 gtccgccact tcgaaaccca ttccgtgaaa gagttcgacc acacgttgca aggtcagggt
    17041 tacgggatgc aggctgccgc cttcctgagc gcgtccgggc agggtaatgt cgagggcttc
    17101 ggcggcaagt cgggcttgca gcttggcttc gttgagggca tcgcgtttgg cattaaaagc
    17161 cgtctgaaac cggtttttgc attcattgat atgcgcacct atggttttgc gctcttcagg
    17221 cgacatttgc cccaaagttt tcagaagtcc ggtcaactcg ccggttttgc caagataacg
    17281 ggctttgatt tgttctagag cgttgaagtc ttgcgcagct tctactgcgg caatgccttc
    17341 tgcaacgata cggtttacat tttccataat atcaagcctg tcaattaatg atatgccacc
    17401 tgaaacaacg aggccgcctg tatagcctga catatcagac tcttcagacg accttatttt
    17461 cttcacggcc aatccattgg ataaattttc tgtattttaa cgcaaattta tttaaaaaaa
    17521 tagactttaa agaaagaaaa tcaatcacat agaatttccg caaacaaaga aaaaaggaag
    17581 ccgcagcttc cttttcaatt ttttggatta agcagccaaa gcagctttgg ctttttcaac
    17641 caattgtgca aaagcggctt tatcgaacac ggccaaatca gccaatactt tgcggtcgat
    17701 ttcgatagag gcgcgtttca gaccgttcat aaatttgctg taagacaacc cgttttcacg
    17761 cgcacctgca ttgatacgaa caatccacaa ttgacggaat tggcgtttgc gttggcggcg
    17821 gtcacggtat gcgtattgac cagctttcat taccgcctgc ttggcaacgc ggtaaacgtt
    17881 tttacgacga ccacgatagc ctttggctaa cgcgaagatt ttttggtgac gggcacgggc
    17941 ggtaacaccg cgttttacgc gtggcatatt ctaaactcct taagcgtagg gtaacatttt
    18001 agcaacagaa gccaaatcgc gatcatttac catagaggta ccgcgcagtt ggcgtttgtt
    18061 tttggtggtc tttttagtca agatgtggcg tttgaacgca tgagcgcgtt tcacaccgcc
    18121 gttacccagt actttaaagc gttttttcgc gctagacttg gttttcattt taggcatggg
    18181 aaaactccat tcgttatcgg ataaggcatt agggggtttt aaaacatcgg tttcaaacac
    18241 ttgaacccac aatgccacgg ttttttgccg caattgaaaa cttactccga agcggcaatc
    18301 ggagtaagcg gaaaattata gctttatttt ttcttcggcg caatcatcat caccatttga
    18361 cggccttcca ttttgggaaa ggactcgatt tgcgccactt cagccaaatc ttcttttaca
    18421 cgttccaaaa gttgcgcgcc gagttgctgg tgagccattt cacggccgcg gaaacgcaat
    18481 gtcactttga ctttatcgcc gtcggcaagg aagcggttga tgttgcgcat cttgatttga
    18541 taatcgccct catctgtacc cggacggaat ttgatttcct tgatttgcac ctgcttttgg
    18601 tttttcttgg cttcgtcgcg cttcttggct tgttggtatt tgtatttacc gtaatccatc
    18661 agtttgcaca caggaggttt agcagttggg gaaatctcta ccaaatcgac atcctgccct
    18721 tcggccatag ccaaagcttc acgaactgaa acgacaccaa gctgttcgcc tgactcactg
    18781 attaaacgca cttctttggc ggtaatttca ccgttgattc gtgcttcgcg ttcttgagcg
    18841 atgatgaata ctcctataaa aattaatgat tgacgagggc atcagtgatt tcttgctgca
    18901 attgcgcaat gaaatcatcc aaatccaaag aacccaagtc ttccgctttg cggcgtaccg
    18961 caactttgtt ttcttgtttc tctttatcgc cgataacgat ttgataaggg aaacggtatt
    19021 ggctgttgtc gcggattttg taaccgattt tttcattacg caaatccaac tcggcgcgga
    19081 agcctgccgc ctgcaatttg gcagcaactt cccgacaata atctgcctga ttttcggtga
    19141 tgttcataat caccatttga accggtgcaa gccataacgg gaaagagcct gcatggttct
    19201 caatcagaat gccgacaaac cgctccaaag aacctaaaat ggcgcgatgc aacataacag
    19261 gccgcgctcg gtcgttgttt tctgttacat attctgcatc caagcgttcc ggcaagacga
    19321 aatccagttg taatgtaccg cattgccaag aacgacccaa ggcatctttg acgtgatatt
    19381 cgattttagg cccgtaaaac gcaccctcgc ccggcaattc gccccattcc acgccgcagg
    19441 cagtcaatgc ctcgcgcaaa ccctgctctg ccttatccca cacgtcgtct gaacctgcgc
    19501 gtttttcagg gcgaagggaa agcctgacgg atacatcatg gaaaccgaac tgtttgtaga
    19561 tgcgaaccaa caattcattg aacgcacgag cctcgctgac gatttgatct tcggtacaaa
    19621 aaatatgcgc atcatcctgc acaaaaccgc gaaccctcat cagaccgtgc agtgcgccgc
    19681 tcggctcgtt gcggtggcaa gaaccgaatt ccgccaaacg catcggcaaa tctcgatatg
    19741 aacgcaaacc gttgttgaaa atttgaacat gacccggaca gttcatcggt ttaaccgcat
    19801 attcgcgttt ttccgaactg gttacgaaca tattgtcttt gtagttgtcc cagtggccgg
    19861 atttttccca aaaggtttta tccataattt gaggcgtttt gacctcttta taaccggcgg
    19921 cgttcagctc tttacgcata tgctgctcga tagtccgcca caaagcccaa cctttgggat
    19981 gccaaaacac catacccggc gcttcgtcct gcaagtggaa caaatccagt tgcttgccca
    20041 atttgcggtg gtcacgtttt tctgcttctt caatgcgttg gatatagtct tttaattcgt
    20101 ctttcgtcgc ccaagcagtg ccataaatac gttgcagcat ttcattattg ctgtcgccgc
    20161 gccaatatgc gcctgccagc ttggtcagct tgaagttttt caggaagcgg gtgttcggaa
    20221 cgtgcggacc gcggcacata tcgacatatt cctgatgatg atacataccc attgcttcaa
    20281 cttcgggcat atcttcaatc aggcgcagtt tgtattcttc gccgcgctct tgaaaaattt
    20341 taatcgcctc cgcacgcgga gtcatgattt tgaccacatc ataatcttgg gcaatcaatt
    20401 ctttcatacg cgcttcaatg gcggcaacat cttccggtgt aaacggtttt tccgtggcga
    20461 tgtcgtaata aaagccctct tcaatgacgg ggccgataac catttttgca ttaggataga
    20521 gttgcttgac ggcatgcccg acaagatgtg cgcaggaatg gcggatgatt tcgatgcctt
    20581 cctgatcttt cggagtaatg atttgaacag cagaatcctc aacaatcggg tcgcacgcat
    20641 cgaccaattt gccgtttacc ttgcctgcca ccgccgcctt cgccaaaccg gcaccgatag
    20701 acgcagcaat ttgagccacg gtaacggggg attcgtattg gcggactgaa cagtccggca
    20761 aggtaatatt caacatcaaa cgctccgaaa gattaaaaaa taacggcata aatgccgtta
    20821 tgggaatttg gtaggcacga ttggattcga accaacgacc cccaccatgt caaggtggtg
    20881 ctctaaccaa ctgagccacg tgccttcaaa gaatttttgt attttatcgg gtcgttttaa
    20941 tttttgcaag agagatgtgc atttctttat gcaacattgt ggcaagattt ggggataccg
    21001 tctgaaaaac cgtgggaatt catttataat gccgtctttt aaaatatatt tcgacccctg
    21061 tcgcccaact tacattcaat cttatgctta aatttacttt acacaaaaaa gacggtctcg
    21121 cccgacgcgg cacgctggag ttgaaccacg gaaaaatcga aacgccggtt ttcatgccgg
    21181 tcggtactta cggttcggtc aaagcgatga acccgcaaaa cctgcacgac atcaaggcgc
    21241 aaattatttt gggcaacact tatcatttgt ggctgcgtcc gggtttggag gtcgtcgaac
    21301 agtttggagg gcttcacggt tttatcggct gggacaaacc gattctgacc gactcgggcg
    21361 gttttcaggt tttttctttg tcggatatgc gcaagctgac cgaagaaggc tgtacgttta
    21421 aaagcccgat taacggcgac aagctgtttt tatcacctga aatttccatg aaaatccaaa
    21481 cggttttgaa ctcggatatc gcgatgcagt tggacgaatg cacgccgggc gaaacaacgc
    21541 gcgaacaggc gcgaaaatcc ctgcaaatga gcttgcgctg ggcggaacgg agcaaaaaag
    21601 cctttgaaga tttgaaaaac ccgaacgcgc tgttcggcat cgtgcaaggc gcgatgtatg
    21661 aggatttgcg cgaagaatct ttgcgcggtt tggaggaatt tgattttccc ggccttgccg
    21721 tcggcggttt gtccgtcggc gaacccaagc ccgaaatgta ccgtatgctg cacgccgtcg
    21781 gcccgatgct gcccgaacac aaaccgcatt acctgatggg cgtaggcacg ccggaagacc
    21841 tcgtgtacgg cgtggcgcac ggcatcgata tgttcgactg cgtgatgccc acccgcaatg
    21901 cgcgcaacgg ctggctgttt acccgtttcg gcgatttgaa aatcaaaaac gccaaacaca
    21961 agcccgacaa gcgtccgata gacgaaagct gcacctgcta cgcctgccaa aacttcagcc
    22021 gcgcctacct gcaccatctg caccgcgccg gcgaaatctt gggcgcgcaa ttgaacacca
    22081 tccacaacct gcatttctac caagtcatta tggcggaaat gcgcgatgcc gtcgaacaag
    22141 gcaaatttgc cgactggcag gcgcaattcc acgaaaaccg cgcgcgcggc gtggactgat
    22201 tcccggcaat gccgtctgaa gccgcttcag acagcatttt gccgctttac cctacctgca
    22261 ctatttttca gaaaccgatc atgcttccat tccttccaga accctccctt tcctatacgc
    22321 aacaaaaccg taccgccgtt ttgctgttga acctcggcac acccgacgca ccgaccgcac
    22381 aggcggtacg cccttatctg aaatcctttc tgaccgaccg gcgcatcgtc gaactgccca
    22441 aatggctgtg gtatcccatt ctgcatggcc tggtactgac gttccgcccc aaaaaaagcg
    22501 cgcacgccta tgaaaaaatc tggtttaagg aaggttcgcc cctagaagtt tacacagcac
    22561 gccaagctgc cgcgcttgcc gaacgtatgc ccgacctcat cgtccgccac gccatgactt
    22621 acggcaatcc ttcgatagcg gatgtattgg cggagctgaa atcccaaggt gtaggcagat
    22681 tgcttgcgat tcccctatat ccgcaatata ccgcttccag cagcggagcg gcggtagata
    22741 aagtgtgcga acaactgctg ctgcaacgca accaaatgag cgtccgcacc atttcgcgct
    22801 tttatgatga tgccggctac attgatgcga tgaaaaacca catcctccga tattgggcgg
    22861 aacacgggcg tgggaaaaaa ctgatgttga gttttcacgg cgtaccgcag aagcactacg
    22921 acctcggcga cccctatccc gacgagtgcc gccacaccgc caaactgctt gccgaagcac
    22981 tggaactgac cgaagacgaa tataccgtat cgttccaaag ccaattcggc agggcaaaat
    23041 gggttacacc gagcacgcag gatttgttcg gcaaactgcc caaacagggg gtaaccgagc
    23101 tggacgtatt ctgcccgggc tttttggcag actgcttgga aaccatggaa gaaatcgccc
    23161 tgacggggcg ggaacagttt tatgaagcgg gcggaaaaaa ctaccgctac atcccctgcc
    23221 tcaacgacaa ccccgactgg atagacgcac tcgtcgcact tgccgaagaa aaccttggcg
    23281 gctggcgttg aaactgtttg agccatgtct aaaatgccgt ctgaacgccg tgttcagacg
    23341 gcattttccc gctactttag cgtatgatgg catctatgcc ggacagcaca tcaagtgcaa
    23401 ccttactacc gattcggcaa aaaaaccttt cacccgctac gcaaaatcaa caacaaggtt
    23461 tcaaacatta aggaaacgcc aatgaacaca acccgactgc cgaccgcctt catcttgtgc
    23521 tgcctctgcg ccgccgcttc tgccgccgac aacagcatca tgacaaaagg gcaaaaagtg
    23581 tacgaatcca actgcatcgc ctgccacggc aaaaagggcg aaggccgcgg aaccatgttt
    23641 ccgccgctct accgctccga cttcatcatg aaaaaaccgc aggtgctgct gcacagcatg
    23701 gtcaaaggca tcaacggtac aatcaaagtc aacggcaaaa cctacaacgg attcatgccc
    23761 gcaaccgcca tcagcgatgc ggacattgcc gccgtcgcca cttatatcat gaacgccttt
    23821 gacaacggcg gcggaagcgt taccgaaaaa gacgtaaaac aggcaaaagg caaaaaaaac
    23881 caaacagaca aaatgccgtc cgaaaccggc aatccggctt cagacggcat tcaaaccaaa
    23941 cctttctaac gcggtacttt cagacccata gacccgatat tgccgtcccc gtcaacctcc
    24001 ttcgcggtca gcataaagcc gccgatgtca atacggtcgc cctcgaccgg ctcctgactg
    24061 tcggaacgct tatcgaaaag ctcggcaagg ctcaaaccct cttcgcccgc ttccagcttc
    24121 aaaccgtaag caagtgccaa atcgcccgaa cgtgctgccg gcgaaacgac aaactcaccg
    24181 aagaaatcaa aattttcacg gacgccgata cccgtctcgg taaagtattt cgccatttta
    24241 tcgaccttgc cgtcgggcag gatgtaccac gccaaatcct ccgcctgaag cacggtgtcg
    24301 gactgcattt cgattcggct gccgttgcgt atcaatgcga aacagcgtgc gtcaaacgaa
    24361 tcggaaatcg gttcgaccgt atcgggatgg tgtccctccg cttcagattc ggcaaccact
    24421 ttaaacgccg acattctgac aatttccttt tccgccagcc aaatatcatg cgtatctttc
    24481 ggttcgggct tgttgggcat ggcaaccttc aacagccgcg ccatcacagg aatcgtcgtt
    24541 ccctgaatca gcagcgacag caccaccacg gcaaacgcca catcaaacag caggtgcgaa
    24601 ttgggaacgc ccatcaccag cggcatcatc gccagcgaaa tcggtacggc tcctcgcaag
    24661 cccaaccaac tgatatacgc cttttcacgc aggctgtaat tgaatttcca caaaccgccg
    24721 aacactgcca gcggacgcgc gaccagcatc aggaacgccg caatcgccaa ggcttccgcc
    24781 gccctgtcca acacgccggc gggagaaacc agcagaccga gcatgacgaa caaagttgcc
    24841 tgcgccagcc aagccaaacc gtccatcaca cgcaaaacgt gttccgtcgc acggttgcgc
    24901 tggttaccga caatgatgcc ggcaaggtaa accgccaaaa agccgctgcc gcctatggta
    24961 ttggtaaacg caaacacaag cagcccgccc gacacaatca tcagcgcgta cagaccttcc
    25021 gcaagattca gacggcgtac cagctttgcc aatatctttc cgcccgccca acccgtcagc
    25081 agaccgaaac cgatttgcag cgcaagcatc cggacaaacg ctgccgcacc cgattccgcc
    25141 ggctgcataa tcatggtaat cagtgccgta accaaaaaaa ccgccatcgg gtcgttcgca
    25201 cccgattcga tttccaaagt cgcctgcacc cgttcgttca aacgcacgcc gctgttgcgc
    25261 aaaaggctga ataccgcgcc ggcatcggtc gaaccgacaa tcgccgccat cagcacgccg
    25321 aacttccaat ccaaaccgag ataaaaagtt gcaaacagtc ccagcggaag cacagtggca
    25381 aacacgcccc acgaagcgag taccgacgcg ggcttcaacg caatccgaaa actggaaagc
    25441 tgcgtccgca aaccgccgtc gagcagaata accgccaaag caagctggct gatgaaattc
    25501 gccatcacga cattattgaa ggcaatgccg ccaacgcctt cctcgcccgc cagcatgccg
    25561 acggcaagaa acaccagcag caaaggcatt cccaaacgtg cggacaaagt ggtcgaaacc
    25621 acgctcagga acaacagtgc gccacccagc agaaacaggc tgttcatcca atccacgcca
    25681 acggctcccc cttaaaattc atgaaattag aatcgaaaga ttgtaacacg gcattttctt
    25741 ctcacgaaac tttatttcat gtccgccacc gccttcagac ggcattttcc tgtttcatgc
    25801 cgcctgatac cgcccaaacc cgcgttctcc tatataatgt cggactttgc gccgcatttc
    25861 aggatttttt atggcatttg cctcactatt tactctgccg gactacatcg ccgccgtttt
    25921 ggacgatgtt gccctgatga ccaaagccgc cgcaaagaaa accgccggcg tggccggcga
    25981 cgatttggca cccaatgcca accgggttac aggcgtatcg gcagaacgcg agttgccgat
    26041 tgtttggccg gtggcgaaag gttcttttgc caacaagccg gttttggtgt ctgcggctct
    26101 gctgctttcc gccttcctgc cgcaactcat tacgccgctt ttgacggcag gcgggattta
    26161 tttgtgcttt gagggcgtgg aaaagctgct gcacaagttt ctgcaccgac atgacgcgca
    26221 cgatgacggc ggcgaaacgg cggttaacga gcaggctaaa atcaaaggtg caatccgtac
    26281 cgactttatc ctgtccgcag aaatcatcat catcgcgctg tgcgtggtcg aacaatacag
    26341 cctgatgacg cgctcgctcg tgatggcggc aatcggcatc ggcatgactg ttttggtgta
    26401 cggaatcgtc gccgtcatcg tcaaactcga cgacttgggc atgctgctga tgcgccgccc
    26461 acaacctcgg ccttttgcac gatttcctgc acgcgcaaca ctggaacgct ggctggacag
    26521 agtattccgc caacctcgtt gtcgggccgc tttccggttc gattgcctgc gccccgcctt
    26581 gtcgctgatg aatcgtttcg gcaggcattg attccttttt caaataccga tgccgtctga
    26641 aagacgttca gacggcatat tccgaacaga cagacgaata tgttttccaa actggacaaa
    26701 tactggcagc accccgccct ctactggcct ttgctcatcc tttttgccgc cgccaccccc
    26761 ttcgccttcg caccctacta ccacttttgg ctgatgccct tgattttcgg cgccttcgtc
    26821 cgcctcatcg aactgcgtcc gcgttttgcc gcctcttccg cctacctgtt cggtctgacc
    26881 gcatacacgg cacagttcta ctggatacac actgccctgc acgacgtttc cggcctgccc
    26941 gacctctatg ccgtaccgct gaccttcctg ctgcccgcct accttgccct ttatccggca
    27001 ctgtgtttct ggctgtggaa aaaatttacc ctgccccagg gcataaagac cggtctggta
    27061 ctgcccatcc tgtggacgct gaccgagttt gcccgcgagc gtttcctgac cggattcggc
    27121 tggggcgcaa tcggctactc ccaaatcacc ccggacagcc cgctggcggg tttcgccccc
    27181 ttgggcggca tccacatggt tacactggca accgcctttc tcggtgtctg gctggttttg
    27241 gcgatcgaca acaccgcacg ttcgggcaaa cgcctgcttc cggccattct gattgccgcc
    27301 ctgcttgccg caggctacac cgcccaacaa accgacttca cccgccccga cggcagccgc
    27361 agcaccgtcg ccctgcttca gggcaacatc gcccaaaccc tcaaatggcg tgaagaccaa
    27421 gtcatcccga ccatacagaa atattacgaa caggtcggca aaaccactgc cgacatcgtc
    27481 atcctgcccg aaaccgccat tcccatcatg cgccaaaacc tgccggaaaa catactggcg
    27541 caattcgccg aacaggcgca aaacaacggc agcgcgctcg ccgtcggcat cagccaatac
    27601 acttcggacg gcaacggtta cgaaaacgcc gtcatcaacc tgaccggcta tcaggaaaac
    27661 aatcaggacg gcatccctta ctacgccaaa aaccacctcg tccccttcgg cgaatacaaa
    27721 ccgctgcccc tcctgaccac accgctttac aaaatgatgg acatgcccct ttccgacttc
    27781 cgcaaaggcg gcggcaagca atccgccatg atgatgaaaa accaaaaaat cgccttcaac
    27841 atctgctacg aagacggatt cggcgacgaa ctgattgccg ccgccaaaga cgccacactg
    27901 cttgccaatg tcagcaatat ggcgtggtat ggaaaatcca acgccatgta ccagcacctc
    27961 caacaatcgc aggcgcgggc tatggaactc ggacgctata tggtccgcgc caccaacacc
    28021 ggcgcaaccg ccatcatctc ccccaaaggc aacatcatcg cccaagccca acccgatacg
    28081 gaaaccgtat tagaaggaca catcaaaggc tacatcggcg aaacgcccta tatgaaaacc
    28141 ggcagttcat ggtggttgat gggcatattg accctagccg cactgattct tttcatcttc
    28201 cgaaacaaag aacactgata cctgcacagt ccgtccggac gggctgtctt ttttcaaacc
    28261 ggccgtttac atacaaccac gtatgtaata taattctaaa atcgaattga gctatcaggt
    28321 ttacaaaatc aatcaaactt gccttacaaa attttcctgt ctttcaaacc ttagcgtata
    28381 tctcctgcca aacatacaaa atacaagcca agcagtgata tactgcatcc caacacacca
    28441 aaccgccgat acgcagtttc agccgaaagg aaatgcccca aatgaataac gctttcgcat
    28501 tacccgccat ccaaagcggc aacggcagcc tcgaacaata catccacacc gtcaacagca
    28561 tcccgatgct gtcccaggaa gaagaaaccc gcctcgccga acgccggata aagggcgacc
    28621 tcaacgccgc caaacaactc atcctgtccc acctgcgcgt cgtcgtttcc atcgcgcgcg
    28681 gctatgacgg ctacgggctg aatcaggccg acctgattca ggaaggaaat atcggtctga
    28741 tgaaggcggt caaacgctac gagcccggtc gaggtgcacg cctgttttca tttgccgtgc
    28801 attggattaa agcggaaatt cacgaattca tcctgaggaa ctggcgcctt gtccgcgttg
    28861 ccaccaccaa accgcaacgc aagctgtttt tcaacctgcg cagcatgcgt aaaaacctta
    28921 atgtcctgtc tcccaaagag gcacaagaca tcgccgacga tttgggtgtc aaactttccg
    28981 aagttctgga aatggaacag cgcatgacgg gacacgacat cgccatcatg gcagacaaca
    29041 gcgacgacga ggacagtttc gcccccatcg actggcttgc cgaccacgat tccgaaccga
    29101 gccgacaact atcgaaacag gcgcattacg ccctgcaaac cgaaggcctg caaaacgcct
    29161 tggcgcaatt ggacgacagg agccgccgca ttgtggaaag ccgctggctt caagacgacg
    29221 gcggactgac gctgcaccag cttgccgccg aatacggcgt atctgccgag cgcatccgcc
    29281 agattgaagc gaaagccatg cagaaactgc gcggtttcct gaccgaagaa gccgaagcgg
    29341 tttgaaatat acacaataaa atacccgaca aaacaatttg tcgggtattt tatttggtaa
    29401 acatcaatgc ggtttgtgcg tcgtgccgta cacgatttta tccgcccaag ccatcgcaat
    29461 cgccaaaatc aaaaagcaga catgcaccag acactgccac atcagctgct tttccgacag
    29521 attggcggaa ttgataaatg tttggagcaa atggatggat gaataccgat aatcgacatc
    29581 gacagcctta ccttcaatac cggtgcattc acatggctca accactcggg ccggtcagga
    29641 tggtcgtcga tacgcaaccg ggatacgaac gactcatacc cgccaatctg caccatggtc
    29701 agcaaattcg caatcataac cacatcaatc agatttaaaa cagcgagcat gatggcgttt
    29761 tcgtccgaca catccaaatt catgaccaga tgcttcaacg atttcaaaaa cttataggca
    29821 caaatcgccc gtacaacagt cagccccgca taaatcggca cttgcagcca acggcttgca
    29881 aaaatcattt tggcaaaaaa cccatagcct gttcgggttt tggagatttt gcttgataca
    29941 tttcccttcc ttgcagcaac aaaagcgcgt gattccaatc taccgctgcg aaatcttcaa
    30001 tttttcacac ccgaaaacgc ttgccgccat acagatgtga tatattgatt ccattttaac
    30061 gaacttcgga gaacgacatg agcagcggaa atatccgcat ccacagcatg accggctttg
    30121 ccaacgcggc ggcagagtgc ggcagcaaac gcatcaacct cgacatccgc gccgtcaacc
    30181 accgcttttt ggacattcaa atcaggatgc ccgacgattt gcgccatttg gaaaacggca
    30241 tccgcgagca gatttcatcc catctggcgc gcggcaaagt cgaatgcaaa atccaaattc
    30301 aagatgcgga aaacggcaat cagtctttgg aactgaatcg cgttctggtc ggacagcttg
    30361 ccgaaatcaa caaaaacctc cgcaaacatt atgatttggc aaaactgggc gttgctgata
    30421 ttttgcgctt tccgggcgta ttggcaagcc aaaaggaaaa tacggaagca ttggcgaaaa
    30481 ccattgtcga actggccgac aaagcactaa aagactttac cgccgcacgt cggcgtgaag
    30541 ggaaaaaatt gggcgaacac ctgttacaac gcattaaaaa catggaagaa atcatcgatg
    30601 ctttgagcga aatcttcccc gcgctggtgg aagcgcataa agaaaaaatc cgcacccgcc
    30661 ttgccgaagc cgtcggcagc atcgacaacg acaggctgca acaggaattt gccctcttta
    30721 tccaaaaatc cgacatcgac gaagaattca gccgcctgcg cacccacatt gccgaagtgc
    30781 gccgtatcgt taccgaacac aaaggcagca gcggcaaacg gttggacttc ctgatgcagg
    30841 aactgaaccg cgaagccaac actttgggca gcaaatccat cgctaccgaa tgcacccaag
    30901 cctcggtcga gctgaaagtc ctgatcgaac agatgcgcga acaggtgcag aatatcgaat
    30961 agccctgcct ttctctccaa acatgacaat gccgtctgaa acttttttca gacggcattc
    31021 ttgatcggct gaaaacctct gttgcaagca attcaagatt gttctttcac atccaccaac
    31081 ctcgttccgg caaggaagtc atacagaaac tgccggtcgg gattcagcag ggcgaacccc
    31141 cacggcaaaa tcagccaaac caatgccgcg ccggccgcgc ccttgggcgg aatgccgagg
    31201 aagtggcgca atccggcata agcaagcata ggaatgaaga cgatgaatat gcacgcccaa
    31261 ataaagcgca ggcgcagcag gtgcaaagac ggctgtatgc cgttaaggtc gcacaagccg
    31321 attttccatg tcctcatcgc caaggtctgc ccctgaccgt gccagttggc gcggaaataa
    31381 agccaccatg ctcccattat caggatactc gttaccaatg cagaaaccgc gatagaaacg
    31441 ggattcagaa aaatggcggc aataccggca agcaatgctg ccaaacaggt cgccgcaccg
    31501 accagcagca tctcatacag tatggcggca aagcggcgtt tgagcggggc ggttttcagt
    31561 atcatcatct tttcaatcgg aatatgccgt ctgaaacgcc aacagacttc agacggcatt
    31621 tgtgcaaaga tgccttgcgg cggttaacgg tgcggacgtt tctgaccgcg cgactgtttt
    31681 gccgccattt gattgatttc ctgacgtgtc gggcgttttt tgaacatagc gtgcgcctcg
    31741 tccaccgaac atttttgcga caggcagatc agggtaatcg catctttctg gtgttgtccg
    31801 ccgctgtggt acatacggcg caggtttgcc tcgttgattc tgcctttttc gggatcgatg
    31861 tcgaattttt tcagacgttc gataaactgg cgttgctgtc ggcgggatgt ccaaacaaag
    31921 ccgcccgcag cggcggcgac gatgacggcg gcaagcagga tggcaagcgg ccaggaatcg
    31981 acaatcaata cgataatgag gcttaaaacg gcggtggtca gtaaaatcag gccgcccaag
    32041 cgcagtttgg tcttattgtc catagggttg atgttccttt attgaggtgg tcgtgccgtc
    32101 tgaaatgttt tcggacggat tggtgaatac ttttcaggat taacggtatt ttacagtatt
    32161 ttcggatttt ggggattacc gctttattcc gccagcgaca cgacaagctg tttgaatacc
    32221 gtccatgcgt cgccgtcttc cgcgcctttg atgattcggt cgatttgggc gcaggttttg
    32281 agcgcgtcaa gcaggcggac gacggaaatc cgcttgaccg caagcggtgc gagcgtctgt
    32341 ttgtcgcccc aaagcctgag gctgttgcgg acggattgga tgctctgccc ctgtttcagg
    32401 gcagcggcaa gccgaatcag cgtccgcacg tcttcggcaa ccgcccacag aagcagcacc
    32461 ggttcttcgc cttcttcctc caacccgtcc aacagcctgc atacgcgcgg gacatcgccc
    32521 ttcatccacg cgcctgccag ttggaacgcg tcgaagcggg caacattggc aacggcggtt
    32581 tgcgcctcat cgatattgac ggcatgccct ttcggataca gcagcgcgag cttgtcgatt
    32641 tcctgacgtg ccgccaacag attgccttcc acgcgctcgg caaacagtgc caatgcgtct
    32701 gcctcgatac ccaaaccgat tttgtccagc cgtccgcgta tccattgggg caaagccgcc
    32761 gcaccgaccg gtttggcttc ccatacttcc ccctttgccg ccaatgcggc aaaccatttg
    32821 gactggagcc gggttttctc cagtttgggc agcaaaacca gcgttaccgt atcttccggc
    32881 agtcgggcgg caaaatcctg caatgcctcg ccgccgtttt tgccgggttt tccgttgggg
    32941 atatgcaatt ccaacagttt caaatcggca aacagacccg cactgcctgc ggtttgcagc
    33001 agttcgttcc aatcgaaaga cgcgtcggcg gtataggctt cgcggttgag ataaccctgc
    33061 ttcttcgccg ccgccctcaa tgcgtccact gcctcgatac gcagcagttc ttcctcgccg
    33121 tggatgacgt acagaggttt caaaggcgcg tccgtatcga tgcgtccgat atgtgccgcc
    33181 acgtcattcc gccttcagaa aggtcaggcg gcggacaatc tgttcggcaa catcctgccg
    33241 catttccgcc cacagggttt cttcttcttc ctgtttgccc aaaatttcgt tgtcggcata
    33301 atccaaaatg cggcggacgg acacggtcat cggtttgccg accggctcgc cgcgtttcaa
    33361 tacctgcgct tcaaccgtca atatcaaaag atattcgttg atgactgccg cacgggtaac
    33421 ggtataggtt tccttgtttt gggaaacgct gtctatacgc agggtcatct gcgcgccggc
    33481 agcatcgtcc accctgcccg aagcctgata cagcgcggtt tccaaaggaa actgcaatgc
    33541 ctgtccgcct tcgatgtgcc agctccggta ggtcagcggc ggagaaatgc cgtctgcacc
    33601 tttcaggtgg aaaccgcacg cgcccagcat caaggctgcg gcagtaagga atattttgtt
    33661 catcgcttgc ctttctcaaa agcataaagt cggtagatta tatcagcggc gtgttcagac
    33721 ggcattgccg tccagccaaa tcaggctcgc catacgcccc gtcgctccgt cgcggcggta
    33781 ggaaaagaaa atatcccgtt ccaaaaccgt acaatgcgtg ccgccgtaca cgccgcccac
    33841 gccttcgcgc ttcagaatca ggcgggcgag cgcgtagagg tcggcaagga atttgccgcc
    33901 gccgatgggt tcaaatgcag cagcggcttc gggcatggac gtgcagaacg catcaaacac
    33961 gtcctgcccg acttcaaacg catccgcact gatggcaggg ccgagatacg ccataatttc
    34021 gacaggcgga accttcatcg cggcaatggt gttttgcaat acgccgcccg ccaaaccgct
    34081 ccagcccgcg tgtgcggcgg caaccgccgt acctgccctg tcgcaaaata gaacgggcag
    34141 acagtctgca gtcatcacgg cacagacggc tttacccgtg tcgtccacgg aagcgtcggc
    34201 atcgggcgtg ccgtccaatg cttcggcagc attgacgaca atggtgctgt ggatttgatt
    34261 gagataggca acgggcaatc cgacctgctg ttgcacgatt tcgcggttgc ggcgcacggt
    34321 ttcagggtcg tcgccgacgt gcgtaccgag gttcaaactc tgatacgcac cttggctcac
    34381 gccgccgttg cgcgtggtaa tcagggtttt cacattggcg ggcgcgggcc aatcggcggt
    34441 caggaagttt ttgcctttcg gggcgagatt tagggtttct gtgatggttt tcattggata
    34501 ttggaatagg tgtgcggcgg aaagggttga ttaactgcct tcggggcaaa tggatgcttg
    34561 ttttttcgtc attcccgcac aggcgggaat tcggagattc gggattgttt tcaaacgttc
    34621 gggtttggat tcttgccggt gcgggaatga tgcccttata tactttctcc gagctttata
    34681 tgcttcaaca ggggacggca catcaagcac cgcactgcgt gttgcccgaa cgaggctgcc
    34741 ctccgattag attctatcgc tataaacaga cgggtttcaa ccgaaaagga atggggataa
    34801 agtccatttc cgacacctct cgggcgatgc cgtctgaaaa ccaatctcca ctttcagacg
    34861 gcattgttta ggcgcggtca ttttgcaaat taaggttaag gcgtgtatgc ttgccttccc
    34921 ttaaaaacaa acacgcaacg cattattatg aatatcctca gtaaaatcag cagctttatc
    34981 ggaaaaacat tttccctctg aacctcgctc tttgccgccg ccgctttttt cgcgcccgac
    35041 accttcaaat gggcggggcc ttatattcct tggctgttgg gcattattat gttcggtatg
    35101 ggtttgacgc tcaaaccttc cgacttcgat attttgttca aacatcccaa agccgtcatc
    35161 atcggcgtaa tcgcacaatt cgccattatg ccggcaaccg cctggctgcc gtccaaactg
    35221 ttgaacctgc ctgccgaaat cgcggtcggc gtgattttgg tcggctgctg cccgggcggt
    35281 acggcttcca atgtgatgac ctatctggcg cgcggcaatg tggctttgtc ggttgccgtt
    35341 acgtctgttt ccaccctgat ttccccattg ctgactcccg ccatcttcct gatgcttgcc
    35401 ggtgaaatgc tggaaatcca agcggccggt atgttgatgt ccatcgtcaa aatggttttg
    35461 ctccccattg ttttgggttt gattgtccat aaggttttgg gcagtaaaac cgaaaagccg
    35521 accgatgcgc tgccgctggt ttccgttgcc gccatcgtgc tgattatcgg cgcggtagtc
    35581 ggggcaagca aaggcaagat tatggaaagc ggcctgctga tttttgcggt tgtcgtactc
    35641 cgcaacggca tcggctacct gctcggcttc tttgccgcca aatggaccgg cctgccttat
    35701 gatgcacaaa aaacgctggc catcgaagtc ggtatgcaaa actcgggcct ggccgccgcg
    35761 cttgccgccg cacactttgc cgccgcgccg gtcgttgccg ttccgggcgc attgttcagc
    35821 gtgtggcaca atatctccgg ctcgctgctg gcaacttatt gggcggccaa agccggtaaa
    35881 cataaaaaac cctaaacgcc tttcatcata atgccgtctg aacggggcaa ccgaaaccgg
    35941 cggtttctgc ccggcccctt cagacggcct ttgtttgaaa tcggcgggga cgattgcccg
    36001 gctgctccgg ttcggctgcc tgcgttcgcc ccgcctttca agccgcctca ctcccttaca
    36061 taaaccactt cgacatcgta gtcgtcttcg ttccaatcgt catcgtcgtc cgcgccgaat
    36121 ttgtcctgcc attcctcttc attgctcaaa gacgaatcca aaccggcttc aagacggagg
    36181 acggacaaca aatgatatat gtcgtccgga atcggtgcct caaacgaaac ggtttcgccg
    36241 ctttccggat gggtaaaact caagcggtag gcgtgcaacg cctgacgcgc gcccaaactt
    36301 ttaacggctt ctttcaccgt gtcgccgcac ggatggcgcg ggttgccgta aaccgggtcg
    36361 ccggcaagcg gatggttggc ctcgcgcata tggacgcgga tttggtgcgt cctgcccgtt
    36421 ccgagcgagc attcgatgta gctgtgggca agatagcgtt ccaacacttt gacgtgggtt
    36481 acggcaggct tgccgccgaa tttgacggct gccattttca ggcggttgtg cggatcgcgt
    36541 ccgatttggg tttcgatttt accgtcgaag gggacgatgc cgttggcgac ggcgcggtag
    36601 atgcgtttga ccgtgcgttc ttgaagctgc ctcacgaggg aattttgcgc cggcagggtt
    36661 ttggcaacca ccatcagccc gctggtttct ttgtccaaac ggtgtacgat gcccgcgcgc
    36721 ggaatttggg acagctcggg gcaatgcgcc aacaggccgt tgagcagcgt ccccgtccag
    36781 ttgcccgccg ccggatgcac caccagtccg gccggtttgt tgacgacgat gacggtatcg
    36841 tcttcgtaaa caatatccaa atccataggc tccggaacaa acgccagatt ttcctcactc
    36901 ggacggacgg ttacacaaat ttgctcgccg cctatcattt tgtctttggg ttgcgaaggt
    36961 ttatcgttta caataaccgc gccttcttta atccatgatg tcaggcggct gcgcgagtag
    37021 tcgggcagaa gtttcgccaa caccgcatcc aaccgcccgc cggcaagctc gggcggaacg
    37081 gtcagaccga tacggttttc agttccggaa gctgacgcaa agtctgaatc gtcgctataa
    37141 tcggattcat tatcaaagga agtattctgc atgaaaaaaa ttcttttaac ggtttcatta
    37201 ggtttggcac tgagtgcctg tgccactcaa ggtacggccg acaaagatgc ccagattacc
    37261 caagattgga gtgtggagaa gctctatgcc gaagcccagg acgaattgaa cagcagcaat
    37321 tatacgcggg ctgtcaagtt atacgaaatc ttggaatcgc gcttccccac cagccgccat
    37381 gcccggcaat cccaactgga taccgcatac gcctattata aagacgatga aaaagacaag
    37441 gctctggcgg caatcgaacg cttccgccgc ctccatccgc agcatccgaa catggattac
    37501 gcgctgtacc tgcgcggctt ggtgctgttt aacgaagatc agtccttcct gaacaaactg
    37561 gcctcgcaag actggtccga ccgcgacccg aaagccaacc gcgaagcgta ccaggcgttt
    37621 gcggaactcg tccaacgctt cccgaacagc aaatacgccg ccgatgcaac cgcacgcatg
    37681 gtcaaactgg tcgatgcttt gggcggcaat gaaatgtcgg tggcgcgtta ctacatgaaa
    37741 cgcggcgcat atatcgccgc cgccaaccgc gccaaaaaaa ttatcggcag ctatcaaaat
    37801 acacgctatg tcgaagaatc actggccata ttggaacttg cctacaagaa gttggacaag
    37861 ccccagcttg ccgccgatac gcgccgcgta ttggaaacca acttcccgaa aagcccgttt
    37921 ttgacgcacg cttggcagcc cgacgatatg ccctggtggc gttactggca ttaagcccat
    37981 cctcgaaatg ccgtctgaag ccctttcaga cggcattttc cgttccggca ccagccttcc
    38041 cgttcactca aacggttttt tctgccaata gaatttatac actctcaaac gttgagcctt
    38101 tacgcctccg cgtcccaagc cgaattgcag cgcgcccgac aaatccgtcc gcaacaactt
    38161 gatgccgtgt gcgcggacac ggttctgcac cgcttcggtc ggatgcttgt aggcgttggc
    38221 ataaccgctt gaagcaacgg catattcggg cgaaacggca ttgaggaaca cgcccgacga
    38281 ggacgtattg ctgccgtgat gccccaacac caacacctgg ctgtacaggt tgcctccata
    38341 cttgccgacc aggctttcct cgcccttcgt atccaaatcg cccgttacca gcagtgccgc
    38401 accgcccgcc acaacacgca aaacacaact tttcccatta tcatcgatat ttttgcgttc
    38461 agacggcctc aaaaactcga aatccacgcc gtcccattgc caacgctgtt ccgcacaatg
    38521 ccgcgccccc tcataaaatt ccggctgtcc ggcataaatc ccgccgttcg gtatcttgcc
    38581 gactgcctga aagccgccgt cgtggtcgct gtcgtgatgc gacagaacca gcttgtccaa
    38641 acggcggaca cccgccgcat tcaaactcgg cacgataccc gtttgtgccg ccgctaccgt
    38701 tcccgtatcg aaaagcagat ggcggtttgc cgtccgcaca agcaccgaca agccctgccc
    38761 cgcatcccaa accgtaaccg cagcttcgtt ttcaggtact gcttcaggac ggtaggatac
    38821 aaaccctgcc aacagcaaca ccgcccacgg acgcaaaccc aagccgcgcg gcagcagcaa
    38881 cagcaaagcg gcacacaccg ccaacaccag cagcggcaaa ggtgcggcgg caacggcaaa
    38941 ctcaggcgac acatcggcaa gccacaccaa aaaccgcaaa gtgtattccg ccaaaaatgc
    39001 ccccgcctgc tgaagcggcg caaacggcac gaccgaaccc agcaacgcca gcggggtcaa
    39061 cacccaagaa aaccacggta tcgacaccgc attgaccaaa gggctgacca aaggcagcga
    39121 ggcaaacaga taaccgagca aaaccagaga caacaccgaa gccgcccact gcccgcgcac
    39181 ggcggtttgc cgtttcccct cgtacaaacg ccctgcacac gcccatatca gggccgccac
    39241 caaaccgaaa gacagccaag taccgacacc caaaaccgcc aaagggtcga acagcagcac
    39301 cgccgccaac gcctgccacc acgtcgccca cgccgacagt cttcccctgc gccacgccca
    39361 cgcgaacgcc gccaacatca aaacactgcg ctgcgtcggc acggaaaaac cggcaagcag
    39421 cgcgtaaaac agcgcgcccg cacaccccgc cgccaaaacc cacgcgcgcg gccgggcggg
    39481 aaggcgcggg gaacaggcaa gcagccgctt cgccagccac gcaaacagca ccgccaccat
    39541 cgtaacgtgc aaacccgaga tactgaccaa atgcgtcagc cccaacggcc ggaacgcctg
    39601 ccacaatccg gggcgcaatg ccgactgttc gcccacgctc aacgcgcgca tcagcccgat
    39661 gccgtctgaa agcccgccgt ccgcatccgc ctgccgccaa ttgcggctga tgcggctgcg
    39721 ccaaaccgca atcccccaac cgcttccgcc atgcagcaaa accctgtccg caccgaccgt
    39781 ccccacgccg cctaccccgt tggataatgc ccacgcctca cggttcaaac cgcgcaggtt
    39841 caactccccg acgacggggt gcacgcgtgc cgttatccgc catctgctcc cgaccgccca
    39901 ttcgcgccgt ttgtagtccg acagcagcaa atcaaacgtc cgaccgccgc tgtccacagc
    39961 ctttgccgca aactgcacgc gccgcccgtc cgacctcggc atatccgtca cttccaccgt
    40021 caaaggcacg cctgaaaccg cctccgcccg ccattgcgaa gacagtgccg cttccgttct
    40081 gaatacgccg taagccgcgc ccgccaacac gcacagcatc agaccggcaa acgcaaaacg
    40141 ccttgcaagc acagccaaaa ccgcaaaagc cgccaaccaa aacggccaat gcggtacaac
    40201 cgacagtgcg aacgaaactg ccacgccgac aacccaacac ggcaaaaacc aacgacggaa
    40261 cacgtctccc cactcccgat ataaaaaaca aacgatgata aagcaaagcc ataaaaccgt
    40321 cataacaaga tttgaaaatg ataagaatgc aaacgaaaag cctcagtatt atccgagaca
    40381 acggcaaact ttccatcgtc attcccgcaa aagcgggaat ccaaagacaa aaagccgcag
    40441 aaatttatcg gaaagaaccg aaccacaaaa accggcttcc caccttcgct tgaataacag
    40501 aacaaagaaa gcggaaataa aaacatcagg attctttttt aaattggcaa taaaacacca
    40561 aaatgaatga gttacacaaa aaaatactca acaccaccca accggcgtaa aatgcaaaac
    40621 attatcgcta ttaaaacggt aaaaccttat gaaagccaaa cgttttaaaa ttaacgccat
    40681 atccttatcc atctttcttg cctatgccct tacgccatac tcagaagcgg cattggtgag
    40741 agacgatgtc gattatcaaa tattccgtga ctttgcagaa aacaaaggca aattttttgt
    40801 cggcgcaacc gatttatcag tgaaaaacaa acaaggtcaa aacatcggca acgcgttatc
    40861 caacgtaccg atgattgatt ttagcgttgc agatgtcaac aaacgcatag caacggtagt
    40921 cgatccccaa tatgccgtca gcgtcaaaca cgccaaagca gaagtccata ctttttatta
    40981 cggccaatac aacggtcata atgatgtagc cgacaaagaa aatgaatacc gcgttgtcga
    41041 acaaaataac tatgaacccc ataaagcttg gagtgcgagc aacttaggcc gcctcgaaga
    41101 ttataatatg gcgcgtttta ataagtttgt aaccgaagtg gcaccgattg caccaactga
    41161 tgccggcggc ggattagata cttacaaaga taaaaaccgt ttttcctctt ttgtcagaat
    41221 cggtgcgggc aggcaattag tttatgaaaa aggggtttat catcaagaag gaaatgaaaa
    41281 aggctacgat ttgcgcgatc tttcacaagc ctatcgttat gccattgcag gtacgcctta
    41341 taaagatatt aatattgacc aaacaatgaa taccgaaggt ttgatcggct ttggtaatca
    41401 taataaacag tattcggcag aagaacttaa acaagcactt tcgcaagatg cgttaacaaa
    41461 ttacggcgtg ttgggcgata gcggctctcc gttatttgct tttgacaaac aaaaaaatca
    41521 atgggtcttt ttgggaactt acgattattg ggcaggttac ggaaaaaaat catggcaaga
    41581 atggaatatc tataaaaaag aatttgcaga taaaatcaaa caacacgata acgccggcac
    41641 cgtcaaaggt aatggagaac atcattggaa aaccacgggt acaaacagcc atatcggttc
    41701 gacagcggta aggcttgcca acaatgaagg agatgcgaac aacggacaaa atgttacctt
    41761 tgaagacaac ggcactttgg tattggatca aaacatcaac caaggcgcgg gcggcctgtt
    41821 tttcaaaggc gattacacag tcaaaggcgc aaataatgac atcacttggt taggtgcggg
    41881 gattgatgtt gccgacggca aaaaagtcgt ttggcaagtc aaaaatccga atggcgacag
    41941 attggcaaaa atcggcaaag gcactttgga aataaacggc acaggcgtta accaagggca
    42001 attaaaggtc ggcgacggta cggttattct gaatcaaaaa gccgatgccg acaaaaaagt
    42061 tcaggctttc tcccaagtcg gcattgtcag cggacgcggt acattggtat taaatagttc
    42121 aaatcagatt aatcccgata acctatattt cggtttccgt ggcggtcgtt tggatgccaa
    42181 tggcaatgac ttgacttttg aacacatccg caacgtggat gaaggcgcgc gcattgtcaa
    42241 ccacaacaca gaccacgcct ccacaatcac actaacgggt aaatctttaa ttaccaatcc
    42301 gaacagcctt tcggtacata gcatacaaaa tgactatgat gaagatgatt atagttatta
    42361 ttaccgacca aggcgaccca tcccacaagg caaagatctt tattacaaaa actatcgtta
    42421 ttacgcccta aaatccggcg gcaacgtgaa cgcaccgatg cccgagaacg gtgtagcaga
    42481 aaataacgac tgggtcttta tgggatatac gcaagaagaa gccaagaaaa acgcgatgaa
    42541 ccacaaaaac aatcagcgta ttagcggttt tagcggtttc tttggcgaag aaaacgaaaa
    42601 aggacataac ggggcattaa accttaattt caatggcaaa agcgcgcaaa accgtttctt
    42661 gttaacaggc ggcgcgaatt taaacggaaa aataagcgta actcaaggca atgtcttgtt
    42721 atcaggtcgt ccaacaccac acgcaagaga ttttgtgaac aaatcttcag cccgaaaaga
    42781 cgcacatttc tccaaaaaca atgaagtcgt atttgaagac gactggataa accgcacatt
    42841 caaagccgca gaaattgcgg ttaaccaatc cgcatcattc tcttccggaa gaaatgtatc
    42901 cgacatcacc gccaacatca ccgcaacaga caacgccaaa gtaaatttgg gttacaaaaa
    42961 cggcgatgag gtttgcgtgc gctcggacta taccggttac gttacctgca acacaggcaa
    43021 cttatccgat aaggctttaa acagctttga tgcgacacgg attaacggca atgtgaattt
    43081 gagtcagaat gcggcattgg tcttgggcaa ggctgcatta tgggggcaaa ttcaagggca
    43141 aggaaacagc cgtgtcagcc taaaccaaca tagcaaatgg catttgaccg gcgacagcca
    43201 agtacacaat ctgtcattgg cggatagcca tattcatttg aacaacgctt ccgatgcgca
    43261 aagtgcaaat aaataccaca cgatcaaaat caatcattta tccggtaacg ggcattttca
    43321 ttatctgacg gacttggcga aaaatcttgg ggataaagtg cttgtgaagg aatccgcatc
    43381 cggccattat cagctccatg ttcaaaataa aacaggcgaa cctaatcagg aagggctgga
    43441 tctctttgat gcatcatccg tacaagaccg ctcccgcctt tttgtttcct tggcaaatca
    43501 ttacgtcgat ttaggcgcat tgcgttatac aatcaaaaca gaaaacggta ttacccggtt
    43561 gtacaatcct tatgccggga accgccgccc agtcaagccg gccccgtctc ctgccgcaaa
    43621 cacggcttct caagcacaaa aggcaacaca aacggacggt gcacaaattg ccaaacctca
    43681 agatattgtc gtcgcaccgc ccagcccgca ggcaaatcaa gccgaagaag caaaacgcca
    43741 acaagcaaaa gcggagcaag tgaagcgtca gcaagcagaa gcagggcgta aatcggcaga
    43801 gttgtcggca aaacaaaggg ccggggaaga ggaacgccgt caaacagccc aatcccagcc
    43861 gcaacgccgc aaacgccgtg ccgcaccgca ggattatatg gcagtttccc aagaccgtcc
    43921 gaaacgccgc agacaccgtt cggtacagca aaacaatgtg gaaattgccc aagcccaagc
    43981 agaacttgcg cgccgacagc aggaagaacg caaagcggca gagttgttgg caaaacaaag
    44041 ggcagaagcc gaacgtgagg ctcaagcatt ggctgcgcgc cgaaaagccg aagccgaaga
    44101 ggcaaaacac caagctgccg aacttgcaca tcgccaggaa gcaaaacgca aagcggcaga
    44161 atccgcgaaa cgaaaagctg aggaagaaga gcaccgtcaa acagcccaat cccagccgca
    44221 acgccgcaaa cgccgtgccg caccgcagga ttatatggca gtttcccaag accgtccgaa
    44281 acgtcgcgga cgcagatcta ctctgccggc accgccctcg ccatcatttg attcatcagc
    44341 ttacgcagca cccagggcct tgcataatcc ggactggtat gaaaatgatt atgaagaaat
    44401 ccccttggat gcgttggaag atgaagatgt atccgaatcg gttgacacat cagacaaaca
    44461 gcctcaagac aatacggaac ttcatgaaaa agttgaggca gtgagtttgc aaccaagagc
    44521 cgcgcagccg cgaacccaag ccgccgcgca agccgatgca gtcagcacca atactaactc
    44581 ggctttatct gacgcaatgg caagcacgca atctatcttg ttggatacag gtgcttcatt
    44641 aacacggcac attgcacaaa aatcacgcgc tgatgccgaa aaaaacagtg tttggatgtc
    44701 aaacaccggt tatggccgtg attatgcttc cgcacaatat cgccggttta gttcgaaacg
    44761 cacgcaaaca caaatcggca ttgaccgcag cttgtccgaa aatatgcaga taggcggagt
    44821 attgacttac tctgacagtc agcatacttt tgatcaggcg ggcggcaaaa atacttttgt
    44881 gcaagccaac ctttatggta agtattattt aaatgatgct tggtatgtgg ccggcgatat
    44941 tggtgcgggc agcttgagaa gccggttaca aacgcagcaa aaagcaaact ttaaccgaac
    45001 aagcatccaa accggcctta ctttgggcaa tacgctgaaa atcaatcaat tcgagattgt
    45061 ccctagtgcg ggtatccgtt acagccgcct gtcatctgca gattacaagt tgggtgacga
    45121 cagtgttaaa gtaagttcta tggcagtgaa aacactaacg gccggactgg attttgctta
    45181 tcggtttaaa gtcggcaacc ttaccgtaaa acccttgtta tctgcagctt actttgccaa
    45241 ttatggcaaa ggcggcgtga atgtgggcgg taaatccttc gcctataaag cagataatca
    45301 acagcaatat tcagcaggcg ccgcgttact gtaccgtaat gttacattaa acgtaaatgg
    45361 cagtattaca aaaggcaaac aattggaaaa acaaaaatcc ggacaaatta aaatacagat
    45421 tcgtttctaa aataccaaat tcatagcaaa ataaaatgcc gtctgaactc aagcttcgga
    45481 cggcattttt atcagactaa caaagctaca gctcaatgcc tttgagtttc gccacggtat
    45541 tgatgtcttt gtcgccgcga cccgacaggt tgaccaaaat cacttggtct ttacccattt
    45601 tcggcgcgtt tttcaccgcc caagcaacgg cgtggctgga ttcgagcgcg gggatgatgc
    45661 cctcgaatcg gcagagcaag tcaaaggctt cgagtgcttc gtcatctttg gcaacggtgt
    45721 attcgacgcg cttgatgtcg tgcagatggc tgtgttccgg gccgatgccg gggtaatcca
    45781 agcctgcgga aacagagtgc gtccccaaga cttggccgtt ttcgtcctgc atcagatagc
    45841 tgcggaagcc gtgcagtacg ccaatcggtg cgcccgaagt aatcggcgcg gcgtggtcag
    45901 gcgtatccac gcccaaaccg cctgcttcta cgccgaccaa acgtacgttt tcttcttcga
    45961 tatagggata gaacaaaccg atggcgttcg agccgccgcc gacgcaggca acggcgacgt
    46021 cgggctgtct gccgatagct tcctgcatct gcgctttggc ttcgttgccg atgacgcatt
    46081 ggaaatcacg caccatttcg ggatacggcg cggggccggc ggcggtgccg atgatgtaga
    46141 acgtatcgtc cactcgggcg acccattcgc gcatcgcttc gttcatcgcg tctttcaaag
    46201 tacggctgcc actgtcaacg ccgatgacat tcgcaccaag cagcttcata cggaacacat
    46261 tcggcatttg gcgttgaata tcgtccgcac ccatgtacac gtcgcaagtc atgccgaaac
    46321 gtgcggcgac ggtggcagaa gccacgccgt gctgacccgc gccggtttcg gcgatgacgc
    46381 gttttttgcc catacggcgg gcaagcaatg cctgaccgat ggtgttgttg accttgtgcg
    46441 cgccggtgtg gttcaagtct tcgcgcttca accagatttg cgcgccgccc aaatgctcgg
    46501 acaaccgcgc ggcgtggtaa acggggctgg gcctgccgac ataatgtttc aaatcgcggc
    46561 ggaactccgc ccaaaattca ggatcgtttt tcgcttcgtt gtaggcctgt tccagctctt
    46621 tcaaagcggg aatcagggtt tcggaaacat aaagcccgcc gtgttcgccg aaaaagccct
    46681 tttcgtcggg cgcgtggtag ttcttcatcg gattcttcct tatgcttcag gttttcagac
    46741 ggcatttccg ttttttcaaa catgccgtct gaacgggaaa ttgtcggcaa ttataactgc
    46801 ttttcacaca agcccgccat ttcgaaaaac gcccgatacg ccgctgcctt tttcgccaaa
    46861 ctcaaaggat aggcgcgcga agtcgaaggc aggcgcgtca gtgtcaaatc ccgtccggca
    46921 aacggaaacg gcacggtttc gcccgttttc ggcattttga tgccgccgcc ctgaatatcg
    46981 agcaggattt ccgtcgcctt gccgcctgtc gtacaaatat ggcggcagtc tggtattttc
    47041 gccaacaccg ccgccaaatc gacggtttca actaccttta aaaacttgtc ggacgcattg
    47101 ccgtgttccc ttgccgcctt caacacggtc ggacaggacg caatcccccg ttcgtgcaaa
    47161 aacgccttga ttttctccgc gtcaaacgct ttttcagagt gcgcagcctc cccgtgggac
    47221 ggcatccgga aatgcgccgc atcgttaaaa aacaccagcc cgtaaacgcg ccacatatcg
    47281 ttttggaaat tcggataatg aaactgcatc gcgcgtttgt cttccttggg cggaaacgtc
    47341 cccatcatca tgaccgcagc cttcggcggc aacacgggat cgaagggatg ggtttcgatt
    47401 tcaagcagat tttcagacac gttcaatcct ccaacaggca aagcggcaga cagccggttt
    47461 gcaccaaacc gccaaacacg gcaagccttc aaacagcatt atcacgccct gccgttttcg
    47521 gttacaatgc cgacatcaaa cggatactgt aaacacattt atgttccaac acacaggacg
    47581 acacataaag caccgcccta tgtgtcgtcc tgatttggaa ggggttacgc cccgcccaaa
    47641 cagagtctga tcctgccgcc ctaaagggcg gggtttcaac cgaagaggaa acacgatgaa
    47701 agaacacaaa gcccgcaagc gtttcgggca gaattttttg caggacacgc ggattatcgg
    47761 cgatattgtc aacgccgtgc gcccgcaggc ggatgatgtc gtgattgaaa tcggcccggg
    47821 tttggcggca attaccgaac ctttggcgaa aaagctgaac cgcctgcacg ttgtcgaaat
    47881 cgaccgcgac atcgtatgcc gtctgaaaac gctgccgttt gcggataaac tggtgattca
    47941 cgaaggcgac gtgttgcagt ttgatttcaa cggcatctca ggcaaaaaga aaatcgtcgg
    48001 caacctgccg tataacatct ccacgccgct tttgttcaaa ctggcggagg tggcggacga
    48061 tgtcgccgat atgcacttta tgttgcaaaa agaagtggtc gagcgcatgg ttgccgcgcc
    48121 gaaaagcaac gactacggtc gcttgggtgt gatgctgcaa tattttttcg atatggagct
    48181 gctgattgac gtgccgcccg aatcgttcga tcccgcgccg aaaatcgatt ccgcagtggt
    48241 gcgcatgatt ccggtgaaac accgtatcgg caaggcggac gatttcgagc atttcgccaa
    48301 actcgtgaaa ctcgccttcc gccaacgccg caaaaccata cgcaacaatc tgaaagagct
    48361 tgccgacgac gatgatttgc aggcagtcgg catcagtccg caagaccgtg ccgaacacat
    48421 cgcgccggag aaatatgtgg cgttgagcaa ttatctggcg gacaaggccg tctgaaaagg
    48481 aacgcggcga tggaaagcaa cctgcagaaa accgagcgtt tgatccgcga aatcaaccgc
    48541 ctgcacgcac aatattcgca ggattatttt gaaaccggca aagtgcggaa aatcaatttg
    48601 agccacacgc tcaaaaacgt tccgaccgaa catattttgt cttaccgcct taatttgcac
    48661 gaagcagtta acgattattt agcctttgcc gatacgcgcg gcatagactt tttctaccgc
    48721 gtgaaaaccg cagaaagcat acatgataaa atcaaccgct atctggcacg cggcacccaa
    48781 tatccgacca acaacatcct gaacgatatt ttcggcgcgc ggctgatttg gccgtctgaa
    48841 acagtcgccg ggattttaga aaaacttgac ggctggaaaa cagaatacgg cttgaaaaac
    48901 tggtatttgc gcgatgccgg cggctatatc ggcatacaca tttatttcaa aaacagcagc
    48961 aatttctact atccgtggga attgcaggtt tgggatgaaa aagatgccaa agccaatatt
    49021 gaaaaccata tggcgtacaa acggaatttc gtgcgttgaa ttgccggcaa gccgccttga
    49081 aaccgtttat aactgtttat aagagaataa accataaaat acacaaacgt attcctgact
    49141 tttcctttaa ccgcccgagt gaacaaacta tgaaatacac agccgcactc ctgacttttc
    49201 ttttaactgc ctgcatgaac ccgaacgatg cgtttttcca aaaccggcgt tatcaaatgc
    49261 cggaggcgca attgaacggt tcaaacgccg ttttccatta cggttattcc caaaatcccg
    49321 atcatgattt attggtcgac caagtccgcg tgccggacgc gtacagggct tcatttgcgc
    49381 gggattggat agcgaaggag caaggttacc gcaaaaacga ctgcgtactg gaaaaagcgg
    49441 aaagcggggg tgtgacgtac tacacctgcg atgcggaaaa aggattcaac ggcacacgtt
    49501 tcgtcatcta tgtcgtgaat atgaaaaacg acaaaggcta cgtcaaagtt taccggggca
    49561 tgaataagcc gaatcaggaa gagttaacca aattggttgc cgatttgaat aagttttacc
    49621 cttaatcagc cagacggatt cagactgccc gaaaagaatg gacactatga tcaaattcaa
    49681 aaacgtacac aaacatttca aagacctgca cgtcatcaac ggcgtaaact tggaaatcaa
    49741 aaaaggcgaa gtggtcgtcg tctgcgggcc ttcgggcagc ggcaaatcca cactcatccg
    49801 caccgtcaac cagcttgaaa gcatcgaaag cggcgagatt tgggtggacg gcgtcaacgt
    49861 tgccgacccc aaaaccgatt tgaacaaaat ccgcgaagaa gtcggctttg tgtttcaaag
    49921 tttcaacctc tacccgcacc tgaccgtgtt ggacaacatc accctcgcac cgatgaaggt
    49981 taaggggcaa aatgcggaac aggcggagaa aaaggcaatg gagcttttgg aacgcgtcgg
    50041 actggcacac aaaaaagacg ccttcccttc ccaactttcc ggcggccagc agcaacgcgt
    50101 ggcgattgcg cgcgggctgg cgatggagcc gcgcgtgatg ctgtttgacg agccgacttc
    50161 cgcactcgac cccgaaatgg tcggcgaagt gttgaaagtg atgaaagact tggcggaaag
    50221 cggcatgacg atgatgtgcg taacccacga aatggggttt gcccgcgaag ttgccgaccg
    50281 cgtgattttc gtcgataaag ggcaaatcct tgaagacgaa acgccggaag cgtttttcac
    50341 caacccgaaa cacgaacgcg ccaagcagtt cctgcaacaa gtcatgaccc attgatgcta
    50401 tgccgtctga aaaccgaacg ttcagacggc atagcccgac accgcaagat gattgagaaa
    50461 gcacaccatg tattttgttg accgcaccgc cgccgtcctc aagccgaccg cgcgcttttt
    50521 ggaatggctc aaaagcaccg acgaaaatat gcccgacctg accatagaac agcttcgcgc
    50581 cgactgttcc gtcttcctcg ttccgcagtt cgacgaaccg gaagccgtcg tttcctactt
    50641 cgacgaacgc taccggcaga tttttgaggc ggagctggca ggttgggaca tcgacaagga
    50701 caaatggccg caagatatgg ggctgaaggc gttttgggaa tttttcgaca ttgaaatcca
    50761 cgatatggtt ttggatatgg aagaagcgga tttgaacatc acgcccgtgt tcgacaatat
    50821 gatgtaacgc catgcgcgcc tttcagacgg cattgaggcc gtcccgcatc ctgaacatcc
    50881 tgaccgtatc actccactcg gcttccattg ccgtctgcct gacttggttt tacggccgga
    50941 tgatgtggtt cggtttggcg gctttggcgg caagttacgc atactcgctc aggataacga
    51001 atctgaaaca ccgccatgcc ataaccgcca ttaccataga ccgcgacgga cgggcggaaa
    51061 tcgtatccgg caaagacaaa acagcggcaa cacttgcggg cagcagtatg gttacgcctt
    51121 atgccttatt cttacaatgg gacacaggcg gcaaaaccgt ccgccaatgc atcatgcccg
    51181 acatgacgga caaagaatcc taccggcggc tgaaagtctg ggtgctttgg cggcagccga
    51241 aaaaaaccgc cgaaaccgat acatcagact gatttcagac ggcatttgaa aacgaaacac
    51301 tcatgaaaac attacaagac tggctctcgc atttggaaac cgcccacagc ggcggtttga
    51361 tcgacatggg tttggagcgc gtaagcgaag taaaaaagcg catgaacctg acaccgcaat
    51421 gccccgtcgt cgtcgtcgcg ggaacaaacg gcaaaggttc ggtctgcgcc tacctgacac
    51481 agatttacaa acaggcaggc ttcaaaaccg gcacgctgac cagcccgcat ttattgcatt
    51541 acaacgaacg catcgccatc aacgccgaac ccgtttcgga cgatacgatt attgcctctt
    51601 tcgagcgcat cgaagccgcg cgcggcgaaa tatcgctgac ttattttgaa ttcaatgcct
    51661 tggcggctgt cgacattttc atacgcgaac aagtggatgt aatgatattg gaagtcggct
    51721 tgggcggacg tttggacgcg gtcaacgcgt ttgacggcga ttgcgcggtg gttactagtg
    51781 tggatttgga ccatcaggca tttttgggcg atacggtcga acaggtcggc tttgaaaagg
    51841 caggcgtgtt ccgcagcggc aaacccgcca tctgcggtca aaaccccgcg cccgcatcat
    51901 tggtcgcaca cgccgaagcc ataggcgcga aactgctgat ggtacagcgc gatttcgagt
    51961 ttcacgcgat ggaaaacatc caatggaact accgcttccg gccgcagcat tcagacggtc
    52021 ccgcacgcaa ccgcaatgcc ctgcccttcc ccgcattgcg cggcgcatac cagctttcta
    52081 acgccgcctg cgcgttgacc gtattggaat gcctcgacga cagactgccg gtggacattg
    52141 gtgcaatcaa acgcgggctg ctgctggttg aaaatcccgg acgcttccaa gtcctgcccg
    52201 gccggccgct gaccgtattg gatgtcggac acaaccccca cgccgcccgc gccctgcgcc
    52261 gcaacctaat caaccttgcc tacgcgcaaa agcgcaccgc cgtgttcagt atgctgtccg
    52321 acaaagacat agacggcgtg tcggaaaccg ttaaagacca gtttgacgag tggtatatcg
    52381 cgcctttgga cgtgccgcgc ggtatgacgg cggatgcact gaaagccaaa ttggaacaac
    52441 accacatcga aaacatacag acattcgccg ccgtccgcga cgcgtaccgg gcggcagcct
    52501 caaaagcggg cgaagacgac agaatcgtcg tattcggctc atttcatacc gtcgccgacg
    52561 taatgtcggt actgtgaaaa ggaaacttct atgtccgaaa acaaacaaaa cgaagtcctg
    52621 accggttacg aacagctgaa acggcgcaac cgccgccgcc tcgtaacggc aagctccctg
    52681 gttgccgcct cctgcatcct gctggcagcc gcactcagtt ccgatcctgc cgacagcaat
    52741 cccgcaccgc aggccggcga aaccggcgca acggaaagcc aaacggcaaa cacggcacaa
    52801 acccctgcct tgaaatccgc cgccgaaaac ggggaaaccg ccgccgacaa accgcaggac
    52861 ttggcaggcg aagacaagcc ttctgccgcc gacagcgaaa tcagcgagcc tgaaaacgta
    52921 ggcgcgccgc tggtgctgat taacgaccgg ctcgaagaca gcaacatcaa aggtttggaa
    52981 gaatccgaga aactgcaaca ggcagaaacc gccaaaaccg aaccgaagca ggcaaaacaa
    53041 cgcgctgccg aaaaagtgtc ggcaactgcc gacagtacgg atacggtagc ggttgaaaaa
    53101 ccgaaacgca ctgccgaacc caaaccgcaa aaagcggaac gcactgccga agccaagccc
    53161 aaagccaaag aaaccaaaac cgccgaaaaa gttgccgaca aaccgaaaac tgctgccgaa
    53221 aaaaccaaac cggatacggc aaaatccgac agcgcggtaa aagaagcgaa aaaagccgac
    53281 aaggctgaag gcaaaaagac agccgaaaaa gaccgttcgg acggcaaaaa acacgaaacg
    53341 gcgcaaaaaa ccgacaaagc ggacaaaacc aaaaccgccg agaaggaaaa atccggcaag
    53401 gcgggcaaaa aagccgccat tcaggcaggt tatgccgaaa aagaacgcgc cttgagcctc
    53461 cagcgcaaaa tgaaggcggc gggtatcgat tcgaccatca ccgaaatcat gaccgacaac
    53521 ggcaaagttt accgcgtcaa atcaagcaac tataaaaacg caagggatgc cgaacgcgat
    53581 ttgaacaaac tgcgcgtgca cggcatcgcc ggccaggtaa cgaatgaata gcctccccat
    53641 tgccgacctc ctcgcctccg ccgtcatcgc cgcctgcatc gtcatttcca cgatgcgcgg
    53701 cgtgattgcg gaagcaggtt cgatggtggc atgggtggtt tccttctttt ttgccaaact
    53761 ctttgccgca cccttcgccg acctcgcctt tgcctcgttc caaccccgcc tgtttgcatt
    53821 ggctctgtca ttcatttccc tgttcgtcat tgcctgtctg atccagaaaa tgctccgttc
    53881 gctgctgacc ggcgcagttt cggcggtcgg tctgggcttt gccaaccgca ttttgggcgg
    53941 tgtattcggt gcattgaaag gcgttttgat tgttaccctg ctgatcatgc ttgcttcaaa
    54001 aaccgacctg cccgataccg aagaatggca acagtcctat accgtaccgt ttttcgtatc
    54061 gctttccgaa gcggtgttaa accatacgga caacgcaccc gaatccctcg acgacgacta
    54121 atccgcatac gtggcgtttc agacagcata cctgatttat gccgtccgaa acgccttttc
    54181 tttccttgac acaccccgac agacaaagcg cacctgcctg actttacaca aatattgtca
    54241 acaattcggg caaaaagccg ccgtttgctt tataattccg tttcaccctt aaacctcgaa
    54301 tcggagaaga cgatatgtgc ggcgtattag gtttggtcag tcatgaaccc gtgaaccagc
    54361 ttctgtacga cggtttgcag atgttgcagc acagggggca ggatgcggcg ggcattgcaa
    54421 cggcggaagg cggtaccttc catatgcaca aaggcaaagg gatggtgagc gaagtgttcc
    54481 gcacgcgcaa tatgcgcgat ttgaccggca acgccggcat cgcccacgtc cgttatccca
    54541 cggcgggcaa cgccggcagc agcgcggagg cgcagccttt ctacgtcagc tcgcctttcg
    54601 gcatcgtttt ggcgcacaac ggcaacctca ccaacactgc cgaactgtat gaaaacgtgt
    54661 gtaacaaaca cctgcgccac atcaacaccg gctccgattc cgaagttttg ctcaacgtat
    54721 tcgcgcacga attgcgccgc gaagtctcta aaaacgccga cccgcaccgg ctcaatgccg
    54781 acaatatttt caacgccgtt gccgaagtcc accgcctggt gcgcggtgca tacggcgtgg
    54841 ttgccatgat tgcgggctac ggtatgctcg ccttccgtga tccttacggc atccgcccgc
    54901 tggcattggg ttcgcaaacc gacagcgaag gcaggaaatc ctatgccgtc gcctccgaat
    54961 ccgtcgcctt caacgcgctt gcctacgatt tggaacgcga tatccggccg ggcgaagcgg
    55021 tatttgtcgg ctttaacggc acaatcatcg cccgacaatg cagcgaccgc gccaaactca
    55081 gcccctgcct ttttgaatat gtttattttg cccgccccga ctccgtgatt gacggcgtgt
    55141 ccgtttacca gtcgcgcttg gatatgggcg tgtccttggc ggaaaaaatc aaacgcgagc
    55201 tgcccgtgga cggcatcgac gtcgtgatgc ccattcccga taccagccgc cccagtgcaa
    55261 tggagcttgc cgtccacctc aacaaaccct accgcgaggg tttgattaaa aaccgctata
    55321 tcggccgcac ctttatcatg cccggtcagt cgacgcgcaa aaaatccgtg cgccaaaaac
    55381 tcagcccgat ggaaaccgaa tttgcaggca aaagcgtgtt gctggtggac gactccatcg
    55441 tgcgcgggac gaccagccgc gaaatcgtcg aaatggtacg cgcggcgggc gcgcgcaaag
    55501 tctatatcgc ctccgccgcg cccgaagtgc gctatcccaa tgtgtacggc atcgatatgc
    55561 ccacgcgcga agagttgatt gccaacgggc gcagcgcggc ggaaatcgcc gccgaaatcg
    55621 gcgcggacgg catcgtgttt caagacttgg gcgatttgga agccgtcgtc aaagcactca
    55681 acccgaaaat cgaatccttc gattcatcct gtttcaacgg catttatcag accggcgaca
    55741 tcgatgatgc ctacctcgac cgcctgtctg ccgagaaatc cggctgcggc ggtctgaaaa
    55801 tccacccgag caggatggaa cacagcatca gcatcagcga tgcgggcgac gaagaataag
    55861 gcttcccaaa ccgcgatgcc gtctgaaatc cgaacgttgg gttttcagac ggcatttccc
    55921 atatcggcat cacatttccc gtttcatccg cacacgatat gagtaccgaa accaaaaact
    55981 acattacgcc cgccggctgg caggcattga aagacgagct ttatcaactg gtcaataaag
    56041 aacgccccga aatcgtccaa atcgtcaact gggcggcagg caacggcgac cgcagcgaaa
    56101 acggcgacta tctttacggc aaacgccgta tgcgcgaaat cgaccgccgc atccgtttcc
    56161 tgaccaaacg tttggaggct gccgtcgtcg tcgatcccga attgcgcgaa gcgaccgacc
    56221 aagtattttt cggcgcgacc gtcggtctgt tgcgcggcga cgggcgcgag caaaccgtca
    56281 aaatcgtcgg cgtcgatgaa atcgataccg cgcaaaacaa aatttcttgg atttcgccgt
    56341 tggcgcgttg cctgattaaa gcgcgcgaag gcgacgaagt ggttttgaac acgccggaag
    56401 gccgtgagga aattgaaatc ctttctgtcg aatacatcaa aatcgactga tcgggaatgc
    56461 cgtctgaaac ggcacaagcg caccgtttcc caaataaagg aataccgaat gaggaaaatc
    56521 cgcaccaaaa tctgcggcat caccacaccg gaagacgcac tgtatgccgc ccacgccggc
    56581 gcagacgcat tgggactggt tttttacccc caaagccccc gcgctatcga catcattaaa
    56641 gcacaaaaaa tcgccgccgc actgccgccg tttgtcagcg ttgtcgccct tttcatcaac
    56701 gaaagcgcgc aaaacatccg ccgcatcctt gccgaagtgc cgatacacat catccaattc
    56761 cacggcgacg aagacgatgc attctgccgg cagttcgacc gcccctatat taaagccatt
    56821 cgtgttcaga cggcatcaga catccgaaac gccgccacgc gcttccccaa cgctcaggca
    56881 ctgctgttcg atgcctatca cccttcggaa tacggcggca ccggacaccg cttcgactgg
    56941 acgctgttgg cggaatattc gggcaagccg tgggtgcttg ccggcgggct gacccctgaa
    57001 aacgtcggcg aagccgtccg catcaccgga gcggaagcgg tcgacgtatc cggcggcgtg
    57061 gaagcgtcta aaggcaaaaa agaccccgcc aaagtcgccg cctttatcgc aaccgccaac
    57121 cgcctatccc gttaaagcaa caaaaattgc cgccggaatg acttatagtg gattaacaaa
    57181 aaccagtacg gcgttgcctc gccttgccgt actatttgta ctgtctgcgg ctcgccgcct
    57241 tgtcctgatt tttgttaatc cactataatc tcaaaaattt atgccattaa atcaataatt
    57301 tctgatgaat tttgaaaact taatcccgtc attcccgcgc aggcgggaac ccggttcatt
    57361 gagcttcagc tatttagaat aaattttgaa actctaatcg cgtcattccc acgaaagtgg
    57421 gaatccggtt ttttgagttt cagtcatttc cgataaattg ccttagcatt gaatgtccgg
    57481 attcccgcct gcgcgggaat gacggcggaa atcttgttta tattgaatca aaaaaacctg
    57541 caccttaatc agttggcggt ttagtccgac ttttggggtg cagatcaagc ttccagacgg
    57601 catttccttt aaaacttcat ttcgagcgcg aggctgaagt tcctgcccgg tgcggcatac
    57661 cttccatagt tgctgtcgcc gccgtgccgg tttgccgtgc tttccgcagt ctggcgcaag
    57721 gattcccaag taacgtagcg gtagttgccg atattgtaga tagccgccct caaggtcagc
    57781 cgttttttca gattcagata agcggaaaca tctgccgttt tccaagaaga cgtaaccctt
    57841 cctgccgaat atcgtttttg atcgcctgcc agataagcaa gctcgtcagg gtttttccct
    57901 ttggaatagg tcagcataat gtttgcgccc catttcccct cgggctggtc gtatccgaac
    57961 cccaaaacat aacgcgacgg ctgtaccgca tccaaagcat agctgcggag ggacaagtcc
    58021 ggccggttgg ataccgattt cggtttgata cggttgtacg ccaatgtggt gtacaggcct
    58081 tcgggcagtt tgccgtatac gccgttccag tcgattttcc ccaagatgtt gatgccttga
    58141 agcgacatat tttgggcatt gtaataatcg cgtatatcaa tctctgtcaa tcgtcctgct
    58201 gaatccggca atttggtttt ttgatcggca acggcaatca tatcggtata acggttgcgg
    58261 aaactgctga tttccaaaaa gccgaaatcg cccttccact gcaaaccgat ttcccggttg
    58321 gctgcctttt ccgatttcag ggcgggacgc tgccagcctt tcggataatc gtgataaatg
    58381 tctatcccga aaagttcttg gaaggaaggc gttctgaagc cgctggaggc gcggtaagac
    58441 acggaaaaat gccggttcgg tttgaacacg atgccgctgt tccacgaacg gtcggcatac
    58501 cgcccgctgc ggacgagttc ttccgacgtg gtgaagtttt tccggtcgta cctgccgccc
    58561 aagctgaaat caaaatattt gccgattgaa aaacggtcgt tcaaagaaat gtggatattg
    58621 ctgccgttga tttttcttgg cacgcatttg cgggaacgca gggtttcgat atagccgcag
    58681 accgaccctt cgacgacttc gggcttaccc aaacggtact tatcttgatt cttttcatcg
    58741 aatcccgtgg attccgaaat ccttgccgca ttgtgggaaa gctgttctgg gcgggagatc
    58801 gctttggaag catcgtaacc gaagcccaaa gtcagatggt gtttcgtcca tttgtttttc
    58861 agcgatttct caaacgaggc attcaaaaca ttgtgctgtt cgcggtagtg gaaacggtcg
    58921 ctgctgtcgt aggaatacgg tttgtccgcc gacgcgcggc aggatttgtc cacggcagga
    58981 tacacggcgc aattcagctt cagcgtgttg ttgtcggttg ccacgccctg tttgtcaaac
    59041 gacaacaccg ccttatccgc ccagttgtcc gagtatttct cattttcata acggtacagc
    59101 aaacccatac ggcggcggcg gtgatgttcg tcaataaatt tggtgcggga atatttcaaa
    59161 cctatgccct tgaccaaatt tttatcgccc ttccactctt ctatattcgg cacaaaatac
    59221 aagccgtcgc ggaaatcgtc gccgtcgtac accccgctct tgtctctaaa cttttccgcc
    59281 tcgtccgtac cgtaatactg tttttccgtc atatcgcgga tgtcgtaacg ctgtttggta
    59341 tcctcaaaca cgccgccgac ataatgcctg ccgccgaagc ggtagcccag cttggcaagc
    59401 caagagccgc tgcggtaatc catcggatcg ggcaatatcc tgccgccgcc cgtgtaggct
    59461 tgggcggaca ggttttcgtg gcgcgcctgc gcctcccgca cctgcgcctc ttcttcagca
    59521 cttaaaggct gattttgttc aatacgttct tttacccagc ggttgagctg gcggttcaaa
    59581 tatatcccgt agcccgccaa ttttgccacg ggcttgagtg tattctcgcc ctctactaag
    59641 aaaaatggct ctgttgtctt gcgtttaata tcgtatgtct ggcggaacgc gtccaaacgg
    59701 cctatgccgt attccacccc gtccgcaata tcgccgtgcg ggcgcgtttc ccgcccttgg
    59761 cgttcggttc ggattagcag cccttcccaa ccgtctttgc tgaaccccgc gccgagcgac
    59821 ttcataaatt ggcggttttt actgccgtaa gcggtttttg cctgtatccc ccggcttttg
    59881 ccgtctgaaa tcaggtctgc cgcctctttg gtgcggaagg cgaccgcgcc gccgagtgcg
    59941 ccgctgccgt gatcggacga accggcgcct ttgtcgattt ccaccgtgct gatgttttca
    60001 tattcgattt cgttgattgc gccgctgccg ccgcgtccgc cgtatccgct caacgatccc
    60061 tgcacggtaa acgcctgtat ttgggcaacg ccgtcaaccg aaaccgccac acggttttta
    60121 tctacgccgc gtatcgagta gccgccgctc gcgccgttgc cctgttcgac aaccgccacg
    60181 cccggatcgt agcgcgtcag gtcgcggata ccgagtacct gttctttgtt caacgtttcc
    60241 gacgttttga cgattttgcc caaacctgtc gcctctttcg atcgccgtcc cactttggcg
    60301 gcacggacgg taacctcttt caatgattga ctttgcgccg catcaagtgc cgccgccccc
    60361 gcttgggcag cataagccgg aaaagcggtt gcaatggcca acgccgtcaa agtcagcgga
    60421 aaaccgtgtt tcttattcat ttttccacct ctttattatc tttcttcgca ccgaatacca
    60481 cgccgaattg gggtttaact tcaggttcta actgttcgcc aacatcagct tcattttcaa
    60541 cttcattttc aatatcttca gttataccaa gagatttccc atccttattg aaaatagtgc
    60601 cgcccaattc cgccgcctgc gggccgtaaa agccgcccgt tacaagaaga ttgtcggctt
    60661 tgaaactttg aggattagtc gaaccatttc ccgaaagatt gatgccgtta tcccgagtgc
    60721 gtgctgttgc gtggaaaccg ttgccctcaa tcacgccgtt ttcaatacgg aaagcaggtt
    60781 ctacaccgtt ttgctccgtc agcgttccgg aaatcgattt attgccgaaa tcaacgtcaa
    60841 attctgcttt tgccgcttta tccgccttat tatcccattg aatgggttcg ccgatacgcg
    60901 cttcccaagt gccggtataa tgcgccgttc cggtttttgg aatgtcggct tccgccgtgc
    60961 ggataccttt caggaaaagg tcgatgtccc tgcctttaag ggcttccggg gcgggcggga
    61021 tgccgtctga aacaccgttg ccttcttccg gcgattcttc ttcgggttct tcctcttcag
    61081 cttcttcttc gatttcttca gcttcatcag cttcttcttc gacgatttcg tcttcgtctt
    61141 cgccgttatc atcttcggaa acttcgtctt cgctttcttc ggcgatttct tcgtcttcgc
    61201 tttcttcacc gttattaata cccgaatcct cttcgtcctg cgccttcggt ttgacggcgg
    61261 ggcgttcggt ttttatccgt ccgagtttca cataggtcaa aaagtcgcaa caagcactga
    61321 cggtcatttt cctgccgtcg gcaagatcga tggttttctc tttgctaacc aaaggaattt
    61381 cacgcccttc gacaagaagt ttgtcaggat gaccaaaatc gggcatagtg gaaacctcaa
    61441 acggacgggg attttcatca gtcgcctcgt caacggaaat ttttagagaa tccaagattt
    61501 tggtgtgttt tccaaacggc atagcaggat ttgtgcctga tgcgtttgtt ttttgtttgc
    61561 ctgcgaatac gccgaatacg ccgttgtcgt tactgataaa ccgtccggca agctcttctc
    61621 cgttatcgcc gaaaaaaccg ccctcaagcc gctgatcggc atcggtatgg aaaaacaaat
    61681 attctttatc agcgtgtctc gtcttcacct cggtgttaac tttggcactg ccggtaaagc
    61741 ggttgccgtt taaatcggca gtgatgttgt aaatggtcag cggtttttta ggttcctttg
    61801 gatcatttct cttttgcaca tactgatttt taatcagctc gccctttagg atttttttgt
    61861 cgaaatcaac cgtatattcg gcaggatgtt tttccctgtc gtcggcatcc ttagccgcat
    61921 aagaagttgc accaatatcg ttaccataaa atgttatata acccaaatcg tcactggcaa
    61981 ccgccttacc tgtccgatga cgtttggcat cggtcatata ttgccagttg ccgaaatatt
    62041 ttaccgttcc cgcgctcggt aaagattggg aaggacgttc tccggaataa tatacaaaac
    62101 cgtcataacc aaaacggtgg gtaaaccgct taccacctga gttctgttcg atttcatctt
    62161 ttccgttttt agtatataca taaccggcat ccacaaattt ataattatat tttttatttt
    62221 ccttttctga cgtcctaatt tctacatcgg aatcacgttt atgaatttcg cttttaagtt
    62281 gttgaagttt atttcctttt gaaccgtata aaaacagaac atcaccttct ttaaacgaca
    62341 gcttttcttc tgcttgtttg ctatcgggaa ttgccgtgcc atcttcacga tgaaaagcag
    62401 tattccgcct caacaaccgc attgccgcac cgacggcggg ccggttgacc ggcgtggttt
    62461 ctaccgaagg ctcggcaggg ggggggaagt gggaacgtcc ttagacttga aagtgacggg
    62521 gtacgcggtc ggcgttgatt cgacaacagg ctgcacgccg aaattgccgc cgcaggatgc
    62581 cagcatcaac ggcagcaagg caataccgta ataattcagt ttacgcatcc ctatcccccc
    62641 tatttaatta ataataatta tcattatatt aatatgcaca gataatatca acccgttttt
    62701 aacaaagata ttcccgattg aaacaaaatg ccgtccgaaa cctgttcaga cggcatttgc
    62761 gtaaagttca gcaagaaaaa ctacaaaccc agctcgcgca ggaagcgaat gtcgtctgcc
    62821 caaccggatt tgactttgac ccagaccttc aaaaatactt tgttatcaaa cagtttttcc
    62881 atatccagcc gcgcttcggt ggaaattttt ttcaaacgct ccccgccttt accgatcaaa
    62941 atcgcctttt ggctttcttt gtcgaccaaa acggcgatgt agatgcggtt caaaccgtct
    63001 ccctcttcaa actgctccac ttcgacgttc atcgcataag gcagctcctc gcccaaatag
    63061 cggaagagtt tttcacgcac gatttccatc gccaaaaaac gcgccgattt gtccgtaacc
    63121 atgtcttcgg gatacatcgg tacgctttcg ggcagatacg gcttgagcag ctccaacagg
    63181 ttggcaatcc gcaaaccgtg tttcgcactg accgcctccg ccgccgcaaa ttcaaattcg
    63241 gcgcgcactt gggcaacaaa cgcctccagc gcgtaacggt ctttcgcctt gtccttgtcg
    63301 attttgttga tcactaaaat gaccggcgtg tgcttgggca gttgtttcaa cacgacgcgg
    63361 tcggcatcgg taaggcgcat cgcctccacg acgaaaacca ccacatccac accgccgagc
    63421 gcctcggtaa cattttgatt cagcctgtcg ttgagcgcgt tgcggtggtc ggtttgaaag
    63481 cccggcgtat cgacaaacac gaactgcgcg gtatcgtcgg tataaatccc cgttacgcgg
    63541 ttgcgcgtcg tctgcgcctt tttgctggta atactgattt tctgaccgat gagatggttc
    63601 atcagcgttg atttgcccac gttcggacga ccgacaatcg ccacgaagcc gcaacggtat
    63661 ccgccggcgg cgcgttcccc tgcaaggaag gtttcaatat ccatattcgc tttcatatcc
    63721 acatttcaga cggcatattc acgcgccgcc tcatttcttt ttcttcttca gcggcagctt
    63781 ctcttccagc catttcaaag cctctttcgc cgcctcctgt tccgccgcct tgcggctcgt
    63841 ccctttggca cggcacacga aacccagttc gcccaaatcg caggaaatga caaacatact
    63901 gtcgttggca tggccgattt gctcttcgat gcggtatttc ggcaaggcga aacggcgcgc
    63961 ctgcaacgcc tcctgcaaag cagttttgcc gtcttttgcc tgattttgga aatcggcgcg
    64021 ccggacgcgt tctgcaaaca aatggcgcac caccttttcc gccgtgttga aatcggcatc
    64081 gaagctgacg gcggcaaaca tcgcctccat cgcgtccgcc agtatcgaag gccgtctgaa
    64141 gccgccgctc ttcaactcgc ccgcccccaa atacagaccg tcgccgacat tcatttccgc
    64201 cgccatttcc gccagcacgc cctcattgac cagactcgcc ctcaaccgcg acaactcgcc
    64261 ctcggtcaac ttcggaaacg cgtcaaacag catccgcgcc accgtataat tcaaaatcga
    64321 atcgccgaca aactcgaacc gttcgttgtg cttcgcatga tggctcctgt gggtcaaagc
    64381 ccgccgcaaa agcgacatat cgcggaacgc gtagcctaac tttttctgta tcgcagtatg
    64441 tgcctgccgt ttcaaaacat cgtctttcat taccgtcttc cgcaaaaaac agcccgaatg
    64501 cgtttccata ctccaaaaat acagaaacac cttaaggctg caaacggcgc acattgtacc
    64561 accaaaccgc cgccgtctgc cgacaacgcg ggaatcgtgg taaaatccgc aagtttcccc
    64621 cccaacgtat tcccgcaatg aacgcatttg cacaagcact ttcctcggca ctcgaccgct
    64681 gcatcgccac caacaccgtc gccaaacaaa accggcccgt cggcttcctt taccgcgaag
    64741 cccccgtttt tgaaaacgac agcggctggc gtttcttcag cggcgacgaa accgacgaat
    64801 acaccgacga ccccgacaac ttcagcatcg tcagcctcgc cgacatcacc aaaaccaacc
    64861 ctgaaaccgc cgcactgctt tcccaacccg aaggcagcgc gtgggagctt gccgaagacg
    64921 gcacattcca aaccgttgcc gactggcagc cgcaagactg aaaccttcag acggcattcc
    64981 gaaaccccga ataccaaagg acacccaatg aacaccatcg cccccaacct cgacggcaaa
    65041 cacctccgca tcggcatcgt acaggcacgc ttcaccaacg aaatcggcag ccaaatgctc
    65101 aaagtctgct gccgcaccct ccaagaattg ggcgtagcag acgaaaacat caccgtcgcc
    65161 accgtacccg gcgcgcttga aatccccatc gcgctgatga actttgcctc ttccgaaaaa
    65221 tttgacgcac tgattgccat cggcgtcgtc atccgtggcg aaacctacca tttcgagctg
    65281 gttgccaacg aatccggcgc agggatcggc cgcgtcgcac tcgactacaa catcccgatt
    65341 gccaacgccg tcctgaccac cgaaaacgac gcgcaggcaa ttgaacggat tggagaaaaa
    65401 gcctcggatg ccgccaaagt cgccgtagaa tgcgccaacc tcgtcaacct tctgctcgaa
    65461 gaacagtttg aagacgaaga ataacaaaca gggcgtacac cgatgcgccc tttccgtttc
    65521 agacggcata ccgtgccgcc caaccgtttc aaggaaaaat catgaaaaca gcccgccgcc
    65581 gttcccgcga gcttgccgta caagccgttt accaatccct tatcaaccgc accgccgcgc
    65641 ccgaaattgc taaaaacatc cgcgaaatgt ccgactttgc caaagcggac gaagaattgt
    65701 tcaacaaact cttcttcggc acacaaacca acgcagcgga ctacatccaa aaaatccgcc
    65761 cgctgctcga cagggacgaa aaagacctca accccatcga acgcgccgtt ttgctgaccg
    65821 cctgccacga gctttccgct atgcccgaaa cgccctaccc cgtcattatc aacgaagcca
    65881 tcgaagttac caaaaccttc ggcggcacgg acgggcacaa attcgtcaac ggcatcctcg
    65941 acaaactcgc cgcccaaatc cgcccagacg aacccaaacg ccgttgatac tcggaaaatc
    66001 cgaatcggaa aatacacaaa tgccgtctga aaacctttca gacggcattt tgcttgaccg
    66061 ttcaatcaat actgcaccgt ccagccgatt tcgccgcccg cgcgcatcgg gacaagttcg
    66121 ccgtcgccgt aagggacgct tgcgggaacg gtttggcttt gtttgacaag ggtaatcgtg
    66181 tcggcgtttt ccggaatgcc gtagaacctt gcgccgtttt ccgaggcgaa ggcttcgagt
    66241 ttgtccaacg cgcctgcttt ttcaaacact tcggcgtaaa gctcgatagc ggtcatcgcg
    66301 ctgaacatac cggcgcagcc gcaggcgttt tctttggcgg atttggcgtg cggcgcggag
    66361 tcggtgccga ggaagaattt gtgcgccttc tcgccggtaa cggcggcgac caatgcctga
    66421 cggtgggttt cgcgcttgag gacgggcagg cagaaatgat gggggcgcac accgccgacc
    66481 aagaggtcgt tgcggttgag caggaggtgt tgcggggtca cggaggcggc aacgttgtcg
    66541 cccgcttcca aaacgaggcg ggcggcttcg gcggtggtga tgtgttcgaa cacgactttg
    66601 agattcggca cttgcgccaa aacgggtttc atcacgcgcc cgataaaggc ggcttcgcgg
    66661 tcgaaaatgt cgatttcggg gtcggttact tcgccgtgaa cgaggaacag gatgccctgt
    66721 tttgccattt cttccaacac ggggatgagc ttgaacaggt cggttacgcc ggaatcggaa
    66781 ttggtggtcg cgcctgcggg gtagagtttg aaggcgacga tgccggcggc tttggcttcg
    66841 cgcacgagtt cgggcgtggc ttggtcggtc aggtaaagcg tcatcagcgg ctcaaacgcg
    66901 ctgccttcgg gcaacgccgc cataatgcgc gctttgtagg caagcgcgtc ggctacgctg
    66961 acgactggcg gtttgaggtt gggcatgatg acggcgcgcc ccatctggcg ggcggtataa
    67021 ggggcaacgg ctttgagcgc gtcgccgtcg cgcaggtgca ggtgcatatc gtcgggtcgg
    67081 atgatggtca gggtttgcat ggttgttcct ttttagattt ttggtttcca gacggcatgc
    67141 cgactttatg ccgtctgaag tcaggggaga attaggggtt tcccgttatg ggaacggtgc
    67201 gttaggttca atactgcctg tacgggtaaa tccggggaat cggcggtttg tgcggtcaat
    67261 acggtttctt cagggctgcc cgattcccgc caggcatagc tgatttgcct ggtttcaaac
    67321 ggtgcctgca ccgactgttc cgccctcaat tctatattca attaaaaaca aaatagtaca
    67381 acactcgacg ttgaaggttt aaccatggca tactctgcgg acttaagaaa caaagcttta
    67441 aaccatagcg gattaacaaa aatcaggaca aggcggcggg ccgcaggcag tacggat
//
LOCUS       DBJGGI010000003        65081 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.003, whole genome shotgun sequence.
ACCESSION   DBJGGI010000003 DBJGGI010000000
VERSION     DBJGGI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 65081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65081
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.003"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            111..335
                     /locus_tag="ACSIGJ_000171"
     CDS             111..335
                     /locus_tag="ACSIGJ_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329165.1"
                     /translation="MMKNKYILIKAAIGITAISIFYLLTQGSIGKTEEPSYFLMFMFL
                     NSLWFEENKTVMAAVTAMIAAHFIFVALSD"
     gene            391..1047
                     /locus_tag="ACSIGJ_000172"
     CDS             391..1047
                     /locus_tag="ACSIGJ_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HHQ1329166.1"
                     /translation="MEQKRRFAASLLLAAALPLCAHSFPFAEENPIAYGKVKIQSWKA
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQKLTEEEVLEKLGKEQMRASFEDMRRIMP
                     DLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVLRGVDGNTVLLADPSPGHVSM
                     SRAQFLEAWQTREGNLAGKILAVVPKKAEAISNKLFFTHHPKRQTEFAVGQIRQGRAE
                     "
     gene            1175..2470
                     /gene="hisS"
                     /locus_tag="ACSIGJ_000173"
     CDS             1175..2470
                     /gene="hisS"
                     /locus_tag="ACSIGJ_000173"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HHQ1329167.1"
                     /translation="MAQKIQSVKGMNDLLPVGQKDFKLTAVFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEAQLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENSRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELADGTVTLKDMNGAHGQQTVSATDLTDTLQQWKNA"
     gene            2471..3100
                     /locus_tag="ACSIGJ_000174"
     CDS             2471..3100
                     /locus_tag="ACSIGJ_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0044877 - protein-containing complex
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HHQ1329168.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELSKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQEKSQEALKNYGQALEKMPQDSVGRELLQMKLDSLK"
     gene            3250..4707
                     /gene="der"
                     /locus_tag="ACSIGJ_000175"
     CDS             3250..4707
                     /gene="der"
                     /locus_tag="ACSIGJ_000175"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HHQ1329169.1"
                     /translation="MKPTIALIGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYFVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGDRAVLAAEFYELALGEPHVISGAHGDGVYYLIEEIL
                     ENFPEPEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEKRVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDISRKLYFLDFAKFHFISALKERGI
                     DGLFESIQAAYNAAMIKMPTPKITRVLQTAVGRQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            4738..5637
                     /locus_tag="ACSIGJ_000176"
     CDS             4738..5637
                     /locus_tag="ACSIGJ_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HHQ1329170.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTRLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            5689..6106
                     /locus_tag="ACSIGJ_000177"
                     /pseudo
     CDS             5689..6106
                     /locus_tag="ACSIGJ_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6173..6739
                     /gene="dcd"
                     /locus_tag="ACSIGJ_000178"
     CDS             6173..6739
                     /gene="dcd"
                     /locus_tag="ACSIGJ_000178"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HHQ1329171.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGQRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEVCETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(6805..7509)
                     /locus_tag="ACSIGJ_000179"
     CDS             complement(6805..7509)
                     /locus_tag="ACSIGJ_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HHQ1329172.1"
                     /translation="MLRSVLAASLLAVSFPAAAEALNYNIVEFSESAGIEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKAEGRDFDALNRFIADVQTDASLEDTDFSVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNFGQIGSHIAGDGAVRAKMLRAMPMAASVNMKGTDSAA
                     PGVEEISISINGTVQF"
     gene            7641..7943
                     /locus_tag="ACSIGJ_000180"
     CDS             7641..7943
                     /locus_tag="ACSIGJ_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase group 1"
                     /protein_id="HHQ1329173.1"
                     /translation="MSEPLHVLVIPLWYPRSEQDVDGIFFQNQAQALQRKGIKTAILA
                     PMFRYLRKETASILTGPYGFARYRQSGLDIYAWRSMYFFPAFRLSTSTASAGCAPV"
     gene            7868..8263
                     /locus_tag="ACSIGJ_000181"
     CDS             7868..8263
                     /locus_tag="ACSIGJ_000181"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HHQ1329174.1"
                     /translation="MAQHVFLPRFPFIDIDRIRWVRAGLKAFKHYIRENGLPDLIHAH
                     CMNYAGILAQKISEKYGIPYVLTEHSSTITRGLIRHHQWQPMEKAAAPASARLAVSRH
                     FAHVLQHKYGCEWQYLPNIPGGIFKQTFE"
     gene            8291..8848
                     /locus_tag="ACSIGJ_000182"
     CDS             8291..8848
                     /locus_tag="ACSIGJ_000182"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HHQ1329175.1"
                     /translation="MFCTVSHLRRLKGHDVLLTAFARALAQCPQLRLNIGGSGQEEQR
                     LKQQAADLGIAHAVTFLGALQPEAILDLMRNSDAFILASRTETFGVVYIEALSQGLPV
                     IAIHCGGAESIVSDGNGYLVSVDDADVLAFLKIYEHHADFSAEQLRTDCLATFGEDAV
                     IGRLIAVFRQAARNTARKRPKNRLK"
     gene            8874..9827
                     /locus_tag="ACSIGJ_000183"
     CDS             8874..9827
                     /locus_tag="ACSIGJ_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HHQ1329176.1"
                     /translation="MTHTVHLHLEETDNPTLQRLCGSFDSNLDSLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDSIIRLAAVETQTEDAGHQEKNHEHAYYF
                     RTKRGSIGGRTPRQNGYIRALLDHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEITPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVAGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(9932..10255)
                     /locus_tag="ACSIGJ_000184"
     CDS             complement(9932..10255)
                     /locus_tag="ACSIGJ_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329177.1"
                     /translation="MKKCILGILTACAAMPAFADRISDLEARLAQLEHRVAVLESGGN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCGDEAIR
                     CRKFD"
     gene            complement(10428..11786)
                     /locus_tag="ACSIGJ_000185"
     CDS             complement(10428..11786)
                     /locus_tag="ACSIGJ_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HHQ1329178.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNISAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGNAAERYQEDPVRILRAIRLSGKLGFELSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDIHPLLNALRVSDGIAGKM
                     TALALKNTDERLRADKSVSVGFVLAALMWPELDRHWKSNLQQGLKPTPALSDAINTMR
                     ETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAETG
                     NADRALAEWWTAFQTASAEQRTEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGTD
                     WE"
     gene            complement(12144..13844)
                     /gene="recJ"
                     /locus_tag="ACSIGJ_000186"
     CDS             complement(12144..13844)
                     /gene="recJ"
                     /locus_tag="ACSIGJ_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HHQ1329179.1"
                     /translation="MSVKIQTRPVNTDVFNHLLAAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAAGRLADAVERKEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPADTVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLTALRAELRRRNYFSDGIKEP
                     NLGGLLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAARLNNLNIERREIEQSM
                     LRDALNAFPETLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTYITDGSLPACDITLEQAQNLACHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRDNLELQLYIDYWEAA
                     "
     gene            complement(13913..14626)
                     /locus_tag="ACSIGJ_000187"
     CDS             complement(13913..14626)
                     /locus_tag="ACSIGJ_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329180.1"
                     /translation="MLFRKTTAAVLAATLILNGCTMMLRGMNNPVSQTITRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFAVNPEDSAKLTGLLKAGLDKPFQIVEDTPS
                     YARHQALPVKFEAPGSQNFSTGGLCLRYDTGRPDDIAKLKQLEFKAVKLDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTEKHTDKSKLFGNILYTPPLLILDAA
                     AAVLVLPMALIAAANSSDK"
     gene            complement(14673..15179)
                     /gene="yjgA"
                     /locus_tag="ACSIGJ_000188"
     CDS             complement(14673..15179)
                     /gene="yjgA"
                     /locus_tag="ACSIGJ_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HHQ1329181.1"
                     /translation="MFEQEDEWISKTQMKKQMNGLQDLGMELTRLSNDTLKKIGLDED
                     LYEAVVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     QARVRLLADEGALTQFMSDFPNADAGRLRTLVRNTKKEQEQDKPPKNFRALFQELKTV
                     MENGGAEI"
     gene            15333..16664
                     /gene="pmbA"
                     /locus_tag="ACSIGJ_000189"
     CDS             15333..16664
                     /gene="pmbA"
                     /locus_tag="ACSIGJ_000189"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HHQ1329182.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGLAKQCEQAALDTDSRIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQSTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGTLSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLGSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(16963..17166)
                     /locus_tag="ACSIGJ_000190"
     CDS             complement(16963..17166)
                     /locus_tag="ACSIGJ_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HHQ1329183.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            17411..17713
                     /gene="clpS"
                     /locus_tag="ACSIGJ_000191"
     CDS             17411..17713
                     /gene="clpS"
                     /locus_tag="ACSIGJ_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HHQ1329184.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLSVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            17715..20006
                     /gene="clpA"
                     /locus_tag="ACSIGJ_000192"
     CDS             17715..20006
                     /gene="clpA"
                     /locus_tag="ACSIGJ_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HHQ1329185.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDAAVPNV
                     LKLCGADLKAVSEQLAASVAENTPLIPDHLLDTVETRPTLGFQRVIQRAMVHTQSAGK
                     GLAEPLDVLVALMSETDSHAVYFLKLQSVTRFEVLRCIAHGSPDEDEDDGNYFSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALADGLAHQIVNDDIPDALKEAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTGGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVAETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLGRDLNNMVYGQEDAIDALVSAVKMSRSGLGLPDKPIGSFLFSGPTGVGKTE
                     VAKQLAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGF
                     TAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHRLLDKK
                     VEAEFTPALRKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLADGGFVRIDW
                     DAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(20046..23144)
                     /locus_tag="ACSIGJ_000193"
     CDS             complement(20046..23144)
                     /locus_tag="ACSIGJ_000193"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HHQ1329186.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPAENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVVVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLSVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSGPESGGYVWHTTGSGKTLTGFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSESTAGLKRNLEKDGNKIIVTTIQKLNNLMKS
                     EDNLPVYHRQVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKELSAAENRQALL
                     HPERIREITQYILSRFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLH
                     PLKTATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNREVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNIVLEKSYEEYMNGYTDSQTG
                     EARRGYLDVAKELHERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QNVDAADEDAMKAFQEKYYLSDEDVQEMRKVPMPSERAVQDYRSAYNDIRDWLRRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEGLNETA
                     AKRYLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGA
                     DI"
     gene            complement(23375..24597)
                     /locus_tag="ACSIGJ_000194"
                     /pseudo
     CDS             complement(23375..24597)
                     /locus_tag="ACSIGJ_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359898.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(24764..25615)
                     /gene="dinD"
                     /locus_tag="ACSIGJ_000195"
     CDS             complement(24764..25615)
                     /gene="dinD"
                     /locus_tag="ACSIGJ_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HHQ1329187.1"
                     /translation="MTTENNAFENAKHIDETGNEYWSARTLQQILEYSEWRNFQRAID
                     KAITACETSGNDKNHHFVETNKMIALGKGGQREVADYRLSRYACYLIVQNGDPSKSVI
                     AAGQTYFAVQARRQELQDEAAFRSLGEDKQRLLLCRQLREHNTDLAAAAKDAGVEKPV
                     EYAVFQNHGYRGLYGGLDKQGIHSRKGLKKSQRILDHMNASEPAANLFRATQTEEKLR
                     RKNIQGKTQANRVHFEVGQKVRQTIEELGGIMPENQPVPEKSIKQLENEEQKRLAATE
                     QHQNGKK"
     gene            complement(25731..27283)
                     /locus_tag="ACSIGJ_000196"
                     /pseudo
     CDS             complement(25731..27283)
                     /locus_tag="ACSIGJ_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
     gene            complement(27358..28362)
                     /gene="rfaD"
                     /locus_tag="ACSIGJ_000197"
     CDS             complement(27358..28362)
                     /gene="rfaD"
                     /locus_tag="ACSIGJ_000197"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HHQ1329188.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLTKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKINLYFFDHPELSGIYNLGTGRSQQFNELAAAAVNACRAAEGKSELSLKEL
                     VEEELIRYIPFPDALKGKYQGFTQADITKLREAGYKEEFFDVKAGVNRYVKWMLENLA
                     "
     gene            complement(28426..29388)
                     /gene="hldA"
                     /locus_tag="ACSIGJ_000198"
     CDS             complement(28426..29388)
                     /gene="hldA"
                     /locus_tag="ACSIGJ_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HHQ1329189.1"
                     /translation="MPAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGDDEAAAALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNREVLEQIKRKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHVGKTVLIDPKGDDYEKYVGATLITPNCAELKEVVGSWKNEGDL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELVEALDGQ"
     gene            complement(29444..30184)
                     /gene="pyrF"
                     /locus_tag="ACSIGJ_000199"
     CDS             complement(29444..30184)
                     /gene="pyrF"
                     /locus_tag="ACSIGJ_000199"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HHQ1329190.1"
                     /translation="MNPLITDFQTPQQRTPVIVALDFANEKDTLGFVRNLDPALCQIK
                     IGKELFTATGRSLAESLIHQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGRDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTRAADPVAVLREVNRVANLEAN"
     gene            complement(30553..31200)
                     /gene="adk"
                     /locus_tag="ACSIGJ_000200"
     CDS             complement(30553..31200)
                     /gene="adk"
                     /locus_tag="ACSIGJ_000200"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HHQ1329191.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVG
                     LDAVVEIDVSDSVIVDRMSGRRVHLASGRTYHVTYNPPKTEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQAVEAVKAEVLGALGK"
     gene            31415..32254
                     /gene="htpX"
                     /locus_tag="ACSIGJ_000201"
     CDS             31415..32254
                     /gene="htpX"
                     /locus_tag="ACSIGJ_000201"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HHQ1329192.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(32490..33788)
                     /locus_tag="ACSIGJ_000202"
     CDS             complement(32490..33788)
                     /locus_tag="ACSIGJ_000202"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HHQ1329193.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHEGLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQYLHNAGPVKAEDVMAVIEKVAPRIAPMIADVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPGGGKTDILPCGSDAVETCKPIYETMPGWRESTVGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(33893..35044)
                     /locus_tag="ACSIGJ_000203"
     CDS             complement(33893..35044)
                     /locus_tag="ACSIGJ_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HHQ1329194.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLPNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGSVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALDELQAVCDAFPDNEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAVERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQIVKR"
     gene            35379..36758
                     /locus_tag="ACSIGJ_000204"
     CDS             35379..36758
                     /locus_tag="ACSIGJ_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     NorM"
                     /protein_id="HHQ1329195.1"
                     /translation="MLLDLDRFSFSVFLKEIRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTGEAGETGRQGIW
                     FGLILGIFGMILMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGVATMAVFWFSALALW
                     IYIAKEKFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVSGWVL
                     AVITVLSLVLFRSPLASMYNDDPAVLSIASTVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAVALVWCLEKYSM
                     ELVKSHKAV"
     gene            36802..37842
                     /gene="murB"
                     /locus_tag="ACSIGJ_000205"
     CDS             36802..37842
                     /gene="murB"
                     /locus_tag="ACSIGJ_000205"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HHQ1329196.1"
                     /translation="MQPIRYRTDLTPYNTFGLHAQARAFIALKHADELRDIVRLPEFD
                     RDTVLWLGGGSNILLMQDYDGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHT
                     VALGLSGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAKVAELSAGREATAKDVS
                     DAVSAIRNSKLPDPKVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLANKNNASSDDIWKLAQHVCNTVFTRFQVELH
                     AEPNWLPASFSL"
     gene            37854..38504
                     /locus_tag="ACSIGJ_000206"
     CDS             37854..38504
                     /locus_tag="ACSIGJ_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HHQ1329197.1"
                     /translation="MPVTRIAKTNTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVKLLTQLNGLNVINADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGVRRTLSLLRPYLLPEHRAEYDRKISNGNP"
     gene            38559..39335
                     /locus_tag="ACSIGJ_000207"
     CDS             38559..39335
                     /locus_tag="ACSIGJ_000207"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HHQ1329198.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYRHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLNRTDTFQTALWRHHADKPVWFFLLPPSFLAHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDKARECNETAAPDPQTESARQILAAEQHLLDNGVPNIDILRLGG
                     LYCAERHPVSRLVQKQNIQGGNRPVNIVHRDIAVENLFQTASNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFSPDDSVGKIIRTVCDNGLSL"
     gene            39385..39951
                     /locus_tag="ACSIGJ_000208"
     CDS             39385..39951
                     /locus_tag="ACSIGJ_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110142 - ubiquinone biosynthesis complex
                     [Evidence IEA];
                     GO_function: GO:0008289 - lipid binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiJ"
                     /protein_id="HHQ1329199.1"
                     /translation="MSALLPIINRLILQSPDSRSELTSFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAIRKILQGGEPGAGDIRLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQAGIGSRATDIGHGIKQIGRNIAEQIGGFSREPESANTGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            39971..40861
                     /locus_tag="ACSIGJ_000209"
     CDS             39971..40861
                     /locus_tag="ACSIGJ_000209"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HHQ1329200.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGV
                     RECCIYTQDTDGCHIVNKTELGQYCDLVAVLGGDGTFLSAAREITPRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERALALNDAVLSRGG
                     AGQMIEFEVFVNQEFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQSFIDVQNLDRIIIRRYHNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            40886..41644
                     /locus_tag="ACSIGJ_000210"
     CDS             40886..41644
                     /locus_tag="ACSIGJ_000210"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HHQ1329201.1"
                     /translation="MNSKISSDHTEDTIRLSKRMAQLGLCSRREADGHIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NCWEGDTSRISFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGDKLRPAKVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(41699..42363)
                     /locus_tag="ACSIGJ_000211"
                     /pseudo
     CDS             complement(41699..42363)
                     /locus_tag="ACSIGJ_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            complement(42560..43333)
                     /gene="folE2"
                     /locus_tag="ACSIGJ_000212"
     CDS             complement(42560..43333)
                     /gene="folE2"
                     /locus_tag="ACSIGJ_000212"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HHQ1329202.1"
                     /translation="MNAIADVQSSRDLRNLPINQVGIKDLRFPITLKTAEGTQSTVAR
                     LTMTVYLPAEQKGTHMSRFVALMEQHTEVLDFAQLHRLTAEMVALLDSRAGKISVSFP
                     FFRKKTAPVSGIRSLLDYDVSLTGEMKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHN
                     QRSHVTVSLTSDAEVGIEEVIDYVETQASCQLYGLLKRPDEKYVTEKAYENPKFVEDM
                     VRDVATSLIADKRIKSFVVESENFESIHNHSAYAYIAYP"
     gene            43394..43540
                     /locus_tag="ACSIGJ_000213"
     CDS             43394..43540
                     /locus_tag="ACSIGJ_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329203.1"
                     /translation="MPSEKTLSAVLPANAGLYHLLLAAALIVSIFGIVIKSRLIHILQ
                     GRNR"
     gene            43537..44694
                     /locus_tag="ACSIGJ_000214"
     CDS             43537..44694
                     /locus_tag="ACSIGJ_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HHQ1329204.1"
                     /translation="MKFPTRAIHSGYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHNVKLVWLEMPSNPLLRLVDIKALAA
                     KAKAADALVGIDNTFATPYLQQPLDMGCDFAFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAIAGPTDCWLVLRGIKTLALRMEAHCKNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYALAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMTSSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIRVGLLRFSIGIEDVDDIWNDISAALDTTL"
     gene            complement(44758..45759)
                     /gene="hemB"
                     /locus_tag="ACSIGJ_000215"
     CDS             complement(44758..45759)
                     /gene="hemB"
                     /locus_tag="ACSIGJ_000215"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HHQ1329205.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHMLTADDLIYPVFVLE
                     GAAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTGRAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGTQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(45943..46227)
                     /gene="yhbY"
                     /locus_tag="ACSIGJ_000216"
     CDS             complement(45943..46227)
                     /gene="yhbY"
                     /locus_tag="ACSIGJ_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HHQ1329206.1"
                     /translation="MADTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICNTLCEAVDAQPVRHIGKLLVLWRKNIEA"
     gene            46337..46957
                     /locus_tag="ACSIGJ_000217"
     CDS             46337..46957
                     /locus_tag="ACSIGJ_000217"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HHQ1329207.1"
                     /translation="MAVRSKSSKAWLHEHINDHYVHMAQKDGYRARAAYKLLEINEKD
                     KIIKPGTVLADLGSAPGSWSQVAAKLTGTSGTVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            47021..48988
                     /gene="ftsH"
                     /locus_tag="ACSIGJ_000218"
     CDS             47021..48988
                     /gene="ftsH"
                     /locus_tag="ACSIGJ_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HHQ1329208.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIRQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADTAEDN
                     APHAPTREKTEAPAPADTASTESGQQPENKA"
     gene            49210..49641
                     /locus_tag="ACSIGJ_000219"
     CDS             49210..49641
                     /locus_tag="ACSIGJ_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HHQ1329209.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMRVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            49634..49912
                     /locus_tag="ACSIGJ_000220"
     CDS             49634..49912
                     /locus_tag="ACSIGJ_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HHQ1329210.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRTAALQSGLDGIFENLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            49965..50543
                     /gene="pth"
                     /locus_tag="ACSIGJ_000221"
     CDS             49965..50543
                     /gene="pth"
                     /locus_tag="ACSIGJ_000221"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HHQ1329211.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSAEHR
                     RQIDDAVAKSLQAVPDIISGKWEEATRFLHSK"
     gene            50604..50810
                     /locus_tag="ACSIGJ_000222"
     CDS             50604..50810
                     /locus_tag="ACSIGJ_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HHQ1329212.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            50807..51169
                     /locus_tag="ACSIGJ_000223"
     CDS             50807..51169
                     /locus_tag="ACSIGJ_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HHQ1329213.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQILLLAKAFKTGKLPIPYRSLIAFAFCFAVGSECAQAWFTATRTGSLGDVLAD
                     LTGAALALFAARSACRPD"
     gene            51398..52813
                     /locus_tag="ACSIGJ_000224"
     CDS             51398..52813
                     /locus_tag="ACSIGJ_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HHQ1329214.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITITALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFGSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIAASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            52933..53442
                     /locus_tag="ACSIGJ_000225"
     CDS             52933..53442
                     /locus_tag="ACSIGJ_000225"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HHQ1329215.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSAGAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIESVSTKRHGYHDDVPTEP
                     VIIIKAEAV"
     gene            53610..54992
                     /locus_tag="ACSIGJ_000226"
     CDS             53610..54992
                     /locus_tag="ACSIGJ_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HHQ1329216.1"
                     /translation="MASITRDIFKAYDIRGIVGKTLTDDAAYFIGRAIAAKAAEKGIA
                     RIAIGRDGRLSGPELMEHIQRGLTDSGIGVLNVGMVTTPMLYFAAVNECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIVEKDGFVAADKQGSVTEKDISGAYHDHI
                     VGHVKLKRPINIAIDAGNGVGGAFAGKLYKGLGNEVTELFCEVDGNFPNHHPDPSKPE
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLNRNPGAK
                     VIFDVKSTRLLAPWIKEHGGEAIMEKTGHSFIKSAMKKTGALVAGEMSGHVFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLDNLPQSISTPELNISLPEGSNGHQVIEELAAK
                     AQFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQAAIERIQNRFKAVI
                     ESNPHLIWPL"
     gene            complement(55048..55803)
                     /locus_tag="ACSIGJ_000227"
                     /pseudo
     CDS             complement(55048..55803)
                     /locus_tag="ACSIGJ_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(55813..56529)
                     /locus_tag="ACSIGJ_000228"
     CDS             complement(55813..56529)
                     /locus_tag="ACSIGJ_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HHQ1329217.1"
                     /translation="MFNNFLASLPFMTETRADMLISAFWPMVKAGFTVSLPLAIASFV
                     IGMIIAVAVALVRIMPSGGIFQKCLLKLVEFYISVVRGTPLLVQLVIVFYGLPSVGIY
                     INPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(56519..57346)
                     /locus_tag="ACSIGJ_000229"
     CDS             complement(56519..57346)
                     /locus_tag="ACSIGJ_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HHQ1329218.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAISGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSEPYSWSGAVLVARNDSNIKSIADIKGVKTA
                     QSLTSNYGEKAKAAGAQLVPVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            57631..58539
                     /locus_tag="ACSIGJ_000230"
     CDS             57631..58539
                     /locus_tag="ACSIGJ_000230"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HHQ1329219.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFPEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDETIREVAI
                     IPEGLPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRSKETDDEFSRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            58616..62218
                     /gene="recB"
                     /locus_tag="ACSIGJ_000231"
     CDS             58616..62218
                     /gene="recB"
                     /locus_tag="ACSIGJ_000231"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HHQ1329220.1"
                     /translation="MSAPIRAFDPLTAPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKSVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAELGDDTLSDGIAAYCAE
                     HHEGDTFLPALLEQALQKESRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLVPAQDFWRERVSGDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQRDAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHERLLKLSSDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEETTMIRLQLDLIEYLNSSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     PARAVAENWEIALIDEFQDTDPLQYEIFQKIFIARNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQLRWLHENDNEKANKDVLRRRAADYCADEIARALNEASGGRLNFKGRPLQ
                     SGDIAVLVRTHNEAVMISAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDIWQKYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNGGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQGANRAELLAK
                     AQLSESEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRRTYACEKDGITMLKRNWRRVADNAPAGTDFAFTEDAPPPAAYRGNADQAAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAEQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYGLSDIPPGCRRPEMGFTLHTEDFGLKRLRDWLARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICIIDYKSNHLSAYTRQAMDEAVAHQHYYL
                     QALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK"
     gene            62257..62616
                     /locus_tag="ACSIGJ_000232"
     CDS             62257..62616
                     /locus_tag="ACSIGJ_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HHQ1329221.1"
                     /translation="MNIKQLITAALIASAAFATQAAPQKPVSAAQTAQHSAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIYEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            62741..63220
                     /locus_tag="ACSIGJ_000233"
     CDS             62741..63220
                     /locus_tag="ACSIGJ_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HHQ1329222.1"
                     /translation="MKHILPLIAASALCISTASAHPADKPPTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRTAYTDTRALIVGSRISFDEVANTLANAEKLIQKTVGE"
     gene            complement(63495..64874)
                     /gene="radA"
                     /locus_tag="ACSIGJ_000234"
     CDS             complement(63495..64874)
                     /gene="radA"
                     /locus_tag="ACSIGJ_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HHQ1329223.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESFAAPEPK
                     NARFQSWAADTSTVQSLSAVTAAEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRSQRLELHAEGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLSVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIHGVSSLQEA
                     IDICRDSRE"
BASE COUNT        15279 a        18554 c        16482 g        14766 t
ORIGIN      
        1 aatgccgtac cggtttaaat ttaatccact atatatttat ccgatatatc aacatctctc
       61 tatcttattc cgaattttat tgttgaaact tgcattaaaa accatacaag atgatgaaga
      121 acaaatatat tttaataaaa gcagcaatcg gcataaccgc catatccata ttttatcttc
      181 tgactcaagg aagcatcggc aaaactgaag aaccatcata tttcttgatg ttcatgtttc
      241 tcaactcgct ttggtttgaa gaaaataaaa cagttatggc tgccgttacg gcaatgattg
      301 ccgcccactt tatatttgtc gcactatccg attaataagc aaacaaaaag gctgcttgga
      361 aatttcaggc agcctttcag gaggatttta atggaacaaa aaaggcggtt tgccgcctcc
      421 ctgctcctcg ccgccgccct gccgctttgt gcacattctt ttccctttgc ggaagaaaac
      481 cccatagcct acggcaaagt caaaatacag agttggaaag cgcggcggga tttcaatatt
      541 gtaaagcagg atttggattt ttcctgcggg gcggcttcgg tggcgacact tttgaacaat
      601 ttttacgggc aaaagctgac ggaagaagaa gtgttggaaa aactgggtaa ggaacagatg
      661 cgcgcgtcgt ttgaggatat gcggcgcatt atgcccgatt tgggttttga ggcgaagggc
      721 tatgccctgt ctttcgagca gctcgcgcag ttgaaaatcc ccgtcatcgt gtatctgaaa
      781 taccgcaaag acgaccattt ttcggtattg cgcggagtgg atggcaatac ggttttgctt
      841 gccgacccgt cgccgggtca tgtttcgatg agcagggcgc agtttttgga ggcttggcaa
      901 acccgtgagg gaaatttggc aggcaagatt ttggcggtcg tgccgaaaaa agcggaggcg
      961 atttcaaata aattgttttt cacacatcat cccaagcggc agacggagtt tgcagtcgga
     1021 caaatcaggc aagggcgcgc agagtaagtc aaatgccgtc tgaaaactgt ttcagacggc
     1081 atttttaccc gtccgcaaac catctccatc ctcacaagcc cttaaaaatc cgctataatc
     1141 gcccgcaatt tgatttcagc accccctttt tgaaatggca caaaaaatcc aatccgtcaa
     1201 aggcatgaac gaccttctgc ctgtcgggca aaaagatttc aaactcacgg ctgtgttttg
     1261 gcaggcgttt gaggatacgg taaaccgttg gacgcgagct tacggttatc agcaaatccg
     1321 tacgccgatt gtcgagcaaa ccggtttgtt tgtccgctcc atcggcgagg aaaccgatgt
     1381 agtcggcaag gaaatgtaca ccttctccga ttcaaacgat tctttgagtt tgagcctgcg
     1441 tcccgaaggt acggcctctt gtctgcgtgc ggtggtcgaa cacaaccttc tgtacaacag
     1501 cccgcaaaag ctgtggtata tggggccgat gttccgccgc gagcgtccgc aaaaaggacg
     1561 ctaccgccaa tttcatcagg tcggtatcga ggctttgggt tttgaagggc cggatattga
     1621 tgcggaaatc atcgcaatgt ctgccgactt gtgggaaaaa ttgggtattc gcgaatacct
     1681 gactttggaa atcaacagct tgggcaaccg tgaggaacgc gcggcacacc gtgcggcatt
     1741 ggttgaatat ctgacccgct atgaagcgca actggacgaa gacagcaaac gccgtctgaa
     1801 aaccaatcct ttgcgcgttt tggatacgaa aaaccctgat ttgcaggaaa tctgcaatgc
     1861 cgcgccgcgt ttggtagatt acttgggcga ggcttcgcaa aaccactatg cacgcttcaa
     1921 ggcgatgttg gacggtttgg gtattcaata tattgaaaat tcgcgcttgg ttcgcggttt
     1981 ggattattac aatcagacgg tttttgagtg gacgaccgac aaactcggcg cacaggcgac
     2041 tgtgtgcggc ggcggacgtt acgacggttt gattgaagaa ctcggcggca agcctgcgcc
     2101 gtccatcggc tttgcgatgg gcatcgaacg gctgctgctt ttggtgagcg aatacggttc
     2161 gctggaagtg aacgccgcgc ctgatgtcta tgcaatgcac caaggcgaag gggcggactt
     2221 gcaggtgatg aaatacgcac aagccttgcg cgcgcaaggt ttcaatgtaa tgcagcattc
     2281 cggctatcaa agcctgaagg cgcaaatgaa aaaagccgac aacagcggcg cgcgctttgc
     2341 cctgattgtc gcgcaagacg aactggcgga cggtaccgtt acgctcaaag acatgaacgg
     2401 cgcacacggt cagcaaaccg tttccgccac cgatttgaca gatactttac aacaatggaa
     2461 gaacgcataa atggcagccc atctcgaaga acaacaagag ttagacaact ttaaatattt
     2521 ttggaaaacc acgggcaaat ggctgtttgc cctgctgatt ttggcggcac tcggctactt
     2581 gggatacacg gtttaccaaa accgtgcggc ttcccaaaat caggaagcgg cggcggtgct
     2641 ggcaaacatc gtggaaaagg cgcaaaacaa agccccgcaa agcgaaatca atgccgaact
     2701 gtccaagctc caacaaagct acccccattc catttccgcc gcccaagcca cgctgatggc
     2761 ggcggcaacc gaatttgacg cgcagcgtta cgatgttgcc gaaggtcatt tgaaatgggt
     2821 gttgtccaac caaaaagaca gcctgattca ggcgttggcg gcgcagcgtc tgggcgttgt
     2881 gttgttgcaa caaaaaaaat acgatgccgc gcttgccgca ctcgacacgc cggttgaggc
     2941 ggacttcgcc cccctgctga tggaaactaa aggcgatgtt tatgccgcac aggaaaaaag
     3001 ccaggaagcc ttaaaaaact acggacaggc tttggaaaaa atgcctcaag attctgtcgg
     3061 tcgcgaattg cttcaaatga aactcgattc gctgaaataa ataccgtctg aaatgccgct
     3121 ctctcttcag acggcatttc agacaacccc atcctacggc aattcccgta aaaccatccg
     3181 ccgtgtccca acggacacct gctcgtgttc agttctaccg aacggcactt gacacagaaa
     3241 gacccaatca tgaaaccaac catcgcgctt atcggccgcc ccaacgtcgg caaatctacc
     3301 ttgttcaacc gtttgacgcg caccaaagac gcgctcgtgc atgacctgcc gggtctgacc
     3361 cgcgaccgcc attacggaca cggcaaagtc ggcagcaaac cttattttgt catcgatacc
     3421 ggcggtttcg agccggttgt ggacagcggc attttgcacg aaatggcaaa acaaaccttg
     3481 caggctgtcg atgaagccga tgcggttgtg tttttggtgg acggccgtac cggtttgaca
     3541 ccgcaagaca agattattgc cgaccgtttg cgccaaagtc cgcgtcccgt ttatttggcc
     3601 gtgaataaag gcgagggcgg cgacagggcc gtacttgccg ccgagtttta cgagctggca
     3661 ttgggcgagc cgcacgttat ttccggcgcg cacggcgacg gcgtgtatta cctgattgaa
     3721 gaaattttag aaaacttccc cgagcctgaa gccgaagaag ccgatgcaaa acatcccgtt
     3781 tttgccgtta tcggtcgtcc gaacgtcggc aaatctacgc tggttaacgc cattctcggc
     3841 gaaaaacgcg tcatcgcctt cgatatggca ggcacaacgc gcgacagcat ccacatcgat
     3901 ttcgagcgcg aaggcaaacc gtttaccatc atcgataccg caggcgtgcg ccgtcgcggc
     3961 aaagtggatg aagcggtgga aaagttctcc gttatcaaag ctatgcaggc ggttgaagcg
     4021 gcaaacgttg ctgttttggt attggacgcg caacaggaca ttgccgacca agatgcgacg
     4081 attgccggtt ttgctttgga agcgggacgc gcgctggtag ttgccgtcaa caaatgggac
     4141 ggcatcagcg aagagcggcg cgaacaagtg aaacgcgaca tttcccgcaa actgtatttc
     4201 ctcgattttg ccaagttcca ctttatttcc gcgttgaaag agcgcggtat agacggtttg
     4261 tttgaaagca ttcaagctgc ctacaacgcg gcgatgatta agatgccgac gccgaaaatc
     4321 acgcgcgtat tgcaaaccgc cgtcggacgc caacagccgc cgcgtgccgg cttggtgcgt
     4381 ccgaaaatgc gttatgccca ccaaggcggc atgaatccgc cggtgattgt ggtacacggc
     4441 aattcgctgc acgcgatttc cgacagctat acgcgctatc tgacccagac cttccgcaaa
     4501 gccttcaacc tgcaaggcac gccgttgcgg attcaataca atgtttcgga aaacccgtat
     4561 gaaaatgcgg aagacaaacc gaagaaaaaa ccgctgcgcc gcgtcagcct gagcaaccgt
     4621 attgagaaac gcgaaggccg aaaggaagag aaaaaccgct tcaagaagaa aaccaaagtc
     4681 agtgtgaaaa aacaattcag caaataattc ccgacatttc aaacaatagg aaacattatg
     4741 tggttcaagc agattagttt ttatccgctc aacaaagaaa agctgcctga ggcggacgta
     4801 cttgccgata aacttgctga agctgaattt acccattgcc aaggtttgga ctggttcagc
     4861 gaaggcttta ccgcaccggt ttctttttcg cccgaactcg ttttccctgc cgactttacc
     4921 ttgcgcgtcg ccctgaaaaa agaggaaaaa gtcctgcctg ccggcgtcat ccgcgatatt
     4981 ttggaagaga aggtagcgga aatccaaaac aatgaagccc gcaatgtcgg tcgcaaagaa
     5041 aaacaagagc ttaaagagca aattacagac gacctgctgc cccgagcgtt tacccgcagc
     5101 agccgtacag aagcggtgtt taacacccgc cacggctacc tgctcgtcaa taacgcggct
     5161 tccgccaaag cggaaaacat cctgaccaag ctgcgcgaag ctttgggagg tttggaagcc
     5221 tcgctgccga ataccaaaca atcgccctct tccctgatga ccggctggct gttgcaaggg
     5281 cattgcgaag gcggttttga attagacagc gattgcgaac tcaaaggtac gggcgatatt
     5341 gttcccgtcg tcaaagtatc caaacaagac ttaaccgccg acgaagtggt tcaacacgtc
     5401 aaaaacggta aaaccgttac ccggctcggt ttggtttggc gcgaacaaat cgcctttatc
     5461 ctcactcaag acttcacact caagcgcatc caatacctcg acgtattgca ggaagaagcc
     5521 gaaagcaacg gcgacgatgc cgccggcctt gccttcgcct cgcaaatcct gatggcggaa
     5581 tccgtcagca ccatgttgga agagctggtt tcctatttgg gcggctggca agattgattt
     5641 ttcagacggc ttttataccg tcagccaaaa tccaatggaa taacccttat gcttaaacat
     5701 ctcgcattcc tactgcccgc catgatgttc gccctccccg cccagaccgc cgtcctaagc
     5761 ccctatcagg aaaccggctg cacctacgaa ggcgggatcg gaaaagacgg gcttccttca
     5821 ggcaaaggca tatggcgttg ccgggatggg cgcggttata ccggttcatt caaaaacggc
     5881 aaattcgacg ggcaaggcgt ttataccgtt gccgccggcc gcgaagtatt tctcgagccg
     5941 ttcaattccg acagtaccaa attccgcaat atggcattgt cgggcacgtt caaacaaggc
     6001 ttggcacacg gcaggttcgc cgcctcgcaa aacggcgaaa ccctctttta ttatgaaatg
     6061 cgaacacggc atgattaaag aagtgaaact gcccaaaaac aaataacccc ccgtttttca
     6121 aacagcatcc cccaatgctt tatccgaaca ccgcaaaaaa caaggaaacc ccatgagcat
     6181 caagtccgac aaatggatac gccgaatgag cgaagaattc ggcatgattg atcccttcga
     6241 gccgaaccaa atcaaagaag ccgacggaca gcgcatcatc tcctacggta cgtccagcta
     6301 cggctacgat atccgctgcg ccaatgaatt taaaattttt accaacatca acagcaccat
     6361 cgtcgatccc aaaaacttcg acccgaaaaa tttcgttacc gtcgaagacg actgctgcat
     6421 catcccgccc aattccttcg cactggcgcg cacggtcgaa tatttccgca ttccgcgcaa
     6481 cgtcctgacc gtctgtttgg gcaaatccac ctacgcccgc tgcggcatca tcgtcaacgt
     6541 taccccgttc gagccggaat gggaaggcta cgttaccctc gagttttcca acaccacccc
     6601 cctgcccgcc aaaatctacg caggcgaagg cgtggcgcaa gtcctcttct tcgagagcga
     6661 cgaagtgtgt gaaacttcat acaaagaccg caacggcaaa tacatgggac aaaccggcgt
     6721 aaccctgccc aaagcctgaa atacaaaatg ccgtctgaac ccttatcgtc gggtttcaga
     6781 cggcatctac ctatttatcc gtggttagaa ctgaaccgtc ccattgatgc tgatgctgat
     6841 ttcctccaca cccggtgcgg ctgaatccgt acccttcata ttgacgcttg ccgccatcgg
     6901 catcgcgcgc agcatttttg cccgaacagc cccatcgccc gcaatatggc tgccgatttg
     6961 cccaaaattc aatttgacga ttttataacc ggacgcaccc agaacgcccg ccagtttttc
     7021 ggcacgcgcc ttgaaacgca aaacggcatc cttgctgacc tgatcgatga cctcgtttcg
     7081 gcgttcgcgc gacacgctga aatcggtatc ttcaagcgaa gcatccgtct gaacatcagc
     7141 aataaaacgg tttaaagcat caaaatccct gccctccgcc ttaaattccg cacgctcctc
     7201 ccagcctgtt tgaatgcgtc tgccgttggt atattgatag cgcggcatcg cactgcgcga
     7261 taccaattcg gttttaaagc taccattttt cgattttctg gtaaaattgt tgaatttttt
     7321 aacaaactcg gcattgacgg catttttgtc ccgtccttcc gccgccacct ggaaacgcgc
     7381 ggacattgta tcctgagcca cctcgatacc cgccgattcg gaaaattcga caatattgta
     7441 attcaatgcc tcagccgccg ccggaaaaga taccgccagc agggaagccg ccaaaacaga
     7501 acgcagcata tccgtatcct tacttgaaaa accgccaatc taacatatag gtacgctaaa
     7561 ttgcgttagc ataagacaac aatttaaata ttcgatcggt ctgccttata ataaccgtct
     7621 ttccgatact gaatcatatt atgtccgaac cgttgcacgt cctcgtcatc cccttatggt
     7681 atccacgatc cgaacaggat gtggacggaa tatttttcca aaatcaagca caggccctcc
     7741 aacgcaaagg catcaaaacc gccatcctcg cccccatgtt ccgttatctg cggaaagaaa
     7801 cagcaagcat cctgaccggt ccttacggtt ttgcccgata ccggcaaagc ggtttggaca
     7861 tctacgcatg gcgcagcatg tatttcttcc ccgctttccg tttatcgaca tcgaccgcat
     7921 ccgctgggtg cgcgccggtt tgaaagcctt caaacactac atccgcgaaa acggactgcc
     7981 cgacctgatt cacgcccact gcatgaacta tgcaggcata cttgcccaaa aaatttccga
     8041 aaaatacggc attccctacg ttttaaccga acacagcagc accatcacgc gcggtctgat
     8101 ccgtcaccac caatggcagc cgatggaaaa agcagcggcg cccgcctccg cacgtctcgc
     8161 cgtcagccgc catttcgcac acgtcctgca acacaaatac ggctgcgaat ggcagtacct
     8221 ccccaacata ccgggtggaa tattcaagca aacatttgaa taaaaagaaa aaaataacaa
     8281 accgcatttc gtgttctgca ccgtctcgca ccttcgccgc ctcaaaggac acgatgtcct
     8341 gctcactgct tttgcccggg cgttggcaca atgcccgcaa ctgcgcctga acatcggcgg
     8401 cagcggacag gaagaacagc ggctgaaaca gcaggcggca gacttgggca tcgcccatgc
     8461 cgttacattt ttaggcgcac tgcaacccga agcaatcttg gatttgatga ggaacagcga
     8521 cgccttcatc ctcgccagcc gcaccgaaac cttcggcgtg gtctatatcg aagccctctc
     8581 gcaagggttg cccgtcatcg ccatacattg cggcggcgcg gaatccatcg tttcagacgg
     8641 caacggatac ctcgtttccg ttgacgatgc cgatgtccta gcctttctca aaatatatga
     8701 acaccatgcc gatttttccg ccgaacagct cagaaccgac tgcctcgcaa cattcggcga
     8761 agatgccgtt atcggcagac tgattgccgt attccgtcag gcggcgcgga acacggcgcg
     8821 aaaacgcccg aaaaatagat taaaatgacg caaaacaacc ttcggattca aatatgaccc
     8881 ataccgtcca cctgcacttg gaagaaaccg acaacccgac gctgcaacgc ctgtgcggtt
     8941 ctttcgacag caacctcgat tcgctcgcca aagcactcga catccatatc agccgccgtt
     9001 tcgagcattt taccttcaac ggtgcatttg cacacgccgg caaacgcgca ctgctcaaac
     9061 tcttggaaac ggcgcagacg cgcgacctaa acgacagcat catccgcctt gccgccgtcg
     9121 aaacccaaac cgaagatgcc gggcatcagg aaaaaaatca cgaacacgcc tattatttcc
     9181 gaaccaagcg cggcagcata ggcggcagaa cgccacggca gaacggctat atccgcgccc
     9241 tgctcgacca cgacatcgta ttcggcctcg ggccggcagg tacgggcaaa acctatcttg
     9301 ccgtcgccgc cgccgtcgat gcgatggaaa aacaccaagt cgaacgcatc attttagtgc
     9361 ggcctgccgt cgaagccggt gagaaactgg gcttcctgcc cggagacctg acccaaaaag
     9421 tcgatcccta cctccgtccg ctttatgatg ccctctatga cctgatgggc tttgaccgtg
     9481 taaccaagct gattgaaaaa ggcctgattg aaatcacccc gctcgcctat atgcgcggca
     9541 ggacgctcaa cggcgcatac atcatcctcg acgaagcgca aaacaccacg cccgaacaaa
     9601 tgaaaatgtt cctgacccgc atcggcttcg gcgcgaaagc cgtcattacc ggcgacacca
     9661 gccaaatcga cctgcccaaa aacatcaaat cgggattgaa agacgcacgt gagaaactgc
     9721 acggcgtggc aggactgtat ttccatacct tcaccggcga agacgttgtc cggcatcctc
     9781 tggtgcaaaa aatcgtcgaa gcctacgaat cggcagaaca cgactgacac tccaatgccg
     9841 tctgaaaccc gatgcatgga aaatcgggca gagaaaaaca gagacggaag ccaagctccg
     9901 tatccgtttc tttatccgtc caaccgatac atcaatcgaa ttttctgcat cggattgcct
     9961 catctccgca aaacattgcc gaagtttcgc ggttgcacgc ctgacgcact ttctgccgcg
    10021 ccacgccttc attccgatcg cttgcctcaa acgtcttctg aaaaggcgta acgctgcata
    10081 catacatggt ggaatttgaa ccgaaaaggt cgattttgac ggtattgccg ccgctttcca
    10141 atacggcgac acggtgttcc aactgcgcca gacgtgcttc caaatcgctg attctgtcgg
    10201 caaatgcagg catggcggca cacgcggtca aaatgcccaa aatacatttt ttcatcaatt
    10261 tttccttgtt gcttgaaccc tgcccacccg gacatccttt ttccgggcgg cagggtcaga
    10321 ctttatttgc agggatgaag ccccttacaa atcagaataa tacatgaatc ggcactttat
    10381 gcttcatcct atgtgttgaa acttcattgc tccttgtctg ttgaccgtta ttcccaatcc
    10441 gttcccacaa ccttcggctt gggtttgcgc ctgcgacggc ggcgtttttt cgcctgtcct
    10501 tcgttttttt cgtgccgggc ggcggcctcg tttttggtca tttccgtccg ctgttccgcc
    10561 gatgccgtct gaaacgccgt ccaccactcg gcaagtgcgc ggtccgcatt gccggtttcg
    10621 gcgcgcaaga gcaggaaatc ataggcggca cggaaacgcg cctgtgcaaa cagtttgtgc
    10681 ggacgtgcgc ctttgcggtt ttcaaactgc ggctggaaca tccaaatttc acgcatcgtg
    10741 gcggaaaagc gttgcggcac gccccaaccg cgttcgacgg tttcgcgcat cgtattgatt
    10801 gcatcggaca gggcgggcgt aggtttcaaa ccctgttgca gattgctttt ccaatggcgg
    10861 tccaactctg gccacatcag ggccgccagt acgaaaccga ccgaaaccga tttgtcggca
    10921 cgcagccgct catcggtatt tttcagggca agcgccgtca tttttccggc gatgccgtct
    10981 gaaacgcgca aagcattgag cagcggatgg atgtccggta tgtcaaatcc gttcaaacgt
    11041 ttcagacact cgcgcgcgtg ccctgaaaac agcagtttca tgatttcgtc gaacagcctt
    11101 gccacaggtt cgtgcttcag acggcatatc gattcggcaa tcggtgcggc ggtttcttcc
    11161 gacagctcaa agcccaattt gcccgacagg cggatggcgc gcaaaatcct gacagggtct
    11221 tcctgatagc gttcggcggc attaccaatc ataaccagcc tgcgggcggc aacatcggca
    11281 atcccgttgt ggaaatccaa aatctcttct ttttcaggat cgtaatacaa ggcattgcag
    11341 gtaaaatcgc gccgcatcgc atcttcttcg atgctgccgt aggtgttgtc cttcatgatt
    11401 ctgccgtgcg cgttctgatg aaccttcgca ccgccgcgaa acgtcgttac ttcgataatc
    11461 tccgcaccgt tcatcacatg gacaatctga aaacgcctgc cgatgatgcg gctgcggcgg
    11521 aagagtttgt gcacctgttc gggcgtggca tcagttgcga catcgaaatc tttgggttcg
    11581 atgccgagca gcaggtctct gaccgcaccg ccgaccacat aagcctgaaa ccccgcccct
    11641 ttcaggcggc gtatgacgtt ttcggcggca aagctcaaca tttcggcact gatgttgtgt
    11701 ctttcggcag gaatgaccgt tttactttcc gcttttttac tgctccgacc ggaaggcagc
    11761 atcttattca accatttttt cagcataaat attctgaccc gaagagcggc atctccacgg
    11821 gcaaccgtgt tcagactgca tcaggcggta cgattccaca agatgccgaa aaccataagc
    11881 aaaaggcgta gaaactacgc cgtccgaatc acgccgccct cgcggcggca acgcgtcatt
    11941 ataacagatt gccccttaaa aaatcagacc ctgcttttgt ggcggagtct gaaatttgat
    12001 gcaccttata tattgcgtcc ctaagaaggg atgattaaca aaaattaacg ccctttactt
    12061 tcttttccga ttgggaacat tcccgtcgta caaacaagca aatgaaggaa cagaaatcac
    12121 attcaaacgg cgttccgccg cctctacgcc gcttcccagt agtcgatata aagctgcaat
    12181 tcgaggttgt cgcgccattc gttggcaacg gggcggtaca ccgtgcggat gtattcggga
    12241 atgtcttcgc tgcaacgcca gaacatcgct tcaaattcgc agccgtcttt ttgcaaccac
    12301 gcttttttgt gtttgccttc cgcgcccaaa ggctgctggc gaacgacgtg gaactcgtcg
    12361 gtaaagctcg gcggcgcaaa gccctgcccc caaacgtggc aggcgaggtt ttgcgcctgt
    12421 tccaacgtga tgtcgcaggc gggcaggctg ccgtcagtaa tataggtttg cgacaaatcg
    12481 tcttcgcaca ccatctcacg cacggcttct tcaaaggccg tctgaaacgc gggaatattg
    12541 tgttcaagta tgcttaaacc cgccgccatc gcgtgtccgc cgaatttcaa aatcaaatcg
    12601 ggatggcgtt tggacaccaa gtccaaagca tcgcgcaagt gcagattggg aatggagcgt
    12661 cccgaaccgc gcacttcgcc gttgtccgcc ggcgcaaaca cgatggtcgg acggtaaaaa
    12721 cggtctttga ggcggctggc aacaatgccg accacgcctt gatggaagtc gtcgcgatac
    12781 gccaccaaag tcgtctgacc tgaaggcagg gtttcgggaa aggcgttcaa cgcgtctcgc
    12841 agcatagact gctcgatttc gcggcgttcg atattgaggt tgtttaaccg agccgccagt
    12901 tcctgcgctt cggaatcatc tcgcgccaac aggcaggcga tgccgaccga catatcgtcc
    12961 agccgtccgg cggcgttgat gcgcgggccc aacgcaaagc ccatatcaaa cggctgagcc
    13021 ttgcgccaat cgcgccgcgc cacttcaaac aaggcgcgga tgccggggcg cattttgccc
    13081 gaacgcatcc gtttcaaacc ttgcgacacg aggatgcggt tgttgtggtc gagggggacg
    13141 acatcggcga cggtaccgag tgcgaccaaa tccaaaaggc cgcccagatt cggctctttg
    13201 atgccgtctg aaaaataatt gcggcggcgc agttcggcac gcaacgccgt caacacataa
    13261 aaaatcacgc ccacgcccgc caagcttttg cttggaaaac cgcagccttt ttggttcgga
    13321 ttgacgatga tgcagtcggg tacggtatcg gctggcaaat ggtggtcggt aacgatgaca
    13381 tccaaaccca gagcctgcgc gcgcgccacg cctgcaatac tggcaatgcc gttatccacc
    13441 gtaatcagca aatccacgcc ttgcgcggcg gcgatttcgg cgagttcggg cgttaagccg
    13501 tagccgtgct caaagcggtt gggcacgagg aaatccactt tcgcgcccat cgccgccaaa
    13561 ccgtccaaac cgacggcaca cgcggtcgcg ccgtcggcat catagtcggc gacaatcagg
    13621 attttttcct tgcgttcaac cgcatccgcc aaacggccgg cggcggcttc gcaattcgtc
    13681 agcgattgat aaggcaggag ggaagcgagt ttgtcgtcca actcggcagg actttgcaca
    13741 ccgcgcgagg cacaaagccg ggcgattaaa ggatcggcgc cggcggcgag caaatgattg
    13801 aaaacgtcgg tattgacggg gcgggtttgg attttgactg acatagcgcg gggttttaca
    13861 gtgcgggtgt gcttgaaatg ccgtctgaag gcttttcaga cggcattgcc gttcatttgt
    13921 ctgaggaatt cgcggctgca atcagagcca taggcaagac cagcaccgcg gccgccgcat
    13981 ccaatatcaa caaggggggc gtatataaga tatttccaaa cagcttggat ttgtcggtat
    14041 gtttttcagt aaccgtataa taaatatcgg cgggcacact ttgctcaaaa tgataatcgg
    14101 cgttcagttt ttgcggcgtg gcgtagtatt tgcctttggc ggatacgcag cgcgtgtaaa
    14161 tggtccgatt gtcgagtttg accgctttaa actcaagctg tttcagcttg gcgatgtcgt
    14221 caggtctgcc ggtatcatag cgcaggcaaa gacctccggt actgaaattc tggctgccgg
    14281 gcgcttcgaa tttgaccggc agggcttggt ggcgggcata gctcggggta tcctcaacta
    14341 tttggaaggg cttgtccaac ccggccttca aaaggcccgt cagcttcgcc gaatcttcgg
    14401 gattgacggc gaaccagtat ttcccgccca tcatcaccag gctgcccttt tccaattggg
    14461 cattgtcttc ggcaaccaca ccgaaggcgc ggatttggtc tttgtcaacg tgtttgcggg
    14521 taattgtttg gctgaccggg ttgttcatcc cccgcaacat catcgtacag ccgttcagta
    14581 tcaaggttgc cgccaaaacg gcggcggtcg ttttacggaa caacatttta tcttccaatc
    14641 taaataacgg cggatgccgt ctgaaaatat gcctaaattt ccgcgccccc gttttccatc
    14701 acggttttca attcttgaaa cagggcgcgg aagtttttcg gcggtttgtc ctgttcctgt
    14761 tcttttttgg tgttgcggac gagcgtcctc agcctgcccg cgtcggcgtt tggaaaatcg
    14821 gacataaact gcgtcagcgc gccctcgtct gccaacaacc gtacacgcgc ctgttccacg
    14881 cgttgcaaaa aggcgttgtg cgccgcatcg tcgccgcgca gcttggcaag gaacgcctcg
    14941 atgggcgcgg gatcggtatc gcgcatcaga cgtccgataa attgcgcctg acgtttgagc
    15001 gcgccgttgg acgtaatttt tttataggta acgacggcct cgtacaaatc ttcatccaaa
    15061 ccgatttttt tcagcgtgtc gtttgagagc ctggtcagtt ccatacccaa atcctgcaaa
    15121 ccgttcatct gctttttcat ttgggttttg ctgatccact cgtcttcttg ttcaaacatc
    15181 ttgattctcg aattttttcc aatagcgtat tttaacagaa tgccgccgcg ttcagacggc
    15241 tttttgcacc gcacgccctg caagcggcac gcttttgccc ctgccgcccg aaacggttaa
    15301 aatgccgcaa cgctccacca agaaagattg ttatgctgtt caaccacacc gcttccgaac
    15361 tcctcgacct ctgccgccgc acgctcgact tggcaaaagc agcgggcgcg accgccgccg
    15421 aagccgactt cagcgaatcg ctcggacaaa gcgtcagcgt gcgcttgggc gaaatcgaac
    15481 aaatcgagtt ccagcaggac aaatcgctgg acattaccgt ttacgtcggc aaacgcaaag
    15541 gccgcgccgg caccgccgac ttctccgaac aagccctgcg cgacaccgtc cgcgccgccg
    15601 ccgacatcgc ccgccacacc gccgaagacg gttgcgccgg acttgccgac cccgagttga
    15661 tggcgcaaca catcggcgac cccgaccttt accacgaatg ggatttggat acggaagccg
    15721 ccgtcggctt ggcaaaacaa tgcgaacagg cagcgttgga cacggacagc cgcatcgaaa
    15781 actccgaagg cgcggcggtg caaaccggac actaccaata cgtttacggc aacacccacg
    15841 gttttgccgc acaccggcaa agcacgcacc acagcatttc ctgcagcgtc gttgccgccg
    15901 acgaaaacgg aatgcagcgc gactactggt acgattccgc ctgccgccat ccggatatgg
    15961 acagccccga aaccatcggt caaaccgccg cccgccgcac tttgcgccga ctcggcagcc
    16021 gcagcatccc gacaggcagc taccccgtcc tcttcgatac caccgtttcg ggcgggctga
    16081 tcggacacct cgtcggcaca ctctccggcg gcgcgctcta ccgccaaagc agtttcctga
    16141 tcgacagcat cggcaaaaaa gtcctgcccg atttcctcaa cctgcgcgaa gaaccgcaca
    16201 tcccccgctc tttccgcagc agctattttg atgcggaagg tgttgccacc gcgccgcgct
    16261 tcgtgattca aaacggcatt gtcgaaggct atttcctcgg cagttacagc gcgcgcaaac
    16321 tcggaatgca gaccacgggc aacgccggcg gcgcgcacaa cctgtatttg aaccatacgc
    16381 acgaaacgca atccgacctg ctgaaagaaa tgggcacggg attgctggta accgaattga
    16441 tgggacaggg tgcgaacacc atcaccggcg actattcgcg cggcgcggcg ggtttttggg
    16501 tggaaaacgg cgtgattgcc taccccgtcc acgagattac cgttgccgga cgcttgcagg
    16561 atatgtaccg cgacatcgtc ggcgtggcgg atgacgcttt gcggcgttcg tccaacaaaa
    16621 tcggttcgat tctgattgcg gggatgacgg ttgccggaag ctgaacgccc tgccgatgcc
    16681 gtctgaagac gaaggcggcg cgttttgccc cgtgcctgcc cgtttgccgt tttatgccgc
    16741 accgcctttt accggcgaca ttgcacattt ttttcatcag cactgatttt cccttatccg
    16801 tcaaaatact aagccacttg cccacaacag ggtacacaca agttcaggat aaggcgggcg
    16861 gggattgggg acttttccgc catcggcaaa acggcagaca aaaacggcgg gacattgcct
    16921 gtcccgccgt tttcaaatat acatttgacc gtacatcagg aattaagctg cctgaatgtt
    16981 ggcggcctgt ttgcctttag ggccggtggt tacgtcgaaa gagacgcgtt ggccttcttt
    17041 cagggttttg aaaccttcca tattgatcgc tgagaagtgt gcgaacaaat cttcgccgcc
    17101 ttcgtcagga gtgatgaaac caaaaccttt agcgtcgtta aaccatttta cgataccggt
    17161 tgccattaga aacttcctat acttaaaaat taacaaaatc agcaaaataa ggcatacagc
    17221 aaacttaaac gttcagcgtt tctacccagc tttatatact tggtcgagtg ccgacttttg
    17281 cttaaccgtc tttttacatt tgttaagaca aaacgtcaag ctatgttttc aaaaaagtgg
    17341 gaaaataggc gaaagtaccg aaaatacaac aaaaatgccg tctgaacgcg gctttcaaat
    17401 gggacacgct atgaccgcgc aacaccaatc cgacacgctt ttgcaccgcc tgaacaccct
    17461 gccgccgaaa cgttacggcg tatttttatt gaacgacgat tacaccacga tggaatttgt
    17521 cgttgaaatc ctgaccgaaa tctttatgct cgggcaggaa caggcggtag cggtaatgct
    17581 ctcggttcat cacgaaggca aaggcctgtg cggcacttac acgcgcgata tcgcccaaac
    17641 caaacagcaa caagtcatgc agcgggcaaa agccgaagga catccgctgc aatgtattgt
    17701 cgaggagatt taatatgctt gcccctgaat tggaacagat tttgcagcag ctttaccgcg
    17761 aggcgcgtaa ggctcattat gaatttatca gcctcgagca tctgcttttg gtactcatcg
    17821 aagaagatgc cgccgtgccg aacgtcttaa aactctgcgg tgcggattta aaagcggtgt
    17881 ccgaacaact cgccgccagc gttgccgaaa acacccccct gattcccgac caccttttag
    17941 acacggtcga aacccggccc acgctcggct tccaacgcgt catccagcgg gcgatggtgc
    18001 atacccaatc ggcaggcaaa ggcttggccg agcctttgga cgttttggtc gcgctgatga
    18061 gcgaaaccga cagccacgcc gtctatttcc tcaagctgca atcggttacg cgttttgaag
    18121 ttttgcgctg tattgcccac ggctctcccg atgaagatga agacgatggc aactattttt
    18181 cagacggcat ggacgacgat aatgagaacc gcaccaaacc gggcaaaaac cctttatcgg
    18241 cgtacaccgt caacctcaac gccgaagtca aagccggccg tatcgatcct ttgattggtc
    18301 gcaaacacga aatggaacgg ctggtgcaaa tcctgtgccg ccgccgcaaa aacaatccgc
    18361 ttttggtcgg cgaagcaggc gtgggcaaaa ccgcgctggc ggacggtttg gcacatcaaa
    18421 tcgtcaacga cgacattccc gacgcgctca aagaggccga agtgtacgcg ctggatatgg
    18481 gttcgctttt ggcgggcaca aaataccgcg gcgactttga agcgcgggtc aaatccgtct
    18541 tgaaacagct cgaaaaaatc ccgcacgcca ttttattcat cgacgaaatc cacaccatca
    18601 tcggcgcggg cagcaccggc ggcggcacga tggacgcgtc caacctgctc aaacccgcat
    18661 tggcgaaagg ttcattgcgc tgtatcggcg caaccactta cgacgaatac cgtactattt
    18721 ttgacaaaga ccacgcccta agccgccgct tccagaaaat cgacgtggtc gaacccaccg
    18781 ttgccgaaac cgttcaaatc ctgcgcggct tgaaaccgat gttcgaagcc ttccaccaag
    18841 tccgctacac gcaaggcgca ctcgaagccg ccgccgaact ctccgcccgc tacatcaacg
    18901 agcgcttcct gcccgacaaa gccatcgacg taatggacga agcaggcgcg gcgcaacgga
    18961 ttctgcccaa atccaaacag aaaaaagtca tcggcaaagc gcaaatcgaa accgtcatcg
    19021 ccaaagtcgc gcggattccc gaaaagaccg tgtcgcacga cgacaaacaa gtgttgcaat
    19081 tcctcggccg cgatttgaac aatatggttt acgggcagga agacgccatc gacgcgctgg
    19141 tttccgccgt caaaatgtcg cgttcgggct tggggctgcc cgacaaaccg ataggcagct
    19201 tcctcttctc cggccctacc ggcgtcggca aaaccgaagt cgccaaacag cttgcctact
    19261 caatgggcgt accgctgcaa cgctttgata tgtccgaata catggaacgc cacgccgtat
    19321 cgcgactcat cggcgcgcca ccgggctacg tcggctttga acaaggcggc cttttgaccg
    19381 aagccgtcaa caagcagcct cattgcgtgt tgctcttgga cgaaatcgaa aaagcccatc
    19441 cagatatttt caacgtcctc ctgcaagtca tggacgcagg caaactgacc gataacaacg
    19501 gtaagagcgc cgatttccgc aacgtcatcc tgatcatgac caccaacgca ggcgcagaaa
    19561 gcctcagccg acccagccta ggctttaccg ccaaacgcga gcgcggcgac gaaatgcagg
    19621 caatcaacaa actcttcacg cccgagttcc gcaaccgctt ggacgcgatc atcccgtttg
    19681 cacctttgtc cgaacccatc atcgtcaaag tcgtggacaa attcctgctc cagctcgaac
    19741 accggctcct cgacaaaaaa gtcgaagccg aattcacacc ggcattgcgt aaatacttgg
    19801 cagaaaaagg tttcgacccg caaatgggcg cgcgcccgat gcaccgcctg attcaggaaa
    19861 aaatccgcaa accgctcgcc gacgaactcc tgttcggcaa actcgccgac ggcggctttg
    19921 tgcggataga ctgggatgcg gcgaaagaag aagccgtatt gaagtttaag aaaagcaagg
    19981 tcaaaataaa aaccgcctcc gcataaaatc aaaaatgccg tctgaaaggt tcagacggca
    20041 ttttgtcaaa tatcggcccc tattccggca aacttctcca caaacgccgc gattttttgg
    20101 aagacgcttt gtttcttcgt cagatattgc gggttgagcg gactcatttt cggcagggtt
    20161 tcggtcagtt ccgtgccgtt ttcgctggca tagccgcgtt tgagcgagcc ggtcaaatag
    20221 cgtttggcgg cggtttcgtt cagaccttcg gcggcaatca atcctgccgc ttcgtgccgc
    20281 atcacctctt gcgcgtaggt gtaaaaggtt tccagtatgg cgggaacgtc gggcacttta
    20341 tccaaatccg tatcgttgat gaaatccaca atcaggccct ctttggcgcg gtggccgatg
    20401 ctggcgcgga tgatgcggcg gatttcttcc accagctccg ctttgccttt gatttttttg
    20461 tgatgctcga aaaccagttg caggatgtaa tccagattga tttcctgcga tttgagcaaa
    20521 tccacctcaa aaaccacatc gtcccagtcg atttttgact gttctttctg ttcgcctgct
    20581 ttttggcggc gcagccagtc tcggatgtcg ttataggcgg aacggtagtc ctgcaccgcc
    20641 ctttcagacg gcatcggcac tttccgcatt tcctgcacgt cttcgtcgct caggtagtat
    20701 ttttcctgaa acgccttcat cgcatcttcg tccgccgcgt ccacattctg caattcgcgc
    20761 agcgcggcaa attcatcgta gttctgcaat acgttttccg cccgcaggta ttcgccgaag
    20821 agtttggcaa aatctttttt gtctttttcc gtttcgattt tgtcgggatc ggggaaacgc
    20881 tcgtgcaatt cttttgctac atccagataa ccgcgccgtg cttcgccggt ctggctgtcg
    20941 gtatagccgt tcatgtattc ttcgtagctt ttttccagca ctatattttt ggtgtttttg
    21001 tcgccgaaga gcgtgatcgc atcaatggtt gcctgctcca agtcgcggaa gcagacaatg
    21061 ttgccgaagg ttttggtagc atcgtaaatg cggttggtgc gagaaaacgc ctgcatcagg
    21121 ccgtgatagc gcaggttttt atcgacgaac agcgtattca gcgtcggtgc gtcaaaaccc
    21181 gtcaaaaaca tgccgaccac aatcagcaag tccacttccc ggtttttcac ccgttttgcc
    21241 aaatcgcggt agtagttctg aaaggctttg ctgtccgtgc cgaaattggt tttgaaacag
    21301 gcgttgtaat cgttgatggc agcctgcaaa aattctttcg cgctgctgtc catcgcttcc
    21361 ggttcaaagg tctcgtcaac aatttcaccg acggcgtttt gctcttcgtt ggcggcaaag
    21421 gaaaaaatgg tggctgtttt caacgggtgc aggctgcctg cctgttgtgt tttgaacgct
    21481 tcgtaatagc acttcgccgc atccacgctg ctgacggcaa acatggcgtt gaagcctttg
    21541 ccgcccgcat tcaagcggtg cgttttctgc ctgaaccggc tcaggatata ttgcgtgatt
    21601 tcgcggatgc gttcggggtg cagcagggct tggcggtttt cggcggcact cagttccttc
    21661 tcgtcctgtt ccgcttccac ggctttgaac tgcgggcgca catcgttgta atccactttg
    21721 aatttcaata ctttttcatc gcggatggca tcggtaatca cataagaatg cagctcccgc
    21781 ccgaacacgc ccgccgtggt ttccgcgccc aaagcgtttt cgggaaaaat cggcgtgccg
    21841 gtaaagccga actggcagaa ttttttaaat ttctttttca ggtttttttg cgcttcgccg
    21901 aattgcgagc ggtggcattc gtcgaaaata aatacaacct gccggtgata aaccggcaga
    21961 ttatcttcac ttttcatcag gttgttcagc ttttggatgg tggtaacgat gattttgttg
    22021 ccgtcttttt ccaaattgcg tttcaagccc gccgtacttt ccgagccgtt tacgctgtcg
    22081 ggcgaaaaac gttggtattc cttcatcgtc tgatagtcca aatccttcct gtccaccacg
    22141 aagaaaacct tgtcgataaa cgccgattcc gtcgccagac gcgccgcctt aaagccggtc
    22201 agcgttttgc cgctgcccgt ggtatgccaa acatagccgc cgctttccgg cccgctccaa
    22261 ttcttcgcct gcgccgagct gttgattttc cacaaaatgc gttcggcggc ggcaatctgg
    22321 tacggccgca taatcagcag cgtatcattc gcatcgaaca cgctgtaatg cagcaaaacg
    22381 ctcagcaata cgcttttctg caggaacgtg gcggtaaagt ctttcaaatc cttaatcgga
    22441 tgattgtccg accgcgccca attcatcgtg aaatcgaagc tgtttttgtc gcgcttggtg
    22501 gtgttggcga aataacgcgt gtccgtgccg ttggaaatca cgaagatttg caggaatttg
    22561 aacagcgaat tttcgctgtt gaagctctct ttgctgtaac ggtgcacctg attgaatgcc
    22621 tcgcgcacca ccacgccgcg ctttttcaat tcaatctgca ccagcggcag gccgtttacc
    22681 aacacggtaa cgtcataacg gtttgcatgc gtgcccgtct gctcaaactg gttgataagc
    22741 tgcacatggt tgcgggcaag gtttttcttg tccagcagat aaatgttttt cagacggccg
    22801 tcatcaaaag cgaaatcgta aatatggtcg tcgtggattt tgcgggtttt atcggtaatg
    22861 ttttcagccg gcctgtccag atattccgtc aaaaaccgcg cccattcgcc gtctgaaaac
    22921 gccacatcgt tcagccgctg caactgcgcg cgcaggtttt ccagaagcct gctttggctg
    22981 ttcaaatcct tgcggtattc gtacccctga ttctgcaaat cggcgattaa ctccgcttcc
    23041 aaccggtttt ccgattggta gctgcccgac tgttcgattt tttcatattg gtcgagcacg
    23101 atgaaattcg gcgtttcggc gatgggtttg gtttcgaggt tcatgggtcg tcctttgcgg
    23161 tgtcgggttt tatagtgaat taacaaaaac cggtactgcg ttggctcgcc ttagctcaaa
    23221 gagaacgatt ccctaaggcg cccaagcacc aagtgaatcg gttccgtact atttgtactg
    23281 tctgcggctc gccgccttgt cctgattttt gttaattcac tataaacggt ttgatgttcg
    23341 gattgggctg cctgaaaaag tgcaggctgc tttttcaagc agccttgggg aaggcaagca
    23401 gctgttcgcg gtaatattcg tattgtttgc ggcgcagggc gatttcgtgc ggcaggcctt
    23461 cgctgacgga gtgggtcagg gtatcgaatt tgcctaggat ggcgacgatt ttttcctgtt
    23521 cggggagcgg ggggatgggg attttatatt gcataatcgc tgctttgctg ccacgcggca
    23581 tttttgcacc tttggcgtgc ttcatattaa aagcaaaaaa tttatcatcg gccaatacct
    23641 gatacagata ttttggatta accttttcat ctgttacccg gataaccaaa acatcaccgt
    23701 ttgttccgcc cgtgcagtct gcctgccaga tttttttcaa atacggacgg atatttccaa
    23761 tcaaaatgtc attgacaata tattctgtca tttttccttc gcttggaaca taaccggata
    23821 actttttacc ttctctgttt tgtaagagat tatccacgcc aacgtaatta tgttcgttca
    23881 gtttatccga acaaatacgg ttttttgaat attcggcaac ctcccccaac gtcttccaaa
    23941 ccacattttt cagacggcat tgatagccgt cagctatccc ccccgatttg attgtcaaaa
    24001 tctaaaagta agtcgcggta ataccggtat tggcgtttgc gcagggctaa ttccgcttcc
    24061 agcgtagctt ccagcgtagc ttccagctcg gtgaatttgt caagtatttt tacaattttt
    24121 tgttgggttt ccaatgatgg gatggggatt ttatagttat ctgtatctgg tgttgctatt
    24181 tgtggcattt gcatttttga tccgatatta cgaaaatagt tttcttgtgt ttttaagaaa
    24241 taataaacat attttatata aatatgttta ttaactgaat gataagacca catttcattt
    24301 ttatgcgaaa atggtttatc gtaatattcg aattcaataa tacctcttga tttaacgata
    24361 atagaaggct ctctatggat atccttatca ggaacatcat caaagtcaac taaatgcaaa
    24421 agtttttcct cctgcaaaaa tctttaacgg cgcattatct ttatgcattt ctttcatttg
    24481 tccagcagtt atcttagttc cttttgtacg aactaaaacc tcccccaatg gcttccactc
    24541 caccggtgcc gtctgaatca tctcaatcaa ttttttcgct ttgctttgca tatccatggt
    24601 tgttttccgt ttccttcccg ctgcggcggg atatggggcg gttgcggggc gggtttcagc
    24661 ccgctatgtc cgtttgccgt tatttctgcg ggctgaagcc cgtcctgctt tcaggctgcc
    24721 tgaaatcgtg tgcaggctgc tttttcaggt agcctgaatg cggttatttt ttgccgtttt
    24781 gatgctgttc ggttgcggca agccttttct gttcttcgtt ctcaagctgt ttgatgcttt
    24841 tctcgggtac gggctggttt tcaggcatga tgccgcccag ttcttcaatg gtttggcgca
    24901 ctttttgtcc gacttcgaaa tgcacccggt ttgcctgtgt tttgccttgg atgtttttgc
    24961 ggcgtagttt ttcttcggtt tgcgtggcac gaaacagatt ggcagccggt tcgctggcgt
    25021 tcatgtggtc taggattctc tggcttttct tcaagccttt gcggctgtgt atgccctgct
    25081 tgtccagtcc tccgtatagc ccccgatagc cgtggttttg gaagacggcg tactcaacgg
    25141 gtttttctac gcctgcatcc ttggctgcgg cggcaaggtc ggtattgtgt tcgcgcagct
    25201 gcctgcaaag cagcaggcgt tgtttgtctt cgcccaaact cctgaatgct gcctcgtctt
    25261 gcagctcttg gcggcgggct tgcacggcaa agtaggtttg ccctgcggca atgacgcttt
    25321 tggacggatc gccgttttgc acaatcaggt agcaggcata gcgggaaagg cggtaatccg
    25381 ccacttcgcg ctgcccgcct ttcccaaggg ctatcatttt gttggtttca acaaaatgat
    25441 gatttttgtc atttcctgat gtttcgcaag cggttatggc tttatcaatg gcccgttgga
    25501 aattgcgcca ttcggaatat tccaagattt gctgcaaggt gcgcgccgac caatattcat
    25561 tgcctgtttc gtcgatgtgt ttggcgtttt caaaagcgtt attttctgtt gtcatgtatt
    25621 ttccccgaaa atttcttgat ttgaacttgt aaggattgct tacaggttgc cgcccgtatt
    25681 ttcagacggc ctctttccca ttcagttgtt gcaaaatatg caacaactgc ctaggcctca
    25741 atctctgcaa tcacttcgtc aatttcacgc cgcagccgtt cgattttggc gacggtttcg
    25801 ctgatttcgg cgttaagctg tctgatgtcg atgacctcgc gggtgtcttc ggcttcgaca
    25861 tagctgctga cggcgaggtt gtagccgttg tctttgacgg tttgctgggc ggcgttttgg
    25921 gcgatatgcg gcacatcggc tttgtcggcg aagagtttga cgatttcggc aatgtgttct
    25981 tcggttaaga cgttgttgtt ggtttctttt ttaaagaagc cgcctgcatc gatgaattgg
    26041 atgtcggtat tgtctttgtg tttggacaaa accaggatat tgacggcgat gcaggtgccg
    26101 taaaagagat tgggcgcaag ggcaatcacg gtttccacat agttgccctc caccagatat
    26161 tggcggattt tctgctctgc gccgccgcga tagaaaatgc cggggaatga gacgatggcg
    26221 gcgcggcctc tgccggaaag gtagttcagt gcgtgcagga taaaggcaaa atcggctttg
    26281 gatttcggtg cgagtacgcc tgcgggggca aagcggtcgt cgttgatcaa ggtggggtcg
    26341 tcgctgccta tccagtcgat ggaatagggc ggattggaga cgacggcatc aaagggtttg
    26401 ctgtctttga gtttggggtt ggtcagcgtg tcgcccaatt cgatgtggaa tttgttgtaa
    26461 ttgacgttgt gcagaaacat attcatgcgg gcgaggttgt aggtggtgtg gttgatttcc
    26521 tgcccgaaga agccttcttc gatgatgtgt tcgtcaaact gttttttcgc ctgcaagagc
    26581 aggctgcccg agccgcaggc ggggtcgtag attttgttga ctttctcctg cccgtgcacc
    26641 gccagccgcg caatcagctt ggagacgctt tgcggggtga aaaattcgcc gccggatttg
    26701 cctgcgttgg cggcgtagtt ggaaatcagg tattcgtagg catcaccgaa aaggtcgatg
    26761 cggtggtctt caaaattgcc gaaatcgagt tccgccacgc ctttaaggac ggcggcaagg
    26821 cgtttgtttt tgtcggcaac ggtgctgccg agccggctgc tggtggtgtc gaagtcgtca
    26881 aacaagcctt tgatgccttg ttcggttgtt cggacgggta gccggaggcg gagctttcaa
    26941 tcgcggtaaa gatttctttc agcttggtgt tgagctcttc gttttgatgg gcttcggcgg
    27001 caatattgca aaaaagctgg ccggggtaga tgaaatagcc tttgactttg acggcatcgt
    27061 ctttgatttc gggcgtgatg atgctgtccg gcatagcggc gtaatcaatg ctgctgtcgc
    27121 cggcctgcat atagtcggtg aagttttcgc tgataaagcg gtagaaaagt gtgccgagaa
    27181 cgtattgttt aaagtcccag ccatccaccg cgccgcgtac ttcgtcggcg attttccaaa
    27241 tttggcggtg cagttgggcg cgttgttgca tttcggtcat catcgaaatc catatataaa
    27301 gttaaacaaa tcaaaatcgc ctgatatttt cagacgattt ttttacgggc attcaaatta
    27361 agccaaattt tccagcatcc atttgacgta gcggttgaca cctgctttga catcgaaaaa
    27421 ttcttcctta tatccggctt cgcgcaattt ggtgatgtcg gcttgggtga agccctggta
    27481 tttgcctttg agcgcgtcgg gaaaggggat atagcggata agttcttctt ctaccaactc
    27541 tttcaagctc aattcagatt tgccttcggc ggcgcggcag gcgttgacgg cggcggcggc
    27601 gagttcgttg aactgttggc tgcggccggt gccgaggttg tagatgccgg aaagttcggg
    27661 atggtcgaag aagtagaggt tgattttggc gacgtcttcg acgctgacga agtcgcgggt
    27721 ttgttcgccg ttgccgtagc cgtcgttgct gccgaacagg ttgacgtaac cgtgttcgcg
    27781 gtattggtgg aaatggtgga aggcgacgga tgccatgcgg cctttgtgtt gttcgtgttg
    27841 tccgtaaacg ttgaagtagc ggaagccgac gacttgggcg gtgagacctt ctttcatgcg
    27901 gcggcgcaat acttggtcaa acaggaattt ggagtagccg tacacattaa gcggtttttc
    27961 gagttcgcgc tcttcgcgga agatttcgcc tttgccgtaa accgccgcac tggaggcata
    28021 aaggaagggg atgcgttcgt cctgacacca gtccaacaaa tccagcgtgt actggtagtt
    28081 gttttccatc atatacaaac cgtcgtggtt catcgtatcg gaacacgcgc cttgatggaa
    28141 aacggcttcg atgttttgat aaggtaaaat gtgttccctc acttggcgga taaattcgtg
    28201 tttgtcgagg tagtgggcga tttcgcactc ggcaaggttt ttgaattttt cgccttttgt
    28261 cagattatcg acggcgacga tgtcggtaat gccgcgttgg ttgagggctt tgacgatgtt
    28321 gctgccgata aagccggccg cgcctgttac gatgatggtc atattcggtt tcctttgttt
    28381 tgagggtttc agacggcatt ggatggtcat gccgtctgaa aagatttatt gcccgtccag
    28441 tgcttcaacc aactctgcaa acgagcaaac cgccgtaccg agtttcgcca cgacaacccc
    28501 ggccgcagta ttggcaaggt acatggcttc gggcatggtg cagcctgccg ccagccccaa
    28561 gcccattccg gcaatgacgg tgtcgcctgc gccggacacg tcgtaaactt cttgggcgcg
    28621 ggtgggctgg taaatgggtt cgccttcgct gaacagggtc atgccctctt cgctccgggt
    28681 cagcaaaacg gcggtcaagt cgaggtggcg gcgcaggttt tgcgcttttt cggtcagatc
    28741 gccttcgttt ttccaactgc caaccacttc tttcaattcg gcgcagttgg gcgtaatcag
    28801 agttgcgccg acatattttt cgtaatcgtc gcctttgggg tcgattaaga cggttttgcc
    28861 gacatgtttc gcccaatcga tcatatcgga gatatgcgac agaccgcctt tgccgtagtc
    28921 cgaaaaaatg attgcgtcgt attcgggcaa gatttcgcgg tatttccgct tgatttgttc
    28981 caacacttcg cggttgggat gttcttcaaa atcaaggcgg ataagctgct ggttgcgggc
    29041 gacgacgcgc agtttgacgg tggtggcgat ttgtttgtcg cgcatcagat aggaggcgac
    29101 gccgtcctgc accatcagcg cgtcgagcgc ggcggcggct tcgtcgtcgc cggttacgga
    29161 caacagccct gccctgccgc ccaacgaagc gatgttgcgc gcaacgttcg ccgcgccgcc
    29221 cgcccgttgg tcgattcgtc cgatttttgc caccggcacg ggtgcttcgg gcgaaattcg
    29281 ggacacgtcg ccaaaccaat agcggtcgag catcacgtcg ccgacaacaa gaactttggc
    29341 ttgtgcgaaa cgggatttga gggtttcttg ttggaacttg gcgggcatat tgcctccgtt
    29401 gtgttttgtt cagacaacct taacctgtaa gacctcctga aaatcagttt gcttcaaggt
    29461 ttgccacgcg gttcacttcg cgcaatacgg cgaccggatc ggcggctcgg gtaacggggc
    29521 ggcccatcac cagataagtc gaacccgccg ccaaggcttc ggcgggtgtc ataatgcggc
    29581 gttggtcgtc gttgttgccg gcaacgtcca agcggatacc cggtgtaacc aagacaaaat
    29641 cccgtcccaa ttcgcggcgc agcggcgcgg cttcttgggc ggaacagacc acgccgtcca
    29701 agcccgaact ttgtgccagt tttgccaaac ggatgacttg ttcttcaggg gcggtgttca
    29761 aaccgatttc cgccaaatcg ctttgctcca tgctggtcaa cacggttacg ccgattaaga
    29821 gcggtttcgt gccgtatccg gcaacggctt cggcggcggc ttccatcata cgtctgccgc
    29881 ccgacgcgtg catatcgacc atccacacgc ccatatcggc ggcgacttta cacgcctgcg
    29941 cgacggtgtg gggaatatcg tggtatttca aatcaagaaa gagtttgaaa ccctgatgaa
    30001 tcaggctttc cgccaaactg cgtcccgtcg cggtaaacag ctctttgccg attttgattt
    30061 ggcacaacgc cggatcaagg ttgcggacga atccgagcgt gtctttttcg ttggcaaaat
    30121 caagggcaac gatgacgggg gtacgttgtt gcggagtttg gaagtcggtg attaaaggat
    30181 tcatggtgtt ttcgtttcgg ttttcagacg acttttatcg tctttttcgc agtacaacgt
    30241 aaattcaaac cgtaggccgg gcattgatgt ccgacctacg cggactcaag cccccgacaa
    30301 actgtttcgg ataaatttga accggtacat cagccgtcat tcccgcgcag gcgggaatcc
    30361 attgataaaa ctcagttcat cagatttaaa cagcgattgc ccaaattcag aaatggattc
    30421 ccgcctgcgc gggaatgacg ccgaatgatt atttttcggt tgcaacacgc tgtttatcaa
    30481 aaacaggtcg tctgaaacct gattttctgt tttcagagtg cgaagcctgc ccgtgggtac
    30541 gaccttttcg atttatttgc ccaatgcgcc caatacttcg gctttcacag cttctactgc
    30601 ttgggtaccg tcgactttga tgtatttcgg cgcgtgttcg ccttccagct tgctgtaaaa
    30661 atcaaccaaa acttcggttt gctcgtggta aacggcaaga cgttttttca cggtttcttc
    30721 tttgtcgtcg tcgcgctgaa tcaaatcttc gccggttacg tcgtctttgc cttcggtttt
    30781 aggcggattg taggtaacgt ggtaagtacg gccggaagcc aaatgcacgc ggcggccgct
    30841 catgcggtcg acaatcacgc tgtcggatac gtcgatttca acgaccgcgt ccaaacccac
    30901 gcctgcttcc accatcgctt cggcttgcgc caatgtacgc gggaaaccgt cgaacaggaa
    30961 accgtttttg cagtcgtctt gcgcgatgcg ttctttgacc atgccgataa tgatgtcgtc
    31021 gcgcaccaaa ccgccttcat cgatgatttt tttcgcttcc aaacccaagg gcgtgcctgc
    31081 cttaatcgcg gcacggagca tgtcgccggt agagatttgc ggaatgccga acgcggcggt
    31141 gatgaattgc gcctgagtgc ctttgcccgc gcccggtgcg cctaaaagta atgctttcat
    31201 gaagtgatcc ttttaaatgt gtttattgga atcaaacaga tggtttacga ttctacacgg
    31261 cttggcgcaa tagttcaaaa accaactgca caaactgcca tttgcaagca aatgttttgc
    31321 atacctttgc cgagcctgat attcacaagg cggcaacttt ttgggacaat ccccaaagtt
    31381 tcaaaacatc acaccctacc aaaaaggaac atccgtgaaa cgcatttttc tgtttttggc
    31441 taccaatatc gctgttttgg tcgtaatcaa cattgttttg gcggttctgg gcatcaacag
    31501 ccggggcggc gcgggcagcc tgttggcgta ttccgccgtc gtcggcttca ctggttcgat
    31561 tatttcgctg ctgatgtcca aatttatcgc caaacaatcg gtcggtgcgg aagtcatcga
    31621 cacgccgcgc accgaagaag aagcctggct tctgaacact gtcgaagccc aagcgcggca
    31681 atggaacctg aaaacgccag aagtcgccat ctaccactcc cccgaaccca atgcctttgc
    31741 cacgggcgca tcgagaaaca gctccctgat cgccgtcagc accggtttgc tcgaccatat
    31801 gacgcgcgac gaagtggaag ccgtgttggc gcacgaaatg gcgcacgtcg gcaacggcga
    31861 catggttacg ctgacgctga ttcaaggcgt ggtcaatacc tttgtcgtgt tcctgtcgcg
    31921 cattattgcc aacctgattg cccgaaacaa cgacggcagc cagtcccagg gaacttattt
    31981 cctagtcagc atggtattcc aaatcctgtt cggcttcctt gccagcctga ttgtcatgtg
    32041 gttcagccgc caacgcgaat accgcgccga cgcgggcgcg gcaaaactgg tcggcgcacc
    32101 gaaaatgatt tccgccctgc aaaggcttaa aggcaacccg gtcgatttgc ccgaagaaat
    32161 gaacgcaatg ggcatcgccg gagatacgcg cgactccctg ctcagcaccc acccttcgct
    32221 ggacaaccga atcgcccgcc tcaaatcgct ttaaaccgat ttgaaacgac aaaaaaccgt
    32281 acgcacaagc agtacggttt tttcgtatat gccgactgaa aacatcaggt ttccataccg
    32341 gcaccaaccg tatccatttc cgcaccggat acgccgcatt gatatatccc cgacaaaccg
    32401 tgtccggaca gaaaatatca gataaaacag cggtaaaaat gccgtctgaa accaattagg
    32461 gcttcagacg gcatttttac tgcaaaacct tatgcgaacg gatgatgcag cacaatcgtt
    32521 tcttcgcggt cggggccggt ggagacgatg gcgaccggcg cgccgcagac ttcttcaatc
    32581 cgtttcaaat atgctttggc attggcaggc aatgcgtcgt agcttttcac gccgacagtc
    32641 gattcgcgcc agccgggcat ggtttcgtaa atcggtttgc aggtttccac cgcatccgaa
    32701 ccgcaaggca ggatgtcggt tttgccgccg cctggcaatt cgtagccgac gcagatattg
    32761 atggtttcaa cgccgtccat tacatcgagt ttggtaatgc acataccgga aatgccgttg
    32821 atttggatgg agcgtttcag ggcggcggca tcaaaccagc cgcagcggcg cgcgcggccg
    32881 gttaccgaac cgaattcgtg tccgcgctcc gccaaacctg cgcctacttc gtcgaacaat
    32941 tcggtcggga acggacccga accgacgcgc gtggtatagg ctttgacgat gcccaaaaca
    33001 taatccagca tttgaggacc tacgcccgcg cctgccgaag ccgcgccggc gagacagttg
    33061 gacgaggtaa cgaaggggta agtgccgtag tcgatgtcca acaacgcacc ttgcgcgcct
    33121 tcaaacagca gtttttcgcc gtttttgttt ttttcgttca acacgcggga cacgtcggca
    33181 atcatcggcg caatgcgcgg cgcgactttt tcgataaccg ccatcacgtc ttccgcttta
    33241 accggtccgg cgttgtgcag gtattggagt tggacgttgt aataggcaag gacggcatcc
    33301 agtttttcac gcagtttttc aggatgcagc aaatcggcgg cgcgaatggc gcggcgtgcc
    33361 actttgtctt cgtaggcagg gccgatgccg cggccggtcg tgccgatttt gcctttgccg
    33421 cgcgatgctt cgcgggcttg gtcgagcgcg atgtggtaag gcaggatcag cgggcaggtc
    33481 ggcgcgattt tcagacggcc ttcgacgttt ttcacgcctg ccgcgttcaa ctcgtcaatt
    33541 tcgcccaaca gggcttcggg ggaaacgaca acgcccgaac cgatgaagca atccaatcct
    33601 tcgtgcagga taccgctcgg aatcaggcgc aaaatggttt ttttgccgcc gacgaccaag
    33661 gtatggcccg cattgtggcc gccttggaag cgcacgacgc cgccggcttc ttccgccagc
    33721 cagtcaacga ttttaccttt accctcgtcg ccccactgtg cgccgattac tacaacattt
    33781 ttagccatag ccatataacc tatcgatatt aaaaattatt gcggaaaatc cccaaaccgg
    33841 tgcggctttg cgccccgccc cggtttgcct ttcatccgct atgaacgggt atttagcgtt
    33901 tcacgatctg ccaaacgccg tccgtctttt tcagacggcc tgcaagctct tccgaaacat
    33961 tgtgtccgat accgtagtca atcacgacac actgcccttg ttcacgcaag gcttcgaccg
    34021 cttcgcgcgc cgcttctgca tcttccgcat cgaccaacac ggcgggctgc cgttcgacgg
    34081 cgggcaaacg cccgataaag ctgcgtaagt cgaaactgaa tcccgttgcc gggcgcgccc
    34141 tgccgaaata tccgcccaat ccgtcataac gcccgccgcg cgcgaccgcg tcgtggaaat
    34201 cggcggcata ggcggcatac agcaagcccg tgtggtaatt gtcgacgcgc agctcggaca
    34261 agtcgatatg gatttcatta tcggggaatg cgtcgcacac cgcctgcaat tcgtccaacg
    34321 cgccgccgac cgccgacaaa tccggcaacc gtccgcgcgc gtcggacaac acttcacgcc
    34381 cgccgtacag gcgcggcagc aacgagaatg cttttgccca cataccgtcc agcttccaag
    34441 ccttgacctg cgcttcgacc gacccggtat ctttatcctg catcaaggca agcagcgttg
    34501 cggactgccc cgcatccaaa tgcgccgcat cggacaaggc gcggaatatg ccgatatgcc
    34561 ccagcgaaag cagcactttg cccatatcgg caattttcat gcttttcagc ataaggtcta
    34621 tcagctcgat gtcgccacgg atgtcggcaa aaccgtacat ttccgcccct gcctgcaagg
    34681 gttcgcgcat attcggcaga ccgtcgggct gcgcgtgcaa caccggaccg gcataacaca
    34741 accggttaat cccttggttg gcggacaaaa gatgggcatc gatacgcgcc acctgcggcg
    34801 tgatgtcggc gcgtatgccc aactgcctgc cgctgagcct gtccgttacc aaaatggttt
    34861 tcagtgaaag ccccgcatcg atatgcgtca gcagggaatg tgcgtactcc atcagcggag
    34921 gctgtaccag ttcataaccg tgtacgcgga acagtgccaa caactgctcg cgcgcgcttt
    34981 caagctgccg cgcgttcgtg ggcagtacgt cggcgatatg ttccggaagc tgccatgtct
    35041 gcatgagaat ctgccttctg ttttatcctg taaacacaaa agactgcccg atccgcaatc
    35101 agacggcatc tttgccgcaa cgcatcacgc cgctgtcaaa acggcgatgc ttccttccga
    35161 ttcagaatcc gaacagcccc tgtccgacgg ctcaatgccg tcaagtcgtt aaaaccaaac
    35221 tttaccataa aatacacaca atctgaatat acttttatac cccgtatccg ccgtctgacg
    35281 gcacggtatt ttttactata taatgtgcac cattatttct catcacttcc cgctgccgtt
    35341 tttttgtttc agacggcatt tttattgact gaacacttat gctgctcgac ctcgaccgct
    35401 tttccttttc cgtcttcctg aaagaaatcc gcctgctgac cgcccttgcc ctgcccatgc
    35461 tgttggcgca ggtcgcgcag gtgggcatcg gtttcgtcga taccgtgatg gcgggcggtg
    35521 cgggcaagga agatttggcg gcggtggctt tgggcagcag cgcgtttgcc acggtttata
    35581 ttacctttat gggcattatg gcggcgctga acccgatgat tgcccagctt tacggcgcgg
    35641 gtaaaaccgg tgaagcaggc gaaacggggc ggcaggggat ttggttcggg ctgattttgg
    35701 ggattttcgg catgattttg atgtgggcgg cgattacgcc gttccgcaac tggctgactt
    35761 tgagcgatta tgtggaaggc acaatggcgc agtatatgct gttcaccagc ttggcgatgc
    35821 cggcggcaat ggtacaccgc gcactgcacg cctacgcttc cagcctgaac cgcccgcgcc
    35881 tgattatgtt ggtcagcttt gcggcgtttg tgttgaacgt gccgctgaac tatattttcg
    35941 tttacggcaa attcggtatg cccgctttgg gtggcgcagg ttgcggcgtg gcgacaatgg
    36001 cggtgttttg gttcagcgcg ctggcattgt ggatttatat cgccaaggaa aaattcttcc
    36061 gcccgttcgg actgacagcg aaattcggca aaccggattg ggcggtgttc aaacagattt
    36121 ggaaaatcgg cgcgcccatc gggctgtctt attttttgga agccagcgcg ttttcgttta
    36181 tcgtgttttt gattgcgcct ttcggcgagg attatgtggc ggcgcagcag gtcggcatca
    36241 gtttgtcggg gattctctat atgattccgc aaagcgtcgg ctcggcaggg acggtgcgca
    36301 tcggcttttc gcttgggcgg cgcgaatttt cgcgggcgcg ttatatttca ggagtgtcgc
    36361 tggtgtcggg ctgggtgctc gccgtgatta ccgtgctttc cttggtatta ttccgttcgc
    36421 cgctggcaag catgtacaac gatgatccgg cagttttaag catcgcctcc accgtcctgc
    36481 tgttcgccgg cctgttccaa ccggcagact tcacccaatg tatcgcgtcc tatgccctgc
    36541 gcggctacaa agtcaccaag gtgccgatgt tcatccacgc cgccgccttc tggggctgcg
    36601 gcctgctgcc gggctatctg ctcgcctacc gtttcgatat gggcatttac ggcttctgga
    36661 cggcattgat tgcctcgctc accatcgcag ccgtcgcctt ggtgtggtgc ttggaaaaat
    36721 acagtatgga gttggtcaaa tcacacaagg ccgtctgaaa acgcagcaca cttcaattca
    36781 aatacagtta aagctcaaac catgcaaccc atccgatacc gaaccgacct caccccctac
    36841 aacactttcg gcctgcacgc ccaagcccga gcctttatcg cgcttaaaca cgccgacgaa
    36901 ttgcgcgaca tcgtccgact gcccgagttc gaccgcgata ctgttttatg gctgggcggc
    36961 ggcagcaaca tccttttgat gcaggattac gacggcttgg tcgtccatat ggaaaacaag
    37021 ggcatacgcg agattgcgcg ctcagacggc atggttctga ttgaagcgca ggcgggcgaa
    37081 atttggcacg atttcgtgtt gcacaccgtc gcgctgggtt tgagcggttt ggaaaacctg
    37141 agcctgattc cgggtacggt cggcgcgtcg cccgtgcaga acatcggcgc atacggcgtg
    37201 gaggcgaaag atgtgattca cagcgtgcgc tgcttcgatt tggatacgga aacctttgta
    37261 accctttcca atgccgactg ccgcttcgcc taccgcgaaa gcctgttcaa gcaggaaggc
    37321 aaagggcgtt atgtgattgt ttcggtcgta tttgcattaa aaacgcattt tgtgccgaac
    37381 ttgggctacg gcgatttggc ggcgaaggtt gccgaattga gtgcaggtag ggaggcgacg
    37441 gcgaaagacg tttccgatgc ggtatccgcc atccgcaaca gcaaattacc cgacccgaaa
    37501 gttttgggca atgtcggcag tttctttaaa aaccccgtcg tcagcgcgga aaaagccgcc
    37561 accctgttgc agcggcatcc cgatatgccg cgctatccgc agcccgacgg ttcggtcaaa
    37621 ctcgccgccg gctggctgat cgaccaatgc cgtctgaagg gcttccaaat cggcggtgcg
    37681 gcggtacatg accggcaggc tctggttttg gcgaacaaaa acaatgcctc ttcagacgac
    37741 atctggaaac tggcgcaaca cgtctgcaat acagtattta ctcgatttca ggtagaatta
    37801 cacgccgaac ccaattggct gcccgcttcg ttcagcctgt aaatccaagg agtatgcccg
    37861 tgacccgcat tgcaaaaacc aatacttaca cccgcatcat cgacgccagc cttgcgcttt
    37921 tcaacgagga aggcgagcgc aacatcagca ccaaccatat tgccgcccac ttgggcatca
    37981 gtccgggcaa tctctattac cacttccgca acaaagacga aatcatcgtc caactgttca
    38041 aacgttacag cgaagccctg ctggaatacc tcaacgaagc cgtgttgccc tctgatgtgg
    38101 aagactctat caactatatg gcaggcattt acgatgtcat gtgggaatac cgcttcctct
    38161 ttagcgacgt aaacacccta cttgcgcgca gtgccgaatt gttgggcgaa cacaatacct
    38221 ttacccaagc caaagtctcg ccgcttctag tcaaactgct gacccagctc aacgggctga
    38281 acgtcatcaa cgccgaccaa accgctatga acgacctcgc cgtcaatatg tggatggtta
    38341 cgaaatactg gttcgacttc gacagttccc tgcgcggccg taccaagctg accgaagact
    38401 ccaaagcacg cggcgtccgt cgtaccttaa gcctccttcg cccctatctt ttgcccgaac
    38461 accgcgcgga atacgaccgc aaaatcagca acggcaaccc gtaaacccat aacgtccgaa
    38521 tcagccttca gacggcactt acccgacggt atcagcgaat gcgcgcagtc cccccgcccc
    38581 atatctccat cacaggccta ggttacctcg gcctgccgct ggcacaaaag ttttaccgac
    38641 acggcagccg cgtcgccgcc ataaaacgca gcctgacttc ggacgacatc aacctgccca
    38701 tacacctcga caccttcgac ctcaaccgta ccgatacctt tcagacggca ttgtggcggc
    38761 atcacgccga caaacccgtc tggttcttcc tgctgccgcc ctcctttttg gcgcattacg
    38821 ccgacaccgt caaacaatgg gcagagcttg cccgggcgtg caacgtgcaa cacctgattt
    38881 tcacaagcag cacaagcgtt tacggcgata aagcgcgaga atgcaacgaa accgccgcac
    38941 ccgatccgca aaccgagtcc gcccgacaaa tccttgccgc cgaacaacac ctgctcgaca
    39001 acggcgttcc gaacatcgac atcctgcggc tgggcgggct ttattgcgcc gaacgccatc
    39061 ccgtcagccg cctcgtgcaa aagcaaaaca tccaaggcgg caaccggcct gtcaatatcg
    39121 tccaccgcga catcgccgtc gaaaacctgt ttcagacggc atccaacccc ggcggcaggc
    39181 ggctgaaaaa cattatcgaa ccgcgccacc cgacacgccg cgaattttat acggaagaag
    39241 ccgccaaact cggcctgccc gcgccggatt tctcacccga cgacagcgtg ggcaaaatca
    39301 tccgtaccgt ttgcgataac ggcttaagcc tgtaaaataa gcggcaacag caacaacagg
    39361 cattctccgc cccaatcaga aaatatgtcc gccctcctcc ccatcatcaa ccgcctgatt
    39421 ctgcaaagcc cggacagccg ctcggaactt acctcctttg caggcaaaac actgaccctg
    39481 aacattgccg ggctgaaact ggcgggacgc atcacagaag acggtttgct ctcggcggga
    39541 aacggctttg cagacaccga aattaccttc cgcaacagcg cgatacggaa aatcctccaa
    39601 ggcggcgaac ccggggctgg cgacatcagg ctcgaaggcg acctcatcct cggcatcgcg
    39661 gtactgtccc tgctcggcag cctgcgttcc cgcgcatcgg acgaattggc acggattttc
    39721 ggcacgcagg caggcatcgg cagccgtgcc accgacatcg gacacggcat caaacaaatc
    39781 ggcaggaaca tcgccgaaca aatcggcgga ttttcccgcg aacccgagtc cgcaaacacc
    39841 ggcaacgaag cccttgccga ctgcctcgac gaaataagca gactgcgcga cggcgtggaa
    39901 cgcctcaacg aacgcctcga caggctcgaa cgcgacattt ggatagacta accttcagac
    39961 ggcatccgac atgaacagcc cttttcacaa catcggtatc gtaacccgcc ccaacacgcc
    40021 cgacatccaa gataccgcac acacgctgat tacctttttg aagcagcacg gctttaccgt
    40081 ctatctcgac gaagtcggcg taagggaatg ctgcatctat acccaagaca ccgacggctg
    40141 ccatatcgtc aacaagaccg aactggggca atactgcgac ctggtcgccg tcttaggcgg
    40201 agacggcacc tttctctctg ccgcccgcga aattaccccc cgcgccgttc cgattatcgg
    40261 catcaaccaa ggtcatttgg gctttcttac ccaaattccc cgcgaatata tgacggacaa
    40321 gctattgccc gttttagaag gaaaatacct tgccgaagag cgcatcctga ttgaagccgc
    40381 actgatccgc gaaggcaaaa ccgccgaacg cgccctcgcc ctcaacgatg ccgtcctctc
    40441 ccgcggcggt gccggacaga tgattgaatt tgaagtcttc gtcaatcagg aattcgtcta
    40501 tacacaacgt tcggacgggc tgattgtctc cacccccacc ggatcgaccg cctattcgct
    40561 tgccgccggc ggcccaatca tgcaggcagg attacacgcc ttcacgctcg tccccatctg
    40621 cccgcaatct atgactaacc gccccatcgc cattccagac acgtccgaaa tcgaaatcct
    40681 cgttacccaa ggcggcgacg cgcgcgttca tttcgacgga caatccttca tcgacgtgca
    40741 aaacctcgac cgcatcatca tccgccgcta ccacaatccc ttacgcatcc tacacccgac
    40801 cgactaccaa tatttcaaaa ccctgcgcca aaaactgcac tggggcgagc aattagtcta
    40861 agccggcctt cacccgaaag tacccatgaa ttccaaaatt tcctccgacc atacagaaga
    40921 taccatacgc ctctccaaac gtatggcaca actggggctt tgttcgcgcc gcgaagccga
    40981 cggccatatc gaacagggct gggtaacggt caacggcaaa accgccgtac tcggtcagaa
    41041 agtttcaccg gcagaccgta tcgaactgaa caagaaagcc cacgaacagc aggcggcacg
    41101 cattaccatc ctgctgaaca aacccgtcgg ctatgtcagc gcacaagcgg aaaaaggcta
    41161 taaatccgcc gccgaactga ttacccctga aaattgctgg gaaggcgaca ccagccgcat
    41221 cagtttcgat ccgaaacaca aaatcggtct tgcccccgcc ggcaggctcg atatcgattc
    41281 agtcggattg ctggtgttga cgcaagacgg ccgtatcgcc aagcagctta tcggcgaaaa
    41341 cagcggcagt gaaaaagaat atttggtgcg cgtgcgcggc aaattggacg aaaaaggact
    41401 tgccttactg aatcacggat tgagtttgga cggtgataaa ctgcgtcccg ccaaagtaga
    41461 atggcaaaat gaagaccaac tgcgcttcgt gttgaaacag ggtaaaaagc ggcaaatccg
    41521 ccgtatgtgc gaactggtcg gactgcgcgt cgtcgggctg aaacgcatcc gcatgggcaa
    41581 ggtcaaactc ggcaggctgc cgcccggcaa atggcgttat ctcgctcccg gcgaatcgtt
    41641 ttaaataagc attaaaaaat gccgtctgaa aatccttgcg ttttcagacg gcactgcctt
    41701 atgcccaaat aacggtttct tccaagggcc tgcgcgcttt cgtgacgatt tcctgaaccc
    41761 ggtagccgaa tgtcgcggca acggacacgc cccattctgc cgcatcgaac aaaccgaact
    41821 gcccggacaa tacgcgttcc atatcggcat agttgaaacc ttccaccggg caggaatcga
    41881 tacccgccat cgccgcaccc gtcatcatgt tggctaacgc gatataggtc tggcggcagc
    41941 accagtcaaa caaggcgcgc gaatcgtcca aaatcttgat gtcgtcagct tgaaacgcct
    42001 gatacctcgc caaagatttt tctacggcat ccggttcggt aacgccgcgc cgtttgagac
    42061 tttccaacat aaacgggctg tcgaagcggg catttttctt cgccaaaaac accaccaaat
    42121 gactggcggt atccaaagca tccgccatac cccaagaaaa cagcttgatt gcctgtcgga
    42181 tttcggggtt ttgaaccaca acaaaccgcc aaggctccga accgaccgaa ctgggcgaca
    42241 aacgcccgag ttctaaaata aactgaaaat cctcggcact gattttacgt gcgcatcgta
    42301 atgccggcac gatttgcggt ttttaaatgc ggatagaacc tgctctttgc ttaatactgt
    42361 catcgtgcat ccttttatta ctttgattgc aaattgattt caaaccgata cggcactgcc
    42421 gcaccctata agccgtcccc gccatcaagc ttcacccgga ttttacccat ctctctacgg
    42481 ctgggaaaac aaagcggcgg cagattcggg aataactccc caatgccttt cagacggcat
    42541 ttggacatcg caaacgcgcc tacgggtagg cgatataggc ataagccgaa tggttgtgga
    42601 tagactcgaa attctcgctc tcgacgacga aactcttgat gcgtttgtcg gcaatcagcg
    42661 aagtagcgac atcgcgcacc atatcttcca cgaatttcgg gttttcgtag gccttttcgg
    42721 taacgtattt ttcatcgggg cgtttgagca ggccgtagag ttggcagctc gcctgcgttt
    42781 ccacataatc gataacttcc tcgataccga cttcggcatc ggaagtcagg ctgacggtaa
    42841 cgtgcgaacg ctgattatgc gcgccatatt gggaaatttc tttggaacac gggcaaagcg
    42901 aggttacggg aatcatgacc ttcatactgt ggccgtatgc cccgtctttc atttcacccg
    42961 tgaggctgac atcataatcc agtaaggacc ggataccgga taccggcgcg gttttcttgc
    43021 ggaaaaacgg aaaagaaacg ctgattttgc cggcgcggga atccaaaagc gcgaccattt
    43081 cggcagtcag cctatgcaat tgtgcgaaat ccaagacttc ggtatgttgc tccatcaatg
    43141 cgacaaagcg cgacatatgc gtccctttct gctcggcggg cagataaacc gtcatcgtca
    43201 ggcgagcaac cgtggattgt gttccttcgg cggttttcag ggtaatcggg aagcgcaggt
    43261 ctttaatgcc gacctgattg atcggcaggt tgcgcaaatc tctgctggat tgcacgtctg
    43321 caatggcgtt catgcgttgt ttgtccttaa tattcagata attattgaga tgtgtgtatt
    43381 gtatggcagg cagatgccgt ctgaaaaaac gctgtcggcc gtcctgcctg caaatgcggg
    43441 attatatcac ttgcttttgg cggctgcatt gattgtttct attttcggga tagtgataaa
    43501 atcccgactt atccatatct tacaaggcag aaacagatga aattcccgac ccgtgccatc
    43561 cattccggct acgattgcga cgaacacaac cgcgcgctga tgccgccgat ttatcaaaac
    43621 agtatgtttg cgctgcacga aatcggcgaa aatgtgcctt accgttattc gcgcctgagc
    43681 aacccgaccc gtcagatttt ggaagacacc gttgccgatt tggaacacgg tgcggcaggt
    43741 tttgcgtttt ccagcggtat ggcggggatt gatgccgtgt ggcgtacctt cctgcgcccg
    43801 ggcgatacca tcgtcgcggt cgccgatatt tacggcggcg catatgactt attggtcgat
    43861 gtttatcaaa aatggggggt gaacgttgtt tttgccgatt tgggcaatcc cgataatttg
    43921 gacgaattgc tcaaagcgca caatgtcaaa ctggtttggc tggaaatgcc gtccaatccg
    43981 cttttacgct tggtagacat caaagccctt gcagcgaaag ccaaagcagc cgacgcgctg
    44041 gtcggcatcg acaacacctt tgccacgccg tatctgcaac agcccttgga tatgggttgc
    44101 gattttgcat tccattccgc taccaaatat ttgtgcggcc attccgacgt attgatgggc
    44161 atcgtcgttg ccaaaaccaa agagctggct cagcctttgc acgatatgat ggtgcatacc
    44221 ggcgcgattg caggtccgac ggactgctgg ctggtgttgc gcggcatcaa aacgcttgcc
    44281 ctgcgtatgg aggcacactg caaaaacgca ctcgaaattg cgcgccgttt ggaagcccat
    44341 cctgccattg aaaaagtgtt ccatcccggc ctgccgtctc acgaacatta cgcactcgcc
    44401 caagcgcaaa tgcccaaagg catcggcggc gtggttacgg tttatctcaa aaacgacacg
    44461 cgcgaagcgg caaacagcgt gattaaaaac atgaaactgg tcaaaatgac ctccagcctc
    44521 ggcggcgtgg aaagtctggt caaccattgc tattcccaat ctcacagcgg cgtgccgcat
    44581 aatgtgaaaa tggaaatggg catcagggtc ggactcctgc gcttctccat cggtattgaa
    44641 gacgtagatg atatttggaa cgatatttcc gccgcactcg atacaacttt gtaaaccgta
    44701 aaaaatgccg tctgaaacca tagtttcaga cggcatttca atattgcgaa tcaaatatca
    44761 acgcttcagc atctttgccg cctcaatggc gtaataggtc aaaatcccgt ccgcacccgc
    44821 acgtttgaat gccagcaggc tttccaaaac cactttgccg ccgtccagcc agccgttggc
    44881 aaccgccgcc tgcaacatcg catattcgcc cgaaacctga taggcataag tcggtacgcc
    44941 gaactcgtcc ttcacgcggc ggacaacgtc caaatacggc aaaccgggct tcaccatcac
    45001 catatccgca ccttcctgaa tatcgagcgc cacttcatgc agcgcctcat cggtatttgc
    45061 aggatccatc tgataggtct ttttatctgc ctttcccaaa ttgcccgaac tgcctaccgc
    45121 atcacggaaa gggccgtaga atgcagaagc atatttggcg gaatatgcca taatccgcgt
    45181 atggatatgt ccggcatcct ccaaagcctc gcggatggcg ccgatacgcc cgtccatcat
    45241 atcggaagga gcaacgacct gcgtgcccgc ctctgcatga cataaagcct gtttcaccaa
    45301 gacttctacg gtttcatcat tcatcacgta accgttttcg tccgtcagtc cgtcctgacc
    45361 gtgcaccgta taaggatcga gcgcgacatc cgtcataatg cccagttcgg gaaacctctc
    45421 gcgcaaggct cggacagttg acggcacgag tccttcggga ttgtacgcct cctgcgcacg
    45481 cccggttttg tttgccgtaa ccacgggaaa gagtgccaac atcggaatac cgagcttcac
    45541 cgcctcttcc gccgtaaaca gcagcctgtc caaactctga cgcttcacgc ccggcataga
    45601 aggcacatcc tcctcgcgcg ccgccccctc caatacgaac accggataaa tcaaatcatc
    45661 ggcggtcagc atatgctcgc gcatcaggcg gcgtgaaaaa tcatccctgc gcatacggcg
    45721 catacgcgaa gccggaacat tgcggtaagg aaattgcata agccctccaa tcatcctgtc
    45781 aacaattcaa atcatacgga agccgccgca acttgcagac ggcatcgagc ggtaatcaaa
    45841 atccgttttc accggaaagg gtttcggcac gccgaaatgc cgcgccgaag gacagcatgc
    45901 cgcccattga aagaacaata gcaaaacaac cacttcaggc tgtcaggcct cgatattctt
    45961 acgccacaat accaaaagtt tcccgatatg ccgaacaggt tgcgcgtcaa ccgcctcaca
    46021 caaggtattg cagatttcga tacgctcggc acggtcgtcg ccgaatacgc gcactttaat
    46081 cagctcatgc gccgtcagtg ccgcatcggt ttccttgatg actgcatccg tcagaccctg
    46141 ctgaccgacc atcacaacag gatggagatg gtgcgcgcgc gctttcagtt ccaaaatttc
    46201 tttggtgttc aatttggtat ccgccatttt cctactgttt gaataaaaag gataatgcag
    46261 cattgtacgc gatttggcac ggcaacggca aaattaacgt acaatacacc gtttacattc
    46321 cgacccgcga accactatgg ctgtacgttc caaatcctca aaagcgtggc tgcacgaaca
    46381 catcaacgac cactacgtcc atatggcgca aaaagacggc taccgcgccc gtgccgcata
    46441 caaacttttg gaaatcaacg aaaaagacaa gataatcaaa cccggcacgg tacttgccga
    46501 cttgggcagc gcgccgggaa gctggtcgca ggttgccgcc aagctgacgg gtacttccgg
    46561 aacggttttc gccttggaca tcctgcctat ggaagccata gggggcgtgt ccttcattca
    46621 gggcgacttc cgcgagaacg acgttttggc gcaattcgaa accttgttgg acaaccgccc
    46681 gctcgacctt gtaatctgcg atatggcgcc caatatgtcg ggaaacgccg taagcgatca
    46741 ggcacgcagc ttttatctgt gcgaactggc tttggacttc gcctcgcaac acctgaaaac
    46801 cggcggcagc tttttggtca aagtctttca gggtgcaggc tatcaggaat acatggcagc
    46861 catgcgcgaa attttcggca ctgtgcagac gcgcaaaccc gaagcctcgc gcaatcgctc
    46921 cagtgagatt tatttattgg gcaaaaataa acgctgacaa tacagacggc gtgctttaca
    46981 atcatttccg ttttacacct caattatgga gccttgctaa gtggggaaca cctttaaatc
    47041 aatccttgtc tgggtcgcct tgggtatcgg cctgatggct gcgttcaacg ctttggacgg
    47101 caaaaaagaa gacaacgggc aaatcgaata ttctcagttc atccgacagg tcaacaacgg
    47161 cgaagtatcc ggcgtcaaca tcgaaggatc cgtcgtcagc ggctacctga ttaagggcga
    47221 gcgcaccgac aaaagcacct tctttaccaa cgcgcctttg gacgacaacc tgattaaaac
    47281 actgctcgac aaaaacgtcc gcgtaaaagt aacgccggaa gaaaaaccga gcgcgctggc
    47341 tgccctgttt tacagcctgc tgcccgtcct gctgctgatt ggcgcgtggt tctactttat
    47401 gcgtatgcag acgggcggcg gcggaaaagg cggcgcattc tcattcggca aaagccgcgc
    47461 acgcctgctg gacaaagatg ccaacaaagt gacctttgcc gatgtcgccg gctgcgacga
    47521 agccaaagaa gaagtgcagg aaatcgtcga ttacctcaaa gcaccgaacc gctatcaaag
    47581 cttgggcggg cgcgtgccgc gcggcatcct gctggcgggc agcccgggta cgggtaagac
    47641 gcttttggca aaagccattg caggcgaagc cggcgtgccg ttcttcagca tttccggttc
    47701 cgactttgtc gaaatgttcg tcggtgttgg tgcgagccgc gtccgcgata tgttcgagca
    47761 ggcgaagaaa aacgccccct gcatcatctt tattgatgaa atcgacgcag tcggccgcca
    47821 acgcggcgca ggtttgggcg gcggcaatga tgagcgcgag caaacattaa accaattatt
    47881 ggttgaaatg gacggttttg agagcaatca gactgtaatt gtgattgcgg caaccaaccg
    47941 ccccgacgta ctcgatcctg cgctgcaacg ccccggccgc ttcgaccgcc aagtcgtcgt
    48001 ccccctgccg gacatccggg ggcgcgaaca gattttgaac gtccattcta aaaaagtgcc
    48061 tttggacgaa tctgtggatt tattgtccct cgcgcgcggc acgcccggtt tttccggcgc
    48121 ggatttggcg aacctggtca acgaagccgc cctgtttgcc ggccgccgca acaaagtcaa
    48181 agtcgatcaa agcgattttg aagacgccaa agacaaaatc tatatgggtc cggaacgccg
    48241 cagtatggtg atgcacgaag acgaaaaacg tgcgacggcg tatcatgagt ccggacacgc
    48301 gattgttgcc gaaagcctgc cctttaccga ccccgtccac aaagtaacca ttatgccgcg
    48361 cggacgtgcg ctgggtctga cttggcagct tccggagcgc gaccgcatca gtatgtataa
    48421 agatcagatg ttgagccagc tctccatcct gttcggcgga cggattgccg aagacatctt
    48481 cgtcggacgc atctccaccg gtgcatcaaa cgactttgaa cgcgcaaccc aaatggcacg
    48541 cgaaatggta acgcgctacg gcatgagcga caaaatgggc gtgatggttt atgcggaaaa
    48601 cgaaggcgaa gtcttcttgg ggcgcagcgt aacccgttcg caaaacattt ccgagaaaac
    48661 ccagcaggac atcgacgcgg aaatccgccg gattttggac gagcagtatc aggttgccta
    48721 caaaatcctc gatgaaaacc gcgacaagat ggaaacgatg tgcaaagccc tgatggaatg
    48781 ggaaaccatc gaccgcgatc aggtactgga aattatggcg ggcaaacaac ccagcccgcc
    48841 caaggattac agccacaacc tgcgcgagaa tgcggacacg gcggaagata acgcgccgca
    48901 cgctccgact cgggaaaaaa ccgaagcacc tgccccggca gacaccgcct cgacagagtc
    48961 cgggcagcag cctgaaaaca aggcttaact tcccgaacaa acggcagccc gcaagctgcc
    49021 gttttcccat cccgttttca gacggcattt tccgctcaat gccgtctgaa acgccaaagc
    49081 cgtatcggtt tttgcgaatc tgcgatatgg cggtttaaat tgaaatcaga acggggcaag
    49141 gctgtaccgg tttaaaatta aaccgctata ataccgccct tcgaatacac gcccgcattg
    49201 gtgatgcccg tgaaaaaagt tgaaaaaaac atcttggtgc tgcacggcgc ggacaaaatg
    49261 ttcgagctgg tcgacaaggt tgaggattac ccgcactttc tgccgtggta cagcaagacc
    49321 gaggtcatcg ggcgtagcgg caacgaactg aaggcgcggc tgtttatgga ttatatgcgc
    49381 gttcgccaat cgtttgccac gcacaaccgc aacatccccg gcagggaaat ccgtatggaa
    49441 ctgctcgaag gcccgttcaa aaccttacgt ggaacgtgga aatttatcga tttgggcgac
    49501 gatatgtgca aaatcgaatt caatttggaa tacgattttt ccaatgccgt tttgtccgcc
    49561 ttaatttccc ccgtcttcaa ccacctttcc gccacgctgg tcgaagcgtt cgtcaaagag
    49621 gcagaccgcc gttatgcttg aaattgagat tgtgtacggt ctgcccgacc gacaggtttt
    49681 gaaaaccatg cagcttgccg agggaacaac cgtccgcacc gccgcactgc aaagcggttt
    49741 ggacggcata tttgaaaatt taaacctgca ttccgcgcct ttgggcattt tcggcaaagc
    49801 cgtcaaagac gacacgccgc tgcgcgacgg cgaccgcatc gaagtgtacc gcccgctgtt
    49861 gatcgacccc aaagaagcgc gccgcaaacg cgttcaaaat caagaagaat aaccatgccg
    49921 tctgaagcct tcagacggca ttcgacagaa acacggaaaa tatcatgtca aacacaatca
    49981 aaatggttgt cggcttgggc aacccgggca aagaatacga acagacccgc cacaatgcgg
    50041 gcttttggtt cctcgacgaa ctggcgtgga aatggaaggc ttcgtttaaa gaagaaaaaa
    50101 aattcttcgg cgaagttgcc cgcgccgccc tgcccgacgg cgatgtttgg ctgctcaaac
    50161 cggccacgtt catgaaccgt tccggacagg cggttgccgc gcttgcacag ttctacaaaa
    50221 tcaaacccga agaaatcctc gtcgtccacg acgaactcga catcccttgc ggacggatca
    50281 aattcaaact cggcggcggc aacggcggac acaacggctt gaaagacatt caggcaaaac
    50341 tcggcacggc agactattac cgcctgcgcc tcggcatcgg ccaccccggc gaccgcaacc
    50401 tcgtcgtcgg ctacgtcttg aacaaaccga gcgcggaaca ccgccggcaa atcgacgatg
    50461 ccgtcgccaa atccctgcag gccgtacccg acatcatttc cggcaaatgg gaagaggcaa
    50521 cgcgcttcct gcacagcaaa taatccaatg ccgtcttaag ccctttcaga cggcattttc
    50581 ccgatttccg tatccgaaca gtcatgaccg aactcaagca gcttatccaa accgaatcca
    50641 tccccgtcat cgaagaaacc ctcgatttcc tgctgtacga atgcagcatc gacgacgcac
    50701 cgtccgccga agaagtggca caatggcgcg acatacttgc cgcacgcggc ggcaaattcc
    50761 tgcgcctgtc caaaatctgc caaacgtggc tggacgagga ggcggcatga agctgccgcg
    50821 caaccgcttc agcctgcttt ccgcattgtg gtttgccggc ggcatctatt cgctgctctt
    50881 caaagctgcc gacaccgcgc cgccgccgtt tccacatttc gacaaagcag cacaccttgc
    50941 cctgtttttc gcacaaatct tgcttctggc caaagcattc aaaaccggaa aacttcccat
    51001 cccctaccgc agcctgattg cgttcgcctt ctgttttgcc gtcggcagcg aatgcgcgca
    51061 ggcatggttt actgcaacgc gaaccggcag tttgggcgat gtccttgccg acctgacggg
    51121 cgcagccctt gccctctttg ccgcgcgttc tgcctgccgc ccggactaaa tcggtttatt
    51181 tctcccaaac aggatgaatc cccctgatgc atcctgtttt tccccttaca tatcaatccc
    51241 atctcataaa caaaaccgac aaatcgttta caatatattt acactacacc gaattacaaa
    51301 tatactcgaa gcggttgcag aaccgacggt ttcgcaaacc gtttcggatt gcacgacaca
    51361 aaagaaaaac cgattttgtt gccttaaagg agcattcatg aacctgcatg caaaggacaa
    51421 aacccagcat cccgaaaacg tcgagctgct cagtgcgcag aagccgatta ccgactttaa
    51481 gggtttgctg accaccatta tttccgccgt cgtctgtttc ggcatttacc acatcctgcc
    51541 ttacagcccc gatgccaata aaggtatcgc gctgctgatt ttcgttgccg cactttggtt
    51601 taccgaagcc gtccacatta ccataaccgc actgatggtg ccgattctcg ccgtcgtact
    51661 cggtttcccc gacatggaca tcaaaaaggc gatggctgat ttttccaacc cgattatcta
    51721 catttttttc ggcggcttcg cgcttgccac cgccctgcat atgcagcggc tggaccgtaa
    51781 aatcgccgtc agcctgttgc gcctgtcgcg cggcaatatg aaagtggcgg ttttgatgtt
    51841 gttcctcgtt actgcctttc tgtccatgtg gatcagcaac accgccaccg ccgcgatgat
    51901 gctgcctcta gcaatgggta tgctgagcca cctcgaccgg gaaaaagaac acaaaaccta
    51961 cgtcttcctc ctgctcggca tcgcctattg cgccagcatc ggcggcttgg gcacgctcgt
    52021 cggctcgccg cccaacctga ttgccgccaa agccctaaat ctggacttcg tcggctggat
    52081 gaagctcggc ctgccgatga tgctgttgat tctgcccttg atgctgctct ccctgtacgt
    52141 catcctcaaa cctaatttga acgaacgcgt ggaaatcaaa gccgaatcca tcccttggac
    52201 gctgcaccgc gtcatcgcgc tgttgatttt ccttgccaca gccgccgcgt ggatattcgg
    52261 ctccaaaatc aaaaccgcct tcggcatttc caatcccgac accgttatcg ccctgagtgc
    52321 cgccgtcgcc gtcgtcgtct tcggcgtggc gcaatggaag gaagtcgccc gcaataccga
    52381 ctggggcgtg ttgatgctct tcggcggcgg catcagcctg agcacgctgt tgaaaacatc
    52441 cggcgcgtcc gaagccttgg gacagcaggt tgccgccacc ttttccggcg cgcccgcatt
    52501 tttggtgata ctcatcgtcg ccgccttcat tatttttctg accgagttca ccagcaacac
    52561 cgcctccgcc gccctgcttg taccgatttt ctccggcatc gctatgcaga tggggctgcc
    52621 cgaacaagtc ttggtattcg tcatcggcat cgccgcatct tgcgccttca tgctgccggt
    52681 tgccacgccg cctaacgcga ttgtgttcgg cacgggctta atcaaacaac gagaaatgat
    52741 gaatgtcggc atactgctga acatcctatg cgtagtattg gttgctctgt gggcttatgc
    52801 tgtactgatg taaaccatcg acctaaacaa caagaccgtc tgaaagaata tttttcagac
    52861 gaccttgaag tttgtcgttc agacacaatt tgtcgaataa ttcaaaaccg gattctaacg
    52921 aaaggaaaac ccatgattat cctgcacacc aacaaaggcg acatcaaaat cgaactcgat
    52981 ttcgacaaag cccctgttac cgccaaaaac ttcgagcaat acgtcaaaga cggcttctac
    53041 gacggcgtaa tcttccaccg cgtcatcaaa ggcttcatga ttcaaggcgg cggcatggat
    53101 gaaaacatga acgaaaaaga aacccgcgat ccgattcaaa acgaagcgtc caacggcctg
    53161 cccaacgata aatacaccat cgccatggcg cgcacttccg acccccattc cgccggcgcg
    53221 caattcttca tcaacactgc cgacaacgct ttcctaaact tccgttctaa agagctgtac
    53281 ggcaaaaccg tcgtccaaga ctggggctat gccgtattcg gtaaagtcgt tgacggtttt
    53341 gacgttgtcg atgccatcga aagcgtatcc accaaacgcc acggctacca cgacgatgtg
    53401 ccgaccgaac ctgtcatcat cattaaagcc gaagcagtat aaaccggcaa tccgaaagca
    53461 gcctgcataa aaggctgctt ttatttcaga cggcatgtta tttgatgtaa ccgggctatg
    53521 ccgtctgaaa accggacgca agccttcaga cggcataccg ctatgttaaa atacgcccgt
    53581 ttttcctaaa cggacacaaa ggaaacctta tggcaagcat cacccgcgac atcttcaaag
    53641 cctacgacat ccgtggcatc gtcggcaaaa ccctgaccga cgatgccgct tatttcatcg
    53701 gcagggccat cgccgccaaa gccgccgaaa aaggtatcgc ccgcatcgcg atcggacgcg
    53761 acggacgctt gagcggcccc gaactgatgg agcacatcca acgcggcctg accgacagcg
    53821 gtatcggcgt actcaatgtc ggcatggtta ccactcctat gctctacttc gcagccgtca
    53881 acgaatgcgg cggcagcgga gtgatgatta ccggcagcca caatccgccc gattacaacg
    53941 gtttcaaaat gatgctcggc ggcgacacgc tcgcaggcga agccattcaa gaacttttag
    54001 ctattgttga gaaagacggt tttgttgccg ccgacaaaca aggcagcgta accgaaaaag
    54061 acatctccgg cgcataccac gaccacatcg tcggacacgt caaactcaaa cgcccgataa
    54121 acatcgccat cgacgcgggc aacggcgtgg gcggcgcgtt tgccggcaaa ctctacaaag
    54181 gtttgggcaa cgaagtgacc gaacttttct gcgaagtgga cggcaatttc cctaatcacc
    54241 accctgatcc ttccaaaccg gaaaacctgc aagatttgat tgccgcgctg aaaaacggcg
    54301 atgccgaaat cggcttggcg tttgacggcg atgccgaccg cttgggcgtg gttaccaaag
    54361 acggcaacat tatttatccc gaccgccaac tgatgctgtt cgcccaagac gttttgaacc
    54421 gcaatcccgg cgcgaaagtc attttcgatg tcaaatccac acgcctgctt gccccgtgga
    54481 ttaaagaaca cggcggagaa gccataatgg aaaaaaccgg ccacagcttc atcaaatccg
    54541 ctatgaaaaa aaccggtgca ctggttgccg gcgaaatgag cggacacgtt ttctttaaag
    54601 aacgctggtt cggcttcgac gacggcctgt atgccggcgc acgcctcttg gaaatcctgt
    54661 ccgcctccga caatccgtcc gaagtgttgg acaacctgcc gcaaagcatt tccacgcccg
    54721 aactcaacat ctccctgccc gaaggcagca acgggcatca agttatcgaa gaactcgccg
    54781 ccaaagccca atttgaaggc gcaaccgaaa tcatcaccat cgacggcctg cgcgttgaat
    54841 ttcccgacgg cttcggtctg atgcgtgctt ccaataccac gccgattttg gtgttgcgtt
    54901 ttgaagcgga tacgcaagca gccatcgagc gcattcaaaa ccgattcaaa gccgtcatcg
    54961 aaagcaatcc gcatttaatc tggcctctgt aaaaatagaa aaaatgccgt ctgaaacttt
    55021 cagacggcat ttttcacaag tagccaatca aatcttggca gattggattt ggcttaaaaa
    55081 tctccgcgtc cgttcgtgtt tgaggtggtc gaacaactct ttcgggctgc cctgctctac
    55141 gataacgccg ccgtccatca cgacgacgtt cgtggcaact tccagcgtga acttgatttc
    55201 gtgggtaacg acgaccatcg tccaaccttc ccgcgccaat tccttcatgg cgtccaacac
    55261 gtcttgcacc aactcggggt ccagcgcgga agtgggttcg tcaaacagca tcaattcagg
    55321 ctgaatcgcc agtgcgcggg cgataccgac acgctgctgc tgaccgccgg aaagctggta
    55381 gggatacaaa tccactttat cgcccaagcc gactttttcc agcagtttca aagcctcttc
    55441 gcgcgcttgg gcggcaggct tgccctgtac ggcaaccggc ccttccatca cgttttccaa
    55501 cacggtttta tgcgggaaga ggttgtattg ttagaatacc attccggact tgcggcgcag
    55561 tgccaaaata tcgtgtttgc ttgttttttt ggaaaaatca atgcgtaacg gccgcgcgtt
    55621 gtcgaactcg atttgtccgt cttcgggcat ttccaacgcg tttaggcagc gcagaaatgt
    55681 tgttttaccc gagccggaag gcccgaggat gacgaccacc tgccctttgc ccacatccaa
    55741 atcgatgccg cgcaaaatgg tgttttcgcc aaaggtctta tggatattgc ggattttaat
    55801 catgacaact ccttatttgg cgacataacg gtcgaaacgt ttttccaaac gcgcctgaat
    55861 caaaaacagc actttacaga aacaccaata aaccaatgca gcttcgatat agacaggcaa
    55921 aaagtcataa gtgcggtttg ccgtttcctg tgcgacacgg aaaagctccg ttaccgttac
    55981 cacggcggca agcgaggtgt ttttgaacaa gccgataaac tcgttgctca acggcggaac
    56041 ggcgacgcgg aatgcctgcg gtgcgacgat gcggcggaac gtctgcatat aggtcatacc
    56101 gatggagaaa cctgcttccc actgcccttt cggcacggac aaaatcgccg cgcgtatggt
    56161 ttcggaagcg tatgcgccga cattgagcga aaagccgatg atggcggcgg gaatcggatt
    56221 gatatagatg ccgacggacg gcagcccgta aaacacaatc acaagctgaa ccaacagcgg
    56281 cgtaccgcga acgacggaaa tataaaattc caccagcttc aacaagcatt tttggaaaat
    56341 accgccggaa ggcatgattc ttaccaaagc aacggctacg gcaataatca tgccgataac
    56401 gaaagaagcg atcgccaaag gcaaagacac tgtaaagccg gctttaacca tgggccaaaa
    56461 cgcgctgatg agcatatcag cgcgtgtttc cgtcataaac ggcagagagg caaggaaatt
    56521 attgaacact gatgtctttt ccgaagaatt gttcgcccag ttttttcaac gtgccgtcgg
    56581 ctttcagctc gttgattgcc gtgctgaatt ttgccacggc ctcgtcattg cccttgttga
    56641 caatcagacc ggaaccgact ttttcatcgg caggcgcgga ccacacgatt ttcacccccg
    56701 cattcgggtt tttcttcaga tagtccaaaa ccgccaattc atcgttcaac gtcgcatcgg
    56761 cgcgtttttg ttcaatcagg gtcagcgatt gcgccaaacc gtccaccggc acgagttgcg
    56821 cacctgcggc tttggctttt tcgccgtagt tgctggtcag ggattgcgcg gttttcacgc
    56881 ctttgatgtc ggctatggat ttaatgttgc tgtcgttacg cgcaaccaaa accgcaccgc
    56941 tccagctgta aggttcggat ttgtcaaatg tcgcctggcg ttcggggctg gtcaggccga
    57001 cttggtttgc caccacgtcg aaacgtccgg ctttcaaacc cgccatcatc gaatcccatt
    57061 gcgtttcttt aaactcgact tttacgccca gtttttccgc cacggcgcgg gttacttcca
    57121 cgtcgtaacc ggtcagtttg ccgtctttgt cgtggtaggt aaacggtgcg taagtgcctt
    57181 ccgtgccgac ggtaaccgtg cctttattgt tgatgcgctc gattaaagaa ccggaaattg
    57241 ccgattgtgc aggcgcggaa gatgctccgc tgccgccttc cgaaccgccg caggccgcca
    57301 aaaccaatgc ggcaatgccg ccgagtacga attttttcaa catcattccc tcctgtaaaa
    57361 agtgtgcaag tgcggcattc taagtgaaat tgcggcaaac acaaagaata ctttttttat
    57421 atggttattc caaatcggaa tgggaaagat atgccgaagc cgccggaagc ggaaataaag
    57481 ggggcgggaa aggcggcggg cggcacgaaa ccgaaaagcg gcatcaggta tgcaaacggc
    57541 ggattcacgc acgccgttcc ggcaaaaatc cgcctgccga ttcggtgcta taatccaccc
    57601 gaaacagatg acaccaaaaa ggaacatctc atgaacacgc cccaatccgc catcattccc
    57661 gaccacgccc aagccggcat ctttatcgaa gccgacttcg ccgccaaccg cctcaacgat
    57721 attaaagccg cctgccgcgc ttcgctcgac gcgttatccg ccttgaaagc ccgctttccg
    57781 gacgatattt tgggtctgac catcgccttc ggcagcaaag cttgggcaac attcggacac
    57841 acggacgaag gcagtgaaat caaacccttc cccgaaatgg gcaacgggct tgcgccgtcc
    57901 acgcagcacg atatgtccat ccacatccaa tccttccgcc aaaacgccgc ctacgcgctt
    57961 gcccaatccg ttttgggcgc gttcggcgac agcatatgcg tcgcgtccga agaacacggt
    58021 ttgcgcctgt atcaggatcg cgggctggac ggtttcgtcg acggcacgga aaacccgcaa
    58081 ggcgatgaaa caatccgcga agtcgccatc atccccgaag ggctgcccga tgcaggcggc
    58141 agctatgtcc tgctgcaaaa atacctgcac gatttgaaaa aatgggatgc cgtccccgtc
    58201 gccgaacagg aggcctcggt cggacgcagc aaggaaaccg acgacgaatt cagccgcgat
    58261 gtccgcctgc ccgattcgca cctcggccgc gtcaacctga aagaaaacgg agtcggcctg
    58321 aaaatcgtcc gccgcagcct gcccttcggc aaaatcagcg gcgaacacgg tttgatgttt
    58381 accgcatact gccgcacgct gcataatatc gaagcgcaat tattgagtat gttcggcgac
    58441 acggacggca aaaccgacct gctgctccga cacctctccg ccgccgtttc gggcggatac
    58501 tactacgcgc cgtctgtcga acgcctgcaa aacctctaag ccggacgatg ccgtctgaaa
    58561 ccctttttca gacggcattt tcccgtttta ccgtccaacc gttttcatac acgctatgtc
    58621 cgcacccatc cgagcattcg acccgctgac cgcccccatt tccggcacca acctgattga
    58681 ggcctctgcc ggcaccggca aaacctacgg cattgccgcc ctgtttacgc gcctgatcgt
    58741 attggaacaa aaaagcgtcg agcgcgtatt ggtcgttacc ttcaccaaag ccgccactgc
    58801 cgagctgaaa acacgcctgc gcgcgcgttt ggacgatgtg ttgcaggttt tggaaagcaa
    58861 agaaattgcc gaacttggag acgacacgct ttcagacggc attgccgcct actgcgccga
    58921 acaccacgaa ggcgacacct tcctgcccgc actcttggaa caggctttgc aaaaagaaag
    58981 ccggacgcgt ctgattgtcc gcctcaaggc cgccatcggg caattcgaca acgccgccat
    59041 ctacaccatc cacggcttct gccagcgcat cctgcgcgac tacgccttcc tgtgccaagc
    59101 accgttcgat gtcgaactga ccgaagaaga cggcgaccgc ctgcttgtcc cggctcaaga
    59161 tttttggcgg gaacgcgtca gcggcgaccc ggtgcttgcc gcattggcgt ttaaacgcaa
    59221 agctgtgccg caaaccgtcc ttgcccaaat ccgcgcctac ctgtcccgcc cgtacctgaa
    59281 tttccgccgt ccgcaggcgg atttgaaaca ggcgcagcgc gacgccgaaa cctcatggca
    59341 aaccgtctgc cgcctgctgc ccgagctgga agccggcttt tggcgcatcc atcccgacct
    59401 caacggcaac agttaccgca aaaacagctt cggcaacctt tttaaagaac tggcacaaaa
    59461 atccgccgcc ggacaactgc cctttctgga caaagacacg cacgaacggc tcttgaaact
    59521 ctcatccgac aaactcgaag ccggactgaa aaaaggcaaa acgcccgatg cggcagtatt
    59581 tgccgaattg cagaaactgg cagacttcgg gcgcgatttg aacgcactcg aagaagcgga
    59641 agaaacaaca atgatccggc tgcaactgga tttaatcgaa tatctcaaca gcagccttgc
    59701 cgaaatgaaa aaatcgcgcc gcgaacgcgg tttcgacgac ctgctgctcg atgtccacac
    59761 cgcgctgacc gacaatccgc acgccgaaac ccccgcccgc gccgttgccg aaaactggga
    59821 aatcgcgctg atcgacgagt tccaagacac cgacccgctg caatacgaaa tcttccaaaa
    59881 gattttcatc gcccgaaacc gcccgctgtt cctcgtcggc gaccccaaac aggcgattta
    59941 cagctttcgc ggtgcggaca tttacgccta ccttcaggcg gcggaagacg cgcggcaccg
    60001 ctacacgctc gccaccaact accgcagcca cgccgcgctt atcggcagca tcggcgcgct
    60061 gttccgcctc aaagaacgcc cgttcgtttt ggaaaacatc ggctattcgg aagtcggtgc
    60121 ggcgcgtgcc gaaagcaggt tgtcgcccaa acgtcctgcc gtacagctcc gttggctgca
    60181 cgagaacgac aatgaaaaag ccaacaaaga cgttttgcgc cgccgtgccg ccgactattg
    60241 cgccgacgaa atcgcccgcg cgctcaacga agcctccgga ggccgtctga atttcaaagg
    60301 ccgcccgttg cagtcgggcg atattgccgt tctggtacgc acgcacaacg aggcggtgat
    60361 gatttccgcc gcactgaaaa aacggcaggt gcaaagcgtc ctgctttcgc gcgaatccgt
    60421 gttcgcctcg cccgaagccg ccgccctgtc cgcgctcatc ggcttctggc tcgaaccgcg
    60481 ccgcgccgga acgctgcgct ttgtcctgac aagcggcata ttcggttatg acgcgcagca
    60541 attgcacgac ttcaaccaaa acgaaagcga gattttgcat tgggcggaat ctgcccgaac
    60601 cgcgctcgac atatggcaaa aatacggcat tttcgccgct atgcagcagt tttcccaaac
    60661 acacggcatc gaaacgcgcc tcttaagccg gaacaacggg cgcagcctga ccaattattt
    60721 ccaactgctc gaactgcttg ccgccgaaga cgcgcaaaac cgcaaccccg ccgcactgca
    60781 caaatggctg cgcgatcaaa tcagcctggc cggcaacaac ggcggcgaca accgcgccat
    60841 ccgtttggaa agcgacgaag atttggtcaa aatcgttacc atgcacgcct cgaaaggttt
    60901 gcagtatccg ctggtgtact gcccgtttgc gtgggacgcg caagataccg gaccgtccga
    60961 ctggcaaatc ctccaccaag gcgcaaaccg ggccgaactt ttggcaaagg cgcaactgtc
    61021 ggaaagcgaa cagaagcaat acgccgacga agaaatggcg gaacgcctgc gcctgcttta
    61081 tgtcgcgctg acgcgtgccg aggaacagct caacatctac gccgcctatt cttccgatac
    61141 cgccgacaac ccccttgcct acctgatcga aggcttgccg caagacagcc gcgaaaccgt
    61201 ccgccgtacc tatgcgtgtg aaaaagacgg cataacgatg ctcaaacgca actggcggcg
    61261 cgtggcggac aatgccccgg ccggcacgga tttcgccttc acagaagatg cgccgccccc
    61321 tgccgcctat cgcggcaacg ccgatcaagc cgccgaattt gccgcaaaca gcattcccga
    61381 acgcggattc cgatttgtcc gccacaccag ctttaccgcc ttaagccgcc atacccaaac
    61441 gcccgacggc ggcgaagaag atgcctgccc gtccttggat gccgccgaaa cctcggtgcc
    61501 ggcgatgccg tctgaaacgc cgacggcttc agacggcata tcgatacacg actttccgaa
    61561 aggcacgcag gcggggctgt gcctgcacga aattcttgaa gatttcaaat tcggacaagc
    61621 agccgccgaa caggaaaccc tcattgccga caagctgaaa aaatacggtt ttgaagaaat
    61681 atggctgccc gccgttgccg aaatggcgga agcctgccgc aaaacgccgc tgactggggc
    61741 atacggcctg tccgacatcc cgcccgggtg ccgccgtccc gaaatgggct ttaccctcca
    61801 caccgaagac ttcggcctca aacggctgcg cgactggctt gcccgcgacg acatcaggct
    61861 gcccgaagtc tgccgtgccg ccgccgagac gctcgacttc cacaccgtca acggcttttt
    61921 aaacggcttt atcgatatgg tctgccaaga ccccgacggc aatatctgca tcatcgacta
    61981 caaatccaac cacctatccg cctacacgcg gcaggcgatg gacgaagccg tcgcgcacca
    62041 gcactattac cttcaggcac tgatttacgc cgttgccgcc gcgcgctact tcaaactgcg
    62101 cggacaaccg cccgccgccg tttccgtccg ctacctgttt ttgcgcggat tggatggcaa
    62161 aggcggcggc gtttggcgtt gggacataga tgccgccgct ttggaacaga taaaataact
    62221 tcccgtccct taagacgaca accgcaggag aaccctatga atatcaaaca attgattacc
    62281 gccgcactca ttgcctcagc cgcctttgcc acgcaggcag ccccgcaaaa acccgtatcc
    62341 gccgcccaaa ccgcgcaaca ttcagccgtt tggatcgatg tccgttccga acaggaattt
    62401 agcgaaggtc atttgcacaa cgcggtcaac atccccgtcg accaaatcgt ccgccgcata
    62461 tacgaagccg cgcccgacaa agacacgccg gtcaacctct actgccgcag cggacggcgt
    62521 gccgaagccg cccttcaaga gctgaaaaaa gcaggttata caaatgttgc caatcacggc
    62581 ggttatgaag acctgctcaa aaaagggatg aaatgacgca atgccgtctg aaaggacagg
    62641 ctttcagacg gcatacggcg ttccggcacg cggcacggca tcaggcgcgg cgcgtgccgc
    62701 cccgtccgaa gcccgataaa caaaccgata aggaaatacg atgaaacaca tactccccct
    62761 gatcgccgca tccgcactct gcatttcaac cgcttcggca caccctgccg acaaaccgcc
    62821 cacccaaaac gaaaccgcta tgaccacgca caccctcacc tcgaaataca gttttgacga
    62881 aaccgtcagc cgccttgaaa ccgccataaa aagcaaaggg atggacattt ttgccgtcat
    62941 cgaccatcag gaagcggcac gccgaaacgg cctgaccatg cagccggcaa aagtcatcgt
    63001 cttcggcacg cccaaggccg gtacgccgct gatggtcaaa gaccccgcct tcgccctgca
    63061 actgcccctg cgcgtcctcg ttaccgaaac ggacggcaaa gtacgcaccg cctataccga
    63121 tacgcgcgcc ctcatcgtcg gcagccgcat cagtttcgac gaagtggcaa acactttggc
    63181 aaacgccgaa aaactgatac aaaaaaccgt aggcgaataa gcccgtcgcc gacatatggc
    63241 ggatggaaat caaatgcaca cgcccctcat tcccccgaag tcgggaatcc aagcggttgg
    63301 gttaccgtga tttccaatca ttgccgaaac ggacgattcc ggattcccgc cttcgcaggg
    63361 atgacgggcg tatgcgtttt gccatatcac aggcaacttt gccgcaaatc ttatctgtcg
    63421 ccggtatgcc ttcttgtttc aatccgccac aaaccaaatg ccgtctgaaa cctgatttca
    63481 gacggcattt ccgtttattc cctgctgtcg cggcaaatat cgatggcttc ctgcaaactt
    63541 gaaacgccgt ggattttcag gttcggaaac tctttggcat tgcgcggcat attggctttg
    63601 gggacgatgg cgcgtttgaa gccgagcttt tccgcttctt tgagccgctc ttgtccgcgt
    63661 gcgacggggc ggacttcgcc gcttaggccg atttcaccga agaccacggt tttttcaggc
    63721 atagggcggt tgcggaagct ggaaagcatc gcgaggatga ccgccaaatc cgccgccggt
    63781 tcgccgattt tcacgccgcc gacggcgttt aaaaaaacat cctgatcgaa acaggcgatg
    63841 ccgccgtgcc ggtttaacac ggcaagcagc atcgcaaggc ggttttgttc caatccgacg
    63901 ctgaggcgtt tgggtgtgaa gccgtgcgcg tcatcgacca atgcctgaat ttcgaccaaa
    63961 agcgggcggc tgccttcctg tgtaaccaaa acgcacgaac cgggcgtgtc gtcgcggtag
    64021 ctggcgagga agatggcgga cgggttggat acgcctttca aaccgttttc ggtcatcgcg
    64081 aacacgccca attcgtttgc cgcgccgaaa cggtttttga tggcgcgtat catgcggtag
    64141 ttggaatgtt ggtcgccctc gaaatacagc acggtatcga ccatatgttc cagcacgcgc
    64201 gggccggcaa tcgctccgtc tttggtaacg tgtccgacca gtatcatggc gatacccatt
    64261 tgtttcgcca tacgcgtcag ttgggcggca cattcgcgca cctgcgacac ggaaccgggg
    64321 gcggacgtga tttggtcgga atacatggtt tggatggaat cgataacgac gacttcgggc
    64381 tgatgctgtt tcaaggccgc ctgaatcgct tccatgcgga tttcggcaag caggtttacg
    64441 ccttcggcgt gcagttccaa acgttgcgag cgcagggcga cctgttgggc ggattcttcg
    64501 ccggaaacgt acagcacttt gcggctttgc gccattttgg cgatggtttg caacaacagc
    64561 gtggatttgc cgatgccggg atcgccgccg agcaggatga ccgcgccatc gaccaaaccg
    64621 ccgcccaata cgcggtcgag ttcgcccata ccggtcggat tgcgcggcac ttcggcagcg
    64681 gtaacggcgg agagggattg gacggtcgaa gtgtccgccg cccaagattg gaatcgggcg
    64741 tttttcggct cgggcgcggc aaagctttcc tgaagcgtgt tccactcgcc gcaatgcgga
    64801 catttgccct gccatttcgg ggaagtgccg ccgcattcgg tgcattggta aagggtttta
    64861 agcgtttttg ccatgatgtt ttccctatgg gatttttgaa gtgtaaaaaa atcgtctgaa
    64921 acttcttttc taaaaaattt tttattatta gtttgttaat tttgattaca agaccatatc
    64981 acgatacaaa caagggtggc tttctgttat tttgcggcat aatgacggaa aatacaaaac
    65041 aggaatccca aacaatgact ttcccattaa atataatggt a
//
LOCUS       DBJGGI010000004        57041 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.004, whole genome shotgun sequence.
ACCESSION   DBJGGI010000004 DBJGGI010000000
VERSION     DBJGGI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 57041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57041
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.004"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(214..1494)
                     /gene="hemL"
                     /locus_tag="ACSIGJ_000235"
     CDS             complement(214..1494)
                     /gene="hemL"
                     /locus_tag="ACSIGJ_000235"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HHQ1329224.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAEI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGDEIACVIVEPFVGNMNLVRPTEA
                     FVKALRGLTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIQREGFYENLTALTQRLANG
                     IAAAKAHGIEFAADSVGGMFGLYFAAHVPRNYADMARSNIDAFKRFFHGMLDRGIAFG
                     PSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(1558..1943)
                     /locus_tag="ACSIGJ_000236"
                     /pseudo
     CDS             complement(1558..1943)
                     /locus_tag="ACSIGJ_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950978.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
     gene            complement(1994..2071)
                     /locus_tag="ACSIGJ_000237"
                     /pseudo
     CDS             complement(1994..2071)
                     /locus_tag="ACSIGJ_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
     gene            2268..3596
                     /gene="miaB"
                     /locus_tag="ACSIGJ_000238"
     CDS             2268..3596
                     /gene="miaB"
                     /locus_tag="ACSIGJ_000238"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HHQ1329225.1"
                     /translation="MKKVFIRTFGCQMNEYDSEKMLSVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKIVEA"
     gene            3614..3778
                     /locus_tag="ACSIGJ_000239"
     CDS             3614..3778
                     /locus_tag="ACSIGJ_000239"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329226.1"
                     /translation="MPPEAFQTAFCLVSADGTAAVIRTSGINFQTRNGTPSTARKPPP
                     EPFLPPAGFQ"
     gene            complement(3843..5756)
                     /gene="dxs"
                     /locus_tag="ACSIGJ_000240"
     CDS             complement(3843..5756)
                     /gene="dxs"
                     /locus_tag="ACSIGJ_000240"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HHQ1329227.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAATDKLLGGDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKEGGAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGEKTAFIAFGSMVATALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTEHGDPKKLLDDLGLSAEAVER
                     RVREWLPDRDAAN"
     gene            complement(5841..6758)
                     /gene="xerC"
                     /locus_tag="ACSIGJ_000241"
     CDS             complement(5841..6758)
                     /gene="xerC"
                     /locus_tag="ACSIGJ_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HHQ1329228.1"
                     /translation="MVLDGFAAYFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQDLSRRDFTAALRRLSQRGLDGRTLARKLSAWRQYCAWLVKRGLMRADP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLESWAAQYGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            6952..8016
                     /gene="fba"
                     /locus_tag="ACSIGJ_000242"
     CDS             6952..8016
                     /gene="fba"
                     /locus_tag="ACSIGJ_000242"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HHQ1329229.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLLEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAAGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLGKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            8155..9012
                     /locus_tag="ACSIGJ_000243"
     CDS             8155..9012
                     /locus_tag="ACSIGJ_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H-binding protein"
                     /protein_id="HHQ1329230.1"
                     /translation="MTRSKPVNRTTFCCLSLTAGPDSDRLQQRRGGGGGVAADIGTGL
                     ADALTAPLDHKDKGLKSLTLEASIPQNGTLTLSAQGAEKTFKAGGKDNSLNTGKLKND
                     KISRFDFVQKIEVDGQTITLASGEFQIYKQDHSAVVALRIEKINNPDKIDSLINQRSF
                     LVSDLGGEHTAFNQLPDGKAEYHGKAFSSDDAGGKLTYTIDFAAKQGHGKIEHLKTPE
                     QNVELASAELKADEKSHAVILGDTRYGGEEKGTYRLALFGGRAQEIAGSATVKIGEKV
                     HEIGIADKQ"
     gene            9151..9825
                     /gene="tsaB"
                     /locus_tag="ACSIGJ_000244"
     CDS             9151..9825
                     /gene="tsaB"
                     /locus_tag="ACSIGJ_000244"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HHQ1329231.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGEIRLFHQEVGIRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIALPEGYVFSDG
                     IGSAFALENRPPFSGKPDMPTAADFLALALGGYPATGAAHAELLYVRNKIALTAKEQA
                     ERKART"
     gene            9822..10262
                     /gene="rimI"
                     /locus_tag="ACSIGJ_000245"
     CDS             9822..10262
                     /gene="rimI"
                     /locus_tag="ACSIGJ_000245"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HHQ1329232.1"
                     /translation="MNIRPALPSDCAALAALDTVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGRIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWYAHLPEGTQRLLLE
                     VRAGNAAAQALYTKHGFSIAGRRKNYYCAADGQTEDAVLMEKIC"
     gene            10256..10993
                     /locus_tag="ACSIGJ_000246"
     CDS             10256..10993
                     /locus_tag="ACSIGJ_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HHQ1329233.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAATIRPSQPHNVQARLETMKALETAAVHTRKPAPETETPPPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGILLDNILKAAGLDAAYVHKTCWVKTAAVGNPMP
                     SEQAVADALGQIAGELDGCRAPAVLFLGQAFVNLERQAMIETLCGSRPFFIIDHPARL
                     LRQPELKARNWQVLKQLKRALRQGGGS"
     gene            11055..11696
                     /gene="pyrE"
                     /locus_tag="ACSIGJ_000247"
     CDS             11055..11696
                     /gene="pyrE"
                     /locus_tag="ACSIGJ_000247"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HHQ1329234.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGA
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DRGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRTYRRQYGVE"
     gene            11770..12198
                     /locus_tag="ACSIGJ_000248"
     CDS             11770..12198
                     /locus_tag="ACSIGJ_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HHQ1329235.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTHAISKKQISRDEIADILNGGTTLHDTPPATAAAA
                     PAAAPQVSVPPARQEGLNWTIATLFALIVLIMQLSYLFIL"
     gene            12195..13505
                     /gene="argA"
                     /locus_tag="ACSIGJ_000249"
     CDS             12195..13505
                     /gene="argA"
                     /locus_tag="ACSIGJ_000249"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004042 - L-glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HHQ1329236.1"
                     /translation="MNAPDSFVAHFREAAPYIRQMRGTTLVAGIDGRLLEGGTLNKLA
                     ADIGLLSQLGIRLVLIHGAYHFLDRLAAAQGRTPHYCRGLRVTDETSLGQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWMPPLGHSYGGKTFNLDMVQAAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAASETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNPHILVRRLHR"
     gene            13601..15616
                     /locus_tag="ACSIGJ_000250"
     CDS             13601..15616
                     /locus_tag="ACSIGJ_000250"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HHQ1329237.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLENDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQNAEYPKGVYRMCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLNKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPAYQIDKGAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSEGGAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADSKWQEAELPHLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRLQPQQFVSDGIEVRQFWAVSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGIPELPHYLGSVGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGMSSPKHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEACKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQRESADKLACVLLFLKEFLG"
     gene            complement(15729..16685)
                     /locus_tag="ACSIGJ_000251"
     CDS             complement(15729..16685)
                     /locus_tag="ACSIGJ_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHQ1329238.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLKM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDSGGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAGTLTAAACPLLQRPFESDGFGLLADEADALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILNSEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGRLRRLRLEHAL
                     ALIESGSTIQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            16921..17892
                     /locus_tag="ACSIGJ_000252"
     CDS             16921..17892
                     /locus_tag="ACSIGJ_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HHQ1329239.1"
                     /translation="MKPRFYWAACAVLPAACSPEPAAEKTVSAASQAASTPVATLTVP
                     TARGDAVVPKNPERVAVYDWAALDTLTEPGVNVGATTAPVRVDYLQPAFDKAATVGTL
                     FEPDCESLHRHNPQFVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLSR
                     IFGKEARVAELNAQIDALFAQKREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGD
                     IGLPPVDESLRNEGHGQPVSFEYIKEKNPGWIFIIDRTAAIGQEGPAAVEVLDNALVC
                     GTNAWKRKQIIVMPAANYIVAGGARQLIQAAEQLKAAFEKAEPVAAQ"
     gene            17925..18248
                     /locus_tag="ACSIGJ_000253"
     CDS             17925..18248
                     /locus_tag="ACSIGJ_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003768951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HHQ1329240.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGNMTIREGEMAVVPKSVSHRPRSENGCSVVLIDLPD
                     PSEAA"
     gene            18511..20688
                     /gene="tdfF"
                     /locus_tag="ACSIGJ_000254"
     CDS             18511..20688
                     /gene="tdfF"
                     /locus_tag="ACSIGJ_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent iron piracy receptor TdfF"
                     /protein_id="HHQ1329241.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPFGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     PDGTGEPSATVNLVRKHPTRKPLFEVRAEAGNRKHFGLGADVSGSLNAEGTLRGRLVS
                     TFGRGDSWRQLERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWSNSRNRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHSTAATDLIPGYWHADPRTHSASMSLTGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPSSFAQTIPQYDTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRAGSYNSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQLQ
                     KDEHGSYLKPVTGNNLEADIKGEWLEGRLNASAAVYRARKNNLATAAGRDQSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKPRDQDGSRLNPDSVPERSFKLFTAYH
                     LAPEAPSGRTIGAGVRRQGETHTDPAALHIPNPAAKARAVANSRQKAYAVADIMARYR
                     FNPRTELSLNVDNLFNKHYRTQPDRHSHGALRTVNAAFTYRFK"
     gene            complement(20951..21274)
                     /locus_tag="ACSIGJ_000255"
     CDS             complement(20951..21274)
                     /locus_tag="ACSIGJ_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HHQ1329242.1"
                     /translation="MDIVQLPSAALKAWMDEGRMFCLLDVRTDEEAAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLAEAGFENLYNLQGGIDAWAVEVDA
                     EMARY"
     gene            complement(21355..22011)
                     /locus_tag="ACSIGJ_000256"
     CDS             complement(21355..22011)
                     /locus_tag="ACSIGJ_000256"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase
                     family protein"
                     /protein_id="HHQ1329243.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAERQKRAKAVLDGLSLENIDYVQNNGLTELSAGAPFDAVYVGGAVTLVPEVL
                     KEQLKDGGRMAVIVGRRPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDAHPFDSFNF
                     "
     gene            complement(22113..22640)
                     /locus_tag="ACSIGJ_000257"
     CDS             complement(22113..22640)
                     /locus_tag="ACSIGJ_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HHQ1329244.1"
                     /translation="MYATIGDVMHALHFSASDKAALYREVLPQIESVVADETDWVANL
                     ANTAAVLKEAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGT
                     VVVGDVDAHPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGEL
                     AKILEKRFEASRQAA"
     gene            22800..23573
                     /gene="tpiA"
                     /locus_tag="ACSIGJ_000258"
     CDS             22800..23573
                     /gene="tpiA"
                     /locus_tag="ACSIGJ_000258"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HHQ1329245.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            23580..23930
                     /gene="secG"
                     /locus_tag="ACSIGJ_000259"
     CDS             23580..23930
                     /gene="secG"
                     /locus_tag="ACSIGJ_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HHQ1329246.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            23944..24029
                     /locus_tag="ACSIGJ_000260"
     tRNA            23944..24029
                     /locus_tag="ACSIGJ_000260"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23978..23980,aa:Leu,seq:gag)
     gene            24831..25199
                     /locus_tag="ACSIGJ_000261"
     CDS             24831..25199
                     /locus_tag="ACSIGJ_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HHQ1329247.1"
                     /translation="MRIFKNQWIVKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIEL"
     gene            25196..25507
                     /locus_tag="ACSIGJ_000262"
     CDS             25196..25507
                     /locus_tag="ACSIGJ_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHQ1329248.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(25476..25730)
                     /locus_tag="ACSIGJ_000263"
     CDS             complement(25476..25730)
                     /locus_tag="ACSIGJ_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329249.1"
                     /translation="MPRGRGCFGFRARLRRALLSGYAVCTAAVSAWFDAVGHLCGYAL
                     IFGSAVLPVLAGIFSSASIVNLLILPFAKSATQWLRYPCF"
     gene            complement(26277..26832)
                     /locus_tag="ACSIGJ_000264"
                     /pseudo
     CDS             complement(26277..26832)
                     /locus_tag="ACSIGJ_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26829..27637)
                     /locus_tag="ACSIGJ_000265"
                     /pseudo
     CDS             complement(26829..27637)
                     /locus_tag="ACSIGJ_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751586.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-methyltransferase"
     gene            complement(27630..27800)
                     /locus_tag="ACSIGJ_000266"
                     /pseudo
     CDS             complement(27630..27800)
                     /locus_tag="ACSIGJ_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(27767..28558)
                     /locus_tag="ACSIGJ_000267"
     CDS             complement(27767..28558)
                     /locus_tag="ACSIGJ_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HHQ1329250.1"
                     /translation="MNLFFDTQLGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPHHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKDWLLAVMKCIDQLTEEFTLSQMYEFENKLSIQ
                     FPQNNHIKDKIRQQLQILRDQNMIEFIGRGLYKKIDKLHPTPKAF"
     gene            complement(28787..31417)
                     /gene="leuS"
                     /locus_tag="ACSIGJ_000268"
     CDS             complement(28787..31417)
                     /gene="leuS"
                     /locus_tag="ACSIGJ_000268"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HHQ1329251.1"
                     /translation="MQEHYQPAAIEPAAQKKWDDARISNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAVDWEREVATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYNLPKKQVIAVGDNAFDANRWQEWYGDKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     KCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETSCPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAESAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGGAVKAFAGNQDGLSKELKDLRHKLHSTTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGSEQGRAVAQEVLEAAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALANEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            31684..32200
                     /locus_tag="ACSIGJ_000269"
                     /pseudo
     CDS             31684..32200
                     /locus_tag="ACSIGJ_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:1990063 - Bam protein complex [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
     gene            32207..32650
                     /locus_tag="ACSIGJ_000270"
     CDS             32207..32650
                     /locus_tag="ACSIGJ_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HHQ1329252.1"
                     /translation="MPPRLLSGILCCLLTTAPVFAQGQPDAVSAYIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGTATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            32819..34876
                     /gene="ppk1"
                     /locus_tag="ACSIGJ_000271"
     CDS             32819..34876
                     /gene="ppk1"
                     /locus_tag="ACSIGJ_000271"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HHQ1329253.1"
                     /translation="MTEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRPDNGKTPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDVQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFRLTDAELYQVKGPVNLVRLNAVPDLVNRPDLKFPPHTPGRLKALGKNSPIFD
                     LVRQSPILLHHPYQSFDPVVDMIREAAADPAVLAVKMTIYRTGTRSELVPALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGLITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIGRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIVGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     ATPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWDL
                     LRG"
     gene            complement(35025..36128)
                     /gene="dnaN"
                     /locus_tag="ACSIGJ_000272"
     CDS             complement(35025..36128)
                     /gene="dnaN"
                     /locus_tag="ACSIGJ_000272"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HHQ1329254.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGRGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDNRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLGQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(36208..37764)
                     /gene="dnaA"
                     /locus_tag="ACSIGJ_000273"
     CDS             complement(36208..37764)
                     /gene="dnaA"
                     /locus_tag="ACSIGJ_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HHQ1329255.1"
                     /translation="MTLAEFWPLCLRRLHDMLPHGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRPAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEKPVKPAASKTAADILAERMKNLPHEPRQAAGPASRPESAA
                     VAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAENPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            38056..38190
                     /gene="rpmH"
                     /locus_tag="ACSIGJ_000274"
     CDS             38056..38190
                     /gene="rpmH"
                     /locus_tag="ACSIGJ_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HHQ1329256.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            38193..38558
                     /gene="rnpA"
                     /locus_tag="ACSIGJ_000275"
     CDS             38193..38558
                     /gene="rnpA"
                     /locus_tag="ACSIGJ_000275"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HHQ1329257.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCGKQV"
     gene            38623..38844
                     /gene="yidD"
                     /locus_tag="ACSIGJ_000276"
     CDS             38623..38844
                     /gene="yidD"
                     /locus_tag="ACSIGJ_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HHQ1329258.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGLRLAIKRIARCHPFGGHGHDPVP"
     gene            39017..40654
                     /gene="yidC"
                     /locus_tag="ACSIGJ_000277"
     CDS             39017..40654
                     /gene="yidC"
                     /locus_tag="ACSIGJ_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HHQ1329259.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQKQAATA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFVLFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYTLNGDTVEVRLSAPETNGLKIDKVYTFTKD
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCHIDI
                     KRRSDKLYSASVSVPLAAIQAGAKAETAVNLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQT
                     IKEKYGDDRMAQQQAMMQLYKDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(40735..41445)
                     /locus_tag="ACSIGJ_000278"
     CDS             complement(40735..41445)
                     /locus_tag="ACSIGJ_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HHQ1329260.1"
                     /translation="MSPVLYLIPTPLGTPDTPCLLQHEQRAVVGLTDFVVEAEKTARA
                     HLKHLGITTPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGAN
                     LVALAHKHGFEVRPLVGPSSLLLALMASGANGQNFAFNGYLPSEKNERIQSLNALEQR
                     SRQCGETQIFIETPYRNDALLADAVENLHPETRLCTATDLTLPTQLVVSKTVADWRRM
                     KEMPNLKKRPTIFVMYAG"
     gene            complement(41507..42097)
                     /locus_tag="ACSIGJ_000279"
     CDS             complement(41507..42097)
                     /locus_tag="ACSIGJ_000279"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HHQ1329261.1"
                     /translation="MGLELPLVLGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPGALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMHRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCAAKSEGL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVDVL"
     gene            42245..42745
                     /locus_tag="ACSIGJ_000280"
     CDS             42245..42745
                     /locus_tag="ACSIGJ_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HHQ1329262.1"
                     /translation="MSDPNLIDPEIFAAERQNLQGSFLLEELDERVSLHDYPADRRTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSDEESLDES
                     MLADEELEGILIEKELDVRALVEDQILMSLPFSPRHGHCGNTLPESANQDKPNPFAVL
                     AGLKSS"
     gene            42779..42958
                     /gene="rpmF"
                     /locus_tag="ACSIGJ_000281"
     CDS             42779..42958
                     /gene="rpmF"
                     /locus_tag="ACSIGJ_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HHQ1329263.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALFVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            43133..43660
                     /locus_tag="ACSIGJ_000282"
     CDS             43133..43660
                     /locus_tag="ACSIGJ_000282"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106130 - purine phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HHQ1329264.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNAGVKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEVLRGKNVLVVDEVDDS
                     RVTMEFCLKELLKEDFDTVGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            43750..44796
                     /gene="plsX"
                     /locus_tag="ACSIGJ_000283"
     CDS             43750..44796
                     /gene="plsX"
                     /locus_tag="ACSIGJ_000283"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HHQ1329265.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALNAAGAPMERIDICHTTQVVGMDESPQSALKNKKYSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDKTGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALEAAQNETAASL"
     gene            44934..45185
                     /locus_tag="ACSIGJ_000284"
     CDS             44934..45185
                     /locus_tag="ACSIGJ_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HHQ1329266.1"
                     /translation="MKTLEKRMKALDKRMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFDILCTYIEDFDIRLTEQESREISFINKEFEIESTSD"
     gene            45212..45427
                     /locus_tag="ACSIGJ_000285"
                     /pseudo
     CDS             45212..45427
                     /locus_tag="ACSIGJ_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6911 family protein"
     gene            45560..45748
                     /locus_tag="ACSIGJ_000286"
                     /pseudo
     CDS             45560..45748
                     /locus_tag="ACSIGJ_000286"
                     /inference="COORDINATES: protein motif:HMM:NF046131.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6911 family protein"
     gene            45802..46005
                     /locus_tag="ACSIGJ_000287"
     CDS             45802..46005
                     /locus_tag="ACSIGJ_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329267.1"
                     /translation="MPSEAAWAFRRHFLHLKQRIGNPAPPVHGNPHLKTICMPNHTPI
                     PVSVICTLPVLGNHRFFAYNSRI"
     gene            46032..46994
                     /locus_tag="ACSIGJ_000288"
     CDS             46032..46994
                     /locus_tag="ACSIGJ_000288"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HHQ1329268.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALADAKLNINDIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAEQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDAGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            47041..47412
                     /locus_tag="ACSIGJ_000289"
     CDS             47041..47412
                     /locus_tag="ACSIGJ_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HHQ1329269.1"
                     /translation="MKRQAFFKPMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWTGAYLGSRMFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            47438..48364
                     /gene="fabD"
                     /locus_tag="ACSIGJ_000290"
     CDS             47438..48364
                     /gene="fabD"
                     /locus_tag="ACSIGJ_000290"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HHQ1329270.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEVGGKTPAAVAGHSLGEYTALVAAGA
                     LDFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVRQICAESAQGEVVEAVNFN
                     SPGQVVIAGNAAAVGRTMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAETLKTVE
                     IKQPQIRVIHNADVAAYDDAGKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQITAFIEAH"
     gene            48503..50371
                     /gene="msbA"
                     /locus_tag="ACSIGJ_000291"
     CDS             48503..50371
                     /gene="msbA"
                     /locus_tag="ACSIGJ_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HHQ1329271.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAADILSTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTALTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGESEVLSALQAANLQSLIDASPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTVESADRIIVMDGGKIIEQGTHDQLMFQNGYYTMLRNISGKDTAAVQTA"
     gene            50482..52047
                     /gene="guaA"
                     /locus_tag="ACSIGJ_000292"
     CDS             50482..52047
                     /gene="guaA"
                     /locus_tag="ACSIGJ_000292"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HHQ1329272.1"
                     /translation="MTQDKILILDFGSQVTRLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSGLTRGIQDDAPNTLDVWMSHGDKVSKLPDGFAVIGDTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            52109..52855
                     /gene="fabG"
                     /locus_tag="ACSIGJ_000293"
     CDS             52109..52855
                     /gene="fabG"
                     /locus_tag="ACSIGJ_000293"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HHQ1329273.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKIIGTATGESG
                     AAAISKRLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFAKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
     gene            52920..53405
                     /locus_tag="ACSIGJ_000294"
     CDS             52920..53405
                     /locus_tag="ACSIGJ_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HHQ1329274.1"
                     /translation="MNTHHHPPCPCGSGTSYAGCCRPLHLRQILPPTAEALIRSRYGA
                     YVLHLIDYIIATTVPAQQTFLDAAELMQWSREAEWLGLNVIAHRNLGKQHAQVEFEAY
                     FQDGGNRTVHHELSAFVKIAEQWYFIDPTVPLPTMKQPCICGSGKKFKACCGKYLQPV
                     A"
     gene            complement(53624..54418)
                     /locus_tag="ACSIGJ_000295"
     CDS             complement(53624..54418)
                     /locus_tag="ACSIGJ_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HHQ1329275.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSRRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            54522..55247
                     /locus_tag="ACSIGJ_000296"
     CDS             54522..55247
                     /locus_tag="ACSIGJ_000296"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HHQ1329276.1"
                     /translation="MPRFYLPENLSVGQTVDLPDNIVRHLNVLRIRPNENITLFDGKG
                     KAHTARLTVLEKHRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTRLIMSINRACKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAAIAAMQTLWGDFT"
     gene            complement(55443..56285)
                     /locus_tag="ACSIGJ_000297"
     CDS             complement(55443..56285)
                     /locus_tag="ACSIGJ_000297"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901271 - lipooligosaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HHQ1329277.1"
                     /translation="MQNHVISLASAAERRAHIADTFGSRGIPFQFFDALMPSERLERA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWEQALDEGLPYIAVFEDDVLLGEGAEQFLA
                     EDTWLQERFDPDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHCGTAGYIISR
                     EAMRFFLDRFAVLPPERIKAVDLMMFTYFFDKEGMPVYQVSPALCIQELHYAKFLSQN
                     SMLGSDLEKDREQGRRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKVYGRRV
                     ILFK"
     gene            complement(56304..>57041)
                     /locus_tag="ACSIGJ_000298"
     CDS             complement(56304..>57041)
                     /locus_tag="ACSIGJ_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HHQ1329278.1"
                     /translation="RTDADDIASPGWIEKIVGEMEKDRSIIAMGAWLEVLSEENNKSV
                     LAAIARNGAIWDKPTRHEDIVAVFPFGNPIHNNTMIMRRSVIDGGLRFDPAYIHAEDY
                     KFWYEAGKLGRLAYYPEALVKYRLHQDQTSSKYNLQQRRTAWKIKEEIRAGYWKAAGI
                     AVGADCLNYGLLKSTAYALYEKALSGQDIGCLRLFLYEYFLSLEKYSLTDLLDFLTDR
                     VMRKLFAAPQYRKILKKMLRPWKYRSY"
BASE COUNT        13724 a        17168 c        14306 g        11843 t
ORIGIN      
        1 cagacggcat ttttacagta gattgaaatc aaaaatgtac acgcccgtca ttcccgcgca
       61 ggcgggaatc cggaaggtcg ggcttgccgt tattttcaat cattacagaa actgaaaggt
      121 ccggattccc gcctgcgcgg aaatgacggc ggtgtgcatt cttacttcaa tctgccaaac
      181 aaatcgaaca gagaaactct gtccgtcaaa acatcatgca gccatcgcct tgaacacttc
      241 aaccgcaacc gcaaccgttt cgtcaatcag ctcgggcgta tgcgcggcgg aaacgaaacc
      301 tgcttcataa gcggacgggc cgaaggcaat gccgcggtcg agcatgccgt ggaagaagcg
      361 tttgaaagcg tcgatattgg agcgcgccat atcggcatag tttcgcggca cgtgtgcggc
      421 gaaatacaga ccgaacatac cgcccacgct gtcggcggca aactcgatac cgtgcgcttt
      481 ggcggcggca ataccgttgg caaggcgttg tgtcaaggcg gtcaggtttt catagaagcc
      541 ttcgcgctgg atgatttcca gcgttttcaa gccggcggcg acggcaatcg ggttgcctga
      601 taatgtacct gcctgataca cgccgcccaa cggggaaata cattccatga tgtctttgcg
      661 tccgccgaac gcggcaagcg gcataccgcc gccgatgact ttgcccatcg tggtcaggtc
      721 gggcgtgatg ccgtgcagcg actgcgcgcc gccgagcgcg acgcggaaac cggtcatcac
      781 ttcgtcgtaa atcaacaccg cgccgtgttt ttcggtcaat ccgcgcaagg ctttgacaaa
      841 ggcttcggtc gggcggacga ggttcatatt gccgacgaag ggttcgacaa tcacgcaggc
      901 gatttcgtcg ccgctttggg caaaggcttc ttcgagttgg gcgatgttgt tgtattcgag
      961 taccaaagta tgtttggtaa agtcggcagg cacaccggcg gaagaaggat tgccgaaggt
     1021 aagcagaccg ctgcctgctt tcaccaacag gctgtcggaa tggccgtggt agcagccttc
     1081 aaatttgatg attttgtcgc ggccggtaaa accgcgtgcc agacggatgg cagtcatcgt
     1141 cgcttccgtg ccggagctga cgaggcgcag ccgttcgaca gacggcataa tttcggcaat
     1201 ttgttcggca atggcgattt cgccttcggt gggcgcgccg aacgacaaac cgcccaacgc
     1261 agcttcgcgc acggcttcga cgacttcggg atgcgcgtgt ccgacaatcg caggccccca
     1321 agagccgaca taatcggtgt agcgcgtgcc gttttcgtcc caaacatacg cgccttcggc
     1381 ttttttgatg aagcgtggca cgccgccgac gctgccgaat gcgcgcacgg gcgaattcac
     1441 gccgccgggg atgatggctt tggcgcggtc gaataaaatt tcgttacggt tcatatttat
     1501 cctcaaatgc cgtctgaacg gcaggtttcg ggcttggaag cagaaagccc cattttatca
     1561 tttttcaggt tgcgacaagg atttgcccgc ttctttgcgg attacgccaa ccgtatcccg
     1621 gatgacggaa cgctcgtctt tttcctcttt atatgtgtaa agcggtagtc ccggacgact
     1681 ccctccccgt cgtaatccac gcaccattcc caatgccgcc gctccgattt catatagatg
     1741 aaattggtcg gcaaaaaatt ataaatcggc aggctgacct cgtgataggc ataacagccg
     1801 aaagggttgc gcttcccgaa acgtgcctct acctccgccc gggtcgtttt gcctttaaca
     1861 accgtttgtg cgattccctc ttccgtctga tatagtggat taacaaaaat caggacaagg
     1921 cgacgaagcc gcagacagta caaatagtac ggcaaggcga ggcaacgctg tactggttta
     1981 aatttaatcc actataacgc aggaactgat gttccctgtc gccgaaattg ctggtacacg
     2041 cacacagcag caatgccgcc catacacaca cctcccgtta aagccaaaca ttatacagcc
     2101 gttccgaccg attaaattca tattttaaaa caatattttg cctcccaaac ccacatcgtg
     2161 ctataatccg caccgatttt cagacggcat cgtcgtgccg tctgaaattt tttcattcca
     2221 acaaccatca gccccgcgat tacggctgcc cgagaaagac acaaaccatg aaaaaagtat
     2281 ttatccgcac cttcggctgc cagatgaacg agtacgacag cgaaaaaatg ctgtccgtcc
     2341 ttgccgaaga acacggcggc atcgaacagg ttacccaagc cgacgaagcc gacatcatct
     2401 tgttcaacac ctgttccgtg cgcgaaaaag cacaggaaaa agtgttctcc gacttggggc
     2461 gcgtccgtcc gctcaaagaa aaaaaccccg gcctcatcat cggcgttgcc ggctgcgtcg
     2521 cctcgcaaga aggcgaaaac atcatcaaac gcgcgcctta tgttgacgtg gttttcggcc
     2581 cgcaaacgct gcaccgcctg cccaaaatga ttgtggacaa agaaaccagc gggctgtcgc
     2641 aagtcgatat ttccttcccc gaaatcgaaa aattcgacca cctgccgccc gcccgcgtcg
     2701 aaggcggcgc ggcatttgtg tcgattatgg aaggctgttc caaatactgc tccttctgcg
     2761 tcgtccccta cacgcgcggc gaagaattct cccgcccgct caacgacgta ttgaccgaaa
     2821 tcgccaacct tgcccagcaa ggcgtgaaag aaatcaacct cttgggacaa aacgtcaatg
     2881 cctatcgcgg cgaaatggac gacggcgaaa tctgcgactt cgccaccctg ctgcgtatcg
     2941 tccacgaaat ccccggcatc gaacgtatgc gcttcaccac cagccacccg cgcgagttta
     3001 ccgactcgat tatcgagtgc taccgcgacc tgcccaaact ggtttcccac ctgcacctgc
     3061 cgattcaaag cggttccgac cgcgtattga gcgcgatgaa acgcggctac accgctttgg
     3121 aatacaaatc catcatccgc aaactgcgcg ccatccgtcc tgatttgtgc ctgagcagcg
     3181 acttcatcgt cggtttcccc ggcgagaccg aacgcgagtt cgagcaaacc ttgaaactgg
     3241 tgaaagacat tgccttcgac ttgagcttcg tgtttattta cagcccgcgc cccggcacgc
     3301 ctgccgccaa cctgccggac gacacgccgc acgaagaaaa agtgcgccgc ctcgaagcct
     3361 tgaacgaagt catcgaagcc gaaaccgccc gcatcaacca aaccatgatc ggcacggttc
     3421 aacgctgcct ggtcgaaggc atctccaaaa aagaccccga ccagctccaa gcccgtaccg
     3481 ccaacaaccg cgtcgtcaac tttaccggca cgcccgacat gatcaaccaa atgatcgatt
     3541 tggaaatcac cgaagcctac accttttccc tgcgcggcaa aattgtcgaa gcctaaaacc
     3601 ctcacgccga aaaatgccgc ctgaagcgtt tcagacggca ttttgccttg tatcggcaga
     3661 cggcaccgcc gccgttatac ggacaagcgg aataaatttc caaacgcgca acggcacacc
     3721 aagcaccgcc cgaaagccgc cgcccgaacc cttcctgccg cctgcggggt ttcaataaaa
     3781 aaaccgcaga aacaaacgcc gtttaaaaac gccgcccgat aacgacggcg cggtcaagcg
     3841 gtttaatttg ccgcatcacg gtccggcagc cactcgcgca cccggcgttc caccgcttcg
     3901 gcactcaaac ccaaatcgtc caaaagtttt ttcggatcgc cgtgttcggt tacggtatcg
     3961 gcaacgccca aaagcaaaac gggtttgcag atgccgtgtt tcgccaacac ttccaagacc
     4021 gcgccgcctg cgccgccctg ttcggcgttt tcttcaaggg taacgatgcg gtcgtggctt
     4081 cgggcaaggc ggacaatcaa ctcttcgtct atcggtttga cgaagcgcat atcggcgacg
     4141 gtggcgttca gtttttcggc aaccgccaat gcggtggcga ccatactgcc gaaggcaatg
     4201 aaggcggttt tctcaccttc gcggcggata atgcccttgc cgatttccac ggtttccatg
     4261 ccgtctgaaa ccggcgcgcc cgtacccgtg ccgcgcggat agcggacggc ggcgggcgca
     4321 tccgcctgat agcaggtcga aagcagcagg cggcattcgt tttcatcgct cggcgcggca
     4381 acaatcatgt tcggcacaca gcgcaagaag ctcaaatcgt acaagccggc atgggtcggg
     4441 ccgtccgcgc cgacgatgcc cgcacggtcg acggcaaaca aaacgggcag gttttgcagg
     4501 gcgatgtcgt gcaccagttg gtcgtaggcg cgttgtaaaa aggtggaata aatcgccacg
     4561 acgggcttca tgccttcgca cgccaaaccg ccggcaaagg taacggcgtg ctgctcggcg
     4621 atgccgacat cgaaatagcg gtcggggaat cgttgttcaa actccaccag tccgctgccc
     4681 tcgcgcatgg cgggggtaat cgcaaccagt cgggaatctg ccgccgcccg gtcgcacagc
     4741 catttgccga atacttgggt ataggtcggt ttggcggcgg gcttgggttc tttttcagac
     4801 ggcatttgcg ccccgccttc tttaggcagg ttggcgacgg cgtggtattt gacggggtcg
     4861 ttttcggcga gtttgtagcc gttgcccttt ttggtgatga cgtgcagcaa ctgagggcct
     4921 ttgcggctgc gcaagtcttt caatacgtcc accagattct cgacgttgtg tccgtccacg
     4981 gggccggtgt agcggaagcc gaaattttca aacagcgaca gcgactgttt ggcgtgttcg
     5041 gcttcttcgg caagggtttt gattttgtgt tcgacttttt gggcaaactc catcgcgccg
     5101 ggtattttgt ctaatacctt gcccgtttgc gctttgacgg tactcaacag tccgtgcata
     5161 tcgcgcacga cgttgctggc aagatatttg ggcaacgcgc cgacgttggg ggaaatcgac
     5221 atttcgttgt cgttgaggac gaccagcaaa tccacatcca tatcgcccgc gcaattcaag
     5281 gcttcaaacg cctgccccgc cgtcatcgcg ccgtctccga tgatggcgac gctgcggcgg
     5341 tcgccgccca agagtttgtc cgtcgccgcc atgcccaaag ccgcgccgat ggaggtggag
     5401 gaatgcccca cgccgaacgc gtcgtactcg gactcgcaac gtttcggaaa acccgccaaa
     5461 ccgccgtatt ggcgcatggt gtgcatctgg tttttcctgc ctgtcaggat tttgtgcggg
     5521 tagctttggt gtccgacatc ccacaccagc ttgtcttcgg gcgtgtcgta cacatagtgc
     5581 agggcgatgg tcagttcgac cgcacccaga ttgctggcga aatgcccgcc ggtctgcccg
     5641 acagattcca gcagaaaggc gcgcaactcg ccggcaaggc gcggcagctg ttttttgtcc
     5701 aggcggcgca aatcttgcgg gctgtcaatc aggtcgagta gggggcttgg gttcatggtg
     5761 tgtctttttt atgtgtcgtc cgggtgcaac ggtcaattat atatcaagag cgtgcggctg
     5821 acggctgatt ttgccgtacg tcattcgtcc tgccgcttgg cgcgcgggtg ggcttcgtca
     5881 tagaggcggg cgatgtggtc gaaatcgagc ttggtataaa tctgcgtggt cgaaaggctg
     5941 ctgtgtccga gcagctcctg caccgccctg atgtcgcgcg aagcctgcaa caggtggccg
     6001 gcgtagctgt ggcgcatcat atgcggcgaa acgtgcctgc cgtcgccgta ttgcgccgcc
     6061 cacgattcga ggcgtttttg gatttggcgt tggctcaggc gcgtgccgtt cctgccggta
     6121 aacagggctt tgccgtccga tgccgtctga cgcagcggca gatagttttt caaggcttcc
     6181 acgcttttgc cggtcagcgg aacctgacgc tgcttgcgcc ctttgccggt aacgtgtacc
     6241 cacgcttcgt ccaaatatac atcatctgca ttcaagccgt gtatctcgct cacgcgcaaa
     6301 ccgctgccgt acatcagctc gaacagcgcg tggtcgcgca ccgccagcgg gtcgccgccg
     6361 tccacgggca aatccaacat ccggttcagc cattcctgcg gcagggcttt gggtacgcgc
     6421 tcgggctgct tcggcggttt gatgtcggcg gtcgggtcgg cgcgcatcag cccgcgtttg
     6481 accagccagg cgcaatactg ccgccacgcc gacagcttgc gcgccagcgt ccgaccgtcc
     6541 aaaccgcgct gcgacagccg ccgcaacgcc gccgtaaaat cgcgccgcga caagtcctgc
     6601 ggcacgccgc ctgcatcttc agacggcatt tgtgccaaca gtgcaaacag ttcttccaaa
     6661 tcgcgccggt atgccgcaac cgtgtgctcc gacttgccct cgcgcacgat gttttccaaa
     6721 taagcgtcaa aatacgccgc aaacccgtcc aaaaccataa ccgtcccaca caaatatcaa
     6781 aaaaccagtg aaaacattat cataaacaac acaccaaaat cccgaaccgc ccaaaatccg
     6841 ccccatctgc ccgccgttat cgttaatata aaatcaatga gccgtttatg gttttttgcc
     6901 gtaaaaaaca ttataatccg ccttatttac ctattgccca aggagacaca aatggcactc
     6961 gtatccatgc gccaactgct tgaccacgcc gccgaaaaca gctacggcct gcccgcgttc
     7021 aacgtcaaca acctcgaaca aatgcgcgcc attatggaag ccgccgacca agtcaacgcg
     7081 cccgtcatcg tacaggcgag cgcaggtgcg cgcaaatacg cgggcgcgcc gtttttgcgc
     7141 cacctgattc tggcggcagt cgaagaattt ccgcacatcc ccgtcgtgat gcaccaagac
     7201 cacggcgcat cgcccgacgt gtgccaacgc tccatccaac tgggcttctc ctccgtgatg
     7261 atggacggct ctttgctcga agacggcaaa accccttctt cttacgaata caacgtcaac
     7321 gccacccgta ccgtcgtcaa cttctcccac gcctgcggcg tgtccgtcga aggcgaaatc
     7381 ggcgtattgg gcaacctcga aaccggcgaa gcaggcgaag aagacggagt gggcgcggca
     7441 ggcaaactct cacacgacca aatgctcacc agcgttgaag atgccgtgcg tttcgttaaa
     7501 gataccggcg ttgacgcatt ggcgattgcc gtcggcacca gccacggcgc atacaaattc
     7561 acccgtccgc ccacaggcga cgtattgcgt atcgaccgca tcaaggaaat ccaccaagcc
     7621 ctgcccaata cacacatcgt gatgcacggc tccagctccg ttccgcaaga atggctgaaa
     7681 gtcatcaacg aatacggcgg caatatcggc gaaacctacg gcgtgccggt tgaagaaatc
     7741 gtcgaaggca tcaaacacgg cgtgcgcaaa gtcaacatcg ataccgacct gcgcctcgct
     7801 tccaccggcg cggtacgccg ctaccttgcc gaaaacccgt ccgactttga tccgcgcaaa
     7861 tacttgggca aaaccattga agcgatgaag caaatctgcc tcgaccgtta tcttgcgttc
     7921 ggttgcgaag gtcaggcagg caaaatcaaa cctgtttcgt tggaaaaaat ggcaagccgt
     7981 tatgccaagg gcgaattgaa ccaaatcgtc aaataacagg ttgcctgtaa acaaaatgcc
     8041 gtctgaacgc cgttcggacg gcatttgatt tttgcttctc tgacctgcct cattgatgca
     8101 gtatgcaaaa aaagatacca taaccaaaac gtttatatat tatctattct gtgtatgact
     8161 aggagtaaac ctgtgaaccg aactaccttc tgctgccttt ctttgaccgc cggccctgat
     8221 tctgaccgcc tgcagcagcg gaggggcgga ggcggtggtg tcgccgccga catcggcacg
     8281 gggcttgccg atgcattaac cgcgccgctc gaccataaag acaaaggttt gaaatcccta
     8341 acattggaag cctccattcc ccaaaacgga acactgaccc tgtcggcaca aggtgcggaa
     8401 aaaactttca aagccggcgg caaagacaac agcctcaaca cgggcaaact gaagaacgac
     8461 aaaatcagcc gcttcgactt cgtgcaaaaa atcgaagtgg acggacaaac catcacactg
     8521 gcaagcggcg aatttcaaat atacaaacag gatcactccg ccgtcgttgc cctacggatt
     8581 gaaaaaatca acaaccccga caaaatcgac agcctgataa accaacgctc cttccttgtc
     8641 agcgatttgg gcggagaaca taccgccttc aaccaactgc ctgacggcaa agccgagtat
     8701 cacggcaaag cattcagctc cgacgatgcc ggcggaaaac tgacctatac catagatttc
     8761 gccgccaaac agggacacgg caaaatcgaa cacctgaaaa cacccgagca gaatgttgag
     8821 cttgcctccg ccgaactcaa agcagatgaa aaatcacacg ccgtcatttt gggcgacacg
     8881 cgctacggcg gcgaagagaa aggcacttac cgcctcgccc ttttcggcgg ccgcgcccaa
     8941 gaaatcgctg gctcggcaac cgtgaagata ggggaaaagg ttcacgaaat cggcatcgcc
     9001 gacaaacagt agtatttgaa aaatgccgtc cgaacacgat aatttaccgt tcggacggca
     9061 tttttgcgcc ataccgccct aggacacgac gacacggcag gcaagccgat ttcactatat
     9121 aatgccgtct gaaccaacga gaaccacacc atgcaagccg atttcaaccg ccccgtcctc
     9181 gccgtcgata ccggaacttc ctatttgtcg ctcgcgctgc gcgccgacgg cgaaatccgt
     9241 ctgttccatc aggaagtcgg catccgccag tccgaactga tcctgccgga aatccgcacc
     9301 ctattccgaa atgcgggcat taccgccgcc gatttggggg cgattgttta tgcgaaaggc
     9361 cccggcgcgt ttaccggact gcgtatcggc atcggcgtgg cgcaaggttt ggcaacgccg
     9421 ttcgacacgc ctttaatcgg catccccacg ctcgatgccg ccgcctcgct gccgccgccg
     9481 caaagctgca tactcgccgc tgcggacgcg cgtatgggcg aagtgtttta tgcgtggttc
     9541 gacacgctga accgccgccg tttgagcgat tatcaagtcg gtagggcggc agacattgcc
     9601 ctccccgagg ggtacgtctt ttcagacggc atcggcagtg cgttcgcact ggaaaaccgt
     9661 ccgcccttct caggcaaacc cgatatgccg accgccgccg attttctcgc gctcgccctc
     9721 ggcggctatc ccgcaactgg cgcggcacac gccgaactgc tctacgtccg caacaaaatc
     9781 gccctgactg ccaaagaaca ggcggaacgg aaggcacgaa catgaacatc cgcccagcct
     9841 taccgtccga ctgcgccgca ctggccgcgc tcgataccgt ctgcaacccg tccgcgtgga
     9901 cgcaacgcca atttgagtcc gcgcttgttt cgccgtccga acaggttttc cttgcggaaa
     9961 aagacggcag gattgccgcc tttatcgttt ggcagaacct gcccgacgaa tccgaactgc
    10021 acctgattgc caccgcgccc gaatgccgcc gacggggcgt tgcgtccgcc ctgctcgaat
    10081 attggtacgc acatctgccc gaaggcacgc aacgcctgct gctcgaagtc cgcgcaggca
    10141 acgccgccgc acaggcactg tataccaaac acggcttcag cattgcgggc aggcggaaaa
    10201 actattactg cgccgccgac gggcaaaccg aagatgccgt cttaatggag aaaatatgtt
    10261 aagcgcgcgc tacctccacc tgcacgaggc tttgggcttg ggtccgatgt ggctgaaaca
    10321 ggccgccgcc gtcctgccgc ccaaaaacac acccgcaacc ccggcacagg cacgtcccca
    10381 aaccgtccgc gccgccacga tccgcccttc ccaaccccat aacgttcagg cgcggctcga
    10441 aacgatgaaa gcgttggaaa ccgccgccgt acatacgcgc aaacccgcgc ctgaaaccga
    10501 aacgcccccg cccggccttt cagacggcat cgcccccgtt cccgccgctt cgggcattac
    10561 caagcttgcc gtcgtcagcc tgtgtccgcc gatcgaggat gcggtttacg ggcaactgtt
    10621 ccacggcaaa gcgggcatcc tgctcgacaa catcctcaaa gccgcaggac tggatgccgc
    10681 ctatgtccac aaaacctgtt gggtgaaaac cgccgccgtc ggtaacccga tgccgtctga
    10741 acaggccgtc gcggatgcgc tgggtcaaat cgccggggaa ctcgacggct gccgcgcccc
    10801 cgccgtccta ttcctcggac aggcgtttgt caacctggaa cggcaggcga tgattgaaac
    10861 tttgtgcggc agccgtccct tcttcatcat cgaccatccc gcccggctgt tgcgccaacc
    10921 cgaactcaaa gcccgaaact ggcaggtgtt gaaacagttg aaacgcgcct tgcggcaagg
    10981 cggcggcagt tgaagcgcgc cgcacggggc ggtagaatcg caactgcgtc ccaatatctg
    11041 acagaaagca caaaatgact gatttccgcc aagatttcct caaattctcc ctcgcccaaa
    11101 atgttttgaa attcggcgaa tttaccacca aagccggacg gcggtcgccc tatttcttca
    11161 atgccggcct cttcaacgac ggcgcgtcca cgctgcaact ggcaaaattc tatgcacaat
    11221 ccatcattga aagcggcatc cgattcgata tgctgttcgg ccccgcctac aaaggcatta
    11281 ttttggcggc ggcaaccgcg atgatgctgg cggaaaaagg cgtgaacgtc ccgtttgcct
    11341 acaaccgcaa agaagccaaa gaccgcggcg aaggcggcgt gttggtcggc gcgccgctta
    11401 aagggcgcgt gctgattatc gacgacgtga tttccgccgg cacatccgta cgcgaatcaa
    11461 tcaaactgat tgaagcggag ggtgcaaccc ccgccggtgt cgccatcgcg ctcgaccgca
    11521 tggaaaaagg cacgggtgaa ttgtccgccg ttcaggaagt ggaaaaacaa tacggcctgc
    11581 ccgtcgcccc catcgccagc ctgaacgatt tgtttatcct gttgcaaaac aaccccgaat
    11641 tcggacagtt cctcgaaccc gtccgcacct accgccggca gtacggcgta gaataaaaat
    11701 aaagcatatg ccgtccgaac cacctgccgc ttcagacggc atcaaaccga cacacaccga
    11761 gaaaacatca tgcccgcctg tttctgtccg cactgcaaaa cccgcctctg ggtcaaagaa
    11821 acccagctca acgtcgccca aggcttcgtc gtctgccaaa aatgcgaagg gctgtttaaa
    11881 gccaaagacc atctggcaag cacgaaagaa cctatattca acgatttgcc cgaagctgtt
    11941 tcggatgtca aactcgtcca ccgcatcggc acgcacgcca ttagcaagaa acagatttcc
    12001 cgcgacgaaa tcgccgatat cctcaacggc ggtacaaccc tgcacgatac gccgcccgca
    12061 accgccgctg ccgcacctgc cgccgcaccg caggtttccg taccgcccgc ccgtcaggaa
    12121 gggctcaact ggactattgc aaccctgttc gcacttatcg tcctcattat gcagctttcc
    12181 tacctcttca tcctatgaac gcgcccgaca gctttgtcgc ccacttccgc gaagccgccc
    12241 cctacatccg ccaaatgcgc ggcacgacac tggtcgccgg catagacggc cgcctgctcg
    12301 aaggcggcac cttaaataag ctcgccgccg acatcgggct gttgtcgcaa ctgggcatcc
    12361 gactcgtcct catccacggc gcgtaccact tcctcgaccg cctcgccgcc gcgcaaggcc
    12421 gcacgccgca ttattgccgg ggtttgcgcg ttaccgacga aacctcgctc ggacaggcgc
    12481 agcagtttgc cggcaccgtc cgcagccgtt ttgaagccgc attgtgcggc agcgtttcag
    12541 gattcgcgcg cgcgccttcc gtcccgctcg tatcgggcaa cttcctgacc gcccgtccga
    12601 taggcgtgat tgacggaacc gatatggaat acgcgggcgt tatccgcaaa accgacaccg
    12661 ccgccctccg tttccaactc gacgcgggca atatcgtctg gatgccgccg ctcgggcatt
    12721 cctacggcgg caaaaccttc aatctcgata tggtgcaggc cgccgcttcc gtcgccgtct
    12781 cgcttcaagc cgaaaaactc gtttacctga ccctttcaga cggcatttcc cgccccgacg
    12841 gcacgctcgc cgaaaccctc tcggcacagg aagcgcaatc gctggcggaa cacgccgcca
    12901 gcgaaacccg acgactgatt tcgtccgccg ttgccgcgct cgaaggcggc gtgcatcgcg
    12961 tccaaatcct caacggggcc gccgacggca gcctgctgca agaactcttc acccgcaacg
    13021 gcatcggcac gtccattgcc aaagaagcct tcgtctccat ccggcaggcg cacagcggcg
    13081 acatcccgca catcgccgcc ctcatccgcc cgctggaaga acagggcatc ctattgcacc
    13141 gcagccgcga atacctcgaa aaccacattt ccgaattttc catcctcgaa cacgacggca
    13201 acctgtacgg ctgtgccgca ctcaaaacct ttgccgaagc cgattgcggc gaaatcgcct
    13261 gccttgccgt ctcgccgcag gcacaggacg gcggctacgg cgaacgcctg cttgcccaca
    13321 ttatcgataa ggcgcgcggc ataggcataa gcaggctgtt cgcactgtcc acaaataccg
    13381 gcgaatggtt tgccgaacgc ggctttcaga cggcatcgga agacgagctg cccgaaacgc
    13441 ggcgcaaaga ctaccgcagc aacggacgaa acccgcatat tctggtgcgt cgcctgcacc
    13501 gctgaccgca acggaaagcc gccgcagaaa tgccgcctga accccgtttc agacggcatt
    13561 tccccgatga caaagacctg cccgaccctc aaggaccccg atgaaatcct accccgaccc
    13621 ctaccgccat tttgaaaacc tcgattccgc cgaaacgcaa aacttcgctg ctgaagcgaa
    13681 tgccgaaacg cgcgcgcgtt ttttagaaaa cgacaaggcg cgcgcacttt cagacggcat
    13741 tttgaatcaa atgcaggaca cgcggcagat tccgttttgt caggaacacc gcgcgcggat
    13801 gtaccatttc catcagaatg cggaatatcc gaagggcgtg taccgcatgt gtacggcggc
    13861 gacctaccgt tccggctatc ccgagtggaa aatcctgttt tcggtggcgg atttcgatga
    13921 gttgctcggc gacgatgtgt atttgggcgg cgtgtcgcac ttggtggagc agcccaaccg
    13981 cgcgctgctg actttgaaca aatcgggcgg cgatacggcg tatacgctgg aagtggattt
    14041 ggaagcaggg gaattggtag agggcggttt tcactttccg gcaggcaaaa accatgtgtc
    14101 gtggcgcgat gaaaacagcg tgtgggtgtg tccggcttgg gacgaacgcc agttgaccga
    14161 atcgggctat ccgcgcgaag tgtggctggt ggaacgcggc aagagtttcg aggaaagcct
    14221 gccggcgtac caaatcgata aaggcgcgat gatggtaaac gcgtggcgtt acctcgatcc
    14281 gcagggttcg ccgattgatt tgattgaagc gtcggacggt ttttacacca agacgtattt
    14341 gcaggtgtcg tccgaaggcg gggcgaaacc gttgaacctg cctaatgatt gcgatgtggt
    14401 cggctatctg gcgggacatc ttttgctgac gctgcgcaag gactggcacc gcgcgaacca
    14461 aagctatccg agtggcgcgt tggtggcggt gaaactgaat cggggcgaac tcggggcggc
    14521 gcagcttttg tttgcgcccg atgaaacgca ggcattggaa agcgtggaaa cgaccaagcg
    14581 ttttgtggtg gcaagcctgc tggagaatgt acaaggccgt ctgaaagcgt ggcggtttgc
    14641 cgacagcaaa tggcaggaag ccgagttgcc gcacctgccc tcgggcgcgt tggaaatgac
    14701 cgaccaaccg tggggcggcg acgtggttta tcttgccgcc agcgatttca ccacgccgct
    14761 gacgctgttt gcgctggatt tgaacgtgat ggaactgacc gtcatgcgcc tccagccgca
    14821 gcagtttgtt tcagacggca tcgaagtgcg gcagttttgg gcggtgtcgt ccgacggcga
    14881 acgcattcct tatttccacg tcggcaaaaa cgccgcgccc gacacgccga ccttagtcta
    14941 tgcttacgga ggtttcggca ttcctgaatt gccgcattat ctgggcagcg tcggcaaata
    15001 ttggctggaa gagggcaatg cctttgtatt ggcaaacatc cgcggcggcg gagaattcgg
    15061 cccgcgctgg catcaggcgg cgcagggaat cagcaaacac aaaagcgttg atgatttgtt
    15121 ggcagtcgtg cgtgatttgt ccgaacgcgg catgagttcg cccaaacaca tcggcttgca
    15181 gggcggcagc aacggcggcc tgattaccgc cgccgccttc gtgcgcgaac cgcaaagcat
    15241 cggtgcgctg gtgtgcgaag taccgctgac cgatatgatc cgttatccgc tgctgtccgc
    15301 cggttcaagt tggacggacg aatacggcaa tccgcagaaa tacgaagcct gcaaacgccg
    15361 gctgggcgaa ttgtcgccgt atcacaatct ttcagacggc atcgattatc cgcccgcact
    15421 cattaccacc agcctcagcg acgaccgcgt ccatcccgcc cacgcgctca aattctacgc
    15481 caaactgcgc gaaacctcgc cgcaatcttg gctctactcg cctgacggcg gcggccatac
    15541 cggcaacggc acccaacgcg aatccgccga caaactcgcc tgcgtgttgc tgtttttgaa
    15601 agaatttttg ggataagggc agggggcgac actgccgccg cgaatgaaaa aagatcgtct
    15661 gaaattgctt tttcagacga tcttttttaa tggttgtttc aaatcaaaat atctatgccg
    15721 tcggcccatc agcacttttt cacatccgaa ggcaaaaatc cgtaatgccg cctgaacgct
    15781 tcgttgaacc gtcccgcgtg gcggtagccg caaaagtgca tcgcggcttg gatggtgctg
    15841 cccgattcga tgagggcgag cgcgtgttcc agccgcaggc ggcgcaggcg tccggcgacg
    15901 gtttcgccgg tttgcgcttt gaaatagcgt ttcaggtagc attcgttcag cccgacgcgg
    15961 cgggcgattt cggcgatggt cagcgggcgg gcgaattcgc tgttcaaaat atcggcggct
    16021 tcgtctatgc gccggcggcg gtagccgttg tcgtggcggc ggaaggtgaa gcgtaataat
    16081 cgggcggaga gttccagcgc gtcggcttcg tcggcgagca ggccgaagcc gtccgattcg
    16141 aacgggcgtt gcagcagggg gcaggccgcc gccgtcagtg tccccgcgtt ttgcgccagc
    16201 cgttgcaggg cgaatcggcc tatcgtttgc ggcgaaaaca ggcgttcgtc cagtaaacct
    16261 tcgtcgtgcc agcggcgcag tttttccagc gaaaaatcca aatgcagcgc gcacatgccg
    16321 ccgctgtctg gcagcagggt ttcggatacg tccgccagtt cgccgcgtac cagccagatt
    16381 tcgccggcag acgggcggta ttccctgccg cccatttgta accggttctg ccccgatacc
    16441 atgacgaaca aggcgcagtt gtggctgaaa ttgtggattt cggtgggaaa cgcacccgtt
    16501 ccgccgccgc gcatccgtga taaggtaatg cccgagtcga agcggttgat gcacattttc
    16561 agatgcaaac cgggctgttt tgcctgcgca atgagtgcgc tgtcggaaca gccgtccaac
    16621 gcccagccgg atttatcgga gcggacatag gtttggtact ggcggtagat ggcggcggtg
    16681 ttcatgattg gataggaacg agttgtctaa caaatgaatt aaataggaat tattaccaat
    16741 aatcaagcgc agggattggt tggaacggaa aaggtcgtct gaaagggtgt ttcagacgac
    16801 cttttccgtt tcgggaattt gttttgccgt atcgggaatt ttgcgttttg cggcgtggtt
    16861 tctgcaggtt gtttgcttaa taataaacat tcttattcgt atgcaaagga accgcacacc
    16921 gtgaaaccgc gtttttattg ggcagcctgc gccgtcctgc cggccgcctg ttcgcccgaa
    16981 cctgccgccg aaaaaactgt atccgccgca tcccaagccg catccacacc tgtcgccacg
    17041 ctgaccgtgc cgaccgcgcg gggcgatgcc gttgtgccga agaatcccga acgcgtcgcc
    17101 gtgtacgact gggcggcgtt ggatacgctg accgagccgg gcgtgaatgt gggcgcaacc
    17161 accgcgccgg tgcgcgtgga ctatttgcag cctgcatttg acaaggcggc aacggtgggg
    17221 acgctgtttg agcccgattg cgaatccctg caccgccaca atccgcagtt tgtcattacc
    17281 ggcgggccgg gtgcggaagc gtatgaacag ttggcgaaaa acgcgaccac catagatttg
    17341 acggtggaca acggcaatat ccgcaccagc ggcgagaagc agatggagac cctgtcgcgg
    17401 attttcggta aggaagcgcg cgtggcggaa ttgaatgcgc agattgacgc gctgttcgcc
    17461 caaaagcgcg aagccgccaa aggcaaagga cgcgggctgg tgctgtcggt tacaggcaac
    17521 aaggtgtccg ccttcggcac gcaatcgcgg ttggcaagtt ggatacacgg cgacatcggc
    17581 ctgccgcccg tggacgaatc tttacgcaac gaagggcacg ggcagcccgt ttccttcgaa
    17641 tacatcaaag agaaaaaccc cggctggatt ttcatcatcg accgcaccgc cgccatcggg
    17701 caggaagggc cggctgccgt ggaagtgttg gataacgcgc tggtatgcgg cacgaacgct
    17761 tggaagcgca agcaaatcat cgtcatgcct gccgcgaact acattgtcgc gggcggcgcg
    17821 cggcagttga tacaggcggc ggaacagttg aaggcggcgt ttgaaaaggc agaacccgtt
    17881 gcggcgcagt agttttattc aaaacaaacc acaaggagaa ccccatgcaa aacgaaacca
    17941 tcaacctgaa acagcacctt gccgccatca aagaatactg gcagcccgaa atcatcaacc
    18001 gccacgggtt ccaattccgc ctggtcaaac ttttgggcga ttacggctgg cacacgcacg
    18061 gatacagcga caaagtgctg tttgccgtgg agggcgacat ggcggtggac ttcgccgacg
    18121 gcggcaacat gacgatacgc gagggcgaga tggcggtcgt gccgaagtcg gtgtcgcacc
    18181 gcccgcgttc ggaaaacggc tgctcggtgg tgctgattga tctgcccgac ccgtccgagg
    18241 ccgcctgaaa acgaagtttc cgaaggaagc tgagtttctg cgaagctaaa agcagcctac
    18301 accttcaata tgccaaaagc atagaggatt aacaaaaatc aggacaaggc ggcgggccgc
    18361 aggcagtacg aatggtacgg aaccggttcg cccggtgctt gggcgcctta gggaatcgtt
    18421 ccctttgagc cggggcggga caacgccgta ccggtttaaa gttaatccgc tatacaaccc
    18481 caccgcacac caacacacaa aggaaacccc atgacacgct tcaaatactc cctgcttttt
    18541 gccgccctgc tacccgtgta cgcgcaggcc gatgtttctg tttcagacga ccccaaaccg
    18601 caggaaagca ccgaattgcc gaccatcacc gttaccgccg accgcaccgc gagttccaac
    18661 gacggctaca ccgtttccgg cacgcacacc ccgttcgggc tgcccatgac cctgcgcgaa
    18721 atcccgcaga gcgtcagcgt catcacatcg caacaaatgc gcgaccaaaa catcaaaacg
    18781 ctcgaccgcg ccctgttgca ggcgaccggc accagccgcc agatttacgg ctccgaccgc
    18841 gcgggctaca actacctgtt cgcgcgcggc agccgcatcg ccaactacca aatcaacggc
    18901 atccccgttg ccgacgcgct ggccgatacg ggcaatgcca acaccgccgc ctatgagcgc
    18961 gtagaagtcg tgcgcggcgt ggcggggctg ccggacggca cgggcgagcc ttctgccacc
    19021 gtcaatctgg tacgcaaaca cccgacccgc aagccattgt ttgaagtccg cgccgaagcc
    19081 ggcaaccgca aacatttcgg gctgggcgcg gacgtatcgg gcagcctgaa cgccgaaggc
    19141 acgctgcgcg gccgcctggt ttccaccttc ggacgcggcg actcgtggcg gcagctcgaa
    19201 cgcagccgcg atgccgaact ctacggcatt ttggaatacg acatcgcacc gcaaacccgc
    19261 gtccacgcag gcatggacta ccagcaggcg aaagaaaccg cagacgcgcc gctcagctac
    19321 gccgtgtacg acagccaagg ttatgccacc gccttcggcc caaaagacaa ccccgccaca
    19381 aattggtcga acagccgcaa ccgtgcgctc aacctgttcg ccggcataga acaccgcttc
    19441 aaccaagact ggaaactcaa agccgaatac gactacaccc gtagccgctt ccgccagccc
    19501 tacggtgtgg caggcgtact ttccatcgac cacagcactg ccgccaccga cctgattccc
    19561 ggttattggc acgccgatcc gcgcacccac agcgccagca tgtcattgac cggcaaatac
    19621 cgcctgttcg gccgcgagca cgatttaatc gcgggtatca acggctacaa atacgccagc
    19681 aacaaatacg gcgaacgcag catcattccc aacgccattc ccaacgccta cgaattttcc
    19741 cgcacgggcg cctatccgca gccatcatcg tttgcccaaa ccatcccgca atacgacacc
    19801 aggcggcaaa tcggcggcta tctcgccacc cgtttccgcg ccgccgacaa cctttcgctg
    19861 atactcggcg gcagatacag ccgctaccgc gcaggcagct acaacagccg cacacaaggc
    19921 atgacctatg tgtccgccaa ccgtttcacc ccctacacag gcatcgtgtt cgatctgacc
    19981 ggcaacctgt cgctttacgg ctcgtacagc agcctgttcg tcccgcaatt gcaaaaagac
    20041 gaacacggca gctacctgaa acccgtaacc ggcaacaatc tggaagccga catcaaaggc
    20101 gaatggcttg aagggcgtct gaacgcatcc gccgccgtgt accgcgcccg taaaaacaac
    20161 ctcgccaccg cagcaggacg cgaccagagc ggcaacacct actatcgcgc cgccaaccaa
    20221 gccaaaaccc acggctggga aatcgaagtc ggcggccgca tcacgcccga atggcagata
    20281 caggcaggct acagccaaag caaaccccgc gaccaagacg gcagccgcct gaaccccgac
    20341 agcgtacccg aacgcagctt caaactcttc accgcctacc acttagcccc cgaagccccc
    20401 agcggccgga ccatcggtgc gggtgtgcgc cggcagggcg aaacccacac cgacccagcc
    20461 gcgctccaca tccccaaccc cgccgccaaa gcccgcgccg tcgccaacag ccgccagaaa
    20521 gcctacgccg tcgccgacat catggcgcgt taccgcttca atccgcgcac cgaactgtcg
    20581 ctgaacgtgg acaacctgtt caacaaacac taccgcaccc agcccgaccg ccacagccac
    20641 ggcgcactgc ggacagtgaa cgcggcgttt acctatcggt ttaaataagg tcgtctgaaa
    20701 acggagtttc tgcgaagcta aaagcagcct gcacattgaa aatgccgccc aagcaaacct
    20761 gcagtttgcc gccctctccc tagccttctc ccacggggcg agggaattgg ggtgcaggct
    20821 gcctttaagg ttcaggcaaa ttttaacttc gttgatactg cgctttagct tcgcagaagc
    20881 tgcactttca gacgaccttt atggaacacc agaggtacac gcatttaagg aatgccgtct
    20941 gaagccgcct tcaataccgc gccatttccg cgtcaacctc aaccgcccac gcgtcgatgc
    21001 cgccctgaag gttgtacagg ttttcaaaac ccgcctccgc caaatacatc gccgtatgca
    21061 ggctgcggat accgtgatgg caatacacca caagcggcac atcgtccggc aactcgtttt
    21121 gccgcagcgg aatcagattc atcgggatat gcagcgcatt tggcagcgaa caaaccgccg
    21181 cttcttcatc cgtacgcacg tccaacaaac aaaacatccg cccttcgtcc atccacgcct
    21241 tcaatgccgc cgacggaagt tgcacaatat ccatcacaaa ccccccgaaa aaaaccaagc
    21301 aaaatgccgt ctgaagcccc aaacccgctt tcagacggca tcacaatccg aacatcaaaa
    21361 attaaaactg tcgaaaggat gggcatcctt gtcgtccaaa tgcgccacca aagtatcgaa
    21421 cagcaccttc tcttcaaaca catcgcccct gcgcgtaatc aaaagcgcgc gctgcaccgg
    21481 cctgcgtccc acaataaccg ccatacgccc gccgtccttc aactgttcct ttaacacttc
    21541 aggcacaagg gttaccgcgc cgccgacgta aaccgcatca aacggcgcac ctgcggaaag
    21601 ttcggtcaac ccgttatttt gcacataatc gatgttttcc aaagacaagc cgtccaaaac
    21661 cgctttggcg cgcttttgcc gttcggcatc gatgtcgtcc gaaaccacac gaccagccaa
    21721 ttttgccaaa agcgcggtcg catagcccga acccgtgccg atttccaaaa ccgtatcgtt
    21781 tttcgtcagc ttcaagccct gcgccagccg cgccacgact ttcggctcca gcatcttatg
    21841 accgttggca agcggcagcg ccatatccgc atacgccaaa ccctgcaaat cctcatcgac
    21901 aaaaagctcg cgcggaatct ccgccaaagc gtccaaaacg tcaaaatcca atacatccca
    21961 cggacggatt tgctgttcga ccatattgaa ccgcgctttt tcaaaatcca tttagtgctc
    22021 cgtcaaataa ttgtttcaac aatggcaggc attataaaac cacactcccg ccgcgccaac
    22081 ttcggcacgc gccgcccgcc cctttccatc actcaagccg cctggcgcga agcctcaaac
    22141 cgcttctcca aaatcttcgc cagttcgccc aaatacaaag catctgtctc atcaaactgc
    22201 gccaaatgtt cgctgtccgc gtccaacacg ccgatacagc ggccgtctga aaacagcggc
    22261 acgacaatct ccgaacgcga caaagacgaa caggcaatat ggtcgggatg cgcgtccaca
    22321 tccccgacga ccaccgtccc gcccttcgcc caagcctgac cgcacacccc gcgaccgaac
    22381 ggaatccgcg tacacgccaa aggcccctga aacggtgcca aaaccaattc gtccgaacgc
    22441 gtatcgacca aataaaaacc cacccaaaac caaccgaacg cctccttcaa aaccgccgcc
    22501 gtgttcgcca aattcgccac ccaatccgtc tcgtccgcca ccacagactc aatctgcggc
    22561 aacacctccc gataaagcgc ggccttgtcc gaagccgaaa aatgaagcgc gtgcatcaca
    22621 tctcctatag ttgcatacat atcaggcggc cattataaaa caacctgccc gaaacaacat
    22681 tccaaaccgc ccccgcagcc gcccgcccca agttgcgaac ccgccgcata tccactaaac
    22741 ttcacattgc aacgcgccac acgcggcaga cagaaaaaac acgacacgga gcaaaaaaga
    22801 tgtatcacca aatcggaatg tgggatcaaa aatgggtcat cggcaactgg aaaatgaacg
    22861 gccggctcca gaacaacaat tcgctcatgc accgcttccg catcctcccc accgccgaac
    22921 gcgtcctcat cggactcgcc gccccgaccg tttacctgct gcaactgcac aacgccatgc
    22981 aaatcgtctt aaacaaccgc atcctcacct gcgcccaaga cgtgagccgc ttccccgaca
    23041 acggcgcgta caccggcgaa gtgtccgccg aaatgctcgc cgacaccggc acagacatcg
    23101 tcctcatcgg acactccgaa cgcagccttt atttcggcga aaaaaacgaa atccaacgcc
    23161 gcaaaatgga aaacgtcctc aacgtcggac tcatcccgtt attgtgcgtc ggcgaaagcc
    23221 tcgaagagcg cgaagccggc aaagaacacg aagtcatcgc ccatcagctt tccatcctgc
    23281 aagggctgga taccaaaaac atcgccgtcg cctacgaacc cgtctgggcg atcggcaccg
    23341 gcaaagtcgc caccgtcgaa cagattgccg atatgcacgc attcatctac aaagaaatct
    23401 tgtctttgtg cggaagcgat gttaaaatcc gcgtccttta cggcggaagt gtgaaagcgg
    23461 acaacgcggc cgacatcttc gcagtacctt atgtggacgg cgcactcgtc ggcggcgcgt
    23521 cattgtcgta cgactccttt accgccatca tcagtgccgc acaaaatgcg tagaaaaata
    23581 tggaagcctt caaaacccta atttggattg ttaatataat ttccgctttg gccgtcatcg
    23641 tgttagtatt gctccaacac ggcaaaggcg cggatgccgg cgcgactttc ggatcgggaa
    23701 gcggcagcgc gcaaggcgta ttcggctctg ccggcaacgc taacttcctc agccgctcga
    23761 ccgccgttgc agcaacattt ttctttgcaa cctgcatggc tatggtgtat attcacaccc
    23821 acacgacaaa acacggtttg gacttcagca acgtacaaca aactcagcaa gcacccaaac
    23881 ccgtaagcaa taccgaacct tctgcccctg ttcctcagca gcagaaataa cagtttttca
    23941 aatgccgaca tggtgaaatt ggtagacacg ctatcttgag ggggtagtgg ccgtaggctg
    24001 tgcgagttca aatctcgctg tcggcaccaa cacacaaaac gcttagaaat tctaagcgtt
    24061 ttttttcgtt taatcctgtc taatgccgtc aaccaatccc aatcaataca aggatttcag
    24121 gctgattgtt atcctgccgt cccccttcct gacagtgcaa tcccgtccaa tccgccctaa
    24181 ttgaactaac ctgatttttt acagcatccg aaaataccgc gagaaaatac cgtaaaaata
    24241 aagctgaacg accaccaaat caggaatgcc aagcgggaaa tagcttgcgg ggaatactga
    24301 cgcaccgtag ggaatgaggg ggaaaccgtc caagatgcag agcggttttt tttgggtttt
    24361 tggaaaaaac ctatactata gtggattaaa tttaaaccgg tacggcgttg cctcgccttg
    24421 ccgtaccggt ttaaatttaa ttcactataa aataagaaaa agataaaaaa ttggtaacaa
    24481 atgcggtaac aaatggtaac gaatcggtaa caacttttgg ggttttccgg tttttcaccg
    24541 tcttggcagt gggagcgtag cggaatgaaa agccaaaacg cacggaaccg cgcctatttt
    24601 gagcaggaat ggcggttaaa ccgcttggtt atatacgggg cataggaaga cagcgaaacg
    24661 cgctaaaaac gcaatttgag ccattaaaag cgattgatta aaaaataatc aggttagccg
    24721 ccggtgtttc aggcggcata aacggagaaa ttgcggggca taaaaaaggc agcttgccgt
    24781 gttgtctgtc tctggtataa ttccaagtat cactaatcaa cggctacaca atgcggatat
    24841 tcaaaaacca atggatagtg aaatttgcca agaagcacaa aatcaacgat tccgagctgc
    24901 tggaagcggt agagcgggcg gataacgggc tgatagacgc agatttaggc ggcggtgtga
    24961 ttaagcagcg catagcaagg caaggacaag gcagaagcgg cggctatcgc agcctgatat
    25021 tgttcaaaca ggcagatagg gcatttttcg tttacgcctt tgccaagaac gacagggaaa
    25081 acatttcgga taaagaactg gacgtttacc gaaaagccgc cgcatattat ctgaaataca
    25141 cgcgggcaga gctggcggct ttgaaagaag acggcattat cacggagata gaattatgaa
    25201 atacaaaagc gaggcattag ccgccattca tgaaatgatg gaaggggctt acaacatcgg
    25261 cgcaatcgac aaaaagacta tgcgcggctt tgacaagtcc tgcctgaccg aaatcaaacc
    25321 gttgagcggc ggggacatca aggcaatcag ggagaaggag gcactatcgc aagccgcttt
    25381 cgccatctat ctcaacgtgg gaaaaaatca cgtttcggct tgggagcggg gcgttaaaaa
    25441 gccgagcggc gcggcgttga agctgctgac catcgtcaaa aacaagggta tcgaagccat
    25501 tgcgtagccg acttggcaaa cggcaaaatc agcaagttca caatagacgc gctgctgaat
    25561 atgcctgcca agacaggcaa gaccgccgaa ccgaatatca gggcatagcc gcacaaatgc
    25621 ccgaccgcat caaaccaagc cgaaacggcg gcggtgcaga cggcatagcc cgacagcaag
    25681 gcacggcgca gacgggcgcg aaacccgaaa catccccgac cgcgaggcac ggggattttt
    25741 tgcgcccgtt gcagggggat tagatttaag cggcgcgggc ttgaaggcaa aacgggcggg
    25801 gcgcagaact gtttaaatgc cgtctgaatc tcaaacgatt tcagacggca ttttgaaaca
    25861 acggctcaaa ttctcgatcc ccttccctta acgccgacgt tttttattaa cgcgcccctt
    25921 atttctgaca ctttgctcat aaaccggcat aacggtcggc aacaaccgtt ttagattttc
    25981 tatacaggca ttgtttacag catcagcata agacaccgta ctctggaata ccgcctaaac
    26041 cctgcggata gttttggatg tggtatcgac attgtcggca gacttgttta actcgaccgt
    26101 tttcaaataa tctttgacag atgctgcatt cagtgtcgcg gaatccgcgt attttgaaca
    26161 aaaggtcggg ctactttatc acaatagcag aaagatggaa aacggcgggg attggtgaaa
    26221 ggtcgtctga aatgatattc agacgacctt gaacggttgt gttttaaata tggtatttat
    26281 tcatttgttt caccatcagg cagtccaaac caccttaagg gaatcgtgtt aaattggtaa
    26341 tggcagttaa cactgataat ataatctaaa acatctttaa tattccggtt ttgtaaacca
    26401 atcattcaaa acatagacat atccaacccg ccaacctaaa gagtgaacta actttgtata
    26461 ttgttttctc ttaaaatcgc aagtttgtaa tttctcatct actgaaccgg gagtttgttg
    26521 gaatttgatc tcaataataa ataaggtatc acggatgatg ataaataagc cattatcggg
    26581 cagtaatctt ttagacagat ggtctttcca attaatattg tattccagtc tttccaaaaa
    26641 tcggtaaaac tcatattttt tgaaacaata agccttcaat tctccattaa accaaacttc
    26701 ataacccgca ttatcatctg tttttcttaa gtcgtacccg ggaatttctt caaacaactt
    26761 tcgtaagtct gtacgttctt cgaatctgag tcctgtttgt gtccttgcac cgccgattcc
    26821 gccaataatc atttttctaa ccttttcttt gctaattcaa aaaaatcttc ttccagttcg
    26881 caaccgcaaa atctccgacc atgttttaag gcggcaacgc ctgttgtgcc gctgcccata
    26941 aaagggtcaa aaattaaatc tccgatattt gaagccgata gtatgcagcg ttcaagtaag
    27001 gggagtggtt tttgtgtcgg atgtttgccg aatgtttttt cggttttatt tggtggagga
    27061 aatgtccaaa cacatttcat ctgtttgcca tcattttgtg ttttcatggt ttcataatga
    27121 aacgtgtgtt tagctttttt atttttcttt gcctataaga ttgtttctgt cgaatgggta
    27181 aagaaacggc aggacaaatt agggggagga ttgggttttt cccaagtaat attgttcaaa
    27241 atatggtagc cgacggtttg catcaggtag ccgattaaat agatattatg aaatgtgccg
    27301 caaacccaaa ttgtgccgtt tggttttaat aaggcgtaac acagtcggag ccattcttcg
    27361 taaaattcca aatctgccgc cattcctttg gatttatccc agttgccctt attgacggaa
    27421 accatttgcc cgttttgaca actgaaaccg tcattggaaa gaaagtaagg cgggtctgcg
    27481 aaaatcatat caaaacagcc gtttggatgt ttttccaata tcttccgcat gacgttaagg
    27541 gaattttcgt tgtagaggac aaggtcgtct gaaacgattt tttcaaacga ctttgataaa
    27601 tggcagcttc ttcagccggt ttgtcaaaat caatcataaa ttgccaataa ttctccgact
    27661 tttttacgac tgctgccctt tgcggcaata ttgcgttgta cttcaaccgt ttttaaaatg
    27721 gcagaagaat aaatatttct cgtaaattca gtatcatgat ttgaaatcaa aacgccttgg
    27781 gagttgggtg caatttgtcg atttttttgt aaagtccgcg accaatgaat tcgatcatat
    27841 tttgatcacg caaaatttgc aactgttggc ggattttgtc tttgatatgg ttgttttggg
    27901 gaaattggat ggatagtttg ttttcaaatt catacatttg cgacaatgtg aattcttcgg
    27961 tgagttggtc gatacatttc ataacagcca aaagccagtc tttgcgctcc gcattttggt
    28021 tgcgtaaaaa caggttggat tgccattttt tcagaacggt ttcgggttcg ataatgcggg
    28081 aatcgtctat taagaatatt tttccgcttt caggcaaagg ggcgagattg atggaacaca
    28141 taatgtggtg cggtcggttt ttaatgcctt tatttctggg aataatcata tccggcgtga
    28201 tgaaatgttt gggtacaagc accaattgcc gtatggagta atccgctttt ttatatgcaa
    28261 gaaagaaaaa gttggggttg gtatctgacc ggatgcgctc caacatagtg cgatatgcac
    28321 cgtcaggcac gctgttgcct atggtttttt gatttttact ctttaattca tattgctcgt
    28381 ggcaatttgg gcaaaagagg tctgcaacag gtttgttatt ggcaaatctc tgcatcggct
    28441 tgcttccgca acaggggcaa tagccgtttt tttccagcca agcctcgctc attacacgga
    28501 ttttgtgggt tgctttattt tgttgctttc ccaattgggt atcgaaaaat aaattcatat
    28561 tttggatttt gagattaatt tttagtagat taacaaaaat caggacaagg agacgaagcc
    28621 gcagacagca gggcaaggcg gggcaacgcc gtaccggttt aaagttaatc cgctatattt
    28681 gaggtctgtc ttgcagacaa cccaatctgc cttaaaaagg ccgtctgaaa ccctgtttcc
    28741 aagtttcaga cggcctttat ccgtatggct aaaccttaaa agcggtttag acgacgatgt
    28801 tcaccagtct gcccggtacg acgatgattt tcttcgcagg cttgccttcc atgaatttca
    28861 ctgcgccttc gttggcgagt gcggcggctt cgaggtcggc tttggaggcg tcggcggcga
    28921 cggtgatttt gccgcgcagt ttgccgttga cttgaaccat cacttcgatt tcggatttga
    28981 ccagggcggc ttcgtcgact gtcggccagc ctgcttccca cagtttcgcg ccgttcaatt
    29041 cgctccacag ggtttcgcag atgtgcggca cgatgggcca caacaggcgt acggcggctt
    29101 ccaatacttc ttgggcgacg gcgcggcctt gttcgctgcc ggtgtcggtt ttgtcgtatt
    29161 ggttgagcag ttccatcacg gcggcgatgg cggtgttgaa ctgctggcgg cggccgtagt
    29221 cgtcgctgac tttggcggtg gtggaatgca gtttgtggcg caggtctttg agttctttag
    29281 acaaaccgtc ttggttgcct gcaaatgctt tgaccgcgcc gccttgcttc aggtattcgt
    29341 aaacggtacg ccacagacgg cgcaggaagc ggtgtgcacc ttcgacgccg ctgtcgctcc
    29401 attcgaggga ctgttcgggc ggtgcggcga acatcatgaa caggcgggcg gtgtccgcgc
    29461 cgtaggcgtt aatcagttct tgcggatcga cgccgttgtt tttggatttg gacatttttt
    29521 ccgtgccgct gatgacgacg ggcagtccgt cggctttgag gacggcggaa acggggcggc
    29581 ctttgtcatc gaaagtcagc tcgacatcgg cggggttgat ccagtctttg ccgcctttgt
    29641 cgttttcgcg gtagtaggtt tcgcagacga ccataccttg cgtgagcagg cgttcaaacg
    29701 gttcgtcaac attgaccaaa ccttcgtcgc gcatcagttt ggtgaagaag cgcgcgtaca
    29761 ggaggtgcaa aatcgcgtgt tcgatgccgc cgatgtattg gtcgaccgcg ccccagtatt
    29821 tcgcggattc tgccgatacc atgccgtctg aaaacttggg cgacatatag cggaagaagt
    29881 accagctcga ctccatgaag gtgtccatgg tgtcggtttc gcgtttcgcc gcgccgccgc
    29941 agcacgggca ggaagtttcg taaaactcgg gcatttttgc cagcggcgaa cccataccgt
    30001 cgggtacgac gttttcaggc aggacgacgg gcagttggtc ggcagggacg ggaacgtctc
    30061 cgcatttttc gcaatggacg atgggaatcg ggcagcccca gtagcgttgg cgcgaaatgc
    30121 cccagtcgcg caggcggtat tgggttttcg gttcgcccgc accttggctt tgcagcttgg
    30181 cggcaacggc atcaaatgcc gtctgaaaat ccaagccgtc caagtcgccg ctgttgacca
    30241 atacgccgtt ttctttgtcg ccgtaccatt cttgccatcg gtttgcgtcg aatgcgttgt
    30301 cgccgacggc aatgacttgt tttttcggca gattgtattt ggcggcaaac tcgaaatcgc
    30361 gttcgtcgtg cgccggaaca gccatcaccg cgccgtcgcc gtagccccac aagacatagt
    30421 tggcaatcca cacttccagc ttgtcgccgt tgagcgggtt gacgacgtag cggccggtcg
    30481 gcacgccttt tttctccatc gtcgccatat cggcttcggc aaccgaaccg gctttgcatt
    30541 cggcgataaa tgcctgcaat tcgggtttgt cggcggctgc ggctgttgcc agcggatgtt
    30601 cggcggcaac agcgacataa gtcgcaccca tcagcgtgtc ggggcgggtg gtataaactt
    30661 gcaggaattt cgcgtaatcg ccttccaaac cttgcttgct gtcgtctgaa acggcgaagc
    30721 gcacggtcat gccgcgagat ttgccgatcc agttgcgctg catggttttg acttgttccg
    30781 gccagtgttc cagcttgtcc aaatcgttga gcagctcttc ggcgtaatcc gtgattttga
    30841 agtaatacat cgggatttcg cgtttttcga tcaacgcgcc cgaacgccag ccgcgtccgt
    30901 cgatgacttg ctcgttggca aggacggttt ggtcgaccgg gtcccagttc accgtgccgt
    30961 ttttgcgata gacgatgcct ttttcaaaca gcttggtaaa cagccattgt tcccagcggt
    31021 agtattcggg tttgcaggtg gcgacttcgc gttcccagtc aaccgcaaaa cccaggcttt
    31081 tgagctgggt tttcatgtat tcgatgttgt cgtaggtcca agcggcgggg gcgacgttgt
    31141 ttttcatcgc cgcattttcc gccggcatgc cgaacgcgtc ccaacccata ggctgcataa
    31201 cgttgaagcc gtttaaaagt ttgaagcggc tcaatacgtc gccgatggtg tagttgcgta
    31261 catgccccat atgcagcttg ccgctagggt aagggaacat cgaaaggcaa taatatttgg
    31321 gtttggaagc gtcttcggag acgttggaaa tacgggcgtc gtcccatttt ttctgcgccg
    31381 caggctcgat ggcggcgggc tggtaatgtt cttgcatggt cattttgttt ttcgcttgaa
    31441 aacgttagga atttaaagga ggaattataa caggttgccg atgtcggcga atcggcaaat
    31501 tgccggcagg atgcgtaaat tcgcacgcgc atcattccgt atgccgtaca aatacaccgc
    31561 gtttactgat acgcacgttt tttatgctaa tattacgaac caaaatcaaa cgtttaaaac
    31621 tctcctgatg cggctcttcc gaacaaaagg cagacgggca tcgggtaaaa aaggattctg
    31681 catatgaaaa tcaaacaaat cgtcaaaccg agcttggcag tattggcggc aggcattctg
    31741 tctgcctgcg caaccataaa gcaacgtcaa agccgacggc acgaccgaca atccggtttt
    31801 cccggaaccc tattccgtaa cgctcgacaa caagcgcggc acattcccga cttatgacga
    31861 actggatcag atgcgccccg gtctgaccaa agacgacatc tacaaaatcc cgggccgccc
    31921 gcattacgac gaaggtatgt acggcgtgcg cgaatgggat tacctgttcc acttccacac
    31981 cccgggcgtg ggtatcgacc ctgaaaacac ttccggcgtg gaaggcgtta ctacctgcca
    32041 atacaaagtg attttcgata aagacaaatt tgcccgcagc ttctactgga accccgtctt
    32101 cccgaaagat gccgtctgcc cgccgcccgc acccaaagcc gagccgcaag tcatcatccg
    32161 cgaaatcgtg ccggcaaaac cgaaacgtat ccgccaataa gccgctatgc cgccccgcct
    32221 gctttcaggg atattgtgct gcctgctgac gactgccccc gtattcgcac aggggcagcc
    32281 ggatgcggtc agtgcctata ttcaaaagaa aaaagtcatc gtcgacacat ccaaggcgga
    32341 actctgtttt gccgacgacc gtcagtgcca ccccgtcctc atcggcactg ccacacccaa
    32401 ggggacgttc ggactgacgc tgaacagtac cgacaagccc ggttacggcg gcgaagtcat
    32461 cggtttcaag caggagggcg attttctttt cgccctgcac cgcgtttgga atcagatacc
    32521 gtccgaaagg cgaaacgaac gcatcgcctc cccgtccgtg tccgacagga ttatgaccaa
    32581 cggctgcatc aacgtcagcg atgcggtgta cgaaaaactg cgtcattatt ttgtgttgga
    32641 agtgatttga aacagacgga taccgcacgt gccggtatcc gttttcacat tgccccgatg
    32701 cctgaaacag accgtccgtc acgtcatgcc gcctgaaacc ggcgcagatg ccgccaagcc
    32761 ttcagacggc attgtccgcc cgctccgacc gaacaacaac catctttggg agaaccttat
    32821 gaccgaacaa aaccgcatcc tctgccgcga actgagcctg ctggcattca accgccgcgt
    32881 gttggcacag gcggaagaca aaaacgtccc ccttttggaa cgcctgcgct tcctgtgcat
    32941 cgtgtcgtcc aatcttgacg agttttttga agtccgcatg gcgtggctca agcgtgaaaa
    33001 caagctgcac ccccggcgca ggccggacaa cggcaaaacg ccgtctgaaa ccatcgccga
    33061 cgttaccgaa gcggcgcgct ccctgatacg gcaccagtac gacctgttca acaacgtcct
    33121 tcagcccgag ttggcgcggg aaagcatcca tttttaccgc cgccgcaatt ggacgggcac
    33181 gcagaaaaaa tggatcgaag actatttcga ccgcgaactg ctgccgatcc tgacccccat
    33241 cggactcgac ccttcccacc ctttcccgcg cccgctgaac aaatcgctca acttcgccgt
    33301 agaactcgac ggcacggacg cgttcggcag gccttcgggt atggcgattg tccaagcacc
    33361 gcgcatcctg ccgcgcgttg ttcccctgcc gtccgaactg tgtggcggcg gacacggctt
    33421 cgtcttcctg tcgtccatcc tccacgccca cgtcggcaaa ctcttcccgg gcatgaacgt
    33481 caaaggctgc caccagttcc gcctcacgcg cgacagcgac ttgaccgttg acgaagaaga
    33541 cgtacaaaac ctccgcgccg ccattcaaaa cgaactgcac gaccgcgaat acggcgacgg
    33601 cgtgcggctc gaagtcgccg acacctgtcc cgcctacatc cgcgactttc tgctcgcgca
    33661 attcaggctg accgacgccg aactctatca ggtcaaaggc ccggtcaacc tcgtccgcct
    33721 caacgccgtc cccgacctag tcaaccgccc cgatttgaaa tttccgccgc acacgccggg
    33781 tcgtctgaaa gccttgggca aaaacagccc gatcttcgat ttggtgcgcc aatcgcccat
    33841 cctgctgcac cacccctacc aatctttcga tcccgtggtc gatatgatac gcgaagccgc
    33901 cgccgatccc gccgttcttg ccgtcaaaat gacgatttac cgcaccggca cgcgctccga
    33961 actcgtcccc gccctgatga aggcggcact cgccggcaaa caagtaaccg tcgtcgtcga
    34021 actgatggcg cgttttgacg aagccaacaa cgtcaactgg gcgaagcagc tcgaagaggc
    34081 gggcgcgcac gtcgtgtacg gcgtgttcgg ctacaaagtc cacgccaaaa tggcactggt
    34141 catccgccgc gaagacggcg tgctcaagcg ttacgcccac ctcggcacgg gcaactacca
    34201 ccaaggcaca tcgcgcatct acaccgactt cggcctcatt accgccgacg aacaaatcac
    34261 cgccgacgtg aacacattat ttatggaaat cacaggcttg ggcaaacccg ggcggctgaa
    34321 caaactctac caaagtccgt ttaccctgca caaaatggtt atcggccgca tcgctcgcga
    34381 aaccgaacac gcaaaagccg gcaaaccggc gcggattacc gccaagatga attcgctcat
    34441 cgaaccgact gtcatagaag ccctgtatcg ggcaagcgcg gcaggcgtgc aaatcgattt
    34501 gattgtgcgc ggtatgtgta ccttgcgccc gggtgtaaaa ggcttgtccg aaaacatccg
    34561 cgtccgctcc atcgtcggca ggcagctcga acacgcgcgc gtatattgtt tccacaacaa
    34621 cggcgcggac gacaccttta tttccagcgc ggattggatg gggcgcaatt tcttccgccg
    34681 catcgaaacc gccaccccga ttaccgcgcc cgaactcaaa aaacgcgtca tccgcgaagg
    34741 tttggaaatg gcactggcag acaataccca cgcgtggctg atgcagcccg acggcggcta
    34801 tatccgcgcc gcgcccgccg aaggcgaaag cgaagccgac ctgcaaaacg atttatggga
    34861 tttgctccga ggctgacccg cacaagcact tcccccatac gctcccaacc ccaatccgcc
    34921 gcattccaga cggcattgcc tttccggcag ccgaacaaaa ccccgaatcc gtctgcacgg
    34981 ttcggggcat atctccaata cgggcatcgt gttccggaaa accgtcaaat ccgcatcggc
    35041 atcacaatat atttgaaatt cggattgttc ggcacggtaa acagcgtcga gcggttggcg
    35101 tcgccgaagg caagctgcat atcgtcggaa tggatgttgc gcaacacgtc catcaaatag
    35161 ccgatattga aaccgacttc gagttcgccg ccctgatagg cgatttcgat ttcttcgcgc
    35221 gcttcttcct gctcgttgtt gctgcacacg acgctcaaca ggccgggttg caggaacagg
    35281 cgcgcgccac ggaatttttc gttggcaaga atggacacac gttccagcgc gcccaaaagt
    35341 tcggcacggg acaacacgaa aatcttgtcg ttgtccaaag gaatcacgcg gttgaaatcg
    35401 gggaatttgc cgtcgatgac cttgctgacg atggtcgtgc cgttgcattg gaaacgcacc
    35461 tgcttgtcca gcagctcgat ttgaatcgga tcgtcggggt tgttcaacag tttgaacagt
    35521 tccagcaccg ttttgcgcgg caaaatcact tcggcgcgcg gcaaatccgc atcaatcgcg
    35581 caggctgcat aggcaaggcg gtgtccgtcg gtcgccacaa ggcgcaactg gctgccctca
    35641 acctgcatca gcagaccgtt gagataatag cggatgtcct gcaccgccat gctgtactgc
    35701 acttgcgaca gcatggtttt gaaacgctcc tgccccagcg agaaagtcgc gctgatgtcc
    35761 tcgccgacat tcatcatcgg aaaatcggcg gcaggcaggg tttgcagggc aaaacgcgat
    35821 ttgcccgcct tcagcgtcag acggttgtcg tcccaatcca gcgacaccag cgcaccggca
    35881 ggcagcgcgc gcaaaatgtc ctgaaatttc ttggcattgg tagtgatgcg gaagtcgccc
    35941 gcacctccct cgggacccgc ggtgtcgatt tggatttcca aatcggttgc caagagtttg
    36001 gtctgaccgc ccctgccctc aatcagcaca ttggacagga tgggcagggt gtgtcggcgt
    36061 tcgacgatgc cggtaacggc ttgcagcggc ttgagcaggc tgtcgcgctc ggcttgtaaa
    36121 atcaacattt tcgctcctta aatcggttgg atttcctacc cttcggacgg catggtcaat
    36181 gtcatgccgt ctgaaaggcg tgtccggtca gttttgaatc agaatcagca gtttctcgta
    36241 atcctgcgcc aactcggggt cttcctcgcg cagtttcgcc accgccctga tgccgtgcat
    36301 gacggtcgta tggtcgcgtc cgccgaacga atcgccgata gacggcaggc tcaaagtggt
    36361 caattctttg gtcaggctca tagcaacctg acgcggacgg gcaatgttgc gcgtgcgttt
    36421 tttgccgagt acgtcgctga ttttgatgcg gtaatatttc gccaccgcat cgatgatgat
    36481 gtcggcggtg atgactttgt gcttctcggc aataatgtcc tgcaaagcgg tacgcgccaa
    36541 atcgatgtcg atgacgggac ggttcataaa gcggctgctc gctccgacac ggttgaacgc
    36601 gccttccagt tcgcgcacgt tggaacggat cagattggca atgaacagcg cggcttcgtc
    36661 ttcgatactg atgcccgccg cttccgcctt tttctgcaaa atggcgatac gcatttccaa
    36721 ttcgggcggc tcgagttcca aagtcagccc ccacgaaaag cgggatttga ggcggtcgtc
    36781 cataccttcg attttggcgg gtaaaacatc gcaagtgagg atgagctgtt ttttctcatt
    36841 gtgaaaatgg ttgtacagat agaaaaattc ttccatcgta cggtcttttc ctttgatgaa
    36901 ctgaatatcg tcgataatca gcaggtcgta ttgcttgtat tgctgcttga acacgtcgta
    36961 ggtattgttg cgaaccgcct tcataaagct gcggatgtag tcgtccgaat gcatatagcg
    37021 cactttggca tcgggacggt ttttcaacag ctcgttgccg accgcctgca caaggtgggt
    37081 tttgcccaaa cccgtgctgc cgtacaggaa gaacgggttg taactctgcc ccgggttttc
    37141 cgcaatcgcc tgcgccgcag ccgccgcaag gcggttgccc ttaccttcta ccaacgtatc
    37201 aaacgtgtaa tccggagaca ggttggtttg ttcgtaacgc gcttcttccg catcacgctg
    37261 cgcatccgtc cgcgctttgg caactgccgc cgattccggc cgggaagcag gcccggcagc
    37321 ctgacgcggc tcgtgcggca ggtttttcat acgttccgcc aaaatatccg ccgccgtttt
    37381 cgacgcagcg ggtttgacag gcttttcaga cggcagctcg tccaacagaa cctcctgcac
    37441 gggcatctcc tccgaccccg cgtgcaagga cggctcggca ggttcgacag caccttcaac
    37501 cgccgccatc tcataacgca cgccttctcc cggtttgaat acgaaggcgg gacggccggc
    37561 agccaactct tccctcaccg cttctatttt tccggcaaac tggctcttga gcatattgca
    37621 ggcaaactgg ttcttgccgt acaccaccca tacgccaccc tcctcaccaa ccgtaagggg
    37681 cgcaatccat tgcgcaaact gcccgtgagg caacatatcg tgaagacggc ggaggcacag
    37741 cggccaaaac tctgctaatg tcatggatag gctcgaatcg gtaaaaatga aatcgaaaac
    37801 aaagaaaata taatattttc aaaaagaaaa caaatctgtt gaatacgcgt cggttcaaaa
    37861 cgcagcgaac cgattatacc gactcgccga tattttatcc acaacccgtg caaaaattta
    37921 tccacagaaa ggcggcagaa atccgcaggc aatcgggcaa tcctcctgca aagtttctat
    37981 attgattgac aaaagcggca aattggagtg taattcacgg tttaattatc tacccattct
    38041 attttaggaa ccatcatgaa acgcacttat caaccttccg ttaccaaacg caaacgcacc
    38101 cacggcttcc tcgtgcgctc caaaacacgc ggcggacgcg cagtattggc cgcacgccgt
    38161 gccaaaggcc gcaaacgcct ggcagtgtaa ttttggacta ccgcttcgga aggcagtacc
    38221 gcttgttaaa aacggatgat ttttcatccg tttttgcatt cagaaaccgc cgcagccgcg
    38281 acctgctgca agtttcgcgc tcaaacggca acgggctgga ccatccccgc atcggtctgg
    38341 tggtcggcaa aaaaaccgcc aaacgcgcca acgagcggaa ctatatgaaa cgcgtcatcc
    38401 gcgactggtt cagattgaac aaaaaccggc tgccgcctca ggatttcgtc gtgcgcgtcc
    38461 gccggaaatt cgacagggct accgcaaaac aggcaagggc ggaactggca caactcatgt
    38521 tcggcaaccc cgcaaccgga tgcgggaaac aggtatgatc agaacggtac cctgcaggca
    38581 aggttcagac ggcaacgggt ttcccataca aggaacatcc cgatgaactt cctattgtcc
    38641 aaactgctgc tggggctgat aaggttttac caatactgca tcagcccgct gattccgccg
    38701 cgctgccgtt atacgccgac ctgttcgcaa tacgcggtcg aagcggtcaa aaaatacggc
    38761 gcattcaaag gcctccggct cgccatcaag cgcattgcac gctgccaccc tttcggcgga
    38821 cacggacacg accccgttcc ctgacccgac gcaatattca aattgcacgc tttcctttta
    38881 tttcccatcg gtttctatat aatgccgtct gaaccttcgg gctggcggca cggccgtcgg
    38941 gtatgaagcc cgcccttatt ccccgtctat cggaacacgc aacctgcggc atttccgacc
    39001 attcaggaaa ctctatatgg attttaaaag actcacggcg tttttcgcca tcgcgctggt
    39061 gattatgatc ggctgggaaa aaatgttccc caccccgaaa cccgtccccg cgccccaaca
    39121 ggcggcacaa aaacaggcag caaccgcttc cgccgaagcc gcgctcgcgc ccgcaacgcc
    39181 gattaccgta acgaccgaca cggttcaagc cgttattgat gaaaaaagtg gcgacctgcg
    39241 ccggctgacc ctgctcaaat acaaagcaac cggcgacgaa aacaaaccgt tcgtcctgtt
    39301 tggcgacggc aaagaataca cctacgtcgc ccaatccgaa cttttggacg cgcagggcaa
    39361 caacattctg aaaggcatcg gctttagcgc accgaaaaaa cagtacaccc tcaacggcga
    39421 cacagtcgaa gtccgcctga gcgcgcccga aaccaacgga ctgaaaatcg acaaagtcta
    39481 tacctttacc aaagacagct atctggtcaa cgtccgcttc gacatcgcca acggcagcgg
    39541 tcaaaccgcc aacctgagcg cggactaccg catcgtccgc gaccacagcg aacccgaggg
    39601 tcaaggctac tttacccact cttacgtcgg ccctgttgtt tatacccctg aaggcaactt
    39661 ccaaaaagtc agcttctccg acttggacga cgatgcgaaa tccggcaaat ccgaggccga
    39721 atacatccgc aaaaccccga ccggttggct cggcatgatt gaacaccact tcatgtccac
    39781 ctggatcctc caacctaaag gcggccaaag cgtttgcgcc gcaggcgact gccacatcga
    39841 catcaaacgc cgcagcgaca agctgtacag cgcaagcgtc agcgtgcctt tagccgctat
    39901 ccaagccggc gccaaagccg aaactgccgt caacctgtat gccggtccgc aaaccacatc
    39961 cgttatcgca aacatcgccg acaacctgca actggcaaaa gactacggta aagtacactg
    40021 gttcgcatcg ccgctcttct ggctcctgaa ccaactgcac aacattatcg gcaactgggg
    40081 ctgggcaatc gtcgttttga ccatcatcgt caaagccgta ctgtatccat tgaccaacgc
    40141 ctcctaccgt tcgatggcga aaatgcgtgc cgccgcaccc aaactgcaga ccatcaaaga
    40201 aaaatacggc gacgaccgta tggcgcaaca gcaagcgatg atgcagcttt acaaagacga
    40261 gaaaatcaac ccgctgggcg gctgtctgcc tatgctgttg caaatccccg tcttcatcgg
    40321 cttgtactgg gcattgttcg cctccgtaga attgcgccag gcaccttggc tgggctggat
    40381 taccgacctc agccgcgccg acccctacta catcctgccc atcattatgg cggcaacgat
    40441 gttcgcccaa acctatctga acccgccgcc gaccgacccg atgcaggcga aaatgatgaa
    40501 aatcatgccg ttggttttct ccgtcatgtt cttcttcttc cctgccggtt tggttctcta
    40561 ctgggtggtc aacaacctcc tgaccatcgc ccagcagtgg cacatcaacc gcagcatcga
    40621 aaaacaacgc gcccaaggcg aagtcgtttc ctaaatgccg cagcatgaaa aatgccgtct
    40681 gaaacctgtt cagacggcat ttttattgtt caccccctat cggggcggaa atcttcaacc
    40741 cgcatacatc acaaaaatcg tcgggcgttt tttcagattg ggcatttcct tcatcctgcg
    40801 ccaatccgca accgttttac tgaccaccaa ctgagtcggc aacgtcaaat ccgtagccgt
    40861 acacaagcgc gtttcaggat gcaggttttc caccgcatcg gcaagcagcg catcattgcg
    40921 gtaaggcgtt tcaataaaaa tctgcgtctc gccgcactgc cgcgaacgtt gctccaaagc
    40981 attcaaactc tgaatgcgct catttttttc agacggcaga taaccattaa acgcaaagtt
    41041 ctgcccattc gcacccgaag ccatcagcgc cagcagcagg ctggacgggc cgaccaaagg
    41101 ccgtacttca aaaccgtgtt tatgcgccaa tgccaccaaa ttcgcgcccg gatcggcaac
    41161 agccgggcaa cccgcctcgc tgacaatccc catactgcgc ccttcttgca aaggtttcag
    41221 caattccggc aaagtcttca aatccgtgtg ttcattcaac gtttgcagat tcagttcgcg
    41281 tatcggcgtc gttatcccca aatgcttcaa atgcgcacgc gccgtttttt ccgcttccac
    41341 gacaaaatcc gtcagcccga caaccgcccg ttgttcatgc tgcaacaggc acggcgtgtc
    41401 aggcgtaccc aaaggcgtag gaatcaaata caaaacagga gacatcattc cctcactcat
    41461 cggttaaaaa tgccgtccga acccttcaga cggcataaac gggcagttac aaaacatcca
    41521 cgccctcgtt tttcaaaaaa tcgaccagac ggaaaaccgg cagaccaatc aaggcattcg
    41581 gatcggtact ttcaatccgt tcgatcaaca gcgcgcccaa gccctcgctt ttcgccgcac
    41641 acgaacaata aaccgcatcc ggctcgcgct ccaaatagcg gaggatgtgc agctcgtcca
    41701 actgcctcat cacgaccacc gtcttatcga tatgccgatg catcctgccc gtaaccgtat
    41761 ttagcagaac gaccgcactg taaaactcaa tctccctgcc gctcaaatgc atcaacatct
    41821 tttgcgcgtt ggcaaggttc atcggcttgc cccactgcct gccgtcgcac cacgccacct
    41881 ggtccgcacc gacaatcaac gccccgggga aacgcccggt caacgaccgc gccttaccct
    41941 cggcaaggcg caatgccgtc tgaggggcgg attcccccaa catcggcgtt tcgtcaaaat
    42001 cgggggatgc cgcctgaaag gcaatgccga gcctttccat ctgttcgcgg cggaaaaccg
    42061 aactcgtacc caaaaccaaa ggcagttcca aacccatccc atcctcctta ccgttgaaaa
    42121 cacgcccgaa ggggcggtaa aatccagcca tgcgccgaaa cacggacgcc cgccttcggc
    42181 gtaccgcaac atttttctta aaaatattga cgttagaaca tctaaattat atcatacccc
    42241 gtttatgtca gaccctaatt tgattgaccc ggaaattttt gccgccgaaa ggcagaacct
    42301 gcaaggcagt tttctgctgg aagaattgga cgagcgagtc agtttgcacg attatcccgc
    42361 cgacaggcgg accaaaatat cgtttacact gaccggcggt cgcgaccggc tgcaacgcct
    42421 gttcctcgac ctgaacgtca aagccgatat gcccctgatt tgccagagat gtatcaaacc
    42481 catgccgttc atgctcgatg aaagcagccg tatcgtcctg ttttccgacg aagagtcctt
    42541 ggacgaatcc atgcttgccg acgaagaact cgaaggcata ctgattgaaa aagaactcga
    42601 cgtgcgcgca ttggtagaag accaaatcct gatgtccctg cccttttcgc cgcgacacgg
    42661 acactgcggc aatacccttc cggaatccgc caaccaagac aaacccaacc cctttgctgt
    42721 tttggcgggt ttgaaaagca gttaattagg acacagttta tttatctagg agcttgaaat
    42781 ggccgttcaa caaaacaaaa aatccccttc caaacgcggt atgcaccgtt cgcacgacgc
    42841 actgaccgcg cccgcactgt ttgtcgacag cacaaccggc gaagtacacc gcccgcacca
    42901 catctccccc aacggtatgt accgcggccg caaagtggtc aaagccaaag gcgaataatc
    42961 cctattcgac tgactgaaaa agccagaaca ttgccatgca attactggct ttttttacat
    43021 tggacgcacc atccgtccaa actttcgcca tacgtcaaca cacaggggca aagcgttccg
    43081 tataataccc cgtgaaaata ttccaaaagc cccaaccacc aaggaaattc cgatgaaaca
    43141 gaaaatctgg tacacctacg atgacatcca ccgcgtcatc aaagcattgg cagaaaaaat
    43201 ccggaacgcc ggcgtcaaat acgatgccat gatcgctatc ggcggcggcg gctttattcc
    43261 ggcacgcatg ctgcgctgtt ttctggaaat tccgatttat gccgtaacca ccgcctatta
    43321 cgacagcgac aacgaaggac aagttaccga agaagtcaaa aaagtccaat ggctagaccc
    43381 cgtccccgaa gtcctgcggg gcaaaaacgt gctcgtcgtc gatgaagtgg acgacagccg
    43441 cgtaaccatg gagttctgcc tgaaagaact gctcaaggaa gacttcgaca ctgtcggcgt
    43501 cgccgtcctg cacgaaaaaa tcaaagccaa agcaggcaaa atccccgaag gcattcccta
    43561 tttcagcggc attaccgtag aagactggtg gatcaactat ccgtgggacg cactcgacat
    43621 cgacgaacac aaccgccttg ccgaagccgg ccgaggctaa ccctttcaga cggcatattt
    43681 tccgaaccga tgccgtctga agcccgcacg acccctgccg cagaccgaaa atctaccgga
    43741 gaaaccccta tgattacatt ggccgtagat gccatgggcg gcgaccaagg acttgccgtt
    43801 accgtacccg gcgcaaccgc attcctccaa gcacaccccg atgtccgcct gattatgacc
    43861 ggcgacgaaa cgcagctccg ccaagccctg aacgcggcag gcgcgccgat ggaacgcatc
    43921 gacatctgcc ataccaccca ggttgtcggc atggatgaat cccctcagtc cgccctaaag
    43981 aacaaaaaat actcctccat gcgcgttgcc atcaaccaag ttaaagaagg caaagcccaa
    44041 gccgccgtat ccgcaggcaa cacgggcgcg ctcatggcaa ccgcacgttt cgtcctcaag
    44101 accatacccg gcatcgaacg ccccgccatc gccaaattcc ttccttccga caccgaccac
    44161 gttaccctcg ccctcgacct cggcgcaaac gtcgactgca cacccgaaca gcttgcccaa
    44221 tttgccgtca tcggcagcga actcgtccac gcactccatc ctcaaaaagg acagccgcgc
    44281 gtcgggctgg tcaacgtcgg cacagaagac atcaaaggta cggataccgt caaacaaacc
    44341 tacaaactgc tgcaaaacag caaactcaac tttatcggca acatcgaaag caacggcatc
    44401 ctgtacggcg aagcagatgt cgtcgtcgcc gacggctttg tcggcaacgt catgctcaaa
    44461 accatcgaag gcgcagtcaa attcatgagc ggagccatcc gccgcgaatt ccagagcaac
    44521 ctgttcaaca aacttgccgc cgttgccgcc ctacccgccc tcaaaggctt gaaaaacaaa
    44581 ctcgatccgc gcaaattcaa cggggccatc ctgctcgggc tgcgcggcat cgtcatcaaa
    44641 agccacggcg gcacagacaa aaccggtttc cgctacgcac tcgaagaagc ctaccacgaa
    44701 gccaagtccg ccggcctttc caaaatcgaa caaggcgttg ccgaacaact cgccgcactg
    44761 gaagccgccc aaaacgaaac cgccgccagt ctgtaacacg cacgatgccg tctgaacgcc
    44821 cccgcccctt tcagacggca tccggcaccc gtccaaacct gcgggcgcgg acggcgatgc
    44881 gcctgtccgg cacttcccaa atacttccct gtaaaataag gagtatttga aaaatgaaga
    44941 cattagaaaa acggatgaaa gctctagaca aacggatgat gaagttcgga aaatcccttg
    45001 aaggcaggct tgatgcccgt ctgattgaat ccgcattgga ttatattcat tattcggaac
    45061 gttttttggc ttttgacatc ctgtgtactt atatcgaaga tttcgatatc cggctgacgg
    45121 aacaagaatc ccgggaaatt tcttttatca acaaggaatt tgagatagaa agcacgtccg
    45181 attaaccaat aaagccaatg atttgataag aatgaaaaca tcgacggtcg tttttggcgg
    45241 attttttatg gcagacaacg gggagcgaat ccaaatcccc gttttggaaa atcctgacat
    45301 tagggaaatc aatcgctttt tttccgtatc aaattttgag aaaaaagccg gcgtccttgt
    45361 tttcagaatc atccccgagc cggaatttgg caataccgaa ttaactgtct attttgaaaa
    45421 aggatattat agtggattaa caaaaaccgg tacggcgttg ccccgccttg gctcaaagag
    45481 aacgattccc taaggtgccc aagcaccaag tgaatcggtt ccgtactatc cgtactgtcc
    45541 gcggcttcgc cgccttgtcc tgatttttgt taatccacta tatttgccaa taattcaaac
    45601 aattttagaa ggcggggata ttaaagtaaa aaaccttaaa acagaaaatt acagcgggaa
    45661 ggcaagggaa atcttgggag attcctaccc tattgaacat atctctaaaa atatttctac
    45721 aattcaaaat atcatttccg aatttatcag acaaaaacaa acaccggcac caaataattt
    45781 gatggaaacc caacaatcaa gatgccgtct gaagcagctt gggctttcag acggcatttc
    45841 cttcacttaa aacagcgtat cggcaacccc gccccgcccg tccacggcaa tccgcatctg
    45901 aaaaccatct gtatgccaaa ccacaccccc atccctgttt ccgtcatttg caccctgccc
    45961 gtattgggca atcatcggtt tttcgcttac aatagccgaa tctgaaccaa ctctctaaaa
    46021 aggccgttcc catgcagtat gcaaaaattt ccggcacggg cagctatctt cctgccaacc
    46081 gcgtcagcaa tgacgacctt gcccaaaagg tagatacctc tgacgagtgg attaccgcgc
    46141 gtacgggcat caaattccgc catattgcag ccgaaaacga aaaaaccagc gatcttgcag
    46201 ccgaagcggc gcggcgcgct ttggcagatg ccaaattaaa tataaacgac attgatttga
    46261 ttatcgtggc gacggcaacg ccggatatgc agtttccttc taccgccacc atcgtgcagc
    46321 aaaaattggg catcaccaac ggctgccccg cgtttgacgt gcaggcggtg tgcgcaggct
    46381 ttatgtacgc gctgactacg gcaaatgcct acattaaaag cggtatggcg aaaaacgcgc
    46441 tggtcatcgg cgcggaaacc ttcagccgca tcgtcgattg gaacgaccgc acaacctgcg
    46501 tgctgttcgg cgacggcgcg ggcgcggtgg ttttaagcgc gtccgacaaa ccgggcatca
    46561 tccacagcaa actcaaagcc gacggcaatt atctgaaact cttaaacgtc cccgggcaaa
    46621 tcgcctgcgg caaagtttcc ggttcgccgt acatttcgat ggacggtccc ggcgtgttca
    46681 agtttgccgt caaaatgctg tccaaaatcg ccgatgacgt tatcgaagaa gcaggttaca
    46741 ccgccgaaca aatcgactgg attgttcccc atcaggcaaa ccgccgcatt atcgaatcga
    46801 ccgcgaaaca tttgggtttg agtatggaca aagtcgtcct gaccgtccaa gaccacggca
    46861 acacgtccgc cgcatccatt ccgctggctt tggacgcggg catccgcagc ggacaaatca
    46921 aacgcggtca aaacctgctg ctcgaaggca taggcggcgg tttcgcgtgg ggcgcggtgc
    46981 tgttgcaata ttgaaaccgg tgccgtctga aacaggcttt cagacggcat ttcccatatc
    47041 atgaagcggc aggctttctt caaaccgatg gcgtgtgcgg catttctgtc cgccgtttcg
    47101 ctgcgcctcc ccgtattggg cgcgtgttac gcaatattgt ccctctatgc gtttgcactt
    47161 tacggcatcg acaaacggcg tgccgtgcgg ggaaaacgcc gcattcccga acaccgcctg
    47221 ctcctgcctg ccttgttcgg cggttggacg ggcgcatact tgggtagtag gatgttcagg
    47281 cataaaacgg cgaaaaagcg ttttgttgtg ctgttccgtc tgactgtttc gggcaatgtc
    47341 ctggcgacct gcatcctgat tgattatttc gttccgcccg aactttttgt aaaactcggg
    47401 caacatctct gacaatccaa cccaacaaag gaatactatg tctttcgcat tcttcttccc
    47461 cggacaaggt tcccaaagcc tcggcatgat gaacggcttt gccgaacacg ccatcgtcaa
    47521 aaacacattt gacgaagcct ccgccatatt ggggcaggac ttgtgggcga tgattaacgg
    47581 cagcgatgcc gaaatcatcg gtcaaacagt caacacccag cccattatgc tcgccgccgg
    47641 cgttgccgtt taccgcgcct atttggaagt gggcggcaaa acgcctgccg ccgttgccgg
    47701 acacagcctc ggcgaataca ccgcactcgt tgccgccggc gcattggatt ttgccgatgc
    47761 cgtcaaactc gtccgcctgc gcgccgaact gatgcagtcc gccgtaccgc aaggcgtagg
    47821 cgcaatggcg gccattttgg gtttggaaga tgaacaggtc aggcagattt gtgccgaatc
    47881 cgcacagggc gaagtggtcg aagccgtcaa cttcaactcg cccggacaag tcgtgattgc
    47941 aggcaacgcc gccgccgtcg gacgcaccat ggctgccgcc aaagaagccg gagccaaacg
    48001 cgccctgccg ctgccggtat ccgttccttc ccattgcagc ctgatgaaac ccgccgccga
    48061 caaacttgcc gaaacactca aaaccgttga aatcaagcag ccgcaaatcc gtgtcatcca
    48121 caacgccgac gttgccgcct acgatgatgc cggcaaaatc aaagacgcgc tcgtccgcca
    48181 gctttacagc cccgtgcgct ggacggaaac cgtcaacgcc ctcgtttcag acggcattgc
    48241 cgaatccgcc gaatgcggcc cgggcaaagt gttggcgggc ttggcaaaac gcatcaacaa
    48301 ggccgccgcg tgcagcgcac tgaccgatgc cggacagatt accgccttta tcgaagcgca
    48361 ctgacttcgt tccgcaaaaa gcagcctgcc ctcttcaggc tgcttttcac gtccgaacgg
    48421 caaacagccg catatttgcg ctataatcca tccctaccaa accaccgaca gcggctgccg
    48481 ttgcagttcc cgccctaccg atatgataga aaaactgact ttcggactgt ttaaaaaaga
    48541 agacgcgcgc agctttatgc gcctgatggc gtacgtccgc ccctacaaaa tccgcatcgt
    48601 tgccgccctg attgccattt tcggcgttgc cgccaccgaa agctatcttg ccgccttcat
    48661 cgcccccctg atcaaccacg gcttttccgc gcccgccgcg ccgcccgagc tgtccgccgc
    48721 cgccgacatc ctttccaccc tgcaaaactg gcgcgaacag tttacctata tggtttgggg
    48781 gacggaaaac aaaatctgga ccgtccccct cttcctcatc atcctcgtcg tcatccgtgg
    48841 catctgccgc tttaccagca cctatctgat gacttgggtc tccgtgatga ccatcagcaa
    48901 aatccgcaaa gatatgtttg ccaaaatgct gaccctttcc tcccgctacc atcaggaaac
    48961 gccgtccggc accgtactga tgaatatgct caacctgacc gaacagtcgg tcagcaacgc
    49021 cagcgacatc ttcaccgccc tcacgcgcga cacgatgatc gttaccggcc tgaccatcgt
    49081 cctgctttac ctcaactggc agctcagcct catcgtcgtc ctgatgttcc ccctgctctc
    49141 cctgctctcg cgctactacc gcgaccgctt gaaacacgtc atttccgact cgcaaaaaag
    49201 cataggcacg atgaacaacg tgattgccga aacccatcag ggacaccgcg tcgtcaagct
    49261 gttcaacggg caggcgcagg cggcaaaccg gttcgacgcg gtcaaccgca ccatcgtccg
    49321 cctcagcaaa aaaatcacgc aggcaacggc ggcacattcc ccgttcagcg aactgattgc
    49381 ctcgatcgcc ctcgccgtcg tcatcttcat cgccctgtgg caaagccaaa acggctacac
    49441 caccatcggc gaatttatgg cattcatcgt cgcgatgctg caaatgtacg cccccatcaa
    49501 aagccttgcc aacatcagca tccctatgca gacgatgttc ctcgccgccg acggcgtatg
    49561 tgcatttctc gacaccccgc ccgaacagga caaaggcacg ctcgcgccgc agcgtgtcga
    49621 agggcgcatc agcttccgca acgtcgatgt cgaataccgt tcagacggca tcaaagccct
    49681 cgacaacttc aacctcgaca tcagacaagg cgaacgcgtc gccctggtcg gacgttccgg
    49741 cagtggcaaa tccaccgtcg tcaaccttct gccccgcttt gtcgaaccgt ctgccggcaa
    49801 catctgcata gacggtatcg acatcgccga catcaaactc gactgcctgc gcgcccaatt
    49861 cgccctcgtc tcccaagacg tattcctgtt tgacgacacc ctgtttgaaa acgtccgata
    49921 cagccgtccc gacgctggcg aatccgaagt cctgtccgcc ctccaagccg ccaacctgca
    49981 aagcctgatc gacgcatccc cgctcggact gcaccagccc atcggatcga acggcagcaa
    50041 cttatccggc ggacagcggc aacgcgtcgc cattgcccgc gccattttga aagacgcgcc
    50101 gatattatta ttggacgaag ccaccagcgc attggacaac gaatccgaac gcctcgtcca
    50161 acaagcgctc gaacgcctga tggaaaaccg caccggcatc atcgtcgccc accgcctgac
    50221 caccgtcgaa tccgccgacc gcatcatcgt catggatggc ggcaaaatca tcgaacaagg
    50281 cacacacgat caactcatgt tccaaaacgg ctactacacc atgctgagga acatctccgg
    50341 aaaagatacc gccgccgttc agacggcata aacaaaatgc cgtccgaaat ggtataatcg
    50401 ccccgacccc ttcagacggc atcaagacgg catcatatcc gccgacccat ccgattatct
    50461 tcaaccaccg taaaacccat tatgacccaa gacaaaatcc tcatcctcga cttcggttct
    50521 caagttaccc ggctgattgc ccgccgcgtg cgcgaagccc acgtttactg cgaactgcat
    50581 tccttcgata tgcctttgga cgaaatcaaa gccttcaacc ccaaaggcat catcctttcc
    50641 ggcggcccta attctgttta cgaatccgac tatcaagccg ataccggtat ttttgatttg
    50701 ggcattccgg ttttgggcat ctgctacggc atgcagttta tggcgcacca cttgggtggc
    50761 gaagtgcagc ccggcaacca gcgcgaattc ggttacgcgc aagtcaaaac catcgacagc
    50821 ggactgacac gcggcattca agacgacgcg cccaacacac tcgacgtatg gatgagccac
    50881 ggcgacaaag tgtccaaact gcccgacggt ttcgccgtca tcggcgacac tccgtcctgc
    50941 ccgattgcca tgatggaaaa cgccgaaaaa caattctacg gcatccagtt ccaccccgaa
    51001 gtcacccaca ccaaacaagg ccgcgccctg ttgaaccgct ttgtcttgga tatttgcggc
    51061 gcgcaaccgg gctggacgat gcccaactac atcgaagaag ccgttgccaa aatccgcgaa
    51121 caagtcggca gcgacgaagt gattttaggt ctgtccggcg gcgtggactc ttccgtagcc
    51181 gccgcgctga ttcaccgcgc catcggcgac caactgacct gcgtgttcgt cgatcacggt
    51241 ttgttgcgcc tgaacgaagg caaaatggtg atggatatgt tcgcccgcaa cttgggtgtg
    51301 aaagtgatac acgtcgatgc cgaagggcag tttatggcga aactcgccgg cgtgaccgac
    51361 cctgagaaaa aacgcaaaat catcggcgcg gaatttatcg aagtatttga tgccgaagag
    51421 aaaaaactca ccaacgccaa atggctggcg caaggcacga tttaccccga cgtaatcgaa
    51481 tccgccggtg cgaaaaccaa aaaagcccac gccatcaaat cccaccacaa cgtcggcggc
    51541 ctgcctgaaa atatgaagct caaactgctt gagcccttgc gcgacttgtt caaagacgaa
    51601 gtgcgcgagt tgggcgtggc tttgggcctg ccgcgcgaaa tggtgtaccg ccaccccttc
    51661 ccgggccccg gtttgggtgt gcgcatcttg ggcgaagtga aaaaagaata cgccgacttg
    51721 ctgcgtcagg cggacgatat tttcatccaa gaattacgca atactaccga cgaaaacggc
    51781 acgtcttggt atgacctgac cagccaggca tttgccgtat tcctgcccgt caaatccgtc
    51841 ggcgtgatgg gcgacggccg cacttacgac tacgtcgtcg cactgcgcgc agtcatcacc
    51901 agcgacttta tgactgcaca ctgggcagag ctgccatact cactgctcgg ccgcgtgtcc
    51961 aaccgcatca tcaacgaagt caaaggcatc aaccgcgtgg tgtacgatgt cagcggcaaa
    52021 ccgcccgcca ccatcgagtg ggaataaaca gcaaacaggg ctgcaccgtc cggcgcagtc
    52081 cttcgattat cggaaaaaag gaaaaaatat gagcacacaa gatttaagcg gcaaaatcgc
    52141 tttggtaacc ggcgcatcgc gcggcatcgg cgcggcaatt gccgacacac tggcggcagc
    52201 cggtgccaaa atcatcggta cggcaaccgg cgagagcggt gcggcggcga ttagcaaacg
    52261 tctggcacaa tggggcggcg aaggccgcgt attaaattcc gccgaacccg aaaccgtcga
    52321 aaacctgatt gccgacatcg aaaaaacgtt cggcaaactc gacattctgg tcaacaacgc
    52381 cggcatcacc cgcgacaacc tgttgatgcg tatgaaagaa gaagagtggg acgacatcat
    52441 gcaggtcaac ctcaaatccg tgttccgcgc ttctaaagcc gtcttgcgcg gtatgatgaa
    52501 gcagcgcgcc ggccgcatca tcaacatcac ctccgtcgtc ggcgtgatgg gcaatgccgg
    52561 gcaaaccaat tatgccgccg caaaagcggg cttgatcggt tttgccaaat ctatggcgcg
    52621 cgaagtcggc agccggggca ttaccgtcaa ctgcgtcgcc ccgggcttta tcgataccga
    52681 tatgacccgc gccctgcccg aagaaacccg ccaaacattt accgcccaaa ccgccttggg
    52741 cagattcggc gacgcgcaag acatcgccga tgcggttctg ttcctcgctt ccgatcaggc
    52801 gaaatacatc accggtcaaa cgctgcacgt caacggcggc atgctgatgc cctaacggac
    52861 aggcttttca accatgccgt ctgaagccct ttcagacggc atttgcattc ccaaacaaaa
    52921 tgaacactca ccaccacccg ccctgcccct gcggttcagg cacaagctat gccggctgct
    52981 gccgaccgct tcatctgcgc caaatcctgc cgcccaccgc cgaagccctc atacgttcgc
    53041 ggtacggcgc gtatgtgctg caccttatcg actacatcat cgctactacc gttccggcgc
    53101 aacaaacatt cctcgatgcc gccgaactga tgcagtggag cagagaagcc gaatggctgg
    53161 gcttgaatgt catcgcacac cgcaacctcg gcaaacagca cgcccaagtc gaatttgaag
    53221 cctacttcca agacggcgga aaccgaacag tgcatcacga gctgtcggca ttcgtgaaaa
    53281 tagcggagca atggtatttc atcgatccga ctgttccgct gcctaccatg aaacagccct
    53341 gcatttgcgg ttcgggcaaa aaattcaaag cctgctgcgg caaatatctg caacctgtcg
    53401 cataaaaatg ccgtctgaac gttcagacgg cattttcaac gcgcaaaaaa aaaccattca
    53461 taccaagggt aagtatgaat ggtcaataca ttgcgggaaa acatcttact tgctgcactg
    53521 ccgaaaaggg agaaacggca gtgttaacca gcaaaagcat tgtaccttta ttaatgttaa
    53581 aaaacattaa ttttgaaaat atattaacga atatatctgg aacctactgg ttttcccgta
    53641 tccacccgac ccagcgttca aacagcttcg gttcgagcgc ggcgacgacc gagcgtttga
    53701 acacgtgttc accactccaa aacccgtcgc cttccaaagt cgtcagcctg ccgcccgcct
    53761 cctcgaaaat caacgcaccc gcagcataat cccacagctt ctgcccgccg tggacataaa
    53821 catcataacg cccgcacgcc agataacacc agtccagcgt gctgctgccc atactgcgta
    53881 tcgtcccgaa aggcgcgagc gtactcattc ggctggaaag tttgcccgaa cgcagatatt
    53941 tgatttccac gcccgcaatc gcctcattga gttttttatc cacgagacgc aaaggcaggc
    54001 gtgtcccatt aagaaacgca ccctgcccgc gttcggcata aaaacattcg ccgctgaccg
    54061 gattgtagat tacgcccaat tcggcgcgcc cgttgcggac aaacgccacc gacacggcaa
    54121 aatgcggcag cccgttgaca aaattgttcg tcccgtctat cggatcgaca atccacagcc
    54181 ccttttcccc cgaatattgt tcccacaaag ccgactgttc ctgccgcgac atttcctcgc
    54241 ccaacatcgg gcaatcgatc agaagcggca acgcggcggc aaatgccgtc tgcgcggcaa
    54301 tgtccgcctc gctcaacatc gaaccgtctt ccttgcggcg ggacggcgta ttcaaaaaac
    54361 gcggcataat ttcggtttgc gcgatatggc gcacgacttt ctgcaaacgg tgtaacactt
    54421 cctactgtcc tcatattttg aacttgcggc gcgcgaacgt ataatgtccg tttccatcac
    54481 gccgctgcga cggattataa ccgtccgaac cgccaaaaac tatgcccaga ttctacctgc
    54541 ccgaaaacct ttccgtcgga caaaccgtcg accttcccga caacatcgtc cgccacctca
    54601 acgtcctgcg catccgcccc aacgaaaaca tcaccctctt cgacggcaaa ggcaaggcac
    54661 ataccgcacg gctgaccgtt ttggaaaaac accgcgccga agccgaaatc ctgcacgaag
    54721 acacaaccga caacgagtcc ccgctcaaca tcacgctcat ccaatccatt tcctccggcg
    54781 atcgcatgga tttcaccctg caaaaaagcg tcgaactcgg cgtaaccgcc atacagcccg
    54841 tcatcagcga acgctgcatc gtccgcctcg acggagaacg cgccgccaaa cgcctcgcac
    54901 gctggcagga aatcgtcatc tccgcgtgcg aacaaagcgg caggaacacc gttccccccg
    54961 tactgcccat catcggctac cgtgaagcac tcgacaaaat gccgtctgaa agcaccaggc
    55021 tgattatgag catcaaccgc gcctgcaaac tcggcgacat ccgccacccg tccggcgcaa
    55081 tcgtctttat ggtcgggccc gaaggcggct ggacagaaca ggaagaacaa caggcatttg
    55141 aagccggctt tcaggcggtt acactcggaa aacggatttt acgcacagaa accgccccac
    55201 tcgccgccat cgccgcgatg cagacgcttt ggggcgattt tacctagcag atgaaaaaac
    55261 acgggatttt atccgaaaaa gcaacaatcc ccccgccgtc atccccgcga aagcgggaat
    55321 ctggaatttt aatgcggcaa gaatttatcg gaaaaaaccg aagtttaaag acctagattc
    55381 ccgcctgcgc gggaatgaca gtgtgtccat ttctgatttt aatcccctat attttacaca
    55441 aactatttga acaatatgac ccgcctgccg taaactttct caagctccgc ctgcctttga
    55501 cgctccattc ttttcttctt ttccctaccg aatttaccca aagcacgctt caagtcaaac
    55561 atcaccttca acgaacggcg gtgtcttctt ccttgttccc tatctttttc caaatcgcta
    55621 cccaacatac tgttttgact gagaaacttg gcataatgca attcttggat acataaggcg
    55681 ggactaacct gataaacagg catcccctcc ttatcaaaga aataagtaaa catcatcaaa
    55741 tctaccgctt taatccgctc tggcggcaaa acggcaaacc tgtccaagaa aaaccgcatc
    55801 gcctcacgcg aaatgatata gccagccgtc ccacaatgtt cgctctccag caaaggaaat
    55861 gaccggtttt cataattcag gactttatcc ggtctgacaa taactttcgc aaacatcgtt
    55921 tccaaacgga cgatgaaggc ggaatcaggg tcaaagcgtt cttgcagcca agtatcttcg
    55981 gcaaggaact gctccgcgcc ttcgccgagt aaaacgtcgt cctcaaatac ggcgatatac
    56041 ggcagacctt catccaatgc ctgttcccac aatacggcgt ggctcataaa gcaggctttt
    56101 tccactccgc tcaaataggg gtgcgccgac aagccgggga cgagttccgc catcgcccgt
    56161 tccagccttt cagacggcat cagtgcgtcg aaaaactgga acgggatgcc gcgactgccg
    56221 aaggtatcgg caatgtgcgc cctgcgctct gcggcggaag ccaagctgat aacgtggttt
    56281 tgcatgattt atcctgttcg gtttcaatag ctgcggtatt tccaagggcg taacattttt
    56341 ttcaggattt tcctatattg cggtgcggca aacagcttcc tcatcacgcg gtctgtcaag
    56401 aaatccagca aatcggtcaa agaatacttt tccaacgaca agaaatattc gtacaggaac
    56461 aggcggaggc atccgatatc ctgtccggac aaggcttttt cgtacaacgc atatgccgtt
    56521 gatttcaaaa gcccgtaatt caggcagtcc gccccgacgg ctatgcctgc cgccttccaa
    56581 taccccgccc tgatttcttc tttgattttc cacgccgtcc tgcgctgttg caggttgtat
    56641 ttggaagaag tctggtcttg atgtaagcgg tatttgacca aggcttcggg ataataagcc
    56701 agcctgccca gtttgccggc ttcgtaccaa aacttatagt cttcggcgtg gatataggct
    56761 ggatcgaacc gcaaaccgcc gtcaatgacg ctgcgcctca taatcatcgt gttgttgtgt
    56821 atggggttgc cgaaagggaa aacggcgaca atgtcttcat gccgggtcgg tttgtcccaa
    56881 attgcgccgt ttcgggcaat ggcggcaagc acgcttttat tgttttcttc cgacaaaact
    56941 tccaaccacg cgcccatcgc aatgatgctg cggtcttttt ccatctcgcc cacgattttc
    57001 tcaatccagc cgggggaggc aatatcgtcg gcatcggtgc g
//
LOCUS       DBJGGI010000005        54959 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.005, whole genome shotgun sequence.
ACCESSION   DBJGGI010000005 DBJGGI010000000
VERSION     DBJGGI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 54959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54959
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.005"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            721..984
                     /locus_tag="ACSIGJ_000299"
     CDS             721..984
                     /locus_tag="ACSIGJ_000299"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329279.1"
                     /translation="MSSAQQANYPQNLFPTKNNPQVCTKGNPPYRHPFKTAIQMILKI
                     KKSYPQKIPHINIIIVLLFKPYILFITLQGNGTAKRKGIFSKN"
     gene            1064..2512
                     /gene="gnd"
                     /locus_tag="ACSIGJ_000300"
     CDS             1064..2512
                     /gene="gnd"
                     /locus_tag="ACSIGJ_000300"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HHQ1329280.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKETGIIGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFVEQLLPLLEEGDIIIDGGN
                     ANYPDTTRRTHYLAGKGILFVGAGVSGGEEGARRGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLAEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTATPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWGLDYGNTALLWREGCIIRSAFLGNIRDAYEANPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2575..3846
                     /gene="waaA"
                     /locus_tag="ACSIGJ_000301"
     CDS             2575..3846
                     /gene="waaA"
                     /locus_tag="ACSIGJ_000301"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HHQ1329281.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPHP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRRRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEDMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLAVVPRHPEHFQTTFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVGSGCQNII
                     EPLSCGVTTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKNLSGEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3887..4336
                     /locus_tag="ACSIGJ_000302"
     CDS             3887..4336
                     /locus_tag="ACSIGJ_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HHQ1329282.1"
                     /translation="MSIYAVAHIIHLYCATAFVGGVFFEVLVLSVLHTGRVSREARRE
                     VEKAMSYRAVRVMPFAVGLLFASGIVMAANRYLPISGEPFATSFGTMLTLKILLAFSV
                     LAHFAVAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            4369..5343
                     /locus_tag="ACSIGJ_000303"
     CDS             4369..5343
                     /locus_tag="ACSIGJ_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HHQ1329283.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGIKET
                     LAWSGLWVAVSCLFAGWLYFELAGNPGYGAAAAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVRQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEEDLANSRLLNAVKKVVPVGTAFHGEKFFTVENGKKIATPLFLVLIMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            5412..5487
                     /locus_tag="ACSIGJ_000304"
     tRNA            5412..5487
                     /locus_tag="ACSIGJ_000304"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5445..5447,aa:Lys,seq:ttt)
     gene            5708..6961
                     /gene="murA"
                     /locus_tag="ACSIGJ_000305"
     CDS             5708..6961
                     /gene="murA"
                     /locus_tag="ACSIGJ_000305"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HHQ1329284.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVATTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVNQHLKGLEAMGAEIAIEHGYVKAKGKLKGARVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGA
                     GELYGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVERIY
                     HLDRGYENIEKKLGSVGANIERVSG"
     gene            7042..8235
                     /locus_tag="ACSIGJ_000306"
     CDS             7042..8235
                     /locus_tag="ACSIGJ_000306"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HHQ1329285.1"
                     /translation="MAFLKLTEQNVRGKTVLIRADMNVPFKGGKISDDTRIRASLASV
                     KYCLDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYAALCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALAELLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTEQIGYISTGGGAFLEFLEGKELPAVAALEKTRR
                     VNGLI"
     gene            complement(8287..8535)
                     /locus_tag="ACSIGJ_000307"
     CDS             complement(8287..8535)
                     /locus_tag="ACSIGJ_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HHQ1329286.1"
                     /translation="MLTPEQVKALIAGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8628..9545)
                     /gene="ftsX"
                     /locus_tag="ACSIGJ_000308"
     CDS             complement(8628..9545)
                     /gene="ftsX"
                     /locus_tag="ACSIGJ_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HHQ1329287.1"
                     /translation="MSIIHYFSLHVESARSALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLTRDKRLDNIRFIGKEDG
                     LAELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSIFSAAVSLGLCGWLLSAVHPLVDAIFKPYGLNIGWRFFYVGELG
                     LVFGFVIALGVFGAWLATTQHLLCFKAKK"
     gene            complement(9542..10192)
                     /locus_tag="ACSIGJ_000309"
     CDS             complement(9542..10192)
                     /locus_tag="ACSIGJ_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HHQ1329288.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSMGKVLFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILNDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(10387..10869)
                     /locus_tag="ACSIGJ_000310"
     CDS             complement(10387..10869)
                     /locus_tag="ACSIGJ_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HHQ1329289.1"
                     /translation="MKRLNLAAIALAATFAAHTASADELAGWKDNTPQNLQSLKAPVR
                     IANLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKSYGNNVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            10993..11346
                     /gene="arsC"
                     /locus_tag="ACSIGJ_000311"
     CDS             10993..11346
                     /gene="arsC"
                     /locus_tag="ACSIGJ_000311"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HHQ1329290.1"
                     /translation="MSEIKIFHNPRCSKSRAAVSLLEERGIAAEAVKYLDTPPDLSEL
                     KDIFNKLGLESARGMMRVKDDLYKELGLDNPDLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            11343..11999
                     /locus_tag="ACSIGJ_000312"
     CDS             11343..11999
                     /locus_tag="ACSIGJ_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HHQ1329291.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            12115..13509
                     /gene="gltX"
                     /locus_tag="ACSIGJ_000313"
     CDS             12115..13509
                     /gene="gltX"
                     /locus_tag="ACSIGJ_000313"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HHQ1329292.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNADNVVYQTRRFDRYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCAVVDDYDMGVTHIIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTTEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPDGKLTELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAERLEGLEDWNAKAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALISKEEVLKRIRA"
     gene            13530..13925
                     /locus_tag="ACSIGJ_000314"
     CDS             13530..13925
                     /locus_tag="ACSIGJ_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329293.1"
                     /translation="MPSDVGMRLQTAFRRKLNMKKILYFLMFVFSTSVWAGGAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEEMRQIGVRLDHAWNAVYGAGERKRRGN"
     gene            13929..14420
                     /locus_tag="ACSIGJ_000315"
     CDS             13929..14420
                     /locus_tag="ACSIGJ_000315"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHQ1329294.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDSGTLLGSAAVKRHDMENFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDV
                     DKV"
     gene            complement(14696..17290)
                     /gene="mutS"
                     /locus_tag="ACSIGJ_000316"
     CDS             complement(14696..17290)
                     /gene="mutS"
                     /locus_tag="ACSIGJ_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HHQ1329295.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYELFLDDAVEAA
                     KLLDITLTTRGQMDGVPIKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTADK
                     LNDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDSKEHAVSIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKTPTLFSILDGCATHMGSRLLALWLHHPLRNRAHIRARQEAVTALESQYEPL
                     QCHLKSIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSATGSSLLETLKAVFP
                     ETLPVAETLKAAVMPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQDQALALEKQLFDGVLKNLRTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVVHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPVDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSI
                     VLMDEVGRGTSTFDGLALAHAIAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGRDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKAAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVDCFVDKAEEKHFEGILAAALENLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17721..18725
                     /gene="gap"
                     /locus_tag="ACSIGJ_000317"
     CDS             17721..18725
                     /gene="gap"
                     /locus_tag="ACSIGJ_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HHQ1329296.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHGIEVAAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGREIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPATKEEINAAMKAASSESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18877..19557)
                     /gene="tsaA"
                     /locus_tag="ACSIGJ_000318"
     CDS             complement(18877..19557)
                     /gene="tsaA"
                     /locus_tag="ACSIGJ_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HHQ1329297.1"
                     /translation="MTYTITPIGAARSPYKQKFGIARQPGLVSAAEACIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGSDLLDGTPIVDIKPYIPFIESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     INLSPTPL"
     gene            20017..20652
                     /locus_tag="ACSIGJ_000319"
     CDS             20017..20652
                     /locus_tag="ACSIGJ_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HHQ1329298.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMAAKWLVGNPVDMLKPTGLSDADLTWVAHAMVGTRGYELLDGLPSVKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTNMVIVGGLATDYCVKTTVLQL
                     LKGGRWQVIVNEAACRGIAPDTIEAAWQEMRSSGAIILKNAEKIKKYINNQ"
     gene            20689..21657
                     /gene="waaC"
                     /locus_tag="ACSIGJ_000320"
     CDS             20689..21657
                     /gene="waaC"
                     /locus_tag="ACSIGJ_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HHQ1329299.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCSDVELHWLCEAGFAGI
                     ARLHPFVKKVHVMKWRQWRKHLFQAETWREMGRLKQALLQEAFDFVLDSQGLIKSACF
                     AKMAKSPIYGLDKHSAREGVAALAYDKKYVVPKGRNAVWRNRDLFAQVFGYAMPETQV
                     FGLSVPEASRLKNLAQPYYAALHATSRDSKLWPMENWRELLQKLNQKQQCNVYLPWGN
                     EAEKVRAEQIADGLPFTIVCAKMNLLQAAYLLKHAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSAIAKNVGNIGQIPTADLVYQTLMDCVSADKG"
     gene            21883..22632
                     /locus_tag="ACSIGJ_000321"
     CDS             21883..22632
                     /locus_tag="ACSIGJ_000321"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HHQ1329300.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHIETDVKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFVSKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLDSADLATDISPRLKTV
                     KFAEPKARQTGVKVASVAELVEKLKNEAKVI"
     gene            22643..23578
                     /locus_tag="ACSIGJ_000322"
     CDS             22643..23578
                     /locus_tag="ACSIGJ_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HHQ1329301.1"
                     /translation="MSVLIIAEHDNKQLNPATLHAVAAAAKLGKVDLLVVGNGASSVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRVTAFDAVAAQGGN
                     AEVINVKTTPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSVEKFSEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLIELLKN"
     gene            23641..24252
                     /locus_tag="ACSIGJ_000323"
     CDS             23641..24252
                     /locus_tag="ACSIGJ_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HHQ1329302.1"
                     /translation="MITTYKTITSPTQAEFKDKGSRFIAFAYPIRTLADVKKYLNLLK
                     EEHYKARHWCYAYRLGVNGMQFRGNDDGEPPGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGALLGVPGLIHAYKEATAQALAIAEVVEKNIEKTVWLKCEYPFLNEAICIAKQYQAD
                     ILEQDLQLDCRLTVSLSLVNYEACVAAWKNTRQVEVNTEKPFE"
     gene            complement(24304..24642)
                     /locus_tag="ACSIGJ_000324"
     CDS             complement(24304..24642)
                     /locus_tag="ACSIGJ_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HHQ1329303.1"
                     /translation="MKKEQANTQKRIDEIQSKYREWCHLLLQLEENIRRWKYAVALIR
                     DMDNFYTHEYQVRHQTIEDGVELNLQTEGEYSIMSEDALWNAPGEFHQLAWLYLCSSV
                     DTLDRYTQEN"
     gene            24779..26050
                     /gene="purD"
                     /locus_tag="ACSIGJ_000325"
     CDS             24779..26050
                     /gene="purD"
                     /locus_tag="ACSIGJ_000325"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HHQ1329304.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIESKLQ
                     NIALTAYQDLIEFCRKENIVFTVVGPEAPLAAGIVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMVKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLVAGKGVIVAMTLD
                     EAHAAIDDMLLGNKMGNAGERVVIEDFLQGEEASFIVMVDGNHVLPMATSRDHKRLLD
                     GDKGPNTGGMGAYSPAPVVTPAVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLADLVEAAIDGRLDSVKAEWNPQTAVGVVL
                     AAQNYPETPKKGDVISGLDDVNRIGKVFHAGTTVNEKGDVLTNGGRILCVVGLGDDVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            26084..26686
                     /locus_tag="ACSIGJ_000326"
     CDS             26084..26686
                     /locus_tag="ACSIGJ_000326"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HHQ1329305.1"
                     /translation="MTDALQTAFYIMLFSRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQLEQLQPLLQMPFENIQTMLRNEWPA
                     PKTFLLLAKSGVLPALRGKRRSKLAVRVPDHTGARRLCQALGMPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            26701..27216
                     /locus_tag="ACSIGJ_000327"
     CDS             26701..27216
                     /locus_tag="ACSIGJ_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329306.1"
                     /translation="MKTFLHTQRKNFLDGISLHDCYRTAIKVEGRNVCFDFEDGFVVL
                     DNNPNNQAGKHLKTDFSRVVLTHENRNAEDDYLVDIFEDIVFLGKRLFTVRKFLDFSE
                     LLEMINAQGYFLEFLYLYECIGVKTSDYFLEAVLVTGRSRECKKCFIKIMRASSFVYQ
                     WNNLRLNSEIV"
     gene            complement(27368..27808)
                     /locus_tag="ACSIGJ_000328"
     CDS             complement(27368..27808)
                     /locus_tag="ACSIGJ_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HHQ1329307.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
     gene            complement(28040..28219)
                     /locus_tag="ACSIGJ_000329"
     CDS             complement(28040..28219)
                     /locus_tag="ACSIGJ_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HHQ1329308.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYMHLLFCPHCREYKRQL
                     QTIKISLAKTTRTSK"
     gene            complement(28225..28812)
                     /locus_tag="ACSIGJ_000330"
     CDS             complement(28225..28812)
                     /locus_tag="ACSIGJ_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HHQ1329309.1"
                     /translation="MPLPDLTDAELMESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTALDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSNEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            complement(28834..29580)
                     /locus_tag="ACSIGJ_000331"
     CDS             complement(28834..29580)
                     /locus_tag="ACSIGJ_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HHQ1329310.1"
                     /translation="MQPETSAQYQHRFSQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSKEWSRLKEGFVRDARAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTHDLQEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPKADNWKNILLGKWSG
                     WIEQRIIIPSLSAISENMEGNSPSQNHLSA"
     gene            complement(29570..30412)
                     /locus_tag="ACSIGJ_000332"
     CDS             complement(29570..30412)
                     /locus_tag="ACSIGJ_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HHQ1329311.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGRAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMCRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLENVDAGRVCYIHIAGHDAETPELLIDT
                     HGAAVLPTVWDLLELAYTKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            complement(30530..30838)
                     /locus_tag="ACSIGJ_000333"
     CDS             complement(30530..30838)
                     /locus_tag="ACSIGJ_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329312.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAAHKPASNATGVQKSAQGSCGA
                     SKSAEGSCGAAASKAGEGKCGEGKCGATVKKAHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            complement(30912..31361)
                     /locus_tag="ACSIGJ_000334"
     CDS             complement(30912..31361)
                     /locus_tag="ACSIGJ_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HHQ1329313.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNFAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKLFCPKCRLKQD"
     gene            31701..32594
                     /locus_tag="ACSIGJ_000335"
     CDS             31701..32594
                     /locus_tag="ACSIGJ_000335"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HHQ1329314.1"
                     /translation="MVTFSKIRSFLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVA
                     LALGGGASKGFAHIGIVKVLKENGIPVKVVTGTSAGSIVGSLLASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHKYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PSKNVGQGFFSYLDQTLNVMSVSVLQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            32697..33800
                     /gene="prfB"
                     /locus_tag="ACSIGJ_000336"
     CDS             32697..33800
                     /gene="prfB"
                     /locus_tag="ACSIGJ_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HHQ1329315.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKESKILEGIVLTLDNIASGIEDNRMLIEMAVEENDEEGFAAVK
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            33879..34238
                     /gene="rnpB"
                     /locus_tag="ACSIGJ_000337"
     ncRNA           33879..34238
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACSIGJ_000337"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(34565..36022)
                     /locus_tag="ACSIGJ_000338"
     CDS             complement(34565..36022)
                     /locus_tag="ACSIGJ_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHQ1329316.1"
                     /translation="MSRHPARTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGITLIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISSGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
     gene            complement(36804..40973)
                     /locus_tag="ACSIGJ_000339"
     CDS             complement(36804..40973)
                     /locus_tag="ACSIGJ_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HHQ1329317.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPAPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWIAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITDISAGDIAIVTKPTPPKEERPPQGLPDSIDLPA
                     TVYLDRFETGKISMGKTFDKQTVYLERLNAAYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGFEGETIHSTARLSGSLKDVRAELTIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KADFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGTGTARTDGSLAIASDPAN
                     EQRKLVFDTVNISAGEGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQFPAGNING
                     SIHLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIVKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGTARN
                     LHIGKAADIRSLDFTLKGSPDTSRPMRADIKGGRLSLSGGAAVVDTAGLTLEGTGAQH
                     RIRTHAAMTLDGKPFKLDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDRKTGISAKGGARGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGHNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIGNAFGGNMANTPLGGRITASLPDLGALKPFLPAAAQNITGSLNASAQ
                     IGGRVSSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVSMENSVPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAASLPVNMNLTLDLN
                     DGIRFSGYGADVTIGGKLTLTAQPGGNVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYGISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRLFGSD
                     KKDSAGNGKGK"
     gene            complement(41028..42875)
                     /locus_tag="ACSIGJ_000340"
     CDS             complement(41028..42875)
                     /locus_tag="ACSIGJ_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0097347 - TAM protein secretion complex
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HHQ1329318.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     SKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQTVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYQGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDVDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            43247..44476
                     /gene="sstT"
                     /locus_tag="ACSIGJ_000341"
     CDS             43247..44476
                     /gene="sstT"
                     /locus_tag="ACSIGJ_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HHQ1329319.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALAVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            44627..45907
                     /locus_tag="ACSIGJ_000342"
     CDS             44627..45907
                     /locus_tag="ACSIGJ_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HHQ1329320.1"
                     /translation="MFKRSVIAMACIFPLSACGGGGGGSPDVKSADTPSKPAAPVVAE
                     NAGEGVLPKEKKDEEAAGGAPQADTQDATAGEGSQDMAAVSAENTGNGGAATTDNPKN
                     EDAGAQNDMPQNAAESANQTGNNQPAGSSDSAPASNPAPANGGSDFGRTNVGNSVVID
                     GPSQNITLTHCKGDPCNGDNLLDEEAPPKSEFESLSDEEKIKKYKKDGEKFTGLVAIK
                     VENNGLNKYTIIYQAQPTRSARSRRSLPAEIPLIPVNQADTLIVDGEAVSLTGHSGNI
                     FAPEGNYRYLTYGAEKLPGGSYALRVQGEPAKGEMLVGTAVYNGEVLHFHMENGRPYP
                     SGSRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGRF
                     YGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKDRD"
     gene            45907..46110
                     /locus_tag="ACSIGJ_000343"
     CDS             45907..46110
                     /locus_tag="ACSIGJ_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HHQ1329321.1"
                     /translation="MLMPPEAQVFITAGRRVQIIKQAPEKGGIPKSGTEIAARIGGIP
                     QPAGETVGSSRNVGYFRPPFSGC"
     gene            46170..46418
                     /locus_tag="ACSIGJ_000344"
     CDS             46170..46418
                     /locus_tag="ACSIGJ_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329322.1"
                     /translation="MFQHSAGSNRQQQGDNALNPALRGGRVRAGLGKGIAFPNRGGAT
                     GCALCAERVCGNGMPSEAGAFRRHCDVWGAGPVQIRIS"
     gene            complement(46411..46626)
                     /locus_tag="ACSIGJ_000345"
     CDS             complement(46411..46626)
                     /locus_tag="ACSIGJ_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329323.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDNGFIN"
     gene            complement(46619..46834)
                     /locus_tag="ACSIGJ_000346"
     CDS             complement(46619..46834)
                     /locus_tag="ACSIGJ_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HHQ1329324.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(47037..48380)
                     /gene="argG"
                     /locus_tag="ACSIGJ_000347"
     CDS             complement(47037..48380)
                     /gene="argG"
                     /locus_tag="ACSIGJ_000347"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HHQ1329325.1"
                     /translation="MNQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIEPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            48505..49305
                     /locus_tag="ACSIGJ_000348"
     CDS             48505..49305
                     /locus_tag="ACSIGJ_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HHQ1329326.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDRTRQTAAAALDLPVVRHPEAAKFIEG
                     ITRKRGKPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYAGRFNQTERGSRSVYVFEQPESRITPIIEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAKLKNRVN"
     gene            49345..50331
                     /locus_tag="ACSIGJ_000349"
     CDS             49345..50331
                     /locus_tag="ACSIGJ_000349"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HHQ1329327.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDIMREVLLSAYRDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGKIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVSEALQAVR"
     gene            50413..50896
                     /locus_tag="ACSIGJ_000350"
                     /pseudo
     CDS             50413..50896
                     /locus_tag="ACSIGJ_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685638.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            50992..51243
                     /locus_tag="ACSIGJ_000351"
                     /pseudo
     CDS             50992..51243
                     /locus_tag="ACSIGJ_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(51238..51434)
                     /locus_tag="ACSIGJ_000352"
                     /pseudo
     CDS             complement(51238..51434)
                     /locus_tag="ACSIGJ_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            51536..51897
                     /locus_tag="ACSIGJ_000353"
                     /pseudo
     CDS             51536..51897
                     /locus_tag="ACSIGJ_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(51935..52309)
                     /locus_tag="ACSIGJ_000354"
     CDS             complement(51935..52309)
                     /locus_tag="ACSIGJ_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HHQ1329328.1"
                     /translation="MIIQNEFNLYPSNMLPEGFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFAGNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEEWLELAIKDTW"
     gene            complement(52483..52839)
                     /locus_tag="ACSIGJ_000355"
     CDS             complement(52483..52839)
                     /locus_tag="ACSIGJ_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP12 family DNA mimic protein"
                     /protein_id="HHQ1329329.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMVDWAYENIQSETVVEITENQI
                     IEYQNRGLWGLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(52836..53684)
                     /gene="mafB"
                     /locus_tag="ACSIGJ_000356"
                     /pseudo
     CDS             complement(52836..53684)
                     /gene="mafB"
                     /locus_tag="ACSIGJ_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(53785..53994)
                     /locus_tag="ACSIGJ_000357"
     CDS             complement(53785..53994)
                     /locus_tag="ACSIGJ_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329330.1"
                     /translation="MKLIDFEGNLVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKNKVLLLDKELKKIYDTWDKC"
     gene            complement(54071..54430)
                     /locus_tag="ACSIGJ_000358"
     CDS             complement(54071..54430)
                     /locus_tag="ACSIGJ_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HHQ1329331.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            complement(54435..>54959)
                     /locus_tag="ACSIGJ_000359"
     CDS             complement(54435..>54959)
                     /locus_tag="ACSIGJ_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HHQ1329332.1"
                     /translation="AGTVWGGKKVELNPAKWDWVKNTGYKKPAARHMQTVDGEMAGGN
                     RPPKSITSEGKANAATYPKLVNQLNEQNLNNIAAQDPRLSLAIHEGKKNFPIGTATYE
                     EADRLGKIWVGEGARQTSGGGWLSRDGTRQYRPPTEKKSQFATTGIQANFETYTIDSN
                     EKRNKIKNGHLNIR"
BASE COUNT        12963 a        12970 c        15302 g        13724 t
ORIGIN      
        1 gcggaaaacc tgccttttca aagttttatt catattttat tccttaagtt tgccttcgcg
       61 ggacggcgcg ccgcgaacgc cggggttcgg caaaccgccc gattccgcgc ccgccgaatt
      121 gctgattgaa aagcttaccc cccattttaa ctttgcgcac ttatcacggt aaagcgtttt
      181 ttagcaaacc gccgcagatg cccgaccggt ccggattccc gcctgcgcgg gaatgacggc
      241 atcggtttga cggtatttaa ttgaattgcg gaaattgacg gattcagtga gattggcggg
      301 atgaagccta ccctatagcc cgccttttat agcggattaa caaaaaccgg tacggcgttg
      361 ccccgccccg gctcaaaggg aacggttccc tgaaggcgcc caagcaccgg gcgaaccggt
      421 tccgtaccat tcgtactgcc cgcggcccgc cgccttgtcc tgatctttgt taatccgcta
      481 tgcttataca caaccagcca aaagccatct tattgaaata tcataagatt tactcaaaaa
      541 tcttattttc tccaccactt atgcacaaac tcggatacac actctgccaa tatgcagaaa
      601 aaaagacatt cgcaccgaca ccttttcatc cccacaagtt ccaaacacat ttccggctta
      661 aatatccaca gcattcccgt tctcaaaggc accggcagcc attcggtaaa gaaaagcggg
      721 atgtcgtccg cacaacaagc aaactatcca caaaacttat tccccacgaa aaacaatcca
      781 caggtatgca caaaagggaa tcctccttac cgacaccctt ttaaaacggc aattcagatg
      841 attttaaaaa taaaaaaaag ttacccacag aaaattcccc atatcaatat aatcatcgtt
      901 ttattattta aaccttatat tttatttata acccttcagg gaaacggcac ggcaaagcgt
      961 aaaggcatct tttcaaaaaa ttaaaaagtt aaggattgtt aaacaagtgt aaatcaggta
     1021 gagttgcagc cgataaatta aatctataac ggagcttcct gcaatgaaag gcgatattgg
     1081 cgtaatcggt ttggcggtta tgggtcaaaa cctgattttg aatatgaacg attgcggatt
     1141 taaggttgtc gcctacaacc ggacaatcgg taaagtggac gaatttttaa acggtgcggc
     1201 aaaagagacg ggcattatcg gcgcgtattc cctgcaagac ttggtcgata agctggcaaa
     1261 accgagaaaa atcatgatga tggttcgcgc aggttcggtg gttgacgact ttgtcgaaca
     1321 actgcttccg ctgttggaag aaggcgacat cattatcgac ggcggcaatg ccaattaccc
     1381 cgacacgaca cggcggacac attaccttgc cggaaaaggg attttgtttg tcggcgcggg
     1441 cgtatccggc ggcgaagaag gcgcgcggcg cgggccgtcc atcatgccgg gcggggataa
     1501 gcgcgcttgg gaagcggtca agccgatttt ccaggcgatt gccgccaaaa cgccgcaggg
     1561 cgaaccgtgt tgcgactggg tcggcaagga tggcgcggga cattttgtca aaatggtgca
     1621 caacggcatc gaatacggcg atatgcagct gatttgcgaa gcgtaccagt ttatgaaaga
     1681 cggtttgggg ctgtcctacg acgaaatgca ccgcgtgttt gccgagtgga acaaaaccga
     1741 gctggattct tatctgattg aaatcacagc ggcaattttg ggctataagg acgaaggcgg
     1801 cgaaccgctg gccgagaaaa tcctcgatac ggcagggcaa aaaggcacgg gcaaatggac
     1861 gggcatcaat gccttggatt tgggcattcc gctgacgctg atttccgaag ccgtattcgc
     1921 ccgctgtgtt tcgtcgttca aagaacagcg cgtgcagacc ggcaagctgt tcgcccgaac
     1981 cgccacgccc gtcgaaggcg gcaaacaaga atgggtcgag gcgttgagac aggctttgct
     2041 tgcctccaaa atcatttcct acgcacaagg ctttatgttg atccgcgaag cgggcgaaag
     2101 ttacggctgg ggtttggact acggcaacac tgcgctgctg tggcgcgaag gctgcatcat
     2161 ccgcagcgcg tttttgggca atatccgcga cgcgtatgag gccaatcccg atttggtgtt
     2221 cttgggtgcg gatccgtatt tcaaaaatat tttggaaaac tgcctgcctg catggcgcaa
     2281 ggtggttgcc aaggcggtcg aatgcggcat ccctatgccc tgcatggctt cggcgattac
     2341 gttcttggac ggctatacga ccgaacgcct gcctgccaac ctcttgcagg cacagcgcga
     2401 ctacttcggc gcgcacactt acgagcgcac cgacaaaccg cgcggcgaat ttttccatac
     2461 caactggacg ggcaagggcg gagataccgc ttcgacaacc tacgatattt gattccgttg
     2521 ccgaaatgcc gtccgaaccg ttcggacggc atttgttttg caggaaatag aaagatgttc
     2581 caatggcttt atgatgtatt gtggctgctt gcgccgatat ggatacggcg ttatttggac
     2641 aaacgctccg gaagtgcccc ggcataccgg gcgcataggg acgagcgttt cggcaagccg
     2701 catccgaatc ccgttaccgg cgcggtttgg atacacgccg tttcggtcgg agaaacgcgt
     2761 gccgcccagc ccttgatacg cgagttgcgg cggcgttttc ccgatgcgcc gctgctgatg
     2821 acgcagatga ccccgacggg acgggaaacc gcgcaagttc tgtttcccga tgcgcaatgc
     2881 cgctatcttc cgtatgacaa aaaaacgtgg gtacggcagt ttttgcgcga acaccgcccg
     2941 atgttcggca ttttgatgga aaccgaaatc tggcccaatc tgatgaagga atgccggcga
     3001 gcgggtgtgc cgctgttttt ggcgaatgcg cggctgtcgg aaaaatcgct gaacggttat
     3061 ctgaaagtcc gccgcctgat ccgtcccgca gccgcttcgc tgacgggctg tctggcgcag
     3121 acggaggcgg atgcggcgcg gttggcgaaa ttgggcgcgg catccgtgca ggtgtgcggc
     3181 aataccaaat acgacctgat gccgtctgaa gacatgaaaa cgctggcggg gcagtttgaa
     3241 aaacgcatcg gaggccggcc ggttgccgtg tgcggcagca cgcgcgttta tcggggcgaa
     3301 gacgaggcgg aaaaactgct ggcggcgtgg caacaatatc gcggcgatgc cctgctggcc
     3361 gtcgtgccgc gccatcccga gcattttcag acgacatttg aaacggcgaa acgcttcggg
     3421 tttaaggttc agcggcgcag cgacggtttg ccggtcgaac ccgatacgca ggtgtggata
     3481 ggcgacagta tgggcgagct gtatgcgtat tacctgtgcg ccgatgtcgc ttttgtcggc
     3541 ggcagtctgg tcggttcggg ctgtcagaac atcatcgaac cgctttcctg cggcgttacg
     3601 acgatattcg gcttttcgac ctacaatttt tccgaagcct gccggcacgc cttggcatcg
     3661 ggtgcggcgg ttcaagtcga atcggcggac gcgtggcgtg aagccgttga aaaaaactta
     3721 tctggcgagg ggggcggaat gcagatgcag gcgcgcgtgg acggctttat cgcacaacat
     3781 cgcggagcgg gcgcgagaat cgccgaggcg gtgcgggaag cggtatgcgg acatcggggg
     3841 cgatagtgat acaatccgta tccgagtttt ccggttggag taccgtatga gtatttatgc
     3901 cgtcgcgcac atcatccacc tgtattgcgc caccgccttt gtcggcggcg tgttttttga
     3961 agtgctggtt ttgtccgtcc tgcatacggg acgggtgtcg cgcgaggcgc ggcgcgaagt
     4021 ggaaaaggca atgtcttacc gcgccgtcag ggtgatgccg tttgcggtcg gactgctgtt
     4081 cgccagcggc atcgtgatgg cggcaaaccg ctaccttcct atatcgggcg aaccgtttgc
     4141 cacttccttc ggtacgatgc tgacgctgaa aatcctgttg gcgttcagcg tgttggcgca
     4201 cttcgccgtc gccgtcgtca aaatggcgcg ttccacgctg acggtcggct ggtcgaaata
     4261 catacacgcc gtcgtcttta cccatatgct gctgattgtc tttttggcaa aagcgatgtt
     4321 ttatatcagc tggtaacaca actacatttc aaatcaggag attgaaccat gacagaatat
     4381 cccggcatcg ggtcgccgtt gttttacggc gttttttttg cggcagtgct ggtcatgatt
     4441 gccttggata tgttttcgct gaagaaaaac ggcagccata aagtcggcat caaagaaacc
     4501 ttggcatgga gcggcttgtg ggttgccgta tcctgcctgt tcgcaggctg gctgtatttc
     4561 gaactcgccg gcaatcccgg ctacggcgcg gcagccgcaa aagaaaaagt attggaattc
     4621 tttaccggct acattttgga aaaatcgttg gcagtcgaca atattttcgt gttcctgatg
     4681 atattcggct acttcaaagt cgcaccgcag tttcaacacc gcgtgctgct gtacggcgta
     4741 ttgggcgcat tggtattgcg caccgtcatg attttcgtcg gcgcggcact ggttcggcag
     4801 ttcgagtgga ttctgtatct gttcggcgcg ttcctgctct ataccggcat acacatgatg
     4861 aagcccgaag gagatgagga agaggatttg gcaaacagca ggctgctgaa tgccgtcaag
     4921 aaagtcgttc cggtcggcac ggcgtttcac ggcgagaaat tttttaccgt cgagaatggc
     4981 aaaaaaatcg ccacgccgct gtttttggtg ctgattatga ttgaattgag cgatgtcgtt
     5041 tttgccgtgg acagtatccc cgccgtcttt gccgttacca ccgatccgtt tatcgtgctg
     5101 acttccaata ttttcgctat tttgggtttg cgggcgatgt atttcctgct ggcggatgtg
     5161 gcggaacgct ttatcttcct gaaatacggc ttggcattcg tgttgggttt tatcggtgtg
     5221 aaaatgctgg tgatgcattg ggtacacatc ccgatttccg tttcgctgtc ggtcgtgttc
     5281 ggcgcgttgg gtgcatcgat actgacctcg ttaatttata cgaaaaaaca gcctgataaa
     5341 taaaacgccg tctgaacgcc gacaaaatgg ttgacagcat caggcttgat gtataaactt
     5401 tcaaattttt cgggtcgtta gctcagtcgg tagagcagcg gacttttaat ccgttggtcg
     5461 agcgttcgaa tcgctcacga cccaccaggt aaagcaaaaa agccgagttt tcaaaactcg
     5521 gctttttttt gttttccgac aatccttgtt cggaaagcgc ggcatttcgt caaattcttt
     5581 ggtgcggctt ccgtattttt cccgaaaatc ccttataatt ccgcctctgc aaccttcatc
     5641 gggtgatgct ttatgcattg tcccgagtat tgaaatatta gcaagtaccc accaggaggg
     5701 aacaaacgtg gacaaactga aaatctccgc aaacggcccg ctcaacgggg aaataacggt
     5761 ctcgggcgcg aaaaacgcgg cattgccgct gatgtgcgcg ggtttgctga cgtcgggtac
     5821 gttgcgcctg aaaaacgtcc ctatgctggc agatgtcgca accacgcaaa agctgcttca
     5881 gggcatgggc gcgcgcgttc tgaccgacaa tatcagcgaa tttgaaatca acggcggtac
     5941 ggtcaacaat acctgcgccc cttacgaatt ggttcggacg atgcgcgctt cgattttggt
     6001 gcttggcccg acgctggcgc gtttcggcga ggcgcaagtc agcctgccgg gcggctgcgc
     6061 catcggttcg cgccccgtca atcagcattt gaaaggcttg gaagcgatgg gggcggaaat
     6121 cgccatcgaa cacggttacg tcaaagccaa aggcaaactc aaaggtgcgc gcgtggcgat
     6181 ggatgtcgtt accgtcggcg gtacggaaaa cctgctgatg gcggcgacgc tggcggaagg
     6241 tacgacggtt ttggaaaact gcgccatcga gcctgaagtg gtcgatttgg cggaatgcct
     6301 ggtcaaaatg ggcgcgaaaa tcagcggcat cggaacatcg accatgattg tggaaggtgc
     6361 gggcgagctg tacggctgcg aacacagcgt cgtccccgac cggattgaag cggggacgtt
     6421 cctgtgtgcg gtggcgataa ccggcggcag ggtggttttg cggaatgccg ccccgaaaac
     6481 gatggaagtg gtgttggaca aactggtcga ggcaggtgcg gtgattgaag cgggcgacga
     6541 ttggatcgcc atcgatatgc ggcagcgtcc gaaggcggtg gacatccgca cggtcgtcca
     6601 tcccggcttc cccacggata tgcaggcgca gtttatggca ttgaatgctg tggcggaagg
     6661 aagctgccgc gtggtggaaa cgatttttga aaaccgcttt atgcacgttc cggagttgaa
     6721 ccggatgggg gcgaacatta caaccgaggg caatacggca tttgtgcagg gtgtggaacg
     6781 gctttccggc gcggtcgtca aggcgacgga tttgcgcgcg tccgccagcc tcgttatcgc
     6841 cggtttggcg gcgcgcggcg aaaccgtggt cgaacggatt taccacttgg atcgcggtta
     6901 tgaaaatatt gaaaaaaaac tcggcagcgt cggtgcgaac atcgagcgcg tatccggctg
     6961 atcgggttgc gatgccgtct gaaggcttca ggcggcatca attgtttctg tatcgcaaac
     7021 actgattgaa aaggactgat tatggcattt ttaaaactga ccgaacaaaa cgtgcggggc
     7081 aaaaccgtcc tcatccgcgc cgatatgaac gtgccgttca aaggcggcaa aatcagcgac
     7141 gacacccgta tccgcgcctc gctcgcgtcc gtcaaatact gcttggacaa cggcgcgtcc
     7201 gttattgtga tgacccactt gggccgcccg accgaaggcg agttccaccc cgaagacgac
     7261 gttgcgcccg ttgccgcgca cttgggcggc ctgttgggca aagacgtgaa agtattgaac
     7321 gactggcgcg aaaacaaacc ggctttgaac gcgggcgatg tcgtgatgct gcaaaacgtg
     7381 cgtatcaaca aaggcgagaa gaaaaacgat ttagaactgg gcaaagccta cgccgccttg
     7441 tgcgacgtgt tcgtcaatga tgcgttcggc accgcacacc gcgcccaagc ttcgaccgaa
     7501 gccgttgccc aagccgcgcc cgtcgcctgc gccggcgtat tgatggcggg cgaactcgac
     7561 gctttgggca aagccttgaa acagcccgcg cgcccgatgg tggcgattgt tgccggcagc
     7621 aaagtgtcca ccaagctgac catcctcgaa tcgttggcgg acaaagtcga tcagcttatc
     7681 gtcggcggcg gcatcgccaa cacattcctg ttggcggaag gcaaagccat cggtaaatcg
     7741 ctggcggaac atgatttggt ggaagaatcc aaaaaaatta tggcgaaaat ggcggccaaa
     7801 ggcggttctg tgccgctgcc gaccgatgtc gtcgttgcca aagcctttgc cgccgatgcc
     7861 gaagcggtgg taaaagacat cgccgacgtt gccgaagacg agatgatttt ggacatcggc
     7921 ccgaaatccg ccgccgcgct tgccgagttg cttaaagccg ccggtacggt cgtttggaac
     7981 gggccggtcg gcgtgttcga gttcgatcaa ttcgcaggcg gcacaaaagc ccttgccgaa
     8041 gccatcgccc aaagcaaagc gttctcgatt gcgggcggcg gcgacacgct ggcggcgatt
     8101 gccaaattcg gcgttaccga acaaatcggc tacatctcca ccggcggcgg cgcgttcctt
     8161 gagttcttgg aaggcaaaga gctgcctgcc gtagccgctt tggaaaaaac gcggcgagta
     8221 aacggtttga tataaaaaaa tgccgtctga aacgaaatgg tttcagacgg catttttgtg
     8281 tggcggttat tgtgctttgg cagcccattc cgccggagtg gcggcaaccg aaatggaaag
     8341 tgcgtgcagt tcgttgcttt ccagttgggc tttgagtccg tctttaatca ggcggtggcg
     8401 cgcgaggcgt gccttgcctt caaattctga tgaaacgatg acggcgaaaa aatggtgtcc
     8461 gtcgccttct acttcgatgt gttcgcattt tgccacgcct gcaatcaggg ccttgacttg
     8521 ttcgggtgtc agcatagtgg ggagtcctta ttggggaaat gcgccattat actggcaaat
     8581 cgaaattccg tctgaaaacg gcttcagacg gaatttttga cggtgtttta ttttttggct
     8641 ttgaagcaga gcaggtgttg tgtggtggca agccacgcgc cgaatacgcc caacgcgatg
     8701 acgaagccga acaccagccc gagttcgccg acgtagaaga aacgccagcc gatgttcaag
     8761 ccgtagggtt tgaagatggc atcgaccaag gggtgcacgg cagagagcag ccaaccgcaa
     8821 agccctaagc tgacggcggc ggagaagata ctctgccaca tagcctgata aaggaagggg
     8881 cggcggataa acgacgcggg cgcacccagg agtttggtga tttcgatttc ttctttgcgg
     8941 ctgaggattt gcagtcggat ggtgttgtgt gcgacaagga cgaaagccat acccagcgtc
     9001 agggaaagaa accataagat tttgcggatg aactcgttga tttggtacag cgtttgcacc
     9061 cattcggtat ccatagacgc ggattcgacc ataggcagct tggtaatgtc ccggtagatt
     9121 gcctgcattt gggcgggcgt ggttgccggg tcgggggtaa cgataaagac atccggcagg
     9181 gggttgccgt caagcatgga aatcagattt tggtcgaggt tggactgtaa ttccgccaaa
     9241 ccgtcttcct tgccgatgaa gcggatgttg tcgagccgtt tatcgcgcgt cagcaggctg
     9301 cgtacggtat cgctgtcgct ttgtgcggcg gcggtttcca tatagacggt gatttgcggc
     9361 gactcgttga gtttgcccaa cacgctttgc ccgctttgga tgcccagata cataaacagc
     9421 ggcagggtca tcgcgacggc aagcattatg agggtaagca gtgtgccgaa gggttggcgc
     9481 agaagctgtt tgagtgcgga gcgcgcggat tcgacgtgca gcgagaagta gtggatgatg
     9541 ctcatgcgag tcgtcctttc gagaggcgca ggatgcggtg tccgtagtcc gccatcaggg
     9601 tttcgtcatg tgcggcgacg atgacggtgg ttcccgcttc gtggaaggtt ttgaacaatt
     9661 ccataatatc gagcgcgtag gcacggtcga ggttggcgga gggttcgtcg gcaatcagca
     9721 ggccgggctg gtggacgacg gcgcgggcga tgcacaggcg ttgttgttca ccgcccgaga
     9781 gggttacggg atcgtccaat tctcgtcctt tcaagccgac tttttcgatg gcgatgcggg
     9841 cgcgttcttc ggctttgcgc ggcggatagc cgataatccg caggggcagg atgacgtttt
     9901 gcaggacatt gcggtcgttg aggattttgt ggtcttggaa cacgatgccg atgtgttggc
     9961 gcataaagcc gatttggttg tcggacaatg tgccgaggtc ctgcccgtta aacaggactt
    10021 tgcccatgct cggcttggtg atgcccgaaa tcagcttgag gacggtggat ttgcccgaac
    10081 cggagtgtcc cgcgataaaa atcatctcgc ctttgttgat ttggaagctg acgtttttca
    10141 gggcttcaaa accgccggga taggttttgg aaacttgttc gaaacggatc ataggaggtc
    10201 ctgtaaggaa gggcggcata tcgtttcggg cggttgcgtc cgcagggata tgccgtctga
    10261 acaataggta agacagtacc gattataaag attccgcctg tttttagata gtggataagt
    10321 ttggcggata ccgaaggccg ggcgggaaaa atgccgtctg aagcggcttc agacggcatc
    10381 cggcgtttaa cggcattttg aatgggcgag tttgacggct tcggtcaggc ttttctcgtt
    10441 cacctctcct gtaatggtct gcctgtatcc gcatttcggg gcttcgacga cggtaaaggg
    10501 cagtacgccg acattgtttc cgtaggattt cataaagttt cggctgttcg ccccggtgta
    10561 acgccaaatc gggtagctga ccggggtctg cttgagaaag ttgccgatat tgtcggatgt
    10621 gtcgagcgcg atgccgacca tatcgacgct gccttttttc tgcgctttgt accatttgga
    10681 catggcgggc atctcttttc gacacggacc gcaccaagtc gcccataggt tggcgatgcg
    10741 gacgggggct ttgagcgatt gcaggttttg cggggtattg tctttccatc cggccagttc
    10801 gtctgccgag gcggtatgtg cggcaaatgt ggcggcaagg gcgattgcgg cgagattcaa
    10861 gcgtttcatc gttttgtcct ttgtatgtcg attcgggtat tgtaaaccgt ttggtacggt
    10921 tggaggcggg gtattgagta aaaccctgcg taagccgata ataaggggat tgtcgagaag
    10981 tagggaaatg tcatgtctga aatcaaaatt ttccacaatc cgcgttgcag caaatcgcgt
    11041 gccgccgtgt ccctattgga agaacgcggc attgctgccg aagcggtcaa atatttggat
    11101 acgccgcccg acttgtccga attgaaggat attttcaaca aattgggctt ggaatcggcg
    11161 cgcgggatga tgcgcgtgaa agatgatttg tacaaggaat tgggtttgga caaccccgat
    11221 ttggacaatg acgcgctgct gcgtgccatc gccgatcatc ccgccctgtt ggagcgtccg
    11281 attgttttgg caaacggtaa ggcggctgtc ggcagaccgt tggaaaatat cgaagcggta
    11341 ttgtgaacaa acggctgttt tgcagccgca acggcttgcg ctattacctg ttgggcggtt
    11401 tctgtttgag tgtttttccg cttttgctgg tgtttgcctc atccgtttgg gcggtttatc
    11461 ggacgggcgg gcaggttttg ccgccgtatg tccgggcgga tgccgcgctg gtgttgggcg
    11521 cagccgcgtg ggacaaacgc ccttccccgg tttttcggga acgcatcaac cacgccatcg
    11581 cgctttacca aagccgcagg gtaggcaaaa tcgtgtttac gggagggcga accaaaaaag
    11641 gctatatgac cgaagccgag gtcgggcggc ggtacgcgct caaacagggg gttccggcgc
    11701 gcaacatcct gtttgaaaac acttcgcgca acacttatga aaacctgaac aacatccgtc
    11761 cggttttgcg tgccaacggt attgccagcg tggtgattgt cagcgatccc taccatttgg
    11821 cgcgggcggc ggaaatggcg gaggatttgg gtgtccgcgt ttatatgtct gccacgccga
    11881 ccacgcggtt tgatgcggga aataagaaaa aaatctttat gctgcaagag gggtatgcgt
    11941 tatccctcta ccgtttggaa aaatggggca gccgcttttt gggatggctg tctgattgaa
    12001 gcggtagaaa atgccgtctg aaacttatcc gcaggcaggg ttcggacggc atttttgatg
    12061 cctaagcacg gtaaaaacag taaaataaac gctttcaaag atttcggagc cattatgacc
    12121 gtcaaaaccc gtttcgcccc cagccccacc ggttacctgc acatcggcgg cgttcgcacc
    12181 gccttgtttt cgtgggcgtt tgcccgccat cataaaggcg aatttttatt gcgtatcgaa
    12241 gacaccgact tggcacgttc caccgccgaa tccgtcaaca tcatccttga cggcatgaaa
    12301 tgggtcggtc tcgattacga caacgccgac aacgtggtgt accaaacccg ccgtttcgac
    12361 cgttataaag aagtcattgc cgaactcttg gcaaaaggcg atgcctacta ctgctattgc
    12421 agcaaagaag aattggaagc gatgcgcgag aaagccgaaa aagaaggcac ggcgacttac
    12481 gaccgccgct ggcggccgga agcaggcaaa accctgcccg aaattcctgc aggcgtgcaa
    12541 cccgtcgtcc gcttcaaaac gcctttggac ggcgttacca aatggacaga cttggtcaaa
    12601 ggcgaaatct ccatccccaa cgaagcccta gacgacctga ttattgcccg cgccgacggt
    12661 acgccgacct acaacttctg cgccgtggta gacgactacg acatgggcgt tacccacatt
    12721 atccgcgggg acgaccatgt gaacaacacc ccgaaacaaa tcaacatctt gaaagccatc
    12781 ggcgcgaacc tgcccgaata cgggcatctg ccgatgattc tcaacgaaca aggcaaaaaa
    12841 atctccaaac gcagcggcga taccgtcgcc attaccgatt tcggcgcaat gggcattctg
    12901 cccgaagcga tgctcaacta tttggcgcgt ttgggctggg cgcacggcga cgatgagttt
    12961 ttcacgacgg aacaattcat cgaatggttt gatttgaaag acgtttcccc gtccccaagc
    13021 cgtatggatt tgaaaaagct ctattggatc aacggcgaac acatcaaaat cacacccgac
    13081 ggcaaactta ccgaactcgt caaaccgcgc cttgcgctgc gcgatattca tgaaaccgaa
    13141 aaacctgcgt tggaagacgt gttggcactg gtcaaagacc gcgcccaaga tttgaacgcc
    13201 ttggcagacg aatgcctgta tttctacaaa aaacaagtcc ctgccgaagc cgatgttgcc
    13261 aaacactggg acgacgaagc cgccgcccgt atgctgcgct tcgccgaacg cctcgaaggg
    13321 ctggaagact ggaacgccaa agccattcac gacctcttca aacctttctg cgacgaagaa
    13381 ggcatcaaaa tgggcaaact cggtatgccc ctgcgccttg ctgtctgcgg tacggcaaaa
    13441 accccgagcg tcgatgccgt gttggcatta atcagcaaag aggaagtgtt gaaacgcatc
    13501 cgtgcttaag ttatccacag gtgcgggcaa tgccgtctga tgtcggaatg cggcttcaga
    13561 cggcattcag acggaagttg aacatgaaaa aaatattgta ttttctgatg tttgtttttt
    13621 ctacaagcgt atgggcaggg ggtgcggaag acaatttgct cagcatccaa tccggttacc
    13681 gtgctttact gcaaaagcaa aacaatctgg acggaaaaat catcgggatg cagtcggatt
    13741 tggaagatgc gcgccggcgt ttgcaggcgg ctcaggcaga catcgcccgt ttggaagcgg
    13801 aaattcctgc agcaatggcc caaaaagccc ggcaggctga agagatgagg caaatcggag
    13861 tgcgtttgga ccatgcttgg aatgcggttt acggcgcagg ggaacgaaag cgtcggggga
    13921 attgaccgat gaattccctc tttgtggaca acactgtttt tattacacgg ctgaaagccg
    13981 ggcatatcgg caggttggtt caggcgttgt ttgaggagtg gcacggattt gaaccgtggt
    14041 cttctgtgga taagattcgt gcctattacg gcagatgttt gaaggatgac gaattgccgc
    14101 tggcttttgc ggctgtggat gattccggaa ccctgttggg ttcggctgca gtcaagcggc
    14161 atgatatgga aaattttcca cagtatgaat attggttggg cgatgtcttt gttttacctg
    14221 aatatcgcgg aaaaggcatt ggcaggaggc tggtcgccca ctgcataggc gcagcgcgtt
    14281 cgctggggat aaagttcttg tatctttata cgcctgatgt gcaaatattt tatgaatcat
    14341 tcggctgggc ggttgtcggg cgacatttcc ataacgggga atgggttacg gttatgcgtt
    14401 tggatgtgga taaggtttaa gccgaatggg cagatgccgt ctgagctgtt cggacggcat
    14461 tttttatgtt tggaaatttc ctgtcagata tagtggatta acaaaaaccg gtacggcgtt
    14521 gccccgcccc ggctcaaagg gaacgattcc ctaaggtgcc caagcaccgg gcggatcggt
    14581 tccgtaccat ttgtactgcc tgcggcccgc cgccttgtcc tgatttttgt taatccgcca
    14641 taaagtttat ccacagattt ttccatatgg tttgatgctg ctccgccaac ggaaattaag
    14701 atacggattt gcacaaatct ttcagacggt acagttctga caatgcttcg cgcggggtca
    14761 ggctgtcggg atcgaggttt tccagggctg ctgccaatat accttcaaaa tgtttttcct
    14821 ctgctttatc cacaaagcag tccacattcg gttcatctcc tttttcagac ggcatggtac
    14881 tgaaaatatc cagttgggga cggtttgcgg cggcttggtt ttccagtccg ttcaaatgct
    14941 tttgggcggc tttcaatgcg cgcacaggca ggccggcgag tttggcgacg gcgatgccgt
    15001 agctttttcc ggcgggaccg ggttggattt ggtgcaggaa cacgatgtcc cgtccctgtt
    15061 cgagcgcgga aaggtgcata ttgacggcgg cggcgtgggc ttcgggcagg taggtcagct
    15121 cgaaatagtg ggtggcaaac aggctgaagg atttgttttt ttgcagcagg tgttcggcga
    15181 ttgcgtgcgc gagggcgagg ccgtcgaaag tggaagtacc acgtccgact tcgtccatta
    15241 aaacaatgct ttgttcggtg gcgtgatgca ggatgtaggc ggtttcgctc atttcgacca
    15301 tgaaggtgga gcggttggag gcgaggtcgt ccgatgcgcc gatgcgggtg aagatttgat
    15361 cgacaggccc gattgtggcg gcatcggcag gcacgaaaca gccggtgtgt gccaataaaa
    15421 caatcagcgc gacttggcgc atataggtgg atttgccgcc catattgggg ccggtgagca
    15481 gcatgaggcg gtgtttgtgg tcgaggtcgg tgtggttggc tgtgaagtgg cgtacctgct
    15541 gttcgacaac gggatggcgg ccgttttcga tgtggacaac cggatagtcg gcaaactcgg
    15601 gacggacaaa gttccgctct tttgccaagg ctgaaaatgt ggacaacacg tccagcgcgg
    15661 cggcggcttt ggcggctttt tgaagctgcg gcaatgccgt ccgaaggttt ttcaatacgc
    15721 cgtcaaagag ttgtttttct aaggcgaggg cttggtcttg agcagtcagc actttgtctt
    15781 caaaggcttt cagttccggc gtgatgaagc gttcggcgtt tttgagggtt tgccggcgtt
    15841 ggtagtcggc aggtgcttgt tcggcttggg ttttggacaa ttcaatgtaa aagccgtgaa
    15901 cgcggttgaa ctcgactttg agtgtggaca aaccggtacg ttcgcgttcc ttggcttcca
    15961 aatccagcaa aaattcgtcg ccatggtttt gaatgcggcg caattcgtcc agttcgggat
    16021 gaaaaccgtg gttgatgaca ttgccgtctt tcagccagac ggaaggttcg ggcatcacgg
    16081 cggctttgag ggtttcggca actggcaggg tttcggggaa aacggcttta agggtttcca
    16141 acaggctgct gccggttgcg gacaagtcga tttgtgccaa ctcgaacaga ctgtcgcgta
    16201 aagatgccaa atcgcgcggg cgggcattgc cgacggcgat gcgggcggcg atacgttcga
    16261 tgtcggcaat gctcttcaga tggcattgga ggggttcgta ttggctttcc agcgcggtta
    16321 cggcttcttg gcgcgctcgg atgtgggcgc ggttgcgtaa agggtggtgc agccacaatg
    16381 ccaacaggcg gctgcccatg tgggtggcgc agccgtcgag tatggaaaac agggtcggcg
    16441 tttttttgcc ggagagggtt tgcgtgattt cgagattgcg gcgcgtggcg gcatccatgc
    16501 cgatatattg gctgtcggtt tcgagcgaca ggccgtccaa atgttgcggc atcaggtttt
    16561 gcgtcaggcg gatatagttc aacagcgcac ctgccgcgcc aatcgaaacg gcgtgttctt
    16621 tgctgtccaa accgaagccg cgcaaatctt ggcagccgaa atattccgtc agcagttttt
    16681 cccccgcgtc ggcggcaaac tgccacgcgt tcaggcgtgt aacacccgat gccgtctgaa
    16741 gttgcggtgc gtttttactg tccggcagca aaatttccgc cgcctgcagg cgcgccagtt
    16801 cgtcgttcaa tttatccgca gttgtcagct tggttttgaa ttcgccgctt tgcagcgatg
    16861 cccacgccaa accgatgtat tttttgtcgg gagacacggc aacgatgcgg ttggtttcct
    16921 tgtcttccag taatgcggaa tcggttagcg tgccgggcgt tacgatgcgc acgactttgc
    16981 gctccaccgg ccctttgccc gcgccgactt cgccgacctg ttcgcaaatc gccacgcttt
    17041 tgcccaactt gaccaggcgc gccagatact gttccgccgc gtgaaacgga acgcccgcca
    17101 ttttaatcgg cacgccgtcc atctgcccgc gtgtggtcag ggtgatgtcc aacaatttcg
    17161 ccgcttccac cgcgtcatcc aaaaacagct cgtaaaaatc acccatacgg taaaacacta
    17221 gtttgtcggt atgttgcgct tttatgccga ggtattgctg catcattggg gaaacggcgg
    17281 atttggacat ggggctgcct tattattagg aaaacggtat tgtagcaaaa ggttttgtct
    17341 tcgacggaat gagactgacc gtaagcttgt caataaagtt gtggagacaa ataggaaaac
    17401 ggcaaagtta tccataagaa tattattaag tcaaaaaaca tccacgctta tctgtcttat
    17461 acggcaagcc ttaaactcaa atttatttaa caaaaaggta atgaaaatat tggatatttg
    17521 ctacttatac acaagcaggt cggcaatcat caaaatcatc attaaaattt taaaatcaaa
    17581 tattgaatgc gcgtttttga aatagcgaaa ttcacctgcg tcaaacaaaa cgcaagtaat
    17641 tttatgtaat agtggaatgt agttttattt cagaatacaa tacaggcatc ggactttatt
    17701 tcacaaccgg gagacaaaat atgagcatca aagtagcgat taacggtttc ggccgcatcg
    17761 gccgcctcgc attgcgtcaa attgaaaaag cccacggcat cgaagtcgcc gccgtcaacg
    17821 acctgacccc tgccgaaatg ctgctgcacc ttttcaaata cgacagcaca caaggccgtt
    17881 tccaaggtac tgccgaatta aaagacgatg ccatcgtggt aaacggcaga gaaatcaaag
    17941 ttttcgccaa tccgaatccc gaagaattgc cttggggcga gttgggtgtg gatgtcgtcc
    18001 tcgaatgtac cggtttcttt accaacaaaa ccaaagccga agcacatatc cgtgccggcg
    18061 cgcgcaaagt cgtgatttcc gcacccggcg gcaatgatgt gaaaaccgtc gtatatggcg
    18121 taaaccaaga catcttggac ggtagcgaaa ctgtcatttc cgccgcttcc tgtaccacca
    18181 actgccttgc cccgatggcg gcagtcctgc aaaaagagtt cggcgtagtc gaaggcctga
    18241 tgaccaccat ccacgcctac accggcgacc aaaacaccct tgacgcgccg caccgcaaag
    18301 gcgatttgcg ccgcgcccgc gccgccgcgc tcaacatcgt gcccaacagc accggcgccg
    18361 ccaaagccat cggtctggtt atccccgaat tgaacggcaa actcgacggc tccgcccaac
    18421 gcgtccctgt tgccaccggc tcgctgaccg aattggtttc cgtcctcgaa cgccctgcaa
    18481 ccaaagaaga aatcaacgcc gcgatgaaag ccgcctccag cgaatcttac ggctacaacg
    18541 aagatcaaat cgtttcttcc gacgttgtcg gcatcgaata cggctcgctc tttgacgcaa
    18601 cccaaacccg cgtgatgacc gtgggcggca aacaactggt gaaaaccgtt gcctggtacg
    18661 acaacgaaat gtcctacacc tgccaactcg tccgtacttt ggaatacttc gcaggcaaaa
    18721 tctaaggctt gaacaaacct gtggataagt aaatgccgtc tgaacacatt gcgttcagac
    18781 ggcattgttt atctgtaact tgatattgcg gcttatggcg aacaaacaac agccgtttgt
    18841 tcaactgcta tgcggcaaaa ccggattttg cccgatttaa agcggggttg gggaaagatt
    18901 aataaccgtc gcgcggtttt cctcgatttg aaacctgact tcgtaatccg caatattcat
    18961 cacataaatc cgttcgggaa tattctgata ggcggggcgc ggatcttggg caatgctttg
    19021 gctgataagg tttttggtgt ttgcagataa attttccgcg ccgatgtttt cctgccaaac
    19081 gacttccaac tctacgggtt tgccgctgac gaaaccggat gcggcatcgg gtttggattc
    19141 gataaagggg atataaggct tgatgtccac aatcggcgtg ccgtccagca ggtctgagcc
    19201 gctgcaatag aggcggacgg gtttgccggt ttcgatgcgt tcgagtttaa ggagagagag
    19261 tccgagatgg ttggggcggt gggggctgcg cgtggcgaac acgcccattt tttgtttgcc
    19321 gccgagccgt ggcgggcgca ccatttgcgc ccaaccttca tccaatacgc cgtggaagat
    19381 aaaacttatc cacacataat cgaaatcttc caacccgcgc acgctgtctg cggtaaattc
    19441 gggattcagc tcgatgcagg cttctgcggc ggagaccaaa ccgggctggc gggcgatgcc
    19501 gaatttctgt ttgtagggcg agcgggcggc gccgatgggg gtaatggtat aagtcataag
    19561 attgtggata agttttttgg aaaaacctta tcttatcaca gccggtttta ccgctttcgg
    19621 gtagtaattt gttataatac agggcattta aatcgcgttg aaataaagta gcacagaatc
    19681 ctcgtgtcca agacgctctt aaaaaggcat tggagtattg ataaaaaaga agcataacgg
    19741 ttcgttataa cgaagaggga atcccgtata ttacacccaa gtcaggttgt tatctttcct
    19801 tttggatctc acttacagga ggtgagatct gaaattttgg gtaataaaat cggattacag
    19861 cttctagcaa ggagtacctt aggtatagct aaaatggagg gggtggatat gacatatcat
    19921 atccacctag atggattgta ctccttaaat aaagaaggta agagttttgt tattcgtaaa
    19981 atagagaatg acgatcttaa atgaagaagt aatgagatga ttgtttccat tgatgttgat
    20041 gcacaaaaaa cttttacgcc tttgtgtcct gacgaactgc ccgtgaacga ggggcatttg
    20101 attgtcgagg agttaaatgc ccaagccgct ttggcggatt tgcgtgtgat gacgaaagat
    20161 gcgcatcata tggcggcgaa atggcttgtg ggtaaccctg ttgatatgtt gaagccgaca
    20221 ggtttgtccg atgcggattt gacttgggtg gctcatgcga tggtcggtac gcgcggctat
    20281 gaattgttgg acggtctgcc ttccgtcaag gaatacgatt attgcgtttg gaaaggtgtc
    20341 gatcccgaat tgcatcctta cggcgcgtgt tttcacgata ttgaggaaaa actgagcact
    20401 ggattgattg agtggctgcg ttgtcaaaat acgaatatgg tcattgtcgg cggcttggct
    20461 acggattatt gtgttaaaac aacggttttg cagttactta aaggcggtcg ttggcaggtt
    20521 atcgtgaatg aagcggcttg tcggggtatt gcgcccgata ccattgaagc agcgtggcag
    20581 gaaatgcgtt cttcgggtgc gataattttg aaaaacgccg aaaaaattaa aaaatatatt
    20641 aataatcaat aacttataat tgtttcacgt gaaacaccct gtctacatat gaaaattttg
    20701 cttgtccgtt tgtccagtat gggtgattta attcacactt tgcccgcaat cgaagatttg
    20761 gcgcgacaat gctctgatgt agaactgcat tggctatgtg aagccgggtt tgcgggtatt
    20821 gcacggttgc atccgtttgt aaaaaaggtc catgtaatga agtggcggca atggcgcaaa
    20881 catctctttc aggctgaaac ttggcgggag atgggtcgtc tgaaacaggc tttgctgcag
    20941 gaagcatttg attttgtatt ggacagtcag ggtctgatta aaagcgcgtg tttcgccaaa
    21001 atggcaaaat cccctattta tggtttggat aaacacagtg cgcgtgaggg ggtggctgct
    21061 ttggcgtatg ataaaaagta tgtcgtaccg aaaggtagaa atgccgtttg gcgcaaccgt
    21121 gatctgtttg cccaggtgtt tgggtatgca atgcctgaga cgcaggtatt tggtttgtcc
    21181 gttcctgaag caagccgtct gaaaaattta gcgcagccgt attatgcggc tttgcacgcg
    21241 accagtcggg acagtaagtt atggcctatg gaaaattggc gggagttgct gcagaaactg
    21301 aatcaaaaac aacaatgcaa tgtttatctg ccttggggaa acgaggctga aaaagtgcgt
    21361 gcggaacaga ttgcagacgg gctgccgttt accatagtgt gcgctaaaat gaatttatta
    21421 caggcggcgt atctgttgaa acacgcggtt ggaatagttg gtgtagatac cggtttgctg
    21481 catttggcaa atgccttgga aaaacctgtg gtcggcattt ataccgatac cgatccgatt
    21541 aaaacaggcg ttcaagtttc ggctattgca aaaaatgtgg gcaatatcgg gcagattccg
    21601 accgcagatt tggtttatca aacgctgatg gattgtgttt cagcagataa aggctaaagg
    21661 gtcgtctaaa actgatatgg cggcatgata ttttccaatt tattagatac aggaatgtat
    21721 atccgccaga ctgagtaaat actttaaaaa tggtataatc ggtaaaatta tccgaaagat
    21781 tgctaaataa attcgttttt aaggaaaacg gatttgattt tataaagaag aaggtttggt
    21841 ttcgctggat ggtgaaagca gcgttctttt taggagaatt caatgaaagc actggtcgca
    21901 gtaaagcgcg tagtggacta caacgtcaaa gtccgtgtga aagccgatgg ttcggatgtg
    21961 gacatcggca atgtcaaaat gtcgatgaac ccgtttgatg aaatcgctgt ggaagaagcc
    22021 gtccgtttga aagaagccgg gaaagtaagc gaaattgtag cggtttcttt gggtgaaaag
    22081 aaatgcgaag aaaccttgcg taccgctttg gcaatgggtg ccgaccgtgc cattcatatt
    22141 gaaaccgatg tgaaattaga gccgctggca gttgccaaac tgctgaaagc tgttgcggac
    22201 aaagaaaatc cgcaaatttt ctttttgggc aaacaagcga ttgatgacga tgccaatcaa
    22261 gtggcgcaaa tgctggcagc tttgttgaat gcggcgcaag gtacatttgt ttcaaaagta
    22321 caaattgaag gtgatgaagt acagattgtg cgcgaaattg atggcggcga agaaacgatt
    22381 gcgttgaaac tgcctgcagt catcagcgca gatttgcgtt tgaacgagcc gcgctttgtc
    22441 aaactcccca atattatggc ggcaaagaaa aaacctctgg aaaaactgga ttctgccgat
    22501 ttggctaccg atatttcgcc tcgtctgaaa acggtgaaat ttgccgaacc taaggcgcgt
    22561 cagacaggtg taaaagtagc aagcgttgcc gaattggttg aaaaattgaa aaacgaagcc
    22621 aaagtgattt gaggaggctg aaatgagcgt attgattatt gccgaacacg acaacaaaca
    22681 gttgaatcct gccactttgc atgctgttgc cgctgccgcc aaactgggca aggtcgattt
    22741 attggttgtc ggaaacggcg catcgtccgt cgtggaattc gcgaagcaag tagcgggagt
    22801 ggaaaaagtt ttggttgcag atgctgctca ttatgctgaa ggtttggctg aagagttggc
    22861 tccgctggtt gtcaaattgg cggcggatta ccgctatgtt gcagcaacgg caaccacatt
    22921 cggtaaaaac cttttgcccc gcgtagccgc cttattagac gtaccgcaaa tttctgattt
    22981 gaccgagatt gtagataaca cgacttttgt acgtccgatt tatgcaggta atgcatttga
    23041 aaccgtgcaa gccgattcag aaaaattggt gctgaccttc cgtgtgacgg cttttgacgc
    23101 agtggcggcg caaggtggta atgctgaggt aattaatgtt aaaacaaccc ctgcccaaaa
    23161 cctgagtcgt tttgttaacc gtcaactttc ccattccgat cgtcccgaac tgactcaggc
    23221 aaaagtgatt gtttccggtg gtcgtgcgtt gggtagtgtg gaaaaattca gtgaagtgct
    23281 gacaccgttg gcggatgttt taggtgcggc aatcggtgca tcccgtgcgg cggttgatgc
    23341 cgagtatgca ccaaacgatg ctcaagtcgg acaaaccggt aaggtggttg cgccgcagct
    23401 ctattttgca atcggtattt caggtgcgat tcaacacgtt gccggtatgc aggacagtaa
    23461 ggtgattgtt gcaatcaaca aagatgccga tgcgccgatt ttcaatgtag ccgattacgg
    23521 attggttggc gatttatttg aaattgttcc acaattaata gaattattga aaaattgatg
    23581 tttcacgtga aactttcagg cgactttgtt attaatgagg tcgtctgaaa acatttcagc
    23641 atgattacga cttataaaac cattacttcc ccgacgcagg ctgagtttaa agataaaggc
    23701 agccgtttta ttgcatttgc ctatccgatt cggacattgg ctgatgtgaa aaaatacctc
    23761 aatctgttaa aggaagagca ttataaagcg cggcattggt gctatgccta tcgtttaggt
    23821 gtgaacggga tgcagtttcg cggcaacgat gatggagagc caccaggaag tgctgggcgg
    23881 ccaattttgg gacagattga ttcggtgggt atcactgatg ttttggtcgt ggtcgtccgc
    23941 tatttcggtg gtgctttatt gggcgttccc ggcttgattc acgcatacaa agaggcaacg
    24001 gctcaggcgt tggcgatcgc ggaagtggtt gaaaagaata ttgaaaaaac cgtttggctg
    24061 aaatgtgagt atccgttttt gaatgaagca atatgcattg ccaaacaata tcaggcggat
    24121 atattggagc aggatttgca gttggattgc cgattgacgg taagtttgtc gttggtaaat
    24181 tatgaggcct gtgttgcggc ttggaagaat acacggcagg ttgaagtaaa tacggaaaag
    24241 ccttttgaat gaaaggttgt atgaaatgct ttgtaatggt atttcaggca acctcttcgc
    24301 tgattaattt tcttgtgtat atctgtctaa ggtatcgacg ctggagcata aatatagcca
    24361 agccaattga tggaattcgc ccggcgcgtt ccataatgca tcttcactca taatgctata
    24421 ttcgccttct gtttgcaaat tcagttctac cccgtcttca atagtctgat ggcgcacctg
    24481 atactcgtgg gtatagaaat tgtccatatc gcgaattaaa gcgacggcat atttccaacg
    24541 gcggatgttt tcttccagtt gcagtagtaa atgacaccat tcgcggtatt tgctttggat
    24601 ttcatcaata cgtttttgcg tgtttgcttg ttctttttta atttgtgaat aagttgaata
    24661 ggttgcagag tggaatgaag tggtcttttg tttgattcta ctaaaatttc ttcagatggg
    24721 acaaaagttc ctttgtatcg agttttcgtt aaaagtacat atttgtcaag gagatggcat
    24781 gaaactgctg gttatcggta atggcggtcg cgaacacgcg ctggcttgga agttggcgca
    24841 gtcgcccaaa gtggaaacag tatttgttgc gcccggtaat gccggtacgg cgattgaatc
    24901 caagctccaa aacattgcct tgaccgcgta tcaggatttg attgaattct gccgtaaaga
    24961 aaatattgtt tttaccgtcg tcggccctga agcgcctttg gcggcaggta ttgtggatga
    25021 ttttcgtgcc gcagggctga aaatatttgg tccgacacaa tatgcggcgc agttggaaag
    25081 ttctaaagat ttcgccaaag catttatggt gaaatacaat attccgaccg cgcaatacca
    25141 aacctttgaa aatgccgatg ccgcacatga ttacgtcaat cagaaaggtg cgcctatcgt
    25201 tatcaaagcc gatggtttgg tggcaggtaa aggcgtgatt gtggcaatga ctttggatga
    25261 agcgcatgct gcgattgacg atatgttgtt gggcaataaa atgggcaatg ccggcgaacg
    25321 cgttgtgatt gaagattttc tgcaaggcga agaagcgagc tttatcgtta tggttgatgg
    25381 caatcatgta ttacctatgg cgaccagtcg agaccacaaa cgtcttttag acggagataa
    25441 aggcccgaat acgggcggta tgggtgcgta cagtcccgcg cctgtggtaa cgcctgctgt
    25501 gtacgagcgt gcgatgaacg agattatttt gccgaccgta gcgggtatga aagcggaggg
    25561 gcacgagttc accggctttt tgtacgcagg tttgatgatc gatcaaagcg gtgcacccta
    25621 tacgattgag tttaactgcc gtttcggcga tcccgaaacc cagccgatta tgagccgtct
    25681 aaacagcgat ttggcggatt tggttgaagc ggcaatagac ggcagactgg acagcgtcaa
    25741 agcggaatgg aatccgcaaa ctgccgtggg tgtggtactg gcggcgcaaa attaccccga
    25801 aacacccaag aaaggcgatg ttatttccgg tttggatgac gtcaaccgaa tcggcaaagt
    25861 attccatgcc ggcacaacgg taaacgagaa aggcgacgtg ttgaccaacg gcgggcgcat
    25921 attgtgtgtt gtaggattgg gcgacgatgt ggcgcaagcg aaagccaagg cgtatggcgc
    25981 gttggaaaaa atcagcttcg acgggatgca ataccgtaaa gacattgccg ataaagcgat
    26041 taaccgttaa ttagaaagag gatgatgccg tctgaagcca agtatgactg atgcgcttca
    26101 gacggcattt tatattatgt tattttcgag aattatcgca gcatcagcgc aacgtaagtt
    26161 aagcgtttat ttgaaaaaag gcggtttggt tgcttatccg accgaatctt gttatggctt
    26221 ggggtgtctt ccgacattag ccaaagcact cggtaagttg gcgcatttga aaaaaaggcc
    26281 gcaacacaag ggtatgattg tcatcgggaa tcaacttgaa caattgcagc ctttacttca
    26341 aatgccgttt gaaaacattc aaaccatgct tagaaacgaa tggcctgctc ctaagacttt
    26401 cctattgttg gctaaatctg gcgtattacc tgcgttgcgc gggaagaggc gcagcaagct
    26461 tgcggtcaga gtgcccgatc atacaggtgc aaggcgtttg tgtcaggcat tgggtatgcc
    26521 tttggtttcc acttcatgta atcgggcggg taaacgcgcg tgtcggacgg agagggaagt
    26581 cagaaggcag ttcggacgcg atgtgtggat tgtcggtggg agaatcggca ggcagaaatc
    26641 gccgagtcag attatcgatg gggaaacagg taaacgtttg cgttagatat ggatgcttga
    26701 gtgaaaactt ttttgcatac acaaagaaaa aattttttgg atggtatttc tctacatgat
    26761 tgttatcgca cggcgataaa ggtagaagga cgcaacgtct gctttgattt tgaggacggt
    26821 tttgtcgttc tggacaataa tccgaacaat caagcgggca agcatctgaa aacagatttt
    26881 tcccgtgttg ttttaaccca tgaaaataga aacgccgaag acgactattt ggtcgatatt
    26941 tttgaagaca tcgtcttttt agggaaacgt ttgtttacag tcagaaaatt tcttgatttt
    27001 tctgaattgc ttgaaatgat caatgcacaa ggctattttc ttgaatttct gtatctgtat
    27061 gaatgtatcg gtgtcaaaac aagcgattat tttttagagg ctgtgttggt tacgggacgg
    27121 agccgggaat gtaaaaaatg ctttatcaaa attatgaggg caagcagttt tgtgtatcaa
    27181 tggaacaatt tacgtttgaa tagtgagatt gtatagcggg tttatatttc ggatggaagc
    27241 cggtttaaaa aaataatgtg gataatttca gggaatggca aaatgtcgtc tgaaatgctg
    27301 atatttcagt attccagacg gcatttttat aataaggctt atggataaat ttgtgtataa
    27361 gttaaaatca gagttttttc ccgcatacgg tttcatattg tttttcagtt aatgtcccgg
    27421 acaggatgct catcgggcgg agggaagagg ctgtgtagtt ttgtgtggaa atttccgtgt
    27481 tttttgtatc aaataactgt agcgaactta agcggtatgt tttgttgttg cagtggattt
    27541 cccactcggc aatggcagtc ttgtatgcgg gggtgttggc aaaacgttct tgtttcagat
    27601 tggtaacaac ttttttatct tggaaaatca tcagatttcc gttttttctc acactgtctt
    27661 tattaatata tgtatgaata ttgccgtttg agattgtgcc gatattttcc catcgggcag
    27721 acttgtccgg tgtgctgcag gcggttatca aaatagggag taaaacgaaa gataggaaga
    27781 ttgggcgcat tagggtttcc ttaatcattg aaatccgctc attcagaaag aacgggttgg
    27841 gccggagggg taatgccgtc tgtgaaatga ggcaaatatg ggaaattgat gcagccttcg
    27901 tgttttacgt atttatatat tccgtatgga ggaatatgct gcttttgaag agtgtattat
    27961 ggatgaactg ccgataacag taaatcggtt tgatttgttt ccgtcagctt atgccgtctg
    28021 aagccctttc agacggcatt tattttgaag ttctggttgt ttttgccagt gatattttga
    28081 tggtttgaag ctgtctttta tattcccggc aatgcgggca gaacagcagg tgcatatata
    28141 tggaaatctt ctcgcccggg gtggtttccc ggtcctgatg tttggaaaga agcagggcaa
    28201 tatcgcggca ttttttcata acgtttactt cgggttttct tggttgaacc atttgatttg
    28261 caggcattgg cgcaatgatt ctcgggcgcg gtgcataatg gtgtggtagt tggacgtgct
    28321 gataccgcac atttgttgta tttcgttgga tgaaaaaccg agtatttcct tcagggtgaa
    28381 tacccgtgcg gtgttttcag gcaggttgta taggcagctt tgcagaattt tttggaattc
    28441 gttgttattt aatgattttt ccggagtgtt ccaatgttgc ggctgccctt ccggcgtcca
    28501 atgcccgttt tgggaaaaat ggctttcaaa tgcttcatcc agcagctcgt catccagtgc
    28561 ggtaaagact ttcctctgcc tcccgatttg acgtaatgcg tcaataattt tgtttttcaa
    28621 tatggcaaaa agccagctgt tgacaagtgc cctgccttga aaactgtcgc ctgcgctgta
    28681 tgcggacagc aatgtttcct gcactaaatc ttcagccaaa tcagggtggt cgggcaactg
    28741 gagtcgcgca aaatgcagaa gcagtttgcg cgactccatt aattcggcat cggtcaggtc
    28801 gggtagcggc atagtatcgg gaacaaggta attttatgcg gatagatggt tttggctcgg
    28861 ggaattgcct tccatatttt cggatatggc ggacaaggag gggatgataa tcctttgttc
    28921 aatccagcct gaccatttcc caagcaaaat atttttccaa ttatcggctt taggcataaa
    28981 ttcgacaagg gtttgggcga gggtgtcaaa cgaaagcgcg gaggaaccca ttatctccag
    29041 cagcatcata tcgaagccgt ccaatgtttg gtacatcaca tcatcttcgg cgtttcgcca
    29101 tattaacaag gctgtttccg cttcctgcaa atcatgggta acatcgtatc gatattgccg
    29161 gataaaggcc gcaggggagg gcgtgtattt gctgtcattt gaataatgaa tgtcgggaat
    29221 atccggaatt tgtgcaactt ctgccagcaa ttgggtatat tcaaaatcca tcagtgccaa
    29281 aatgccgtct gaaagcggca ggctttggca atattgtagg aactcgccgg ggatttcttg
    29341 aaaatagggc gtttgggcac gcgcgtcgcg gacgaaacct tctttcagac ggctccattc
    29401 tttgctgtca aagtattgcc gcgtttcggt atagcagcgg tcgataaagc tgtggatatt
    29461 gttgcgtatc aggcggatat agacgttcaa tcggtcttgc ggcagaccgt ctgcggcttc
    29521 gcccccgcgt atggcttggg agaaacggtg ctggtattgg gcggaggttt caggctgcac
    29581 ggcggtattc ctttccggca cgcgtttgat aatcggcaat tttggcaact tcggcttcga
    29641 gttcggcaaa aggtgggaaa ttgaaatcgc gttccaacag ggtgggaggg atggtgggaa
    29701 gcttggtgta ggcgagttcg agcaagtccc aaacggtcgg taaaaccgcc gcgccgtggg
    29761 tatcaatcaa aagctcgggg gtttcggcat cgtgtccggc gatgtggatg tagcacacgc
    29821 gccctgcgtc cacattctct aaaaaggcct caggcgacag caggccgtga ttgacggcgt
    29881 tgacgtaaat attgttcaca tccaaatgaa taccgcagtc ggcttcacgg gcaaccgcgt
    29941 tgaggaactc gacctcgttc atctcggcaa gcggagaatg caggtagtag gacgtgtttt
    30001 ccacggcgat gcggcagccc aaacggtctt gcacttcgcg gatacgccgc gccgtatgat
    30061 gcaccatttc ttcagtgaaa ggcagcggca acaaatcgta aagatgaccg ccgtcgtggc
    30121 agtagctcaa atgatcggag aaaaacgtgc agtcataacg gcacatcatt tctttgatgc
    30181 cgtctatcaa atcggtatcc agcggggctt gtccgcccag cgacatagat aatccgtgca
    30241 acgccagcgg cagccgttcc gccacacggt caaactgttt gcgcgccctg ccgcccattt
    30301 tcagccagtt ttccggtgcg gcttcgataa agcatatcgg gctgttttcg gaaagcgaga
    30361 gaaagtcttc cgccaagtcg cggcggtagc ccaagcctgc gtgttgaatc atatttttac
    30421 ccggttcgga tggcacaaaa gaataatgtg cttgtttaaa aaaatctttg tttgccgtta
    30481 aaaaagccgc attttactga aaaacgcggc ttggtttgaa ggggagggat tatttagaac
    30541 cgcatttgcc ttcgccgcat ttgccttcgg cagatttggc tttggcttta gatgctttgg
    30601 tgtgtttgtg ggcttttttt acagttgcac cgcatttgcc ctcgccgcat ttgccttcgc
    30661 ctgctttaga agcagccgcg ccgcacgaac cttcggcaga tttggatgcg ccgcaagagc
    30721 cttgggcgga tttttgaacg cctgttgcgt tgcttgccgg tttgtgggcg gcaacggcgc
    30781 cggcggccaa agacagggat aaagcaccgg cgagtgcggc agcaatattt ttgttcattt
    30841 ctatttcctt ttcagaatat caagttaacg agataaggtt ggaatgttta aagtcggatt
    30901 ccacgactga atcaatcttg tttcagacgg catttggggc aaaacagttt tttcagcagc
    30961 gtatccagcg accatccgcc cgcaccctgg aaaagcagcg ggattaatac cacgatataa
    31021 attaaagcca ttttataacc gttgtcgcag acattgtaac ccgaaccggc gtgaaccgca
    31081 gcccaagcga cggcggtaac gaccatcagc cccaatgccg acaggcgcgt tgccaaaccc
    31141 aaaagcaaca gtacgggcag caaaagctcc gcatacatgg cgaaattcca gtttaacgcg
    31201 tccggcagca agttgaacgg gaatggaaac tgatcgttga tttcggaaaa ccaattctcc
    31261 ccgttccatt tttgcaaacc cgattccaaa aattcatatg cggcaaacag acgcaaaacc
    31321 agcatagcgg atgccgtctg ccaagttgtt ccgattttca taagtgttcc tgtttttgtt
    31381 cctgttgata ccccgttaga cggattttgt cggaaaaact gacacccgtc agctaataat
    31441 ttaacaagtt aatgaatata aaaagaaagt attttgacgg aaagaaaggg aaaaaggcgg
    31501 gatatttgga taacggcggt gttgtgagat aattcccgcc ttggacggta tgccttcaaa
    31561 tacaatttcc gtaaaggaaa aaagaaaata ttaattttga tgatgattta acaaagcccc
    31621 tttcgcttgg ctgtcaaagg agattgttaa gaaaagcaat ctccctttga cggggtgcga
    31681 tatgtaaagg aatggaaaat atggtaacgt tttcaaaaat cagatcattt ttggcaatcg
    31741 ccgccgccgc gttgcttgcc gcctgcggta cggcgggaaa caatgccgcc cgcaagccgg
    31801 tgcaaaccgc caaacccgcc gcagtggtcg ctttggcact cggtggcggc gcatctaaag
    31861 gatttgccca tataggaatt gttaaggttt tgaaagaaaa cggtattcct gtgaaggtgg
    31921 ttaccggcac atcggcaggt tcgatagtcg gcagcctttt ggcatcgggt atgtcgcccg
    31981 accgcctcga attggaagcc gagattttag gtaaaaccga tttagtcgat ttaaccttgt
    32041 ccaccagtgg ttttatcaaa ggcgaaaagc tgcaaaatta catcaaccga aaagtcggcg
    32101 gcaggcagat tcagcagttt cccatcaaat ttgccgccgt tgccactgat tttgaaaccg
    32161 gcaaggccgt cgctttcaat caggggaatg ccgggcaggc ggttcgtgct tccgccgcca
    32221 ttcccaatgt gttccagcca gtcatcatcg gcaggcacaa atatgttgac ggcggtctgt
    32281 cgcagcccgt gcccgtcagt gccgctcggc ggcagggggc gaatttcgtg attgccgtcg
    32341 atatttccgc acgtccgagc aaaaatgtcg gtcaaggttt cttctcttat ctcgatcaga
    32401 cgctgaacgt gatgagcgtt tccgtgttgc aaaacgagtt ggggcaggcg gatgtggtta
    32461 tcaaaccgca ggttttggat ttgggtgcag tcggcggatt cgatcagaaa aagcgcgcca
    32521 tccggttggg cgaggaggca gcacgtgccg cattgcctga aatcaaacgc aaactggcgg
    32581 cataccgtta ttgacgctat gccgtccgaa cgtgtttcca gaaggcttta tccatagaaa
    32641 aaaatacccg aatcccgtac aatacgcccc taaaattttg aaagaacgaa agaatcatgg
    32701 aagccgaagt aatcaaccag ctcaacaaca ccctgaatga tttggagaag cgtagcgaag
    32761 acatccgcgt ttatatggat tatcagggca agaaagaccg attggaagaa gtcatcgggc
    32821 tttccgaaga ccccgagctt tggaacgacc cgaaacgtgc ccaagaaatc ggcaaagaga
    32881 gcaaaatcct tgaaggcatc gtgctgacgc tcgacaacat cgcttcgggt atcgaagaca
    32941 accgtatgct gattgaaatg gccgtcgaag aaaacgacga agaaggtttt gccgccgtga
    33001 aggaagacgt ggcggggctg gaaaaacaga tggcggattt ggagttcaaa cggatgttca
    33061 accagcccgc cgacccgaac aactgcttta tcgacatcac cgccggcgcg ggcggtaccg
    33121 aagcggaaga ctgggcgggg atgttgttcc gtatgtacag ccgctatgcc gagcgcaaag
    33181 gcttcaaaat cgaaatcctt gaggaagacg acggcgaaat cgcaggcatc aaccgcgcca
    33241 ccatccgagt ggaaggcgaa tacgcttatg gtttgctgcg taccgaaacc ggcgttcacc
    33301 gcttggttcg ctactcgccg tttgattcga acaacaaacg ccatacctcg tttgcctccg
    33361 tgttcgttta ccctgaaatc gacgattcca tcgaaatcga aatcaacccc gccgatttgc
    33421 gcatcgacac ctaccgtgca tcgggcgcgg gcggtcagca catcaacaaa accgactccg
    33481 ccgtgcgtat tacccacgag ccgacgggga ttgtggtgca atgtcaaaac gaccgttctc
    33541 aacacgccaa caaagccgcc gcgatggaaa tgttgaaatc caaactgtat gaattggaaa
    33601 tgcgcaaacg caacgaagag aaacaggcat tggaagaagg taagtcggat gtgggttggg
    33661 gcagccaaat ccgttcgtat gttttggatt cctcacgcat caaagacttg cgtacaggct
    33721 acgaagtcgg caacaccaaa gccgtattgg acggcgactt ggacggcttt atcgaagcca
    33781 gcctgaaaca gggtgtgtag tttaggctgt atgccgtctg aaacctgtac cgtttcagac
    33841 ggcatttttg cgtttcgtgt tataatccgc gccgcacacg ggacgggcag acagtcgccg
    33901 cgtatcgcgt aaggcatacg gggaggaaag tccgggctcc gcagggtaga atgccggtta
    33961 acggccgggc gcggtaacgc gacggaaagt ggaacagaaa gcaaaaccgc cgatggctgc
    34021 tttggcagca caggcaaggg tgaaaaggtg cggtaagggc gcaccgcgca tttggtaaca
    34081 atatgcggca ggccaaaccc cattcggagc aagaccaaac agaacgcaat gacgctgccc
    34141 gccgagcgtt cgggtaggtt gcttgagcat accggcaacg gtatgcctag aggaatgact
    34201 gtccgagaca gaacccggct taccgcctgt ccggtgtgca tccgacacgc aaatgccgtc
    34261 tgaaacctga aatacggttc ggacggcatt tttatttagg ctgtgttttt atattccgtt
    34321 gatgcagccc tgatgagtcg gacaaaatgc cttttactca ataggaccgg tttccgccca
    34381 agcggaaata aacggctatt tcccttgcaa acagacggaa tcaggcgggt atttaaatga
    34441 caatcggcag gctgccttcc ggttgggcgg gagcctgtcc tgtcgggtgg aaacggtttg
    34501 gtttcattat gcggaaaaat gccgtctgaa gggttcagac ggcatcggcg catcggggca
    34561 ggacttagtc tgtgccttcg agcattttgt tcaatttggg gacgagcagg agcagcagga
    34621 agcctgtggc tgcgccgatg tagaacagca gccggtagaa gccgatttcg tcgcccgcct
    34681 gatagccttt ttcaaacaat acgccgccca aagtgaagcc taatgaaaag gcaaggaaat
    34741 taagggcgac catttgggtt ttgaataaag gcggtgcgat tttggtggag atggacagcg
    34801 caatcgggga aatcatcagt tcgcctatcg tgatggcgag gacgatcagt gcgaaaaccg
    34861 caataggcat cggcgttccg gaggaaataa agggaacgaa tcccaaaaac gacgcgccgg
    34921 taacaaatac cgccatagcg aatttcagcg gggttttggg ttgtttgcgc cccatttttg
    34981 tccacattgc cgccatgagt ccggaaaaca ggatgaccca caggctttgc atagaatctt
    35041 tccaggcgac gggtacggta aacgaaccga tggtgcggtt gacggtttcg tcgaaataga
    35101 cggttgccac ggtgtaaatc tgaaaccaga cggcccaaaa catacagatg gtcaggaaaa
    35161 gcgggatgta ggcgatgatg tgccgtttgt tgtcggaact gacgcggggg ttggtcagca
    35221 ggcgggcgaa ataggcgatg acggcaagga tgacggtaga taagaggatg ccggagaaat
    35281 tgtcgaggtt gacaagcccg gttttgatgg cggttgcaag tgcggcgatg agggtgatgc
    35341 cgacggcggc cgcagttttg ccctgtcctt ttgaaagcgg atgggggacg gtggggtggg
    35401 gcaggttttt acgtcccaag gaataacgcc acaagccgaa tgccataccg accgccgccg
    35461 cgccgaaacc ataatggaaa ccgatgtttt cttgaagcag tccggtcaat aaggggccta
    35521 agaagccgcc gatattgatg gcgatgtaga aaatggaaaa tcccgcatcg cgcagcgggc
    35581 gcatttcgtc ctgttcgtat aatgcgccca ccatagaact ggccgtagat ttcacgccgc
    35641 cgctgcccaa tgcgatgaat atcagcccga ttagaaggcc gtacaggccc ggtgcggcgg
    35701 caaggacgat gtgcccgagc attacgacga tgcccgagag gaagagggtt ttttccgcac
    35761 cccatacgcg gtcggcaaac cacgcgccca aaatggtgga caggtacacg ctgccgctgt
    35821 atgcgccgac aatgccgcct gcgagggttt tgtctatgcc caagccgcct ttgtcggcgg
    35881 tgtagtagag gtaaatcagc aggatgccct gcattccgta aaatgaaaaa cgttcccaca
    35941 attcgatatg gaagagggtg gaaagctgga aggggtggcc gaaaaatgtt ttttctccgg
    36001 tgcgggcggg atgccgggac ataaaggttc tcctaaaaga ttttctctca ataccgtctg
    36061 aaacggatgc ggacggcggc ggtgtttttt tgtatgaaaa tatcggtttt taaccgatat
    36121 tttcattctt tgtcaaacga cgcgctgccg tttttcgcgg gcggatgttt ttatatttgt
    36181 ttcaattcaa tggattgtat tttagaggac gtgttccgat acggcgcggg taaatccttt
    36241 tctgtcaatg gcttatccga tagggcggtt tttacttaga tgggaaatca cttccttata
    36301 tatccgccac gccgaagggc gcattatggt ggattaactt taaaccggta cggcgttgcc
    36361 ccgccccggc tcaaagggaa cggttcccta aggcgcccaa gcaccgggcg aaccggttcc
    36421 gtaccatttg tactgcctgc ggcccgccgc cttgtcctga tttttgttaa tccgctatat
    36481 ttttcttaac catcccttcc aacagccgtg cgaaggtttt tttatatcag tgggaaatgc
    36541 aatattctat tgtttttatt gtagaatttt aaaaacagat tgttgtgttc cgcgtttttg
    36601 ccggtttgga agcggtgggg cgcatcagcc ttttataaag gcatatcgga agcctgtata
    36661 aggtttttga acatatcgat cctgttccct tgcaggcgga cggcagcggc ggatggcgaa
    36721 aggcgggttt tcaaatgtcc ggtttggcgg cgcgtctttc agacggcatg gcggcggagc
    36781 gtgccgcgcc gtctgaaaac cgtttatttc cctttgccgt ttcctgcgga gtcttttttg
    36841 tccgaaccga agaggcggtc gaaacgtatg gtgtatgtca gctcgccgcc cgacgaacgg
    36901 ctgccgatac gggcaaccgc ctgtatggcg cgggtcagcc ggtaaatcag tttgacggac
    36961 tgttccgcgc tggagatgcc gtattcgtag ccgatgtaga gtttgccggt cagttgtttg
    37021 ccgacggtca gcacctgttc ggcggggttg agttcgccgg tttgcgcgtt gcggctgcgc
    37081 ttgctggtaa agcccaaatc atccaccagc ccgatgcggt cgttgatttg cccggcaagc
    37141 agcgcgcctg cggctgcgga cagggcggca ttgtcgccgc tgctgccgct gccggcgcgg
    37201 ttgaggatga gccaggagag cttgtctttt tcactcatcg gttcgtttgc cgtcagcgta
    37261 atgcgcgggc tgttgaggct gcccaatatt tccacgcccg caccgacggg ggaaaggcgg
    37321 cgttcggcgc ggatgttcag gttggggtcg ttgagcgggc cgacaaagga gactgtgcct
    37381 ttggtaatgt ctaaatcctg cccgtatgct ttgtaacgcc ctttgatgac gcggaccgtg
    37441 cccaccccac gcacatttcc gcccggttgc gcggtcaggg tcagtttgcc gcctatggta
    37501 acgtccgcgc cgtagccgga gaagcggatg ccgtcattga ggtctaaagt caggttcata
    37561 ttgacgggga gcgatgccgc cgcctctttc ttgacttcgc ccaatacgac gacatcgtcg
    37621 ccgacggacg gcatcgagga tttttgcgaa ccgaacagcc cctgatcagt tttaatcata
    37681 ccggtaacgg atatgccttt ttgcggcgaa tagcgcaggc gggtgttgcc ggaaaccgtc
    37741 aggcggcggt tggggcggga caggatgcgg tatttgtcga acaccgcgcc gatatcgaca
    37801 tcgggcacgc tgttttccat gctgaccgtg ccggagagtt ccgccgtccc ttcgtgccgg
    37861 aatttcaggc tgtcgattac ccatttcctg cctgcaatat gcgaacgcag cgagccgttg
    37921 tccaagatga tgccttgggt ttggttgcgg taatagagct tgtcgccgtt gatactgccg
    37981 cccaagtgcg ggtcggcgat gctgccgccg agggttacgg cggcattcag gctgcctttg
    38041 acggtttgtc cgaccggtag gaagttgcgg aatacttcgg catcggcaac ggtcaggttg
    38101 agcctgccgc ccaaaggcgc ggtatcgaag gagcggcttt gcccgacggt gatattgccg
    38161 ttgattttcc cgtagttgct gctaccgttg acggcggcat tgacggacgg agagcttacc
    38221 cgtccgccga tttgcgcgga ggcattcagg ctgccggtaa tgttttgcgc ggcggcgggc
    38281 agaaagggct tcaatgcgcc caagtcggga agggaggctg taatcctgcc gccgagcggt
    38341 gtatttgcca tattgccgcc gaaggcgttg ccgatgccca aatcggcgtt aatccgtccg
    38401 aaacgcgcgc cgccgtcaag caggattccg atgcggtcgt tttgaaagcg cgttttcagg
    38461 gaaaatgcgt tcaaacccaa agcctgcccg ccgggcaata cggcatcgcc gctttgccgg
    38521 ctgatattga ggtagccgcg cgcgttgtgc ccgtaggcaa catcccagtc gccgtttaaa
    38581 accagattgt gttcgaaggg cggtttgaag aaattgtgca actcggcgat gtgcaggccg
    38641 cgtgcgccgc cttttgccga tatgccggtt ttcctgtccc aagaaaagtg ttgcaggttg
    38701 aggctgccgc ccattgcctg ccaatttgcc gcacttgccg ccacgcgttc cgcaccggct
    38761 tcgagcgtca tacggttttg cagcttgagg ttgaatgcgc cgccgatgtc gaggatgccg
    38821 atgctgcctt tccatcgggt aagttccctg ttgatgccgc ctgaagcgtc caaatcgagt
    38881 ttgaacggtt tgccgtccag cgtcatggcg gcgtgtgtgc ggatgcggtg ctgcgcgccc
    38941 gtaccttcca gcgtcaggcc ggcggtatcg acaaccgccg cgccgcccga cagggaaagg
    39001 cggccgccct tgatatcggc gcgcatcggg cggcttgtgt cgggcgagcc tttgagggta
    39061 aaatcgagcg aacggatgtc tgccgctttg ccgatgtgta agttgcgcgc cgtgccggaa
    39121 aggtcggttt caaaggttcg gatgccgccg tccaaatcgc cggaaaggtg tccgcgtaca
    39181 tttaaagacc ccgcgagtcc gaaaccgaaa cgggataaat cgggtgcggt gatattgagg
    39241 ttaagccggt cgcctttttt gccgaagccg ccgtctgttt tgacgatgtt ccgccccaac
    39301 cgcaaatcga cggcggcacg cggaaggtgg cgggactcgt aaacaatgtc ggcactgccg
    39361 gcaatcggca cgccgttgaa cgtaccgggc aaaaaacgca ttttgcccgt aaatttctct
    39421 tttgccagtt caccggcaag atgaatcgaa ccgttgatat tgcctgccgg aaattgcgga
    39481 tcgatgcgcg aagggtcgaa tgcgcgggaa cggatgtcca gcttgagcag gcggtcttta
    39541 aacagctcga gatagccttg cgcggtcagg ctgccttccc cggcggagat gttgacggtg
    39601 tcgaacacca gtttccgctg ttcgtttgcg gggtcgcttg cgatggcgag gctgccgtcc
    39661 gtgcgtgccg tgccggtgcc gagttgccaa gagattttgg gcgaggcggt cgtgccgccg
    39721 atgccgatgc tgccgtccaa cctgcctttg aacgcggttt gcagcacgtc ttccgcgccg
    39781 acggagttga tgcctatatt taaatcaagg atgtcttttt cggtgtcgat tttgcccgac
    39841 agcctgatgc cgccccgtcc gagcagggcg gcggacgtat tgccgatatg caccgtgccg
    39901 tcctgccgga tgacaaagcc gcccaaaacc tgacggacgg ggatgccgtt gcggtcggca
    39961 aagtcggctt tggtgttttc caaatcgagc gagccttcca gcgcgatgcc gtctgaaaac
    40021 gacgggatgg cggtcaggtc gaaattcagc ccggcatcgg gcagggaagg cacgaaggcg
    40081 gacggattga tgttgaatcc tttgaccagt acttcttcca atgttttatc caatgattcg
    40141 gcaaacgggt ggatgacgga ttttcccgag aggcggatat tgccgccgtc gatcgtcagt
    40201 tcggcgcgca catccttcag gctgccgctc agccgcgccg tactgtgtat ggtttcgcct
    40261 tcgaatccgc ctttggtgta aatggcggta tcgagggcaa acggtttttt caagccgacc
    40321 gaggctgacc ccgacgaact gctccacggc gtgtcggcgg ccttcaggtc gaggcggtgc
    40381 cctttacggt cgtaacggta tgccgcgttg aggcgttcga gatagacagt ttgtttgtca
    40441 aaggttttgc ccatgctgat tttgcccgtc tcgaagcggt cgagatagac ggtggcgggc
    40501 aggtctatgc tgtcgggcag gccttgaggc gggcgttctt ctttaggcgg agtcggtttg
    40561 gttacgatgg cgatgtcgcc ggcggagatg tcggtgatgt gcaggctgcg gcgcatcagt
    40621 tcggacggtt tccacgcgaa gcggaagcgg ctgattttaa ggtctgcccc ctcggtttct
    40681 atcgaccagt tgtcgccgtc gaagccgtcg agcagtgtgc ctttgaggtt ttgggaggaa
    40741 atgtttacgc cgaaccagga cgggatttgg tacagcccga agcgcaaacc tgcttccgta
    40801 ccggcgatcc agccgaggaa acatactgcc aaaatcagga cagacagcag tgccgccgac
    40861 agcttcagca gcgggcggcg tttttttgcc ggcggggcgg ggcggtgttc agacggcatt
    40921 ttgcgcgtgc cgttttcggt cggatcggta tctgtcggtg cggtatcggt cataatgtct
    40981 gtttcaaaat ggtttgctgc aatgccgtct gaagcggccg tatcggttta gaaacgcgtt
    41041 cccaagctga tgtgccagcg gatttttttg tcgctgtgtc cgtaggcgat gtcgaaggaa
    41101 aacggcgcga gcgggctgaa ccagcgcacg cccagtcccg aaccgtgttt cagcttcata
    41161 cgtttgaaat tggcggcggc atcgcccata tcgtggaata ccgcgccgga cagggtgcgc
    41221 gtaaacggca gttggtattc caagctgccc accaagaggg cgcgttcggg caggaccgat
    41281 ccgttcggac cggcaagccc gatgctgtca agttcgtaac cgcgcacgga agacgcgccg
    41341 ccgctgcgga acatcagccc cgaggggaca tcggcattgt cgcgtgcaac ggtgtaaccc
    41401 gcttgtccgc gtatgatgaa cgtgccgagt tttttgtttt cgggcgtgaa gaaataacct
    41461 gcgcgggcag aggtgcggat tagcgcggtg gaggacagga atgtgcccaa agtcgtcccg
    41521 attttgccgt cgaggtaatg gccgttttcg gggtgcagca cgttgttgag cagctggcgt
    41581 ttccaagagg cggtcagcat cgtggcgtgg ctgttgccca aatcgacatc cgagccgggg
    41641 attttccggc cttctgcgag aaattccgcc cccagcctgg catcgatgcc cgcgcggtcg
    41701 cgcacatacc agatgccgcc ggagaaggcg cgtttttcaa ggttttgggt ggtcgaacgg
    41761 ttgtaggaaa cgttgcttgt ccagtagttg ccctgatagt tgcgcggctg gctgatgccg
    41821 gcggcaagcg tggtttcgta tttgtccata tcccagacga ccgagccgat atagcctttg
    41881 ttgaagaggt tgtaatagtc gtaggcgatt ttgccgccca aaccgtattc cgaatcgagg
    41941 cggatgccgg tttcgagttt gtggcgtttg acctcggtta cgctgacttt gacggggacg
    42001 cggtcgcctt ggaggcggtc gaagtcggct tgtacggacg cgccggaata atgcccgttt
    42061 tgttcgagcg cctgttggaa gtcgagcagc aggtcgaggt cgtagggcgt gcccggttgg
    42121 aagcgcgcca ggccggagac ggtttgttcg gggtaacgct gtgtgccggt gatttcaaag
    42181 tcgccgaagg cgatggggcg gccgctgtcc acgacgacgt tcaaatcggc ggtggcggta
    42241 tcggggttga cggccgcccg ggtgttgccg agcttggcaa gcgggtagcc tttgcgcgtt
    42301 accgcgccga ggacggaagt tttgctgttt tcccaactgt cctgatcgaa atcgctgcct
    42361 accggctgct gccagttttc cagcgcgttg cggtagtatt cggcgaggtt gccgtctgaa
    42421 aggatgtcgc cgaggatggc gacgccgacg ttggcgattt tggtgcgcgg gcccggtgtg
    42481 atgtgcaccg tataagctcc gtctttttcc gtcaggctga ccttgctgct gaaatagcct
    42541 ttgctgcgga gcatggtttt gacgttgtcc ggtgcttctt cggcaaggaa tcccgtctgt
    42601 tccttatcca aaacctcttc ctgctgctgc gtgatgagcg gcaggtgttc ttcgaccata
    42661 tctttgattt cactgtcctg cgtgtcgatg cggacgggga atttgggttt taatttgact
    42721 gattcggtgt cggggctttt gcttttgaac aatgcgaaac ccgccgcctt gttttcggaa
    42781 aggtcggcgg caggcgcgta tgcgtgcgga aagaaaaata aagccggcag gagcagggcg
    42841 gtcggtttga tcatcatggt acgggtgtcg tgcatcggtg tggcggattt cgattctaac
    42901 actgttcgga cggggcggga cgcttcagac ggcatactgc cgacggtatt tcggaggttg
    42961 cggcccgccg gaaatgtatc tgcttgtttt aaggcatttt gtttccaatc cgcatatgga
    43021 aaacaggctg cgtttcggaa tagtgtcgaa aaataggcag gattaaataa atttattctc
    43081 gttcaataaa ttaaaccgtt gcgccgaatg ataaaacaaa gcctgtccct gcatagggca
    43141 aatcaacgca cctcaaaaca cagcttgaaa aataaaaaaa acacagagaa tgagttttct
    43201 tttaaaatac aacatttttt aacatatcac aacataagga agaattatgg cgttcggcaa
    43261 atccttattt catgcgatcg gccgcgtcag cctggtcagg cagattgccg ccggtttggc
    43321 gttgggcatc gtaatcggtt cggtttcacc gcaactgggc ttggcggcag gcttgttcgg
    43381 cagcctgttt gtcggcgcgc tcaaagcggt cgcgccggtt ttggtattta ttttggtggc
    43441 ggccacaatc gcgcagcacc aaaaaggcaa caaggcgcat atcaggccga ttatcgtcct
    43501 ttacctcatc ggcacgtttt ccgcagccct gaccgccgtc atcgccggta tggttttccc
    43561 gacgcacatt gttttggcgg gcgcgggcga tgtgtccgcc gcgccgcctt ccggcattgt
    43621 ggaagtgttg aaatcgctgc tgatgaacct ggtcgccaac ccgattaacg cgattgccaa
    43681 tgccaactat atcggcattt tggcttgggc tttggttttg ggcgcggcgt tgcggaatca
    43741 cggttcggac gttacgcggc aggtcgttgc cgatttggcg gaagcggttt ccaccgtcgt
    43801 gaaatggatt atccgttttg cccctttggg tattttcggg ctggtgtcgt ccacaatcgc
    43861 ggagacgggt ttcggcgcgc tggcgggtta tgcgaagctg cttgcggtgc tgttgggctg
    43921 tatggcgttt atcgcgctgg cggtcaatcc cgccatcgtg tggtggaaaa tccgccgcaa
    43981 cccttatccg ctggtgttta cctgcctgcg cgaaagcggc gtgtatgcct tctttacccg
    44041 ttcttccgcc gccaatatcc ccgtgaatat ggctttggcg aaaaagctgg ggctgcacga
    44101 agacacttat tcgatttcca tcccgttggg ggcgaccgtc aatatgggcg gcgcggcgat
    44161 tacgattacc gttttggcta tggcggcggc gcacacccaa ggcatacagg ttgattttgc
    44221 caccgcgctg ctgttgagcc tggtggcgac ggtcagcgcg tgcggcgcgt ccggcgtggc
    44281 cggcggttcg ctgctgctga ttccgctggc gtgcagcctg ttcggcatca gcaacgatgt
    44341 cgccatgcag gtggtcgccg tcggcttcat catcggcgtg attcaggatt cggcggaaac
    44401 ggcgttgaat tcttcaaccg acgttttgtt taccgctgcc gccgatttgg gccgacagag
    44461 aaaccgtgcg gaataaacca agctatgccg tctgaagccc gtttggcgtt cagacggcat
    44521 atttttacaa ttttatgggc atatggcgga aacaataccg gctgccttga atatgtttca
    44581 acacacaggg cgatacataa agcgtcgccc tatgaaggag atgatgatgt ttaaacgcag
    44641 tgtgattgca atggcttgta tttttcccct ttcagcctgt gggggcggcg gtggcggatc
    44701 gcccgatgtc aagtcggcgg acacgccgtc aaaaccggcc gcccccgttg ttgctgaaaa
    44761 tgccggggaa ggggtgctgc cgaaagaaaa gaaagatgag gaggcagcgg gcggtgcgcc
    44821 gcaagccgat acgcaggacg caaccgccgg agaaggcagc caagatatgg cggcagtttc
    44881 ggcagaaaat acaggcaatg gcggtgcggc aacaacggac aaccccaaaa atgaagacgc
    44941 gggggcgcaa aatgatatgc cgcaaaatgc cgccgaatcc gcaaatcaaa cagggaacaa
    45001 ccaacccgcc ggttcttcag attccgcccc cgcgtcaaac cctgcccctg cgaatggcgg
    45061 tagcgatttt ggaaggacga acgtgggcaa ttctgttgtg attgacggac cgtcgcaaaa
    45121 tataacgttg acccactgta aaggcgatcc ttgtaatggt gataatttat tggatgaaga
    45181 agcaccgcca aaatcagaat ttgaatcgtt aagtgatgaa gagaaaatta agaaatataa
    45241 aaaagatgga gaaaagttta ctggtttggt tgctataaaa gttgaaaata atgggttgaa
    45301 taaatatacc attatttatc aagctcagcc tactcgttct gcacggtcga ggaggtcgct
    45361 tccggccgag attccgctga ttcccgtcaa tcaggccgat acgctgattg tggatgggga
    45421 agcggtcagc ctgacggggc attccggcaa tatcttcgcg cccgaaggga attaccggta
    45481 tctgacttac ggggcggaaa aattgcccgg cggatcgtat gccctccgtg tgcaaggcga
    45541 accggcaaaa ggcgaaatgc ttgttggcac ggccgtgtac aacggcgaag tgctgcattt
    45601 ccatatggaa aacggccgtc cgtacccgtc cggaagcagg tttgccgcaa aagtcgattt
    45661 cggcagcaaa tctgtggacg gcattatcga cagcggcgat gatttgcata tgggtacgca
    45721 aaaattcaaa gccgccatcg atggaaacgg ctttaagggg acttggacgg aaaatggcgg
    45781 cggggatgtt tccggaaggt tttacggccc ggccggcgag gaagtggcgg gaaaatacag
    45841 ctatcgcccg acagatgctg aaaagggcgg attcggcgtg tttgccggca aaaaagatcg
    45901 ggattgatgt tgatgccgcc cgaagcgcag gtattcatta cggcgggacg acgcgttcaa
    45961 atcatcaaac aagcccccga aaaaggcggc atcccaaaat ccggcactga aattgccgcg
    46021 cgtatcggcg gcattccgca gcctgccggg gaaaccgtcg gcagcagccg gaatgtcggg
    46081 tatttccgcc cgcctttttc gggctgttga aatatgaaaa taaagcatag gccgtctgaa
    46141 tgttcagacg gcctgttttt ttaacggctg tgtttcaaca ctccgccggt tccaaccggc
    46201 agcagcaggg agataatgcc ttgaaccccg ctcttcgggg cggcagggtt cgggccggtt
    46261 tggggaaagg catcgccttt ccgaatcggg gcggtgcaac ggggtgcgcc ttatgtgccg
    46321 aaagggtttg cggcaacggt atgccgtctg aagcgggtgc gttcagacgg cattgtgatg
    46381 tttggggcgc gggtccggtt caaatccgca tcagttgata aacccgttgt ctttcatttt
    46441 cttttcggaa aaatactggc cgcataccag cagggcggcg aggatgatga caaacagccc
    46501 tgccatagtc agtgtgattt tgaagaacaa ttcggcttcg agaaaagaaa accaaagttg
    46561 cacaatcagc agcagcaccc agccggcaaa cagtccgaga caggcataaa cgccgagctt
    46621 aaacacggtc ccgctccaaa atgacgacgt aggcttcgcg tttgaaaaac agcagcatac
    46681 ggcgcaaatc tttttccatc gttacgaccc gccagccttc gggggtttgt ttgttgagga
    46741 acgcgctgaa attgacgggg ttgacctttg ccgcgccgaa aaacaggctg gacaacaggc
    46801 tttcctgata aatgacgact ttgtattctt tcatcgtatt tccttttcgg ttgaaacccc
    46861 gccctttggg gcggtaggat cagactttat ttgggaggga accccttcca aatcaggacg
    46921 atacataggt tggtgcttta tgtatcgtcc tgtgtgttga aacataggat tcctttaatc
    46981 aagggattgc cttttcaggc aatcccttaa attgcagcga tgcaatgcgg cgaaatttat
    47041 tgcttattgc ccaactgcgg caataccgag ccttcgccca gcgagagcaa accgctttgc
    47101 gaataaatac ccagtttggc gcgggtgtcg gtgatgtcga ggttgcgcat cgtgagctgt
    47161 ccgatgcggt cgagcggtgt gaacgccgca ccttcgactt tttccatact caggcgttcg
    47221 ggttggtagg tcaggttggg cgattcggtg ttcagaatcg agtagtcgtt gccgcgccgc
    47281 agttcgaggg taacttcgcc ggtaacggct ttggcgaccc agcgttgggc ggtttcgcgc
    47341 aacatcaagg cttggctgtc gaaccagcgg ccttggtaga gcaaacgtcc gaggcgcagg
    47401 ccgttgatgc ggtattgttc gatggtgtct tcgttgtgga tgccggtaac caagcgttcg
    47461 taggcgatgt ggaacaatgc catgcccggg gcttcgtaga tgccgcgcga tttggcttcg
    47521 atgatgcggt tttcgatttg gtcgctcata cccaagccgt ggcggccgcc gatgcggttg
    47581 gcttcgagga agagttcgac ggggtcggcg tattctttgc cgttcaatgc aaccggcacg
    47641 ccttcttcaa agcgcacgct gacttcttcg ggttcaattt cgacgttttc gtcccagaag
    47701 gcaacgccca tgatgggctt aacgattttg atgcccgagt tcaaaaattc caagtctttg
    47761 gcttcgtggg tcgcgccgag catattggaa tcggtggagt aggctttttc aaccgacatt
    47821 ttgtagttga agccgttggc aatcagaaat tcgctcattt cgtgacggcc gccgagttcg
    47881 tcgataaatt gctggtcgag ccagggtttg tagattttca gcgcgggatt ggtgagcaag
    47941 ccgtagcggt agaaacgctc gatgtcgttg cctttgtagg tgctgccgtc gccccagata
    48001 ttcacatcgt cttctttcat ggcggaaacg agcatggtgc cggtcacggc gcggcccaga
    48061 ggcgtggtgt tgaaataggc gatgccgccg gtggaaacgt ggaacgcgcc gcattggatg
    48121 gcggcgatgc cttcgtgtgc cagttgtgcg cggcagtcga tcaggcgggc gttttccgca
    48181 ccgtattcca ttgctttttt gggaatggcg ttgtaatcgt cttcgtcggg ctggccaagg
    48241 ttggcagtgt aggcataagg cagcgcgcct ttgagtttca tccacaacag cgcggcagag
    48301 gtatcaagac cgccggagaa ggcgatgccg actttctgac cgacggggag gttttgtaaa
    48361 atagtatggt tttggttcat gaaaaaatcc tcgatagctt gaaaaatcaa agccattatc
    48421 ttataaatag aaaatattgt cagccatcat gccgtctgaa cgggtattcc gccgtttcag
    48481 gcggtattta aaaccgaaaa cattatgaac gcgttcaacc tcatcatcat cggagacgaa
    48541 atcttacacg gcagccgcca agacaaacat ttcgcctttt ttaaatccct gctcgaatcc
    48601 aaagggctga agctcaatca ggtgcaatat ctgcccgacg aacccgattt gctggtcagg
    48661 caactgcgcc gcagcttttc agacggcata ccgacgttcg ttaccggcgg catcggcgcc
    48721 acgcccgacg accgcacgcg ccaaaccgca gcggctgctt tggatttgcc cgtcgtccgc
    48781 catcctgaag ccgccaagtt tatcgaaggc ataacccgga aacgcggcaa gccgctcgat
    48841 tcgcccgaac acgcccaacg cctgaaaatg gcggatttcc ccgagggcgc agagcttgtg
    48901 cccaacccgt tcaacaacat cgccggattt tccatccgcg agcattattt cttccccggc
    48961 tttcctgtga tggcgcaccc gatggcggaa tgggttttgg aaacttatta cgccggccgc
    49021 ttcaaccaaa ccgaacgcgg cagccgcagc gtgtatgtgt ttgagcagcc cgaatcgcgc
    49081 attacgccga ttatagagca tatcgagcaa acttatcccg gcgtgcgttc gtacagcctg
    49141 cccagcgtcg gttggacgca ttcagacggc acgcaggtca aaccgcacat tgagttcggc
    49201 atcaaagccg aaggagaagc cgtcaacctt ttggacgcgg cgtgggcgga agtgttgcac
    49261 agcttggacg gattgggagc aaagctgaaa aatcgggtaa actgaaaact gtttgcccaa
    49321 atcggacgat atttaaggaa aaccatgcaa taccgaatca gaagagaaaa cgaagcaccc
    49381 gaagcaaaaa atgcggggga aaccttatgg gagcgcgaca ttatgcgcga agtgctgctg
    49441 tccgcctatc gggacaggcg cagggagcgg atgtggaaaa acatctggcg cgccgtcagc
    49501 accctgattc tggttgccct gattgcaggc attttccgaa aagacgaagc cgcattgcag
    49561 ttggcgggca acacgccgca taccgcagtc gtcaacctgt acggcaaaat cggcaacggc
    49621 gtagaagacc aggtcaaaaa actcaaagac gggatggagg ccgcctacaa aaacccgcag
    49681 gcaaaagcca tcgtcatccg cgccaacagc cccggcggtt cgcccgtcgt ctccaacacc
    49741 gcttttgaag aaatacgccg tctgaaggcg cagcaccccg gcatccccgt ttatctcgtg
    49801 gcagaggata tgtgcgcgtc cggctgctac tacatcgcgg cggcggcgga caaaatctat
    49861 gccgacccgt ccagcatcgt cggcagcatc ggcgttatcg gcagcagttt cgacgcgacc
    49921 ggcctgatgg aaaaaatcgg cgtaaaacgc agggttaaaa ttgcgggcag caacaaaggc
    49981 atgggcgatc cgttttcgcc cgaaacgccc gaacagtcga aaatctggga agaaatgctg
    50041 accggcatac acggcgaatt tatcaaagcc gtcaaaaccg gacgcggcgg ccgcctcaaa
    50101 ttccgacaat atcccgacgt gttcagcggt cgcgtgtaca caggcgcgga cgcgctgaaa
    50161 gtcgggctgg tggacggact cggcaatatt tacagcgtgg cgcgagacgt ggtcaaagct
    50221 cccgatgtgg tggattacac tccgaaggac gatttcggca gaatcttggg cagacgcttc
    50281 ggagcagaac tcaaagcaag cgtttcggaa gctctgcagg ccgtccggta gcgttgttta
    50341 cagtatttct aagggaatac cagaataaaa atatacacaa aaacactcac ctccccagta
    50401 tgctgccact aagtcagccg cgttactatt taccgtgcac ttaaagccgc aaaaggcagg
    50461 ttgtccccgg gaggttttgt gcatcctgat accgatcggt tgctgttgct gttgctgttg
    50521 ctcaaaggac ggaaggccgc cgataaacga gattacctgt ttgtcgctat tgacgatttt
    50581 tatactctgc cattttgccg gacaaaaccg cagacagtgc tgccaagttt ctgaccgaac
    50641 atctggctga cccctgcttg tacctggttg agtacgttta ctctgacaat gataggtaat
    50701 ataaagagcc gtccaacatg ctttcggtgc agcttgttat gataatggga ttggttggag
    50761 gcttgcccga tttgcttgtc cgcagaccaa cggtaaggcg gagcgggtta tccgtacctt
    50821 gatggagatg tggcatgagg aacagtcgtt tgacagtccg gaacatcagc aaaaggagtt
    50881 gtgctgcttt attaatttag gcgctgtcct agataactag ggaaattcaa attaagttag
    50941 aattatccct atgagaaaag ccgtctaagc cggtataaac aaaataaact cattgggcta
    51001 tttgtcgcag gtgtaactgc aagaacagcg gcagagttgg taggcgttaa taaaaatact
    51061 gcagcctatg attttcatcg tttacgatga ctgatttatc aaaacggtcc gcatttggaa
    51121 atgtttgatg gcgaagtaga agcagatgaa agttattttg gcggacaacg caaaggtaaa
    51181 tgcggtcgcg gtgctgccgg aaaagtcgca gtattcaggc ttctcaaacg aaatggcaag
    51241 gtttcctttg ccgtccgtta tgtacgtttc gtaacattcg aagaattcct gaaccctgcc
    51301 cgccgtttcg gtttccgtac ccccctgtta gataaggggg gaagattcga agcggttgcc
    51361 ggcttcctgc cgtccgccgg cgcgtccgtc atcacgccgg caaccgcctt tgtcatccct
    51421 tgcttatctc ccatgtgcga atcctcaaaa acgggcaaaa aaaaagccct gttacttgta
    51481 gaaagtaaag ggcgttaatt tttgttaatc gccccttctt agggacgcaa tatataaggt
    51541 ttatacggtt actgtagcga atactcaaac cgctacttta tttcctatta tccgcgaaca
    51601 agtgaaacct gacagcattg tttatacgga ttgttatcgt agctatgatg tattggatgc
    51661 aggcgaattt agccatttcc gtatcaatca cagcacacat tttgccgaac gacaaaacca
    51721 tattaatgga attgggaact tttggaaccg ggcaaaacgt catttacgca agtttgacgg
    51781 cattcccaaa gagcattttg agccgtattt aaaggagtgc gaacggcgtt ttaacaacag
    51841 tgagataaaa gttcttgttc cattttaaaa caattagtaa aatcgagttt atcctagttg
    51901 tccaggacgg cctcatctct ttatagatag ctgcttacca agtatcctta attgctaatt
    51961 caagccactc ttcaaaacta ttaaaaaatt catggttttc tatattatct aaattaataa
    52021 caataaccct aggatttcct gttacatcat taccatcaaa ataagcttcc cattctccat
    52081 taccagcgaa tgggataaga tttaaatcag gcaggatgga atttctaaat atataagcta
    52141 cttctgcccc ttctgttcca taattctcaa accaccagtg aaaattatgt ggctgaatat
    52201 aaggtattaa agatgtatcg ttagagatac gaacatactt ttcaggataa caaaaccctt
    52261 caggaagcat attactagga tataaattaa attcattttg aataatcatt tcgttacctt
    52321 agtatcataa atactaccat taggaaactg ggtagaccaa tttggtggtg ctagtccatt
    52381 ttcactttgt ttttttaatt ctaaattgaa tagcttctcc ccatgtttgc ggcatctaat
    52441 gctgcttgct ctgcatcaat attactaaaa attagattag tttcaaaaat gaaaaattac
    52501 tgttttttca ttttttatag catattcaag tacatgaata atatttttca ctaagggctc
    52561 tgtagaaaaa tctgaatttt gtaatttttc ttttacagcc aaaatacttt ccttatctat
    52621 tagccaatcc ccctcacttg gtccaaataa ccaattatcg gtaatttcag aaacaagccc
    52681 ccataatcca cgattttgat attcaataat ttgattttcc gtaatttcta caactgtttc
    52741 agattgaatg ttttcataag cccaatcaac catttcagta tattcactaa ttgaaacatt
    52801 gtcttttaaa cagaaaatta acaggttgtg ttcattcatt tgtctaagtt cctgatatga
    52861 gtttgttgtt gtaaataatc ttttgcttgc ttaccttgta aattaccggt tttcacaaca
    52921 ttaccatttg aatcaagata ctgttttcgt gaattatcat ggattctaaa atagttgttt
    52981 tcgttatctt ttataattgt aatttttcca tctaaactag tgtatcgagt tttaacttta
    53041 tcaggactta aaataccagg tgcattggga gttagtgttt tgtgcactga atcaaaactt
    53101 gctgaactcc agttgctact aaacttctta aaactttgtt ttcttttttc agcggcgctg
    53161 tttggtaaag atttaatagg tttattccca cctgccattt ccccgtcaac agtctgcata
    53221 ggtcgggcag caggtttttc atagccggta tttttaaccc aatcccattt ggtcgggtta
    53281 agttctactt ttttaccgcg ccaaaccgta cctgcggcct ctgctacggc aagggcagtt
    53341 tgggctgttg ctgttatatt cggatgggca tcagcccatt gtctggcgga attaatgccg
    53401 tcttttaccg caaaggcact gtcctgtaat aggctcgcgg cagcaagttg tgcttccgga
    53461 cttaaatttc ctaaatcatt aataccttgt agagtctgct gagcggctgt atctgtgacc
    53521 gggcttaccg cactgtctgt aattgcccca atccctactc cttgaaaacc cgttaaaaaa
    53581 ggattaaccg caccttggac gatgccgcga atatcatcca tacggttagc ccgccaattt
    53641 ttatcggggt cgtcttccca tatcagtttt cctgcttcat ccgcacagtt aggattggca
    53701 ttgatgccca cccagtttca ggcagcctga aaaggatttt taatatacgg caaaaggtgg
    53761 gcaacgagtg cccaccctat ctagctaaca tttatcccaa gtatcatata tttttttaag
    53821 ttctttatcc aaaagaagaa ctttattttt ttcaacacca tgaattatct caaaatctct
    53881 acaatccacg catacgcctg aataaatttc tcctacaatt gcttgaataa tatacaactc
    53941 atctttatcg agactaattt taactaaatt cccttcaaaa tcaattaatt tcatgaataa
    54001 ttccttttat tttaatttgt catcaaaata ctgtttacct gtttttggtc taaatgctgt
    54061 cccaccatct ttagtcttgg atatttagat tataatcgtc aaattcccag tcaaaatatt
    54121 ttgccaaatt tgatatagac ttatcttcag acgttgaatt acatatttcc aatatatcat
    54181 ttatcaattt taatatgtgt tcaatattaa aacggtttgt aacaattgag ttttttaatg
    54241 acatcggtct atgtatattt gaaagtaacc attcggtgct atatatataa acataaaaat
    54301 aatcagcacc attactttca tcaaaaccta ttgataatgt caaaagtata caaaaatttt
    54361 ctaacttgtc ttgtgggaaa tcttccaaat caaagtcaga acaatcataa ttttttaatt
    54421 ttaatctcat ataattacct aatatttaaa tgtccatttt taattttatt tcttttttca
    54481 tttgaatcaa tagtataagt ttcaaaattt gcttgaatac ctgtagttgc aaattgtgat
    54541 tttttttctg ttggtggccg atattgtcga gtgccatctc tacttaacca tccgcctcca
    54601 ctagtttgtc ttgcaccctc accaacccaa attttaccta gtctatctgc ctcttcataa
    54661 gttgcagttc ctattggaaa atttttttta ccctcatgaa tagctagact caatcttgga
    54721 tcttgagccg caatgttatt taagttttgc tcatttagct gattaaccaa cttaggatag
    54781 gttgcagcat tagcttttcc ttccgacgtt atagatttag gcggtctatt cccccctgcc
    54841 atctccccat ctacagtctg catatggcgg gcagcaggtt ttttatagcc ggtattttta
    54901 acccaatccc atttggccgg gttaagttct acttttttac cgccccaaac cgtacctgc
//
LOCUS       DBJGGI010000006        53044 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.006, whole genome shotgun sequence.
ACCESSION   DBJGGI010000006 DBJGGI010000000
VERSION     DBJGGI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 53044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53044
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.006"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(263..1198)
                     /gene="hemC"
                     /locus_tag="ACSIGJ_000360"
     CDS             complement(263..1198)
                     /gene="hemC"
                     /locus_tag="ACSIGJ_000360"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HHQ1329333.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDRILDRTLSKVGGKGLFVKELEQSLQDGRADLAVHSIKDVPMDLPEGFALAAISERA
                     NPFDAFVSNRYARLEEMPEGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDERIRMILSESDSLPAAGQGALGIEIATHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLILRGLVGHPDGSIVLQADA
                     QAPAGYADALGRAVAKKLADDGAQELIGAVLNTEN"
     gene            1317..1832
                     /gene="ybeY"
                     /locus_tag="ACSIGJ_000361"
     CDS             1317..1832
                     /gene="ybeY"
                     /locus_tag="ACSIGJ_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HHQ1329334.1"
                     /translation="MKRAKKYPFLTLQRQRFHLNFENVSSAAGIPAERDFYRWAWSAL
                     KNEYLRADIGLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSERLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGTLHLMGYDHIKDDEAEIMEAEEIRLMRAA
                     GYPNPYREDGH"
     gene            1834..2658
                     /locus_tag="ACSIGJ_000362"
     CDS             1834..2658
                     /locus_tag="ACSIGJ_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HHQ1329335.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLTRLEKVLDFAELEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSVLRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGDIEDEFDEDESADNIHSVSAERWRIHAATEIEDINAFF
                     GTEYGSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(2801..4180)
                     /gene="nhaC"
                     /locus_tag="ACSIGJ_000363"
     CDS             complement(2801..4180)
                     /gene="nhaC"
                     /locus_tag="ACSIGJ_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HHQ1329336.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQAGMIGALNQGMGAVYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTACATVGVAFMGMAAAFQADMAMTAGAIV
                     SGVFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVVLALMRVNAVVAMLFTVIAAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDIAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAVRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(4470..5693)
                     /locus_tag="ACSIGJ_000364"
     CDS             complement(4470..5693)
                     /locus_tag="ACSIGJ_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HHQ1329337.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTGVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVIQGWAADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
     gene            6130..6543
                     /locus_tag="ACSIGJ_000365"
     CDS             6130..6543
                     /locus_tag="ACSIGJ_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HHQ1329338.1"
                     /translation="MIPSYTYAALAFSAFTSATLLPGTSEAAFALFVRNFPKHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLIRRFGIWLLAFAWLPVVGDALPLT
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            6590..7219
                     /gene="nth"
                     /locus_tag="ACSIGJ_000366"
     CDS             6590..7219
                     /gene="nth"
                     /locus_tag="ACSIGJ_000366"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HHQ1329339.1"
                     /translation="MNRQIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKS"
     gene            7357..8856
                     /locus_tag="ACSIGJ_000367"
     CDS             7357..8856
                     /locus_tag="ACSIGJ_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HHQ1329340.1"
                     /translation="MFKKYQYFALAALCAALLAGCEKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFAQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKNGYILTNTHVVAGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKNLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKASGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKAKLGN
                     AAEHTGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGKHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            9059..10579
                     /locus_tag="ACSIGJ_000368"
     CDS             9059..10579
                     /locus_tag="ACSIGJ_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HHQ1329341.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWADGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TASPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAASDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTFGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFSTTGIVLAVLIFLLKDKKRADV"
     gene            10636..11721
                     /gene="nagZ"
                     /locus_tag="ACSIGJ_000369"
     CDS             10636..11721
                     /gene="nagZ"
                     /locus_tag="ACSIGJ_000369"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HHQ1329342.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAIGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCAVIGNRSFHRNPEAVARLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLDELEAADLAPFRTMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELRDGFTIPDNQDLAGRWQYMENSLGHEAVQAVIQTTG
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            11802..11878
                     /locus_tag="ACSIGJ_000370"
     tRNA            11802..11878
                     /locus_tag="ACSIGJ_000370"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11836..11838,aa:Pro,seq:cgg)
     gene            complement(12214..12852)
                     /locus_tag="ACSIGJ_000371"
     CDS             complement(12214..12852)
                     /locus_tag="ACSIGJ_000371"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HHQ1329343.1"
                     /translation="MKKISVAPENPQYRIVEIFESLQGEGRNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYPARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CLETNGLKPAPPQIDYVATSPKACYAAKYETNCIAEADEVRIVADGDVLAFCENMERK
                     IRARHYYLSPCEQNGVMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(12849..13217)
                     /locus_tag="ACSIGJ_000372"
     CDS             complement(12849..13217)
                     /locus_tag="ACSIGJ_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HHQ1329344.1"
                     /translation="MKLLSTILVLLVAAEHFYIAWLEMTQIPGEKAAEMFKLPYEFME
                     QKRVQTLFGNQGLYNGFLAVGLLWAQFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAVLAV"
     gene            complement(13488..13922)
                     /gene="queD"
                     /locus_tag="ACSIGJ_000373"
     CDS             complement(13488..13922)
                     /gene="queD"
                     /locus_tag="ACSIGJ_000373"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HHQ1329345.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGTIKGG
                     AKDGMVMDFTDLKAIVKQHITDHFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYCRLKNAGLNVCRVKLWETPTSCAEYEGGEGIF"
     gene            complement(14010..14525)
                     /locus_tag="ACSIGJ_000374"
     CDS             complement(14010..14525)
                     /locus_tag="ACSIGJ_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HHQ1329346.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DTDKIHIDGWQVVEWADGYDIAVSETPKTKMPPENAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDYVRQHKDNLKDVDNLLALEHIGNFHIRLTPNPHGKP
                     AEIGFQGYLPI"
     gene            complement(14553..15212)
                     /gene="queC"
                     /locus_tag="ACSIGJ_000375"
     CDS             complement(14553..15212)
                     /gene="queC"
                     /locus_tag="ACSIGJ_000375"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HHQ1329347.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYGRENVQAITFRYGQRHA
                     VELERAEWIAQDLGVSQTVLDLSLMRQITHNALMDETAAIETADNGVPNTFVDGRNAL
                     FLLYAAIFAKGQGIRHIIAGVCETDFSGYPDCRGVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYKGIVGGCRECPSCILRERGLAECLESKKA
                     V"
     gene            complement(15416..15602)
                     /locus_tag="ACSIGJ_000376"
                     /pseudo
     CDS             complement(15416..15602)
                     /locus_tag="ACSIGJ_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779592.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
     gene            15701..16927
                     /locus_tag="ACSIGJ_000377"
     CDS             15701..16927
                     /locus_tag="ACSIGJ_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HHQ1329348.1"
                     /translation="MTFLQRWQGLADNKICAFAWFVIRRFSEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYIDAFRDQANRLTA
                     IGSVMLVVTSLMLIRTIDNAFNRIWRVNTQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDSVLSSGAQQWADALKTAARLAFMTLLLWGLYRFVPNRFVPARQAFVGALITA
                     FCLETARFLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGRTLSVQEFRRHINMGYDELGELLEKLA
                     RYGYIYSGRQGWVLKTGADSIELSELFKLFVYRPLPVERDHVNQAVDAVMTPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(16948..17229)
                     /locus_tag="ACSIGJ_000378"
     CDS             complement(16948..17229)
                     /locus_tag="ACSIGJ_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HHQ1329349.1"
                     /translation="MIREEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDEAASHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            17308..17424
                     /locus_tag="ACSIGJ_000379"
                     /pseudo
     CDS             17308..17424
                     /locus_tag="ACSIGJ_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(17425..19143)
                     /locus_tag="ACSIGJ_000380"
                     /pseudo
     CDS             complement(17425..19143)
                     /locus_tag="ACSIGJ_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
     gene            complement(19245..19460)
                     /locus_tag="ACSIGJ_000381"
     CDS             complement(19245..19460)
                     /locus_tag="ACSIGJ_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329350.1"
                     /translation="MDFAHYLKHWKAAVLIYLAISILTDILCYFLNFDGVFYKGRFFS
                     VTVAGPVGALSFLAYLLYLKREENRSH"
     gene            complement(19474..19821)
                     /locus_tag="ACSIGJ_000382"
     CDS             complement(19474..19821)
                     /locus_tag="ACSIGJ_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329351.1"
                     /translation="MRELTMNEMTAVFGGTEGTWGHRGRVSGEWVGSKVGGALGSFAG
                     AFTGMAGGALVTGGNPAGIAIGGAGGGWVGAEGGSALGGHIGGFAGAKWGKMIDRRNC
                     QCGEDCHCDPCNC"
     gene            complement(20006..21280)
                     /locus_tag="ACSIGJ_000383"
     CDS             complement(20006..21280)
                     /locus_tag="ACSIGJ_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HHQ1329352.1"
                     /translation="MNRPKQPFFRPEVAIARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTMEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQKLH
                     ISQQIDGQKRRIRLAEEMLRKYRFLSANDAVSKQEMMNVEAELLEQKAKLDAYRREEA
                     GLLQEIRTQNLTLASLPKRHETEQSQLERTIADISQEVLDFEMRSEQIIRAGRSGYIA
                     IPNVEVGRQVDPSKLFLSIVPERTELYAHLYIPSSAAGFIKPKDKVVLRYQAYPYQKF
                     GLASGSVVSVAKTALGRQELSGLGMVSSDLAKSNEPVYLVKIKPDKPTITAYGEEKPL
                     QIGMTLEADILHEKRRLYEWVLEPIYSMSGRL"
     gene            complement(21341..21613)
                     /locus_tag="ACSIGJ_000384"
     CDS             complement(21341..21613)
                     /locus_tag="ACSIGJ_000384"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329353.1"
                     /translation="MLPQPKEVPHGGILLQYLLLQRQFISCRKIFSVLYGANGVYNLT
                     RDWVNDAVNAPPYNGRPIFEIEHGLTAPATKADKSGNGYTDGTDYC"
     gene            complement(21688..21915)
                     /locus_tag="ACSIGJ_000385"
     CDS             complement(21688..21915)
                     /locus_tag="ACSIGJ_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329354.1"
                     /translation="MKNWKQFIFFVILVIACYQLLYSLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQEKEYKND"
     gene            complement(21924..23078)
                     /locus_tag="ACSIGJ_000386"
     CDS             complement(21924..23078)
                     /locus_tag="ACSIGJ_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JmjC domain-containing protein"
                     /protein_id="HHQ1329355.1"
                     /translation="MGIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLEAASCKYINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDARDMYAVQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHFA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQAKHEEVRKLIYQLIELDFLEIL"
     gene            23330..23611
                     /locus_tag="ACSIGJ_000387"
     CDS             23330..23611
                     /locus_tag="ACSIGJ_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329356.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPAGFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQRSDGIPANHTKEKP"
     gene            23608..24651
                     /gene="argC"
                     /locus_tag="ACSIGJ_000388"
     CDS             23608..24651
                     /gene="argC"
                     /locus_tag="ACSIGJ_000388"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HHQ1329357.1"
                     /translation="MSKKIKAGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEAGDNFKAYGTAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGSDPETVLRDYYRDSPFMDI
                     LPAGSTPETRSVRGANLCRISIRQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     EETHGLGGIPLLP"
     gene            complement(25245..27287)
                     /gene="recG"
                     /locus_tag="ACSIGJ_000389"
     CDS             complement(25245..27287)
                     /gene="recG"
                     /locus_tag="ACSIGJ_000389"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HHQ1329358.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLGRLKLPRLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRVSGTAAALRGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTHPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVCLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLSIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPEPNIG
                     LVHGRMKAAEKAEVMAEFAAGRLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAGESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(27373..27816)
                     /gene="secB"
                     /locus_tag="ACSIGJ_000390"
     CDS             complement(27373..27816)
                     /gene="secB"
                     /locus_tag="ACSIGJ_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HHQ1329359.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGDPEVDMRVST
                     GSQKLEDGYYDVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(27837..28094)
                     /gene="grxC"
                     /locus_tag="ACSIGJ_000391"
     CDS             complement(27837..28094)
                     /gene="grxC"
                     /locus_tag="ACSIGJ_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HHQ1329360.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQRLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            28313..29806
                     /gene="rng"
                     /locus_tag="ACSIGJ_000392"
     CDS             28313..29806
                     /gene="rng"
                     /locus_tag="ACSIGJ_000392"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HHQ1329361.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSGHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNIEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(29841..31091)
                     /locus_tag="ACSIGJ_000393"
     CDS             complement(29841..31091)
                     /locus_tag="ACSIGJ_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHQ1329362.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVGPDSKSET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLRADGDDDYYRAYIRTTEQGRIAV
                     MQENEYREDLAEDAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRING
                     LPALSVYNIPSEIRGFVTAINLLLKRVDEDIRRRQRFVADAAHELRTPMTALSLQAER
                     LNNMPLPPDAGRQSAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFRQV
                     LQELMPLVLEKRQDIGVAVGGDVEVSADETEIYTLVKTFADNAVRYTPNGGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESECARVLDPFYRILGTEQQGAGLGLSIADTLAKKYGGYL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(31173..31802)
                     /locus_tag="ACSIGJ_000394"
     CDS             complement(31173..31802)
                     /locus_tag="ACSIGJ_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHQ1329363.1"
                     /translation="MSASLKDGGYAVDWVKNGAQVAAAAAAQPYDLMLLDLGLPGRDG
                     LDVLSEIRAAGCTVPVLIVTARDDLYSRLNGLDGGADDYIVKPFDMAEFKARMRAVLR
                     RGSGQAQACLSNGALSLNPATYQVEIIAEGRQVALSNQEFSVLQALLARPGVILSRSD
                     SEDKVYGWGGEVESNAVDFLIHGLCKKLGKESIQNVRGVGWLMPRQDAV"
     gene            complement(32070..33383)
                     /locus_tag="ACSIGJ_000395"
     CDS             complement(32070..33383)
                     /locus_tag="ACSIGJ_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0046942 - carboxylic acid transport
                     [Evidence IEA];
                     GO_process: GO:1905039 - carboxylic acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HHQ1329364.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLIGALLAGFDVS
                     QLKEFYSGGTKSVTQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLSVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPRGGPI
                     GRVASVLGADVGELYKPLLTVQIIGVVFILVLSLFLGVREKRRIVRELGALPAVADLI
                     KPAPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDLVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILISSIAAGIVPLP"
     gene            complement(33761..34333)
                     /locus_tag="ACSIGJ_000396"
     CDS             complement(33761..34333)
                     /locus_tag="ACSIGJ_000396"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HHQ1329365.1"
                     /translation="MIVAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGH
                     LPLYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGS
                     KPNADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLF
                     DENGKLIDSARDFVQSYINQFVGLIERNAK"
     gene            34492..35901
                     /gene="dacB"
                     /locus_tag="ACSIGJ_000397"
     CDS             34492..35901
                     /gene="dacB"
                     /locus_tag="ACSIGJ_000397"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine endopeptidase"
                     /protein_id="HHQ1329366.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRAGIPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLRDKGIRNITGRLMLDHSLWGEVGSPDHFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAAGSTDILTDPPLPHIFAQNNLKITASQAACPSVKKLMRASF
                     SGNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNRWLLGGGRISDGIGIADTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVAKNI
                     ISGGDGWLDAKLMCKERRA"
     gene            complement(35966..37135)
                     /gene="metK"
                     /locus_tag="ACSIGJ_000398"
     CDS             complement(35966..37135)
                     /gene="metK"
                     /locus_tag="ACSIGJ_000398"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HHQ1329367.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPAISHEELSKAVIEQIIKPVLPP
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLIALV
                     CEHFDLRPKGIVQMLDLLRPIYGKSAAYGHFGREEPEFTWERTDKAASLKAAAGL"
     regulatory      complement(37319..37428)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            37481..38374
                     /locus_tag="ACSIGJ_000399"
     CDS             37481..38374
                     /locus_tag="ACSIGJ_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HHQ1329368.1"
                     /translation="MFRLQFRLFPPLRTAMHILLTALLKCLSLLSLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGLNPDTQTVKAVFAETAKCGLELAPAFFKKPEDIETMF
                     KAVHGWEHVQQALDKGEGLLFITPHIGSYDLGGRYISQQLPFHLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTGIQGVKQIIKALRAGEATIILPDHVPSPQEGGGVWADFFGKP
                     AYTMTLAAKLAHVKGVKTLFFCCERLPDGQGFVLHIRPVQGELNGNKAHDAAVFNRNT
                     EYWIRRFPTQYLFMYNRYKTP"
     gene            complement(38419..39483)
                     /gene="tsaD"
                     /locus_tag="ACSIGJ_000400"
     CDS             complement(38419..39483)
                     /gene="tsaD"
                     /locus_tag="ACSIGJ_000400"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HHQ1329369.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRSHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYELLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVREANGSETIPEQTRNNICRAFQDAVVEVLEAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPMAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWSLSEIVK"
     gene            complement(39599..40786)
                     /gene="ccsB"
                     /locus_tag="ACSIGJ_000401"
     CDS             complement(39599..40786)
                     /gene="ccsB"
                     /locus_tag="ACSIGJ_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HHQ1329370.1"
                     /translation="MTEHYKTLPEHELLIQKSLIRNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAVQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEEKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(40779..42731)
                     /locus_tag="ACSIGJ_000402"
     CDS             complement(40779..42731)
                     /locus_tag="ACSIGJ_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HHQ1329371.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWTRIFDF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVRGFQGKTVSREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLAGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGMLVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLRDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPILQDKDYFWLTGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKGQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLSEWRQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVFMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(43009..43632)
                     /locus_tag="ACSIGJ_000403"
     CDS             complement(43009..43632)
                     /locus_tag="ACSIGJ_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HHQ1329372.1"
                     /translation="MRRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            43839..44468
                     /gene="yihA"
                     /locus_tag="ACSIGJ_000404"
     CDS             43839..44468
                     /gene="yihA"
                     /locus_tag="ACSIGJ_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HHQ1329373.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLRH
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(44589..46985)
                     /locus_tag="ACSIGJ_000405"
     CDS             complement(44589..46985)
                     /locus_tag="ACSIGJ_000405"
                     /EC_number="2.4.99.28"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HHQ1329374.1"
                     /translation="MIKKILTTCFGLFFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRRELYE
                     KYGEDAYTQGFKVYTTVRTDHQKAATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVALDRRALGFAARAVDNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRPSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRAGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLM
                     LDNSGIAPQPSRRAKEDDEAAVENEQQGRSDETRQDVQETPVLPSNTDSKQQQLDSLF
                     "
     gene            47137..48252
                     /gene="pilM"
                     /locus_tag="ACSIGJ_000406"
     CDS             47137..48252
                     /gene="pilM"
                     /locus_tag="ACSIGJ_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HHQ1329375.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIVASVPQ
                     NLATIEQLTYTAKDAELDLQGFVESSISEASSISLEEANYDYQVLSQSAVGEAVLSVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELADEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTAEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAVRQKGIAQTVASQTNADVQ
                     CVHPARYFANDLKTDEQQFELDAPTLTKAFGLAVRGL"
     gene            48255..48854
                     /locus_tag="ACSIGJ_000407"
     CDS             48255..48854
                     /locus_tag="ACSIGJ_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HHQ1329376.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINKQSEKNTLLEISIAHLDTELSKIQKLKQEKDAFLIKKNKIGELQIKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            48855..49502
                     /locus_tag="ACSIGJ_000408"
     CDS             48855..49502
                     /locus_tag="ACSIGJ_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HHQ1329377.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLVVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKKLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            49520..50065
                     /locus_tag="ACSIGJ_000409"
     CDS             49520..50065
                     /locus_tag="ACSIGJ_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HHQ1329378.1"
                     /translation="MKHYALLISFLALSACSQSSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETAKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIILNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            50084..52279
                     /gene="pilQ"
                     /locus_tag="ACSIGJ_000410"
     CDS             50084..52279
                     /gene="pilQ"
                     /locus_tag="ACSIGJ_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HHQ1329379.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPFTESVV
                     SVSAPFSPAKQQAAASAKQQAATPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQ
                     HDHIIVTLKNHTLPTALQRSLDVADFKTPVQKVTLKRLNNDTQLIITTTGNWELVNKS
                     AAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNI
                     VASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAFLQ
                     AEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATN
                     TLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGRKKLK
                     NETSAFGWGVNSGFGGGDKWEAQTKINLPVAAAANSISLVRAISSGALNLELSASESL
                     SKTKTLANPRVLTQNRKEAKIESGYEIPFTVTTASGGGNSTNTELKKAVLGLTVTPNI
                     TPDGQIIMTVKINKDSPAQCASGNNTILCISTKSLNTQAMVENGGTLIVGGIYEENNG
                     NTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFITPRIIDTAGNSLRY"
BASE COUNT        13236 a        14937 c        12949 g        11922 t
ORIGIN      
        1 aaaaaatacg aatatcatca ttctcaaatt caaaatttga acgccacagt attatccgaa
       61 gccacaaaaa cctttccgcc gtcattcccg cgcaggcggg aatctggaaa cccaatgctg
      121 caagaattga ttaaaaacaa ctgaaaccga gcaaactgga ttcccgcctg cgcgggaatg
      181 acgaaataag gtaagtagaa cttattaaat aaattaatta aaaacaataa cttatattat
      241 tttctttaaa ttttcgataa aatcaatttt ccgtattcaa tactgctcca atcaattcct
      301 gcgcaccgtc gtccgccagt tttttggcaa ccgcacgtcc aagcgcatcg gcatatccgg
      361 caggggcttg cgcgtccgcc tgcaacacaa tcgatccgtc ggggtgcccg accaatccgc
      421 gcaagatcag cagcccgttt tcttcagtgc aatacgcggc cagcggcact tggcagcttc
      481 cgcccaaagc gcgcgccaaa gcgcgttcgg cggtaacgca ggcgtgtgtg gtatcgtggt
      541 ttaacggctt caagacttcg tacaaatctt cgcgatgcgt ggcaatttca atacccaatg
      601 cgccttgtcc ggcggcaggc aggctgtcgg attccgacaa aatcatgcgg atgcgttcat
      661 ccaattccag acgctgcaac ccggcggcag ccaagataat tgcatcgtat tcgccgttgt
      721 cgagtttgga caaacgggtt tgcacattgc cgcgcaaagg tttgataagc aaatgcggat
      781 agcgcgcgcg caactgggct tcacggcgca ggctggatgt gccgacaacc gcgccttcgg
      841 gcatttcttc caaacgcgcg tatcggttgg acacaaacgc gtcaaacgga ttggcgcgtt
      901 cgctgatggc ggcaagggcg aagccttcgg gcaaatccat cggcacgtcc ttaatcgaat
      961 gcaccgccaa atcggcgcgc ccgtcttgaa gggattgttc caactctttg acaaacaagc
     1021 ctttcccgcc gacttttgac aaagttctgt ccaaaatccg gtcgccgcgc gtggtcatgc
     1081 ccaaaatttc gacttcgcaa tcgggataca gcgccttcag acggccttgg atatgtttcg
     1141 cctgccacat ggcaagcagg ctttcgcggc tggcgataac aagttttttc gggttcatgt
     1201 tcaatgcgtt caaagaatgg aaacggcgtg gagtctatca tattttgaac gtttcttcag
     1261 acggcaccgt caggtctatg ttatatttac gcccacaaaa cacgaaaccc gccacaatga
     1321 aacgcgccaa aaaataccct ttcttaacgt tgcagcggca acgtttccat ttgaactttg
     1381 aaaacgtctc ttccgccgcc ggcatccccg ccgaacgcga tttttaccgc tgggcgtggt
     1441 ctgccttgaa aaacgaatac ctccgcgccg acatcggttt gattcttttg gacgaagaag
     1501 aagcccgcgc ctacaaccgc gactaccggg gcaaagatta tgccaccaat gtattgagtt
     1561 tcgcgctcaa cgaaggcgaa atcctgccct gccaagtttc ggaaagactg tacggcgatt
     1621 tgattatctg cccgcaagtc gttttaaaag aagccgccga acagggcaaa acgcctgaac
     1681 ggcattttgc ccacttaacc atacacggca cgctgcacct gatgggatac gaccacatca
     1741 aagacgatga ggccgaaata atggaagccg aagaaatccg cctgatgcgg gcggcaggct
     1801 atcccaaccc ctaccgagag gacggacatt aaaatggacg gcgcacaacc gaaaacaaat
     1861 ttttttgaac gcctgattgc ccgactcgcc cgcgaacccg attccgccga agacgtatta
     1921 aacctgcttc ggcaggcgca cgaacaggaa gtttttgatg ccgacacact gacccggctg
     1981 gaaaaagtat tggactttgc cgagctggaa gtgcgcgatg cgatgattac gcgcagccgc
     2041 atgaacgtat tgaaagaaaa cgacagcatc gaacgcatca ccgcctacgt catcgatacc
     2101 gcccattcgc gcttccccgt catcggcgaa gacaaagacg aagttttggg cattttgcac
     2161 gccaaagacc tgctcaaata tatgttcaac cccgagcagt tccacctgaa atccgtcttg
     2221 cgccctgccg ttttcgtgcc cgaaggcaaa tctttgaccg cccttttaaa agagttccgc
     2281 gaacagcgca accatatggc aatcgtcatc gacgaatacg gcggcacgtc gggtttggtc
     2341 acctttgaag acatcatcga gcaaatcgtc ggtgacatcg aagacgagtt tgacgaagac
     2401 gaaagcgccg acaacatcca ctccgtttcc gccgaacgct ggcgcatcca cgcggctacc
     2461 gaaatcgaag acatcaacgc ctttttcggt acggaatacg gcagcgaaga agccgacacc
     2521 atcggcggct tggtcattca ggaattggga cacctgcccg tgcgcggcga aaaagtcctt
     2581 atcggcggtt tgcagttcac cgtcgcccgc gccgacaacc gccgcctgca cacgctgatg
     2641 gcgacccgcg tgaagtaagc agagcctgcc cgcaccgccg tttctgcaca gtttaggatg
     2701 acggtacggt cgttttctgt ttcaatccgc cccatccgcc aaacataaaa atgccgtctg
     2761 aaaccggaaa acaggttcag acggcatttc gcttatccgc ttattttttg ctcaaagtca
     2821 gccccgtcca gccgaataac agggttaaag ccaaactcaa atagcagaaa aaggcataag
     2881 gcagatattc ccaaacgggt acgccaaggg cgtggctgat aaatacgccg cacacgctcc
     2941 acggcacgag cgggttaatc accgtccccg catcttccag agtccgcgac aggttgcgcg
     3001 aatgcaggcc gagtttgtcg taaacgggtt tgaacgtttc tcccgaaagc aggatgctca
     3061 aatattgctc tccaatcagg aaattgaccc cgaccgaagt catggcaacg ctgaacgtcg
     3121 cgcgtccggc attcgtcaag aaggtacgga cggcctccag cagggaagga atcacaccga
     3181 gcgcaaacag cagcccgccc aaactcatac cgaggataac gatggtctgc gtaaagaaca
     3241 tactctccaa gccgccgcgc gaaatcagtt tggcaatgtc tttaaacgct tcgccttcga
     3301 gtttgtagcc gccataaaac cacgcgccga gctgacgcag atcgggcgtg ctgtgcagat
     3361 acgttacggc aacggcggca atgacggtaa agagcatggc gaccacggca ttgacgcgca
     3421 tcaatgccaa aacgaccaac agtgcaaacg gaatcagcga atagccgtgc accaatcccg
     3481 tggcttcaag ctggctgcgg aaggattcga cgctgttcaa atcctgcgcg gcgacgctgg
     3541 gaagaagcca aagcatcagt gccgcgctga taagccacgc ggggatggtg gtgtacatca
     3601 tgtttttgat gtgttcaaac aggtcgatac cgacgatgga cgcggaaatg cccgtggtgt
     3661 cggaaagcgg ggacatttta tcgccgaaaa acacaccgga aacaatcgcg cccgccgtca
     3721 tcgccatatc ggcctgaaac gccgccgcca tccccataaa ggcaacgccg acagtggcgc
     3781 aggcggtcag gctgctgccg atggacacgc cgatgacgga acacagcgcg aaggcggaaa
     3841 aataaaaata agtcggggaa atcagcccga aaccgtaata catcagcgtc ggaatcgcgc
     3901 cgctcatcat cagcgcgctg accatcagcc cgatgaagaa aaacaggtaa accgcgccca
     3961 taccctgatt caacgcgcct atcatccctg cctgcatatc gttgtatttc aaaccgcgcg
     4021 ccaagccgta caaaatcagc acgacgatgg cggcaataat ggacatatgc ggcagccact
     4081 ccaatgaaat gatggtatag cccattgcgg caatcagagc gacgacgacg gcaagtgcct
     4141 caccgcgcgg catatcgagt aaggatttga aagcaaacat gattctttgc ccgatttgat
     4201 ataaagttaa cggattctaa cacaccatgc cgaatctgcc cgacgttttc agacggcatt
     4261 ttagcgtttt acaaaagccg ggcagaaaca aaacggcaat tttctcgatt taaattaatt
     4321 tttttgaaat ataattttta tttttttatt tgttggcata taagactttt atggattgaa
     4381 gtattaaaac ctcggcacac acgccgcaaa caccggtacg caaaaatgcc gtctgaaaga
     4441 tgttcagacg gcatttctca agaaaaagat tatttgtccg ccctatatcc gtacaccgca
     4501 aaaaacacga tatacaggta acagataacg gaaacgacaa acgaagacat cagggtgtaa
     4561 gtatccgccg cccaaccttg aattaccggc acgaccgcac cgccgacaat cgcggtacac
     4621 agcacaccgg aagccgcgtt ggtgaatttg cccaaccctt tggttgccaa agagaaaatc
     4681 gtcgggaaca taatcgagtt gaaaaaaccg atggcaagca gcgaccacat cgccacatcc
     4741 gcattgccgc tgcccgtcgc catcgcgacg gcaagcagca cgaccgcagc cgatgcgtta
     4801 aacgccaaat aacggttggg cgcgaatttc gccattaccg ccgaaccgag gaaacgtccg
     4861 accatcgctc cgccccaata aaacgacagg taatgcgcgg cagaagcatg atccagccct
     4921 ttcagataac ccaacacatt gaccatcagc gaaccgatag acacctccgc accgacgtag
     4981 cagaaaatgc ctgccgtacc gaacacgaga tgtttgtatt gccatacgcc ggttttgccg
     5041 tcgtggttgt gcgcgctttc ctcggcggca attttgcgcg cgtcgggcag ccggatcatt
     5101 ttcacgaaaa cggcaaggat aatcagcagc cccgccagtc ccaaataggg aatctgtacg
     5161 gaagaaatct gttccgcctt gctgacggtt tgggttgcgt ccgccaaaat caggaacgcg
     5221 ccgatttgag gcgcaatggt cgtacccaaa gcgttaaacg cctgaaccag cgtcagcgtt
     5281 gccgattcct tgccgggttt cgccagcagg gtaacataag gattaccggc gacctgaagc
     5341 agcgttacgc cggaagccaa aataaacaac gcgcccaaaa ataccgcgta agaatggctg
     5401 cccgcagcag gataaaacag caggcacccg accgccgtca gcagaaagcc gccgataacg
     5461 ccgtttttgt agccgacttt gccgacaaaa gcccccatcg ggatggacat taccgcatag
     5521 gcggtaaaaa agcaaaattg aatcagcatc gcctgaacgt aagagaggtc gaaaatttct
     5581 ttcaaatgag ggataaggat gtcgttcatg caggtaataa aacccatcat gaagaacagc
     5641 gtggtcaaga cgaccaatgg ggacgtatgg ttgttttgtg attgcgcaga catgatttgc
     5701 ctttcgtggt gtcggaatat tgcccgtttc aagaaaatag aaattgacat ttcttaacgg
     5761 caatgcaaat catactgata tttgaacatt ttgcaaactt tatcggatgc aggcaatgcg
     5821 ccgatctgaa attcaagcag cagaaattaa tattttacag ttgtttgcat aggttttaat
     5881 agattttaaa aaattccggc gggcttgcaa aaaccgttcc caataaaatg aaaatcctcc
     5941 gccgtatccg gaaaattcgg aaatgccgca tcggggcatc aaaaccggag cggaaagaat
     6001 gtcgtatgcc cgacaaataa aggggcatac ggctgtgccg tatttttgca ccgtcctccg
     6061 cccttcggcg ggggcgtgaa aataccgtat aatccgtcgc cgatttttac ctgtttggga
     6121 ctgtttgcaa tgataccctc ctacacctac gccgccctcg ctttttccgc cttcacttcc
     6181 gccaccctcc tgcccggcac gtccgaagcc gccttcgccc tgttcgtccg caacttcccc
     6241 aaacacgcat acggcgcgtt gctgtgcgcc ggccttgcca acggattggg cagtatggtt
     6301 tcctattgga tgggacgttt gctgccttcc cgaaaaatgc cgtctgaaaa aacactgaat
     6361 ctgatacggc gtttcggtat ttggctgctt gcgtttgcgt ggctgcccgt cgtcggcgac
     6421 gcactgccgc ttaccgccgg ctggctgcgg ttgaatccgt ggacaagcgg gctgatgctg
     6481 gttatcggca aaacggcgcg ttacgccttc attctgtggg gaatgcaata ttacgccgcc
     6541 tgaaccgtgc gttataatgc cgaccgcaca aaccgactgc acaaaaaaaa tgaacagaca
     6601 aatccgccaa gaaatcttcg agcgtttccg cgccgccaac ccccatccga ccaccgagct
     6661 gaatttcaac tcccctttcg agcttttaat cgccgttctg ctttcggcgc aggcgaccga
     6721 tgtcggcgta aacaaggcga cggcgaagct gtttccggtt gccgatacgc cgcaggcgat
     6781 gctggatttg ggcttggacg gcgtgatgga atacacgaaa accatcgggc tgtataaaac
     6841 caagtccaaa cacattatgc aaacctgccg catcctgctg gaaaaataca acggcgaagt
     6901 gccggaagac cgcgaggctt tggaatcgtt gcctggcgtg gggcgcaaaa cggcaaacgt
     6961 ggtattgaac acagcgttcg gacaccccgt tatggcggtc gatacgcata ttttccgcgt
     7021 gtccaaccga accaaaatcg cacccggaaa agatgtacgc gaagtcgaag acaaactgat
     7081 gcgcttcatt cctaaagaat ttctgatgga cgcgcaccac tggctgattt tgcacggacg
     7141 ctacacctgc aaggctttaa agccgcaatg ccaaacctgc atcatcaacg atttgtgcga
     7201 atatcccgcc aaatcctgac cgccgccgcc cttcaaaatg aaacgcttga aaattcggac
     7261 ggacgttgta gaatttcaac ctatgccgtc tgaagccgac aaaatgcgct tcagacggca
     7321 ttaaagccgg taccggcatc cgacaaagga caaaacgtgt tcaaaaaata ccaatacttc
     7381 gctttggcgg cactgtgtgc cgccttgctg gcaggctgcg aaaaggcagg cagctttttc
     7441 ggtgcggaca aaaaagaagc atccttcgta gaacgcatcg aacacaccaa agacgacggc
     7501 agtgtcagta tgctgctgcc cgactttgcc caactggttc aaagcgaagg cccggcagtc
     7561 gtcaatattc aggcagcccc cgccccgcgc acccaaaacg gcagcggcaa tgccgaaacc
     7621 gattccgacc cgcttgccga cagcgacccg ttctacgaat ttttcaaacg cctcgtcccg
     7681 aacatgcccg aaatccccca agaagaagca gatgacggcg gattgaactt cggttcgggc
     7741 ttcatcatca gcaaaaacgg ctacatcctg accaataccc acgtcgttgc cggtatgggc
     7801 agtatcaaag tcctgctcaa cgacaagcgc gaatataccg ccaaactcat cggttcggat
     7861 gtccaatccg atgtcgccct tctgaaaatc gacgcaacgg aagagctacc cgtcgtcaaa
     7921 atcggcaatc ccaaaaattt gaaaccgggc gaatgggtcg ctgccatcgg cgcgcccttc
     7981 ggctttgaca acagcgtgac cgccggcatc gtgtccgcca aaggcagaag cctgcccaac
     8041 gaaagctaca cacccttcat ccaaaccgac gttgccatca atccgggcaa ttccggcggc
     8101 ccgctgttca acttaaaagg acaggtcgtc ggcatcaatt cgcaaatata cagccgcagc
     8161 ggcggattca tgggcatctc ctttgccatc ccgattgacg ttgccatgaa tgtcgccgaa
     8221 cagctgaaaa acaccggcaa agtccaacgc ggacaactgg gcgtgattat tcaggaagta
     8281 tcctacggtt tggcacagtc gttcggtctg gataaagcca gcggcgcatt gattgccaaa
     8341 atccttcccg gcagccccgc agaacgtgcc ggcctgcagg cgggcgacat cgtcctcagc
     8401 ctcgacggcg gagaaatacg ttcttccggc gaccttcccg tcatggtcgg cgccattacg
     8461 ccgggaaaag aagtcagcct cggcgtatgg cgcaaaggcg aagaaatcac aatcaaagcc
     8521 aagctgggca acgccgccga gcataccggc gcatcatcca aaacagatga agccccctac
     8581 accgaacagc aatccggtac gttctcggtc gaatccgcag gcattaccct tcagacacat
     8641 accgacagca gcggcaaaca cctcgtcgtc gtacgggttt ccgacgcggc agaacgcgca
     8701 ggcttaaggc gcggcgacga aatcctcgcc gtcgggcaag tccccgtcaa tgacgaagcc
     8761 ggtttccgca aagctatgga caaggcaggc aaaaacgtcc ccctgctggt catgcgccgt
     8821 ggcaacacgc tgttcatcgc attaaacctg caataacgca cataatttaa ccaaaatatt
     8881 tttaaatgat aaaatgcccg tcaataccga ttaacgggca ttttacaaaa atcagttggt
     8941 tgattttaaa caaatatttt ttacgacaaa aaatatttcc acccattttg ccaacaaaac
     9001 ccaaaccgga ggcggacaga tacccaccca tccggacgct taacaccccc cataaaccat
     9061 gcagctgatt gactattcac attcattttt ctcggttgtg ccaccctttt tggcactggc
     9121 acttgccgtc attacccgcc gcgtactgct gtctttaggc atcggtattt tggtcggcgt
     9181 tgcctttttg gtcggcggca accccgtcga cggtctgaca cacctgaaag acatggtcgt
     9241 cggcttggct tgggcagacg gcgattggtc gctgggcaaa ccaaaaatct tggttttcct
     9301 gatacttttg ggcattttca cttcactgct gacctactcc ggcagcaatc aggcgtttgc
     9361 cgactgggca aaacggcaca ttaaaaaccg gcgcggcgcg aaaatgctga ccgcctgcct
     9421 cgtgttcgta acctttatcg acgactattt ccacagcctc gccgtcggtg cgattgcccg
     9481 ccccgttacc gacaagttta aagtttcccg cgccaaactc gcctacatcc tcgactccac
     9541 tgcctcgccc atgtgcgtgc tgatgcccgt ttcaagctgg ggcgcgtcga ttatcgccac
     9601 gcttgccgga ttgctcgtta cctacaaaat taccgaatac acgccgatgg ggacgtttgt
     9661 cgccatgagc ctgatgaact attacgcgct gtttgccctg attatggtat tcgtcgtcgc
     9721 atggttctcc ttcgacatcg gctcgatggc gcgtttcgaa caggctgcgt tgaacgaagc
     9781 ccacgacgaa accgccgctt cagacgctac caaaggtcgt gtttacgcat tgattattcc
     9841 cgttttggcc ttaatcgcct caacggtttc cgccatgatc tacaccggcg cgcaggcaag
     9901 cgaaaccttc agcattttgg gggcatttga aaataccgac gtaaacactt cgctggtatt
     9961 cggcggcact tgcggcgtgc ttgccgtcgt cctctgcacg ttcggcacga ttaaaaccgc
    10021 cgattatccc aaagccgtgt ggcagggtgc gaaatccatg ttcggcgcaa tcgccatttt
    10081 aatcctcgcc tggctcatca gtacggttgt cggcgaaatg cacacgggcg actacctctc
    10141 cacgctggtt gcgggcaaca tccatcccgg cttcctgccc gtcatcctct tcctgctcgc
    10201 cagcgtgatg gcgtttgcca caggcacaag ctgggggacg ttcggcatta tgctgccgat
    10261 tgccgccgcc atggcggtca aagtcgaacc cgcgctgatt atcccgtgta tgtccgcagt
    10321 aatggcgggg gcggtatgcg gcgaccactg ttcgcccatc tccgacacga ccatcctgtc
    10381 gtccaccggc gcgcgctgca accacatcga ccacgttacc tcgcaactgc cttatgccct
    10441 gacggttgcc gccgccgccg catcgggcta cctcgcattg ggtctgacaa aatccgcgct
    10501 gttgggcttt agcacgaccg gtattgtatt ggcggtgctg atttttctgt tgaaagataa
    10561 aaaacgcgcc gacgtttgac cttcccccct gttcagacgg catatgccgt ctgaagtttt
    10621 ttggagcaaa ccgcaatgac cgtcccccat attccgcgcg gccccgtcat ggcagacatt
    10681 gccgccttcc gcctgaccga agaagaaaaa caacgcctgc tcgatcccgc aatcggcggc
    10741 atcatcctct tccgccgcaa cttccaaaac atcgaacaac tcaaaacact caccgccgaa
    10801 atcaaagccc tgcgcacacc cgaactcatt atcgccgtcg atcacgaagg cggcagggtg
    10861 caacggttta tcgaaggctt cacccgcctg cctgcaatga acgttttagg gcaaatttgg
    10921 gacaaagacg gcgcgtccgc cgccgaaacc gctgccggac aagtcggccg ggttttggca
    10981 accgagcttt ccgcctgcgg catcgatttg tccttcacgc ccgtcttgga tttggactgg
    11041 ggaaactgcg ccgtcatcgg caaccgcagc ttccaccgca atcccgaagc ggtcgcccgc
    11101 ctcgccctcg cccttcaaaa aggtttggca aaaggcggta tgaaatcatg cggcaaacat
    11161 ttccccggac acggatttgt cgaaggggac agccatttgg tgctacccga agacgggcgc
    11221 agcctcgacg aactcgaagc cgccgacctc gcccccttcc gcactatgag ccgcgaaggt
    11281 atggcggcgg tcatgcccgc ccacgtcgtt tatccgcaag tggacacaaa gcccgcaggg
    11341 ttttccgaaa tctggctcaa acaaattttg cgccgcgata tcggattcaa aggcgtgatt
    11401 ttttctgacg atttgaccat ggagggcgcg tgcggggcag gcggcatcaa agaacgcgcc
    11461 cgcatttcct ttgaggcagg ttgcgacatc gtcctcgtct gcaaccgtcc cgacttagtc
    11521 gatgaattgc gcgacggttt caccattccc gacaaccagg atttggccgg acgttggcaa
    11581 tacatggaaa actcgctcgg tcatgaggcg gtacaagccg ttatacagac aacgggtttc
    11641 caagcggcgc aagcctttgt tgccggcctt gcctcgccgc aggacacggc gggcggcgta
    11701 aaagtcggag aggcctttta aatccgcccg acacgcggca aaaaggcaaa acggtatagg
    11761 cacgccgtat tttttgcgct ataatcacac cttaattaat tcggaatgta gcgcagcccg
    11821 gtagcgcact tcgttcggga cgaaggggtc ggaggttcga atcctctcat tccgaccata
    11881 ttcagaccgc ctcgacatcg attgccgggg cggtttttcc cttttggcgg caacaaaaaa
    11941 atgccgtctg aaacagaatg ctgtttcaga cggcatttcg ttttccccaa tcctgccatt
    12001 tgcgcgcact gacaaagggt atcggcaagg ttcaaaagac catcggaatc taggaagcct
    12061 ggcgtgtacc caaccgcggc atcttattca ttaaggcttc gtcctgattg ggcggcggaa
    12121 atgtcggcag tttcacgcgg aagcggcaca ctttccccgt atgtcaaaca tcggcaagaa
    12181 ggtacgcctg ccctttgggt tacactgctt aaactactct attcccgccc atttgtgcgt
    12241 ctgcacgctc aactgccaat gcacgggcgc gtcggggcgg ctgtttaaaa taccgatttg
    12301 gcggatggtg tcgtagatgt tcatcacgcc gttttgttcg cagggagaaa gatagtaatg
    12361 gcgcgcacgg attttgcgtt ccatattttc gcaaaacgca aggacatcgc cgtcggcaac
    12421 aatccgtact tcgtcggctt cggcgatgca gttggtttca tacttggcgg cgtagcaggc
    12481 tttggggctg gtggcgacat agtcgatttg cggcggcgcg ggcttgagcc cgttggtttc
    12541 gagacagagg aaatagcctt ccgccttgag cgtgtccagc agcatatcga gatgcggctg
    12601 gatggtcggc tctccgccgg tgatgatgat gttgcgggcg ggataagttt tcagacagcc
    12661 taagatgtcg gacaggctca tcataccaaa tgtcaaataa tcggtatcac accagccgca
    12721 cgccagattg catttgccca agcggacgaa aacggcaggc atacccgtgt ttcggccctc
    12781 accttgcagg ctttcaaaga tttcgacgat gcggtactgg ggattttcgg gggcgacgct
    12841 gatttttttc ataccgccaa caccgccgcc gccaaaaatg cgggcaaacc ttgtttgacg
    12901 agtatgcctt tgttgccgga cgaaaacgca ccccacgcgg cggcaatcag gacgaagccg
    12961 agaaacagta ccgtcgcgcc gtacacggca ttatccggag cggcaaactg cgcccacaaa
    13021 agcccgacgg caagaaagcc gttgtacagc ccttggttgc caaacagggt ctgcacgcgt
    13081 ttctgttcca taaattcgta aggcagcttg aacatttccg ccgctttttc gccgggaatc
    13141 tgcgtcattt caagccaggc gatgtagaaa tgttcggcgg cgacgaggag gacgagaatg
    13201 gtggagagga gtttcatggg tgttcctttc aatgggtgtg ccggtatgaa atgccgtctg
    13261 aaaaaacggg gaatccgtgc aggagaaaaa accgctttac ggtatagtgg attaacaaaa
    13321 atcaggacaa ggcggcgaag ccgcagacag tacagatagt acggaaccga ttcacttggt
    13381 gcttcagcac cttagaaaat cgttctcttt gatctgaggc gaggcaacgc cgtaccggtt
    13441 taaagttaat ccaccatacc ttatttgaat tgactatatc gatacgttca aaatattccc
    13501 tccccccctt catactccgc gcacgatgtc ggcgtttccc acaatttgac gcggcacacg
    13561 ttcagccccg cgtttttcag acggcagtac atttcgaccg ccatattttc ggcagttgtg
    13621 cggcagggca ggcgcagggt tttcatgttc cagccttcca aaagcgcggc gatttgagat
    13681 tcgcgcccgt tgccgccgtg gtagatgaag gcgtggtcga aatggtcggt aatgtgttgt
    13741 ttcacaatgg ctttcaagtc ggtaaagtcc atcaccatac cgtctttcgc tccgcctttg
    13801 atggtgccgt ctgaaacggt gatttcgagt ttgtaggtat gtccgtgcag gttttggcat
    13861 ttgccgtcat gcccgtcgag catatgcgag gagtcgaagg taaatatctt ggtaattttc
    13921 atcccgattc cttttcagtg aaaaccccgc cgctttccgc ccggagggtg gcttgatgtg
    13981 tcgttttgcg tggaaacata tccgctgctt caaatgggca aatagccttg aaagccgatt
    14041 tcggcgggtt tgccgtgcgg attcggggtc aggcggatat ggaaattgcc gatgtgctcg
    14101 agcgcaagca ggttgtccac gtctttcaag ttgtctttat gctgccgaac atagtcggtc
    14161 aacagggttt gcagggcttt ttgtttggct tcggcaggcg tggcggcggc gaacagcccg
    14221 aacgcgtgcg cctcggcaag ctgccccgcg cgatagccgc cgacattggc aaaatacagg
    14281 cgcggagcgt tttcaggcgg cattttcgtt ttgggcgttt cggatacggc gatgtcgtaa
    14341 ccgtccgccc attcgacaac ctgccaaccg tcgatgtgga ttttgtccgt atcgccgaac
    14401 cacgcggctt tgagtgcggg gacggcttcg cggtagtcgt cgcacacggc aaattggatg
    14461 tcgtgcactt cgatattcga ccggccggca ttgccgccga ggtaaaacat atggagcttg
    14521 ggcatatcgt attccttgtg ctttgcgcgc gttcagacgg cctttttact ttccagacat
    14581 tccgccagcc cgcgttcgcg caagatacag ctcgggcatt cgcggcagcc gccgacgata
    14641 cccttgtagc aggtgtgggt ctgttcgcgg atgtagtcca acacgcccat ttcgtccgcc
    14701 aacgcccacg tttgcgcctt ggtcaggtac atcagcggcg tgtggatttg aaaatcgtag
    14761 tccatcgcca gattaagggt aacgttcatg gatttgacga acacgccgcg gcagtcggga
    14821 tagcccgaaa aatcggtttc gcacacgccc gcgatgatgt gccggatacc ctgccctttg
    14881 gcaaaaatgg cggcgtaaag caggaaaagc gcgttacgcc cgtccacaaa ggtattggga
    14941 acgccgttgt cggcggtttc gatggcggcg gtttcgtcca tcagggcgtt gtgcgtaatc
    15001 tgccgcatca ggctcaaatc gagtacggtt tgactgacac ccaaatcctg cgcgatccac
    15061 tctgcgcgtt ccagctcgac ggcatggcgt tgcccgtatc ggaaggtgat ggcttggacg
    15121 ttttcgcgcc cgtaggtttg gattgcctga atcaggcagg tggtcgaatc ctgaccgccc
    15181 gagaagatga ccaaggcttt ttggtttgac atatcgaatc ctagttttgg taacgcggga
    15241 ggtttgcgaa ccgcgaagtt ggacagtaaa gacgcggatt atacaggcaa acgcgttcag
    15301 acggcatctg aacgccatgc cgtctgaaca cgcggcgtat gccgacttga cggcgagttc
    15361 cgccaagcgt ctgccttgtg cgaaggcgat attgttttct tcggcggtca ggacgggagc
    15421 cgctgtcggg aatatcttta tttgacggct tcgcagacgg cggacacttt ggggacggtg
    15481 cgcaatacgg cttcgcaacc ttcaacgctg tcgatgccgc gagtgattcg gcgtgcggga
    15541 ttgcgggtgc tgccgttttg ggaatagtag aggacgagga ttttgagggg atttgggttc
    15601 atgggcggat tccgttaaaa tagtttggtg ttcagacggc ataacggttg tccagatgcc
    15661 gtctgaaaca gtttgaaaac atgaaacctg gtaagagatt atgacctttt tacaacgttg
    15721 gcaaggtttg gcggacaata aaatctgtgc atttgcatgg ttcgtcatcc gccgtttcag
    15781 tgaagagcgc gtaccgcagg cagcggcgag catgacgttt acgacactgc tggcactcgt
    15841 ccccgtactg accgtaatgg tcgcggtcgc ttcgattttc cccgtgttcg accgctggtc
    15901 ggattcgttc gtctccttcg tcaaccaaac cattgtgccg cagggcgcgg atatggtgtt
    15961 cgactatatc gacgcattcc gcgatcaggc aaaccggctg accgccatcg gcagcgtgat
    16021 gctggtcgta acctcgctga tgctgattcg gacgatagac aatgcgttca accgcatctg
    16081 gcgggttaac acgcaacgcc cctggatgat gcagttcctc gtttattggg cgttgctgac
    16141 tttcgggcct ttgtctttgg gtgtgggcat ttcctttatg gtcgggtcgg ttcaagactc
    16201 cgtactctcc tccggagcgc aacaatgggc ggacgcgttg aagacggcgg caaggctggc
    16261 tttcatgacg cttttgctgt gggggctgta ccgcttcgtg cccaaccgct tcgtgcccgc
    16321 ccggcaggcg tttgtcggag ctttgattac ggcattctgc ctggagacgg cacgtttcct
    16381 gttcacctgg tatatgggca atttcgacgg ctaccgctcg atttacggcg cgtttgccgc
    16441 cgtgccgttt ttcctgctgt ggttaaacct gctgtggacg ctggtcttgg gcggggcggt
    16501 gctgacttcg tcgctgtctt attggcaggg cgaggccttc cgcaggggat tcgactcgcg
    16561 cggacggttt gacgacgtgt tgaaaatcct gctgcttctg gatgcggcgc aaaaagaagg
    16621 ccgaaccctg tccgttcagg agttcagacg gcatatcaat atgggttacg atgaattggg
    16681 cgagcttttg gaaaagctgg cgcggtacgg ctatatctat tccggcagac agggctgggt
    16741 tttgaaaacg ggggcggatt cgattgagtt gagcgaactc ttcaagctct tcgtgtaccg
    16801 cccgttgcct gtggaaaggg atcatgtgaa ccaagctgtc gatgcggtaa tgacgccgtg
    16861 tttgcagact ttgaacatga cgctggcgga gtttgacgct caggcgaaaa aacagcagca
    16921 gtcttgagta tttttgaaac tgtattttta tcctaaagac attttcctat ctgctgattc
    16981 caccgtttcc tttctgtgtg ccgccataat ttttataata gacaagccat tcaagtttgc
    17041 attgactgct ttttcattgg caatatccaa atgactggcc gcttcatcta aaaataggat
    17101 tttcggttcg caatataagg cccgcgccaa tacgatacgt tgtttttgtc cgcctgacag
    17161 tgcgctgccc atatcgccga tcaaggtttc atagcccata ggcatggcgg agatttcttc
    17221 gcgaatcata gcaaggcagg cgcaatgttc gatttttcct ctatccggtt ctgtatcgaa
    17281 aaaacagata ttctctgcaa ttgagccagc aaatgcaaat atagtggatt aaatttaaat
    17341 caggacaagg cggcgaagcc gcagacagta cagatagtac ggcaaggcga ggcaacgccg
    17401 taccggttta aatttaatcc actataaaac atcgtcctgc ctgaccatcg cactcaaatt
    17461 gcggataaaa cgtggcggta agctgtaaat gtcgtgccca tttatcatga ctttgcctga
    17521 ttcgggaggt agattgccgc ttaaaatatc caaaaatgtc gatttaccgc agcccgatcg
    17581 tccgattaac gcaagcgatt caccacgtcc gatcttgaag ctgacgtttt gtaaaacata
    17641 tggctcgtta tcggcataac ggaaagaaac attttccact gagatttcga tgtcgtctga
    17701 aatatcagga atgcctacat ccggcgtagc ttccgattct gtttcattca atacggtgtc
    17761 agctaaccgt tccgcatgaa gtgacagcat tttaatttgc acaaactgat cgaccagcga
    17821 gccaatacgt ccttcaaact gtcccttata agccaaaaaa gccattagta cgccgacggt
    17881 aaagacgccg tccaatacca tgcccgcagc taagtaaata atcaaaatat ttgccaaacc
    17941 gaaaagcagc ttgtttgcca agccgaacca aattcccagt ttctgcaccg tcagtcccgt
    18001 attaaccgta tccaccagca gccccatcca tgcagaatat cgttgaggac ctttaccaaa
    18061 ttgcttgact gctcggatac cgcgtaccgt ttccataaaa tagctgcttt gtttggcagc
    18121 atggatgatg ttttcctccg tcgcccgtcg caacggataa taggctaccc aacggactgc
    18181 gatataaact accagtgtcg ctgcaaccaa acctgtcagt atcgggctgt acactatcat
    18241 tagcacaaaa gtaaaaacag ccgttaggct gtttaaaacc aacacgaaaa acgttgaagt
    18301 cagagttccc tgaatactat ccaccgcccc gaagcgcgat accacatcgc ccaaatgccg
    18361 tttagcgaaa taatctgtag gcaaatctat caaacgtttg aaaacgtttg atttccactg
    18421 aacatttaac gtcgttgaaa aatgcatgcc caaccagccc tgcatgacag acaccgtatt
    18481 ttgtaccaga agtagcaagg taaagcccaa agccagcgtc gctaacagat tacgatcgcc
    18541 cgttaccact acatggtcga tgacccattg cataaaaaac ggcgacacca aagcaaacac
    18601 ttccattgct gctgccaaag ccaaaagctg aaacaatgtc cgccccagcc cagaaatccc
    18661 gcgcaacatg ggtaggatgc ggatttcctg cttttcttcc cctgcctcga aacgcgtgtt
    18721 tggccacagt tccaaagcaa ttcccgtaaa cttgcgcgat atttcctccg tcttgacttt
    18781 gcgtcgtccc gaagccggat ccatgacggc agccccgtcc gaagataccg attccagcac
    18841 cacaaaatga ttcaaatccc aatgtagaat acagggcagg cgcaaactgc ccaattcgtc
    18901 taaatccagc ctcaacgccc gtcccgtcag ccccatatca tcagcaaaac gaacaatatc
    18961 tgccaaattc tcacccttaa gtgacagaca gtattttgaa cgcagtgcgc gcaaatccgt
    19021 atagaaacca taaaatccgg ccacagccgc tagacatgcc aagccacact ccgctacttc
    19081 tgtttgcagt ataacgggca gcttttttgt caagcccaaa gacaggtttt gcagataatc
    19141 catagatttc cttgttgaat cagaaaagag agatgggaag acgggaatgg tttggaaagg
    19201 ccgtctgaaa aggtttcaga cggcctgcag ccccatattg actttcaatg cgaccggttt
    19261 tcctcgcgtt tcaaatacag cagatacgca agaaaagaca aagcccctac aggtcctgcg
    19321 acggtaaccg aaaaaaacct gcctttgtaa aacacaccgt cgaagtttaa aaaatagcaa
    19381 aggatgtcgg tcaggatgct gatggcaaga taaatcagca cagcggcttt ccagtgtttc
    19441 aggtaatgtg cgaaatccat ttcaaactcc cgatcaacag ttgcacgggt cgcaatggca
    19501 gtcttctccg cattggcagt tgcgcctgtc gatcattttt ccccattttg caccggcaaa
    19561 accgccgata tgcccaccca atgcagaacc gccctcagct cctacccatc cgccgcctgc
    19621 gccgccgatg gcaattccgg caggatttcc gcctgttacc aatgcgccgc ccgccattcc
    19681 cgtgaatgct ccggcaaacg accctaaagc gccgccgacc ttgcttccga cccattctcc
    19741 ggagactctg cccctgtgcc cccaagtacc ttctgtgccg ccgaaaacag cggtcatttc
    19801 gttcatggtc aattctctca tttcaaaact ccttttatcg tcaatcgaaa aattgaaagg
    19861 gcagataccc tatccttgct tttacagaca gagtatctgt aaaagggggt agtttcaggc
    19921 agcttcagtg tttatgtttt tatgtagtgt tttacaggtc gtctgaagag gtttcagacg
    19981 actttttatc tttcagcctc aagccttaca acctgcccga catactataa atcggctcca
    20041 atacccattc gtacagccgc cgtttctcgt gtaggatgtc tgcttccaac gtcatgccga
    20101 tttgcagcgg tttttcctca ccgtatgcag tgatggttgg tttgtcgggt tttattttca
    20161 cgagataaac aggttcgttg ctcttcgcca aatcggagga taccatgccc aatcccgaca
    20221 attcctgtct gcccagtgcc gtttttgcca ctgatacgac actgccggaa gcaagcccga
    20281 atttttgata gggatatgcc tgataacgca ggacaacctt gtctttcggc ttgataaagc
    20341 ctgctgcact gctggggata tatagatggg catataactc ggtacgttcg ggaacaatgc
    20401 tcaagaacag tttggaagga tcaacctgcc gtccgacttc gacgttcggt attgctatat
    20461 aacccgaccg tcctgcacgg atgatttgtt cagagcgcat ttcaaaatcc aaaacttctt
    20521 gagaaatatc ggcaatggtg cgttcaagct ggctttgttc tgtctcatgc cgtttgggga
    20581 ggctggccaa tgtcagattc tgcgtgcgga tttcctgaag cagcccggct tcttctcggc
    20641 ggtaggcatc aagtttggct ttctgctcta aaagctctgc ctcgacattc atcatttctt
    20701 gttttgacac tgcatcattg gcggatagga aacgatattt ccgcaacatt tcttccgcaa
    20761 gtctaatgcg ccttttctgg ccgtctatct gttgcgaaat atggagtttc tggttttcca
    20821 aacgttcgac agttgcttta aggctgcgcg tttcattctc gtgtatcagc ttcagacgac
    20881 ccagttcctg ttctgccaac gttttcttca aaactgcctc cgttttcaac tgctgctgca
    20941 cgctacctcc tgcgccgaaa cgtgaggtcg aaagcgcaaa tagcttgtcg ccagccttaa
    21001 ccttttctcc atcttccacg aatttcgctg taattgtccc cgtatccggt gcatacaccc
    21061 tgattacgcc cgatgcaggt agaatttgtc cctccattgt tgtctttcgc gtatagttac
    21121 cgaatatcaa aaacaggata atcagtaacg cagatatcga tgcaaatgtc gtccacaggg
    21181 aaaatgacaa cgggcgtgtc agaatcactt tacccgtcag gctggtttgg cgggcaatgg
    21241 cgacttcagg gcggaagaaa ggttgcttgg gtctattcat aaaattgaag ttaagaaagt
    21301 ttcagacgac ccctagagat cgtctgaaca atgagaaata tcagcagtaa tctgtaccgt
    21361 cagtgtagcc gtttcctgat ttatctgctt ttgttgcggg agcagttaat ccatgttcaa
    21421 tctcaaagat tggtcttccg ttataaggag gtgcattaac ggcatcattt acccaatcac
    21481 gagtcagatt gtatacacca tttgcaccat atagcaccga aaaaattttt cggcaggaaa
    21541 tgaactgtcg ctgcagcagc aggtattgca acaaaatccc cccatgtggg acctcctttg
    21601 gttgtggcag cattagctac atttccagct atattgtctg ttacaggacc tccccctgaa
    21661 accagcttca attcatgaag ttgaagttca atcattttta tactcctttt cttggttggt
    21721 attcctaaaa attcggctaa caaaaacata tggcagatat attgaaaaaa aattcaaagt
    21781 accctgaata aaattcaaat tccaactata tttgttaatg tagtcgagaa gaaacatatc
    21841 tgataaagaa tatagcaatt gataacaagc tattactaat attacgaaaa atataaactg
    21901 cttccagttt ttcatagaat ccctcacaaa atttccagaa aatctaactc tatcaactga
    21961 tagatcaact tcctaacttc ttcatgtttt gcctgattga agttaaccag tagatttttc
    22021 aacaataacg gttcattctt accgatgtgt tctaacactt ttttccccaa ctcatctacg
    22081 cttatcttca tcccattccc aatcaaatat cccttttcca acgtatccaa attattggca
    22141 tttagtctca acctgacgtc gtctgaaagc ggagtagcgt tgggattcgc gaactgttcg
    22201 agatgaaaag cggtatcggt acgttctttg ccgagaaagt cttcactgaa agcttcgtaa
    22261 ttgacggggt cggcaatcat ggcggcaatt tgtgcggcag tatcgttgat acgcgtccta
    22321 tcttgctccc agtctgagaa actgtggcgc agactttcta tggtgggaaa tttcttcatt
    22381 agccactcga ggtaattata gccgtttggt gggaaggtac cgacagcgaa gtggaaggtt
    22441 tcacagccga gcgggatagg tctgtgccac caaccgcgtg ggatgtagag gacatcacct
    22501 gcttcaagga taatatccat atcgatatgt tcaggaatgg aaatatcagt atctttagtc
    22561 tgttgcatat acaatggcat agggaaatca ggggcagtaa gttgccaacg tttcttgccg
    22621 aaaagctgga cggcatacat atctcgggcg tcccaatggt ttttataaga ttcgtcgctg
    22681 ccaaaagcaa gatatccact aacaatagta tgtgcgccgg caaagcgggc gacttgacgg
    22741 gcgatatggt ctgaaaacgg ctcgttgtta atatggttat agactaacga cgcaccgttc
    22801 ttcatatgtt cgtagataac ggatttaata aaacggtagc gagttttgcc caaatcgtcg
    22861 aaactttcga cgtattcttc tttaggaacg attgcgcctt ttttacgcag atgaaacagc
    22921 ggtgcggttg ggtctgctcg ttggtatatc tcgttgatat atttacaaga tgcggcttcg
    22981 agattccgaa ccgctccttt aaagagcttg ggcttttgat acagataagt ctgtcggaac
    23041 gttttaggac taatgccgaa gtcgagatgg atgcccatta cttcccctta ctcagaaaat
    23101 atttaaaatt tataatgtta catatagtta caaatattag agttttttgt gtgtgcgtca
    23161 aggaattgtt gacaatttta gttaaaaatt tgtctccaaa cggaaaaaag cggtttgttt
    23221 tttgttgtta acatttttat tgtaattaaa taaaaggtcg tctgaaaacg gttttcagac
    23281 gaccttttgc tataatcggg cttcatcgcc ccgttcggtt tggaacctta tgaaaaccct
    23341 cgtcctcctc ctgctttcct tctccacgac caccgctttc gccgcatacg gtttgggttt
    23401 ggggcaggca ccgaaatatc ctgccggctt tcgcgcctac ggttatgttt attccggacg
    23461 gcagggctgg gttttaaaaa cagaggcgga tgccattaaa ttagacacgc ttttcaaacg
    23521 ctttgtgtac cgtccttccg ccgccaatca aaaccccgtc ggacagcgtt cggacggcat
    23581 acccgccaac cacacaaagg aaaaaccatg agcaaaaaaa tcaaagccgg cattgtcggc
    23641 gcgacgggct acacgggcgt ggaactgctg cgcctgcttg ccgcccatcc agatgtcgaa
    23701 gtcgccgccg taaccagccg cagcgaagcg ggaaccgcag ttgccgatta ctttccaagt
    23761 ctgcgcggcg tgtacggcct cgccttccaa acgcccgacg aggcaggttt ggaacaatgc
    23821 gacatcgtct tcttcgccac gcccaacggc atcgccatga aagacgcgcc gcgcctgatt
    23881 gaacagggcg tgcgcgtcat cgacctttcc gccgacttcc gcatacggga cattccgacc
    23941 tgggaacact ggtacggcat gacccacgcc gcccccggcc tcgtttccca agccgtgtac
    24001 ggattgagcg aactcaaccg cgaagccgtc gcacaggcgc gcctcgtcgc caaccccggc
    24061 tgctacccga cctgcgtatc cctgccgctc gtgccgctgt tgcggcaatg ccgtctgaag
    24121 cccggtatgc cgctgattgc cgactgcaaa tccggcgtgt ccggcgcggg caggaaaggc
    24181 aatgtcggtt cgctgttgtg cgaagccggc gacaacttca aagcctacgg cacagccgga
    24241 caccgccacc tgcccgaaat caggcagacc atcgccgggc ttcaggacgg catcgccgaa
    24301 ggattcgtgt tcacgccgca cctcgcgccg atgatacgcg gtatgcacgc caccgtttac
    24361 ctccaccttt cagacggcag cgaccccgaa accgtcctgc gcgactacta ccgcgacagc
    24421 ccgttcatgg acatcctgcc cgccggttcg acacccgaaa cccgcagcgt gcgcggcgca
    24481 aacctctgcc gcatcagcat ccgtcaggcg gcgcaatccg atgtgtgggt cgtcctttcc
    24541 gtcatcgaca acctcgtcaa aggcgcggcg ggacaggcgg tgcaaaatat gaacattatg
    24601 ttcggactgg aggaaacaca cggcttgggc ggcatccccc tgctcccctg aagcgcaaac
    24661 ggcaaaccgc aggcatcgtg cctgcggttt tttgatgccg tctgaaagcg gcggtttttt
    24721 tgggttcgga cggcttttgg ctcatcattc cgcgaaaaca aaaatctaaa ataccgtcat
    24781 tcccgcaaaa gcgggaatct agtttatccg gcttcagcaa tttccgacac atttccacac
    24841 gcttcgattc cgtcatttct ccggtttcaa tcgtttccga taacaccgtg gtttttcatt
    24901 tctagattcc cgcctgcgcg ggaatgacgg cggggggctt gccgttttcc cgataagcct
    24961 ctgcggcttc tcattgccgg atttccgccc gcttgggaat aacgggattt aaaatcatga
    25021 aaatgtgtca aaaataatat agtggattaa caaaaaccgg tacggcgttg ccccgcctta
    25081 gctcaaagag aacgattctc taaggtgctg aagcaccaag tgaatcggtt ccgtactatt
    25141 tgtactgcct gcggcttcgc cgccttgtcc tgatttttgt taatccacta taaaaaatca
    25201 gatttccgtt acactttttt ctaatatttc agacggcatt ttgctcacac acccaaataa
    25261 ccttccctgc cggaaagcca ccttgccaaa tgcgcttcga cgatttcagg gttttgttca
    25321 atcagcatcg gggcggtttc gcgcgcttgt tccaaaaggt gtaagtcttc ttcgagcttg
    25381 gcgaagcgca gcataggcac gccgctttgg cgcgcgccga gaaattcgcc ggggccgcgg
    25441 atattgaggt cttggcgggc gatttcgaag ccgtcggtgt gttcgtagat gactttcagc
    25501 cgcgctttgg cgagttcgcc caagggttcg gcaaacagga ggacgcacac gctttccccc
    25561 gcgccgcgcc ctacccgtcc gcgtaattgg tgaagctgcg ccaagcccat gcgctcggcg
    25621 tgttcgatga ccatcagggc ggcattgggc acatctacgc cgacttcgat aacggtggtg
    25681 gcgaccaaga cgttcagacg gcctgcggca aattccgcca tgacttcggc tttttcggcg
    25741 gccttcatgc gcccgtgtac cagtccgata ttgggttcgg gcaatgccgt ctgaagccgg
    25801 gcgagggttt cggcggcggt ttgcagttgc agggtttcgc tttcttcaat caatgggcag
    25861 acccaatatg cctgccgccc ttttcggcaa gtgccgagga cgaagccttc gacttcggcg
    25921 cggcggacgt tgttgacgag gcgcgtttta atcggagtgc gcccgggcgg caattcgtcg
    25981 atgacggata cgtccaagtc agcgaagaaa ctcatggcga gcgtgcgcgg gatgggtgtg
    26041 gcggacatca tcagctgatg gacttcgcgc cctttgtttt tgagtgcgag gcgttgtgca
    26101 acgccgaaac ggtgctgttc gtccacaatg ctcaagccca aattgtgaaa cgccacgccg
    26161 tctgaaaaca gggcgtgcgt gccgacggcg attttgacgc tgccgtcggc gagtttggct
    26221 ttggcttcgt ctttggcttt tttgcgcaaa ctgccaaaaa ggcagacaac ttcaatgccc
    26281 aaaggttcga gccattgttt gaacttgatg aagtgctgtt cggcaaggat ttcagtgggc
    26341 gccattacag ccacttgcgc gcctgattcg atggcggtca gcgcggataa ggcggcgacg
    26401 atggttttgc cgctgccgac atcgccttgc agcaggcggt gcatggggtg ggtttgcgcc
    26461 atatcgcggc agatttcgga aacaactttt tcttgtgcat cggtcagggc aaacggcagg
    26521 gcttggcgca gggcttgggt caatgtgccg tcgccgcgca atgccgccgc cgtgccgctg
    26581 acgcgcttct gccgcgccaa acgcatggac aactgttgcg ccaaaagttc gtcgaatttg
    26641 agccgctgcc acgccggcag cgtgccgtct gaaagttgat gaatagtgaa actcggcggc
    26701 ggcgaatgca aaaggcgcag gctttcggca aggcgcggca gcttcagacg gcctaataaa
    26761 gcatcaggca aggtgtcgtg cagcggcgta acgtccaacg ccgtctgaat gatgcggcgc
    26821 aaagtgggct ggttcaaacc gtttacggtc gggtaaaccg gcgtgaggct ttccgccaaa
    26881 ccgccgccct cggcatcgcg gattttggga tgaatcatct cgtcgccgta aaatccgtgt
    26941 ttgatttcgc ccacggcgcg gatgcgtttg ccgaccgccg tctgcttctg atggctggcg
    27001 taaaagtgga tgaagcgcag aaaaaggacg ctgccggaac cgtcggcaat ttgaacaatc
    27061 agctgcttgc gcggtttgaa cgttacttcc tgatggataa cctcgccctc gacctgacac
    27121 ggcacgccaa tcggcgcgtc cttaatcggc ataatgtgcg tctcgtcctc gtaacgcagc
    27181 ggcaggtgca acaccaaatc ccacgcggta tggaggttga gtttgtcgag cttcttggcg
    27241 gaaacatcgg tgattttgag ctgtttctgg gtttcgggcg acatcatagg cagattcctt
    27301 tggacgcgcc tattttatcc gaaaacaaaa atgccgtctg aaacggattc agacggcatc
    27361 ggcaggcagg aatcaagccc cggcggcttc ggcttcctgc tgttgttggt aaatcgcttc
    27421 aaaattaatc ggcgcaagca ggacgggcgg gaagccggcg cgcgttaccg taccggaaac
    27481 agcttcgcgc gcgtaaggga agaggatgtt cggacacgcc acgcccaaca gcagctgcac
    27541 atcttcttcg gggatgtttt ccagacggaa aataccgctt tgggttactt cgttcaaaaa
    27601 catcgtgcgc tcgttatcca atttggcggt aacggttacg gtaacgtcca cgtcgtagta
    27661 gccgtcttcc agcttttggc tgccggtgga aacgcgcata tccacttcgg gatcgccctg
    27721 ttccaaaaag atttgcggcg cgtgcggcac ttccaaagac aagtctttga catacagtcg
    27781 ctcgatgctg aatacgggtt gcagttcttc gctcattttg ttttcctagt tgtgggttaa
    27841 gggttcagca gtccgtccag cccgccttcc tgctgaaggc ggtagaggtc ggtaaatcct
    27901 ccgacgtgcg tttcgccgat gaaaatctgc ggcacgctgc gctgtcccga aagccgctgc
    27961 atttcggcaa aggcttcggg gcttgcatcg acacggattt cgtcgatatg tccgacacct
    28021 gccgcgtgca gcagcctttt cgccatcgcg cagtaggggc aaaacggacc tgtgtacatg
    28081 gtaacggtct gcatattggg tttccgaaag ttttgcaatg ataatcaata taggggcaat
    28141 cgctccgttt ggcaagtgcg gaacagatgc acgttcagac ggcatgtgcg gagtgtgtca
    28201 aagtttcttt tttaaagtat gataggcatt gtgaaaaata tttttgcacc cgctctgcgc
    28261 ggcggggcgg aacggatgca aaaatatttt tattacattt tcaggaaaaa ccatgttgtc
    28321 aggactcccc atccccaaag acatcgcgcg cccgcccgaa acgatattgg tcaacatcac
    28381 gccgcaggaa acgcgcgtgg cggtgttgga ggaaaacaat atctgcgagc tgcacatcga
    28441 gcgcaacagc gggcacagcc tggtcggcaa tatctatctg ggcgtggtgc gccgcgtgct
    28501 gcccgggatg cagagcgcgt ttatcgacat cggcttggaa cgcgcggcgt ttttacacat
    28561 cgtcgatgtc ctcgaacaac gccgcaaccc cgaagaaacc cagcgcatcg aacatatgct
    28621 gtttgaaggg cagtcggttt tggtgcaggt catcaaagac ccgatcaaca ccaaaggcgc
    28681 gcggctttcc acccaaatct cgctggcggg gcgtttcctc gtccaccttc cgcaagaaga
    28741 ccacatcggc gtgtcccaac gcatcgaaga cgatgccgaa cgcagcagcc tgcgcgaacg
    28801 cctcgacaag ctcctgccgg aaaatgcctg ccggggctac atcatccgca ccaacgccga
    28861 aaacgccacc gacgaacagc tccagtccga catcgactac ctgaccaaag tgtgggaaca
    28921 catccaagaa caggcgaaaa tccggccgcc ggaaaccctg ctttatcagg atttgccttt
    28981 aagcctgcgc gtgttgcgcg atatggtcgg ctgcgacacg cagaaaatcc tcgtcgattc
    29041 caccgtaaac cacgggcgca tgacgcgttt tgccgaacaa tacgtccacg gcgcattggg
    29101 caggatagaa ttgttcaaag gcgaacgccc gctgtttgaa acccacaaca tcgaacagga
    29161 aatcagccgc gccctgcaac cgcgcgtcaa cctcaacttc ggcagctacc tgattatcga
    29221 gtccaccgaa gccatgacca ccatcgatgt caacaccggc ggcttcgtcg gcgcgcgcaa
    29281 tttcgacgaa accatcttcc gcaccaacct cgaagcctgc cacaccatcg cccgcgaatt
    29341 aaggctgcgc aaccttggcg gcatcatcat catcgacttc atcgatatgg cgcaggaaag
    29401 ccaccgcgaa gccgtgttgc aggagcttgc caaagccctc gccttcgacc gtacccgcgt
    29461 taccctgcac ggttttacca gcctggggct ggtcgagctg acgcgcaaac gctcgcgcga
    29521 aaacttaaac caagtcctct gcgaaccctg cccttcctgc caaggcaggg gccgcctgaa
    29581 aacgccgcaa accgtatgct acgaaatcca gcgcgaaatc gtccgcgaag cgcgccgtta
    29641 cgatgccgaa agtttccgca tcctcgccgc ccccaacgtc atcgatttgt ttttggacga
    29701 agaatcgcaa tccttggcaa tgctgataga tttcatcggc aaaccgattt ctttggcggt
    29761 cgaaaccgct tacacgcagg aacaatacga tattgtgctg atgtaaaaaa tgccgtctga
    29821 agccttcaga cggcatctgt ctatttcagg gtttccttgt ccaacagcgc gcgtatcaac
    29881 agcccgtgtc cgaaacgtcg gctgtcggtc agttccaaat atccgccgta ttttttggcc
    29941 agcgtgtcgg caatcgacag ccccagcccc gccccctgct gctccgttcc caaaatacgg
    30001 taaaacggat cgaggacgcg ggcgcattcg gattcgggaa tgccgttccc gttatcctcc
    30061 acccacacgg cgagatattt cccttcgtcc gtgaaaccca aatctatcct gcccccgttc
    30121 ggcgtgtaac gtaccgcgtt gtcggcaaag gttttaacca gcgtatagat ttccgtttcg
    30181 tcggcagaca cttcgacatc gcctccgacc gccacaccga tgtcctgacg tttttccaaa
    30241 accagcggca tcagttcctg caacacttgg cggaaacggc tttgcagacc gaatgtcgtt
    30301 ttcgtcaaag gggtttcgtc cgactgcgaa cgcgccagtg ccaaaagctg ttcgagcagg
    30361 tgtttgttgc gcctgatgct ctgctgcaaa acggcggact gccgccccgc atcgggtggg
    30421 agcggcatat tgttgagccg ttccgcctga agggaaaggg cagtcatcgg cgtacgcaat
    30481 tcgtgtgccg cgtcggcgac aaaccgctga cggcggcgta tatcttcatc aacacgtttc
    30541 aaaagcagat tgatggcggt tacgaaccct ctgatttcac tgggaatatt gtacacactc
    30601 aaagcaggca ggccgttgat tcggcgttgt tcgagacttt gcgacaattt gcggacgggg
    30661 cgcatggctt tgtgcgtaat ccacacagtc agcagtatca tcagcggcag tgccgccaac
    30721 aggggcaaca cgctttgccg tgccgcatcc tccgccaaat cttcacggta ttcgttttcc
    30781 tgcatgacgg caatccgtcc ctgttcggtc gtgcggatat aggcgcggta ataatcgtcg
    30841 tccccgtccg cccgaagcgt gtgcagaccg tccgccagat gcgcgggcag gctgacaaca
    30901 gggtcttcct gctgcggcat ctgtaccaaa atacgcgtat cgccgtcgcc ttcgggcaaa
    30961 gtttcggatt tggaatcagg gccgacatac aatgccgcct gacggagcag gtcgtcctgc
    31021 aacgcttccg tttcgtggaa ggtttcgtag taggaaaaca tacctgcaag cattgccagc
    31081 ggaacaaaca tccaaatcag ggcaaggctg attctgacct gaagcgaatg ttttaaaatt
    31141 ttaaaaaaac ggtccggcat cttcgttcct gttcagacgg catcttgacg cggcatcagc
    31201 cagccgacac cgcgcacatt ttgtatgctt tccttaccca atttcttgca cagcccgtga
    31261 atcagaaaat ccaccgcatt gctttcgact tccccgcccc aaccgtaaac cttgtcctcc
    31321 gaatccgagc gcgacaaaat cacacccggc ctcgccagca aagcctgcaa taccgaaaac
    31381 tcctggttgc tcaatgccac ctgcctcccc tcggcaataa tttctacctg atacgttgca
    31441 ggattgaggg ataacgcacc atttgacaga cacgcctgcg cctgtccgct cccgcgccgc
    31501 aacaccgccc gcatccgcgc cttaaactcc gccatatcga acggcttgac gatatagtca
    31561 tccgcaccgc cgtccaaacc gttcagccgg ctgtacaaat catcccgcgc cgtaacgatc
    31621 agcacgggga cggtacagcc tgccgcgcgt atttccgaca aaacatccaa accgtcccgc
    31681 ccaggcaaac ccaaatccag cagcatcaag tcataaggct gggcagcggc agccgccgca
    31741 acctgcgcgc cgtttttgac ccaatccacc gcatagccgc cgtctttcaa acttgccgac
    31801 accgcttacg caatcatcgc attgtcttcc accagcaaaa cacgcatcaa ctttcccttc
    31861 aaaataaacc gtgcctattc taacacccca aaattagccg caatttagcg gcctttacgc
    31921 ttgccggtat ttttcaaaac tgcggcacaa aaaaaccgcg ccggcaactg ccttcagacg
    31981 gcattggggc gcgattgcaa cacacggaca ggcaggcaaa gcctgcgaca gatcacagga
    32041 acgattcggg cttcaggcgg cttcgccgtt tacggcagag gcacgattcc tgccgctatc
    32101 gaactgatca atatcgccag cgacaaaccc cacgcccaga aaaacgaata acggatgtgt
    32161 ttgcccatcg acaatttcgc caaacccaaa cccatccaca aagccggcga aagcggcgta
    32221 acaaaagtac cgacgatact gccgatcaac atcgcatagc ctgccgcttc gggtgcaacg
    32281 cccgcctgcg aggtaatctg ttccacaatc ggaaacagtc cgaaataata agcgtccgta
    32341 ctcaaaacca actcaagcgg aatacccaac acaccgatgg caatatgcag ataaggcagc
    32401 aacgcgtccg gcaggatatg cacaaggtct ttggaaatcg cgtccaacat ccccgcgccc
    32461 ttcaaaatcc ccaaaaacgt acctgccgcc aaaataatgg acgccatcat caccgcgccg
    32521 ccggcgtggg cataaatccg ctccatctgt tcctgcgggc tgcggtaatt caaaagcaac
    32581 gccgccgttg cagccagcat aaatacataa cccggcggga agatgcccga aaaaagcagg
    32641 ctcatcgccg ccaaaaacag caggacattc caccaaaaca gtttcggacg cgccaatttt
    32701 tgttcttctt ccgacaaagg cgccggcttt atcaaatccg ccacggcggg caacgcgccc
    32761 aactcccgga caatccgcct tttttcacgc acacccaaaa acagggacag cacaaggata
    32821 aacaccacac cgataatttg caccgtcaac aaaggtttat acaattcgcc cacatctgcg
    32881 cccaacacgc ttgcaacccg cccgatcggc ccgccccgcg gcaaaaggtt gattagcccc
    32941 gcgctggaag tcagcagcaa aaacagcagg taaggattca tatgcagacg cttgtaaagc
    33001 ggcaaaaggg cggggacgac cgataaaaac gtcgtcgcgc ccgccccgtc caactgtgcc
    33061 accaccgaca ccaagaccgt ccccacactc actgccacga tattaccccg agtcagctta
    33121 atcaaaccgc ctatcatcgg acggaacagc cccacatcgt tcatgattcc aaaaaacaaa
    33181 atggaaaaca taaacataat cacaatctgc gtcaccgatt tcgtgccgcc cgaataaaat
    33241 tcttttaatt gggatacatc aaaccccgcc agcaacgccc caatcagcgg caccaagatt
    33301 aatgcgatga tgggcgacac tttttccgtc agcaacagcc atacgatgac cccgataatc
    33361 agcaatccga taaacgtcag cattatttct cctttatttt attttgaaca taaaaccgcc
    33421 cgtgcaggca aaaccgccaa catatgcggg ataagccttg cattccattc acgcctgccg
    33481 acatttctgc atccgtccgt catttcacgg cggcaaccga tacggaacaa ccggcaaacc
    33541 ggtatcggga cggcgcgggg gcatccgtcc cggtgcaccg attcaaacga aactgccccc
    33601 tatcattgcg gagcgcgggg cgtgccgtac acgcgggatt ttaaacacgc cgatcatttg
    33661 ccgcgcatta tccggcaaac ggcaaacctt gacgctgccc ggcccatata aaaaagccgc
    33721 aaaacccgaa ccggttttgc ggcttttcga tttggtttgt ttatttggca tttctttcaa
    33781 tcaaaccgac aaactgattg atatatgact gaacaaaatc ccttgccgag tcaatcaact
    33841 taccgttttc atcaaacagc gtcggcgaat tgcccaaaaa tacttccggc tgtccggtta
    33901 cgggcatatc aaaatacgac agggcaaggc gcaggttttt ttgggaactg taaccgccca
    33961 tcttgccgac ggaatggctg atgatgccgg ccggtttgtt tttccaagcc acgtcggcat
    34021 tcggtttcga gccgatgtcc accgcatttt tcaaacaggc gggaatggtg cggttgtttt
    34081 cggacgtaac gaacaaaatg ccgtccgaag ccttaatcgt ttcgcggaaa gccgtgtagc
    34141 tttcgggcag cggcacatct tccaccgcag ggtcgtcata atcgaaattg taaagcggca
    34201 gatgtccgat ttcgacgatt tccgcctgcc agccttcggg gaacatctcc gccgcattca
    34261 atgccacttt gcgcgcaaac gaagcacggc gcaggctgcc caccaaaata ctgattttct
    34321 tagccacaat cattcctcct gaatattaag tttgtgcgtt tcaatcattt cataatggta
    34381 acgattatta tatatgcgat ttcccctgca aacaagccgg tccgccgccc cggcgttccc
    34441 acttatccgg ctttgcctta taattgcttt ttttatgtaa cagattcacc tatgaatttc
    34501 cccaaaacag cggcctccct gctgctgctt ctcgcctccc tcgccgcaca cgcgctcgat
    34561 accggccgca ttccgcaaaa cgaaatcgct gtatatgtcc aagagcttga cagcggaaaa
    34621 gtcatcattg accaccgtgc cggcataccc gtcaatcccg cgtccacgat gaagctcgtt
    34681 accgcgtttg ccgccttcaa aaccttcggc agcaattacc gctgggcgac cgagtttaaa
    34741 agcaacggta cggtaaacga cggcacgctt gacggaaacc tgtattgggc gggcagcggc
    34801 gaccccgttt tcaatcagga aaacctgctt gccgtccaac gccagttgcg cgacaaaggc
    34861 atccgcaata tcacggggcg cctgatgctc gaccacagcc tgtggggcga agtcggcagt
    34921 cccgaccatt ttgaagccga cagcggttcg ccgtttatga cgcccccaaa tccgactatg
    34981 ctgtctgccg gtatggttat ggtgcgcgcc gaacgcaatg ccgccggcag taccgacatc
    35041 ctcaccgatc cgcctttgcc gcatattttt gcccaaaaca acttgaaaat taccgcctcc
    35101 caagctgcct gcccttcggt caaaaaactg atgcgcgcat ctttttcggg caatacgctg
    35161 aaattgcgcg gcaatattcc cgaaagctgt ttgggcaagc ctgtcggtgt ccggatgttc
    35221 gcgcttgacg aactgatccg ccaaagtttt accaaccgct ggctgctcgg cggcggacgg
    35281 atttcagacg gcatcggcat agccgacaca ccggaaggcg cgcagacgct tgccgttgca
    35341 cactccaaac cgatgaagga aattttgacg gacatgaaca agcgttcgga caatctgatt
    35401 gcgcgttccg tcttcctcaa actcggcggc gacggcaaac tgcccgccgt ttccgaacag
    35461 gcggcgtctg ccgtccggcg agaacttgcc gtatcgggca tcgatgttgc ggatttggtt
    35521 ttggaaaacg gttcgggtct gtccagaaaa gaaagggtaa cggcgagaat gatggcgcaa
    35581 atgttggaaa cggcttattt cagcccgttt gcacaagatt tcatcgacac gctgcccatc
    35641 gccggcacag acgggacttt acgcaaccgc ttcaaacaaa gcggcgggct gttgcgctta
    35701 aaaaccggca cgctcaacaa tgtccgcgcc cttgcaggtt attggctggg cgacaaaccg
    35761 atggcggtgg tcgtcatcat caacagcggc cgcgccgttt ccctgctgcc cgacttggac
    35821 aacttcgttg ccaaaaacat catctccggc ggcgacggct ggctggatgc gaaactgatg
    35881 tgcaaagaac gccgcgcctg aatcgggaaa atataaaaat gccgtctgaa ctgttcggac
    35941 ggcatttttg attttcaaac cggaattaca gccccgctgc cgctttcaat gaggccgctt
    36001 tgtcggtgcg ctcccaagta aactcaggtt cttcgcggcc gaaatgtccg taggcggcag
    36061 attttccgta aatcgggcgc aagaggtcga gcatttggac gatgcctttg ggacgcaggt
    36121 cgaaatgttc gcaaaccagc gcaatcagtt tttcctcgct gattttaccc gtaccgaaag
    36181 tatcgatgga aatcgaagtc ggttcggcaa cgccgatggc gtaggaaact tggatttggc
    36241 attgggttgc caagcctgcg gcgacgatgt ttttcgccac ataacggcag gcgtaagcgg
    36301 cggaacggtc cactttggac gggtctttgc cggagaatgc gccgccgccg tgcggagccg
    36361 cgccgccgta ggtatcgaca atgattttgc ggccggtcaa accgcagtcg ccttgcgggc
    36421 cgccgatgac gaagcggccg gtcgggttga tcaggtattt tgtttcgtcg gtcagcagtt
    36481 cgggcggcaa aacgggcttg ataatctgct caatcacggc tttactcagt tcttcatggc
    36541 tgatggcagg atcgtgctgg gtggacagga cgacggtatc aatgcgtttc accttgccgg
    36601 tttcgctgtc gtaaaccacg gtcagttggg cttttgcgtc agggcgcagc caaggcaggc
    36661 ggccgtcttt gcgcaattcg ctttgacgct gcatcaggcg gtggctgtaa tagatggcaa
    36721 acggcatcag ggtcggggtt tcgtcgcagg cgtagccgaa catcaagcct tggtcgcccg
    36781 cgccttggtt caggtcgatg ccttcgcctt cgttcacgcc ttgggcgatg tcgggggatt
    36841 gttggtcgta gtacacgccg accgcgcagc cgttggcgtc aaagcccagc tcggaggagt
    36901 tgtagccgat gcgtttgatg gtttcgcgtg cgactttgat gtagtccact tgggcggtgg
    36961 tggtgatttc gcccgccaat acgcacaagc ctgtgttgac caaagtttcc gccgcgacac
    37021 gcgctttggg gtcttgcgcc aagatggcat ccaaaatcgc atcggatact tggtcggcta
    37081 ctttgtccgg atggccttcg gaaaccgatt cggaagtaaa cagatattcg ctcattgaaa
    37141 ttttccttga aaaacaaacc atcttcttca gacagcatgt tgtatgaaca taatgtcgac
    37201 agcgggaaat atagcaaaat ttccctattc gtgccattca gttgagaaat attcctattt
    37261 aaacggcgct ttggaatctc tgtccgtacg tttcgtacag gcaaaaaaat tcccgcatca
    37321 agcgggtttg gattgctccg gcgagccaca tcggtttttc aaccgtccac cttacctttc
    37381 cgtttgaaaa gcaggttggc atggaattcc caactcttaa tgcagcgcgc atcgtagcag
    37441 aaaaggcata ttgccgcaat acttcccttt ttcagacggc atgtttcgtt tacaattcag
    37501 gctgtttccc cctttgcgaa ccgccatgca catcctgttg accgccctgc tcaaatgcct
    37561 ctccctgctg tcgctttcct gtctgcacac gctgggaaac cggctcggac atctggcgtt
    37621 ttacctttta aaggaagacc gcgcgcgcat cgtcgccaat atgcggcagg cgggtttgaa
    37681 ccccgacacg cagacggtca aagccgtttt tgcggaaacg gcaaaatgcg gtttggaact
    37741 tgcccccgcg tttttcaaaa aaccggaaga catcgaaaca atgttcaaag cggtacacgg
    37801 ctgggaacac gtgcagcagg ctttggacaa gggcgaaggg ctgctgttca tcacgccgca
    37861 catcggcagc tacgatttgg gcggacgcta catcagccag cagcttccgt tccacctgac
    37921 cgccatgtac aagccgccga aaatcaaagc gatagacaaa atcatgcagg cgggcagggt
    37981 gcgcggcaaa ggcaaaaccg cgcccaccgg catacaaggg gtcaaacaaa tcatcaaggc
    38041 cctgcgcgcg ggcgaggcaa ccatcatcct gcccgaccac gtcccttctc cgcaggaagg
    38101 cggcggcgtg tgggcggatt ttttcggcaa acctgcatac accatgacac tggcggcaaa
    38161 attggcacac gtcaaaggcg tgaaaaccct gtttttctgc tgcgaacgcc tgcccgacgg
    38221 acaaggcttc gtgttgcaca tccgccccgt ccaaggggaa ttgaacggca acaaagccca
    38281 cgatgccgcc gtgttcaacc gcaataccga atattggata cgccgttttc cgacgcagta
    38341 tctgtttatg tacaaccgct ataaaacgcc gtaacgaaaa taaaaatgcc gtctgaacag
    38401 gtttcagacg gcatcttgtc atttgacgat ttcggacaac gaccaacgcg gacggacgtt
    38461 gaacgcaccg acctccctgc ccttgcccag gtgcatcgca ccggcaaagg caatcatggc
    38521 accgttgtcc gtgcagtatg ccatcggcgg gaaaaacacg ctgacttttt cggacggatg
    38581 tttcggcttg cctttggggg tcgggatttg taccgtcatg ttgccgaaag tttcacggag
    38641 tttgcggttt gcaccgaccc cgccggcgac cactacggtt ctgaaccctg tctgcaacag
    38701 ggcttttttc acttttgcct ccaacacttc tactaccgca tcttgaaacg cacggcaaat
    38761 attgttgcgg gtttgctcgg gtatggtttc cgaaccgttt gcctcacgca ctttttcaac
    38821 ggcagtcaaa acagcggttt tcaatcccga aaaactcatc tgcaaatcgt cggaatgaat
    38881 catcgggcgc ggaaaaacaa acgcttcggg cctgcccgat tccgcaagtt ccgacagttt
    38941 cgcaccgccc ggatacggca aacccagcag tttcgccgtt ttgtcgaatg cctcgcccgc
    39001 cgcatcatcg acgctctcgc ccaaaagctc gtaatcgcct atgcccctga ccgccataat
    39061 ctgcgtatgc ccgcccgaaa ccaacagcgc gacaaaagga aagtcgggtt tttcctccgc
    39121 caacagcggc gacagcagat gtccttccaa atgatggacg gggatgacgg gcttatccaa
    39181 cgctaaagcc agcgcgttgg cgtagctcga acccgccagc agcgcaccgc ccaaaccggg
    39241 cccctgcgta aaggcaaccg cgtcaatatc gccatacgat gcgcctgcct gcgccaaaca
    39301 gccttcagtc aacggaacaa ggcggcggat atggtcgcgg ctcgccaatt ccggcacgac
    39361 cccgccgtat tccgcgtgca ttgccatttg agtgtgcagg cagtgcgacc gcaatcctcg
    39421 ttcggtgtcg tacagcgcaa cgccggtttc gtcgcaagaa gattcgattc ctaataccaa
    39481 catggtctga tgccgttaaa aactgaaaaa cgtattctag cgaatttcgg cacgactgcc
    39541 gtatccccaa aacggaacat gccgtctgaa gcccttcaga cggcatcgtc gcaccgtatc
    39601 aaagcgttcc gtaagaatgc agcccgctca aaaacatatt cacgccgata aaggcgaatg
    39661 cggttacgaa caaaccgata atcgcccacc acgccagcac tttgccgcgc cagcccgcca
    39721 ccagccgcaa atgcagccac acggcgtaat tcaaccaaac gatgaacgcc caagtctctt
    39781 tcggatccca gctccaatag cgtccccaag cgtccgccgc ccacaacgcg cccaaaatgg
    39841 tggcaatggt aaagaacaaa aagccgacgg caatcgcctt atacatcacc tcgtcgatca
    39901 atgccgacgg cggcagccac agttttccgc ctttttcttc cgcacgcagg gaaaccagtt
    39961 cggcaatacc gagcatcgcg gaaatgcaga acgcgccgta accgataaag tttgccggaa
    40021 cgtggatttt catccaccag gactggagcg cgggaatcag cggctggatg gtatgcgcct
    40081 cacgcgacac gctgtaccac aacacaaagc cgaccacgac cgccataaag ccgaacacga
    40141 agccgcccaa tttctgcacg gcaaatttac cctcgtaata caaatacatc agcgcggtaa
    40201 tgaccaaaaa cagaatgaac acttcataaa ggttggacac ggggatatgc cctgcatcgg
    40261 gacggagcag atagctttcg tgccaacgta ccagcagacc ggtaaagcct gctacggcag
    40321 acacccatgc aaacacggtt cccataccca acagcgtgtt ggtcggcaca tttttcacgc
    40381 ttgccaaaac cgcgcccgaa atataggcga acagggcgaa gaagacaaaa gcacactgcc
    40441 acatgatcgc cgattggctg ctgaggaaat accgcaacag gaaaatctct gccgatttaa
    40501 tgttgccgcc atacagaccg acggcggcat aggcaagcaa tacgcttaaa ggaacaaacc
    40561 agcgcatcgg tttgaaaaac caacccaaaa acacggcaat accggcactc gcccacaaca
    40621 tgaccgtttc gtaaatgtcc atatgcatac cggaacgggt ctgcacgaaa accgtagccg
    40681 caaaaaccag cacggcaaat acccaatccc aaagattcag attgcggatc aaagatttct
    40741 gaatcagcag ctcgtgttcc ggaagggttt tatagtgttc agtcatgatt caagtccttg
    40801 ccgagccgtt gcaggctctc gacgtgtttt ggaaattcct tctgcaaatc ccgttcgctg
    40861 cgggccgaag acatagcaaa acggattttg ccgtctgaaa acaataccca cgcccgtttt
    40921 tcgcgcacat aaaacataaa taccgtaccc aaaaccaaca ataccgagcc gagatagacc
    40981 aaaagcgcac ccggcgaacg ggtcatctgc aaacctgagg aacgcacctc ggaaaacccg
    41041 tcaagctgga gcagcatagg cgcgggatat tccgtcagcc ccgtataggc atccatactg
    41101 tgcagcagga aacggttccg cgcttcatcc tgccgccatt cggacaagcc gtaccggcgt
    41161 atggtttcat ccaaagcagc gttcatcacg ccgtaaagca tttcgtagaa atagccctgc
    41221 atcttatcct gctgcccttt cgggatattg gacgtaataa attcgtccaa tcccaaatag
    41281 cctttttgcg caaagatatt cagcgtgttt tccgcagcca gcatgaattg ttcgcggatt
    41341 tcggcaggtg cgtctttggt tgcgtcggca accagacgtt tgcgcccttc cccatctttc
    41401 aaaaactcac gcaatgccat aaaggtgtcc gctttcaact gcttgtccaa ggggatacgc
    41461 agccagcggt attgctgctg caagccgctg cgcgtgccgg tcagccaaaa ataatctttg
    41521 tcctgcaaaa tcggcagcat atagttttta tattcgaccg cctgccctgc cgcatcacgg
    41581 atgcggtaca cgatggaagg gccgatattg gtgtattttt taccttcctg agtaacggcg
    41641 cggacatcgt tcagagtgga tttcaggctt ttttcccgtt ccgcaccctc gctcatgtcc
    41701 tccacattca tagaagtgaa ctgatcgaac tcaagacgat atttgtgttt gccgatttcc
    41761 aacggaaact ggtgtatgga ggttgccttc aacacgacag gttcgcgcga agcatccctc
    41821 aaattccacg ccttgaatgt caaatccgaa ccgccgtcgg caaaactcgc ctgataaatc
    41881 gtgatgccgt gcaaggtcaa aggatggttc acgcggatgg tgcgctcgag tttctcaccg
    41941 gttgccttgt ccgttacttc aatatcgctg gcaaaatcgc gcggcatacc cgtattgtaa
    42001 aaatcgatat ggaatttttt cagtttgact tcaaaaggca agtcctgaac caacatcccg
    42061 ttgtcggcat tcaggaaaac cacatccgca ctttgcccct cggaaatatt gacgttgccc
    42121 ctaaatgaga gattggacgc acccaaaata ctttcgggct tgaaatcctt ggcataaacc
    42181 gcctgattgt ccggaacaat ccgaccggcc agcataccca gcttcagcag caggttactg
    42241 tctatcaacc cgcccaggca aatgacaatc aaagcaacat gggcaaagat atagccccat
    42301 ttgttcatcg tgcctttttt ggcggcaatc agaaccgacc cgtcctcacg gctgacggtt
    42361 tttccctgaa aaccccgcac ctccagataa cgtttggcaa cttcgggggc aatttttaca
    42421 tccaacagcg aagaatggcg catcgccgcc agagattttt ctttaacctt ttcccggaaa
    42481 gacttcattt cgcgccaaaa cggcggaacg ttacggatta aacacaaact ggtagaaacc
    42541 accagaaaca tcatgataac gacaaaccat gccgaagcat agacatcgta caaacccaaa
    42601 aaatcaaaaa tccgagtcca aaacggtccg aatttgacca aataatccgt ctgcggctgg
    42661 ttttgctgta acaccgtgcc gataaccgat gcaataccca gcagactgag caaagcgacc
    42721 gcaaagcgca tggagctgaa aaaagcgaac cacggacggg aaagaagtgt gggggatata
    42781 cgggatttac tcattgtgtg tttattccgc catcaggaat atgggaaagc agaattgggc
    42841 agacagaaaa caacgtcccg attctactgt cttgatgctt tttatttcaa gacaatgaag
    42901 acagcctgca tcgattccaa cggttgcgat tgaaaaaact tatcgcaaaa ttttcagaag
    42961 ccgtctgaaa accttttcag acggcttctg aagcagaccc ttggatgatt aacgcaaacc
    43021 ctggataaag ttagcaactg cttttaaatc ttcttcggac atacggtttg caatatcttc
    43081 catgatggta tttttacgct gaccggactt gtaggcattc atctgttcaa caatatatgc
    43141 ctggtgctgg ccgcccaaac gcggataagc ctgaatttcg cttccgcctc ccggcatacc
    43201 cgcaccgctc ggaccgtggc aggacataca cgccggcact tttttatcgc tcaaaccgcc
    43261 gcgatagatt ttcgcgccca attcgggatt ttccttagga ttggcttcgc cggatttggg
    43321 ctgctgtttg gcatagaatg cggatacgtt caaaatatcc tgatcgctca aattcattac
    43381 caccggtttc atcacagctg ccgaaccgtg ggtgcgtttg ccgtcgcgga tgccgatagt
    43441 ttgatgatag atgtaagcag tatgctgtgc cgccaaacgc ggatacatcg caatgccgct
    43501 gttaccgtct gctgcatggc aagccgcaca aaccgttgcg gcaacctgtt tgcctttttc
    43561 cacgtctgct ttgggagatg cggaaaccgc accggcagcc aaaacaaagg ccaataaagt
    43621 caatcgtctc atggagtgct cctgattaca gcattggata acgcaacaat gctctttttt
    43681 atattcaaat atgggatttt tgacccgatt aaaaccgatg attctgtaaa cgtgttattc
    43741 tatactaaat ttacattaaa ttaccactgt gtttcacata aaaccaaccg catatttttg
    43801 ctgtcggaca aacggcggcg gaaaacaagg atatgcccat gaaccttttt caaaacgcca
    43861 aattcttcac gacggtaaac catctcaaag acctgccgga cacaccgctc gaaattgcct
    43921 ttgtcggcag gagcaatgcc ggaaaatcca gtgccatcaa taccctgacc aaccatgtcc
    43981 gtcttgccta cgtttcaaaa acaccgggac ggacacagca tatcaacttc ttcgagctgc
    44041 agaacggcaa ttttatggtc gatttgcccg gctacggtta tgcccaagtc cccgaagcgg
    44101 tacgcgcaca ttgggtcaat ctgctcggcg actacctccg ccatcggaaa cagcttatcg
    44161 ggctggtttt gattatggat gcccgccatc ctttaaaaga actcgacatc cgtatgctgg
    44221 actttttcca cacgaccggc agaccggttc acatcctgct gtcaaaagcc gacaaattat
    44281 ccaaaaacga acagataaaa accctgtccc aagtcaaaaa actgctcaaa ccttattccg
    44341 acaggcaaaa catcagcgta cagctgtttt ccagcctgaa aaaacaaggt attgacgagg
    44401 ccaaccgaac tgtcggaagc tggtttgacg cagcagatgc cgccgcttcc tctccagagg
    44461 aaaactgacc tcaattatac ggaaaccgta ttccccccac ttgaccggcc gcaaacattt
    44521 aaaaaattgc cactgccaaa tctaaaatgc cgtctgaaag acttttcaga cggcattttg
    44581 cggagtcttt aaaacaggga atccaactgc tgctgtttgg aatccgtatt gctcggaagc
    44641 accggcgttt cctgtacgtc ctgacgcgtt tcgtcagacc ttccctgctg ttcgttttct
    44701 actgccgctt catcatcttc ttttgcccgt cgggaaggtt gcggcgcaat accgctgttg
    44761 tccagcatca agcccggatc ggttaccata cgttccttca tatagtattc gccattgctg
    44821 ctgaccacac cttcaggcat tttcatccct ttgccctgct ttcctttcaa cgcaaaacgc
    44881 atatagtcca cccaaaccgg caccgcaatc gtaccgccgt agccggcacg ccccatactc
    44941 ttaggtttgt cgaagccgat atatacggca gtaaccacat cagggttaaa accgacaaac
    45001 cacgcatctt tattgtcgtt ggtcgtaccc gttttaccgg caatatccgt tcttcccaac
    45061 gcagctgccc cccttgccgt accgacacgg accacatcct gcataatctt atacataata
    45121 taggcattgc gcggatcgat tgcctgaggc gcattttgcc ctgccaccaa aggttgcatt
    45181 tgggcgcgca acctgccgtc tctgtcataa atcttatcga tcacgtgcga agaaacccta
    45241 tatccgccgt tcgcaaatac actatatgcc tccgccactt tcaacggcgt cgtctcgccc
    45301 gtacctaaag ccatagacag gcttgccggc agctcggacg gcctgaagcc gaaacgccgg
    45361 atatactgtt gcgcgtaacc gacaccgata gacatcaaaa tacggatgga aaccatattc
    45421 ttggaagccg tcagagcctg tctcaaagta atgtagccgg aatatctgcc gtctgaattt
    45481 ttaggtgtcc aaaccgaacc gttcggccct ttccccggca gggaaatcgg cgcatcgtta
    45541 accactgtgg acgcggtcat ccccttagat aatgccgccg aatagacaaa cggcttaaag
    45601 gtcgaacccg gctgccgcat tgcctgaacg gcacgattga atgttttgct gtgaaaatca
    45661 taaccgccga ccagcgcgcg cacagctccg gtttttgcat ccagcgaaac caaagccccc
    45721 tgcagcaacg gctcttgaac caccgcccaa cgcccgccgt tgtttttgac acggatgacc
    45781 gcgcccctgc ggatacggtc ctcccccatt ttctcattat cgaccgctcg ggccgcaaaa
    45841 cccaaggcgc gcctgtcaag cgcaacccgc ctgccgccgg gcagctgtat gacgacattt
    45901 ttctttttag taacatccaa cacaacggcg ggaaccattt tatcgacggt atagagtccc
    45961 gacagatact ggctgacagt ctcctcgaca tcttcactct tactcaaatc gatatagttt
    46021 tccgcaccgc ggtagctgct gccgcgatcg aaattccgta gagccttgcg caatgcctcg
    46081 gttgccgcct tctgatgatc ggtgcggacc gtggtataaa ccttaaaacc ctgcgtatag
    46141 gcatcttcac catatttctc atacagttcc cgacgcacca tttccgccac atataaagca
    46201 ctctgatcga ttttccgaac aaaccgctca taatgcagtt cctcattcaa tgcctgatcg
    46261 cgctgttgca cggtaatcat cttctcctcg agcatattgt tcaaaatata cttctggcgc
    46321 aacttggcac gctccggatt aacaatcgga ttataggcag acggagcctt gggcagtccc
    46381 gcaagcatgg cggcttccgc caaagtcaaa tctcggacat tcttattgaa atagatttgc
    46441 gcggcagatg caaaaccata ggcgcgctga ccgaggtaaa tctgattgaa atacaactca
    46501 aggattttgt ctttgcttaa agactgctcg attttatagg caagcaacac ctcattgaat
    46561 ttgcgtgtga acgttttttc actgctcaaa taaaaatttt tcgccacctg ctgtgtaatc
    46621 gtactcgcac ccgactgcac gctgccggac acgacattgc cgacggcagc gcgggcaaca
    46681 ccccaaacat ccacccccca atgccggtaa aagcgtttat cctcggcggc aataaccgca
    46741 ttccgcaaca cctcggggaa atcgccgatt tttgtaaatt cgcgccgctg ctccccatac
    46801 ataccgatga cttctccatc cgccgaataa atagtcaacg gcattttagg ctggtaatgc
    46861 tgcaaagaat ccaaagacgg cagtttcgga tacgttacca aaatagcaat ggcaaccaga
    46921 cccactccaa atacacaaaa accaaaaaac aaaccaaaac aagtagttaa aatcttttta
    46981 atcatagctg aataataatt taccattatt ggtattaaat aaagtaaaat agcaaccgat
    47041 ttctacaaag cacggtttca atgtgcaaag aacaaggaat ccattacgga taccgaaacg
    47101 gttactcact gtacaaataa agcaggaact ttcatcatgc gcttgtttaa aagcttgaaa
    47161 aaccctaaaa aaacagatgc caagctccct aaaaaatctt cgggactcaa taaccgcgcg
    47221 gcaatcggca tcgatatcga ccagcattcc atcaaaatgg tacaattgtc aggacgtagt
    47281 ttaaaccaaa ttcaattgga aaaatacgtc attgccaaat taccaaagaa tatcattcaa
    47341 ggcaataaag tccaaaatta cgatcaactt gttacatatt tgcaacaagc ctatgccaaa
    47401 ctgggtactt cgtgcaaaaa catcgtcgcg tccgtcccgc aaaatctggc aaccatcgaa
    47461 caattgacct acacggccaa agatgcagaa ttggacctgc aggggttcgt ggagtcctcc
    47521 atctccgaag ccagctcgat atcgctcgaa gaagccaatt acgactatca ggtcttgtcc
    47581 caatcggccg tcggcgaagc tgttttgtcc gtcgcatcga gaaaggatga aatcgaaccc
    47641 ctgattgacg cattcaacgc agccggtatg aaattatccg cgcttgatgt ggacattttc
    47701 ggacaataca acgcctacgc gctgtggata aaccattttg cccccgagct tgcggacgaa
    47761 aaagtggcca ttttcggcgt atatgccgca cagacctacg ccctggtcat ccaagacgga
    47821 aaaatcctat acaaacagga aacctccgtc agcgaagaac agctcaacca actcatccag
    47881 cgcacctatc aggtaacagc agaaaaagcg gaagagatca tcaactcccc gcaaaaacct
    47941 tccgattacc aagaaagcgt ggcaaactat ttcaaccagc agattaccca agaaatacaa
    48001 agggtcttgc agttttatta caccacgcag accgcagacg atatgaccga catcaagcat
    48061 atcctgctga cgggggaagc ggtgcgccag aaaggcatcg cccaaaccgt cgcctcgcaa
    48121 accaatgcgg atgtacaatg cgtccatccc gcgcgttatt ttgcgaacga cctcaaaaca
    48181 gacgaacaac aattcgaact tgatgcgccg acactgacca aggcgttcgg tttggcggta
    48241 cggggattat aattatgaac aatttaatca aaatcaacct cctcccctac agggaagaga
    48301 tgaacaaacg caaacagcag cagtttaaaa cgctgatgta cggcgccgtg ctgacgggcg
    48361 ttgccgccgt tgcggcaacc tacctgttta tcgacaatat gatcaataaa cagtcggaaa
    48421 aaaacacgct gctggaaatc tccatcgcac acttggatac cgagctgtcg aaaatacaaa
    48481 agctcaaaca ggaaaaagat gccttcctga ttaagaaaaa caaaatcggg gagctccaga
    48541 tcaaacgcct ccaagccgca aaaatcctcg acagcctgaa tgaggccgtc cccggaagca
    48601 cctacctaac ctcgctggat gccgttaccg ccgactctta tcggctcagc ggcaggacat
    48661 ccagcgacaa ccgcgttgcc gccatgatga gggcgatgcc caataccggc atattcaagc
    48721 aacccgaatt gttaagcatc aagaaaaaca attcgcatca agaatttacc cttcaggcaa
    48781 cattacaacc tatcgtaaag gcggccgaat ccaaagagaa tccggcttcg ggaaacgcac
    48841 aggaggcaaa ctgaatggct tctaaatcat ctaaaaccaa cttggatctc aacaaccttc
    48901 acctgctcaa ccttcctgcc aggcttttta tcgccctgct ggtcgttgcc gccgtgctgg
    48961 ggctcggtta tgccggattg ttcaaaagcc agatggaatc ccttgaggaa tatgaagcaa
    49021 aagaaaccga actgaaaaac acctacaaac agaaaagtat cgacgcggcc agcctgaaca
    49081 accttaggga cgaacttgcc tcaatccgct ctgccttcga tatcatgttg aaacagctgc
    49141 cgacagatgc agaaattccc aatttggttc aagagcttca tcaggcgggt tcgagcaacg
    49201 gtctgcgctt ggacagcgtt atgccccaac ctcccgtaga tgacggtccc atcaaaaaat
    49261 taccctattc catttccatt accggaaatt acgaacagat cagccaattt acccgcgatg
    49321 tcggcagtct ctcccgaatc attacccttg agtcgctgaa aatcgcccaa tctccggaaa
    49381 acggcggcaa tcctgacggc aagagcagta tcctgaacct cagcgccatt gccaccacct
    49441 accaagcaaa atccgtagaa gagcttgccg cagaagcggc acaaaatgcc gagcaaaaat
    49501 aacttacgtt agggaaacca tgaaacacta tgccttactc atcagctttc tggctctctc
    49561 cgcgtgttcc caaagttctg aagacctaaa cgaatggatg gcacaaacgc gacgcgaagc
    49621 caaagcagaa atcatacctt tccaagcacc taccctgccg gttgcgccgg tatacagccc
    49681 gccgcagctt acagggccga acgcattcga cttccgccgc atggaaaccg ccaaaaaagg
    49741 ggaaaatgcc cccgacacca agcgtattaa agaaacgctg gaaaaattca gtttggaaaa
    49801 tatgcgttat gtcggcattt tgaagtccgg acagaaagtc tccggcttca tcgaggctga
    49861 aggttatgtc tacactgtcg gtgtcggcaa ctatttggga caaaactacg gtagaatcga
    49921 aagcattacc gacgacagca tcatcctgaa cgagctgata gaagacagca cgggcaactg
    49981 ggtttcccgt aaagcagaac tgctgttgaa ttcttccgac aaaaacaccg aacaagcggc
    50041 agcacctgcc gcagaacaaa attaagaaga ggattactcc attatgaata ccaaactgac
    50101 aaaaatcatt tccggtctct ttgtcgcaac cgccgccttt cagacggcat cggcaggaaa
    50161 cattacagac atcaaagttt cctccctgcc caacaaacag aaaatcgtca aagtcagctt
    50221 tgacaaagag attgtcaacc cgaccggctt cgtaacctcc tcaccggccc gcatcgcctt
    50281 ggactttgaa caaaccggca tttccatgga tcaacaggta ctcgaatatg ccgatcctct
    50341 gttgagcaaa atcagtgccg cacaaaacag cagccgtgcg cgtctggttc tgaatttgaa
    50401 caaaccgggc caatacaata ccgaagtacg cgggaacaaa gtttggatat tcattaacga
    50461 atcggacgat accgtgtccg cccccgcccg cccagccgta aaagccgcgc ctgccgcacc
    50521 ggcaaaacaa caggctgccg caccgtttac cgagtccgta gtatccgtat ccgcaccgtt
    50581 cagcccggca aaacaacagg cagcggcatc ggcaaaacaa caggcggcga caccggcaaa
    50641 acaaaccaat atcgatttcc gcaaagacgg caaaaatgcc ggcattatcg aattggcggc
    50701 attgggcttt gccgggcagc ccgacatcag ccaacagcac gaccacatca tcgttacgct
    50761 gaaaaaccat accctgccga ccgcgctcca acgcagtttg gatgtggcag acttcaaaac
    50821 accggttcaa aaggttacgc tgaaacgcct caataacgac acccagctga ttatcacaac
    50881 aaccggcaac tgggaactcg tcaacaaatc cgccgcgccc ggatacttta ccttccaagt
    50941 cctgccgaaa aaacaaaacc tcgagtcagg cggcgtgaac aatgcgccca aaaccttcac
    51001 aggccggaaa atctcccttg acttccaaga tgtcgaaatc cgcaccatcc tgcagatttt
    51061 ggcaaaagaa tccgggatga acattgttgc cagcgactcc gtcaacggca aaatgaccct
    51121 ctccctcaaa gatgtgcctt gggatcaggc tttggatttg gttatgcagg cgcgcaacct
    51181 cgatatgcgc cagcaaggga acatcgtcaa catcgcgccc cgcgacgagc tgcttgccaa
    51241 agacaaagcc ttcttacagg cggaaaaaga cattgccgat ctgggcgcgc tgtattccca
    51301 aaacttccaa ttgaaataca aaaatgtgga agaattccgc agcatcctgc gtttggacaa
    51361 tgccgacacg accggaaacc gcaacacgct tgtcagcggc aggggcagcg tgctgatcga
    51421 tcccgccacc aacaccctga ttgttaccga tacccgcagc gtcatcgaaa aattccgcaa
    51481 actgattgac gaattggacg tacccgcgca acaagtgatg attgaggcgc gtatcgtcga
    51541 agcggcagac ggcttctcgc gcgatttggg cgttaagttc ggcgcgacag gcaggaaaaa
    51601 actgaaaaat gagacgagcg cattcggctg gggcgtgaac tccggcttcg ggggcggcga
    51661 taaatgggag gcccaaacca aaatcaacct gccggttgcc gctgccgcaa acagcatttc
    51721 gctggtgcgc gcgatttcct ccggcgcgtt gaatttggaa ttgtccgcat ccgagtcgct
    51781 ttcaaaaacc aaaacgcttg ccaatccgcg cgtgctgacc caaaaccgca aagaggccaa
    51841 aatcgaatcc ggttacgaaa ttccttttac cgtaactaca gcctcgggcg gcggcaactc
    51901 taccaacacg gaactcaaaa aagccgtctt ggggctgacc gttacgccga acatcacgcc
    51961 cgacggacaa atcatcatga ccgtcaaaat caacaaagac tcgcctgcac aatgtgcttc
    52021 aggcaacaac acaatcctat gtatttcgac caaaagcctg aatacgcagg ctatggttga
    52081 aaacggcggc actttgattg tcggcggtat ttatgaagaa aacaacggca atacgctgac
    52141 caaagtcccc ctgttgggcg acatccccgt tatcggcaac ctctttaaaa cacgcgggaa
    52201 aaaaaccgac cgccgcgaac tgctgatttt cattaccccg aggattatag atacggccgg
    52261 caacagcctg cgctattgat gcgtcaaaat aagggcatat gttttacagc atatgccctt
    52321 tctttatgct ttttgccgcg accgaaatgc cgtcattccc gcgcaggcgg gaatccaact
    52381 tgtccggttt cggttgtttt tcgtctcgta acttttgagc cgtcattccc gcgaaatcgg
    52441 aaatccagtc cgttcagttt cggtcatttc cgataaattc ctgttgcttt tcatttctag
    52501 attcccactt tcgtgggaat aacggcggaa gggataaatc ctcgcaatcc aaagcctcgt
    52561 catttccaca aaaaacagca acccgaaaca ccccgtcatt cccgagcagg cgggaatcta
    52621 ggtctttaaa cttcggtttt tttccgataa attcttgcct cattgaaatt ctagattccc
    52681 gctttcgcgg gaatgacggg atatgggttt ccgtgcggac gtgttcggat ttccgcctgc
    52741 gcgggaatga cgattcgggt attactgacg attcatcggg ccgtagggtg ggcttcagcc
    52801 cactaatttc accgcatcaa gggttttggg gaataggcgt aactcggcgg tttcggtttt
    52861 tggcgaagtt ggttggaatc ggcgggctga agcacaccct acagtccacc ctacaaccca
    52921 tcttacgcat ccgccttacg catttatcct tcgcctgccg atatgccgtc attcccgcac
    52981 aggcgggaat ctagaaccgc aacgcggcag gaatctgtcg gatacggctg aaaccgaacg
    53041 gact
//
LOCUS       DBJGGI010000007        41609 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.007, whole genome shotgun sequence.
ACCESSION   DBJGGI010000007 DBJGGI010000000
VERSION     DBJGGI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 41609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41609
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.007"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..779
                     /gene="pglE"
                     /locus_tag="ACSIGJ_000411"
     CDS             <1..779
                     /gene="pglE"
                     /locus_tag="ACSIGJ_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(galactosaminyl)2-N,
                     N'-diacetylbacillosaminyl-alpha1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglE"
                     /protein_id="HHQ1329380.1"
                     /translation="YQDNQGLSEARNTGIKNSNGKYIAFIDSDDFINCQVLLDFLGKD
                     DSDMPDVVFLNAVKYDKGRVSYFGEDYQPEKILNQSKVEVLKGLCRFRKFPGSAWNKI
                     IKRELIIREKLFFEKGIYSEDIEWSMRLFNAATTFSYLDGCYYYYRQGRKDSITGTVS
                     EKGIKSLLYILEKNAKMEFNRDISSYLYSFLSYEYLVLLFIMTSKNIACDSDIKRRAY
                     HLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKAIRGNI"
     gene            820..1350
                     /locus_tag="ACSIGJ_000412"
     CDS             820..1350
                     /locus_tag="ACSIGJ_000412"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HHQ1329381.1"
                     /translation="MNAIRTFQNRMPEIHETCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDDVMIGAGSLVPPRKRLEGGYLYIGSPVRQVRLLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(1518..3113)
                     /locus_tag="ACSIGJ_000413"
     CDS             complement(1518..3113)
                     /locus_tag="ACSIGJ_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HHQ1329382.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVEDI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFGIIKGINNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATMRMVGPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHNRELAEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(3220..3615)
                     /gene="hisI"
                     /locus_tag="ACSIGJ_000414"
     CDS             complement(3220..3615)
                     /gene="hisI"
                     /locus_tag="ACSIGJ_000414"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HHQ1329383.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDTIVMLIAQNGGIACHTGRES
                     CFYKKWNGGAWETADAVLKDEKEIYGSTH"
     gene            complement(3646..4413)
                     /gene="hisF"
                     /locus_tag="ACSIGJ_000415"
     CDS             complement(3646..4413)
                     /gene="hisF"
                     /locus_tag="ACSIGJ_000415"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HHQ1329384.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLINEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEW
                     AVEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRTMREAGIEVRL"
     gene            complement(4426..5163)
                     /gene="hisA"
                     /locus_tag="ACSIGJ_000416"
     CDS             complement(4426..5163)
                     /gene="hisA"
                     /locus_tag="ACSIGJ_000416"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HHQ1329385.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPADTALHWFEQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGMRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDLVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQSVRIPVIASGGLTGLDDIPALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(5196..5834)
                     /gene="hisH"
                     /locus_tag="ACSIGJ_000417"
     CDS             complement(5196..5834)
                     /gene="hisH"
                     /locus_tag="ACSIGJ_000417"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HHQ1329386.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTKIFLSGDPDRVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFARDLRDPQGCRLKVPHMGWNTVRQTQNHPLFQGIPQNTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(5964..7466)
                     /gene="pta"
                     /locus_tag="ACSIGJ_000418"
     CDS             complement(5964..7466)
                     /gene="pta"
                     /locus_tag="ACSIGJ_000418"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HHQ1329387.1"
                     /translation="MAKVLVVPVSAGLNTSAAAQAFAKALDAQVFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NTDADALAHQLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQAGKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7651..8709)
                     /gene="fbpC"
                     /locus_tag="ACSIGJ_000419"
     CDS             complement(7651..8709)
                     /gene="fbpC"
                     /locus_tag="ACSIGJ_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HHQ1329388.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIIGASGCGKT
                     TLLRCLAGFEQPDSGEISLSGKTIFSKNTNLPVRERRLGYLVQEGVLFPHLTVYRNIA
                     YGLGNGKGRTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCRLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPAASIHAVVLKTTPKARHTEISLRAGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8730..10247)
                     /gene="fbpB"
                     /locus_tag="ACSIGJ_000420"
     CDS             complement(8730..10247)
                     /gene="fbpB"
                     /locus_tag="ACSIGJ_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HHQ1329389.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLTAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LILGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTADDVHTLSTAVWEYTSDAQYAAAAPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(10315..11310)
                     /gene="fbpA"
                     /locus_tag="ACSIGJ_000421"
     CDS             complement(10315..11310)
                     /gene="fbpA"
                     /locus_tag="ACSIGJ_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HHQ1329390.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYVPT
                     SGAFLEQIVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLAGKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(11562..12042)
                     /locus_tag="ACSIGJ_000422"
                     /pseudo
     CDS             complement(11562..12042)
                     /locus_tag="ACSIGJ_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMP19 family protein"
     gene            complement(12100..13476)
                     /gene="argH"
                     /locus_tag="ACSIGJ_000423"
     CDS             complement(12100..13476)
                     /gene="argH"
                     /locus_tag="ACSIGJ_000423"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HHQ1329391.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDQRLAEWDIQGSLAHAQ
                     MLTRSGVLSENDLTDIRRGMSEILEEIRSGKIEWPLDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQNLIQNLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDFERMADCRKRVNRMPLGAAALAGTTYPIQREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPGNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEALQGFSKLISDDVYGVLTPEGSLNARNHLGGTAPEQVRLQV
                     KRWREMSA"
     gene            complement(13495..14364)
                     /gene="galU"
                     /locus_tag="ACSIGJ_000424"
     CDS             complement(13495..14364)
                     /gene="galU"
                     /locus_tag="ACSIGJ_000424"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HHQ1329392.1"
                     /translation="MKPIKKAVFPVAGMGTRFLPATKANPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETKLEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEPSQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(14392..14991)
                     /gene="rdgB"
                     /locus_tag="ACSIGJ_000425"
     CDS             complement(14392..14991)
                     /gene="rdgB"
                     /locus_tag="ACSIGJ_000425"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HHQ1329393.1"
                     /translation="MSEKPEKIVLASGNAGKLKEFGNLFKPYGITVLPQSPFGIPECP
                     EPYPTFVENALAKARHAAGHSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCSYVCVLVFVRHKDDPRPIIAEGIWHGQWHDTPLGQNGFGYD
                     PYFYLPEHGKTAAELDSEVKNRESHRAQALADLIRKLAL"
     gene            complement(14984..15217)
                     /locus_tag="ACSIGJ_000426"
     CDS             complement(14984..15217)
                     /locus_tag="ACSIGJ_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO_0222 family membrane protein"
                     /protein_id="HHQ1329394.1"
                     /translation="MNRRKIYLLSVALFTLAFMLPVLLGAYLLTAGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLRAAPSSSQGNRYV"
     gene            complement(15325..15858)
                     /locus_tag="ACSIGJ_000427"
     CDS             complement(15325..15858)
                     /locus_tag="ACSIGJ_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HHQ1329395.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RIEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGGAE
                     EAKKVIKESIERWNKQA"
     gene            complement(15960..16418)
                     /gene="nudB"
                     /locus_tag="ACSIGJ_000428"
     CDS             complement(15960..16418)
                     /gene="nudB"
                     /locus_tag="ACSIGJ_000428"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HHQ1329396.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLEDGQLQDRHDSTVYEIYHHWRHRYPKGVFENREHVFR
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            16629..16705
                     /locus_tag="ACSIGJ_000429"
     tRNA            16629..16705
                     /locus_tag="ACSIGJ_000429"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16663..16665,aa:Pro,seq:ggg)
     gene            16968..18854
                     /gene="mafB"
                     /locus_tag="ACSIGJ_000430"
     CDS             16968..18854
                     /gene="mafB"
                     /locus_tag="ACSIGJ_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HHQ1329397.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDPFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGAMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFTAVIPVKGIGAVRGKYGLGGI
                     TAHPVKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAHAQREWENKTGLDFNHF
                     IGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVEIKKPDGSWEVKTKKGG
                     KVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNKWQGTSKSGIKIEGFTEPNRTAY
                     PIYE"
     gene            18885..19304
                     /locus_tag="ACSIGJ_000431"
     CDS             18885..19304
                     /locus_tag="ACSIGJ_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329398.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            19382..19465
                     /locus_tag="ACSIGJ_000432"
                     /pseudo
     CDS             19382..19465
                     /locus_tag="ACSIGJ_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245501.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 17 domain-containing
                     protein"
     gene            19462..19704
                     /gene="imm31"
                     /locus_tag="ACSIGJ_000433"
     CDS             19462..19704
                     /gene="imm31"
                     /locus_tag="ACSIGJ_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm31 family immunity protein"
                     /protein_id="HHQ1329399.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            19782..20027
                     /locus_tag="ACSIGJ_000434"
     CDS             19782..20027
                     /locus_tag="ACSIGJ_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HHQ1329400.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGKDKI
                     PSYTWHHHQDTGRMQLVPEWEHSKTGHIGGTAMGKGK"
     gene            20030..20416
                     /locus_tag="ACSIGJ_000435"
     CDS             20030..20416
                     /locus_tag="ACSIGJ_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329401.1"
                     /translation="MWKIYKENSTDLNFAIGSIYCQAINLTEFKMWVEKIIREVDLDE
                     IPNYFFDLIDFQSLYDLIDIIGFVPENNLSKNQDNALTGIAFLRGIDVYDPPISKEKA
                     LKALEKHPEIYQKFQHFFPFVELPPL"
     gene            20503..20634
                     /locus_tag="ACSIGJ_000436"
                     /pseudo
     CDS             20503..20634
                     /locus_tag="ACSIGJ_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            20637..21027
                     /locus_tag="ACSIGJ_000437"
                     /pseudo
     CDS             20637..21027
                     /locus_tag="ACSIGJ_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21067..22524)
                     /locus_tag="ACSIGJ_000438"
     CDS             complement(21067..22524)
                     /locus_tag="ACSIGJ_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HHQ1329402.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHALATL
                     SLGGFSTHDASIGYYNSPLIEAVIIVFTIFGGINFASHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGHYAGFGESLRYTAFNFVSIGLANGLSNTDFAQWPLLIS
                     LWMFFLANILASSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(22539..23369)
                     /locus_tag="ACSIGJ_000439"
     CDS             complement(22539..23369)
                     /locus_tag="ACSIGJ_000439"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HHQ1329403.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLYLLAVGCGEGALKRSDTIEPILKHPDGGKMLDWL
                     RAQPLLIREGGRVMIHAGILPQWRIAKAESLAGEAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     HTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            complement(23446..23835)
                     /locus_tag="ACSIGJ_000440"
     CDS             complement(23446..23835)
                     /locus_tag="ACSIGJ_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HHQ1329404.1"
                     /translation="MSKTVIHTDKAPAAIGAYSQAVRAGGIVYMSGQIPLDPATMTVV
                     GNGDFHTEARQVFQNLQTVAEAAGGSLDDIVKLNAYLTDLGNFAVFNEVMAEFITEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            complement(23982..24509)
                     /gene="nspA"
                     /locus_tag="ACSIGJ_000441"
     CDS             complement(23982..24509)
                     /gene="nspA"
                     /locus_tag="ACSIGJ_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HHQ1329405.1"
                     /translation="MKKALAALIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKQAPSTDFKLYSIGASVIYDFDTQSPVKPYF
                     GARLSLNRASAHLGGSDSFSKTSAGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNNVK
                     NVRSGELSAGVRVKF"
     gene            complement(24952..26127)
                     /gene="hemW"
                     /locus_tag="ACSIGJ_000442"
     CDS             complement(24952..26127)
                     /gene="hemW"
                     /locus_tag="ACSIGJ_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HHQ1329406.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYSLPNQTVQTALDDVQTAIATDATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGTLEGAGFIHYETSAFAKPTMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPGEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPAAKIMVQIETARQKGLLETDPTVFRPTEKGRLFLNDLLQCFL"
     gene            complement(26184..28655)
                     /gene="ligA"
                     /locus_tag="ACSIGJ_000443"
     CDS             complement(26184..28655)
                     /gene="ligA"
                     /locus_tag="ACSIGJ_000443"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HHQ1329407.1"
                     /translation="MNPTAQRIHELTDLLNRYAYEYYTLDAPSIPDAEYDRLFRELEA
                     LERNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPMLSLTNAFSPQDENGVFDHAEMYA
                     FDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVLVQAATRGDGTTGEDVTRNVKTV
                     SNIPLRLHGENVPELIEVRGEVLMLKADFAGLNKRQAENGQKPFANPRNAAAGSLRQL
                     DSRITAQRKLHFFPYSIARQQGGFEAEEHIQELAYFQELGFSLPNGNFGCFKNIGEVL
                     AFYEHMQQKRPELPYEIDGMVVKVNSLAQQRELGFISRAPRWAVAHKFPAEEALTIVE
                     AIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQDEVSRKDVRVGDTVVVRRAGDV
                     IPEVVRVIFERRPMQETAVAVSDGIGHQQDDLFAETPSAKQTESVPLHKPYRLPARCP
                     ICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFASRKAMDIDGLGEKQIEQLVAQDL
                     VRHFADLYRLDIPTLQKMKETADKGSSENENGDAETVSGDLSKYNTQNGKKQPTKWAQ
                     NILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGTLERVRRAPEPVLACLPDI
                     GTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPAAQYAGPQRWITRLPGFKI
                     SENKAQALWELAGQSIEGLQNDKALPADWQAWRSKAQNTALLENLKTFFAQMPSEDEA
                     AQGSDGINKAVAGKTFVLTGTLPTFKRDQAQALIEAAGGKVSGSVSKKTDYVVAGEAA
                     GSKLEKANALGVSVLSEAELLTLLG"
     gene            complement(28789..30075)
                     /locus_tag="ACSIGJ_000444"
     CDS             complement(28789..30075)
                     /locus_tag="ACSIGJ_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HHQ1329408.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKPAKPQDSAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQADAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(30269..30841)
                     /gene="ampD"
                     /locus_tag="ACSIGJ_000445"
     CDS             complement(30269..30841)
                     /gene="ampD"
                     /locus_tag="ACSIGJ_000445"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HHQ1329409.1"
                     /translation="MDNHAGAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLHVSSHFLIARDGETVQFVSCGDMAYH
                     AGASSFRGREKCNAFSIGIELEGCDFEPFAEAQYRSLETLLAALCRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIREKGFPVDRNAV"
     gene            30925..31920
                     /gene="mltG"
                     /locus_tag="ACSIGJ_000446"
     CDS             30925..31920
                     /gene="mltG"
                     /locus_tag="ACSIGJ_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HHQ1329410.1"
                     /translation="MLKKMLKWTAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIGHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLQIYQTAYKAMQRRLNEAWAGRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            31980..32600
                     /gene="tmk"
                     /locus_tag="ACSIGJ_000447"
     CDS             31980..32600
                     /gene="tmk"
                     /locus_tag="ACSIGJ_000447"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HHQ1329411.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEEVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQAREKDRFEQEQAD
                     FFMRVRGVYLDRAAACPERYAVIDSNRSLDEVRNSIEKVLDGHFGC"
     gene            32822..34102
                     /locus_tag="ACSIGJ_000448"
     CDS             32822..34102
                     /locus_tag="ACSIGJ_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HHQ1329412.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTGKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRYTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGRRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLGEWKL"
     gene            34509..35540
                     /gene="lpxK"
                     /locus_tag="ACSIGJ_000449"
     CDS             34509..35540
                     /gene="lpxK"
                     /locus_tag="ACSIGJ_000449"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HHQ1329413.1"
                     /translation="MPNFFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKWRADF
                     LSGKRQSEKLSVPVVVVGNIHAGGTGKTPIAAALVSGLQEKGVKVGIISRGYGRKSKA
                     VYVLNAASRAEDAGDEPLLLFRKTGAPTAVGSSRVEAGRALLAAHPELELIVADDGLQ
                     HYALQRDVEIAVFPAADTGRTDLDLLPNGNLREPLSRLESVDAVVVGGRAADGFMPSE
                     HLFGSRIEAGAVYRLNRPSEKLDISTLSGKRVAAVAGIARPQRFFDTLTHMGIRLDQT
                     VALPDHADIFNRDLPPADVVLVTEKDAVKFSDGICTDNVWVLPVCAIIEPDLAEFVLE
                     RLEGVPKAV"
     gene            35746..36279
                     /locus_tag="ACSIGJ_000450"
     CDS             35746..36279
                     /locus_tag="ACSIGJ_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HHQ1329414.1"
                     /translation="MKLKTLLLPFAALALCANAFAAPPGDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PEFTEELRRIICGGIVD"
     gene            36264..36371
                     /locus_tag="ACSIGJ_000451"
                     /pseudo
     CDS             36264..36371
                     /locus_tag="ACSIGJ_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            36501..36683
                     /locus_tag="ACSIGJ_000452"
     CDS             36501..36683
                     /locus_tag="ACSIGJ_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HHQ1329415.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALSEEELKA"
     gene            36680..37441
                     /gene="kdsB"
                     /locus_tag="ACSIGJ_000453"
     CDS             36680..37441
                     /gene="kdsB"
                     /locus_tag="ACSIGJ_000453"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HHQ1329416.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTACQAHGIEVVMTSNRHESGTTRLAEAAAALKLPPHLIVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAGHELHDFDELMNPNAVKVVLDKNGNAIYFSRAP
                     IPYPRDAMRAGKREMPSETAVLRHIGIYAYRVGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            37464..37979
                     /locus_tag="ACSIGJ_000454"
                     /pseudo
     CDS             37464..37979
                     /locus_tag="ACSIGJ_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            38136..38228
                     /locus_tag="ACSIGJ_000455"
     tRNA            38136..38228
                     /locus_tag="ACSIGJ_000455"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38170..38172,aa:Ser,seq:gct)
     gene            38502..39287
                     /gene="trpA"
                     /locus_tag="ACSIGJ_000456"
     CDS             38502..39287
                     /gene="trpA"
                     /locus_tag="ACSIGJ_000456"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HHQ1329417.1"
                     /translation="MSRIRQAFAALDGGKALIPYIAVGDPDIRTTLALMHGMVASGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PIHKMGYREFAQEAAKAGVDGVLTVDSPIETIDSLYRELKDNEVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLRQYIDIPIGVGFGISNAESA
                     RKIGRVAAAIIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            39324..40196
                     /gene="accD"
                     /locus_tag="ACSIGJ_000457"
     CDS             39324..40196
                     /gene="accD"
                     /locus_tag="ACSIGJ_000457"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HHQ1329418.1"
                     /translation="MSWLDKILPPKIKNRGKDGSSNVPEGLWRKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEEGREEVAGNVKPTDPLKFKDSKKYPDRLSAA
                     RKLTGEDDALVVMKGMMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAT"
     gene            complement(40256..40867)
                     /locus_tag="ACSIGJ_000458"
     CDS             complement(40256..40867)
                     /locus_tag="ACSIGJ_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HHQ1329419.1"
                     /translation="MRRAILLILTLTVGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAELPPLPDAHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCALSSIGFGNGKLSSYKVFGYGDTVNSRWIQPRNAEWKPIGGTLGRN
                     DALRAVLYQAFCEGGVPADTQGLVQRLKERAGRYAPSMKPHDK"
     gene            40975..41199
                     /locus_tag="ACSIGJ_000459"
     CDS             40975..41199
                     /locus_tag="ACSIGJ_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HHQ1329420.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMRQGEVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDSSEQDGVFTLVVKHK"
     gene            41230..>41609
                     /locus_tag="ACSIGJ_000460"
     CDS             41230..>41609
                     /locus_tag="ACSIGJ_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NMCC_0638 family (lipo)protein"
                     /protein_id="HHQ1329421.1"
                     /translation="MLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLFRSACVLTQGS
                     FEPVSKFAAVGHFVPVGKEDLSRLPPAVAEPDVQALWTLERDGGIYYLSLTRDSCSVK
                     AERADSAALLEHFSGLVRQPPKNA"
BASE COUNT         9198 a         9920 c        12448 g        10043 t
ORIGIN      
        1 tatatcagga taaccaagga ttgtcggagg cgagaaatac cggaataaaa aattcaaatg
       61 gaaaatatat agcatttatt gattcggatg attttattaa ttgtcaggtt ttgctggatt
      121 ttcttggtaa agatgatagt gatatgccgg atgtggtttt tttaaatgca gttaaatatg
      181 ataagggaag agtttcatat tttggcgaag attatcagcc tgaaaaaata ttaaatcaat
      241 ccaaagtcga agttttgaaa ggattgtgcc gatttagaaa atttccgggt tcggcgtgga
      301 ataagattat aaaaagagaa ttgattatta gagaaaaact gttttttgaa aagggaattt
      361 attctgaaga tattgaatgg tcaatgaggt tgtttaatgc ggcaacaact ttttcttatt
      421 tggacggttg ttattactat tatcggcagg gaagaaaaga ttctattacg ggaactgttt
      481 cggaaaaagg tataaagtca ttattatata ttttggaaaa aaatgcgaaa atggaattta
      541 atagggatat atcgagttat ctttattctt ttctttccta cgaatatctc gttttgcttt
      601 ttataatgac gagtaaaaat atagcgtgtg attctgatat aaaaagaagg gcatatcatt
      661 taaggtttat gctgttaaag tccaataagc tgatatataa gctgatattc ccgataatca
      721 cattattcgg ggtcgatatt acaggcagga ttttaaaagc aatcaggggg aatatttaat
      781 aaatccttta acaatatata ccttaccgaa ggaggaaaaa tgaacgcaat cagaactttc
      841 caaaaccgca tgcccgaaat ccacgaaacc tgcatgatag acgaggcgtg cgtcgtcatc
      901 ggcgaagtct cgcttgccga agatgtttcc gtgtggccgt gcgccgtgtt gcgcggcgat
      961 gtgaacagca tcaccgtcgg cgcgcgcagc aatatacagg acggcagcgt cctgcacgtt
     1021 tcccacaaaa ccgccgccaa acccgaaggg tcgccgctgg ttatcggcga agacgttacc
     1081 gtggggcaca aagtgatgct gcacggctgc cgaatcggta accgcgtctt ggtcggtatg
     1141 ggcagcaccg ttttggacga tgccgttatt gaagatgacg tgatgatcgg cgcgggcagc
     1201 ctcgttccgc cgcgcaaacg tttggagggc ggctatcttt atatcggttc gcctgtgagg
     1261 caggttcgtc tgctgaccga tgaggaaaaa gcctttttga aatattccgc cgcacattat
     1321 gtgaagctgt cgaaacagta cgggatgtga aatcacatcg gcgttcttgc gtcagtccca
     1381 aattcacgcg gttgggatgc ccgataacgg tatccgatgc gccttgattt tgaccggctg
     1441 cgtttgagtt gcaggcaaaa atgccgtctg aaagcctttt tttcggcttc ggacggcatt
     1501 ttattgccga tttcttttta gagtttgacc gaatgttcgc gcgtttcgtg gaacacgatg
     1561 tccggccaac gttcttgcgt caaccccaaa ttcacgcggt tgggggcgag gtaggcgagg
     1621 ttgccgcctg cgtcgatggc gaggttgcct gcgttggctt tttcaaattc cgccagtttt
     1681 ttcttgtcgt cgcacgatac ccagcgcgcc gaccagatgg atgcgctgtc gaacacggct
     1741 tccacgccgt attcgttggc gaggcgtgag gttacgactt caaactgcaa cacgccgacc
     1801 gcacccaaaa tcaaatccgc gccgctcatc ggtttgaata cttgaaccgc accttcttcg
     1861 ccgagttgtt gcaaaccttt ttgcagttgt ttgattttca gcgggttttt gatgcggacg
     1921 ctgcggaaca gttcgggtgc gaagaatggg atgccggtaa acgccagttg ttcgccttcg
     1981 gagaagctgt cgccgatttg gatgttgccg tggttcggga tgccgatgat gtcgccggcg
     2041 taggcttctt ccgccagttc gcggttgtgc gacatgaagg ttactacgct ggaggcggcg
     2101 atttcgcggt tgatacgcag gtgtttcatc ttcatgccgc gctcgaattt accggagcag
     2161 acgcgcaaga aggcgatacg gtcgcggtgt ttcgggtcca tattggcttg gattttaaag
     2221 ataaatccgg aaaatttcgg ctcgtccggc ccgaccatgc gcatggtcgc gtcgcgcggt
     2281 ttcggtgcgg gtgcccagtc aatcaatgaa ttgaggattt cctgaatgcc gaagttgtta
     2341 atcgcagagc cgaagaacac tggcgtgagt tcgccggcga gaaattcgtc gagattaaat
     2401 tcgttggaag ccgcctgcac caattcgatt tcgtcgcgca actgctggat ttccaacgga
     2461 aagcgttgtt ccaattcggg attgtttatg cctttgatga tgccgaactc gtgcggcagg
     2521 cgttcgccgc ccgcttcaaa gagatagatt tcgtcgttca ggatgtggta cacgcccttg
     2581 aagtttttgc ccataccgat cggccaggta acgggcgcgc agcggatttg caggatgtct
     2641 tccacttcgt ccaagagttc caaagaatcg cgcacttcgc ggtcgtattt gttcatgaag
     2701 gtaacaatcg gcgtatcgcg caggcggcag acgttcaaga gtttgatggt ttgcgcttcc
     2761 acgccttttg ccgcgtcgat gaccatcaag gcgctgtcca ctgcggttaa aacgcggtag
     2821 gtgtcttcgg agaagtcttg gtgtcccggc gtgtccaaga ggttgacggt gtggtctttg
     2881 tagtcgaact gcatcacgct tgatgccacg gaaatgccgc gctgcttctc gatgtccatc
     2941 cagtcggagg tggcgaattt gccggttttc ttacctttca ccgtgcctgc gctttgaatc
     3001 gcgcccgaaa acagcagcag tttttcggtc agcgtggttt tacccgcatc ggggtgggag
     3061 atgatggcaa acgtgcggcg gcggcgcact tggtcgagga tttcttggga catggttttc
     3121 tttgcaaaaa ggttcaggcc gcttttcaga cggcccggac agtgtttgag acggcgaaat
     3181 tgtacaaaaa aatgcctgat aattcaatgt tggaggcggt cagtgcgtgc tgccgtaaat
     3241 ctctttttcg tctttcagga cggcatcggc ggtttcccac gcaccgccgt tccatttttt
     3301 gtaaaagcag ctttcgcgcc cggtgtggca ggcgatgccg ccgttttggg cgatgagcat
     3361 cacaatggta tcgccgtcgc agtcgaggcg cagttcgcgg actttttgcg tgtgtcccga
     3421 ctcttcgccc ttcatccatt gtttttggcg cgaacggctg taatagtggg caaagccggt
     3481 ttcgacggtt ttttgcaggg cttcggcgtt catccacgcc accattaaaa cacgtttggt
     3541 ttcggcatct tgggcgatgg cgcaaaccaa acctttttcg tcaaatttga cggcttcaag
     3601 caggttttta tccatatttc ctttcagacg gcatagtcgg ggcggtcaga ggcgcacttc
     3661 gatgccggct tcgcgcatag tgcgtttggc ttcgcggatg gcgatttccc cgaaatggaa
     3721 aatgccggcg gcaagtacgg catcggcttt gccttcggtt atgccttcaa tcaggtgccg
     3781 gacattgccg accccgccgg aggcgatgac ggggatgtcg acggcttcgg caacggcgcg
     3841 ggtcagcggc aggttgaaac cctgtttcgt cccgtccctg tccataccgg tgagcaggat
     3901 ttcgcccgcg ccgcgttttt gcatttcgac cgcccattcc accgcatcca aaccggtcgg
     3961 attgcgcccg ccgtgggtaa agatttccca gcgtgtgttt tcggggttga cggctttggc
     4021 atcgacggcg gcgacgatgg cttgcgaacc gaaaaatccg gcggcttcgt taattaaatc
     4081 gggacgggta acggcggcgg tgttgatgct gactttgtcc gcgccggcat tgagcaggcg
     4141 gcggatgtcg gcaacggtgc gtacgccgcc gccgacggtc agcgggatga agacttgtcc
     4201 ggcaacctct tcgatgatgt gcaggatggt gtcgcggttg tcggatgagg cggtgatgtc
     4261 gaggaaggtc aattcgtccg cgccttcgcc gttgtagcgt ttggcggctt cgacggggtc
     4321 gcccgcgtcg cgcaaaccga tgaagttcac gcctttgacg acgcgcccgt cttttacgtc
     4381 gagacagggg atgatgcgtt ttgccagtgc cataatcgga tgcctttagt cgagggaatc
     4441 tgccagttgc tgcgcttggg caaaatcgat gctgccctcg taaatcgcgc ggccggtaat
     4501 cgcgcctgct acgccatgtt tttcggcggc acacagggcg gggatgtcgt ccaagccggt
     4561 cagtccgccg gaggcgatga cgggaatgcg gacggattgg gcgagtttga ccgtcgcgtc
     4621 gatgttcacg ccgctcatca taccgtcgcg cccgatgtcg gtgtagatga tgctgttgac
     4681 accgtcgtct tcaaagcgtt ttgccaaatc aattacatga tgcccggtta cggttgccca
     4741 gccgtcgatg gcggccatac cgtctttggc atccagtccg acgataatcc tgccggggaa
     4801 ggctttgcac gcttcgcgca ccaagtcggg gtttttgact gccgccgtgc cgataatcac
     4861 gtcgttcagt cccaaatcca aatattgtcc gatggttttc aaatcgcgca tgccgccgcc
     4921 gagctgcacg gggatgtctt tggcgacggc ggcgaggatg tctttgatgg cgggcaggtt
     4981 ttgcggaacg ccggcaaatg cgccgttcaa atctaccagg tgcaggcggc gcgcgccttg
     5041 ttcgaaccag tgcagcgcgg tgtcggcggg cgaatcggaa aagacggtcg cctcttccat
     5101 cagcccttgt ttcaagcgga cgcaacgtcc ttctttcaaa tcgatggcgg gtatcagcag
     5161 catagttttt ctccttgtgc ggggccgtgt cgggcttacc agtttaaaaa gtttttcaac
     5221 atcgtcagcc cggcatcgtg gcttttttcg gtgtgaaatt gcgtggcgaa tacgttgtct
     5281 ttgccgacga tgcaggcaaa cggggacggg tagtcgcttt cgcccaatat ggtttcggga
     5341 ttttcggggg cgaaatagta gctgtggacg aaataaaaac gcgtgttttg ggggatgcct
     5401 tgaaaaagcg ggtggttttg ggtttggcgc acggtgttcc agcccatatg cgggactttc
     5461 agacggcatc cctgcgggtc gcggaggtcg cgggcaaagc gtctgacttt gccgccgaac
     5521 cagcccaagc cgtcggtgtt tccttcttcg ctgtggtcga ataaaagttg cgcaccgacg
     5581 cagattccga aaaacggttt gttttttaag gcatctttga ctgcctcgtc caagccgccg
     5641 cgcgtcagtg ccgccataca gtcgggcatc gcgccctgcc cgggaaaaat gactttgtcg
     5701 gcgcgggaca cgcggtcggg gtcgccgctt aaaaagattt tggtattttt tccggcaagc
     5761 tgccccgccg tccggacgga tttcaatacg gaatgcaggt tgcccatacc gtaatcgata
     5821 atggcggttt gcatggcttc ctcctctttt ttgcaatatg gctgcgattt taacaaacaa
     5881 atgtgccggg ctgataaaaa tgccgtctga aaacgggaat ctgtcttcag acggcatagg
     5941 gttcaaaccc ggaaagccgt ttgtcagcct tccatttgtt ttgcctgaac ggcagtcagg
     6001 gcgatggtga acacgatgtc ttccaccagc gcgccgcgag acaggtcgtt gaccggtttg
     6061 cgcaggcctt gcagcagcgg gccgacgctc aagacgttgg cactgcgttg gacggcttta
     6121 taggtgcagt tgccggtgtt caggtcgggg aagaccaaaa cggttgcctg tcctgccacc
     6181 gggctgcccg gggctttgga tttgcctacg cccggcacgg ttgccgcgtc atattgcagc
     6241 gggccgtcga tggcgaggtc ggggcgtttt tcccgggcaa gtttggtggc ttcgatgacg
     6301 gtatcgacat cggggccgct gccggagttg acggtggagt aggaaatcat cgccactttc
     6361 gggtcgatgc cgaaggcttt tgcggaatcg gcagactgga tggcgatgtc ggcaagctgt
     6421 tgcgcggtcg ggttcggatt aaccgcgcag tcgccgaaca ccagaacctg atttggtagc
     6481 agcataaaga atacgctgga tacgaggctt gcgcccggtg cggttttaat cagttgcaaa
     6541 gcggggcgga tggtgttggc ggtggtgtga accgcaccgg ataccaaacc gtccacatca
     6601 ttttgcgcca tcatcatcgt accgagtacc acggtgtctt gcagttgctt gcgcgcgtct
     6661 tcgggtgtca ggcctttgga tttgcgcagt tcgcacatcg gctcgacgta ttgttcgacc
     6721 aatgaggcgg gatcgatgat ttccaaagag tcgggcaggc tgatgccgcg ttctttggca
     6781 acggcttcga cttcttcgcg tttggcaagc aggacgcagc gggcaatgcc tttttcgtgg
     6841 cagatggcgg cggcttggac ggtgcggggt tctgcgcctt caggcaggac gatgcgtttg
     6901 ccggcttggc gggcgaagtc gatcaggttg tagcggaatt gcgccggcga caggcgtttt
     6961 gcttcgcggc ctgccaatac ggatacgtct ttcagcgcgt cgctcgaacc gaagaaggtc
     7021 aggccggttt tctcggctgc cgcttcggca acggaggctg ccgcaccgtc cacgacaaaa
     7081 ccttccaata cgcccggcgc ggcggcgaag aactgtttgg caaggttcaa ttggtgtgcc
     7141 agcgcgtcgg catcggtatt gtcggaacgg acggcgaaga cggctgccgc gtcaagggac
     7201 aatgccagtt cgacgttttt gcctgcgaga tagattttgt cggcatcggg cgcgatgcct
     7261 tcgatgacga ggttggcggc atcgagtgcg gcaactttgc cgaccagtgc gtcgaaccag
     7321 tcgtcgcttt tgccttgcgc gagcagggtt tcggcggttg cgtcaacggc ttggaaaact
     7381 tgtgcgtcca gtgcttttgc aaaggcttgt gcggcggcgg aggtgttcag tccggcagat
     7441 acgggtacga cgagtacttt tgccataata tatcctttcg tatgctgcgg tgtgcggcat
     7501 atgtggttgg aaggggcggc atatgggcag aaacggctgc ctgcgtgccg tgcgtgccgt
     7561 gtttggcttg gggcgcgcag gttgaatata gcaaacaaat tctgtttcca acaagataaa
     7621 tatccgcagg cttgtggatg ctgccgcctt tcagagggta tttccgggga agaacagggc
     7681 gggaccgtcc aaatggagga cggcggaaat gccgtctgac agggtggggg cggaagggag
     7741 gttgagcgtg aggacggttt gtccggctct gaggctgatt tcggtatgcc gcgctttggg
     7801 cgtggttttg agaaccacgg cgtgaatgga ggcggcgggt gcggaatggg ggtgaaggct
     7861 gaactgttcc ggacggatga gcagtgtacc gcgcgtgcct gcgggtgcgc cgctttggac
     7921 gggcaggcgg cccaatctgc aatcggcggt gccgtcggcg ttgagcgcgg cggggaacac
     7981 gatgccttcg ccgataaaca gggcggcatc aaggtcggca ggttgtcggt acaattcgtg
     8041 agggcttgca gtttggagga tgcgcccctg tttcatcacg gcaatccggt cggcgtattg
     8101 cagggcttct tcgcggtcgt ggctgacaaa aacggcggat tttccgttgg cgcgcagggc
     8161 ggcaatcatg tcttcgcgaa tctggcggcg caactgttcg tccagcgcgc tgaagggttc
     8221 gtccaacaaa atcagttcgg ggtcgggggc gagggcgcgg gcgagggcga cgcgctgttg
     8281 ttgtccgccc gaaagttcgt gcggatagcg tccggcaagt tcggaaatgc cggtcaattc
     8341 caacatggct tcgatgcgct gtcgctcttg cgccgtcctg cctttgccgt tgccgagacc
     8401 gtaggcgatg ttgcggtaaa cggtcaggtg ggggaacagc acgccttcct gtacgaggta
     8461 acccaaacgg cgttcgcgga cgggaaggtt ggtatttttc gagaagatgg ttttgccgga
     8521 aagcgaaatt tcgccggaat cgggttgttc gaaaccggca aggcagcgta aaagggtggt
     8581 tttgccgcag ccggacgcgc cgatgataaa gagaatttcg cccgggtcga ggctgagcga
     8641 aatgtcgttt aaaaccgggg tgttttgaaa acttttggac aggtgtccga tgtgcagggc
     8701 ggcggtcata gcggtgcttc ctcaagctgt tatttgaagg cgtatttctt cagcaggaat
     8761 acgggtatgc cggaaaataa taccagcatc agcgcgtaag gggcggcggc ggcgtattgt
     8821 gcgtccgatg tgtattccca aacggcggtg gagagcgtgt ggacatcgtc ggcggtcagc
     8881 agcagggtgg cggtcaactc tttcatcagc ttgaggaaga cgagtgcgaa tgcggcggta
     8941 atgccgggca ggatggacgg cagtaccaac gtcctgaaaa taaagaagtg tccgcgcccc
     9001 aatgttgcgc cgacctgttc catccctttg gggagttgtt ccaaggaagt cctcagggtg
     9061 gtttgcgcca tcggcaggta aagcatgaaa taggcaagga tgacgacgat aaaggtttgg
     9121 taaacggcag gggtgtagtt gatgctgaaa taaaccaagg atagggcgat aaccaaaccg
     9181 gggacggcgt gcagtaaaaa cggcagcctg tctatccaaa cggttaaaaa attgcgatag
     9241 cgaaccgatg cccaaacaag gggcaaggca cataatatag tcaaaatcgc acctaaagcc
     9301 gatacgctta aggaacggat aaaggcatca aatacggata cgagcgcgaa tgtgccggaa
     9361 gtgccgacca tcatccaatg tatcaatacg ccaaagggga taataatgcc caaaatcaac
     9421 aagctgctta aaaaaacaat cgcgccgatc tgaccgggca gtttgagggt tttgacggga
     9481 taaggacggg caacgccttt gccgctgtgg taaatcttgg ctttgccgcg aaatatgctt
     9541 tctccaaata cgacgatgcc gcacaccgcc gtcaaaacag cggaaagcag ggcggcggta
     9601 ttgttgttgt aggacatttc gtattcttgg aaaatggcag tggtaaaagt ggggtagttc
     9661 aaaatggata ccgcgccaaa ttcgaccagc atatgcaggg cgatcagtaa cacgctgctg
     9721 ccgatggcgg gtttgagctg tgggaggatg gcggaaaaaa aggtttgcag gcggcttttg
     9781 cccaaggaca ggctgacttc ttcgtaagac aggctgatgc gtttgagtgc cgcctcgacg
     9841 ggcaggtagg cgagcggaaa cgaggacagg ctcataatca tcactgtccc ccaaaagcct
     9901 tcgacgcgga aggtcaggct gatccaggtg aaacagctga caaatgcggg gatgcacaaa
     9961 ggcagggtga ttgccgtctg aaaaaaggtt ttgccgaaga agcggtaacg ttggaacaaa
    10021 agggcgcagg caatgcccaa aacaatggaa atcagggtaa cgcccgccat catcgtcaag
    10081 gtgttggaga gcaaatccca catacgcggg cggaacaaca gttcgacggc gcggttgatg
    10141 ccgacctgcc acgaacgcat agcgacatat aaaaaaggca gggtaagcgg cagggcgatc
    10201 agtaggatga ggccggtaag ccaaatgggt atttttttag gagacatagt gtttttcatc
    10261 ggcaaaacgg gcggacagta taaatgtccg cccgtttgac aatccgaaaa cggcttattt
    10321 cataccggct tgctcaagca gccgggtggc gtgttctttt tcggaaacag tggtggcgga
    10381 cacttggggt gcttccaact tggcgatggg ttccaaattg aaggtggata ccacgtgcgg
    10441 attcaaagga tattcggcac ggacggcggt cagggcgcgc tgtccttcct tgccggcgag
    10501 gaaggcgacg aattttttcg cctcatcctt gttttgggag gattttaaca cggctgcgcc
    10561 ggaataggta acgagtgcgc cgggatctct gtggcggacg aaattcaggc gggtgtggac
    10621 attttgtacg cctttttcac gcgcgaaagc gtgccagtag tagttgttga tgagggcggc
    10681 atcgatttcg ccgttttcaa ccgcttgaag ggcgacggag tttttagcgt aaggcttgcc
    10741 gtattctttc aggcctttga gccatttcaa tgcggccgct tcgcctttca gtttgacgat
    10801 ggcgacaatc tgttccaaga acgcgccgga agtggggacg taaccgatgc ggtttttcca
    10861 tttcggcgtg gcgtaattca ggacggattt ttccaaatct ttttcagaca gtttgcgggt
    10921 gtcgtaaacg acgacgcgcg aacgtccgct cagtgccacc cagtcttttt tggcggcaac
    10981 cggcacgcct ttgccgcgtg tttcgttgat ggtggaggcg ggcaggggct ctaggaggtt
    11041 ggctgcggaa agggtggcga gtgccgggat ttgttcggaa tagaatacgt cggcggggct
    11101 tcggctgcct tcttctttga tttggccggc aagctggtcg cctttggcac tgttgagttt
    11161 gactttgatg ccggtagccc gggtaaaggc atctgcaacg gcttgtgccg cttctttgtg
    11221 ttggccgttg tacacggtaa tgtctgccag cgcgggggtg gcggcggtca gggctgcggc
    11281 aagcagtgcg tatcggatag atgttttcat atcgattttc tcctaaatga atgagggtgt
    11341 ataccttgtt aagacataac ggggtgtagt gtattccttc ttttttataa atgcaaataa
    11401 ttatttttta aatttgttat tatccgatcc ggttattgtt tgttctgact tgtatttttt
    11461 ccgtgcatcg cgcccgtaag gcggaagcgg cgggcaatgc gtggcggaat gtgggtaaag
    11521 gcggcatttt gatttgtcgg aatgctggag aacctctccc tttaaaacgc cgtctgaaca
    11581 aggttgccgg aatagtattg ccatcccggc agatacagtt tgtcgggatc ttgccaatat
    11641 tgttcatcca gactgttcgg cagcgaggcg gttttgttgt cgagatgttt tgttaatcca
    11701 ctatatatcc cacatttctt ttaggttttt accttccgat tggaggtggc ggatttcttt
    11761 tcccaatgcg tctgccgccc gatggatcag cattgcgctg tgtaccgcgc cgattttttt
    11821 cagaacggaa caagtctttt cggcggcggg aaaaccccag ttgcagacaa attgcagtat
    11881 gccgccgttg ccgatgtcgg cttccgcccg ccaaatcaaa accagctcct gttcccgtcc
    11941 gtccatgctt tcgagtttgc cgtcatgctg ttcaaaaagt ttgtccaccg cctgccgcat
    12001 catctgctcg aagcggtcgg cttgggtatc cgtatcggtc ataatattct tgccttttaa
    12061 aaatgccgtc tgaacatttc ttcagacggc atttgggggt taagctgaca tttcccgcca
    12121 gcgtttgact tggaggcgta cctgttcggg cgcggtgccg cccaagtggt tgcgcgcgtt
    12181 gaggctgcct tcgggtgtca gaacgccgta cacgtcgtct gaaatcagct tgctgaagcc
    12241 ttgcagggct tcgagcggca attcgctcaa atcaacgccg gcttcatcgg cgtggcgcac
    12301 ggcttgggca acgacttcgt gggcatcgcg gaagggcata ccttttttga ccagataatc
    12361 cgccaagtcg gtggcggtgg caaaaccctg catcacggcg gcgcgcatat tgccgggttt
    12421 gacggttacg ccgcgcatca tatcggcgta aatccgcagc gtgtcgatga gcgtgtcggc
    12481 ggtgtcaaac agcggttctt tgtcttcctg attgtctttg ttgtatgcca agggctgcga
    12541 tttcatcagg gtaatcaggc cgataaggtg tccgatgact cggccggatt taccgcgcac
    12601 gagttcgggt acgtcggggt ttttcttttg cggcatgatg gacgaacccg tacagaagcg
    12661 gtcggcgatg tcgataaagc cgaaacgcgg actcatccac aaaatcaatt cttcagacag
    12721 gcggctcaag tgaaccataa ccagcgaggc ggcggcggtg aactcaatgg caaaatcgcg
    12781 gtcggatacg gcatcgagcg agttctggca gatttgttcg aaacccaaca attcggcggt
    12841 gatttcgcgc tgaatcggat aggtcgtacc ggcaagggcg gcagcgccga gcggcatacg
    12901 gttgacgcgt ttgcgacagt cggccatgcg ttcgaaatcg cgtccgagca tttcgacgta
    12961 ggcgagcatg tggtgtccga agctgaccgg ctgggcgact tgcagatggg taaaaccggg
    13021 catgacggtt tcggcgtttt gttccgccaa atccaataat gccgtctgaa ggttttgaat
    13081 caggttttgg ataacggtaa tctggtcgcg cagccaaagg cggatgtcgg tggcgacttg
    13141 gtcgttgcgg ctgcggccgg tgtgcaggcg tttgcccgcg tcgccgattt tgtcggtcag
    13201 gcggcgttcg atgttcatgt ggacatcttc caaatcgagc ggccattcga ttttgccgct
    13261 gcggatttct tcgaggattt ccgacatgcc ccgacggatg tcggtcagat cgttttcact
    13321 cagcacaccc gaccgggtca gcatctgcgc gtgggcgagc gagccttgga tgtcccattc
    13381 ggcaaggcgt tggtcgaaac cgatggaggc ggtgtattgt ttgacgagtt cggaaacggg
    13441 ttcgttgaaa cgtccggacc aggttttgtc gtgcataagg attccttgat ggggttattc
    13501 ggtgcggtat ttttccaaaa ggcggcggaa gggttcgccg gtttcggggt gttttaaacc
    13561 gtaggcgacg gtggcttcga gatagccgag tttgctgccg cagtcgtagc gcgtgccttc
    13621 aaaggggtgc gccaagacaa attcgtgatc gagcagcttg gcgatgccgt ctgtaagctg
    13681 gatttcgttg cccgcgccgc gcggcaggcc ggttaaaagg tcgaaaatgc gcggggtgag
    13741 gatgtagcgt ccgacaacgg caaggttgga gggcgcgtct tcgggcttgg gtttttcgac
    13801 aatgccggta atgcgttgga actgtttgag ctgttcggtt tcgacgatgc cgtatgagcc
    13861 ggtttgcgac ggttcgacgg tttctacgcc caaaatgctg ttaccgctgc gcccgtacac
    13921 ttcgaccatt tgtttgagcg cgcctttggg cgcatcgatt aagtcgtcgg caaggatgac
    13981 ggcgaagggt tcgtcgccga tggcggcgcg ggcgcacaag acggcgtgtc ccaatcccag
    14041 tgcttccgcc tgacggatgt agaggcaggt aatgttcggc ggcaggatgt tgcggacgtg
    14101 ttccaacaat ttgtctttat ggcgcatttc caacttagtt tcgagttcgt atgccttgtc
    14161 gaaatggtct tcgatgctgc gcttgttgcg cccggtaaca aacaccattt ccgtgcagcc
    14221 ggcttccacg gcttcttcta cggcgtattg gatcagcggc ttgtcgacga tgggcagcat
    14281 ttctttcggg ttggccttgg tggcgggcag gaagcgggtt cccatccctg caacggggaa
    14341 gacggctttt tttatcggtt tcattctttt tcctttgtat tgttttgatg tttaaagggc
    14401 gagtttgcgg attaaatcgg cgagtgcctg cgcgcggtgg ctttcgcggt ttttgacctc
    14461 cgaatccaat tcggcggcgg ttttgccgtg ttcgggcaga taaaaatacg ggtcgtaacc
    14521 gaaaccgttt tgcccgagcg gcgtgtcgtg ccattgtccg tgccatatgc cctcggcgat
    14581 aatcgggcgc gggtcgtctt tatgacggac aaaaaccaat acgcagacat aagaacaact
    14641 tttgtcggcc ttgccggcaa gttcggcggc aagtttcagg ttgttggcgg tatcggattt
    14701 gggattgctg ccggcgtaac gtgcggaatg gatgcccggc gcgccgttta gggcggcggc
    14761 acagatgccg ctgtcgtcgg cgagtgcggg cagcccgctg tgtccggcgg cgtgccgggc
    14821 ttttgccagc gcgttttcga caaaggtggg atagggttcg gggcattcgg gtatgccgaa
    14881 tggggattgc ggcaatacgg tgatgccgta aggtttgaat aagttgccga attctttgag
    14941 cttgccggca ttgccgcttg ccaaaacgat tttttccggt ttttcagaca tagcggtttc
    15001 cctgtgatga agatggggcg gcgcgtaggg atttgtgccg caggtagagg gcgagtgcgc
    15061 cgatttgccc gaacagcgcg ccgaatgcga aaaggaagga cgcgacggcg aaggctttgc
    15121 tgccggcggt cagcagataa gcacccaaga ggacgggcag cataaatgcc agtgtgaaca
    15181 aggcaacaga caacagatag atttttcggc ggttcatggc gttcggtcgg aaacgggatg
    15241 ttcggattat agccgattag gacggcattc cctagaggct ggaaaaatgc cgtctgaagc
    15301 ggcgttcaga cggcatggcg ggcattatgc ctgtttgttc caacgttcga tggattcttt
    15361 gatgactttt ttcgcttctt ccgcaccgcc ccaggattcg actttggtcg tacccgcttt
    15421 tttcaggtct ttgtagtggt tgaagtggaa ctcgatttgt ttgatgagct gttgcggcaa
    15481 atcggacaaa gttttgtagg cgttgccgtt attgcggtcg tcggcaggta cgcagacgat
    15541 tttgtcgtcc acttcgccgt cgtcgacgaa tttcatcacg ccgataacgc gcgcttccaa
    15601 gaatacgccg gttgccagag gttgttcggt aacgagcaac acgtccaatt catcgccgtc
    15661 ttcgtccaaa gtttggggga tgaagccgta gttggtcggt ttggcgaaga tggcgggttc
    15721 gatgcggtcg agttggaatg cggcgagttt gcggttccac tcgattttgt ggttgctgcc
    15781 ggcggggatt tcgttgacga cgttgatgat gccgccgtcc acgtcgcccg gggtcaggat
    15841 ttggttgaag tctgccattt ggtttccttt atttgggaag gtttgaagtt tgaaagtata
    15901 gcacaaacgt ccggctggaa atgcgtccga tgccgtctga aagggaaacc gggcgcgtgt
    15961 taccgtttgc ccaaaaacct gcccagttcc aaaatcgcgc gcctgttgga cggggaaaac
    16021 actttttccg ccgcttcttc caagccgaac cagccgtagg agacgtgttc ctcgggttgc
    16081 aggacgacgg gcgtatcgcg cgggatttcg gcccggaaga cgtgttcgcg gttttcaaac
    16141 acgcctttgg ggtagcggtg ccgccagtgg tggtagattt cgtaaaccgt gctgtcgtgc
    16201 cggtcttgga gctgcccgtc ctccagcagg atgccggttt cttcccaaac ttcgcgcctt
    16261 gccgtttggg cgacggtttc gcccggttcg aggctgccgg ttaccgactg ccaaaatcct
    16321 ttcggatgcg tgcgttcgat gagcaggatg ccgccgtccc cgctataaag gacgaccagt
    16381 gcggaaacgg ggtatttgag cggttttgcc atcggcatct ttcggcgggc tgcggtaatg
    16441 aaggggcgga ttatagcaaa cgccgcacgt tatggcgttt atccttttcc gtatcctttt
    16501 ttctgcacgg atgggacgcg ccggtgtttg ccggtaaatt ttcctttgtg tcaaaaagat
    16561 aagggcggtt gtgattttaa tgcttgccaa agtgacgggc ggaaactata atccgcgact
    16621 taccgagtcg gagtgtggcg cagtctggta gcgcacttgc atggggtgca aggggtcgaa
    16681 ggttcgaatc ctttcactcc gaccaaaaat tccgaaagcc gctttcaaaa gcggcttttt
    16741 tgccgtccgt atgattatga tgtagagtac gcggcgacag acattcaaat gccgtccgaa
    16801 aaccgttcag acggcatctc tttatcttaa tagtggatta acaaaaatca ggacaaggcg
    16861 acgaagccgc agacagtaca aatagtacgg caaggcgagg caacgccgta ctggtttaaa
    16921 tttaatccac tatagtttca ttccgtacca tcttaaggaa catcaaattg ggcatttccc
    16981 gcaaaatatc ccttattctg tccatactgg cagtgtgcct gccgatgcat gcacacgcct
    17041 cagatttggc aaacgatccc tttatccggc aggttctcga ccgtcagcat ttcgaacccg
    17101 acgggaaata ccacctattc ggcagcaggg gggagcttgc cgagcgcagc ggccatatcg
    17161 gattgggaaa catacaaagc catcagttgg gcaacctgat gattcaacag gcggccatta
    17221 aaggaaatat cggctacatt gtccgctttt ccgatcacgg gcacgaagtc cattccccct
    17281 tcgacaacca tgcctcacat tccgattctg acgaagccgg tagtcccgtt gacggattca
    17341 gcctttaccg catccattgg gacggatacg aacaccatcc cgccgacggc tatgacgggc
    17401 cacagggcgg cggctatccc gctcccaaag gcgcgaggga tatatacagc tacgacataa
    17461 aaggcgttgc ccaaaatatc cgcctcaacc tgaccgacaa ccgcagcacc ggacaacggc
    17521 ttgccgaccg tttccacaat gccggcgcta tgctgacgca aggagtaggc gacggattca
    17581 aacgcgccac ccgatacagc cccgagctgg acagatcggg caatgccgcc gaagctttca
    17641 acggcactgc agatatcgtc aaaaacatca tcggcgcggc aggagaaatt gtcggcgcag
    17701 gcgatgccgt gcagggtata agcgaaggct caaacattgc tgtcatgcac ggcttgggtc
    17761 tgctttccac cgaaaacaag atggcgcgca tcaacgattt ggcagatatg gcgcaactca
    17821 aagactatgc cgcagcagcc atccgcgatt gggcagtcca aaatcccaat gccgcacaag
    17881 gcatagaagc cgtcagcaat atctttacgg cagtcatccc cgtcaaaggg attggagctg
    17941 ttcggggaaa atacggcttg ggcggcatca cggcacatcc tgtcaagcgg tcgcagatgg
    18001 gcgcgatcgc attgccgaaa gggaaatccg ccgtcagcga caattttgcc gatgcggcat
    18061 acgccaaata cccgtcccct taccattccc gaaatatccg ttcaaacttg gagcagcgtt
    18121 acggcaaaga aaacatcacc tcctcaaccg tgccgccgtc aaacggaaag aatgtcaaac
    18181 tggcagacca acgccacccg aagacaggcg taccgtttga cggtaaaggg tttccgaatt
    18241 ttgagaagca cgtgaaatat gatacgaagc tcgatattca agaattatcg gggggcggta
    18301 tacctaaggc taagcctgtg tttgatgcga aaccgagatg ggaggttgat aggaagctta
    18361 ataaattgac aactcgtgag caggtggaga aaaatgttca ggaaacgaga agaaggagtc
    18421 agagtagtca gtttaaagcc catgcgcaac gagaatggga aaataaaaca gggttagatt
    18481 ttaatcattt tataggtggt gatatcaata agaaaggcac agtaacagga gggcatagtc
    18541 taacccgtgg tgatgtacgg gtgatacaac aaacctcggc acctgataaa catggggttt
    18601 atcaagcgac agtggaaatt aaaaagcctg atggaagttg ggaggtgaaa acgaaaaaag
    18661 gtgggaaagt gatgaccaag cacaccatgt tcccaaaaga ttgggatgag gctagaatta
    18721 gggctgaagt tacttcggct tgggaaagta gaataatgct taaggataat aaatggcagg
    18781 gtacaagtaa atcgggtatt aaaatagaag gatttaccga acctaataga acagcatatc
    18841 ccatttatga atagtaatat ttatgaaaaa ttaggagatt aatgatgaaa agaattaagt
    18901 gcttttgtga taaatttcca tcaggagata catttagaat gtgtatcatt ctggatgact
    18961 atgataatag ggttgattat tatgtaggaa tatatgatta cattacgtct accttaatga
    19021 gcgatattta ctatcgatcc acgattgatg agcatttcaa gattatagaa ttaatagaaa
    19081 ataatccaaa tgaaatttat gatgatggcg gtggtcaaca attttgccta gaatttcatc
    19141 atgataaggt cattttttat cacaatgaat ttgatgaaga agatggttat ccagtattaa
    19201 gctgttcgct gcatactttt aaaactgctt taattgcttg gaatgctttt ttgcaattgc
    19261 ctaaaagtat tcattcggtg gtggagactg tgattgagga ataagcataa ttagcttaat
    19321 gaatagaatc agcgatatag attggactgc aaatccacgc ttatacgctg tgccatgatt
    19381 aacaggaaga gagcaacttg gatgggctag tagggatggt aagcatttaa atatatcaat
    19441 tgatggaaag attacacaca aatgactaat ttaaaattag atttctactc tgaagttatt
    19501 ataaaagatt cttgccctaa tgatttgtta gaaaatggtg agactattaa aggaaaaaaa
    19561 ggagtggtat taggtataag cgaagaagat ggtataatct atggctatac tattttactt
    19621 tttgatataa aatattgtat atatatagat aagaaatata ttataccgac tggaaaaaaa
    19681 ttctctcgcg atgattttta ttagctaatt taagtaattt aagcaagttt tttagatact
    19741 gaaactggta attatgttta tgtaagcata aggagttcag attgaaggat tctacagggc
    19801 aaatgaggct ggcaaccaag gatttggcgg aagccattaa acgaggagaa gtacgtagtt
    19861 ctgcttttac aacaaagcaa ctaaaggcaa tcgaaaaagg caaagacaaa atccctagct
    19921 acacttggca tcatcatcaa gatacaggga gaatgcagct tgtgcctgaa tgggaacatt
    19981 ctaaaaccgg tcacatagga gggacggcaa tggggaaggg taaataatta tgtggaaaat
    20041 ttataaagaa aatagcaccg acttaaattt tgccataggc agtatatatt gccaagcaat
    20101 caatcttacc gaatttaaaa tgtgggtcga aaaaatcata agggaagtgg atttagatga
    20161 aattccaaat tatttttttg atttgataga ttttcaatca ctatacgatc tgattgatat
    20221 tataggattt gttcccgaaa ataacttatc aaaaaatcag gataatgcat taaccggtat
    20281 tgctttttta agggggatag atgtctacga tcctcccatt tcaaaagaaa aagcattaaa
    20341 agccttagaa aaacatcctg aaatttatca gaagtttcag catttctttc cgtttgtaga
    20401 gctgcctccg ctttaaacag tcaaaatgcc gtctgaaacg atattcggct ttcagacggc
    20461 atttttgata taaagcggat aaccaaagaa atgtttgacg gcaaaggaac atctgaaata
    20521 ccaaattaca cttggcatca tcatcaagat acaggaagga tgcaattgat tcgtgaagac
    20581 tcgcatcatg ataccgacca tatcggttgg agagcgatga gtaaaggaaa gtaactatgt
    20641 ggaaaatcat aaaagaggat agtgatgatt taggatttgc aattaaatgc ttattctctc
    20701 aatctattga tttaaatgaa ttcaagttat ggattgaaca agtaatacgc gatatgccca
    20761 tcgaggacat ccctttttat atttttgact tggcggattt caatggggga attggagaca
    20821 ttgacaatat tgtaggtttt gtttcgagtt acagcctatc aaaatcgaaa aaaaatgcct
    20881 tgacaggcat tgccttctta agggggatag atgtctatag accctcccgt ttcaaaagaa
    20941 aaagcattaa aagccttaga gaaatatcct gaaatttatc ataaatttaa acggttcttt
    21001 ccttttgtag agcttccgct tctttaaagg acaatatgcc gtctgaaaag ttttcagacg
    21061 gcatttttat ttcttccagt aggcgggggt gaagaggatg aagacggtga agatttccag
    21121 cctgcccaag agcattgcgg taacgcagat ccatttctgc atcacgtcca aaccggcgta
    21181 attgccggcg ggcccgactt cgcccagtcc ggggccggcg ttggtaatgc aggcgatgac
    21241 ggcggtaaag gcggtggtaa attccatacc gctcgccatc agcaggaagc tgaagaggac
    21301 gacggtcata aagtagatga agatgaagga cataacggtc agcgcgaggc ggtcgggtat
    21361 ggctttgccg ctgattttga cggtgcggac ggctttgggg tgcagcagca ccatcatttc
    21421 gcgcaggctg aatttgaaca ggacgagggc gcgtatggtt ttgatgccgc cgccggtcga
    21481 gccggagctg gcgaggatgt tggcgaggaa aaacatccac agggaaatca ggagcggcca
    21541 ttgtgcgaag tcggtgttgg acagcccgtt tgccaatccg atggagacga agttgaaggc
    21601 ggtgtagcgc agggattcgc cgaagccggc gtaatggccg gtgtgccaca ggtacagggc
    21661 ggcggcaagg atgctgccgg agagcagcag cagcatcgtc cggcattctt cgtctttcca
    21721 ataggttttg aggctgcggc tgttgagggc ggcgaaatgg ctggcaaaat tgatgccgcc
    21781 gaaaatagtg aaaacgatga tgactgcttc gatgaggggg gagttgtaat aaccgatgct
    21841 ggcatcgtgg gtggaaaacc cgcccagcga gagggttgcc agcgcgtgac agacggcatc
    21901 gaaccaaccc atcccggcaa aatgcaggca ggctgccgcg aggatggtga tcagggtgta
    21961 gccgaaccag agttttttcg ccacttggga aatgcgcggc gacattttgc tttctttgtc
    22021 aatgccgggg atttcggctt tgaataactg cgtgccgcct acgccgagca taggcaggat
    22081 ggcgacggca aggacgatga tgcccatccc gcccagccag ttgagcatat gccgccaaaa
    22141 gttgacggag ggggcgagcc cgtcgacgtg ggggatgacg gtcgcgccgg tggtggtcag
    22201 tcccgacatc gattcaaaaa atgcgtcggt aaagcccata ttcgggaaat acaggtacat
    22261 cggcatcgca gccatagcgg caaacgccag ccacaacatc aggacgaggg tgaagccgtc
    22321 gcgcgggcgc agttcgcgcc tgaaccggag ggtggcgagc cggacgatgc acgagccgga
    22381 aagggtaacg gtcgcggtgg tggcgaatgc ggtgtacgcg ccgtccgaaa aggcgtagga
    22441 aagggcggcg ggtatcagca ggataaagga aaacagcata cccagtcggg agaggacgtg
    22501 ggcgatgggc aggattttgt gcatagtggg gcggtccgtt attttgcgaa gcttttccag
    22561 tctatgccgc cggcggcttg gacttgggta atttcttccg tttcgaggtt gacggcggtc
    22621 agctgcccgc cccacagcgc gccggtgtcc agcgagatga cgttgtcggc attcgtgtag
    22681 cccagcgagg accagtgtcc gaagatgatg gtgtggtcga ggttttgccg atcgggggct
    22741 ttgaaccacg ggcgcaggta aggcggcatt tttttcactg tggatttgta gtcgaaatcc
    22801 agttcgtttt taaaggtcag ggcgcgcatc cgcgtgaagg cgttgacgat gaagcgcagg
    22861 cgggcatagc cttccaaacc ttcgtcccat gcggccggtt tgttgccgta cattttggag
    22921 aagaatttga cgtatttttt gccgcgcagt tcggcttcgg cttctccggc gagcgattcg
    22981 gctttggcta tgcgccattg cggcaggatg ccggcgtgta tcatcacgcg gccgccctcg
    23041 cgtatcaaaa gcggttgcgc gcgcagccag tcgagcattt ttccgccgtc ggggtgtttg
    23101 agtatgggtt cgattgtgtc gctgcgtttg agcgcgcctt cgccgcagcc gacggcgagc
    23161 aggtacaggt cgtggttgcc gaggacgatt tgcacgctgt tttcgtgccg gatgcagaat
    23221 tgcagcgttt cgagggattt cgggccgcgg ttgacgatgt cgcccgtcag ccagagggtg
    23281 tccgtgccgt ggttgaagcc gattttgccg agcagcgcgg tcagttcgtc gaaacagcct
    23341 tggatgtcgc cgattgcgta atgtgccatt gcgggtgttg tgaagtggga aagtgttgcg
    23401 gttcggacgg cacggttgga aatcttatgc cgtccgaacg tggaattatg cgttcaaaac
    23461 gaggacggct tccgcttcga cctgcacgcc tttgggcagc gaggcaacgc cgacggcggc
    23521 gcgggcgggg aacggctcgg tgataaattc cgccatcact tcgttgaaga cggcaaaatt
    23581 gcccaagtcg gtcaggtagg cgttgagttt gacgatgtcg tccagcgaac cgcctgccgc
    23641 ttcggcgacg gtctgcaggt tttggaacac ttggcgcgct tcggtgtgga aatcgccgtt
    23701 gccgaccaca gtcatcgtgg cgggatcgag cggaatctga ccgctcatgt aaacgatgcc
    23761 gccggcgcgc acggcctggc tgtatgcgcc gatggcggcg ggggctttgt cggtatggat
    23821 gacggttttg gacatttcgg attcctcaaa aaatagggcg gcagaagccg cagcgttcgg
    23881 gattatcgta caaaaccgcc ggcttgtgta gttttaggcg gtatatgtgc cggaaacaaa
    23941 aaaccgccga aggctcggcg gtttgcggaa taacgcgtat atcagaattt gacgcgcacg
    24001 ccggcggaca gttcgccgga acggacgttt ttgacattgt tgactttgcc gatgtagttg
    24061 tagcggtagc cggcatccag atcgacattg ggtgtaacgg catagcttac gcccgccaat
    24121 acgccgaggc cggcggaggt tttgctgaag ctgtcgctgc cgcccaagtg ggcggaagcg
    24181 cggttgaggc tcaagcgcgc gccgaaatac ggtttgacgg gcgattgggt gtcgaagtcg
    24241 taaatgacgg acgcgccgat gctgtaaagt ttgaaatcgg tggatggggc ttgtttatag
    24301 tttttgtagc gcgtgtaatc gacggcgaag cggaggtcgt tgatgcggta gcctgcggag
    24361 atgcgcgggc tgaagccttt ggcagaacct aaagagcttg aggctttggc gtgtgcggca
    24421 tcggcttgga cgtaaaagcc ggatgcgcct tccgccagtg cggcggccgg gagtgcgagg
    24481 gcaatcagtg cggcaagtgc ttttttcata ttttggttcc tttatggtca gggagaaaaa
    24541 ttgttaataa tccgttaaag aatcctgccg tattatactg aaattttctt tttgcatcgt
    24601 aatattttca atgcctcaag atacgcggcg gcatccggct gctttgccga cggcaaagcc
    24661 gttaacccgc gcgttgccct taaacggcgg catcacgcgg cggatgggtg aaacttgcaa
    24721 acggtttgga aaaaaccgca aaggcggggc ggcggcattt gtcagattgt tgcaggcgca
    24781 ggcgtacggt tttttgtgtg cggcgttacc ttaggcgtcg gacatttccg gcggcggctg
    24841 tgccgtctga aacgcccggc gggggatgcg gctgcgtttc ccatcgatag gcatatttgc
    24901 cggccgcgtt cggggcgggt tttacccggg cggccgccga tttgtttgcg cttataaaaa
    24961 acactgcaac aaatcgttta aaaacaagcg tcctttttcg gtcgggcgga atacggtcgg
    25021 gtcggtttcc agcaggcctt tttgccttgc cgtttcgatt tgcaccatga ttttggcggc
    25081 gggtacgccc gtgcgctcct gcaacatcgc ggcgggtacg ccgtcggtca ggcgcagggc
    25141 gttcatcatg aattcgaacg gcaaatcttc ggcggcgacg gttttgcgtt cgacggcttc
    25201 gcccggctga ctttgcatta aggcgaggta gtcgttgggg tggcggcggc ggacggtgcg
    25261 ctcgatgcgg tcgggatagg agattttgcc gtgcgcgccc gcgcctatgc ccaaataatc
    25321 gccgaactgc cagtagttca aattgtggcg gcactgcatg gtgggtttcg caaaagccga
    25381 tgtttcgtag tggataaaac ccgcgccttc cagtgtgccg tgtaccgcgt cttcgatgtc
    25441 gagcgccgct tcgtcttgcg gcaaaccttt cggcggcgta tgaccgaacg gcgtgttcgg
    25501 ctccatcgtc agatgatatg cgctgatgtg ggttgcgtct gtggcgatgg cggtttgcac
    25561 gtcgtccaat gccgtctgaa cggtttggtt cggcagggag tacatcaagt cgatattgac
    25621 tttatcaaat aattttaagg cggtatcgat agcggttaag gcttctttgc cattgtggac
    25681 gcgccccagc cttgagagca tatcgtcgtt gaaactctgt acgccgatag aaagccgcgt
    25741 gatgcctgcg tctttaaatc cttgaaactt ctcgatttca aatgtacccg gattggcttc
    25801 caacgtaatt tccgcttcgg gctgcaagcg caacagcgaa cgcacgccgc ttaacaaacg
    25861 gtcaatcgat tccgcctgaa acaggctggg cgtaccgccg ccgaaaaata tcgtttccac
    25921 cggcctgccc caaatattgg gcaattcaag ctgcaagtcg gtcagcagcg cgtcgatata
    25981 ggcggcttcg ggcaatccgt ttttcaggct gtgggaattg aagtcgcaat acgggcattt
    26041 tttgatgcac cacgggatgt ggatatagag cgacaggggc ggcagggcgg tgagtcgggt
    26101 gcggtttgga aaggaaatgg tgtgcatggt gcggttcgga aaagtggacg atgccgtctg
    26161 aaggcggttc ggacggcaca ggttcagccg agcagggtaa gcagttcggc ttcgctgagg
    26221 acggaaacgc ccaaggcatt ggctttttcc agcttgctgc ccgcggcttc tccggcgacg
    26281 acgtagtcgg tttttttgga cacgctgccg gacactttgc cgcctgcggc ttcgattagg
    26341 gcttgggctt ggtcgcgttt gaaggtgggc agggtgccgg ttaacacaaa ggttttgcct
    26401 gccacggctt tattgatgcc gtctgaacct tgcgccgcct cgtcttcaga cggcatttgc
    26461 gcgaagaagg tttttaggtt ttcgagcagg gcggtgtttt gcgctttgct gcgccacgcc
    26521 tgccagtcgg cagggagggc tttgtcgttt tgcagtcctt ctatactttg tccggcgagt
    26581 tcccataagg cttgggcttt gttttcgctg attttgaaac cgggcaggcg ggtaatccag
    26641 cgttgcggcc cggcatattg tgcggcaggc aggctgacgg cttgggcttg cggggcaacg
    26701 cctgcggcga gcagctcgtc tatcatcgcc tgctgttcgg cttgggcgaa gaagtgggca
    26761 atggaacgcg ccactaccgt gccgatgtcg ggcaggcagg cgaggacggg ttcgggggcg
    26821 cggcggacgc gttccaatgt gccgaatgcc tgcgccagcg ttttggcggt gcgttcgccg
    26881 acgtggcgga tgccgagcgc gaacaggaag cgggcaagtt cgggcgtttt gccggattct
    26941 atgcctgcga ggatgttttg cgcccacttg gtcggttgtt ttttgccgtt ttgggtattg
    27001 tatttagaca ggtcgcctga aaccgtttct gcgtcgccgt tttcgttttc agatgaccct
    27061 ttatccgccg tttccttcat tttttgcagg gtcggaatat cgaggcggta gaggtcggcg
    27121 aaatggcgga cgaggtcttg ggcgaccagc tgttcgattt gtttttcacc caagccgtcg
    27181 atgtccatcg ctttgcgcga ggcgaagtgg attaagcctt gcgcgcgttg tgcctgacaa
    27241 agcataccgc cgctgcatcg ggcgacggct tcgccttctt cgcgttcgat ttcgctgcgg
    27301 cagatggggc agcgggccgg caggcggtag ggcttatgga gcggaacgga ttcggtttgc
    27361 ttggcggacg gtgtttcggc aaacaggtcg tcctgctgat gcccgatgcc gtctgaaacg
    27421 gcaacggcgg tttcctgcat cgggcggcgt tcgaaaatca cgcgcacgac ttcgggaatc
    27481 acgtctccgg cgcggcgcac gacgacggta tcgccgacgc gcacgtcttt gcgcgatact
    27541 tcgtcctgat tgtgcagggt ggcgttggta acggttacgc cgccgacgaa tacgggttgc
    27601 aggcgggcaa ccggcgttac cgcgcccgtc ctgccgattt gcacgtcaat cgcttcgaca
    27661 atggtcaggg cttcttcggc agggaatttg tgggcaaccg cccaacgcgg cgcgcgggag
    27721 atgaagccga gttcgcgctg ttgcgccaag ctgttgactt tgaccaccat gccgtcgatt
    27781 tcgtagggca gttcggggcg tttttgctgc atgtgttcgt aaaacgccaa tacttcgccg
    27841 atatttttga aacagccgaa attgccattg ggcagactga agccgagttc ttggaaatag
    27901 gcgagttctt ggatgtgttc ttcagcttcg aaaccgcctt gctgacgggc aatcgaatag
    27961 gggaaaaagt gcagtttgcg ttgcgcggtg atgcgcgaat cgagttggcg caggctgccg
    28021 gcggcggcgt tgcgcggatt ggcaaagggt ttttgcccgt tttcggcttg gcgtttattg
    28081 agcccggcaa agtcggcttt gagcatcagc acttcgccgc gtacctcgat gagttcgggc
    28141 acgttttcgc cgtgcagccg cagggggatg ttggatacgg ttttgacgtt tcgggtaacg
    28201 tcttcgcccg tcgtgccgtc gccgcgcgtt gccgcctgca ccaatacgcc gtcgcggtag
    28261 agcaggctga tggcgaggcc gtcgaatttg ggttcgataa cgtattcggg attgccgccg
    28321 tccaagccgt cgcgcacgcg ttggtcgaag gcgtacattt cggcatggtc gaacacgccg
    28381 ttttcatctt gcggggaaaa ggcgttggtc agcgacagca tcggcacttc gtggcgcact
    28441 tcggcaaagc ctgccaaagg ctcgccgccg acgcgctggg tcgggctgtc gggcagcttg
    28501 agctcgggat ggtttcgttc caacgcttcg agttcgcgga acaggcggtc gtattcggca
    28561 tcgggtatgc tgggcgcgtc gagggtgtag tattcgtagg cgtagcggtt gaggaggtcg
    28621 gtgagttcgt gtatgcgttg tgcggtcggg ttcatggatt cgtacaggtt cagacggcat
    28681 ggacaaatgc cgtctgaacg gtaaaacgtg ttggaaatgg tcggatttta cctgaaaaca
    28741 aaaggcggat gcaccgttgc cgatgtatcc gcctttgtgt ttgcggagtt atgaaaacag
    28801 gcgcagggcg gttttgccgc ccggttcgat accgactttg agcatctcgg actgacgcgc
    28861 caatacataa gtgcgtacgt ctttgagcca ttgggtcgaa acttcttcca ttttgtcgtt
    28921 gaccagattc aggttcaact gaccggacag gcgtaccgcc aaatccataa acaaatcgtc
    28981 gaaggttttt tcgcctgccg gagagtgcgg gatgtcgagc agcatactga agcctttgta
    29041 ggactggttg tccaaaaggg cattggtaaa cggctcgttg ttgagcgagc agatggagaa
    29101 catggtcgag cccgacgtgt cggtatagtg gaacgcgccg tcgtcttcca aaacgaaacc
    29161 cacgcccgtt acggcggaac gcagttctac gccgctgatg ctggtcggcg aaaccaaatg
    29221 gatggcgatg gtctggtcga cgcgcgcgca gaatgcgtcc agtgcggaag ccacttcgat
    29281 aaaggcggca aggtcggtgt gcagcgtctg accgcccatg ctttgtgcga atgcgtccgc
    29341 ctggcggttg aatgcggaga gttcttcctg cgaggcaagt ccgttgcggc tgactgcctg
    29401 gatacccacg ataaatgcct gatagcggat gcccgggatg ggttcggcaa tctggaaatg
    29461 gtcgtccatg gtgcagccga caatctggta gcggcagcgg ttggaaaggc gcggcagtgc
    29521 gtgcagttct ttggcttcgg tcagcgcgat ataggagatg aagtcgaagc gcacgtcaaa
    29581 ccagggcagc tcgaccttcg acagctcttt gagcgtaatc agcggttttg ccggcgtttg
    29641 cggaacggga acgggttttg ccggcgcgtc ggcaggtttc ggtgcggaat gtccggtttg
    29701 gggttcggag acggtgtggg cggagttgcc gataatgccg ctttcttcca aggcggtttc
    29761 gatttcggtt ttgaacgggg aggcttttgc ctgtttctgc ttggcgatgt agacggcatc
    29821 ctgttcttgc aggttgcgca tggcggagtc ttggggtttg gccggttttt tgaccgccgg
    29881 ttggggtttc ggcatcatga ctggcccgcc ggacggtttg ccgtcgcgga catggctggt
    29941 tttgctgttg agcagggcat ctttgtcgga gtgtccgaac tggtcgcgca cttttttgcg
    30001 gtattggttt tcctgataca tattgtaggc gacaacggcg aggacggcgg cgaggaacag
    30061 tacgatgtaa atcatggcaa tcactttaaa tttcgggatg caggatgcgc aaagtgcggg
    30121 tactgcggtt aaatcgggct tgcgtttccg gcagtctgac ggaacggccg gttataacgt
    30181 ttgaattata acgaaaattg cagggtctga cagcagtgtg tcgaaataag cggaaatttt
    30241 ccgaaatgcc gtctgaaatc tgtggttttc agacggcatt tctgtccacg ggaaaccctt
    30301 tttcccgtat ccgccgccag tcgaaaaaat ggccggggtc ggttttgcgg ccgggtgcga
    30361 tgtcttggtg tcccgttacc gccgtgatgg ggtagcggcg gcagagggct gccaacagcg
    30421 tttcgagcga acggtattgc gcttcggcaa acggttcgaa atcgcagcct tccagttcga
    30481 tgccgatgga aaacgcgttg cacttctccc gcccccgaaa cgaggatgcg ccggcgtggt
    30541 acgccatgtc gccgcatgat acgaactgca ccgtttcgcc gtcgcgtgca atcaggaaat
    30601 ggctggacac gtgcaaagtg tgtatcaggc tgaagaacgg atgtccgtcg gggtcgagcc
    30661 ggttggcaaa cagcttttcc accgcatccg tgccgtattc gaacggcggc agcgaaatgt
    30721 tgtgcagcac gatcagggaa accgtttctc ccgtttccct cgggctgaaa ttgggcgacg
    30781 gggtatggcg tatgctttga agccagccgt tttgccagtg tgctccggcg tgattgtcca
    30841 tgatgttctt cctgtccggc gggcaatttg ggttatactg tcgcccgaat tttaagacgt
    30901 attccgaatg ctgggaatcc taccatgttg aaaaaaatgt tgaaatggac tgccgttttt
    30961 ttgaccgtat cggcagccgt tttcgccgcg ctgctttttg tccctaaaga caacggcagg
    31021 gcataccgaa tcaagattgc caaaaatcag ggtatttcgt cggtcggcag gaaacttgcc
    31081 gaagaccgca tcgtgttcag caggcatgtt ttgacggcgg cggcctacgt tttgggtgtg
    31141 cacaacaggc tgcatacggg gacgtacaga ttgccttcgg aagtgtctgc ttgggatatc
    31201 ttgcagaaaa tgcgcggcgg caggccggat tcggttaccg tgcagattat cgaaggttcg
    31261 cgtttttcgc atatgaggaa agtcatcgac gcaacgcccg acatcggaca cgacaccaaa
    31321 ggctggagca atgaaaaact gatggcggaa gttgcgcccg atgccttcag cggcaatcct
    31381 gaagggcagt tttttcccga cagctacgaa atcgatgcgg gcggcagcga tttgcagatt
    31441 taccaaaccg cctacaaggc gatgcaacgc cgcctgaacg aggcatgggc aggcaggcag
    31501 gacgggctgc cttataaaaa cccttatgaa atgctgatta tggcgagcct gatcgaaaag
    31561 gaaacggggc atgaggccga ccgcgaccat gtcgcttccg tcttcgtcaa ccgcctgaaa
    31621 atcggtatgc gcctgcaaac cgacccgtcc gtgatttacg gcatgggtgc ggcatacaag
    31681 ggcaaaatcc gtaaagccga cctgcgccgc gacacgccgt acaacaccta tacgcgcggc
    31741 ggcctgccgc caaccccgat tgcgctgccc ggcaaggcgg cactcgatgc cgccgcccac
    31801 ccgtccggcg aaaaatacct gtatttcgtg tccaaaatgg acggcacggg cttgagccag
    31861 ttcagccatg atttgaccga acacaacgcc gccgtccgca aatatatttt gaaaaaataa
    31921 accatgccgt ctgaaaagtt tgtgttttca gacggcatac ccttaccgga actgcaagca
    31981 tgaaaccgca attcatcact ttggacggca tagacggtgc cggaaaatcc accaaccttg
    32041 ccgtcatcaa ggcatggttt gaacggaggg ggctgcccgt gctgttcacg cgcgagccgg
    32101 gcggaacgcc ggtcggtgag gccttgcgcg aaatcctgct caaccctgaa accaaagccg
    32161 gtttgcgtgc ggaaacactg atgatgttcg ccgcgcgtat gcagcacatc gaggaagtca
    32221 tcctgcccgc gctttcagac ggcatccatg tcgtgtccga ccgttttacc gatgcgacct
    32281 tcgcctatca gggcggcggg cgggggatgc cgtctgaaga cattgaaatt ttggaacatt
    32341 gggtgcaggg cggtttgcgc cccgatttga ccctgctgtt ggatgtgccg ctggaagtat
    32401 cgatggcgcg tatcggacag gcgcgcgaga aagaccggtt cgagcaggag caggcggatt
    32461 tctttatgcg tgtgcgcggc gtttatctcg accgagccgc cgcctgtccc gaacggtacg
    32521 ccgttatcga cagtaaccgc agcttggatg aagtcagaaa cagcatagaa aaagtgttgg
    32581 acggacattt cggctgttga tgcggcaaat attgaaacaa gcgcatccgc ccgcgccgaa
    32641 aaccaaacgg cagtgccgca ggtgaaaatg gcggtatgcg ccaaactttc ggcatgatag
    32701 aattacgctc ggttacaagg caggatgcgt cggcaatatt aacgaaccgc ccgtaacatg
    32761 atgacccgaa agcgtttcgg acagttcgat tcaaatcttt ttctcgcaac aggattgaca
    32821 catggaaaac tcattgaaag aagccgccct caagttccac gaattacccg ttccgggcaa
    32881 aatttccgtt accccgacca aatctctggc gaccgacaaa gatttggcgt tggcgtactc
    32941 tccgggcgta gccgccccct gtatggaaat ccatgccgat ccgcaaaatg cctacaaata
    33001 caccggcaaa ggcaacttgg tcgccgtcat ttccaacggt acggccgttt tgggcttggg
    33061 cgacatcggc gcgctggcgg gcaaacccgt gatggaaggc aaaggcgtat tgttcaaaaa
    33121 attcgccggt gtggacgtgt tcgacatcga aatcgacgaa aaagacccgc aaaaactggt
    33181 ggacatcatc gccgctttgg agccgacctt cggcggcatc aacctcgaag acatcaaagc
    33241 acccgagtgt ttctacatcg aacgcgaatt acgcaaacgc tgcaaaatcc ccgtatttca
    33301 tgatgaccag cacggcacgg ccatcattac cgccgccgcc gtattgaacg ccctgcgtta
    33361 taccggccgt aaaatcgaag aagcgacttt ggtgtgctcc ggcgcaggtg ccgccgcgat
    33421 tgcctgcctg aaccaactgc tggatttggg cttgaaacgt gaaaacgtaa ccgtttgcga
    33481 ctccaaaggc gtgatttacc aaacccgcga agacaaagac cgcatggacg aatccaaaaa
    33541 attctacgcc gttgaagaca acggccggcg cgtacttgcc gatgcggtta aaggcaaaga
    33601 catcttcttg ggcctctccg gcgcaaacct gctgacgcct gaaatgttga acacgatgaa
    33661 cgaaaaaccc atcgtgttcg ccatggccaa cccgaatccg gaaatcctgc cgccgctggc
    33721 gaaagaaacc cgtccggacg tggttatcgg taccggccgc tccgacttcc cgaaccaagt
    33781 gaacaacgta ttgtgcttcc cgttcatctt ccggggcgcg ttggatgtcg gcgcaaccac
    33841 catcaacgaa gaaatgaaac gcgcctgcgt gtatgctttg gcagatttgg cgatggaaga
    33901 agtaaccgaa gaagtggttg ccgcttacgg taagaaattc gaattcggcg cggaatacct
    33961 gattcctact ccgttcgatt cccgcctgct gcctcgcgtc gctacggctg ccgccaaagc
    34021 agcgatggaa agcggcgtgg caacccgtcc gattgcagat ttggaagctt acgctgccaa
    34081 gttgggcgaa tggaagctgt aagccgtttg tggttaaaat gccgtctgaa ctgttttcag
    34141 gcggcatttt gttgtcagat tgataaatga aagatactgg aaaatgaaag agatgaaacc
    34201 tgtccgttat catattggcg atatacccga aacttcaaaa caaaccgccc ccggtcatga
    34261 cgacagggca gtgggtgttg acgatgattg ttttcatgat tcctttggtc aatatcgtta
    34321 tgttttttgt ttgggcgttc ggcagaggca accccaaccg tgccaatttc tgtaaagcac
    34381 tgttcttatt tactttacct tgttggttcg cttatcggtt tgattttcat attgcttata
    34441 ggtgggtctg catcgggtac gcattattaa tgtgccggct gattctgctt cgaagatttg
    34501 tatcgaatat gccaaatttt tttaaatttc ataccgttat cgaacggcat tggcaaaaac
    34561 cttatccggt tttgtctttt ctgctcaagc cgctttccgg gctgtttgcc aaaattgcgg
    34621 caaaatggcg ggcggatttt ttatcgggaa aacggcaaag cgaaaagctg tccgtgcctg
    34681 tggtcgtggt cggcaacatc cacgcgggcg ggacgggcaa aacgccgatt gccgccgcgc
    34741 tggtgtcggg cttgcaggaa aaaggtgtca aggtcggcat catcagccga ggctacgggc
    34801 gcaagagcaa ggcggtttat gtattgaatg ccgcgagccg tgcggaagat gcgggcgacg
    34861 agcctttgct gctgttccgt aaaacaggtg cgccgacggc agtgggcagc agccgtgtgg
    34921 aggcaggcag ggcgttgctg gcagcgcatc cggagcttga attgattgtg gcggacgacg
    34981 gtttgcagca ttacgccctg caacgcgatg tggaaatcgc ggtgtttccg gcggcggata
    35041 cggggcgtac ggatttggac ttgctgccca acggcaattt gcgcgaacct ttgtcgagat
    35101 tggaaagcgt ggatgcggtg gtggtcggcg gcagggcggc ggatggtttt atgccgtctg
    35161 aacatttgtt cggcagccgt attgaggcgg gtgcggttta ccgtttgaac cgtccgtctg
    35221 aaaaactgga tatttcgaca ttgtcaggga agcgcgtcgc agcggttgcc ggtatcgcca
    35281 ggccgcagcg tttcttcgat actctgacac acatgggcat ccgccttgac caaacggttg
    35341 ctctgcccga ccatgccgat attttcaatc gggatttgcc gcctgccgat gtggtgctgg
    35401 taactgaaaa agatgcggtc aaattttcag acggcatttg caccgataat gtttgggtgc
    35461 tgccggtttg tgcgataatc gaacctgatt tggcggagtt tgtgctggag cggttggagg
    35521 gtgtaccgaa ggccgtctga aagcgcggtt tggacgaagt gataacggac tggaataaga
    35581 acgccctacg ccgtcattcc cgcgcaggcg ggaatctaag tctcgaattt tcaggaatgc
    35641 ctaggagact tcagaaatcc caaatctccg gatttccact tgggcaggaa tgagaaagcc
    35701 ggttgtattt tttatctgca ttaatcattc gttaaaggag tggatatgaa gctgaaaacc
    35761 ttgttattgc ccttcgccgc actggcattg tgtgccaacg catttgccgc cccgcccggc
    35821 gacgcgtcgt tggcacgttg gctggatacg cagaatttcg accgggatat agaaaaaaat
    35881 atgattgaag gctttaatgc cggatttaaa ccgtatgcgg acaaagccct tgccgaaatg
    35941 ccggaagcga aaaaagatca ggcggcagaa gcctttaatc gttatcgtga gaatgttttg
    36001 aaagatttga ttacgcccga agtgaaacag gctgtccgca ataccttatt gaagaatgcc
    36061 cgtgaaatat acacgcaaga agaaattgac ggcatgattg ccttttacgg ttcgcctgtc
    36121 ggtcagtccg tggttgccaa aaatccgcgc ttaatcaaga aatcgatgag tgaaatagcg
    36181 gtatcttgga ctgcattgtc agggaaaatc gcgcgacatc atctgcccga gtttacggaa
    36241 gagttacggc gcatcatctg cggcggtata gtggattaaa tttaaatcag gacaaggcgg
    36301 cgaagccgca gaccgtacaa atagtacggc aaggcgaggc aacgccgtac cggtttaaat
    36361 ttaatccact ataaaaatcc cgatgcgggc tgcaagcagg ccggacagct tgggaaaagg
    36421 tatcagaagt aaataatagc cgcctgaaat attgcggagg gcatccgatt gattaaacca
    36481 tcaaacccga aagcaacctt atggaaaaaa aattcttaga catcctcgtc tgccccgtta
    36541 ccaaaggcag gctggaatat catcaggaca aacaggaatt gtggagccgt caggcgaagc
    36601 ttgcctatcc gattaaagac ggcattccct atatgctgga aaacgaagca cgagcgttga
    36661 gcgaagagga actcaaagca tgaccgaatt cgtcgtattg attccggcgc ggctggattc
    36721 gtcgcgcctg cccggaaaag ccttggcgga cattcacggc aaaccgatgg tcgtgcgcgt
    36781 tgccgaacag gcggcaaaaa gcaaagccgc gcgcgtcgtc gtcgccaccg atcatcccga
    36841 cattcagacg gcctgtcagg cgcacggtat cgaagtcgtc atgacttcaa accggcacga
    36901 aagcggcacg acgcgccttg ccgaagccgc cgccgcgctg aagctgccgc cgcatttgat
    36961 tgttgtgaac gtacagggcg acgagccgct gattgccccc gaactcatcg accgcaccgc
    37021 cgaagtactc gtcgaaaaca acgtccaaat ggcgaccgcc ggccacgaat tgcacgattt
    37081 cgacgaattg atgaatccca atgccgtcaa agtcgtcctc gacaaaaacg gcaacgccat
    37141 ctacttcagc cgtgccccga ttccctatcc gcgcgatgcg atgcgtgccg gaaaacgcga
    37201 aatgccgtct gaaaccgccg tcctacgcca tatcggcatt tacgcctacc gcgtcggctt
    37261 cctgcaacgc tatgccgaaa tgagcgtctc gccgctggaa accatcgaat cgctggaaca
    37321 actgcgcgtc ctgtggcacg gctaccccat cgccgtcgaa accgccaaag aagcccccgc
    37381 cgccggtgtg gatacgcagg aagatttgga cagggttcgc gctgtatttc agaccgtata
    37441 aaacaggttc aaagggaaaa gatatgcagc aacatattga aaagtggcaa cacttgagcc
    37501 gtgaagaaca gaaaatcctt gccgaagtat ggggtctcgt gcaaaacgat gatcaggagg
    37561 ttcactatga aatgctcaaa ttgaacgcac ctgatgaagt cagcggtgaa ttttggttca
    37621 gaatggcaga aacactcagc accctgccgc ccaatcgttc cctcgacctt agaatgaacg
    37681 gcggcaggct gtcgaccgcc gtatccatcc tttccgtcat gattgaagac aatcccgaca
    37741 taccgcagct ttgggcgtaa aaaattaccg ccctcaatta tttggtacac ggacacaaag
    37801 cccgtgccga cggtttggta caacagcccg acaaagcggc agaagccaac gaggaggaat
    37861 acctgaccaa agccctgtcg caaaacctgc tgtcaacatt ggatgccgca cttgcacgtt
    37921 ttcctgaaga cgcgtggttt caggaaatca aacaggatgc acaaaagcat tttgcttgag
    37981 gacgtggcgg tcaggaatat ttccattcag gaagaaaaga agtgcctaat tgggtataat
    38041 cagggtaaat cttattttat ttcaaaagat taatatttgt tttccgtttt tccttgacgg
    38101 tatcggaaaa gttgattata gttacagctt ccttaggagt aatggctgag aggctgaagg
    38161 cacttccctg ctaaggaagc atgtgggatc aacctgcatc gagggttcga atccctctta
    38221 ctccgccaga taaaaaaata gacgctgtgt tttacagcgt ctattttttt atgcaatttt
    38281 atagcgggtt ggcgcaaacc cggtatggta ttgccctgtc ttgattctga attttgttaa
    38341 tccgagatgt ttgccgttta tttttgcctc gttcaaacgg cggctctgat ctgcgcggtt
    38401 tctgtttgcc gtattcgcct acccgtaccg caaatgttat actgggaaaa attcaccgat
    38461 tgtattttac ggcgtatttg ccgataggat ggaagagaca aatgagcaga atccggcagg
    38521 cttttgccgc tttggatggc ggaaaggcat tgattcccta tattgcggtg ggcgatcccg
    38581 atattcggac aactttggca ttgatgcacg gcatggttgc aagcggtgcg gatattttgg
    38641 agttgggcgt gccgttttcc gatccgatgg cggacgggcc ggttattcag cgtgcggcgg
    38701 agcgggcgtt ggcaaacggg atttcgctgc gcgatgtctt ggatgtcgtc agaaaattcc
    38761 gtgaaaccga cacgcaaacg ccggttgttt tgatgggata tttgaatccc atacataaga
    38821 tgggttatcg ggagtttgct caggaagccg caaaggcggg ggtggacggc gtgttgacgg
    38881 tggattcccc catcgaaacc atcgattctt tatatcgcga gctgaaggat aacgaggtcg
    38941 actgtatttt cctgattgcg ccgacgacga cggaagaccg tattaaaacc attgccgagc
    39001 tggcaggcgg atttgtctat tatgtttcgc tcaagggcgt aacgggcgcg gcaagtttgg
    39061 atacggatga ggtttcgcgt aaaatagagt atttgcgtca gtatatcgat attcccatcg
    39121 gtgtcggttt cggcatcagc aatgcggaaa gcgcgcgcaa aatcggccgg gttgccgccg
    39181 cgatcattgt cggcagccgg attgtgaaag agattgaaaa caatgcgggc aacgaggctg
    39241 ccgccgtcgg tgctttggtc aaagagttaa aggatgccgt gcgctgattc tgtcgcgcat
    39301 tctaaatgtt ttaggagttg tccatgagct ggttagataa aatcctgcca cccaaaatca
    39361 agaatcgtgg gaaagacggt tcttccaatg ttcccgaggg tttgtggcgc aaatgcccgt
    39421 cttgttcggc aaccgtttat tcgaccgagt tgcagcagaa caatcaggtc tgcccgaaat
    39481 gcaaccacca caatccgtta tcggcgcgcc agcgtttgaa cctgcttttg gatgaggaag
    39541 gcagggagga agttgcgggt aatgtcaaac cgaccgatcc tttgaagttt aaagacagca
    39601 aaaaatatcc ggaccgtttg agtgcggcac gcaagctgac cggggaagat gacgcgctgg
    39661 tggtgatgaa ggggatgatg aacggtctgc ccgtcgtcgt tgccgcgttt gaattccgct
    39721 ttatcggcgg ttcgatgggt tcggttgtgg gcgagcgttt cgtacagggt gtccgccgtg
    39781 cggttgccga taattgtccg tttgtctgtg tggcggcttc cggcggcgcg cgtatgcagg
    39841 agggtgtaaa ctcgctgatg cagatgacga aaaccagtgc cgcgctgcat ttgctgacgg
    39901 aaaaacgcct gccgtttata tcggtgttga ccgatccgac tatgggcggc gtatctgcca
    39961 gcttcgcatt tttaggcgat gtcgtgcttg ccgaaccgaa cgcgctgatc ggttttgccg
    40021 gtccgcgcgt gattgagcag acggtgcgcg aaacgctgcc ggaaggcttc caacgcgccg
    40081 agtttctgct ggaaaaaggc gcgatcgacc agattgtcga ccgccgcgat atgaagcggc
    40141 gcatcagtga tttgattacg ctgttgtgcc gtcaggacaa agtttccgcc acctgatggc
    40201 tgatgaatcg aatgccgtct gaaaccgatg tttcagacgg catttttgtg tctggttatt
    40261 tgtcgtgcgg cttcatcgac ggggcatagc gtccggcacg ttctttcagg cgttgtacca
    40321 aaccttgcgt gtcggcgggt acgccgccct cgcagaatgc ctgatacagg acggcgcgca
    40381 atgcgtcgtt gcgcccaagt gtgccgccta tcggtttcca ttcggcgttt cggggctgta
    40441 tccagcggct gttgaccgta tcgccgtatc cgaatacttt gtaggaggaa agtttgccgt
    40501 tgccgaaccc gatggaggag agggcgcaga atatgccttc ggcagtcagg ttgtcgtagc
    40561 ctttgtcgga gcggatattg agaatgtagc ggatgctgcc gtcgggcgcg ggcataattt
    40621 gcaggctgtc gagcaggatt ttcggctgtt tgccgtaatt ttcatccaca taaatgtcaa
    40681 accagccgtc cgagtgcgca tcgggcagag gcggcagttc ggcggtatgt tctttaaatt
    40741 cgcgggcggc ggcttcttcg ggcgtttcgc ggtagcgggt gttgatcggc gtgtcttttt
    40801 ggctgaagcc ggcggcaagg gacgtgccga cagtcagggt cagaatcaga aggatggcgc
    40861 ggcgcataag tttctccaaa ttgaaaacgg ctttatttta tggtttggcg ggaagggctg
    40921 caagcaatcg gggtataatc tgacctgatt ttcattttaa agcggtgtcg aaccatgaac
    40981 agcgaaactt tagacgtaac cggattgaaa tgtcccctgc cgattttgcg ggcaaaaaag
    41041 gctttggcgc aaatgcggca gggcgaggta ttgaccgttc tggcgaccga cggcggcgcg
    41101 ccgggggact ttgaggcttt ttgccgccaa accggtcatg tgctgttgga ttcttccgaa
    41161 caggacggcg tgttcacact ggtcgtcaag cacaaataaa tgccgtctga aatgcggatg
    41221 tcctgattga tgttattgct gtcggcattt gtgcttgctg cgtgtaatgc ccaaaaccgg
    41281 caatccggtg tacggcatca ggattttgca cgcgtagccg tcgatttgtt tcggagtgcc
    41341 tgcgtcctga ctcaaggtag tttcgaaccg gtttccaagt ttgccgctgt cggacatttt
    41401 gtccctgtcg gaaaggaaga cctgtcccgt ctgccgcccg ctgttgcaga acccgatgta
    41461 caggcgttgt ggacgcttga acgcgatggc ggcatttatt atttgagcct gacgcgcgac
    41521 agttgcagtg tgaaagccga gcgggcagac agtgccgcgc ttttggaaca tttctcaggg
    41581 cttgtccgac agccgccgaa aaatgccaa
//
LOCUS       DBJGGI010000008        40707 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.008, whole genome shotgun sequence.
ACCESSION   DBJGGI010000008 DBJGGI010000000
VERSION     DBJGGI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40707
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.008"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            74..1198
                     /locus_tag="ACSIGJ_000461"
     CDS             74..1198
                     /locus_tag="ACSIGJ_000461"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HHQ1329422.1"
                     /translation="MLSKQISNLNSSSNKPKILSLFSGCGGLDLGFHQAGCETVWAND
                     FSHWACESFRKNIGDVIVEGDIEQINPNDPTIPDCDIILGGFPCQDFSMIWKQPGLEG
                     ERGNLYKSFLRFVNAKKPKVFVAENVKGLLTANKKKAIQQIITDFENCGYYVQAKLYN
                     FAEFGVPQFRERVLIVGVRLDTGFDFRHPEPTHNETGENGLKPYVTAGQAISNIPQNA
                     SNNELLKISDKTRRMLELIPEGGNFTDIPKDHPLYVKGMISHVYRRMHRNEPSKTIIA
                     AGGGGTWGYHFPEPRAFTNRERARLQSFPDDFEFVGSTTEVRRQIGNAVPPQGVVELA
                     KSILPIFSDNYEKVDLHEKLVEEKEILFHDRLSKIRGGKQ"
     gene            1195..2232
                     /locus_tag="ACSIGJ_000462"
     CDS             1195..2232
                     /locus_tag="ACSIGJ_000462"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease PLD domain-containing
                     protein"
                     /protein_id="HHQ1329423.1"
                     /translation="MNTVFSNIANAKITEKSLNAVWMDLFKSADEVLMATGYVSNDAV
                     VELHKILELNDHIQKIDLLVGMHYLEGFSHLQYDSLCKLNDFLRHEKRGAVYVSPFVK
                     FHGKMYSFKNYQKINGLIGSANLTCFWDSTERTYETMLHLNGKPAQILQADIQSTIHK
                     LGKNIQEVERPSKFIEHNSHLENCLGVQKIAPEQIRQLFAQTSEYHFSIPAKTEEKSN
                     LNVFFGEGRRDKRGFVKPRPWYEVELIVSKDITSQEGYPVLKSFTVITDDGWQFQCKT
                     SGDYSKNFRSENDLKTLGKWIKGRLESHGCLQNNEKITHETLREYGNDHFELRSTDNP
                     DVWLLSFKGKN"
     gene            2233..4122
                     /locus_tag="ACSIGJ_000463"
     CDS             2233..4122
                     /locus_tag="ACSIGJ_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z1 domain-containing protein"
                     /protein_id="HHQ1329424.1"
                     /translation="MLRTYLNQLTPPELADSVKNTVDGFMEKLSQTEPKIAQNVLLLG
                     NVQSGKTAQVLGVLSALADDGDHKVFLYLTTDSVDLQDQTVKRAKANLKNFIVLSEAD
                     DRSFMEVMKAENPILVVIKKNARVLKRWRNLFASQSSLKGYPLVIVDDEADAASLNTN
                     SDKPAKDASTINKLLNDIKNSCCQSLFIQLTATPQSLLLQHEESDWQPEFIHFFEAGE
                     KYIGGNFVFSDPPSYIVRFIDSELDDMKDESGEIAEGAKQALLSFLITCAEFALCDKA
                     NCNFALHPSYKIQDHQAFSKKIQAFLNDLVQAVNNGEDLAGSFKESYLDLQKTKPDIH
                     HFDEIYEKLTALLENKQISTLVVNSQTETDFDLEKGFNIIIGGNVIGRGLTIPKLQTV
                     YYSRTAKKPNADTFWQHSRIFGYDRDKSLLRLYIPFDVYYFFVQLNQANNLIIGQAKN
                     SGGNIQVIYPKNINPTRKNVLKFDSINQIVGGVNYFPLHPNEDNLSEINKILPSILKD
                     EIQSDLYQIDIEDLFLVLDKLGRYVPDDWNKEKFIAGVEALKAQRPSFKTYVLIKTGR
                     KLSRATGTMLSEDDRKLGEKYPNDLFLTLYQVVGNKDKGWQGKDFWLPNIKLPHNGLV
                     YQSAK"
     gene            complement(4185..5249)
                     /gene="hemE"
                     /locus_tag="ACSIGJ_000464"
     CDS             complement(4185..5249)
                     /gene="hemE"
                     /locus_tag="ACSIGJ_000464"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HHQ1329425.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTGLATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHESDIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLYKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGNQVALQGNFDPSALFGTPESIRTEVARILTGYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(5324..6541)
                     /locus_tag="ACSIGJ_000465"
     CDS             complement(5324..6541)
                     /locus_tag="ACSIGJ_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HHQ1329426.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVLNIPEKMQRSGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQMADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADSWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSIPARLVLAKVFDETAQSQKAEAQRNLVLA
                     SVAGENRPSAETR"
     gene            complement(6538..7872)
                     /locus_tag="ACSIGJ_000466"
     CDS             complement(6538..7872)
                     /locus_tag="ACSIGJ_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HHQ1329427.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAACALVLAALGLGASGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQSAIQSELDRLDSGVKANGEQILMTQKAYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQARNEAVSASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNGEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKTLDVRMTADDGLKTSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKAIEAPSLPSERKPEQPAKKQPAPEKAGRSPSAKGERA"
     gene            complement(7884..8648)
                     /locus_tag="ACSIGJ_000467"
     CDS             complement(7884..8648)
                     /locus_tag="ACSIGJ_000467"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HHQ1329428.1"
                     /translation="METAKPVMLIVRPSGRAKDDVEVCRRAGWQAEVLSPIEIETDEA
                     SLKRLPEMYARADAVFWVSPAAVETAVPYLNLSDGIKAHIAVGQGSRRALARYAGAGV
                     FAPEDGNDSEAVLRLPVWNSLPEGARVLSVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVQSLFAQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSYSSISVSDGMVFPGTSNQ"
     gene            complement(8666..9088)
                     /locus_tag="ACSIGJ_000468"
     CDS             complement(8666..9088)
                     /locus_tag="ACSIGJ_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Gly1ORF1"
                     /protein_id="HHQ1329429.1"
                     /translation="MKKMFLSAALLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRATMKFQNGEFMYTVWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFSR"
     gene            complement(9370..10338)
                     /locus_tag="ACSIGJ_000469"
     CDS             complement(9370..10338)
                     /locus_tag="ACSIGJ_000469"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HHQ1329430.1"
                     /translation="MVELQEIFDVSYGSKLDLNKMSSFNPTINFVGRSGKNNGVTASV
                     DLLKNTKPYPAGLLTVALGGSVLSTFLQNKPFYTAQNVAVLNPKTEMTEQQKLFYCAA
                     IFANAYRFSACGREANRTLRQLFVPSLDEIPSWVESVNLNPSAGVTEPKLKEPLDLPV
                     VRQSKRLDEIFTIQNGIAATKLKEFEQRQKDTVVYIRPASTQARTLRSYIARDSVDEK
                     HIFPCHTLFTSTNGEGSHTYSYVSTCEFVANSDVAVLTPIQSDMPIEVKLYYAKCITA
                     NRYLFSYGRKPKGEKLKSIMLPYFDQQEDFDYICRFIHTLLFSNNL"
     gene            complement(10338..11162)
                     /locus_tag="ACSIGJ_000470"
     CDS             complement(10338..11162)
                     /locus_tag="ACSIGJ_000470"
                     /inference="COORDINATES: protein motif:HMM:NF014442.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HHQ1329431.1"
                     /translation="MFALAASNMILRGDGKANLHQSSCFMTDFQDLIKNPKPETGLKR
                     PNVGFLNPPYAQSKSDAELHELYFVKEMLDMLAEGGTGIAIIPVSCVIAPSKAKSEIV
                     KYHRLKAVMSMPSELFYPVGTVTCIVVFEAHKPHFQTVVIDPDTQEEISTKKACRKTW
                     FGYWRDDGFEKTKHLGRIDLYDRWQGIKARWLEHYLNNEVHTGESVTAFVTDNDEWVA
                     EAYLETDYSKITRADFEQVVREFALFQLLGAEVGPTENLDNESYEDDDNNDFGDDE"
     gene            complement(11192..11833)
                     /locus_tag="ACSIGJ_000471"
                     /pseudo
     CDS             complement(11192..11833)
                     /locus_tag="ACSIGJ_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(12002..12322)
                     /locus_tag="ACSIGJ_000472"
     CDS             complement(12002..12322)
                     /locus_tag="ACSIGJ_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HHQ1329432.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDAEGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLGKEAAEQGLIWFAEHVADARAHPGKHPNIDLLENVVQSGETLLLKP
                     LAAQ"
     gene            complement(12345..12971)
                     /gene="upp"
                     /locus_tag="ACSIGJ_000473"
     CDS             complement(12345..12971)
                     /gene="upp"
                     /locus_tag="ACSIGJ_000473"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006223 - uracil salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HHQ1329433.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLTTELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPISYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKEKGCRNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSRLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(13077..13388)
                     /gene="grxD"
                     /locus_tag="ACSIGJ_000474"
     CDS             complement(13077..13388)
                     /gene="grxD"
                     /locus_tag="ACSIGJ_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HHQ1329434.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVAVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(13489..13704)
                     /locus_tag="ACSIGJ_000475"
     CDS             complement(13489..13704)
                     /locus_tag="ACSIGJ_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hemolysin"
                     /protein_id="HHQ1329435.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYCRSQH"
     gene            complement(13730..14509)
                     /locus_tag="ACSIGJ_000476"
     CDS             complement(13730..14509)
                     /locus_tag="ACSIGJ_000476"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HHQ1329436.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAVAEAHEHIYCTIGVHPDSKEAEEFSIAEMAEAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLAILKECKTNSGVIHCFSEDVGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRVV"
     gene            complement(14514..14864)
                     /locus_tag="ACSIGJ_000477"
     CDS             complement(14514..14864)
                     /locus_tag="ACSIGJ_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HHQ1329437.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTDDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPASTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGSTISGSRPTFTM"
     gene            complement(14868..15845)
                     /locus_tag="ACSIGJ_000478"
     CDS             complement(14868..15845)
                     /locus_tag="ACSIGJ_000478"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HHQ1329438.1"
                     /translation="MIYPWHQEQWRQIAEHWTSRPNAWLFVGKKGTGKTAFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGRGSHPDFYEITPLADEPENGRKLLRIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKKVLPAPSHGEALAYLRDRGVAEPEERLAFHSGAPLFQEEGELRELRAKLLEI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENR"
     gene            complement(15879..17009)
                     /locus_tag="ACSIGJ_000479"
     CDS             complement(15879..17009)
                     /locus_tag="ACSIGJ_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HHQ1329439.1"
                     /translation="MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKI
                     TRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGAT
                     ALVFRAITSKIPKFESLNLPPALKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
                     SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDR
                     ETMDYAIAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQ
                     RLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQL
                     YEKGEISLQDALKNADSAHDLRLAVQLRSRRAQSSDPDLELL"
     gene            17177..17878
                     /locus_tag="ACSIGJ_000480"
     CDS             17177..17878
                     /locus_tag="ACSIGJ_000480"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HHQ1329440.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGRFSAYEGELAGK
                     RMVLALSGIGKVNAAVATAWLIRQFAPDCVINTGSAGGLGKGLKVGDVVIGTETEHHD
                     VDVTAFGYARGQVPQLPARFASDGILIETAKRAARTFEGAEVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFEEFLKTA
                     AASSAKMVAEIVKSL"
     gene            18020..19813
                     /gene="lepA"
                     /locus_tag="ACSIGJ_000481"
     CDS             18020..19813
                     /gene="lepA"
                     /locus_tag="ACSIGJ_000481"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HHQ1329441.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHKG
                     RELASKMRELIPRQMFDIAVQAAIGSRIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            19959..20978
                     /gene="lepB"
                     /locus_tag="ACSIGJ_000482"
     CDS             19959..20978
                     /gene="lepB"
                     /locus_tag="ACSIGJ_000482"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HHQ1329442.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDIVEYRDKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIDQSGLNHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(21083..21190)
                     /locus_tag="ACSIGJ_000483"
                     /pseudo
     CDS             complement(21083..21190)
                     /locus_tag="ACSIGJ_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(21414..21563)
                     /locus_tag="ACSIGJ_000484"
     CDS             complement(21414..21563)
                     /locus_tag="ACSIGJ_000484"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329443.1"
                     /translation="MDWQAVKTGKTKHADNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(21609..22516)
                     /locus_tag="ACSIGJ_000485"
                     /pseudo
     CDS             complement(21609..22516)
                     /locus_tag="ACSIGJ_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708052.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(22825..23757)
                     /gene="cysK"
                     /locus_tag="ACSIGJ_000486"
     CDS             complement(22825..23757)
                     /gene="cysK"
                     /locus_tag="ACSIGJ_000486"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HHQ1329444.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTKGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEAAEKAGKINKNTVIVEATSGNTGIGLAMVCAARGYKLAITMPESMSKER
                     KMLLRTFGAELILTPAAEGMAGAIAKAQSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWNDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSIAKVPNEAAFETARAMAEKEGILAGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(23953..24558)
                     /locus_tag="ACSIGJ_000487"
     CDS             complement(23953..24558)
                     /locus_tag="ACSIGJ_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329445.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIALWLGISVL
                     GVKLMPGMWGMTRAAPLFIPHFYLTLGSIFFFIGYWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGVLAAMLMQVAVTAMLLAEIGR"
     gene            complement(24555..25406)
                     /gene="dapF"
                     /locus_tag="ACSIGJ_000488"
     CDS             complement(24555..25406)
                     /gene="dapF"
                     /locus_tag="ACSIGJ_000488"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HHQ1329446.1"
                     /translation="MKTLKFTKMHGLGNDFMVIDAVSQDFTPEDVPIAAWADRFRGVG
                     FDQLLVVGCSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     AKGIIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSRIEPHRQFPERVNVGFMQVVGRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVVLPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            25543..25857
                     /locus_tag="ACSIGJ_000489"
     CDS             25543..25857
                     /locus_tag="ACSIGJ_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329447.1"
                     /translation="MSRLFLLLFPWATLAACTRNIPASPAVSNCAHLHNRTVLYFDAH
                     GKPADSTAKGGFYRTVEIHDTNRILIQDFNSTGNALVKPYWSDCRKLSVFPDKHLTMS
                     HP"
     gene            25854..26522
                     /locus_tag="ACSIGJ_000490"
     CDS             25854..26522
                     /locus_tag="ACSIGJ_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HHQ1329448.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRISSYTAIGLMLGLIGQLGISLDQTRVLQNILYTASNLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSATTGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(26595..28718)
                     /gene="pnp"
                     /locus_tag="ACSIGJ_000491"
     CDS             complement(26595..28718)
                     /gene="pnp"
                     /locus_tag="ACSIGJ_000491"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HHQ1329449.1"
                     /translation="MMFNKYVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVFVA
                     VTTNKEVKEGQDFFPLAVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRP
                     LFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYING
                     VYVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAIN
                     AINEFADEVNPEVWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWN
                     AVKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTG
                     VLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEV
                     GRMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCL
                     SLLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQ
                     MDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKISQDKI
                     RDVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEEITAEVEVGK
                     VYEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEV
                     DDRGRVRLSIKALLDAPVREENAAE"
     gene            complement(28952..29815)
                     /locus_tag="ACSIGJ_000492"
     CDS             complement(28952..29815)
                     /locus_tag="ACSIGJ_000492"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HHQ1329450.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDAPI
                     PGKGEILTQISNFWFKKLAHIMPNHFTGDTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMREAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRDKAVRLYTEAAEYAKSRGIIICDTKFEFGLDEKGTLTLMDE
                     VLTPDSSRFWPADQYEVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIRKTVEK
                     YQEALTLLTQD"
     gene            30008..30871
                     /gene="rfbD"
                     /locus_tag="ACSIGJ_000493"
     CDS             30008..30871
                     /gene="rfbD"
                     /locus_tag="ACSIGJ_000493"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HHQ1329451.1"
                     /translation="MRILLTGSKSQLAHCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     VFDGKGKIPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYAGGKSVSWYE
                     FARHIFQTALQQDASFPVPELKAVSDEGSAAAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
     gene            complement(31243..31476)
                     /locus_tag="ACSIGJ_000494"
     CDS             complement(31243..31476)
                     /locus_tag="ACSIGJ_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HHQ1329452.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQDEYGFGLEVVDVDENPVLE
                     EKYNELIPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(31494..31628)
                     /locus_tag="ACSIGJ_000495"
     CDS             complement(31494..31628)
                     /locus_tag="ACSIGJ_000495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329453.1"
                     /translation="MPSELFRRHFCCFWQDLSIIFLEDIVFSQIDHDSAASKALACAN
                     "
     gene            complement(31642..31842)
                     /locus_tag="ACSIGJ_000496"
     CDS             complement(31642..31842)
                     /locus_tag="ACSIGJ_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HHQ1329454.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(32014..32889)
                     /gene="xerD"
                     /locus_tag="ACSIGJ_000497"
     CDS             complement(32014..32889)
                     /gene="xerD"
                     /locus_tag="ACSIGJ_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HHQ1329455.1"
                     /translation="MENGLIDRLLETLWLDRRLSRNTLDSYRRDLEKIARRLSLCGRT
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYVWMEREGMRADNPTRLLKPPKIDR
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITTLGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRSCDALFVSQKKTGISRQLA
                     WMIVKEYAGRAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVRLHSVVKEHHSRN"
     gene            complement(32941..33381)
                     /locus_tag="ACSIGJ_000498"
     CDS             complement(32941..33381)
                     /locus_tag="ACSIGJ_000498"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HHQ1329456.1"
                     /translation="MDMKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIVREWRKVKVAGHAQEVLETLSR"
     gene            33702..34640
                     /locus_tag="ACSIGJ_000499"
     CDS             33702..34640
                     /locus_tag="ACSIGJ_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HHQ1329457.1"
                     /translation="MSIRTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIYPADTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRLKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            34769..35062
                     /gene="hfq"
                     /locus_tag="ACSIGJ_000500"
     CDS             34769..35062
                     /gene="hfq"
                     /locus_tag="ACSIGJ_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HHQ1329458.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENKPQAAPASTLVQVETVQQPAE"
     gene            35147..35713
                     /locus_tag="ACSIGJ_000501"
                     /pseudo
     CDS             35147..35713
                     /locus_tag="ACSIGJ_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            35767..36141
                     /locus_tag="ACSIGJ_000502"
     CDS             35767..36141
                     /locus_tag="ACSIGJ_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HHQ1329459.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRFFLLWKNPEKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLFAYIALGMVMMRARPRSTKFYTV
                     YLLAMCCIACIVYLAKTKVLPF"
     gene            36151..36645
                     /gene="folK"
                     /locus_tag="ACSIGJ_000503"
     CDS             36151..36645
                     /gene="folK"
                     /locus_tag="ACSIGJ_000503"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HHQ1329460.1"
                     /translation="MNNRHFAVIALGSNLDNPAQQIRGALDALSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFINAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFILGKYGKVVELSKRLGNQGIRL
                     LPDR"
     gene            complement(36773..37252)
                     /locus_tag="ACSIGJ_000504"
     CDS             complement(36773..37252)
                     /locus_tag="ACSIGJ_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HHQ1329461.1"
                     /translation="MPESIFKQISSDILRLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVETGILLIEDESRNLRLSWVGSARELDRKDNAYKRALSALSRKL
                     GRCKDKLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVLQI"
     gene            complement(37472..38209)
                     /gene="ubiE"
                     /locus_tag="ACSIGJ_000505"
     CDS             complement(37472..38209)
                     /gene="ubiE"
                     /locus_tag="ACSIGJ_000505"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HHQ1329462.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTIHTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTYKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGRLIAKDADSYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(38252..38620)
                     /locus_tag="ACSIGJ_000506"
     CDS             complement(38252..38620)
                     /locus_tag="ACSIGJ_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase-like
                     domain-containing protein"
                     /protein_id="HHQ1329463.1"
                     /translation="MNNIPVEIRLLKNRAVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            38740..40359
                     /gene="mnmC"
                     /locus_tag="ACSIGJ_000507"
     CDS             38740..40359
                     /gene="mnmC"
                     /locus_tag="ACSIGJ_000507"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HHQ1329464.1"
                     /translation="MDNLVWDGIPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDSECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKSDKPWFALPQTSERKKPEHVLVIGAGIAGASTAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTGQTELLLAGYGYTKRLLGHILPDSDTWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITSAEAEKIAGIPLNTPYAEPLCGLYWQHGVW
                     LNPPAFVRTLLSHPLIELYENTTLTGISHDGEKWIASTPNGTFTATHIIYCTGAHSPC
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SNTGWNEAEEASNRQALAHLNPALAESLFAANPNPQKHQGHAAIRCDSPDHLPVVGAL
                     GDIAAMRQTYAKLALDKNYRIDTTCPYLPNAYTNTAHGTRGLATAPICAAAVAAEILG
                     LPHLFSQRLRHALHPNRTVIRAIVRRQNLIP"
     gene            complement(40356..40505)
                     /locus_tag="ACSIGJ_000508"
     CDS             complement(40356..40505)
                     /locus_tag="ACSIGJ_000508"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329465.1"
                     /translation="MDSKGNLLGIPYSLFWEENVMGVFDILPSGLVCLFEYDFLWSGR
                     HAAKA"
BASE COUNT        10341 a        10953 c         9350 g        10063 t
ORIGIN      
        1 cgttcagagc atagttcacc tttaaaatcc atacaaggat ttttatcaaa tttatgcata
       61 ggagtgaaat cctatgctat ctaaacaaat ctcaaatctt aattcttcta gtaacaaacc
      121 aaaaatccta tctctatttt caggatgtgg cggtttggat ttgggctttc atcaagctgg
      181 ttgtgaaact gtttgggcga acgatttctc ccattgggct tgcgaaagtt tccgtaaaaa
      241 tatcggcgat gtcatcgtag aaggtgatat tgaacaaatt aatccgaatg atccaactat
      301 tcccgattgc gacatcattt taggcggatt cccttgtcaa gatttttcca tgatttggaa
      361 acagccgggc ttagagggtg agcgcggcaa tctttataaa agctttttac gttttgtaaa
      421 tgcaaaaaaa ccgaaagttt ttgttgctga gaatgtgaaa ggtttattga ctgccaacaa
      481 gaaaaaagcc atccagcaaa ttattaccga ctttgaaaat tgcggttatt acgttcaggc
      541 gaagctgtat aactttgcag aatttggcgt acctcaattt cgtgaacgtg tgctgattgt
      601 cggagtacgt ttggatacag gatttgattt tcgccatccg gaaccgacgc acaatgaaac
      661 tggcgaaaac ggcttaaaac catatgtaac agcaggtcag gccatatcca atattccaca
      721 aaatgccagt aataatgaat tactgaaaat cagcgataaa acacgccgta tgttggaatt
      781 aattcctgaa ggtggaaatt ttaccgatat tcctaaagat catcctttat atgtgaaagg
      841 tatgattagc cacgtttatc gtcgtatgca tcggaacgag ccatcaaaaa caattattgc
      901 agcaggtggc ggtggtactt ggggctatca cttccctgaa ccgcgtgctt ttactaatag
      961 agaacgagca aggcttcaaa gttttcctga tgattttgag tttgtcggat caacaactga
     1021 agtacgtcgc cagattggta atgctgttcc tcctcagggc gtggttgaac tggcaaaaag
     1081 cattttaccg attttttcag acaactatga gaaagtagat ttgcatgaga aattagtcga
     1141 agaaaaagaa attttattcc atgaccgact aagcaaaatt cgagggggaa aacaatgaat
     1201 acagtttttt ccaatatcgc caatgccaaa atcactgaaa aatctcttaa tgcggtttgg
     1261 atggatttat ttaaatcagc ggatgaagtg ttgatggcga caggttatgt atctaatgac
     1321 gcagtggttg agttacataa aattttggag ctaaacgatc atattcaaaa aatagattta
     1381 ttggtcggaa tgcattattt ggaaggtttt agccatttac aatatgacag cttatgtaaa
     1441 ctaaatgatt ttttgcgaca tgagaaaaga ggagccgtct atgtttcccc ctttgtgaaa
     1501 tttcacggca aaatgtattc ctttaaaaat tatcaaaaaa tcaatggatt aattggttct
     1561 gctaacttaa cgtgtttttg ggatagcacg gaacgtactt atgaaactat gttgcatttg
     1621 aacggtaagc ctgcacagat tttgcaggca gatattcaaa gtactattca taagctaggc
     1681 aaaaatatcc aagaagttga aagaccaagt aaatttattg aacataatag ccatttagaa
     1741 aattgtttgg gtgttcagaa aattgcacca gaacagattc ggcaattatt tgcccaaacg
     1801 tcagaatatc atttctcaat tccagctaaa accgaagaaa aaagtaattt gaatgtattt
     1861 tttggagaag gtcgacgaga taaacgaggg tttgtcaaac cacgaccttg gtatgaagta
     1921 gagttgattg tatccaaaga catcacttca caagaagggt atccggttct gaagtctttt
     1981 acagttatta ccgatgatgg ttggcaattt caatgcaaga catcggggga ttactccaaa
     2041 aacttccgct cagaaaatga tttaaagaca cttggtaaat ggattaaagg tcgtttggaa
     2101 agtcatggct gcctgcaaaa taacgagaag attacgcatg aaaccctacg cgaatacggt
     2161 aacgatcatt ttgagttacg ttcaaccgat aatcctgatg tttggctgct ctcatttaag
     2221 gggaaaaact aaatgttgcg aacctacttg aaccaattga cccctcccga attagctgat
     2281 tctgttaaaa ataccgttga tggttttatg gaaaaattgt cacaaaccga gccgaaaatc
     2341 gcccaaaatg ttttattgct gggcaatgtt caaagtggca aaacggcaca ggttttgggt
     2401 gtattaagtg cgctggctga tgatggcgac cacaaagttt ttttgtatct tactactgac
     2461 agcgtagatt tacaggatca aacagtcaaa cgagccaaag ccaatctgaa aaactttatc
     2521 gtattgtctg aagctgacga ccgaagtttt atggaagtaa tgaaggcaga gaaccccatc
     2581 ttggtcgtga ttaaaaagaa tgcccgtgta ttgaaacgtt ggcgtaattt gtttgccagt
     2641 caaagtagcc tgaaaggcta tcctttggtt attgtggacg atgaagccga tgctgccagc
     2701 ctaaatacta attctgataa gccggctaaa gatgccagta ctatcaataa actgctaaac
     2761 gacatcaaaa attcctgttg tcaaagcctg tttatccagt tgactgccac gcctcaatcg
     2821 cttttattac aacatgaaga atctgattgg caacctgaat ttatccactt ctttgaagca
     2881 ggcgaaaaat acatcggcgg caactttgtc ttttctgatc caccaagcta tatagttcgt
     2941 tttattgata gcgaattgga tgatatgaaa gacgaaagtg gtgaaattgc cgaaggagca
     3001 aaacaggcat tgcttagttt tctaataacc tgtgcagaat tcgcgctatg tgataaggct
     3061 aactgtaatt ttgcactaca tccaagctat aaaattcaag atcatcaagc cttttcaaag
     3121 aagatccaag cctttttaaa tgacttagtg caagcagtca ataatgggga agaccttgca
     3181 ggtagcttta aagaaagtta tttggatttg caaaagacca agcccgatat tcatcacttt
     3241 gatgaaatat acgaaaaatt gacagcactt ttagagaata aacaaatctc tactcttgtc
     3301 gttaactcgc agacagaaac agattttgac ttagaaaaag gtttcaacat cattattggt
     3361 ggtaatgtga ttggtcgtgg cttgactatt ccgaaactac aaacggttta ttatagtcgc
     3421 actgccaaaa aaccgaatgc agatactttc tggcaacatt cgcgtatttt tggatatgac
     3481 cgcgataaat cattattacg gctttatatt ccgttcgatg tctattactt ctttgtacaa
     3541 ctcaatcaag caaataatct gattattggg caggcaaaaa attcaggcgg caatattcag
     3601 gttatttatc cgaaaaatat caatccaact cgaaaaaatg tattgaaatt cgatagtatc
     3661 aatcaaattg tgggtggtgt gaattatttt ccactccacc caaacgaaga taatttatcg
     3721 gagattaaca aaattttgcc ttctattttg aaagatgaaa ttcaatctga tttatatcaa
     3781 atagatatag aagatttatt cttggttcta gataaattgg gtcgttatgt tccagatgac
     3841 tggaataagg aaaaatttat tgctggtgta gaagcattaa aagcacaacg tccaagtttt
     3901 aaaacctatg ttttgatcaa aaccggacgt aaactttctc gagcaaccgg cacaatgctt
     3961 tcggaagacg accgtaaatt aggagaaaaa tatcccaatg atttattcct tacgctttat
     4021 caagtagtag gaaataaaga caaaggttgg cagggaaaag atttttggtt acccaatatc
     4081 aagctaccac acaatggctt ggtgtaccaa agtgcaaaat gaagcatatg ccgtctgaaa
     4141 acttccgatt cttcagacgg catatcgggc ggtttcctgc cggcttaccc gccgtgatac
     4201 tgccgcgaca gctcgtgtac ggtgtcgact aagattttgg cgtgttcggg gtcggcgtgt
     4261 tggttgatgc cgtgtccgag gttgaagaca tggccgctgc cgtgtccgta gccggtcagg
     4321 atacgtgcga cctcggtgcg gatggattcg ggcgtaccga agagggcgga cgggtcgaag
     4381 ttgccttgca gggcgacttg gttgccgacg cggcggcgtg cttcgccgat gttgcacgtc
     4441 cagtccaagc ccaatgcgtc tgcgccgatt tgggccatgc tttccagcca cagcccgccg
     4501 cctttggcaa acacgataac aggcacgcgg cgaccttcgc tttcacgttt gagtccggca
     4561 acgatttggc ggatgtattt gaggctgaac tctttaaacg ccgcgtcgct caacacgccg
     4621 ccccaagtgt cgaaaatctg caccgcctgc gcgcccgcgt cgatttgggc gttgaggtag
     4681 gcggtaacgg cttgggcgtt ggtatcgagg attttgtaca gcaaatcggg gcgcgagtac
     4741 atcatggttt tgatggtgcg gaattctttg ctgccgccgc cttcgaccat ataacaggcg
     4801 agcgtgaacg ggctgccgga gaagccgata agcggtacgc ggccgtccaa tgctttacgg
     4861 atggaagtta ccgcgtcgaa aacgtattgc agtttttcca tgtcgggaac gtgcagcttg
     4921 gcgatgtcgg attcgtgttg caaggcgcgt ttgaatttcg ggccttcgcc ttcggcaaaa
     4981 tacagcccca agcccattgc atcgggaacg gtcaagatgt cggaaaacaa aatcgccgcg
     5041 tccaaatcga agcgttccaa aggctggatg gtgacttcgg tcgccaatcc ggtgtttttg
     5101 cacaaatcga ggaagctgcc cgctttcgcg cgtgtggctt tgtattcggg cagataacgc
     5161 cccgcctggc gcatcatcca aatcggtgtg tattcgacgg gctgtttgag cagggcgcgg
     5221 aggaaggtat cgttttttaa aagagtcata cgggttttct gtcagtctgt ttgtcaagtg
     5281 aaatggtttt cagacggcat ttcaacgatg ccgtctgaag gtttcaacgg gtttcggcgg
     5341 aagggcggtt ttccccggca acgcttgcca aaaccaagtt gcgctgtgct tcggcttttt
     5401 gcgactgtgc ggtttcgtca aaaacctttg ccaacaccaa acgcgccgga atactcggct
     5461 tcagtgcaat actcgcttca aggtagcctt ttgccttacc ccaaagtttg cggccgtagg
     5521 cgagccggcc gagatacatc agcagaagcg cgttatcggg ctgttctttc agccaagaat
     5581 cggcaaaatc gatggctttc tgctgttcgc gctcgcccaa aaagcgcacg ctttcgacaa
     5641 aggcttccaa aagctcgggg cggcggttgt gcggataatg ctgtttgacc catttgaccg
     5701 catcggcata cagtcccaaa cgttcgtact tttccgcaac cgatacgctc aattccccgt
     5761 ttttgaggct gtcgggaatc cgcttcaggc aggttttcaa agcggcggca tcggcagcat
     5821 ccgccatctg gcggcggtat gcccaatttt gataccgttc catttccgat ttgcccaacg
     5881 cgcccgcctt ggaaagtttt tcggtttttg ccagaacctg caacgcatcg ccccgatcga
     5941 aggcgtaacg aagttgcaga cgcacgaggc gcgtaaggtt ggcattcatc ttcgccgccg
     6001 catgaagatt ggcttccgcc gcttcgtaat cgcgccggtt cagtgccgat tccgccagca
     6061 gaagatagcg ggaaagctgc tgtttttccg gcagtttggc gatttccgca agataacggt
     6121 cgcgcagctc gatattttcc atctgtcctg ccgcgtgcgc gcccagcatc aatgccaaag
     6181 tccgattgtc tccggcctct ttgttgccca acactcgaga ggcttcgagt tccgcctttt
     6241 caaaacgccc ttcgaaatac gccaaacctg ccttattcaa ggcaagcgcg gccttgcggc
     6301 ctttccgcgc cgaaccggaa cgctgcatct tttcggggat attaagtacg ccgatgatga
     6361 atttaaacag gaaataccac accacgacgg caatcagcga acctaacaca aaagcgtgca
     6421 ggttgattct gagcatggtc tgtccgagta cgatatacac gtcgccggtg taaatgcccg
     6481 aagccagcgc cagtccgacg gcggcggcaa acaggacgac aatccagact accgttttca
     6541 tgcgcgttct cctttagcgg acggcgaacg ccctgccttt tccggtgcgg gctgtttttt
     6601 tgcaggctgt tccggtttgc gttccgaagg cagggacggt gcttctatgg ccttcacatc
     6661 cgatgcggca gccggttcgg aagctgtgtt ttcgtttgcc ggttcggaag ccgcctgttc
     6721 cgcttcttga ttttccgccg ccgtcatgcg cgtaccgtcg cgataggcgc ggacggcatt
     6781 taaactggtc ttcaagccgt catccgccgt catgcgtaca tccagtgttt tcaattccgc
     6841 cagttctttc agccacgact gcgtggcggg cgacttggca tcgaaatact gtctgacggc
     6901 ggcttcggca ttgttcaaat cgccctgata gacttcgccg ttgcgctgca tcaatgcggt
     6961 gcgcgcatcc aacaggcgca gacgcaggtt ttcacgcaca aaatatgcct gttcgggaga
     7021 aatcagcatg gcatcgttgt tttccaaacg gcggatttcg accagcccct tcaatgtgcc
     7081 gagggatttt tcccatacgt tctgccacca tgaagcggaa acggcttcgt tgcgggcctg
     7141 tacgcccggt ttcaatacgc cgtccaaaat cagcggcagt ccggatacgg cggtttccaa
     7201 ccggtcgagg cgcaatgccg tgccggaaat atcgacatag ggacggtttt tcagttccgc
     7261 caaatcgctg ctgaccgcct gtttgatcgg cagaagctct gcctgatcga aacgggacag
     7321 gcggctgtcg atatgctcca acacgccgac tgccgtttgg atattgccgg tcaacaccag
     7381 ctgttgcgcc gccagattga gtatggtctc ggtttcgtcc accagccaat cggcacgccc
     7441 tttggtcagc tcgcgatagg ctttttgcgt catcaggatt tgttcgccgt tggctttgac
     7501 accgctgtcc aaacggtcga gttcggactg tatggcactt tggcggttga ggttgtcttt
     7561 gagcagggcg gcgttttccg actcgcccaa ggcggctttg tcgattttct ggttgaatgc
     7621 cagctcttgg tttttcaaga cattctgtcc ttggacaaac aaaaaaccac ttgcacccaa
     7681 acccaatgcc gccaatacca aggcgcaggc cgccaaagcg ttgctgccgg actgtttgac
     7741 gataaaagga gcggcgggaa tgtgtacttc tgtttcgttt tctttgcgtg gggaagaggt
     7801 ttcagacggc atttcttttg atgcctgtat ctcgcgtacg ggttcggatg atttgttttc
     7861 aggttcgccc accgttttgc tccttattgg tttgaggttc cgggaaagac catgccgtct
     7921 gaaacggaaa tggaagaata ggaaagcgcg gcttccagcg tagggacggt ttcgaccgaa
     7981 cacacgcctt cgcgcttcaa tgcctccgca atgcgcggat gatgggtaaa gtatagcaag
     8041 gatttgaaga atcgggaaaa ttgcggcgga agctgtgcaa acagcgactg cacaagttct
     8101 gtggacgtaa tataggcggc ggcaatattt tcggtttgga aattttgaaa gttcaaaggt
     8161 ttatgccgtc tgaaatagac ttctgccacc tccgtccgaa aacctttctc ctgcaaggca
     8221 ttcatcagaa aatcccgccc gccgtgtccg cgcacagaca atacgcgcgc accttcgggc
     8281 agactgttcc aaaccggcag gcgcaaaacc gcctcgctgt cgttgccgtc ttcaggtgca
     8341 aacacgcccg cgcccgcata acgcgccaag gcacggcggc tgccttgccc tacggcaatg
     8401 tgcgccttta tgccgtctga aaggttaagg tacgggacgg cggtttcaac ggcggccgga
     8461 ctgacccaaa acacggcatc cgcacgggca tacatttccg gcagccgttt caggcttgct
     8521 tcgtcggttt cgatttcaat cggactcaac acttccgcct gccatccggc acgacggcag
     8581 acttccacat cgtccttcgc cctgcccgac gggcggacaa tcagcatgac gggcttcgct
     8641 gtttccatcg ccttatccgc cccgattacc gggaaaaatc gtcatcgttg aaattcgcct
     8701 gcgcggtttt cggcgtacag cctacccgtg cgacttcctt gcccctacgc tccacaacga
     8761 caccgctgct ttccgtatga gtcacggaat cgaagcctgt ccatacggtg tacataaatt
     8821 cgccgttttg gaatttcatc gttgcccggc gtccgctgcc catgccttgc cacctgtcgg
     8881 aacgccccaa caggtcagct ttgctgttgc gtatggcaat ttcttttttt gccgcactgc
     8941 cgaacgaata ttcgtaaaga ttgcggttga ttttttgaac ttctatgtat ttgttgttgt
     9001 ccgttttaca ggaaaatacc gtatccgccc acacggtttg ggcggcagcc gacagaagca
     9061 atgcggcaga aaggaacatt tttttcatca taatttcctt aatggttgaa accccgccat
     9121 tcggacatcc gtccttcgtg gcagcagcat cagattttat ttgaaagggg tgtaacccct
     9181 ttcaaattag ggcaacacat agggcggcgc tttatgtgtc gtcctgtgtg ttgaaacatc
     9241 attgcccgta tcctgaaata gaaggctgtg ttttaacaat ccgttgattg cttaagatga
     9301 attcacacta aaaaacagtt ctctatttca tctattacgg cgggttgttt tgtttataag
     9361 ggtggtaatc tacaaattgt tactaaatag caaggtgtga atgaaacggc agatataatc
     9421 aaaatcttct tgttggtcaa aatacggtaa cataatgctt tttaattttt cgcctttggg
     9481 tttacgtcca taagaaaaca gatagcggtt tgctgtaata catttagcgt aatacagttt
     9541 tacttctatt ggcatatcgg actgtatggg tgttaatacg gcgacatcac tatttgctac
     9601 aaattcacaa gttgaaacat agctataagt atggctacct tcgccattgg tagaagtgaa
     9661 aagcgtatgg catggaaaaa tatgtttttc atcaacgcta tcacgagcga tataacttcg
     9721 tagcgttctt gcttgcgtac tggcaggacg gatatagaca accgtgtctt tttgccgttg
     9781 ttcaaattct tttaatttgg ttgcggcaat gccgttttga atagtaaaaa tttcatcaag
     9841 tcgttttgat tgtcgaacca caggcaaatc aagcggttct tttaatttcg gttcggttac
     9901 acccgcagat ggatttaaat tcacgctttc aacccagctt gggatctcat ccaaagatgg
     9961 cacaaacagt tgtcgtaacg tgcggttagc ttccctaccg caagcagaaa aacggtaggc
    10021 attggcaaaa atagcagcac aataaaagag tttttgttgt tctgtcattt ctgttttggg
    10081 atttaaaaca gcaacatttt gagctgtata aaatggtttg ttttgtaaaa aagtagataa
    10141 aacagaacca cctaatgcaa cagttaataa tcctgctgga taaggttttg tatttttcaa
    10201 taaatccaca gatgctgtta caccattatt tttgcctgac ctgcctacaa agttgattgt
    10261 tggattgaag ctgctcattt tattcaaatc taattttgaa ccgtaactca catcaaaaat
    10321 ctcttgcaat tcaaccatta ttcatcgtct ccgaagtcgt tattgtcatc gtcttcatag
    10381 ctttcattat ccaaattttc agtcggccct acttccgctc ccagtagttg aaataaagca
    10441 aattcacgca cgacttgctc aaaatctgct cgggtaattt tggaataatc agtttccaaa
    10501 taggcttcgg caacccattc atcgttatca gttacaaatg ctgttaccga ttctcctgtg
    10561 tgaacttcgt tgtttaaata atgttccaac cagcgcgctt taatgccctg ccagcggtcg
    10621 tataaatcaa tgcgtcccaa gtgtttggtt ttttcaaaac cgtcatcacg ccagtagcca
    10681 aaccacgttt tgcgacaggc ttttttcgtg ctgatttctt cttgtgtgtc cgggtcaatc
    10741 acgactgtct gaaaatgcgg tttatgggct tcaaatacga caatgcacgt taccgtgcca
    10801 actgggtaaa acagttcgct cggcatagac atcacggctt ttaagcgatg atatttcaca
    10861 atttcgcttt tggctttgct tggtgcaatc acgcaactga ctggaataat ggcaatgcct
    10921 gtgccacctt ctgccaacat atccaacatt tctttgacaa aataaagttc atgcaattcg
    10981 gcatcgcttt tggattgggc gtaaggtgga ttgagaaagc caacatttgg gcgttttaag
    11041 cctgtttcag gtttgggatt tttgattaaa tcctgaaaat cagtcatgaa acaactgctt
    11101 tggtgtaaat tggctttacc gtctccgcgt aaaatcatat tactggcggc aagagcaaac
    11161 atttttgggt tgttttcaat gccaatcagt tgatattttt tagagatggc ttacccaaat
    11221 tgctaattct agcaacagat ttaccgtcta aattgatacc acaaggaaca caaacttgtt
    11281 ctttggaaat gccacgtttc gttgctctcg ttcctcgttt gtgagcagga cgtggcagat
    11341 taaaattttt gtggtgccct ttgtaagaaa tcgtcgaata agtttcatca gcttgcacaa
    11401 tgccgtctaa ctcaacttca ttcatcatgt tttgaagtgc gtccaaaatt ttgtgccgcc
    11461 aagtaaacgc agtagcaaga ttgattttgc aaatttcagc acatttgcga agtgggtatt
    11521 tttcaatcat acaatgaatg tatttttccc aaacttcaat gtctttttgc gtgttgtaga
    11581 gaatagtgcc tgtctgctca acaaaagact ttttacaatc acgacacaaa aaacgttggt
    11641 tgccacaatc tttgccattt ttgacgaaat gtgtagattg gcaatgtggg cacttcgtaa
    11701 cttttcttgg ttcaatgact tttttaacct gttcttttga gctgacagaa gtcaaaaaag
    11761 cctgtttatc cgtatcggac aaattttcaa aaagttgttt taaaatgtct aattcttttg
    11821 ttacgcccga cattataaaa tcccttatag ttcaatttgt tataagggat tttatcaaaa
    11881 attagtgcaa aaatcaacgg attgttaaaa cacagccaaa tagaaaagaa aatgcccgtt
    11941 ttacggtctt ctttccgaca aaatgccgtc tgaaacgaat ttcagacggc atggtcgaaa
    12001 attattgcgc ggcaagcggt ttgagcagga gggtttcgcc gctttgcacg acattttcca
    12061 gcaaatcgat gttcggatgc ttccccggat gggcgcgcgc gtcggcaaca tgttcggcaa
    12121 accatatcaa accctgttct gccgcttctt tacccaattt tccatcaaaa cgttccgcca
    12181 aggcattgta cagcttgagc gaaccgagct tgccctgaac ggcgggaatg tggtgaaccg
    12241 ttttgccttc ggcatcctga acatccaaac cgcccagatg gtcgattgaa ggaaatgtgg
    12301 cgagatagtc ttggaaattc atatcaaatc cgaaaatcag tcagttagcg cgtgccgaaa
    12361 atcttgtcgc ccgcatcgcc cagaccggga ataatatagc cgttttcgtt caaacggcta
    12421 tccagcgcgg cggtataaat cgtaacatcg ggatgcgcat cattaacagc cttcacgccc
    12481 tcaggggcgg caaccaagac taaggctttg atgttgcggc aacctttttc tttcaacagg
    12541 tcgatggtgg ccaccatcga accgcctgtc gccagcatag gatcgataat caaagccgga
    12601 cgctcgtcca tactgtccac aaatttctca aaataggaaa taggcttcag cgtttcctcg
    12661 tcgcgctgca aaccgaccac gctgattttg gcagtcggaa tcaggtcgag cacgccgtcg
    12721 agcataccca aaccggcgcg caggatgggg acgacggtca gggttttgcc tttgatgcgg
    12781 tcgccttcaa tctgtccgca ccatccgtcg ataaggtatt tttcaatttc aaaatcacgg
    12841 cttgcctcgt atgccatcag gcgcgccaac tcggtggtca gcgtacggaa tttgtaggtg
    12901 ctgcaatccg cctccctcat cagggtcagt ttgtggcgga cgagcggatg gttgataacg
    12961 ttaacgttca tcgcggtgtt ccagtgtgat tttgagacgc gtcattatac ttttttttaa
    13021 tttgccggaa aagaaaatgc cgtctgaaac acgttcagac ggcattgccg aatccatcag
    13081 gctttcagca gctcttgcag ctcgcctgcc tcatacattt ccatcaggat gtccgaaccg
    13141 ccgacaaact cgccgttcac ataaagttgg gggatggtcg gccagtcgct gtattcctta
    13201 atgccttggc gtacttcggg attttccaat acgttgacag caacgtaatc ggtgcagcct
    13261 gccgcgttca ggatttgcac ggcgcgggaa gagaaaccgc attgcggaaa ctgcttcgta
    13321 cctttcataa acaatacgac gcggtgtgtc gttactactt ctttaatttg gtcgtggatg
    13381 gaagccatcg cttattcctt ttgataaagt tgacaaaatt tgtcaggtag ggcgcattat
    13441 acgcaaaagc ggaaacttcc cacaatattt gccgtatatc gcgcagtatc aatgttgcga
    13501 acggcaatat tcccactcct cgacaatcct gctgtccggc aaatggcaca gggcatatcc
    13561 gccgccgctt tcgtcttttt ttacttcccg tctgccgcct ttggcaatgc agaatttgga
    13621 agcggaattt gccatgcctg tccgaaacgt gttggcaggc agccggcagc agcgcggaca
    13681 tgcatacgga tgaattgcct tcatggtttt ccttatcgtc ggcacagggt cagacgaccc
    13741 gcatatcggg tactttttta aacagccggt aaaagttttc cgtcgtatat gccgcaacct
    13801 gttccaatgt ttgattccgc aatttggcaa tatgttcggc agtatgacgc acaaaagccg
    13861 gttcgttctg cctgcctcgt ttgggaacgg gggcaaggaa cggcgcatcg gtttccacta
    13921 aaatgcggtc gtccggaacg tatttcgccg cctcctgaac caagggggcg tttttaaagg
    13981 taacgattcc cgagaaagaa atataaagcc ccaaatccat ggcagcccgg gcaaaaccga
    14041 catcctcgga aaaacagtga atcacacccg aattggtttt acattctttt aaaatcgcca
    14101 aggtgtccgc cgccgcatcg cgcgtatgga cgataacggg cagtccggtt tgattggctg
    14161 cttcgatgtg gtctgcaaag cgtttgtgct gccaagacaa atcgcctttg caccagtaat
    14221 aatccaaacc cgtctcgccg atgccgacca ctttcggatg ggcggcggct tcggccattt
    14281 ccgcaatgga aaattcttcg gcttccttgc tgtcgggatg tacgcctatg gtgcaataga
    14341 tgtgttcgtg cgcttcggcg acggcaaaga cttcggagaa gctttcccta ctgacgctga
    14401 tggcgagtgc ctgccccacg ccgtttgctt ccatgttgga caaaacttcg ggcaggcgtt
    14461 ctttcaaacc ttcaaaattg aggtggcagt gcgaatcgat gatatgcata gcgttacatg
    14521 gtaaacgtag gtctgctgcc gctgattgtg ctgcccagtt cgacttcgat ctggtctttg
    14581 actttcaggc agttttcgtg ttttgtgaat gccagcccca cccctttggg tttggaggaa
    14641 gtactcgcag gattaatcca ggcgaccttg gtcggcagga acagtttggg gaagttgagg
    14701 atttctacgg caagcagaat atcgtccccg atggaaaata cgtcgtcggt ctgcacaaac
    14761 agaccgccgt gttccaaaaa cggcatatag gagctgtaca gcagattcat gtctttcagc
    14821 tgcaacgaca tcatttttgc cggaatattt tgtccgtctg acataattca cctgttctct
    14881 ttcttcaatt ccaaatagtt gatgagcaga tgctcgatct gcattttgac atttaaagta
    14941 tgaaacccgt aaggggcaag ctgtttgagc atatcctccg ccgcaaatac attgcgcgga
    15001 cggaaacccg atgccgtctg aagcagcctg tcttcataag cgggataata gacgggtttc
    15061 atgtgttgca ggcacaagcc taaatcgacc agccatttct gcatccaccc gacaaatacg
    15121 gcgagcggaa gtttttcctt atcgaaaagc gcggcgtaat ccaaaatctt caacaatctc
    15181 ggctctgcca atatctccag cagctttgcc ctcaattccc gaagctcgcc ttcctcctga
    15241 aacagcggcg cacccgaatg gaaagccaga cgttcctcag gctccgccac acccctgtcg
    15301 cgcagatacg ccaatgcttc cccatgcgaa ggagcgggca aaaccttctt ccggcagcgg
    15361 cttttaatgg tcggtaaaac cttgtccgcc gcgtggctta ccagcaaaaa gaccacttgt
    15421 ggcggcggct cttccaacac tttcaacaaa ctgttggcgg cttggacatt catactttcc
    15481 gcaggatgaa tcaggatcac gcgcaaaccg ccccgtaccg aagtcaggta cacattatcg
    15541 atgatttccc tgacggcatc gattttgatc cgcaacagtt tgcgtccgtt ttcgggttcg
    15601 tccgccagag gcgtgatttc gtaaaaatcg ggatggcttc cccgtccaaa cagatggcag
    15661 gacatacatt cgccacaggg tttgcagccc ggtgcagggg tttcgcacaa cagcgccttc
    15721 gccgcaaagc gggcaaacgc agtcttcccc gtcccctttt tgccaacaaa cagccacgca
    15781 ttaggacggc tcgtccaatg ctcggcaatc tgccgccatt gttcctgatg ccacggataa
    15841 atcatgtcaa actgcccgtc cggaaatcca taccgccatc agagcagttc caaatcgggg
    15901 tcggaacttt gtgccctgcg gctgcgcaac tgtaccgcca aacgcaaatc atgtgcggaa
    15961 tcggcatttt tcaaggcatc ctgcaaggaa atctcgcctt tttcatacaa ttggtaaagg
    16021 tgttggtcga aggtctgcat acccagggta gtggattttt tcatcacttc tttgatttca
    16081 tggatgttgc cgttgtgaat caactccgaa atcaggggcg aattgagcag cacctcgact
    16141 gccgccaccc tgcccttgcc gccgtctcgc ggaacgaggc gttgcgaaat aaacgcctga
    16201 aggttgagcg acaaatccgt cagcaattgt tcgcgccgct cctcggggaa gaagttgatg
    16261 atgcggtcga gcgcctgatt ggtgctgttg gcgtgcagcg tcgccataca caaatgcccc
    16321 gtttcggcaa aggcgatggc gtagtccatt gtttcacggt cgcggatttc gccgataagg
    16381 atcacatccg gcgcctgacg cagcgtattt ttcaacgccg ccatccagtt ttccgtgtcc
    16441 acgccgacct cgcgctgggt aatgatgcag tttttgtgtt cgtggacaaa ctcgatcgga
    16501 tcttcgatgg tgatgatgtg tccgaacgaa ttttcattgc ggtagtcgat aagcgaggcg
    16561 agcgaagtcg atttgcccga gccggtgccg ccgacaaaaa taaccagccc gcgttttttc
    16621 agcgcaacat ccttcaaggc cggcggcagg ttcaggcttt caaacttggg aatcttgctg
    16681 gtaatcgcgc ggaataccaa cgccgtcgca ccgcgctgta tcatcgcatt gacgcggaag
    16741 cggctggtgt ccggcaggct gatggcgaag ttgcactcgt tggtcgatga aaattcttcc
    16801 gcctgcttcg cactcataat cgaaaaggcg atttccatac atttttccgc cgtcagcggt
    16861 tcgtccgtga tgcgggtgat tttgccgtcc agcttcatag cgggcgggaa atgggtcgtc
    16921 acaaacaggt cggaaccttt gtttttgttc atatggcgca gccatgcgaa cagttcttcc
    16981 tttgcagtca tattttcatc ggtaaacatc ctcgtccccc aatcttcaaa caatctgtac
    17041 cttcccattc taccaaaaat ccgccgtgct gtctgaaacc gtttcagacg gcacggacaa
    17101 accgcctcca ctcaatcctg ccgtgttagc gtgtataatt cggcgttcta tccgaaattc
    17161 acagcaggtt agcaatatgt ctttgaaaac agtagccgtt atcggcgcga tggaacagga
    17221 aatcgagctt ttgcgcgaga tgatggaaaa tgtcaaagcc gtctctttcg gcagattttc
    17281 cgcctatgaa ggcgaattgg cgggaaaacg catggtgctt gcattgagcg gcatcggcaa
    17341 ggtcaacgcg gcggttgcaa cggcttggct tatccgtcaa ttcgcaccgg actgcgtcat
    17401 caacaccggc agcgcaggcg gtttgggcaa gggtttgaaa gtcggcgacg tggtgattgg
    17461 cacggaaacc gagcaccacg atgtcgatgt aaccgcattc ggctatgccc gggggcaagt
    17521 gccgcaactg ccggcaaggt tcgcttcaga cggcatattg attgaaacgg caaaacgggc
    17581 ggcgcggacg tttgaaggcg cggaggtaga acaaggcctg attgtcagcg gcgaccgctt
    17641 tgtccacagc agcgaaggcg tggcggaaat ccgcaagcac ttccccgaag tcaaagcagt
    17701 ggaaatggaa gcggcggcga ttgcccaaac ctgtcatcag ttggaaacgc cgttcgtcat
    17761 catccgcgct gtttccgatt cggcggacga aaaggcagac atcagctttg aagagttttt
    17821 gaaaacggcg gcggcaagtt ctgcaaaaat ggtggcagaa atcgtcaaat ctttataaaa
    17881 cctttatcaa atcatgccaa aacagcggtt ttccgttaga atatcgggct atttccacgt
    17941 tgcgcccgat attcggacgt accgctgttt gttcagacgg catttctaaa acctaccgtt
    18001 cagcattaaa aacaactcaa tgaaaaatat ccgaaatttc tccatcatcg cccacatcga
    18061 ccacggcaaa tcgacgcttg ccgaccgctt catccaatat tgcggcggtt tggatttgcg
    18121 cgaaatgagt acgcaggtgc tcgattccat ggacatcgaa aaagagcgcg gcatcaccat
    18181 caaagcgcaa accgccgcac tcaactacaa agcacgcgac gggcaggtgt atcagcttaa
    18241 tttgattgac acgccgggac acgtcgactt ctcttacgaa gtttcccgtt cgctgtcggc
    18301 ttgcgaaggc gcgcttttgg tcgttgacgc gtcgcagggc gtggaagcgc aaaccgtggc
    18361 gaactgctat accgcgattg atttgggcgt ggaagtcgtg cccgttttga acaaaatcga
    18421 cctgcccgcc gccgaccccg agcgtgtgga acaggaaatc gaagacatca tcggcatcga
    18481 tgccgtcggc gcggtgcagt gttcagccaa aagcggcatc ggcgtggaag acgttttgga
    18541 agaaatcgtt gccaaaattc ctgcgccgac cggcgacgaa aacgcgccgc tgcaagccgt
    18601 tatcgtcgat tcgtggtttg acaattacgt cggcgtggtc atgctgattc gagtgaaaaa
    18661 cggcaccatc aaactgaaag acaaagtgcg ctttatgagc accaaggcgg aaacgcaggt
    18721 cgagcagctg ggcgtattca caccgaaatc ggttcaaaaa caagaactaa aagccggcga
    18781 agtgggtttt ttgattaccg gcgtgaaaga attgggacag gcgaaagtcg gcgatacggt
    18841 tactttggtt gccaaccctg ccactgagcc gctgcccggt ttccaagaag tacaaagcca
    18901 agtatttgcg ggtctctatc ctgtggaaag ccacgattac gaagccttgc gcgacgcttt
    18961 ggaaaaattg cagctgaacg atgcttcgtt gaaattcgag cctgaagttt ctcaagcatt
    19021 gggcttcggc ttccgctgcg gcttcttagg cctgctgcat ttagaaatcg tgcaggaacg
    19081 cttggaacgc gagttcgaca tggatttgat taccaccgcg ccgacggtgg tttacgaagt
    19141 cgtgttgaaa agcggcgaaa aaatcgaagt tgaaaacccg tccaaactgc ccgacatcgg
    19201 cagcatcgaa accattctcg agccgattat taccgcgacc atccttgtgc cgcaggaata
    19261 cgtcggcaac gtcatgactt tgtgtaacca aaagcgcggc gtgcaggtca atatgcagta
    19321 tatgggccgc caagtgatgc tgacttacga cttgccgatg aacgaagtcg taatggactt
    19381 tttcgacaaa ctcaaatcca cttcgcgcgg ttatgcttcg ttggactacc atttcaaaga
    19441 attccaaccg tctgatttga ttaagctcga cattatggtt aacggcgaaa aagtcgatgc
    19501 cttaagcctg attgtacacc gtcaaagcgc ggttcacaaa ggccgcgagc tggcatcgaa
    19561 aatgcgcgaa ctgattccgc gccaaatgtt cgacatcgcc gtccaagccg ccatcggcag
    19621 ccggattatc gcccgagaaa acgttaaagc cctgcgtaaa aacgttttgg cgaaatgtta
    19681 cggcggcgat attacgcgta agaaaaaact gcttgaaaaa caaaaagcag gcaaacgccg
    19741 tatgaaacaa gtgggcaacg tggaaattcc gcaaagcgcg ttcttggcga ttttgcaggt
    19801 gagcgacaaa taatatttcg gcacataggg cgggcgcgtc gggaacacct ctcaaaccgc
    19861 cctgatttca gcggcacgcg cccgcaaaca aattttgcaa tttgccgcct taaaacggtt
    19921 gcacggccgg gcgggttttt aaccgttaag gaacaacaat gaacacaatg ctaatgtcgg
    19981 gcgcggctgc cgcgctgctt gccggcatca tcctttattt caaaagcgac aagaaacggc
    20041 aggaaaacgg tgaatggagt tccggcctcg aatacgccta tatcctgacg gcggtcggtg
    20101 tgtttgccgc tttgtccctg tttatgagct ttaccgccgt tttcctgatt ttcgttgtat
    20161 tgtgcggtac ggcttggggg gtatataaat accgcctgaa gactcatccc gaaatctcgg
    20221 aaagcagcca cttcggcgat tatttcggca gtttcttccc taccgttttg gtattgttcc
    20281 tcatccggtc gtttattgcc gaaccgttcc aaatcccgtc cagctcgatg cgcccgggat
    20341 taatcaaggg cgatttcatt ttggtcggca aattttccta cggcctgcgc gtacccgttt
    20401 taaacaatgt attcatcccc acgggcaaaa tcgaacgggg cgatgtcgtt gtttttaact
    20461 atcctttgca gccggagatg acctacatca agcgtattgt cggcatcccg ggcgacattg
    20521 tcgaatatcg agataaagtt ttgacggtaa acggaaaacc ggcttccgac attcctgacg
    20581 gcacataccg ttatcctgac gataccgacc catccgaaat ccacaacacg gatatgttcc
    20641 gcagcggtct ggacggcaaa tccttcaata ttctgaaaaa agaaggacag cctgccgttt
    20701 ccctgcccgt attgggcaaa tatacctccg atattatgtc tgaaaacgga tattccatag
    20761 atcaaagcgg tctgaaccat tgccaatatg ccgacgacgg cagcggtttc gtgtgcaaag
    20821 ttcccgaagg acgctatttc gctatgggcg acaaccgcga caacagtgcc gattcgcgct
    20881 actggggctt tgtggatgac aagctggttg tcggcaaggc aatgttcatt ttgatgaact
    20941 tcggcgattt cggcagggcc ggcacggcaa tccgttaagc cggaaaatta cgccggaaca
    21001 tcaaaaatgc cgtccgaacc ctgtttcaga cggcattttt atcatcaaac tccttgtttc
    21061 cctaccggca gcctcgggga taatatagtg gattaaatct aaaccagtac agcctggcct
    21121 cgccttgccg tactatttgt actgtccgcg gcttcgtcgc cttggcctga tttgaattta
    21181 atccactata tgcaccgcct tgccggcatt atcgatatgg gcggcacata tggattgcct
    21241 ggaaaaatga tgcgttcggg cggcgtatga tttgcctgtg tttaaatcat cggttgattt
    21301 tatccgtcgg caagtgaaaa gccggttatt tccgtttgtg tggaaaccca tcccattgaa
    21361 taaagggcat ttcgtccgac tgacggtgtg ggcggcatat ggaaatacgg acattatttt
    21421 agttgcattg ccattcctgc ggaggcggct tttttgattc tgcccgcccc gataccgtct
    21481 gaaagggctt cagacggcat tttccgctcc gcgccgttgt cggcatgttt ggtcttgccg
    21541 gttttgactg cctgccagtc caatcgtacg caggtttgcc acggcagaaa atctgatgcc
    21601 gaccggagtc aaagctcgga cggctgtttc agcgaaccga ggtaaatcgc cgaaagcgtc
    21661 agcaccaccc ctgcccactg cacgccttca atcgttttgc ccaatccgaa ataatcgatg
    21721 aacagggcgg cgaccggttc ggacaaaagc aacagccccg tcagcgacag ggaaagcagc
    21781 ggaatcgcat aggcaaccat cgcccacgca aaacactgca tcaccacgcc ccgtacacaa
    21841 gcaccaaacc cgaatctttc caagtggtcg gataaagtgc gccgccgtcc atcagcaatg
    21901 ccggaacaat cggcgatgcc gcgccgccca aactcaaaat catcattgaa gggaaaagcg
    21961 ccacctgttc gacttcatga gtcttgcgga cgaacaccat agatagggca agcatcagcc
    22021 cggacaccaa accgctggcg aatccccaaa ccgcattacc gttgtagccg aattccgcac
    22081 cggcaatcat cgccacgccc acaaccgccg acatcaagct tgccgcctgc agcctgccca
    22141 aacgctcgcc gaaaaagaaa acaccgattg ccgacaagaa aaagatttgc aggctgttga
    22201 gcagggtgga aatacccggc ccgaccgcgt gtatgctttc gtgccaaagt gccaaatcga
    22261 aagcgagaaa cacgcccgcc gtcagggcat atcggacggt ttttctgttt tttgggaatt
    22321 tttgtccgaa aaaccgtgcc aaaaaccaaa ataccaatac cgaaatcagc aaccgccaaa
    22381 atgcgattgc atacgggccg acggggacgg atctgacaat caggctgccc aatccgaaca
    22441 ccgcgcaacc cagcaccagc aatggtgcgg catatttttt tccgttaccg accacaatat
    22501 cccctccctt ccccatatgg ctgccgcgcc gccggcaaaa gtgccgccat tccgaaaata
    22561 tgccgtctga agcccaaagc cccggctgcg cccggacaaa cggctgactt tacgggcagg
    22621 ccggctattt tccgttgggg caaaatccgt cctgccggat aaaacccgaa taaaaaatat
    22681 ttccctcaag caatgcggcg gcgccaacac ctgattaaaa cacaaccggc gttttcggta
    22741 tatatgcaaa aacgcatttt ccggcacggc gttacaccga taccgaaaaa tgcgtctgaa
    22801 tgtttcgaca atccgattaa agcattatgc caaatctgca aaaagtggcg tagagagata
    22861 gcgttcgcca taagaaggca gcagcacgac tatcagcttg ccttcgtttt caggctgttt
    22921 ggcaagctgc aacgcgctcc aaaccgccgc accggaagaa atgcccgcca aaatgccttc
    22981 tttttccgcc attgcacggg cggtttcaaa agccgcttcg ttcggcactt tggcaatgct
    23041 gtcgtagatt ttggtattca aaacggtcgg aataaaacct gcgccgatac cttgaatcgg
    23101 gtgcggacct ttttcgccgc cgctcaatac gggggaagcc tcaggctcga cggcaaccac
    23161 tttaacttcg ggtttgtatt ttttcaatac ttcgcccacg ccggtaatcg taccgcccgt
    23221 gccgacgccg gcaacgaaga catcgacttt accgtcggta tcgttccaaa tttcctcggc
    23281 ggttgttttg cggtggactt cgggatttgc ctcattgtcg aactggcgcg gcataaaata
    23341 agtgtctgga tgagcgtcca ccaaggattg cgctttggca atcgcgcccg ccataccttc
    23401 ggcggcgggg gttagaatca gttccgcgcc aaacgtgcgc aacagcattt tgcgctcttt
    23461 gctcatgctt tccggcatgg taatcgccag tttgtagccg cgtgcggcac ataccattgc
    23521 caaaccgata cccgtattgc cgctggttgc ttcgacaatg acggtgtttt tgttgatttt
    23581 tcccgctttt tcggcggcct cgatcattgc ttcggcaatg cggtctttga cgctgctgcc
    23641 cggattaaaa aattccagtt tcacggcaac ctctgccttc aaacctttgg tcaaacggtt
    23701 cagtttgacc aaaggcgtgt tgccgatcaa ttcggtgatg ctgtttgcaa ttttcatctt
    23761 ctgctcctga ataataaggt ttcgacagta aggcgcaatc cccatcggta tcctgtccga
    23821 ttgcagggga tttgcgccga accataaaca aatttcggat atggctttat cctaaaccta
    23881 tgccgcctaa aaaaccaata cccaaaattt atttctttat aactttttgt tccgatacag
    23941 acggcatcac aatcatctgc cgatttcggc aagcagcatc gccgttaccg caacctgcat
    24001 caacatcgcc gccaacacgc cgagtcgcag ctgcgaaccg ccgaacgttg acggcggctg
    24061 ccggtctata ccgcagcggt ggacataggc aaccgccatc aatacaaact gcaacacgaa
    24121 ccaataaacc ggcttcagcg cataaagttt gagcaatgcc gcaaacacaa acacttgaac
    24181 cgttcccgaa aagcaataat gcaccaacgc acatattccg acaaaagcaa gcgtcatcga
    24241 tacattactg acggcaaaaa gcccgagcag cggatgttcg gggtctgcct gccatccgtt
    24301 tccatctgtt ttccggttcc aatacccgat gaaaaaaaat atgctgccca aagtcaggta
    24361 aaaatggggg atgaacaaag gcgcggcgcg ggtcattccc cacatccccg gcatcagctt
    24421 tacccctaaa accgagatgc cgagccacaa cgcaatactc gcccaaaacc attgcaacat
    24481 tcctgcgcgg aatatcagaa acagaaaaac cgccgcatac agtaaggcag acaaaaaatc
    24541 caaatgcagc aaaatcatga atacgccaac tcgccttcaa acacggtttc cgcagggccg
    24601 gtcatcatca catcgccgcc gcaggcccat tcgatatata aagtcccgcc cggcaaaacc
    24661 acctctaccg ttttcccttc atccaacagc cccagacgga tacccgccac cacagccgca
    24721 cacgcgcccg taccgcacgc ttgggtttcg cccacgccgc gctcgaacac gcgcaaacgg
    24781 attgccgtcc gtccgacaac ctgcataaag ccgacgttga cgcgttcggg aaactgcctg
    24841 tgcggttcga tacgcgaacc ggtttcgcgc accggcgcgc attccacgtc atcgactaca
    24901 atcacagcat gaggattgcc catattgacg cagctgacag gctgaatgcc ggattcgaga
    24961 tgcaccccgt aaatacaggc atcatccccc tcgcccgatt cggggacaaa cggtatttca
    25021 gacggcataa acttcggttt gcccatattg accgtaacca taccgttatc ggacaatttc
    25081 ggaaaaataa tgcccttggc cgtttcaaca caaatttctt tcttatcggt caaacccttg
    25141 tctgcaacaa aacgggcaaa acaacgcgct ccgttgccgc attgcccgac ctcgctaccg
    25201 tcggcattga aaatacggta acggaaatcc acgccttcgg tttccgaaca cccgaccacc
    25261 aaaagctggt cgaagcccac gccccggaag cggtccgccc atgccgcaat cggcacgtcc
    25321 tcgggggtaa aatcctgact gaccgcgtca atcaccataa aatcgttgcc caaaccgtgc
    25381 attttggtaa atttcaaagt tttcatattg ttccgaaatc ccgtattgtc tgttgcttcc
    25441 gaatccgaaa tccccgagcc cgaagggaaa tgcttccatc tcccgccgcc tgaggattaa
    25501 aatgagccat tatactgttt gttacccctt acaagaaaca caatgtcccg cctgttcctg
    25561 ctgcttttcc cttgggcaac ccttgccgcc tgcacccgaa acataccggc aagccccgcc
    25621 gtttcaaatt gcgcgcatct tcacaaccgc accgtcctgt attttgacgc acacggcaaa
    25681 cccgccgaca gcacagcaaa gggcggattt tacaggaccg tcgaaattca tgacacaaac
    25741 cgcatcctta ttcaagactt taacagcacg gggaatgccc tcgtcaaacc ctattggtcg
    25801 gactgccgga aattgtccgt tttccccgac aaacatctga ccatgagcca tccatgaacc
    25861 acgacatcac tttcctcacc ctgttcctgc tcggtttctt cggcggaact cactgcatcg
    25921 gtatgtgcgg cggattaagc agcgcgtttg cgctccaact ccccccgcat atcaaccgct
    25981 tttggctgat tctgctgctt aacacaggac ggataagcag ctatacggca atcggcctga
    26041 tgctcggatt aatcggacaa ctcggcattt cactcgacca aacccgcgtc ctgcaaaata
    26101 ttttatacac agcctccaac ctcctgctgc tctttttagg cttatacttg agcggtattt
    26161 cttccttggc ggcaaaaatc gagaaaatcg gcaaaccgat atggcgcaac ctgaacccga
    26221 tactcaaccg gctgctgccc ataaaatcca tacccgcctg ccttgctgtc ggaatattat
    26281 ggggctggct gccgtgcgga ctggtttaca gcgcatcact ttacgcgctg ggaagcggta
    26341 gtgcgacaac cggcggactg tatatgcttg cctttgcact gggtacgctg cccaatcttt
    26401 tggcaatcgg cattttttcc ctgcaactga aaaaaatcat gcaaaaccga tatatccgcc
    26461 tgtgtacagg attatccgta tcattatggg cattatggaa gcttgccgtc ctgtggctgt
    26521 aagccccatt cattcccgat acccgtaaaa aataccgtct gaaacctgtt cagacggtat
    26581 tttcacccta agcgttactc ggcggcattt tcttcacgga caggcgcatc cagcagggct
    26641 ttgatggaca gacggacacg acctctgtca tccacttcca atgctttcac gttcaccacc
    26701 tgaccgactt gcaggtaatc gccgacattg cgtacgcgct cgtgtgcgat ttggctgatg
    26761 tgtaccaaac cgtctttgcc cggcatcacg ctgacaatcg cgccgacgtt gttgtcgagg
    26821 attttaacca cagtaccttc gtacactttg cccacttcca cttcggcagt gatttcttcg
    26881 atgcgttttt tcgctgcatc gcccgcttct tgagtagtcg cagcgatagt gatggtaccg
    26941 tcttcagcga tattgatttc agtaccggtt tcagcggtaa tcgaacgaat ggtttcgccg
    27001 cctttaccaa tcacatcacg gattttgtct tggctgattt tcatggtgaa caaacgcggc
    27061 gcgtgtgcgg acagctcttg cggacccgca acggcagctt tcatctgatc caagatgtgc
    27121 aaacgcgctt ctttggcctg tgccaaagca atttgcataa tctctttggt aatgccttgg
    27181 attttgatgt ccatttgcag cgcggtaacg ccttcggtcg taccggccac tttaaagtcc
    27241 atatcgccca agtggtcttc gtcgcccaaa atgtcggtca ggacggcaaa cttattgcct
    27301 tccaaaatca gacccatcgc gataccggca acgtgtgctt tcaaaggcac gccggcagac
    27361 agcaggctca aacagccgcc gcagacagaa gccatagagg aagagccgtt ggattcggta
    27421 atttcggaga ccacgcgcat ggtgtagctg aaatcttcag gtttcggcaa tacggccaac
    27481 aacgcacgtt tagctaaacg gccgtgaccg atttcacggc gtttcggcgc gcccatgcgg
    27541 cccacttcgc cggtagagta cggcggaaag ttgtagtgca gcataaagcg gtcggtatat
    27601 tcgccggaca acgcgtcgat gatttgttcg tcgcgcgaag tacccaaagt cgctacggca
    27661 agggcttggg tttcgccacg ggtaaacagt gcggaaccgt gcgtgcgcgg caatacgccg
    27721 gtttggatgt tcagcggacg gacggtgcgg gtgtcgcggc cgtcgatgcg cggttgtccg
    27781 tccaaaattt ggctgcggac gacatcggct tccaagtgtt tgaaaatacc tttgatttcg
    27841 ttggctgcca gcgtgtcggt ttcttcggta atcaaggctt cttttaccgc attccaagcc
    27901 tcgtccaatt tggcggaacg cgcttgtttt tgacggattt tgaacgcttc tttaatggtt
    27961 tcgccggcaa tcccgcgtac tttggcaacc agttcttcat tggtttcagg tgctttccaa
    28021 tcccaaactt ccggattcac ttcgtcggca aattcattga ttgcattgat ggcaacctgc
    28081 atttgatcgt ggccgtaaac caccgcgccc aacatcacgt cttcgggcag gattttggct
    28141 tcggattcca ccatcaacac ggctttggaa gtaccggcga ccaccaagtc caattgtgat
    28201 ttcgccaact cggctttagt cgggttcaaa acgtacacgc cgtttatata acccacgcgc
    28261 gccgcgccaa tcgggccggc gaacggtacg ccgctcaaca ccagcgcggc ggacgcgccc
    28321 aacattgcag gaatgtcgga atcgatttcg ggatcgacgg acacaaccat cgctacgatt
    28381 tggatgtcgt ggtagaaacc ttcggggaac agcggacgaa tcgggcggtc gatcagacgg
    28441 ctggtcagga tttctttttc gctttgtttg ccttcgcgtt tgaagaagcc gccggggatt
    28501 ttgcctgcgg cataagtgcg ttccaaataa tcgacggcca acgggaagaa gtcctgacct
    28561 tctttgacct ctttgttggt ggtaacggca acaaaaacaa cagtgtcgcc catcgatact
    28621 ttaacggcag cggcagcttg gcgggcaatt tcgccggttt ccaaagtaac ggtttgatta
    28681 ccgtattgga aggtcttaac gtatttattg aacatcattg ttcctttcaa aataccgcac
    28741 tgctaaaaca ctaataatgc acactaaaat ccgaatgtgc atagttaggg tttcagaccg
    28801 tgcggcaggt tgtaaacaag ctttcagacg gcatttcgga cgctgaaaac aattgcggat
    28861 tataaaggca accatcctta aaatccagta ttgatacaat aaaaaggccg tctgaaacat
    28921 atccttttca gacggccttc agtcttcaaa atcaatcttg tgtcagtaaa gtcaatgctt
    28981 cctgatattt ctcgaccgtt ttccgaatta catcggcagg cactttaggc gcaggggctt
    29041 ttttgttcca accgctttgt tccagccagt cgcggacgaa ttgtttgtca aaagacggcg
    29101 gattggtgcc gacttcatat tgatccgccg gccagaagcg gctcgaatcg ggagtcaata
    29161 cctcatccat cagcgtcagc gtaccttttt catccaaacc gaattcaaat ttggtatcgc
    29221 aaatgatgat gccgcgcgat ttggcatatt ccgccgcttc ggtgtaaagc cgaaccgctt
    29281 tgtcgcgcac ttcttccgcc aattctttac cgataatgcg tccgcattct tcaaagctga
    29341 tgttttcatc gtgatcgccg actgcggctt tagttgaggg cgtaaaaatc acttcgggta
    29401 gttgttgcgc ttcccgcata ccttcgggca gccgaatacc gcaaaccgag ccggtttttt
    29461 gataatcttt ccaaccgctg cctgccagat aaccgcgcac aatggcctcc actttcaccg
    29521 gagtgagttt tttagctaca acggcgcgtt tttccaaagc tttggcttcg ttttcaggca
    29581 aaacatcgta cacggtatcg ccggtgaaat ggttgggcat aatatgcgcc agttttttaa
    29641 accaaaaatt ggaaatctgc gtcaggattt cgcccttacc cggaatcggg gcgtccaaaa
    29701 tcacatcaaa cgcggacagg cggtcggaag cgaccatcag catacgttta tcgtcgattt
    29761 catataaatc gcgcactttt ccggaataaa tctttaccaa accaatctca ctcatttcgc
    29821 acccccctga aaatatcttg aaaataccga cccgacaggt ttgaatcgca aaccgatatt
    29881 ctagccgaag tcggcgcaaa acaataccca tggcacaaaa agccaacccg tcaaccgtcg
    29941 gcaaagtttt ggcactataa taccgacagc aagtcctaca atacactttc accaaaggaa
    30001 atacctcatg agaatcctat tgacaggctc gaaaagccaa ctggcacact gcctgcgcga
    30061 ccgtcttcct gaagactggg aaaccattgc gacggattcc gcatccctag acattaccga
    30121 tgccgatgcc gtctgcaaca tggtcaaaag tttccaaccc gacgctattg tcaacacggc
    30181 tgcctatact gccgtcgaca aagcggaagg cgatgcggca gcggcatttg ccgtcaatgc
    30241 ttccgccgtt tacaaccttg ccttggcagc acatcgcgcc catgcccgat tcatccacat
    30301 ctcaaccgac tatgtctttg acggaaaagg gaaaataccc tatcaggaaa gcgactttac
    30361 caacccttcc aatgtatacg gacaatccaa aaccgccggc gaactgctcg cactgtctgc
    30421 caatcccgac agccttatcc tgcggacttc ttggctgttt agcgaatacg gggacaactt
    30481 tgtccgcacg atgctgaacc ttgcgcggga acgttccccg ctgtccgccg tccacaacca
    30541 aatcggctgc ccgacctatg ccggcgactt gtccgccgcc atcatccgcc tgttgcagca
    30601 gtccaatccc gttcgcggca tttaccacta cgccggcggc aaatccgtat cctggtacga
    30661 atttgcccgg cacatctttc agacggcatt gcaacaggat gcctcttttc ccgttcccga
    30721 actgaaagcc gtttcggatg aaggctctgc ggctgccgcc cccaggcccg catacagcat
    30781 tttggactgc cgcaaaatcg aaaacgactt cggcatcaaa ccgtcagact ggcaaaaagc
    30841 ccttgcacag gtcgtttcca agctgctctg atgccgcccg cccctccgtt tcctccgcca
    30901 agcaccgcct tggcggtttt cttataaaat ctactgatta cacaaacaca tatcatcttt
    30961 acacaatcat gcttccatca acagcaaaac atgatatgat tgccaacaat gacatctgac
    31021 aataaatttt ctaattttta ttgaaaaaat caataaatta agaattctcc caaccgacaa
    31081 ataaaataaa gaaagggtca atatgcaaca ccgtagaaga ttggcaattt accaggcatc
    31141 caaacgtgct tcctttaccg gcaggtcatc agccccacaa aaacgtaaag aacgttgatt
    31201 tgaaaaaaat gccgtctgaa gtcctgcttc agacggcatt ttttaccgtt cgagaaactg
    31261 tttcaacctg tcctcatcca aaaaccagtg gcaaatttcc tcatctcccg ccaacaaaac
    31321 gggaatcagc tcattgtatt tttcttccaa aacaggattt tcatccacat cgaccacttc
    31381 cagcccgaac ccgtattcat cctgaaaagg tttgagtgca tcgcgcattt tgtggcacaa
    31441 gctgcaatat tcacgaaaca tcaaggtcaa tttcatccgc gtttccttat ctgtcaattt
    31501 gcacacgcca aagccttaga cgcagcagaa tcatggtcta tttgggaaaa aacaatgtct
    31561 tcgaggaaga tgatactcaa gtcctgccaa aaacagcaaa aatgccgtct gaacagttca
    31621 gacggcattt ccaaaaccgt tttacgcttg aacgttgata cccgccgtaa ccgtcggttg
    31681 cgcattgtgc gccgtcagaa acgaagcggc aagctccccg cagcagccgc agcagctcgc
    31741 cacgcccaat tgcgactgca aatcgcccat tgtggtcgcg ccggcagcga tggtttcctt
    31801 gatttgatgg tcggtaacgg cattgcagat gcagacaaac attttgtgct ccgtgtgttc
    31861 tcaaactatc gtaccgatag cggcttttat tatcgtatgc aaatataaat aaaaacggtt
    31921 cgcattgcaa ggtcggcata cacggtttgt cttggtaatt ttttatcaag tttgcatttt
    31981 cgattgattt cttattaaac cgaagcagaa gcctcagttt cgggaatggt gttccttaac
    32041 cacgctatgc agccgcacat tggcaacatg ggtataaatc tgagtcgtgt tcaaatcggc
    32101 gtgtcccaac atatcctgaa ctacgcgcaa atccaatccg tgccgcacca gatgcgtggc
    32161 aaaggcgtgg cgcaggctgt gcgggctgat gtgcccgatg cctgccctgc cggcatattc
    32221 tttgacaatc atccacgcca actgccggga aatgcccgtt tttttctgac tgacaaacaa
    32281 tgcgtcgcaa ctcctgcctt tcagcagaag cgggcgtgcc tccgtataat agcgttccac
    32341 ccaatacgct gactcctgtc ccatcgggac catcctctgc ttgtcgccct tgcccagcgt
    32401 ggtaatacaa cccctgtcca aatccacatt gccgaaattc agcccgaccg cctcgctgac
    32461 gcgcaagccg gtcgcgtaca tcaattcgag cagggcctta tcccgcaaac cgtgcggcgt
    32521 gtcggtatcc ggtgcggcaa gcagtcggga aatctgctgt tctgtaatca gtgtcgggat
    32581 gtttctgtcg atttttggcg gtttcagcag gcgggtggga ttgtccgccc tcatcccttc
    32641 ccgttccatc cacacataca ggcgtttgca cgccgacagt gcgcgtgcct gcgaactccg
    32701 ttgctccccg tcaacataaa ccgccgccgc caaatctgct tcgtccgcat ccttcagcgt
    32761 tctgccgcac aaagacagcc ggcgggcgat cttttccaaa tcccgccggt agctgtccaa
    32821 agtattccga ctgagccgcc gatccaacca caaagtttcc agcagcctgt caatcaaacc
    32881 gttttccata acgttccaaa ccaatgccgt ctgaatcttc ttcagacggc atggttcaca
    32941 tcatcgggaa agcgtttcca acacttcctg tgcatgaccc gccactttga ctttccgcca
    33001 ctcccggaca atttcgccgt ccttgttcag aacgaacgta ctgcgctcga tgcctaacga
    33061 ctctttccca tacagttttt tcaatttgat gacatcaaac aggcggcaca ccgtttcatc
    33121 cttgtcgctc aacagctcga accggaaacc ctgcttggcg caaaaattct gatgcgcctt
    33181 tacgccgtcg cgggaaatac cgaccacggt ataacccaat gcctcaaact gttccaaacg
    33241 cgcattaaaa tccaagcctt ccgtcgtaca gcccggcgta ctgtctttcg gataaaaata
    33301 cacgaccaaa ggcagatgtt ctgccgaatg aaaatccgca ccgctgctcg aaggcagggt
    33361 aaattcatat ttcatatcca tagtcctact cccgatattc ccattattca aaacggcacg
    33421 cagacgcacc gccgcaattg ccaaaccaac cccgattcta ccgccccaaa ggacaaggat
    33481 tcaaccgccg gaaacatcca aaccgacaca cgacggcatg aaaaatatcc atgtcaaacc
    33541 acaaaatatg ttccgattta aaaacagaat gttataaaac ccaactccaa aaacacaaca
    33601 agaccgcccg cctacgggca gtctcctgtc agacgacata ctttacagat ggctgttttt
    33661 tcaacaaaat aacgccaata ctcaaaaata cggaaccaaa aatgtccatc cgcaccctga
    33721 aacgcctgcc ctcatcgctg ctgctcggcc tctgcctttc cctgccgtca gcccaccttt
    33781 ttgccgacaa cgacatttta gggcaatttt tagaacagaa catgcttacc tcctccgatc
    33841 cgatagaaat attcgccgaa agcacgatat atcccgccga tacccaggcc attacgggcg
    33901 ggctgattct gtcctcccaa tccgcactgg tcgtcaacaa caaaacaggg caaatactgt
    33961 atcagaaaaa cgccgacagg attatgccca ttgcctccat ttccaagctc atgagcgcga
    34021 tggtcgtttt ggatgcaaac ttggacatga acgaaaccgt taccatcaca cccgacgaaa
    34081 tcgaccgcct caaagggacc ggcagccgtc ttgccatagg tacggcactt acacgtaaag
    34141 aactgctgca ccttagcctg atgagcagcg aaaaccgcgc cacccatgca ttgggcagaa
    34201 cctaccccgg cggcatgggc gcatttgtcg ccgccatgaa ccgcaaagcc caaagcctgg
    34261 gcatgtacgg cagccgcttt tacgaaccga ccggactcaa cttccaaaac gtttccaccg
    34321 ccaaagacct gagcctcatg gtcaacgccg ccgcccaata tccgcaaatc cgcaccaact
    34381 cgacttccaa ctacgcctcg gtacaaacca aaaacgggca gcaaaactat aaaaactcca
    34441 atgccctggt cagggaaggc atgtggaaca tcgaattgca gaaaaccggc tacatccgcg
    34501 aggcaggcag gtctatggtt gtcaaagcca acattcaaaa ccaacccgtt accatcgtat
    34561 tgctgaactc gcccacatcc gccacacgcg tcaacgacgc ccgcaaaatc gaatcgtgga
    34621 tgctgcagca acgctcctga catacaaatg cccggcggaa aaccgcccaa acacaaaaaa
    34681 attggtaatt tttcctattc caggctacaa ttcgacacgc acagggcatc tgcccgattt
    34741 cacaatcata atagcggagt taaaaaatat gacagctaaa ggacaaatgt tgcaagatcc
    34801 cttcctgaac gcattgcgta aagagcatgt tccggtttcg atttacttag ttaacggtat
    34861 caaattgcaa ggtcaggtcg aatctttcga ccaatacgtc gttcttctga gaaacacttc
    34921 cgtaacccaa atggtttaca aacacgccat ttccaccatc gtaccggctc gctccgtcaa
    34981 cctccaacat gaaaacaaac cccaagccgc accggcttcg acccttgtcc aagtggaaac
    35041 cgtccagcag cctgccgaat aatccgcacg aagcatgacg tgtcatatct ttcaatacct
    35101 taccggacaa cggtaaggta tttttatttt caggcggcat ttaaaaatat tattgaaaaa
    35161 catccttcca ttcgcccatt gccttttgcg ggaggcactt cccgaaggcg gcaatgcgct
    35221 ggacggcacc gccggcaacg gacacgacac ccttttcctc gcacaaaccg caggcaacag
    35281 ggggaaagtg tgggcattcg acatccagcc gcaagccctg aacaacaccc gatgccgtct
    35341 gcaggaagca ggttacagca atgtacggct catcttggac ggacatgaaa acctgaagca
    35401 atatattccg gagccgctgg atgcagccgt tttcaatttc ggctggttgc ccggaggcga
    35461 caagaatctg accaccgaca aggaaaccag cattcccgcc ctttgagcaa ccctctccct
    35521 gctgaaagaa gacggtatgc ttattgccgt cctctatccg ggacacgaaa acggcaaaca
    35581 ggaggcagaa gccgtcggac aatgggcaaa aaacctgcct caagaacagt ttgccgtttt
    35641 acgttacggc tttaccaacc ggaagaatag cccaccctat cttttggcat ttgaaaaact
    35701 gcgtcaaaaa taactgtttg cggtaaaata agccgattct aacccttatt ttgatacgag
    35761 ccgaccatgc agtatctgat tgtcaaatac agccatcaaa tcttcgttac catcaccatt
    35821 ttggtattca acatccgttt tttcctactt tggaaaaatc cagaaaagcc cttggtcggc
    35881 ttttggaaag cactgcccca cctcaacgac acgatgctgc tgtttacggg attgtggctg
    35941 atgaagatta cccatttctc cccgttcaac gcgccttggc tcggcacaaa aatcctgctc
    36001 ctgttcgcct acatcgcact gggcatggta atgatgcgcg cccgtccgcg ttcgaccaag
    36061 ttctacaccg tttacctgct cgctatgtgt tgcatcgcct gcatcgttta ccttgccaaa
    36121 accaaagtcc tgccattctg aaacactgcc atgaacaaca gacattttgc cgtcatcgcc
    36181 ttgggcagca accttgacaa ccccgcacaa caaatacgcg gcgcattaga cgcgctctcg
    36241 tcccatcctg acatccggct tgaacaggct tcctcactgt atatgaccgc acccgtcggt
    36301 tacgacaatc agcccgattt catcaatgcc gtctgcaccg tttccaccac cttggacggc
    36361 attgccctgc ttgccgaact caaccgtatc gaagccgatt tcggacgcga acgcagtttc
    36421 cgcaatgcac cgcgcacatt ggatttggac attatcgact ttgacggcat ctccagcgac
    36481 gacccccgcc ttaccctgcc gcatccgcgc gcgcacgaac gcagtttcgt catacgccct
    36541 ttggcagaaa tcctccctga ttttattttg ggaaaatacg gaaaggttgt cgaattgtca
    36601 aaacggctgg gcaatcaagg catccgtctt ttaccggaca ggtaattccg cacgcggatg
    36661 ccgtccgaac accggtttca gacggcattt ttcctttgcc gccaacacgc gtgcaaaaaa
    36721 aatcgcccct tgagaaaggg gcgcaaaagg aacacaaacc actaccaaaa ctttaaatct
    36781 gcaacactgc ctgccgcata ctgtacccga caggatataa ggccctcact aaatcgtttc
    36841 gagaaatcca aacttcttca tcaccgacag aaaatctgcc tttctccggc accagctcca
    36901 acaaaaacgg ctgaaccgcc gtatgcagct tgtccttaca ccgccccagc tttctggaca
    36961 acgcggacag ggcgcgtttg taggcattat ccttgcggtc gagctccctc gcactaccga
    37021 cccagctcaa acgaaggttg cggctttcat cttcaatcaa caaaatacct gtttcaactt
    37081 cccccacctc gggttcgcac gaaagcgcaa tataatattt gccgtcaata ttgacatatg
    37141 ccgcttcatg cacctgacag ttgcagccgg aagtggcaag caacgaataa acagaatcac
    37201 ggtgcagcct caaaatatcg gaggaaatct gtttgaaaat actttccggc atttttccct
    37261 cctttcgtct gctgcgttgt ttactggatt gactgcatta tacagacatc tcccaatttt
    37321 acaaatgata attgttccta ttttttaaaa agtttataaa ttattgataa atatatgaat
    37381 taatttttaa gatttaaaac ctttgtctca aaaaatacaa aaatgccgtc tgaaacgtgt
    37441 tcagacggca ttggcttcac aggcagtccg tttaaaattt cacgccctta tgcagcgcga
    37501 cgatgcccgc actcatattg tgataatcca cgctgtcgaa gcctgcatcc agcatcatct
    37561 gtttcaaagt ttcctgatcg gggtgcatac ggatggattc ggcgagatac tggtagctgt
    37621 ccgcatcttt ggcaatcagc ctgcccatta ccggcaacag cttgaaagaa taaaaatcgt
    37681 atgcgccttc gagcggcttg tagattttgg aaaactccaa caccagcaac gtaccgcccg
    37741 gtttcaaaac acggtacatt tccttaagcg cggcatcttt atacgtcatg ttccgcaagc
    37801 cgaacgccac ggagaccaag ttgaaataat tgtcggggaa aggcagtttt tccgcatcgg
    37861 caagcgacac gggtaaaatc atgccttcgt tcaacagacg gtcgcgcccg acggtcagca
    37921 tagaagaatt aatatcggtc agccaaacct cgccttcctt gccgacccgt ttcgcccaac
    37981 cgcgcgacaa atcgcccgta ccgcccgcaa tgtccaacac tttatcgcct tttttcaggt
    38041 gcgccgtatg gatggtaaaa tgcttccata cgcggtgcag ccctgccgac atcacatcat
    38101 tcataatgtc gtagtttttg gcgacggagt gaaacacttc cgccactttt ccggctttct
    38161 catcttcgtt gaccgtgctg aacccgaaat gcgttttttg tccgcccata ttctaaccac
    38221 ctccctgata ccgccctacg gcatcccaaa cttaacaatc tgattgcttt tcaaaacgcg
    38281 acgcgcccgc cgccgccaat ttgtccaaat attcctgcca catcgcatcg tatccgtatg
    38341 ccagtctgtg cagatacgcc caatcgtaaa gaccgctgtc gtgcccgtcc gaaaaactga
    38401 ttttcagcgc atactgtccg acaggctgca aatccgtaat ctgcacatcc gccttaccgg
    38461 tctgcaaaac atcctgtccc acgccgtgtc cgcgcacttc cgcactcggc gaatagacgc
    38521 gtaaaaattc ggcaggcaga tttttaggtt cgtccccata agtcaaaacc aacacggcac
    38581 ggtttttcaa caaacggatt tcaacaggaa tattattcaa aaaacacctc ggttcggcat
    38641 acaatatccg gcaggcgtac gcgtgccttt aaacggcgcg tattttaaca gctttatccc
    38701 gataccgata ttcaaaccca cacttcagac ggcattgata tggacaatct cgtatgggac
    38761 ggcattcccg acatccgcac actcgaccaa accatccgca aacacgcaca cccgctcaac
    38821 ctgattgtct gcctccccga taatcagatt cccgattttc aaaccgcaca agatgcttcg
    38881 gactcggaat gccgtctgaa gcaccgtttg gatcaggcaa cccagtgcct ccagttcgac
    38941 agcatcaacc tcatcgaaca catcctgccc gatgtccgct tctggctggt tcccccttca
    39001 cgcacccgcc gcctgcacga acacttccac cacatttcct ggcagaccga agccatcccg
    39061 caaaccgaaa gcaagtccga caaaccctgg tttgcacttc cacaaacatc cgaacggaaa
    39121 aaaccggaac acgtcctcgt catcggtgca ggcattgccg gcgcatcgac cgcccacgcc
    39181 ttagcatcac acggcatttc cgttaccgta ttggaagccc gaaaagccgc tcaagccgcc
    39241 agcggcaacc ggcaagggct gctttacgcc aaaatctcgc cgcacgacac cggacagacc
    39301 gaactgctgc ttgccggcta cggctacacc aaacgcctgc tcggacacat cctgcccgac
    39361 tccgacactt ggggcggcaa cggcatcatc cacctcaatt acagccgcac cgaacaacaa
    39421 cgcaatcacg aattgggttt gcaaaaacac cataaccacc tctaccgcag catcacgtct
    39481 gcagaagccg aaaaaatcgc cggcatcccg ctgaacacgc cctacgccga accattatgc
    39541 ggactctact ggcaacacgg cgtatggctc aatccgcccg cattcgtccg caccctcctc
    39601 agccatccgc tgatcgaact atatgaaaac acaacgttaa ccggcatttc ccacgacgga
    39661 gaaaagtgga ttgcaagcac gccaaacggc acatttaccg ccacacacat catctactgc
    39721 accggcgcgc acagcccctg cctgcccgaa accaacctcg ccgccctacc cctcaggcaa
    39781 atacgcggac aaaccggact cacaccgtcc accccgtttt ccgaacaact gcgttgcgcc
    39841 gtttcaggcg aaagctacat cagcccgtcg tggcacggac tgcactgcta cggcgcgagt
    39901 tttattccca acagcagcaa taccggatgg aacgaagccg aagaagcctc aaaccgccaa
    39961 gcattggcac accttaaccc cgcccttgcc gaatcattgt ttgccgccaa cccaaacccc
    40021 caaaaacacc aagggcacgc cgccatacgc tgcgacagcc ccgaccacct ccccgtcgtc
    40081 ggcgcactcg gcgacattgc cgccatgcgg cagacttacg ccaaacttgc actggacaaa
    40141 aactaccgca tcgacaccac atgcccatat ctgcccaatg cctacaccaa taccgcccac
    40201 ggcacacgcg ggcttgccac cgcccccatc tgcgccgccg ccgttgccgc cgaaatccta
    40261 ggcttgcccc atctcttttc acaacgcctg cgccacgccc tacaccccaa ccgcaccgtc
    40321 atccgcgcca tcgtcagaag gcagaacctg attccttaag cctttgccgc atgccgtccc
    40381 gaccacaaaa aatcatattc aaataaacat accaatcctg acggtaaaat atcgaaaaca
    40441 cccataacgt tttcttccca aaataaggag taaggaatgc ctaacaaatt gcctttgctg
    40501 tccaagatga attttccgta acattcgaag acttccttaa ccctgccagc cgcttcggtt
    40561 tctgtacccc ctgttagata aggaagggat gaaaaagccc tgttacttgt atgaagcaaa
    40621 gggcgttaat ttttgttaat cttcccttct caggaactca atatataagg atgttaattt
    40681 ttatttccct tctcgaggac tcaatag
//
LOCUS       DBJGGI010000009        40624 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.009, whole genome shotgun sequence.
ACCESSION   DBJGGI010000009 DBJGGI010000000
VERSION     DBJGGI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40624
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.009"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            137..517
                     /locus_tag="ACSIGJ_000509"
     CDS             137..517
                     /locus_tag="ACSIGJ_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HHQ1329466.1"
                     /translation="MPLKFQPRERSVIMCDFRGYEEPEMVKKRPVVVIARNRHNGKPV
                     TVVPLSSTEPVPLADCHHKMSENPLPDKPHIQCRAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAVKTAVAKAFKLY"
     gene            complement(718..1560)
                     /locus_tag="ACSIGJ_000510"
     CDS             complement(718..1560)
                     /locus_tag="ACSIGJ_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRO-N domain-containing protein"
                     /protein_id="HHQ1329467.1"
                     /translation="MQNTISVFSFKSQNVRTQILGAEPWFCLGDVAEILQIQNARQLP
                     LKDQGIQKSSVATKKGNQELLFINEPNLYRVIFRSRKAEAVKFQDWIFEEVIPQIRKT
                     GGYQITPKTTADDRTGLRRAVAALVGRKRIGYSSAYSMIHQRFNVEAVEGIPADKLPE
                     AVAYVHALTLHTGLAGEVPDREPLPAPQPALPISGNALADIAAMVYYGTRMIELGKDV
                     SAPLKQLGCKQAVTMWTVWHETRSILKRSVAALEVLRGYADKDASGRIAACLEGIYGK
                     AAAR"
     gene            complement(2059..2235)
                     /locus_tag="ACSIGJ_000511"
     CDS             complement(2059..2235)
                     /locus_tag="ACSIGJ_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329468.1"
                     /translation="MNPTKQSKKSYESKRVLKHVSFNTEKEANLLEFSNNLDFSKWVK
                     EKLKHELELEKLKK"
     gene            complement(2563..3054)
                     /gene="dtd"
                     /locus_tag="ACSIGJ_000512"
     CDS             complement(2563..3054)
                     /gene="dtd"
                     /locus_tag="ACSIGJ_000512"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HHQ1329469.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQRLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(3128..3886)
                     /locus_tag="ACSIGJ_000513"
     CDS             complement(3128..3886)
                     /locus_tag="ACSIGJ_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HHQ1329470.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQRPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAGMNRGDVTRNPVKLGERYYLFKLGAVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDALLEENGVKP"
     gene            complement(3956..4822)
                     /locus_tag="ACSIGJ_000514"
     CDS             complement(3956..4822)
                     /locus_tag="ACSIGJ_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HHQ1329471.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKDALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAVYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKRTGAPDGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(4871..5149)
                     /locus_tag="ACSIGJ_000515"
     CDS             complement(4871..5149)
                     /locus_tag="ACSIGJ_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HHQ1329472.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGLSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(5149..5439)
                     /locus_tag="ACSIGJ_000516"
     CDS             complement(5149..5439)
                     /locus_tag="ACSIGJ_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HHQ1329473.1"
                     /translation="MEYFMLLATDGEDVHEARMAARPEHFKRLETLKSEGRLLTAGPN
                     LLPDNPERVSGSLIVAQFESLDAAQAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(5442..5972)
                     /locus_tag="ACSIGJ_000517"
     CDS             complement(5442..5972)
                     /locus_tag="ACSIGJ_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HHQ1329474.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAAAAVALVAGVVQAAFLYWK
                     HKRLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPTVLFWCGALFLLGSHLAGKNGLK
                     ASIGREIQLPDAVWGKLTYMWVGFLIFMGIANWFVFTRFEAQWVNYKMFGSTALMLFF
                     FIIQGIYLSTYLKKED"
     gene            6280..8596
                     /gene="tspA"
                     /locus_tag="ACSIGJ_000518"
                     /pseudo
     CDS             6280..8596
                     /gene="tspA"
                     /locus_tag="ACSIGJ_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224879.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark-like protein
                     TspA"
     gene            complement(8769..9185)
                     /gene="gloA"
                     /locus_tag="ACSIGJ_000519"
     CDS             complement(8769..9185)
                     /gene="gloA"
                     /locus_tag="ACSIGJ_000519"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HHQ1329475.1"
                     /translation="MRLLHTMLRVGNLEKSLDSYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGDAYGHIAVEVDDAYEACERVKRQGGNVVREAGL
                     MKHGTTVIAFVEDPDGCKIEFVQKKSGDDSVAYANT"
     gene            complement(9210..10379)
                     /gene="lapB"
                     /locus_tag="ACSIGJ_000520"
     CDS             complement(9210..10379)
                     /gene="lapB"
                     /locus_tag="ACSIGJ_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HHQ1329476.1"
                     /translation="MDNELWIILLPIILLPVFFTMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGEMAREARQHLLNIYQQDRD
                     WEKAVETAQLLSHDEQTYQFEIAQFYCELAQAALFKSNFDAARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKGEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(10390..10704)
                     /locus_tag="ACSIGJ_000521"
     CDS             complement(10390..10704)
                     /locus_tag="ACSIGJ_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HHQ1329477.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINMDAVTFSYLPGQSVNLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENSRLRAEVKKSARLSGQKLTAPPIQNAAESAK
                     QP"
     gene            11014..12012
                     /gene="ilvE"
                     /locus_tag="ACSIGJ_000522"
     CDS             11014..12012
                     /gene="ilvE"
                     /locus_tag="ACSIGJ_000522"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HHQ1329478.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLTLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWLLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKNQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(12257..13042)
                     /gene="fabI"
                     /locus_tag="ACSIGJ_000523"
     CDS             complement(12257..13042)
                     /gene="fabI"
                     /locus_tag="ACSIGJ_000523"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HHQ1329479.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(13197..14018)
                     /gene="dapD"
                     /locus_tag="ACSIGJ_000524"
     CDS             complement(13197..14018)
                     /gene="dapD"
                     /locus_tag="ACSIGJ_000524"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HHQ1329480.1"
                     /translation="MSLQNIIETAFENRADITPTTVAPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNAVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGAIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSLPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(14228..15871)
                     /gene="pgi"
                     /locus_tag="ACSIGJ_000525"
     CDS             complement(14228..15871)
                     /gene="pgi"
                     /locus_tag="ACSIGJ_000525"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HHQ1329481.1"
                     /translation="MDAFTRAWYALERHYQDTCHILLRDRFAAEPDRFERMHERLDGM
                     LFDYSKNRFGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYADGRDVLPEIRRELNRALKFAHSLDDGLYQGITGKRIADFVHIGIGGSDLGPAM
                     CVQALEPFRRQISVHFVSNADPACLDEVLCRLNPETTMFCVASKSFKTPETLLNAEAV
                     KAWYRGAGFSESETARHFCAVSADTEAAQSFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAACKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGAEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPCNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            16381..17613
                     /gene="pilG"
                     /locus_tag="ACSIGJ_000526"
     CDS             16381..17613
                     /gene="pilG"
                     /locus_tag="ACSIGJ_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HHQ1329482.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGQVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPPLTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLFAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            17687..18547
                     /locus_tag="ACSIGJ_000527"
     CDS             17687..18547
                     /locus_tag="ACSIGJ_000527"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HHQ1329483.1"
                     /translation="MSDLSVLSPFAVPLAAVLGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEHLNLPLTDDESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYSSLWLLCAVYKLLTGKTGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGRHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVR"
     gene            18544..19176
                     /gene="coaE"
                     /locus_tag="ACSIGJ_000528"
     CDS             18544..19176
                     /gene="coaE"
                     /locus_tag="ACSIGJ_000528"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HHQ1329484.1"
                     /translation="MTAWVGLTGGIGSGKSAAAQYFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEIFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAVYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVADIISH
                     QASESERLLLADDVLLNDGSLKSLREKTMLLHAFYSGIFASKPTQGKHNG"
     gene            19169..19378
                     /locus_tag="ACSIGJ_000529"
     CDS             19169..19378
                     /locus_tag="ACSIGJ_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HHQ1329485.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDGAYR"
     gene            complement(19464..21140)
                     /gene="pilB"
                     /locus_tag="ACSIGJ_000530"
     CDS             complement(19464..21140)
                     /gene="pilB"
                     /locus_tag="ACSIGJ_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HHQ1329486.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIAVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVGILDVAYKEGMVDLRRAGILKIMQGITSLEEVTANTND"
     gene            complement(21386..21457)
                     /locus_tag="ACSIGJ_000531"
     tRNA            complement(21386..21457)
                     /locus_tag="ACSIGJ_000531"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21422..21424),aa:Arg,seq:cct)
     gene            complement(21478..21924)
                     /locus_tag="ACSIGJ_000532"
     CDS             complement(21478..21924)
                     /locus_tag="ACSIGJ_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HHQ1329487.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITVSATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGCG
                     VPLMGRQPVLVTGLLIGTVIGVAFMGGIPVGPLIAADILSFVAGKV"
     gene            complement(21928..22902)
                     /locus_tag="ACSIGJ_000533"
     CDS             complement(21928..22902)
                     /locus_tag="ACSIGJ_000533"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HHQ1329488.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIRVIQYKTAKLFEAAAQVGAILGKASPGHEQALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRGYFEKIRDYV
                     VRSDALAYSIGEARKAVDCAVAALDALSDSEVKDAMIQLAKESLVRVS"
     gene            23126..23434
                     /gene="rplU"
                     /locus_tag="ACSIGJ_000534"
     CDS             23126..23434
                     /gene="rplU"
                     /locus_tag="ACSIGJ_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HHQ1329489.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAQLDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            23459..23731
                     /gene="rpmA"
                     /locus_tag="ACSIGJ_000535"
     CDS             23459..23731
                     /gene="rpmA"
                     /locus_tag="ACSIGJ_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HHQ1329490.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKIDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            23966..25150
                     /gene="ubiM"
                     /locus_tag="ACSIGJ_000536"
     CDS             23966..25150
                     /gene="ubiM"
                     /locus_tag="ACSIGJ_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HHQ1329491.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEIVSQLDNVSILTDTAVKEVKTSDNEAQVFLENGDILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEKRLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(25339..25494)
                     /gene="rpmG"
                     /locus_tag="ACSIGJ_000537"
     CDS             complement(25339..25494)
                     /gene="rpmG"
                     /locus_tag="ACSIGJ_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HHQ1329492.1"
                     /translation="MRDKIKLESGAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(25526..25759)
                     /gene="rpmB"
                     /locus_tag="ACSIGJ_000538"
     CDS             complement(25526..25759)
                     /gene="rpmB"
                     /locus_tag="ACSIGJ_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HHQ1329493.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(26009..27532)
                     /gene="farB"
                     /locus_tag="ACSIGJ_000539"
     CDS             complement(26009..27532)
                     /gene="farB"
                     /locus_tag="ACSIGJ_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HHQ1329494.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFSVANAVSVPLTGFLAKRIGEVKLFTAAAAGFVIASWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRMLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKHRETATVRTPTDYVGLTLMMVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSAWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     QGISDGQTLGIINNTITQQGFIIGSNEIFLAGSILFIVLIPIVWLAKPPFHSGGGGH"
     gene            complement(27556..28710)
                     /gene="farA"
                     /locus_tag="ACSIGJ_000540"
     CDS             complement(27556..28710)
                     /gene="farA"
                     /locus_tag="ACSIGJ_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HHQ1329495.1"
                     /translation="METHTDETKLQNTQVKRKRRLTALTLLFALSAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RTAVSQAQAAVKAALAEESSARAALGGDVSLREQPEVQTAIGRLKDAWLNLRRTQVRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     regulatory      28951..28995
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28999..29472
                     /gene="queF"
                     /locus_tag="ACSIGJ_000541"
     CDS             28999..29472
                     /gene="queF"
                     /locus_tag="ACSIGJ_000541"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HHQ1329496.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIVIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAVHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            29547..30233
                     /locus_tag="ACSIGJ_000542"
     CDS             29547..30233
                     /locus_tag="ACSIGJ_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HHQ1329497.1"
                     /translation="MYALTAAQQQKALFRLVLFHILIIAASNYLVQFPFRIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     QFNTFVGRIALASFAAYALGQILDIFVFDKLRRLKAWWIAPAASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCTLFFLPAYGVILNLLTKKLTALQTKQAQ
                     DRPVPSLQNP"
     gene            complement(30583..31875)
                     /locus_tag="ACSIGJ_000543"
     CDS             complement(30583..31875)
                     /locus_tag="ACSIGJ_000543"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HHQ1329498.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTEGTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGSAREVQFFTDEDGERNLVALEKKGGIWRRSASDADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLLYDSLYFHGQQ
                     VAAGDILAAEVVKGDTTHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHS
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            31952..32920
                     /locus_tag="ACSIGJ_000544"
     CDS             31952..32920
                     /locus_tag="ACSIGJ_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329499.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSPHRQSCTASA
                     FYARYLWCGRLAQRPAQSLNMPSETVALATPVWQKMGLHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLNAHPVNHNRKKRGLPELNGLWLWDSLHGSAQGGTLFADTVWSRFH
                     PNRRALPDSFRAYAETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRTGKIKRLDIATDGQHGGTLTFKPTDRRKFWRCTKTFDGIW"
     gene            complement(33121..34584)
                     /locus_tag="ACSIGJ_000545"
     CDS             complement(33121..34584)
                     /locus_tag="ACSIGJ_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HHQ1329500.1"
                     /translation="MFYFYFCGKTFMPARNRWMLLPLLASAAYAEETPREPDLRSRPE
                     FRLHEAEVKPIDREKVPGQVREKGKVLQVDGETLLKNPELLSRAMYSAVVSNNIAGIR
                     VILPIYLQQARQDKMLALYAQGILAQAEGRVKEAVSHYRELIAAQPDAPAVRMRLAAA
                     LFEDRQNEAAADQFDRLKTEDLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNIN
                     QAPKQQQYGNWTFPKQVDGTAVNYRFGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKFN
                     DMTAGVSGGIGFADRRKDVGLAVFHERRTYGNDAYSYANGARLYFNRWQTPRWQTLSS
                     AEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWTGGLDFYRERNPADRGDNFNRYG
                     LRFAWGQEWGGSGLSSLFRLGVAKRHYEKPGFFSSFKGERRRDKESDTSLSLWHRALH
                     FKGITPRLTLSHRETWSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(34832..35220)
                     /locus_tag="ACSIGJ_000546"
                     /pseudo
     CDS             complement(34832..35220)
                     /locus_tag="ACSIGJ_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(35273..35524)
                     /gene="dptG"
                     /locus_tag="ACSIGJ_000547"
     CDS             complement(35273..35524)
                     /gene="dptG"
                     /locus_tag="ACSIGJ_000547"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptG"
                     /protein_id="HHQ1329501.1"
                     /translation="MYDEINITDNYRTDLKQAVKKQLQDLYKTRPEAWEENKKRVREE
                     IIKQFGISEDVFNKLQQKMPQKINSLIEEQVLTPYILRP"
     gene            35686..36174
                     /locus_tag="ACSIGJ_000548"
     CDS             35686..36174
                     /locus_tag="ACSIGJ_000548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329502.1"
                     /translation="MLHGPRQTWEFLSSAASKINLFLISASLTGSYASEPQAYGVQNS
                     DKTDNVNRLTRPLAFSVMRNFPLSSVKVIMIIYYITLHRNEKSGKTKNPSAVIPAKAG
                     IQNFNASRIYRGWPGFKGLGFRLRGNDGLEITRNEKKNRNRTSRITACAGMTAQKLRN
                     EK"
     gene            complement(36280..36525)
                     /locus_tag="ACSIGJ_000549"
     CDS             complement(36280..36525)
                     /locus_tag="ACSIGJ_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329503.1"
                     /translation="MLKSIELNSHIRNRLAEYLKGRGMDFQTAMQEEEGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            36637..37065
                     /locus_tag="ACSIGJ_000550"
     CDS             36637..37065
                     /locus_tag="ACSIGJ_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HHQ1329504.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGRWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            37077..37553
                     /locus_tag="ACSIGJ_000551"
     CDS             37077..37553
                     /locus_tag="ACSIGJ_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HHQ1329505.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GKQAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKRRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(37583..38071)
                     /locus_tag="ACSIGJ_000552"
     CDS             complement(37583..38071)
                     /locus_tag="ACSIGJ_000552"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HHQ1329506.1"
                     /translation="MLKITIIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETVASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAERTERRVSSKGVAYTFVHYL
                     KG"
     gene            complement(38139..39194)
                     /gene="aroG"
                     /locus_tag="ACSIGJ_000553"
     CDS             complement(38139..39194)
                     /gene="aroG"
                     /locus_tag="ACSIGJ_000553"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HHQ1329507.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDRRLLVIIGPCSIHDPKAALEYAERLLKLRKRYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAVEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIADQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANTKRMARAG"
     gene            complement(39356..39583)
                     /locus_tag="ACSIGJ_000554"
     CDS             complement(39356..39583)
                     /locus_tag="ACSIGJ_000554"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329508.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCPVPD
                     CRGNGKTDVPCGFARMNGGYCFYQSVSFSLK"
     gene            39811..40107
                     /locus_tag="ACSIGJ_000555"
     CDS             39811..40107
                     /locus_tag="ACSIGJ_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329509.1"
                     /translation="MRFKAEYARSKNDADDKRGNAHIANGAIGKMAAVYAGYTYTQPG
                     GESTRFRGGLERVQVKRYLGEKCCGYACPKSERFTLQADGEIRQNLGEGWFLKL"
     gene            40104..40343
                     /locus_tag="ACSIGJ_000556"
     CDS             40104..40343
                     /locus_tag="ACSIGJ_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329510.1"
                     /translation="MSGVAVYLRNKNKIAIASQDANLNSKGRFVSSGLNVGKQLTGSL
                     GVEFDPYYRHRAIRKSAEFVPNTTKTKTDSEKFNE"
BASE COUNT         9737 a        11427 c        10025 g         9435 t
ORIGIN      
        1 tatgggcagg cttcgcactc tgaaaggttc aggcggcatt tttattggaa ggcttttttt
       61 tgcaaccgcc ttacacaaag cggttttttg tgtaagaact gctataatag ccgcctgtca
      121 tcgtcaggag cggctaatgc ctttaaaatt ccaaccaagg gaacgttcag ttatcatgtg
      181 cgactttcgc ggttatgaag aaccggaaat ggtcaagaaa cgccctgtcg tcgtcatagc
      241 gcgaaacagg cacaacggca aaccggtaac ggtcgtaccc ttaagcagca cagagcctgt
      301 ccctttggcg gactgccacc acaaaatgag tgaaaacccc ttaccggaca agccgcacat
      361 ccaatgtcgg gcaaaatgcg atatgacggc aacagtcgga ttggcacgat tagaccgata
      421 caaacccaaa gggcgcgacc gttgcatccc aataatcagt gaagaggatt ttcaggcggt
      481 taaaacagcc gttgccaagg cattcaaact gtactagaat aaaaccgttc ccttaaaggg
      541 gcttgcaaga ctgttccgaa atatgggcag ccgcgcacgg gcgacaggcg atgacaagcc
      601 gtccgtgcgt gtgatggggc gcggaatgcg ccccttgtcg tatctgcaaa cgcctacaaa
      661 tccccaatca tcccttcaat aaaaatgccg tccgaacctt caggcggcat tttccgttta
      721 ccttgccgca gccttgccgt aaatgccttc aagacatgcg gctatgcggc cggaggcgtc
      781 tttgtccgca tatccccgca acacttcgag ggctgcgacg gatcttttca ggattgaacg
      841 ggtttcgtgc caaaccgtcc acatcgtaac cgcctgtttg cagccgagct gcttcagcgg
      901 cgcggaaacg tctttgccca attcgatcat ccgtgtgccg taataaacca tagcggcaat
      961 gtcggccaaa gcgttgccgc ttatgggcag ggcgggttgc ggcgcgggca acggttcgcg
     1021 gtcggggact tcgccggcca aacccgtgtg cagtgtcaat gcgtggacgt aggcgacggc
     1081 ttcgggcagt ttgtcggcgg ggatgccttc gacggcttcg acgttgaagc gttggtgtat
     1141 catactgtac gcggaggagt agcctatgcg tttgcgcccg acaagcgcgg caaccgcccg
     1201 gcgcaatccg gtacggtcgt cggcggtggt tttgggagta atttggtagc cgcctgtttt
     1261 gcggatttga gggataacct cttcaaatat ccagtcttgg aatttgacgg cttcggcttt
     1321 gcgtgaacgg aagatgactc ggtagagatt cggctcgttg atgaaaagta attcttggtt
     1381 gccttttttt gttgcaacgc tggatttttg aatgccctgg tcttttaatg gcaactgacg
     1441 ggcattttga atttgaagaa tttccgcaac atcgccaagg caaaaccaag gttcagcccc
     1501 caagatttga gtacggacat tttgagattt gaaagaaaat acagaaatgg tgttttgcat
     1561 tttaagctcc acgaaagttt gaaagcctct cttcgaccaa gaagaggtag gcggacgttt
     1621 caggttggtc gactgctcgt ggaaacagca catccggaga tgtcccgaaa gtccgcccgt
     1681 tggctttttc agcaagggtg tatgtttaac atacaccctt cagggaactc ttatcaaaac
     1741 aaaagccgca agcgcggcaa taaccacgaa agaaacaggc gaccaaacct gtgaaccgaa
     1801 ctttactatt attccgctat tgcgtcaata ttttgggggg aagcacggac ggagctttgt
     1861 tgaaagttta aaacttggac agtattcatg gtagaatgac ctttctattt catggtagat
     1921 agacgcggaa agagagttgc cgctctgctt tccacctgcc caaatcaagt tacagcttgg
     1981 tttgggcttc ttttttatct gcaatcagca agtgttgaaa ttataatttg tatctacgat
     2041 tcatgcaagc catttctatt attttttcag tttttctaat tcaagttcat gtttcagctt
     2101 ttcttttacc catttggaga agtctaaatt attggaaaat tccaataaat tagcttcttt
     2161 ttcagtgtta aatgaaacgt gctttaaaac gcgcttagat tcgtagcttt ttttggattg
     2221 tttggttggg ttcattcttc ccccttgttg aaaataatcg tagatacatt ttactcgatt
     2281 taaaatcata gggcaacaaa aaaaccgcca agtttgcggc ttggcggcgg tcgggctatt
     2341 ttaagaactc ttttcggatt ttttctttaa cccattggga aaaactaatt ttatttgcac
     2401 cttatttgca ccttttttat aaatataatt caaatatttg atttatatat taatttaatt
     2461 aaatattaca tgaccctgca taggggcgac ggaaaggcgg cgcgggggaa gggtgtgtgt
     2521 attgtcgttc attgtcgata tttcatcatt gcggactcaa tttcaatccg gaacaacctt
     2581 catttttggg gaaatccgcg tcatgaaaga gtccagcagt atggttaccg gcccgtcgtt
     2641 gcagagcgac acctgcatat gcgtgcggaa acgccctgtt tcgacatgaa tcccgtgtcc
     2701 gcgtaacagc tccgccgttc gcaggtaaag ccgttgcgcc tgttctgccg gcgcggcttg
     2761 ggaaaacgaa ggccgccgcc cgcttgccgc gtcggcataa agcgtaaact gcgacaccag
     2821 cagcaccgcg ccgccgacat ctttcaaaga caggttcagc ttgcccgctt cgtcttcaaa
     2881 cacgcgcaaa tgtgcgattt tgtcggcgat atagcgtgca tctttttctg tgtcgctatg
     2941 cgttacaccg agtaacacga caaacccgcc gtcgattttg ccgcaggttt ccgtgccggc
     3001 ttcggacacg acatccacct ttgcacctac cgttttctgt atgaccgcac gcataagccg
     3061 tcctccttac ctgaacgtgc aatgccgtct gaaaaggctt cagacggcat cggtatcgga
     3121 aatgccatta cggtttgaca ccgttttctt ccaaaagggc atcgattttc aagcgggctt
     3181 tttcctgcct caaaccttgt tccaactggt ttctgaccaa ctcgaaaggc tgcgcgtcgg
     3241 ggtttttccc gaccgcgccg agtttgaaca ggtaatagcg ttcgcccaat ttgaccggat
     3301 tgcgggtaac gtcgccacgg ttcataccgg caaactgcga agccagcggc tcgggaagct
     3361 gctgcgccat aatgaaaccg tcgaacgcct gctcgtcgtt cggataacgc ttcatcagcc
     3421 cttcaaaaga cagccctttg agcaggagct gctgcgcctg acgcgcctcc tcttcggttg
     3481 cgaagctgac ctgctgcaat ttgatcatgc ggatttgccg ctcataaaac tgacgcagtg
     3541 cgctttcgga aaccgtttcc gaacgttcca gaaaacggac gtactcctcg gcataaaaag
     3601 acgcttcggc gattttaaag cggttttgga catccttatc cttatccaaa ccttccttca
     3661 atgccctgtt tttcaaaact tccaaagttt gcagccggcg gacggcatcg tttcggattg
     3721 cctgcccgtc cggtctttgg gactgctccg catgccggtc tgcctgctgc atgatctgcg
     3781 ccaccagcgt atccaccaaa gccgggtcga tttcgggtgc tttggctgcc gcaaaacctg
     3841 ccaacattgc agcaataact gcggcagcgg tctttttctg tttcatggta ttccctgtcc
     3901 tgcgcggcgg caaaacgcct gtatgaacgt cttgccgccg tatcccgttt tcagattatt
     3961 ttgcaggttt gatgtttgcc ttacccaaca gcgcaccgac ggcacggtca atccgttccg
     4021 cctgaaggtt gccggcaatc tgtcctttca tttcgtcaaa agaaggcact ttcacctcgc
     4081 ggctgtcgtt gacataataa acgccgtaga aatcgccgtt tttcagcggc gttgccgtaa
     4141 attcgccttt tttcaagtcc ttaattgcct gataaagcgg cggaacaccc tgttccaaat
     4201 ctttcagcgg cacatatccg tccggcgcgc cggtccgttt ggtgcggtcg ttgagcgagt
     4261 attgtttcaa aacggcatca aaaccttttt tcgccttcaa atcggcaacc gcttttttcg
     4321 cattttcttc cttgtcggtc aggatttcgc ccaactggac ttcctgcgtg cctttataaa
     4381 aaccgctgat attgtcgtaa acggctttta cttcctgctc ggaaaccggt tgggttttgg
     4441 cgatatgcag tgcgtatgcc tcgccgttca agccatattt taccgcctgc caaacggttt
     4501 tgaaggacgg tttcttgtcg tcgcccgact ttttcgcttc ggcacgcaat ttggcaagcg
     4561 catctttaaa ctctgccgac cggtcgagtt tcaggcgttt cacttcctgt gcgaccacgg
     4621 tgttgaccac ttcgttttcc agcagggatt ggcgcagttg cggcgtgtct tcggcacggc
     4681 tgttttccgc acggaatgcg gcaacctgcg catcgatgac ggaactgtcg attttctgac
     4741 cgttaacggt tgccagcgtt tgggcaaaca ggctgccgga acaggcaagc agcgcaacgg
     4801 aagtcaggat ttttgctttc ataatcttct ctctttaaat tttaaattga aatattcagg
     4861 cagacttcat tcagtccgcc gtatggaaat actcgtcagg ggtagccgcc cggatgccga
     4921 gcgcgtgaat catgccgcct gaaaataaat ctttgagcag cgatttgacc gttttctgac
     4981 ggttcagacg gcttaaacct tcaaaacggc cgctgacgac caaaacggca taatgtccgc
     5041 cgccggtatt gcccgcgtgt cctttgtgca gatggctctc atcgccgatt tccaacacca
     5101 gaggatcgag cgtctgcagg cgttcgcgga tcaaatcgac agccggcatt atttgaacac
     5161 cgctttaaac ggcttgatca gcacttcgct gtacacgccg gcatgaacat agggatcgtc
     5221 ttcagcccaa gcctgcgccg catccaaaga ctcgaactgt gccacaatca agctgcccga
     5281 aacacgttcg ggattgtccg gcagcaggtt tgggcctgcc gtcagcagac ggccttccga
     5341 tttcagcgtt tccagccgtt taaagtgttc gggacgtgcc gccatacgcg cttcatgcac
     5401 atcctccccg tctgttgcca gcaacataaa atattccaca gtcaatcctc ctttttcaga
     5461 taggtactca gataaatacc ctgaataata aagaaaaaaa gcatcagcgc agtcgaaccg
     5521 aacatcttat agttaaccca ttgcgcttca aacctagtaa acacaaacca gttggcaata
     5581 cccataaaaa tcagaaaacc gacccacata tatgtcaatt ttccccatac ggcatccgga
     5641 agctgaatct ccctgccgat actcgctttc aagccgtttt tacccgcaag gtggctgccc
     5701 agcaggaata acgccccgca ccagaacaat actgtcggct tccacataat gaagcggctg
     5761 tcgcccaaaa caatggttgc gccgccgaat acgacaatca gcaccagtcc gacccactgc
     5821 atcgtatcca gccttttatg cttccaatac aggaaagccg cctgaaccac gcctgcaacc
     5881 aaggcaaccg ccgcagcggc aatcatattt ttggtaacgg tataagtagc aaaaaacaag
     5941 atgacggaca aaaggtcgct gacaaatttc atatccaaat gagaaagcaa atcaaataaa
     6001 cgctaatcat acaggcagaa ccctatcttt gcacctgcaa aaccaacaaa ttttgttttg
     6061 attaatgcaa atatgcacag atgtttttga aaaaagatgg aaatatgtca taatttataa
     6121 aattggcttt aaaatacagc ttcggtcgga acctctgcct atccgcaccc tatcgtccgt
     6181 attgcctatg cctgatgacg aaatttacag actgtacgcg gtcaaaccgt attcagccgc
     6241 caacccacag gggatacatc ttgcaaaaac aacagacaaa tcaaactgat tgccgcctcc
     6301 atcgcagttg ccgcatcctt tcaggcacat gcgcgactgg gcggactgaa tatccggtcc
     6361 aaccttgacg aacccttttc cggcagcatt accgtaaccg gcgaagaagc caaagccctg
     6421 ctaggcggcg gcagcgttac cgtttccgaa aaaggcctga ccgccaaagt ccacaagttg
     6481 gacgataaag ccgtcattgc cgtttcttcc gcacaggcag tccgcgatcc cgtcttggtg
     6541 ttccgcatcg gtgcaggcgc gcaggtacgc gaatacaccg ccatcctcga tcctgtcggc
     6601 tgctcgccca aaaccaaatc tgcactttca gacggcaaga cacaccgcaa aaccgctccg
     6661 aaagcagagt cccaagaaaa tcaaaacgcc aaagccctcc gcaaaaccga taaaaaagac
     6721 agcgcaaact cagccgtcaa accggcgcat aacggcaaaa cccataccgt ccgcaaaggc
     6781 gaaacgctca aacagattgc cgccgccatc cgtccgaaac acctgacgct cgaacaggtt
     6841 gccgatgcgc tgctgaaggc aaacccaaat gtttccgcac acggcagact gcgtgcgggc
     6901 agcgtgcttc acattccgaa tctgaacagg atcaaagcgg cagcacccaa aaaaatcaaa
     6961 gcggaacaac ccaaaccgca aacggcgaaa cccaaagccg aaactgcatc catgccgtct
     7021 gaaccgtcca aacaggcaac ggtagagaaa ccgatagaga aacctgtcga aaaaccggtt
     7081 gaaaaacctg aagcaaaagt tgccgcgccc gaagcaaaag cggaaaaacc ggccgttcga
     7141 cccgaaccca aacccgcagt gtccgaaact ccggcttcgg cgacagaacg ccaacccgga
     7201 cctgtacccg ctgcaaatac tgccgcatcg gaaaccgctg ccgaatccgc ccccccaaga
     7261 agccgccgct tctgccatcg acacgccgac cgacgaaacc ggtaacaccg tttccgaacc
     7321 tgttgcggaa acagccagtg aagttgccgc cgaacccgtt gccgaacctg tcgaacaggt
     7381 ttctgccaaa gaagaaaccg aaagcggact gtttgacggt ctgttcggcg gttcgtacac
     7441 cttgctgctt gccggcggag gcacggcatt gatcgccctg ctgctgcttt tacgccttgc
     7501 ccaatccaaa cgcgcgcgcc gtaccgaaga atccgtccct gaagaagagc ctgaccttga
     7561 cgacgcggca gacgacggca taaaaatcac ctttgccgaa gtcgaaactc cggcaacgcc
     7621 cgaacccgct ccgaaaaacg atgtaaacga cacacttgcc ttaggtgggg aatctgaaga
     7681 agagttatcg gcaaaacaaa cgttcgatgt cgaaaccgat acgccttcca accgcatcga
     7741 cttggatttc gacagcctgg cagccgcgca aaacggcatt ttgtccggcg cacttacgca
     7801 ggatgaagaa acccaaaaac gcgcggatgc cgattggaac gccatcgaat ccacagacag
     7861 cgtgtacgag cctgagacct tcaacccgta caaccctgtc gaaatcgtca tcgacacgcc
     7921 cgaaccggaa tctgtcgccc aaactgccga aaacaaaccg gaaaccgtcg ataccgattt
     7981 ctacaacaac ctgttctcaa acaaccatat cggcacagaa gaaacagctt ccgcaaaacc
     8041 tgccgcaccc tccggactgg caggcttcct gaaggcttcc tcgcccgaaa ccatcttgga
     8101 aaaaacagtt gccgaagtcc aaacaccgga agagttgcac gatttcctga aagtgtacga
     8161 aaccggtgcc gtcgcggaaa ctgcgcctga aacgcccgat ttcaacgccg ccgcagacga
     8221 tttgtccgca ttgcttcaac ctgccgaagc accggccgtt gaggaaaatg cagcggaaat
     8281 cactttggaa acgcctgatt ccaacacctc tgaggcagac gctttgcccg acttcctgaa
     8341 agacggcgag gaggaaacgg tagattggag catctacctc tcggaagaaa atatcccaaa
     8401 taatgcagat accggtttcc cttcggaatc tgtaggttct gacgcgcctt ccgaagcgaa
     8461 atacgacctt gccgaaatgc atctcgaaat cggcgaccgc gatgccgctg ccgagacagt
     8521 gcagaaattg ctggaagaag cggaaggcga cgtactcaaa cgtgcccaag cattggcgca
     8581 ggaattgggt atttgattcc caactgccct ttcgcagatc aaggatgccg tttcagacgg
     8641 catctttttt tgccttatcg gtgtaacgga taaagtttga accggcacag gctcaaacag
     8701 caggccgacg gcaacaaaat gccgtccgaa acccctaaag gcttcagacg gcattggcgg
     8761 cggcggtatc aggtattcgc ataggcaacc gaatcgtcgc cgcttttctt ttgaacgaac
     8821 tcgattttgc atccgtcggg gtcttctacg aaggctatca cggttgtgcc gtgtttcatc
     8881 aggccggctt cgcggacgac gtttccgccc tgccgcttca cgcgttcgca ggcttcgtag
     8941 gcatcgtcca cttcaaccgc gatgtgtccg taggcgtcgc ccaagtcgta tcgttccgta
     9001 tcccagttgt gcgtcagttc caaaaccgtg ctgtcggttt cgtcgccgta accgacgaag
     9061 gcaagggtaa atctgccttc gggatagtct tttcggcgga gcagtttcat acccaaaacg
     9121 ttttggtagg aatcgaggga tttttcgaga ttgcccacgc ggagcatagt atggagtaag
     9181 cgcatttttc gtgttcctct cggtggtggt taaacttcga ttttattcgg cgtaaacgtc
     9241 tgccatttgt tgcaggcggg acagtgccag aaaaagactt gggatttgaa gtggcagttg
     9301 cggcaacggt acatcacgct gcgctggagc tgccgtccga taaccgaacg catcatgtcg
     9361 gcatcggctt tccaagccgg atccaaatcg ctgagtttca aaccgagcag gcggtacacg
     9421 ccgttaaggt cgggcttgcg gcggacaagc tcgacggcgg tttgcgcggc ttctttctcg
     9481 cccttaagca gcagggattt ctcgtacacg acattgatca ggtcaagttc gggaaacgtc
     9541 tgcatatatc ctgtcagacg gttcaagcct tcttcaggtt ttccctgcgc ggcataggct
     9601 tcgtaaagct tctcgccgac catgctcaag tatgcatggt tttgctgctc gatggcggca
     9661 taggcttcga cggcggcagg gaaattgcct tgtcggtgtt caatgtcgcc caaaatcatg
     9721 ttggcgcggg tgcatttttt gttggcttcg agtgccttgc cgacattgaa acgcgcggca
     9781 tcgaaattgg acttgaacag cgcggcttgg gcaagttcgc aataaaactg tgcaatctca
     9841 aactgatatg tctgttcgtc gtgactaaga agttgggcgg tttcaaccgc tttttcccaa
     9901 tccctgtcct gctggtagat attgagcagg tgctgtctgg cttcacgcgc catttcaccg
     9961 tcttgcagcc ccaaaaaaat ctgttcggca cgatcgacca aacccgcgct ttggtagttt
    10021 tgcgccaatt caaacaggac gcgcgcgcgc ttttcgccga ccgtatcggg agaatcgagc
    10081 attgtccggt gtatgttgat ggctttgtcg ttttcgccgc gctgacggta aagtttgccg
    10141 agggtaaggt tcaaatcata cgattgcggc cggccgtcga cgacttccgc caactccctt
    10201 gccgcgcgcc cgctgttgcg gtcgaccaaa gcgtccaggc ttttataaaa tcccgaaggg
    10261 atgctttttg cctgcttcaa tacggttttc atatccacgc gggcggcaaa ccagcccatc
    10321 gtgaagaaga cgggcaaaag gataatcggc agcaggataa tccacaattc gttgtccata
    10381 tcggctttct taaggctgtt tggcagattc ggcagcattt tgtatcggcg gtgcagtcaa
    10441 tttctgtccg ctcaagcgcg cacttttctt cacttccgca cgcaggcggc tgttttcgcc
    10501 gcgcaaggac agcagccgcc cgaacagggc aaacattccg aacacgatgc cgacgacaaa
    10561 cgcgccgaac aatacgacaa tcagcggcag attgacactc tgccccggaa gataggaaaa
    10621 ggtaacggca tccatattaa tgacggcaag cagcaggaag agcagcagga taatgatttt
    10681 gatgacggta tagataagtt tcatgtttgc tccgtaaata aaaacgccgc acggacagct
    10741 tcgcactctg aaccaagatt gcgttagttt aaacgatttg cacggtttcg gatagaatgc
    10801 ggcagcgtgt tcggacggca tacggagtat gcgattgccg acaattttac caaatacacc
    10861 gctcctttcc atttgaaaaa taacggattg gacaccgcat cgacagaaaa acccgccgcg
    10921 cacttgtcaa aaccctgttt gcaggcgtat ctttacaacc ttcaaattca aaccgttcat
    10981 tgaaacatat cagaataaga aaggctttac atcatgagca gacccgtacc cgccgtattc
    11041 ggcagcgttt ttcacagtca aatgcccgtc ctcgcctacc gcgaaggcaa atggcagccg
    11101 accgaatggc aatcttccca agaccttacc cttgcacccg gcgcgcatgc cctgcattac
    11161 ggcagcgaat gctttgaagg tttgaaggcc ttccgtcagg cagacggtaa aatcgtgctg
    11221 ttccgtccga ccgccaatat cgcgcgtatg cggcaaagtg cggacatttt gcacctgccg
    11281 cgccccgaaa cccaagctta tctcgatgct ttggtcgaac tggtcaaacg tgccgccgac
    11341 gaaattcccg atgcgcctgc cgcgctgtac ctgcgcccga ccttaatcgg taccgatccc
    11401 gttatcggca aggccggttc tccttccgaa accgccctgc tgtatatttt ggcttccccc
    11461 gtcggcgact atttcaaagt cggctcgccc gtgaaaatct tggtggaaac cgaacacatc
    11521 cgctgcgccc cgcatatggg ccgcgtcaaa tgcggcggca actacgcttc cgccatgcac
    11581 tggttgctga aggcgaaagc cgaatacggc gcaaatcaag tcctgttctg cccgaacggc
    11641 gacgttcagg aaaccggcgc atccaacttt atcctgatta acggcgatga aatcattacc
    11701 aaaccgttga cagacgaatt cctgcacggc gttacccgcg attccgtgct gactgtcgcc
    11761 aaagatttgg gctataccgt cagcgaacgt aatttcacgg ttgacgaact gaaagctgct
    11821 gtggaaaacg gtgcggaagc gattttgacc ggtacggccg ccgtcatctc gcccgttact
    11881 tccttcgtca tcggcggcaa agaaatcgaa gtgaaaaacc aagaacgcgg ctatgccatc
    11941 cgtaaggcga ttaccgacat ccagtacggt ttggcggaag acaaatacgg ctggctggtc
    12001 gaagtgtgct gatgctttaa ataaaaaaat gccgtctgaa acccgtttgg cgtttcggac
    12061 ggcattttcg cgttcggacc gtttccgccg cacctgcagc aagtcggtac aaaggcaatc
    12121 ggttaaaaca agcgtccgca tttcccatcc cgcctgccgc aagtcgggca tttccctgga
    12181 aatacgcttc aaacggcaaa ccgccgcccg aaaccgatac tcggcacgga cggcgcacgg
    12241 atttgaaaac ggcggattat ccctcggtgc tcaaggcatt gatgctgtaa ccgccgtcaa
    12301 cataagtgat ttcgccggta atgccggacg acaggtcgga cagcaggaag gcggcagtat
    12361 tcccgacttc ttcgatggta acgttgcggc gcagcgggtt gtgggcggcg acgtgtccca
    12421 agagtttgcc gaaatcggcg atgccggagg cggcgagcgt tttgatcggg ccggcggaaa
    12481 tgccgttgca gcggatgccc tctttaccca gacaggcggc ggtaaagcgg atgcctgcct
    12541 caaggctggc ttttgccata cccatcacgt tgtaattcgg aatcgcgcgt accgcgccca
    12601 agtagctaag ggcgacgatg gcggagtttc tgccgcgcat catcggacgg gcggcttttg
    12661 ccaatgcggg caggctgtat gcggaaattt cgtgtgcggt gttgaacgct tcgcggctga
    12721 tgctgtcgag gaagtcgccg ctcaaggctt ctttcggcgc gaagccgatg gaatgcacca
    12781 aaccgtccaa gccatcccaa tgtttgccta agtcggcgaa cacttggttg atttcgtcgt
    12841 cgctggcgac atcgcagcgg aatacgagtt cggaatctaa ttccgccgcc attttgcgga
    12901 cgcgctcttc cagtttgtcc acaacgtagg taaacgccag ttccgcgccc tgttcgcggc
    12961 aggctttggc gatgccgtaa gcgatggaac gctcggaaat catgccggta atcagaattt
    13021 ttttgccttg cagaaaaccc attttgtatc cttcaaatag tgttcggttc gttctaaacc
    13081 gttgattata gcaaattgtc cctgtttctg tgttttcacg ttgcagcgtg cagacggcaa
    13141 tgccgtccga agcggatttc agacggcatt ggacgtttca aatacggttt aaggcatcag
    13201 atgccgcgca agagttcgtt cacgctggtt ttggcgcggg tttgcgcgtc cacgcgtttg
    13261 acgatgacgg cacagtaaag gctgtggctg ccgtctttgg aaggcaggct gccggacacg
    13321 acgaccgaac ctgccggtac gcggccttgg tagatttcgc ctgtggtgcg gtcgaagatt
    13381 ttggtggatt gaccgatgaa cacgcccatc gaaatcacgc tgccttcttc gacaatcgcg
    13441 ccttcgacga tttcggaacg cgcgccgatg aagcagttgt cttcaatgat ggtgggtgcg
    13501 gcctgcaggg gctcgagtac gccgccgatg ccgacaccgc cgctcaagtg gacgtttttg
    13561 ccgatttgcg cgcaagagcc gacggtcgcc caagtatcga ccatcgcgcc ttcgtcgacg
    13621 tatgcgccga tgttgacgta agacggcatc agcacggcgt ttttggcaac aaagctgccg
    13681 cgtcgggcaa ccgcacccgg aactgcgcgg aagcctgcgt ttttgaactc gtcttcagac
    13741 cagtcggcaa atttggtcgg cactttgtcg aagtatttgt tcacgccgtc gttgaggact
    13801 tcgttgtctt ggatgcggaa ggacagcaac acggcttttt tcgcccattc gttgactttc
    13861 cactcgccca cgcccaagcg ttcggcgacg cgcagtttgc ctgaatcgag ttggcggatg
    13921 gtttccaaca cggcttcttt gacttcgggg gcaacggtgg tcggagtgat gtccgcgcgg
    13981 ttttcaaagg cggtttcgat aatgttttgc aaagacataa tgtttccttg gttcgagata
    14041 tttcgggacg cttgccggta ttgttcagac ggcatggcgg ttggaacaaa cgtgctattg
    14101 tgccatacaa acgcccgccc gtcagccgcg ccgcaggctt gccggatgcc ggcgtttcgg
    14161 aatcgggcgg gcggcaggca tatgtctttt tccgcgcccg aatcaataca gaaaggcggc
    14221 ggtactttta tgccgcgccg ccgcctttca gacggcattc gcggtaaaac gccatcagcc
    14281 cttcggtcga ggcatcgtgt acggacgtgc cgccttccag ttcgccgatg atggtttttg
    14341 ccaactgttt gccgtactca accccccact gatcgaaggg gttgacgttc catatcgcgc
    14401 cttggacgaa ggttttgtgt tcgtaagccg ccatcagcat acccagattg cagggcgtga
    14461 ggcggtcgag caggatgctg ttgctggggc ggttgccggg gaactctttg tgcggcgcga
    14521 ggcgttcgcg ttccgcttcg ggcaaatctg ccagttcggc gcgtgcttcg tctaaggttt
    14581 tacccttcat cagggcttcc gcttgggcaa aggcgttggc aacggtaaaa cggctgcgtc
    14641 cgtcctccgc gccctgcgcc gtcatcggga cgataaagtc gcagggaatc aggcgcgtgc
    14701 cttggtggag cagttggaaa taggcgtgct ggcagttgac cccttcaccg ccgaacacga
    14761 tgccgcccgt tttgcacgcg gcgggactgc cgtctgaagc gcggcttttg cccaaactct
    14821 ccatatcgag ctggttcagc cacgccggca gcaggcgcag gttgtggctg tacggaacgg
    14881 cggtctgccc gtccgcgtgc tggaaattgt tataccacac ggcaatcagt gccatcagaa
    14941 cgggaatgtt gcgacgcggg ggcgtgtgga aaaaatggct gtccatcgcg tgcgcccccg
    15001 ctagcagttc gcggaaacgc gccccgccaa ccgcaaccat cacgggcaaa ccgacgggcg
    15061 accagacgga atagcgtccg cccacccagt cgtacatcgc aaacacgcgt tccgccgcaa
    15121 tgccgaaact ttgcgccgct tccgtatcgg cagacaccgc gcaaaaatgg cgcgccgttt
    15181 cggattccga gaaccccgca ccgcgatacc acgccttgac cgcctctgca ttgagcaggg
    15241 tttccggtgt tttaaaggac ttgctggcaa cgcaaaacat tgttgtttcg gggttcagac
    15301 ggcataagac ctcatccagg caggcagggt cggcattaga aacaaaatgg acggaaatct
    15361 gccgtctgaa cggctcgagc gcctgcacgc acattgccgg cccgaggtcg gatccgccta
    15421 tgccgatgtg gacaaaatcc gcaatccgtt ttccggttat cccctgatat aaaccgtcgt
    15481 ccaaactgtg tgcaaacttc aacgcacgat ttaactcgcg gcggatttcg ggcaacacgt
    15541 ccctgccgtc cgcataaacg gcatccgcac cgtcgggcag gcgcaaagcc gtatgcagcg
    15601 cggcacgccc ctcgctgccg ttgactttcg cacccgtccg caaagcacgc attttccctt
    15661 ccaaatccgc cgtctccgca agacggcaga gcagttgcag cgtatcttcg ccaaaacggt
    15721 ttttgctgta atcgaacaac atcccgtcca aacgctcatg catacgctca aaacggtcgg
    15781 gttcggcagc aaagcggtcg cgcaaaagga tgtggcacgt atcctgataa tggcgttcga
    15841 gcgcatacca tgcacgggta aaggcatcca tctgttttcc ttgatttttc agaaccggat
    15901 taaaatatag cagaatgtag tctaacaaac ggcagacact ttagcgaatc tcaggaacac
    15961 cgcccgccgc gtccaataac accttatttc catatggcga aaatatagtg aattaacaaa
    16021 aatcaggaca aggcggcggg ccgcaggcag tacggatagt acggaaccgg ttcgcttggt
    16081 gcttcatcac cttagggaat cgtcctcttt gagccggggc gggccaacgc cgtaccggtt
    16141 tttgttaatt cactataccc tccggccggc atttgacccg caacaatatc ctgcaagatt
    16201 tattgtgtgc gcataaatgc cggacagccg cctaaattaa ggtataatcg catccgattc
    16261 tgtcccgttt gccgcccgca ggcgggatgt cggcggtttt catcttaccg ggaacacgcc
    16321 gatactgttt tttaacgatg tttcttacat tttattccaa ttactttacg gggctagaat
    16381 atggctaaaa atgggggatt ttctttgttc gcaaagaaag aaaaacgctt tatctttgaa
    16441 ggcaggcatt ctgcctccga caaactggtc aacggcgaag tatccgcgtt taccgaagaa
    16501 gaggcacgca aaaaactggc aaaacgcggc atccgcccgt tgcagattac ccgtgtgaaa
    16561 acaagctcca agcgcaaaat cacacaagaa gacattaccg ttttcacccg ccagctttcc
    16621 acgatgatta aagcgggcct gccgctgatg caggcatttg aaatcgtggc gcgcggacac
    16681 ggcaacccgt ctatgacgga aatgctgatg gaaatccgag gccaagtgga acagggcagc
    16741 tcgttgagcc gcgcattctc aaaccacccg aaatatttcg accgcttcta ctgcaacctg
    16801 gttgcggcgg gcgaaacggg cggtgtattg gaaagcctgc tggacaaatt ggcaatttac
    16861 aaggaaaaaa cccaggccat ccgcaaaaag gtaaaaaccg cgctaaccta tccggtatcc
    16921 gtcatcgccg tcgccatcgg tttggtattc gtgatgatga ttttcgtact gcccgccttt
    16981 aaagaagttt acgccaatat gggcgcggag cttcccccac tgacccaaac agtgatggat
    17041 atgtccgact ttttcgtctc atacggctgg atggtgctga tcgcactggg ctttgccata
    17101 tacggcttcc ttaaattgaa ggcgcgttcg attaaaatcc aacggcgtat ggatgccata
    17161 ctgctgcgta tgccgatttt cggagacatt gtccgcaaag ggacgattgc ccgctggggc
    17221 aggacgacgg caacgctgtt tgccgccggc gtgcctttgg tcgatgtatt ggactccact
    17281 gccggcgcgg cgggcaattt aatctatgaa gaagccaccc gggaaatccg tacgcgcgtc
    17341 atccaaggtc tgtctatgac ttcggggatg cgtgcgacgg aactgttccc caatatgatg
    17401 ttgcagatgt cctccatcgg cgaggaatcg ggttctttgg acgatatgct caacaaagcc
    17461 gccgaatttt acgaagacga ggtggacaat gcggtcggca ggctgtccgc tatgatggag
    17521 ccgattatta ttgtgatttt gggcttggtc atcggtacgc ttctggtcgc tatgtatctg
    17581 ccgctgttca acttgggcaa cgtggtcgcc tgatttgccg cacagatccg gcgcggattg
    17641 gttctgcgcc ggtttgtttt tgctttgaat atatcaagga caaaatatgt ctgatttgtc
    17701 tgtattgtcg ccgtttgccg tgcctttggc agcagttttg gggctgctgg tcggcagctt
    17761 cctgaatgtc gtcatttacc gcgtacccgt tatgatggaa cgcggctgga cggtatttgc
    17821 caaagaacat ttaaacctgc cgctgaccga cgatgaaagc cgtaccttca acctgatgaa
    17881 gccggattcc tgctgtccca aatgccgtgt gccgatacgc gcgtggcaga acatcccgat
    17941 tgtcagttac ctgctcctgc gcggcaaatg cgcttcctgc caaaccaaaa tcagcatacg
    18001 ttatccctta atcgagctgc tgaccggcgt attgttcggg ctggtcgcct ggcaatacgg
    18061 ctggtcttgg attacgctgg gcggtttgat actgaccgcg tttctgattt ccctgacctt
    18121 tatcgatgcg gacacccaat acctgcccga ctcgatgaca ttacccttga tctggctggg
    18181 gctgatattt aatttggacg gcggcttcgt gcctttgcag tctgccgttt taggtgcggt
    18241 tgccggctat agttcattat ggctcttatg tgcagtgtat aaactgctca caggaaaaac
    18301 cggtatgggc aacggagatt tcaaactgat tgccgcattg ggcgcgtggc tcggcatatc
    18361 cgcattgccc gtgctgattt ttgtttcctc tctgatcggt ttggtcgcgg caatcgttat
    18421 gcgcgtcgcc aaggggcggc attttgcctt cggccccgca ctgacagttt cgggctggat
    18481 aatttttacg gcaaacgatt ccgtatggcg ggcggtcaac tggtggctga cccatccggt
    18541 gagatgacag catgggtcgg actgaccggc ggaatcggca gcggcaaatc ggcagccgcg
    18601 caatattttg ccgatttggg cgtgccgcgc atcgatgcgg acgcggcggc gcactcgctg
    18661 acggcttcag acggcatcgc cctgccggaa atcaggcggc tgttcggcga caccgttttc
    18721 gacacacagg gtttgttgcg gcgcgacata ttgcgtaaag aaatctttgc ctccccatcg
    18781 cgcaaagcct tgctcgaatc cgtgatgttg ccgctgattt tctcagaaat caaaaaacag
    18841 caagaaacct ttaccgatgc ggtttacggc attgtcgaaa ttccgctgct gacggagaag
    18901 cgtcaattta tcagcctgat acggcgtgtc ctgaccataa gcgcaccttt ggaaaaacgt
    18961 atcggcaggg taatggcacg cagcgggctg acgcgcggcg aggtcgcgga catcatcagc
    19021 catcaggcat ccgaatccga acgcctgctg cttgcagacg atgtactgct caatgacggc
    19081 agcctcaaaa gcctacgtga gaaaacaatg ctcctgcacg cgttttattc agggattttc
    19141 gcctcaaaac caacacaagg aaaacacaat ggctgaatcg cggcaaacac gccttcaagt
    19201 caaatgtccg acctgtcaaa cggcagtagt atggaaaccg gaaaacgcat tccgcccgtt
    19261 ctgttcgcaa cgctgcaaac tgatcgactt gggcggatgg gcagacggga aatatacggt
    19321 ttccggccaa acggaaagtt tgccggaaat atccgaaccc gacggggcat accgctgacc
    19381 gccccgcctt cccggcaaac accctgaaag gtcaaatgcc gtctgaaaca aacacgcttc
    19441 agacggcatt ttcattctca aacctaatcg ttggtatttg ccgttacctc ttccaatgaa
    19501 gtaatgccct gcataatttt caaaataccg gcccggcgca aatccaccat accctcctta
    19561 taggcaacgt ccaaaatacc cacttccgta ccgttgttca taatcacacg ctgcatttct
    19621 tcgctgatgg gcataacctc atacacgccc gcacgcccct tataaccctg cccccggcaa
    19681 cggtcgcaac cgacggcgcg gtaaagtttc caatcttttg caagatcctc atcggtgaaa
    19741 ccgacttcct tcaaagcaga ggcagacggg cgttccactt cctgtttgca gctcgaacac
    19801 agcctgcgta aaagacgctg cgccataatc aggctgaccg aactggcaat attaaacggc
    19861 gcgacaccca tattcagcat acgcgacaac gtcgccggcg cattattcgt gtgcagtgtg
    19921 gaaaacacca tatgccctgt ttgtgccgcc ttaatcgcaa tatcggcagt ttccaaatca
    19981 cgaatctcac cgaccataat gatgtccggg tcctgacgca ggaaagactt caaagcagcg
    20041 gcaaaagtca gaccctgctt atcattgacg ttaacctgat tgatgcccgg caggttaatc
    20101 tcggcagggt cttccgccgt tgcaatattt accgactccg tattcaaaat attcaaacag
    20161 gtatagagcg acaccgtctt acccgaaccc gtcgggccgg ttaccagcac catcccgtaa
    20221 ggacggtgaa tcgcttccaa caacaatttt ttctggaacg gctcaaaacc gagctggtcg
    20281 atgttcaaag acgcggcatc ggaattcaaa atccgcatca cgaccttttc gccaaacagc
    20341 gtcggcaatg tgctgacacg gaaatcgaca ggcttgccgc ccttttgaaa ggtcagctgc
    20401 atcctaccgt cctgcggtat ccgtttttcg gaaatgtcca aacgcgacat taccttaatc
    20461 cgggaagcaa gctgccccct taccgcaatg ggcggctgaa ccacctcgcg gagctgcccg
    20521 tccacacgga aacggatacg cgcattgtgt tcgtaaaact cgaaatggat gtcggatgcc
    20581 ccgctacgca aggcatccga caaagtttta tggataaacc tcggaacagg gccgtcttct
    20641 gcctcctcgt tgtcgatata cagggtgtgg ctttcctctt cctcttgccc ctccccaagc
    20701 tcctgaagca gcgatgtcga acgcgaaccc acccaatcga gcaaacccgc caactggtca
    20761 tcctcgacaa tgaccaactc aaccgcaatc cctgcggcag aaacggtttt ctgaatttgc
    20821 ggcatctggg tcggatcgga aaccgcaaaa aatactttgt cgccccgacg gaaaaccggc
    20881 acacagtgga actccaccat ctgctcctcc gtcaacaccc ccatcagcac cctgtggcgc
    20941 ggataatgac gcaaatcaag aatcgaataa ctgaacaccc tcgcaatcaa tgccgcaagc
    21001 gacttgggcg aaatgacacc gtctgaaaac agcatcggca acacttcctt acccgcctgc
    21061 gactcattgt agtaatgctc ggcctgctca acagtaacca cctggttttg aaccagaatc
    21121 ctcagcaaac ctacgctcat acgaccttat ccccaaattt attcattgtt atacctgtac
    21181 agattttatc ataaaacaac agatttccaa gcctgaacat cttttacgaa gcctgaaaat
    21241 catttcacga ttctgccgtc ctaaaggtcg gtttttcaag caggaagaaa aagttttcag
    21301 atggcaaaaa agccctccag cactgaaagg ccttatatcg gaaacttccc gcaacacggg
    21361 aaacagacaa atgcaaaccg tcaaacctcg ccaacaggaa tcgaacctgt attttacgct
    21421 taggaggcat acgttctatc cgttgaacta tggcgagccg aaatgaaaag ggagatttta
    21481 aacctttccg gcaacaaaag acaagatgtc ggccgcaatc agcggcccga cagggatacc
    21541 gcccataaat gccaccccga tgaccgtacc gatcagcaag cccgttacca ataccggctg
    21601 ccgtcccatc aaaggcacgc cgcatcccgc cagccaagcc acgaaaatac cgataaaaac
    21661 ggcggatatc attttaaaat tcaaaaattc ggcaacagga ggaacctgcg cctttcctga
    21721 aaccaacgga ctcaaaaccc ctatggtcaa aagaatgata ccgaggttca acccgtgctt
    21781 ctcgaccaac gggacaaact gtaccaatgc cgtctgctgc atcagcagca atatggttgc
    21841 cgagacggta atcgaattgt tgttgctgac cacccccaac agaatcagcg taaccagaaa
    21901 caggggaaca aaactgaaat tcatcggtta cgacaccctg accaaagatt ccttcgccag
    21961 ctgaatcata gcatccttca cttcgctgtc ggacagggca tccaacgcgg caacggcaca
    22021 atcgactgct ttgcgcgcct cgcctatcga atatgccaaa gcatccgaac ggacgacata
    22081 atcgcggatt ttttcaaaat agccgcgatc tgcattttcc aaagcagtac gcacatcgtt
    22141 cgcaacctgt tcggaaccct gacgcatcag ataaatcaaa ggcagggtcg gttttccttc
    22201 cgccaaatcg tcgccgacgt ttttacctgt ttcttcggtt tcccccgaat aatccagcac
    22261 atcgtcaata atctggaatg ccgtaccgac gtacataccg tagtctttca aggcctgttc
    22321 gtgcccgggg gaagctttgc ccaaaattgc gccgacttga gcggcagctt caaacaattt
    22381 tgccgtttta tattggatga ctcggatata ttgttcttcg gtaatgtccg tattgccgat
    22441 gttcatcagc tgcatgacct ctccctcggc aatgatgttg gtcgcatccg ccatcacttc
    22501 caaaacgcgc atactgcccg aggcaaccat cagttgaaat gcacgggtat ataaaaagtc
    22561 gccaaccaac actgccgccg cattgccgaa cagattgttt gccgttgccc gcccacggcg
    22621 caaatcgctt tcatcgacga catcgtcgtg caggagtgtg gaagtgtgga taaactcgac
    22681 catcgctgcc agcgaataca gtttctcgtc atcataaccg accgccttac ccgccaaaat
    22741 cgtcataatc ggacgcaggc gtttgccgcc cgcgctgatg atatatgtac cgatttgcga
    22801 aatcagtgcg acatcggatt gcaccgcacg gttgatgact tcattgactt tggcaaggtc
    22861 ttcaggcaga tgtcgctgga aatagggcag attctcgagc atggcagact ctcaggttta
    22921 cgtttccgtc aaacggcaga taccgcttga aagctgattc aaatagcggg caattatatc
    22981 agcaaagcca tcagggtaca tcttttcaga cggcatatga ttgcccgaac cgcgcaaact
    23041 ttgacaaaac aggcaaataa caatacaatg cccaatttcg cacaatggtg tgcggatatt
    23101 aaccataacc cttatggagt tgagtatgta cgcggtcgta aaaaccggcg gcaaacagta
    23161 taaagtttcc gtcggcgaaa aattgaaagt agaacagata ccagcccaac tcgacagcca
    23221 aatcgaactg accgaagttt tgatgattgc tgacggcgaa tctgtaaaag ttggcgcacc
    23281 ctttatcgaa ggtgcaaaag taacggctaa agtagtggca cacggtcgtg gcgaaaaagt
    23341 ccgcatcttc aaaatgcgcc gccgcaaaca ctaccaaaaa cgccaaggcc accgccaaaa
    23401 tttcacccaa atcgaaatcg tggcaatcgc ctaaccttaa aacttaggag cattacaaat
    23461 ggcaagtaaa aaagcaggcg gcagcacccg caacggtcgc gattcagaag ccaaacgctt
    23521 gggcgttaaa gcctacggca acgagctgat tcccgcaggt tccatcatcg tacgccaacg
    23581 cggtaccaaa ttccacgcag gcgacaacgt aggtatgggc aaagaccaca ctttgttcgc
    23641 caaaattgac ggttatgtcg aattcaaaac caaaggcgcg ctgaaccgta aaactgtcag
    23701 catccgtcct tacaccggtt ctgaagaata attcaaccga tacctgaagc cgcatctttt
    23761 cacgatgcgg ctttttacat accccatacc cgatttgata cacattaagc tgaaagtaaa
    23821 aatccgcata caccctcccc tgcatatttc ttcaacaacg ggttttgata taatcgccta
    23881 tctgttacag atagttcaaa cggcattcaa acccttacaa atgccgtctg aaatcatcgg
    23941 catccgccta tgcaaaggat attttatgag tttacacagc gacatcctcg tcgtcggcgc
    24001 aggcccggcg ggtttaagtt ttgcagccga acttgccggc agcggtttga aagttactct
    24061 gatcgaacgc agcccactga ctgttctgca aaatcctccc tatgacggac gcgaaatcgc
    24121 cctgacccat ttttcccgcg aaatcatgca gcgtttgggt atgtgggaca aaattcccga
    24181 aaacgaaatc taccccttgc gcgatgccaa agtgctgaac ggacgttccg actaccagct
    24241 ccactttccc caaccgactg aagcgcgcgg cgagcctgcc gactgtttgg gctatctgat
    24301 ttccaaccac aatatccgcc gcgccgccta tgaaatcgta tcacaactcg acaatgtcag
    24361 cattctgacc gatacggccg ttaaagaagt caaaacatct gataatgaag cgcaagtctt
    24421 tttggaaaat ggagacattc taaccgcgcg tctccttttg gcagcagaca gccgtttctc
    24481 acaaacccgc cgacaactcg gtatttcttc agatatgcac gactacagcc gaaccatgtt
    24541 cgtctgccgc atgaaacata ccctttccaa ccaacatacc gcttacgaat gcttccatta
    24601 cggacgcacc atcgcactgc tgccactgga aaagcgcctg accaataccg tcattacagt
    24661 cgataccgat aaaatcaaca gcgttcaaaa cctttcgccc gaagaattgg cggcaagcgt
    24721 aaaagaacag ctcaagggca ggctcggcga catggaactg gtcagcagca tccaccatta
    24781 cccccttgtc ggcatgattg ccaaacgttt ctacggcaaa cgcagcgcgc tgatcggcga
    24841 tgcggctgtc ggtatgcacc ccgtaacggc acacggcttc aacctcgggc tttccagcgc
    24901 ggatattctg gcaaaactga tactcgaagc cgaacagcgc ggtcaagaca tcggcgcggc
    24961 ttccttgttg gaaaaataca gcagcaaaca tatgctgcac gcccatccgc tttatcacgg
    25021 caccaatatg atgctgaaac tgtttaccaa cgaaaccgcg ccggcaaaac tgttgcgcgg
    25081 tttggtatta cgagccggca acaacttccc gccgctgaaa aagctgatta ccaaacaact
    25141 gaccggttga aaactgccga aatgccgtct gaaacgtttc agacggcatt tttgtattat
    25201 gaaccgtttt gcggtttcca tatccttggc agacggtttg cagcagattc gcatacggca
    25261 gatgtttcaa gcagaaagga aacacaataa aaataaaaaa gcctccgaac agtcggaggc
    25321 tttgctttca aactgaaatt atttcagttt ggtttctttg tacactacgt gtttgcgggc
    25381 aaccggatcg aattttttga tttccagttt gccgggcata gtgcgtttat ttttggtagt
    25441 ggtgtagaag tggccagtac ctgcaccgga ttccagtttg attttatcgc gcattgcagt
    25501 aatccttaat taaatagtgt ttaaattaag cttcgccgcg agcacgcaaa tcagccaata
    25561 cgacatcaat gcctactttg tcgatggtac gcaatgcagc gttggaaacg cgcaggcgaa
    25621 cccagcggtt ttcactttct acccaaaaac gacgtgattg caagttgggc aaaaaacggc
    25681 gtttggtttt gttgttggcg tgcgatacgt tgttgccgga catcgggcgt ttaccggtca
    25741 ctttgcaaac tcgtgccata atcagtctcc aaacttctaa aatagtaaaa cgcgatttat
    25801 accacgaaaa acaatgccgt ttcaagtgtt ttaaagaaaa acagatgttt tgcgcgggta
    25861 agccgcccga gtgccgcatc tgcggaacgt ttgcaggtgc gaaacagatg gggcgcgatt
    25921 atagcggatt tcgggagctt ctgcattcaa aatgccgtcc gaacatattt ccagtatttt
    25981 cagacggcat ttcaagtttt caaatccctc aatgtccacc gccgccgctg tggaacggcg
    26041 gtttcgccag ccagacaatg ggaatcagga cgataaacaa aatgctgccg gcgaggaata
    26101 tttcgttcga tccgatgatg aagccctgtt gcgtaatggt attgttgatg atgcctagag
    26161 tttgaccgtc ggaaatgcct tgttgggaca aatgcgccgc cgtttcgtgc aatgttgcgg
    26221 aatagggcgt gatgtgttcg gcaaaacgcg tgtggtgcaa cgcttcgcgc cgttcccaca
    26281 gggtgctgac gacggatacg ccgacaccgc ccatcagcac gcgcaggaaa ttcgacaggc
    26341 tgccggcggc ggcaatctgt ccgcccttca tgtgcgacag tgtgatggtg gtcagcggca
    26401 ggaagaacat ggcaacgcct accccctgcc aaaactgcgg ccagatgacg ttgccgatat
    26461 ccatgtcggc ataaaaatca gtgcgccaat aaaaagtgaa ggcaaaggtc aggaagctgg
    26521 cggttacgag caggcgcata tcgattttat tgccgaacct gccgattaac ggagatagga
    26581 agacggggag gatgccgacg ggcgcggcag caagccctgc ccacgcggag gtatagccca
    26641 agttggtctg caacactaac ggcagcaggg tcagcgtccc catatacacc atgaaaccca
    26701 acgaggtggc aattacgccg acggtaaaat tccgatcttt aaacagcgat aaatcgacaa
    26761 tcggatattt ttctcccaat tcccaaacaa taaaatacga caagcacacc agtgcgacta
    26821 cgcccaaggt aatgatttct ccagaggcga accagtcaag ttccttaccc ctgtccagca
    26881 tcatctgtaa cgcgccgata ccgaccatca tcagcgtcag cccgacatag tcggtcggcg
    26941 ttctgacggt tgccgtttcg cggtgtttca aatgtttcca tgtaatccat gccgatatga
    27001 tcccgatggg gatattaatg aagaaaatcc aaccccaatg ccagtttccg gaaatccaac
    27061 cgccgagtat cggcccgagg acaggggcga caacgacggt cattgcccac aatgccagtg
    27121 ccagcatccg ttttgcgggc ggataggatg ccattaacag gctttgcgac aggggaatca
    27181 acggcccggc gataaagccc tgcaaaatgc ggaaaacaac cagcgactga aggttgggcg
    27241 cgatgccgca cagccacgat gcgatgacga aaccggcggc ggcggcggta aacaatttga
    27301 cctcgccgat acgttttgcc aaaaagcccg tcagcggcac ggaaacggcg tttgccacgg
    27361 aaaaagaagt gatgacccac gtcccctgcg tggttgccgc accgaggttg ccggcaatga
    27421 cggggacggc gacgttggcg atggtcgtat ctaaaacctc cataaatacg gcaagcccca
    27481 aagacagcgt aacccacgcc aatgccgcac ccttaagcgg tggataatcc attgtgtcct
    27541 ccgtttcaga cggcatcagg gcgcggattg cccgaggatt tcatcgaccg tccgatcgac
    27601 ttccgaccag tcggtacttt ccatttccgg caatgccgca cccggcgttt ttgaaacagg
    27661 cgcgcctgcg gcggaagtat ccactttaac cgtcatcgac aaaccgatac gcaacggatg
    27721 cctgtccaca tcttcgcggt tcagcacgat acggacgggg acgcgctgca ccactttaat
    27781 ccagttgccc gttgcgtttt gcgccggaat cagcgaaaac gcgctgcccg tacctgccga
    27841 aaaccctgcc accctgccgc gataaacaat ttgtttgccg tacaaatcgg acaccagctc
    27901 ggcaggctgt ccgattttca tatgccgcaa ctgcgtctct ttaaaattgg catccaccca
    27961 cacatccgac agcggcacca ccgccatcag cggcgcgcct gccgccacct gctgcccgac
    28021 ctgcaccgaa cgcttcgcca cctgaccgtc cgccggcgcg cggacttgcg tccgccgaag
    28081 gttcagccac gcatctttca acctgccgat tgccgtctga acctccggct gttcgcgcaa
    28141 agaaacatct ccgcccaaag ccgcgcgtgc cgaagattct tccgccaaag ccgccttaac
    28201 cgccgcctgc gcctgagaca ctgccgtacg ggcgtgtgcc agctcttcgg cggacaccgc
    28261 acccgattcc gccaaagcgg accggcggcg caaatcatcc tgcgcgcgcg ccaaatccgc
    28321 acggcgcaag gcaacctgcg cccccgcctg cgaagtggcg gcattttgcc ggcggttttg
    28381 gcgcaccgcc tgaaccaact cgttttttgc ccgctcgtaa gccagcacat cattatcgtc
    28441 atccaacacc gccagcacgt cgcctttttt cacggcatcc gtatcgtcgt gcaaaacctt
    28501 ccgcaccgtg ccgccctttt gcggcgtaac ctgaaccacg cgtccggcaa cataagcgtc
    28561 ttccgtttcc tcttcgtgct gccaccataa aaaaaacgcc gatccggcgg ctgcggcgga
    28621 aagcgcgaac agcagcgtca atgccgtcag gcggcgtttg cgtttgactt gcgtgttttg
    28681 aagttttgtt tcgtctgtgt gcgtttccat aagattgggt tccgaatttc ctgatttcat
    28741 ataaacaatg ttctttcaat aggaaataga ttttatatcc gcgcaataat tcaatcaagt
    28801 cagtatttta gatgcaatat tcaaaaaaca acgcttcagg cggcaaaact gaaaatatat
    28861 ttcgtttata tatgacatgg caaacaccgc cgcccgcacc atgccgtctg aagaaaacta
    28921 cacaaatacc gccgcttata ttacaatcgc cgccccgtgg ttcgaaaacc tcccacacta
    28981 aaaaactaag gaaaccctat gtcccgcaac aacgaagagc tgcaaggcat ctctcttttg
    29041 ggtaatcaaa aaacccaata tccgaccggc tacgcgcctg aaattctcga agcattcgac
    29101 aacaaacatc ccgacaacga ctatttcgtc aaattcgtct gccccgaatt caccagcctc
    29161 tgcccgatga ccgggcagcc cgacttcgcc accatcgtca tccgctatat cccgcacatc
    29221 aaaatggtgg aaagcaaatc cctgaaactc tacctcttca gcttccgcaa ccacggcgat
    29281 tttcatgaag actgcgtcaa catcatcatg aaagacctca tcgccctgat ggatccgaaa
    29341 tacatcgaag tgttcggcga gttcacaccg cgcggcggca tcgccgttca tccgtttgcc
    29401 aactacggca aagcaggcac agagtttgaa gcattggcac gcaaacgcct gttcgagcac
    29461 gacgcacaat aaaccatcct tcccatattt cagacggcat tccttcgata cggtatgccg
    29521 tctgaacgcc tttccgaaag gtttttatgt acgcattgac cgccgcacag caacagaagg
    29581 cactcttccg gctggtgctt ttccatatcc tcatcatcgc cgccagcaac tatctggtgc
    29641 agttcccctt ccggattttc ggcatccaca ccacttgggg cgcgttttcc tttcccttca
    29701 tcttcctcgc caccgacctg accgtccgca ttttcggttc gcacttggcg cggcggatta
    29761 tcttttgggt gatgttcccc gcccttttgc tttcctacgt cttttccgtt ttgttccaca
    29821 acggcagttg gacgggcttg ggcgcgctgt cccaattcaa cacctttgtc ggacgcatcg
    29881 cgctggcaag ttttgccgcc tacgcgctcg gacaaatcct tgatattttc gtattcgaca
    29941 aattacgccg tctgaaagcg tggtggattg ccccggccgc atcaaccgtc atcggcaatg
    30001 cactggacac gttagtattt tttgccgttg ccttttacgc aagcagcgat gaatttatgg
    30061 cggcaaactg gcagggcatc gcttttgtcg attacctgtt caaacttacc gtctgcaccc
    30121 tcttcttcct gcccgcctac ggcgtgatac tgaatctgct gacgaaaaaa ctgacggccc
    30181 tgcaaaccaa acaggcgcaa gaccgccccg tgccctcgct gcaaaatccg taaaacagct
    30241 ctgtagggaa tcgggacggt tgggttttct tttatttgca aacactgccg gaacggataa
    30301 atccggattc ccacccactt cgtcattccc gcgaaagcgg gaatccggtg cgttggggtt
    30361 cagtcatttc cgataaactc ctgccgcatt gtatttctag attcccgctt tcgcgggaat
    30421 gacggcggat gggtttccgt gccgtggtgt ccggattccc gccggtacgg gaatgacggc
    30481 agcgcttcct tggcttagtt ttattttaag ccactataaa tgccgtctga aaacctgttt
    30541 gcagattttc agacggcatt gttttcgtcg tcggttcaaa cttcaatccg attgcgacac
    30601 ggtaaccggt atgccgcgca agcgcgcaag cagcgcgtcc gccttctgtt tctgcgcggc
    30661 aaacgccgcc ttgtccgcct gcgtcaattc gggtgtcggc aatgcgaccg aaacaggatt
    30721 gacgggctgc ccgttgatgc gcgcctcgta atgcaggtgc ggcccggtcg aacgccctgt
    30781 cgaaccgaca aaaccgatga cctcgccgcc gcgcacattg ccttgtgcct gcgaaaacgc
    30841 gctcaagtgc gcgtacagcg tttccacacc gttggagtgg cgtatcatca ccgcgttgcc
    30901 gtatccgccc ttccggcctt taaaggtaat cacgccgtcg gcggaagccc tgaccggcgt
    30961 tccctgcggt gcggcataat cgatgcccgt gtgcagccgc catgtgtgca ggatggggtg
    31021 catacggtag ccgaacggcg aagaaatgcg cgtatagacc agcggctcga tgttgaagcc
    31081 gcctttttcc tgcaacaccc tgccgtcttc atcgtaataa ttgccgcccc ctccgccttc
    31141 cttgtccgaa cggtaataga acgcctgatg ggttgtgtcg cccttgacaa cttccgccgc
    31201 caaaatatcg cccgccgcca cctgctgccc gtggaaatac aggctgtcgt aaagcaggcg
    31261 cacggcatcg ccttccttca aaccgtcaag gctgaagcgg ccggcaaaaa tcccgcttaa
    31321 ggattcgcgg atttcgacgg gcacttccgc ccgcgccagc gaaccgcgcg ccgacgtttt
    31381 gaccacgacc gaacgcagtg tcggcaaaac cttcatatcc gcatcagaag ccgaccgccg
    31441 ccatatgccg cctttttttt ccaaagcgac cagattgcgc tcgccgtctt cgtcggtaaa
    31501 aaactgcact tcgcgcgcac tgccgtcgcc gccgaccaaa acatgaaccg actggtcggc
    31561 acgcaaatgc cgcaaatcgg cttcgccgcc atatttttcc gtgattcggg caatctcgtc
    31621 ccgcgccata cccgaacgcg ccagcacgtc cgccagcgag tcgcccggct gcaccgcctc
    31681 ctgcacccaa tatgccgtct gaacgccgct gccgccccaa gacagcggcg gcagtttttg
    31741 ttccacgcgc tgcggtctga cgcgctccgt cccctctgtc gaagcaatgt atgccgccga
    31801 caccagaata atcgaaacgg caagcgcgcg cagcgcgtat ttccgatgtt ttgccgaaag
    31861 tgggaagaca gccatattgt tcctttttga cggcgtttgc gccgaatcct ctataatatc
    31921 cgcccgatcc tcatttgtca ggcatcaaac gatgaaactc accctcgccc tcccctcgct
    31981 caatttggat gaagacgaaa tccgaatacc tctatgcctg cccgccttca acaaaatcct
    32041 gcaatacggc tcgccgcacc gccaaagctg cacggcttcg gcattttacg cacgttattt
    32101 gtggtgcgga agactggcac aacgcccggc gcaaagcctg aatatgccgt ctgaaaccgt
    32161 cgcgctcgcc acgcccgtct ggcaaaaaat ggggctgcat caggcaaatg tgctgacggc
    32221 ggaatatttg gatgtcggta cggatgaagc ggaacggctc tgccgcgatc tgtcggcgtt
    32281 ttacggcgac attccgtggc gtttcgttcc cgtcctgccc gaattatggc tggtttccct
    32341 tccccgcgcg taccgctggg gcgcgaagcc cgttttggac ttgggcggac tgttgggcgc
    32401 ggacgatcag ccggacgggg aagacgcgtt ggaatggctg cgcgtccaaa ccgaaatcca
    32461 aatgtggctg aacgctcacc ccgtcaacca caaccgcaaa aaacgcgggc tgcccgaatt
    32521 gaacggctta tggctttggg acagtctgca cggaagcgca caaggcggca cgcttttcgc
    32581 cgatacggtt tggagccgtt ttcacccgaa ccgccgagca ttgcccgaca gcttccgagc
    32641 ttatgcggaa acggcggcac acctgcccga tacgcaccac atcctgttta tggacgattt
    32701 gcgcctgacc gccctgacag gggacaggga gcggtatgcg gcaatattgc agcagtggga
    32761 agaacgctgg ttcgcaccgc tttacgaagc cgtccgcacc ggcaaaatca aacggctgga
    32821 catcgccacg gacggacaac acggcggtac gctgaccttc aaacccacag acaggcggaa
    32881 gttttggcgc tgcacgaaaa cctttgacgg aatctggtaa acccgcccgc cgcaaccgcg
    32941 acaatgccgt ctgaagcccg aatcgggttt cagacggcat tttaccccgc atcgccgtta
    33001 tgccggaaaa accggaatac gtttccgcac acgcggcggc acgcaaaaca ccgcccgaaa
    33061 gccgccgatg cccgccgtga caccgccgcc ggcagggttt ccgccgaaaa ggaacagcaa
    33121 tcagaacgtt ttgttaaact cgacaaacgc cctgtttttc tcgtattcgt taaacacatc
    33181 gttgctccac gtttcgcggt gcgacagcgt caggcgcggc gtgatgcctt tgaaatgcaa
    33241 tgcccggtgc caaaggctca aggatgtgtc cgattcttta tccctgcgcc tttccccttt
    33301 aaaactgctg aagaagccgg gtttttcata atgccgtttc gccacgccga ggcggaacag
    33361 cgaagacagg ccgctgccgc cccattcctg cccccaggca aagcgcaggc cgtaacggtt
    33421 gaaattgtcg ccacggtcgg cggggttgcg ctcgcggtaa aaatccaaac cgcccgtcca
    33481 atattggcgc gcattccggt aaaacaccag cgaattggaa atttgcaaat gggtattgtc
    33541 ggaacgcgcc cggcgcgtat tcttcaaacg cccccactcc gccgaagaca gcgtttgcca
    33601 tctcggggtt tgccaacggt tgaaataaag gcgtgcgccg ttggcgtaag aataagcgtc
    33661 gttgccgtag gtgcggcgtt cgtggaacac tgccagcccg acatctttac gccggtcggc
    33721 aaaaccgatg ccgccggaaa cacctgccgt catatcgttg aatttcttat tccccggata
    33781 aaccctgccg gacacgtcgc cgcccgccgt cgtgtaccag ccgtttttca gcgaccattt
    33841 tttctccgcg ccgaaccggt aattgaccgc cgtgccgtcc acctgtttcg ggaaagtcca
    33901 attgccgtac tgctgctgtt tcggggcttg gttgatattg tgttcgcggg taacgctgaa
    33961 accgccgttt accttccacg catcgcgttc gcgcaatgcc ttgcggtaca gctcgacctg
    34021 ctccataagc tgcggcggca gatcttctgt tttcaggcgg tcgaactggt ctgccgccgc
    34081 ctcgttctgc ctgtcttcaa acaatgccgc cgccaaacgc atacggacgg cgggcgcgtc
    34141 gggttgggcg gcaatcaatt cccggtaatg ggaaaccgcc tccttcaccc tgccctctgc
    34201 ctgcgccaaa atcccttgtg cataaagtgc caacatctta tcctgccgcg cctgttgtag
    34261 gtaaatcggc aaaatcacgc ggataccggc aatattgttt gagaccactg cggaatacat
    34321 ggcacgcgac aacaattcgg gatttttcag cagggtttcg ccgtcaacct gcaaaacttt
    34381 tcctttttcc cgcacctgcc ccggtacctt ctccctgtcg atcggtttga cctccgcttc
    34441 atgaagcctg aactcgggac ggcttctcaa atccggttcg cgcggtgttt cttcggcgta
    34501 tgccgcgctt gccaataaag gcagcagcat ccatctgttt cgtgcaggca taaatgtctt
    34561 cccacaaaaa taaaaataaa aaataaccat tatcaaccgg cggattgtat cagtttttta
    34621 tgccgctgtt ccaaaccctg ccgcccgatg ccgcgacaat gccgtctgaa gccagaatcg
    34681 ggtttcagac ggcggcaaaa aaccggcggt tttccgccgg tttcatcatt atgccccgcc
    34741 cttctgggcg gcagggttcg gatcggtttg cggggcggcg cacggggccg tgcaggtgtg
    34801 ccgcctgtgt gtcaaaacat ttcccggcgc atcaaaacaa ccatttgacc gacaatgcgg
    34861 cttctttgtc gccgtccgtc cttttgccgt atccgatgcg tgcggacata tggcctttcc
    34921 aaccggcttc aatgccaaac cgcccttcga gcgccgtcct gcctgccagc gtctgttttt
    34981 cgccgtccat ttccatgccg aaagattttg acctgtgcaa aacattaaaa gcggcaaaag
    35041 gctgaagatt gacaccgtta cgcaaagcaa aacgggtttt tgcccgaatg ccggcgcgga
    35101 tttgccactg accgctgccg agcagcccga ccgccgcccc tcgctgtcgg taaagccgcc
    35161 gtttacgccc aagtaggtaa actgcgcctg cggttgcagg taaaactgca tattactgcc
    35221 ttatatattg cgtccctaag aaggggcgat taacaaaaat taacgcccct tcttagggac
    35281 gcaatatata aggagtgagt acctgttctt ctattaaaga attaatcttt tgaggcattt
    35341 tctgttgaag tttattaaac acatcttcag aaattccaaa ctgctttatt atctcttccc
    35401 taacccgttt tttattttct tcccaagcct cgggtcttgt tttgtataaa tcctgtaatt
    35461 gctttttaac tgcctgcttt aaatcagttc ggtaattatc tgtaatattg atttcgtcat
    35521 acaaggcttc agatgatttt tgatcggttt taccgctgga gaaataatgg aaaacaccgt
    35581 ttgccggctt gtctgaaaat gaaaaatcgg cagtatcgcc aacatttgta ggacgagtat
    35641 ccgaaccggg ctgataactg aaagcaccga agcaatcata aagatatgct gcacgggccg
    35701 cgccaaacgt gggaatttct gtcttccgcc gcttctaaaa taaatttgtt tttaatctcg
    35761 gcttctttga cgggatcgta cgcatccgaa ccccaagcat atggcgtgca aaacagcgat
    35821 aaaacagata atgttaatag attaacccga ccgcttgctt tcagtgtcat gagaaatttt
    35881 cctttgtcaa gtgttaaagt tataatgatt atatactata tcacactaca tcgcaatgaa
    35941 aaatcgggaa aaacaaaaaa cccctccgcc gtcattcccg cgaaagcggg aatccagaat
    36001 ttcaacgcct caagaattta tcggggatgg ccggggttta aaggtctggg tttccgcctg
    36061 cgcgggaatg acggtttaga aatcacccga aacgagaaaa agaaccgaaa ccgaacaagc
    36121 cggattaccg cctgcgcggg aatgacggct caaaagttac gaaacgaaaa ataaccgaaa
    36181 ccaaacaagt cggattcccg cgcgggcggg aatgacgata agcgtaattt caatctgcca
    36241 tattgtcttt tcacttgcct gaaaacagat ttcagccacc tatccctgca tccggcggct
    36301 gatgtagtcg gcaatcaaat cccgcttttc ccaaaaaaac ttctgcattt tttgttcgga
    36361 atacagtttg cggaccagag tgggcaaacc gctgtggacg atggcggcga tttctttgtt
    36421 gccttcttct tcctgcattg ccgtctgaaa atccataccc ctgcctttca gatattctgc
    36481 aaggcggttg cggatgtggg aattgagttc gatggatttg agcattttgc ctccgttttg
    36541 cggtaaggat ttattcgatt tgtttatccg cctgatttta tcacgtttcc caagcggttt
    36601 gcctgtacaa taccgctttt cggcagggtg tgggcgatga tgttttcttg gttcaagctg
    36661 tttcacttgt tttttgtcat ttcgtggttt gcagggctgt tttacctgcc gaggattttc
    36721 gtcaatatgg cgatgattga tgtgccgcgc ggcaatcccg agtatgtgcg tctgtcgggc
    36781 atggcggtgc ggctgtaccg ttttatgtcg ccgttgggct tcggcgcggt cgtgttcggc
    36841 gcggcgatac cgtttgccgc cggccggtgg ggcagcggct gggtacacgt caaactgtgt
    36901 ttgggcttga tgctcttggc ttaccagttg tattgcggcg tgctgctgcg ccgttttcag
    36961 gattacagca atgctttttc acaccgctgg taccgcgtgt tcaacgaaat ccccgtgctg
    37021 ctgatggttg ccgcgctgta tctggtcgtg ttcaaaccgt tttgacggag catcttatgc
    37081 cgatagaaat cgaacgccgt tttttgattg gaaacgacaa ctggcggcaa tacgccgacg
    37141 aaccgctgct gttgaggcag ggttatctgt ctgttgaaaa agaacgcacc gtccgtgtcc
    37201 gcattgccgg aaaacaggcg tggctgacgc tgaaaggcta tatttcggaa atcagccgca
    37261 gtgagtttga atacgaaatc ccgcttgccg atgcggaaaa gatgatggaa acgatgtgcc
    37321 cgtttaaaat ggaaaaacgg cgttatccgg tcagatgggg cggcagcctg tttgaaatcg
    37381 atgtttttct tggcgataat gcgcctttgg tcgtcgcgga aatcgagttg cccgacgaga
    37441 atgccgattt tgaccgtccg gactggctgg gacgggaaat cactgccgac ggtatgttta
    37501 ccaatgctta tttgagcagg catccgttct caagctggaa aaatgccgtc tgaaacggct
    37561 tcagacggca tctgccttgc ggttatccct tgaggtaatg cacgaatgta tatgcaacgc
    37621 ctttgctgct gacacggcgt tccgtccgct ctgcttcttt ccaatgcgtc cggtctattt
    37681 ctgggaaaaa tgcatctcct tccacagaca aatccacttc ggttatccgc aaatcggtcg
    37741 ctaatggcat cgcttgtccg tatatctgcg cgccgcccat aatgacggct tcttccgcgc
    37801 cggcgcacaa tgccaatgcc gcctccaaac ttgccaccgt ttccgcgcct gccgcgcaat
    37861 aatccgcctg ccggctgatg acgatgttcc tccgtccggg caggggtttg acgggcaggg
    37921 attcccacgt tttccgcccc ataatgacgg gtttgcccaa ggtataggct ttgaaaaatg
    37981 cgaaatcttc ggggatgtgc caaggcatag cattgcccgc cccgatgcac aggttttccg
    38041 cacacgccgc aattatggtt attttgagca tattttctcc acaaaaatgc cgtctgaacg
    38101 cttcagacgg cattttatga cattctgcgt caaaaatctc aacctgcgcg cgccatacgt
    38161 tttgtgttcg cacccgccaa caatgccaac aattcttcag tcgcgcccca accgatacac
    38221 gcatcggtaa tgctcttgcc gtacacttcc ggcttgtcct gcctgccttc gaccaaatgg
    38281 ctttccacca tcacgcccat gatattgccg ccgtcctgtt ccaattggtc ggcaatgtct
    38341 tgtgccactt ccatctgccg ggtgtaatcc ttgcggctgt tggcgtggct gcaatcgatc
    38401 atcagcttgt cggttacccc tgccgcacgc agttgttcca ccgcctcgct gacgtgtccc
    38461 gcatcataat tcggctcttt tccgccgcgc aaaatgacat gacagtcggg attgccgccg
    38521 gtatggacaa tggcggaatg cccggccttg gttacagaca agaaatgatg cgaatggctc
    38581 gccgcaccga ttgcgtcgat cgcaatcttc aaattgccgt ccgtaccgtt tttaaagccg
    38641 acggggcagg acagaccgct tgccaattcg cggtggactt ggctttcggt cgtccgcgcg
    38701 ccgattgccc cccaagaaat caggtcggca taatactggg gtgtaatcat atccaaaaat
    38761 tcggtagagg caggcatgcc catattattc agggacaaca acaggctgcg cgcctgacgc
    38821 aaaccgaaat tgatgtcgaa cgtaccgtcc aaatgcgggt cgttaatcaa acctttccaa
    38881 cccaccgtcg ttctcggctt ctcgaaataa acgcgcatca cgatcaaaag ctcgttttca
    38941 taccgcttgc ggagtttcaa cagacgttcc gcatattcca acgccgcttt cggatcgtga
    39001 atcgaacacg gcccgataat gaccaacagc cgcctgtccc tgccgtgaac caaatcggaa
    39061 atttcctgac gggtgcggtg aaccaagccc gaagcctctt tggaaatcgg cagctcgtaa
    39121 agatgggcaa tcggcggcaa caactcttta acttctttaa tcttaatatc gtctgtgggg
    39181 taatggtgtg tcatcttgca tctttcctga tgggttttta tacgcggctt aggttacatc
    39241 gtttaccttg cacaaacaag tgcaacatat tactacaaag ctattttatt tcaaaataac
    39301 tgatatatct tttttcatag attttaaatt tcatattttt aatattttag gaattttatt
    39361 tcaaagaaaa agaaacagat tgataaaaac aatacccgcc attcattcgg gcaaaaccgc
    39421 acggaacatc cgttttcccg tttccacgac aatccggtac aggacacccc gctgccgatt
    39481 ccgcctgcac atccggcaaa atttcatacg caaggaaaat gttgaaaaca aaaagaacta
    39541 attgcttgaa aaaagattta attggaatgc gggtatttcc cacactttct ccatttattt
    39601 atcctcatta taaaaacatg aaaaaaaacc cctgcttgca ctctcacttg cagccgtgtc
    39661 cgccgttacg gtagccgata ttcccgagtc taacggcaaa ggcgaataca aagtctttac
    39721 cgcttatgtt aaaggcaaag ccactgccgg caatttgaaa gaaaaataac gcacaccgtc
    39781 catcggtttt cgttcaacga acgccacgat atgcgtttta aggcagagta cgcccgaagc
    39841 aaaaacgatg cagacgacaa aagagggaat gcacacatcg ctaacggcgc aataggcaaa
    39901 atggctgccg tgtatgccgg ttatacctac acccaaccgg gcggcgaatc cacacgtttc
    39961 cgtggcggtt tggagcgtgt ccaagttaaa cgttatctgg gagaaaaatg ctgcggctac
    40021 gcctgtccaa aaagcgaacg cttcaccctg caagcagatg gtgaaatccg tcaaaacctt
    40081 ggcgaaggct ggtttctgaa gctatgagcg gggtggctgt ctatctccgc aacaaaaaca
    40141 aaatagcaat tgcctcccaa gatgccaacc taaactccaa aggccggttc gtcagcagcg
    40201 gtctgaatgt gggcaaacaa ctgaccggca gtctcggcgt agagtttgac ccatactacc
    40261 gccaccgcgc aatccgcaag tctgccgaat ttgtcccgaa cactaccaaa accaaaacag
    40321 acagcgaaaa gtttaacgag tagggcttcc gcgtagccgc aacgttctaa ttcccttacg
    40381 tataaaaagc agcataaaaa tgctgccttt tcccatttcc aagggaagta ttccgtagtg
    40441 cagcttacct cattgtttca cacaaaccga aaatgccgtc ttaaaaccaa ttttcagacg
    40501 gcatttgtcc cttaaaacac gttttttcaa gcgcgaccat accaaacacc cacaggttgc
    40561 cttccaccct tcgtacaacc tttgctccat caaacatcca cttcctaaat actgatgtat
    40621 actg
//
LOCUS       DBJGGI010000010        40360 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.010, whole genome shotgun sequence.
ACCESSION   DBJGGI010000010 DBJGGI010000000
VERSION     DBJGGI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40360
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.010"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            241..2485
                     /locus_tag="ACSIGJ_000557"
                     /pseudo
     CDS             241..2485
                     /locus_tag="ACSIGJ_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            2610..4160
                     /locus_tag="ACSIGJ_000558"
     CDS             2610..4160
                     /locus_tag="ACSIGJ_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HHQ1329511.1"
                     /translation="MKKPLISVAAVLLGVALGTPYYLGVKAEESLTQQQKILQKTGFL
                     TVESHQYDRGWFTSTETTVIRLKPELLHNAQKYLPDNLKIVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGETVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFIDSEGRFRFDTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGDASGLFTHDPVLNIKIFRFTLPQGKIDVGGKIMFK
                     GMKKEDLNQLGLMLKKTEANIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEARASIAD
                     INETLRLMVDSTVQSMAREKYLTLDGNQIDTVISLKNNALKLNGKTLQNEPDPDFDEG
                     DMVSGQPH"
     gene            complement(4584..6368)
                     /gene="lpdA"
                     /locus_tag="ACSIGJ_000559"
     CDS             complement(4584..6368)
                     /gene="lpdA"
                     /locus_tag="ACSIGJ_000559"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HHQ1329512.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVIKEVKVKVGDKISEGGLIVVVEAEGAAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGSAADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAAPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGVCTDLPPQKKK"
     gene            complement(6386..6820)
                     /locus_tag="ACSIGJ_000560"
     CDS             complement(6386..6820)
                     /locus_tag="ACSIGJ_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329513.1"
                     /translation="MSYLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKAT
                     HGPSVKNAVYAHQIETDLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQY
                     SIFEYEVEHPENPDEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(6921..8483)
                     /gene="aceF"
                     /locus_tag="ACSIGJ_000561"
     CDS             complement(6921..8483)
                     /gene="aceF"
                     /locus_tag="ACSIGJ_000561"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HHQ1329514.1"
                     /translation="MSIVEIKVPDIGGHESVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAAEAQPAPAAAGG
                     ATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVVKAV
                     FLKVGDKVSEGSAIIEVETAGSAAAAPAPAAQAAAPAAVPTSASPAAAKIDEAAFAKA
                     HAGPSARKLARELGVDLGQVKGSGLKGRIMGDDIKAFVKSVMQGGAAKPAAAGASLGG
                     GLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHEEADMTELEEF
                     RKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKNYFNIGFAADT
                     PNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTISSLGGIGGTG
                     FTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVIDGAAGMRFTVFL
                     ANLLKDFRRITL"
     gene            complement(8633..11296)
                     /gene="aceE"
                     /locus_tag="ACSIGJ_000562"
     CDS             complement(8633..11296)
                     /gene="aceE"
                     /locus_tag="ACSIGJ_000562"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HHQ1329515.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLLKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSDFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAIETERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            11995..13707
                     /locus_tag="ACSIGJ_000563"
     CDS             11995..13707
                     /locus_tag="ACSIGJ_000563"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HHQ1329516.1"
                     /translation="MKASQFFISTLKEAPAEAAFASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHER
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQATYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGELVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGLVGFTGKVYADFATEKGADWVIGANEDDYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRGKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADRIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRDNEAQAVAIGEIVARVTA
                     SLNA"
     gene            13770..14426
                     /locus_tag="ACSIGJ_000564"
     CDS             13770..14426
                     /locus_tag="ACSIGJ_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HHQ1329517.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHVEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLIGRDSDGTGEGEDILGCVAGYGVGLDLTA
                     RDIQCRLKAQGLPWLKAKGFRHSACVSDFAAADRIGNQDKVLFSLKQNGVLKQRGETG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVCASFTIGC
                     "
     gene            complement(14436..14891)
                     /gene="ruvX"
                     /locus_tag="ACSIGJ_000565"
     CDS             complement(14436..14891)
                     /gene="ruvX"
                     /locus_tag="ACSIGJ_000565"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HHQ1329518.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLATVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHADGTEHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVLGKKRKSVLDQVAAQAILHGFLEGGPAECFNGREG"
     gene            complement(14884..15432)
                     /locus_tag="ACSIGJ_000566"
     CDS             complement(14884..15432)
                     /locus_tag="ACSIGJ_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HHQ1329519.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDGIALTS
                     SRDVLENISREGAVDKALISIGYSSWSKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(15434..15994)
                     /locus_tag="ACSIGJ_000567"
     CDS             complement(15434..15994)
                     /locus_tag="ACSIGJ_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HHQ1329520.1"
                     /translation="MNRLLLLSAAVLPTACGSGETDKIGRASTVFNMLGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKRGTGFAFKSRQIVRYYDPKRKAFAYLVYSDKIVQGSPKNSLSAVSCFGSGIPQTDG
                     VQADTSGKLLAGACIISNPIKNPDKR"
     gene            16321..18117
                     /locus_tag="ACSIGJ_000568"
     CDS             16321..18117
                     /locus_tag="ACSIGJ_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HHQ1329521.1"
                     /translation="MQYKPLLLALMLVFSAPAVAAHDAAHNRSAEVKKQAKNKKEQPE
                     AAEGKKEKGKNAAVKDKKTGGKEAAKEFKKTAKNRKEAEKEATSRQSARKGREGDKES
                     KAEHKKAHGKPVSGSKEKNAKTQPENKQGKKGAKGQGNPRKGGKAEKDTVSANKKARS
                     DKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVSNSL
                     KQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEIAATKA
                     QISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQKAL
                     AVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKISKDARKLLEQKG
                     NEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRAEMS
                     NLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGVFYS
                     TAPATVESIAPGTVSYADELDGYGKVVVIDHGENYISIYAGLSEISAGKGYTVAAGSK
                     IGTSGSLPDGEEGLYLQIRYQGQVLNPSGWIR"
     gene            18244..19719
                     /locus_tag="ACSIGJ_000569"
     CDS             18244..19719
                     /locus_tag="ACSIGJ_000569"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HHQ1329522.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDGRDNEVLPVQ
                     SIRTMAEVYGQIKANYYHDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAEVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDGMVLKAVPEDYVYGMGGDPLAGIPAELKTIPMAVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERT
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAAKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKK"
     gene            20049..22076
                     /gene="uvrB"
                     /locus_tag="ACSIGJ_000570"
     CDS             20049..22076
                     /gene="uvrB"
                     /locus_tag="ACSIGJ_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HHQ1329523.1"
                     /translation="MEVIRYPNSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLHQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDALRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAVDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRNIKENLLFGAE"
     gene            complement(22254..23012)
                     /gene="cah"
                     /locus_tag="ACSIGJ_000571"
     CDS             complement(22254..23012)
                     /gene="cah"
                     /locus_tag="ACSIGJ_000571"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HHQ1329524.1"
                     /translation="MPRFPRTLPRLTAVLLLACTAFSAAAHGNHTHWGYTGHDSPESW
                     GNLSEEFRLCSTGKNQSPVNITETVSGKLPAIKVNYKPSMVDVENNGHTIQVNYPEGG
                     NTLTVNGRTYTLKQFHFHVPSENQIKGRTFPMEAHFVHLDENKQPLVLAVLYEAGKTN
                     GRLSSIWNVMPMTAGKVKLNQPFDASTLLPKRLKYYRFAGSLTTPPCTEGVSWLVLKT
                     YDHIDQAQAEKFTRAVGSENNRPVQPLNARVVIE"
     gene            complement(23421..23768)
                     /locus_tag="ACSIGJ_000572"
     CDS             complement(23421..23768)
                     /locus_tag="ACSIGJ_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329525.1"
                     /translation="MTYLKVIAISIVLYILLLQINLKMLEKRIDFLVENIDKYYQQYG
                     SYPNNFDFISTKTDFTTESYCDFWDKNIAGYGNCYFVKNDKDYTILVMGFSSKILFSS
                     HNKIKEFNSNKYD"
     gene            complement(24092..24808)
                     /gene="trmB"
                     /locus_tag="ACSIGJ_000573"
     CDS             complement(24092..24808)
                     /gene="trmB"
                     /locus_tag="ACSIGJ_000573"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HHQ1329526.1"
                     /translation="MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGMASAEIARRLPETDFLAIDVHGPGVG
                     NLLKLINENHLENIRVMRHDAVEVVKNMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(24930..26334)
                     /locus_tag="ACSIGJ_000574"
                     /pseudo
     CDS             complement(24930..26334)
                     /locus_tag="ACSIGJ_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            complement(26451..28337)
                     /gene="uvrC"
                     /locus_tag="ACSIGJ_000575"
     CDS             complement(26451..28337)
                     /gene="uvrC"
                     /locus_tag="ACSIGJ_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HHQ1329527.1"
                     /translation="MPSEAVFQMIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GNVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVRHIETTITRSEAEALILENNFI
                     KALSPKYNILFCDDKSYPYLMLSGHQYPQMAYYRGTLKNPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYCDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIIQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGLVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAITQRHLQQSNQ
                     QHRIDELAKILGMNSDGINRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKIQEAEANGESVKWPDVVLIDGGKGQIGIAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGELFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLGDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(28392..30569)
                     /locus_tag="ACSIGJ_000576"
     CDS             complement(28392..30569)
                     /locus_tag="ACSIGJ_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HHQ1329528.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VTFDGSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLTINIPFLIG
                     MVGMMLKGLNWTRHDWMIPPVWQFVLASIVQLWLAIPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSSHAAHGMAHVYFEAGVMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRNGEWKQLPIDQVQIGDLIRTNHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPTVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGMGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYYVPDSGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLEIPAAQNAQTVVGAGITAEVEGVGLVKS
                     GKAEFAELTLPKFSDGVWEIASAVTVSVNGKPIGAFALSDALKADTAEAIGRLKKHNI
                     DVYIMSGDNQSTVEYVAKQLGIAHAFGNMSPCDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALLISQATLENIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLGNALRLKWVKID"
     gene            30708..31658
                     /gene="trxB"
                     /locus_tag="ACSIGJ_000577"
     CDS             30708..31658
                     /gene="trxB"
                     /locus_tag="ACSIGJ_000577"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HHQ1329529.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFALKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSDQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            31814..32182
                     /gene="rpsF"
                     /locus_tag="ACSIGJ_000578"
     CDS             31814..32182
                     /gene="rpsF"
                     /locus_tag="ACSIGJ_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HHQ1329530.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMITEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVGELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLVGAAEEAAAQ"
     gene            32183..32485
                     /gene="priB"
                     /locus_tag="ACSIGJ_000579"
     CDS             32183..32485
                     /gene="priB"
                     /locus_tag="ACSIGJ_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HHQ1329531.1"
                     /translation="MGFTNLVSLAALIEKAFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIKEYKG"
     gene            32492..32722
                     /gene="rpsR"
                     /locus_tag="ACSIGJ_000580"
     CDS             32492..32722
                     /gene="rpsR"
                     /locus_tag="ACSIGJ_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HHQ1329532.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            32739..33191
                     /gene="rplI"
                     /locus_tag="ACSIGJ_000581"
     CDS             32739..33191
                     /gene="rplI"
                     /locus_tag="ACSIGJ_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HHQ1329533.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARARQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVIAAAE"
     gene            complement(33814..34620)
                     /locus_tag="ACSIGJ_000582"
     CDS             complement(33814..34620)
                     /locus_tag="ACSIGJ_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HHQ1329534.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQTVDWKTIFAMMPGMIFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTGRQTASRPLPGLPGLTAVSTLFGAMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLVNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKESFGIMLLLIAGKMLYNLL"
     gene            complement(34621..35367)
                     /gene="pssA"
                     /locus_tag="ACSIGJ_000583"
     CDS             complement(34621..35367)
                     /gene="pssA"
                     /locus_tag="ACSIGJ_000583"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HHQ1329535.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            35950..36432
                     /locus_tag="ACSIGJ_000584"
     CDS             35950..36432
                     /locus_tag="ACSIGJ_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HHQ1329536.1"
                     /translation="MMKKILAVSALCLMTAAAQAADTYGYLAVWQNPQDANDVLQVKT
                     TKEDSAKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVSLAYPKALGAMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGAQGQCGLETVYCTSSSYYGGAVRSLIQHLK
                     "
     gene            complement(36479..37690)
                     /locus_tag="ACSIGJ_000585"
     CDS             complement(36479..37690)
                     /locus_tag="ACSIGJ_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HHQ1329537.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVTGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTTSIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGIKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFVGVGLCAVLAIV
                     LNSLLPDPKES"
     gene            37912..40350
                     /locus_tag="ACSIGJ_000586"
     CDS             37912..40350
                     /locus_tag="ACSIGJ_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HHQ1329538.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTEAKKARPEHVVNLIADALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDA
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALRKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQPSLFEDNEVQQNGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPEEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEQAKAAGKPLLNPFSLNLDEPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGTFVSDGEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGIVSPSD
                     LNGSRKILAHKDHL"
BASE COUNT         9759 a        10039 c        10996 g         9566 t
ORIGIN      
        1 tttcgactta acttcggcac accgtcccgg cagctaaaaa tcctgcggga tcggtgtgga
       61 atttagggat aatctagtac agccacaaaa ataaaccaat atttaacagt cggatagctc
      121 cgcctgatta agtttttcgg taatagttgt gattatcaaa tataattgcc gcccgaaata
      181 tccgataagt ttgtttgttt tatctttaat tccccaatac ttaaaaaaga gagcgttttc
      241 atgaacaaaa aacttgcctt aatgccgctg ttgattttga gcgcgttttc atctgccgcc
      301 gacaatactc cgcagcatgg cgaactcgga caggttcatg tccgcgccga tgccaagcgc
      361 gtcaaagccg cgcattctta ctccatcgcc agcgatggcg acttgcgcga ccgcgtaaat
      421 ttgggcgtat tgggtaaagc caacgcgttt accgcgccga ttaccgtcgt caactacgac
      481 gaacaagcct tcaacaatac cgaggcgcgt actttagtgg atgccgtagc caaaaaagac
      541 gcttccgttt gagagtttgg cggcgaaagc aacacgctga ccggtctgta tttccgtggt
      601 tatcagcttg atgcgcgcca attcagcgta aacggcttgg caggaatgta tggcacgcaa
      661 ggcacggcga gcgtgcaagt cggcttcgca caactgatta aaggcgcgtc caccgccgta
      721 aacggcatac cctgaaggcg cggtatccgg ttccgtcaac atcgaaacca aaaaagccgc
      781 cgacgaaggc aaccgcaaaa tcggtttggc ccggttcggc aacaaccgcg cccaaggtag
      841 gttcgactta gggcaacgct tcggcgaaaa caaagcattc ggcgtgcgtg ccaacggcaa
      901 actgcgccac ggcgacaccc cgcgccacgg ttacagagaa gacaacaaag aatttgcctt
      961 aaatgccgat tatcgcggcg aaaaactgcg cgtgacgttc gactccatct acgcgaaacg
     1021 caaaatcaac ggcggccgcg cgcgtatgca ggatatccaa aacgccggcg gacgcttgtt
     1081 cgacgcgccc gacggcaaaa tcaacctgct gccttcttgg aactggcaaa ataccgtcgg
     1141 cgaaaccaat atgctgacgt ttgaatggga tgcgtttgac aatacctaaa ttaccggcgg
     1201 tatcggctac aacaaagcgc gttattacgg tacgctgatt tccccaaccg tttgcggtac
     1261 aagcggtgcg agcagccaaa ccaaaacctg catggctgcc aaccaatacc acaccggcac
     1321 ggcacgcctg accgaccaat atttccgtac tttgagtatg aacctgaccg cacgcggcga
     1381 atttgaaacc ggcccggtca cgcataactg gagtacggct ttcgaccgcg ttatccgcca
     1441 gcggaaaacc ataaacggca gcgggaacgg caacagcaag atagaagtga aggcaaacga
     1501 aaatattgcg aatcaattgg catcgttcaa agccgactat cccaattctt gggcgaactc
     1561 ggccaacttg gatgccaata tcaaagtcaa cagcttggct ttgtccgata ctttgggctt
     1621 cgttgataac aaataccccc tgactttggg cggacgtttc caagcggttg aatacactga
     1681 taagaagaaa tcacagagcg gcgatgccaa acgtttcagc ccgatgctga tggcggcgtg
     1741 ggtgccgcaa cctgatttgg tcgtgtgcgg caactatatg gaagacttgg agcgtgccga
     1801 tatcaaaacc gatgattccg gcgaaacgac aatggcgaag ccgcgcgtca gccgccagtt
     1861 tgaaatcggc gtgcgtaaaa actggggcga ttttgtaacc acgttgaacg cgttccaaat
     1921 caaacgcccg ggctactggc gcggcaatac caaaaaaggt actgattttg cagcatataa
     1981 agcagcagga ggcgcagccg gcgacgagca aggtatggaa cgcaatcgcg gtattgagtt
     2041 caatgcctat gccaacctgt tgaacaaaac cctgcgcccg actttcggcg tgatgtatct
     2101 gcaatcgtcc gtgaagaatt atccgaatgc gcgcgatatg ctggttaacg gcgtacaagt
     2161 cgccaacccg cgcgtgattg tcaaaacagg cgttgaacgg gatacaccgt ttgccaaagg
     2221 tttgagcttg aacggcaatg tttcgtattt cggcaagtct tatcaagaca cgcaaaaaca
     2281 atacgccttc ccgtcctata ccttggttga cgtaggcgcg cgctacaaaa ccaagctggg
     2341 caaaaacacc ctgaccgtca gcagctcggt agaaaacctg ttcaacaaaa actactggca
     2401 ggtacagcgc ggacaatttg accgtagttt cgccgtcgtc ggtctgccgc gtacttactg
     2461 gctgaaggcg gaattggatt tctaagctgc ctccgtttcc gaaaatgccg tccgaacagg
     2521 tttcagacgg catttctttt aaagaaatat ggcgggatat gcttaaataa tgctattcta
     2581 gtacctttga aacctgcttg tttgaagtga tgaaaaaacc tttgatttca gttgcggcag
     2641 tattgctcgg cgttgctttg ggtacacctt attatttggg tgtcaaagca gaagaaagtc
     2701 tgacgcagca gcaaaaaata ttgcagaaaa cgggcttttt gaccgtcgaa tcgcaccagt
     2761 atgatcgagg ctggtttacc tctacggaaa cgacggtcat ccgtctgaaa cccgagttgc
     2821 tgcataatgc gcagaaatac ctgccggata acttgaaaat agtgttggaa cagccggtta
     2881 cgctggtaaa ccatatcacg cacggccctt tcgccggcgg attcggcacg caggcgcaca
     2941 ttgaaaccga gttcaaatac gcgcctgaaa cggaaaaagt tttggaacgc ttttttggga
     3001 aacaagttcc ggtttccctt gccaataccg tttatttcaa cggcagcggt aaaatggaag
     3061 tcagtgttcc cgctttcgat tatgaagaac tgtcgggcat caggctgcac tgggaaggcc
     3121 tgacggggga aacggtttat caaaaaggtt tcaaaagcta ccgcaacagc tatgatgcgc
     3181 ccttgttcaa aatcaagctg gcagacaaag gcgatgccgc gtttgaaaaa gcgcatttcg
     3241 attcggaaac ttcagacggc atcaatccgc ttgctttggg cagcagcaat ctgactttgg
     3301 aaaaattttc gctcgaatgg aaagagggtg tcgattacaa cgtcaaattg aacgaactgg
     3361 tcaacctcgt taccgatttg cagatcggcg cgtttatcaa tcccaacggc agcatcgcac
     3421 cttccaaaat cgaagtcggc aagctggctt tttcaaccaa gaccggggaa tcgggcgcgt
     3481 ttatcgacag cgaagggcgg ttccgtttcg atacgttggt gtacggcgat gaaaaatacg
     3541 gcccgctgga catccatatc gctgccgaac acctcgatgc ttctgcctta accgtattga
     3601 aacgcaagtt tgcacaaatt tctgccaaaa aaatgactga ggaacaaatc cgcaatgatt
     3661 tgattgcggc agtcaaaggc gatgcttccg gattatttac ccatgacccg gtactaaata
     3721 tcaaaatttt ccgtttcacc ctgcctcagg gaaaaattga tgtgggcgga aaaatcatgt
     3781 ttaaaggcat gaagaaggaa gatttgaacc aattgggact gatgttaaag aaaaccgagg
     3841 caaacatcag aatgagtatt cctcaaaaaa tgttggaaga tttggcggta agtcaggctg
     3901 gaaatatttt cagtgtaaat gccgaagatg aggcggaagc cagagcaagc attgccgata
     3961 ttaatgaaac attgcgcctg atggtggaca gtacggtcca aagtatggca agggaaaaat
     4021 atcttacttt agacggtaat cagattgata cggtcatttc ccttaaaaac aacgccctga
     4081 agttaaacgg gaaaacgctg caaaatgaac ccgatcctga ttttgacgag ggagatatgg
     4141 tttccggcca gccgcattaa tgccgtttat gccaaatgcc gtctgaaatg ttcagacggc
     4201 atttttgtcg gtctcgaaat aggatggatt ttcatgtttc aaaaacaatt gattgtctct
     4261 aatccgatat caggacacgc ttccaatgtt tagatatgga attcggacgg gataggggaa
     4321 ctattgaaat ccagttgaaa tctgttattg gaaagcatgg aggaaaaata aaaggctgta
     4381 ttttaatata gtattgatag gcatgcttag cttcaaggta ttcaatagtg taggtgtctg
     4441 cctaactgga aaataatcgt cttttccctt gcaaccagtt aaaattgaca gatggttaaa
     4501 acacagccaa ataaaaatgc cgcctgaagg gttcagacgg catttgttcg gataaacctt
     4561 atcggctgct tagagcatca ggtttatttt ttcttttgag gaggcaggtc ggtacataca
     4621 cccaaagcca cttccgcagc cataccgatg gattcgccca aggtcgggtg cgggtggatg
     4681 gttttgccga tgtctgccgc gtcgcagccc atttcgatgg caaggcagac ttcgccgatc
     4741 atgtcgccac cgttcggacc gacaatgccg ccgccgatga tgcggccggt ttcggcatcg
     4801 aaaatcagct tggtaaagcc gttgtcgcaa ccgttggcaa tcgcacgacc ggaagccgcc
     4861 catgggaagt tggctttggt gattttgcgg cctgatgctt tggcagacaa ttcggtttca
     4921 ccgacccatg ccacttcggg ggaagtgtag gcaacgccgg gaataacgcg tgcgtcgaag
     4981 taggctttgt ggccggcaca gttttcagcg gcaacgtgac cttcgtgaac ggctttgtgt
     5041 gccaacatcg gttgaccgac gatatcgccg atagcgtaga tgtgcggtac gttggtgcgc
     5101 atttgtttgt caacttcgat gaagccacga tcggtaacgg caacaccggc tttttctgcg
     5161 ctgatgagtt tgccgttagg cgcacggcct gccgcgacca gtacggcatc gtaacgttgc
     5221 ggctctttag gtgcatttgc accttcaaag gtaacgtaaa cgccgtcttc tttcggctcg
     5281 actgcaacgg ttttggtgtt gaccataatg ttgtcaaaac ggtattcgtt ttgtttttgc
     5341 catactttaa ccaagtcgcg gtctgcgcct tgcatcaggc cgtccatcat ttcaaccaca
     5401 tccaggcgag aacccagcgt gctgtaaacc gtacccatct cgaggccgat aatgccgccg
     5461 ccgataatca gcagtttgcc cggtacttct ttcagtgcca gtgcgccgct ggaatcgatg
     5521 atgcgcggat cttcaggaat gaaaggcagt ttggttacgc ggctgcctgc tgcaatgata
     5581 cagtttttga aggcaacgat ttttttctcg ccggtagggg ctgcctgttc gtacgcgtcg
     5641 ccggcagtca gcgacacttc caagtggtga ggatccaaga attgcccgtc gccttggata
     5701 acgtccactt tacggctttt cgccatacct gccaaaccac ccgtgaggcg ggaaaccacg
     5761 ccgtctttat aggcgcgaag catatcgatg tcgagttccg gttcggggta tttgataccg
     5821 ttggcagcca agtggcgcac ttcgtcgata acggcggcat tgtgcaacaa ggctttggaa
     5881 gggatacagc cgacgttcag gcaaacgccg cccaaagttt tgtaacgctc gacgatggcg
     5941 actttcaagc cttcatcggc agcggcaaat gcagcggagt aaccgccggg accgccgccc
     6001 aatacgacca cgtcgtactc ggcatcggca gcactgccga attgcgcggc ttgcggagca
     6061 ggagcggcag ctttgggtgc ttcttgcgcc ggggcggcag ccgcttcggc tttaggggcg
     6121 gcagccgcac cttcggcttc gacgacgaca atcaaaccgc cttcggagat tttgtcgccg
     6181 actttgactt tcacttcttt gatcacgccc gcaacttcgg caggcacgtc catcgtcgct
     6241 ttgtcggttt ccaaggtaat cagggtgtcg tcaacggcga tggtgtcgcc aacgttcact
     6301 tcaaccgcga taatatctac attttcgtgt ccgccaatgt cgggcacttt caattcaact
     6361 aaagccatta tattgacctt tcaacttagt taatttctat caactcttta gcaataatca
     6421 cttcttcagg aatacagcca caatcttcag ctctgattgt tacttccttc tcatctgggt
     6481 tttctggatg ttcaacctca tattcaaaaa tagaatattg accaaacaaa tctctattta
     6541 aaacatatat atagccattt tcgatgccgg agcttgttgc aaatttcttg gcaatttcct
     6601 tgtctgttgt cgtagatata taacatccgt catatagatc tgtttcaatt tgatgggcgt
     6661 aaactgcatt cttcacactt ggaccatgtg tagctttacc atcatattta aacttaccat
     6721 cataacgaat tgcaacttca gctttattac ctttaggttt taactgtcca ttattttgct
     6781 catcttgttg gcaactaatg cctctatata aataagaaat ttcccataag ttattaattt
     6841 aaagtttgaa tttaacacca tcactcatca atctcaagca atccaaacat tatcagactg
     6901 cttgagaggg atgttttatt ttataaggta atgcggcgga agtctttcaa caggttcgcc
     6961 aggaatacgg tgaagcgcat accggcggca ccgtcgatga cgcggtggtc gaaggacagg
     7021 ctcaacgggc acatcaggcg cggggcaaac tctttgccgt tccaaaccgg tttgatttgg
     7081 gatttgcaca cgcccaagat agcgacttcg ggagcgttca caatcggcgt gaaacctgtg
     7141 ccgccgatgc cgcccaagct ggaaatggta aagcacgcgc cttgcatttc ttgcggtttg
     7201 agcttgcctt cgcgggcttt tttggacaat tcggtcaatt cttggctgat ttgtttcaag
     7261 cctttttgat ccacgtcttt gatgacggga acaaccaagc cgttcggcgt atcggctgcg
     7321 aaaccgatgt tgaagtagtt tttcagcacc aggttgtcgc cgtccaaaga agcgttgaat
     7381 tcggggaagg ctttcagcgc ggaaacggag gctttgatga tgaacgccaa cggggacagt
     7441 ttcacgcctt cgcgttccca ttctttgttc agctgtttgc ggaattcttc cagctcggtc
     7501 atatccgctt cttcgtgtac ggtaacgtgc ggaatcacca cccagttgcg ggacaggttt
     7561 tgaccggaga ttttcttaat gcgggacaat tctttaactt cgacattgcc gaatttggag
     7621 aagtccactt taggccacgg cagcaagtcc agaccgccgc ccaaagatgc gccggctgcg
     7681 gcaggttttg ccgcgccgcc ctgcattacg gatttcacaa aggctttgat gtcgtctccc
     7741 atgatacggc ctttcaagcc gctacctttg acttggccca aatccacgcc caattcgcgc
     7801 gccagtttgc gtgcggaagg accggcgtgt gctttggcga aagcggcttc atcgattttg
     7861 gcagcggcag gcgaggcaga tgtaggcacg gcggcgggtg cggcagcttg agcggcaggc
     7921 gcaggagctg ctgcggcaga gccggcggtt tccacttcga taatggcaga gccttcggac
     7981 actttgtcgc cgacttttaa gaatacggct ttaacgacac cggcagcggt acaaggtacg
     8041 tccattgtcg ctttatcggt ttccaaagta atcagcgtgt cgtcttcggc aacggtgtcg
     8101 ccaactttga tttcaacggc gattacatct acatcggtat ggccgccgat atcgggaacg
     8161 gctacttgaa cggttgcacc gcctgcggca gcgggtgcag gttgtgcttc ggcggcagca
     8221 gccggggcgg cttcggcagc ggcggcaccg gtttcaacgg tcaaaattac gccgccttcg
     8281 gagattttgt cgccgacttt gacttttact tctttcacga caccggccgc atcggcaggc
     8341 acatccatcg tggctttgtc ggtttccagt gtaatcaggg tgtcgtcaac ggcgatggtg
     8401 tcgcccgctt taacttctac ggcgatgatg tcgacgcttt cgtgaccgcc gatatcgggg
     8461 actttgattt ctacgatact catttgagtt cctttaatct ttttcagttg gatgccgtct
     8521 gaaagctgct tcaggcggca tcggtacggt ttgtctgttc aaaccattcg ggcattcggt
     8581 tttcagacgg cctgatgttg gcatgaaaca aacaggccgt ctgaaacatc aatcagcgtt
     8641 tccagctagg ggctgaatcg gctttgatgc cgtatttctc aatggcttgt tgaacggttt
     8701 ctttgctgac tttgccttgt tccgccaatg cggccaatgc tgccacggca acgttgtagc
     8761 ggtccacttc aaagaagcgg cgcaggttgg cgcggctgtc ggaacggccg aaaccgtcag
     8821 tgcccaagac atggtagtcg ttcgggatgt acgcgcggat acggtcggca tagctgcgga
     8881 tatagtcggt agcggcgata accggaccgt catgaccttg cagttgggaa gtaacgaaag
     8941 gcactttttc agcttccagc ggatgcaggc ggttgaagcg ttcggtttcg atggcgtcgc
     9001 ggtgcagcag gttgaaggac gggcaagacc agatgtctgc ttccacgccg aagtcggctt
     9061 tcagcagctc ggcaccggca atcacttctt gcagaatcgt accggaaccc atcaattgga
     9121 ctttcttgtc gcctttgccg ccggctttca gcaggtacat acctttgagg atgtcttgtt
     9181 ccgcaccttc cggcatatcg ggatgggtgt agttctcgtt catcagggtg atgtagtaga
     9241 acacgtcttc attattggca tacatacggc gcagaccgtc ttgtacgatg acggccactt
     9301 cgtattggaa ggtcgggtca taagatacgc agttcggaat caggtcggcc tggatgtggc
     9361 tgtggccgtc ttcgtgttgc agaccttcgc cgttcagcgt cgtacggccg gcagtaccgc
     9421 ccagcaggaa gccgcgcgcg tgcatatcgc ccgccgccca agccaagtcg ccgatacgtt
     9481 ggaaaccgaa catggagtag tagatgtaga acggaatcat cgcgaagtcg ctgttggcgt
     9541 agctggtcgc agccgcaatc cagtcggcca tcgcgcccgg ttcgttaata ccttcttgca
     9601 agatttgacc gtcgacggat tctttgtaga acatcagttg gtctttatct tgaggcgtgt
     9661 attgttggcc tttgggattc caaataccgt attggcggaa cataccttcc atgccgaaag
     9721 tacggctttc gtcgggaacg ataggtacga cgcgtttgcc gatttttttg tctttcagca
     9781 aagtggacag gatgcggacg aatgccatcg tggtcgagaa ctcgcgttca ccgctggatt
     9841 tgagttgtgc gtcgaatgcc gacagctcgg gcacttccaa tacttcctgc gtcggtttgc
     9901 gttgcggcag gtagccgccc aaagcatcgc ggcgtgcgtg caggtatttg tattcttccg
     9961 tatcgggggc aaaagtcagg taaggcagat cgccgctttc gatttgttcg tcggtaaccg
    10021 gaatgtcaaa gcggtcgcgg aattgtttca gggacgcttt gtccattttt ttggcttggt
    10081 gggcaacgtt ctgaccttcg ccggatgcgc ccataccgta acctttaatg gttttcgcca
    10141 agataacggt aggtttgccg tcggcatggt tcgctgcgcg gtcgtaggcg ttgtacactt
    10201 tttgaggatc gtggccgccg cggttcaatg cccagagttg ctcatcggtc atatcggcaa
    10261 ccaatgcttt cagttcgggc gtattgaaga agtgttcgcg cacatacgcg ccgtctttgg
    10321 atttgtaagt ttggtagtcg ccgtccaaac attcttccat acgttggcgc aggataccgt
    10381 ctttgtcttt cgccaagagg cggtcccaac ggcggcccca aatgactttg acgacattcc
    10441 agccggcacc ggcaaagttg ccttccaatt cttggatgat tttgccgttg ccgcgcaccg
    10501 gaccgtccaa gcgttgcagg ttgcagttga tgacgaaaat caggttgtcc aagccttcgc
    10561 gtgcagccag cgcgattgca ccttgggatt cgggttcgtc catttcgccg tcgccgcaga
    10621 aacaccatac tttacggcct ttggtttttg ccaagccgcg tgattccaag tatttcagga
    10681 aacgcgcctg ataaatcgcc atgatggggc ccaagcccat ggatacggtc gggaactgcc
    10741 agaagtcggg caagaggtgg gggtgtggat aggaaggcag accgtgtccg tccacttctt
    10801 ggcggaagtt gttcagctgg tcttcggtca gacggccttc gacgaatgcg cgtgcataga
    10861 tgcccggggc aacgtgacct tggaagaaga ccaaatcgcc ttcttcacct tcacctttgg
    10921 ctttccagaa gtggttgaaa ccgacttcgt acatggtggc ggcagattgg aaagatgcga
    10981 tgtgcccacc cagttccaaa tctttcttgc cggcgcgcaa tacgatggcg gcggcgttcc
    11041 agcgtacgaa tgcgcgaatg cggtgttcga tgttttggtc gcccggaatg cctttttcgt
    11101 tttcaaccga aacggtattc aaatacgggg tggtcgtgcc gtggggcata cgtacgcctt
    11161 tgtcgcggca gtatttgagc aggttttcca agaggtattg cgcgcgttcg ccgccttcat
    11221 attcgaggac ggagcttaac gcgtccagcc actcttgggt ttcgataggg tcaacatcgt
    11281 gtaattgggt ggacatagta tctatccttt atgttgagtg tttaatcgga agggagggtg
    11341 tagccaaccc ctttattttc gtttgtgaaa aataataagc aaaaacgaaa atttctaaat
    11401 caaaccgaaa caaaataatt taagtttaat gttttcaaaa aattataaaa gtcttggaag
    11461 caagggaaaa cttgaaaaaa aaaatgttcg gtctgctccg cacgaaatcc tatcagcaat
    11521 cgggtctgct ggcaaataac catgctttta aaagcaggtt ttatactgaa gttggcgttt
    11581 ggtaaggaca ggtctttttg attgcgaaaa cattctgttt aattttggtt atattttttc
    11641 ggaattgtgg gatatcgaat cgttatgtct ctgtcggcaa ttttcctatt gcagaaaatc
    11701 tatacacaag ctttgtggac aaccgtgttg ataagccacg gcttgtgtta aaaaagtctt
    11761 acgcaacaaa tgtacgctta ttttgattaa aaattaggca aatttaaatc tataaaaatc
    11821 atgtaattat gttcaatatg atgtttctgg tcatgttttt gccgtctgtt atggggtttg
    11881 tcccgcgttt ggggcagggg gtgaattttg gaggttggtg caaaacctgc ttttttcgcg
    11941 ttacaatctg cacccgattt acttttgaat ctgcctgttt ggaaaaatac ttttatgaaa
    12001 gccagccaat tctttatctc tactttaaaa gaagcccctg ccgaagccgc gtttgccagc
    12061 cacaagctga tgattcgcgc cggtctgatt aaagccaacg cgtccggtct ttatacttgg
    12121 atgccgatgg ggctgcgcgt gttacgcaaa gtcgaaaacg tcgtgcgcga ggaaatggcg
    12181 cgcgcgggca gcgtggagct gctgatgccg gtggtgcagc ctgccgaact gtggcaggaa
    12241 tccggccgct gggagtttta cggtaaagaa ctgctgcgcc tgaaagaccg ccacgaacgc
    12301 gatttctgca tgggcccgac ctgcgaggaa gtcatcgccg acatcgtgcg caaagaaatc
    12361 aacagctaca aacaactgcc gaaaaatttt taccacatcc aaaccaaatt ccgcgacgaa
    12421 gtgcgcccgc gtttcggcgt gatgcgcgcg cgcgaatttg tgatgaaaga tgcttattcc
    12481 ttccacgccg actacgcctc gcttcaggcg acctatgatg ccatgtatga cgcttactgc
    12541 cgcatcttta cccgtctggg tttggcgttc cgccccgtcg ccgcagacac cggcagcatc
    12601 ggcggtacgg gttcgcacga gtttcaagtg ttggcggaaa gcggcgaaga tgtgattgca
    12661 tacagcgaca cttccgatta cgccgccaat atcgagttgg caccgacctt gccgcttaaa
    12721 ggtgaacgtg ccgccgctca ggctgtgttg accaaagtac atacaccaaa cgtcaaaacc
    12781 attgagtctt tggttgaatt cctgaatatt ccggttgaac aaaccctcaa atccatcgtg
    12841 gttgaaggcg aaaacgaagg cgaactcgtc ctactgctgt tgcgtggcga ccatgagttt
    12901 aacgacatca aggcagaaaa actggcgggc gtaaaatcgc cactgactat ggcaagccct
    12961 gccgcgattg ttgaacaatt cggcgcaaac ggcggttcgc tcggcctcgt cggcttcaca
    13021 ggcaaagtct atgccgattt cgctaccgaa aaaggcgcgg actgggttat cggcgcaaac
    13081 gaagacgact accactatac cggcttcaac ttcggccgcg atgccgccga gcctgaattc
    13141 gtcgatttgc gtaatgtggt cgaaggcgac gaaagccccg acgggcaagg ccgtctgaaa
    13201 ctggcgcgcg gcatcgaagt cggacatgtc ttccagttgc gcggcaaata tacccaagct
    13261 atgaacgtca gcttcctcga caataacggc aaatcgcaaa tcatggaaat gggctgctac
    13321 ggcatcggca tcacccgcgt cgttgccgcc gccatcgagc agaataacga cgaaaaaggc
    13381 atcatttgga ccaaagcgat ggcgccgttt gaagtcgtca tcgtgccgat gaactacaaa
    13441 aaatccgaca ctgtgcgtga agccgccgac agaatctatg ccgaattgct ggcggcaggc
    13501 gcggatgtgc tgctggacga ccgcgacgaa cgcgcaggcg tattgctgaa cgattccgag
    13561 cttctcggta tcccgcaccg catcgtcatc ggcgaccgcg ccttgaaaga aggcaatgtc
    13621 gaatacgccg aacgccgcga caacgaagcg caggcagttg caatcggaga aattgttgcg
    13681 cgtgtaacag cttcattaaa tgcgtaagtc tggtcaaatg ccgtctgaaa ataatttcag
    13741 acggcatttt tattttgggg gaaagtgaag tggcttcagt atttttagaa ggagaagcag
    13801 tcgaggtcgg caatattttc tgtatcggca ggaactatgc cgcacatgtc gaagagttaa
    13861 aaaacgaaat accttcagaa cctgtggtgt ttatgaagcc gtcaggcagc attttgaaca
    13921 gcggcggtac aatcttgctg cccgagttca gtagggatgt gcagtttgag tgcgaactag
    13981 tgttgctgat tggccgggat tcagacggca cgggcgaggg tgaagatatt ttgggatgcg
    14041 tggcggggta cggcgtgggt ttggatttga ccgcgcggga catccaatgc cgtctgaagg
    14101 cgcaggggct gccctggctg aaggcaaaag ggttcaggca ttctgcgtgc gtgtcggatt
    14161 ttgcggcggc agacagaatc ggcaatcaag ataaggtgtt gttttcatta aaacaaaacg
    14221 gcgtattgaa acagcgcggg gagactggtt tgatgattta cccgattcgg gaaattttac
    14281 acaaactggc ggcggattac ggtttgggga aaggggattt ggtgtttacc ggaacgccct
    14341 ccggcgtggg cgcaatcggt gcgggggaca atttggcatt ggagctggac ggcttggtgt
    14401 gcgcctcttt cacgataggg tgttaaccgc gccgcttaac cttcacgccc gttgaaacat
    14461 tccgccgggc cgccctcgag aaaaccgtgc aggatggctt gcgccgccac ttggtcgagc
    14521 accgatttgc gttttttgcc caagacctgt gcttccgaaa gcaggctttc ggcatagacg
    14581 gacgacagcc gttcgtcaac ccaatagacg gggagattga acctgccgtt cagcctgcgt
    14641 ccaaacttgc gcgacaggtg cgtcatttca tgttccgtgc cgtcggcatg cacgggcagt
    14701 ccgacgacaa aataacgcgg ctgccattct tgaaccagct tggcgattgc cgcgaatttt
    14761 tcatcgttgc tgccgccggt aacggtggcc agcggatggg acagccccaa ttccgcatcg
    14821 ccctgcgcca cgccgatgcg cgcttcgccg aagtcgaatg ccagtgccgt tccttttgga
    14881 attttatgca tggccggctc ccgaaaacag ggcgagcggg tcgatgccga gtttggcgaa
    14941 tgcggcggcg taacggtgtt cgtaggggat gtcgaacagg atgtgttcgt cggcgggaac
    15001 ggtcagccac gcattgtcgg caagttcgcg ttcgagctgc cctttgctcc agctcgaata
    15061 gcctatgctg atcaaggctt tgtcaaccgc gccttcgcgt gaaatgtttt caagcacatc
    15121 gcgggaagag gtcagcgcga tgccgtctga aacgccgatg ctgctttgcc agttgccgat
    15181 cggagtgtgg acgacgtaac cgcgctcgac ctgcaccggc ccgcccatca tcacgctgtc
    15241 gtgctgcatc cgcatgggga tgtttttgcc ggtggcggaa aaaatcatgt ccatcgtaat
    15301 cggagagggt ttgttgatgg cgataccgag tgcgccgtct tcatcgtgtt tgcagatata
    15361 gacgaccgat tgtgaaaaaa acgcgtcttc catatcgggc atggcgacga gaaagtggtt
    15421 ggacaggttc atatcagcgt ttgtcgggat tttttatcgg gttggaaata atgcaggcgc
    15481 cggcaagcag tttgccggaa gtatcggctt gcaccccgtc ggtttgcggt atgccgctgc
    15541 cgaaacagga aaccgcgctt aaggaatttt tcggcgatcc ttggacgatt ttatcgctgt
    15601 aaaccaaata ggcgaaggct ttgcgtttgg ggtcgtaata acggacaatc tgccggctct
    15661 tgaacgcgaa gcccgtaccg cgcttgaaaa cttctttcgg tttgcgcacg gcggtttcgt
    15721 caaaagaaat cgaagatgcc gtctgaacgc acgaaaccga tgcgtcggac gcgtcctctt
    15781 ccaaattgac catttccttc aagccgcctt tttttgcata cgaaatataa caggcaaccc
    15841 cttgaacgtc gggatcgtcg aatccttcca cttcgatacg gtcgtttttg cccaacatgt
    15901 tgaaaacggt acttgcccgt ccgattttat cggtttcgcc gctgccgcag gcagtcggca
    15961 ggacggcggc agacagcagt agcagtctgt tcatcaaaat ttccttatcg gttgaatccc
    16021 cgttttccgg aaggcggcag ggtttgcttt gtttggaggg gcgcacgctc ccaaatcggg
    16081 caacatacgg tacgcagctt acatgtgcta ccctgtgtgt cggaacacaa tatccgttgc
    16141 agcattgaaa atatgccgca caatataagg ctgtttgagc cgattgcaag tatccgcgca
    16201 aagaaggtgc aagattgggc aaacggcatt ttcagacggc ataactgaca gtataatccg
    16261 aacatccggc cgctttcgcg cggccttcag cattatccga ttttttctga aagccgaacc
    16321 atgcaataca aacccctcct gcttgccctg atgcttgtct tttccgcccc cgccgttgcc
    16381 gcccacgacg cggcacacaa ccgttccgcc gaagtgaaaa aacaggcgaa gaacaaaaaa
    16441 gaacagcccg aagcggcgga aggcaaaaaa gaaaaaggca aaaatgccgc agtgaaagat
    16501 aaaaaaacag gcggcaaaga ggcggcaaaa gagttcaaaa aaaccgccaa aaaccgcaaa
    16561 gaagcagaga aggaggcgac atccaggcag tctgcgcgca aaggacgcga aggggataag
    16621 gaatcgaagg cggaacacaa aaaggcacat ggcaagcccg tgtccggatc caaagaaaaa
    16681 aacgcaaaaa cacagcctga aaacaaacaa ggcaaaaaag gggcaaaagg acagggcaat
    16741 ccgcgcaagg gcggcaaggc ggaaaaagac actgtttctg caaataaaaa agcccgttcc
    16801 gacaagaacg gcaaagcagt gaaacaggac aaaaaataca gggaagagaa aaatgccaaa
    16861 accgattccg acgaattgaa agccgccgtt gccgctgcca ctaatgatgt cgaaaacaaa
    16921 aaagccctgc tcaaacaaag cgaaggaatg ctgcttcatg tcagcaattc cctcaaacag
    16981 cttcaggaag agcgtatccg ccaagaacgt atccgccaag agcgtatccg tcaggcgcgc
    17041 ggcaaccttg cttccgtcaa ccgcaaacag cgcgaggctt gggacaagtt ccaaaaactc
    17101 aataccgagc tgaaccgttt gaaaacggaa atcgccgcta cgaaagcgca gatttcccgt
    17161 ttcgtatcgg ggaactataa aaacagccag ccgaatgcgg ttgccctgtt cctgaaaaac
    17221 gccgaaccgg gtcagaaaaa ccgctttttg cgttatacgc gttacgtaaa cgcctccaat
    17281 cgggaagttg tcaaggattt ggaaaaacag cagaaggctt tggcggtaca agagcagaaa
    17341 atcaacaatg agcttgcccg tttgaagaaa attcaggcaa acgtgcaatc cctgctgaaa
    17401 aaacagggtg taaccgatgc ggcggaacag acggaaagcc gcagacagaa tgccaaaatc
    17461 tccaaagatg cccgaaaact gctggaacag aaagggaacg agcagcagct gaacaagctc
    17521 ttgagcaatt tggagaagaa aaaggccgaa caccgcattc aggatgcgga agcaaaaaga
    17581 aaattggctg aagccagact ggcggcagcc gaaaaagcca gaaaagaagc ggcgcagcag
    17641 aaggctgaag cgcgacgtgc ggaaatgtcc aacctgaccg ccgaagacag gaacatccaa
    17701 gcgccttcgg ttatgggtat cggcagtgcc gacggtttca gccgcatgca gggacgtttg
    17761 aaaaaaccgg ttgacggtgt gccgaccggg cttttcgggc agaaccggag cggcggcgat
    17821 gtttggaaag gcgtgttcta ttccactgcg cctgcaacgg ttgaaagcat tgcgccggga
    17881 acggtaagct atgcggacga gttggacggc tacggcaaag tggtcgtgat cgatcacggc
    17941 gagaactaca tcagcatcta tgccggtttg agcgaaattt ccgccggcaa gggttatacg
    18001 gtcgcggcag gaagcaaaat cggcacgagc gggtcgctgc cggacgggga agaggggctt
    18061 tacctgcaaa tacgttatca aggtcaggtg ttgaaccctt cgggctggat acgttgatcg
    18121 gattgccgaa acggcaggct ttatgcaaat cgtgccgata tggtgcgctg ttccgtgaag
    18181 tttgccgttt gccgtacctg tgttttccgg tttttggaaa tcaagagaga aagttaaaga
    18241 agaatgtcga aacctgtttt taagaaaatc gcactttata ctttgggtgc aatcagcggc
    18301 gtggccgtaa gtctggcggt gcagggtttt gccgccgaga aggacgggcg ggataacgaa
    18361 gtcctgccgg tgcaatccat ccgtacgatg gcggaggttt acggtcagat taaggcaaac
    18421 tactatcatg acaaacccga tgccgatttg tttgaaggtg cgatgaaggg tatggtggcc
    18481 ggtttggatc cgcattccga atatatggat aaaaaaggtt atgccgagat aaaggagtcc
    18541 accagcggcg aatttggcgg cttggggatg gaaatcgggc aggaagacgg ttttgtcaaa
    18601 gtggtttcgc cgattgagga cacgcctgcc gaacgggcgg aggtgaaaag cggcgatttc
    18661 attgtgaaaa tcgataatgt ttcgacgcgc ggtatgacgg tcagcgaagc ggtgaaaaaa
    18721 atgcggggca agccgggtac gaagattact ttgacgctgt cgcgcaaaaa tgccgacaag
    18781 ccgatagtcg tcaacctgac ccgtgccatt attaaagtga aaagcgtccg ccatcacctg
    18841 atcgaacccg attacggcta tatccgcgtg tcgcagttcc aagagcggac ggtcgaaagc
    18901 gtcaataccg ccgcaaaaga gctggtaaag gaaaataaag gaaaaccgct caaggggctg
    18961 gtgttggatt tgcgcgacga ccccggcggg cttttgaccg gcgcggtcgg cgtgtcggcg
    19021 gcgtttctgc cgtctgaagc ggtcgtcgtc agcaccaagg gacgcgacgg caaagacggc
    19081 atggtactga aagccgttcc cgaggattat gtgtacggta tgggcggcga tcctttggcg
    19141 ggtattcctg ccgagttgaa aacgattccg atggcggtat tggtcaattc cggttcggct
    19201 tccgcgtcgg agattgtcgc cggcgcattg caggaccaca aacgcgcggt catcgtcggt
    19261 acgcagagct tcggtaaagg ttcggttcag actttgattc ctttgtccaa cggcagcgcg
    19321 gtcaagttga cgaccgccct gtattacacg ccgaacgacc gttccattca ggcacagggg
    19381 attgttcccg atgtcgaagt aaaagataag gaacgtactt ttgaaagccg cgaggcggac
    19441 ctggtcggac acatcggcaa tcccttgggc ggcgaggatg tgaacagtga aacccttgcc
    19501 gtaccgcttg aaaaagatgc ggataagccc gctgcaaaag aaaaaggtaa aaagaaaaag
    19561 gacgaggatt tgtcttcaag gcggattccg aaccctgcga aagacgatca gttgcgtaag
    19621 gctttggatt tggtcaagtc gcccgagcag tggcagaagt ctttggggct ggcggcgaaa
    19681 aagccggttt caaataaaga taaaaaagat aagaagtagg ttttgacgat gccgtctgaa
    19741 agggtttcag acggcatatt atgtttgaaa ggacaggtta tgagcgattt tttaccggaa
    19801 tttgaaccgt gcggcattta tagtggatta acaaaaatca ggacaaggcg acgaagccgc
    19861 agacagtaca aatagtacgg caaggcgagg taacgccgga ccggtttaaa tttaatctac
    19921 tatacttggg tagcgattgg gtttcagacg gcatacattc aggatttcac cgccttatac
    19981 cgtctgaaag acgtttctga cccatcccta ctacacgctt tgtccgcaaa cgtatcacag
    20041 gcaagattat ggaagtcatc cgatacccaa attccccctt caaactccac caacccttcc
    20101 cgcccgcagg cgaccagccc accgccattg cgggcctgct cgaagggctt tcagacggcc
    20161 ttgcctacca aaccctgctc ggcgtaaccg gttcgggcaa aacctacacg atggcgaacg
    20221 tcatcgcgca aagcggcaga cccgccatca ttatggcgca caacaaaacc cttgccgccc
    20281 agctttatgc cgaaatgcgc gagtttttcc ccgaaaacgc agtggaatat ttcgtgtcgt
    20341 attacgacta ttaccagccc gaagcctatg tgcccagccg cgatttgttc atcgaaaaag
    20401 acagcgcgat caacgaacac atcgagcaga tgcgcctttc cgccaccaaa aacctgatga
    20461 cgcgcgacga cgtgattatc gtcgccaccg tgtccgccat ttacggtatc ggcgacccga
    20521 ccgagtatca acaaatggtg ctgtccgtaa aagaaggcga caccatcgag cagcgcgaca
    20581 tcatcgccac gctcgtttcc atgcagtacg aacgcggcga tttggacttc aaacgcggca
    20641 gcttccgcgt gcgcggcgac gtgattgacg tgtaccccgc cgaaagctcc gaaaacgcct
    20701 tgcgtatcag cctgtttgat gacgaaatcg accgcctcga tatgttcgac ccgctttcag
    20761 gcagcctgca ccagcgtgtc ggacgctaca ccgtcttccc gtccagccac tacgttaccc
    20821 cgcgcgacac cgtattgcgc gcctgcgaat ccatcaaaga agaattgcgc gaacgcatcg
    20881 aatttttcgc tcgcgaacaa cgccccgtcg agcaacaacg catcgaacag cgcacccgtt
    20941 tcgacctcga aatgctctac gaaatgggct tctgcaaagg cattgaaaac tactcccgcc
    21001 acttctccgg taaaaaagaa ggcgaaccgc cgcccacgct gatggactac ctgcccgaca
    21061 acgccatcat gttcatcgac gaaagccacg ttaccgttac ccaaatcggc ggcatgtaca
    21121 aaggcgacgc attgcgcaag caaaacctcg tggactacgg cttccgcctg ccttccgccc
    21181 gcgacaaccg cccgctcaaa ttccacgaat ttgaaaaagt catgccgcaa accgtcttcg
    21241 tttccgccac ccccgcgaaa tacgaagaag aacacgccgg acaagtcgtc gaacaagtcg
    21301 tccgccccac agggctggtc gacccccaaa tcatcatccg ccccgtcgcc acccaagtcg
    21361 acgatttaat gagcgaaatc aacgaccgta ttcaaaaagg cgaacgtgtg ctcgttacca
    21421 cccttaccaa acgcatggcg gagcaactca ccgactatta cagcgaactc ggcatcaaag
    21481 tgcgctacct gcacagcgac atcgacaccg tcgaacgcgt tgaaatcatt agagatttgc
    21541 ggctcggcct gtttgacgta ctcgttggca tcaacctgtt gcgcgaaggc ctcgacatcc
    21601 ccgaagtctc cctcgtcgcc atcctcgatg ccgacaaaga aggcttcctg cgctcccacc
    21661 gcagcctgat tcaaaccata ggccgcgccg cgcgcaacgt gaacggcgtc gccatcctgt
    21721 acgccgacaa aattaccgac tccatgaaag ccgccgtcga cgaaaccgaa cgccgccgcg
    21781 aaaaacagat taaattcaac gaagaacacg gtatcgtgcc gcagcagatt aaaaaacagg
    21841 tcaaagacat catcgacggc gtgtaccacg aagaagacag tggcaaaggc cgtagacaag
    21901 gcaaaaacaa ggtcaaagtc ggcgaaatcc acaacgaaga agacgcgatt aaagaaatcg
    21961 ccaaactgga aaaagccatg cagcaggcgg ctagggattt acagtttgaa gaagctgctg
    22021 tgttgaggga taggattagg aatattaaag agaatttgtt gtttggggcg gagtgatttt
    22081 gaaatagtaa tcgcagcctg aatacatagc gtgtgcgcca ctggcgcgcg ctcattgtgt
    22141 tatcaggaat ccttgaaatc atgtacgcag cttgcatcac acatcctagc tatacataaa
    22201 caacaaggcc gtctgaattt tcagacggcc ttttccgttt acaactttac tacttattca
    22261 ataactacac gtgcattcag aggctgtacg gggcggttgt tttccgaacc gacggcgcgg
    22321 gtgaattttt ccgcttgcgc ttggtcgatg tggtcataag ttttcaacac caaccatgat
    22381 acgccctctg tgcacggcgg cgtggtcagc gaaccggcaa agcggtagta tttcaaccgt
    22441 ttcggcagta gggtggatgc gtcgaacggt tggttgagtt tcacttttcc tgcggtcatc
    22501 ggcatgacgt tccagatgga agacaggcgg ccgttggttt tgccggcttc atacagcacg
    22561 gctaatacta aaggctgttt gttttcgtct aagtggacga agtgagcttc catcgggaaa
    22621 gtgcggcctt tgatttggtt ttcgctcggc acgtggaagt ggaactgttt cagggtatag
    22681 gtgcggccgt tcacggtcag ggtattgccg ccttcgggat aattgacctg aatggtgtgg
    22741 ccgttgtttt ccacgtcaac catactcggt ttgtaattga ctttgatggc gggcagtttg
    22801 ccggaaacgg tttcggtaat gtttaccgga gattggtttt tgccggtgga gcacaaacgg
    22861 aattcttctg acagattgcc ccagctttcg ggagagtcgt gtccggtata gccccaatgg
    22921 gtgtgattgc cgtgtgcggc ggcggagaaa gcggtacagg ccaacagcag gacagcggtc
    22981 aggcggggta aagtgcgggg gaaacgaggc attgtaagat cctttctatt aaggagtaga
    23041 aaaaaggttg cagtgtgtat gtaaatattt tattttttat taaatgatat ctatattaag
    23101 tgcttaagtc aaacttcccg gtctgaaaac cattagagca atgaaattaa agtgaattta
    23161 tttttctata agtcctgctt tatttggata accatgaggc atggctacct ttatccggaa
    23221 aaatattaat agccgcaaca aagatatttg aatccgattc aaatatcacc agcaagcgta
    23281 gctcggatac ttgtatccga caaagccttt aacatttcca tcgttgcaat ccattgcagc
    23341 aattaccaaa atgtcggatt cgagaatccg atctacggaa aactaaagat tttatgcggg
    23401 ctacggcttg cttgttgtat ttaatcatat ttatttgaat tgaattcttt tattttattg
    23461 tgagatgaaa atagtatttt cgatgagaaa cccatgacta aaatagtata gtctttatca
    23521 ttctttacga agtaacaatt accatatcct gcaatatttt tatcccaaaa atcacaatat
    23581 gattcagtgg taaaatcagt tttagttgat ataaaatcaa aattatttgg gtaagatcca
    23641 tattgctggt aatatttatc tatattttct actaagaaat caatcctttt ttctaacatt
    23701 ttaagattaa tttgcaataa taaaatgtat aaaactatac taattgctat tacttttaaa
    23761 tatgtcattt tattttccag taaatagaag tttagttgtt ttgttaacta tgcctgaatg
    23821 tacacctaag tcttcttttg gtaacgatga ataattagca agccgtagcc tgcatctccg
    23881 ccaaccgcgt gccactatct gcgggcggaa taaactttaa aaccgatgtg caggttaaaa
    23941 cctacaagga tgcgtgcggt atacacgcgt ttttgccgtt caaaccattg tttcaaaact
    24001 tgtattgaaa catcgcccgc tgctttatct tgaaagaatg ccgtctgtaa gcaaacatgc
    24061 ttcagacggc atttttttat ccggcggtct gttatccgat ccgtttgaat accaagtccc
    24121 aaacgccgtg tccgaggcgt ttgccgcgcg cttcgaattt ggtttcgggg cggtagtccg
    24181 gagtgggggc gtagtctgcc gccgtatttt gcaggctgtc gaagctgctg aggacttcaa
    24241 gcatctgctg tgcatattct tcccagtctg tcgccaggtg gatatagccg ccggttttga
    24301 gtttgggcag tagtttggcg atgaaggggg cttgtatcag acggcgtttg tggtggcgtt
    24361 ttttgtgcca cgggtcgggg aagaatatgt ggatgccgtc gagcgagccg tcttgcagca
    24421 tatttttgac aacttctacg gcatcgtgcc gcatcacgcg gatgttttcc aaatggtttt
    24481 cgttgatgag tttgagcagg ttgcctacgc cggggccgtg tacgtcgatg gcgagaaagt
    24541 cggtttcggg caggcggcgg gcgatttctg cgcttgccat tcccataccg aagcctattt
    24601 cgaggatttt aggtcggctg cttccgaaac gggcatcaag atcggccggt gttgcttggt
    24661 agtcgatgcc gaagctgtcc cataaggtat cgatggcgcg ttgctgtgcg gcggtcatgt
    24721 ggctttggcg gaggacgaaa ctgcggatgc tgcgggggtg tccggcttga cggtcgtttg
    24781 gtgtttggtt ttcggcggtg tctgtcataa tgcttctgtg cgcttgtgca atcaggatgc
    24841 cgtctgaatg tgaaaggcct gttcggacgg cgggaaggga aaggggcgat tatacctgtt
    24901 tcgtttcggc agggtaggtg taactctgtt cagacggcat tttgccactg ttgcagcagt
    24961 tcttgcagga cgcttttttg cccgtatccg ttgtttatgg cttcttgcag gtagcggcag
    25021 gctttttctt tgtccgcctg gcaccgtgtc cgctgtaatg gatgcaggcg aggttgtaga
    25081 acgccatact gtctttttgc gcggcggctt gttcaaacca tttgcgggct ttgcagtagt
    25141 cggcggctat gccctgtccg aagtagtaca tactgcccag attggtttgg gctttgctgt
    25201 gtccgagttc tgctgcttct gtgtagagtt tgagcgcggc tgcgtattcg ggcgcgcatt
    25261 gtaatccgta atgcagggct tgggcttttt gataaagctg ttctgccctt tgccgcctaa
    25321 tggcggaatc tttgatgccg tcatattgct cgggatggtt taacgcgctg tcggatatga
    25381 gtttctgata ggcggtaagg ctgccggctt cggctgcctg ccggtaataa tgccgggctt
    25441 tttccgtatc gagggctatg cccaaaccgt agcggcagat gtcgcccggt atccgcaaac
    25501 cttcgggatg gcgttcggcg gcggctgctt cggcatgatg cagggcaagt ttgtggtttc
    25561 tttccagata cttgccttgc aggtagatgt cggccagtgc ggcatgggca tcggggtcat
    25621 tttcctgtgc cgcctgttgg aaccggtaaa cggcttcatc gggagccgta ggtaggatga
    25681 gttcggcaag cggggtgtag gcgggaatgt atccttgttg cgcggcggcg cgcaaatgat
    25741 tgacggcctg tgccgtgtcg gctttcgtcc cttgtccgaa acggtattgc agcccgagtt
    25801 gccaatgtgc ttccggcaag ccttgtgccg ccgctttgcg gtagagcagg tgcgccgttt
    25861 caaaatccgg cggcgtgccg tgccggcagt attgggcgag caggtattgc ccgtggggat
    25921 tgttgttttt tgcgcttgtt ttgcccattc cagggcttct tcaaaatgtc gggacgcgta
    25981 cagaaggtac atcaggcgga cttgcgcttc gggcaggcct gcttctgcca gaggcctgta
    26041 aagcggtatc agggtttccg ggggcgcgcc ttccaattct tggatatatg ccaaattgta
    26101 acgcgcatag ggatgcccct gttcggcggc gatgcggagc atttccatac cttgttcgcg
    26161 gtcttggtgc gggctgtccg cgttcatcag gtgttttgcc aactcgaatg tggcggcggg
    26221 atgatctgcc gaggccgctt ctgtcagcag tcggcggact tcggtaaagt tataatattg
    26281 tttttgagac aggcgttcca aggcctgccg atagggtaag tcgggattgt tcatatcaga
    26341 tcgttttccg ttttgtcgaa tcatcgttat tcgtcatgaa gttaggttta tatcggtatg
    26401 ccggcctgct gtatcatatt tttacaggcg gtttttgctt tggcggcata ctaatgcaga
    26461 tgattataaa tggtttccgc caatgccttg ctgatgcctt ccactttttc caagtcctcg
    26521 cggctggcgg caatcacgcc gcgcagtccg ccgaagcggg taagcagggc ttggcggcgt
    26581 ttgctgccta caccgggaat gtcgccgaga gacgaggtaa cgcgtgcttt gtcgcgtttt
    26641 ttacggtggc ctgtgatggc aaagcggtgt gattcatcgc gtacggtttg caataaatgc
    26701 aaggccgggc tattaggagg caggcggaag agttcgccgg taaaaggcag tatgagttct
    26761 tccatgccgg ctttgcgttc ggggcctttg gcaataccga ctaaagggat gtgcagcccg
    26821 agttcttccc atacggatac ggctatgccg atttgccctt taccgccgtc aatcaacacg
    26881 acatccggcc atttgacgct ttcgccgttg gcttcggctt cctgtatttt gccataacgg
    26941 cgcgtcaaca cttcgcgcat ggcggcgtag tcgtcgccgg gtttggcggt ggtgatgttg
    27001 tagcggcggt attgcgaggg ttggatgttt tgttcgtcgt acacgacgca ggaggcgatg
    27061 gtggcttcgc cttgcgtgtg gctgatatcg aaacattcaa ggcggttgat tccgtctgaa
    27121 ttcatgccga ggattttggc aagttcgtcg atgcggtgtt gttggttgct ttgttgcagg
    27181 tggcgttggg taatcgccat ttgtgcgttt tgttccgcca ttttcaacca aactttgcgt
    27241 tcgcctatgg ttttggtaac gaactgcatc tgcttgccgt gttctccttc caaagcctct
    27301 ttcaacgcat cgggaacggg aaagttgctg atgatgatgt cgggtttgct tttgcccaga
    27361 tagtgttggg cgacgaaggc ttcggcgtaa tcttgaccgt ttggttcggg gtcgtttttg
    27421 gtgtcgggaa agaagctttt gtcgccgacg tgccgtccgc cgcggatgct gacccagtgt
    27481 acgcagacaa ggccgtctga aacggcgagc gcgagcaggt cgatatcgtt ggggttgttc
    27541 gggtttttgc tgtcgatgaa ctggttgctc tgtataatgc cgagtgcttg gatttggtcg
    27601 cgataacggg cggcttcttc aaattgcaga ttcgcggcgg cggtctgcat tttgtgttgc
    27661 agggtgcggg tcagttcgtc ggttttgccg ttgaggaaag tggcggcttg acgcacgctg
    27721 tcgcagtagt cttcttcgct gatgtgtccg acgcagggcg cggtgcagcg tttgatttgg
    27781 tacagtaggc aagggcggtc gcggtgttcg aacacgctgt cttcgcaggt acgcagcatg
    27841 aagacttttt gcaacacttg aatgctgtcg cgcacggcgt tgctgttcgg atacgggccg
    27901 aagtattggt tgggattttt cagcgtgccg cggtaatacg ccatttgcgg atattgatgg
    27961 ccgctgagca tcaaataagg atagcttttg tcgtcgcaaa aaaggatatt gtatttcggc
    28021 gacaaggctt tgatgaagtt gttttcgaga atcagggctt cggcttcgga acgcgtgatg
    28081 gtggtttcga tgtggcgaac ctgtttcacc atcaacgcga tgcgcggcga atggtcgttt
    28141 ttttggaaat agccggacac gcgccgcttg aggttgaccg ctttgccgac gtataagacg
    28201 ttgccgcctt cgtcaaaaaa acggtacacg cccggcagct tgggcaggtt tttgaggaag
    28261 agcgggatgt cgaattgttc cggagaacgg ttttctgtat tcatatcaat catttgaaaa
    28321 acggcttcag acggcatatc ggcagcggca gccttatccg ttcggaaaag ctgtcgggcg
    28381 gttatatgcg gtcaatcgat ttttacccat ttcaggcgca gggcattgcc caataccgaa
    28441 accgagcttg ccgccattgc cgcgcctgct atgacgggat ttaaaaagcc gagcgcggcg
    28501 agcggaatgc ccaatatatt gtagaagaag gcgaaaaata ggttttgctt gatgttttcc
    28561 aacgttgcct gcgatatcag cagggcatcg gcgagctgat tgaccgaatg ctgcatcagc
    28621 gtggcggagg cggtgtgttc ggcaacgtcc gcaccgcctt tcatggcgaa gctgacgttg
    28681 gcggcggcaa gcgcgggcgc gtcgttgatg ccgtcgccga ccatcgccac ggttttgccg
    28741 gcggctttga gtttctgcac ttcggcggct ttgtcgcacg gactcatatt accgaaggcg
    28801 tgtgcgatgc ccagttgttt ggcgacgtat tcgaccgtac tttggttatc gccgctcata
    28861 atatagacat cgatattgtg ttttttcaga cggcctatgg cttcggcggt atcggctttc
    28921 aacgcgtcgg agagtgcgaa tgcgccgatc ggtttgccgt ttacagatac ggtaaccgca
    28981 ctggcgattt cccaaacgcc gtctgaaaac ttcggcaagg tcagttcggc aaattcggct
    29041 ttgcctgatt ttaccaaacc cacgccttcc acttcggcgg taatgcctgc tccgacaacg
    29101 gtttgcgcat tttgtgcagc gggaatctcc aaaccgcgcg cttgtgcggc ggagacgatg
    29161 gcgcgggcga gcgggtgggc ggcgttttgc tcgacggcgg cggcgatgcg gtacaaagcg
    29221 tcttcgtcaa agccgctgtc gggaacgtaa taaacggcgg caacctgcgg cctgccttcg
    29281 gtcagcgtac cggttttgtc caatacgacg gcatcgacgt gggctgcttc ctccattgcc
    29341 gccgcgtctt taaaccaaat gccgtgtttc accgctttgc ccatgccgac cataatcgcg
    29401 gcaggggtcg ccagaccgag cgcgcacggg caggcaatca ccaaaacggc aacggcgtgc
    29461 atcagtgcga ccgtccaatc gcccttaatc agccaagtaa cgataaaagt cagaagcgcg
    29521 atgcccacga cagttggcac aaataccgcc gccgctttat cggccacgcg cgcaatcggt
    29581 gctttactgc cttgtgcttc agagagcgcg ttcatcatgt cgccgagcag ggtttggctg
    29641 ccgagctgcg cggcgcggta caccacgctg ccttcggtca tcagcgcgcc cgccaacact
    29701 ttgccgcccg cctttttctc ttcgggattg gattcgccgg taaggtggct ttcgtccgcc
    29761 caaccgctgc cgctttcgat aatgccgtcg gcagcgatgc gttcgccgtg gttggtgcgg
    29821 ataaggtcgc cgatttgcac ttggtcgatg ggcagttgtt tccattcgcc gttgcgttgc
    29881 acgttgactt gggtcggcgt gagttttagc agtaagccca agctgttcag gctggatttt
    29941 ttggtgcggt gttccaaaaa cttacccagc gacacaaaac cgatcaccat cacgcccgct
    30001 tcaaaataca catgcgccat accgtgcgcc gcatgcgaac tgaaaaacag catataaacg
    30061 gaatacaggt aaatcgacac cgtgccgatg gtaacgagta cgtccatatt cgccagcccg
    30121 cctttaatgc ttgcccacgc gcttttgtaa aacgggattg ccagccaaag ttgcactatg
    30181 cttgccagta caaactgcca tacaggcgga atcatccaat cgtgccgtgt ccaattcagc
    30241 ccttttagca tcatccctac cataccgata aggaacggga tattgatggt cagcaaaagc
    30301 cacaaccgcc agccgatatg gtgttctgct tcaggttgcg gcaatgtatc ttccgttttt
    30361 tccttcgcgc cgtaaccggt tttctcaatg attttggcaa tgtcggcaac cgaggttttg
    30421 ctgccgtcaa acgtaacctg cgcttcctca ctggcaaagt tcactcccgc cgattcgaca
    30481 aaatcttttt tgttcaacac tttttcaatg cgcgaagcac atgcctgaca ggtcatcccc
    30541 tcgatttgga aacggatttt ttgttgcata agtttgattc ccgttttgat gttcagacgg
    30601 cagtcaaggc tgcgggatgc cgtctgaacg ccggtatggt caaaatttct caaacgcgcc
    30661 gataattcgg gtagaatccg aaaacttgac acagcaaagg aaaaacaatg tcccaacacc
    30721 gcaaactgat tattttgggt tccggccccg ccggatacac cgccgccgtc tatgccgcac
    30781 gtgccaattt aaaccccgtc attattacag gtatcgcgca aggcgggcaa ctgatgacaa
    30841 cgactgaagt ggacaactgg cctgccgatg ccgacggcgt gcaagggccg gaattgatgg
    30901 cgcggtttct cgcccacgcc gaacgtttcg gaacggaaat catttttgac caaatcaacg
    30961 ccgtcgatct gcaaaaacgc ccgttcgcac tcaaaggcga tatgggcgag tacacttgcg
    31021 atgccctgat tgtcgccacc ggcgcgtccg ccaaatacct tggtttgccg agtgaggaag
    31081 cgtttgcagg gaaaggcgtt tccgcctgcg ccacctgcga cggtttcttc tacaaaaacc
    31141 aagatgttgc cgtagtcggc ggcggcaata cggcagttga ggaggcactc taccttgcca
    31201 atatcgccaa aaccgttacg ctgattcacc gccgcagcga gttccgtgct gaaaaaatca
    31261 tgattgacaa actgatgaaa cgcgtggaag agggcaaaat catcctcaag ctggaaagca
    31321 acctgcaaga agtactgggc gacgatcggg gcgtaaatgg cgcattatta aaaaacaacg
    31381 acggttctga tcaacaaatt gccgtcagcg gcatctttat cgccatcggg cacaagccga
    31441 ataccgatat tttcaaaggg cagttggaaa tggacgaagc cggctacctg aaaacaaaag
    31501 gcggcacggc ggacaatgtc ggtgcaacca atatcgaagg cgtatgggcg gcgggcgacg
    31561 taaaagacca tacctaccgt caggcaatta ccagcgcggc ctccggctgt caggcggctt
    31621 tggacgcgga acgttggctg ggcagccaaa acatttgaat gccgtctgaa accgaaattt
    31681 tcggcggaat gcaagttttt cgtaccaaat ccttgattcc gtcggcaaac cccaatataa
    31741 tgcctgctat ttgaaccttg ccttctgctt tcgcatggca gaaggctgtt tttaccatcc
    31801 ataaggagat aacatgcgtc attacgagat cgtgtttatc gttcatcctg atcaaagcga
    31861 gcaagtgccc gctatggttg aacgttacaa aaccatgatt accgaggcaa acggtaagat
    31921 tcaccgtttg gaagattggg gccgccgcca actggcttac ccgattaaca aaatccataa
    31981 agcacattat gttttgatga acatcgaaac cactcccgaa gttgttgggg agttggaaac
    32041 cgcgttccgt ttcaatgatg cggtattgcg tcatctgacc atcaaaacca aacacgccgt
    32101 taccgaagcc tcccctatgc tgggcggcga aaaggctaag aacctgttgg tcggcgcggc
    32161 tgaagaagcg gctgcccaat aattgggatt cactaatctt gtttcgcttg ccgcgctgat
    32221 tgaaaaggct ttccctattc gatatacgcc tgccggaatc cctgttttag atattatttt
    32281 aaagcacgaa tcgtggcagg aggaaaatgg gcagcaatgc cttgtccaat tggaaatccc
    32341 cgcgcggatt ttgggtaggc aggcggaaga gtggcagtat cggcaaggtg tatatgttca
    32401 cgtcgaaggt tttttagctc aaaaaagcag acgttccctg atgccgatgc tcaggataca
    32461 aaatattaaa gaatataaag gttaaacgac aatggctcgt caatcattca aacgtagaaa
    32521 attctgccgt ttcacggctg aaaaaatcca agaagtcgat tacaaacaag ttgatttgct
    32581 gaaagacttt atctctgaaa acggtaaaat cattcctgcc cgcatcacag gaacgaaggc
    32641 attctaccaa cgccaattgg ctgttgccgt aaaacgcgcg cgcttcttgg cactcctgcc
    32701 ttataccgac caacacaaat aattttggag tttaaatcat gcaaattatt ctgttagaaa
    32761 aaatcggcgg tttgggtaat ttgggcgaca tcgttaccgt aaaaaacggt tatgcccgca
    32821 acttcttgat tcctgctggt aaggcaaaac gtgctactga agcaaacatg aaagagtttg
    32881 aagcacgccg cgccgaattg gaagccaaac aggccgaaat tttggcagat gcccgagccc
    32941 gtcaggaaaa actggacggt caaaccgtta ccgttgcaca gaaagccggt gtggacggcc
    33001 gcctgttcgg ttccgttacc aatgccgaca ttgctgctgc aatcgttgct gccggcatcg
    33061 aagccgtgaa agcaaatgta cgtctgccga acggtcctct gaaagccgtt ggagagtacg
    33121 aagtggaagt ggctttacac actgatgccg ttgctaaaat tactgttgcc gtcattgccg
    33181 cagccgagta attgaaatta tcagatgccg tctgaaaccc tgccgattat cagaatgtgg
    33241 tttcagacgg catttgtttt gttttccgta tattctgcaa aaatcgggac ggttgttttt
    33301 tatttgcctg aggtctttgc agaaatggtt tgttaacgaa atttgatgca taaaatgtgc
    33361 caaaaaatat tggctgtgtt ttaatatagt gttgacaagc gtacctggtt tcaatatatt
    33421 caataggata ggtctcttcc caaccgaaaa aataattgtc ttttctcttg taaatagttg
    33481 aaatagattg tcaaaataca tcctaaaaaa tttaataact tatatgattg caaaggttgc
    33541 ggcttgtatg ccggtcatta tggcggaaat accgtttggg aggcagtcgc atcgcatgga
    33601 gtcggcacgg caggcgatgc gccgatgcct tcgggcgtga aggcagggaa atcggcagtt
    33661 tggaaaaagg caatggaatc cgcaggtagg tgtcgattgg gtggtgaaag gcaataagtc
    33721 gggcgcacgc tcagactgcc tatccccttg tcggaacggg aggtagggca gctttgtcct
    33781 gactaattgc ttgtgcaaac ggtaagaagg tttttaaagc aggttgtaca gcatttttcc
    33841 ggcaatcaaa agcaacataa tgccgaagga ttctttgagt ttggcagaag aaagtttgtg
    33901 ggcggttttg acaccgagcg gggcaaaggc aatagttgcc gcgctgagga cggcgacggc
    33961 gggcaggtaa aggaagccca gcgacccttc gggcaatcct gcaatattca gaccgttgac
    34021 cagatacgat attgcgccgg agagtgcaat cggccaggca aggccggatg atgtgccgat
    34081 ggctttatgg gcggggaagc cgcagtggat taagaagggg acggaaagtg aaccgccgcc
    34141 tatgccgacc cagctcgaca ttgcgccgaa cagtgtggaa accgcagtca gtccgggcag
    34201 cccgggcagc gggcgggatg ccgtctgacg accggtatgc agtgttttga atgcgacggc
    34261 ggttaaaaac aggatgaaga aaatttgaag cccgaacgcg gggatatatt ttgcggagag
    34321 tgcgccagcg aatacgccga atatcatacc cggcatcatc gcaaatatgg ttttccagtc
    34381 gaccgtctgt tttttgtgct gccccaacat actggaaaag gcggtgaaga ccatgacggc
    34441 gaaggatgtg ccgacggcga ggtgttgcgc gtaaggatgt tgtgccaaac cctgcaaatc
    34501 aagcacccat aaaacgacag ggacaatcag cgtaccgccg cctacaccga acaggccggc
    34561 aataaaacct gccgcactgc ctacggcaag caggattaag ataatgtccc aatgccacat
    34621 ttaatccgcc tttctgtatt ttttccaaaa tcggcgtgcc gccataatgt agccggacag
    34681 gctgtatccg agaaagaaca ggaaaaggac gagcgacggt tcccaagtga ccagaagcag
    34741 cagtaagacg gcaagcagca ttccgacaaa ggggacttgt ctgcggatgt tgatttcttt
    34801 aaaactccaa aaagggattt ggacaatcat cgacaggccg gcaaacagtg tgatgcctaa
    34861 tgcccaccag tggacggcgg ggaatttttc gacgctgtgg ttgacccaaa tcagcccgac
    34921 aatcagcgcg gcggcagtcg gactgggtac gccgataaac cagcgtttgt ccaccttgcc
    34981 gatgagcgtg ttgaacagcg cgaggcgtag ggcggcgcag gcgcagtaga tgaaggcgac
    35041 ggaataaccg attttgccga actgccaaag ctgccatttg taggcaataa gcgcgggggc
    35101 aacgccgaag ctgaccatgt cggcaaggct gtcgagctgc tccccgaacg cgctttggct
    35161 gttggtcagc cgcgccacgc gcccgtccat accgtccagc agcatagaga tgaataccgc
    35221 gatggcggcg gtttcataac gtccgtgcat ggattgggta atggcgtaaa acgcggaaaa
    35281 cagcgcggcg atggtaaagg aattgggcag caggtagata ctgttttgac gcagggagcg
    35341 ttgcggggtg ttggtcgggg tttccatagc tgaagtgtct cacggacgtg gagaatcgat
    35401 aggggattat aacccaaacc attatcgaac aatacgccct ttaggatgaa gccctcggag
    35461 gcggtttgtt tttctgatgg cggatgcttt gcggggcggt ttgactctgc ctgccaccgg
    35521 gtgtgttgtt tcggattttg ccggatggat attgttgccg ggcatttctg cgcttgccgg
    35581 aaggtatcgg agggaaggca tcggaatatg cggcgcgccg ttcaaaccgg ggcggtattt
    35641 ccctattttt tggggggtgc aggtttcaac aaattgaaag atattttttg catttccgac
    35701 cgtatgctga ttttgaagcg ggataaatta taggacaagg tttttcgccc gccgcaaagg
    35761 ttgcccgcaa tgccgtctga aaacaacggc ttgccgctaa ggcggcaata tgaggatttg
    35821 cgctgccgaa accggtttgc tacaacccgg cagtgaaatc tgcagggagg gataggccga
    35881 taaggcggga cttatcttgt tttttgttta taatgcaaca gaatttggag gaatcgtcgg
    35941 gaatttatca tgatgaaaaa gatactggca gtatcggcac tatgcctgat gactgcggcg
    36001 gcacaggctg ccgatactta cggctatctc gccgtttggc agaatccgca ggatgcaaac
    36061 gatgttttgc aggttaaaac cacaaaagaa gattcggcga aaagcgaagc gtttgccgag
    36121 ttggaagcct tttgcaaagg tcaggacacg cttgcgggca ttgccgaaga cgagccgacc
    36181 ggatgccggt cggtcgtgtc gctgaacaat acctgtgtct cgctggcata cccgaaagcc
    36241 ttgggcgcga tgcgcgttga aaacgccgtc gtgattactt ctccgcgttt tacgagcgtt
    36301 catcaggtcg cactcaacca gtgcataaaa aaatacggcg cacagggaca atgcggcttg
    36361 gaaacagtgt attgcacgtc atcttcttat tacggcgggg ctgttcgctc cttaatccaa
    36421 cacctgaaat aaaaaatgcc gtctgaagga tgttcggacg gcatttccat atcgacggtc
    36481 aggattcttt cggatcgggc agcaggctgt tcaacacaat ggcgagtacg gcgcacaagc
    36541 ccacgccgac aaagctgaag ctgcccaatt tgagtgtcat accgccgatg cccgtggtca
    36601 gtaccgagct gacgatgacc aggtttttcg gcagcatcaa atcgactttg gcatcaatca
    36661 gggttttgat gcctaaagaa gcaatcgtgc cgaacagcag cagcataatg ccgcccatca
    36721 cgggcatcgg aatggaagcc aaaaacgcat tgaatttgcc gaaaaacgcc atgcagacgg
    36781 caaaaaccgc cgcccaagtc atgatgacgg ggttgctgtt tttggtaatc atcaccgcac
    36841 ccgttacttc gccgtaggtt gtaaccggcg ggccgccgat cagacctgca acgcacacgc
    36901 ccaagccgtc gcccgcaagg gttttgtcca agcccgggtc ttttgtgtag tcttttcccg
    36961 ttacattgcc gattgccatg atgccgccga tgtgttcgat ggcgggggcg acggcaacgg
    37021 gcagcataaa cagtgccgcc tgccagttga cctgaggcgt ttcaaaatgg ggaacggcga
    37081 accagggcgc gtgtgcaatg cttgtcgtgt ccaccagtcc catcagcagt gccaaaacat
    37141 aacccgaagc gacaccgatc aagatgggaa tcagcttcat catcctgctg ccgaaaaccg
    37201 atacgatggc ggtaacggca aaggtaaagc cggaaagaat cagcgaatcg gtatagtcga
    37261 tgacctgttt gccgtcagcc tgacccattg ccatgctgct tgccgccacg gcaacagaca
    37321 gaccgatgac catgatgaca gggccggtga cgaccggcgg cagcagtttg tgtaccgccg
    37381 ccagtccgcg ccaacggatc agcgcggcaa acacaaaata cataaagccg gcggcaaaca
    37441 gcccgaacat ggtggaaggc agcccccatt cgccgacgga gtagataatc ggcgcgataa
    37501 aggcaaacga agaaccgaga aaaatcggta ctttgcgttt ggttgtgatt tggaacagca
    37561 gcgttcccaa gcctgcgccc aaaagcgcaa gagccggatt cagaccggtc agcaggggaa
    37621 ccagcaccat tgcgccgaat gccacaaata aaatctgcgc gccggaaacg gcaagtttca
    37681 gttggttcat accatcttct ttcaatcggt tcaaacggca cggattatac tggtttgccg
    37741 aggggctgcg taaaaataca tacgtcgggc aaagatatgc cgtctgaaag gcaacgtctg
    37801 tctgttgcag gatgaaagcg ggagaaatgg agtataatcc gacactttcc atattcaatc
    37861 cgcaagccgt cccgaaccct tatttcagac ggcattttcc ggtaatacgg catgacagaa
    37921 aaatcccata aaaaaacagc aaaaggcagg gcgggaagcc cgtccccaac gtccgcacgc
    37981 aacaagaaag ccgacaacgg cgcgcggggc aataaagttt ccgagcggct caaggcggtc
    38041 aaagagctgc agaaaactga agccaaaaag gcgcgtcccg aacatgtcgt caacctgatt
    38101 gccgacgcgc tgtggctgat gggtttggcg gcaaccctgt atttggcgat ttccctgatc
    38161 agtttcgata tgggcgatcc gtcttggtcg cacagttcac cggttgtgga agatgccgcc
    38221 aattggggcg gactgttcgg cgcgtatgtt gccgatgtcg gctattatct tttcggctgg
    38281 tcgttctggt ggtggatagc ggctgcctgc gtcgtgctgt ataaaaattt ccgtctgcac
    38341 gcaaaacaga cggaaaacga ggcatacaac cacaaaatcg ctgccgccgc gctgtttgtc
    38401 ctgacggtct tcagccccgt cttggagtat tttgtgctgg gcggaaaata tgccgactcc
    38461 ctgcctgtcg gagcaggcgg tatggtcggc atacgtgtcg gcgcagtgtt tgcgtggctg
    38521 ttggggaaat cgggcagcct gctgattatc ctggttgttc tgctgttgtc gttgtccctg
    38581 ctggtgcaga tttcatggct ggaatttttg aacggtgcgg gcagggcggt tcaaaaccgc
    38641 ctgagtgcct tatccggcaa ggtcatggct ttgagaaaac gccggccgaa taccaaaaca
    38701 gacggtgtcg atacccaaaa tacacggcgc atggtgaaag aagccaaaaa catcactgcc
    38761 aaacccgttg ccttgcccga aggcagcagc agcaaccgca aatccgtcgc ggtttccgtt
    38821 gcgccgccgc ctaaaatcca accttcgctg tttgaagaca atgaagtaca gcagaacggc
    38881 gaataccaca agcctacatt gaacctattg cggattcccg acagcgagcc ggtcagcatc
    38941 aatcccgccg aattggagcg cactgccgaa ctaatcgaat ccaaactggc agaattcggc
    39001 atcggcgtac aagtcgtatc cgcgacatcc ggccccgtca tcacgcgcta cgaaatcgaa
    39061 cccgcgcaag gtgtcaaagg cagccaaatt gttgccctgt cgaaagattt ggcacgctct
    39121 atgtcgctgc aatccgtgcg tatcgtcgaa accattgcgg gcaaaaacac gatgggcatc
    39181 gagttgccca acgacaaacg ccaagacgtg atgttgagcg aaatcttgtc ctcgcccgtg
    39241 tttgccgaag ccaaatccaa gctgaccgtc gcgctgggca aagacatcgc cggtacgccc
    39301 gtcgtcggcg acttggcgaa aatgccgcac cttttggtcg ccggcatgac cggttcgggc
    39361 aaatccgtcg gcgtgaacgg catgattatg tctatgcttt tcaaagctac gcccgaagaa
    39421 gtccgcttca ttatgataga cccgaaaatg ctcgagttga gcatttacga cggcattccg
    39481 cacctactct gtcccgtcgt taccgatatg cgcgaagcag ggcaggcgtt gaactggtgc
    39541 gtcgccgaaa tggaaaaacg ctaccgcctg ctctcccacg ccggtgtacg caatctggaa
    39601 ggcttcaacc aaaaagtcga acaagccaaa gcggcaggca agccgctgct caatccgttc
    39661 agcctgaacc tcgacgaacc tgagccactg gaaaaactgc cgatgatcgt ggtcgttatc
    39721 gacgaacttg ccgacctgat gatgaccgaa cgcaaagctg tcgagcagca aatcgcccgc
    39781 ctcgcccaaa aagcgcgcgc cgccggcata cacatgattg tcgccaccca acgcccaagc
    39841 gtcgatgtcg taaccggtct gattaaagcc aacatcccga cgcgtatggc gtttaccgtg
    39901 caaagcaaaa tcgacagccg taccatcctc gaccaaatgg gcgcggacga actgctcaaa
    39961 tatggcgatt cgctgttcct gcaaccgggc agtgccgaac cgacccgcct gcaaggcacg
    40021 tttgtttcag acggcgaagt gcatcaagtc gtcaactacg tcaaatcgca agccccagcc
    40081 gactatattg aaggtctgct cagcggcgaa gccgcgctgg aaactgccaa tatcgttaat
    40141 ccgaatgcag acagcgacga attattcgat caggcagtcg cctatgtttt ggaaagcaaa
    40201 aaaacctcca tttcgtcttt gcagcggcag ttgcgtatcg gctataaccg cgcggcaaac
    40261 ctgatggagg cactggaaaa tgcgggtatc gtttctccct ccgacctcaa cggcagccgt
    40321 aaaattttgg cgcacaagga ccatttatag cccgtatcgc
//
LOCUS       DBJGGI010000011        39437 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.011, whole genome shotgun sequence.
ACCESSION   DBJGGI010000011 DBJGGI010000000
VERSION     DBJGGI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39437
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.011"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(1..423)
                     /locus_tag="ACSIGJ_000587"
                     /pseudo
     CDS             complement(1..423)
                     /locus_tag="ACSIGJ_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            630..2636
                     /locus_tag="ACSIGJ_000588"
     CDS             630..2636
                     /locus_tag="ACSIGJ_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HHQ1329539.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFNPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TAPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVGRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGGIIALMALLKIRIGTLDLSPFLASLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLSKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRLPILDAHTAAYQFKSIDIINHPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGFGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKGQGHPPSDKP"
     gene            complement(2759..3850)
                     /gene="mltB"
                     /locus_tag="ACSIGJ_000589"
     CDS             complement(2759..3850)
                     /gene="mltB"
                     /locus_tag="ACSIGJ_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HHQ1329540.1"
                     /translation="MEKRKILPLAICLAALSACTAMEARTPRANEAQAPRADEMKKES
                     RPAFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSQAEWQDFFDKAAYKADIVKIMH
                     RPSTSRPWYVFRTGNSGRAKFHGARRFYAENRAVIDDVAQKYGVPAELIVAIIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNVGDVAASVANYMKQHGWRTGGKMLVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGETLADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(3998..5425)
                     /locus_tag="ACSIGJ_000590"
     CDS             complement(3998..5425)
                     /locus_tag="ACSIGJ_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HHQ1329541.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEGK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGGEAQVAQGLDMIFKGEGGGLGVTEPETSGAAIAREMQSCYEYTDEQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSRGDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILDNLNRYVRNDIRFV
                     DYERREIQRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHPAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGIKLDKN
                     QTLLDRLQTDVRFAAVARDYALPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     EEHEDTTAFLLNDIRKDILDCRYCG"
     gene            5610..9314
                     /gene="mfd"
                     /locus_tag="ACSIGJ_000591"
     CDS             5610..9314
                     /gene="mfd"
                     /locus_tag="ACSIGJ_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HHQ1329542.1"
                     /translation="MTCPIPKPREKSRWFNLSQGSLPLALARYLPHKRLKAVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSETPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWNDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKAHSLPDLAVNRQSDEPLQALKDFQTAFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSSSQQQTVPASEGEGKAVTDQTE
                     FSASATNPLPSPLPQEREQSAAAVSDGLKAAAVSTESSLYLVASDLHGQTRQQSAPSP
                     VGEGWGEGKAVAAQSAIAVITESDLYQYVARSRVHNRRKKHAAVSDGLLRDLAEINIG
                     DPVVHEEHGIGRYTGLVTMDLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHE
                     SVALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAQRAAQSGHKFEINESDYQAFADG
                     FGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAV
                     LAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKATLEGMADGTVDIVIGTHK
                     LVQDDIKFKNLGLLIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGL
                     RDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRER
                     LETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIIN
                     RADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLA
                     MQDLEIRGAGEILGEGQSGEMMQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEI
                     KLHSPALLPEDYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLPEQPVKTLIE
                     SHHLRLAAKELGIDAIDATSEAVTVTFGKHHCIDPTGIILLIQTDKKYRLAGADKLRF
                     AAEMENIEVRINTVKTVLKTLQGKRLPKGN"
     gene            9540..9842
                     /locus_tag="ACSIGJ_000592"
     CDS             9540..9842
                     /locus_tag="ACSIGJ_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2004 domain-containing protein"
                     /protein_id="HHQ1329543.1"
                     /translation="MDAFAAFLSELEKADDAARAALTEYLKRDSRYIDFHTEDTQTSR
                     DAAKFVRTMQLIYISLWAKNPAFAVMDYMPANMESDEILAVKLHLDGSIFSIDWES"
     gene            10004..10387
                     /gene="panD"
                     /locus_tag="ACSIGJ_000593"
     CDS             10004..10387
                     /gene="panD"
                     /locus_tag="ACSIGJ_000593"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HHQ1329544.1"
                     /translation="MFRTILGGKIHRATVTEADLNYVGSITVDQDLLDAAGICPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYIQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            10409..11251
                     /gene="kdsA"
                     /locus_tag="ACSIGJ_000594"
     CDS             10409..11251
                     /gene="kdsA"
                     /locus_tag="ACSIGJ_000594"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HHQ1329545.1"
                     /translation="MDIKINDITLGNNSPFVLFGGINVLEDLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFREAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLENFLIRIKALDDLIKSQPI
                     LTIE"
     gene            11266..11706
                     /locus_tag="ACSIGJ_000595"
     CDS             11266..11706
                     /locus_tag="ACSIGJ_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HHQ1329546.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGTVLAAAFAALTRRQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSVAGGSFGMWHGISSMTFMATALLSAVLSWRLSGKEAV"
     gene            11753..13039
                     /gene="eno"
                     /locus_tag="ACSIGJ_000596"
     CDS             11753..13039
                     /gene="eno"
                     /locus_tag="ACSIGJ_000596"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HHQ1329547.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEAAEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGKKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKCNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAAYYPGKAAFYQLGK"
     gene            13207..13485
                     /gene="ftsB"
                     /locus_tag="ACSIGJ_000597"
     CDS             13207..13485
                     /gene="ftsB"
                     /locus_tag="ACSIGJ_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HHQ1329548.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(13526..13816)
                     /gene="yfaE"
                     /locus_tag="ACSIGJ_000598"
     CDS             complement(13526..13816)
                     /gene="yfaE"
                     /locus_tag="ACSIGJ_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I ribonucleotide reductase maintenance
                     protein YfaE"
                     /protein_id="HHQ1329549.1"
                     /translation="MARIGTNKGLFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLAGEDEGLSDSLLK"
     gene            complement(13937..15070)
                     /gene="nrdB"
                     /locus_tag="ACSIGJ_000599"
     CDS             complement(13937..15070)
                     /gene="nrdB"
                     /locus_tag="ACSIGJ_000599"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HHQ1329550.1"
                     /translation="MSYSTFPKTKNDALKEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWVETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDPEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLSAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(15383..17662)
                     /gene="nrdA"
                     /locus_tag="ACSIGJ_000600"
     CDS             complement(15383..17662)
                     /gene="nrdA"
                     /locus_tag="ACSIGJ_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HHQ1329551.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAAEGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYNHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKEVRLGYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVSGLYEAFFADQDEFERLYTKYEQDPNIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPDGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMDRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQAISANTAYDPGKFEGNKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            18193..18504
                     /locus_tag="ACSIGJ_000601"
     CDS             18193..18504
                     /locus_tag="ACSIGJ_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HHQ1329552.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRHTKHISKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRTLPYAEQKIQCRAAYEAFDDFDGGRFR
                     R"
     gene            complement(18554..18703)
                     /locus_tag="ACSIGJ_000602"
                     /pseudo
     CDS             complement(18554..18703)
                     /locus_tag="ACSIGJ_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18767..19534)
                     /locus_tag="ACSIGJ_000603"
     CDS             complement(18767..19534)
                     /locus_tag="ACSIGJ_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HHQ1329553.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPKSRNRAV
                     IALGKGALAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVAGDADVAV"
     gene            complement(19622..20449)
                     /gene="mutM"
                     /locus_tag="ACSIGJ_000604"
     CDS             complement(19622..20449)
                     /gene="mutM"
                     /locus_tag="ACSIGJ_000604"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HHQ1329554.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVILRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEERHPLLEKLGPEPLSEAFCTDYLYAGLKAQKRAV
                     KLALMDNTVVVGVGNIYANESLFRAGISPHRPANRLKKKECAVLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCLRCGGLVVKETLGQRGTFYCTNCQK"
     gene            complement(20502..21167)
                     /locus_tag="ACSIGJ_000605"
     CDS             complement(20502..21167)
                     /locus_tag="ACSIGJ_000605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHQ1329555.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFNRYVASYRFSGMCAVQVGGQWL
                     SLSEDIVCVPRDMSMSAENMALADVFADMLLLPHTLECGVPSQILSEAHRILKPSGRL
                     MLTGFNPYSLWGFCRWFDGVRLPEKRFCLPLPELKRQLADAGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYWGGKALAAGAAR
                     VAD"
     gene            21346..23322
                     /locus_tag="ACSIGJ_000606"
     CDS             21346..23322
                     /locus_tag="ACSIGJ_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HHQ1329556.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQVGLAIMRLNSSILD
                     LPPTKQYFQSGSLWDELRQGFRMGEVNPELVRRHESKFIASRSYFDRVVNRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAVNRARDQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPGRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKNKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLHTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVSIARIQPAAAQTADITVAPLPQETVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQVIPPNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAETSPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            23390..24082
                     /locus_tag="ACSIGJ_000607"
     CDS             23390..24082
                     /locus_tag="ACSIGJ_000607"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HHQ1329557.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIRGGYVSINGTCMDDTDADIDSS
                     YVETLDIDGEAVTVVPEPYFYILLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVPLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMVAAAGNRAQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            24557..25927
                     /locus_tag="ACSIGJ_000608"
     CDS             24557..25927
                     /locus_tag="ACSIGJ_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HHQ1329558.1"
                     /translation="MSSPSNTNRQTWSSRLTYILTVAGATVGFGATWRFPYLVGENGG
                     GAYVFLFCIAMLVIGIPMILVENVIGRRKGVNALDAFGGPMNGKPVAKIWKLVGRMGL
                     LGAFGIMAYYMVLGGWVISYIVNIIGGNLNISSPVDGVVTKGFFTEHIENSPWEIAFY
                     TLLFVAVNQWILVKGVIGGIEKTAKYLMPLLFLFLIAMVVRNVTLPGAMEGVAFYLKP
                     DFSKITAELFVFVLGQVFFALSLGFGVMITLSSYLDKNENLVQTAVITAITNTIIAVL
                     AGFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSHMWAGPVFAVIFFSLLLIAALTTSLT
                     IYEVLITTIQEKTKIRRTAAITIVLSVIFVFGNIPSILSYGPWKDVSVFGKNIFDAFD
                     YISGNILFMLTALGSALFVGFVMKDEAKEELLYKGNHTTVNIWFAYVKYLVPLVILLI
                     FVSNLF"
     gene            26160..26816
                     /gene="cmk"
                     /locus_tag="ACSIGJ_000609"
     CDS             26160..26816
                     /gene="cmk"
                     /locus_tag="ACSIGJ_000609"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HHQ1329559.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVEWHDEENVSALAKKLPAVFSGNRILLDGEDVSDGIRTEAIGMGASAVAQWP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGFTFERILSDIETRDEADRNRKVAPLKQQSDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            26969..28663
                     /gene="rpsA"
                     /locus_tag="ACSIGJ_000610"
     CDS             26969..28663
                     /gene="rpsA"
                     /locus_tag="ACSIGJ_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HHQ1329560.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QATRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            28674..28988
                     /locus_tag="ACSIGJ_000611"
     CDS             28674..28988
                     /locus_tag="ACSIGJ_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HHQ1329561.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(29184..29591)
                     /locus_tag="ACSIGJ_000612"
     CDS             complement(29184..29591)
                     /locus_tag="ACSIGJ_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HHQ1329562.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLYLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            29714..30851
                     /locus_tag="ACSIGJ_000613"
                     /pseudo
     CDS             29714..30851
                     /locus_tag="ACSIGJ_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
     gene            30860..31687
                     /gene="fghA"
                     /locus_tag="ACSIGJ_000614"
     CDS             30860..31687
                     /gene="fghA"
                     /locus_tag="ACSIGJ_000614"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HHQ1329563.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPDNPENQPLG
                     VIYWLSGLTCTEQNFITKSSFRRYAAEHQVVVVAPDTGPRGEQVPNGAAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEEHFPTNGKRSIMGHSMDGHGALVLALRNRE
                     HYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTKDFIETCRAANQPIDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            32217..33368
                     /gene="zapE"
                     /locus_tag="ACSIGJ_000615"
     CDS             32217..33368
                     /gene="zapE"
                     /locus_tag="ACSIGJ_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HHQ1329564.1"
                     /translation="MNNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHRRLKALKSESNPLKSVAAEITKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSGFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRTIWFDF
                     RALCFSPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
     gene            33427..33852
                     /gene="ndk"
                     /locus_tag="ACSIGJ_000616"
     CDS             33427..33852
                     /gene="ndk"
                     /locus_tag="ACSIGJ_000616"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HHQ1329565.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPTEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            33996..35090
                     /gene="rlmN"
                     /locus_tag="ACSIGJ_000617"
     CDS             33996..35090
                     /gene="rlmN"
                     /locus_tag="ACSIGJ_000617"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HHQ1329566.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQASIEIPKLMMSQESSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGVNDKAQHAY
                     ELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRIFRDILQQAEFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            35093..35854
                     /gene="pilW"
                     /locus_tag="ACSIGJ_000618"
     CDS             35093..35854
                     /gene="pilW"
                     /locus_tag="ACSIGJ_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HHQ1329567.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSNPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNVQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            35870..37135
                     /gene="ispG"
                     /locus_tag="ACSIGJ_000619"
                     /gene_synonym="gcpE"
     CDS             35870..37135
                     /gene="ispG"
                     /locus_tag="ACSIGJ_000619"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HHQ1329568.1"
                     /translation="MNTLQRRKTHQVLIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLVSSAPKPPEEVMKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVHDLIQVYRE
                     LGSRCVYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGNNIASEFL
                     AIVEEYVKTNYGKNSSKRNKGKVIPIQSL"
     gene            complement(37189..37803)
                     /gene="clpP"
                     /locus_tag="ACSIGJ_000620"
     CDS             complement(37189..37803)
                     /gene="clpP"
                     /locus_tag="ACSIGJ_000620"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HHQ1329569.1"
                     /translation="MSFDNHLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQVLENRASLRL"
     gene            complement(37899..39209)
                     /gene="tig"
                     /locus_tag="ACSIGJ_000621"
     CDS             complement(37899..39209)
                     /gene="tig"
                     /locus_tag="ACSIGJ_000621"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HHQ1329570.1"
                     /translation="MSVTVEILENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGYPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVDREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGAGQMLPEFEAGVVGMKAGESKDVTVNF
                     PEEYHGKDVAGKTAVFTITLNNVSEPTLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALIKAVELKVPVALVNEEAARLANEMKQNFVNQGMTDAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YADTSRLQAPTSLAVESNVVDFVLGKAKVNKKALSFDEVMGAQA"
BASE COUNT         9962 a        10900 c         9401 g         9174 t
ORIGIN      
        1 ctgcccgggc tatcggcata aacaataccg tccggcatga ttttcttttt gacggcaggg
       61 agcaacgttt caggctcggc atcgtccgcg gcaacggtat aggcccgccc gttgcgtttc
      121 ggaatgccga agacaaccgc ttttcctacc gcgccgcgac cgcgtccgcc tttacgccgt
      181 acgccgaaac agcttccgcc cggcccggcg gggcccccga aaacctcatc ggcagccaag
      241 gccaaacgat ggttggcaac cgtgcggatt ttacggtaaa acagtgccgc cgaatcggga
      301 tggataccca aaatatcggc ggcagaacgg gcggtaactt ccggtacaaa agaacggagc
      361 ggttcttttt gtacttcttt ctttaatttg cagtgtgtta tcttcatatt tcgagggtaa
      421 catatctgct aatctagtac agacccgccg aaattgttat aatcgaaaaa attaaaaatc
      481 aatacggatt attctatcag attgatttat cgatatttat tatatcatta atatagtttg
      541 atttcaaacc ggcggaaaat gcgccgcccg ccgcagatgc gccctccccg ccatcggggc
      601 gtttaacgcc ggattgccga tgcggtacaa tggcagatat gaagaaaatt acccctcaaa
      661 acctgcgtcc cctgctttcg gaaagcttgg gacataccga ttttgtcaac gtcctcaacg
      721 cattgattaa atttttgcgc cgtggcggca aaaaatgtgc gggggagcgt ttcgacctga
      781 ttatcgacac attcaaacaa gacagggaat tactgtcccg cttcagccgg tgtttttaca
      841 tctggctcgc gcaaatacac atttatccgg cactcatcaa actcggcatt ttctcgcgcc
      901 acagctttgc ccgggaaatg ggcatacgca tatacgaacg cttcaacccg tcatataaag
      961 attttgccaa cttgggcgaa gtcttccttt atcttttcca ttccgaaaac gacgacaaat
     1021 ggctgcaaac gctcaatatc cgccaatggc tggttttata cgaactcatc cgtagccacg
     1081 ccgagccgtc caaattgcag acggcgggca tccgccttgc cgatgcgcgt ttgcgcgcca
     1141 tcgaaatgct gtctgtctgg acggcatccg aagccatcga acccgatctc atccgtatcg
     1201 ccccgcgcct gctggaagcc gattcttcct tcgtcgccct ccaacgcgaa accgccaaac
     1261 tggtcgaaca ctaccgcaac ggcaccgcgc cttacgacac cgcccacctc gaagtgatgt
     1321 tcgaccaatg tttcagccag attgactatt tgcgtcgcaa agggacgggc gccggctccg
     1381 gttcgtcggt caaagtcgcc cacctgctcg aacgactccg gcagaccgta ggccgtctga
     1441 agctgctcac agacatacaa accggtgccg gcaacagcaa ccgcctgacc atcgccctga
     1501 tgaactccct catctacgcg gcggtcgaac aatacagcac ccgccacctg cgccgcagca
     1561 gcatccgtat gctcgcccgc agcattaccg aaaacaaaag ccaccacggc gaacactaca
     1621 tcacccgcaa ccgcaaagaa tatttcaaaa tgttctactc ggcagcaggc ggcggcggca
     1681 tcatcgccct gatggcgctg ctcaaaatcc gcatcggcac actcgacctc agtcccttcc
     1741 tcgcttcctt gtcggctggg ttcaactacg gcatcggctt tatgatcatc catatgctgc
     1801 actgcaccgt cgccaccaag cagcccgcga tgactgccgc cagctttgcc gaacaggtcg
     1861 atctcaacga aggcggcaaa gcggtggaca acaaactctc caagctcctc atcgacgtat
     1921 gccgctccca aagcgtcgcc gtcttcggca acgtttccat cgccatcctt ttggcgtgcg
     1981 ccatatcgtt cggctatgcc catctgtacc ggctgcccat actcgatgcc cacaccgccg
     2041 cctaccagtt caaatccata gacatcatca atcatcctac cctatggtat gccgctattg
     2101 caggcttgtg gctgttctgc tccggcatca tcgcaggttt tttcgacaac cgcgccgact
     2161 acctcaacct gcgccaacgc ctgcccttca atcccttgct gcgtaaaatc atgcgccccg
     2221 ggccccgccg cgtcctcgcc gcctacatcc acaaacacta cggctcgctg gtcggcaact
     2281 tcatcttcgg aatgctcttg ggtatgaccg gctatttcgg acacctcctc gggctgccgc
     2341 tggacatccg ccacgtcgcc ttttcctccg ccaacctcgg ctatgccgcc gtcagcggca
     2401 acgtcggctt tggtacattc gtactcggca ttttcagcgt cctcgccatc ggcttggtca
     2461 acctctgcgt cagcttcagc ctcgccctct tcgtcgccct gcgctcgcgc ggcacaaaaa
     2521 tcggcagcat ccgcaacctg atcaaaagtt tttggaatca gattaaaagc aatccttgca
     2581 tacttttcct cccgcccgcc aaaggacagg gacatcctcc ttcggacaag ccttgaccgg
     2641 caatgccgtc tgaaacggga ttcgccccga ataccgccct gatgcgggaa atccccataa
     2701 aaggatgcaa aaatgccgtc tgaaccgaaa cgtggttcag acagcatttt aaaaaacatt
     2761 acaatcccgg gccgccgagc gaattggcaa tgtccctgac cgccgttaca tacatccggc
     2821 tgtggttgta ctgccatacc gtataaaaat tgttcaagcc caaataatat tcaaacacgc
     2881 cgggtgcggt ttccagtttg aacaaaaccg ccttttcatc atctgcgagc gtttccccgg
     2941 ggatgatgcc gtacgccttc aaatccgcca ccgtccgcgt cagggcggtt ttttcgccaa
     3001 tgattgcctg aacatccgca cccggcgcca acgtcgccga caccaacatt ttaccgcccg
     3061 tgcgccaacc gtgctgcttc atataattgg caaccgatgc cgcgacatca ccgacgttgc
     3121 cccatatatc ccgatgtccg tccccgtcat aatccaccgc ccatttccgg tagctcgaag
     3181 gcataaattg cggcataccc attgcgcccg catagctgcc cttaaaggcg aaaacatcgc
     3241 cgccttcttc ttttgccagc tttaaaagct cgaccaattc tttttggaaa aacccggcgc
     3301 ggcggggata atcaaagcct aaggtcgcca atgcgtccgc cacacggaag ctgcccgtat
     3361 ttttgccgta attcgtttca atcccgataa tcgccacgat aagctcggca ggcacgccgt
     3421 atttttgcgc cacatcatcg ataaccgcgc ggttttccgc ataaaacctg cgcgcgccgt
     3481 gaaatttcgc cctgcccgaa tttcccgtgc ggaacacata ccacggacgc gatgtggagg
     3541 gtcggtgcat aatcttgacg atgtccgcct tgtaagccgc tttgtcaaaa aaatcctgcc
     3601 attccgcctg ggaaaaatcc cctttcccga cttcatcgtc cacaaaacgg cggacatttg
     3661 cattggcggc aaacccgctg tcggataccg gtacggctgc cgcgtcaaac gcggggcggc
     3721 tttctttttt catttcatcc gcgcgggggg cttgggcttc atttgcccgg ggtgtgcggg
     3781 cctccatcgc cgtacaggca gacaaagccg ccaaacaaat tgccagcggc agtatttttc
     3841 tcttttccat aaacacattc cgaacaaata gggtaagtgg gaaagcggca caagggtgag
     3901 gcgcgaaatg cggtatccgc cgcaatcggc ggttttgcag aatgccgcgc gttgcctttt
     3961 gcgccgcccg aaatctgcct gccgccgtat ttgcccgcta tccgcaatat cggcagtcca
     4021 atatatcttt gcggatgtcg ttcagcagga aggctgtggt gtcttcgtgt tcctcctgta
     4081 cgaagacaaa gagtcgggcg atgattttgc cgatgaagac tttttgcagg gcgcaggcgt
     4141 cggtcagggt gtgttcctgc aggaagctgc ggatgtcttc gggcaaagcg tagtcgcggg
     4201 caacggcggc aaagcggaca tcggtttgca ggcggtcgag cagggtttgg tttttgtcca
     4261 acttaatgcc tgcttctttt tcttcggcgg tggcgcggac gaactggtcg taaatttcgg
     4321 cataaagcat atcgttcggg ccttcaaaaa tcgtgaaggg gcggatatcg atggcgatat
     4381 tgccggctgg gtgtccgcgt tcaaaaccct tcgcgcccaa gagtttttgc aacatttgcg
     4441 cggcggcgta agtgtattcc gtggcgaggg ttttgacgat gttcgcctcc atcaattgat
     4501 gggcgacggg cgcgacgggc gaaacggaat ggcagacgta gcggtaaagg atttcggaaa
     4561 cctgatggcg gcgttggatt tcgcggcgtt cgtaatcgac gaatctgata tcgttgcgga
     4621 catatcggtt caggttgtca aggatgtatt ccataatgcc gtgcgtcatg ccgatcagtt
     4681 gcaggcggct gcggataaag atgttttgga acgcgcgcaa accggcagcg tcgccccggg
     4741 agagtttcat cacggcggtt gcaggcattt cggcatcgat gcggttgacg gcgtaacgga
     4801 cggcgcgcag gccttcggat gcgagggttt cgcagcggat gtatgttttg gggacgagca
     4861 gcaggtcgat gactttggcg agtttgccgt ttttgcgctc tttggcggca acgaggagga
     4921 agtcgctttg cgaattgccc tgccagtatt tcgcggcgtt gacgtaaatg gtttgttcgt
     4981 cggtatattc gtagcaggac tgcatttcgc gtgcaatcgc cgcgccggag gtttcgggtt
     5041 cggtaacgcc caaaccgccg ccttcgcctt tgaaaatcat gtccaaacct tgtgcgactt
     5101 gcgcttcgcc gccgaactct tgcagaggct gcaacaccag cgcgccttcg atgccggtac
     5161 gcagcgtaac gggcacgccg taatgccccg caatccttag gacttcttgg atttcaaact
     5221 gactgccctt gcgcccgccg tgttttttgt cgaggaaggg caacagcaaa cccgcctgct
     5281 tcaaggcaag ccatttgcct tcgggcaggt atcgcatcag gtcgatgccg tctgaaaaaa
     5341 tgcggcggaa tgcggattcg atgtgcttta aaaaagcagc cgtgtccatg gttgacggct
     5401 gcgcgctcgg ttcggtgtgt atcatcggct tcctctgtcg gttcccatta atcggcggcc
     5461 ggtcaaaccg cctgccacag tttagagtta attttctaaa ctttaccaca aagtgcaccg
     5521 ggcaacaatc cgccggcctt tcagacggca tcgcgcaccc tcacgtgcta aaatgaccgt
     5581 ttgcatcact gtccgccgat tgccgcacta tgacctgccc catccccaaa ccccgtgaaa
     5641 aatcccgttg gttcaatctt tcgcaaggct cgctgccctt ggctttggcg cgttatttgc
     5701 cgcacaagcg gctcaaagcc gtgctgaccc aagatgcgga acaggcgttg cgccttcaga
     5761 cggcatggcg gtttttccgt ccgcacgaca cggcggtgtt cctgccggac tgggaaacgc
     5821 tgccttacga gcgtttttct ccgcatcagg atttggtgtc ggagcggctg tcggcgttgt
     5881 ggcagattaa aagcggcgcg gcggacgtgt tgttcgtacc ggttgccacg gcgatgcaga
     5941 agctgccgcc cgtgccgttt ttggcggggc gcacgttttg gctgaaaacg gggcagactt
     6001 tggatatagg ccgcctgaaa accgatttgg tggatgcggg ctacaatcat gtttcccacg
     6061 ttgtcgcggc gggcgagttt gccgtgcgcg gcggcattgt cgatttgttc ccgatgggca
     6121 gcgaaacgcc gtaccgcatc gatttgtttg acgatgaaat cgacagcatt aaaaccttcg
     6181 ataccgacac gcagcgcacc atttcccccg tttccgaaat ccgcctgctg ccggcgcacg
     6241 agttccccac cgacagcgag gcgcaaaaaa tcttccgcag ccgcttccgc gaggaagtcg
     6301 acggtaatcc gaacgatgcc gccgtgtaca aagccgtcag caacggccac ttcggcgcgg
     6361 gcgtggaata ttatctgccg ctgttttttg aaaacgagct ggaaacgctg tttgactata
     6421 tcggcgaaga tgcgctgttt gtctctttgg gcgatgttca tgccgaggca aaccgctttt
     6481 ggaacgacgt taaatcacgt tacgccatgg cgcagggcga cgaaacctat ccgcctttgc
     6541 ttccacagca tttgtatctc tctgccgatg tgttcgcagg ccgtctgaaa aactacggac
     6601 aagttctgcc tgatgtttcc ggcaaggcac actccctgcc cgaccttgcc gtcaaccgcc
     6661 aatccgacga gcctttgcag gcattgaagg attttcagac ggcctttgac gggcggattt
     6721 tgctgtgtgc cgaaagtttg ggacggcgcg aaaccatgct cggtttcttg cagcaaaacg
     6781 gtttgaaagc caaacctgtg tccgactggc agggcttctt gtcggcgcac gagccgctga
     6841 tgattacggt cgcgccgttg gcttacggat tcaaactggg cggactgcaa tcttcaagcc
     6901 aacagcaaac tgttcccgcc tccgagggag aaggcaaagc ggttaccgac caaaccgaat
     6961 tttccgcatc cgcaacaaac cctctcccca gccctctccc gcaggagagg gaacaaagtg
     7021 ccgccgccgt ttcagacggc ctgaaagcag ccgccgtttc aaccgaaagc agcctgtatc
     7081 tcgtcgcaag tgatctgcac gggcaaaccc gacagcaatc tgccccctcc cccgtggggg
     7141 agggttgggg agagggcaaa gcggttgccg ctcaaagtgc catcgccgtc atcaccgaat
     7201 ccgatcttta ccaatacgtc gcccgttccc gcgtccacaa ccgccgtaag aaacacgccg
     7261 ctgtttcaga cggcctgttg cgcgaccttg ccgaaatcaa tatcggcgat cccgtcgtgc
     7321 acgaagaaca cggcatcggg cggtatacgg gcttggtaac gatggattta ggcggcgaaa
     7381 ccaacgaaat gatgttgctc gaatacgcgg gcgaagcgca gctttatgtg cctgtttcgc
     7441 aactgcattt aatcagccgc tactccggtc aggcgcatga aagcgtcgcc ctgcacaagc
     7501 tcggcagcgg cgcgtggaac aaggcgaagc gcaaagccgc cgaaaaagcg cgcgataccg
     7561 ccgccgagtt gctcaacctc tacgcccaac gcgccgccca atcgggacac aagtttgaaa
     7621 tcaacgagtc ggactatcag gcgtttgccg acggcttcgg ctacgaggaa accgaagacc
     7681 aggccgccgc catcgccgcc gtgattaaag atttgacgca ggcgaagccg atggaccgac
     7741 ttgtgtgcgg cgatgtcggt ttcggcaaaa ccgaagtcgc cctgcgcgcc gcgtttgtgg
     7801 cggtgatggg cggcaaacag gtcgccgtac tcgccccgac cacgcttctg gtcgagcagc
     7861 acgcgcaaaa cttcgccgac cgtttcgccg atttccccgt aaaagtcgcc agcctttcgc
     7921 gtttcaacaa cagcaaagcc accaaagcca cgctcgaagg catggcggac ggtactgtcg
     7981 atatcgttat cggtacgcac aaattggtgc aggacgacat aaaattcaaa aacttaggtt
     8041 tattgattat cgatgaagaa caccgcttcg gcgtgcgaca gaaagagcag ctcaaacgcc
     8101 tgcgcgccaa tgtcgacatc ctaaccatga ccgccacgcc gattccgcgc accctcagca
     8161 tggcgctcga aggtctgcgc gacttctcgc tgattaccac tgcaccgagc cgccgcctcg
     8221 ccgtgaaaac ctttgtcaaa ccgttcagcg aaggcagcgt gcgcgaagcc gtgttgcgcg
     8281 aactcaaacg cggaggacag gtatttttcc tgcacaatga agtagatacg attgagaata
     8341 tgcgcgagcg gctggaaacc ctgctgcccg aagcccgtat cggcgtggcg cacggacaac
     8401 tgcgcgagcg cgagctggaa caagtcatgc gcgacttttt gcagcaaaga ttcaatgtgt
     8461 tgctctgttc caccatcatc gaaaccggca tcgacatccc caacgccaac accatcatca
     8521 tcaaccgcgc cgacaaattc gggctggccc aactgcacca gcttcgcgga cgcgtcggcc
     8581 gcagccacca ccaagcctac gcctacctgc tcacgcccga atacattacc aaagacgcag
     8641 aaaaacgcct cgacgccatc gcggcggcag acgaactcgg cgcaggtttc accctcgcca
     8701 tgcaggattt ggaaatccga ggcgcaggcg aaatcctcgg cgaaggacaa tccggcgaaa
     8761 tgatgcaggt cggcttcacg ctctacaccg aaatgctcaa acaggccgtg cgcgacctca
     8821 aaaaaggccg ccaacccgac ctcgacgcac cgttgggcat taccaccgaa atcaaactgc
     8881 acagcccggc attactgcct gaagactact gccccgacat ccacgagcgc ctcgtcctct
     8941 acaaacgcct cgccgtatgc gaaaccgtgc agcaaatcaa cgccatacac gaagaactcg
     9001 tcgaccgctt cggcctgccc gaacaacccg tcaaaaccct cattgaaagc caccacctgc
     9061 ggcttgcggc aaaagaactg ggtattgatg ccatcgatgc cacaagtgaa gccgtaaccg
     9121 ttaccttcgg caaacaccac tgtatcgacc cgacggggat tatcctgctg attcagacgg
     9181 ataaaaaata ccggctggca ggggcagaca aattgaggtt tgcagcggaa atggaaaaca
     9241 tcgaggtcag gatcaatacc gtcaaaaccg tactaaaaac cctgcaaggc aaacgcctgc
     9301 ccaaaggaaa ttgatgtaac gatgccgtct gaagaacacc gttcagacgg catacaatcc
     9361 cccaatcctc accaaaagga acaatcatgt cgatttttca cccctatttc caacaactgt
     9421 ccaccgaagg ttttgacgga gaagaagatg tcctctggga agacgagctg accgtcaaag
     9481 gcaatcttgt cgaggtattc ctgtaggcag aaaatgaaaa cagcctgcca accgaaacat
     9541 tggatgcatt tgccgccttc ctgtccgaat tggaaaaagc cgacgatgct gcacgcgccg
     9601 cactgacgga atatttgaaa cgggacagcc gctatatcga ctttcacacc gaagataccc
     9661 aaacaagccg tgatgcggca aaatttgtcc gcactatgca gttgatctat atcagcttat
     9721 gggcaaaaaa tccggctttt gccgttatgg attatatgcc cgccaatatg gaaagcgacg
     9781 aaatccttgc cgtcaaactg catttggacg gcagcatttt ctcaatcgat tgggagagtt
     9841 gagccggatt gccaatcggc aaaaatgaaa tgccgtctga aaacagcttc cctgcccgaa
     9901 gccttgccat tatgaatttg aaggaaactc cactataata cggcattcag attttcagac
     9961 ggcatcacgc ccgtcagacc gcacacaaac caaaaggaaa tacatgttcc gtactatact
    10021 tggcggaaaa atccaccgcg ccaccgtaac cgaagccgat ttaaactacg tcggcagcat
    10081 taccgtcgat caagacctgt tagacgcggc aggcatctgc cccaacgaaa aagtcgccat
    10141 cgtcaacaac aacaacggcg aacgttttga aacctatacc attgcaggga aacgcggcag
    10201 cggcgtgatt tgcctgaacg gtgctgcagc caggctggta cagaaaggcg acatcgtcat
    10261 cattatgtct tatatccaac tttccgaacc ggaaatcgcc gcacacgaac ccaaagtcgt
    10321 cttagtggac ggaaacaata aaatccgcga catcatctcc tacgagccgc cgcacaccgt
    10381 actgtaatcc cgcaaacgga catcgattat ggatattaaa atcaacgaca tcaccctcgg
    10441 caacaattcg cctttcgtcc tattcggcgg catcaacgtt ttagaagatt tggattccac
    10501 cctccaaacc tgtgcgcatt acgtcgaagt tacccgcaaa ctgggcatcc cctatatctt
    10561 taaagcctct ttcgacaagg caaaccgctc gtctatccat tcctatcgcg gcgtaggctt
    10621 ggaagaaggc ttaaagattt ttgaaaaagt caaagcagag ttcggcatcc ccgtcattac
    10681 cgacgtacac gaaccccatc aatgccaacc cgtcgccgaa gtgtgcgatg tcatccagct
    10741 tcccgccttt cttgcgcggc agaccgattt ggtggccgca atggcggaaa cgggcaatgt
    10801 tatcaacatc aaaaaacccc agttcctcag cccttcgcaa atgaaaaaca tcgtggaaaa
    10861 attccgcgaa gccggcaacg ggaagctgat tttatgcgaa cgcggcagca gcttcggcta
    10921 cgacaacctc gttgtcgata tgctcggttt cggcgtgatg aaacaaacct gcggcaacct
    10981 gccggttatt ttcgacgtta cccattccct gcaaacccgc gatgccggtt ctgccgcatc
    11041 cggcggtcgt cgcgcacagg ctttggattt ggcacttgca ggcatggcaa cccgccttgc
    11101 cggcctgttc ctcgaatcgc accccgatcc gaaactggca aaatgcgacg gccccagcgc
    11161 gctgccgcta caccttttag aaaatttttt aatccgcatc aaagcattgg acgatttaat
    11221 caaatcacaa ccgattttaa caatcgagta acacggtttc gccttatgat gcagactttc
    11281 cgaaaaatca gcctgtatgc cgcaaccttg tggctcggta tgcagattat ggcaggttat
    11341 atcgccgcac cggtgctgtt caaaatgctg cccaaaatgc aggcgggcga aattgccggc
    11401 gtattgttcg acatcctctc ttggagcggg cttgccgttt ggggcacggt actggctgcc
    11461 gcctttgccg ccctaacccg gcggcaaacc gccctgctgc tttttttatt gtccgccctt
    11521 gccgccaacc aatttttggt tacacccgtt atcgaggcac tgaaatacgg gcatgaaaat
    11581 tggctgttgt cggttgcagg cggatccttc ggaatgtggc acggtatttc cagcatgact
    11641 ttcatggcaa ccgccctact ttcagcagtt ttaagttggc ggctttccgg caaagaggcc
    11701 gtctgaagcc ctcccatttt ttacctccct tcacttcact tggagaacat tcatgagcgc
    11761 aatcgttgat attttcgccc gcgaaatttt ggactcgcgc ggcaacccca cagtcgagtg
    11821 tgatgtattg ctcgaatccg gcgtaatggg acgtgcggcc gtaccgagcg gcgcatccac
    11881 cggtcagaaa gaagctttgg aacttcgcga cggcgacaaa tcccgctatt ccggcaaagg
    11941 cgtattgaag gcggtcgaac acgtcaacaa ccaaatcgcc caagccctca tcggtatcga
    12001 tgccaacgag caatcttata tcgaccaaat catgatcgaa ttggacggta ctgaaaacaa
    12061 aggcaatttg ggtgcgaatg cgactttggc ggtctctatg gcggttgcac gcgccgctgc
    12121 cgaagactca ggcctgccgc tttaccgcta cttgggcggc gcaggtccga tgtccctgcc
    12181 cgtaccgatg atgaacgtca tcaacggcgg cgaacacgcc aacaacagcc tgaacatcca
    12241 agagtttatg attatgcccg tcggcgcaaa atctttccgc gaagcgttgc gctgcggtgc
    12301 ggaaattttc cacgccttga aaaaactgtg cgacagtaaa ggcttcccga ccacagtcgg
    12361 cgacgaaggc ggtttcgccc ccaacctgaa cagccacaaa gaagccctgc aactgatggt
    12421 cgaagcggcc gaagccgccg gctacaaggc gggcgaagac gtattattcg cattggactg
    12481 cgcgtccagc gagttctaca aagacggcaa ataccacttg gaagccgaag gccgctccta
    12541 caccaacgcg gaatttgccg aatacttgga aggcttggtt aacgaattcc cgattatttc
    12601 cattgaagac gggatggacg aaaacgactg ggaaggctgg aaactgctga ccgaaaaatt
    12661 gggcaaaaaa gttcaattgg tcggcgacga cttgttcgta accaatccga aaattcttgc
    12721 cgaaggcatc gaaaaaggcg tagcaaacgc attgctggtc aaagtcaacc aaatcggtac
    12781 tttaagcgaa accctgaaag ccgtcgatct ggcaaaatgc aaccgctacg ccagcgtgat
    12841 gagccaccgc tccggcgaaa ccgaagacag taccattgcc gacttggcag tcgccaccaa
    12901 ctgtatgcag attaaaaccg gttctttgag ccgttccgac cgcatggcga aatacaacca
    12961 actgctgcgt atcgaggaag aattggcgga agccgcctac taccccggca aagccgcatt
    13021 ctaccaactg ggcaaataaa taaaaaagtg gatgtatgtt tcaacacaca gggcaacaca
    13081 tgaagcgtcg ccctgtgtgt tgacctgatt tggaagaggt tacacctctc ccaaataaag
    13141 tctaatttta ccgccacgaa ggacggacat ccgagtggtg gggtttcaac cagtaaggaa
    13201 acgttgatga agtgggtaac tgtcgtttta tccttcgcac ttgtctgttg ccaatacagc
    13261 ctgtggttcg gcaaaggcag catcggacgc aacagcagtc tgagagaaca gattgccgtt
    13321 caagaagaaa aaaaccagac actcgcccta cgcaatcatt cccttgccgc cgaagtctat
    13381 gatttggaaa acggtcaaga agccatttcg gaaatcgccc gggtagaact gggttatatc
    13441 caagacggcg aaacctttta ccgactcatc aggcataacc ggtaataccg tcaaaaagcc
    13501 gtccgaacca atgttcggac ggcttttatt tcaacaaact gtcagacagc ccctcatcct
    13561 cccccgccaa accgcaatcc agcctgacat ccccctcgac gcaacagcag cacggcagta
    13621 tctcgtcccg ccccaaaaaa gccaaaggcg gctcccgata agtaacgctt ccctccaaaa
    13681 tctttacccg gcacgatccg caatatccgc ttcggcactg atattccacc atatgccccg
    13741 tccgttccaa gccttccaac agggtctcac cctccaagag ctcaaaaagg cccttattcg
    13801 taccaatgcg cgccatttcc gaccaatcaa aaataaaagg aacaataacc gatcctacct
    13861 gctgtttttc ccatcataca acacacaaat gccgtctgaa acatccggct tcagacggca
    13921 ttttttcaaa aaacatttac aactcaaaat cgcccaagtc atccgtattc acttcagaat
    13981 cgatttgacc aatcaaataa gaggatattt ccacttcctg cggcgcgacc tgtacgttgt
    14041 cggacgacag ccacgcatta atccacggaa tcgggttttg atttgcgcct tcaaatccgg
    14101 ccgacagccc caccgcctgc atacgcagat tggtaatata ttcgacgtat tgggataaga
    14161 tttctttgtt caaaccgatc atcgaaccgt ctttaaacaa atatgccgcc cattcttttt
    14221 cctgttccgc cgcttttttg aagagttgga aacattcgtc ctgcaactcg gcggcaattt
    14281 ccgccatttc cggatcatca acacccgaac gcatcagatt aagcatatgc tgcgtgccgg
    14341 tcaggtgcag ggcttcatcg cgggcaatca gtttgatgat tttggcgttg ccttccatca
    14401 actcacgctc agcaaaagca aacgagcagg caaatgaaac gtagaaacgg atggcttcca
    14461 acacattgac gcacatcagg cagagataga gttttttctt caacccgcgc aaagacacag
    14521 taacgggttt tccgccgaca ttgtgcaccc cttcgcccaa caggttgtaa tattgggtgt
    14581 attcgattaa atcatcataa tagcaggcaa tgtcttcggc gcgggcggta atgtattcgt
    14641 tttcgacaat atcatcaaac acgaccgacg gatcattcac aatattgcgg atgatgtggg
    14701 tatagctgcg cgagtggatg gtttcgctga agctccacgt ttcaacccaa gtttccagct
    14761 cgggaatcga aaccaaaggc agcaaggcaa cattcggact gcgcccttgg atggaatcga
    14821 gcagtgtttg atatttcaga ttgctgatga aaatatgttt ttcgtgttcg ggcaggttgg
    14881 cgtagtcgat acggtcgcgc gacacgtcga tttcttccgg ccgccaaaaa aacgacaatt
    14941 gtttttcaat cagtttttca aatacctcgt atttctgctg gtcataacgg gcgacattta
    15001 ccggctgacc gaaaaacatc ggctctttca gcgcgtcgtt tttggttttg ggaaaggtgc
    15061 tgtatgacat gactaggtgt tcgcaggaca taacgtctct ctttttaaaa attagtcgac
    15121 ttttctatta atttttaatt caccatataa aacatgttga attctatcaa taaagtgcca
    15181 tcctaaaata gcaaatacta aaagaacact aataaatata taaaatttat attcaactaa
    15241 gaatgaccga atgacatcca tacttttcct tttctggaaa ataacaactt agcctcatat
    15301 agtagtaggc actaatttaa gaaaatcctt gaagcctcct actatctaga agccttggat
    15361 atatttcaaa aattaacctg ttttaaatct tacaagcccc accagcgcag ccgtcatctt
    15421 gaatatcggt ctgcgtgtcg tccgcgccgt cgcgggtgtt gtggtagtac agggttttga
    15481 cgccgtattt gtaggcagtc agcaggtctt tgagcatttg tttcatagaa actttgttac
    15541 cttcgaattt gcccgggtcg taagcagtgt tggcggaaat cgcctgatcg acgaattttt
    15601 gcattacgcc gacaagtttc aggtagcctt cgttgcccgg aagctgccac agggtttcgt
    15661 aggcattttt caaggtttca aactccggca cgacttgttt caaaatgccg tctttcgatg
    15721 ctttgaccgt taccaatccg cgcggcggct cgatgccgtt ggttgcgttg gcgatttgcg
    15781 agctggtttc agacggcatg agcgcggtca gagtagagtt gcgcaggccg tatttgacga
    15841 tgtcggcacg caggctttcc cagtcgtaat gcaaaggctc gccgcagacg gcatccaaat
    15901 cttttttgta ggtgtcgatg ggcagtttgc cttgcgaata aacggtttgg ttaaagagcg
    15961 tgcacgctcc gtattctttg gcaaggtttg ccgatgcttt gagcaggtaa tactgtatgg
    16021 cttcaaaggt acggtgggtc agaccgagcg cggaaccgtc gctgtagcgg acaccgtttt
    16081 tcgccagata gtaggcgtag ttgatgacac cgatgccgag cgaacgccgg tccatagtag
    16141 aggtacgcgc ggcttctacc ggatatccct gataatctaa aagtgcatcg agtgcgcgca
    16201 cggtcaaatc ggcaagccct tccaattcgt ccaagctgtt taatgcaccc aagttgaagg
    16261 cggacagtgt acacagggct atttcgccgt cagggtcgtt gatgttgtcc agcggtttgg
    16321 tcggcagggc gatttccata cacaagttgg actgatgaac aggcgcaacg cgcggatcga
    16381 acgggctgtg cgtattgcag tgatcgacgt tttgaatgta gatgcgcccg gtcccggcac
    16441 gctcctgcat cagcgtggaa aacaggtcgg cagccggaat gatacgcttg cgtatgttcg
    16501 ggtcttgctc gtatttcgta tagagccgct caaattcgtc ttggtcggca aaaaacgctt
    16561 cgtacaaccc cgaaacctcg ttgggcgaaa acagcgtaat gttgccgccc ttaatcaggc
    16621 gggtgtacag caggcggttg atttgcacgc cataatcaag ctggcggata cggttgtctt
    16681 ccacgccccg gttgtttttc aacaccagca ggctttcggc ttcaatatgc cacaaggggt
    16741 agaacaaggt tgccgcgccg ccgcgcacgc caccttgcga acaagatttg accgccgcct
    16801 gaaacatctt aaagaaggga atgcagccgg tatgccgggc ttcaccgccc cggatttcgc
    16861 tgtccagccc gcggatacgt ccggcattga tgccgatgcc cgcgcgttgg gaaacgtatt
    16921 tcacaatcgc gctggtagtg gcattgatgg aatccaaact atcgtcgcat tcaatcagca
    16981 cacagcttga gaactggcgc gtaggcgtac gcacgccgct cataatcggc gtgggcaacg
    17041 atactttgaa tgtggaaacg gcatcgtaaa accgtttgac gtaacccaag cgcacctctt
    17101 tcgggtattt gctgaaaagg cacatcgcca ccaaaacata taaaaactgc ggcgtttcgt
    17161 aaatctggcg ggtaacgcgg ttctgtacca gatatttgcc ttcgagctgt ttgacggcgg
    17221 cataggaaaa ggacatatcg cgttcgtggt cgatataggc gttcagttcg tcaaattctt
    17281 cgcggctgta atcctcaagg atatgcctgt cgtattttcc ggcatcggtg agctttttaa
    17341 catggttgta aaggtgcggc ggctcgtatt cgccgtaggc tattttacga agatggaaaa
    17401 tcgccaaacg tgcggcaagg tattggtagt ccggggtatc ttccgaaatt aaatctgcag
    17461 cggctttgat gatggtttcg tggatgtcgt cggtgcggat gccgttgtag aactggatgt
    17521 gcgatttcaa ctcgacctgc gacacggaaa cattttccaa tccttccgcc gcccaagtaa
    17581 cgacacggtg aatcttatcc aaatcaatgg cttccaatct tccgtctcgt ttggttactt
    17641 ttaaatcagt cggtgtattc atcgcttcct cttccactct tgatattcaa gacacggtct
    17701 tttcaaataa attaaggcag acaatatagt ggatttttag cattttctcc agtcttgaca
    17761 acgattgcat ttttcagttt caggcaatgc gcgataaaac ccgcctgtcg tatttttcct
    17821 ataacatttg ttttatctga taattcttta ccgataaaaa acgggtaaat tttttgcctt
    17881 ttgaccggct ccggctacaa ggcgatgaaa taaggatttt ccgacgaaaa aggaaaactt
    17941 ccggttttcc gcccctgcga attgttaaat tttgcaaagt atgatttttg ccacgccgcc
    18001 gccgacaaat tccattttct taccgattgg aatttattat tgagattaat atgttatttg
    18061 aatttgcata tcaaacggca agttttgtcg gctgaatgag tctgaaactg ccgacagttt
    18121 gcccgttcca tccttccatc ctatactgga aggaattaca aactgaaagg atcgtcatgt
    18181 ccgtcaataa agatgcttgc catactgttg tctgcaatac tgggactggt atcaacaact
    18241 gccgctgccg gtacgtcaga acccgcccac cgacatacca aacatatcag caaggcaaac
    18301 aagcagatgc tgcaccccga atgcaggaaa tatttggaac gccgtgccgc gtggtaccga
    18361 tcgcaaggca acgtgcagga attgcgcgaa aacaaaaagg cgcgcaaagc attccgcacc
    18421 ctgccttatg cggaacagaa aatccaatgc cgggcggctt atgaggcttt cgatgatttc
    18481 gacggcggca ggttccgccg ttaatcccat ataaaatatg ctgtctgaac acaagttaag
    18541 atggcatttt ccatagatat agtggattaa atttagaccg gtacggcgtt gcctcgcctt
    18601 gccgtgctat ttgtactgtc tgcggcttcg ccgccttgtc ctgatttaaa tttaatccac
    18661 tatacaacaa actatcctta cggcatttct tttgaggtac tcccgttacc gcagccttaa
    18721 ggcatccgca aaagcgaatc ataccgccac attccgacaa ccgctttcag acggcaacat
    18781 ccgcatcgcc tgcgacaacg gcacggatgc tttcttcgat tttatctttt aaagcataac
    18841 ggtcttcgct ttccgccgcg tccgccacgc aaacgaaatc gactttgata gtcagttttt
    18901 tcatagacac gattcgccac aggcaagtcg gcaaaccgac atcggcatac gacggacggg
    18961 ccgtcctttt tcccgtttcg tcataataac gcagtgcgac cgccaaaacc tttgcccccg
    19021 catcgatggc ggattggaac agcgcagcct tgaacggcag gagccccagt ccggaggaag
    19081 ttcgcgcctc agggaaaaaa ctgacgtttt gaccgcgttg caaggtttcg cagacggcgc
    19141 ggttaatcgg ttcgatgtcg cgccgcgaat tgcggttgat gaacaccgtt cccgcgttct
    19201 gccccatctt gcccaatacc ggccagcttt tgatttcctg cttggcgata aagctactcg
    19261 gataaaccgc gctcatcgcg aaaatatcca gccaggacac gtggttggcg gcaaccaaga
    19321 caccgttcgg atgttcgggt gcgggtctgc ccacctccaa tccgatatcc aaagccgcca
    19381 aagcgccctt gcccaacgct ataactgccc gattgcgcga cttggggcag ccgccgtcaa
    19441 taccgcgcag gtttttgccg gttttgaaca gccagaccgt caagcggcac aagcggcgca
    19501 gacgtgtaaa aaatgaagct ttatttgaag acatgggcaa ttctttcccg ataatcggtg
    19561 tttgttgttg aaacgttcgg acggcatacc gataaaatgc cgtcagaaac cgttttccgc
    19621 cctatttctg acagttcgtg caataaaacg tgccgcgctg ccccaaagtt tctttcacaa
    19681 ccaaaccgcc gcaccggagg cacggctgat tgtgccgccc gtacaccgta tattcctgtt
    19741 gaaagtagcc gcttttgccg tcgctgtcca caaaatccct cagcgtactg ccgcccgttt
    19801 caatggcgcg ctgcaacacc gctttgacgg tttcaaccaa aaccgcgcac tctttctttt
    19861 ttaggcggtt ggcaggacgg tggggcgaaa tgcccgccct gaacagactc tcgttggcat
    19921 aaatattgcc cacgccgacc acgacggtat tgtccatcag agcgagtttg accgcgcgtt
    19981 tctgcgcctt cagccccgca tacagataat ccgtgcaaaa tgcctccgac aaaggctccg
    20041 gccccagttt ttccaacagc ggatgacgtt cttcgattcc ctcataccaa agtatcgcgc
    20101 cgaactttct cggatcgcgg taacgcatga ccgtgccgtc tgaaaacaca atatcgacgt
    20161 gatcgtgtct gtccggcctg ccgatacgtc cgtccgacgg cgtaaagatc cgcaagctgc
    20221 ccgacatccc caagtgaatc agcaaaatcc ccgtttggaa acggacaatc agatatttgg
    20281 cacgcctgcc gcaggacaaa acctgccggc cggacaaaat ctcccccaaa tcgggattga
    20341 tctgccagcg cagcttcagt tggcgcaata tcacggcttc caccgttttt ccttcaatat
    20401 gcggcgcgat gccgcgcaac gtcgtttcca cttccggcaa ttccggcata acccctcccg
    20461 acatttcttc tgacagatgc cgtctgaaag acggccgtcc cttaatccgc aacccttgcc
    20521 gcacccgccg caagggcttt gccgccccaa tacccgtccc acgcgggcag gggcgttacg
    20581 cccgccgccc ttttgaccaa aaccaaaccg tacactgcgg cacattgcgg ccaccaacgg
    20641 tcgcccgcct tttccataaa ccgccaaaag cgtatttgcc cgagcgacga aacgggaggc
    20701 agatacacca tgaatttccc aaattcaata tcgaaaccgg catcggcaag ctgccttttc
    20761 aactcgggca gcggcagaca aaaccgtttt tccggcagac ggacaccgtc aaaccaacgg
    20821 cagaatcccc aaagcgaata cggattgaaa cccgtcagca tcaaacgtcc ggacggtttc
    20881 aatatccggt gcgcttccga caggatttgc gaaggtacgc cgcattccag cgtatgcggc
    20941 aaaagcagca tatccgcaaa aacatccgcc aaagccatat tctccgccga catcgacata
    21001 tctcgcggca cacagacaat gtcttcagac aggctcagcc actgaccgcc cacctgaacc
    21061 gcacacattc ccgaaaaacg gtatgacgcc acataccgat taaagaagtc ctgttccaat
    21121 tttgcaacat accgccccat cgccgtatct tcaaaccatg catccatatt gcgtccgttt
    21181 caaacagatt gcctgccgat attttattcc aaacaggatt tctgtcaaaa aacacatcgg
    21241 ccgcccattt ccgaatccgc ataaagttcc tgtaaaactt gacgcttttt cagtcaaaca
    21301 gtaccatcgg acgataaaat atgtttattc cgccgagtat aaatcatgtc caaactcaaa
    21361 accatcgccc tgaccgcatc aggtctgtcc gtttgtccgg gtttcctata tgcccaaaac
    21421 acctcatcac accaagtcgg tttagcgatt atgcggttaa actcttcaat actcgacctg
    21481 ccaccgacaa aacaatattt ccaatccggc agcctgtggg acgagctgcg ccaaggcttc
    21541 cggatgggcg aagtcaatcc cgaactggta cgccgccacg aaagcaaatt catcgcaagc
    21601 cgcagctatt tcgacagggt cgtcaaccgg agccgaccct atatgtacca tatcgccaac
    21661 gaagtcaaaa aacgcaatat gcccgccgaa gccgccctgc ttcccttcat cgaaagcgcg
    21721 ttcgtcacca aagccaaatc acacgtcggc gcatcgggcc tgtggcagtt catgcccgct
    21781 accggcaggc attacggctt ggaaaaaaca ccggtttacg acggcaggca cgacgtttac
    21841 gcagccaccg atgccgcact caactatctg caatatctct atggactgtt cggcgactgg
    21901 ccgctcgcct ttgccgccta caactggggt gaaggcaacg tcggacgcgc cgtcaaccgc
    21961 gcccgcgacc aagggctcga accgacctac gaaaacctgc gtatgcccaa cgaaacgcgc
    22021 aactatgtcc ccaagctgct cgccgtgcgc aacattattg ccacccccca atctttcggc
    22081 atgaatatca gcgacataga caacaaaccc tattttcagg cagtcgaacc gggccgtccg
    22141 ctcgacaacg aagccatcgc ccggcttgcc ggcatcacgc aaagcgagct gctcgccctg
    22201 aatcctgcat tcaacgtccc cgcgttcatc cccaaaaaca aacgcaaact gctgcttcct
    22261 gtcgcgtccg tccaaacctt ccaaagcaac tacctcaacg ccgcacccga cagcctgttt
    22321 tcatgggaag tctatacgcc tgccgccaaa accagcctgt ccgacatctc gacggcaacc
    22381 ggcatgagca ttgccgacat caaacgcctc aacaacctga acggcaacct tgtcaacgca
    22441 ggacgcagca tccttgtcgc caagaacggc aagacccttc atacggcatc ggaatccgtc
    22501 gtttccatcg acatcgacaa tacgcccgac acctaccgtt ccaatatgcc ggcaggcacg
    22561 gtgaacgtca gcattgcccg aatccaaccc gccgccgcac agacagcgga cattaccgtc
    22621 gcacctttgc cgcaagaaac cgtccgtacg gaacccgatc cccttgtccg cattgccgaa
    22681 cccgcccttg cgacagccgc agcgcaacct caaaccgaaa aacagactgc catgccgtct
    22741 gaaacccaaa ccgcaacact cgcgcaggtc atccccccaa acgacatgca ggcggcagac
    22801 gaactcatgc agcttgttgc ccgaaacaac ctgcgccggc aggctgaaga aaccatctcc
    22861 gccgtcatcg gcacgcctga cacagttgcc gaacacaaca tttcctcatc tccgcaacat
    22921 accgttgccg ccgacggcaa acgccgggca cgtttggaaa cgcgcgtagc caaagctgcc
    22981 gacggcgaag ccgaaacctc cccgctccat gccagcatcc accgcgttgt agaaggcgac
    23041 accctgttca acattgccaa acgctacaac gtcagcgtag ccgacctgat tgtcgccaac
    23101 aacatcaaag gcaacaccat ccaaaaagga caggtactcc gcctggcgca ggcagcccct
    23161 gcccaaaccc gtattgaaaa agtatcctac accgcgcgca aaggcgacac cttcaaaagt
    23221 atcgccgcgc gcttcaatat ccatatcgac gacatccgcc ggctcaatcc caacctgaac
    23281 accatcaatc cgggacagag ggtcaaactg attggaagct gattcggata cggcacatga
    23341 caggactttc tcagtcctgt ctttttcatc ccacatttcc cataccatca tgaaactcat
    23401 caaatacctg caatatcaag gcataggaag ccgcaagcag tgccaatggc tgattagggg
    23461 cggctacgtc tccatcaacg gaacctgcat ggacgacacc gatgcagaca tcgattcctc
    23521 atacgtcgaa acgttggata ttgacgggga agcagtaacc gtcgttcccg aaccctattt
    23581 ctacatcctg ctcaacaagc ctgaagatta cgaaacttcg cacaaaccga aacactaccg
    23641 cagcgtattc agtctgttcc ccgacaatat gcggaacatc gatatgcagg cggtcggcag
    23701 gctggatgcg gatacgaccg gcgtattgct gattaccaac gacggcaaac tgaaccacaa
    23761 cctgacttcg ccgagcagaa aaattcccaa gctatacgaa gtaacgctca aacaccctac
    23821 aggagaaacg ctctgcgaaa ctttgaaaaa cggcgtgccg ctccacgacg aaaacgaaac
    23881 cgtttgtgcc gccgatgccg ttttggaaaa tccgaccacc ctgctgctga ccattaccga
    23941 aggaaaatac caccaagtca aacgcatggt tgctgccgcc ggcaaccgcg cgcaacacct
    24001 tcaccgccgg cgattcgcac atttggaaac agaaaacctc aaacccgggg aatggaaatt
    24061 tatcgaatgt ccgaaattct gaaacaaaat tccataattt ccctatattc aaccatatgc
    24121 ttattaaata agcagtttta caaaaattaa ctcaaaaaaa cagttagttt acaatataat
    24181 ccgcttgtct aagtaacgcc tgttcaagca ggattatgag caagacgttt tccccgtaat
    24241 gtgttttgcc atctatactt ctccccttgt atagatggtt ttctatttca aattccctct
    24301 ctttcatcat cattgaaatg ctatcattaa tatgattaac aaaatttcac ttccgtacac
    24361 cgatgtagaa aaataacaga ttaatctgtt cataaactcc gattcttcct catcggttta
    24421 tactcatcag aaatattcac tgaaatatat gcattttaca tggaaaatca ctaggcagtt
    24481 gaaaaacatt cagtcaattt ctgttaagaa tcacgtgtaa aatttccgct ttatctcaat
    24541 cggacacggt ttcaatatgt cttccccttc aaataccaac cgtcaaacct ggtccagccg
    24601 tttgacttat atccttaccg ttgccggcgc gactgtcggt ttcggcgcga cgtggcgttt
    24661 cccgtatttg gtcggtgaaa acggcggcgg cgcgtatgtg tttttattct gtatcgcgat
    24721 gctggttatc ggcatcccga tgattttggt ggaaaacgtc atcggacggc gcaaaggcgt
    24781 gaacgcgctg gatgcgttcg gcggcccgat gaacggcaaa cctgttgcca aaatttggaa
    24841 actggtcggc cggatggggc tgctcggcgc gttcggcatc atggcttatt atatggtact
    24901 cggtggctgg gtaatcagct atatcgttaa tattattgga ggaaatttga atatttccag
    24961 ccccgtcgac ggtgtggtta caaaaggctt ctttaccgaa cacattgaaa acagcccttg
    25021 ggaaattgcg ttttatacgc tgctttttgt cgccgtgaac caatggattt tggtcaaagg
    25081 cgttatcggc ggcattgaaa aaacggcgaa atacctgatg cctctgctgt ttttgttcct
    25141 aatcgcgatg gtcgtccgca acgttaccct tccgggcgca atggaagggg ttgctttcta
    25201 tctgaaacct gatttcagca agattaccgc cgaactgttc gtcttcgttt tgggacaggt
    25261 attttttgcc ctgagcctgg gtttcggcgt gatgattacc ttatccagct atctggataa
    25321 aaacgaaaat ctggttcaga cggcagttat cacggcaatt accaatacca tcatcgctgt
    25381 acttgcgggc tttatgattt tcccgtcgct cttcagcttc ggcgtcgcgc ccaattccgg
    25441 cccgactttg gtgttccaaa gtttaccgat tgtgttctca catatgtggg caggacctgt
    25501 gttcgccgtc attttcttct ctctgctcct gattgccgcg ttgacaactt cgctgacgat
    25561 ttatgaagtg ttgattacga ccattcagga aaaaaccaaa atccgccgta ccgccgcaat
    25621 cacgattgta ttgtccgtca tcttcgtttt cggcaacatc ccgtctattt tgagctatgg
    25681 cccgtggaaa gacgtttccg tgttcggcaa aaatattttc gatgccttcg actacatcag
    25741 cggcaacatc ttgtttatgc tgaccgcgct cggttccgca ttgtttgtcg gttttgtgat
    25801 gaaggacgaa gcgaaggaag aattgcttta taaaggcaac catacgacgg tcaatatttg
    25861 gtttgcctat gtgaaatacc ttgtgccgct ggtgattctg ctgattttcg tcagcaacct
    25921 gttctaatcc gcagcaatca atgccgtctg aaggtcatac ctctttcaga cggcattgca
    25981 ttgatgcccg ccacgccgga atccgaaccg gcttgccggc ttctgtcgga cgctttccgg
    26041 gcaggcggcg ttccgtctgc ttagacaatc gtcctttaaa acaggtagaa tccgccccaa
    26101 cgggaaacac acccttcaga cggcaaaacc cataccccaa accatcagga atccccctta
    26161 tgaacagaca aaaagtcatc gccatcgacg gtccgggcgc atcgggcaaa ggcacggtcg
    26221 ccgcccgcgt tgccgccgca ttgggatacg attatctcga taccggcgcg ctctaccgcc
    26281 tgaccgccct atatgcacaa aaacaaggcg tggaatggca cgatgaagaa aacgtttccg
    26341 cacttgccaa aaaactgccc gccgtatttt caggcaaccg catcctactt gatggcgaag
    26401 acgtttcaga cggcatccgg acagaagcca tcggtatggg cgcatccgca gttgcgcaat
    26461 ggcccaaagt ccgcgccgca ctgctgcaac gccaacgcga ttttctgacc gaaaaaggac
    26521 tggttgccga cggacgggac accggatcgg tcgtcttccc ccaagccgaa ctcaaaattt
    26581 ttctgacagc cgaatctaaa atccgtgccg aacgccgcgc caaacaaatc ggcattccct
    26641 gcgaaggttt cacattcgag cgcatcctgt ccgacatcga gaccagagac gaagcagacc
    26701 gaaaccgcaa ggttgccccc ctgaaacaac agtccgatgc cctgcttttg gatacgagcc
    26761 gcctaactat agaagaaact gtaaaaaaag tgcttgattg gtatcgtaaa gtttaaattt
    26821 tcaggtataa tcgcccgcat tgcgcttcag acagcatgaa tttccatgtg ccgtctgaaa
    26881 ccttatttac ccgcctgccg tgccaaggac tgcagacacc caccaaccca acccgcaccc
    26941 cttggcggtg taccgaaaag agttatatat gtctatggaa aattttgctc agctgttgga
    27001 agaaagcttt accctgcaag aaatgaaccc gggtgaggtg attaccgctg aagtagtggc
    27061 aatcgaccaa aacttcgtta ccgtaaacgc aggtctgaaa tcagaatccc tgatcgatgt
    27121 agctgaattc aaaaacgctc aaggcgaaat tgaagttaaa gtcggcgact tcgttaccgt
    27181 taccatcgaa tccgtcgaaa acggcttcgg cgaaaccaaa ctgtcccgcg aaaaagccaa
    27241 acgcgcagcc gattggatcg ctttggaaga agccatggaa aacggcaaca tcctgtccgg
    27301 catcatcaac ggtaaagtca aaggcggcct gaccgttatg atcagcagca tccgcgcatt
    27361 cctgccgggt tctttggtcg acgtacgtcc cgttaaagac acttcccatt ttgaaggcaa
    27421 agagatcgaa ttcaaagtga tcaaactgga caaaaaacgc aacaacgtcg ttgtttcccg
    27481 ccgcgccgtt ttggaagcca ctttgggtga agaacgcaaa gccctgctgg aaaacctgca
    27541 agaaggctcc gtcatcaaag gcatcgtcaa aaatatcacc gactacggcg cattcgttga
    27601 cctgggcggc atcgacggcc tgctgcacat caccgatttg gcatggcgtc gcgtgaaaca
    27661 cccgagcgaa gtcttggaag tcggtcagga agttgaagcc aaagtattga aattcgacca
    27721 agaaaaacaa cgtgtttcct tgggtatgaa acaactgggc gaagatcctt ggagcggtct
    27781 gacccgccgt tatccgcaag ccacccgcct gttcggcaaa gtatccaacc tgaccgacta
    27841 cggcgcattc gtcgaaatcg aacaaggcat cgaaggtttg gtacacgtct ccgaaatgga
    27901 ctggaccaac aaaaacgtac acccgagcaa agtcgtacaa ctgggtgacg aagtcgaagt
    27961 catgattttg gaaatcgacg aaggccgccg ccgtatctct ttgggtatga aacaatgcca
    28021 agccaatcct tgggaagaat ttgccgccaa ccacaacaaa ggagacaaaa tctccggtgc
    28081 ggttaaatcc attaccgatt tcggcgtatt cgtcggcctg cccggcggca tcgacggtct
    28141 ggttcacctg tccgacctgt cttggaccga atccggcgaa gaagccgtac gcaaatacaa
    28201 aaaaggagaa gaagtcgaag ccgtcgtatt ggcaatcgat gtggaaaaag aacgcatctc
    28261 cttgggtatc aaacaactgg aaggcgatcc tttcggcaac ttcatcagcg tgaacgacaa
    28321 aggttctttg gttaaaggtt ccgtgaaatc tgttgatgcc aaaggcgctg ttatcgccct
    28381 gtctgacgaa gtagaaggct acctgcctgc ttccgaattt gcagccgacc gcgttgaaga
    28441 cttgaccacc aaactgaaag aaggcgacga agttgaagcc gtcatcgtta ccgttgaccg
    28501 caaaaaccgc agcatcaaac tttccgttaa agccaaagat gccaaagatg ccaaagaaag
    28561 ccgcgaagca ctgaactccg tcaatgccgc cgccaatgcg aatgccggta ccaccagctt
    28621 gggcgacctg ctgaaagcca aactctccgg cgaacaagaa taaggttgca gacatgacaa
    28681 agtctgagtt aatggttcgc ttggcagaag tatttgccgc caaaaacggc acgcatcttc
    28741 tggcaaaaga cgtagagtac agcgtaaaag tcttggttga caccatgacc cgatcgcttg
    28801 cccgaggtca acgcatcgaa atccgcggtt tcggcagctt cgatttgaac catcgtcctg
    28861 cccgcatcgg tcgcaatccc aaaaccggcg agcgcgtgga agtacctgaa aaacatgtac
    28921 cccacttcaa gcctggtaaa gaattgcgcg agcgggtcga cttggcttta aaagaaaatg
    28981 ccaattaaac cttagcatca aaacgccgct ggtacgcggc gttttttctt tggtttaact
    29041 tcatccgctg cttcaatacc ttaagccaag caagcaacgg attagagcgt ggatttttta
    29101 ccattgctta caatccgctt tttaaacaac aaattgttga tttctattac gaacaggaca
    29161 aaatcctgct tattgcacta aaactaagcc gtttcaggaa tttgcggcaa atttacagct
    29221 tttaccgagc ctaatgcttt cgctttttgg taaaacgcca atttgtattc aagcaaatct
    29281 aaataacgtt ttaattcggc aatttgacac ttcacatttt ctatttgatt ttcaaacaag
    29341 gaaaggcgtt cttcaatggt atcgtcgcca atgacggtac attccgcaaa gcgtttgatg
    29401 tcttttaagc tcattcccgt atttttcaag cattgcaata agtacaacca ttgcaaatcg
    29461 ttatcggtaa aacaacggtt accgtattca tcacgtccga tattaggcaa caagccttct
    29521 ttgtcgtaaa agcgtaaagt gtgggcggag atgcctattt tttcggcagc tttggcagtg
    29581 gtataagtca ttttccctcc ctctaaacaa aaaacggtaa aaaaacactt gccttagagt
    29641 gaactctaaa atgtaaactg ttgctatgtt gctcaggcaa ggctattttg ttaatgaatt
    29701 aagaggaaag acaatggaaa tgaaacaaac cgattcaacc atcaaatctc gtgcggcggt
    29761 ggcattcgcc cctaaccaac ccttacaaat tgtggaaatc gacgtggaaa tgccgcgtaa
    29821 agacgaggtg ttaattcgta acacccacac tggcgtgtgc catactgatg catttacctt
    29881 atcaggaagc gatcctgaag gcgtattccc tgtggtgctt ggacacgaag gtgcgggtgt
    29941 ggtcgttgct gtgggcgagg gtgtgtcaag cgtaaaaccg ggtaatcacg tcattccgct
    30001 ttacaccgca gaatgtggcg aatgtgagtt ttgcctctcg ggtaaaacca atttatgcgt
    30061 ctcagtgcgt gatacacaag gtaaaggctt aatgccagac ggcacgacgc gtttttctta
    30121 tcaaggtcag ccgatttatc actatatggg ctgttcgact ttcagtgaat attctgttgt
    30181 tgccgaagtt tcactggcga aaatcaaccc gaaagccaac cacgaacaag tatgtttgct
    30241 cggctgcggc gttaccactg gtattggtgc ggtacataac acggcaaaag tacaagaagg
    30301 cgattctgtt gccgtgtttg gcttgggagc gattggtttg gctgtggtgc aaggtgcgcg
    30361 tcaagccaaa gccggtcgca ttatcgccat tgataccaat cctgcaaaat tcgagttggc
    30421 aaaacagtgt tggtgcaact gactgtttga gcccgaaaga ttacgataaa ccaatcaaag
    30481 atgtgttgtt agacatcaac aaatggggca ttgaccacac ctttgaatgt atcggcaatg
    30541 taaacgtaat gcgtcaggca ttagaaagtg cacaccgagg ttggggacaa tccatcatca
    30601 tcggcgtagc ggatgcagga caagaaattt caacgcgtcc gttccaatcg gtaacaggcc
    30661 gtgtttggaa aggctcggca tttggtggtg ttaaaggccg ctccgagctt ccgaaaatgg
    30721 tggaggattc aatgaaaggc gatatccaat tagaaccatt tgtgacccat gcaatgaccc
    30781 ttgatcaaat caatgaagcc tttgagttaa tgcacgaagg taaatcgatc cgcgctgtta
    30841 ttcattacta aggtccacga tgaaactgat tgaacaacat caaatttttg gtggttcgca
    30901 acaagtttgg gcgcatcatg cccaaacgct tcaatgcgaa atgaaatttg ccgtctattt
    30961 gccggataat ccggaaaatc aaccgcttgg tgtgatttat tggctttccg gcttgacgtg
    31021 tacggaacaa aatttcatta ccaaatcgag cttccggcgt tatgcggcag aacatcaagt
    31081 ggttgtggtc gcccccgata ccggccctcg cggagagcaa gtgccgaacg gtgccgctta
    31141 tgatttagga cagggggcag gcttttattt gaatgcgacc gaacagcctt gggcggcgaa
    31201 ttatcaaatg tatgattaca ttttgaacga gttgccccgt ctgattgagg agcattttcc
    31261 taccaacggc aaacgttcca ttatggggca ttcgatggac ggacacggcg cattggtatt
    31321 ggcactacgg aatcgggaac attatcaaag tgtttctgcc ttttcgccta ttttatcgcc
    31381 aagcctcgta ccgtggggag aaaaagcctt tactgcttat ttagggaaag accgtgaaaa
    31441 atggcagcaa tatgatgcta actcactcat tcaacaaggc tataaagtgc aaggtatgcg
    31501 tatcgatcag ggcttggaag atgagttttt gccgacacaa ttgcgtacca aagattttat
    31561 cgaaacctgt cgtgcggcaa atcagccaat cgatgtgcgt ttccataaag gatacgatca
    31621 cagctattac ttcatcgcca gttttattgg tgagcatatc gcttatcacg ccgcattttt
    31681 gaagtaagtc aaagagcggt cagttttcaa agcagtttgg aatagccggc acgagggcgg
    31741 taagaagtgc cggcataaac gtatgccgcc tgaaccggaa ggagccggcc ctacggatta
    31801 ccaaaccctg cccttgattt gattagtatg atttttaagc ttaaaccctg attgatcaaa
    31861 cattaaaatc gcccgtattt cctacttcac tttcgggctt cgtattattc gttgccgaat
    31921 ttgcataagc aacctgttcc tgttgtgttt tcgccccctc caagctgacg gtggcgtaac
    31981 agcttcgcga ttcaccatct gtttcatttg tcctccttca atattaaaac gcagcctgaa
    32041 ataaaaggca ttttggctca tatctgcacc atattaaaac gccgcctttg cttatacccc
    32101 ctttgtgcgc gtcattattc ttttccacgg gaaacgccaa gtttgaagga aatcatttat
    32161 aataccagcg gtaagcattt tctttcttag ccgcaagaag tataacaagg ttaaatatga
    32221 ataatagaga ccaacttttt aaagccccgc cgtttgaaaa ccacagcccg ctgacctggt
    32281 atcaggctgc ctcacaactg cccaacttca tccgcgacga cgcacaggcg gccgccatcg
    32341 aacaccttga ccggctttgg accgaattga tgatgttcaa acgcaaaaga aaccgttttt
    32401 taggcaggag tttgcgttcc ccgcaagttc ccaaagggct ttatttctat ggcggagtcg
    32461 gacgcggcaa gagctttctg atggacgctt ttttcggctg cctcccttac cgccgcaaac
    32521 gccgcgtcca ctttcatgcc tttatggcgg aaatccaccg gcggctgaaa gccctgaaaa
    32581 gcgaaagcaa cccgttgaaa tccgttgccg ccgagattac caaagaaacc cgagtattgt
    32641 gttttgacga atttcatgtc agcgatattg cggatgcaat gattttaggc cgtctgctgg
    32701 aaaacctgct taacgagggc gttgttttgg tggcgacttc aaactacgcg ccttccgaac
    32761 tctacccgca aggtcaaaac cggagcggtt ttcttcccac aatcgcgctc atcgagtcca
    32821 gtctgaccgt cttaaacgtt gacggagggg aagactaccg gctgcgtacc ctccgccccg
    32881 ccgagatttt ctttacgcct gccaatgaag aaaatgaggc aaaactggca aaactgttca
    32941 aagaaatgac aggcattacc gatttgaacc ccggcatcag caccatccac ggccgggaaa
    33001 ttccccacaa agccgagtcc ggtcgtacca tatggtttga tttccgcgca ctgtgcttca
    33061 gtccccgctc acagtccgac tatctgtatt tggccgaaca ttatgaaatg gtttttattt
    33121 caggtttgga acaactctca ccgcaagaaa aggcggaggc gcgacggctg acttggctga
    33181 ttgacgtact ctacgatttc cgggtcaaac tgtgtgccac cggcgcggta gatgtcaacc
    33241 atatctacac ggaaggcgat tttgccgaag aatttacccg caccgccagc cggatggtcg
    33301 aaatgcagtc cgaagtttat ttggaacagc cgcacctgac cttatctccc aaggcttcag
    33361 gcggataagt tatttttttg gtagaatacc gatttgattc ttcttaagta aaaataagga
    33421 tatagcatgg cgattgaacg taccatatcc atcatcaaac ccgatgccgt cggcaaaaac
    33481 gttatcggca aaatatacag ccgctttgag gagaacggtc tgaaaatcgt tgccgccaaa
    33541 atgaagcagc ttaccctcaa agaagcccaa gaattttatg cggttcataa agaccgcccc
    33601 ttctatgccg gattggttga atttatgacc ggcggtccgg ttatgattca agtactggaa
    33661 ggcgaaaacg ccgttctgaa aaaccgcgaa ctgatggggg cgaccaatcc caccgaagcc
    33721 gcagaaggca cgatacgcgc ggactttgcc acttcggtca gcattaacgc cgtacacggt
    33781 tccgacagtg tggaaaatgc cgctttggaa attgcctact ttttcagcca aaccgaaatc
    33841 tgcccccgtt gatacaatac accgcccaat tcctattcag acggcataaa atatatccat
    33901 gccgtctgaa aactctgttg caaaagactt caaatcaaac ttgcctgccc tgcaattttt
    33961 tatttgaagc cttaatttaa gaaaaacaca aatacatgaa aaccaatctg ctcaactacg
    34021 accttcaagg gctaacccga cattttgccg atatgggtga aaaaccattc cgcgccaaac
    34081 aggttatgcg ttggatgcac caatccggcg cgcaaaattt tgacgaaatg accgatttgg
    34141 caaaatcgtt gcgccataaa ctgaacgaac aggcaagcat cgaaattccc aagctgatga
    34201 tgtcccaaga atcttcagac ggcactcgaa aatggctttt ggatgtcggt acggggaacg
    34261 gcgtggaaac cgtcttcatc cccgaatcgg atcgcggcac gctctgcatt tcctcgcaag
    34321 tcggctgcgc tttggaatgt acattttgtt cgaccggccg gcagggcttc aaccgcaatt
    34381 taaccgctgc cgaaatcatc ggacagttgt ggtgggcaaa caaagcgatg ggcgttacac
    34441 cgaaaaacga gcgtgtgatt tccaacgtcg tcatgatggg catgggcgag ccgatggcga
    34501 acttcgacaa tgtcgttacc gccttgagca tcatgctgga cgaccacggc tacggtttga
    34561 gccgccgccg cgtaaccgtt tccacttcgg gtatggttcc ccaaatggac aggttgcgcg
    34621 atgtcatgcc ggtggccttg gcggtttccc tccacgcttc caatgacgaa gtccgcaacc
    34681 aaatcgtacc gttgaacaaa aaatatccct tgaaagaatt gatggccgca tgccaacgct
    34741 atctggtcaa agcgcccagg gatttcatca ctttcgagta tgtgatgctg gacggtgtca
    34801 acgataaggc acaacatgca tacgaactta ttgaactggt caaagatgtt ccctgcaaat
    34861 tcaatttaat cccgtttaat cctttcccta attcgggata tgaacgttca agtaacgaaa
    34921 acatccgtat tttcagagat atcctgcaac aagccgaatt tgtcgttacc gttcgaaaaa
    34981 ctcgtggcga cgatatcgat gccgcctgcg gacagttggc aggtcaggtt caggataaaa
    35041 cgcgccgcca acaaaaatgg cagcagattt taatcggaca acaggggtaa ttatgccttt
    35101 taagccatcc aaacgaatct ctttattact tgttcttgcc ttgggcgcgt gcagcacttc
    35161 ctaccgcccc tcgcgggcag aaaaagccaa tcaggtttcc aatatcaaaa cccagttggc
    35221 gatggaatat atgcgcggtc aggactaccg tcaggcaacg gcaagtattg aagatgcctt
    35281 gaaatcgaac cctaaaaacg agcttgcctg gctggtccgt gccgaaatct atcaatacct
    35341 gaaagttaac gacaaggcgc aggaaagttt ccggcaagcc ctctccatca aacccgacag
    35401 tgccgaaatc aacaacaact acggctggtt cctgtgcggc aggctcaacc gccctgccga
    35461 atctatggca tatttcgaca aagccctggc cgaccccacc tacccgaccc cttatattgc
    35521 caacctgaat aaaggtatat gcagcgcaaa acaggggcaa ttcggattgg cggaagccta
    35581 tttgaaacgt tccctcgccg cccagccgca gttcccaccc gcatttaaag aactggcgcg
    35641 caccaaaatg ctggccgggc agttgggcga tgccgattac tactttaaaa aataccaaag
    35701 cagggtagaa gtccttcagg ccgatgattt gctgctaggc tggaaaattg ccaaagccct
    35761 cggcaacgtg caggcggcat acgaatatga agcacaattg caggcaaatt tcccctactc
    35821 ggaagaattg caaaccgtcc tcaccggtca ataaacagat tcaaaccata tgaacacact
    35881 ccaacgccgc aagacgcatc aagtcctcat cgatcatatc accgtcggtt cagaagcacc
    35941 cgtcgttatc caatctatga ccaacaccga cactgccgat gcaaaagcca ccgcattgca
    36001 gattaaggaa ttgagcgatg ccggctcgga aatggtgcgg attaccgtca acagccccga
    36061 agccgcgtcc aaagttgccg aaatccgccg ccgcttggac gatatgggct atgccacacc
    36121 actgattggc gatttccatt ttaacggcga acgcctgttg gcggaatttc ccgaatgcgg
    36181 caaagcattg tccaaatacc gcatcaatcc cggcaatgtc ggcaaaggcg taaaaggcga
    36241 tgaaaaattt gcctttatga ttcggactgc tgctgaaaac gataaagccg tccgtatcgg
    36301 cgtaaactgg ggttctttgg atcagagcct cgccaaacgt atgatggatg ccaacctcgt
    36361 ttcttccgcg ccgaaaccgc ccgaagaagt gatgaaggaa gcactgattg tctccgcttt
    36421 ggaatctgcc gaaaaagccg ttctattggg actgcccgaa gacaaaatca tcctgtcgtg
    36481 caaagtcagc gcggttcatg atttgattca ggtttaccgc gaactgggca gccgttgcgt
    36541 ctatccgctg catttgggtt tgaccgaagc cggtatgggc agcaaaggca ttgtcgcatc
    36601 aacggcggca ttatctgttt tgcttcaaga aggaattggc gacaccatcc gcatttcact
    36661 cactccggaa ccgggcagcc cgcgtactca ggaggtcgtc gtcgggcaag agattttaca
    36721 gactatgggt ttgcgctcgt ttacgccgat ggttaccgcc tgcccgggat gcgggcgtac
    36781 caccagtacc gtatttcaag agctggcaca agatgttcaa aattacctgc gccaaaaaat
    36841 gtctatatgg cgtacccttt atcccggggt tgaatccctg aacgttgccg taatgggctg
    36901 cgttgtcaat ggccccggag aaagcaaatt ggccgacatc ggaatcagcc ttcccggtac
    36961 gggggaaaca cccgtcgcac ctgtttatgt agatggggag cgcaaagtca cactgaaagg
    37021 taataatatt gcaagcgaat tcctagctat tgtagaggaa tatgttaaaa ccaattatgg
    37081 caaaaacagt tctaaacgca ataaaggaaa agtcatcccg atacagtctc tataaaacaa
    37141 atgccgtctg aacgtttcag acggcatttg tttctctcag atttcttatt aaagccgcaa
    37201 agaagcgcgg ttttccaaaa cttggtcgat caaaccatat tcttttgctt cttcggcaga
    37261 catgaaatta tcacggtcgg tgtcgcgctc caaatctgcc aaatcgcggc cgcaatgttt
    37321 cgccatcagg cggttgagtt tttctttgat tttcaacaac tcgcgtgcgt ggatttcaat
    37381 gtcggatgcc tgaccgccca agccgccgct gattaaaggc tggtgaatca taatccggct
    37441 gttgggcagg gcgaaacgtt tgcctttctc gcctgccgac aataagaacg cgcccatact
    37501 tgccgcctgc cccaagcaca aagtcgatac atcgggcttg atgaaattca tcgtgtcgta
    37561 aatcgacata ccggccgtta ccgagccgcc gggggaattg atgtagaaga aaatatcctt
    37621 atccggattc tcactttcca aaaacaacag ttgggcgacc accagattag cagactcatc
    37681 ggttaccggg ccaaccagaa atacgatgcg ctctttcaaa agccgggaat agatatcgaa
    37741 tgcacgctca ccgcgaccgc tctgctcgat aacggtaggg acaagatggt tatcaaaaga
    37801 catttcgtct cctttcatga tggaaaagca ccaaagcggg ctttaaaaac ggcttcggtg
    37861 ctttcaaaaa ctgccttcag acagcatttt cagaacaatc aggcttgcgc gcccatcact
    37921 tcgtcaaaag acaaagcttt tttgtttact ttggctttgc ccaaaacgaa atcaacaacg
    37981 ttgctttcta ctgccaaaga agtcggggct tgcaggcggg aagtatctgc gtagtaccag
    38041 tcaatcactt cttgaggatc ttcgtagctt tctgcgaagt tggcgacaac ggctttgatt
    38101 tgctcttcag tcggttccag tttgttttcg tcaaccagtt tggccaaaat cagacccaaa
    38161 gatacgcgac gttcggcttg ttctttgaac atatccaaag gcaaatccaa gttcgcggca
    38221 tcggtcatac cttggttaac gaagttttgt ttcatttcgt ttgccaggcg ggcggcttct
    38281 tcattgacca aagcaaccgg aactttcaac tctacggctt taatcagcgc gttcattacg
    38341 gattctttgg tttgttcgtt cacgcggcgt tccacttcgc ggcttacgtt tttcttcact
    38401 tcttcacgca ttttggcaac atcgccatcc gcaataccca aggcttttgc aaaatctgca
    38461 tcgacttcag gcagagtggg ctcggaaaca ttgttcagcg taatggtgaa cacggcagtt
    38521 ttaccggcaa catctttgcc gtggtattct tcagggaagt tgacggtaac gtctttactt
    38581 tcgcccgctt tcatgccgac tacgccggct tcaaattcag gcagcatttg acctgcgccc
    38641 aatacgaagg cgtagttttt ggatgcgccg ccggcaaaag gttcgccgtc gattttgcct
    38701 tcaaagtcga tgatgacgcg gtcgccgttt cgggcttcgc ggtcaacatg gttgaagcgg
    38761 gtacgttgtt tacgcaggat ttctacagtt tggtcgactt cggcatcgcc gacggaagcg
    38821 gttacttttt cgacctcttg tgcagacaaa tcgccgataa cgacttcggg gaacacttca
    38881 aaaatggcgg caactttgaa agactcttta tcgtcttgtt cttcaacgcc ttcaaaacgt
    38941 ggatagcctg ccactttcaa ctcttgggca acggcaacat cgtagaagcg gcgttgcacc
    39001 agctcgttga tcacgtcgtt ttgcgcgctc gcaccgtaca tttgggcaat catttttaaa
    39061 ggtgctttac ccggacggaa accgtcgatt tttgcacggc gttgggtttg tttcagtttt
    39121 ttatcggttt ctgcgttgat ttcggaccaa ggcagggaca acactacttt gcgttccaga
    39181 ttttctaaaa tttcaacagt tacgctcatc ataagccctt aaatttgttg tgttgataaa
    39241 atgataaacc ttcttccctg catggggaag caaacagcgc aacggtacga tatttgaacc
    39301 gcattgccgc aaaggggaaa ttttagctgg caagtatatc acaatgtttc gcctgaaaca
    39361 taatatgccg agacctttgc aaaattcccc taaaatcccc taaattccca ccaaggcatt
    39421 taggggattt cccatga
//
LOCUS       DBJGGI010000012        38014 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.012, whole genome shotgun sequence.
ACCESSION   DBJGGI010000012 DBJGGI010000000
VERSION     DBJGGI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38014
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.012"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            298..588
                     /locus_tag="ACSIGJ_000622"
     CDS             298..588
                     /locus_tag="ACSIGJ_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HHQ1329571.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKAGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
     gene            681..2315
                     /gene="groL"
                     /locus_tag="ACSIGJ_000623"
     CDS             681..2315
                     /gene="groL"
                     /locus_tag="ACSIGJ_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HHQ1329572.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGVLKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTVID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIGMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAVPDMGGMGGMGGMM"
     gene            3019..4554
                     /locus_tag="ACSIGJ_000624"
     CDS             3019..4554
                     /locus_tag="ACSIGJ_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HHQ1329573.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIIGGRLPELRRHLNALSSIRVGGLWTACVVFTVVMLGYMLYQ
                     DTAGLLEKNYGDYPDGFLNIFGWGMSAALIMFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     GEK"
     gene            4551..4643
                     /locus_tag="ACSIGJ_000625"
     CDS             4551..4643
                     /locus_tag="ACSIGJ_000625"
                     /inference="COORDINATES: protein motif:HMM:NF028261.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HHQ1329574.1"
                     /translation="MSTSAIVMMVAAIAVIWGGLLLSLLRLPEE"
     gene            complement(4679..5119)
                     /locus_tag="ACSIGJ_000626"
     CDS             complement(4679..5119)
                     /locus_tag="ACSIGJ_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329575.1"
                     /translation="MKHAPIILLLTASSAALLSAGCTALPSDRPPLRTLPKSAPIQPA
                     NPYSRPPSGTPDGAFSTRASNGRVLKSIVKNGVFDRFVDIYHPNGKLHSHTPVENGVA
                     QGYTEQGILRTRILYRGTGISSERKLWMRRARWNANGSRKTNAV"
     gene            complement(5313..6533)
                     /gene="lysA"
                     /locus_tag="ACSIGJ_000627"
     CDS             complement(5313..6533)
                     /gene="lysA"
                     /locus_tag="ACSIGJ_000627"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HHQ1329576.1"
                     /translation="MTLFCEQVAYPRLAEEFGTPLYVYSQSALTGAFENYQTAFAALN
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKIAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYKDEGVPDLGAYARAVQKLMGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKNIEPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNTRNRAAEVLVDGGGYKLIRRR
                     ETLEQQMANELACL"
     gene            complement(6544..6714)
                     /gene="lptM"
                     /locus_tag="ACSIGJ_000628"
                     /gene_synonym="yifL"
     CDS             complement(6544..6714)
                     /gene="lptM"
                     /locus_tag="ACSIGJ_000628"
                     /gene_synonym="yifL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS translocon maturation chaperone LptM"
                     /protein_id="HHQ1329577.1"
                     /translation="MKYGVFFAAATALLLSACGYKGGLYLPKEGDTARFGAIQTGLQL
                     QGKPQSAPPTQK"
     gene            6785..7108
                     /gene="cyaY"
                     /locus_tag="ACSIGJ_000629"
     CDS             6785..7108
                     /gene="cyaY"
                     /locus_tag="ACSIGJ_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HHQ1329578.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIAEL"
     gene            7141..7560
                     /locus_tag="ACSIGJ_000630"
     CDS             7141..7560
                     /locus_tag="ACSIGJ_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HHQ1329579.1"
                     /translation="MAQLPLYRTAEIGNFTVGTPKVLESFSKHIPYGVVFEDDGNTGY
                     FYAASQEGILDALHIYNVEDVSDKHIPNHVLILWDGACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSRKPT"
     gene            7582..8088
                     /gene="luxS"
                     /locus_tag="ACSIGJ_000631"
     CDS             7582..8088
                     /gene="luxS"
                     /locus_tag="ACSIGJ_000631"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HHQ1329580.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGAGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYLMHSLAEAQQIAQNVLARKVAVNRNGDLA
                     LDESLLNA"
     gene            8232..11024
                     /gene="polA"
                     /locus_tag="ACSIGJ_000632"
     CDS             8232..11024
                     /gene="polA"
                     /locus_tag="ACSIGJ_000632"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HHQ1329581.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALIGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVMEHAP
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHTELSDGIESLRRTAPKWAQLAVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEIPSEKRAEKATAPEKLDYQA
                     VTTEAQFAALLDKLAKADTIGIDTETTSLDAMNAELVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNNIAGDAMLASYI
                     IESHLGHGLDELSGRWLGLETITYESLCGKGAKQISFADVAIGQATEYAAQDADFALR
                     LEARLRAQMDDKQLEMYEKMELPVAQVLFEMECNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPPGSVIVSADYSQIELRIMAHLSGDKTLIAAFQSGEDVHRRTAA
                     EVFGTAPENVSPEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMINVRNWLSDGIGSKLVMQVHDELVLEVVETELDFVKEKLPQIMAKVDGGLLDV
                     PLVAEVGVGENWEEAH"
     gene            11586..12281
                     /locus_tag="ACSIGJ_000633"
     CDS             11586..12281
                     /locus_tag="ACSIGJ_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HHQ1329582.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     INGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVNISLLKRINNIVAKDDSLVPV
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPRFNEKLSAFYRTGDDTDISKFVYQNC
                     ISGIDYFGADEDI"
     gene            complement(12273..12383)
                     /locus_tag="ACSIGJ_000634"
                     /pseudo
     CDS             complement(12273..12383)
                     /locus_tag="ACSIGJ_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(12521..16927)
                     /gene="app"
                     /locus_tag="ACSIGJ_000635"
     CDS             complement(12521..16927)
                     /gene="app"
                     /locus_tag="ACSIGJ_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HHQ1329583.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQARAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALAGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFSYQIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGWKYADLNKYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPHQNGKYFFND
                     NNNGAGKIDAKHKHYSLPYRLKTRTVQLFNVSLSETAREPVYHAAGGVNSYRPRLNNG
                     ENISFIDKGKGELILTSNINQGAGGLYFEGNFTVSPKNNETWQGAGVHISDGSTVTWK
                     VNGVANDRLSKIGKGTLLVQAKGENQGSVSVGDGKVILDQQADDQGKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDITTTGNNNNLDSKKEIAYNGWFGEKDATKTNGRLNLNYQPEEADRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLGSGWSKMEGIPQGEIVWDNDWIDRTFKA
                     ENFHIQGGQAVVSRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTSCTEK
                     TITDDKVIASLSKTDIRGNVSLADHAHLNLTGLATLNGNLSAGGDTHYTVTRNATQNG
                     NLSLVGNAQATFNQATLNGNTSASDNASFNLSNNAVQNGSLTLSDNAKANVSHSALNG
                     NVSLADKAVFHFENSRFTGKISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSAADAPRRRSRRSLLSVTPPTSAESRFNTLTVNGKLNGQGTFRFMS
                     ELFGYRSGKLKLAESSEGTYTLAVNNTGNEPVSLEQLTVVEGKDNTPLSENLNFTLQN
                     EHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAGETEAALTAKQAQLAAKQQAE
                     KDNAQSLDALIAAGRDATEKAESVAEPARQAGGENAGIMQAEEEKKRVQADKDTALAK
                     QREAETRPATTAFPRARRARRDLPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVF
                     AVQDELDRVFAEDRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVG
                     ILFSHNRTENTFDDGIGNSARLAHGAVFGQYGIGRFDIGISAGAGFSSGSLSDGIRGK
                     IRRRVLHYGIQARYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRA
                     GIKADYSFKPAQHISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAE
                     IKGFTLSLHAAAAKGPQLEAQHSAGIKLGYRW"
     gene            17365..18711
                     /gene="mnmE"
                     /locus_tag="ACSIGJ_000636"
     CDS             17365..18711
                     /gene="mnmE"
                     /locus_tag="ACSIGJ_000636"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HHQ1329584.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPEP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMEMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASGRSAARLALRSLKGDFSRRIHGLV
                     EGLITLRMLVEAALDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRMILDTLPSDLKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLCEAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            complement(19604..22036)
                     /gene="hupB"
                     /locus_tag="ACSIGJ_000637"
     CDS             complement(19604..22036)
                     /gene="hupB"
                     /locus_tag="ACSIGJ_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HHQ1329585.1"
                     /translation="MHIPFKPVLAAAAIAQAFPAFAADPAPQSTQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDIEIKNDGYVYNPTDTGGPSKYLTYVATGVARSQPDPQEWVNKSTLFKL
                     GYNFNDQNRIGWIFEDSRTDRFTNELSNLWTGTTTSAATGDYRHRQDVSYRRRSGVEY
                     KNELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDLRGINGEVYHSFRHIRQNTAQWT
                     ADFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYAPKILASNQAKITMLIENR
                     SKYKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAH
                     AGFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPNLKAEKAK
                     NWELGLAGSGKAGNFKLSGFKTKYRDFIELTYMGVSSDDKNNPRYAPLSDGTALVSSP
                     VWQNQNRSAAWVKGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINAL
                     SPWTAVYSLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLF
                     DLSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSP
                     GRSYNFTIEAKF"
     gene            complement(22067..23152)
                     /locus_tag="ACSIGJ_000638"
     CDS             complement(22067..23152)
                     /locus_tag="ACSIGJ_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slam-dependent surface lipoprotein"
                     /protein_id="HHQ1329586.1"
                     /translation="MKYKALPLLPLAAALAACAGGGVAEPHVPVSIPTATPLPAGEVT
                     LSSDNGNIENINTAGAGSASDAPSRSRRSLDAAPQNTSGISIRQREVEKDYFGYKSKE
                     TSFIFKTPGGAQYALSSYADPITVSYSSPDFKIPDRHAGQRLADGSRIFICCSDSGAT
                     SYAEITKQDYMKFGAWIGPNGEIDLFAGGFPVGKTPISNSYYSSTPETALSKGKITYQ
                     VWGIRVKDGQFVTSSYTPPKNSGYYSSTPTNTPVLSFITANFNTQKLAGKILGNSDYG
                     PDVEIKEATISGLTFSGDATSGGKTGKLDGKFFGKFNGSYGNTETSIGGKITFDGDRS
                     LDTVFGGVSYKKELENSTDMSTTHLTK"
     gene            23395..25011
                     /locus_tag="ACSIGJ_000639"
     CDS             23395..25011
                     /locus_tag="ACSIGJ_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HHQ1329587.1"
                     /translation="MMRRVRRAWGLRRFCRRRPAVFFKEGKLKDSVYRVCRLCAALCV
                     WGAVGAYAAGLPDVRDDAAALRAQRAAAEGWAGMPPEGDSAANGGSRVIDGDFLLSRP
                     QLLEHVLRDALNGNQADLIASLADLYAKLPDYDAVLYGRARALLAKLAGRPAEAVARY
                     RALHRDNAADERVLLDLAAAEFDDFRLKSAGLHFAEAAKLDLPAPVLENVGRFRKKTE
                     GLTGWHFSGGISPSVNKNANNAAPQYCLQGGGSRICSVTGPVRAAGLDYEAGAEKLTA
                     LADNHYLLFRANVGGTSYYFSRKSAYDDAFGRAYLGWQYKNARQTVGVLPFYQAQLSG
                     SDGFDAKTKPPADRRLAPYMLAHGAGVQLAHSYRLSRRSQLSVSLERYRQRYREQGRA
                     ERNDGWQDGLYVSLARRLGGSATVFGGWQFARFVPKRETVGGAVNNAAYRRNAVHIGW
                     AQEWGGTGGLNSRIAASYAHRNYKGIAAFSTEAQRNREWNVSLALSHDKLSYKGIVPT
                     LNYRFGKTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(25072..26946)
                     /locus_tag="ACSIGJ_000640"
     CDS             complement(25072..26946)
                     /locus_tag="ACSIGJ_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HHQ1329588.1"
                     /translation="MPYFALFDDAVSRRAKLYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHSVLFADYEFGLPLMGMESERGGNIALHWFADCADTDAESWLARHSDGIPAGIST
                     PQSSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDEAGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQTD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIRAQALPDTSF
                     ADTLRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLNPCSGGLGFEGAFNV
                     VIRTLSLKPVSDGIVSGIGGTDSNAQARTAGQGGETPHPFEANPPYRGVYGVGSGIVI
                     DSDPAAEYRECGWKARFLNELRPDFGIFETLRAENRQCALLDRHLCRLKTSAQALNLP
                     LPDGCENQIKQYIADLPDGAFRIKALLASDGISLSHAVLNRLTDKQRVIISPTILPAQ
                     NYLRRFKTTHRAIFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKHRGQWLTPS
                     LDLDILNGIMRQAVSDEPQKYLHTNQVIETHITQKTLQGAEEIRLSNALRGVFAAVLA
                     "
     gene            27117..28046
                     /gene="ppk2"
                     /locus_tag="ACSIGJ_000641"
     CDS             27117..28046
                     /gene="ppk2"
                     /locus_tag="ACSIGJ_000641"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HHQ1329589.1"
                     /translation="MTDRQLQPFENVELGEKQDQLQVFEKAVLEHEGKGSAEDSGTAP
                     LPENYPCRKRMRRAVYEAEKAKLQIELLKVQSRVKDSGQRIVSLFEGRDAAGKGGTIK
                     RFMEHLNPRGARVVALEKPTTTERGQWYFQRYIQNLPTAGEMVFFDRSWYNRAGVERV
                     TGFCEPNEYMLFMCQAPELERMLVASGIHLFKFWFSVSREEQLRRFISRRDDALKHWK
                     LSPVDIQSLDRWDDYTEAKNAMFFHTHTGDAPWVIIRSDDKKRARLNCIRYFLHQLDY
                     PGKDVKAIGKVDDKIVLVPDTRYKEKTIDVGHD"
     gene            complement(28128..29559)
                     /gene="aldA"
                     /locus_tag="ACSIGJ_000642"
                     /pseudo
     CDS             complement(28128..29559)
                     /gene="aldA"
                     /locus_tag="ACSIGJ_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(29667..30582)
                     /locus_tag="ACSIGJ_000643"
                     /pseudo
     CDS             complement(29667..30582)
                     /locus_tag="ACSIGJ_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            30755..31555
                     /locus_tag="ACSIGJ_000644"
     CDS             30755..31555
                     /locus_tag="ACSIGJ_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHQ1329590.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTQLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            31603..32379
                     /gene="mlaE"
                     /locus_tag="ACSIGJ_000645"
     CDS             31603..32379
                     /gene="mlaE"
                     /locus_tag="ACSIGJ_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HHQ1329591.1"
                     /translation="MNFIRSVGAKTLGLIQPFGSITLFLLNILAKSGTAFARPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTGQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLGLDSGIFWPQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCIPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            32430..32924
                     /gene="mlaD"
                     /locus_tag="ACSIGJ_000646"
     CDS             32430..32924
                     /gene="mlaD"
                     /locus_tag="ACSIGJ_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HHQ1329592.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNAEGGNAE
                     KAAE"
     gene            32955..33545
                     /locus_tag="ACSIGJ_000647"
     CDS             32955..33545
                     /locus_tag="ACSIGJ_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaC/ttg2D family ABC transporter
                     substrate-binding protein"
                     /protein_id="HHQ1329593.1"
                     /translation="MKKSSFISALGIGILSIGMAFASPADAVGQIRQNATQVLTILKS
                     GDAASARPKAEAYAVPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKFKNATVNVKDNPIVNKGGKEIVVRAEVGIPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGIDGLIAELKAKNGGK"
     gene            33596..33874
                     /locus_tag="ACSIGJ_000648"
     CDS             33596..33874
                     /locus_tag="ACSIGJ_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HHQ1329594.1"
                     /translation="MHTELKNGTLHIGGDITVKTLTGDAFGRFRQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            33871..34698
                     /locus_tag="ACSIGJ_000649"
     CDS             33871..34698
                     /locus_tag="ACSIGJ_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0120010 - intermembrane phospholipid
                     transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HHQ1329595.1"
                     /translation="MKKTAYAILLLIGFASAPAFAETRPADPYEGYNRAVSKFNDQAD
                     RYIFAPAARGYRKVTPKPVRAGVSNFFNNLRDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGVPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYPPKNIVFHTPAGRWGTTAAAAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAEPAVHEDSVSETQAEAAGEAETQPGTQP"
     gene            34823..35185
                     /locus_tag="ACSIGJ_000650"
     CDS             34823..35185
                     /locus_tag="ACSIGJ_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HHQ1329596.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            35185..35568
                     /locus_tag="ACSIGJ_000651"
     CDS             35185..35568
                     /locus_tag="ACSIGJ_000651"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HHQ1329597.1"
                     /translation="MKLTVRNYHLDGYGHVNNARYLEFLEEARWAFFEKRGLMHELAG
                     LILIVARIDIRYSRPAVEGDVLQFSCRLKTPGMRRIVLTQTITLPNGKTAAEADITLM
                     PVHAATQRTVSLPATLARALEALSE"
     gene            35565..36053
                     /locus_tag="ACSIGJ_000652"
     CDS             35565..36053
                     /locus_tag="ACSIGJ_000652"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HHQ1329598.1"
                     /translation="MKKILTAAAVALIGILLATVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKVIKTANDYKNKDFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVIYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(36134..36262)
                     /locus_tag="ACSIGJ_000653"
     CDS             complement(36134..36262)
                     /locus_tag="ACSIGJ_000653"
                     /inference="COORDINATES: protein motif:HMM:NF025953.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HHQ1329599.1"
                     /translation="MAGVTVGRGSVVGAGAVITKDIPPYSLAAGNPAVVKKNLPEG"
     gene            complement(36268..36630)
                     /locus_tag="ACSIGJ_000654"
     CDS             complement(36268..36630)
                     /locus_tag="ACSIGJ_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HHQ1329600.1"
                     /translation="MESKFFFLLLRFAGSVLPPSHMRGIGIIGRRVLGFLARRISPHI
                     GRGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMGPECLFYSTNHKFDREKNALRA
                     TRKSVRLRWRTMSGRGAG"
     gene            36765..36980
                     /gene="rpmE"
                     /locus_tag="ACSIGJ_000655"
     CDS             36765..36980
                     /gene="rpmE"
                     /locus_tag="ACSIGJ_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HHQ1329601.1"
                     /translation="MKQGIHPNYREVNVTCSCGNKFVTKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
     gene            37245..37873
                     /locus_tag="ACSIGJ_000656"
                     /pseudo
     CDS             37245..37873
                     /locus_tag="ACSIGJ_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
BASE COUNT         8345 a         9883 c        10633 g         9153 t
ORIGIN      
        1 cggcgaagcc gcaggcagta cagatagtac ggaaccgatt cacttggtgc ttcagcacct
       61 tagggaatcg ttctctttga gctaaggcga ggcaacgccg taccagttta aatttagtcc
      121 actataaaaa acggcggaaa taaatttttt tccgcctcac ttgaattttc ccgcacacac
      181 cctaattttg ccgacttgta cgggcgggcc gttaggcggc agcccggttt ccacttcaat
      241 ttgtccgaac cgttcgggca gatgattgtt ttcaaaccat tttatcggag cataaatatg
      301 accatccgtc ctttacacga ccgcgtcgtc gtcaaacgct tggaagctga agaaaaaacc
      361 gcatcgggca tcgtcctgcc gggtgcggcc gccgaaaaac ccgatatggg cgaagtcatc
      421 gccgtgggcg cgggcaaaat cggcaaagac ggcgcgcgcc gtccgctgga tgtcaaagcc
      481 ggcgacaaaa tcatcttcgg caaatacagc ggccaaaccg taaaagccga cggcgaagag
      541 ctgttggtaa tgcgcgaaga agatattttc ggcatcgttg aaaaataaat acggacacga
      601 tgccgtctga aacggcaaac cgccttcaga cggcataaac ggttttatca gacagtttta
      661 atgatttttg gagaattgaa atggcagcaa aagacgtaca attcggcaat gaagtccgcc
      721 aaaaaatggt aaacggcgtg aatattttgg caaacgccgt gcgcgtaacc ttgggcccca
      781 aaggccgcaa cgtggtggtt gaccgcgctt tcggcggccc gcacatcacc aaagacggcg
      841 taaccgtcgc caaagaaatc gaactgaaag acaagtttga aaatatgggc gcgcaaatgg
      901 taaaagaagt cgcgtccaaa accaacgacg tagccggcga cggtacgact accgccaccg
      961 tattggcaca atccatcgtt gccgaaggca tgaaatacgt taccgccggc atgaacccga
     1021 ccgatctgaa acgcggcatc gacaaagccg ttgccgcttt ggttgaagag ctgaaaaaca
     1081 tcgccaaacc ttgcgatact tccaaagaaa tcgcccaagt cggctcgatt tccgccaact
     1141 ccgacgaaca agtcggcgcg attatcgccg aagcgatgga aaaagtcggc aaagaaggcg
     1201 tgattaccgt tgaagacggc aaatctttgg aaaacgagct ggacgtggtt gaaggtatgc
     1261 agttcgaccg cggctacctg tccccttact ttatcaacga cgcggaaaaa caaatcgccg
     1321 gtctggacaa tccgtttgtt ttgctgttcg acaaaaaaat cagcaacatc cgcgacctgc
     1381 tgcccgtgtt ggaacaagtg gcgaaagcca gccgcccgct gttgattatc gctgaagacg
     1441 tagaaggcga agccttggcg actttggtcg tgaacaacat ccgcggcgtc ctgaaaaccg
     1501 ttgccgtcaa agcccccggc ttcggcgacc gccgcaaagc gatgctgcaa gacatcgcca
     1561 tcctgaccgg cggcgtagtg atttccgaag aagtcggcct gtctttggaa aaagcgactt
     1621 tggacgactt gggtcaagcc aaacgcatcg aaatcggtaa agaaaacact accgtcatcg
     1681 acggcttcgg cgacgcagcc caaatcgaag cgcgtgttgc cgaaatccgc caacaaatcg
     1741 aaaccgcgac cagcgattac gacaaagaaa aactgcaaga gcgcgttgcc aaactggcag
     1801 gaggcgtggc agtgatcaaa gtcggcgcgg cgaccgaagt cgaaatgaaa gagaaaaaag
     1861 accgcgtgga agacgcgctg cacgctaccc gcgcagccgt tgaagaaggc gtggttgcag
     1921 gcggcggcgt agccctgttg cgcgcccgcg ccgctttgga aaacctgcac accggcaatg
     1981 ccgaccaaga cgcaggcgta caaatcgtat tgcgcgccgt tgagtctccg ctgcgccaaa
     2041 tcgttgccaa cgcaggcgga gaacccagcg tggtggtgaa caaagtgttg gaaggcaaag
     2101 gcaactacgg ttacaacgca ggctccggcg aatacggcga catgatcgga atgggcgtac
     2161 tcgaccctgc caaagtaacc cgttccgcgc tgcaacacgc cgcgtctatc gccggtctga
     2221 tgctgacgac cgactgcatg attgccgaaa tccctgaaga aaaaccggct gtgcccgata
     2281 tggggggaat gggcggtatg ggcggcatga tgtaagcaat gccgtctgaa accttcagac
     2341 aacaagccgc acggtcaacg ccgtgcggtt tttttgcgaa taagtgcggc taaggcgcaa
     2401 accttaaaat tatagcggat taacaaaaac cggtacgtcg ttgccccgcc ccggctcaaa
     2461 gggaacgatt ccctaaggcg cccaagcacc gggcgaaccg gttccgtacc attcgtactg
     2521 cctgcggctg gccgccttgt cctgattttt gttaatccgc tatatttccc gccatcccaa
     2581 aaacgaagag cggcaggaat ttatcggaaa aacagcacct ctccgccgtc attcccgcga
     2641 aagcgggaat ctaggttcgt ccggtttcgg ttatttccga tagattcctg ccgcgttggg
     2701 ggtctggatt cccgtctgcg cgggaatgac gggactttag gtttctgttt ttgtttttct
     2761 gtttttgcgg gaatgacggg gaagttggcg gttgtttctt atggagtggt ttttgtattt
     2821 ggataaaaaa tttttcgttt tcaagccttc accgcttgcc atcggcgtta aaatttttta
     2881 cgataagcac atagattgta aacaatcggc cacaagccgg tttgtttttt cagaagacat
     2941 tatccctgtc agacgctgtt tctatatatg tttgcctata acggcttgtt tttaataaat
     3001 aattcaagag gtaccaacgt gtctgattcc aaaacgaaag aacgcgccac attcggcacg
     3061 cgccgcgcgt ttatgattgc cgccatcggg tccgccgtcg gcttgggcaa tatttggcgt
     3121 ttcccctata ttgcttttga aaacggcggc ggcgcattca tcctgcccta tctggtagcg
     3181 cttctgacgg cgggcatccc gctgctgctg ctcgattatg ccatcggcca ccgttaccgg
     3241 ggttctgcgc ccttggcttt ccgccgcctc ggacgctggt tcgagccggt cggctggtgg
     3301 aacgtgatga ccaatatcgt catctgcatc tattacgcag tgattatcgg ttgggcggca
     3361 agctatacct attattcggt caacgccgcc tggggtgcgg atccgcaggg ttttttcttt
     3421 aaggacttcc tgcaaatggc gggcccggaa gccttgggtt tggattttgt cggcaaagtc
     3481 gccggccctt tggcgggcgt gtgggttttt accgccgcca ttatggcttt gggcgtgcaa
     3541 aagggcgtgg cgcgcgcctc gtcgttcttt atgccgctgc ttttggtgat gtttttgatt
     3601 atggtcggca tttcgctgac gctgccgggt gcggcaaagg gcttggacgc attgtttacg
     3661 cccgactggt cgaaactcgc cgattccaag gtctgggtgg cggcatacgg gcagattttc
     3721 ttttcgcttt ccatctgctt cggcattatg gttacctatt cttcttattt gaagaaaaaa
     3781 accgacttgg gcggaacggg gctggtggtc ggttttgcca acagcagctt cgaactgctc
     3841 gcgggcatcg gcgtgtttgc cgcattgggc tttatggcgc aggcgggcgg caaggcggtc
     3901 aacgaggttg cctccggcgg catcggtttg gcgtttatcg cctttccgac cattatcaac
     3961 caggcgccga tgggctggct gatcggcata ttgtttttcg gttcgctggt gttcgccggc
     4021 gttacgtcga tgatttccat ccttgaagtg attgtggcgg cgattcagga caagctgaac
     4081 atcgggcgcg tcaacgccac gctgctggtc tgcattccga tgggcattgt ttccacgctg
     4141 ctgttcggta cggcgacggg gctgcctgtt ttggacgtga tggacaaatt cgtcaacacc
     4201 tacggcattg ttgccgccgg ttttgtttat gttgccgcca tcatcatcgg cggcaggttg
     4261 ccggaattac gcaggcacct gaacgccttg tcctccatcc gcgtcggcgg cttgtggacg
     4321 gcctgcgtcg tgtttaccgt cgtgatgctc ggctatatgc tgtatcagga caccgccgga
     4381 ttgttggaaa aaaactacgg cgattatccg gatggtttcc tcaatatttt cggctggggg
     4441 atgtcggcgg cgttaatcat gttcgggctg ctgctgtcgc tgctgccttg gaaacacggt
     4501 caggatttta atgtcaaaga cgaacacgaa catgaacaag gaggagaaaa atgagtactt
     4561 ccgccattgt gatgatggtt gccgccatcg cggtgatttg gggcgggctg ctgctttccc
     4621 tgttaaggct gccggaagag taagccttta gaacgttaaa aatgccgtct gaaccgcttc
     4681 agacggcatt tgttttacgg ctgccattcg cgttccacct tgcccgacgc atccaaagtt
     4741 tgcgctcgga cgatatgccc gtcccccggt acaggatgcg cgtccgcaat atgccctgtt
     4801 cggtatagcc ttgcgccacg ccgttttcaa ccggcgtgtg cgaatgcagt ttgccgtttg
     4861 gatggtaaat atcgacaaag cggtcgaaca cgccgttttt gacgatgctt ttcaagacgc
     4921 gcccgtttga ggcgcgggtg ctgaatgcgc cgtccggcgt gcctgaaggc ggccggctgt
     4981 atgggttggc gggttggatg ggcgcgcttt tcggcagggt gcgaagcggc ggccggtcag
     5041 agggaagggc ggtgcagccc gccgaaagta gggcggcgga cgaagcggta agcaggagga
     5101 taatcggagc gtgcttcatt tcaattcctt agatattgca ccccataaaa ggggcgcgta
     5161 atttttttca acaattccct tcgtcagtcg ggcgagacgg ggatttgttg cgtcgtcatt
     5221 tgaaaacagg ctgtgtttga atattccgcc aagatgtcac tttaaacgga caatgccgtc
     5281 tgaaggcttc agacggcatt ttgatgttcg gcttataggc aggcgagttc gtttgccatt
     5341 tgctgttcca aggtttcgcg ccggcggatg agtttgtatc cgccgccgtc aaccaacacc
     5401 tccgccgcac ggttgcgcgt gttgtaattg ctagccatac tggccccgta tgcgcccgcg
     5461 ctgcggataa gcagcaaatc gccttcttcg caggcgatgg tgcggtcttt gccgaggaag
     5521 tcgccggttt cacaaatcgg gccgacgatg ttggcggtca gaggctcaat gtttttggtt
     5581 tcaaccgctt cgatgtggtg gtaggcatcg tatagggctg ggcgcatcaa atcgttcatc
     5641 gccgcatcga ccatcacaaa gtttttctct tcaccgtgtt tgacaaattc gacgcgcgtc
     5701 agcaatgcac ctgcgttgcc gaccaagctg cggcctggct caagaatgag tttcagacgg
     5761 cgtgtcccca tcagtttttg aaccgctcgg gcatacgcac ccaaatcggg gacgccttcg
     5821 tctttgtaaa caatgccgac gccgccgcct aagtccaaat gttccaaaac aatgccttcg
     5881 gcggcaagag cgtcaaccaa aatcaaaatg cgttcgcagg cttcgaccag tgggcttaag
     5941 tcggtcagtt gcgaaccgat gtggcagtcg atgccgatga ttttcaaatt gggctgttgt
     6001 gcggcatggc ggtaggcttc gagcgcgtcg gcgtaggcga tgccgaattt gttggctttc
     6061 agacctgtgg agatgtaggg atgggttttt gcatcgacat cgggattgac gcgcagggag
     6121 acgggcgcgg ttttgcccaa acgcgcggca attttctgaa tgcggtcgat ttcggggatg
     6181 ctttccatat tgaagcattt tacgccggca ttcagcgcga actcgatttc cgcctcgctt
     6241 ttgcctacgc cggaaaaaat cgttttcgcc gcatcgccgc ctgccgccaa aacgcgtgcc
     6301 aattcgccgc ccgacacaat gtcaaaaccg ctgcccaaag aagcaaagtg tttgataatg
     6361 ctcaggttgc cgtttgcctt gacggcgtag cagacaagcg ggttcaaagc ggcaaaggcg
     6421 gtttgatagt tttcaaatgc tccggtcagc gcggattggc tgtacacata aagcggcgtg
     6481 ccgaattctt cggcaaggcg ggggtaggcg acttgttcgc aaaacagggt catattttcg
     6541 ttttcatttt tgggttggtg gggcggattg cggtttgcct tgaagctgca aaccggtttg
     6601 gattgcgccg aaacgcgccg tatcgccttc tttgggcagg tagaggccgc ctttgtaacc
     6661 gcaggcggaa agcaggaggg cggttgccgc cgcaaaaaat acgccgtatt tcatcggtaa
     6721 acttccttca taagcgcgaa tgtggcaaga ttcggcatct taaacaaaaa acacgcaaaa
     6781 agctatgatg accgaaagcg agtttatccg cgcgagcgaa gcattatttg aacacatcga
     6841 agaccaaatc gacgaaaacg gctgggattt cgactgccgg tttgccggaa acgtcctgac
     6901 catcgaagcc ggagacggca cgcaaatcat cgtcaaccgc cacacgccca accaagaatt
     6961 gtggattgcc gcaaaaagcg gcggctacca tttcgccgaa caaaacggca aatggctggc
     7021 aacgcgcgac agccgcgatt tttacgacgt tttaaacgaa gccctgagcg cggcttcggg
     7081 cgaagcggtt gagattgccg aattgtgatt caattttcga tataaaaaga ttgttaccca
     7141 atggcacaat tacctctata tcggactgcc gaaatcggaa attttactgt cggcacgcct
     7201 aaagttttag aatcattttc caaacatatc ccttatggtg ttgtctttga agatgatggc
     7261 aacacgggct acttctatgc cgcttcgcaa gaggggattt tggatgcctt gcacatctac
     7321 aatgtcgaag atgtatccga caaacatatc cccaatcatg tcttgatttt atgggatggc
     7381 gcctgcacca tagccgcatt gtgtatcaac gactacatcc atgccgtcta tgattttgtc
     7441 gaacaggcag gatattgccg caacggcttc cctgaagcag gcggcgaatg ggtaaaagtc
     7501 gaaaaccgcg tcttggacga tgaattgctg gacaaaatcc tatcccgaaa acctacataa
     7561 cccgcacaaa aggataccca aatgcccctg ttagacagtt tcaaagtcga tcacacccgt
     7621 atgcacgccc ctgccgtacg cgtggcgaaa accatgacca cgcccaaagg cgacaccatt
     7681 actgtattcg acctgcgctt ttgcatcccc aataaagaaa tcctgcccga aaaaggcatc
     7741 cacacgctgg agcatttgtt tgccggcttt atgcgcgacc acttgaacgg cgccggcgtg
     7801 gaaatcatcg acatttcccc gatgggctgc cgcaccggtt tttatatgag ccttatcggc
     7861 acgccttccg aacagcaggt tgccgatgcg tggttggctt cgatgcagga tgttttgaat
     7921 gtcaaagacc aaagtaaaat ccccgagttg aacgaatacc aatgcggcac ttatctgatg
     7981 cactcgctcg ccgaagcgca acaaatcgcg caaaacgtgt tggcgcgcaa agtggcggtt
     8041 aaccgaaacg gcgatttggc tttggatgaa agcctgctga acgcttaatc cgccgcaaat
     8101 gctgtctgaa caagggtttc agacggcatt tgccttttcc gttataatcc ggggttgtac
     8161 gggggcgggt tttaagccgg catcgtcctt ccctattttt ctgtccctta tcggtttcaa
     8221 gcgggttttt tatgtccaac agacctacac tcctcctcgt tgacggctct tcctatctct
     8281 accgcgcgta tcacgcgatg gggcaaaacc tgactgcgcc tgacggtgcg ccgacgggtg
     8341 cgctgtatgg cgtattgaat atgttgcgcc gtttgcggtc ggaatatccg cacgattatt
     8401 gcgcggtggt ttttgatgcg aaaggcaaaa atttccgcca tcaaatgttt gaagaataca
     8461 aggcgacgcg cccgccgatg ccggacgatt tgcgcccgca ggcggaagcc ctgccggatt
     8521 tggtgcgcct gacgggctgg ccggtattgg tgattgggca agtggaggcg gacgatgtga
     8581 tcggcacgct ggcgaaacag ggggcggaac atggtttgcg agtcattgtt tcgacgggcg
     8641 ataaggatat ggcgcagttg gtggatgagc gcgttacgct ggtgaacacg atgagcggag
     8701 aaacgctgga tattgaaggc gtgaaggcaa aattcggcgt tcgccccgac caaatccgcg
     8761 attatctcgc gctgatcggc gacaaggtgg acaacgtacc gggcgtggaa aaatgcggcc
     8821 cgaaaacggc ggtgaaatgg ctggaagcct acggttcgtt gcaaggcgtg atggaacacg
     8881 ctccggaaat caaaggcaag gtcggcgaaa acctgcaagc cgcgctgccc caactgccgc
     8941 tgtcgtatga tttggtcacg attaaaaccg atgtggactt gcacaccgag ctttcagacg
     9001 gcatcgaaag cctgcgccgt accgcgccga aatgggcgca gcttgcggtt gatttcaaac
     9061 gctggggctt ccgtacttgg ctgaaagaag cggaatcaaa catgaatacc ggctcgaccg
     9121 atgatttgtt cggtagcgac agcatcggcg agcaggcggc tttgaatgcg gaaataccgt
     9181 ctgaaaaacg agctgaaaaa gccaccgccc ctgaaaaact ggattatcaa gccgttacca
     9241 ccgaagctca gtttgccgcc ttgttggaca aactagcgaa ggcggacaca atcggcattg
     9301 atacggaaac cacgtcatta gatgcgatga atgccgagct ggtcggcatc agcattgcgt
     9361 tccaagcagg cgaagcggtt tacatccccg taggccacag cctgactgcc gcgcccgaac
     9421 agcttgattt gcaagacgta ttaggccgtc tgaagccgca tttggaaaac cccgccctga
     9481 aaaaaatcgg tcaaaacctc aaatacgacc aacacgtttt cgccaactac ggcatcgcgt
     9541 taaacaacat tgccggcgac gcgatgctcg cttcctacat catcgagagc catctcggac
     9601 acggcttgga cgaattgtcc ggacgctggc tcggtttaga aaccattacc tacgaatcgc
     9661 tgtgcggcaa aggcgcgaag caaatcagtt ttgccgatgt cgccatcggg caggcgaccg
     9721 aatacgccgc ccaagatgcc gatttcgccc tgcgcctcga agcgcgcctg cgcgcgcaaa
     9781 tggacgacaa acaacttgaa atgtatgaaa aaatggagct tcccgtcgcg caggtattgt
     9841 ttgaaatgga atgcaacggc gtgcaaatcg accgcgccga actcgcccgc caaagcgcgg
     9901 aactcggcgc ggagctgatg aagctcgaac aggaagccta cgccgctgca ggtcaaccgt
     9961 ttaacctcaa ctcgcccaaa cagctgcaag aaatcctgtt cgacaaaatg ggcatcccga
    10021 caaaagggct gaaaaaaacc gccaaaggcg gcatttccac caacgaagcc gtgctcgaac
    10081 agctcgcgcc cgactacccc ctgcccaaaa tcatcctgca aaaccgcagc ctggcgaaac
    10141 tcaaatccac ctacaccgac aaactgcccg aaatgatttc gccccgggat aaccgcgtgc
    10201 ataccaccta tgcccaagcc gtcgccatta ccggccgcct cgccagcaac aaccccaacc
    10261 tgcaaaacat ccccattcgc accgccgaag gccgccgcgt gcgccgcgcc ttcactgcac
    10321 cgccgggcag cgtcatcgtt tccgccgact attcccaaat cgagctgcgc attatggcgc
    10381 acctctccgg tgacaaaacc ctcattgccg catttcaaag cggcgaagac gtacaccgcc
    10441 gcaccgccgc cgaagtgttc ggcactgcgc ccgaaaacgt ctcgcccgag caacgccgct
    10501 acgccaaaac catcaacttc ggcttaatct acggtatggg gcaatacggt ttggcaaaat
    10561 cattgggcat cgacaacctt tccgccaaaa actttatcga ccgctacttc gcccgttatc
    10621 ccggcgtcgc cgaatacatg cagcgcacca aagaacaagc cgccgcccaa ggctacgtcg
    10681 aaaccctgtt cggcagaagg ctctatttgc ccgacatccg caacaaaaac gccaacgccc
    10741 gcgcaggagc cgagcgtgcc gccatcaacg cccccatgca aggcaccgct tccgacctca
    10801 tcaaacgcgc catgataaac gtgcgaaact ggctttcaga cggcatcggc agcaaactgg
    10861 tcatgcaggt gcatgacgaa ctggtgctgg aagtcgttga aaccgaactg gattttgtca
    10921 aagaaaaact gccgcagatt atggcgaaag tggacggcgg attattggat gtaccgctgg
    10981 tggctgaggt tggcgtaggg gagaattggg aagaggcaca ttgagaatgc acaagtattg
    11041 aactgttacg ttttgattta tcaaaattta aggaagtaaa atacccgatt cgaatgattg
    11101 gaaatcaata gcaagccgta ggtcggatac ttgtatccga caaaagcctg ccatctcaaa
    11161 tagcagtcgg attcgagaat ccgacctgcc aaaccgggcg cggacgctcc ggtcggcagt
    11221 tagcgcacaa atcgaacaga acatcacaaa aaagcctgat tcggattttc caatcgggct
    11281 tttttgcgcc cgttttgtca tcccgtgaaa tatccgcata acaaaaataa tgcaaaatca
    11341 tgacaaaacc ggcgcgaggt tacacaaacg gatgaaatca accgatattc aaacacagcc
    11401 atttttagcg cattttcagc gtatctttat gcggaaaatt tcgtgaacag gttttttgca
    11461 caggcttccg ccgcgtttcg cgggatggga aaccgtatta gaaaacggac gcacgcaaca
    11521 taaaccccga aagtgatgat aagatgatga tttaacgtac tgctttaatt atttaaggaa
    11581 ttatcgtgtt tcccgacaaa tacaagttga gtttggaaga aaatattttt ctggcaaaga
    11641 aagtattggt tgctcaaatt cacaacctca gccgttttga gaattgtcag acgaccttgt
    11701 tgcagaccga gcaaattatc aatggcaaaa atgtagcctc cgcgtcactg gaagacatcc
    11761 aaaccatctt gaacctgaaa cgtgcctatc aatatgtgat ttcgcatatt tcaaacggcg
    11821 aaccggtcaa tatttcactc cttaaaagaa tcaacaacat tgttgccaag gacgattctt
    11881 tggtacccgt ggatttccgt accggttcgg tcggcgtaac gctattggac ggttcccgtc
    11941 atgccccgaa tccggtgaag gaaattgaag tggcccgcgt gttgcaaaat atcggactgc
    12001 aaagcggttc gacgacggaa gcagccgttc gtttcatgct ctattgtatg cggcagcagg
    12061 ttttttggga cggcaacaaa cggacggcaa ccctatttgc caacggcctg atgatggcgg
    12121 ggggctgcgg tatcctggaa atctccgaaa tgcagatgcc gcgattcaat gaaaaactat
    12181 ccgcattcta tcgcaccggc gacgataccg atatttccaa gtttgtgtat caaaattgta
    12241 tatcgggcat agactatttc ggcgcggacg aagatatata gtggattaaa tttaaaccgg
    12301 tacggcgttg cctcgccttg ccgtactatt tttactgtct gcggcttcgc cgccttgtcc
    12361 tgatttaaat ttaatccact atagatttcc ccgactcgcc gaacatgggc taaaaatcaa
    12421 tttgacggtt atcagacgat ggagtaggca caaggtggca gaaaaagggg tttgacagtg
    12481 cacggcggca tcgtcaaacc cctttcggca tatccggcgg ttaccagcgg tagcctaatt
    12541 tgatgcccgc gctgtgctgc gcttccaatt gcggcccctt ggcggcggca gcgtggaggg
    12601 acagcgtgaa acctttgatt tcggcgttta cgccccattc cgcactgcgg gttttgccga
    12661 aatcctgcgc caatacggcg gtattgacgc gcgttcggac tttgccggaa gcggcatcgg
    12721 tataggacag gctcaaataa ggcgtgatgg aaatgtgttg cgccggtttg aatgaataat
    12781 ctgccttaat gcccgcgcgg tagcggttga atgcaaggcc cggggtggcg atattgacgt
    12841 tttcgtatcg gtaatccgct ttttggacga aatagcgcgt tgcgccgatg tgcggttcga
    12901 tgccgaatcc gccgaaacct gcgcggtatc ttgcctgaat gccgtaatgc agcacgcggc
    12961 ggcggatttt gcctctgatg ccgtctgaaa ggctgccgct actaaaaccc gcgcccgcgc
    13021 tgatgccgat gtcgaacctg ccgatgccgt attgcccgaa aacggcaccg tgggcaagcc
    13081 gtgccgagtt gccgatgccg tcgtcgaagg tgttttcggt ccggttgtgc gaaaacagga
    13141 tgccgacgcg cccgctgccg aggtttttct gcataccgat ttggcgcagg tcggtttgtt
    13201 ggcggtaggc gcggaaatct tgcgaacggt agtgtttggt gtcccggatg ccgcttgtcc
    13261 aaacggcgtt gcggcggtct tcggcaaaca cgcggtccaa ttcgtcctgt acggcgaaaa
    13321 cgctgttgag cgtggcggaa aattcactca aaccgctatt ggcataacgg ctgatcaggt
    13381 cgcgctgcgg ttggggttgc ggttggggct gcggttgcgg caaatcccgg cgggcgcggc
    13441 gggcgcgggg gaaggcggtg gtagccggcc gggtttccgc ttcgcgctgt ttcgccaagg
    13501 cggtgtcttt atccgcctgc acccgttttt tctcttcctc cgcctgcata atgccggcat
    13561 tttccccgcc tgcctgccgg gccggttcgg caacgctttc tgccttttcg gtggcatcgc
    13621 gcccggccgc aatcagcgcg tcaaggcttt gcgcgttgtc tttttccgcc tgttgtttgg
    13681 cggcaagttg tgcctgtttt gccgtcaagg cggcctctgt ttctcccgcc ttgccgagtt
    13741 tgtcggaaag ctcttgttct ttgaccggat tatgcaggcg gaactcgccg tctttgcgga
    13801 taagctgata acgccatgcg ccggcatcga cgtgttcgtt ttgcagggtg aaattaagat
    13861 tttcggacag cggtgtgttg tcttttcctt ccactaccgt caattgctcg agacttacgg
    13921 gttcgttgcc ggtattgttg acagccaagg tgtaagtgcc ttcggaactt tccgccagct
    13981 tcaatttgcc gctgcggtag ccgaagagtt ccgacataaa gcggaatgtt ccctgaccgt
    14041 tcaatttgcc gtttaccgtc agcgtgttga aacgggattc tgccgaagtt ggcggcgtaa
    14101 cggataatag ggaacggcgc gaacggcggc gcggcgcatc tgccgcactg ccggtttgcg
    14161 cgcctgccgc atcgtgtcga taggcggaat tgagtgtaat ggtggcgttg tcaaggttta
    14221 aattgcctaa ttccgtgccc gacggcagcg tccattcgct gtcttttaag tgtaatgccg
    14281 tatccttgcc gccgctgatt tttccggtaa agcggctgtt ttcaaaatgg aatactgcct
    14341 tatcggctag ggagacattg ccgttgagtg cggaatggct tacgtttgcc ttagcgttgt
    14401 cggaaagcgt cagactgccg ttttgtacgg cgttgttgct tagattaaat gaagcattgt
    14461 ccgaagccga tgtgttgccg tttaatgtgg cttgattaaa tgttgcttgg gcattgccca
    14521 cgaggctaag gttgccgttt tgggtggcgt tgcgcgtaac cgtatagtgc gtgtctccgc
    14581 ctgcactaag attgccgttg agtgtggcaa gtcctgtgag atttaaatga gcgtgatcgg
    14641 caaggctgac attgcctctg atgtcggtct tgctcaatga agcaatcact ttatcgtcgg
    14701 taatggtttt ttcggtacaa cttgtcagac ccgtccagtc cgaacgtgta cagattgtgt
    14761 ggctttgatg cggtgcgaca ccgaaaactg cttgggcgtg attgcttaaa tgccaatcgc
    14821 cttccacttt ggcaacattg cgggaaacca ccgcttgtcc gccctgaata tggaagtttt
    14881 ccgctttaaa tgtgcggtcg atccaatcgt tgtcccacac gatttctcct tgtgggatac
    14941 cttccatttt tgaccacccg cttcctaaat gattgtaggc gtgcggtgtc ggtctgccgc
    15001 tgaaaaacag tttgccgttt gtttgcgtga tattgccgtt taaatttgtt ccgccggaaa
    15061 gcagtaaagt gcgatccgct tcttccggtt ggtaattcag attgagccgc ccgttcgttt
    15121 tggttgcatc tttctcgcca aaccaaccgt tgtaggcaat ttcttttttg ctatccaagt
    15181 tgttgttatt gccggttgta gtaatatctt tattgcctgt aatggtaacg gtggattctt
    15241 tgtcttgatt gtggttgaca atcatcgccc cttcatcggt attttgaatg cggtggaacg
    15301 aaagcgaatg cccgttcaaa tccaaacgtc cgccgcgaaa gccgaaatag agtttgtcgg
    15361 ggttgaactg attatcggca ttcagttgca ccgtccccct gccgctgacc aagccgattt
    15421 cactaaaggc ttgttttttg ccttgatcgt ccgcctgctg atctaagatg actttaccgt
    15481 cgcccacgct gaccgagcct tggttttccc ctttggcttg aaccagcagc gtgcctttgc
    15541 cgattttgga caggcggtcg tttgccacgc cgtttacttt ccaagtaacg gtactgccat
    15601 cactgatatg aacgcccgcg ccttgccacg tttcgttgtt tttaggcgag accgtaaaat
    15661 taccctcaaa atacaaaccg cccgcgcctt ggttgatgtt gctggtaagt atcaattcac
    15721 cttttccttt gtcaataaag gaaatatttt ctccattatt cagtctgggt cgataactgt
    15781 tgaccccacc tgcagcatga taaacaggtt ctcttgctgt ctcggataaa gaaacattaa
    15841 acaattgaac ggttcgtgtt tttaatctat aaggtagaga atagtgttta tgtttggcat
    15901 cgatttttcc tgcgccatta ttattgtcgt taaaaaagta tttcccattt tgatgtggtt
    15961 cgtagaatac tgaatgggta tctccagcaa agatttcatc atagaaccaa tctttacgaa
    16021 ctagctggaa gccattgctt tttcctatat aggggttgcc tgtttgcaat accccattaa
    16081 ttaaccactt ttgcttttgg gcatcataga taaacattgg tgagccactg tcgccaaatg
    16141 agcctcctgt tggtaaaaaa ccatatgggc tatgtttaat tttttcgcta cctaagttga
    16201 ctgtgccacc acctgatcca ttttgtgcaa aggtattgcc accgacgagc caagaatatg
    16261 cgcttgcaat atgatatgaa ctttcgcggt tattgggttc gtcttcatca gaccgccaat
    16321 attgtctgcc tgctccgatt cgaacacgat cagggtattt atttaaatca gcgtatttcc
    16381 acccatccat ataactggtc atctcaacag gttctgcatc tgtgacaaat ttgtgcaaac
    16441 gcggcatatg ataatcgccg ccataaggat ggccgttagt ccctgcttta taattatttc
    16501 ttttcacaat ttggtaagaa aagcggtgct gatcgggatt gcttccctcc gcaccaaaat
    16561 caacattgtt atagccgccg ttatgtgcca cgctcacaat atattgatcg cccgccaatg
    16621 ccgccacgcc gttacgcgat accacagaaa aatcaatcat cggggctttc gtcatcgatt
    16681 tgccgaccaa ctcccctttt ttgttgtaaa cctcaatatc tttcgccccg actgcaaact
    16741 tgcctttatt ttcggcaaag tcgcgatagt attggtagtt gatgccgaaa taagtgtgtc
    16801 ccgcccgggc ttggggcaga atgccgaacg acaggcatat ggctaagtaa gcgggcgaga
    16861 agcggatgcg gccggtttta ggggctttgc ggtgtgtttc ggttgtccgt ttgtcggttg
    16921 ttttcattat ttttccttat ctgacgggat tcggtttgtt tgggagggcg cggcttccgc
    16981 ttccgggcgg cgcgcgggat gtggctatat gtgcggttcg gcgtattttg ccgcttctgt
    17041 tcggagatgg tgcagggaag ggcgggaagg aaaaaaaggg gatttttatc aactcgccgg
    17101 agcaatcgat gggttgcaca taaaggcatc attttgaata cgaaatcata tcacacttta
    17161 ctttatcccc gttccttttc ggtcggaacc ccgccgtttt cggcggtaaa aaccgaccgg
    17221 aggcggaatg cggggcaggg ttttgtgccg tgcggtgtcg gaatatcgtt ttacacaagc
    17281 cggcaatgcc atctgaaagc cgaaccgtcg gacttcagac ggcatttgct ataatcgcgg
    17341 ctgttttgaa ttttcggggg ttttatgtcg gataacgttc caacgattgc ggcagtcgcg
    17401 accgcgcccg gacgcggcgg cgtgggcgtg atacgcatat cggggaaaaa cctgttgccg
    17461 atggcgcagg ctttgtgtgg gaaaacgccc gagccgcgcg tggcaaccta tgctgatttt
    17521 acggacgcgg acggacaggc aatcgatagc gggctgctac tgttttttgc cgcgccggca
    17581 agttttacgg gcgaagatgt catcgagctt cagggacacg gcgggccggt agtgatggag
    17641 atgctgctga accgctgttt ggaactgggc gcgcgccttg ccgagccggg cgagtttacc
    17701 aaacgtgcgt ttttaaacga caaactggac ttggcacagg cggaaggcgt ggcggatttg
    17761 attgacgcat ccggccgttc ggcggcgcgt ctggcgttgc gctcgctcaa gggcgatttt
    17821 tcgcggcgga tacacggttt ggtcgaaggc ttgattacct tgcggatgct ggtcgaagcg
    17881 gcgctggatt ttcccgagga agacatcgat tttctcgaag cggcggacgc acgcggcaaa
    17941 ctggacggct tgcgccgcgc cgtggatgat gtgcttgcca acgcgcagca gggcgcgatt
    18001 ttacgcgaag gtctgaatgt cgtattggtc ggtgcgccga atgtcggcaa gtccagcctg
    18061 ttgaacgcgc tggcgggcga cgaagtggcg attgttaccg atattgccgg aacgacgcgc
    18121 gacgcggtca gggaacgtat cctgattgac ggcgtgccgg tgcatattgt cgatacggca
    18181 ggtttgcgcg agacggacga cgtggtcgaa cgcatcggca tcgaacgcag ccgcaaagcc
    18241 gtatccgaag ccgatgtcgc gctggtgttg gtcgatccgc gcgagggttt gaatgaaaag
    18301 acgcggatga ttttggatac gttgccgtcg gatttgaaac gcattgaaat ccacagcaaa
    18361 tccgatttgc acgcacacgc ggcaggcggg ttcggtacgg gcgcggaaac cgtcatcgcg
    18421 ctgtcggcga aaaccggcga cggcttggac gcgctgaaac ggacgctgtt gtgcgaggcc
    18481 ggttggcagg gcgaaagcga agggctgttt ttggcgcgga cgcggcacgt caacgcactc
    18541 aaggcggcgc aggaagaatt gtcgctggcc gctttgtgcg gcaaccatca aatcgagctg
    18601 tttgccgaac acttgcggct ggctcaggtc gcgtgcggcg aaattacggg ggaatttacg
    18661 gcggacgacc tgctcggcgt gattttttcg aggttttgca tcggaaaata agcgggccgg
    18721 aggcattgtg gcggtgtccg gctgaacatt ccgttatccc ataaaaacgg gaatccgatc
    18781 cgttcggttt tggttttttg aatttcgggc aacgcttaaa tcttcattcc gcgcaggcgg
    18841 aaattatcgg cgcggtacgg caactttttt caatactgaa agccccgtca ttcctgtaaa
    18901 aacaaaaaaa tcaaaaacag aaaactgaaa tatcgtcatt cccgcgcagg cgggaatcca
    18961 gccccgtcgg cacggaaact tatcgggaaa acggtttctt tagattttac gttccggatt
    19021 cccgctttcg cgggaatgac gggattaaag tttcaaaatt tattctaaat aactgaaact
    19081 caacgaactg gattcccgct tttgcgggaa tgacgaattt taggtttctg tttttggttt
    19141 tctgtccttg tgggaatgat gaaattttaa gttttaggaa tttatcggaa aaaacagaaa
    19201 ccgctccgcc gtcattcccg cgtaggcggg aatccagccc cgtcggtacg gaaacttatc
    19261 ggataaaacg gtttctttag attttacgtt ccggattccc gctttcgcgg gaatgatggg
    19321 attaaagttt caaaatttat tttaaataac tgaaactcaa cgaactggat tcccactttc
    19381 gtgggaatga cgaattttag gttgctgttg tttcggataa gttcctgtgg ctttgcgttt
    19441 ctagattccc acttccctgg gaatgacgga gagttgcggg aatgacggag agttgcggga
    19501 ttgggcttgg tacaagcctg aaagggggcg gcggtgcgac cgtaaaaaat gccgtctgaa
    19561 acccaaatca tcgggtttca gacggcattg gcacatcagg agcttagaac ttcgcttcga
    19621 tggtgaaatt gtagctcctg cccggcgagg taaagcgttg gatgccggcg tgggttttgt
    19681 tgtcaacgcg gttgactgtg ccgaattcgc ggatgctgcg taaagattcc caagtgtagt
    19741 actgcttgtt ggtaatgttg tacgcggcgg cgcggagcgt aacctgtttg ccgatgttga
    19801 ggtaggcgga aaggtcgaac agcgtatagg ccttgctgtg tttggcataa ggccacgggt
    19861 tgttcaaatc gtcgttgctg tggacggtgt cggacggctt tttggcggcg gtgcgcgtgg
    19921 cgtaggcgtt gatgccccag cgtttggaag gcgcgtcata gcccaggctg taaaccgccg
    19981 tccacggcga aagcgcgttg atgggcgttt ctttgccgtt gttttgcgtt gccttgccct
    20041 tgatgtagct gacgttgagg ccggtgtgca gccctttggg caaaccgata ctgtcgaggt
    20101 tccacgtgcc gttaaactct atgcctttca cccaggcggc ggagcggttt tggttttgcc
    20161 aaacgggcga gctgaccaat gccgtaccgt ctgaaagcgg ggcgtatctg gggttgtttt
    20221 tatcgtctga cgaaacgccc atatacgtca attcgataaa gtcgcggtat ttggttttga
    20281 agcccgagag cttgaagttg cccgctttgc cgctgcccgc cagacccaat tcccagtttt
    20341 tggctttttc ggctttcagg tttgggttgg ctttcaggta gaaatcgggg tgtgggaaca
    20401 gtagccaagt ttcgtccgaa gtcggtgcgc ggaagccggt gctgtatttt gccaacaagt
    20461 gcagatgctt ggtaaaccgc cagtcgaacc ccgtgccgta gctgaagccc gcgtgcgcgc
    20521 gttccgagcc caaatgggga atctgccccc ggatggcggt ggtgtatttc ggatcgtctt
    20581 tcgcgctgct gctgtttttg tcgtagcgta tgcccgcatt caggcggaag cggtcgttgc
    20641 cgcccaagtg aaacgcattg ttccaatagg caaatttata tttcgaccgg ttttcgatca
    20701 gcattgtgat tttggcttgg ttggaagcga ggattttggg ggcgtacagt tttgcgaaat
    20761 agctgtaatc cgagttggca ttgtcccctc tgccgccgcc caagccgtat tgcgccgccc
    20821 aaacggcttt ggaaaagtcg agttgttttt caaaatcggc agtccattgc gcggtgtttt
    20881 ggcggatatg ccggaacgaa tggtaaacct cgccgttgat gccgcgcaaa tcgtaatttt
    20941 tcgggatgtc ccaagtccaa gtgttcatat cgatgcgctg cttgtcgtag cgcagcttga
    21001 ggctgtccca cgggccgtgt tccaattcgt ttttgtattc gacaccggag cggcggcggt
    21061 agctcacgtc ttggcggtgg cggtagtcgc ccgttgcggc agacgtggtc gtacccgtcc
    21121 acaaattaga cagctcgttg gtaaaacggt cggtgcgcga gtcttcaaaa atccagccga
    21181 tacggttttg atcgttgaag ttgtagccca gcttgaacag ggtgcttttg tttacccatt
    21241 cttgcgggtc gggttgggag cgcgcaaccc ctgtggctac ataggtcagg tacttgctgg
    21301 gtccgcctgt atcggtcggg ttatagacat atccgtcgtt tttaatttcg atatcgccct
    21361 cggtcgagcg gtttttggtt tctttgccga agcggcgggt ataaaccaat aaggcatcca
    21421 aaccaaagag cctgccggcg gcggtgatgc tgctgaattt ttggctgttt ttgccgacgc
    21481 tgccgccctt tatccccaaa tggtagggct tgtcttcgga aacataatcg cttgcggatt
    21541 tggtttggta attgactgcg ccgcccaatg cgccgctgcc ggatttgagc gagtccgcgc
    21601 ctttggtgat ggttacttcg gaaaagtttt ccggctcgga agtgttgcgg ttggcgttga
    21661 agttgccgta cgcgccgaac aattcttgga aggcttcgga agagcggctt tctgcctgcg
    21721 ccagcccgtc aacgttgatg gcgacgcggt ctttgtccac gccgcgtatg gtaaagccgt
    21781 tagaacccgc cctgccgcct tcgacgacgg aaatgccggg gtcgtagcgc accaggtcgt
    21841 gttcgtcgtt gaccaagagc ttgtctaagg ttttgcggcg gattttttct tcgccgagtt
    21901 tttgggtttt gtgcgtgccg gtaacggtga tttcgttcag cgtttgggtg gactgcggcg
    21961 cggggtctgc cgcaaaggcg ggaaacgctt gggcgatggc ggcggcagcc aatacgggtt
    22021 tgaaggggat gtgcataaga aaaccttttt aaaagcggat taattattat tttgtgagat
    22081 gggtggtact catatcagtg ctgttttcaa gttctttttt ataactcaca ccgccgaata
    22141 cggtatcgag ggagcggtcg ccgtcaaagg tgattttgcc gcctatgctg gtttcggtgt
    22201 tgccgtagct gccgttgaat tttccaaaaa acttgccgtc caatttgccg gttttgccgc
    22261 cggacgtggc atcgccggaa aaggtcagac cgctaatcgt tgcttcttta atttccacat
    22321 ccggcccgta atcgctgttg ccgaggattt tgcctgccag tttttgggtg ttgaaattgg
    22381 cggtgataaa ggaaaggacg ggtgtgttgg taggtgtgga cgaataataa ccactgtttt
    22441 tcggcggcgt ataagaggaa gtaacaaatt gcccgtcttt gacgcgaatg ccccaaacct
    22501 gataagtgat tttgcccttt gacaatgcgg tttcgggcgt gctgctgtaa taactatttg
    22561 atatcggcgt tttgccgacg gggaagccgc cggcgaagag gtcgatttcg ccgttggggc
    22621 ctatccacgc cccgaatttc atataatctt gtttggtgat ttccgcatac gaagtagccc
    22681 cggagtcgct gcagcagata aagatgcggc tgccgtcggc gagccgctgc cctgcgtggc
    22741 ggtcgggaat tttgaaatcg ggggaggagt agctgacggt tatggggtcg gcgtaggatg
    22801 aaagggcgta ttgcgcgccg ccgggtgttt tgaagatgaa tgatgtttct tttgatttat
    22861 agccgaaata gtctttttct acttcccgct gccgaatgga aatgccggat gtgttttgcg
    22921 gggcggcatc gagcgagcgt ctgctgcggc tcggcgcgtc ggatgcgctt ccggcgccgg
    22981 cggtgttgat gttttcgata ttgccgttat cgcttgataa cgttacctcg ccggcgggca
    23041 gcggcgtggc ggtggggatg gacacgggga cgtgcggttc ggctaccccc cccccggcac
    23101 aggcggcaag ggcggcggca agcggcagta agggcagggc tttgtatttc atcgcatact
    23161 caatcttgat gatgtattaa taatagtttt gattatcaat ttaaatgtta tatgatgtca
    23221 actgttttat ggggcggcgc ggaaatcgga cgtgcctgcg tttgcctgcc gcgtttctac
    23281 ctttgttttt tatagcggat taacaaaaac cggtacggcg ttgcctcgcc ttgccgtact
    23341 atctgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatatgatg
    23401 cgccgcgtcc gccgcgcgtg gggcttgcgg cgtttctgcc gccgccgtcc ggcggtgttt
    23461 tttaaggaag gcaaattgaa ggatagtgtg tatcgggtat gccggctgtg tgccgcgctg
    23521 tgtgtttggg gtgcggtcgg cgcgtatgcc gccggcttgc ccgacgttcg ggacgatgcg
    23581 gcggcgttgc gcgcccagcg tgcggctgcg gagggttggg cgggaatgcc gccggaaggg
    23641 gattctgccg caaacggcgg ttcgcgcgta atcgacgggg atttcctgct gtcgcgcccg
    23701 cagttgttgg aacacgtttt gcgcgacgcg ctcaacggca atcaggcgga tttgatcgct
    23761 tcgttggcgg atttgtatgc caagctgccg gattatgatg cggtgttgta cggcagggcg
    23821 cgggctttgc tggcgaaatt ggcgggaagg ccggcggagg cggtggcgcg ctatcgggcg
    23881 ttgcacaggg ataatgcggc agacgagcgg gttttgctgg atttggcggc ggcggagttt
    23941 gacgatttcc ggctgaagtc ggcagggctg cattttgcgg aggcggcaaa attggatttg
    24001 ccggcgccgg ttttggaaaa tgtggggcgt ttccggaaaa aaacggaggg gctgacgggc
    24061 tggcattttt cgggcggcat cagcccttct gtgaacaaaa acgccaacaa tgccgcgccg
    24121 caatactgcc tgcagggcgg cggcagccgg atatgcagcg ttaccggacc tgtccgtgcg
    24181 gcagggttgg attatgaggc cggggcggaa aagctgactg cgctggcgga caaccattat
    24241 ctgctgttcc gcgccaatgt cgggggcaca agctattatt tcagccgcaa atcggcttat
    24301 gacgatgctt tcggcagggc ttatttgggc tggcagtata aaaatgcgcg gcagacggtg
    24361 ggggttttgc cgttttatca ggcgcaattg tcgggtagcg acggctttga tgcgaaaacg
    24421 aaaccgcctg ccgaccgccg gcttgcgccg tatatgctgg cgcacggggc gggtgtgcag
    24481 ttggcgcatt cttaccgcct gagccgccgg tcgcagcttt cggtgtcgct ggaacgttac
    24541 cgccaacgct accgcgaaca aggccgggcg gaacgcaacg acggttggca ggacgggctt
    24601 tatgtgtcgc tggcaaggcg gttgggcggc tcggcaaccg tgttcggcgg ctggcagttt
    24661 gcgcggtttg tgccgaaacg cgaaacggtg ggcggcgcgg tcaacaatgc cgcctaccgc
    24721 cgcaacgccg tccatatcgg ttgggcgcag gagtggggcg ggacgggcgg tttgaacagc
    24781 cggattgccg cgtcttacgc ccaccgcaac tacaaaggca ttgcggcatt ttcgacggag
    24841 gcgcagcgta accgcgaatg gaatgtctcg ctggctttga gccacgacaa gttgtcgtac
    24901 aaaggcatcg tgccgacgtt gaactaccgc ttcggtaaga cggaaagcaa tgtgccgtat
    24961 gcaaaacgcc gcaacagcga ggtgtttgtg tcggcggatt ggcggttttg aatggtggga
    25021 taatgccgtc cgaactttgc ggcagcaggt tcggacggca tttttgcgcg ttcaggcaag
    25081 gacggcggca aatacgccgc gcaaggcgtt ggagaggcgg atttcttcgg ctccttgcag
    25141 tgttttttgt gtgatgtgtg tttcaatcac ttgatttgtg tgcaaatatt tttgcggttc
    25201 gtccgacacg gcttggcgca ttatgccgtt taaaatgtct aaatctaaag agggcgtgag
    25261 ccattgtccg cgatgtttga cgaacacgtt gctcctgccg ccttcgagta ggatgccgtc
    25321 cgaattgaaa aacaggctgt cgaacgcgcc ttgcgtttcg gcggtttgcc acgcttggtc
    25381 gaagatggcg cggtgtgtgg ttttgaagcg gcgcaggtag ttttgtgcgg gcaggatggt
    25441 cggcgaaatg atgacgcgct gtttgtcggt cagacggttt aaaacggcgt gggacaggct
    25501 gatgccgtct gaagcgagca gggctttgat gcggaacgcg ccgtcgggca agtcggcaat
    25561 gtattgtttg atttgatttt cgcagccgtc gggcaggggc aggttgaggg cttgggcgga
    25621 ggttttcaga cggcataggt ggcggtcgag cagggcgcat tgcctgtttt ccgcgcgcag
    25681 ggtttcaaaa atgccgaaat cggggcgcag ttcgttgagg aatcgggctt tccagccgca
    25741 ttcgcgatat tcggcggcgg ggtcgctgtc gatgacgatg ccggaaccga caccgtacac
    25801 gccccgatac ggcggattgg cttcaaacgg atgcggcgtt tccccgcctt gtcctgccgt
    25861 tcgggcttgc gcgtttgaat ccgtgccgcc gatgcctgaa acaatgccgt ctgaaacggg
    25921 tttgagcgac agggtgcgga tgacgacgtt gaacgcgcct tcaaacccca agccgccgga
    25981 acacgggttc aaatagccga tgctgcccgt ataaagcccg cgcgcttcgg tttcgagcga
    26041 ttcgataatc tgcatactca tttttttggg cgcgccggtg atgctgccgc aggggaaggc
    26101 ggcgcggagg gtgtcggcga acgaggtgtc cggcagggct tgcgcccgga tggtgctggt
    26161 catctgccag acgctgccga aacgcgatac tttaaacggc tcgggtacgc atactttacc
    26221 ggtttgggcg attttgccga ggtcgttgcg cagcaaatcg acaatcatca cgttttcggc
    26281 gcggtttttc gggtcggttt gtaactcggc ggcgcggcgt tcgtcttgtc cgtcgcccaa
    26341 aatcggcgcg gtgcctttca tcggttcggt gctgatggtg ccgtctgaag cgatgttgag
    26401 gaagagttcg ggcgagaaac acagcgtcca cgcggattgc ccggcttcat cgggcaggtg
    26461 ggacaatacg gcatagggga cgggctggcg caggcggcgg tagaggctga cggggttgcc
    26521 gtaggcttgc aggtgcaggc gggtggtgta gttgatttga taggtgtcgc cgcgccggat
    26581 ggcttcgtgg atttggcgga tgcggtcgag gtaatcggtt tcggatacgg aggattgcgg
    26641 cgtggaaata ccggcgggga tgccgtctga atgtcgggca agccagcttt cggcatcggt
    26701 gtcggcgcag tcggcaaacc agtgcagggc aatgttgccg ccgcgttcgg attccatccc
    26761 catcagcggc aaaccgaatt cgtagtctgc aaacaacacc gaatgcagcc ctttttgcca
    26821 tcccgattgc agcgcgccgt ctaaagcatc gagttcttcg ggacggaaaa aacggctttc
    26881 cacatgattt tgatagagtt ttgcgcgtcg gctgacggca tcgtcaaaca gggcgaaata
    26941 aggcatggca attcggggca aatgttttga ttatacgccc cttttacacg gattttcaga
    27001 cggcggggca aattcgggca acaaaatgta attttatgta gagaaagcgg tggcgaaagt
    27061 gtaaaattgt gaaaacggac tttcatttcc aacctgaaat acaataggag acctttatga
    27121 cagaccgcca gttgcaaccg tttgaaaacg tagaattggg cgaaaagcaa gaccagctcc
    27181 aagtatttga aaaagctgtt ttggaacatg aaggcaaagg ttctgccgaa gattccggca
    27241 cagccccgct gcccgaaaac tacccctgcc gcaaacgtat gcgccgtgcc gtatacgaag
    27301 ccgaaaaagc caaactgcaa atcgagctgc tgaaagtgca aagccgggtc aaagactccg
    27361 gccagcgcat cgtcagcctg tttgaaggcc gggatgcggc aggtaagggc ggcaccatca
    27421 aacgctttat ggaacattta aacccgcgtg gcgcgcgcgt ggttgctttg gagaaaccaa
    27481 ccactaccga acgcggacaa tggtatttcc aacgctacat ccaaaacctg ccgactgcgg
    27541 gcgaaatggt attcttcgac cgctcgtggt acaaccgcgc cggcgtggaa cgcgtgacgg
    27601 gcttctgcga acccaacgaa tatatgctct ttatgtgcca agcccctgag ttggaacgta
    27661 tgcttgtcgc cagcggcatc cacctgttca aattctggtt ctccgtatcc cgcgaagaac
    27721 aactgcgccg cttcatctcc cgccgcgacg acgccctgaa acactggaaa ctttcccccg
    27781 tggacatcca gtcgctcgac cgctgggacg actacaccga agccaaaaac gcgatgttct
    27841 tccacaccca caccggcgac gcgccttggg tcatcatccg ttcggacgac aaaaaacgcg
    27901 cccgtttgaa ctgtatccgc tacttcctgc accagttgga ctacccgggt aaagacgtga
    27961 aagccatcgg caaagtggac gataaaatcg ttcttgttcc cgatacgcgt tacaaagaga
    28021 aaaccatcga tgtcggtcat gactgattgc cggtgaagta ataaaaatgc cgtctgaacc
    28081 cgatttcagg ttcagacggc attttctatc cgtgtgtcaa accggcatca gatgtcggtt
    28141 tccagataaa tgacttgggt ttgcaggtat tcttccaaac cgtgtttgcc gtccgcgccg
    28201 ccgatgccgg attttttcca gcccgcgtgg aagccctgca tggcttcgaa gttttcgcgg
    28261 ttgatgtagg tttcgccgaa ttgcaggcgg cgggtaacgt agaaggcttc gttcaggttg
    28321 gtggtgtaca cggagctggt cagaccgaat tcgcagtcgt ttgccagggc gatgacctgg
    28381 tcgagcgtgt cgaaagcgga aacgggcagc acgaggccga aagtttcttc tttcataatg
    28441 tccatactgt tgtcggtgtc ggtcagcagg gtaggctcga agaaatagcc gcgtccttcg
    28501 gtgcgtttgc cgccgcaaac caatttcgca ccttgtttga cggcccgttc cactttttcg
    28561 gcaacggctt tgacggcgcg ctcttcaatc agcgtgccca tttccggcgc gcctgcttcg
    28621 gcttcggcag ggttgccgta gcgcacgcct ttcatcgcgg cggtcatttt ttcaatgaac
    28681 gcgtctttca ggctgctgtg gacatagacg cgctcggcgc agttgcagat ttgaccggtg
    28741 ttgccgacgc gcgaagccaa gatggatttc accgctaaat ccaaatccgc gtctttcaac
    28801 acgatggcgg gggctttgcc gccgagttcc agcgagactt tggtgatgtt ggcggaagcg
    28861 gcttccatca cttggcgacc cgcttcaacc gagccggtca ggctgaccat atcgacttgc
    28921 ggatgggcgg acaaggcgtt gccgatttcc gcacccggac cgtccaccac gttgaacacg
    28981 cctgcgggca gtccgaccgc atcgatgatt tcggcgaaga tgtggcagtt gatcggggtt
    29041 acgctgctgg gtttgacgac gatggtgttg cccgtaacca gatccgggcc cattttgcgg
    29101 gcgatcagga agaaggggaa gttccgcggc aaaatgccgg caatcacgcc cagcggacgt
    29161 ttgaacgata aaatgttttc gcgcgggcgg tcgctttgga tgatttcgcc ttcgtaacgg
    29221 cgcgcccatt cggcttggta atcgagatag tcggcagtga acatcacttc cacacgcgcc
    29281 aagtctttgg ttttgccgcc ttcggcaacg atggtgtcgg tcagctcgtc ggcgcgtccg
    29341 cgtatgcctt gggcgatttt acgcaaatac gcgccgcgtt cgaccgcagg cagacgctcc
    29401 caagccggtt gcgccgcgcg cgccgcggtc aacgtccgcc ctgccgcctt tgggttcgcg
    29461 ggcgatggtt tcttcggtgg acgggttcaa tacgtcgcgc cattcgccgt tgaaatcgtt
    29521 ttcaaagcgt ccgttgatgt acatggccaa ttgtttcatt tcgggccctc cagttttgta
    29581 gtcggatgta gttttggttt attcccaaat aaattgccgt acaagtttct ttacacgcgg
    29641 attttgggtt tcaagtcaaa tacggcttac cggttttctt ttttagtttc cgcaggcgtg
    29701 ctgccgaaat attgcctgaa tgcctgaata aagctggaaa cgtggcgcta gccgcacaaa
    29761 tacgcggttt cgcccacgtt tttgcccccg ttttgcaaca gatagagcgc gtgctgcatt
    29821 tgtttgtggt gcagccactc gcttgccgta atgccgaaat ggtcgcgcat acggcgttgc
    29881 agcgtccttt cgctgatgtt cagcgcggct gtcagccggt tgacttggtg tgcgccgccg
    29941 tcgaacgcgg cattcagggt gcggctgaag tcttcagacg gcatagcgtc tgcttccgcc
    30001 gtttgccccg ccgccggctc gatgccgtct gaaaccgtgt cccacaaatc cgacagcagc
    30061 cgcaacacgt ccgcctcgcg gcgcaatgtt tcgcccaaat gcccctttgg gacggtttgc
    30121 aggcaggatg ccgccaagcc gcgcaggttt gggggcaaat cccatatcct gaccggttcg
    30181 cggtaaagca ggggtgcgaa acgcgcgtat tccggacgcg gcagccattg ttccatacct
    30241 ttaatggtca ttttgaccgt tttgccgccg cgataaagat agcggctgaa caggacttct
    30301 tccccgacgg caattaggac aatcttgccg ccgtccgcat cgatgcggaa gcggcggcgg
    30361 ttgatgccga agtccaaacg cccttccagc aacagcacga acgacacata aggttcggcg
    30421 aggcggctgc tgcaaaaatc gcaccgtgcc gttaccgtac cgccgtggat ggaaatgccg
    30481 ttggacaaag tatcgaaacg gtagctgccc tccacataag ccgccgccgc ttttgaccag
    30541 attgacgaac cggctgctgt tcaaatgttc tgctttttcc atagttttct gataaaggtt
    30601 atcatttgaa agataacatt tttcgccaca gcaaacaatc cgccgccgac aaaggcattt
    30661 tcacaacact tgccgtccgt atgccgtctg aaaattgccc gtcttcccga taagccttaa
    30721 aatacacgcc gtcaaaccta ccgtccgccc gtacatgagc ccatccccct ttatcgaaat
    30781 gaaagacgtc gccttcgcgt atggcgaccg cccgattctg aacgacatca atttcagcat
    30841 tccgcaaggc aattttgccg ccgtgatggg cggttcgggc agcggcaaaa ccacgctgat
    30901 gaggctgatt acaggacaga ttcgtccgca gtccgggcag gttttgattg aaggacggga
    30961 tttggcgggt ttttcggctg acgaactcta cgaacaccgc cgccgtatgg gcgtattgtt
    31021 ccaacacggt gcgctgttta ccgatttgtc ggtattcgac aatatcgctt ttccgatgcg
    31081 cgaactgacg caacttccgg aagcggttat tcgcgatttg gttttgttga aattgaacgc
    31141 ggtcggtctg cgcggcgtgg aaaacctgat gccgtccgag ttgtccggcg ggatgtcgcg
    31201 ccgcgtcgcg ctggcacgca caatcgcgct cgaccccgaa attatgttgt acgacgaacc
    31261 gtttaccggc ctcgacccga tttccttggg cgtgattgcc cacttgatca gccgcgtcaa
    31321 caaggccttg cgttcgacca gcattatggt aacgcacgac attgaaaaat ctttggaaat
    31381 cgtcgatcag gtaattttct tggcgcacgg cgaaattatg ttctccggct cgccgcagga
    31441 aatgcgcgaa ctggattcgc cttgggtgcg ccagtttgtc ggcgggctgg cagacggccc
    31501 cgtggcatac cgctatccgg cgcaaacgtc gttgcagcag gatttgctcg ggtaaatgag
    31561 caggtgccgt ctgaaaccgt tgaaccttac atatagaaat ttatgaattt tatccgttcc
    31621 gtcggggcga aaaccctcgg ccttattcaa cccttcggca gtatcacgct gtttctgctg
    31681 aacattttgg cgaaatccgg cacggctttc gcccgtccgc gcctgagcgt gcgccaagtg
    31741 tattttgccg gcgtgctgtc ggtgctgatt gttgccgttt cggggctgtt cgtcggtatg
    31801 gttttgggtt tgcagggcta tacgcagttg tcgaaattca aatccgccga tattttgggc
    31861 tatatggtcg cggcttctct gttgcgcgaa ctgggtcccg tgttggcggc gattctgttt
    31921 gccagcagcg cgggcggtgc gatgaccagc gaaatcggtt tgatgaaaac gaccggacag
    31981 ctcgaagcga tgaacgtgat ggcggtcaac cccgtcgccc gcgtggttgc cccgcgtttt
    32041 tgggcgggcg tgttttctat gccgcttttg gcttcgattt tcaacgtcgc gggcattttc
    32101 ggcgcgtatt tggtcggcgt gagctggctg ggtttggaca gcggtatttt ctggccgcag
    32161 atgcagaaca acattacgat acattacgat gtaatcaacg gtttgatcaa atccgccgcg
    32221 ttcggcgtgg cggtaacgct gattgccgtg catcagggct tccactgcat cccgacttcg
    32281 gaaggcattt tgcgcgccag cacgcgcacg gtggtttcgt ccgccctgac gattttggcg
    32341 gtcgatttta tattgaccgc gtggatgttt acagattgac agactgtctg aagacgaaac
    32401 gcggaacatc ggatattcaa ggaactttaa tgaaaaagaa catattggaa ttttgggtcg
    32461 gactgttcgt cctgatcggc gcggcggcgg ttgcctttct cgctttccgc gtggcgggcg
    32521 gcgcggcgtt cggcggttcg gacaaaactt acgccgttta tgccgatttc ggcgacatcg
    32581 gcggtttgaa ggtcaatgcc cccgtcaaat ccgcaggcgt attggtcggg cgcgtcggcg
    32641 ctatcgggct tgacccgaaa tcctatcagg cgagggtgcg ccttgatttg gacggcaagt
    32701 atcagttcag cagcgacgtt tccgcgcaaa tcctgacttc gggacttttg ggcgaacagt
    32761 acatcgggct gcagcagggc ggcgatacgg aaaaccttgc tgccggcgac accatctccg
    32821 taaccagttc tgcaatggtt ctggaaaacc tgatcggtaa attcatgacc agcttcgccg
    32881 agaaaaacgc tgagggcggc aatgcggaaa aagccgcaga ataaacatac acatccattt
    32941 tcaggaaaga catcatgaaa aaatcctcct tcatcagcgc attgggcatc ggtattttga
    33001 gcatcggcat ggcatttgcc tccccggccg acgcagtggg acaaatccgc caaaacgcca
    33061 cacaggtttt gaccatcctc aaaagcggcg acgcggcttc tgcacgccca aaagccgaag
    33121 cctatgcggt tccctatttc gatttccaac gtatgaccgc attggcggtc ggcaaccctt
    33181 ggcgtaccgc gtccgacgcg caaaaacaag cgttggccaa agaatttcaa accctgctga
    33241 tccgcaccta ttccggcacg atgctgaaat tcaaaaacgc gaccgtcaac gtcaaagaca
    33301 atcccatcgt caataagggc ggcaaggaaa tcgtcgtccg tgccgaagtc ggcatccccg
    33361 gtcagaagcc cgtcaatatg gactttacca cctaccaaag cggcggcaaa taccgtacct
    33421 acaacgtcgc catcgaaggc gcgagcctgg ttaccgtgta ccgcaaccaa ttcggcgaaa
    33481 tcatcaaagc caaaggcatc gacgggctga ttgccgagtt gaaagccaaa aacggcggca
    33541 aataaccgac aaaccatacc gtccgagggc ggtatgggtt tttcgaacaa acgctatgca
    33601 tacagaactc aaaaacggaa cactgcacat cggcggcgac atcaccgtca aaaccctgac
    33661 cggggacgca tttgggcgtt tccggcaaca atgccgtctg aaagaaacca ttgccgtcga
    33721 tttcggaggt gtcaaacgtg ccgattccgc ctgcgtgtcg ctgctgctcg aagtgctgcg
    33781 cggctgcaaa ggcagcgtca ggctgaccgg cattcccgaa tccgtgcgcg cgctgtccga
    33841 actgtacgaa atcaaagact ggctgaaatc atgaaaaaaa ccgcctacgc catcctcctg
    33901 ctgatcgggt tcgcttccgc ccctgcattt gcagaaaccc gccccgccga cccttatgaa
    33961 ggctacaacc gcgccgtttc caaattcaac gaccaagccg accgctacat tttcgcccct
    34021 gccgcgcgcg gctaccgcaa agttacgccg aaacccgtcc gcgccggcgt gtccaatttt
    34081 tttaacaacc tgcgcgacgt ggtcagtttc ggcagcaata tcttgcgttt ggacatcaaa
    34141 cgcgcaagcg aagacctcgt ccgcgtcggc atcaatacca ccttcggttt gggcgggctc
    34201 attgatattg ccggcgcggg cggcgttccc gacaataaaa acactttggg cgacacgttt
    34261 gcctcgtggg gctggaaaaa cagcaattat ttcgtgttgc ccgtcttagg cccgtccacc
    34321 gtccgcgacg cgctcggcac gggcattacc tctgtttatc cgcccaagaa tatcgttttc
    34381 catacccctg ccggacgctg gggcacgact gccgctgccg ccgtcagtac gcgcgaaggc
    34441 ctgctcgatt tgaccgacag tctggacgaa gccgccatcg acaaatacag ctacacgcgc
    34501 gacctctata tgaaagtccg cgcacggcag accggtgcaa cacctgccga aggtacggaa
    34561 gataacatcg acatcgacga attggtcgaa agtgccgaaa ccggcgcggc agagcccgcc
    34621 gttcacgaag attccgtatc cgaaacacag gcagaagcag caggggaagc cgaaacgcaa
    34681 cctggaacac aaccctaaag ccatgccgtc tgaaaatccg tgttcggacg gcattccctt
    34741 ccgtcggaac ggacagtccg acactacaga ttgggctata atcccttttt tgcgcatacc
    34801 gcgcgacagc aaaggaaaaa ccatgtacga agtcaaccgc agcgtattcg tcctcatccc
    34861 tttagaacct ttttggaact ggctgcaaac cctgccgggc aaccacctcg acggactgac
    34921 cctcgaagac atccaagccg atgccaattc ctaccttgtc cgcccgtgcg aaaccgccga
    34981 cgaagtatgg gacgaaatcg aagcccgttt cgaagacatt ttcgccgccg aacttgccga
    35041 ctggtgcgaa gacgaacgcg agtggcccgc actcgatgcc gacattttca acgaatggtt
    35101 cgacatccag ctttctaccg tcatcaccga cctcgaacac gaaccgcttg cccgcgaagc
    35161 cttccaaccc atcaacctga actgatgaag ctgaccgtcc gcaattacca tctcgacggc
    35221 tacggtcatg tcaacaatgc acgctacctc gaatttctcg aggaagcgcg ctgggcgttt
    35281 tttgaaaaac gcggactgat gcacgagttg gcaggcctca tactgattgt cgcccgcatc
    35341 gacatccgat acagccgtcc cgccgtcgaa ggcgacgtat tgcagttttc atgccgcctg
    35401 aaaacccccg gcatgcgccg cattgtgctg acccagacga ttacactgcc aaacggcaaa
    35461 accgccgcag aagccgacat caccctgatg cccgtccacg ccgccacaca acgcaccgtc
    35521 agcctgcccg ccacccttgc ccgcgcactg gaagccttgt ccgaatgaaa aaaatactca
    35581 ccgccgccgc cgtcgcactg atcggcatcc tccttgccac cgtcctcatc cccgacagta
    35641 aaaccgcgcc cgccttctcc ctgcccgacc tgcacggaaa aaccgtttcc aacgccgacc
    35701 tgcaaggcaa agtcaccctg attaattttt ggtttccctc ctgtccgggt tgtgtgagcg
    35761 aaatgcccaa agtcatcaaa acggcaaacg actacaaaaa taaagatttc caagtcctcg
    35821 ccgttgccca gcccatcgat ccgatagaaa gcgtccgcca atacgtcaaa gactacggac
    35881 tgccgtttac cgtcatttat gatgcggaca aagccgtcgg acaggcattc ggcacacagg
    35941 tttatccgac ttccgtcctt atcggcaaaa aaggcgaaat tctcaaaacc tacgtcggag
    36001 aacccgattt cggcaaactc taccaagaaa tcgataccgc gctggcgcaa tagcgatgcc
    36061 gtctgaagcg caaaaaacag aaacgatgtt cctgtttttt cagatcatcc gccccgacac
    36121 gttcaggcgg cattcaacct tccggcagat tcttttttac caccgcagga ttgcctgccg
    36181 ccaaagagta gggcggaatg tcttttgtaa ttaccgcgcc tgcgcccacg accgagccgc
    36241 gtccgacggt tacgcccgcc atcacaatca ccctgcgccc cggccagaca tcgtcctcca
    36301 acgtaatcgg acggatttcc gtgtagccct caaagcgttt ttttcacggt caaacttgtg
    36361 gttggtcgaa tagaacaggc attccggccc catcatcaca tttttgccga ccaccagtcc
    36421 acggcagatt tcacagtttg ccccgatgcc cgagccgtcg cccaaaaccg tatccggaaa
    36481 cacatacgcc ccgcgtccga tatgcggcga aatccgccgc gccagaaaac cgagcacccg
    36541 tctgccgatg atgccgatgc cgcgcatatg cgacggcggc agcaccgaac cggcaaaacg
    36601 cagcagcagg aaaaagaatt ttgattccat caaaccttcc tcaaaagaac tttttacatt
    36661 ttatcgaata tcctcttgac gaaacaacag aaaatagcga gaatgccgag cttgtctgag
    36721 gcataggtac atcccctatg cccgcttttt tgaaaggaaa tcaaatgaaa caaggtattc
    36781 acccgaacta ccgcgaagtt aacgttacct gctcttgcgg caacaaattc gtaaccaaat
    36841 ccgcaatgga aaaagaaaac ttcaacatcg aggtttgctc cctgtgccac ccgttctata
    36901 ccggcaccca aaaaatcgtc gataccaccg gccgcgtgga caaattcaac aacaaattcg
    36961 gcaacctgtt caaacgctga ttgccgcttt gcaagaatta agccctgcca tcatttcgat
    37021 ggcagggctt tttaatattt ggcacactga cttcttatct atatagtgga ttaacaaaaa
    37081 tcaggacaag gcgacgaagc cgcagacagt acaaatagta cggcaaggcg aggcaacgcc
    37141 gtaccggttt ttgttaatcc actatatttt gacggggttg aagttgacag ctacaaagtt
    37201 taatgtttga atacattaaa aaatatattt gaatgggatg ttttatgatt caaaatgttg
    37261 ttacttcaat aatcctgtat tctgggacag ccgtagactt acttattatc ctaatgttat
    37321 tttttgccaa aaaaaaagca gaaaagacat cattaacatc tatttaggac aatttctagg
    37381 ctctgttagc ctgatattgc taagtttgct ttttgcattt gtcttagatt atattcctag
    37441 taaagagatt ttaggtttgc tcggcttgat tccgattttc ctaggcctca aagttttgct
    37501 gttaggagat tccgatggag agtctattgc caaagagggt ttgcgcaaag ataataaaaa
    37561 cctgattttt ctagtcgcta tgattacttt tgcaagttgt ggcgctgaca atattggtgt
    37621 ctttgtccca tattttacta ccttaaattt agcgaatttg atagtggctt tacttacctt
    37681 tctagttatg atttatctct tggttttttc tgcccaaaaa ttggcacaag tcccttctgt
    37741 cggagaaact ttggaaaaat atagcagatg gtttgttgcc gttgtttatt taggattggg
    37801 gatatatatc ctgattgaaa acaacagttt tgatatgcta tggactgtgt cgggccagga
    37861 aaaaatatta tgaaaaagat agtatctgca aagactgccc cgtgcagtct tttttgttgc
    37921 cggtcttttt gtgtctgatg ccgtctgaag cagtctctgc acgacctttg tgcgaatatt
    37981 tgctacactt ggcaatccgt ccatttcttc tcac
//
LOCUS       DBJGGI010000013        35080 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.013, whole genome shotgun sequence.
ACCESSION   DBJGGI010000013 DBJGGI010000000
VERSION     DBJGGI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35080
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.013"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            58..453
                     /locus_tag="ACSIGJ_000657"
                     /pseudo
     CDS             58..453
                     /locus_tag="ACSIGJ_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(827..1717)
                     /gene="sucD"
                     /locus_tag="ACSIGJ_000658"
     CDS             complement(827..1717)
                     /gene="sucD"
                     /locus_tag="ACSIGJ_000658"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HHQ1329602.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLDLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(1728..2894)
                     /gene="sucC"
                     /locus_tag="ACSIGJ_000659"
     CDS             complement(1728..2894)
                     /gene="sucC"
                     /locus_tag="ACSIGJ_000659"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HHQ1329603.1"
                     /translation="MNLHEYQAKELLASYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREKAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFAKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVNVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            complement(2966..3253)
                     /locus_tag="ACSIGJ_000660"
     CDS             complement(2966..3253)
                     /locus_tag="ACSIGJ_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329604.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAVMYLLARMWLPFSK"
     gene            complement(3373..4806)
                     /gene="lpdA"
                     /locus_tag="ACSIGJ_000661"
     CDS             complement(3373..4806)
                     /gene="lpdA"
                     /locus_tag="ACSIGJ_000661"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HHQ1329605.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGAKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            complement(5067..6248)
                     /gene="odhB"
                     /locus_tag="ACSIGJ_000662"
     CDS             complement(5067..6248)
                     /gene="odhB"
                     /locus_tag="ACSIGJ_000662"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HHQ1329606.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATVAAEAPAAAPAEAAPAAVPAA
                     AQNNAAMPAAAKLAAETGVDVNVLQGSGRDGRVLKEDVQNAAAKPAAAVAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVTALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPVRLLLD
                     L"
     gene            complement(6348..9176)
                     /locus_tag="ACSIGJ_000663"
     CDS             complement(6348..9176)
                     /locus_tag="ACSIGJ_000663"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HHQ1329607.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLEKPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALRFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQVEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREKIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLLTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRNMGEGLGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(9381..10664)
                     /gene="gltA"
                     /locus_tag="ACSIGJ_000664"
     CDS             complement(9381..10664)
                     /gene="gltA"
                     /locus_tag="ACSIGJ_000664"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HHQ1329608.1"
                     /translation="MSKSIKLNVPGRAGLELPVLEASIGHDVVDIRGLTKNTGLFAFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDITNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPAGER"
     gene            complement(10776..11024)
                     /locus_tag="ACSIGJ_000665"
     CDS             complement(10776..11024)
                     /locus_tag="ACSIGJ_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HHQ1329609.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALIHGHSETDKGHLIPMLEKIRRA"
     gene            complement(11028..11735)
                     /locus_tag="ACSIGJ_000666"
     CDS             complement(11028..11735)
                     /locus_tag="ACSIGJ_000666"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HHQ1329610.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRSLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRVV"
     gene            complement(11855..13618)
                     /gene="sdhA"
                     /locus_tag="ACSIGJ_000667"
     CDS             complement(11855..13618)
                     /gene="sdhA"
                     /locus_tag="ACSIGJ_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HHQ1329611.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVQEIMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(13621..13962)
                     /gene="sdhD"
                     /locus_tag="ACSIGJ_000668"
     CDS             complement(13621..13962)
                     /gene="sdhD"
                     /locus_tag="ACSIGJ_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HHQ1329612.1"
                     /translation="MVERKLTGAHYGLRDWVMQRATAVIMLIYTVALLVVLFALPKEY
                     PAWQAFFSQAWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSVKVIWG"
     gene            complement(13956..14333)
                     /gene="sdhC"
                     /locus_tag="ACSIGJ_000669"
     CDS             complement(13956..14333)
                     /gene="sdhC"
                     /locus_tag="ACSIGJ_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HHQ1329613.1"
                     /translation="MSVKLRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGILWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(14620..16023)
                     /locus_tag="ACSIGJ_000670"
     CDS             complement(14620..16023)
                     /locus_tag="ACSIGJ_000670"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HHQ1329614.1"
                     /translation="MKKIQADIVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMRRVKSERDRFVGFVV
                     TDVEEWPADKRIMGSAKFIDEHTVQIDDHIQIAAKSFVIATGSRPVILPQWQSLGDRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIDKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGSGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(16272..17009)
                     /locus_tag="ACSIGJ_000671"
     CDS             complement(16272..17009)
                     /locus_tag="ACSIGJ_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HHQ1329615.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAICCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKVKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(17288..19564)
                     /gene="metE"
                     /locus_tag="ACSIGJ_000672"
     CDS             complement(17288..19564)
                     /gene="metE"
                     /locus_tag="ACSIGJ_000672"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HHQ1329616.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLDVAKDLRE
                     KNWKHQAAANADYVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLPLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQTLGLKAKPTVVGPL
                     TFLWVGKEKGSVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLNKVSAKILLGTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFAGY
                     DKVLSAGVIDGRNIWRANLNKVLETVGPLQAKLGERLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPVNADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSASDYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQNLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRATVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLAAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(19700..20578)
                     /gene="metF"
                     /locus_tag="ACSIGJ_000673"
     CDS             complement(19700..20578)
                     /gene="metF"
                     /locus_tag="ACSIGJ_000673"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HHQ1329617.1"
                     /translation="MNYAKEINALNNSLSDLKGNINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASHDELRQIAK
                     DYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPKWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGARP"
     gene            20775..21050
                     /locus_tag="ACSIGJ_000674"
     CDS             20775..21050
                     /locus_tag="ACSIGJ_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HHQ1329618.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAGTHGKTMVWTDGKE
                     YPLFSLDTSSSSHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            21050..21175
                     /gene="ykgO"
                     /locus_tag="ACSIGJ_000675"
     CDS             21050..21175
                     /gene="ykgO"
                     /locus_tag="ACSIGJ_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HHQ1329619.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKSRQR"
     gene            complement(21261..21479)
                     /locus_tag="ACSIGJ_000676"
     CDS             complement(21261..21479)
                     /locus_tag="ACSIGJ_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MIGRI"
                     /protein_id="HHQ1329620.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRVLREVAEISAWVLLG
                     AAAATLFWYLFMLYFKHIPDSYRRKNAV"
     gene            21622..22761
                     /gene="metX"
                     /locus_tag="ACSIGJ_000677"
     CDS             21622..22761
                     /gene="metX"
                     /locus_tag="ACSIGJ_000677"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase MetX"
                     /protein_id="HHQ1329621.1"
                     /translation="MSQNTSVGIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPMVTVKDWVKSQAALADYLGIEQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYSVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVTAYMNNVDKDCRL"
     gene            22758..23339
                     /gene="metW"
                     /locus_tag="ACSIGJ_000678"
     CDS             22758..23339
                     /gene="metW"
                     /locus_tag="ACSIGJ_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HHQ1329622.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALAEHKKCSGYG
                     IEIDTDNVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RLAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            23456..24274
                     /locus_tag="ACSIGJ_000679"
                     /pseudo
     CDS             23456..24274
                     /locus_tag="ACSIGJ_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230442.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NMB0938 family lipoprotein"
     gene            complement(24434..24994)
                     /gene="efp"
                     /locus_tag="ACSIGJ_000680"
     CDS             complement(24434..24994)
                     /gene="efp"
                     /locus_tag="ACSIGJ_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HHQ1329623.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVDYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(25037..26188)
                     /gene="earP"
                     /locus_tag="ACSIGJ_000681"
     CDS             complement(25037..26188)
                     /gene="earP"
                     /locus_tag="ACSIGJ_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HHQ1329624.1"
                     /translation="MNTYAFPVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWT
                     DDVSALRALCPDLPDVPFVHQDIHVRTWHSDAADIDTAPVPDAVIETFACDLPENVLN
                     IIRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYRE
                     AVRFDTEALRRRLVLPEKNAPEWLLFGYRGDVWAKWLDMWQQAGSLMTLLLAGAQIID
                     SLKQSGVIPQNALQNDGGVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVR
                     TQLAGKPFFWHIYPQDENVHLDKLHAFWDKAYGFYTPETASVHRLLSDDLNGGEALSA
                     TQRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(26207..26485)
                     /locus_tag="ACSIGJ_000682"
     CDS             complement(26207..26485)
                     /locus_tag="ACSIGJ_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329625.1"
                     /translation="MSRNLLVRWLAVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFETIKHHLKQGFDLKRQTMFLFIPIVLLVVYLFHYFGAF"
     gene            26836..27777
                     /gene="miaA"
                     /locus_tag="ACSIGJ_000683"
     CDS             26836..27777
                     /gene="miaA"
                     /locus_tag="ACSIGJ_000683"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HHQ1329626.1"
                     /translation="MPTPKAFTLLGPTACGKTALALKIAETLPVEIISLDSALLYTGM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLTGEITARGKCPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEVYYLTGRPMSAHLNGQPEHTLPFELYTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEHLDGATDRQTFIEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSGTRLIEAAKRFFGE"
     gene            27781..28500
                     /gene="tadA"
                     /locus_tag="ACSIGJ_000684"
     CDS             27781..28500
                     /gene="tadA"
                     /locus_tag="ACSIGJ_000684"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HHQ1329627.1"
                     /translation="MLATPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTPPQEMSQAHKDCLLAELKNHPPVAAFPPQEEMGYFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIITSAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            28505..28993
                     /locus_tag="ACSIGJ_000685"
     CDS             28505..28993
                     /locus_tag="ACSIGJ_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HHQ1329628.1"
                     /translation="MTALLVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGILWTVGLISLGGILADYMAGMLGVKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILLILLVKYIAY
                     LF"
     gene            29059..29811
                     /gene="rlmB"
                     /locus_tag="ACSIGJ_000686"
     CDS             29059..29811
                     /gene="rlmB"
                     /locus_tag="ACSIGJ_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HHQ1329629.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSITELYIQEGKSDARTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDIGGNADLYHCNLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNKKA"
     gene            complement(29873..31247)
                     /locus_tag="ACSIGJ_000687"
                     /pseudo
     CDS             complement(29873..31247)
                     /locus_tag="ACSIGJ_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688214.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
     gene            31627..32502
                     /gene="dapA"
                     /locus_tag="ACSIGJ_000688"
     CDS             31627..32502
                     /gene="dapA"
                     /locus_tag="ACSIGJ_000688"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HHQ1329630.1"
                     /translation="MLQGSLVALITPMNQDGSIHYDQLRQLIDWHIENGTDGIVAAGT
                     TGESATLSVEEHLSVIEETVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAVPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNVGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGNIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCGPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            32511..33707
                     /gene="bamC"
                     /locus_tag="ACSIGJ_000689"
     CDS             32511..33707
                     /gene="bamC"
                     /locus_tag="ACSIGJ_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HHQ1329631.1"
                     /translation="MPSEPFGRHNMTNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIESEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGDKNKDTTMWQPSASDPNLEAAFLTRFMQYLGVDGRQAENALAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTGLALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVADGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(33764..34696)
                     /gene="pip"
                     /locus_tag="ACSIGJ_000690"
     CDS             complement(33764..34696)
                     /gene="pip"
                     /locus_tag="ACSIGJ_000690"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HHQ1329632.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFHPDVFRIVIIDQRGCGRSHPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEGVDEDAYASL
                     AIARLENHYFVNGGWLQGDKAILNNIGKIRHIPTVIVQGRYDLCTPMQSAWELSKAFP
                     EAELRVVQAGHCAFDPPLADALVQAVEDILPRLL"
BASE COUNT         8682 a         9483 c         8692 g         8223 t
ORIGIN      
        1 cactatatat agaaggtata tatcctagta ggcgtaatgg cagctattac aaaatgtctg
       61 catcaggttc acgaaacgca agtcgaaagc gtcaaacaac tgattgcccg ttttgaccgg
      121 ctgattgacg aatcggacaa acaaatcgac aaccacaccc gcacgcattt tgacggcaaa
      181 gcccaagtgg cggagcaaat caaaggcatc ggttcgataa cgacggctac gctgatggcg
      241 atgctgcccg aattggggcg gctgtcgcac aaacggatag cgggtttggt cggcattgcc
      301 ccgcacccga gggagagcgg ggaaaccaaa ttcaaaagcc gctgcttcgg cggaaggtct
      361 gcggtgcgta aggcgctgta tatggctgcc gcggcagcga cacgttttga accgcttatt
      421 cgggatttcc accaacgcct gctgtatagc ggattaacaa aaaccggtac ggcgttgccc
      481 cgccccggct caaagggaac gattccctaa ggtgatgaag caccgggcgg atcggttccg
      541 taccatttgt accgtctgcg gcccgccgcc ttgtcctgat ttttgttaat ccgctatggt
      601 ttaaagcttt gtttcttaag tccgcagagt atgccatggt tagaccttca acgttgagtg
      661 ttgtactatt ttgtttttaa tccactatat tctgactgcc tcaaagggca aaaataatgt
      721 gagtatgttg aggctgcctc tttttgaaat gacaaacaaa atgccgtctg aaacccgatt
      781 tccggtttca gacggcattt gatgttcaat cagttgtcaa acctgattat gccaaacctt
      841 ttgctttcaa cacttccagc atcgtcgtgc ccaactcggc agggctgcgg gtgtaggcga
      901 taccggcttt ttcaaacgcg gcgaattttt cttcggcagt acctttaccg ccggagataa
      961 tcgcaccggc gtggcccatg cgtttgcctt taggagcggt aacaccggcg atgtaaccga
     1021 caacaggttt ggttacgttg gattggatgt attcggctgc ttcttcttcc gcagtaccac
     1081 cgatttcacc aatcatgatg atggcatcgg tatctgggtc ttcttggaag agtttcagtg
     1141 cgtcgatttg gttcatacca ggaatcgggt cgccgccgat accgatacag gttgattgac
     1201 ccaagcccag tttggtggtt tgtgccacgg cttcgtaagt caatgtaccg gaacgggaaa
     1261 taataccgat gcggcccgga gtgtggatgt ggcccggcat aatgccgatt ttgcactcgc
     1321 ccggagtaat cacgcccggg cagttagggc cgaccaaacg ggtaccgttg ccgttggttt
     1381 ccaagtaacg tttggctttg agcatgtcca gagttggaac gccttcggta atcacaacga
     1441 ccaagcctac gcctgaatca acggcttcaa cgatagaatc caacacaaac ggagcgggaa
     1501 cgtaaatcac ggatgcgtca gcgccggttt ctttaacggc ttctttcatg gtgttgaata
     1561 caggcaggtc taggtgggtt tgaccgcctt tgcctggggt aacgccgccg acaactttag
     1621 tgccgtaagc cagagcttgt tcggagtgga aagtaccgtt tttaccggtg aaaccttgaa
     1681 ccaatacttt ggtgtcttta ttaatcaata cgctcattct tttctcctta ggcgtttacg
     1741 gctgcaacaa ttttttcggc tgcgtcattc agaccgtctg cagaagtcag tttcagacct
     1801 gattcgttca ggattttcgc gccgagttcg gcgttgttgc cttccaaacg aacaacgaca
     1861 ggaacgttga cgttgatttc tttaacggct gccacgatgg cttccgcaat catgtcgcaa
     1921 cgtacgatac cgccgaagat gttgatcaat acgcctttaa cggatttgtc ttccaaaatc
     1981 agtttgaacg cttcaaccac gcggtctttg gttgcgccgc cgccaacgtc caagaagttg
     2041 gcaggttggc cgcctttgag tttgatgatg tccatggtgg ccatcgccaa accggcaccg
     2101 ttaaccatac agccgatatt accttccagg gcaacatagt tcaggtcgaa ttcagaagct
     2161 ttcagttcac gttcgttttc ttgagatttg tcgcgcaatt cagcgatttt tggcaggcgg
     2221 tagagcgcgt tgctgtcgat gccgattttg ccgtccacgc aagccagtgc gccgttttcg
     2281 cgaactgcca gcgggttgac ttcaaacagg gcgaagtcgt tttcgacaaa cgctttgtac
     2341 gcgccggtca tcagtttggc gaactcgttg atttgtttgt ctttcaagcc cagttggaag
     2401 gcaacttcgc gagcttggca aggttgcagg ccgaccagcg gatcaacggt tactttgaag
     2461 attttttctg gagtttcagc agcaactttt tcgatttcca cgccgccttc ggtagaagcc
     2521 atgaatgtaa cgcggcgggt agaacggtca accactgcgc ccaagtacag ctcggtttga
     2581 acaggataca tgtcttcgca aaccaaaacg ctgttgacag gttggccgtt ggcatcggtt
     2641 tggtaagtta ccaagttggt gccaatcagg ctttcagcca cttctttggc tttttcgcgg
     2701 cttttaacga cttttacgcc gcccgcttta ccgcggccgc cggcgtgtac ctgtgctttg
     2761 acaacagcga atttgccgcc caatttgtcg taagctgcag cggcttcttc gccgttgtgc
     2821 gccaaaatac cgccttgtac gggcaaaccg tagctagcca gcagttcttt agcctgatac
     2881 tcgtgtaaat tcatggattt ctccttaagg tgagtgggtt ttaggaatcg ccctgcggcc
     2941 aatcccatat tttcagacgg tcctactatt tactgaaggg caaccacatc cgcgccagca
     3001 gatacatgac tgcaccgcct cccccgagaa taaaaaagac gatgcctttt ttacgcgaat
     3061 tcggacagag cagataaacg gcaaggctga aactgataaa caacaatgcc acgcgccaaa
     3121 ccaattcttt atctttgaaa cggttgagcg tataagtttc ggtcatcatc acatacatct
     3181 gccacaatgc ggccatacac atgctgatga tgcccatcgg tataaagaaa ataccgacta
     3241 tttctttgtt cataatgaat gtaaatgctg tttgtctgac ggaattataa atcagaaaac
     3301 gaagctctcg gaacattcaa tttgccggag gccttaaggc cgtctgaaaa tttcagacgg
     3361 ccttaatgtc tatcaaccgt gcaaagcgcg tttgtcggcc gccaatgcag cttcgtgaac
     3421 cacttcggac aaggttgggt gggcatggat aatacgggcg atatcttcgc tgctggcgaa
     3481 gaattcgagc gcagtcacgc cttcggtaac caattcgctg acaaccgggc cgatcatgtg
     3541 tacgcccaag atacggtcgg ttttggcatc tgccaacact ttaaccgtac ctttggcttt
     3601 gcccattgcc aatgcacggc cgtttgcacc gaaacctgaa gtaccttttt tgtactccac
     3661 gccttcggct ttgagctgct cttcggtttt acccacccaa gcgatttcag ggtcggtgta
     3721 aatcacaaac ggtacgttgt tgaagtcgat atgcggtttt tgaccggcaa tgcgctcggc
     3781 aacggcaacg ccttcgtcgc tggctttgtg tgccaacatc gggccgcgaa ccacgtcgcc
     3841 gattgcccat acgttaggca ggttggtacg gcattcgcca tccactttga taaagccgcg
     3901 ctcgtctttt tccaagccta cggcttccgc gttcaggcct ttggtgtttg gaatacggcc
     3961 gatggcaacg atcagtttgt cgaatacttc ggttttggct tcgccggcag cagtttcgta
     4021 agcaacggaa acacctttgc cttcagactt gatgtcgccg attttcgcac ccaattcgat
     4081 gctcagacct tgctctttgg tgaagtattt gaaggcttct ttggcgattt gttggtcggc
     4141 ggcagccagg aaggtcggcg cggcttcaag aatggtaact tccgcaccca cgcggttcca
     4201 tacggaaccc atttccaaac caatcacacc ggaaccgatt acgccgagtt tggcaggtac
     4261 ttcggtcagg ttcaatgcgc cttcgttgtc caatacgttc acgttgtcga tagcgacttg
     4321 cggcaacgga cgcggtacgg aaccggttgc tacgatgacg tgtttggctt cgataacggt
     4381 tttctcgcct ttgttatcga cttcaatttg gtaagcatcg ccatttttac cggcaaagga
     4441 agcagtaccg aacaggctgg ttactttgtt tttttggaac aggaatttca cgccgccggt
     4501 cagtttggtc acgatggcat ctttgcgctc aatcattttg gccgcgtcaa attttacgtc
     4561 gccgacagtg ataccgtgtt cggcaaaatc gtgttgcgca gcgtggaaat gttcgctgga
     4621 ttgcaacagg gctttagaag ggatacagcc tacgttcaag caagtaccgc ccaatgcagg
     4681 ggcattgcct gctttgttaa cgcctgcatc gacacaggca gttttgaaac ccagttgtgc
     4741 ggcacggatg gcggcaacgt atccgcccgg acctgcacca atcactacta catcatattg
     4801 agacatcgtg tcatcctttg cttttagcag tatctcaaaa taaaaacgat acttttcgtt
     4861 tcttttttta ttttgaatga ctgcgttttg atggttttaa gaaaaagccg tctgaaagtc
     4921 catatctgcc gattcattcg aatcaaatgt ttcagacggc ctcaatcgtt cgttgagcct
     4981 attgtagcac gatactacat tacgctacat ctaaataaaa attcagacgg ccttttcatt
     5041 aacaaataaa aagccgtctg aaacgattac agatccaaca acaggcggac cgggtcttcc
     5101 aacgcgtctt taatggctac caaggtcaat acagcttcgc ggccgtcaat gatacggtgg
     5161 tcgtaagaca gagccagata catcattgga cggacaacaa cttggccgtt ttcaaccaca
     5221 gcgcgctctt tagtggcatg catacccaaa atcgcagatt gaggcgggtt gatgatcggg
     5281 gtggacatca tagaaccgaa agtaccgccg ttggtaatac tgaatgtacc gccggtcagg
     5341 tcttcgatag cgattttgcc gtctttggct tttttcgcgt aatcaacaat tgcttgttcg
     5401 atgtcggcaa tgctcatttg gtcggcatcg cgcaggattg gtacaaccaa accgcgtggg
     5461 ctgccaattg cgataccgat gtcgaagtag ccgtggtaca caatgtcttt gccgtcaaca
     5521 gaagcattca caaccgggta ttttttcagg gctgtaacag cggctttaac gaagaaggac
     5581 ataaagccca gttttacgcc atgttctttc tcgaattttt ctttgtactt cgcacgcaag
     5641 tccatgatcg gtttcatgtt gacttcgttg aatgtagtca gaatggcgtt ttcttgttga
     5701 gaagccaaga ggcgttctgc aacacgggca cgcaggcggc tcattggtac gcgttcttca
     5761 ggacgtgcgc cggcaggaag tgcaacggca ggggccacgg ctgcggcagg tttggcagcg
     5821 gcattttgta cgtcttcttt caatacgcga ccgtcacggc cggaaccttg caatacgttc
     5881 acgtcaacac cggtctcggc agccagtttt gcagcggcag gcatggcggc gttgttttgt
     5941 gcagcagcag gaacggcagc cggggcagct tcggcaggag cggctgcagg tgcttcagca
     6001 gcgacagtag cagctgtatc gatacgggcc aaaacttggt cggcaacaac ggtttcaccg
     6061 tcttgcgcta cgatttcaac caatacgccg gcttgtggag aaggtacttc caaaaccact
     6121 ttgtccgttt cgatatcgat caggatttcg tcacgggcaa ctgcttcgcc aacttttttc
     6181 ttccattcca agagtgtgcc ttcagatacg ctttcagaca gcataggtac ttttacatca
     6241 ataatcattt tgtgtctcca atggcccttt cagacggcct gttgtgtgtt tttaattctg
     6301 catatgggca ttccgcctga gcagactcag acggaatgct acttggttta taacgccaaa
     6361 gcgtcttcaa ccaattgttt caattgagca atgtgtttgc ttgagtagcc cactgcaggc
     6421 gatgcgctgc ttggacggcc ggcataagac agtttttgct cttcgctgat aacgtcttcg
     6481 atgcggtggc ggatttggta gaacgcgcct tggtttttcg gctcttcttg cgcccaaacc
     6541 acagattttg cgttcggata tttcgccagt tcggctttaa cctcgtcgta tgggaacgga
     6601 tacagctgct caacgcggac gatagcgaca tcatcttcca gtttacgttc ggctcgaccg
     6661 gcttccaagt catagtaaac ctgaccggca cacaatacca cgcgtttcac gctgtcgttg
     6721 cttgcgcgtt cggcggtatc accaataacc ggacggaaag tcgaaccttc ggtgaagttt
     6781 tccagcgggc tcattgcacc tttgaagcgc aacaggcgtt tggacatgaa aatcaccagc
     6841 ggtttgcggt atgaacccaa gacttggcgt tgcaagagat ggaacatttg cgacgcttca
     6901 gacggcatga tgacttgcat attgttctca gaacacagtt gcaaccaacg ttctacgcgt
     6961 gcagaagagt gttcagggcc ttgaccgtcg tagccgtgcg gcaggatggt ggtcagaccg
     7021 cacaagcgac cccacttggt ttcgcctgaa gacaggaatt gatcaatagt tacttgcgcg
     7081 ccgttggcga agtcaccgaa ttgagcttcc caaatggtca gcttgtcagg agcggagcag
     7141 gcaaagccgt actcgaacgc catcacggct tcttcgttca agatagagtc gataaccagg
     7201 aactcgccca agccttcgcc catgttgcga agaggaacat aagtaccgtc gtcccatttt
     7261 tcgcgttttt gatcgtgcag aacggcatga cggtgtgaga atgtaccgcg gccggagtcc
     7321 tcaccggaga tacgcacgcc atgacctttg gtcagcaggc ttgcgtatgc gagggtttcg
     7381 gccatacccc agtcgatggc ttgtttgccg gatgccatgg ctttacgcgc ttcgatcaca
     7441 cgttttgcag tcggatgcag ggcaaagcct tccggcacag cggtgaattt ctcagtaaga
     7501 cgctcaatgt cggcggcagg caaaccggtt tcgatttttt cgcgccaatc tttaccttgg
     7561 tatttgctcc agtcgatttg tgtgcgttgg aagttgctca acgttgtttg ctcaacatgt
     7621 tcgcctttgt ccaaagcatc acggtaggct tggatgtaac cgtcggcctc aacttgagtt
     7681 accacgcctt cggcaatcag ttgctcggtg tacaaagcac gcgcacccgg atgttgcgat
     7741 acttttttgt acatcatcgg ttgggtcaaa gtcggatcat cgccctcgtt gtgaccccat
     7801 ttacggtagc agacaacgtc gattacgatg tctttatgga attttttgcg gtaatccaaa
     7861 gcggcttgaa tggcaaagca aacgcgttcg ggatcatcgc cgttcacatg gataaccgga
     7921 gcggaaacca tttttgcgat atcggtacag tgtacggttg aacgggtatc gcggatatcg
     7981 gaagtggtaa agccgatttg gttgttgatg acgatatgga ccgtaccgcc tgtggtataa
     8041 ccgcgcgttt tagacaggtt gaatgttgct tggttgacac ccaaaccgat aaatgcggaa
     8101 tcgccgtgaa tcaataccgg caagacttta tcgcggccgt tttcgcccaa acgtttttgt
     8161 ttggcgcgtg cagaaccttc cactaccgga ttgacgattt ccaagtgtga cgggttgaac
     8221 gccaaagaaa cgtgcatcgg gccgtgaggg gtggcaatat cggagctgaa gcccatatgg
     8281 tatttcacgt cgccactagg cagtttgatt tcggcacggc cttcaaattc ggcaaacaaa
     8341 tcgccgggtt ttttgcccaa aatgttcacc aaaacattca gacggccacg gtgcgccata
     8401 ccgatgatga cttcttccac gccgtcctta ccggcgtttt gaatcaggta gttcaaaccg
     8461 gcaatcgcgc tttcgccgcc ttcgacaccg aaacgtttct gaccgacata tttggtatgc
     8521 agataacgtt ccaaagtctc ggcagcggtc atctctttca agatacggcg tttttgatcg
     8581 gcattgtaat gcggtgtgga caatacgctt tcaaaatagt tgcgtaccca acggcgctct
     8641 tcggtattgg gaatatagat atattccaat gcgatgtggc cgcagtaggt ttgtttgagg
     8701 ttgctgatga tttgggacaa gggcagtttg ccgcgattgg caaaatcacc ctcgcccata
     8761 ttgaatcgaa gcgccatatc ggcatctgac agaccgtgga atttcggatc gagggcttca
     8821 atatcgcgcg gagggatacg tttgagcgga tcaagttggg ccgcacccac gccttggata
     8881 cgataggcag aaatcagccg taaaacgctg acttgctttt tcagcattgc ctcatccgca
     8941 ccgcccgcaa cggcagatgc aattttcttt ttcgccaaag taacaaatga ttcgcgaatc
     9001 ggtgtgtgtg cgacatcgac agcaaccgtc cccggctgtt tgctcaaatc ggtgaaatac
     9061 tgcttccatt tttcatcaac cgcatcgggc ttttccaaaa aagcctcgta caattcctca
     9121 atgtagggtg cgttcgaacc gaacagataa gagaaattga gtttttcgtc catcatggcg
     9181 tgcgctcttt tctttaaaat ataaacaaag ggtagaagtc cgcgtcctga cggctgaact
     9241 gatgccgtct gaaaagacat tttattctac cctattttaa accggattgc ttgtcttgat
     9301 ttcatccgac tacaatcaga cggattcagg aaacaatccg gttctctgat attgcctgtt
     9361 tgacaatata ttaatgtatg ttacctctcg cctgccggca cataatcgcg gcgttccgaa
     9421 ccggtataaa gctggcgcgg gcggccgatt ttcagcgaag gatcgctaat catctcgtgc
     9481 cagtgcgaaa tccagcccac gctgcgcgac agggcgaaga tgacggtaaa catttcggtc
     9541 gggatgccca gcgcggacag gacgatgccg gaatagaaat cgacattggg atacagtttg
     9601 cgttcgataa agaacgggtc tttcagcgca atctgttcca attccatcgc cagtttgaat
     9661 ttcggactgt cttccaagcc caattccttc aaaacttcat agcaggtttc gcgcataatg
     9721 ctggcacgcg gatccatatt gcggtatacc cggtgtccga agcccatcag gcggtatttg
     9781 cgctgtttca cgccttccat gtatgcggca acattggaca catcgccgat ttcatccagc
     9841 attttcaaca cggcttcgtt cgcaccgccg tgcgaagcac cccacaggca ggcgataccg
     9901 gcagcaatac aggcaaacgg gttcgcaccc gaagagcctg ccagacggac ggttgaagtt
     9961 gaggcgtttt gctcgtggtc ggcatgcaaa ataaagatgc ggtcgagtgc gcgtgccaaa
    10021 acgggattag gtttgtagtc ttcacacggt gtggcaaaca tcatatgaag gaagttttcg
    10081 gaataagaaa gattattctt cggataattg aacggcagac cgtttgaata gcggtaacac
    10141 attgccgcaa tggtcggaat tttggaaata aggcggtaaa tcgcgatttt gcggtgttcg
    10201 ggattagtaa tgtccaagct gtcttggtag aacgcagaca gcgcgccgac cacgccgacc
    10261 atcatcgcca tcggatgcgc gtcgcggcgg aaaccccgga agaaccaagt cagctgttca
    10321 tgcaccatcg tgtggcggcg gactgtattg tcaaattctg ccttttgctc gggagtcggc
    10381 agttcgccgt aaatcaacag gtagcagact tccaaataat cggacttttc ggccagctgc
    10441 tcgatggggt atccgcgata ataaagcaag ccttgatcgc cgtcgatgta agtaatttta
    10501 gactcacagc ttgcggttga aacaaatccg gggtcgaagg caaacaaacc tgtatttttt
    10561 gtcagccccc gaatgtcaac cacatcatgc ccgatgctgg cttccaatac cggcagctcc
    10621 aaacctgccc gacccggtac gttgagtttg attgatttgg acatattatc gctcctttgc
    10681 aagttataac ccacgttact gcatccgcag cacgttcttt gctttcagac ggcctttgca
    10741 ttttgaaatc tgccctcaga caaatttcag atgcctcatg cccgtctgat tttttcaagc
    10801 atcgggataa ggtgcccttt gtccgtttcc gaatgcccgt gaatcaaggc aagcaattct
    10861 tgatcttgaa attcaaggat ttcggaaaac tcggacagct ctttatcgct caaatgctcg
    10921 aattcttttt ccataaacct gccgaagatt aaatctagtt ccaacaatcc ccggcgggtt
    10981 tgaaaacgga ttttccgttt ggcaatgtcg tcaaaaacca tcatttctta aacaacccgt
    11041 ttcaacataa tctctttaat cttaccgatg gcccgggtcg gattcaagtg tttgggacat
    11101 acgtccacgc agttcataat ggtgtggcag cggaacaaac ggtacggatc gttcaaatta
    11161 tccaaacgct cattagtgat ggtatctcgg ctgtccgcaa tgaagcggta ggcgttcagc
    11221 aagccggacg gaccgacgaa tttgtcggga ttccaccaga atgacggaca ggcggtcgaa
    11281 cagcaggcgc acaaaataca ttcgtacaaa ccatccaact ctttgcgctc ttcctgagtt
    11341 tgcagacgct ctttgtccgc atcaatcgga ttgtcgttaa caacataagg tttgacggaa
    11401 tggtattgtt tgaagaactg ggtcatatcc acaatcaggt cgcggatgac gggcagaccg
    11461 ggcagcgggc ggattttgac aggctgtttc aagctgcgta aatcggtcag acacgccaag
    11521 ccgtttttgc cgttgatgtt cataccgtcc gatccgcaaa tcccttcgcg gcaggagcgg
    11581 cggaaagaca aggtatcgtc ttgtgctttc aggcgtacca aagcgtccaa aagtttcacg
    11641 tcggtcggtt ccaattccaa ctcgtaacgc tgcatataag gcttggcatc aacgtccggg
    11701 ttgtaacggt aaatttcaaa actcattttt tccatgagaa gtgttccttt tctcatcaac
    11761 tgcccaatga ttttgtatta taaattcaag cagttcaatg tgggtatggt taggtttcag
    11821 acgacctttg aggcgaagac cgtctgaaaa cgcatcaata aacgcgtttg gccggtttga
    11881 tgtattccac gctcaaaggc ttggtgtgta ccggtttgta agacaaggta ttggtgtccg
    11941 aatgatacag ggtgtgtttc atccaatttt cgtcatcgcg ctcaggatgg tcgtctgaag
    12001 cgtgcgcacc gcgtgattct ttacgtgctt cggcagacac caaagtcgct ttcgccactt
    12061 caatcaggtt atccaattcc aaagcttcga tacgcgcggt attccacact ttgctcttgt
    12121 ctttgatttc ggtacgtttc acacgctcgg caatcgccat gatttcttga acgcctttgc
    12181 tcagaatctc atcggtacgg aacacgccgg cgtgcagttg tacggagcgt tgcagttcgc
    12241 gacgcaatgc atcaacgttt tcaccatcgg tttgactgtc caaacgctcg atacgttggc
    12301 gggtcaactc accggcatta gcaggcagag gtttccaatc gctttgctct ttgatgaatt
    12361 taatcatgct gtcaccggca gctttgccga acaccaccaa gtccagcagg gagttcgtac
    12421 ccaaacggtt cgcaccgtgt acggaagcac aggcgcactc acctgcggca tacaggcctt
    12481 ttacaggtac ttcgtactcg tcgccttgcg gaacaacaac ttcaccgtga taattggtcg
    12541 gaatgccgcc catcatatag tgggtagtcg gcacaaccgg aatcgggtct ttaatcggat
    12601 cgataccggc aaactgaatg gaaatctcgc ggatgcccgg cagtttttcc ataatttttt
    12661 ctgcaccgat atggtcgatt ttcagtaaga cgtggtcttt gtttttacca cagccgcgac
    12721 cttcatagat ttccatcgcc atcgcgcgtg aaaccacgtc gcgagaagcc aagtctttta
    12781 cggtcggcgc atagcgttcc ataaagcgtt cgccgtcggc gttcaacaga ataccgccct
    12841 cgccgcgtac gccttcggta atcaacacgc ccgcacccgc cacgccggtc gggtggaatt
    12901 gccagaattc catatcttcc aacggaatgc ccgcacgggc gcaaatgccc aaaccgtcac
    12961 cggtattcat ataagcattg gtagaagaag cataaatacg accgccgcca ccggtagcaa
    13021 acatcacggc cttggcgtgg aaaatataaa cttcgcccgt ttccatttcc atggcggtta
    13081 cgccgacgac atcgccgttt tcatcacgaa tcaaatcttg cgccgtccat tccacaaaga
    13141 attgtgtatt ggcacggacg ttttgttggt acaaagtatg caacatcgca tgaccggtac
    13201 ggtcggcaac cgcacatgca cgttctaccg cacgtttacc atgttcggca gtatgtccgc
    13261 cgaaaggacg ctgataaatt ttgccgcttt caacgcggtc aaaaggcata cccatgtgtt
    13321 ccaactcaat caccgcttca ggcgcagcgc gacacataaa ctcaatcgca tcttggtcac
    13381 ccagccagtc ggaacctttc acggtatcgt acatgtgcca gtcccaacgg tcctcctgca
    13441 cattacccag agaggcggaa ataccgccct gcgccgctac ggtatgcgag cgggtcggga
    13501 acactttaga caaaacggca caattcaaac cggatttgga taattggagg gctgcacgta
    13561 aacctgcacc gccaccgccg acaatcacgg catcaaactt gcgaacagga aaacccatac
    13621 ttacccccaa atcactttaa ctgaatacac gaggcagccg accagccaga caatggtggc
    13681 aacctgcaaa aacaaacgca cgccgaaggg tttgatatag tccatccaca aatcgcggat
    13741 acccacccaa gcgtgcaaga atacggcgat aaagctcact tgggtaaata cttttaccca
    13801 agcttgacta aaaaatgcct gccatgccgg atattcttta ggcagggcaa atagaaccac
    13861 taaaagtgca acggtataaa tcaacataat aaccgcagtc gcacgctgca ttacccaatc
    13921 gcgcaaaccg taatgggcac cggtcaattt acgttctacc ataacaacgc tcccaaaacg
    13981 acagtcaaaa ccaatgcaga agcaaatacg gctttagcgg tattgcgcgc agtattcagc
    14041 tcaaggcctt tgtgcgcatc caaaaataaa aagcggatac cggcgagaga atggtgcaga
    14101 taagcccaca atataccgat taaaaccagc ttgaccaaag gatgggaaac aatggcacgg
    14161 taagtttcaa atgcagactc ttgactcagg gtaccggaca gaaaatacag caggaaaggc
    14221 agcataataa acagcccgac cccgctaatg cggtgaagga tggaaactat cccgggtatc
    14281 ggcagacgga tgttcggcaa atccagataa acaggacgca gtttgacaga catagtcaat
    14341 tcctctgtag ttatatatcg actcaaaaac cgtaaaccca ttgctacact gggttacatc
    14401 aacgttaatt tacctgtttt atcccatttt gaacagtgta ttttaaaaac aaactgatag
    14461 attttgacaa acacgcgtgg aaacagcaga ttaaaagaaa gatttttagt tttgccgctc
    14521 ccttcccgac acggtatcag catccgaccc ccaaccccat ccccaaaaat aaaatgccgt
    14581 ctgaaaaaaa tttcaggcgg cattgttttg ccatatcggt caggctttca atttcgcatc
    14641 ggcatcgcgc aacgcggtac gcagaccttc ctcgataaca ggatggtaga acggcatatc
    14701 cagcatttgc ggaacggtca tcttcatttg atgcgcccat gccaacaagt gagccaaatg
    14761 ttcggcggca gggcctacga tttccgcgcc gataaagcgg ccggtggctt tttcggcata
    14821 caggcgcata tggcctttgt ttaccagcat cacgcggctg cgaccttgat ttttgaacga
    14881 tacttcgccg atgacaaatt cgtcggcttg gtattgcgcg gcaacctgcg cgtatttcaa
    14941 accgacaaag ccgatttgcg gactggtaaa caccacgcca atggtgctgc ggcgcaaacc
    15001 gctgccgata ttcgggtagc ggcccgcgtt atcgccggca atcttacctt ggtcggcggc
    15061 ttcatgcagc aggggcagtt ggttggacgc gtcgcccgca ataaagatat gcggaatgct
    15121 ggtctgcatg gtcagcggat cggcaacggg tacgccgcgc gcgtctttgt cgatattgat
    15181 gttttccaaa ccgatattgt caacgttcgg acggcgacct acggctgcca acatatattc
    15241 ggcaacaaat acgccttttt cgccatcctg ctcccaatgg acttctacat tgccgtctgc
    15301 gtcgagtttg acctcggttt tagcatccag atgcagtttc aattcttctc cgaacacggc
    15361 tttcgcctcg tctgaaacaa cggggtcgga aatgccgccg atgattccgc ccaaaccgaa
    15421 aatttcaact ttcacaccca aacggtgcaa tgcctgaccc agttccaaac cgataacgcc
    15481 cggcccgaac acggcaacgc gcttaggcag cgtatcccac gagaaaacgt cgtcgttgat
    15541 aatcaaacgg tcgcccaaag actgccactg cggcaggatg acgggacgcg aaccggtagc
    15601 aatcacgaaa ctttttgccg caatttggat atggtcgtcg atttggacgg tatgctcgtc
    15661 gatgaattta gccgagccca tgatgcgctt gtcggcaggc cattcttcca catcggtaac
    15721 gacaaagccg acaaaacggt cgcgctcgga tttgacgcgc cgcataacct cttctccgtt
    15781 gacgacgatg ctgtctttgt ccaaatgcac gccgaagggg tcggtatgca atgcgtggtg
    15841 acgcgcttct gcggcggcaa tcaagagttt ggaaggcata cagcccacgc gcgcgcaggt
    15901 tgtgccgaac acattgtttt caatcaggta aacattatcc gaatgtaaac gggcattgcg
    15961 aaacgcaccc ataccggcag taccgccgcc gattacgacg atatccgctt gaattttttt
    16021 cataatcttt gtccttccgt tttcgtctga aacattgccg acacagaaca tatcggtttc
    16081 agacagcatt taaatatctt cccaatcaaa aacattattc agataaataa aaacaaaatt
    16141 tcgattgagt gcgacaatat ccaaatcttc ttgtaaatat agagttattc ggagcggcag
    16201 tatagaaccg cccgaataac tctatccggt atatcagaca gtttaatcaa actgccttaa
    16261 gcaaacagct atcagttttt agccaagtaa gcttccaaat cttcgctgcc gccgatgtat
    16321 ttgccgccga tgaagacttg aggggcagtc atcttgccgg taatagcgcg aacggaagta
    16381 acggttgcat ctttgcccaa tacgatttct tcgtaagaca aacctttgtc ttgcaaagct
    16441 tgtttgactt tggcacagaa ttggcaacct ggtttagtga aaattgccac agactcttga
    16501 gccttccaat cgggagcaac gaatttcagc atagtatctg catcggatac tttgaaagga
    16561 tcgcccggtt cttcaggttc gatgaacatt ttttcaacca cgccgtcgtt aaccagcatg
    16621 gagtaacgcc aagagcgttt gccgaagccc aagtcttctt taccgaccag catacccata
    16681 ccttcggtaa attcgccgtt gccgtcagga atcatgtaga tgttgtctga ttcttcttcg
    16741 gcagcccaag cgttcattac gaaagtatcg tttacagata cgcagcagat tgcgtcaacg
    16801 ccgttttctt tgaacgcgcc gaataattcg ttgtaacgcg gcaggtgtga agaagaacaa
    16861 gtcggggtaa atgcaccggg cagggagaat acgactactt ttttgccttt gaacaaatca
    16921 tcggtagaca catctttcca agtgtcgccg acgcgggtgc ggaatactac ggaaggtact
    16981 ttttgaccgg tacgatcttg caaagccatt ttgagctcct taatagattg gtttgagaaa
    17041 taattgtgaa tcgccgcgca ttctacccca tgcaaaatga tttgtataat ttatagttta
    17101 aataatataa attatttaaa actatcaaat acaactccga tttgcctgtt ccccacatat
    17161 aaagccgaat tagagaaaaa caagctgcaa tgaaaagatt ttccggcaac tgctcccctg
    17221 cgccgcaact caggacaaaa tgccgtctga aaggctttca gacggcattt gttcatacgg
    17281 tctcggctta tttcgccaat tcggcacgca gtttgcgggt tacgttcatc attacttgaa
    17341 gctgttccag agtttctttc cagccgcgtg ttttcaggcc gcagtccggg ttaacccaca
    17401 gacgctcaac cggaacaacc tcaatggctt tgcgcaacag gtgttccact tcggcttctg
    17461 tcggtacgcg cgggctgtgg atgtcgtaaa cgcccgggcc gatgtcgttc gggtatttga
    17521 attcgccgaa cgcagccaag agttccatgt cggaacgtga agtctcaatg gtgatcacgt
    17581 ccgcatccat tgcagcaatc gcaggcagga tgtcgttgaa ctcagagtag cacatatgag
    17641 tgtggatttg agtgctgtct tcgcaacctg cagaggatag gcggaaggat tcgcccgccc
    17701 agttcaggta ggcatcccaa tcggcacgtt tcaaaggaag accttcgcgg atggcaggtt
    17761 cgtcaatttg gatgacttta atgccggctt tttccagatc caatacttcg tcgttcagag
    17821 ccagtgcgat ttgtttgcac acggtagcgc gcggaatatc gttgcggacg aaagaccatt
    17881 gcagaatggt tacaggaccg gtcaacatac ctttcatcgg gcgtttggtc aggttttgtg
    17941 cgtaagtaga ccaagcgaca gtcatagctt caggacggct tacgtcaccg aagatgatag
    18001 gtggtttaac gcagcgtgag ccgtagcttt gcacccagcc gtattgggtg aatgcaaaac
    18061 cgctcaacag ttcgccgaag tattcgacca tgtcgttacg ctcggcttcg ccgtgcacca
    18121 gtacgtccaa gtccagtttt tcttgctctt caaccaccaa ggcgatttct tttttcattg
    18181 cggcttcgta atcggaggca gacagttcgc cttttttgaa ggctgcgcgt gcttggcgga
    18241 tttcggtagt ttgcgggaaa gaaccaatgt tggtcgtcgg cagcagaggc aggttcaacc
    18301 atgcttgttg cgctttgata cggtcggcaa acggtgattt gcgttggtct gcgttgacag
    18361 gcaggtcggc caagcgtttg gcaacgtctg cacggtggat ttcactgctg ttggcacggg
    18421 agtcggcagc agcttggctg gcggccagtt cttcggcaac agaatcacgg ccttcattca
    18481 atgcggcttt cagaacacgc aattcttggg ttttttgcag agtgaatgcc aaccaagagt
    18541 acaggtcggg tttgttggct ttcagttttt cttcgactga caagtcaaac ggagtgtgca
    18601 gcagcgagca agagctggaa atccacaaac gctcgcccag tttggcttgc agaggcccga
    18661 cggtttccaa aactttgttc aggttggcgc gccaaatgtt gcggccgtca atcacgccgg
    18721 cagacaggac tttgtcgtag ccggcgaacg cgtccagttg ctcgggtgcg cgtaccaagt
    18781 cgatgtgcag gccgtcaaca ggcagggatt tcaacaatgc ggcgtgttcg gcaacagaac
    18841 cgaagtaagt gcccaacagg attttggcgc ttactttgtt caaagtagcg taaacgtctt
    18901 tgtatgcttc tacccattct ttaggcagat cgacagccaa agcaggctcg tcgatttgaa
    18961 tccactcggc accggcttca accaaagcag tcaggatttc aacgtaaaca ggcaacagtt
    19021 taggcaacag gctcagacgg tcgaattcaa cggagccttt ttctttaccc acccacagga
    19081 aagtcaaagg accgacaacg gtcggtttgg ctttcaagcc caaagtttgg gcttcttgca
    19141 gttgttgaac gtagtgtttg gcgttggctt tgaattcggt atcggcatgg aattcaggca
    19201 ccaggtagtg gtagttggtg tcgaaccatt tggtcatttc gatggcgaat tggtctttgt
    19261 tgccgcgcgc cagttggaag aattgttcca aaggcaggtt ttggctatcg aagccgaaac
    19321 gggcgggaat cgcgccggtg gcaacttgca ggtcgaggat gtgatcgtag aaagtgaaat
    19381 cacctacagc aacgtaatca gcgttggcag cagcttggtg tttccagttt ttctcgcgca
    19441 ggtctttggc aacgtcgagc agctcttgtt cactgatctc tttgcgccag tatttttctt
    19501 gggcgaattt caattcacgg aaggcgccga cacgcgggaa gcctgagaaa tgtaatgttg
    19561 tcatgttgaa ctctccagtt ggattttgta aggaccggtt caatccgaat cggccggtat
    19621 tgtaaatcag aatggctcgg atagggcggc gttttgcttt tcagacggcc tcggatgctg
    19681 tctgaagccg ttttcggctt taagggcgcg cgcctaaaat atggcagatg gcgtaagtca
    19741 gctcgctgcg gttgagcgtg tagaagtgga aatctttcac gccttcgcgg gacaggactt
    19801 taaccatatc gatggcgata ctggcggcga cgaggttgcg cgtgccttgg tcgtcgtcca
    19861 agccttcata catttgcgac agccatttcg ggattttgac gttggttact tgtgccattt
    19921 tgccgagttg cttgaagttg gtaacaggca ggatgccggg aacgatttcg acgtcgatgc
    19981 ccaacatcac gcagcggtcg cggaagcgca ggtagcgttc cacatcgaag aagaattggg
    20041 tgatgacgtg gttcgcgccc gcatcgattt tgcgcttcag attaatcagg tcggcttgtg
    20101 cggatttcgc ttcgggatgt acttcggggt atgctgctac agagatgtcg aagtcggcga
    20161 cggagcgtaa tagcttaacc aagtcttcgg cgtaaaacgg ttttttctca taaccggccg
    20221 gctcgtcgcc acgcagggca acgatgcggc ggataccgct gtcccaatag tctttggcga
    20281 tttggcgcaa ttcgtcgtgg gatgcatcga tgccggtcag gtgcggagct gcttccaagc
    20341 cggtttcctg tttgatgcgt ttgacgatgc cgtgcgtgcg gtcgcgttcg ccggagtttg
    20401 cgccgtaagt tacggatacg aacttgggat gcagggtttg cagacggtgg atggaatccc
    20461 acagcatcgt ttccatttgt tcgtttttcg gcgggaaaaa ttcaaacgaa acgttgatgt
    20521 tgcctttcaa atcggaaagg ctgttgttta acgcattgat ttcttttgca taatttatct
    20581 gcataccttc gttatctttt acatgatgtt tcaggcggta tgataaggtt gcaccgaata
    20641 tgagtcaatt tcaaaatttt caggtttatg atgaaaattt ttaatattta gcaaaaacta
    20701 tacgcttttc ttgtacaagc cttctaaatt atgataaagt ttgttatgtt ataacacttc
    20761 aatcaaggca atatatgaaa ccgaatattc atcctgacaa ttatcgaacc gtcctttttt
    20821 tcgacagcag tgccaatgaa ggctggctga ttcgttcttg cgccggaacg cacggaaaaa
    20881 caatggtttg gacagacggc aaagaatatc cgctcttttc cttagatact tcctcttcct
    20941 cacatcccgt ctataccggc aaacagcgta acgtcaatac cgaaggccgt gccagcaagt
    21001 tcaaccaacg tttccaatcc gtcatgtcat cgtttagaaa ggacaaataa tgcaggttct
    21061 gtcttcgctc aaaaccgcca aacaacgcca ccgcgactgc caaatcgtca gacgcagggg
    21121 caaagtgtac gtcatctgca aaagcaatcc gcgttttaaa tcgcgccaac gctgaccccc
    21181 tgcccttcct gcttcggttg taaagcccct cctaccgcaa gcccttttca aatatgccgt
    21241 ctgaaacaga aaaatgcgtt tcagacggca tttttccgtc gatacgaatc cggaatgtgt
    21301 ttgaaataca gcataaacag ataccaaaac agcgtcgcgg cggctgcacc gagcagtacc
    21361 catgcgctga tttcggcgac ttcgcgcagg acacgtttct gcctgcggct aaagaggcgg
    21421 ttgataatca gcgcggcaag tgcgaaaaag aaaaaaatac ggaaaagtct gcctatcatg
    21481 tcgggaagcg aaaagtatgg ttcgggcgcg gcggcatggg aaaggatgtt tcagcgcgtc
    21541 aggctgcttg tccgaccgtg tcaaaaagtc atgccgtttg cgatataata acgcttttcg
    21601 ccgcatttgt tggaacagga tatgagtcaa aatacctcgg tgggcattgt aacgccccaa
    21661 aaaattccgt ttgaaatgcc gctggttttg gaaaacggta aaactttgcc gcgtttcgat
    21721 ctgatgattg aaacctacgg cgagctgaat gctgaaaaaa acaatgcggt tttaatctgc
    21781 cacgcgctgt cgggcaacca tcacgttgcg ggcaggcatt cggcggagga taaatatacg
    21841 ggctggtggg acaatatggt cggtcccgga aaaccgattg atacggaacg ttttttcgtg
    21901 gtcgggttga acaatctggg cggctgcgac ggcagcagcg ggcctttgtc gatcaatcct
    21961 gaaacgggca gggaatacgg cgcggatttt ccgatggtta cggtgaagga ctgggtaaaa
    22021 tcacaagccg cgcttgccga ttatctcggc atcgaacaat gggcggcggt tgtcggcggc
    22081 agcttgggcg gcatgcaggc tttgcagtgg gcgatttcct atcccgaacg tgtgcgccac
    22141 gccttggtga ttgcgtctgc gccgaaactg tccacgcaaa atatcgcgtt taatgatgta
    22201 gcacgtcagg cgattttgac cgaccccgat ttcaatgaag gacattaccg cagccacaac
    22261 accgttcccg cgcgcggttt gcggattgcc cgtatgatgg gacacattac gtatcttgcc
    22321 gaagacggtt tgggcaaaaa attcggacgc gatttgcgtt ccaacggcta tcaatacggc
    22381 tatagcgttg aatttgaagt agaatcctat ctccgctatc aaggcgacaa attcgtcggg
    22441 cggtttgatg ctaatacata tttgctgatg accaaagctt tggactattt cgatccggcg
    22501 gcggatttcg gcaacagcct gacccgcgcc gtgcaggatg tgcaggcaaa attctttgtc
    22561 gccagcttca gcaccgactg gcgtttcgcg cccgaacgtt cgcacgaact ggtcaaggca
    22621 ctgattgccg cccaaaaatc cgtgcagtat atcgaagtca agtccgcaca cgggcacgat
    22681 gcctttttaa tggaagacga agcctatatg cgcgccgtaa cggcttatat gaacaatgtt
    22741 gacaaggatt gccgattatg aacctgcgcg atgatttgca actgatttac gaccggatac
    22801 ccgaaggcag ccgcgtctta gacttgggct gcggcgacgg cgaattgctg gcggctttgg
    22861 cggaacacaa aaaatgcagc ggctacggca tcgaaatcga caccgacaac gtgattgctg
    22921 cgatgtcgcg cggcgtaaat gttatccaag ccgatttgga agaagggttg accgcattca
    22981 acgatcaaag ttttgatgtg attgttttga gccaaaccat ccaagcgatg cagaataccg
    23041 aaaaaatcct gcgctgcctg atgcgcctcg ccaaacaggc aatcgtcagt ttcccgaatt
    23101 tcggctactg gcgcaaccgc gtccaaatcg cactcggcgg acatatgccg gtttccgaac
    23161 gtatgcccta ccattggtac gacacgccca atatccattg gtgcacgctc aaagactttg
    23221 atttgttgtg cgccaaaaac aatatccgcg tcctcgaacg cgccgtgatg acgggcaaca
    23281 gacaggtcaa acatttcccc aaccttttgg gcagccttgc gttttaccgc gtcgggtaag
    23341 acaggttgaa aaccttgtcg gcaataacgg gtttctcggt tctattaaca acagtccgtc
    23401 tgaaaaatga aacggcaggt tttcagacgg ccttttcaat attggaaaca atagaatgaa
    23461 aaacaaaacg tcatcacttc ccttatggct tgccgcaatc atgctggccg cgcgttcccc
    23521 gagcaaagaa gataaaacga aagaaaacgg cgcatccgcc gcttcgtctt ccgcgtccca
    23581 atccgatttg caaccggccg catccgcccc tgataacgtc aagcaggcag aaagcgcgcc
    23641 actgtgaaat tgcaccggcc tgcaccccgc cgccggcatt ggcgatctca tacagcaaat
    23701 cgccgaacac atcgactcgg actgtctgtt tgccctttcc cataacgaac tggaaacccg
    23761 tttcggctta cccggcggcg gctatgacaa catacagcgg ctgctgtttc ccgacatccg
    23821 ccctgaagat cccgactacc atcagaaaat catacaggca attgaagact tgcgttacgg
    23881 aacgcgcacc atcagccggc aggcacaaga tgccatgatg gaacaggaac gccgcctccg
    23941 agaagcgacg ctgctgctga cacaaggcag tcaagaaacc cgcggacaag gcgaggaacc
    24001 gaaacgcgca cgttattttg aagtttcggc aacatctgcc tatttgaacc ggcacaacaa
    24061 cggactcggc ggcaatttcc aatacatcgg ccaattgccc ggctatctga aaatgcacgg
    24121 agaaatgctt gaaaaccaat cactcttccg gctgtccaac cgtgaacgca atcccgacaa
    24181 accgttttta gacatccatt ttgacgaaaa tggcaaaatc acgcgtattg tcgtttacga
    24241 aaaaaatatc tatagtggat taaatttaaa tcaggacaag gcgacgaagc cgaagacagt
    24301 acagatagta cggcaaggcg aggcaacgct gtactggttt aaatttaatc cactatactt
    24361 caatccaaac acggggcgaa tataaaaaaa tgccgtctga aagcctttca gacggcattt
    24421 ttcaagtagg atatcaggcg cgtttgcgga attcgccggt acgggtatcg atttcgactt
    24481 tgtcgccgtt ttcgatgtaa gacatcactt ggatttcggt gccgccgacc agacgcgcgg
    24541 ttttcatcac tttgccggaa gtatcgcctt tgacggcagg ctcggtgtag tcgacttcgc
    24601 gcacgatgat ggtgggcagt tcgacagaaa tgggattgcc ttcatagaag gtaacttcgc
    24661 attggtcttc cataccgtca acgatgaatt tcaacgcgtc gccgatgttg tcggcttcga
    24721 tttcgtattg gttgaattct tcgtccataa agacgtacat cgggtcggca aaatagctgt
    24781 acgtacagtt tttgcgggac aggatgacca cgtcgaattt gtcgtcggct ttgtaaatgg
    24841 tttcggaagc agcgccggtc agcaggtttt tcagtttcat gctgactttg gcggaagagc
    24901 ggccgccttt gatgtattcg gttttttgaa cgaccatagg atcgttgccg accataaata
    24961 cattgccggc gcgcagttct tgtgctgttt tcattgtgat tttccaatca aatcggttgt
    25021 cgtaaaacgc gctattctag cgtatttttt gatgctttga aacaaaggcg gcgagttttt
    25081 cggatgcgga aggctgcccg aaaagataac ggctccaatc ctccgcgcct tgccgccagc
    25141 cgttttgatg ttgttgcagg gtttgccaac attcgaggcg ttgtgttgcg gataaagcct
    25201 ctccgccgtt gaggtcgtcc gaaaggaggc ggtgcaccga tgcggtttcg ggcgtgtaga
    25261 agccgtatgc cttatcccaa aaggcgtgga gtttgtcgag atggacattc tcgtcttgcg
    25321 ggtagatgtg ccaaaaaaag ggttttccgg caagctgggt acgcacgaaa ctgtcttcgc
    25381 cgcgtatcac ggcgcagtcg gcgaggtgca gcaatttgtc gaagtcctgt tgcggcacga
    25441 acgggatttt gacaaggcgg acggatgccg tctgaaagac accgccgtca ttttgcaggg
    25501 cgttttgcgg aataacgccg ctttgtttga ggctgtcgat aatttgcgcc cccgccagca
    25561 gtagggtcat caggctgcct gcctgttgcc acatgtccag ccactttgcc caaacatcgc
    25621 cccgatagcc gaaaagcagc cattcggggg cgtttttttc gggcagcacc agccgccggc
    25681 gcagggcttc ggtatcgaaa cggacggctt cgcggtaatc gcgttcgcgt atcaacccgc
    25741 cgcttttttc gctgaaaccc ataaaccaaa aatatttttg aacgccctcc tgcggcgaag
    25801 gcatcaggtg cagcctttca ttgctttcct ccgcgctcaa atattcccaa ttcagccaaa
    25861 gcggtttgtg tcggcggatg atgttcagca cattttcggg caggtcgcag gcaaaagttt
    25921 cgataacggc atcgggaacg ggcgcggtat caatgtctgc cgcatcggaa tgccaagtgc
    25981 ggacatgaat atcctgatga acgaagggaa catcgggcaa atcgggacaa agcgcgcgca
    26041 aggcggacac gtcgtccgtc cacaaatgca cctgccaacc gagttcgcgg tgcaaaacac
    26101 gggcgagccg ccacgaaacg ccgatgtcgc cgaaattgtc gatgaccttg caaaaaatcc
    26161 aacagacagg aaaagcgtat gtattcataa ccatcgggaa aacaagctaa aacgcgccga
    26221 aatagtggaa caaatacaca accagcaaaa caatcggaat aaacagaaac atggtttgac
    26281 gtttcaaatc aaacccttgt ttaagatgat gcttgatggt ttcaaagagg acgaatttaa
    26341 acaaaaacgt cgcttcgcag gcaaggatga tgccgttgat cagatgctgg ggtttgtctt
    26401 cgggcggatt ggcggcgaaa acggcaagcg tcgccaaggg gatgaggcag acggcgagcc
    26461 agcggacaag caggtttcgg gacattgcaa ggctttcaaa aaagcaaaac gccattatcg
    26521 cctcaagaac gcaccttgtc taacggacgc gccggagatg ccgtctgaag ccttgccgcc
    26581 ggaaaacgcc gcaccaccga acctttaaat atacccggga cgggcgttcc ccgagaaatc
    26641 ggacgctgcc gccgatgcct gcggacaagg cggcatcggg cgcgggcata ttgaaagccc
    26701 gacgcatcgg tttgaacgca ttttataaag gcgtacggca aacccgcccc ttgccgtcgg
    26761 cgtgcttccg caaagcaaaa tataagggcg gcgcgctttc cgctataatc cgcatttttc
    26821 caaacctgaa acattatgcc gaccccgaaa gcctttaccc tgctcgggcc gaccgcctgc
    26881 ggcaaaaccg cgctcgccct caaaattgcc gaaacccttc cggtcgaaat catcagcctc
    26941 gattctgcac tgctctatac cggtatggac atcggcacgg cgaaaccgtc cgcctccgaa
    27001 cgcgcctttg tcccgcacca ccttatcgac atcattacgc ccgttcagac atacagtgcc
    27061 gcccgttttg tcgaagactg cacgcgtctg accggcgaaa tcacggcgcg tgggaaatgt
    27121 ccgctgattg tcggcggcac aatgatgtat ttccgcgccc tgacccaagg tttgaacgat
    27181 ttgcccgaag ccgatgcctg cctgcgtgcc gacttggacg aacaaaaaca gatgtacggt
    27241 ttggattttc tctaccgcac cctgcaaaaa gtcgaccctg aaacagcatg ccgtctgaaa
    27301 ccgaacgaca gccagcgcat cggacgcgcc cttgaagtct attacctgac cggcaggccg
    27361 atgagcgcgc acctgaacgg acagcccgaa cacaccctgc ccttcgaact ctacaccgcc
    27421 gccctgattc ccgaaaaccg cgcccgactg cacgaaaaca tcgccctgcg tttccacctg
    27481 atgcttgaac agggctttat cggcgaagta gaaaacctgc gccgccgcta tcccggcctg
    27541 accgccgact cccccgccat ccgctgcgtc ggctaccgtc aggcgtggga acacctcgac
    27601 ggtgcaaccg acagacaaac cttcatcgaa aaaggcattg ccgccacgcg ccaacttgcc
    27661 aaacgccaac tgacttggtt acgcaaaaca cctttagact gcgttgccga cccgttttca
    27721 gacggcactt caggcacgcg cctgattgag gcggcaaaac ggtttttcgg cgaataaacc
    27781 atgctggcca cgccgccgct cgcccccaaa accgtcgccg ccctgcacag gctcggtatc
    27841 cgtacgctcg aagaactgcg gcaaaacggt tcggtcaaag cctttctgct gctcaaagca
    27901 tcgggtttga ccctcacgaa aagcacgctg tggcaactcg agtcgctgct gaacggcacg
    27961 ccgccgcaag aaatgtccca agcgcataaa gactgcctgc ttgccgagtt gaaaaaccat
    28021 ccgcccgtcg ccgcctttcc gccgcaggaa gaaatgggat acttcatgcg cgaagcactg
    28081 cgccaagcag aacaatccgc cgccgacggc gaaatacccg tcggggcagt catcgtttca
    28141 gacggcaaaa tcatcacatc ggcacacaac acctgcattg ccgactgcaa tgtcagccga
    28201 cacgccgaaa tcaacgcctt ggcacaggca ggcagcgaaa tgcaaaacta ccgccttgac
    28261 ggatgcgaca tctacatcac cctcgaaccc tgcgccatgt gcgcgtccgc actgatacag
    28321 gcacgaatca ggcgcgtgat ctacggtgcg gcagaaccca aaaccggcgc ggcaggcagc
    28381 atcgtcaacc tgtttgccga caaacgcctc aatacgcaca ccgccatacg gggcggaatc
    28441 ctgcaagaag aatgccgcgc cgtattaagc cgttttttcc aaaacaaaag aaaaggttga
    28501 acccatgacc gccctactcg tcatcctcgc cctcgccctg atagccgtcg gcacggcagg
    28561 catcgtctat cccgccctgc ccggcttggc attgatgttt gccggaacat ggctgcttgc
    28621 ctatgccggc ggctatcaaa tctacggcgc aggcatcttg tggacggtcg gactcatcag
    28681 ccttggcggc atactggcgg actatatggc aggcatgttg ggggtaaaat acactggggc
    28741 aggcaaactc gccgtccgag gtgcattggc cggcagcatc atcggcatat ttttctccct
    28801 gcccgggctg atactcggcc cctttatcgg cgcggcggca ggcgaactga tcgatcggcg
    28861 caatatgctt caggcaggta aagcgggctt gggtacgctg ttggggcttg tcgtcggcac
    28921 ggcgttcaaa atcggctgcg ccgtatccat cttgcttatc ctgttggtga aatacatcgc
    28981 ctacctgttt taaccccttc agacggcata ccggcaaatt gctataatgc cgtctgaaat
    29041 ttccgacaaa gcaccattat ggcaaaccaa agacccatct acggcttcca cgccgtcaac
    29101 gcccgattgt ggcaaaaccc caaatcgatt accgaactct atatccaaga aggcaaatcc
    29161 gacgcacgca cgcgcgaagt gctggaaaag gcggcaaacg aaaacatccg cgtatatttt
    29221 gccgatgccg accgcctcaa cgccatcagc aaaggcgcgc gccatcaggg cgtggtcgga
    29281 tttatcgatg cctccaaaaa ccacgtccac ctcgaagacg tattggaaaa cctcagcgaa
    29341 ccgccgctgc tgctgatact cgacggcatc accgatccgc acaacctcgg cgcgtgcctg
    29401 cgtaccgccg acgcaatggg cgtacacgcc gtcatcgcgc cgaaagacaa aagcgcggga
    29461 ctgaacgcca ccgtcagcaa agtcgcctgc ggcgcggcgg aaaccgtccc ctacatcacc
    29521 gtaaccaacc tcgcccgcac cctgcgcgaa ttgaaagaat acggcatctg gattatcggc
    29581 accgacatag gcggcaacgc cgacctttac cattgcaatc tgcccgacag cgcggcatgg
    29641 gtgatgggca acgaaggcga cggtatgcgc cgcctcacgc gcgaacattg cgacatgctg
    29701 gtgtccatac ccatgttcgg cacggtcgaa agcatgaacg tctccgtgtc cgcaggaatg
    29761 gtattaagcg aaacccgccg gcaacgcgta ttgaaaaaca aaaaagcgta agcctcaaaa
    29821 acaatgccgt ctgaaacagg gattccgttt cagacggcat tcgttttcaa aatcagtgtt
    29881 ccaaatggtc ggtatcaaac ttgaccgccg cctcggtttt atcttccggc aagaccaaat
    29941 tcagcaagac tgccgtaatg ccgccgccgg aaatagagtt ttggaacaag acgggcaggt
    30001 ttttaaacac ttccggctca aaccccacgc ccaagcccaa accgaccaac gttgccgcaa
    30061 tcaccgcctc gcgcctgcgg atgccgtggc cgaccaaaat ccgcacgccc gcaatcgcaa
    30121 tcaggccgaa catcaaaacc atcgcgccgc ccaacaccgg actcggaatc gtcgtaaacg
    30181 cgcgtccgac aacggggaac agacccaaca gcaccaaaat cacggcaata tatttgccca
    30241 cgtggcgcga agccactccg gtcatctgaa tcacgccgtt gttttgcgca aacgtcgtca
    30301 gcggcagcga acccaaagcc gtcgcaatca ccgataccaa gccgtcagcc aacacgccgc
    30361 cgcgcaggcg tttggtgtat tcttcgcctt caatcggctg gtcggacacc attgccgttg
    30421 ccgtcaaatc gccgaccgcc tcaaacacgc ccaacaagaa aatcgcaccc gccgcaataa
    30481 atgcgtgcca gtcaaaagca aaaccatatt taaacggtac gggcagcgta accggcggca
    30541 ggttttgcag tgcggaaaaa tccaccttgc ccaaaaacag cgcgacaatg tagccggcaa
    30601 tcagaccgac cgcaatgccg ctcatccgca gcagcggatt tttcatgcag ttgaacacca
    30661 gcacaatcag cagcaccagc gaagccagcc ccaagttttc catcgagccg aacgtgccgt
    30721 ccgccttcgc gccgaagccg ccgccgaaat cggcaatacc gacgtgtacc aaactcagac
    30781 caatcagcat cacaaccacg ccgctgaccg tcggcgtaat cacttttttc aaatacggca
    30841 gaagccacgc cgagaaacac accaaaaacg caccggcaaa cgatacgccc aagagcgtcg
    30901 aaaccatcgc cccctcactc aaaccgccct ctttcatccc cgcgccgagc gcaatcatga
    30961 cggtaacgct ggatggacag catccccgaa ccgaccggcc cgaagcggtt gacctgcaaa
    31021 taagtgccga cacccgacgc aaccatcgcc atcgacacga gatacgccgt catttccacc
    31081 ggtagttcca gcgcgccgcc cacaatcagc gcgggcgtaa tcatcggcac gaaaatcgcc
    31141 aaaagatggg taaccgcgct caagagcgca ttaccgaacg gcggcctgtc ttccaaaccg
    31201 tacaccaaat caggcgaatc cgcctgtttt ttcattgttt cagccattgg agtctttcta
    31261 tccgttgcca aacgttaaaa aattttaaga acgaaattgt aacgaatcaa agattcctta
    31321 tcaattcaaa tcctcatgtg caaaaacacc accggttgtc cgaattcaaa tttctttgaa
    31381 tttccgcacc ggacagtcct tcatttccag agagccttca tgcgcttaac acattttaat
    31441 cctcaacggc gcatccactg cttaaaccca ataatttcat gattataaaa aaaatattat
    31501 ttattaagat atgatgggac ttaagccttg gaaccaaccg tgcggccgat atgtttcaca
    31561 caaacaacaa agtaagctac aatccgaagc ataatcgacc tcactcaatg aataaaaggt
    31621 ttccgtatgt tacaaggtag cttggttgcc ctgattaccc cgatgaatca agacggcagc
    31681 atccattacg accaactccg ccaattaatc gactggcaca tcgaaaacgg cacggacggc
    31741 atcgtcgctg ccggcacgac aggcgaatcc gccaccctct ccgtcgaaga acatttaagc
    31801 gtaatcgaag aaacggtcaa acacgttgcc aaacgcgtcc ccgtcatcgc cggcacaggg
    31861 gcgaacaaca ccgtcgaagc catcgccctt tcccaagccg ccgaaaaagc cggcgcggac
    31921 tacaccctct ccgtcgtccc ctattacaac aaaccttccc aagaaggcat gtaccgccat
    31981 ttcaaagccg ttgccgaagc cgccgcggtt ccgatgatac tctacaacgt gcccggcaga
    32041 accgtcgtca gcatgaacaa cgaaaccatc ctgcgccttg ccgaaatccc caacatcgtc
    32101 ggcgttaaag aagcaagcgg caacgtcggc agcaacatcg aactcatcaa ccgcgcgccc
    32161 gaaggcttcg tcgtcctttc cggcgacgac cacaccgccc tgccgttcat gctctgcggc
    32221 ggacacggcg ttattaccgt agccgccaac gccgcgccca agctctttgc cgatatgtgc
    32281 cgtgccgcgc tgcaaggcaa cattgccctt gcacgcgaac tcaacgaccg gctgattccg
    32341 atttacgaca ccatgttctg cgaacccagt cccgccgcac cgaaatgggc ggtatccgcc
    32401 ttgggcagat gcggaccgca cgtccgcctg ccgctcgttc cgctgacgga aggcggacag
    32461 gcaaaagtcc gcgccgccct gaaagcctca ggacaacttt aaatatagcc atgccgtccg
    32521 aaccgttcgg acggcataac atgacaaaca ctttaatatc catcacacag gatgacacga
    32581 tgacccatat caaacccgtc attgccgcgc tcgcactcat cgggcttgcc gcctgctccg
    32641 gcagcaaaac cgaacagccc aagctcgact accaaagccg gtcgcaccgc ctgatcaaac
    32701 tcgaagtccc gcctgatttg aacaaccccg accaaggcaa cctctaccgc ctgcctgccg
    32761 gttcgggagc cgtccgcgcc agcgatttgg aaaaacgccg cacacccgcc gtccaacagc
    32821 cagccgatgc cgaagtattg aaaagcgtca aaggcgtccg cctcgagcgc gacggcagcc
    32881 aacgctggct tgtcgttgac ggcaaatccc ccgccgaaat ctggccgctt ctgaaagcct
    32941 tttggcagga aaacggcttc gacatcgaat ccgaagaacc cgccatcgga caaatggaaa
    33001 ccgagtgggc ggaaaaccgt gccaaaatcc cccaagacag cttgcgccgc ctattcgaca
    33061 cagtcggttt gggcggcatc tactccaccg gcgagcgcga caaattcatc gtccgtatcg
    33121 aacagggcaa aaacggcgtt tccgacatct tcttcgccca caaagcgatg aaagaagtgt
    33181 atggcgacaa aaacaaagac acgaccatgt ggcagccttc cgcttccgac cccaaccttg
    33241 aggccgcttt cctgacgcgc tttatgcaat atttgggcgt tgacggacgg caggcggaaa
    33301 acgcattggc aaaaaaaccg acccttcccg ccgccaacga aatggcgcgt atcgaaggca
    33361 aaagcctgat tgtctttggc gactacggca gaaactggcg gcgcaccggc cttgccctcg
    33421 accgcatcgg actgaccgtc gtcggtcaaa acaccgaacg ccacgccttc ctggttcaaa
    33481 aagccccgaa cgaaagcaat gcagttaccg aacaaaaacc ggggctgttc aaacgcctac
    33541 tgggcaaagg caaagcggag aaacctgccg aacagccgga actgattgtc tatgccgagc
    33601 ctgtcgccga cggttcgcgc atcgtcctgc tcaacaaaga cggcagcgca tatgccggca
    33661 aagacgcatc cgcactgtta ggcaaactcc attccgaact gcgttaaacc gttcaaaaca
    33721 aaagcagctt ccaaatagaa gctgcttttt tgtgcggaac tttttacaac aaacggggca
    33781 aaatatcctc aaccgcctga accaacgcat ccgccaaagg cggatcgaac gcacaatgcc
    33841 ccgcctggac caccctcaat tccgcttcgg gaaaggcttt cgacagctcc cacgcactct
    33901 gcataggcgt acacaaatca taccgcccct gcacgataac ggtcgggata tgccgtattt
    33961 tgccgatatt gttcaaaatc gccttatcac cctgcaacca accgccgttg acaaaataat
    34021 ggttttccaa acgcgcgatt gccagtgagg cataagcatc ttcatccact ccctccggct
    34081 cgaaacgaat cagatagctt tcccaatccg cccaagcctt cgcggcagac aggcacactt
    34141 cttcatcttg atgaaacagc aacccgtgat acgcctcaat cagccggttc cgccgatttt
    34201 cagcaatcgg cgcgacaaat ttttgccatt gttccggata aatccggctc acgccgcccg
    34261 cctcgttcag ccacgccgtt tcagacggcc tgcacaaaaa tatcccgcgc aacaccaatc
    34321 cctttacccg ttcgggatgg gtttgggcat aagccagcga caaagtgctg ccccacgaac
    34381 cgccgaacac cagccatttc ccgataccca gcatttcacg gactttttca atatccgcca
    34441 ccaaatccca agtcgtattg tcttccgcac aagcatacgg gtgcgaacgt ccgcaaccgc
    34501 gctggtcgat gatgacgatg cggaacacat caggatgaaa aaaaccccga cattcaggcg
    34561 aagcccccgc gcccggcccg ccgtgtaaaa aaatgaccgg cacaccgtcg ggattgcccg
    34621 actcttccca ataaatttga tgaatttcag acacctgcaa gtatccgcta tgaaaaggct
    34681 gttttatttc atacatcatc ttctcccata ttgcttctga caatccgaac atcccgacac
    34741 gcgacatccc gcacgaaaca caagccgccc cgaaaggcaa atgccgaaaa atcgcgataa
    34801 aaaccatcaa tcggaaaata ttgcataaac aggcgaaaat atatcattga aaagaaaacc
    34861 catacaatgt ctgacagaaa caagccgctt tgccattgtt tcctaacagt taaaccccgc
    34921 ccttcaaggc ggcggggcag ggcttgattc aaaatggcgc aagcccctgc cctcaaatcc
    34981 aacacgcagg attaaaccat aatagcggct ttcttattat ttcttattga aacaccgccc
    35041 ggaacccgat ataatccgcc cttcaacatc agtgaaaatc
//
LOCUS       DBJGGI010000014        33920 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.014, whole genome shotgun sequence.
ACCESSION   DBJGGI010000014 DBJGGI010000000
VERSION     DBJGGI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33920
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.014"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(90..1400)
                     /locus_tag="ACSIGJ_000691"
     CDS             complement(90..1400)
                     /locus_tag="ACSIGJ_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HHQ1329633.1"
                     /translation="MDISKQTLLDRVFNLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFIGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFVMVVVLGYFRVQGAIIITILTITVIASLMGLN
                     EFHGVVGEVPGIAPTFMQMDFKGLFTVSMVSVIFVFFLVDLFDSTGTLVGVSHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPVFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTGDVPPMVWVVAVLWALKFWYLG"
     gene            complement(1536..2714)
                     /gene="ftsZ"
                     /locus_tag="ACSIGJ_000692"
     CDS             complement(1536..2714)
                     /gene="ftsZ"
                     /locus_tag="ACSIGJ_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HHQ1329634.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRSVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFSYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDPTPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(2833..4077)
                     /gene="ftsA"
                     /locus_tag="ACSIGJ_000693"
     CDS             complement(2833..4077)
                     /gene="ftsA"
                     /locus_tag="ACSIGJ_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HHQ1329635.1"
                     /translation="MEQQKRYISVLDIGTSKVLALIGEVQDDDKINIIGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIELCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGT
                     VQEREGGGGLLARLKRWIENNL"
     gene            complement(4163..4891)
                     /locus_tag="ACSIGJ_000694"
     CDS             complement(4163..4891)
                     /locus_tag="ACSIGJ_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HHQ1329636.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWNVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRHAPDGLPEKESEE"
     gene            complement(4881..5795)
                     /locus_tag="ACSIGJ_000695"
     CDS             complement(4881..5795)
                     /locus_tag="ACSIGJ_000695"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HHQ1329637.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKERGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLYDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKEKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVTGVGFADLCIEILKAAHVG"
     gene            complement(5907..7316)
                     /gene="murC"
                     /locus_tag="ACSIGJ_000696"
     CDS             complement(5907..7316)
                     /gene="murC"
                     /locus_tag="ACSIGJ_000696"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HHQ1329638.1"
                     /translation="MMKNRVSNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLSSLGIQVYPGHTAEHVNGADVVVASTAVKKENPEVVAALERQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCV
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPVAAADSRALARAIRVLGKLEPIYCENVADLPQMLMNVLQDGDVVLNM
                     GAGSINRVPSALLELSKQI"
     gene            complement(7471..8538)
                     /gene="murG"
                     /locus_tag="ACSIGJ_000697"
     CDS             complement(7471..8538)
                     /gene="murG"
                     /locus_tag="ACSIGJ_000697"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HHQ1329639.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRVRGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGIRGNGIKRKLMLPFTLYKTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPEEVRPQMYHQ
                     SGRNKLGNLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(8542..9813)
                     /locus_tag="ACSIGJ_000698"
     CDS             complement(8542..9813)
                     /locus_tag="ACSIGJ_000698"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HHQ1329640.1"
                     /translation="MGDGVHTLLLDRPIVRDGRKFDAPLLWMVVLMTAFGLLMIYSAS
                     VYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRTWRRLVPWIFALSGLLLVA
                     VLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLFTRREEVLRSMESLGWQSI
                     WRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFGLVLIMVQPDFGSFVVITV
                     ITVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQRVVAFLDPWKDPQGAGYQL
                     THSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIAEEFGFFGMCVLIFCYGWL
                     VVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGVNIGALPTKGLTLPLMSYG
                     GSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(9992..11329)
                     /gene="murD"
                     /locus_tag="ACSIGJ_000699"
     CDS             complement(9992..11329)
                     /gene="murD"
                     /locus_tag="ACSIGJ_000699"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HHQ1329641.1"
                     /translation="MTFQNKKTLVAGLGGTGISMIAYLRKNGAEVAAYDAELKAERVA
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAELQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVMNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDVFCRAMKRAGREVKRFSLEHEADFWLERGTGCLKQGNEDLISTQDIPLQGLHN
                     AANVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALKDKAKGVFLIGVDAPQIRRDLDGCGLN
                     LTDCVTLEEAVQTAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(11414..11794)
                     /locus_tag="ACSIGJ_000700"
     CDS             complement(11414..11794)
                     /locus_tag="ACSIGJ_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HHQ1329642.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWAGDVSDFRENLQAAEQGNAAAQ
                     FNLGVMYENGQGVRQDYVQAVQWYRKASEQGDAQAQYNLGLMYYDGRGVRQDLALAQQ
                     WLGKACQNGDQNSCDNDQRLKAGY"
     gene            complement(11919..13001)
                     /gene="mraY"
                     /locus_tag="ACSIGJ_000701"
     CDS             complement(11919..13001)
                     /gene="mraY"
                     /locus_tag="ACSIGJ_000701"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HHQ1329643.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAVIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLAAFPVVLVAAGLAIFAYV
                     SGHYQFSQYLQLPYVAGANEVAIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            complement(13246..13422)
                     /locus_tag="ACSIGJ_000702"
     CDS             complement(13246..13422)
                     /locus_tag="ACSIGJ_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329644.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVPAR"
     gene            complement(13425..14783)
                     /locus_tag="ACSIGJ_000703"
     CDS             complement(13425..14783)
                     /locus_tag="ACSIGJ_000703"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HHQ1329645.1"
                     /translation="MKPLDLNFICQALKLPMPSENKPVSRIVTDSRDIREGDVFFALA
                     GGRFDAHDFVGGVLSAGAAAVVVSREDCAALGGALKVDDTLAALQTLAKAWRDNVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDDAVSATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNALRAHVGCGFDGVGDIAKAKSEIYAGLCSDG
                     MALIPQEDANMAVFKTATFNLNTCTFGVDSGDVRAENIVLKPLSCEFDLVCGDERTAV
                     VLPVPGRHNVHNAAAAAALALAAGLSLNDVAEGLQGFSNIKGRLNVKAGIKGATLIDD
                     TYNANPDSMKAAVDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLSHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(14908..16554)
                     /locus_tag="ACSIGJ_000704"
     CDS             complement(14908..16554)
                     /locus_tag="ACSIGJ_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HHQ1329646.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWKYCVSVGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMEKIAGDKDWREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSRTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLTGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGEWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVPGEGNNLAFIDRQSDDPAPWYAGKGKSAKNTSKK"
     gene            complement(16577..18055)
                     /locus_tag="ACSIGJ_000705"
     CDS             complement(16577..18055)
                     /locus_tag="ACSIGJ_000705"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HHQ1329647.1"
                     /translation="MFSKLTPLAETGILPLPCANAAGRLLHSDSRQIKQGDIFVACQG
                     EYTDGRSYIPAAIANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGMLAAQVYGN
                     VSGGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIIGTVGNGFWGALEETAHTTPAP
                     VDVQTLLYRFRQQGATAAAMEVSSHGLDQSRVNGVPFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINTDDGYGAELAGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIEAVFRTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALSTLQEIKPQGAALWCVFGCGGNRDCG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPQDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(18080..19828)
                     /locus_tag="ACSIGJ_000706"
     CDS             complement(18080..19828)
                     /locus_tag="ACSIGJ_000706"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HHQ1329648.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAMAVLFACLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQALPATRGTVSDRNGAVLALSAPTESLFAVPKDM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLYGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPQNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVR
                     MHSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTVLTHDGELLPVSF
                     EKQAVAPKGKRVIKASTAKKVRELMVSVTEAGGTGTAGAVDGFDVGAKTGTARKLVNG
                     RYVDNKHVATFIGFAPAKNPRVIVAVTIDEPTANGYYGGVVTGPPFKKIMGGSLNILG
                     VSPTKPLTAAAVKTPS"
     gene            complement(19889..20152)
                     /gene="ftsL"
                     /locus_tag="ACSIGJ_000707"
     CDS             complement(19889..20152)
                     /gene="ftsL"
                     /locus_tag="ACSIGJ_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HHQ1329649.1"
                     /translation="MNKSNFFLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRVQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(20179..21135)
                     /gene="rsmH"
                     /locus_tag="ACSIGJ_000708"
     CDS             complement(20179..21135)
                     /gene="rsmH"
                     /locus_tag="ACSIGJ_000708"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HHQ1329650.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIVRAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAIRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHEPLPSWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPE"
     gene            complement(21132..21587)
                     /gene="mraZ"
                     /locus_tag="ACSIGJ_000709"
     CDS             complement(21132..21587)
                     /gene="mraZ"
                     /locus_tag="ACSIGJ_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HHQ1329651.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVATLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            21866..23113
                     /locus_tag="ACSIGJ_000710"
     CDS             21866..23113
                     /locus_tag="ACSIGJ_000710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHQ1329652.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTFIMLLPSPEARQSSLNLQTLIAE
                     EIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTPLFAQTLARQL
                     QELLPQTAGNIYEFGAGTGQLAADLLGSVSDSINCYYIIEISPELAARQKNLIQARAP
                     EASQKVVHLTALPEAFDGIIIGNELLDAIPVEIVRKNEGGLLEHIGVCTDNGRFAYSA
                     RPLHDPSLSTSASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDAAQ
                     YYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLTGYLPQ
                     SHFLLNLGITELLAQTGKTDSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGIDWA
                     GFRFGDICHKLQPSCRLNPLQTA"
     gene            23209..23285
                     /locus_tag="ACSIGJ_000711"
     tRNA            23209..23285
                     /locus_tag="ACSIGJ_000711"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23243..23245,aa:Met,seq:cat)
     gene            23293..23368
                     /locus_tag="ACSIGJ_000712"
     tRNA            23293..23368
                     /locus_tag="ACSIGJ_000712"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23326..23328,aa:Ala,seq:ggc)
     gene            complement(23554..24375)
                     /locus_tag="ACSIGJ_000713"
     CDS             complement(23554..24375)
                     /locus_tag="ACSIGJ_000713"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HHQ1329653.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFDK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTVMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(24408..25052)
                     /locus_tag="ACSIGJ_000714"
     CDS             complement(24408..25052)
                     /locus_tag="ACSIGJ_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HHQ1329654.1"
                     /translation="MKFKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQPGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDTYLRTEHVVWRPEMLGLARMAAAVKLSGL
                     KYQANSAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAVALKMQK
                     LTEQYGIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(25055..25918)
                     /gene="ftsN"
                     /locus_tag="ACSIGJ_000715"
     CDS             complement(25055..25918)
                     /gene="ftsN"
                     /locus_tag="ACSIGJ_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HHQ1329655.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILLYLNQGGQNAFKIPA
                     PSKQPAETEILKLKNQPKEDIQPEPADQNALSEPDVAKEAEQSDAEKAADKQPVADKA
                     DEVEEKAGEPEREEPDGQAVRKKALTEEREQTVREKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAAKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTFGKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSEVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHG
                     VASLIRAIEGK"
     gene            complement(26024..27520)
                     /locus_tag="ACSIGJ_000716"
     CDS             complement(26024..27520)
                     /locus_tag="ACSIGJ_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HHQ1329656.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHTARFALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLAEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARGKQYARQGKVNAALSVSELDSQARIQKEAQEAL
                     GSLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(27534..27854)
                     /locus_tag="ACSIGJ_000717"
     CDS             complement(27534..27854)
                     /locus_tag="ACSIGJ_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HHQ1329657.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            28342..29868
                     /gene="putP"
                     /locus_tag="ACSIGJ_000718"
     CDS             28342..29868
                     /gene="putP"
                     /locus_tag="ACSIGJ_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HHQ1329658.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGASTVVVWAEWV
                     KKPARAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSREIQERFEKADADYRAAR
                     "
     gene            30120..33725
                     /gene="putA"
                     /locus_tag="ACSIGJ_000719"
     CDS             30120..33725
                     /gene="putA"
                     /locus_tag="ACSIGJ_000719"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HHQ1329659.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALGRLIGKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDRLIKSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHGDVVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLILGAGD
                     TGAALTNDASIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKAAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALIHKLPFNAEEKKAAAAALGHARIRTLRRAESVLTGPTGERNSISWHAPKRVWVHG
                     GSLLQAFCALTELAAAGIRTVVEPGSPLASYSADLDGLLQISGKPENTGVNHVAALSP
                     LDSSRKQELANRDGALIRILPSGQGLDILQVFEEISCSINTTAAGGNAGLMAVAD"
BASE COUNT         7977 a         9766 c         8400 g         7777 t
ORIGIN      
        1 ttttatatta tttatttaaa attaaatata tctggcaaac aaaatgccgt ctgaaaacct
       61 ttcagacggc atttttaata tcgaatcaat cagcccaaat accagaattt caatgcccac
      121 aatacggcaa caacccatac cataggcggc acgtccccag tccggcgaca caaaagtttg
      181 accacggcat agctgatgaa gccgaaggcg atgccgtctg caatcgaata ggtaaacggc
      241 atgaagacaa tggtcaggaa cgcgggcgcg gcttcagtca tatcgtccca atcaatgtcc
      301 ctcgcactgc ggagcatctg cgtgccgaca taaagcagtg cgggcgcggt ggcaaatacc
      361 ggaacacttt tcgccaatgg ggagaacatc agacacgcca gcatcaatac gccgacggta
      421 accgccgtca ggccggtccg tccgcctgcc gatacgcccg ccgcgctttc cacataaggc
      481 gtggttgaag aagtacccaa agccgcacct gccacaatgg cggtagagtc tgcaagcagt
      541 gcgcgtttca ggcggggcag cttaccgtcc accagcagtc cggcacggtg ggatacgccg
      601 accagcgttc cggtactgtc aaacaaatcg accaagaaga agacgaaaat cacgctgacc
      661 atgctgacgg taaacagacc tttaaaatcc atctgcataa aggtcggcgc aatgcccggt
      721 acttcgccga ccacgccgtg aaactcgttc aaacccatca ggctggcaat gacggtaatc
      781 gtcagaatgg tgatgatgat tgcgccttga acgcggaaat accccaatac gaccaccatg
      841 acaaaaccga acaatgccaa cagtgcgctg ggctgatgaa tatcgcccaa gccgaccaag
      901 gttgccggat tggcaacgat aatgcctgcg cctttcaggg aaatcagtgc caaaaacaaa
      961 ccgataccgg cggcaatcga cattttcaaa cccataggca gtgcgttgac cagcatttcc
     1021 ctgactttaa aaaagctgaa caggatgaaa atcagaccgg aaatgaacac cgcacccaac
     1081 gccacctgcc aaggcacgcc cataccctta acgacggcaa aggtgaaata ggcattcagc
     1141 cccatccccg gggcaagcgc aatcggatag ttgccgataa aacccatgac aaaacagccg
     1201 atggcggatg cgatacaggt ggcgacgaat accgccccca tatccattcc ggtctcgccc
     1261 aaaatcaggg gattgacgat aacgatgtag cacatcgtca aaaaggtcgt caaacccgcc
     1321 atcaactcgg tacgtaccgt cgtaccgttt gccttcaggt taaaaaccct atccagcaat
     1381 gtttgttttg aaatatccat atctagcagt ccgtttcaag taaaaaatct gaacccgcac
     1441 accgcaaacc gcacaggatt cggcatattg aaaaatatac ctcccaaatc gggaaaaccg
     1501 gttcgggagg caataagttt acggcagacg gcacattatt tgtctgaatt gtgttgacga
     1561 cgcaaaattg caggaatttc aaaatcgtca aggacagact ggttatcgaa atccgctgct
     1621 gtcagattca tggtgcggat accgcgattg gtgcggatca taccttcgac attgtggctt
     1681 tgctcctgtt tggacggggc aaccgcttct acttcccttg ccggagtagg atcgaccgct
     1741 cctttttctt tcagaccggt agcgataatg gtaatccgga tggcatcttc gctcatggtc
     1801 tcgtcttcag cggcaccgaa tttgcattcc aaatcgggat gcgcgctttg gttgacgatt
     1861 ttcatgactt cagacaactc ggacattttc aggcaacccg gagcagtcgt aatattgacc
     1921 agcacaccgc gtgcaccatc caaagttaca tcgtccagca gcggactgga aatggcctgg
     1981 tcggtcgcca tacgcgcacg gtcgatacct tgggcataac ccgaacccat catagcgata
     2041 ccgcggttgc tcatcacggt tttcacgtcg gcaaagtcga ggttgatgat ttcgctcggg
     2101 caagttacca cttcggaaat gcctgcgacc gcatcgcgca atacattgtc cgccgcgcgg
     2161 aaggcttcgc gcatagttac gtcttcaccc aatgcggtca tcagtttgtc gttcgggatg
     2221 ataatcagcg aatcgacgtg ttctttcaac tgttccaacc ctgcctgtgc cacatggacg
     2281 cgtttgcctt cataagaaaa cggacgggta actacggcaa cggtcagaat gcccaaagac
     2341 ttggcaatct cagcaacaac cggcgcggaa ccggtaccgg taccgccgcc cataccggtc
     2401 gtgataaaca gcatattggc accgcgaatg gcttcttcaa tggcctcccg atcttcctgt
     2461 gctgccgcac ggccgatatc gggattcgca cccgcgccca aaccgcgtgt cagattcgta
     2521 cccaactgga ttctcttcgc cgcatggttt tttgccaaag actgcgcatc tgtattggca
     2581 ctgataaact ccacactgcg cacattgttg gcaaccatgt tattgattgc attgcaaccg
     2641 ccgccgccca agccgattac tttaattacc gcagggctga ctgccgattc tgccacgtcg
     2701 taaacaaatt ccattcaaaa actcctgatc gccccgttca gaggacggtt taaataaatt
     2761 attatttatt atataagaag tatcttactg ctggcaaaat acttctcacc tgtcaaacgg
     2821 caatccaact gttcagaggt tgttttcaat ccaccgcttc aatcttgcca acaaaccgcc
     2881 gcccccttct ctctcttgca ctgtaccgtt ttccggttgc ggcaagtttc cttccagctt
     2941 gcatgctgca tgaagcagcc cgatagcggt agaaaagcgc ggtgtgcgga cacggtcgga
     3001 caaaccgccc atttcttggg gcgcaccggt gcgtacaggc aaatcgaaga ttttttcggc
     3061 aaattccaca atcccggtca tcatggacac accgccggtc agaacgatac ccgcattcag
     3121 cacttctttg gggaaacccg atttttgcag ttcgcccagc actacgccaa aaatctcctg
     3181 aatacgtgcg ctgatgattg ctgccaaaac cttactggaa acctgacgcg atgtccggtc
     3241 acccacgccc ggaacttcaa tcatctcgcc caagccttcc gtatcgcatg atgccacgcc
     3301 ataatggatt ttaatgtact cggcggcatc gagaggtgtt ctcaacgatt tggacaaatc
     3361 tttggtaatc agattaccac cggccggaat gacggacgta tggcggatgg cgccgttcat
     3421 ataaacggca atatcggtcg ttccgccacc aatgtcaatg acgcatacgc cgaggtcttt
     3481 ttcatcttca gtcagcaccg cctgcccgct tgccaacggt tgaagcatga tctgatcgct
     3541 tttcaagccg cacagctcga tacatttttg gacattctgc actgccgtac ttgcaccggt
     3601 aatgatgtgc acccgcgtat ccagacgcac accgctcata ccgatgggct cccttacacc
     3661 aagttgggtg tcgataatgt agtcttgaac cacggcatcg agaatttttt gatcgggcgg
     3721 gatattgatt gcctttgccg tttcaatggc gcggtcgatg tctgcctgtg ttacttctcc
     3781 gtctttgatt ttaaccacac cttgcgaatt aaggctgcgg atgtggttgc ctgcgatacc
     3841 tgtggtaacg tgggtaattt tggtatccgc catcagctcg gcatcattga ccgcctgcct
     3901 gatggcttgg acggtggcat cgatattggt taccatgccc gcgcgcaagc cccgtgaagg
     3961 agcctgcccc aaaccgatga tgttgatttt gtcgtcatct tgaacttccc cgatcagtgc
     4021 gaggacttta gacgtaccga tatccagtac gctgatgtat cttttctgct gttccattgt
     4081 tcgtctgccc ttaaaactga ttgaaatttg cgtcgcaccg tttcagacgg cacggccgta
     4141 atatgtccga tacctgttcc cactattctt cggattcttt ttcgggtaaa ccgtcgggag
     4201 catggcggac tgaaaatccg tccttatacc tcatatccac ataggataac cgatttttat
     4261 tcttacgcaa cagatgctgc cacgcttcgg taaaaagccg gaggcgtttc atctcgtttt
     4321 cccgtccgag cctgacggtg atgccgttgt ccaaaacgac attccacgcc gaacgtgccg
     4381 tataggtcat ctctttgatg cccaaaccct gttttgccaa aacagtcgaa aattcgtcat
     4441 aacggcggag catttcggca gacgttcctt ccgcgcctct gaataccggc attccgggtc
     4501 tgtccaagcg ggcttcaaaa acattgcctt cgccgtccac caaggcatgg tcgccccaac
     4561 gtgcaaccgg cttgcgctcg gtcaggacga cctcaaccgt atcgggaaaa cggcggcgca
     4621 ccatgaccga cgcaatccac ggataccggc ggtaggcttc ctgtgcgcca ttgatgtccg
     4681 tcctcaaaat attcccatgg atgtattctt tcgccaaact gcccaatgcc ttcttatcgg
     4741 aataaaccag gttgcccttc agcgacacct gcttgacggg cagatgattc gaattgtaaa
     4801 accaaaccag cccggacgca gcaagcagca tcgccatcat gacaagcagc cagcgcgtca
     4861 gccgttccat cgcttcggca ttatcccaca tgtgcggcct tcaaaatttc aatacataaa
     4921 tcggcaaaac ccacgcccgt aacggcagcg gatttcggta ctaaactatg gccggtcata
     4981 ccgggcaggg tgttgatttc caacagatag agtttgccgt cggtatcttt gaggaaatcg
     5041 acgcgcacgc agccttccgc accgattgcc tgtgcgccgc gaaccgccag ttcgcgcatc
     5101 aggctttctt cggcttcggt caaatcttcc gaaggacatt gataaatggt gtcgtctcgg
     5161 ttgtacttgg cttcgtagtc gtaaaactcg gttgcgggga tgatgtgtat gccgggcagc
     5221 cctttgccgt tcaggacggg gcaggaatat tcgccgccgc cgataaaacg ttcggcaatg
     5281 atttcgccct gaaggtgttt caattcttcg taaacgcttt tcagacggcc tttttctttg
     5341 acttttacca cgccgacgct gctgccttcg gccgccggct tcacaaacat cggcagaccc
     5401 aatttttctt cgacggcatc gaaatcggta tcatcgtaca gtacggcgaa ctcgggaacg
     5461 ggtaatccca atgcctgcca aatcagtttg cagcggtatt tgtccatgcc gatggcggag
     5521 gcggcgacac cgctgccggt atagggaatg cccaacagtt ccaatgcacc ctgaacagcc
     5581 ccgtcttcgc cgtaagtacc gtgaaggatg ttgaatgccg tctgaaagcc ccgctccttc
     5641 agttcggata acggcgtttc cttagggtcg aaggcgtatg cgtctatgcc tttgcttttt
     5701 aaggcgttca aaatggcggt accgctgtcc agcgagattt ctcgttcgct ggaaaaacca
     5761 cccatcaata cggccacttt gccaaaattc tgcattgttt ttgttctttc ctaattgctt
     5821 tatgcttgtt gtcagaggtc gtctgaaacc tgattgcggt ttcagacgac ctttatatga
     5881 tgttccgtct gtcaggcggg tgtatctcaa atctgtttcg acaattccaa cagcgcggag
     5941 ggaacgcggt tgatgcttcc cgcacccata ttcaacacaa catcgccatc ctgtaaaaca
     6001 ttcatcagca tttgcggcag gtcggcgacg ttttcgcagt aaatcggctc aagtttgccc
     6061 aatacgcgga tagcacgcgc caaggcgcgg gagtcggcgg cggcaaccgg ctcttcgccg
     6121 gcggcataaa cttcggtcag taccagcgca tcaacggtat tgagtacttt ggtaaagtct
     6181 tcaaacaaat cgcgcgtgcg ggtatagcgg tgcggctgga aggcgagtac caaacgtttt
     6241 tccggatacg cgccgcgtgc agcggcaagg gttgccgcca tttcgacggg gtggtgtccg
     6301 taatcgtcca ccagcaaagc ggtcccgccg tttggcaact tgatgtcgcc gtatttttgg
     6361 aagcggcggc cgacgccttc aaagccgagc aagccttttt ggatcgcttc aaccgatgcg
     6421 ccgacttcca gcgccacgcc gatggctgcc aatgcgttca gcacgttgtg tctgccgggc
     6481 atattcagca cgacttcaaa cgacccctgc tcatgtcctt tcatttgaac atggacggtg
     6541 aatttcattt gcgcgccgac gttttcgatg tcggtggcgt agatgtcggc ggtatcgtcc
     6601 aaaccgtaag tagcataagg tttgctcact ttgggcaaaa tcgcgcggac gtgttcgctg
     6661 tcaacacaca aaaaggcttt gccgtagaag ggcatacggt ggatgaaatc gataaacgcc
     6721 tgatgcagtt tttcgacgct gtgcccgtag gtatccatat ggtcttcgtc gatattggta
     6781 acgacggaca taatcggggt cagatgtagg aaagaggcat cggattcgtc ggcttcggca
     6841 acgatgtatt cgcctttgcc caagcgggcg ttggtgcctg cggcgttgag tttgccgccg
     6901 ataacgaaag tggggtcgag tcctgccgcg ccgaggatgg aggcggtcag gctggtggtc
     6961 gtggttttgc cgtgcgtacc ggcaatggcg atgccgtcac ggaagcgcat cagctctgcc
     7021 agcatcaagg cgcgcggaat aacgggaatt tgccgctcca acgcagcgac aacttcggga
     7081 ttttctttct tgacggcggt agaggcaacg acgacatccg caccgttaac gtgttctgcg
     7141 gtatggccgg gataaacttg aatgcccagg ctgctcaaat gctcggtagc ggcatttcgc
     7201 gcctgatccg aaccggaaac tttaaagccc aaattgtgca agacttcggc gataccgctc
     7261 atgccgacgc cgccgatacc gacaaaatgg atgttgctta ctcgattttt catcataatg
     7321 ttgcgttccg gttggttttt agtgcgtaaa ggcgttattt taaaggtctg accgtttgcg
     7381 caccatagtt ttctgacaaa tatatagcgg attgaaataa aaaacatcca tgccgtctga
     7441 acggcttttc agacggcatg gttcggcagt ttacgccgca cacgcaatcg cggcttcggc
     7501 aacgtcatcc gcgctgtgcg gcaatgccaa cgtacgggcg ttttccgccc atttgaggca
     7561 tttttcgcgg ttgaggctgc cgaggatttc ggcgagtttt tccgccgtca actgggtttg
     7621 cggcaacagc agccccgctt ctgcctgcac catgaaacgc gcgttggcgg tttgatggtc
     7681 atcaacggcg tgaggatacg gcactaacaa cgcgcccagc cccgccgccg tcaactcggc
     7741 aatcgtcagc gcgccggcac ggcaaatcac caaatcggca tcacggtagg cggacaccat
     7801 gtcggtaata aattccacgc attccgcttt cacgcccaac gcgtcataat ccgcctgaag
     7861 attgcccagc ttgttacgcc ccgactggtg gtacatctgc gggcgcacct cttcaggcag
     7921 cagtgccaac gcctgcggta cggttttgtt caaaacgtcc gcacccaaac tgccgccgac
     7981 caccaaaatt ttcagacggc cttcgcgccc ttggaagcgt tcggcaggca cgggcaggtt
     8041 gctaatatcg gcgcggacgg ggttgccgac caaaccgcct tcgtggctga acgctttcgg
     8101 aaaagcgtac aacacccgtt tcgcccagcg cgacaggtgg cggttggaca agcctgccac
     8161 ggcgttttgc tcgtgaatca caatcggcac gcccaagagt ttcgccgcca gaccgccggg
     8221 aaaggtaaca aaaccgccga agccgatgac gcactcgaca cggtgtttgc ggataatccg
     8281 ctgcgcttcg cggacggttt tgtacagagt aaacggaagc atcagcttgc gtttgatgcc
     8341 gttgccgcgt attcctttaa tcgccagcgt ttccaagcgt atgccgtatt gcggcacgat
     8401 gcgctcttcc atcgaatcct tgctgcccag ccaaattaca tgatgaccgc gcacgcgcaa
     8461 tgaatccgcc acagccagag ctgggaaaat gtgtccgccc gttccgcccg ccatcagcat
     8521 aaaggtttta ccgcccatga tttactccac ccggtaaccg cgcattttcc ggcggttttc
     8581 ataatcgata cgcaacagca gcatcatgct gatcagcatg aaaaagactg acgaaccgcc
     8641 ataggacatc aacggcagcg tcagaccttt ggtcggcaaa gcaccgatgt tcacaccgat
     8701 attgaagaaa ctttggatac cgatccaaat gccgataccc gaagcgatat aggcgttgaa
     8761 agtcaaaccc aaatcgcgcg actgcttgcc gatggaaaac gcccgcacca ccagccagcc
     8821 gtaacagaat atcagcacgc acatcccgaa gaagccgaat tcttcagcga tgatggcaaa
     8881 aataaaatcg gtatgcgctt ccggcagaaa gccgcgtttg ctcaaactcg cacccaaacc
     8941 cataccgaac cactctccgc gcccgattgc catcagagag tgggtaagct ggtagccggc
     9001 accctgcggg tctttccacg ggtccaaaaa tgccactacc cgctgcacac ggtagggagc
     9061 ggcggtaatc atcagcacca tcccacccaa gacgctgcct accaggacaa aaaaatattt
     9121 ccacggcaat cctgccagaa acagcattcc aacggtaatg acggtaatga cgacaaacga
     9181 accgaaatcc ggctgtacca ttatcagcac caaaccgaac gccaccagca taatcggcag
     9241 gatgatcgcc cggaaacggc cgtacatttc taatgtttca cgacgtgcct gcggattggt
     9301 ggcggacata atcaggttgg ccgtcccccg ccaaatcgac tgccaaccca aactttccat
     9361 gctgcgcaac acttcttcac ggcgcgtgaa caggcttgcc aaataaagga tgactgccag
     9421 cttgaacagc tcggtcggct ggaaattcaa cggacccaaa ggtatccaac gggtcgcgcc
     9481 attgatttcg cgcccggcaa tcaatacggc taccagcaac aggccggata aggcaaaaat
     9541 ccacggcaca agccgccgcc atgtcctcat cctgcaaaga aaccataaaa aaccgctcgc
     9601 tataaggccg gcaacgacga accccgcctg cctggtcaaa tagaaaaact gatcgccgcc
     9661 ttccttcgat gccaaataca cagaagccga ataaatcatc agcaggccga acgccgtcat
     9721 cagcaccacc atccacaaaa gcggcgcgtc gaatttcctg ccgtcgcgca caatcggcct
     9781 gtcgagcagc agagtgtgga caccgtcgcc cacttttacc aatacttccg aaatcttcaa
     9841 aaaaccacct gccagtctgt ttgcacctgc cgcaaagggc aaaaatttca gacggcagac
     9901 aatgccgtcc gaacatacga tacatcccaa atcggtattc taaatcttta cttgccgccc
     9961 aacaatgacg gcgtttgtgt ttcggacggc atcacaaagc cttaaacgct tcgataaaca
    10021 cttccgaacg gtgcgcgtag cctttaaaca tatcgaaact cgcgcaggcg gggctgagca
    10081 agacaatatc gcccgcttcg gcttgggcgt atgccgtctg aaccgcctct tccaaagtga
    10141 cgcagtcggt caggttcaag ccgcagccgt ccaaatcgcg gcggatttgc ggcgcatcga
    10201 cgccgatcag gaacacgcct tttgccttat ctttcaacgc gtcgcgcagg ggcgtgaagt
    10261 cctgcccttt gcccatgccg cccaaaatca cgaagagcgg gttttgcaaa ccggcaatcg
    10321 cggcggcggt cgcgccgaca ttcgtgcctt tgctgtcgtc gatgaatacc acgccgtttt
    10381 tctcgccgat tttttccaca cggtgcggca agccttggaa ggttttgacg tgttccagca
    10441 atgcttcgcg cggcaaaccg acggcctcgc acaaagccac ggcagccata acgttggcgg
    10501 cgttgtgcaa accttgcagc gggatgtctt gcgtagaaat caaatcttca ttgccttgtt
    10561 ttaaacagcc cgtcccgcgt tccaaccaaa aatcggcttc gtgttccaag gaaaaccgtt
    10621 tcacttcacg ccctgcccgt ttcatggcgc ggcagaacac gtcgtccgca ttcaaaacct
    10681 gcactccatc gccacggaaa atcttggctt tggtatgcgc gtagtcgagc aaatcgtcgt
    10741 agcggtcgag atggtcttcg gaaatgttca tcaccgtcgc cgcagtcggg cgcaggcttt
    10801 cggtgttttc cagttggaag ctggaaagct ccaacaccca cacgtccgcc tttttgcctt
    10861 cgcgctgcaa ttctgcctcc aaaaccggcg taccgatatt gcccgcaatg acggtatcca
    10921 gcccgcactt gatgcagaga tagccgacca ggctcgttac cgtggttttg ccgttgctgc
    10981 cggtaatcgc aatcaccttg tcgccgcggc ggttcacaat gtccgccagc aattcgatgt
    11041 cgcctagcac gcgcccgccg ttttgcttga acgcctcgat atccggctgc cgctcgctga
    11101 tgccgggact gagcgccaga atatcgaaac cgttgtccaa cgcatctttc agacgacccg
    11161 tgtaaaacac cagcccgtca aacatcttac cgatttgcgc cacgcgctcg gctttcagct
    11221 ccgcatcata agcggcaacc tccgcgccgt ttttgcgcag ataggcaatc atggaaatac
    11281 ccgtgccgcc aagtccggcg acgagggttt ttttgttttg gaaagtcatt ttggttttgt
    11341 cctaaaacaa atcatattgg gcaggagacg tccgcccttg cccaagccgc tttcagacgg
    11401 catcgcgagc cgattaataa cccgccttca ggcgttggtc attgtcgcag ctgttttggt
    11461 ctccgttttg acaagccttg ccaagccatt gttgagcgag cgcaaggtct tggcgcacgc
    11521 cgcgtccatc gtaatacatc aagcccaaat tgtattgggc ttgggcatcc ccttgttctg
    11581 aagccttgcg ataccactgc actgcctgta cataatcttg acgaactcct tgtccatttt
    11641 catacatcac gcccaaattg aattgggctg ctgcatttcc ctgttctgcc gcctgcaagt
    11701 tttcccgaaa atccgatacg tcacccgccc acaccgcttg gttcaagccc aaggcaatca
    11761 gggcggcggc aagccatttg actgtctgtt tcatggtttt ctcctgtttt agtataaggc
    11821 gggtttcagc cgccgttaac gataggacgg ggcggattgc cgccgcaggt ttattgcgcg
    11881 ttcaaatgcc gtctgaaagg tgttcggacg gcataggttc agcgaatttt gagggtactc
    11941 aaaccgatca ataccaagac gatggtaata atccagaaac ggacgacgac ttgggtttct
    12001 ttccagcctt tttgttcgta atggtgatga atcggcgcca tcaggaagat gcgttttttg
    12061 gttttcttgt accagccgac ctgaagcata acggacacgg cttctacgac gaacagaccg
    12121 cccataatga cgaggacaaa ttcttggcgg acgatgacgg caacggtacc gagcgcggca
    12181 cccaatgcca gcgcgccgac atcgcccata aagacttgcg cgggataggc gttgaaccac
    12241 aaaaatccga ggcacgcgcc gcacatggcg gtgcagaata tagcgacttc gttcgcgccg
    12301 gcgacatagg gaagctggag gtattgggaa aattggtagt gtccgctgac gtaggcgaaa
    12361 atggcgagcc cggcggcaac gaggacgacg gggaaggcgg caaggccgtc caagccgtcg
    12421 gtgaggttga cggcgttgga tgtgccgacg atggtcaggt aagacaacac caaaaagccg
    12481 accacgccca gcggcagggc gatttgtttg aaaaacggga caatcaaaat attgttggcg
    12541 gaattggcgg caaggtaaaa caatgccaaa ccggcgataa cggcaacgct tgactgccac
    12601 accattttga atttggcgga cacgccgttg gggtctttat aaacgacttt gcgccagtcg
    12661 tcgtaaaaac cgagcgcacc ggtggcaagc agtacgccca agagaatcca gatatacggg
    12721 ttcgcccagt tgccccacaa cagggtggac acggtaatgg cggtcagaat cagcgaaccg
    12781 cccatcgtcg gcgtgccgtt tttgacgagg tgggtttgcg ggccgtcggt acgcactgcc
    12841 tgcccgcatt tgagcgcggt cagcctgcgt atcgtccacg ggccgaacat cagggaaaac
    12901 gccaaggcgg tcaacgccgc cataacggcg cggaatgtgg tgtattgaaa aatattcaga
    12961 ccggttaacc agttgctgaa atgtgcgagc cataaaaaca tggggcttcc ttttttgttt
    13021 tgtcgttata gcggattaac aaaaaccggt acgtcgttgc cccgccccgg ctcaaaggga
    13081 acggttccct aaggcgccca agcaccgggc gaaccggttc cgtactatcc gtactgtctg
    13141 cggcccgccg ccttgtcctg atttttgtta atccgctata catcgggctt gcgcccgctt
    13201 gaggtttgct tgctgtccgg caaggtttcg gacggctttt gcagattaac gtgcagggac
    13261 tttcccaatc acgcaaatcg ggtagctccc cggatttttg ccgccgccca agtctaaaca
    13321 taaatcttca taaagatacg cctgcgtccg cattccggca taaaacgcgc cgaccagcgc
    13381 ggcaatcagc aataaggctt taaaaaaacg tcggcttttc attttcactt atcctccaat
    13441 gcctcgacca cttcttccat ctgcataaag cgcgaacctt tcaccaacac ggtggcgcgt
    13501 tcgggcaaat cgtggctcaa cacttgaatc aacgggtctt tggcggcgaa ccacaaaccg
    13561 tccgcgccaa atttttccgc cgcttcgacg ctgttgtcgc cgacaaaata agccgcttcg
    13621 atgccttggt cgcgggcgta cgcgccgact tcggcgtgca tggcggcggc ttcgtcctcg
    13681 cccagttcgc ccatatcgcc catcacgaaa atgcgcggcg caggcatacg cgccaacacg
    13741 tcaaccgcgg ctttcatact gtcgggattc gcattataag tatcgtcaat cagggttgcg
    13801 cccttgatgc cggctttgac gttcagacgg cctttgatgt tgctgaagcc ttgcaaacct
    13861 tccgccacat cgttcaaact caaaccggca gccaaagcca gcgcggcggc agcggcggcg
    13921 ttgtggacat tgtggcggcc gggaacaggc agcaccacgg cagtgcgctc gtcgccgcac
    13981 accaaatcaa attcgcacga caaaggtttc agcacgatat tttccgcgcg gacatcgccg
    14041 ctatcgacgc cgaaagtgca cgtattcaaa ttaaacgttg ccgttttgaa gacagccata
    14101 ttggcatctt cttgaggaat cagtgccatg ccgtctgaac ataagcctgc ataaatctcg
    14161 cttttcgctt tggcaatatc gcccactccg tcgaaaccgc atccgacatg ggcgcgcagg
    14221 gcgttgttga ccaaagcggc atcgggtttg gcgatttgcg ttaaaaccgc cagttcgcca
    14281 aaatggttca tgcccatttc aatcacggca tagcggtgtt tttcgtttaa tttcaataaa
    14341 gtcagcggca atccgatgtg gttgttgaag ttgcctgccg tcgccgaaac ggcatcatcg
    14401 ccgaaacggc ggcgcaatac cgcagccagc atctccttca ccgtcgtctt gccgcccgaa
    14461 ccggtaatgc cgaacacaaa cgggttcaca ttatcgcgcc acgccttcgc caacgtttgc
    14521 aacgcggcaa gcgtgtcatc gactttcaac gcgccgccca aagccgcgca atcttcgcgc
    14581 gaaaccacaa ccgccgccgc gcccgcagac aatacgcctc caacaaaatc atgcgcgtca
    14641 aaccgcccgc ccgccaatgc gaaaaacaca tcgccttccc gaatatcgcg gctgtcggtt
    14701 acgatgcgcg acacgggttt gttttcagac ggcatcggaa gcttgagggc ttggcagatg
    14761 aaatttaggt ccagtggttt catatttgct ttcgttaata ttcgggcggc ggacacatcg
    14821 gtagcggctg atttttttat cgcctgtttt gctgtggtaa aacacagatt attttcccat
    14881 tctcattcgg cattttttct gtacgtatca ttttttagac gtatttttag ccgatttgcc
    14941 ttttcccgca taccacggcg cggggtcgtc ggactgtctg tcgataaagg caaggttatt
    15001 gccttcgccc ggcacatcgg ggacattccc ccaaaaatca tagccgtcat cgggcaactc
    15061 gtcggtttcg atacccgtcc aactgccgaa tccgcgtaaa aaattaaacg cgctgcgcct
    15121 cactttaatc atttcgcgcc gcgtgtcatc ggacgaaatt ttaaccagcg gcacgccgta
    15181 gctttgacgc ttccactcgc cgtgtgtcaa cgttcgctcg ccgcctttgc cgacatgcat
    15241 caaaccgtgg tcggaaaagt aaaccaaaga ccagctttct ttattttcat tcaatatctt
    15301 aaccgtatct tctaaaaatt tatcggtttg cgcgatggtg gaaacatagc aggatatttt
    15361 ttcagtttga tactgaaacc gccgcgcatc cttatccaaa cgtgtgcaaa aatcactgtg
    15421 cgaacccatc aggtgcatca caatcagccg aggcttcgtg cctgcatttc ctgtcaaaac
    15481 ccgtttgaac gccggcaaca aaaggcggtc gctcaacccc gcgcttttgc cataatcacc
    15541 cctttgggta aaccacggat aatcgctgcg tagggcatag gtggaaattt cgttggcaaa
    15601 atgccccaac attccttgat tagacagcca cgccgtccga aaacccgcct gcttcgccaa
    15661 gctgacaatg ttattgttcg gctctcccgg cagccccaaa gtctgcggca gcgaaagatt
    15721 cgtcgcgtgg gcggtcgatt ggtaaccgtt tatcaacagc cctttggtcc gactcaaaaa
    15781 aggcgtatcg ggcaatggga aaccgtaaac attcatataa tccgaacgcg cgctctcacc
    15841 gataaccaca acataatttt tatacttggg cgcaacatga cggatatgcc atgttgacgc
    15901 tttttttgct gcttccaaaa tgtgggcgcg cttggcggca tattgtgccg gcgcggaagc
    15961 caaatcgtaa tacaggtcga aaatattcaa caacaggccg gcatcaggtt ctcgccaatc
    16021 tttatcgccg gcgattttct ccatcaccgc gcaggataaa atcaaagtca ataatatggt
    16081 cagccatatt ttgctgcggc gtttatagtt ttttacgtca gcaaataccc ccacagatac
    16141 acaatatttc caaactgtca agccaaaaat gaaaaataat gcctgcacaa aataaagcga
    16201 cccgggaaga ttgccgacaa attcacgcgc ctcggcagga ttgctttcca atatcgaacc
    16261 gactatctga taagaaggcg caccatacag ccagccgacc ggcaaatata gggcggttgt
    16321 gccgacgtaa atcagtaaaa caactgaaga aacgcgcggg aagccgcgtg ccaataataa
    16381 taaaaataca accgaagcca aagcgcccac ggcagaataa cggtagccgt attcatattc
    16441 caagtgataa cctgatgcga tggccgcgcc aaaacaaaag gctaaaagcg cgcaaatgcc
    16501 caatgtattt tttaatgtct gatccatatt ttttattcgg gcggattgtt tcatacgatg
    16561 ccgtccgaat atcccattat ttacgagtta acaaagcctg cccgacgatt tcaagatcgg
    16621 aaaaacggtg cttcacgccc tgtacatcct gatagttttc gtgtcctttg ccggcaatca
    16681 ggatgatgtc gtttgcggcg gcctgctcaa ccgcataacg gatggcggcg gcacggtcgg
    16741 cttcgacgca ttcgggcgcg ggcacggcgg gcaggatgtc gttgatgatg tcttgcggat
    16801 tttccaaacg cgggttgtcg ctggttacga cgactttatc cgcgccctgt acggctgccg
    16861 cgcccatcaa tgggcgtttg ccgcaatcgc ggttgccgcc gcaaccgaat acgcaccata
    16921 aggccgcacc ctgcggtttg atttcctgca aagtggagag tgctttttcc aatgcgtcgg
    16981 gcgtgtgggc gtaatcgacg acgaccaagg gcttgccgct gttcatgatg cagtccatgc
    17041 gccctgaagc gggacggatt tttgccagca catccaatac cttatcaagc ggataaccgt
    17101 tggcacaaag caaggcgatg caggcggcga ggttttgcgc gttgaaccgt ccgagcaggc
    17161 gggtgcggca ttttccttcg ccccacgggg ttcggaatac ggcttctata ccgtctgaag
    17221 aggcggtgaa atcggtaatg cggatgtcgg cgtgttcgct gaaaccatag ctgtaaacgg
    17281 ctaaatcggg acagtctttt ttcagacgac ctgcgagttc cgcgccgtat ccgtcatcgg
    17341 tattgataac ggcgtgtttc aagccgtgcc agtaaaacag gcgcgacttg atagcaccgt
    17401 aggcttccat cgtgccgtgg tagtcgaggt ggtctcgggt gaggttggtg aagattgcgc
    17461 tgcggaacgg cacgccgttg actcgcgact ggtcaaggcc gtggctggaa acttccatcg
    17521 cggcggctgt tgcgccttgt tgacggaaac ggtagagcag ggtttggaca tcgacggggg
    17581 cgggggtggt gtgcgcggtt tcttccaatg caccccaaaa gccgttgccg accgtgccga
    17641 taatggcggt tttttcgccc aacaaatcgg cggcttgcgc cagccattgc gtgatggagg
    17701 ttttgccgtt ggttccggtt acgccccaga ctttgaggcc gcctgaaacg ttgccgtaaa
    17761 cttgcgccgc caacataccg gcacggtgtt tcaaatcttt gatgccttga ttggggactt
    17821 tccattcggg attccacgca aatttgccgt cgtcgtccca aaaaacaaaa gccgcgccgt
    17881 tggcaatggc tgcggggata taactgcggc cgtcggtata ttcgccctga caggcaacga
    17941 aaatatcgcc ttgtttgatt tggcggctgt ctgaatgcaa caaacgccct gccgcgtttg
    18001 cacacggcag gggcagaatg ccggtttcag ccaaaggggt taacttgctg aacataaaac
    18061 aatctcgttg atacttggat taagacggtg ttttgacggc tgcggcggtc aggggcttgg
    18121 tcggagaaac gcccaagatg ttcaggctgc cacccataat ttttttgaag ggcggccctg
    18181 tcactacgcc gccgtagtaa ccgtttgcag tcggttcgtc gatggttacc gccacaatca
    18241 cacggggatt tttggccggg gcaaaaccga tgaaagtggc aacgtgtttg ttatcgacgt
    18301 atcgtccgtt aaccaactta cgcgccgtac cggtttttgc gccgacatcg aaaccatcca
    18361 cagcaccggc agtaccggta ccgccggctt cagtaacgga aaccatcaac tcgcgcactt
    18421 ttttcgcagt agaggctttg atgacgcgct tgcctttagg ggcaactgct tgtttttcaa
    18481 agctgaccgg caacaattcg ccgtcatggg tcaagacagt ataggcacgc gccaattgca
    18541 acaggctcaa ttgcaggccg taaccgaaag acatcgtcgc ctgttcgata ggccgccacc
    18601 tgcgccaatt tctcaacaaa cctgcagttt cccccggaaa gcccgagtgc atacgcacac
    18661 cgatgcccaa ttcatgatag aagtcataca tttcttcggc gccgaaacgc gcagacagtt
    18721 tgcttgtgcc gacgttggac gatttctgca taatgccgcg cacatccaaa gaggggtaaa
    18781 catgggtatc gtcgcgcacg ggagacggtc cgattttata aggctgcgta ttcagccgtt
    18841 cgttcaaatc ggttttgccc gcatccaatg ccttcgcaat cacgaacggt ttgattgccg
    18901 aaccaggttc gatcatatcg gttacggcac ggttgcgccg ctgttcgctg tctgcccggc
    18961 cgggtctgtt gggatcgtag gcgggcgtat tggccaaggc gaggatttcc cccgtgcggg
    19021 catccaaaac caccaccgtt ccggcttttg cctgatggta ttcgaccgcc ttgttcaact
    19081 cttcataggc caaggtctga atcctctgat cgagggaaag gatgatgtct ttgccgtttt
    19141 gcggtgcttt attgcgcggg gagtccaagc tgtccacaat attgccctgc cggtcccgca
    19201 aaacaacttc cgcgccgtct tcgccataca ggctgtcttc aagcgaaagt tccaaacctt
    19261 cctgaccttt gccgtcaata tcggtaaatc cgatgacgtg tgcaaacagg ttgcccatcg
    19321 ggtaatggcg ttttaattct ttttcaaata caaagttttc caaacccaag gctttgacct
    19381 cttcggcaac cttgggatcg agctgccgct tgatccaaat aaacgacttg cctttctgtt
    19441 cgagtttgtt cctcaaaaca tcgaccggca catcgacaag ctcggacagg cgttccaatt
    19501 gggcggcaga cggcatttcc ttcatatctt taggcacggc aaacagggac tccgtcggcg
    19561 cgctcaacgc caaaaccgca ccgttccggt ccgaaaccgt accgcgtgta gccggcaatg
    19621 cttgagtccg cacaatccgg ttgtcgccct gttctttcaa aaagttatac gttaccgtct
    19681 gcagatacag cccgcgggca atcagacagg caaacaagac cgccattgcc atcaggacga
    19741 agctaatccg tccgttactg gtcatcggct ttttgacctg ctcttctttg ggcagcatcc
    19801 ggggcttata ttcgctttta atcaacattt ttacttctcg ttattattat cctgatgcag
    19861 gaatccgatt ccggcacaca ggctgcttct atctttgatg ttccaccata aaggtattgc
    19921 ccgaaaccgg cggatggagg ttttgttttt ctgccgccgc cctgatcgct tcgtgattcg
    19981 ccaaacgcgc ctgttgcacc ctcatttgcg cataatcctg ctccaaggcg atttcctgtt
    20041 ttttcgcctt gtccaaagct gtgaagttga gcctgtactg gttttgctgc atcacaacgg
    20101 aaaaagcgga aacgcacacc gcaagcagca gaaagaaatt cgatttgttc attgccgttc
    20161 agacgttttt ctctgttatt attccggtat cggaccggca gtccgctccg ccacacgcaa
    20221 aactgcgctc ctcgccctcg ggttggcggc aatttccgct tcacccggct ttaatgccct
    20281 gcccacgatt ttcaggggcg gctcgggcaa atccgcttct ctgaccgccg cccagctcgg
    20341 caggggctcg tgttgcgaat attttttgac aaactgcttc acaatgcggt cttccaacga
    20401 atggaaagca atgaccgcca aacgcccgcc ctctttcaga cggcacatga cctgcggcaa
    20461 taccgcccct acttcttcaa gctcgcggtt aataaagatg cggattgcct ggaaggtgcg
    20521 cgtcgcagga tcctgccccc gctcgcgagt acggacgttt tgtgccacga tctgcgccag
    20581 cttgcgggtt gtatcgattg gactttccgc ccgttgcgcg acaatggcgc gcacaatctg
    20641 gcggctaaac cgctcttcac cataattctt gattacctcg tgcaaatcct gttccgacgc
    20701 aaccgctatc cactctgcgg cagacatacc gcgcgtcgta tccatacgca tatccaaagg
    20761 ggcatcgaaa cggaagctga aaccgcggct gccgtcatcg atttgcggag acgaaatccc
    20821 caaatcaaac agcgcaccgt ccaccttgcc gatacccaaa ccgtccaatg ccgtctgaaa
    20881 cgaagcaaaa ccgccatgca cgacaccgac ccgtttgtcc gaacgcgcca gctcttctgc
    20941 cacagcaatc gcctgcgggt ctttgtcgaa aacaatcaac cgccccgcat cgcccaaacg
    21001 cgacaaaatc agccgggaat gccctcccct gccgaacgta ccgtccacat agacaccgtc
    21061 ttcgcgcacg gcaagcgcat ccaccgcctc attcagcaag accgtgatat gccggtaact
    21121 ttctgctcca ctcacaattg caaatccgtc tgactcaact ggaaggcaag ttcgtcagga
    21181 tcgtcatcca aagcctgaac catctcagcc tcccactgct cgcgacccca aagctccaaa
    21241 cggttggcac ggccgaccaa aacaacttca cggtcgaaat ccaccctttt cctcagtccg
    21301 gcaggaacca gcaccctgcc ggcgctgtcc cattccaaaa tttccgcgtt atgcagcaaa
    21361 agattttgaa accgccgcaa aacagggtta tccgccactt ttaagtttaa aagttgcgcc
    21421 gcaacctttt cccactccgc aacagggtac atcaacagct tatgtttcga ctcgagcgtt
    21481 gccactacgg caggcgtata gaggcgcgac agaatgtcgc ggaatttggc aggaacagcc
    21541 aaccgcccct tactgtcgat gcttaattcg tgtgcgccgc cgaacatgac atgccccaag
    21601 ccgaaatcaa aatcacaagg gtaaaagaga cactttgccc cacaattccc caccaatcga
    21661 cactataaga aattttaaac actcggtcaa atcagggcat gaaaactcat taacatatct
    21721 gaaattttta ttctgtttaa aaacaataag ataaaaaatg acgacaacgg cacggcgggt
    21781 gcagtacaga gtgaccgaac caataaacaa ctatatatga ttaatttaat aatataaaca
    21841 caatatatag tattaagata aagccatgac agcacccgta ccaacgtgta atatgtcggg
    21901 aaatccaata aatttacaca agctaacatt tatcatgctc ctcccctccc ccgaagcacg
    21961 gcaatcctcg ctcaatctgc aaaccctcat tgccgaagaa atcggcaaac acggcaactg
    22021 gattccattt tcacgtttta tggaattggt tttatacgct ccgcaatacg gctactacac
    22081 cggcggcagc cataaaatcg gcaataccgg ggattttatt accgcaccga ccctcacccc
    22141 tctgtttgca cagacactgg cacgccaact tcaagaactc ctgccccaaa cggcgggcaa
    22201 tatctatgaa ttcggcgcgg gaaccggaca gcttgccgcc gatttgttgg gcagcgtttc
    22261 agacagcatc aactgttact atattattga aatatcgccg gagctggcag cacgtcagaa
    22321 aaacctgatt caagcccgtg caccggaagc atctcaaaaa gttgtccact tgaccgcact
    22381 tcccgaagcg tttgacggca tcatcatcgg caacgaatta ctcgatgcca tacctgtcga
    22441 aatcgtccgt aaaaatgaag gaggattact cgagcacatc ggcgtttgca cggataacgg
    22501 ccgtttcgcc tattctgcaa gacccttgca cgacccgtcg ctttccacct cggcttccct
    22561 ctattttcct caaacagatt atccctatac cagcgaactg cacccgcaac aatatgcctt
    22621 tatccgcacc cttgcctcaa ggcttgaacg cgggggtatg atattcatcg actacggttt
    22681 tgatgcggcg cagtattacc acccgcaacg caatcaaggc acattgatcg gacactaccg
    22741 acaccacgtt atccacaatc ctttcgactt catcgggttg gcagatctga ccgcccacgt
    22801 caattttacc gacattgccc aagccggtac ggatgcagga ctggatttga caggctatct
    22861 gccccagtcc cattttctgc tgaatctggg cataaccgaa ctgttggcac agacgggtaa
    22921 aacagattcg gcagcctata tccgtgaagc cgccgctgtt caaaaactga ttgaccagca
    22981 tgaaatgggc gaactgttta aagtcatcgc attcggaaaa aatatcggca tcgactgggc
    23041 aggattccgc ttcggcgaca tctgccacaa actccaaccc tcgtgccgtc tgaatccgct
    23101 tcagacggca taaacttttt aacatttaaa aacaagaaac taattcaaaa ttaaaaaata
    23161 cggcttgtca aaaaaacaaa aaaacatata atagctaatt cacgaaacgg cgaattagct
    23221 cagtcggtta gagcagagga atcataatcc ttgtgtccgg ggttcgagtc cctgattcgc
    23281 taccagattt tcgggggtat agctcagttg gtagagcgct tgcatggcat gcaagaggtc
    23341 agcggttcga tcccgcttac ctccaccaga taaaaaagca cagaccgtaa aaggtatgtg
    23401 cttttttatt gcctgattgc cggaaaataa agaataaacc actgccttca aaacaggcaa
    23461 tcgactttaa acctattgcc ccgcctgtcc tgattttatc agcccgccag acaaacccga
    23521 cctgaataat gtcttcaggt cgggtttatg gtttcattcc caacttatcc agcccgacaa
    23581 ccacaatatt atgatgacaa tgccgaaaac aatgcggtaa taggcaaacg ggatatagtt
    23641 tttcttggaa acaaacttca gcagtgcttt aaccgctacc aaaccggaaa caaaagcggc
    23701 aataaagcct atcaaaatca aaccgacatc atgcagggtg aaaaatcggt aatgtttcag
    23761 gacatcataa gccgttgctg caaccatcat cggaacggcc aagaaaaatg aaaactccgt
    23821 tgccgttttc cgctcgattc cccaaagcat cccgcccata accgtactgc ccgaacggga
    23881 cgtacccgga accagtgcaa acacttgggc aacaccgatc atcaacgcat caatcggacg
    23941 caatgcatca acatcggcaa ttttaggctc tgctcggctt tggcgtttct ccacccacaa
    24001 aataaaaaaa ccgcccaaaa ccagcatgac tgcaacactc aaggggttaa acagatactc
    24061 tttgatttgt ttgtcgaaca acagccccat cacggcggca ggtataaaag caatggcaag
    24121 attgaggacg aaacggttgg ctttccggtc ttttcccacg ccatgcaaca cattgctgaa
    24181 acgctgccgg tattcaaaca ctaccgccaa aaccgcaccg agctggatgg caatttcaaa
    24241 aaccttgtga ttgctgtgaa aaccaatcag attgccgaac acaatcaaat gtccggtgct
    24301 ggaaatcggt aaaaattcgg taaaaccttc taccaagccc atcatcaggg ctttcaggac
    24361 aatcagaaaa tccattgctt gcgcttcttt cggatacggg agttcggcta tttctgtaca
    24421 gcaggggtct gacgcttgcg ttcttccctg actttggcaa ccaattcttt aatcgtatga
    24481 acgccgccat caaagccatt attgaagata acgcggtatt ttccgccgac aataacggtc
    24541 ggcgtgctgt caataccgta ttgttccgtc agtttctgca tttttaatgc gacggcggca
    24601 gcttcggggg aatcataggc gcgcatcagt tttttgccgt caaaaccttt ttgagataaa
    24661 gcccattttc cggcaacagc cctgttttcc aaacggattt tttgttcgta aactgcttta
    24721 aacacagcag agtttgcctg atatttcaaa cccgacagct tgaccgcagc agccattctt
    24781 gccagaccga gcatttcagg ccgccagacc acgtgctccg tccgcagata agtatcagac
    24841 ggcaatgcct tgcccagttt caataacaaa ggatcgaaat gatggcaatg tacgcaaaaa
    24901 tagccgaaaa attccaaaac ctcaattttt cccggctgtt cttgaggaat gggtttatcc
    24961 aacacaagat agtcttcccc ttccgtcagg gcatatgcct gcgcggacaa cactgccgac
    25021 agcagcagcg gcaacagatg tttaaacttc ataattattt gccttcaatc gcacggatca
    25081 ggctggcaac cccatgcttt ttcaactcgt cctgcatttt tttcaccgca tcggcagaca
    25141 tattgccgct ttgcacgcgg taaagcgttt tatgtcccgc ctgatagccg accacttcgg
    25201 aagatatgcc caagattgcc agtttggcac gctgcccttc cgcgctccgg cggtcggcat
    25261 acgcgcccat ttgcagataa tgcgttgctt ccgccttgcc aaacgttttc attttctgca
    25321 cttctttggc agcggcactg cgcgcttttt cgatgctgcc gctgttgagg atttgttccg
    25381 gggtcggttt gggtgcaact ttttccttcg ccgccttttt ctcttctttt gaagcttttt
    25441 tctctgtttc tttagacggt tttaccgctt gttttttaac cgtttcggca tctttcttct
    25501 gcgctttttc cctgacggtt tgttcacgct cttccgtcag tgctttcttg cgcactgcct
    25561 gtccgtccgg ctcttcccgt tccggctcgc ccgccttttc ttcaacctcg tcggctttgt
    25621 cggcaacggg ctgcttgtcg gcagcttttt ccgcatccga ctgctctgcc tctttcgcaa
    25681 catccggttc ggacaaggcg ttttgatcgg ccggttcagg ttggatgtct tccttaggct
    25741 ggtttttcag tttcaggatt tccgtttctg caggctgctt cgacggagcc gggattttga
    25801 acgcattttg accgccctgg ttcagataaa gcaaaatacc ggcaataatg accgttgcca
    25861 gtatcaaacc gaagaagaaa ccggacagac cttttccgga ttgggaaaat ttgttcataa
    25921 acatacctta atgtgtttca gacggcatta acgccgtttg cttacgggcg gatattctaa
    25981 caatatcgcc atatttgggc aaaacctgct tccattcccg ttcctataaa gcacgacgga
    26041 agcctatggc tttcatgacg tggcttctgc cgacttcttc atcgcccgcc aaatccgcca
    26101 atgtacgcgc cacgcgcata atgcggtgga agctgcgggc ggaaagagac aatttttcca
    26161 gcaggctacc caaggcttcc tgcgcttctt tttggatacg ggcttgggaa tcgagttcgc
    26221 tgacgctcaa agcggcgttg actttgccct gccgtgcgta ttgtttgccg cgggcggcga
    26281 tgacgcgttc caaaacggac gcactgcttt cgcccgcttc ctgctgcatc agttcggcgg
    26341 cggacaggct ggggacttcg atggtcaaat cgatgcggtc gagcagcgga ccggaaattt
    26401 tgcttcggta acgcgcgaca ctttcgggcg tgcagcggca gggtttgacg ggatgcccga
    26461 gataaccgca cggacacggg ttcatggcag cgacaagctg gaatttggcg ggatagacgg
    26521 cttggcgcgc cgcgcgggaa atgtggattt cgccgttttc caacggttcg cgcaaaacct
    26581 ccaaaacttt gcggtcgaac tcgggcaatt cgtccaaaaa taaaacgccg tgatgcgcca
    26641 atgaaatctc gcccggacgc ggatccgaac cgccgccgac catagccgcc gcgctggcgc
    26701 tgtgatgcgg actgcggaaa ggacggttgc tgtcgagttg ttgttggtgg ttgggcagga
    26761 gcgaacgcaa tgcccaaact tctaccaatt cgtcttcggc cagcggcggc aggatgccgg
    26821 gcagccgttg ggagagcata gacttgcctg ttcccggcgg gcccatcatc aagaggctgt
    26881 gtccgcctgc ggcggcgatt tccaaagcaa agcgggcagt gtgctgacct ttcacatcac
    26941 acaaatcagg ttgtccgcca tgttcagacg gcatttgcgg tacggaacat tcggtttgcg
    27001 ccaaaggttc gataccgttc aaatgagcgg cgacttcgcc caaagagcgc gcgccgtaaa
    27061 cggtaatgcc gcgcatcacg gcggcttgtc ctgcgttttc ttcaggcaaa acaaatgcac
    27121 gttttgcctg cataccctgc cacgccatcg ccaacgcgcc gcgcacgggg cgcaacaacc
    27181 ccgacagtgc caattccccc gcaaactcgt attccgccag cttttcgggt gcaacctgcc
    27241 ccgatgcggc aaggatgccg attgctatcg gcaaatcgaa acgccccgac tctttgggca
    27301 ggtcggcagg ggcgaggttg acggtgattt ttttggcggg gaattcaaaa ccgctttgaa
    27361 taatggcggc acggacgcgg tcgcggcttt cctttacttc cgtatcgggc agtccgacga
    27421 tgttgaaatg cggcaggccg ttggcaaggt gggcttccac ttcgaccaac ggcgcattca
    27481 taccgctcaa ggcgcggctg taaaccaagg caagcgacat atttcagacg gccttattcg
    27541 ccggcttcgg tttgctgcct gatttcggcg acggcttctt cggcagcggc ttcagccgct
    27601 tccaatgctg cccgttcggg attttgcgcg gcttcgagtt tcgccagacg tgcttccaaa
    27661 gccgccagtt tggtacgggt tttaatcaaa acctgctgct ggatgtcgaa ttcttcgcgc
    27721 gtaaccaaat ccatacggtt gaacgcgccg cccagcatcg ccttaatatt tttttccaca
    27781 tctttggcag ggctgttggc gatggtttcg ctgattttcg agccgacttc ttcaaaaagc
    27841 tgcttgccga acataatctg catccttcct gaacatatca aattcaagcg gctattgtat
    27901 aaggaaaaat gccgtctgaa aacgggcggc ggataatcgt caaaacatac cgcgccccct
    27961 ttgcggttgc taaaattaca cttcgagaca cctgccgcct gaaaacggat ttcatattcc
    28021 gcgcaacgcc cgattacatt acaagacact acaaaacaat atgctgtttt aaatgatttt
    28081 tccgatgcgc atcgtttcaa actcggcttt ttaagccatt aagtgctttg caaacaacag
    28141 aaattgggtt atgctgaaac ggattattta caatttcata tagtttcatt acatatctta
    28201 ttgtaattga agataattta tccgaatccc ctttcgggta tccggatttt ccgttgtact
    28261 ttcattagaa aaacatttca ggcgcaagtt gcttgcaatt tcaaagcctg taattctgaa
    28321 gttttagacg aggaatagca catgaatccc atgtacatca cttttgcaat ctatttggtt
    28381 gcagtccttc tcatcgggct tgccgcttat ttttccacgc gcaatttcga tgattatatt
    28441 ttgggcgggc gcagcctggg cccgtttgtt acggcgatgt cggcaggcgc gtccgatatg
    28501 tccggctggc ttttgatggg tctgccgggc gcgatttatt tgagcggttt gaatgaggct
    28561 tggattgcca tcggcctctt ggtcggcgcg tatttcaact ggctcttggt ggcgggccgc
    28621 ctgcgcgtgc ataccgaata tgccaacaat gcgctgacgc tgccggatta tttcttccac
    28681 cgcttcggcg cgggcggaca cttgatgaaa gtggtttccg cactgattat cctgtttttc
    28741 ttcacgattt attgcgcctc gggcattgtg gcgggcgcaa ccctgttcca aagcctgttt
    28801 gaaggtatga cttacaatca ggcaatgtgg ctgggcgcgg gcgcgaccat cgcctatacc
    28861 ttcttgggcg gcttcttggc ggtcagctgg acggatacgc tgcaggcttc tttgatgatt
    28921 ttcgccctga ttttaacgcc ggtgatggtc tatctgggct tgggcggcgc ggaacagatg
    28981 tctgccgcaa ttcaaagcgt cgccgcaggc acgggcaaag aatacggcag cctgtttgcc
    29041 ggtacgaccg tcatcggcat catttccacc gccgcctggg gcttgggcta tttcggacag
    29101 ccgcacattt tggcgcgctt tatggccgcc gaaagcgcga aatcgctggt atccgcacgc
    29161 cgcatcggca tgacttggat ggcgttgtgc cttgcgggcg cggtagcggt cggttatttc
    29221 ggcattgcgt atttcggtgc aaaccccgac aaagtttctt ctatgagcgg caaccacgaa
    29281 cgcatcttca tcgcgctttc caccctgctg ttcaaccctt ggatcgccgg catcattttg
    29341 agcgcgattt tggcggcggt gatgtccacc ctgtcctgcc agcttttggt ttgctccagc
    29401 gcgattaccg aagacttcta caaaggtttt ttgcgtaaaa acgcgcaaca gtcggaattg
    29461 gtatgggtcg gccgcctgat ggtattggcg attgccgtga tttccatcct gattgcttcc
    29521 gatccgaaca gcaaagtatt gggactggtg tcttacgcgt gggcaggttt cggtgcggca
    29581 ttcggcccga ttgtgattct gtccgtattg tggaaacgca tcactgccta cggcgcgttg
    29641 tcaggcatgg ttgcaggtgc gtcaaccgtt gttgtgtggg cggaatgggt aaaaaaaccg
    29701 gctcgagcgg caggcgaaag cggcttgttg acgatgtacg aaatcgtccc gggctttatt
    29761 gtttgcttga tcgttgccgt gttggtttcc ctgttcaaca aagaaccttc gcgcgaaatc
    29821 caagaacgct ttgaaaaagc cgacgcagat taccgcgccg cccgataaac ccctttcaga
    29881 cggcacggca tcccggttcg tgccgtctga acccgcttcc gggccaaaca caaataaacc
    29941 ttgcctgcat acaggcctta tttgtgtttt acccgtttca ttttcaaaga aacggaagca
    30001 ccgattctgc ccgcatttga agccttgtat ttcatctgcg gatttattac gatattaccg
    30061 tattcaggcc gcaccgatgc cgtctgaacc ccccgaaaaa actttggagg atccaaaaaa
    30121 tgtttcattt tgcatttccg gcacaaactg ccctgcgcca agcgataacc gatgcctacc
    30181 gccgtaatga aatcgaagcc gtacaggata tgttgcaacg tgcacagatg agtgacgaag
    30241 agcgcaacgc cgcctccgag cttgcccgcc gtttggttac ccaagtccgc gccggccgca
    30301 ccaaagccgg cggcgtggat gcgctgatgc acgagttttc actctccagc gaagaaggca
    30361 tcgcgctgat gtgtctggca gaagccctgc tgcgtatccc cgacaacgcc acgcgcgacc
    30421 gcctgattgc cgacaagatt tcagacggca actggaaaag ccatttgaac aacagccctt
    30481 ccctcttcgt caatgctgcc gcctggggtc tgctgattac cggcaagctg accgccacaa
    30541 acgacaaaca aatgagttcc gcactcggcc gcctgatcgg caaaggcggc gcaccgctca
    30601 tccgccaagg cgtaaattac gccatgcggc ttctgggcaa acagttcgtc accggacaga
    30661 ccattgaaga agccctgcaa aacggcaaag aacgcgaaaa aatgggctac cgcttctcct
    30721 tcgatatgtt gggcgaagcc gcctacaccc aagccgatgc cgaccgctac taccgcgact
    30781 atgtcgaagc catccacgcc atcggcaaag atgcggcagg acaaggcgtt tacgaaggca
    30841 acggcatttc cgtcaaactc tccgccatcc atccgcgcta ctcgcgcgcc caacacggcc
    30901 gcgtgatggg cgaactgttg cctcgcctga aagaattgtt ccttttgggt aaaaaatacg
    30961 atatcggtat caacatcgat gccgaagaag ccaaccgtct ggagctgtct ttggatttga
    31021 tggaggcact cgtttccgac cccgatttgg ccggctacaa aggcatcggt ttcgttgttc
    31081 aagcctacca aaaacgctgc ccgttcgtca tcgactatct gattgacctg gcccgccgca
    31141 acaaccaaaa actgatgatc cgcctcgtca aaggcgcgta ttgggacagc gaaatcaaat
    31201 gggcgcaagt ggacggcttg aacggctatc cgacctacac ccgcaaagtg cataccgaca
    31261 tctcctacct cgcctgcgcg cgcaaactgc tttccgcgca agacgcggta ttcccgcaat
    31321 tcgccaccca caacgcctac accttgggcg caatctacca aatgggcaaa ggcaaagatt
    31381 ttgaacacca atgcctgcac ggtatgggcg aaactttgta cgaccaagtc gtcggtccgc
    31441 aaaacttagg ccgccgcgtg cgcgtgtacg ccccggtcgg cacacacgaa accctgctcg
    31501 cctacttggt gcgccgcctg ttggaaaacg gcgcgaactc gtctttcgtc aaccaaatcg
    31561 ttgatgaaaa catcagcatc gaccgcctga ttaagagccc gttcgacacc atcgccgaac
    31621 aaggcatcca cctgcacaac gccctgccgc tgccgcgcga tttgtacggc aaatgccgtc
    31681 tgaactcgca aggcgtggac ttgagcaacg aaaacgtatt gcagcagctt caagaacaga
    31741 tgaacaaagc cgccgcacaa gacttccacg ccgcatccat cgtcaacggc aaagcccgcg
    31801 atgtcggcga agcgcaaccg attaaaaacc ctgccgacca cggcgatgtt gtcggtacgg
    31861 tcagctttgc cgatgccgcg cttgcccaag aagcggttgg tgcagccgtt gccgcgttcc
    31921 ccgaatggag tgcgacacct gccgccgaac gcgccgcctg cctgcgccgt ttcgccgacc
    31981 tgctcgaaca gcacaccccc gcgctgatga tgcttgccgt gcgcgaagcg ggcaaaacgc
    32041 tgaacaacgc cattgccgaa gtgcgcgaag ccgtcgattt ctgccgctac tacgcaaacg
    32101 aagccgaaca caccctgcct caagacgcaa aagccgtcgg cgcgattgtc gccatcagcc
    32161 cgtggaactt cccgctcgcc atctttaccg gcgaagtcgt gtccgccctg gcggcaggca
    32221 acaccgtcat cgccaaacct gccgaacaaa ccagcctgat tgccggttat gccgtttccc
    32281 tcatgcacga agccggcatc ccgacttccg ccctgcaact catcctcggc gcaggcgaca
    32341 cgggtgcggc attgaccaac gatgccagca tcggcggcgt gattttcaca ggctcgaccg
    32401 aagtggcgcg cctgatcaac aaagcccttg ccaaacgcgg cgacaatccc gtcctgattg
    32461 ccgaaaccgg cggacaaaac gccatgattg tcgattccac cgcacttgcc gaacaagtct
    32521 gcgccgacgt attgaactcc gccttcgaca gcgcgggaca acgctgctcc gccctgcgta
    32581 tcttatgcgt ccaagaagac gttgccgacc gtatgctcga catgatcaaa ggcgcgatgg
    32641 acgaactcgt cgtcggcaaa ccgattcagc tcactaccga tgtcggtccc gtcatcgatg
    32701 ccgaagcgca gcaaaacctg ttgaaccaca tcaacaaaat gaaaggcgtt gccaagtctt
    32761 accacgaagt caaagccgcc gccgatgtcg attccgaaaa atccacgttc gttcgcccca
    32821 tcctgtttga attgaacaac ctcaacgaac tgcaacgcga agtcttcggc cccgtcctgc
    32881 acgtcgtccg ctaccgcgcc gacgaactcg acagcgtcat cgaccaaatc aacagcaaag
    32941 gctacgccct gacccacggc gtacacagcc gcatcgaagg cacggtacgc cacatccgca
    33001 gccgcatcga agccggcaac gtttacgtca accgcaacat cgtcggtgca gtcgtcggcg
    33061 tacagccctt cggcggacac ggtctgtccg gcacaggacc caaagcagga ggttcgttct
    33121 acctgcaaaa actgacccgc atccccgaat gggttgcccc gaccctgagc caaatcggac
    33181 aggcagacga agccgcgctc aaacgcctcg aagccctgat tcacaaactg ccgttcaacg
    33241 ccgaagagaa aaaagccgca gcggccgctt tgggacacgc ccgcatccgc accctgcgcc
    33301 gggcagaatc cgtcctgacc ggcccgaccg gcgagcgcaa cagcatctca tggcacgcgc
    33361 caaaacgcgt ctgggtacac ggcggcagcc tcctgcaggc cttctgcgcc ctgaccgaac
    33421 ttgccgccgc cggcatccga accgtcgtcg aacccggcag cccgttggct tcctactcgg
    33481 ccgacttgga cggtttgctg caaatcagcg gcaaacccga aaacaccggt gtgaaccacg
    33541 tcgccgccct aagcccactg gacagcagcc gcaagcagga actggcaaac cgcgacggcg
    33601 cactcatccg catcctcccg tccggacaag gcttggacat cctgcaagtg tttgaagaaa
    33661 tttcttgcag catcaacacc acagccgccg gcggcaacgc cggcctgatg gcggttgccg
    33721 actgattttg ccaaaccgcc cgggcgcggc ccgtgaacaa atgccgtctg aaaacctttc
    33781 aggcggcatt ttatagtgga ttaacaaaaa tcaggacaag gcggcgagcc gcagacagta
    33841 caaatagtac agcaaggcgg gccaacgctg tactggttta aattcaattc actatatgtg
    33901 ttggcacatc gctccaaacc
//
LOCUS       DBJGGI010000015        32320 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.015, whole genome shotgun sequence.
ACCESSION   DBJGGI010000015 DBJGGI010000000
VERSION     DBJGGI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32320
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.015"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(200..1039)
                     /locus_tag="ACSIGJ_000720"
     CDS             complement(200..1039)
                     /locus_tag="ACSIGJ_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HHQ1329660.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1324..1399
                     /locus_tag="ACSIGJ_000721"
     tRNA            1324..1399
                     /locus_tag="ACSIGJ_000721"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1357..1359,aa:Arg,seq:ccg)
     gene            complement(1464..2657)
                     /locus_tag="ACSIGJ_000722"
     CDS             complement(1464..2657)
                     /locus_tag="ACSIGJ_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HHQ1329661.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGRMP
                     VLESVSRAETARTAVPAPSPYLPMEGLDVYRSAVQHLLFGKGNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSERQWDEVLQIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMDLPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSSELYALWQNEVYMMRDRIRAMRQKLYGVLTARIP
                     DRDFTYFIKQRGMFGYTGLSVGQVRRLRDEFAVYLLDSGRMCVAGLNTSNITYVADAL
                     AEVLK"
     gene            complement(2677..3765)
                     /gene="trmA"
                     /locus_tag="ACSIGJ_000723"
     CDS             complement(2677..3765)
                     /gene="trmA"
                     /locus_tag="ACSIGJ_000723"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HHQ1329662.1"
                     /translation="MNDYTQQLQGKKDYLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASLIRCDRFDAASEAVNCLMPELIAVAAQSAELRNHW
                     YAVEFLSTLSGEMLVTMIYHKRLDDEWMKAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLRVGDRDFHYRQIEGSFTQPNAAVCRKMLEWACRAAEGLGGDLLELYCGNGNFTL
                     PLSRYFRQVLATEISKTSVSAAQWNIEANWIGNIKIARLSAEEFTEAYTGKREFTRLK
                     ESGIVLTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLTETH
                     TVGRAALFDQFPFTHHIESGVLLKKKIL"
     gene            3912..5012
                     /gene="aroC"
                     /locus_tag="ACSIGJ_000724"
     CDS             3912..5012
                     /gene="aroC"
                     /locus_tag="ACSIGJ_000724"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HHQ1329663.1"
                     /translation="MAGNTFGQIFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATAF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKKIRFEGSEHISQNPFFAANQSQIAELEHYMDGVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDIHGNPVELATRGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAADTPDISRSDK"
     gene            5103..5528
                     /locus_tag="ACSIGJ_000725"
     CDS             5103..5528
                     /locus_tag="ACSIGJ_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HHQ1329664.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(5602..7587)
                     /gene="parE"
                     /locus_tag="ACSIGJ_000726"
     CDS             complement(5602..7587)
                     /gene="parE"
                     /locus_tag="ACSIGJ_000726"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HHQ1329665.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATEIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVSVKRDGKVHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDGHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTSLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARIRSVKKIEKKKGSGVAILPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHAINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAQLDI"
     gene            complement(7654..8178)
                     /locus_tag="ACSIGJ_000727"
     CDS             complement(7654..8178)
                     /locus_tag="ACSIGJ_000727"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HHQ1329666.1"
                     /translation="MLDREGYRPNVGIILINERNEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQPSGNR"
     gene            8344..9639
                     /gene="serS"
                     /locus_tag="ACSIGJ_000728"
     CDS             8344..9639
                     /gene="serS"
                     /locus_tag="ACSIGJ_000728"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HHQ1329667.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPPGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYGALEEMVGHAENILKALELPYRVITLCTGDMG
                     FSAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVTKLEVK"
     gene            complement(9712..10710)
                     /locus_tag="ACSIGJ_000729"
     CDS             complement(9712..10710)
                     /locus_tag="ACSIGJ_000729"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HHQ1329668.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGLELEFFDFMLDAKTAKMAEG
                     AEAACIFVNDDASRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEVEKLGGRYVDLDELYARSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKCRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10835..11911)
                     /gene="prfA"
                     /locus_tag="ACSIGJ_000730"
     CDS             complement(10835..11911)
                     /gene="prfA"
                     /locus_tag="ACSIGJ_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HHQ1329669.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIDELDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGGEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRTDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREVQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(12001..12771)
                     /locus_tag="ACSIGJ_000731"
     CDS             complement(12001..12771)
                     /locus_tag="ACSIGJ_000731"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HHQ1329670.1"
                     /translation="MRLTDTHCHLADSVLRGNLAQVLAEARQAGVWRFIVPATCPQDW
                     QDVAELSEMPSEHGQIRIALGIHPWFSDGIAEQDFSGLEAVLVRYPRAWVGEIGLDFY
                     DKTQTPPQRERQIQVFSRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFTQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLENTV
                     NTPANILRIAEIAAEIRGTGAAEIAAITERNADSLLRP"
     gene            complement(12774..13766)
                     /locus_tag="ACSIGJ_000732"
     CDS             complement(12774..13766)
                     /locus_tag="ACSIGJ_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HHQ1329671.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSAGLRPDTALVGQALAPFSDGMDFD
                     WHVCEPLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANMFALALQGL
                     DKPIVLTGSQWPYNAEGSDAPRNLVTAVAAFPLGLKQTLIAFDGKLYPAVGSSKISTE
                     TAAGFGNAHFGALAEWDETRGWHNVRIPNQDAADVSDGLEIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTSAGEGFIRAVQDFTQQGKLLLNISQVPQGC
                     AAAVYAQGDALRRAGVVNGGKCNLETATALMTLAVSEGWGADGVKHELQRLGLV"
     gene            complement(13833..14516)
                     /locus_tag="ACSIGJ_000733"
     CDS             complement(13833..14516)
                     /locus_tag="ACSIGJ_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HHQ1329672.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGVMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14705..16039)
                     /gene="glmM"
                     /locus_tag="ACSIGJ_000734"
     CDS             complement(14705..16039)
                     /gene="glmM"
                     /locus_tag="ACSIGJ_000734"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HHQ1329673.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNTYSDNGIKFFAEGGVKLSDEVELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPGHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGNEPNGYNINEK
                     CGATHTKTLQAAVLQNEADYGIALDGDGDRLMMVDKNRQVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQILNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIAAAIGGI"
     gene            complement(16169..17020)
                     /gene="folP"
                     /locus_tag="ACSIGJ_000735"
     CDS             complement(16169..17020)
                     /gene="folP"
                     /locus_tag="ACSIGJ_000735"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HHQ1329674.1"
                     /translation="MARHVWRAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRSGADFVPPEEEWARVEPVLAEAAGWGVPVSLDTRR
                     TVVMEKALALGGIDIINDVAALTDEGAVELLARQADTGICLMHMRGLPETMQDNPKYQ
                     DVVGEVARYLKTRSETCVAAGIAPQRITLDPGFGFGKNLQHNIALMRHLPELMAETGL
                     PLLIGVSRKSMIGKLTGEADAAARVHGSVAAALASVARGAQIVRVHDVKATADALKVW
                     EALGVNR"
     gene            complement(17160..18053)
                     /locus_tag="ACSIGJ_000736"
     CDS             complement(17160..18053)
                     /locus_tag="ACSIGJ_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HHQ1329675.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWSNADAVFLDPFFTSANLYDTLSADEGRGRCRRLAEQAASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLGHTKGHPYRLDVESIVKVRQISHLPVIANITGLWSRDMPQEILY
                     GLAKAAGACGIVGTCFEKAGG"
     gene            18144..20246
                     /locus_tag="ACSIGJ_000737"
     CDS             18144..20246
                     /locus_tag="ACSIGJ_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HHQ1329676.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTMLLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIRRRLLPRPTVILKNLTITEPDGGRVAVSVKETKIGLSWK
                     NLWSDRIQVEKWVVSGADLALTRDRNGAWNIQDLFDGAKHSASVNRIIVENSTVRLNF
                     LQQQLILKEISLNLQSPDSSGQQFESSGILVWRKLSVPWKSRGLFLSDGIGTPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPALALK
                     NNSIKTGTVNGTFTAGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRLQTNF
                     SLGSPLVWSRDNGLDAPRLHISTLQDTVDRLPQPRFISRLDGSLSIPNLQNWNAELNG
                     TFDRQPVAAKFKYTREGAPHLEAAAALQKLNLAPYLDEFRQQNGKIFPDILGRLSGNV
                     EAHLKIGSIQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTASGENRKQLIRSLQGSLSLNISN
                     GAWHGIDMDSILKNGLSGKISGSTPFYRFTLNNEISDGISRHIDTELFSDSLYVTSNG
                     YTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRKEKQK
                     ILEDTLLEQWQWLKPKEP"
     gene            complement(20310..21788)
                     /gene="ubiD"
                     /locus_tag="ACSIGJ_000738"
     CDS             complement(20310..21788)
                     /gene="ubiD"
                     /locus_tag="ACSIGJ_000738"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HHQ1329677.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPIKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLVMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPSTILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGSDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQGHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFSEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMTGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATSKWPGETDREWGRVIKKDPAVTVKIDGIWGKLGL"
     gene            complement(21808..22323)
                     /locus_tag="ACSIGJ_000739"
     CDS             complement(21808..22323)
                     /locus_tag="ACSIGJ_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HHQ1329678.1"
                     /translation="MHTCTSCGEKHEDMPATGFTAPYPYNQLSEEERIAYQAECDSDF
                     CIIRYSGQTDRFIRAALPIPIIGHQETLEYGVWVSVSEKSFNDYQSRFYDNPENAVYF
                     GMICNRLPPYETDTFGLHCNVVTQSDNQRPLLQLHQSGGHPPVRDFYHGMGYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22330..22867)
                     /locus_tag="ACSIGJ_000740"
                     /pseudo
     CDS             complement(22330..22867)
                     /locus_tag="ACSIGJ_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685546.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22943..23026)
                     /locus_tag="ACSIGJ_000741"
                     /pseudo
     CDS             complement(22943..23026)
                     /locus_tag="ACSIGJ_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
     gene            complement(23029..23772)
                     /locus_tag="ACSIGJ_000742"
                     /pseudo
     CDS             complement(23029..23772)
                     /locus_tag="ACSIGJ_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            23972..24400
                     /locus_tag="ACSIGJ_000743"
                     /pseudo
     CDS             23972..24400
                     /locus_tag="ACSIGJ_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677797.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioester dehydrase"
     gene            24438..25167
                     /gene="fabG"
                     /locus_tag="ACSIGJ_000744"
                     /pseudo
     CDS             24438..25167
                     /gene="fabG"
                     /locus_tag="ACSIGJ_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
     gene            25161..26506
                     /locus_tag="ACSIGJ_000745"
                     /pseudo
     CDS             25161..26506
                     /locus_tag="ACSIGJ_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257271.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase"
     gene            26560..26635
                     /locus_tag="ACSIGJ_000746"
     tRNA            26560..26635
                     /locus_tag="ACSIGJ_000746"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26593..26595,aa:Ile,seq:cat)
     gene            26704..27468
                     /locus_tag="ACSIGJ_000747"
     CDS             26704..27468
                     /locus_tag="ACSIGJ_000747"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HHQ1329679.1"
                     /translation="MIMKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSADNTA
                     EIAEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVRT
                     GDYAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKGGSVQGKVHETVQTPYPERRLK
                     HFMYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVSFVRDIILRPIWGFFKIYILNK
                     GFLDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            27469..28533
                     /locus_tag="ACSIGJ_000748"
     CDS             27469..28533
                     /locus_tag="ACSIGJ_000748"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HHQ1329680.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     DELMRRRFSEVSSVFTTRLHTFNGLFSLYALTRFIRENHISHLMIHTGKIAALSVLLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRGVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAGH
                     QCVAARFTINHTADKLLDAI"
     gene            28767..30104
                     /locus_tag="ACSIGJ_000749"
     CDS             28767..30104
                     /locus_tag="ACSIGJ_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HHQ1329681.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGKNAVDSFRVLRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAIHAGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAVRSLTLPDAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PNQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGAWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNSLGIL"
     gene            complement(30279..31291)
                     /locus_tag="ACSIGJ_000750"
                     /pseudo
     CDS             complement(30279..31291)
                     /locus_tag="ACSIGJ_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703756.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
     gene            complement(31300..32094)
                     /locus_tag="ACSIGJ_000751"
     CDS             complement(31300..32094)
                     /locus_tag="ACSIGJ_000751"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HHQ1329682.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRSWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAVVSV"
BASE COUNT         7361 a         9248 c         8443 g         7268 t
ORIGIN      
        1 caaatgggtt aaaccagtat ccatacatca gcttttttat catcctactt tttattcatc
       61 cgatcgggca gacagatttc aaagatgaaa agcctattct attcacaccc atttgatgtc
      121 atttccacac ggacgaacaa ataaattccc ataaaaaaac ggagcagata cctgccccgt
      181 tttttaattt aatccgaatt tagaattttg caccggattg gtttgccata tagtcaacag
      241 ccgctttgac ttcgtcatcg ctcaaacctg cattgccgcc tttggccggc atcgcgttaa
      301 agccttcaag ggcatgtttg tgcaaggttt ctttgccttt tttgatacgc ggtgcccaat
      361 cgtctttttt gcctatgccg ggaataccgg gaatcgaacc gccgtggcac acctgacagg
      421 ttgcttcgaa gactttttta ccgtcaacgc cgaccgcagg ggctgccgca cccttgtctt
      481 ctgccttcgc ttctgccgga gctgcactat cggcaggagc agaagctgtt cctgaagcgg
      541 cattgtcggc aggcgcagcc tcatcaggat tcgggaaaga accgccgctt ttattcgcca
      601 tgtaggtaat cgcccgtttg agttcctgat cggtcaggtc tgccgcaccg cctttggcag
      661 gcatggcgtt aaagccgttc agcgcgtgtt ggaacaaggt atcgaagcct tgcgcgatac
      721 gcggcgccca gtcgccgttg tgttccagtt tcggagcgtt cggcacattg ctgtccgccg
      781 cgtggcattg gatacagatt ttgccgaaaa tctgttcgcc ttggcgttcg ccgacgggga
      841 tgccgtcacc catcgtcaat tgtccgacag gctggatgcg ggtctgcgtt gccgcttccg
      901 tagtggcatc gacatcgccg aacgatccac tgcccgccag cttaatcagg aaataaagga
      961 ctgcaataac aataacgata ccgctcacaa gggtaaacag tgcagagcct tgggctttgt
     1021 tgtcgcggag ttgtttcatt tggtaggcct cgccgtcagg ttaggttgtg ctgtagatta
     1081 tagtttggcg tgttaaacac agttaacaat attttgctgg attatactga attcacatgg
     1141 tctttccaat cgctatcatt gaaaatatga aaaaatttgc caatggtatc tgtacaaaac
     1201 aaataatccg ttgaaaatac cggattatct ccaagaaaac tccccttgtg ccgccatacg
     1261 ccgcctgccg gcgcaagata acctttgcca atttgcagaa tttacgttaa ccttacgttt
     1321 tccgcaccca tagctcagtt ggaagagtgt cagtttccga agctggaggt cacaggttcg
     1381 atccctgttg ggtgcgccaa ttataaagag gccgtctgaa agataaatat ttttcagacg
     1441 gccttttgac ttacttcaaa ttcttatttc aacacttcgg caagcgcatc ggcgacataa
     1501 gtgatatttg atgtattcag cccggcgacg cacatcctgc cggaatccag caggtaaacg
     1561 gcaaattcgt cgcgcaacct gcggacttgc cccacgctca accccgtgta gccgaacatt
     1621 ccgcgctgtt tgatgaaata agtgaaatcg cggtcgggga ttcgggcggt caatacgccg
     1681 taaagtttct gccgcatcgc acggatgcgg tcgcgcatca tataaacttc gttttgccac
     1741 aaggcgtaaa gttcggagct gttcatcacg tcggcggcga tatacgcgcc gtgcgcgggc
     1801 gggctggagt agatgcggcg gacggtgaat ttgagttgtc cgaacaccaa atccgcttct
     1861 tctttattgg ggcaaaccac gcttaagccg ccgacgcgtt cgccgtagag cgacaggttt
     1921 tttgaaaacg aattgctgac aaacagcggc aaatccattt ccaccgcttt gcggacggcg
     1981 taggcatcgc tgtccaaatc gccgccgaag ccttggtagg cgatgtccat aaacggaatc
     2041 agtttgcgcg ttttgatgat ctgcaacact tcgtcccatt gccgttccga catatccacg
     2101 ccggtcgggt tgtggcagca gggatggagg atgaggacgc tgttttcggg cagggtgttg
     2161 aaaaacgcgg tcatttcgtc gaatttcacg ccgacagtgg cagggtcgta atatgggtaa
     2221 gtgccgacct cgaaacctgc gccttcaaaa atgccgcgat ggttgtccca agtcggatcg
     2281 ctgacgtagg cgcgcgcttc gggaaaccag cggtgcagga aatccgcccc gactttgagc
     2341 gcgcccgagc cgcccagagt ctgtacggta acgatgcgtc cctgcgcgag cgcggggttg
     2401 cctttgccga acaacaaatg ctgcaccgcg ctgcggtaaa cgtccaaacc ttccatcggc
     2461 aggtagggcg acggcgcagg cacagcggtg cgggcggttt cggcacggct tacggattcc
     2521 aatacgggca ttctgccttc gtcgtcgaaa taaatgccta tgctcaaatt gactttttcg
     2581 gggcgcgggt cgtttttgaa ggtttcgacc aaactcaaaa tcgggtcgcc gggatagtat
     2641 tcgatgtgtc ggtacatagt ccttaccttt tgcttttcaa aggattttct ttttcaacag
     2701 tacgccgctt tcgatatggt gcgtaaacgg gaattggtcg aacagggcgg cacgtccgac
     2761 cgtatgggtt tccgtcaagg tgtccaaatt ggcgcgcagc gtttcgggat tgcaggaaat
     2821 gtagatgatg ttgtcgaact gcgacaccag cttcaaagtt tcctcatcga tgccggcacg
     2881 cggcggatcg acgaaaatag tggaaaatgc gtaatccgtc aaaacaatac cgctctcctt
     2941 aaggcgtgta aactcacgtt ttccggtata ggcttcggta aactcctcag cagacaggcg
     3001 ggcgattttg atgttgccta tccagtttgc ctcaatattc cattgcgccg cactgacgga
     3061 ggttttggag atttcggttg ccaaaacctg tcggaaatat cgggacagcg gcagggtgaa
     3121 attgccgttg ccgcaataca gttcgagcag gtcgccgccc aagccttccg ccgcgcggca
     3181 cgcccattcg agcattttcc ggcatacagc ggcattcggc tgggtaaaac tgccttcgat
     3241 ttggcggtag tggaaatcgc ggtcgccgac gcgcagggtt tccgtcacat agtcctgttt
     3301 taagactatt ttctgccccc tgctccgccc gataacggaa atatccaact gttgctgtaa
     3361 cgcttgcgcc gccttcatcc actcatcatc aagccttttg tggtaaatca tggtaaccag
     3421 catttccccg ctgagcgtgg acagaaattc gacggcatac caatggtttc tcagttcggc
     3481 agattgggcg gcaacggcaa tcaattcggg catcaggcag ttgactgcct ctgaagcagc
     3541 atcgaaacgg tcgcaacgga tcaggcttgc cccgctagcc ttctgccctt tttcaaacat
     3601 ggcgtaaaac atttcaccgc cttcgtgcca aatgcggaat tcggcgcgca tgcggtagtg
     3661 tttgtccgga gattcgtaca cttcccactc aggaacatcc aaacctgcaa aaagggtttt
     3721 aaggtagtct tttttacctt gaagctgctg cgtgtagtca ttcatatcgc tatcctgaaa
     3781 aatcaggccg gattataagc cgattttcct cgccgcacgc aaactctttg actgccgccc
     3841 cttcaatgac ggacgggctt ttgtgctaaa atccgccatc tttccacact ataccgataa
     3901 agggaaaaat catggcaggc aacactttcg gacaaatctt caccgtgacc accttcggcg
     3961 aaagccacgg cgcgggtttg ggctgcatca tcgacggctg cccgcccggc ttggaattaa
     4021 gcgaagcgga tatccaattt gacctcgacc ggcgcaaacc cggcaccagc cgccacgtta
     4081 cccaacgccg cgaagccgac caagtcgaaa tcctctccgg cgtattcgaa ggcaaaacca
     4141 ccggcacacc catcgccctc ctaatccgca acaccgacca gcgcagcaaa gactacggca
     4201 acatcgccac cgccttccgc cccggacacg ccgactatac ctattggcac aaatacggta
     4261 cgcgcgacta ccggggcggc ggcaggagtt ccgcccgcga aaccgcagcc cgcgttgccg
     4321 ccggagccgt tgccaaaaaa tggctgaaag aaaaattcgg cacggaaatc accgcctacg
     4381 ttacccaagt cggcgaaaaa aaaatccggt ttgaaggcag cgaacacatt tcccaaaatc
     4441 ctttttttgc cgccaaccaa agccaaatcg ccgaactgga acactatatg gacggtgtgc
     4501 gcaaatcctt ggattccgtc ggcgcgaaac tgcatatcga agccgccaac gtccccgtcg
     4561 gtttgggcga acccgttttc gaccgcctcg atgccgaaat cgcctacgcg atgatgggca
     4621 tcaacgccgt caaaggcgtg gaaatcggcg caggttttga cagcgtaacg caacgcggca
     4681 gcgaacacgg cgacgaactg accccgcaag gcttcctgtc caaccattcg ggcggcatcc
     4741 tcggcggcat cagcaccggg caagacatct gcgtcaatat cgccatcaaa cccaccagct
     4801 ccatcgccac accgcgccga agcatcgaca ttcacggcaa ccccgtcgaa ctcgccaccc
     4861 gcggcaggca cgatccctgc gtcggcttgc gcgccgcgcc gattgccgaa gctatgctcg
     4921 ccttggtttt aatcgaccac gccctgcgcc atcgtgcgca aaatgcggat gttgccgcgg
     4981 atacgcccga tatttcccgc tcggacaagt aaaaagattt agccaaaaca cgggctttat
     5041 actaaaatac cgaccattgc cgtgccgtct gaacgcacga ttcttagagg aatacaaccg
     5101 aaatgacaca agaaaccgct ttgggcgcgg cactgaaatc cgccgtccaa actatgagca
     5161 aaaagaaaca gaccgaaatg atcgccgacc acatctacgg caaatacgat gtattcaaac
     5221 gcttcaaacc gttggcgctc ggcatcgatc aggatttgat tgccgctttg ccgcaatacg
     5281 attccgcact gattgcacgc gtcctcgcca accactgccg ccgtccgcgc tatctgaaag
     5341 ccttggcgcg cggcggcaaa cgtttcgatt tgaacaaccg tttcaaaggc gaagttaccc
     5401 ccgaagaaca ggcgattgcg caaaaccatc ctttcgtgca gcaggctttg caacagcagt
     5461 ccgcccaagc tgccgccgaa acgccgtctg ttgaagccga agcagccgaa tcttccgcag
     5521 cagaataaat ccccaaacga aatgccgtct gaaaaccgat ttggtttcag acggcatttt
     5581 ttcgtatgcg gcaatcacgg ctcaaatatc gagttgcgcc gtatcgcctt cgcgttccat
     5641 ccaagcgcgg cgggcggcgg cttcgccttt gcccatcagt ttgacgaaga tgtcgcgcgt
     5701 ctcgtcatcc gcgccttcgg ggatttgcac ctgcaacagg cggcgggtgt cggggtgcat
     5761 ggtggtgtct ttgagctggt cggggttcat ctcgcccaag cctttgaaac ggctgatgga
     5821 ataggcggtt tctttaacgc cttctttttg cagccgctct aaaatgccgt caagttcgtt
     5881 ttggtcgagg gcgtagaatt tgcgggcggg tttgcttttg ccttgtgcgt tgacatcgac
     5941 gcggaacagc ggcggctggg cgacgtagat gtgtccgtcg gcgaccagtt tggggaagtg
     6001 gcggtagaac agggtcaaca gcaaaacctg aatatgcgaa ccgtccacgt cggcatcgga
     6061 caagatggct attttgccgt agcgcaggcc gcttaagtcg ggatggtcgt tgatggcgtg
     6121 cggatcgaca ccgatggcga cggaaatgtc gtggatttcg gcgttgccga aaagctggtc
     6181 ggggtggact tcaaagctat tgagcacttt gccgcgcagg ggcaggatgg cttgtgtggc
     6241 tttgtcgcgg gcgagtttgg cggagccgcc ggcggaatcg ccttcgacga ggaagagttc
     6301 gttttcgcgg atgtcttcgc tttcacagtc ggtcagctta ccgggcagga tggcgacgcc
     6361 gctgcctttt ttcttttcga tttttttaac cgaacgtatc cgtgcctgtg cctgacggat
     6421 ggcgagttcg gcgatttttt tgccgaagtc cacgttttgg ttcagccaca attccaaagg
     6481 atcgcccgat acggcggcga caagtttcag cgcgtcgcgg ttggtcagct tgtctttggt
     6541 ctgaccttgg aattgcgggt cgaggacgcg ggcggagagg acgaaggcgg tttttccgaa
     6601 cacgtcgtcg ctttgcactt taacgccgcg cggtaagagg ttgtgcaggt tgatgaaatt
     6661 gttgacggcg ttgaacacgg cttgtttcaa gcctgcttcg tgcgttccgc ccagcgaggt
     6721 cgggataagg ttgacgtagc tttcgttggc gcacgagcct tcttccagcc aagtcaaagc
     6781 aaacgcggca ccttcgccaa tgctgaaatc gccgttgtgg ccgtctgaaa tgtagttttc
     6841 gcaggagaac agcggcacgg cttcctgtgc gtcggcaatc aggtcggtca gatagctttt
     6901 cagaccgtcg gggtaatgcc aggtttgggt gtgcgcttcg tcttcgcctt tgaccggacg
     6961 ggtcagggaa acgcgcacgc ccggcagcag cacggctttg gcgcgcaata gacgctcgag
     7021 ttcgggaatg ctgtaattcg ggctttcaaa atatttgccg tccggccaca cgcgcacttc
     7081 ggtgccgctg tctttgacgg cgcatttgcc cacttgtgcc aacggttcga ccacatcgcc
     7141 gccggcaaac acgatgcggt ggactttgcc gtctcgtttg acactgactt caagtcgggt
     7201 ggaaagcgcg ttggtaacgg atacgcccac accgtgcagg ccgcctgaaa aggcatacgc
     7261 actgccgccg tcttttttgt tgaacttgcc gcccgcgtgc aggcgggtaa acacgagttc
     7321 gactacgggt acgccttctt cagggtgcag gccgacggga atgccgcgcc cgttgtcgcg
     7381 cacggaaagc gaaccgtctt catgaatttg cacgtcgatt tcagtggcga aaccgcccaa
     7441 cgcctcgtcc gccgcgttgt cgatgacttc ttggcagata tgtgtcgggc tgtcggtgcg
     7501 ggtgtacatg ccgggacgtt ctttgaccgg ctccaagcct ttgaggacgg tgatgctgga
     7561 ttcgctgtat tggttgtttt tagccatagg aataatctga aagtaagaaa aacaacgctt
     7621 tcagacggcc tgaaagcgtt gcgttccgtt gttttaccgg ttgccggaag gttggcgggc
     7681 ggcaaagtct tcataacttt ccataccgcg caggaagcgg gaagagagtt ctttcaacgc
     7741 ccccaaataa acgtcgcgtt tgaaatcaat cacttcgtca acgggcgccc aatattgatg
     7801 ccaacgccag ccgtcaaatt cggggtggcg ggtggcgcgc aggttgacat cgcaatcccg
     7861 gccggtcagg cgcaggagat accaaatctg cttctgcccg cgataagagc cgcgccattc
     7921 gcggcgcacc cagttgttcg gcacgtcgta acgcagccag tcgcgcgtcc gcccgacgat
     7981 tttgacgtgt tgcggcagaa gtccgacttc ttcgtaaagt tcgcggtaca tcgcggtttc
     8041 ggggctttcg ccgggcttga tgccgccttg aggaaactgc catgaatgtt cgcgcacgcg
     8101 cttaccccaa aagacttcgt tacgttcgtt aattaggata ataccgacat tggggcgata
     8161 gccttccctg tccaacacgg tgtcgccctc cgttaaattc aatcttggga ttttcccaca
     8221 aatcggacgg ttttgacaaa tcggacggta tggttcaagc atgtcgaaac acggacggat
     8281 tcgggaaaat atcttaaatt tggtttacaa taatacgttt caaattaatt ccggaatcag
     8341 actatgttag atatccaatt gctccgcagc aacaccgccg ccgttgccga acggcttgca
     8401 cggcgcggtt atgactttga taccgcgcgt tttgacgcac tggaagaacg acgcaagtcc
     8461 gttcaggtga aaaccgaaga actacaagcc tcgcgcaaca gcatttccaa acaaatcggc
     8521 gcactgaaag gtcagggcaa acatgaagag gcgcaggcgg ccatggatca agtcgcccaa
     8581 atcaaaaccg atttggaaca ggctgccgcc gatttggatg ccgttcaaaa agaattggac
     8641 gcatggttgt tgagcatacc taacctgccg cacgaaagcg tacctcccgg taaagacgaa
     8701 accgaaaacg tcgaagtccg caaagtcggt acgccgcgcg aatttgattt tgaaatcaaa
     8761 gatcatgtcg atttgggcga gcctttgggc ttggatttcg aaggcggcgc gaaactctcc
     8821 ggcgcacgat ttaccgtgat gcgcggacaa atcgcccgtc tgcaccgcgc cttggcgcaa
     8881 ttcatgctcg atacgcacac gctgcaacac ggctacaccg agcattacac gccttatatc
     8941 gttgacgata cgacgctgca aggcacaggt cagctgccca aatttgcaga agatttgttc
     9001 cacgttaccc gcggcggcga tgaaaccaaa accacgcaat atctgattcc gacagccgaa
     9061 gttaccctga ccaataccgt tgccggcagc attatcccgt ccgaacaact gccgctgaag
     9121 ctgaccgcgc attcgccctg tttccgcagt gaagcgggat catacggcaa agacacgcgc
     9181 ggcctaatcc gccagcacca gttcgacaaa gtagaaatgg tacaaatcgt tcatcccgaa
     9241 aaatcatacg gcgcgctgga agaaatggtc ggccatgccg aaaacatcct gaaggctttg
     9301 gaactgccct accgcgtgat taccctgtgt accggcgaca tgggtttcag cgcggccaaa
     9361 acgtatgact tggaagtttg ggttcccgca caaaatacct accgcgaaat ctcaagctgc
     9421 tccaactgcg aagatttcca agcacgccgc atgaaggcgc gtttcaaaga cgaaaacggc
     9481 aaaaaccgct tggtacatac cttaaacggc tccggtttgg cagtcggtcg cactttggtc
     9541 gcggttttgg aaaaccatca aaacgccgac ggcagcatca atatccccgc cgcactgcaa
     9601 ccgtatatgg gcggtgttac caagttggaa gtgaaataag ccttaaccct gaacataaat
     9661 gccgtctgaa acctgtttca gacggcattt cctttaaact cttaaaacac atcaaccgtc
     9721 ggcacgaacc gcattgccgc aatcgccggt ctgtccgacc tcgcggatat tggacagcgt
     9781 aacttccgaa atattaccca acgcctcttc cgtcaaaaat gcctgatgtc cggtaaacag
     9841 tacgttatga caagacgaca ggcggcggaa cacgtcgtcg gtaatcacat cgttggattt
     9901 gtcttcaaaa aacagctcgc gctcgttctc gtacacatcc atgcccaatg caccgatttt
     9961 ccggcatttc aacgcctcga tggcggcggc gctgtcaatc agcccgcccc ggctggtgtt
    10021 gataatcatc acgccgtctt tcattttgtc gaacgccgct tcgttcagca tatagtggtt
    10081 ttccggcgtg gcgggacaat gcagcgtgat gatgtccgat cgggcataca gctcgtccaa
    10141 atccacatac ctgccgccga gtttttccac ttcggggttg caaaacggat cgtaagccag
    10201 caggttcata ccgaaaccct ttaaaatccg catcgttgcg ataccgattt tccccgtgcc
    10261 gataacgccc gccgttttgc cgtacatatt gaagccggtc agaccttcca gcgaaaaatt
    10321 cgcatcgcgg gtacgctggt aggctttgtg gatacggcgg ttcaaagtca gcatcagacc
    10381 gaccgtatgt tccgcaaccg attcgggcga ataggcaggc acgcgcacga ctttcaagcc
    10441 caactcttca gccgccttca aatccacatt attgaagccg gcacaacgca acgccacagt
    10501 tttcacgcca atttgcgcca atttttccaa cacgggccgg ctggcgtcgt cgtttacaaa
    10561 aatacaggcc gcttccgcgc cttccgccat tttcgccgtt ttcgcatcca gcatgaaatc
    10621 aaaaaactcc agctcgaggc cgaaatgccg gttggcgcgg gtaaaatgtt cgcggtcata
    10681 gcttttcgta ccgtaaatcg caatcttcat caatatgtcc agttgtcgtc tatggttgag
    10741 aaacggcata atacaccgaa ttcaaacaaa tcagtagaat atggcggatt aaaattgatt
    10801 tcatgcacgg catttccatt tcaaaacaca aaactcaatc gcccatcgcc gccagaagct
    10861 ccgcctgatg ttcggcaatc agggcatcgg tgatttctgc caaatcgccg tccatcacaa
    10921 aatccagctt gtgcagggtg aggttgatgc ggtggtctgt taccctgcct tggggatagt
    10981 tgtaggtgcg gatgcgttcg ctgcggtcgc cgctgccgat gagggatttg cgttcggcgg
    11041 cttctttggc ttggacttcg cgtttttgcg cgtcgttcag gcgggcggcg aggactttca
    11101 tcgcctgcgc tttgttggcg tgttggctgc ggccgtcttg gcattcgacc accatgcctg
    11161 tcggcaagtg ggtaatgcgg acggcggagt cggttttgtt aatgtgctga ccgcccgcgc
    11221 cggatgcgcg gaaggtatcg gtgcgcaggt cggcggggtt cagttcgatg tcttcgagct
    11281 cgtccgcttc gggcatgacg gcaacggtac aggcggaggt gtggatgcgg ccttggcttt
    11341 cggtggcggg gacgcgctgc acgcggtgtc cgcccgattc aaatttcaga cggctgtacg
    11401 cgccgagtcc gacaatacgg gcgatgactt ctttatagcc gcccaattcg ctttcgttgg
    11461 cggacacgat ttcaacctgc cagcggttgc gttcggcgta gcggctgtac atacgcagca
    11521 aatcgccggc aaacagcgcg gcttcgccgc cgcccgtgcc ggcgcgtatt tcgataaaga
    11581 tgtttttgtc gtcgtcggca tctttgggca gcagcagttt ttgcaattcg gtatcgagtt
    11641 cgtcgatttt ggctttcgcc gcttcgattt cttcggcggc aaagtctttc atttcggggt
    11701 cggacaacat ttcttcggca tccgccaagt cgctttgggc aagccgatag ttttggaata
    11761 cttcgacgac gggggtcagt tcggcgtgtt cgcgcgtgag cttgcggtag ttgtccatat
    11821 cggatgtggc ttcgggttgc cctaaaaggt gggtaacttc ttccaaccgg tcgccgagtt
    11881 gttgcagttt ttctaagata gacggcttca taattcttcc ataacaaacg ccgcctgaat
    11941 ttcagacggc atcaaaacgc ggtcattata atatattttc tgaatattca aaaagataat
    12001 ctagggacgg agcagactgt cggcattgcg ttccgttatc gcggcgattt ccgccgcgcc
    12061 ggtaccgcgt atttccgcag caatttcggc aatccgaaga atgttggcag gcgtattgac
    12121 ggtgttttcc aacatgaacg ggctatccgt ttccaatacg aaatccccgt cgttcaaggc
    12181 tttaagcgta tcgcgcactt tacgcgcgtt cggattgagc agcagcgaac cgatgccgat
    12241 tttgaacccc agttttgtca acacgcgcgc ttcttccgcg ctgcctgaga aagcgtgaac
    12301 gatgccgcct tgagtaaagc ctgtctgttt gacggcggcg gcgatgtcgg cggtggcttt
    12361 gagattatgg ataatcacgc ggcggcgcag ggtttgcgcg atggcaagct ggcggctaaa
    12421 aacttgaatt tgccgttcac gctgcggcgg cgtttgggtt ttgtcgtaaa aatccaagcc
    12481 gatttcgccg acccacgctc gggggtaacg caccaatacg gcttccaaac cggaaaaatc
    12541 ctgttccgca atgccgtctg aaaaccaagg atggatgccc agcgcaatac ggatttgacc
    12601 gtgttcggac ggcatttccg acagttccgc cacgtcctgc caatcctgcg gacaggtagc
    12661 gggaacgata aaccgccaca cgcccgcctg tcgcgcttcg gcaagcacct gcgccagatt
    12721 gccgcgcaaa acggaatcgg caagatggca atgtgtgtcg gtcagcctca tcttcacacc
    12781 agccccaacc gctgcaactc gtgtttcacc ccgtccgcgc cccatccttc ggaaacggca
    12841 agcgtcatca atgcggttgc ggtttccaaa ttgcatttgc cgccgttgac cacgcccgcc
    12901 cgacgcagcg cgtcgccctg tgcgtaaacg gcggcagcgc aaccctgcgg cacttggctg
    12961 atgttaagca acaatttgcc ttgctgcgta aagtcttgca cggcgcggat gaagccttcg
    13021 ccggcggagg tattgccgtg tccgtagctt tgcaggataa gggcttgggc gggaagctgt
    13081 ccgagcccgt cggcaagttc ttggacggca aagccgggga taagcgtgcg gacggcgatt
    13141 tttgcctgcg ggtcgggata acggatttcg aggccgtctg aaacatctgc cgcgtcctga
    13201 ttcgggatgc ggacattgtg ccagccccgg gtttcgtccc actcggcaag tgcgccgaaa
    13261 tgggcattgc cgaaaccggc ggcggtttcc gtgctgattt tgctgctgcc gacggcggga
    13321 tacagtttgc cgtcaaaggc aataagcgtc tgcttcaatc cgagtgggaa tgcggcaacg
    13381 gcagtcacca gattgcgcgg agcgtcgctg ccctctgcgt tgtacggcca ttgcgaaccg
    13441 gtcaggacga tcggtttgtc caaaccttgc agggcgaggg cgaacatatt ggcggtatag
    13501 gcgagtgtgt ccgtgccgtg cagcaccaaa acaccgtcgt actgcggcag cgcggcggca
    13561 atgatgtcca gccaagcgca ccagttttgc agttggacgg cggaagaatc aatcagcggc
    13621 tcacaaacgt gccaatcgaa atccatgccg tctgaaaacg gcgcgagtgc ctgcccgacc
    13681 agcgcggtat cggggcgcaa gcccgcactg ctttgcgtca tgccgatggt gccgccggta
    13741 taaaggacaa aaattttctg tttcatggct cattctgaaa atgcaaagac cgcctgaaac
    13801 gcgaaatgcg tccggacggt cgaaaatccg atttactgct gcttctgtgc cgcttttttc
    13861 gctgcctttt ccgccttgcg tttggcgcgt ttttcgctca attgtgcgcg gtaaagctga
    13921 taatgtcggc gtttgcgcca ccagaaccac gccagcgccg ccgccagcac gcccaatgcg
    13981 atgaagatgc ccgattgcag gctgtgcatt ttcgccatca gccaatcgat gttgtgcgcg
    14041 ccgtactcgc ccaagtaaat ccaaacgggc acggaaatca gcgcggccag cccgtccata
    14101 atcagaaagc gcagatacga tactttgcgg ctgatgccgg cggtaacgaa aacggcagtc
    14161 cgcaaacccg gcaggaaacg ggcgacaaac agaacccagt tgccgtattt gtcgaatttt
    14221 tcctgaacct gcgcgtaacg tttcggcgtc atgatgcgcg caatcggttt gaacttgagg
    14281 attttctgcc cccagatgcg tccggcggca aacatcacgc cgtcgcccgc caacacgccg
    14341 agcataccga ccgcaaacat aatatgcgga ttggtataac ccatacccga aatcacgccg
    14401 cccgttacca aggtcaaatc ttcgggaatc ggcacgccga aaccgcagat gaccaaaacg
    14461 aaaaacacgg ccgcatagcc gtattcgaca aaaaaggctt ccaaaagggc aaacatagcg
    14521 gatattccat tgtcggagat aaaaagtcag aacaagccga aacattttct gcgcgaaacg
    14581 gcattctatc aaagattatg ccgtctgaaa gcggcagggg gggcagattc gcgccccgcc
    14641 ggacgttcag acggcattta aacaggaaaa ccgccgctgt tgaaacggtt ttgcctgatt
    14701 ttgcctaaat gccgccgatg gcggcggcga tacgctccgc cccgtcgcgc gcccaatcgg
    14761 cctgacgcgc ctcgaccatc acgcgcacga cgggttcggt tcccgaagcg cgcaacacga
    14821 cacgcccttt gccttcgagt tctttttcca cttcggccaa cacgtctttc gaagcctcct
    14881 gccatttctg acccttttgg atgcgcacgt taatcatcgt ttgcggatac ggctgccagt
    14941 cggcgcaaac ggtggcgagg tcttggttca ggatttgcag cgccgccaag acttgcagcg
    15001 cggaaataat gccgtcgccg gtgttatgtt tgtccatgca caaaatatgg ccgctggctt
    15061 cgccgccgat gagccagccg cgttggttca gctgttccaa cacatagcgg tcgccgactt
    15121 tggcgcggca gaaatcgacg ccctgctctt tcagggcgat ttccatcgcc atattggtca
    15181 tgaccgttcc gaccacgccg ccgatgttga taccttctcg ggcgcgggct ttggcgatga
    15241 cgtaaatcag gctgtcgccg tcgtaaacct gcctgttttt atcgaccatc atcaggcggt
    15301 cgccatcgcc gtccaaggca ataccgtaat cggcttcgtt ttgcaacacg gcggcttgga
    15361 gggttttggt atgggtcgcg ccgcattttt cgttgatgtt gtagccgttg ggttcgttgc
    15421 cgatgctgac gacctgtgcg ccgagttcgt ggaacacttt gggtgcaacg ccgtatcctg
    15481 caccgttggc ggtatcgata accaacttca aaccccgaag gtcggaatgg ccggggaagg
    15541 tggatttgca aaactcgata tagcggtcgt ccgcaccgct gatgcggcgg gcgcgtccga
    15601 gacgtgcgga cggttgggtt ttcatttctt catcgatttt ggcttcgatt tccaactcga
    15661 cttcatcgga aagtttcacg ccgccttcgg caaagaattt gatgccgttg tcggaatagg
    15721 tgttgtgcga cgcggaaatc atcacgccgg cggacaggcg caatgcgcgg gtcagataag
    15781 ccacaccggg cgtgggcaac ggcccggtct gtaccacatt cacacccgcc gccgtaaagc
    15841 ccgctaccag cgcggcttcc agcatatagc ctgaaatgcg cgtgtctttg ccgatgagga
    15901 cggtcggttt ctgatcggta tcgtgctgca ccagcacctg ccccgccgca tagccgagtt
    15961 tcaatacaaa atcgggcgta atcggaaact gaccgacttc gccgcgcacg ccgtccgtgc
    16021 cgaaatattt ttttgccatc atttgctccg tgaatgtgaa ccgttgtccg agattataca
    16081 gtcagtttgc gccctgctgc ctgcaccgtt gatgccgtct gaagccgtcc cgcccttttc
    16141 agacggcatt cggcgcaaaa accgccgttt accggttcac gcccaacgct tcccacacct
    16201 tcaacgcgtc cgccgtcgcc ttcacatcgt gcacgcgcac gatttgcgct ccgcgtgcca
    16261 cggaagccaa agccgctgcc acgctgccgt gtacgcgcgc cgccgcgtcc gcctcaccgg
    16321 tcagcttacc tatcatgctt ttgcgcgaca caccgatcag cagcggcaaa ccggtttccg
    16381 ccatcaattc gggcaaatgc cgcatcagtg cgatattgtg ttgcaggttc ttgccgaagc
    16441 cgaaacccgg gtcgagcgta atccgttgcg gcgcgatgcc tgccgcgaca caggtttctg
    16501 accgtgtttt cagataacgt gccacttcgc cgaccacatc ctgatatttc ggattgtcct
    16561 gcatggtttc gggcaagccc cgcatatgca tcaggcaaat gcccgtgtcc gcctggcgcg
    16621 ccagcaattc gaccgcacct tcgtcggtca acgccgccac atcattgata atatcgatgc
    16681 cgccgagtgc caacgccttt tccataacca ccgtgcggcg cgtgtccaaa ctgacgggaa
    16741 cgccccaccc cgccgcttcc gccaatacag gctcaacccg cgcccattct tcttcgggcg
    16801 gaacgaaatc cgcacccgac cgcgtcgatt cgccgccgat gtcgagaatg tccgcaccct
    16861 ctttcaaaag ccgttcggca tgcgccaaag ctgtttgggc gttttgcgaa tacgcgccgc
    16921 cgtcggaaaa agaatcgggc gtgagattca cgatgcccat gattttcggt ttgtccaaac
    16981 cgatttcaaa ccgtcctgcc cgccaaacgt gtcgtgccat ctgaactcct cccgaaataa
    17041 aaaacagatt atatgccgtc tgaaaccgtc ttgtgcgctt cggacggcac cgctattcgg
    17101 gcggcagacg gcatgttgtc ccaatgtctg cgccgccttt gaatctgccg gcatgcctgc
    17161 tatccgcccg ccttttcaaa acaggttccg acgattccgc acgcgcctgc cgcctttgcc
    17221 aagccgtaca ggatttcctg cggcatatcg cggctccata atcccgtaat attcgcaatc
    17281 acgggcagat ggctgatttg gcggactttc acgatggatt cgacatccaa acggtaggga
    17341 tggcctttgg tatggcccaa cacgcccgac tcgcccagaa tcaggtggcg gttgccgcgc
    17401 gagacgacat attctgcggc attcaaccaa tcttcggcag aatggtgctt gtctttacac
    17461 agaaccaagg gaatgttcag gcttccggct tcattcaata cggcaagatc ggacatcagc
    17521 ccgccgccca aatacaggat gtccgctccc gcattcaaag ccgcttcgac atggcggacg
    17581 ttgcgaacgc gcaccaatac gggtttccct gcgccatgcg ccgatgcggc ctgttccgcc
    17641 aaccgtctgc accgtccccg cccttcatcc gcacttaaag tgtcgtataa gtttgccgaa
    17701 gtgaaaaacg gatccagaaa cactgcatcc gcattgctcc atactgacgg ttctgcagcg
    17761 atacggacgg tttccccgcc gccgaaagcc acgcctttgg cggcaacgcg gctgtcttcc
    17821 gcccgatttt cccgactgac ggttttccat gtgtccaaaa tgcggacggc tttctcgacc
    17881 tccggcagcg tctgtacctc cctgacgctc aaaaccctat cgtcgccgat tgcgccgatg
    17941 acggtacgct cgtcgccgtg agaaatgtgt tctcgcagac ctctgctgcg gatgaaggcg
    18001 acaacgccgg caatgtccgc ttcggcggca cgcctgctca tgacaataat catatttcct
    18061 cctgacacaa gagacggccc gcccaaaata ggatttttgc aagccgtgtt atactgtggc
    18121 gtgttttaca gattgttcgg gctatggatt tattatcggt tttccacaaa taccgtctga
    18181 aatatgcggt ggcggtgctg acgatgctgc ttttggcggc agtcgggctg cacgcttccg
    18241 tatatcgcac cttcacgccc gaaaacatcc gcagccgcct ccaacaaagc attgcccata
    18301 cccaccggaa aatctcgttt gatgcggata tacggcgcag gcttctgccc cgcccgaccg
    18361 tcatcctgaa aaacctgacc attaccgaac ccgacggcgg ccgggtcgcc gtttccgtca
    18421 aagaaaccaa aatcggattg agctggaaaa acctgtggtc ggatcggata caggttgaaa
    18481 aatgggtggt ttcgggtgcg gatcttgccc tgacgcgcga cagaaacggc gcttggaaca
    18541 tccaagacct gttcgacggc gcgaaacact ccgcctcagt caaccgcatt atcgtcgaaa
    18601 acagcaccgt ccgcctcaat ttcctgcagc aacagcttat cctgaaggaa atcagcctca
    18661 acctgcaatc ccccgattcg tcggggcagc agtttgaaag ttcgggcata ctggtttgga
    18721 gaaagctgtc cgtcccgtgg aaaagcaggg ggctgttcct ttcagacggc atcggcacgc
    18781 ccgaaatctc accgttccat tttgaagctt ccacttcgct ggacggacac ggcatcacca
    18841 tttccaccac cggcagccct tctgtccgct tcaacgccgg cggagcggat gccgccggcc
    18901 tcggcctgcg tgcagacact tccttccgca acctccacct gaccgcgcaa atccccgcac
    18961 tggcactcaa aaacaacagc atcaaaaccg gcacggtcaa cggcacgttt accgccggcg
    19021 gcgaatatgc ccgatgggac ggttcgttca aactcgacaa agccaacctg cactccggca
    19081 tcgccaacat cggcaacgcc gaaatctccg gcagcttcaa aacaccgcgc cttcaaacca
    19141 atttctccct cggctcgccg ttggtttgga gtcgggacaa cgggctggac gccccgcgcc
    19201 tgcacatatc gacccttcag gataccgtcg accgcctgcc gcaaccccgt ttcatcagcc
    19261 ggctcgacgg ttcgctgtcc ataccgaatc tgcaaaattg gaatgccgaa ttaaacggca
    19321 cattcgaccg ccaacccgtt gccgcaaaat tcaaatatac gcgggaaggc gcaccgcacc
    19381 tggaagccgc cgccgcgctg caaaaattaa acctcgcccc ctatcttgac gaatttcggc
    19441 aacaaaacgg caaaatattc cccgacatcc tcggcaggct gtccggcaac gtcgaggcac
    19501 acctcaaaat cggcagcatc caactccccg gcttgcaact ggacgatatg gaaacctacc
    19561 tccacgccga caaagaccat atcgcgctca gccgtttcaa gtcagggctt tacggcggcc
    19621 ataccgaagg cggcatcagc atcgccaaca cccgtcccgc cacttaccgc ctgcaacaga
    19681 atgcaagcaa catccaaatc caaccgctgc tgcaagacct gttcggcttc cacagcttca
    19741 gcggcaacgg cgatgcggtc atcgacctga ccgcaagcgg cgaaaaccgc aaacagctta
    19801 tccgctcgct gcaaggcagc ctgtcgctga atatttccaa cggcgcgtgg cacggcatcg
    19861 atatggacag cattttaaaa aacggccttt ccggaaaaat ctcgggcagc acacccttct
    19921 accgattcac gctcaacaac gaaatttcag acggcatcag ccgccacatc gataccgaac
    19981 tcttctccga cagcctctat gttaccagca acggctatac caatctggat acgcaggaat
    20041 tgtctgaaga tgtccttatc cgcaacgccg tccatccgaa aaacaaaccg attcccctga
    20101 aaatcaccgg tacggtggac aagccgtcca ttaccgtcga ttacggcagg ctgaccggcg
    20161 gcatcaattc gcgcaaagag aaacagaaaa tcctcgaaga caccctgctg gaacaatggc
    20221 agtggctcaa acctaaagaa ccgtaaatac cctgcgtaca aaaatgccgt ctgaaacaca
    20281 ttaatcttca gacggcatga ccgtaaaacc tacaacccca atttccccca aatcccatca
    20341 attttaaccg taaccgcagg gtcttttttg attacccgtc cccattcgcg gtcggtttct
    20401 cccggccatt tgctggtcgc atccaaaccc attttgccgc cgagtccgct gacggggctg
    20461 gcgaagtcga ggtagtcgat gggcgtgttt tccaccaaaa cggtgtcgcg gacggggtcc
    20521 atgcgcgtgg ttaccgccca gatgacttct ttccagtcgc gcacgtttac atcgtcatcc
    20581 accacgatga tgaatttggt gtacataaac tggcgcagga acgaccagca gcccgtcatc
    20641 acgcgcttgg cgtgtccggc gtactgtttt ttcatgctga ccaccgccat gcggtaggaa
    20701 cagccttcgg gcggcaggta gaaatcggtg atttcggaga actgcttttg caaaagcggt
    20761 acgaacactt cgttcaacgc cacgcccaaa acggcaggtt cgtcgggcgg tttgcctgtg
    20821 taggtagagt ggtaaatcgg gttttcgcgc atggtgatgc gttcgaccgt aaacacaggg
    20881 aaatggccct gctcgttgta atagcccgtg tggtcgccgt atgggccttc caacgcggtt
    20941 tcgtttggat gaatcacgcc ttccaataca atttcagcac gggcaggcac ttgcaaatcg
    21001 ctgccgatac atttcaccag ctccgtccgc gaaccgcgca gcagtccggc aaactggtat
    21061 tcgctcaaag tatcgggaac gggcgttacc gcgcccaaaa tggtggaggg gtcgcaaccg
    21121 agtacgacgg cgacgggata cggcgtatcg ggattgagtt tgcggaattc ctgataatcc
    21181 aacgcgccgc cgcgatgcga cagccagcgc ataaccagct tgtttttgcc gatgagttgt
    21241 tggcggtaaa tgccgagatt ttggcgtttt ttgtgcggcc cgcgcgtgac ggtcaagccc
    21301 cacgttacca gcggcgcaac gtcttccggc cagcaatgtt gaatcggaag ctgatacaaa
    21361 tcaacgtctt ctccttccca cacgatttcc tgacacggcg cgtttttcac cacgttcggc
    21421 gccatgctcc aaatatcttt caacagcggc agtttggaaa acgcgtcttt gatgcctttg
    21481 ggcggttcgg gttctttcaa atacgccagc gtctgcccga tttcgcgcag cttggacacg
    21541 ctgtccgcgc ccatgcccat cgccacacgt tcgggggtgc cgaacaggtt cgccaacacg
    21601 ggataatcat agcgcgtacc gtcgggctta atcgggtttt caaacaacaa cgccggccct
    21661 tcggcgcgca acacgcggtc ggcaatttcg gtcatttcca aatgcgggga aacggggtgg
    21721 gcgacgcgct tgagcttgcc ctgctgctcg agcatagcga tgaagtcgcg caggtctttg
    21781 tatttcatgt ttatcctttt tgtcctttta tcctgaacaa tccgattcgg ataccgcccc
    21841 tatccttgcc tgcgcttcgg catatcccat gccgtgataa aagtcgcgta ccggcggatg
    21901 tccgccgctt tgatgaagtt gcagcaaagg gcgttggttg tcggactggg taacgacatt
    21961 gcagtgcaga ccgaaggtgt cggtttcata agggggcagc cgattgcaaa tcataccgaa
    22021 ataaacggcg ttttcagggt tgtcgtaaaa gcggctttga tagtcgttaa aactcttttc
    22081 gctgacggac acccacacgc cgtattccag cgtttcctga tgcccgataa tcggaatagg
    22141 cagcgccgcg cggataaagc ggtcggtttg gccggaatag cggatgatgc agaaatcaga
    22201 atcgcattcc gcttgataag caatgcgttc ttcttcactg agttgattat agggataggg
    22261 ggcggtaaag ccggttgcgg gcatatcttc atgtttttcg ccgcaggaag tgcaagtgtg
    22321 cataaggttt cagactttca acacaagttt gcggtaaagc cattcgccgg caaacagcat
    22381 ccccatcaat acataggcaa tcacgcctgt ataaaccgcc caccaatcat atcgccccaa
    22441 ccgggccaac aaagcggcaa gcgtcccgtt ggcaataaag aatacgcacc aaacctgcgt
    22501 tacccggcgg gtatagcgca cggctttttc aggcaggtcg ggctgttgca gccgcgcaag
    22561 tttttcaatc accgtctgcc cggcaaacag gctgctgccg aacaccgcca acatcatcaa
    22621 attaacgagg acaggatacc aatacattga atcacgccgc ccgaacacca atactgcggc
    22681 aaaaaacagt gtaatatagt ggattaaatt taaatcagga caaggcggcg aagccgcaga
    22741 cagtacaaat agtacggcaa ggcgaggcaa cgccgtaccg gtttaaattt aatccactat
    22801 accacaaagc gggataggca atgcttaata cggtcaaaaa aatatgtccg aaaaaaccgg
    22861 gtttcatttt gaatccgcac aaacgttttc agacggcatc cgataaaaac atgccgtctg
    22921 aaaaataatt agaaataccc gattagccgg cctgaatctt caataccgcc tgtaccacgt
    22981 cgttgacggt gcggacattg cggaaatctt cagcctgcag cttgcgggct gccacaacca
    23041 ttcacgattg cgatataccc ggcggcattc ccgactgccg ttcagcagaa aacgcaccca
    23101 ttccaaaccg ctccaatatg cctcgggcag cataccggct tcagacggca tatcgtccga
    23161 agcagacaaa gtcaggctgt aacgcgtccc tttggtcaga accatcgcca aagcataagc
    23221 aaacggcgcg cgcgttgccg atacggcata tccttccggc agcggatcgt ccgccgccaa
    23281 aaccaaaacc gacccgcatc cctcttccaa cagggacgcc gcttccgcca atgccgtttc
    23341 cacaccgtcg gcacacacgg ccaatgccgt ctgctcgctc atatcccgcc tcaatatcga
    23401 ccattgcccc gccgtcgcat tgtgcaccga caaaccgaac gaagtcggcg acacggtatg
    23461 cgatttcaac agttccaacc acaaatcgaa actacgcgcc atttccccgt cgtgcgaggc
    23521 ataaactacc ggactgccgg gatgggcgga ggcaatgtcc caagccgcgt cgcataccaa
    23581 acgcgccgcc ttactcaaac ggcggcgccg catagcgggc aggaacggca attcccgcct
    23641 gacatcgggc aaaccgtcgg caaaatccgg acattccgcc cactgcctct actgggccat
    23701 atcgcgcatt ttgccgcccg aaacccgcca gtcggcgatg tcgaagtgga accggcaaat
    23761 aatcggcggc atagtttctt tcaaaaattt acactgtgcc gcattctaac caaagcccat
    23821 ccccctgaca atgccgaaat tcaaacgcat ccacgccatt ttttccgaca acgccgcccc
    23881 tcggcaaacc gcccgaatta gcctgaattt acatttatca ttataatgcc cgattaccga
    23941 caccgcctcc ctgctgccgc acagcgggcg tatggttctg atagaccgca ttacccgata
    24001 cggcgatgat tttgtcgaag caggggcgca ggtaaaaccc gaccacatcc tgctggccgg
    24061 cggcatgctg ccctatacgg catttatcga actgatggcg taggctgtcg gcgcatatgc
    24121 cggcctccaa gcccgaaaaa aggcgcaacc ggtccggctc ggttttcctg cccggcacgc
    24181 gcaaacttga aatttttacc caatccgtcc ctgtcggaac acatttgccg gcaacggcgc
    24241 atatgtctat ccaagatgcc ggggtatggg cgtgtccgac tgcgaactgc gttggacgga
    24301 cgcgccggaa accttgtctg aaacactcct ttcagacggc attttggcgc gcgcctcact
    24361 caacgtgtac agccccgaac accctgtcga aacaacttaa tgccgtctga acaggcacgc
    24421 acatacggag aacaaccatg actgaaaccg tcctgattac cggctcaaac agaggcatag
    24481 gcaaagccgc cgcactcggt tttggcggac gacggctttg atatcgccgt ccactgccgc
    24541 agccgccgcg acgaagccga agccgtggcg gaagaaatcc gcgctttggg cagaaatgcg
    24601 cgcgtgttgc agtttgacgt gtccgaccgc gaagcctgcc gcgagattct gaccgccgac
    24661 atcgaagcaa acggcgcgta ttacggcgtg gtgttgaacg ccggcctgac gcgcgacaat
    24721 gccttccccg cgttttcaga cgacgattgg gatgtggtgc cgcggactaa tttggacggt
    24781 ttttacaatg tattgcatcc gctggttatg ccgatgatac gccgccgcaa agccggacgg
    24841 attgtgtgta tggcatcggt gtccggcctg acgggcaacc ggggacaggt caattacagc
    24901 gcgtcaaaag cgggcattat cggcgcggca aaagccttgg cggtcgaact ggcgaaacgc
    24961 aaaatcaccg tcaactgcgt ggcgccgggt ttgatcgata cggagattgt cgatgaaaac
    25021 gtacccgtcg gagaaatttt gaaagccgtc cccgccgcgc gtatgggact gccggaagaa
    25081 gtggcgcacg cagtgcactt cctgatggat gaaaaagcgg cgtatatcac gcgccaggtg
    25141 attgcggtga acggaggttt gtgttgaata ccagaagggt cgcagtaacg ggcataggcg
    25201 gcattaccgc cttcggccgg gattggcaaa gcatacaggc agcattcaaa gccgaaaaaa
    25261 acgccgtcaa atatatggat tggcgcgaac gtttccccga attggaagcg caactgggtg
    25321 cgccgattga ggattacgcg ccgccgaaac attggacgcg caagcggctc agaagtatgg
    25381 ggcgcgtgtc gtacctgtgc gtcgatgcgg cggagcaggc tttgatggat gcaggtttgc
    25441 tcggggacga aagcattacc gacggacgga tgggcgttgc ctgcggctct tccggcggcg
    25501 gcaccaaaga catcggcgat gtgggcgaat tgttgctgac cggcacgtcg cgcaacttcg
    25561 gcgccaatac ctatgtgcgt atgatgccgc acaccgccga cgccaatatc ggcatctttt
    25621 tcggattaat aaggacgcat catcccgaca tcgagtgcgt gttcttcggg cagccaaggc
    25681 ataggttatg cttacgaggc catcaaatac ggtctgaccg atataatgct ggcgggcggc
    25741 ggcgaagaat tttgcccgtc cgaagtgtat gttttcgact cgctctatgc cgccagccgc
    25801 cgcaacggcg aaccggaaga gaccccgcgt ccatacgacg cgaaccgcga cgggctggtc
    25861 atcggagaaa aggcgcgggg attttcgtat tggaagaatt ggaacacgcc aaacggcgcg
    25921 gtgcgaaaat ttacgccgaa ctcgtcggct acggcgccaa cagcgacggc atcaatatcg
    25981 gcgaaggcgc agccgtattc atcatgacgc gcgatgcgga tttttccggc ggtatgcagc
    26041 ttctgggtta cggcgcaagc agcgatgcct accatatttc cacgccccgc cccgacgcaa
    26101 aggcgcaatc ctcgcctttc agacggcatt gcagcacgcc ggccttgcgc ccgaagacat
    26161 cggccggatt aatctgcacg gcaccgggac gcaccacaac gacagtatgg aaagccgcgc
    26221 cgttgcagcg gttttcggca acaatacgcc ctgcacgtcc gccaagccgc aaaccggaca
    26281 cacgctgggc gcggcgggcg caatcgaagc cgcgttcgcg tggggcattg ccgaccggca
    26341 aagcaatccc gaagggaaac ttccgccccg gctttgggac gggcagaacg accccgacct
    26401 gcccgccatt aacctgaccg gcgacggctg ggaaaccgaa aaacgcattg ccgccagctc
    26461 gtcgtttgcc ttcgggggaa gcaactgcgt cttaatcatc ggatgaaata agtttgtcaa
    26521 tcccaccgct atgctataca atacgcgcct actcttgacg ggtctgtagc tcaggggtta
    26581 gagcagggga ctcataatcc cttggtcgtg ggttcgaacc ccaccggacc caccaattcc
    26641 caagcccgga cgtatgtttg ggcttttttg ccgccctgtg aaaccaaaat gctttgagaa
    26701 accttgataa tgaaaaaagt cagcgtattg attgttgcca aaaacgaagc aaaccacatt
    26761 cgggaatgta ttgaaagttg ccgtttcgat aaagaagtta tcgttatcga cgaccatagc
    26821 gccgacaaca ctgccgaaat tgccgagggt ttgggtgcaa aagtcttcag acggcatttg
    26881 aacggggatt tcggagcgca aaaaacattt gccatcgaac aggcaggcgg agaatgggtt
    26941 ttcctgattg atgcagacga acgctgcacg ccggaactat ctgatgaaat ctcaaaaatt
    27001 gtccgaaccg gcgattatgc cgcctatttt gtcgaacgcc gcaacctttt ccccaaccat
    27061 cccgccacac acggcgcgat gcgtcccgac agcgtatgcc gtctgatgcc gaaaaaaggc
    27121 ggttcggtgc aaggcaaagt acacgaaacc gtacagaccc cctaccccga acgccgtctg
    27181 aaacatttta tgtaccatta cacgtacgac aactgggaac aatatttcaa caagttcaac
    27241 aaatatactt ccatctcggc cgagaaatac cgggagcagg gaaagcccgt gagtttcgtt
    27301 agggacatta tcctccgtcc gatttggggt tttttcaaaa tttatatcct gaacaaaggg
    27361 tttcttgatg gaaaaatggg ttggattatg tccgtcaacc acagctatta cacgatgatt
    27421 aaatatgtca aactatatta tctgtacaaa tccggcggaa aattttaaat ggaaaaagaa
    27481 ttcaggatat taaatatcgt atcggccaag atttggggtg ggggcgaaca atatgtctat
    27541 gatgtttcaa aagcattggg acttcggggc tgcaccatgt ttaccgccgt caataaaaat
    27601 gatgaattga tgcgcagacg cttttccgaa gtttcttccg ttttcacaac gcgccttcac
    27661 acgttcaacg ggctgttttc gctctacgcg cttacccgct ttatccggga aaaccacatt
    27721 tcccacctga tgatacacac cggcaaaatt gccgccttat ccgtactttt gaaaaaactg
    27781 accggggtgc gcctgatatt tgtcaaacat aatgtcgtcg ccaacaaaac cgatttttat
    27841 caccgcctga tacagaaaaa caccgaccgc tttatttgcg tttcccgtct ggtttacgat
    27901 gtgcaaaccg ccgacaatcc ctttaaagaa aaataccgga ttgttcataa cggtatcgat
    27961 accggccggt tccccccgtc tcaagaaaaa cccgacagcc gtttttttac cgtcgcctac
    28021 gccggcagga tcagtccgga aaaaggattg gaaaacctga ttgaagcctg tgtgatactg
    28081 catcggaaat atcctcaaat caggctcaaa ttggcagggc acggacatcc ggattatatg
    28141 tgccgcctga agcggggcgt atctgcttcg ggagcagaac catttgtttc ttttgaaggg
    28201 tttaccgaaa aacttgcttc gttttaccgc caaagcgatg tcgtggtttt gcccagcctc
    28261 gtcccggagg cattcggttt gtcattatgc gaggcgatgt actgccgaac ggcggtgatt
    28321 tccaatactt tgggtgcgca aaaggaaatt atcgaacatc atcaatcggg gattctgctg
    28381 gacaggctta cgcctgaatc tttggcggac gaaatcgaac gcctcgtctt aaaccccgaa
    28441 gcaaaaaacg cactggcaac ggcaggacat caatgcgtcg ccgcccgttt caccatcaac
    28501 cataccgccg acaaattatt ggatgcaata taagccgctt tcagacggcc tatgccgtct
    28561 gaaagccttt gatgcaacaa accgccaaat tatattcgtt cattggaaaa aaacaccccg
    28621 aattcatcct tcaaaataag aaaatcccaa tatcccccga tatcgcgcag cctattggca
    28681 aagttttgca gcgtcttccc cggcttgtgc cgccgcgtca agtgctttgt tacaatgcac
    28741 taccttcaca tttcttaata aattttatga gtaaccatac ttcttggtcg tccaaaatcg
    28801 gtttcgtact tgctgcggca ggttcggcca tcggtttggg cgcgatttgg aaatttcctt
    28861 atacggcggg caccaacggc ggcgcggtgt ttttcctgct gtttttgata tttaccgtct
    28921 tggtcgccct gcccgtccag cttgccgaat tttatatcgg gcgcacgggc ggtaaaaatg
    28981 ccgtcgattc cttcagggtt ctgcgtccgg gtacgcaatg gctttgggtc gggcgcatgg
    29041 gcgttgccgc ctgctttatt ttgctgtcgt tttacagcgt ggtcggcgga tgggtattga
    29101 attatgtcgt ccacagtttt acaggggcaa tccatgcggg agcggacttt gaagccctgt
    29161 tcggcacaac gatttccaat ccggcaggtt cgctgtccta tcaggcactg tttatgctga
    29221 ttacggtttg ggtggtcaaa ggcggcattt cagacggcat tgaaaaggca aaccgttatc
    29281 tgatgccggg gctgtttatc ctctttattg cgctggctgt ccgttcgctg acgctgccgg
    29341 atgcaatgga gggcgtgtct ttcctgctca aacccaattg gtcgtacttt aaagccgata
    29401 cgatgattac ggctttgggc caggcgtttt ttgccctgag catcggcgtt tccgccatga
    29461 ttacctatgc ttcgtatttg ggaaaagatc aggatatgtt ccgttccggc catacgatta
    29521 tgtggatgaa cctcttggtt tccctgcttg ccggcctggt gattttcccg gcggtattcg
    29581 ccttcggttt tgaaccgaac caggggccgg gattgatttt tatcgtattg cccgcagtgt
    29641 tcatgaaaat gccgttcggt acggttttat ttgcagtatt catgcttctg gtcgttttcg
    29701 ccacgctgac ttcggcattt tcgatgttgg aaacggtcat tgcctcaacc atccgccaag
    29761 acgagcgcaa acgcaaaaaa catacttggc ttatcggcac ggctattttc attgtcggca
    29821 tcccgtccgc gctgtctttc ggcgcatggg gtgagtttaa ggttttcggc aaaaccattt
    29881 ttgatttgtg ggactatgtt atttccgccg tcattatgcc gattggtgct ttgagtgttt
    29941 ccatctttac cgcctggatt caggacaagc agtctgtgtt aaaagatgcc ggcgcgggca
    30001 gcaccgtacc acgggcagtg ctgctgctgt ggctgaatac cttgcgctac cttgccccga
    30061 ttgccattat tattgttttc gtcaattctt taggcatcct ttaaacatac cgcccgacgg
    30121 caaaatgccg tctgaaagcc tttcagacgg cattttgctt cagattcaac ctatttcgtt
    30181 caaagtatag tggattaaat ttaaatcagg acaaggcgac gaagtcgcag acagtacaga
    30241 tagtacggca aggcgaggca acgccgtacc ggtttttgtt aatccactat attgtccggc
    30301 atggccagcc taactttaag ccgcctgctt tcggcaaccg accgtatcag cggcaaatcc
    30361 atcacggaaa acgccgtcga caaggcatcg gcaaccgccg catcatccgc cattacgctc
    30421 atgcttttat aacgcggcgt actcacgccg gttcgcggat cgaacaaatg ggtaagcctg
    30481 cccgctttgt ccatcaccgt cccatagccg cccgaagtgg caaacgcctt gtctttcatc
    30541 gtgatatttg ccaacacgcc ttcttcgtcg tccggattgc ggatgccgac attccacatc
    30601 cgcctgccgt ttgtatcaaa ccccctgatt tcgcccatat cgaccagcgc ggcaggcact
    30661 ccgtttgctt taagcagcgc aacaaccttg tccgtgatat agccttgcgc gatgccgttc
    30721 aaggacaaac ccataccctt ttcggcaaaa cggatttcac ggtcgtcaaa cgagactttg
    30781 ccgaagccga cccgtttcag ggtttccttc acgctattct ccggcggcgt ttccgcatcg
    30841 ggacggccgg caaaataatc ggcatacagt ttccacaacg cctgaaccgt cggatcgaac
    30901 gcgccttccg tcagcgcgtg aatatcgcgg ccaggctcaa cagttccaaa aaatccgccg
    30961 gaggcgaagt cagataaccg tcgcggttca gacggctgat caggctgtct tcacggtaaa
    31021 ggctgaacat tttttccaaa cgcgccactt ccgccaaaac cttattgacc aaatccgccg
    31081 cctgtctgtc gtccacgccg aacaggcgca gctccgcgcc ggaacccagc gcgacacctt
    31141 tccagaaaaa cacattttca tcgcgttttt tatccatgtt gcgtttttcg ccgccggcgg
    31201 caaaaggatt cggcaaaaaa gaaaccgccg caccggccac agcaacggcg gcagccgtca
    31261 gaaaacgtct gcgcccgaaa tgcctgccca taccgcctcc taaaccgaca ctaccgcctt
    31321 gatgtgcgga tgcggatcgt agccttccaa ctcgaaatct tcaaacttga aggcaaacaa
    31381 atccttgact tcaggattga ttttcatcac aggcaaggcg cgcggttcgc gttccaactg
    31441 caatgcggcc tgttcgaaat ggttacggta caaatgcgcg tcgccaaacg tatggacaaa
    31501 ctcgcccgcc tccagcccgc acacttgcgc catcatcatc gtcaacagcg cgtagctggc
    31561 aatattaaac ggcacaccaa ggaaaatatc tgcactacgc tggtaaagct ggcaggacag
    31621 tttgccgtcg gcaacgtaaa actgaaacag cgcgtggcag ggcggcaagg ccatctcatc
    31681 gaccaaagcc ggattccacg ccgacacaat caggcggcgc gagtcggggt ttttcttaat
    31741 ttgttccacc acattggcga tttggtcgat atgcctgccg tcgggcgcgg gccagctgcg
    31801 ccattggtag ccgtaaaccg gacccaagtc gccgttttcg tccgcccact cgtcccaaat
    31861 ggaaacattg ttatccttca ggtatttgat attggtatcg cctttgagga accaaagcag
    31921 ctcgtggatt atcgagcgca aatgcagctt tttggtcgtc agcagcggaa aacctttgcc
    31981 caagtcaaaa cgcatctgat aaccgaatac ggagcgcgta cccgtaccgg tgcggtctga
    32041 tttgtccgta ccgttgtcga ggacgtggcg catcaagtcc aaataggcct tcatcatcat
    32101 ctttcatcaa attaaacggc acatattgta acatttccgg ccaacacccg agtccggaca
    32161 aacgcgcagg ccgccccgcc ggacgctcaa aacgcggatg aaggcaggca ggattgttgg
    32221 caatttcagc cctttttcac agcaaaagca ggcgtgaaaa caaaattatc ttgattggaa
    32281 tcaaaaaatc tagtttaact acttagaata aaattccaat
//
LOCUS       DBJGGI010000016        32180 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.016, whole genome shotgun sequence.
ACCESSION   DBJGGI010000016 DBJGGI010000000
VERSION     DBJGGI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32180
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.016"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..230
                     /locus_tag="ACSIGJ_000752"
     CDS             <1..230
                     /locus_tag="ACSIGJ_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HHQ1329683.1"
                     /translation="GGFTRCRINRSKEFADRRNHINGIGNFWNQAKRALRKYNGIDRK
                     PFPPLLRECEFRFNFGTPSRQLKILRDRCGI"
     gene            complement(258..1328)
                     /gene="leuB"
                     /locus_tag="ACSIGJ_000753"
     CDS             complement(258..1328)
                     /gene="leuB"
                     /locus_tag="ACSIGJ_000753"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HHQ1329684.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKFIAQGLDADYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEHEGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTGD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            complement(1362..2095)
                     /locus_tag="ACSIGJ_000754"
                     /pseudo
     CDS             complement(1362..2095)
                     /locus_tag="ACSIGJ_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
     gene            complement(2111..3382)
                     /locus_tag="ACSIGJ_000755"
     CDS             complement(2111..3382)
                     /locus_tag="ACSIGJ_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HHQ1329685.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDSTQKIGRTLTVILETLEALGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETSPSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGAVTEEEKQLLNILLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            complement(3549..4190)
                     /gene="leuD"
                     /locus_tag="ACSIGJ_000756"
     CDS             complement(3549..4190)
                     /gene="leuD"
                     /locus_tag="ACSIGJ_000756"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HHQ1329686.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNGKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAIIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYRLSIDLAEQTLT
                     TPGGETFTFDITEHRKHCLLNGLDEIGLTLQHADKIKAFEEKRRQSQPWLFNG"
     gene            complement(4252..4506)
                     /locus_tag="ACSIGJ_000757"
     CDS             complement(4252..4506)
                     /locus_tag="ACSIGJ_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329687.1"
                     /translation="MNKLFVTALSALALSACAGTWQGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGKKIENATE"
     gene            complement(4601..6010)
                     /gene="leuC"
                     /locus_tag="ACSIGJ_000758"
     CDS             complement(4601..6010)
                     /gene="leuC"
                     /locus_tag="ACSIGJ_000758"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HHQ1329688.1"
                     /translation="MTAQTLYDKLWNSHVVREEGDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDQNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKGKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLNIGGKVPNPAEETDPVKRSGIERALEYMGLKAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKGHKKAGNVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPRQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGHFTDIRTMA"
     gene            6262..7611
                     /gene="gshA"
                     /locus_tag="ACSIGJ_000759"
     CDS             6262..7611
                     /gene="gshA"
                     /locus_tag="ACSIGJ_000759"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HHQ1329689.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWNAHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETEKSVLIIPE
                     NHTRNTFYLQNVYALGEIFRSAGCEVRLGSLNPEVTEPAEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGIGQTVLPPLHGGWTTRRKTNHFGAYNQV
                     AAEFAKLIDIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGIAD
                     KPFVIVKADAGTYGMGVMSVKSSDEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGVYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            complement(7585..7707)
                     /locus_tag="ACSIGJ_000760"
     CDS             complement(7585..7707)
                     /locus_tag="ACSIGJ_000760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329690.1"
                     /translation="MPSETDVPTRQISEMPSEPDFRFRRHLYFKTVLRRFFQLD"
     gene            7734..8411
                     /gene="radC"
                     /locus_tag="ACSIGJ_000761"
     CDS             7734..8411
                     /gene="radC"
                     /locus_tag="ACSIGJ_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HHQ1329691.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILEEELQEEIT
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSQTVRSF
                     RQLGLMP"
     gene            8484..9209
                     /locus_tag="ACSIGJ_000762"
     CDS             8484..9209
                     /locus_tag="ACSIGJ_000762"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HHQ1329692.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENMRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVAYWDFNWRKGGGGARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPV
                     KLGVLYYGDESTQYEPAPVRVDK"
     gene            9361..10641
                     /locus_tag="ACSIGJ_000763"
     CDS             9361..10641
                     /locus_tag="ACSIGJ_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HHQ1329693.1"
                     /translation="MKSLFIWLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRTQIGLHALAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDALMSAVYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGSFERACAKGRRQPEAGRKYYRNACHNGAAVYADEAMP
                     VTELLYTAYPVGGGALPYFYGERPDPVPEYEITGNPASIDFSEAAGKIAMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAHQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRRQFGNSLSILRHE
                     AGGIVFSVSGMAGSRIRLTPEDSPERGVTLYLQD"
     gene            10647..11825
                     /gene="hflX"
                     /locus_tag="ACSIGJ_000764"
     CDS             10647..11825
                     /gene="hflX"
                     /locus_tag="ACSIGJ_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HHQ1329694.1"
                     /translation="MSGRTGRNSATQAQPERVMLVGVMLDKDDTGSNAARLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKILQCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLTAHRINALKKQLANLKKQRALRRKSRESGRIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPACSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAHDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAEYCAAAPNTDETEMP
                     "
     gene            11822..14293
                     /locus_tag="ACSIGJ_000765"
     CDS             11822..14293
                     /locus_tag="ACSIGJ_000765"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HHQ1329695.1"
                     /translation="MKKTCFHCGLDVPENLHLTVRYEGEDRETCCVGCQAVAQSIIDS
                     GLGSYYKRRTADAKKTELPPQEILDQIRLYDLPEVQSDFVETHNGTHEAVLMLSGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQTGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRAGMDTPIAAAIIMTFIAGIYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHRMPGYPAVQDVRESAVVKLQ
                     AGDIVMVKPGETIPVDGTVLEGNSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLSQTTD
                     IIFDKTGTLTQGNPAVRRIELLGSMTEAQVLAVAQSLEQQSEHPLARAILNCRISGGS
                     VPEIQVGQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLQDPLKDSAAEAVRQLAGKNLTLHILSGDREEAVAETARALGIAHYRA
                     QAMPEDKLEYVEALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMPSEKILSEQ"
     gene            complement(14952..15728)
                     /locus_tag="ACSIGJ_000766"
     CDS             complement(14952..15728)
                     /locus_tag="ACSIGJ_000766"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HHQ1329696.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCIRDESLRFENGQFVMVGLMA
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDEVLISKKPTGTLVAC
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHRGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(15916..16710)
                     /locus_tag="ACSIGJ_000767"
     CDS             complement(15916..16710)
                     /locus_tag="ACSIGJ_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329697.1"
                     /translation="MTLPMQETRFSILLDELAAKQEATIAPYLLADGTKVWVRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSAGIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKRQFLSQAFARNMMWDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAGTVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            complement(16757..18166)
                     /gene="thrC"
                     /locus_tag="ACSIGJ_000768"
     CDS             complement(16757..18166)
                     /gene="thrC"
                     /locus_tag="ACSIGJ_000768"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HHQ1329698.1"
                     /translation="MRYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMCLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPVR
                     CLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIRQVYEQDKELIDPH
                     TANGVKVAREVREAGETVVCLETALAAKFDATIREAVGDVAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            complement(18239..18607)
                     /locus_tag="ACSIGJ_000769"
     CDS             complement(18239..18607)
                     /locus_tag="ACSIGJ_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329699.1"
                     /translation="MNKTLSILPAAILLGGCAAGGNTFGSLDGGTGMGGSIVKMTVES
                     QCRAELDRRSEWRLTALAMSAEKQAEWENKICGCATEEAPNQLTGNDVMQMLNQSTRN
                     QALAALTVKTVSACFKRLYR"
     gene            complement(18858..20322)
                     /locus_tag="ACSIGJ_000770"
                     /pseudo
     CDS             complement(18858..20322)
                     /locus_tag="ACSIGJ_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
     gene            20577..21493
                     /locus_tag="ACSIGJ_000771"
                     /pseudo
     CDS             20577..21493
                     /locus_tag="ACSIGJ_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(21496..21708)
                     /locus_tag="ACSIGJ_000772"
                     /pseudo
     CDS             complement(21496..21708)
                     /locus_tag="ACSIGJ_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(22021..23625)
                     /locus_tag="ACSIGJ_000773"
     CDS             complement(22021..23625)
                     /locus_tag="ACSIGJ_000773"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HHQ1329700.1"
                     /translation="MNYKDDTRITGATVSDKGLVAIKPLNNTNIVADTIHYKGDVLAV
                     NKGKVELDFTPNIRLVGRLDNFSGLTDSKHKNLFENYVANLDSKSAGEINFNLAKDAL
                     WTMTGQSWLDKLEGQGTIDFNNDAKTSGRALHIGELAGANKFLMHLNKDGIHSDMLYV
                     KKGTSTPQEVVVKNLSEVLDSMNYGERLRFATVTNSKNEFVNGKKYIDDTHLMEDALT
                     VEYSAHNGDKNNKDDYNKSFNGSEMTAEKAGDDYVNKTYTDNRQNVYLVKQATGNPSR
                     NVKNINDMFDSTAHYAFTLDTYAKREGERAFSTLDKKEGDWIRLTHTRVIQSNAFRFH
                     NNDFEIGYDRFSLNEQEKKRKWGISLDYGHGRTSLWNTFGKDKIRKYELALYNTTQYI
                     DKEGDETGYIDNVLKIGKLRNRVIARNHMGQLWGKGKYSNTLFSISTEYGRRKFLDDD
                     KLWRITPQVQLQYSYLRGTGYRIDNGINVNLSHANSLIGRLGLDVVRKFDGGKKLFYI
                     KGNIFHEFLGSRSFKAFEGKSHYAQK"
     gene            complement(23743..24270)
                     /locus_tag="ACSIGJ_000774"
     CDS             complement(23743..24270)
                     /locus_tag="ACSIGJ_000774"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329701.1"
                     /translation="MNQEGITAHGNATITLKAKENNKITVENAAYSSDGISTLINRTG
                     ARPGTRDDGNKIILEAGGDNIVTMKSGDADADYVNNSKVLTETPYYKSKRGSNGIFAY
                     GDKSLVKLIGENNIVKSEISEKSKALNGGFRHIGIYSWQNAKVELSAKSDNIVQGGIW
                     GLYSNNSSISLKGKK"
     gene            complement(25070..25377)
                     /locus_tag="ACSIGJ_000775"
                     /pseudo
     CDS             complement(25070..25377)
                     /locus_tag="ACSIGJ_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714068.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25574..26884
                     /locus_tag="ACSIGJ_000776"
     CDS             25574..26884
                     /locus_tag="ACSIGJ_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HHQ1329702.1"
                     /translation="MTDLFVREPDAPLAERLRPHTLDDVIGQQHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIRGAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     SLSSDDLKKLIAKVLALPEYRDFTIEADVQELLVNTADGDARRLLNLLEQLLRAADTR
                     RLKILTTEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRRFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEALKAK"
     gene            27004..30009
                     /locus_tag="ACSIGJ_000777"
     CDS             27004..30009
                     /locus_tag="ACSIGJ_000777"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HHQ1329703.1"
                     /translation="MSQTHNENSRVKIPAVLHLMRLGYDYLSLKNANWDRQTNIFPEI
                     FVDSLCRINPDLPPDDARRLLADIRLELDNEDLGQKFYERLTNQSGGKKLIDFQNFDN
                     NSFHVVTELPCINGDEAFRPDIALLVNGMPLVFIEVKKPNNKGGIGEERERMGKRAKN
                     PKFRRFINITQFMIFSNNMEYDDGATEPAQGAFYASSACGKPVFNYFREEHKLNLTEL
                     LDTLSDDLENNVLQDNNLPVIKHSPEFISNKSPDTPTNRILTSLLCRERLAFLLQHGL
                     TYVKASQGSVQKHIMRYPQLFATLAIEKHLSNGGKKGVIWHTQGSGKTAPAYYNTRYL
                     THYYAKQGIVPKFYFIVDRLDLLKQAQREFTARDLIVHTIDSREAFAADIKSAQTLHN
                     HAGKPEITVVNIQKFQDDPDVVARNDYDLAIQRVYFLDEVHRSYNPKGSFLANLNQSD
                     VNAVKIGLTGTPLIGVTAGNVNTRELFGDYIHKYYYNASIADGYTLRLIREEIGSRYK
                     AQLQEALAQLEIEKGSFDRKEIYAHPHFVHPMLDYILDDFAKFRKTNQDESLGAMVVC
                     DSAEQARQLFEHFQTASDHNFTAALILHDVGTKEERDQWVKDFKAGKTDILFVYNMLL
                     TGFDAPRLKKLYLGRLIKAHNLLQTLTRVNRTYKSYRYGYVVDFADIEREFDKTNRAY
                     WDELSNELGDEIGSYSQLFKTAEEIEQEIADIKNALFDFDTENAEEFCSQISQIEDKK
                     QLLALKKALQTAKELYNILRLQGSHEFLAHLDFDKLNLLYRETAARLDTLNLAEKLQQ
                     GDTAHLLNEALEDVYFQFVKIGEAELKLADDLKDIMRKVREGLAGNFDQDDLEYISLR
                     EELERIFKKKNLAEVGQEEMQANIATLQTVYTKIKEPNRQNDLLRHKYGGDAKYARIH
                     KRLMENAALYGDKLKVFNALNGVKTDADQRVLDMEQILDNQNYFEKQMQGIVLKRFRT
                     EQQFPVQPADIQTINRLLVREYLKESGRI"
     gene            30058..30288
                     /locus_tag="ACSIGJ_000778"
                     /pseudo
     CDS             30058..30288
                     /locus_tag="ACSIGJ_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004143539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            30338..31540
                     /locus_tag="ACSIGJ_000779"
     CDS             30338..31540
                     /locus_tag="ACSIGJ_000779"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HHQ1329704.1"
                     /translation="MNHFKKQQIQNIADFNPREQLAKGALAKSVPMAMLKEFQRQITG
                     YEIKAFNGGAKFRNGDTLLAKITPCLENGKTAFVDILDDGEVAFGSTEFIVLRAKNET
                     NPEFLYYFAISPDFRKRAIECMEGTSGRQRVNENALKTLELPIPEPQIQQSIAAVLSA
                     LDKKIALNKQINTRLEEMAKTLYDYWFVQFDFPDANGKPYKSSGGDMVFDETLKREIP
                     KGWGSIELQSCLAKIPNTTKILNKDIKDFGKYPVVDQSQDFICGFTNDEKSILNPQDA
                     HIIFGDHTRIVKLVNFQYARGADGTQVILSNNERMPNYLFYQIINQIDLSSYGYARHF
                     KFLKEFKIILPSKDISQKYNEIANTFFVKVRNNLKQNHHLTQLRDFLLPMLMNGQVSV
                     RCSGARDG"
     gene            31533..32093
                     /locus_tag="ACSIGJ_000780"
     CDS             31533..32093
                     /locus_tag="ACSIGJ_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329705.1"
                     /translation="MDKLFKILLNISSTSLLLSIYFIKSQYYILDLTWFYRVISNHWI
                     EPISKLSLLFYFLIPFIATATVLWLSKYLGKDEFKQGEVKELEYVNDNFLPSYLGYFF
                     VALSIPDNNLFLLFVMYGIIFLLVSCSKSFYFNPVFFLFGYRFYQAKTESGLLLVLIS
                     KQEFRTPQSVSSIAVYRINNFTFLEK"
BASE COUNT         8031 a         8225 c         7882 g         8042 t
ORIGIN      
        1 cgggcggttt tacccgttgc cgcatcaacc gttccaagga atttgcagac cgtcggaacc
       61 acattaacgg cattgggaac ttttggaatc aggcaaaacg cgccttgcga aaatacaacg
      121 gaatcgatcg taaacctttc ccgccgttgt tgagggaatg cgaatttcga tttaacttcg
      181 gcacaccgtc ccggcagcta aaaatcctgc gggatcggtg tggaatttag ggataatcta
      241 gtacagcccc ttgtttttta caaggcggcg agtaccgcgt cgcccatttc ggagcaggaa
      301 acgagttttg tgccttcttc gtaaatatcg ccggtacgca agccttgttg cagcactttt
      361 tggacggcgt tttcgacttg ttgcgcgcgc gcttcgtcgt tcaggctgta acgcagcaac
      421 atggcaagcg agaggatggt ggccagcggg ttggctttgt tttgtccggc gatgtcgggg
      481 gcggagccgt gagacggttc gtacaggcct ttgccgtttt cgtccaaaga agcggaaggc
      541 agcataccga tggagccggt cagcatggag gcttcgtcgg agaggatgtc gccgaagatg
      601 ttgccggtgg caatcacgtc aaactgtttg ggcgcgcgca cgagttgcat ggcggcattg
      661 tccacataca tatgggaaag ctcgacatcg ggatattgtt tgccgatttc ttcaaagatt
      721 tcgcgccaca gttcggtggt ttccaaaacg ttggctttgc ctacggagca gacttttttg
      781 ctgcgttttt gggcggattg gaaggccacg tgggcgatgc ggcggatttc gctttcgctg
      841 tatttcatgg tgttgtagcc ttcgtgttcg ccgttttcca aaacacggat gccgcgcggt
      901 tcgccgaaat aaatatcgcc ggtaagttcg cgcacaatca aaatatccaa accggcaacg
      961 atttcaggct tgagcgtgga ggcgttggct aattcgggat ataaaacggc aggacgcaaa
     1021 ttggcaaaca ggttcaaatc cttacgaatt gctaacaagc cgcgctcggg gcgcaacgga
     1081 cggtcgagat tgtcgtattg gggcgaaccg actgcaccaa gcaggacggc atcggctttg
     1141 cggcagaggt tttgcgtaaa ttctggataa ggatggccgt attcgtcgta ggcttcgccg
     1201 cccaaaggcg cgtattcgta gtcggcatcc aagccttggg cgataaattt gtcgagtacg
     1261 cggacggttt cggcgacgat ttcgggaccg atgccgtcgc ctcggaggat ggcgatatgt
     1321 ttggtcattt caagtttcct tatgggttga tggttgaagg gttatttctt tttgtatttg
     1381 tgtgcaattt cgtgccaacg gggtatggaa atcgatcggt tgtagtgttt tttataagct
     1441 tcctcaaatt tctttttcca taaggatgcg ttatgccgtg ttgccgggtt ttgataaacg
     1501 gtttcttcaa ttgcggaaac aaaatcttcc aagcattcaa acataggcat attcttttta
     1561 tttatgctgt accaaacacc cgggcggatt ttatgcacca cgcctttttt gacttgaaga
     1621 ttaattgccc acccatccat gcttctggta atttgccata cttttttaag ccataaatcc
     1681 ttgatggtta cattgccgtt gtcgtccata tcgtaaccga atattaaata atctacatcc
     1741 agcatatagg gcttatgaat gatttcatca gaatacattt taaaatctgc aatatcaaaa
     1801 cccgggcagg catttcggtt gaacgccttt acttccaaca attctctgct gcggcctttt
     1861 ttatttaaaa aaaaaaatcg ggaggcattt gggtattggt tgaaacatca aattcaattt
     1921 ccctttttct caaccatccg ccgagccatt cctgaatgat gttgccgaca acatcttttt
     1981 gtttgacgat aatatccaca tcgcctaaga aaaatctaat ttgaccattg gccgataaga
     2041 ttttttcttc atccagcaac ttatcaaata tttgttgtgc agtaagtttt atcattttat
     2101 ccctgtcggt ttataaagta tgcagaagtc tttcagatac cgccttaatc acagggacgg
     2161 caacggtatt acccaataaa tcgcatctgt cttttttggg aatatcaaac gaataatcgt
     2221 ccggatagcc gaataagcgt aacccttctt ttccggtaag tgtacgcaaa ccgccgttgt
     2281 caacgacgaa aaggtgctcc atatccattg caaccaaggt tggcgcaaca tcgtttggat
     2341 ctaatatttt attgatttca aaagattttt tacctgaaac aatattgtag cctttgggca
     2401 gggattcatc tttgattctt tgcccgccaa ttttttgttt cggatgctct aaaaccaaat
     2461 agcctttgtc tgtcaggctg tccaaaatat tttggagatc ggggtgttta tagaaagttg
     2521 aaatttgcgc ttttgtcaaa ggcatcccat ccatccaatc tatgccgatt tctgaagccc
     2581 attttttttt cctccgttct tttagaagga tatttaacaa ttgcttctct tcttcggtta
     2641 ctgcgccttt taattcaata tcccaactgt gaatattgtt tttccctccc cgtttgtctt
     2701 ttactgattt tccgtacagt tcggacgggg gaaatttttt tagcaatttt ttgatgaaag
     2761 ggctgctttc agtaggcagt cccgattcca aaatattttt taatttcgga cttgggcttg
     2821 tttcgaaaga taagtcgggt ttggatttca aactgcctgt cagataaatg cgcttcctgt
     2881 tttggggaat gccgaaatct tttgcattta aaactttcca agaaacatag tagcccaatg
     2941 cttccaaggt ttccaaaata acggtcaggg tgcgccctat tttttgtgtc gaatcttttc
     3001 tatcgtgtgt caccaatcct tccacatttt ccaaaataaa accttttggt ttttttgcct
     3061 ttaaaatcct tgccacatca aagaaaagcg ttccccgtgt atcttcaaag cctaatcttt
     3121 ttccggcgaa agaaaaagcc tgacaaggga agcctgccaa caggatgtca aaatcgggaa
     3181 tatcccctgt ttcaattttc gttatatctc catacggcac ttcatcaggg tagttttgct
     3241 tcaatacttc caaagctgcc ggtttgattt ctgaagtaaa aacacattcg caagcaaccg
     3301 actgtttccg acaggcttgt tcgaatcctt tcctgatgcc actcattccg gaaaataagt
     3361 caataaattt aatttgttgc atattaaaaa tctaaaaatt tatttgaaat ggagagttgc
     3421 attaatttag agtgttgcta agcccgctta aaagatgaaa acaatttatc gcccctctgt
     3481 ttacattagc cgcagcaatt atatgttatc aggaatgccg tctgaacagc cttcagacgg
     3541 cataggtttt aaccgttaaa cagccaaggc tggctttggc ggcgtttttc ttcaaaggct
     3601 ttaattttgt cggcgtgttg cagggtcagt ccgatttcgt ccaagccgtt taagaggcag
     3661 tgtttgcggt gttcggtaat gtcgaatgtg aatgtttcgc cgcccggggt ggtcagggtt
     3721 tgctcggcaa ggtcgatgga gagccgatag ccttcgttgg cttcgacttc tttgaaaagc
     3781 cggtcgactt gttcttcggt caacacgatg ggcaaaaggc cgtttttgta gcagttgtta
     3841 aagaagatgt cggcgaagct gggggcgata atggcgcgga agccgtagtc gtccaatgcc
     3901 caaggggcat gttcgcgtga agagccgcaa ccgaagtttt tacgcgtcaa caggatttgc
     3961 gcgccttggt aacgcggctg gttcagggaa aaatcggggt tcaacgggcg tttgccgttg
     4021 tccatgcccg gttcgccgtg gtcgaggtaa cgccattcgt caaaggcgtt ggggccgaag
     4081 ccgctgcgtt tgatggattt cagaaattgt ttggggatga tggcatcggt atcgacgttg
     4141 ctgcggtcga gcggggcgac gatggcggtg attttggtaa aggctttcat gggtttgcgt
     4201 cttgtgctga cgatgccgtc tgaaacggtt tcagacggca tcgcaaatcg gttattcggt
     4261 ggcgttttcg atttttttgc cgagatggga aatgccgcgt ccgacggcat tgccgccttt
     4321 tttgacggct tctttggttt tgtcccaacc tttttcgacg gcgttgcctg tttgttcggc
     4381 ggcgcgttcg gcggcggcct gtgttttgtc aaggttgcgg gcggtgtctt gtttcgcgcc
     4441 ctgccaagtg ccggcgcagg cggacaaggc gagggcggac agggcggtaa cgaaaagttt
     4501 gttcatggtt aaactccttg gtttgaatat taggtgtttc tgccttacgg gacatatttc
     4561 agacggcctc gtcaaattct taaagactgc ctgaaaatac ttacgccatc gtgcggatgt
     4621 cggtaaagtg tccggtaacg gcggcggcgg cggccatagc ggggctgacg aggtgggtgc
     4681 gcccgccgtt gccttggcgg ccttcaaaat tgcggttgga cgtggaggcg cagcgttgcc
     4741 tcggcgcgag gcggtcggca ttcatcgcaa ggcacatcga acagcccggt tcgcgccatt
     4801 caaaaccggc ttcgatgaaa attttgtcca agccttcttt ttcggcttgt tctttaacca
     4861 aaccggagcc ggggacgatt aacacgcgct gtacgttgcc ggctttttta tggcctttgg
     4921 cgatagcagc ggcttcgcgc aagtcttcga tgcggctgtt ggtgcaagag ccgataaaga
     4981 cgatatcgac agggatttcg ttcagcggcg taccggcctt caaacccata tattcgagtg
     5041 cgcgctctat gccgctgcgt ttgaccggat cggtttcttc ggcgggattc ggcactttgc
     5101 cgccgatgtt taaaaccatt tcgggcgacg tgccccaagt aacttgcggt tcgatgtctt
     5161 cggcgttgaa acggtattct ttatcaaaaa ccgcgccttc gtcagacacc agcgtgcgcc
     5221 agtactcgac ggctttgtcc caagcttcgc cttcgggtgc gaagggcttg cctttgacat
     5281 agtcgatggt ggtttgatcg acggcaacca taccggaacg cgcgcctgcc tcaatcgcca
     5341 tattgcacag ggtcatcctg ccttccatcg aaaggctgcg gatggcttcg ccgccgaact
     5401 cgacggcgta acccgtgccg cccgccgtgc cgatttgccc gatgatgtag agcgccacgt
     5461 ctttggcggt aacgcccgct tttaatttgc cgtcaacggc aatcagcata gatttggatt
     5521 ttttcgcggt aatgcactgg gtcgccatcg tgtgttcgac ttcggaagtg ccgatgccgt
     5581 gtgccagtgc gccgaatgcg ccgtgggtgg aagtgtgcga atcgccgcag acgacggtca
     5641 tcccgggcag ggtcgcgcct tgttcgggcc ccataacgtg tacgatgccc tggcctttgt
     5701 ccataaacgg gaagtaggcg agtgcgccaa actctttgat gttttgatcc aacgtatcga
     5761 cttgcagctt ggaaatcggg tcttggatgc ctttgtccca atcgccggtc ggggtgttgt
     5821 ggtcggcggt ggagaccacg ctgtcgatgc gccacagctt gcgccccgcc attttcagac
     5881 cttcaaacgc ctgcgggctg gttacttcgt gaaccagatg gcggtcgatg taaagcagga
     5941 cggtgccgtc tccttcttcg cggacgacgt ggctgttcca gagtttgtcg tagagggttt
     6001 gtgccgtcat gatgttgttc ttttggataa atggtgatgc ggattgggcg gattttagac
     6061 gtattcttta tactgcgcaa cagattttgt ctaatttttg agtcggtgtt attttgtaaa
     6121 caattttaac gaaaaaatta gacatattgt tcatttcgtt atatctaaag aatgattcga
     6181 tacgaaagaa tacttgtcgt cattctttca aaagcattat tatctgtacc ttgtcaaaaa
     6241 acacacagag gcagacgaaa gatgaaatta ccggttatgt cgcccgaaca ttcggcgcaa
     6301 cttcaggcgt ttgaggcaaa aatcctgtcc aatcacgcca aaatcgaggc gtggttccgc
     6361 acgcagtgga acgcgcaccg cccgccgttt tacggttcgg tcgatatacg caacgccggt
     6421 tacaaaattt cgtctatcga tatgaatttg tttccgggcg gcttcaataa tctgaatccg
     6481 aatttcatcc cgctggcggc ggttgccgcg caagatgcgg tgcaacgcgc ctgcgaaacg
     6541 gagaaatccg tattgattat tcccgaaaac cacacgcgca atacgtttta cctgcaaaac
     6601 gtttacgccc tcggcgagat tttccgttcg gcagggtgtg aagtgcgctt gggcagcctg
     6661 aatccggaag taaccgaacc ggccgaattt gaaactgcat tgggcgacaa aatcctgctt
     6721 gagcctttgc tgcgaacccg cgaccgcgtc caccttgccg acggcttttc gccttgcgtg
     6781 gttttgttga acaacgattt gtccgccggc attcccgaca ttctcaaagg catcggccaa
     6841 accgttttgc cgccgctgca cggcggttgg acgacgcgcc gcaaaacaaa tcatttcggc
     6901 gcgtacaacc aagttgccgc cgaatttgcc aagctgattg acatcgatga atggcagatc
     6961 aacccgtatt ttgaaaaaat cggcggtttg gacttccaag ggcgcgaagg tgaagatgcg
     7021 ctggcggaag cggtagaacg cgtgctggcg aaaattcaag ccaaatacga cgaatcgggc
     7081 attgccgaca aacctttcgt catcgtcaaa gccgatgccg gcacttacgg catgggtgtg
     7141 atgagcgtca aatcctccga cgaagtgcgc ggcttgaacc gcaaaaaccg caacaaaatg
     7201 gcgaaggtga aagagggctt ggaagtcagc gaagtgattg tccaagaagg ggtttatacc
     7261 tatgaaacct tgaacggcgc ggtgtgcgaa cccgtcgtct atatgatgga ccgtttcgtc
     7321 atcggcggct ttttccgcgt acacgaaggg cgcggtgcgg acgaaaacct caacgccggc
     7381 ggtatggtgt ttgtgccgct gtccaacagc attcctaccg gtaacggcga taattcgcaa
     7441 gaagctccag aagcctgcaa acgcgtattc gaacaatggg attcgctcgg tatgccgcgt
     7501 tctgaaaaag actgcgacgt ggacaacgaa cataaccgcc tctacgttta cggcgtaatg
     7561 gcacgtctgt cgcttttggc ggcttcaatc gagttggaag aaacggcgta agactgtttt
     7621 gaaatacaga tgccgtctga agcggaaatc cggttcagac ggcatttcgg atatttggcg
     7681 tgtgggaaca tctgtttcag acggcatctc agactattta aaaaagggaa aacatgagca
     7741 tcaagcaatg gccggaaggc gaaaggccca gggaaaagct gttggaacgc ggggcggcgg
     7801 ctttgagcga tgccgaactt ttggcaatcc ttttacgcgt cgggacgcgc gggatgagcg
     7861 cggtcgattt ggcgcgctac ctattgcagg agttcggcag cttggggagg ctgatgagcg
     7921 cggaggtcgg caaactgtcg gcatacaaag ggatggggac ggcaagtttc acacagttcg
     7981 ccgtggtcag ggaaatcggg cggcggatat tggaggaaga attgcaggaa gaaattacct
     8041 tatccgatcc cgataccgtt gccgattatt tacgctttca tttggggcag gaaaaagtcg
     8101 aagtcagcgt cgcgctgctg ctgaaccgcc aaaaccaact gattgcggtc agagagctgt
     8161 cgcgcggtac ggttgcggaa aacacgattt acatccgcga aatcgtcaaa ctggcattgg
     8221 acgaatatgc cgacagcctg attatcgcgc acaaccatcc gggcggctcg cccgaacctt
     8281 cgcaggaaga catcatgttc acaaggcggc tggcacaggc catgtcgctg gttgatgttt
     8341 cgctgctcga ccattttatc gttacctcgc aaaccgtccg ttcgttcagg cagctcgggc
     8401 tgatgccctg acaccctgtt ttacattcgg cggctctggt aaaatagccg ctttgaccgt
     8461 attcgacaga tattgttaag ttaatggaaa cacaaaacaa acctaccgtt accgacattg
     8521 accgccccat actcgtcccg cccggcggac ataaaaaagt cttgctgcat tcctgctgcg
     8581 ccccgtgcag cggcgaagtg atggaagcca tgcttgcctc cggcatcgac tacaccattt
     8641 atttttacaa tcccaatatc catccgcaca aagagtatat gctccgaaaa gaggaaaaca
     8701 tgcgctttgc ggaaaagttc ggcatccctt tcatcgataa agacgacgac tacgaaaacg
     8761 accgcaaaga atggtttgcc aaagccaaag gcatggagtt tgagccggaa cgcggcatcc
     8821 gctgcaccat gtgtttcgat atgcgttttg aaaaagcggc gcaatacgcg cacgaacacg
     8881 gttttcccgt ctttaccagt tcgctgggca tttcacgctg gaaaaatatg gcgcaaatca
     8941 acgactgcgg acaccgtgcc gccgcgcctt acgatgatgt ggcctattgg gacttcaact
     9001 ggcgcaaagg cggcggcggc gcgcgtatga ttgaaatcag caaacgcgaa aacttctacc
     9061 agcaggaata ctgcggctgc gcctattcct tgcgcgattc caacgcccac cgcaaatcgc
     9121 agggacgcat ccccgtcaaa ctcggcgtgc tgtattacgg cgacgaatcg acacaatacg
     9181 aacctgcacc cgtccgagtg gacaaataaa cacccgatgc cgtctgaagg ttcagacggc
     9241 atcgggttcg gcaccggcac gggaaaggtt tgccggtttg gcaatcggca agcggaaacc
     9301 gcattggcaa gtttgccgtt ttgataaaac accccgttgc cgcgtcggga gggcggcatt
     9361 atgaaatccc tttttatttg gctgcttcta ttgggctcgg cggcaggcgt tttctaccat
     9421 acccaaaacc aatccctgcc cgcgggcgaa cttgtctatc cgtccgcacc gcaaatcagg
     9481 gacggcggcg atgcgctgca ctacctcaac cgcatccgca cacaaatcgg tttgcacgcg
     9541 ctggcacacg cgccggtttt ggaaaattcc gcccgcaggc acgcacgcta tctcacgctc
     9601 aatcccgaag acggacacgg cgaacaccat cccgacaatc cgcactacac cgcacaaaag
     9661 ctgaccgaac gcacacgcct tgccgggtat ctctacaacg gcgtgcatga aaacatcagc
     9721 acggaagagg aagccgccga atcgtccgac agcgacatcc gcacgcagca acgccaagtg
     9781 gacgctttga tgagtgctgt ctatcaccgc ctttcgctgc ttgaccgcca taccgacgaa
     9841 gcaggtgcgg catttgtgcg cgaaaacggc aaaaccgtcc tcgtattcaa tcagggcaac
     9901 ggcagcttcg agcgcgcctg tgcaaaagga aggcggcagc cggaagcagg acggaaatat
     9961 taccgcaacg cttgccacaa cggtgcggcc gtttatgctg acgaagccat gcccgtaacg
    10021 gaattgcttt ataccgccta tccggttggc ggcggcgcgc tgccttattt ttacggggaa
    10081 cgtcccgacc ccgtgccgga atatgaaatc acaggcaatc ctgccagcat tgatttttcc
    10141 gaggcggcag gcaaaattgc gatgaaaagt ttcaagctgt atcagggtaa aaacgaaatc
    10201 cgccccgtca gggttttaac cgccggcaac gaccctaacg gcaggctgac cgcgcaccaa
    10261 ttcgcccttt tcccgctcaa acctttggaa tacggcacgc tttatacggc ggtattcgac
    10321 tatgtccgca acggacggcg cgcgcaggcg aaatggcagt ttagaacccg aaaacccgat
    10381 tacccttatt ttgaggtaaa cggcggcgag acacttgcgg ttagaaaagg cgaaaaatat
    10441 ttcatccact ggcgcggacg ctggtgtctg gaagcgtgta cccgttatac ctaccggcgg
    10501 cagttcggca acagcctgtc catactccgg cacgaagcgg gcggcattgt cttcagcgtc
    10561 agcggaatgg cgggaagccg catcaggctt actccggaag acagcccgga acgcggtgta
    10621 accctttatt tgcaggattg acagttatgt caggtagaac aggacggaac agtgccactc
    10681 aggcgcaacc ggaacgcgtc atgctggtgg gcgtaatgtt ggataaagat gatacgggca
    10741 gcaatgccgc ccgtctgaac ggttttcaga cggcattggc ggaagccgtc gagctggtca
    10801 aagcggcggg cggcgattcc gtacgcgtgg agactgccaa acgcgaccgc ccgcacactg
    10861 cgctgtttgt cggcacgggc aaggcggcgg agctgtcgga agcagttgcc gcagacggca
    10921 ttgatttggt cgtattcaac cacgaactta ctcccacgca ggaacgcaat ttggaaaaaa
    10981 tcctccaatg ccgcgtattg gacagagtgg ggctgattct ggcgattttc gcccgccgcg
    11041 cccgcacgca ggaaggcagg ctgcaagtcg agttggcgca attgagccat ttggcgggac
    11101 gcttgatacg cggttacgga catttgcaaa gccagcgcgg cggtatcggc atgaaagggc
    11161 cgggcgaaac caaactggaa accgaccgcc gattaaccgc ccatcggatc aacgccttga
    11221 aaaaacagct tgccaacctc aaaaaacagc gcgccctgcg ccgcaagtcc cgcgagtcgg
    11281 gcagaatcaa aacgtttgcg ctggtcggct ataccaatgt cggcaaatcc agcctgttca
    11341 accggctgac caagtcgggc atatatgcga aagaccagct tttcgccact ctcgacacga
    11401 cggcgcggcg gctgtacatc agtcccgcat gcagcattat cctgaccgat accgtcggat
    11461 tcgtcagcga tctgccgcac aaactgattt cggcattttc cgccacgctg gaagaaaccg
    11521 cgcaagccga tgtgctgctg cacgtcgtcg atgccgctgc cccgaacagc ggacagcaga
    11581 ttgaagacgt ggaaaacgta ctgcaagaaa tccatgccca cgatattccg tgcatcaagg
    11641 tgtacaacaa aaccgacctg ctgccgtctg aagaacaaaa cacgggcata tggcgcgacg
    11701 ctgcgggaaa aattgccgcc gtccgcattt ccgttgctga aaataccggt atagacgcac
    11761 tgcgcgaagc cattgccgag tattgtgccg ccgcaccaaa cacagacgaa accgaaatgc
    11821 catgaaaaaa acctgtttcc actgcgggct ggacgttccc gaaaacctgc atctgaccgt
    11881 ccgctacgaa ggcgaagacc gcgaaacctg ctgcgtcggc tgtcaggcag tcgcacaaag
    11941 catcatcgat tccggcttgg gcagttatta caaacgacgc accgccgacg cgaaaaaaac
    12001 cgaactgccg ccccaagaaa tcctcgacca aatccgcctg tacgacctgc ccgaagtcca
    12061 atccgacttt gtggaaaccc ataatggcac gcacgaagca gtgctgatgc tcagcggcat
    12121 tacctgcgcc gcctgcgtct ggctgatcga acagcagctt ttgcgcacag acggcatcgt
    12181 ccgcatcgac ctcaattaca gcacgcaccg ctgccgcgtc gtctgggacg acggcaaaat
    12241 ccgcctctcc gacattctgt tgaaaatccg gcagacaggc tacaccgccg caccctatga
    12301 cgcgcaaaaa atcgaagccg ccaaccaaaa agaacgcaaa caatacatcg tccgccttgc
    12361 cgttgccgga ctgggtatga tgcagacgat gatgttcgcg ctgccgacct acctttacgg
    12421 cggcgacatc gaacccgatt tcctgcagat cctccattgg ggcggcttcc tgatggtgct
    12481 gcccgtcgta ttttattgcg ccgtcccgtt ttatcaaggc gcgttgcgcg acctgaaaaa
    12541 ccgccgcgcc ggtatggaca cgccgattgc cgccgccatc atcatgacct tcatcgccgg
    12601 catttacagc ctcgccacca acgcagggca ggggatgtat ttcgaatcca tcgcgatgct
    12661 gctgtttttc ctgctgggcg gacgctttat ggaacacatt gcccgacgca aggcaggcga
    12721 tgccgccgaa aggctggtga aactgatacc cgccttctgc caccggatgc ccggctatcc
    12781 tgccgtacag gatgtccgcg aatccgccgt cgtcaaactc caagcaggcg atattgtgat
    12841 ggtcaaaccc ggcgaaacca tccccgttga cggcacggtg ctggaaggaa acagtgccgt
    12901 caacgaatct atgctgaccg gcgagagcct gcccgtcgcc aaaatgccgt ctgaaaaagt
    12961 aaccgcaggt acactcaaca cgcaaagccc tctgattata cgcaccgacc gcaccggcgg
    13021 cggcacgcgc ctgtcgcaca tcgtccgcct gctcgaccgc gccttggcgc aaaaaccgcg
    13081 tactgccgag ttggcggaac aatacgcctc atctttcata ttcggcgagc tcctgcttgc
    13141 cgtccccgtc ttcatcggct ggacgctgta cgccgatgcg cacaccgcat tgtggattac
    13201 cgtcgccctg ctggtcatta cctgcccctg cgccctgtcg cttgccacgc cgaccgcgct
    13261 ggcagcttct accggtacgc tggcgcgcga aggtatttta atcggcggaa agcaggcaat
    13321 cgaaaccctc tcccaaacca ccgacatcat tttcgacaaa accggcacgc tgacccaagg
    13381 caatcccgcc gtccgccgga ttgaactgct cggcagcatg accgaagccc aagtgctggc
    13441 agtggcgcaa agtttggaac agcagtccga acacccgctc gcgcgcgcca ttctcaactg
    13501 ccgcatttca ggcggcagcg tccccgaaat ccaagtcgga caacgcctca accgcatcgg
    13561 ggaaggcgtg ggtgcgcaac tgaccgtcaa cggcgaaaca caggtttggg cattggggag
    13621 ggcatcctat gttgccgaaa tttcaggtaa agaaccgcaa acagaaggcg gcggcagcgc
    13681 ggtttacctc ggcagtcaaa gcggtttcca agccgtgttc tacctgcaag acccgctcaa
    13741 agacagcgcg gcggaggcag tgcggcagtt ggcaggcaaa aacctgacgc tgcacatcct
    13801 cagtggcgac cgtgaagaag cagttgctga aaccgcccgt gccttgggta tcgcgcacta
    13861 ccgcgcccaa gcaatgccgg aagacaagct ggaatacgtc gaagccttgc aaaaagaagg
    13921 gaaaaaagtg ctgatgatag gcgacggcat caacgacgcg cccgttttgg cgcaggcaga
    13981 cgtatccgcc gccgcagcgg gcgggacgga tattgcgagg gacggcgcgg acattgtgtt
    14041 attgaacgaa gatttgcgta ccgtcgccca cctgctcgat caggcgcggc gcacccgcca
    14101 tattatccgg caaaacctga tatgggcggg cgcgtacaac atcatcgccg taccgcttgc
    14161 cgttttgggc tatgtccaac cgtggatagc cgcactgggt atgagtttca gttcgctggc
    14221 ggttttgggc aatgccttgc gccttcacaa acgggggaaa atgccgtctg aaaaaatact
    14281 gtccgaacaa tgacgggcgg cgttgcttta gacgtatagt cgattaaaac aaaaatgaga
    14341 cgatgaagga ttacaaactt aaagtatgca ttgttaccgc tcaaacacgt tggcgtttaa
    14401 aatttgagat cgaattgcta ggtatttgat tttattaaat aagagattat attaatgagg
    14461 aaaaaaatta aaaagtggat ttatgcgata aatggaataa tgatgtcaat atggattatt
    14521 tttttttcca aataccggtt ctgtgtatga cggtggcaga acaaccattt tcaatgaaaa
    14581 ccatcctttt cattttattt tctgcataac atttcttatt gggataattt ttattatata
    14641 tcaatcatat aatgataatt aatttttaac atccttactg tcctatcatg atgaaatgac
    14701 aataaggatg ttttcctgct ttggctacgg caaaacaccg tcgtcattcc cacgcaagcg
    14761 ggaatctata tgtttggttt ctcttttatt ttcaaacgat aatgaaacag gtaagtccgg
    14821 attcccacct gtgttggaat gacggaaatg tgcatttcta atttttaccc actataaaaa
    14881 ccccgaatcc tgattggcag gattcgggtt tttttatcgc tgatgccgtt cagacggcat
    14941 tttcaagcgg cttattgatc cacaaacgcg cgctcaatca ggtagtcgcc gcgcacgccg
    15001 gttttcgggg agactgtcag accgaaatcg tccaaaactt tgcaggtgtc tttcagcatc
    15061 gcagggctgc cgcacagcat ggcacggtcg tcttgggggt tgattttggg caggccgatg
    15121 tcttcaaaca gtttgccgct taccatcagg tcggtcaggc ggccgcggtg ttcgaattct
    15181 tcgcgggaaa caatcgggta gtaaatcagt ttttctttaa ccaagtcgcc gaggtattcg
    15241 tgttcgggca attctttggt aaagcggtcg tagtatgcca aatccttttt gtagcgcacg
    15301 ccgtgtacaa ggatgatttt ttcaaactgt tcgtaaattt cggggtcttt ggtgatgctc
    15361 aagaaagggg cgatgccggt accggtactc aacaggtaaa ggtgtttgcc cggattcaga
    15421 tcgcaggcaa ctagagttcc ggtcggtttt ttgctgatta acacttcgtc gccgactttg
    15481 aggtgttgca ggcggctggt cagcgggccg tcttggactt taatgctgaa aaattcgagg
    15541 tgttcttccc agttggcgga ggcgacgctg tatgcgcgca tcagcggctt gccgtccgcc
    15601 atcagcccga ccatgacgaa ctgtccgttt tcaaagcgca acgattcgtc gcggatgcag
    15661 gtaaaggtaa aatatgcgtc tgtccagtgg tgtacggaca atactttttg ggtattgaat
    15721 gctgccattt gggtttcctg tcagtaaaag aaatggatag tgcttgttcg ggacgtgcgg
    15781 cagagtggaa atgtctgccc gattcgggat aaagtcaaaa ttctaaacga aacggatggt
    15841 tgcgacaatg cctgatgcgc gttggttata tgccgtctga agggcttcgg acggcatcgt
    15901 ggggcgggcg gtggcttacg gcggcatatc ggcgagggaa attaaggaaa tcgaggctgc
    15961 cagcctgagc gcgtcccgtc cccagcgggg gtgttccagc ctgcgtatcg ggaggatcgg
    16021 tttgacggtg ccggcaatca gcttttgtat ttcggcaggc tgatccgaca atacgccgcc
    16081 ccatttttcc gcagccgctt ccagcagtcc gccgtttttc aatatcagcg cggtcgaatg
    16141 gatgtagcag agccaatcgc gggcttggca ttgcgctatg gtcaggacct cggaagggtc
    16201 gtcttcaaaa tccaaaaagc tgatgttttt tccgtcccac atcatattcc gcgcaaacgc
    16261 ctgactgagg aactgccgtt ttttatgcac gcgtgcaatg gcttccaaac cggcaagcca
    16321 agcgtccgcc tttccggctt cggcttcttg gcggatttgc gtatcgagcg ggatgccttc
    16381 caaattgccg aacataaggg catttttccg gacggcgagc aattcgggaa cggctatccc
    16441 tgccgaacgc aattcgtaca ggcgttttga ttcggttgca atggcaggct cgccgccgag
    16501 gctgggaacc ggcttcaaca cccccagttt caaatatcgg gcaaccatac cgagcagcgc
    16561 gtaacgccat cgcgcattgt gcctgcctgc tttgcgtacc cataccttgg tcccgtcggc
    16621 aagcaggtag ggggcgatgg ttgcctcctg ttttgccgcc aattcgtcca gcagtataga
    16681 aaaacgggtt tcctgcatag gtaaggtcat tttctttcaa tcttaagttc ggatggaatg
    16741 ccgcccatac ggaacatcag gcaagggttt gttcgatgat gccttttacc gcatccgcac
    16801 tgttcggcac ggtttgcacg cgctgcggca ggttttccaa accttccagc gcggcggggc
    16861 gcggaatggc gacatcgccg acggcttcgc gtatggtcgc atcgaatttc gccgccaacg
    16921 cggtttccaa acaaaccacc gtttcccccg cttcgcgcac ttcgcgggcg acttttacgc
    16981 cgttggcagt atgcgggtcg atgagttctt tgtcttgctc gtaaacctgt ctgatggtgg
    17041 cgaggcggtc ggcgtgggta gatttgccgg aggtaaagcc gtatttgccg ccgactttgt
    17101 ccaaggcaaa ccgcaggtca aagcctttgc ctgccgcgac ttccgcccac agcgtattga
    17161 tttcctgagg atcgcgatcc atcaggtcga acacgaaacg ctcgaagttg gacgctttgg
    17221 aaatgtccat agacgggctg gaggttacat aagtatgcgc gctgttgcgc gggcggtatg
    17281 cgccggtttt gaaaaactcg tccagcacat cgttttcatt ggtcgcgaca atcaggcagc
    17341 ggacaggcag gcccatttgt ttggcgatgt gtcccgcgca aacgttgccg aagttgccgc
    17401 tcggtacgca aaagctgacg gtttcgtcat tgcttgaagt ggcattgaaa tagcctgcaa
    17461 agtaataaac cacttgcgcg acgatgcgcc cccagttgat cgaattgacc gtaccgatat
    17521 ggtatttttc cttgaacgcg gcatcgttct gcactgcctt cacaatgtcc tggcagtcgt
    17581 caaacatccc cttcacggcg atattgtgga tattcccgtc ttgcaggctg tacatctgcg
    17641 cgcgttggaa cgcgctcatt ttaccgtcgg gcgacaacat aaatacgttc acgccttttt
    17701 tgccgcgcaa ggcatattcc gcagccgaac ccgtatcgcc gctggttgcg cccaagatat
    17761 tgagtttttt gccttctttg tttaaaacat attcaaacgc attgcccaaa aactgcatcg
    17821 ccatatcttt gaacgccagc gtcgggccgt tggacaaggc ttggattttg atgccgtctg
    17881 aaagcgtgcg gacgggggtg atttccttag taccgaacgc cgcttccgtg taagtacggt
    17941 tcagaatgtc gcgcaaatcg tcctccggaa tatcggtaac gaacaggcac atgatttcaa
    18001 acgccaattc gggataagcc aaaccgcgcc atttgtccaa ggtttcgcgc ccgatttgcg
    18061 gataatgttc cggcagcatc agtccgccgt cgggtgcaag ccccatcaat aaaacttcgc
    18121 tgaacggttt gtgtgcggtt tcgccgcgcg tgctgatgta tctcatgatt tttctcgttt
    18181 gtcgaaattg caggaaaacg gattcagacg gcatctgcct catgccgtct gaagaaggtt
    18241 agcggtacag gcgtttgaag caggcggaaa ccgttttgac ggtcagggcg gcaagtgcct
    18301 gattgcgcgt ggactggttc agcatctgca tcacatcgtt gccggtcagc tggttaggtg
    18361 cttcttcggt agcgcagccg caaatcttgt tttcccattc cgcctgtttt tcggcactca
    18421 tcgccagcgc ggtcaaacgc cattcgctgc gcctgtccaa ttccgcacgg cattggcttt
    18481 ctaccgtcat tttgacgatg ctgccaccca tacccgtgcc gccgtctaag ctgccgaatg
    18541 tgttgccgcc ggcggcgcac ccgccgagta agattgccgc cggcaaaata gacaaggttt
    18601 tattcatctc aattcctttt cggttgaaac cccgcctttt atggcgatag gatctgatta
    18661 gccgccccgt tcgggataac gcgaagggcg gcgttttatg cgccgttccg agtgttggag
    18721 caaaccgttt gaatatccgg ttgaagcccg gcaacattat acttcaatcg ggaaaataaa
    18781 aaatcccgcc gccgtcattt tgcctgtttg caaaaatgcc gtctgaaacc ggttcagacg
    18841 gcatttgcga tttcagccta gcccaaagat ttgaactgct ccaaaaacgg agggataccg
    18901 ggcagcatcc cgaccgcacc catcgccaca cacaagatta agaaacctac tgcgggtatc
    18961 aatactcgtc cgacaaaacc taattgcgcg ctgcgttctt tgcagccgat taagcccaaa
    19021 ttatccaaca gcatcgtcaa cgcccagccg aaaaccggat tgaccagacg gaagagaaca
    19081 ccacgatggc ggcggattgg gtggttttgc ctttgcgcgt catttccatg cccgcttcca
    19141 aaagcggtaa gtatacgcct acgatcaagg ctacgctcaa taccggctgc caaatcgcca
    19201 agtccatcgg atagccccac aacccggcga taatacataa aaccgccgtt aaaaccgcac
    19261 cgcccggaat ggggcgtttg gcaatcgatg ccggcacgat ataagttccc caagaagagg
    19321 taaaatttgc accccctaaa atagaaccca ctgcttgacg gacagaacaa cttgtcatgg
    19381 tgtcgtctat attcatcaat accttatcgg ttttttccgg atagctgatt ttttggaaca
    19441 cttgatgtcc taaaaaatcg ggcgaccaca ttgcaacagc caataccgca aatggaaaga
    19501 caaccaaaaa actttctgcc gtcggcaacc ccagatgcca gccgctgttt tcaccctacc
    19561 aataagcagg actcattgga ggcaggccgg gggcggtgtg aaactcaaac ggcgcaccca
    19621 atgcaaatgc cgccacaccg gcaatcaagc atcccaaagg cacggccaac cagcgttttt
    19681 tccaatgctc caacaaagcg tacatcacaa tcgttacaat aatgacggta aaagcgatgt
    19741 agggcatatt aaaaccgcct gcccacgaaa acaatttttt tacctgcccc gtcgtgccga
    19801 taaagcccaa atagagtaat aatccgccgc atacgccgtt gcttgtcagc ttcgccataa
    19861 tactgccgcc gcgaaataaa gccatcagca gacctaaaac cgcaatcgaa atgccgaacg
    19921 ccaaaggatg cccgcctgcc gacacaacga tgggaatcat cggaatcagc ggcccgtgcg
    19981 taccgggcag gttggcgccg ggcagaaaaa agcccgatac caataagata aacgcggcgg
    20041 cgattaaaag ctcatagcgc acattttcca gtacaaagct gtcaggcagc cccaaaggtg
    20101 cggcaaacgc cgccgccccc ccaccatcac cacttttcca atcgttcccg ccatcgcagg
    20161 aatcaaatcc tcccactcga agcggtaatc gcgaaagggc aggttgggcc gccagcgttt
    20221 tggttgcata atctgcaatt catgttccaa atattcgtcc cgcgtcgcaa attccgaagc
    20281 cggacggtgt aaatcccgat aagtcccatt atgtttttcc ataaccttcc tccttatata
    20341 tcgcgcctcg taaaaggggg cgcatgactt ttctttttga tacgggctgc gttcggaagc
    20401 cgtaacccca tttaaagccc aaacaggcaa taaaaccaat ttttttttaa taatcatcat
    20461 ccggaaaact gatacaattt acaaaccact tgattaaaaa gttaattttc agcaacaatc
    20521 cgcctaaaag atttcgattg cacaaatata gaaaacatcc gcacaaggag ggatacatgg
    20581 atgccgtaca attaaaatca tttgtcgccg tcgcgcacga gggcaacctt acccaaaccg
    20641 ccaaacgact tttcctttcc cagcccgccg tttccgccca aattaaagcc cttgaagaat
    20701 atgtcggcac gccgctgttc aggagcacgg ggaaaggcat ggtattgacg cgggcgggcg
    20761 aaatactgtt gcccgaagcg gaatccctgc tgcaatacaa acacaagttg gagtattttg
    20821 ccaaaacgct ggcaggcgat tattcggaag agaccagttt gggcattatc caccccatcg
    20881 attcggcaaa actcgtcgcg ctgacggaca atatcggtca aacagccccc caaaacgcgc
    20941 ctgcacatcc aatacggaat gagcggcgaa atcctctcgc gcatccaaca caaaaccctg
    21001 cacggcggct ttatactcgg caacgccgcc caaggcggca tccgcagcgt attcctgcaa
    21061 aacctgacct acgcgctgat ttgcccgcaa agccaatatt cccggctgac ccgctccctt
    21121 ccgcagagcc tgcaagaatg cgtatggata gaaatgtcgg gcgtgtccgg aagtaggaag
    21181 cacctgcacc agttttggcg cagcaaccgg ctctcaccca aaaaaacaga tattgtgcga
    21241 ctacccccaa acccttatcg atttggttgc aggcggtata ggtgtggcaa tggtgcaggg
    21301 aaacaaagcc gaagcggcgg caaaagaagg cgcgggcgtg gctattatcg aatcgtgccg
    21361 ccacagtatg ccgctcaatt tcatttatgc ggaagaatac gaggataatc cccacgtctc
    21421 actcctgctc gagtgcgttg aaaaagtatg gggagtgcag gcggtgcagc cgcccgttgt
    21481 ctcggacaac tgaaataaat cctgctttgc tgattgtttt agaatagaaa tttgaatttt
    21541 atcacgctga aaacactgaa aacgccatcc gcattctctc gaatacggct taaaatgccc
    21601 tttggaaatg ccgttaaact gacgcaaatg ccgttttgca caattccaaa agttttcaat
    21661 tccgttaatg caattttgcc gtttggcgaa atgcgtgctg ttccgtaaaa gccaaaaaaa
    21721 ccttgcgggg cttgcacgcc cccatctgcc gcgatccaag tttgaacaaa atattggaag
    21781 ggtttgctgt tgaaacggga atcgggcagg caatgtgtgg caataaaatg gggcctgccg
    21841 aataatggcg cggccccgat atcattcagt caaactgatg ggaaacggat aagcggatgt
    21901 tgtacgatcc ttttttcccg ccggaaaact cttttttccg catcggcaaa gacattcgtt
    21961 ttttttgcct gtccgcgcat tgtagcccaa tccggcggaa taccaagtcc ccctcgtatt
    22021 tcatttttga gcataatgac ttttgccctc aaatgcctta aaggaacgac tgcccaaaaa
    22081 ttcatgaaag atattgcctt tgatatagaa aagttttttg cctccgtcaa attttctcac
    22141 gacatccaaa cccaagcggc ctatcaggct gtttgcgtgg cttaaattga cgtttatgcc
    22201 gttatcgatc cgatagccgg tacctctcaa ataggaatat tgcaactgta cttgcggtgt
    22261 aatccgccac aatttatcgt catccaaaaa tttacggcgg ccgtattcgg tgctgataga
    22321 gaatagggtg ttgctatatt ttcccttgcc ccataattgc cccatatgat ttcgtgcaat
    22381 cacacggtta cgcagttttc ctatttttaa tacattgtcg atataccctg tttcgtctcc
    22441 ttctttatct atgtattggg tagtattgta cagagccaat tcatatttcc tgattttgtc
    22501 cttgccaaac gtattccata atgatgtcct gccgtggccg tagtcgagac ttatgcccca
    22561 tttgcgtttt ttctcctgct cgttgaggct gaatcggtca tatccgattt caaaatcgtt
    22621 gttatgaaac ctaaacgcat tggattgaat cacacgggta tgcgtcagcc ttatccaatc
    22681 gccttctttt ttatccaacg ttgaaaaagc ccgctcccct tcgcgtttgg cataagtatc
    22741 caaagtgaac gcataatgtg cggttgaatc gaacatatca ttgatatttt tgacatttcg
    22801 gctcggattg ccggtagcct gtttgaccaa atagacattt tgcctgttgt cggtataggt
    22861 tttattgaca taatcgtctc cagctttttc cgccgtcatt tcagagccgt taaaggattt
    22921 attatagtca tccttgttgt ttttatcgcc gttatgtgcg gagtattcga cagtcagggc
    22981 atcctccata aggtgcgtat cgtcaatata ttttttaccg ttcacaaatt cattttttga
    23041 gtttgttact gtagcgaaac gcaaacgttc gccgtaattc atactgtcga gcacttcgga
    23101 cagatttttg acgacgactt cttgcggtgt cgaagtgcct tttttcacat agagcatatc
    23161 gctgtgaatg ccgtctttat tcagatgcat caagaattta ttggcacccg ccaattcacc
    23221 gatatgtaag gcgcgtccac ttgttttagc atcattatta aaatcgatag tgccttgtcc
    23281 ttccaattta tccagccagc tttgacctgt catcgtccat aatgcgtctt tggctaaatt
    23341 aaagttaatt tcgcccgcac ttttgctgtc taaatttgca acatagtttt cgaataaatt
    23401 tttatgtttg gaatcggtta agccgctaaa attatccaaa cgtcccacta aacggatgtt
    23461 cggcgtgaaa tctaattcca ctttaccctt atttaccgcc aagacatcgc ctttatagtg
    23521 aatagtgtcg gcaacaatat tcgtgttatt caaaggtttg atggctacca aacccttatc
    23581 agatacggtt gcccctgtga tacgggtatc gtctttataa ttcaaatgaa ttgaaccatc
    23641 ttgccttgca agattggcaa cgttggagtc aatggtaatt tgaccaaatc cacctttgcc
    23701 tttttgtagg cgaaaacatt atatttcggg tttgaaatca cattattttt tccccttgag
    23761 ggaaattgag gagttgttgg agtataagcc ccaaattccg ccttgtacga tattgtcgct
    23821 cttcgcagac aattcgactt tcgcgttttg ccatgaataa atgccgatat ggcgaaatcc
    23881 cccatttaat gccttagatt tttcactgat ttcactctta acgatattat tctcgccaat
    23941 cagtttgacc agcgatttgt cgccataggc aaaaatgccg ttggaacctc gtttgctttt
    24001 ataatatggt gtctccgtta atactttgga attgtttaca taatccgcat ccgcatcgcc
    24061 ggatttcatg gtaacaatat tatcgccgcc ggcttccagt atgattttat ttccatcatc
    24121 ccttgttccg ggtcttgccc ccgttctgtt aatcagagtc gaaatgccgt cgctgctgta
    24181 tgcggcgttt tccacggtaa ttttattgtt ttctttcgcc ttgagggtaa tcgtggcatt
    24241 tccgtgagcg gtaatccctt cttggttcat cgggctgcct cctacttcaa cgatattgtt
    24301 tgccgcactc aaaactacat cgctgtcaat cgtggattga accccccacc ctgtatggtt
    24361 ctggcgaatt attggggtat ttttctttcc aggcattgat taaagccgtc ttggtgtccg
    24421 gatttttaac ccggacaata ttatcctcct cggcttcaag cgttgctttt gcagaatgcg
    24481 acgtgataat gccttttacg gaggcgtggt tttttacatt taaatcaact tcattattcc
    24541 ccttgacggc tttgagttcg atggtgctgg tgccgtcaag ggaaggttgg gtagtattgc
    24601 ttggttgatt attgatgaaa gtacccgtcg tgtgtccgga atagggggag ttggactgga
    24661 ggccatttac gcccaatccc aagccgtcgc cctcagtggt gatggatatt ttattgttat
    24721 tccccgttaa tacggtattg gatttaaaag ccgtttcaat accccttaaa aaacttgtac
    24781 tctcaacttt tcctgccggc ataggctcgg caagcttccc tttaatcgca accacattat
    24841 cgccgtcttc ggcagtcaga ttaacattgg tattgcggag gttgtgaata ccgaaatggt
    24901 gtgtgccttt atgaacgtta acgttcatat tgaacaaatt gcctgttttt gaatggaagg
    24961 atatgctgcc tttgtcattt tcatcggagg gggtgatatt gttgacatac cgcactaaag
    25021 cgttatcgtt cactaacttt tcttgatgtg tggttggatc tgttacctta tcggcaacat
    25081 tcaaattaaa ttgaattttt cctttggcat ccaaatggat atttccggaa tagggttgta
    25141 cgtcaacaga ttgaaaatca ttgttgaaaa taatgtcatg attgcttctc accgataatt
    25201 cgggtttgta ttccaatttt aggtctgcaa aagaacggat gccgtaagaa ccgttgacca
    25261 gtaagtctcc atctgcccct tccccggtgg taataagttc caatttctca taactgttcc
    25321 cctttcttcc aataaggcaa aatagttttg tttactgtaa tcttaacttc cccgcatggt
    25381 taaaacggga cctggtcttt gcgggtccgg ttgcaaagct gcatggatgg catagcgtat
    25441 aaatttcctg aaatttccca attgtacgcc taagatcggc ttagtacaaa cggtggggcg
    25501 gtatccgtat gcggcacgcc gcctttccct gccgtataat catattgtct gaaaacctgt
    25561 tgacggagcc gttatgaccg atttgtttgt ccgagaaccc gacgcaccgc ttgccgaacg
    25621 tctgcgcccg catacgcttg atgatgttat cgggcagcag catttaatcg gcgaaggcaa
    25681 gccgctgcgc gtggcggtgg aaggcgggaa gccgcattct atgttgctgt gggggccgcc
    25741 gggcgtgggc aagacaacgt tggcgcggat tttggcgcag agtttcaatg cccagttttt
    25801 gcctgtttcc gccgtgtttt ccggcgtgaa ggacatccgc ggggcaatcg ataaggctga
    25861 aatcgctttg cagcagggac gcgcgacgat tttgtttgtc gatgaagtcc accgcttcaa
    25921 caaggcgcag caggacgcgt ttttgccgca tgtcgaaagc ggtttgctga cctttatcgg
    25981 cgcaacgacg gaaaatccgt cgtttgaagt caatcccgcg ctgctgagcc gcgcgcaggt
    26041 atatgttttg caatccttgt cttcagacga cctcaagaaa ctgattgcca aagtgttggc
    26101 tttgcccgaa taccgggatt tcacgattga agccgatgtg caagagctgc tcgtcaatac
    26161 cgccgacggt gatgcgcgca gattattgaa tttgttggaa caacttttac gtgccgccga
    26221 cacgcgccgt ctgaaaatcc tgaccaccga atttctcgcc gacagcttgg gcgcgcaaat
    26281 ccgccgtttc gacaaaggcg gcgagagttt ctacaaccaa atctccgccc tgcacaaatc
    26341 cgtgcgcggt tcgcatccga acgccgcgct gtattggttt tgccgtatgc tcgacggcgg
    26401 caccgacccg cgctatctcg cccgccgcat cgtgcgtatc gcttgggaag acatcgggct
    26461 tgccgacccg cgcgccttcc aaatcgccaa cgatgccgcc gccacctttg aacgcttagg
    26521 ctcgcccgaa ggcgaactcg cgctggcgca agccgtgctg taccttgccg ccgccgccaa
    26581 atccaacgcg ggctacaagg catacaacca aatgcgccgc ttcgtcaaag aaaacgccag
    26641 cgacgaagtg cccgtccacc tgcgcaacgc cccgaccaag ctgatgaagg aattgggcta
    26701 cggacgcgaa taccgctacg cccacgacga accgaacgcc tacgccgccg gcgaaagtta
    26761 tatgcccgac ggcttggacg aaccggactt ttaccaaccc gtcccgcgcg ggctggaaat
    26821 caaaatcggc gaaaagctgg catggttgaa atcgctggac gaagaggcat taaaggcaaa
    26881 atgaagcgat gtcgtctgaa gcagaacagt atggcaaagc cggtacaccg aaaaggcgta
    26941 tcggcttttt tcaggtgtta taatccgttt caataaattt tgatgaccca ttacgttacg
    27001 tttatgtccc aaacccacaa cgaaaattcc cgcgtcaaaa tccccgccgt tttgcacctg
    27061 atgcgcttgg gctatgatta tctttcgcta aaaaatgcaa attgggacag acaaaccaat
    27121 atctttcccg aaatctttgt agacagcctc tgccgcatca atcccgattt gccgccggat
    27181 gatgcacgcc gcctgcttgc cgacatacgc ttagagctgg ataacgaaga tttggggcaa
    27241 aaattttacg aacgcctcac caatcaatcc ggcggtaaga agctgatcga tttccaaaat
    27301 tttgacaaca acagtttcca cgttgtaact gagttgcctt gtatcaacgg cgacgaagca
    27361 ttccgccccg acattgccct gctggtaaac ggcatgcctt tggtctttat cgaagtcaaa
    27421 aagcccaaca ataaaggcgg catcggcgaa gagcgggagc gcatgggcaa acgtgcgaaa
    27481 aatcccaaat tccgccgttt tatcaacatc acccaattca tgattttttc caacaacatg
    27541 gaatacgacg acggcgcaac cgagccggca caaggcgcgt tttacgcctc gtctgcttgc
    27601 ggcaagcccg tattcaacta cttccgcgaa gagcataaat taaaccttac cgaattattg
    27661 gacacgcttt cagacgacct tgaaaacaac gttctacaag acaataatct gcccgttatc
    27721 aaacacagcc ccgaatttat cagcaataaa tcgcccgata cgccgaccaa ccgcatcctg
    27781 acttcgctgc tttgccgcga acgtcttgcc tttctcctgc aacacgggct gacttacgtc
    27841 aaagcaagtc aaggttcggt gcaaaaacac atcatgcgtt atccgcaact gtttgccacc
    27901 cttgccattg aaaaacattt atcgaacggc ggtaaaaaag gcgtgatttg gcatacccaa
    27961 ggatcgggga aaaccgcgcc tgcctattac aacacccgtt acctgaccca ctattacgcc
    28021 aaacagggta tcgtgccgaa attctatttc atcgtggaca ggctcgattt attgaaacag
    28081 gcgcagcggg aatttaccgc ccgcgactta atcgtccata ccatcgacag ccgcgaagcc
    28141 ttcgctgccg acatcaaatc cgcccaaacc ctgcacaacc acgcaggcaa accggaaatc
    28201 accgtcgtca acatccaaaa attccaagac gaccccgatg tcgtcgcccg taacgactac
    28261 gacctcgcca ttcagcgcgt ctattttctc gacgaagtac accgcagcta caaccccaaa
    28321 ggctcatttc ttgccaacct taatcagtcc gacgtaaacg ccgtcaaaat cgggctgacc
    28381 ggcacgccgc ttatcggcgt aaccgcaggc aacgtcaaca cccgcgaact cttcggcgac
    28441 tacatccaca aatactatta caacgcctcc attgccgacg gctacaccct gcgcctgata
    28501 cgcgaagaaa tcggcagccg atacaaggcg caattacaag aagcactggc gcaacttgaa
    28561 atcgaaaaag gcagctttga ccgcaaagaa atctacgccc atccgcactt cgtccacccg
    28621 atgcttgact acatcttgga cgacttcgcc aaattcagaa aaaccaacca agacgagagc
    28681 ctcggtgcca tggtcgtttg cgacagcgca gaacaggcac gccagctttt tgagcatttt
    28741 cagacggcct cagaccacaa tttcaccgcc gcgttgatat tgcacgacgt cggcactaag
    28801 gaagaacgcg accaatgggt taaagatttc aaagcgggca aaaccgacat cttgtttgtg
    28861 tacaacatgc ttttgaccgg ctttgacgcg ccgcgcctga aaaagctcta tttgggcaga
    28921 ctgataaaag cccataacct tttacaaacc ctgacccgcg tcaaccgcac ctacaaatct
    28981 taccgctacg gctatgtcgt cgattttgcc gatatcgaac gcgaatttga caaaaccaac
    29041 cgcgcctatt gggacgaact ttccaacgaa ttgggcgacg aaatcggcag ttacagccag
    29101 ctattcaaaa ccgccgaaga aatcgaacag gaaattgcag acattaaaaa cgccctgttt
    29161 gatttcgata ccgaaaacgc cgaagaattt tgcagccaaa ttagccaaat agaagacaaa
    29221 aaacagcttc tggccttaaa aaaagccctg caaaccgcca aagagttgta caacatcctg
    29281 cgcctgcaag gcagccacga atttcttgcg catctggatt ttgacaagct caatcttttg
    29341 taccgcgaaa ccgccgcccg cttggatacc ttaaaccttg ccgaaaaatt acagcaaggc
    29401 gacaccgccc atttgctcaa tgaagcgctg gaagacgttt actttcaatt cgtcaaaatc
    29461 ggcgaggcgg aactcaaact tgccgacgat ttaaaagaca tcatgcgcaa agtgcgcgaa
    29521 gggctggcgg gcaacttcga ccaagacgac cttgaataca tcagcctgcg cgaagagctg
    29581 gaacgcatct tcaagaaaaa gaacctcgcc gaagtcgggc aggaagaaat gcaggcaaac
    29641 attgccacac tccaaaccgt ctataccaaa atcaaagagc cgaaccgaca aaacgacctc
    29701 ttgcggcaca aatacggcgg cgacgccaaa tacgcacgta tccacaagcg gttgatggaa
    29761 aacgccgccc tctacggaga caaactcaaa gtcttcaacg cgctaaacgg cgtcaaaacc
    29821 gacgccgacc aaagagtgct ggatatggag cagattttgg acaaccagaa ttactttgaa
    29881 aaacaaatgc agggcatcgt attaaaacgc tttagaacgg aacagcaatt tcctgttcaa
    29941 cccgcagaca tccagaccat caaccgcctg ttggtacggg aatatttaaa agaaagcggg
    30001 cggatttgag aagtagattt tcaggtagcc ttaaccattt atagatacag gaaagctatg
    30061 cacaacaacg acatcatcat ctacaccacg gaagacggac tatccgaatt taccctgcgc
    30121 gagcttgacg gcgaactatg gcttgcccaa aaagaaattg ccgaactcta ccaaaccagc
    30181 aaacaaaaca tcggtaaaca catcaaagcc atatttgcag aacaagaact ggacgattca
    30241 gttgtaaact ttcagtttac aactgccgca gacggcaaga actaccgatg aaatgttttt
    30301 ttcagacggc ctttgaagag ttttgtaacg gaacatcatg aatcatttta agaaacaaca
    30361 aattcaaaat attgctgatt ttaatccgag agagcaacta gccaagggag cattggcaaa
    30421 aagtgttccg atggcaatgt tgaaagaatt tcaacgacaa attacgggtt acgaaatcaa
    30481 ggcatttaac ggtggagcta agtttcgcaa tggtgatacc ttattagcta agattacccc
    30541 gtgtttggaa aatgggaaaa ccgcttttgt agatatttta gatgatggag aagtggcttt
    30601 cggttctacc gaatttatcg tgttaagagc aaaaaatgaa accaatcccg aatttctcta
    30661 ttattttgca atcagtcccg attttagaaa acgagcgatt gaatgtatgg aaggaacttc
    30721 cggcaggcaa cgtgtcaatg aaaatgcatt aaaaacactg gagttgccta ttccagaacc
    30781 tcaaatccaa caatccatcg ccgccgtcct atccgctttg gacaaaaaaa tcgccctgaa
    30841 caaacaaatc aacacgcgcc tggaagagat ggcgaaaacc ctgtacgact actggtttgt
    30901 gcagttcgat ttccccgacg ccaacggcaa gccctacaaa tcctcaggcg gcgacatggt
    30961 gtttgacgaa accttgaaac gggaaattcc gaaggggtgg ggaagtatag aattacaatc
    31021 ttgcttagca aaaatcccaa atactacaaa aatattaaat aaagatatta aggattttgg
    31081 aaaatatcct gttgttgacc aaagtcaaga ttttatttgt ggatttacca atgatgaaaa
    31141 atctatatta aacccacaag atgctcatat tatttttggt gatcatacaa gaattgttaa
    31201 gttagttaat tttcaatatg cgcggggagc agatggtaca caagtaatat taagcaataa
    31261 tgaacgaatg ccaaactatt tgttttacca aatcataaat caaatagatt tatcaagtta
    31321 tggttatgct agacatttta aattcttaaa agaatttaaa ataattttgc ctagtaaaga
    31381 tatttctcaa aaatataatg aaatagcaaa tactttcttt gttaaagttc gaaataatct
    31441 aaaacaaaac caccacctga cccaactacg cgatttccta ctacccatgc tgatgaacgg
    31501 gcaggtatcc gtaagatgta gcggagcaag agatggataa actatttaaa atattattaa
    31561 atataagtag tacatctttg ctgttatcaa tatattttat taaatcgcaa tattatatat
    31621 tagaccttac ttggttttat cgtgtaattt caaatcattg gatagaacct atttctaagt
    31681 taagtctact tttttatttt ctaataccat ttatcgcaac cgctactgtc ttatggctaa
    31741 gcaaatactt ggggaaggat gaattcaagc aaggagaggt gaaagaatta gaatatgtta
    31801 acgataattt cttacctagc tatcttggtt atttttttgt tgctctaagc atacccgata
    31861 ataatctttt tttgctattt gttatgtatg gaattatttt cttactagtt tcatgctcta
    31921 agtcatttta ttttaaccct gtttttttct tatttggcta tcgattttat caagcaaaaa
    31981 ctgaatcagg gttattatta gtgctgatta gtaaacagga atttagaacg ccacaaagtg
    32041 taagttcgat tgctgtatat cgaattaata attttacttt cttagaaaaa taggagttca
    32101 ataatgaatt ttgttttagc taaaattact tgcaagaaag atttagtaaa aatattatct
    32161 gatgatcata ttttccctga
//
LOCUS       DBJGGI010000017        31739 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.017, whole genome shotgun sequence.
ACCESSION   DBJGGI010000017 DBJGGI010000000
VERSION     DBJGGI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31739
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.017"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..587
                     /locus_tag="ACSIGJ_000781"
     CDS             <1..587
                     /locus_tag="ACSIGJ_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HHQ1329706.1"
                     /translation="AGESVLSKDEASGVTGCKPVTARYGNPYRETVYIEVSDGIGNSQ
                     TLISNRIHSFYSGGKWIKAEDLKAGSRLLSESGRTQTVRKTVVKPKPLKAYNLTVADW
                     HTYFVKGNQAETEGVWVHNSCPPKRTGSSKNEKHGDGGRSQISAESKIAELTNKIIPG
                     MSKNERLKIKQKIKNIAKNANRKTKGEEHGRRGR"
     gene            568..1023
                     /locus_tag="ACSIGJ_000782"
     CDS             568..1023
                     /locus_tag="ACSIGJ_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329707.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     GTEYYESPDSNLLISVKKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHHPKSYGVD
                     KYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
     gene            1092..1469
                     /locus_tag="ACSIGJ_000783"
     CDS             1092..1469
                     /locus_tag="ACSIGJ_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HHQ1329708.1"
                     /translation="MCEFKDFRRNIPCFEEYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRRKYPYPMDIPRDIVIGIGTIIEFLMVPNWKLFTIKSSPWLPDSVGIHERYERFT
                     TMLRYIFTEKDIVNVRFDYYNKK"
     gene            complement(1533..3746)
                     /locus_tag="ACSIGJ_000784"
     CDS             complement(1533..3746)
                     /locus_tag="ACSIGJ_000784"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HHQ1329709.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKNLIGTAWSLA
                     QEHYPADAATPYGEPLPDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATSEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLDILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSGKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSATTIVKQSKIKKGGKTGVLIDGEDGLMTTLAKCCKPAPPDDIAGFVTR
                     ERGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLAGLGDVKGVLSVTRL"
     gene            3889..4530
                     /locus_tag="ACSIGJ_000785"
     CDS             3889..4530
                     /locus_tag="ACSIGJ_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HHQ1329710.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEVVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVCHFDREGRLKDEVRPEIQAWLDKVGGYNNKLVQPRL
                     IKIGLPEFVTPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGTSD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
     gene            complement(4602..5240)
                     /locus_tag="ACSIGJ_000786"
     CDS             complement(4602..5240)
                     /locus_tag="ACSIGJ_000786"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HHQ1329711.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCIAASGP
                     LSNLAMAVLWGVVLVLTPYAGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLPAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            5496..6347
                     /locus_tag="ACSIGJ_000787"
     CDS             5496..6347
                     /locus_tag="ACSIGJ_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HHQ1329712.1"
                     /translation="MDKERILTPAVVFSVALLHLAIVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGGGAPEGAGAPAAPEPQPAPDPPKPVEPPKPVLKPAVTKKADADIQQPKEK
                     PKPEEKPKPEPEPEAKPAPKPAEKPAEKPSEKPAEHSGNASAKAGSEQGNGEGKGTGT
                     KGDGTGRGEGSGKGSGGAKGEHGEGAGGSGGGTGVGSSKGNPLRANGSIPRPAYPALS
                     MENDEQGMVVLSVLVSPGGHVESVKVVKSSGFSRLDNAARKAAQNGHFQANAWTEFKV
                     PVKFELN"
     gene            6413..7075
                     /gene="exbB"
                     /locus_tag="ACSIGJ_000788"
     CDS             6413..7075
                     /gene="exbB"
                     /locus_tag="ACSIGJ_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HHQ1329713.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSTVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            7078..7512
                     /locus_tag="ACSIGJ_000789"
     CDS             7078..7512
                     /locus_tag="ACSIGJ_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HHQ1329714.1"
                     /translation="MAFGSMNSSDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQANKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(8013..8441)
                     /locus_tag="ACSIGJ_000790"
     CDS             complement(8013..8441)
                     /locus_tag="ACSIGJ_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HHQ1329715.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(8506..10254)
                     /locus_tag="ACSIGJ_000791"
     CDS             complement(8506..10254)
                     /locus_tag="ACSIGJ_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HHQ1329716.1"
                     /translation="MIHYKIAPSPLDHEWHILLKFTQDNDFPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHTAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPPEWRIATTLPETGRLVFQTASYDELIDRP
                     VEMGLIEFLDFEAAGIPHTIALNGIYPDFDRDRLVSDIKKICETELAMFSSPAPFEKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDAYTTLLGLFSHEYFHAWNV
                     KSIKPAAFAPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPEFYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTVMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLTDFFQTALYSTEDLPLAEC
                     LATTGVKLTFLPLPRQHGGGYAEHIFPIPPTGDFGARFKQNADHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFAAQWARYHVRAKINIHFFRAGILRQTVLTVQATAADTA
                     ILHITDRNLLENWLFG"
     gene            10580..12013
                     /gene="ccoN"
                     /locus_tag="ACSIGJ_000792"
     CDS             10580..12013
                     /gene="ccoN"
                     /locus_tag="ACSIGJ_000792"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045277 - respiratory chain complex IV
                     [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HHQ1329717.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKVKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYMIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            12040..12651
                     /gene="ccoO"
                     /locus_tag="ACSIGJ_000793"
     CDS             12040..12651
                     /gene="ccoO"
                     /locus_tag="ACSIGJ_000793"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HHQ1329718.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            12656..12826
                     /locus_tag="ACSIGJ_000794"
     CDS             12656..12826
                     /locus_tag="ACSIGJ_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HHQ1329719.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDDAANSIFAE
                     NQDAQDKKSENR"
     gene            12850..14169
                     /gene="ccoP"
                     /locus_tag="ACSIGJ_000795"
     CDS             12850..14169
                     /gene="ccoP"
                     /locus_tag="ACSIGJ_000795"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HHQ1329720.1"
                     /translation="MNATSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPKGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSNKDGKAPVKKAEPAPAAEPAPSAPAEAAQ
                     AASEAKPAAAEPKAEEKAAPAAKADGKQVYETVCAACHGNAIPGIPHVGTKADWADRI
                     KKGKDTLHKHAIEGFNTMPAKGGRGDLSDDEVKAAVDYMVNQSGGKF"
     gene            14513..15922
                     /locus_tag="ACSIGJ_000796"
     CDS             14513..15922
                     /locus_tag="ACSIGJ_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HHQ1329721.1"
                     /translation="MDTQIKTEAGQQNNRHYLSVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVFPQAVVQPLSVQAEAARGAINPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVNTMPRNQGWYHTMDEIHGDMMLGAAGDYLLETAASL
                     TIIMVVSGLYLWWAKQRGIKAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWILELTPMPVS
                     GTTVGENGINPSEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVVFCLAV
                     IFIGISGCVMWWKRRPSGVAGIVPPAQKIKLPVWWAMALPLLLIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRKWFK"
     gene            16023..19229
                     /gene="recC"
                     /locus_tag="ACSIGJ_000797"
     CDS             16023..19229
                     /gene="recC"
                     /locus_tag="ACSIGJ_000797"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HHQ1329722.1"
                     /translation="MFYLYQSNRLESLAALFARIQKVKPLKCALQPEQIVVQSQGMRR
                     YLNTCLARDLGVAANLAFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSA
                     EFQNGAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLAALDKDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMHWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIEPYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPFFYALECVSDLLESRFEVGKVLALLETAPVLRRFGLTGDDLPLL
                     HDMVADLNVRWGLDGEMRGGTDQLFTWKQAADRMILGWMLPEGGNPMWQDVSAWYADV
                     NQTAMFGRFAAFLETLSDIARIWRQPATVGEWVARCRDLLETLFQAGPDDQKAVQNLE
                     NEWVKWQEESTLAGFFGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLVAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMTGTNSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPRPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QLAWSEPHIGEAWEPAEPFEPQHAEQIAETYIGARREGQDFSQTAARIAAESLLPSGE
                     LGKLWQQSFQTAAKQIDTAVLNSPKLPPFPYAIPSDGQILKGSLGNLYRCGQVFYAYG
                     KPNAPQRVAFLLEHLIFCAVMPSEAETRQTFIVQSGETEVLAEIAQDRALQLLSEWMA
                     FFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGSKVSKGQKDYTEVAL
                     VFGNASQNPLEQPLFENLARLLADTLAAAEKREGIGAA"
     gene            complement(19299..20867)
                     /gene="mtrF"
                     /locus_tag="ACSIGJ_000798"
     CDS             complement(19299..20867)
                     /gene="mtrF"
                     /locus_tag="ACSIGJ_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HHQ1329723.1"
                     /translation="MSQTDARRSGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAVG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAVI
                     FHSLGRHPLAGLAAAFADVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMAASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFIALSALLAWSIVPADGILRHPETGLVAGSPFLKSIVVFIFLLFALPGIVYGRIT
                     RSLRGEREVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGAVFLKEVG
                     LAGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPVP"
     gene            21199..21411
                     /locus_tag="ACSIGJ_000799"
     CDS             21199..21411
                     /locus_tag="ACSIGJ_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329724.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENIHAMIGEIKNAI"
     gene            complement(21504..22136)
                     /gene="mtrR"
                     /locus_tag="ACSIGJ_000800"
     CDS             complement(21504..22136)
                     /gene="mtrR"
                     /locus_tag="ACSIGJ_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HHQ1329725.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     DALYWHFKNKEDLFDALFQRICDDIENCIAQDAADAEGGSWTVFRHTLLHFFERLQSN
                     DIHYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSGESFDLGKTAPRIIGIMMDNLENHPCLRRK"
     gene            22387..23625
                     /gene="mtrC"
                     /locus_tag="ACSIGJ_000801"
     CDS             22387..23625
                     /gene="mtrC"
                     /locus_tag="ACSIGJ_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HHQ1329726.1"
                     /translation="MAFYASKAMRAAALAAAVALALSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVSADAISKQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGAIAVGIKFDDGTVYPEKGRLLFADPTVDE
                     STGQITLRAAVSNDQNILMPGLYVRVLMDQVAADNAFIVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGMTGAKKVTPKEWAPSENQA
                     AAPQAGVQTASEAKPASEAK"
     gene            23637..26840
                     /gene="mtrD"
                     /locus_tag="ACSIGJ_000802"
     CDS             23637..26840
                     /gene="mtrD"
                     /locus_tag="ACSIGJ_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HHQ1329727.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHAIYPGASAQVMEGSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNV
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADVGSALSAQNIQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNIYLKDVAKVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERLAVLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMTLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGAAGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFDSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATKERTDATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTASGSDAVAVAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAAGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVRHASKAGITGSDDKQY"
     gene            26893..28296
                     /gene="mtrE"
                     /locus_tag="ACSIGJ_000803"
     CDS             26893..28296
                     /gene="mtrE"
                     /locus_tag="ACSIGJ_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HHQ1329728.1"
                     /translation="MNTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFQNDTSV
                     SSIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANGSRQGSLSGGNVSSSYNVGLGAASYELDLFGRVRSSSEAALQGYFASVANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINRPIPEDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVGTGSVELGGLFKSGTGVWA
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTGK"
     gene            complement(28497..28695)
                     /locus_tag="ACSIGJ_000804"
                     /pseudo
     CDS             complement(28497..28695)
                     /locus_tag="ACSIGJ_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28695..28901)
                     /locus_tag="ACSIGJ_000805"
                     /pseudo
     CDS             complement(28695..28901)
                     /locus_tag="ACSIGJ_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            29251..30030
                     /locus_tag="ACSIGJ_000806"
     CDS             29251..30030
                     /locus_tag="ACSIGJ_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HHQ1329729.1"
                     /translation="MKLVRSQKISDQVLSVLEGRIVEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNILVARQILEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIRFWLEKFEEACGSGNLEHQGEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSILKDREYQSRNRHADTLAQNDLKRVQDWEV"
     gene            complement(30068..30174)
                     /locus_tag="ACSIGJ_000807"
                     /pseudo
     CDS             complement(30068..30174)
                     /locus_tag="ACSIGJ_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(30269..31603)
                     /gene="gdhA"
                     /locus_tag="ACSIGJ_000808"
     CDS             complement(30269..31603)
                     /gene="gdhA"
                     /locus_tag="ACSIGJ_000808"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HHQ1329730.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFTSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSDGFVLFPDIGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCHVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWA
                     REEVDSRLFGIMQSIHESCLKYGKVGDKVNYVNGANIAGFVKVADAMLAQGF"
BASE COUNT         7598 a         7662 c         8807 g         7672 t
ORIGIN      
        1 gagccggcga gagcgtcctc tccaaggacg aggcaagcgg ggtaacggga tgcaaacctg
       61 ttaccgcccg atacggcaat ccgtatcggg aaaccgttta cattgaagtt tcagacggca
      121 tcggcaacag ccaaaccctg atttccaacc gcatccactc gttttattcg ggcggcaaat
      181 ggattaaggc ggaagattta aaagcgggaa gcagactgtt atccgaaagc ggcagaaccc
      241 aaaccgtccg caaaaccgtt gtcaaaccaa aaccgctcaa agcctacaat ctgaccgttg
      301 ccgactggca tacttacttc gtcaagggta atcaggcgga aacggaaggg gtttgggttc
      361 ataattcttg tccgcctaaa agaacaggaa gctccaagaa tgaaaaacat ggagacggcg
      421 gtcgaagtca aatatcagcg gaatcaaaaa ttgctgaatt aacaaataaa attattcccg
      481 gaatgtccaa aaatgaacgc ttaaagatta agcaaaaaat caaaaatatt gccaaaaatg
      541 ccaatcgaaa aacaaaagga gaagagcatg gtcgacgcgg tcgttaaaac tcccgaattt
      601 ttacctttta cttctgtagg aatattccgt tttggtgctg atataacaca gtacaaaaac
      661 atactggaaa cttttatgta tgaacctcct gatgagtttg gaacagaata ttatgaatct
      721 cccgactcaa atttactcat ttccgtcaaa aaaaacaaaa ttatttcgat attttgttat
      781 caggagctat attttatggg cgttaatata ataggtctga attttgaaga ttttaaacag
      841 cttttccatc accctaaatc atacggtgtt gataaatatt atttatcaaa tgaatcttat
      901 ccgacatatg tctatgagtt tgatgaaata ggagtacaag catgggaagc aaaagggaaa
      961 attgttacta tcattgccgg gggcaaagac aattattcga cagaacctta ctacgatgaa
     1021 taacattaca gaaaagaaac aagaaacggt acatttgata aaaacctaaa tcgtattgga
     1081 gattaattat tatgtgcgag ttcaaggatt ttagaagaaa catcccttgt tttgaagagt
     1141 atgacgaaaa ttcatttatt ggtaaatggt atgatgacgg ggtgtgggat gatgaagaat
     1201 attggaagct ggagaatgat ttaatcgagg ttaggagaaa atatccttat ccgatggata
     1261 taccaaggga tatcgtgatt ggaatcggta ccattattga gtttttaatg gttccaaatt
     1321 ggaaactttt tacaattaaa tcttcccctt ggttgcctga tagtgtggga attcatgaac
     1381 gttatgaaag attcacaacg atgctccgtt atatttttac cgagaaagac atagtcaacg
     1441 tgcgatttga ttattacaac aaaaaatagc aaaacccggc aatcaaaatg ccgtctgaag
     1501 cgttatccgg ctttcagacg gcatttttgt atttaaagcc gggtaacgct caatacgcct
     1561 ttgacatcgc cgaggccggc gaggacgcgc gggaggtcgt tgacttgttt gacttcgagc
     1621 gtgaacctca tgctggcttc caagtcgcgg gactgggttt gtacggcggt aacgttgagt
     1681 ttgtggcggg cgagcgcgtc ggatacgtcg cgcaaaagcc cggagcggtc ttgggcgcgg
     1741 atttcgatat cgacggcgaa cacttgccct tcctgcaacg ccgcccaact tgcgtccagt
     1801 actttttcgg gcgcgtgttc ggcaaggtgt cggaaagagg ggcaggtttt gcggtggacg
     1861 gaaatgccgc gctcgcgggt aacgaagccg gcaatatcgt cgggcggcgc gggtttgcag
     1921 catttggcaa gcgtggtcat caagccgtct tcgccgtcga tgagcacgcc ggttttgcca
     1981 ccttttttga ttttggactg tttgacgatg gtggttgcgc tgacgggcac gggcggcggt
     2041 tcgttcagcg tgccgcaggc tttttggatg gcgcggttgg aaatttcgcc ttgtccgacg
     2101 gcggtgtaga ggtcttctgg ctttttgtag ccgagatttt cggcaagctc ttgcaggttg
     2161 ggtttgggcg tgagtttggc aagctgcttg tcgagttgga cacggccttc ttcgcgcacg
     2221 gtgtcggcgt tttgctggcg gatgtaggcg cggattttgc cgatggcctt gccggatttg
     2281 acccagcctt cgtaaagcca gttgacggaa ggatgccctt ctttggcggt aatgatttcg
     2341 acgcgctgtc cgttttcgag cggggtggac agcggcacaa tctgcccttc gactttcgcg
     2401 ccccggcagc ggtcgccgat gctgctgtgc agggcgtagg cgaagtcgat gggggttgcg
     2461 cccgttggca gagagaggac tttgccgtgc ggggtcaaaa cataaatcgt gtcgttgaaa
     2521 agctcggttt tgaaggcggc ggcgaggtct tccttgccgc tttccgccat attttcgcgc
     2581 cagtccaaga gttggcgcaa ccaggcgatt ttttgttcgt aggcggaatc gcctttgccg
     2641 ccttctttgt aacgccagtg ggcggcgaca ccgaattcgt tgaattggtg catatcgaag
     2701 gtgcggattt gcacttccac acctttgtct tccgggccga cgatgacggt gtgcaaactt
     2761 ttataaccgt tgcctttggg gttggcgatg tagtcgtcga actcgccggg aatgggctgc
     2821 cagaggctgt ggacgatgcc cagcgtggtg taacactcgg ggacggtatc gaccagaatc
     2881 cgcacggcgc ggatgtcgaa caggccgtcg aagctgagtt ttttcttcac catttttttg
     2941 taaatggagt agatgtgttt cggacggccg gcgacttcaa agtggatatt gtattttttg
     3001 agttccgtac gcaggatatc gaggaagttt tcgatgtatt cgaggcgttc ggtgcgtttt
     3061 tcgtccaaaa gcagggcgat ttcgcggtat ttttcgggtt cttgatggcg gaagcccaaa
     3121 tcttcgagct gccatttgag ctgccacacg cccaagcggt tggcgagcgg ggcgaagatg
     3181 tcgagggttt ctttggcgac ggcgcgtttt tcggggctgt cgggggcgtt gcttaaaaat
     3241 tgcagggtgc gcgtacgcat cgccagtttg attaatacga cgcggatgtc ggtaaccatc
     3301 gccagcagca ttttccgcat ggtttcggct tgctgcgcgc gctcttcgga agtggcgagg
     3361 ctgtccaccc gggcgaagtg ggtgagcttc tgcacttcgt ccacaccttt gaccagctcg
     3421 gcgacggtgc tgttgcagcg ctcggaaacc aataggttcc aatcggggac gtagcgtccg
     3481 atgtcggcaa gcagggtggc ggcgacggca tcgggcagca ggtcgagttc gtcgaccatt
     3541 tgcgccgcgc cgaggaagtg gtcgggcagc ggctcgccat acggcgtggc ggcatcggca
     3601 ggataatgtt cctgcgccag cgaccatgcg gtaccgatga ggtttttatc gttgtccggc
     3661 agagcggcgc agtagctgtc gaaccattcg cgcaattctt gcagggtcgc gctttgcgtg
     3721 tcttgaatcg ggctgatggc ggtcatgatg ggatcttccg ttccgataaa aacgagttca
     3781 ttgtaacagc ttatttattt acggtgcata ggcgggaaga taaacttgtt tcgggcggcg
     3841 ccgggtgtta aggtagccgg taccgacagg ttgacgagga atattttgat gaaactgtat
     3901 atttacgacc attgcccgtt ttgcgtgcgt gcgcggatgg ctgccgggct gtttggtgct
     3961 gacgttgaag aagtcgtgct ggcaaacgac gacgaggcaa cgccgatcgg tatgatcggt
     4021 gcgaaacaag tgcccgtcct gcaaaaagaa gacggttcgt ttatgggcga gagtttggac
     4081 attgtgtgcc atttcgaccg agaaggccgt ctgaaagacg aagtccgccc cgagatacag
     4141 gcgtggctgg acaaggtagg cggatacaac aacaaattgg ttcagccacg cttgatcaaa
     4201 atcgggctgc ccgaatttgt aacgccggaa gcggtgaaat atttcacaga caaaaaagag
     4261 aaaagcatcg gcagcttttc cgccaatttg aataaaaccg cccaatacct tgaacggata
     4321 aacgcagatt tgcaagagtt ggagaatttg atggacggta cttcagacgg catcaacggt
     4381 ggaatcggta tggaggatat tttggttttc cccgttttga ggaatctgac cgtggtgcgc
     4441 ggcatcgcgt tccctcggaa gacgatggat tatttaatcg gtatgagtga aaaatcaggc
     4501 gtgccgcttt atttcgacag ggcattgtaa aaccaaaaaa aagcggcagg gcaatatgtc
     4561 cttccgcgtt ttttggaacg tttatgccgt ctgagagcca gtcagacgaa catctgcaca
     4621 aacgcaatca ccagccgcac aatcggtgca ataaacgcac ccaaaacccc ggtcagcata
     4681 agcagcagga taatccacgt cccataaggt tcgattttgc ggaacgcttg cgaatatttc
     4741 gccggcagga aggtgtcgat gaaaatgccg ccgtcccaag gcaggatggg gatgatgttg
     4801 agcgcgaaca aaatcgcatt gatcagaata ccgtagtttg ccatttgagc cagcggcatc
     4861 tgatacgccc cgccggcata cggagttagc accaaaacca cgccccacag aacagccatc
     4921 gccagattcg acagcgggcc ggacgcggca atgcaacgcc atgcaaggcg cgggttgcgg
     4981 aagttgcgcg aatcgatagg aatcggacgc gcccagccga acaggaaggg cgtgaacatc
     5041 aaagtaagca gcggtacgat gattgtgccg accaaatcga tatggggcag ggggttcagt
     5101 gtcagcctgc cgtattgttc ggcagtgttg tctccccagt agcgcgccgt atagccgcgc
     5161 gccacctccc tgacggtaat ggcgagcagc acgggcagga cagcaagcag aaacacgccc
     5221 aagtcaaaat tttgaaacat ggttttcctt tgaacagatg tggtgtgcag cccgaaacgg
     5281 ctgacagaca gaagcggcaa tatatcatct aacctgattt tattggaata agaaatcaga
     5341 cggaatcatt ttttctgccg gcccgttatc tgatgatttg ctgtaatacc gcgtttaaac
     5401 acggtttgcc tatgaatgga gcaggcgttc acatgacaac aaattataaa aaataggttg
     5461 caaataggaa ttgttgctat tattatgccc gaattatgga taaagaacga attttaaccc
     5521 ccgcagtcgt gttttccgtc gcactgctgc atttggcaat agtggcattg ctctggcagg
     5581 cgcacaagct gcccgtgata gagtcaggaa atgttatcga atttgtcgat ttgggcgatt
     5641 ttggcggagg gggcggcgca cccgaaggcg caggcgcgcc tgccgcgccc gaaccgcaac
     5701 ccgcgcccga tccgcccaaa cctgtcgaac cgcccaagcc ggttttgaag ccggcggtta
     5761 caaaaaaggc ggatgcggat attcagcaac ctaaggaaaa gccgaaaccc gaagaaaaac
     5821 ctaagcccga acccgaaccg gaagcgaagc ctgccccgaa accggcggaa aaaccggcag
     5881 agaagccgtc tgaaaaacct gccgaacatt ccggtaatgc ttctgccaaa gccggcagcg
     5941 agcagggtaa tggggaaggt aagggaaccg gtaccaaagg cgacggaacg gggcgcggag
     6001 aaggcagcgg taaaggaagc ggcggtgcca aaggcgaaca cggggaagga gccggcggca
     6061 gcggcggcgg aaccggtgtc ggcagcagca aaggcaatcc tttacgcgcc aacggcagca
     6121 tcccgcgccc ggcctatccc gcactttcta tggaaaatga cgagcagggt atggttgttt
     6181 tgagcgtttt ggtttctccg ggcggtcatg tcgagtccgt caaagtggtg aaaagcagcg
     6241 gtttttcccg tttggacaat gcggcacgca aagcggcgca aaacgggcat tttcaagcca
     6301 atgcctggac ggagtttaaa gtccccgtca agtttgaatt gaattaggcg gttgtcgcgg
     6361 cagtttgcgg cagcttggat gtttgattgg ttttttcagt gttggaatca ttatgaattt
     6421 gaaattagtg tttgaatcgg gcgatcccgt cctgatcggt gtgtttgtgt tgatgctgtt
     6481 gatgagtacc gtaacgtggt gtctggttgt cttgcgctgc atcaagctgt atcgggcgcg
     6541 caaagggaat gccgccgtca aacggcatat gcgcgatact ttgtcgctga acgacgcggt
     6601 cgaaaaagtg cgcgccgtcg atgcgccttt gtccaaactg gcgcaagagg cattgcagtc
     6661 ttaccgcaac taccgccgaa acgaagcgtc cgaactggcg caggctttgc cgttgaacga
     6721 gtatttggtc attcaaatcc gcaacagtat ggcgcagatt atgcgccggt ttgattacgg
     6781 gatgaccgcg cttgcctcca tcggcgcgac cgcgccgttt atcgggttgt tcggcacggt
     6841 ttgggggatt taccacgccc tgatcaatat cgggcaaagc gggcagatga gtattgcggc
     6901 ggttgccggc ccgattggcg aggcactggt ggcgacggcg gcgggtttgt tcgtggcgat
     6961 tccggcggtg ttggcataca acttcctcaa tcgcggcaca aaaatactga cccaggattt
     7021 ggatgcgatg gcgcacgatt tgcacgtccg cctgcttaat caaaaggata gctgattatg
     7081 gcatttggtt cgatgaattc cagcgacgat tctccgatgt ccgacatcaa cgttacgccg
     7141 ttggtggacg tgatgctggt gttgctgatt gtgtttatga ttactatgcc ggtgctgacg
     7201 cattccatcc ctttggaact gccgaccgcg tccgagcagg caaacaagca ggacaaacag
     7261 cctaaagacc ccctgcgcct gacgattgat gcgaacggcg gctattatgt cggcggggat
     7321 tctgcaagca aagtggaaat cggggaagtg gaaagccgtc tgaaagccgc caaggagcag
     7381 aatgaaaacg tgattgtggc gattgcggca gacaaggcgg tggaatacga ttatgtaaac
     7441 aaagctttag aagccgcccg tcaggcagga attaccaaaa tcggttttgt aaccgaaacc
     7501 aaggcgcaat aaagttcgac gcttgaacga atgccgcctg aaagtctttc agacggcatt
     7561 tcttatgttt aatcagttat atatagttga ttaaaacaaa aatgagacga cagagcaccg
     7621 tctccatttc tgcgcaggcg ggaatacaaa catctgattt ttcttttatt ttcaaacatt
     7681 actgaaatgg gtaagtctgg agtcctgcct gcgcgagaat aatgaaaatg tgcatttcta
     7741 atttttatag tggattaaca aaaatcagga caaggcaacg aagccgtaga cagtacaaat
     7801 agtacggaac cgattcactt ggtgcttcag caccttagag aatcgttctc tttgagttaa
     7861 ggcgaggcaa cgccgtactg gtttttgtta atccgctata cccactgtaa aaacggagaa
     7921 ggacagagga cggcagtaag gtttgccgtc ctctttttca agttgcggtg gaatgccgct
     7981 tgaaggtgtg gtttaaacgt ttcggcttgt aatcagacgc gggcgttgtc cagccgctct
     8041 ctgaagtttt ccagattcac acctgccgct tgggcgcggc ggatgatttc gtcaaccggc
     8101 ataccttctg ccgcccggcg gaagccccac aggagggagc agcgcgtacc ggtccggcaa
     8161 taggcaagga cgggatattc ggcttgtccg atgagttggc ggaaggtttc gacatcgtgt
     8221 ttttggatgt cgcgtgcggt aacgggttgg tgatggaatc cggtaacgcc tgcttgttcc
     8281 agccactgtt tgatttgggc gaagtcgggt tgcgattctt cttcgcggtc ggggcggttg
     8341 cagatgacgg ttttgatgcc aagttgcgcg atttgttccg catcggcttt ggtcagttgc
     8401 ggggagatgt agagatgttc gtcaagtttg agaatcgcca ttttgtttcc tttgctttgc
     8461 ggttggtgtt cagacggcat tttgtgtgca atgccgtctg aaagtttaac cgaacaacca
     8521 gttttccaac aggttgcgat ctgtgatgtg taggatggca gtatccgccg ccgttgcctg
     8581 aaccgtcaag acggtttgac gcaatatgcc tgcacggaag aagtggatat tgatttttgc
     8641 cctgacgtgg tatcgggccc attgtgcggc aaagtcggtg caggcataac cgtctaaagc
     8701 aatgattttg tcttgcgggc acagtgccgc agattccgca cttccgccgt tgaagacatg
     8761 ggtcaggacg atatggtcgg cgttttgttt gaagcgtgcg ccgaaatcgc ccgtcggcgg
     8821 gatggggaaa atgtgttccg catatccgcc gccgtgttgt cggggaagcg gcaggaaggt
     8881 cagtttcacg cctgtggttg ccaggcattc ggcaagcggc aaatcttcgg tgctgtacaa
     8941 tgccgtctga aaaaaatctg tcaaatccaa gccggtaatt tcctgacaac ggatttgcca
     9001 gtgtttttcc ggaatacccg agtgtgtgtc cctccactcc cgatagagtt tgtccattac
     9061 cgtatcaagg gaatgtctgc cgttgcttcg gttgcgtatt atcagatcaa ggcacaatgc
     9121 ggcaagtgcg cctttttggt agtagctgac gatggcgttg gggctgtttt catccggttt
     9181 gtaaaatttg ttccacgcgg taaaactcga ttccgccaag gtctgcctca aacggccgcg
     9241 ggtttgttgt acgcgcgtaa tgccttgtgc tagcaggttt aaataaaatt cgggcgagat
     9301 ggtgcggctg cgtgccaaaa acaaatcgtc gtaataggat gtaatgcctt cgaatgccca
     9361 tagttgttcg gtatagtttt ctttgtcgag gtcataaggg gcgaatgcgg caggtttgat
     9421 ggatttgacg ttccacgcgt gaaaatattc gtgggagaaa agtccgagca atgtggtgta
     9481 ggcatcgtcg gcatcggtca taccgtacgg cggaaggctg tggcgatcgg cgagcagtgc
     9541 ggtgctgtcg gtgtgttcca aaccgccgta aatatggtcg ccgacgtgga gcaggaacaa
     9601 atatttctca aatggggcag gggaggagaa catcgccagt tctgtttcgc agattttttt
     9661 gatgtccgaa accagcctgt cgcggtcgaa atcgggatat atgccgttta aggcaatcgt
     9721 gtgcggaatg cctgccgcct caaaatctaa aaattcaatc aagcccatct cgacaggtcg
     9781 gtcaatcaat tcgtcataag atgccgtctg aaagacaagc ctccctgttt ccggcagcgt
     9841 tgtggcaata cgccattcgg gtggaatgcc tgtcaattcc aagcggtgcg gcagcgtttc
     9901 cgttccttcg actttcaaaa acaggcacga tccgtcaaaa aaaccgcgtt ctgtcgtcag
     9961 gaaagaacct cgaaccgaca aatcgaatgc atatacggtg tagcggattt gccactcgcc
    10021 gcgtacggcg gcagtgtgcc agcggttttt tgcaatttgt tcgagcggca tggacgtgcc
    10081 gttgcaggat gcatggatag aagtgatgtg gcgggaaaaa tcccgaatca gatagctgcc
    10141 cggcacccaa ttcggaaggc ttatttctat aggaaaatca ttatcttgtg tgaatttcag
    10201 caggatgtgc cattcatggt ctagagggga cggggctatt ttataatgaa tcataactat
    10261 caaatatatc gttaaacggg atggattaaa tgcctttaaa atgcagctta acctacatca
    10321 ataaagtgcc acaaaaaggg aaaattggaa acaaaagcaa tggcggccgg gttgtaattt
    10381 attataacaa tatgatttat atgtaataaa aaacagatta agcaaagggt tcgccggcgg
    10441 cgcgaatgta attccgcttg gcgtaataag gcagatatgt taaatttaga aaaaattacg
    10501 cttgcccgga ttgcacctta aacaggctgc ggaagcggca ggataaaaat ctttattccc
    10561 ataattcatg gagactctca tggacacaca aacttacaac tacaaagtgg tgcgccagtt
    10621 cgccatcatg actgtagttt gggggattgt gggcatgttg gtcggcgtta tcgtcgccgc
    10681 ccagcttttt gctcctgccc tcgacttgtc taatatcgga ccttggttcc acttcggccg
    10741 cctgcgtccg ctgcacacca atgcggttat tttcgcattt ggcggttgcg gtctgatcgg
    10801 cacatcatac tacgttgtcc aacgtacttg taatacccgt cttttcggcg gttggctgcc
    10861 ggcatttacc ttttggggtt ggcaggcggt aatcgttgcc gccgtcgtca gcttccctat
    10921 gggttggacc caaggtaagg aatatgccga actggaatgg ccgattgaca ttctgattac
    10981 tttggtttgg gtggcttacg ccatcgtatt cttcggtacg attgccaaac gcaaggtaaa
    11041 acacatttac gttgccaact ggttctacgg cggctttatt ttggcggtcg cacttttgca
    11101 catcgtcaac aatatcagca tcccggccgg tttgatgaag tcataccccg tctattccgg
    11161 tgcgattgat gctatggttc aatggtggta cgggcataac gccgtgggct tcttcctgac
    11221 tgccggcttc ttgggtatga tgtactattt cgtacccaaa caagcagccc gccccgttta
    11281 ctcctaccgc ctgtccgtcg ttcacttctg ggcgttgatt ttcacctata tgtgggcggg
    11341 tccgcaccat cttcactaca ctgcgctgcc tgactggacg caatctttgg gtatggttct
    11401 gtccttgatt ttgttcgcac cctcttgggg cggtatgatt aacggcatta tgaccttgtc
    11461 cggcgcgtgg gacaaactgc gtacagaccc gattcttaaa ttcctgattg tatccttgtc
    11521 cttctacggt atgtctacct ttgaaggccc gatgatgtcg attaaaacgg tcaatgcatt
    11581 gagccactat acggactgga ccgtcgcgca cgttcatgcg ggtgcgttgg gctgggtagg
    11641 ctttgtaacc atcggttccg tctattacat gattccccgt ctgttcggca aagagcagat
    11701 gcacagcacc aagctggtag aggcgcattt ctggattgcg actatcggtg tggttctgta
    11761 tatcgccgcg atgtggattg caggtgtgat gcagggtttg atgtggagtt ctctgaacga
    11821 tgacggtacg ctgacctact cgtttgtcga atccgtaaaa cgcaccatgc cttactacat
    11881 gatccgtttt gcaggtggtt tattgtatct gagtggcatg tgcattatgg cgtacaacgt
    11941 gtaccgcaca gccatcggtg gtaaagcagt cgatgccgaa atccctgcgg tttcccaaac
    12001 acagcaccac taaaatacta agaaagatag gctaccaaaa tgaaattaca acaattggct
    12061 gaagaaaaaa tcggcgttct gattgtgttc acgctgcttg tagtcagtgt cggtctgttg
    12121 attgaagttg tgcccttggc ctttaccaag gcggcaacac agccggcgcc gggcgtgaag
    12181 ccttacaatg ccctgcaggt tgccggacgc gatatttaca tccgtgaggg ctgttacaac
    12241 tgccactctc aaatgattcg tccgttccgt gcggaaactg agcgttacgg tcattactct
    12301 gttgctggag agtcggttta cgaccatccg ttccaatggg gttccaaacg taccggtcct
    12361 gatttggcac gtgtgggcgg ccgctattcc gacgaatggc accgcatcca cctgctgaat
    12421 ccccgtgatg tcgtgcctga gtccaatatg ccggcattcc cgtggcttgc acgcaataaa
    12481 gtcgatgtcg atgcaaccgt tgccaacatg aaggctttgc gtaaagtagg tactccttac
    12541 agtgatgagg aaattgcgaa agcgcctgag gctttggcaa acaaatccga gctggatgct
    12601 gtagtcgcct atctgcaagg attgggtctg gctttgaaaa acgtaaggta acatcatgga
    12661 tattaacggt attcgcgctc ttttcacggt atggatcttt atctgtttcc tgttagtact
    12721 ctatatcgtc ttcaacaggc ggaataagaa aaactacgat gatgccgcaa acagcatttt
    12781 tgctgaaaac caagatgcgc aagataagaa aagcgaaaac cgttaatatt gtgataacgg
    12841 agcaaaacaa tgaacgcaac atcccaattt accagtaatt tctggaatat atatattgca
    12901 gttattgtct tactgagctt tatcgctttg gcttggctgc tgctgtctca aaatgttgtc
    12961 aaacgtccga agaagggcga agaagtacaa actacgggtc acgagtggga cggcattgcc
    13021 gaatacgaca atccgctgcc ccgctggtgg ttttggctgt gtgttttgac gtggctgttc
    13081 ggtatcggtt atctggttat gtatccgggt gtcggcgact acaaaggtct gctgaaatgg
    13141 accagccata accaatatga aaaagaggtc aaaaaagccg atgagcaata cggcaaactg
    13201 tatgccaagt ttgcggatat gccgattgaa aaagtggcaa aagacccaca ggccaagcaa
    13261 atcgcccaaa acctgtttaa cacttattgt atccagtgcc acggctctga tgccaaaggt
    13321 tctaaaggtt ttccgaatct gaccgatagc gattggttgt ggggcggcga tcccgataaa
    13381 atccacgaga ccatcgaaaa aggccgtgtt gcgactatgc ctgcctgggg tcctgctttg
    13441 ggcgaagaag gcgtgaaaga cgttgcccat tatgtgatgt ccctttcaaa acccaaaggt
    13501 cagtatgatg aggaacgtgc cgcgcgcgga caagccttgt tcagcggtcc gcctgccaac
    13561 tgtttcactt gccacggcga taagggacaa ggtatccaag gtttgggtcc gaacctgact
    13621 gatgacgtgt ggttgtgggg cggtacgcaa aaatccatta tcgaaaccat taccaacggt
    13681 cgcagcagcc aaatgcccgc ttggggacat ttcttggata aagacaaact gcatatcatg
    13741 actgcttatg tatggggtct ttccaataaa gacggtaaag ctccggtgaa aaaagccgag
    13801 cctgcaccgg cggcagaacc cgcaccatct gctcctgcag aagcagcaca agccgcttct
    13861 gaagccaaac ctgccgcagc agaacctaaa gccgaggaaa aagccgcacc tgccgccaaa
    13921 gcggatggca aacaggttta tgaaaccgtt tgtgccgcct gccatggcaa tgcaattcca
    13981 ggtattcccc atgtaggcac caaagccgac tgggccgacc gcatcaaaaa aggtaaggac
    14041 acgttgcaca aacacgcgat cgaaggtttt aatacgatgc ccgcaaaagg aggtcgcggc
    14101 gatttgagcg atgatgaggt caaagctgcg gttgactata tggtcaacca gtcaggcggt
    14161 aagttctaac cgttgttttc cgactttgtc ggatgcagaa atccggattc atatcgaatc
    14221 cggatttttt attttgaacg gaaatcctgt ttttgattta tgccgtctga aagtggggat
    14281 tttgatgctt aagcagtcag accgactgat atttttgtat ggtttaaaga atggtgcagt
    14341 tgggtgtcgg acagcattgt ttgatgcttt cagacggcat ataggtttgt ttcttcaaaa
    14401 ttgaatcttt tttcatatgt tgcaaaaaaa ttgattttag tcataaagaa ttagtcttta
    14461 ttgtttataa tcgcgcatca tcacgatagc cttaatttca aaaggacgat taatggatac
    14521 acaaatcaaa actgaagccg gtcagcagaa taaccggcat tatctgagcg tttggaggtg
    14581 gcatttttat gccggtttat tggttatgcc cttcttgacc ctgcttgccg ttacaggttt
    14641 gggtatgctg ttgtttgcca atattaccgg taaagaggga gagcgtattc acgttttccc
    14701 tcaggcagtg gtgcaacccc tctctgttca ggcagaagcg gcacgcggtg ccattaatcc
    14761 ggagacttcg tccgtcgtcc aatatattgc accgcgtgcc gatgatatgg ttgctgtgtt
    14821 ccgtgtcaac aatgagggta aggcaaccat ggttgcggtc gatccttata cggcaaaagt
    14881 ggtcaacacc atgccgcgca atcagggttg gtatcacact atggatgaaa tccacggcga
    14941 tatgatgctc ggtgcggcag gcgattatct tttggaaacg gcagcttcac tgaccattat
    15001 tatggttgtc agcggcttgt acctttggtg ggcgaaacag cgcggcatta aagcgatgct
    15061 gctgccgcca aaaggcaggg cgcgttcttg gtggcggaat ctgcacggcg cgtttggaac
    15121 ttgggtatcg ttgattttac tgttgttctg cctgtcgggt attgcttggg caggtatttg
    15181 gggcggcaag ttcgtgcagg cttggagtca gttcccggcc ggcaaatggg gtgtcgaacc
    15241 gaaccccgtt tcagtcgtgc cgacccacgg cgaggtattg aatgacggca aggttaagga
    15301 agtgccgtgg attttggagc ttacgcctat gcctgtctca gggacgactg tgggtgaaaa
    15361 cggcattaac cccagcgagc cgatgacatt ggaaaccgtc gaccgtttcg cgcgggaaat
    15421 cggtttcaaa gggcgttatc agttgaattt gcccaaaggc gaggacgggg tatggacttt
    15481 gtcgcaggat tctatgagtt atgacatgat cagcccgttt gccgaccgca cggtgcatat
    15541 cgaccagtac agcggcaaga ttcttgccga catccgtttt gacgattaca acccgttcgg
    15601 caaatttatg gcggcaagca ttgcgctgca tatggggact ttgggctggt ggagcgtgtt
    15661 ggcgaacgtc gtgttctgcc ttgccgtgat ttttatcggc atcagcggct gcgtgatgtg
    15721 gtggaaacgc cgtccgtccg gcgtggcggg cattgttcct ccggcgcaaa aaatcaaact
    15781 gcccgtctgg tgggcgatgg cattgccgct gctgttgatt gcactgcttt tcccgaccgc
    15841 gctgcttgcc attgccgtga tttggctgtt ggataccttg ctgctgtcgc ggattcctgt
    15901 gttgaggaaa tggtttaaat gacccgatgc cgtctgaagc gttcagacgg cattttgttt
    15961 gcgggcggct tgcccgagaa gctatataat tccgcatcac tgtcattttg aaaaagcaag
    16021 ccatgtttta cctgtaccaa tccaaccgcc tcgaatcgct cgctgcactg tttgcccgca
    16081 ttcaaaaagt caaaccgctg aaatgtgcct tgcagcccga acagattgtt gtgcagagcc
    16141 aggggatgcg ccgctacctc aacacctgcc tcgcccgcga tttgggcgtg gcggcgaatt
    16201 tggcgttcag cctgcccgcc ggcctgacgt ggaagttgat gaaaaaactg attcccggta
    16261 ttccggaact cagcccgttt gcacccgaag tcatgcgctg gcggctgctg gatttgttcc
    16321 gcagcgcaga atttcaaaac ggagcggaat ttgaagatgt gaggaatgtg ctgcaagact
    16381 atctgggcag cggagaatcg gcggattacc agcttgcggg acagcttgca gacatattcg
    16441 accaatacct cgtgtaccgt cctcagtgga tagacgcttg gcagcagggc aggatactcg
    16501 gtttgggcga cgacgaaatc tggcagtcca aactgtggcg ttacctcgac gacggcaggc
    16561 agagcgcgcc gcaccgtgtc gcgttgtggg aaaaactgct tgccgcgttg gacaaggata
    16621 agctgcccga gcgttatttc gtgttcggca tttccacgat ggcgccgatg tatttgcagc
    16681 ttttgcacaa gctgtccgaa cattgcgacg tgttcgtgtt cgcactcaat ccgagcggga
    16741 tgcattgggg caacgtcatc gaagcggcgc aaatcctcaa aggcggcggc gatcccgatc
    16801 tgacgcaggc agggcatccg ctgcttgcct cattgggcaa gcaggggcgc gactttttcg
    16861 actttttgaa cgaaatggaa atagaaggcg aaacgccggt atttgaagaa ggcggacgcg
    16921 atacgctttt gcacgccctg caaaccgata ttcaaaacct gaaaatgccg tctgaaaatg
    16981 tgggaagcgt caacacgggc gacggctcga tacgcatcgt ctccgcacac agccctttgc
    17041 gcgaattgca gatactcaaa gacaagctgt tgaaaattct gcatgaacat cccgattggc
    17101 agccgcatga tatcgccgta ttaaccccga acatcgaacc ctatacgcct tttatcgaag
    17161 ccgtgttcgg acaggcgcag cccggcgcgc aggcattgcc gtattccgtt tcggacgtga
    17221 aaatcagccg ccggcaaccg tttttttatg cgttggagtg cgtgtccgac ttgctggaaa
    17281 gccgttttga agtcggcaaa gtgctcgcgc ttttggaaac cgcgcccgtg ttgcgccgtt
    17341 tcggactgac cggggacgat ttaccgcttt tgcacgacat ggttgccgat ttgaacgtcc
    17401 gctggggttt ggacggagaa atgcgcggcg gcacggatca gctgtttacc tggaagcagg
    17461 cggcagaccg tatgatattg ggctggatgc tgcccgaagg cggcaacccg atgtggcagg
    17521 atgtcagcgc gtggtatgcc gacgtgaatc aaaccgccat gttcggacgt tttgccgcct
    17581 tcctcgaaac cctgtcggat attgcccgga tatggcggca acctgcgacg gtcggcgaat
    17641 gggtggcgcg ttgccgggat ttgcttgaaa cattgtttca agccgggccc gatgaccaaa
    17701 aggcagtcca aaacctcgaa aacgaatggg tcaaatggca ggaagagagc actttggctg
    17761 ggtttttcgg acagttgccg caacaaaccg tcatccgcca tatccgccgc ttcctcgaca
    17821 gcgaaagcga ggcaggcttt ttacgcggcg gcatcacctt ttgcagtatg gtgccgatgc
    17881 ggagcctgcc gttcaaagtc atctgcctgt tgggtttgaa cgacggagat tttccccgca
    17941 ataccaaagc cgccgtattc gacctggttg ccaaacatcc cgccaaaggc gaccgcgccc
    18001 gccgcgacga cgaccgctac ctgttcctcg aagccctcat cagcgcgcgc gaaatcctgt
    18061 atttgtccta catcgggcgc gacatccgca aagacgaaga gcttgccccg tcttccctgt
    18121 tgggcgaact catcgacaca gttgccgcca tgaccggcac aaacagccgc caacttgcac
    18181 aaaattggat agaacagcat ccgctgcaag ccttctcgcg ccgatatttc caagaaggcg
    18241 gacgttcaga cggcatattc ggcacgcgta ccgactacgc cgccgcgctc ggacaaacgc
    18301 ccgaaccgcc gcgccctttt ttcgaccaac ccgtagaaaa cgccgaaccc gttgccgaaa
    18361 tcggacagga cgaatttatc cgcttctggc gcaaccccgt caaagtatgg ctgcagcagc
    18421 ttgcgtggag cgaaccccat atcggcgaag cctgggagcc tgccgaaccc ttcgagccgc
    18481 aacacgcgga gcaaatcgcc gaaacctata tcggggcgcg ccgcgaagga caagattttt
    18541 cccaaaccgc cgcccgtatc gcggcggaaa gcctgctgcc ttccggagaa ttgggaaaac
    18601 tttggcagca gtccttccaa accgccgcca aacaaatcga cacggcggtt ttaaacagcc
    18661 ccaaactgcc cccgtttcca tacgccatac cgtcggacgg gcaaatcctg aaaggcagct
    18721 tgggcaatct gtaccgctgc ggtcaggtgt tttacgccta cggcaaaccc aatgcgccgc
    18781 aacgtgtggc ttttttgttg gaacacctga tattttgcgc cgttatgccg tctgaagccg
    18841 aaacgcggca aacctttatc gtccaatccg gagaaacaga agtattggcg gaaatcgcgc
    18901 aagacagggc attgcagctg ttgtcggaat ggatggcgtt tttcaatatc gggcaaaacc
    18961 gcccgctgcc gttttttgcc aaaacctcgc ttgccgccgc cgaagcgttt gcccaaaaac
    19021 aagattggga agccgccctg aaaaaagccc aaaccgccta ttacggcagc aaagtcagca
    19081 aagggcagaa agactatacc gaagtcgccc tcgtgttcgg caacgcaagc caaaacccgc
    19141 tcgaacagcc cctgtttgaa aacctcgccc gcctgctcgc cgacacgctt gccgcagcgg
    19201 aaaagaggga agggatcgga gcagcctgac gggatagaaa ggcaaatgcc gtctgaaaac
    19261 agaaaaaagg ttttcagacg gcattttgtt tatcgtgttt aaggcaccgg atagaatgtg
    19321 ggtgcgccgg gaccgacggg caggcccaaa acaaataccc aaatgcagaa taaggcgatc
    19381 catgcaatta agaagaaagc ggaatacggc aacatcatag aaatcagcgt gcctacgccc
    19441 gcatcttttt tgtatttgat taccgtcgcc ataatcagcc cgaaataact catcatcggc
    19501 gtaataatat tggtaacgga atcaccgatg cggtaagcgg cttgaatgac ttcgggcgcg
    19561 tagccggcca acatcagcat agggacgaaa atcggtgcgg ttaccgccca ttgcgcggaa
    19621 gcggagccta tcatcagatt gataaaagca caaattaaaa taaaaccgat aaacaacacg
    19681 ctgccggcca agccgacttc ttttaagaac accgcccctt taacggcaat atattgccca
    19741 atattcgtcc aattaaaaaa tgcgacaaac tgtgcggcaa aaaagatgat gaccaaataa
    19801 agtcccaaag tactcatcga ttcggccatc gcattaacga cttcccgttc gccgcgcaaa
    19861 cttcgggtta tccggccata aacaatgccc ggtagcgcaa acaacaagaa aataaaaaca
    19921 acaatcgatt ttaaaaacgg cgaaccggca accaatcctg tttcaggatg acgcaaaata
    19981 ccgtcggcag ggacgatgct ccaagccaat agggcggata aagcaataaa caccacgcct
    20041 gcccaaatta atcctttata ttccaaaggc gtgatttcat tggaatgccg gatgtctttt
    20101 tcttcttgtg acaaatctga ctgataaggg cccaattgcg gttcgacgat tttttcagta
    20161 acaaaataac caatcaaagc aatcacaaac gtactggctg ccataaaaaa ccagttggct
    20221 tcagggccta cgacgtagtc ggggtggatg atttgcgccg cctgttgggt gatgcctgcc
    20281 aagagcggat cgattgtgcc taagaacaga ttggccgaat aaccgccgga aacgtcggcg
    20341 aaagccgcag ccagaccggc gagaggatgg cggccgagcg aatgaaagat gacggcggac
    20401 aaagggatta ggacgacata gcccaattca gaagccgtat tggataaaat ccctgtaaaa
    20461 acaaccataa aagtagtgag tttgcgtggg gattttgtaa gcaataagcg cattaatgcg
    20521 gaaatcaagc ccgatttttc cgcaatcccc acgcccaata aagaaaccaa caccgttccc
    20581 aacggcgcga aaccggtgaa atttttaacg gtatgcgtca ggattttgat caaaccgtcg
    20641 gcatcgagca ggctgacaac gtgaatcaaa ccgtcatcgg cacgtccttt cgccccaaca
    20701 ggacgcggat cggggacgga tagtccgaaa tacgcaccga cggcagaggc aatcagcaat
    20761 aacacaatga aaataataaa aagcgtaacc gggtgcggca acatattgcc cagccattcg
    20821 actgtgcgta aaaatcgtcc gctacgacgc gcgtcggttt gactcatctg cttctcctta
    20881 aaaaaatata ttgagtgcat taaatttttg aatttaacat gaagtgtttt gtaagcaaac
    20941 taaaataatc gtatatgtat ataaccgtaa tagattaaaa cggaaaatgc ccacatcgtt
    21001 attctcataa aggcggtaat ccgaaaactt gacgttaaag ccttatcagg aatgactgga
    21061 actcaaaaaa acgaattccc gcctgcgcgg gaatgacggg attttcggtt tttgtttgca
    21121 agaatgccgg agaaaaatgc agtttacatt cgaatggtat tgttattaat tgtaaataaa
    21181 tattggaagg taaatgatat gaaaaatgaa attcaaaaaa tcatggataa atatgatcca
    21241 tggcatgaag atgattttga gtcgtatgaa gacattgcca aagatgtatc gttgatgaca
    21301 gataaaacgt tcattgaaca ttatttgtta gaagtttatt cagaagaaaa cggacatttt
    21361 gaccaagaga atatccatgc gatgataggg gaaattaaaa atgcaattta gtgaatatgt
    21421 atttttagaa gaatgctttg tgtcattttg atgccgtttg aaagggtttg ttcgtttcag
    21481 acggcattgt taccaaggct tgattatttc cggcgcaggc agggatggtt ttccaagttg
    21541 tccatcatta tcccgatgat gcgcggggcg gttttgccca aatcgaaact ttcgccggaa
    21601 gagaaccaac gccaaatcag cccgtccaac gtcgatttga tgaagatgac cgccgtttcc
    21661 ttgtccaaat cgtcagccaa atcctgattt tccaccgctt cggtcaaaac ggcggtaatt
    21721 ttctcgcgcc agattgcctg atgcttgcgg gcaatggcga taacggcggc gttttgttcc
    21781 gtatgttcgc actttaaaaa caggatgttg tggaatttgt agtggatgtc gttgctttgc
    21841 agccgctcga aaaagtgcag cagcgtgtgg cggaataccg tccaagaacc tccttcggca
    21901 tctgcggcat cttgcgcgat gcagttttcg atgtcgtcgc agatacgttg gaacaacgcg
    21961 tcaaacaagt cttccttatt tttgaaatgc caatagagcg cgtcgcgcgt tacgccggcg
    22021 gcttgggcga tttcgttgag cgaggtgcgg gcaatccctt tgcggtaaaa ggtttccaag
    22081 gcggcaagca tcaggtgttc tttggttttc aaggcttcgg ttttggtttt tctcataatg
    22141 gcgttttcgt ttcgggtcgg tttgacgagg gcggattata aaaaagactt tttatccgtg
    22201 caatcgtgta tgtataatga aacccataaa attgagactg catctcaact ttgaaaacag
    22261 gtataacctg ctttgcaacc cgttcgaaca ttcgggtatc aaagcaggcg gtttttatat
    22321 cctgaaataa gaatgcctgt tgattggcaa aaccctttac atcaaaccag acagatagga
    22381 taaataatgg ctttttatgc ttctaaggcg atgcgtgcgg ccgcgttggc tgccgccgtt
    22441 gcattggcac tgtcgtcttg cggtaaaggc ggagacgcgg cgcagggcgg gcagcctgcg
    22501 ggtcgggaag cccccgcgcc cgtcgtcggc gtcgtaaccg tccatccgca aaccgtcgca
    22561 ttgaccgtcg agttgccggg gcgtttggaa tcgctgcgta ccgccgatgt ccgcgcccaa
    22621 gtcggcggca tcatccaaaa acgcctgttc caagaaggca gttatgtccg cgccgggcag
    22681 ccgctgtatc agatcgacag ttccacttat gaagcaggtc tggaaagcgc gcgcgcgcaa
    22741 ctggcaacgg cacaggcaac gcttgccaaa gcggatgcgg atttggcgcg ttacaaaccg
    22801 ctggtttccg ccgatgccat cagtaaacaa gagtacgatg ctgcggtaac ggcgaaacgt
    22861 tctgccgagg cgggcgttaa agcggcgcag gcggcgatca aatccgccgg catcaatctg
    22921 aaccgttcgc gcattaccgc gccgatttcg ggctttatcg gtcagtccaa agtttccgaa
    22981 ggtacgctgt tgaatgcggg cgatacaact gttttagcca ccatccgcca aaccaatccg
    23041 atgtatgtga acgttaccca gtctgcatcc gaagtgatga aactgcgccg gcagatagcc
    23101 gaaggcaagc tgctggcggc ggatggtgcg attgcggtcg gcatcaaatt tgacgacggt
    23161 acggtttatc ctgaaaaagg ccgcctgctg tttgctgatc cgaccgttga cgaatcgacc
    23221 ggtcagatta ccttgcgcgc cgccgtatcg aacgatcaga atatcctgat gcccggcctg
    23281 tatgtgcgtg tgctgatgga tcaggtggcg gcggataatg cattcatcgt tccacagcag
    23341 gcggtaacgc gcggtgcgaa agataccgtg atgattgtga atgcccaggg cggtatggaa
    23401 ccccgcgagg taacggtcgc gcaacagcag ggtacgaatt ggattgttac gtcgggtctg
    23461 aaggacgggg acaaggtggt tgtggaaggc atcagtatcg ccggtatgac gggggcgaaa
    23521 aaggtaacgc ctaaagaatg ggcgccgtct gaaaaccaag ctgccgcccc tcaagccggc
    23581 gttcagacgg catctgaagc caaacctgct tctgaagcga aataaggaag gcatcgatgg
    23641 ctaaattctt tatcgaccgc cccattttcg cgtgggttat ttcgattttc attattgcgg
    23701 caggtatttt cggcattaaa agcctgccgg tttcgcaata tccgtccgtt gccgccccga
    23761 ccattacttt gcacgccatt tatccgggtg cgtccgcaca agtgatggaa ggcagcgtac
    23821 tttccgtgat cgaacggaat atgaacggcg tggaaggttt ggactatatg tccacttccg
    23881 ccgattcgag cggcagcggc agcgtgagcc tgacctttac gcccgatacc gacgagaatc
    23941 tggcgcaggt ggaagtgcag aacaagcttt ccgaagtatt gagcacgctg ccggcaaccg
    24001 tccagcaata cggcgtaacc gtatccaagg cgcgttccaa tttcctgatg attgtgatgc
    24061 tttcgtcgga tgtgcagtca accgaagaga tgaacgacta cgcgcagcgc aacgtcgttc
    24121 ccgaattgca gcgtatcgaa ggcgtggggc aggtccgcct gttcggcgcg caacgcgcga
    24181 tgcggatttg ggtcgatcct aagaaactgc aaaactacaa tttgtcgttt gccgatgtcg
    24241 gcagcgcgtt gtcggcgcag aatatccaaa tttccgcagg ttccatcggt tcgcttcccg
    24301 ctgtgcgcgg acagacggtt acggctaccg taacggcgca agggcagttg ggtacggcag
    24361 aagaattcgg caacgtcatc ctccgcgcca ataccgacgg ttctaatatt tacctgaagg
    24421 atgtggcgaa agtcggattg ggtatggaag actattcttc ctcaacccgc ctgaacgggg
    24481 taaataccac cggtatggcg gtaatgttgt ccaacagcgg caatgcgatg gcgacggcaa
    24541 aggcggtaaa agaacgcttg gcggtgctgg aaaaatactt tccgcagggt atgagttgga
    24601 aaacccctta cgatacttcc aaattcgttg aaatttcgat tgaaaaagtg attcacactt
    24661 taatcgaagc gatggtgctg gtgtttgtcg taatgtacct cttcctgcaa aacatccgtt
    24721 atacgctgat tccgaccatc gtcgtaccga tttccctgtt gggcggtttc gccttcatct
    24781 cttatatggg gatgtcgatt aacgtattga cgatgtttgc gatgacattg gtcatcggca
    24841 tcgtggtcga tgacgcgatt gtggtggttg aaaacgtcga gcgcattatg gcgggtgaag
    24901 gcctgccgcc caaagaagcg accaaaaaag cgatgggtca gatttcgggc gcggtcatcg
    24961 gtattaccgc cgttctgatt tccgtgttcg tgcctctggc gatgttcagc ggggcggccg
    25021 gcaatattta caaacagttt gccctgacga tggcgtcatc aatcgcattc tccgccttcc
    25081 ttgcgctgac ccttacccct gctttgtgtg ccacaatgtt gaagacaatc ccgaaagggc
    25141 atcacgaaga gaaaaaaggt ttcttcggct ggttcaacaa gaaattcgac agttggacgc
    25201 acggctacga aggccgggtt gccaaagtgc tgcgtaagac tttccgcatg atggttgtct
    25261 atatcggctt ggcggttgtg ggcgtgttcc tgtttatgcg cctgccgact tcgttcctgc
    25321 cgaccgaaga ccaaggcttc gttatggtca gcgtgcaact gcctgcgggt gcgaccaaag
    25381 agcgcaccga tgcgacgcta gcacaagtta cccaactggc gaaaagcatt cccgaaattg
    25441 aaaacatcat taccgtttcc ggcttcagct tttcgggcag cggtcagaat atggcgatgg
    25501 gttttgccat attgaaagat tggaacgaac gtaccgcctc cggcagcgat gccgttgcgg
    25561 ttgccggcaa gctgacgggt atgatgatgg ggacgcttaa agacggtttc ggcatcgccg
    25621 tcgtcccgcc tccgattctg gagttgggca acggttcggg cctgagcatc aacctgcaag
    25681 accgcaacaa taccggccat accgcattgc tggcgaagcg caacgagctg attcagaaaa
    25741 tgcgtgccag cggtttgttt gaccccagca ccgtccgtgc cggcggtttg gaagactcgc
    25801 cgcagttgaa aatcgacatc aaccgtgccg cagcggcggc gcaaggcatt tcgtttgccg
    25861 acatccgcac cgcattggca agcgcgctga gttcgtctta tgtcagcgac ttcccgaacc
    25921 aaggccgtct gcaacgcgtg atggtgcagg ccgacgagga tgcccgtatg cagcctgccg
    25981 atattttgaa cctgaccgtg ccgaacaaat ccggcgtcgc cgtaccgctt tccaccattg
    26041 ctactgtttc ttgggaaaac ggtacggaac agagcgtacg cttcaacggt tatccctcga
    26101 tgaagctgtc cgcttcgcct gcaaccggcg tttccaccgg gcaggctatg gcggcggttc
    26161 aaaaaatggt tgacgaattg ggcggcggtt acagcttcga atggggcgga cagtcgcgcg
    26221 aagaggcaaa aggcggctcg caaaccctga ttttgtacgg tttggcggtt gcagccgtat
    26281 ttttggtact tgccgcgctt tatgaaagct ggtcgattcc gctggcggtc atccttgtga
    26341 ttccgttggg tttgatcggt gcggctgcgg gcgtaaccgg gcgcaacctg tttgaaggac
    26401 tgttgggcag cgttccctcg tttgccaacg acatctactt ccaagtcggt ttcgttactg
    26461 tgatgggttt gagtgcgaaa aacgcgattc tgattatcga atttgccaaa gaccttcaag
    26521 cgcaagggaa aagcgcggtc gaagccgctt tggaggcagc ccgcctgcgt ttccgtccga
    26581 ttatcatgac ctcgtttgcc tttattttgg gcgtggttcc cctgtatatt gccgctggtg
    26641 cgagttctgc cagccagcgc gccatcggca cgaccgtatt ctgggggatg ctcatcggca
    26701 cgctcttgtc cgtgttcctt gttccgcttt tttatgtggt ggtgcgcaaa ttcttcaaag
    26761 aaactgcaca cgagcatgaa atggcggtca gacatgcatc aaaagcgggt ataaccggtt
    26821 cggacgataa acaatattaa gcaaaatacc gtctgagaac cgccggtttt cagacggcat
    26881 caggactttt ttatgaatac tacattgaaa actaccttga cctctgttgc agcagccttt
    26941 gcattgtctg cctgcaccat gattcctcaa tacgagcagc ccaaagtcga agttgcggaa
    27001 accttccaaa acgacacatc ggtttcttcc atccgcgcgg ttgatttggg ttggcatgac
    27061 tattttgccg acccgcgcct gcaaaagctg atcgacatcg cactcgagcg caataccagt
    27121 ttgcgtacag ccgtattgaa cagcgaaatc taccgcaaac aatacatgat cgagcgcaac
    27181 aacctcctgc ccacgcttgc cgccaatgcg aacggctcgc gccaaggcag cttgagcggc
    27241 ggcaatgtca gcagcagcta caatgtcgga ctgggtgcgg catcttacga actcgatctg
    27301 ttcgggcgcg tgcgcagcag cagcgaagca gcactgcaag gctattttgc cagcgttgcc
    27361 aaccgcgatg cggcacattt gagtctgatt gccaccgttg ccaaagccta tttcaacgag
    27421 cgttatgccg aagaagcgat gtctttggcg cagcgtgtct tgaaaacgcg cgaggaaacc
    27481 tacaagctgt ccgaattgcg gtacaaggca ggcgtgattt ccgccgtcgc cctgcgccag
    27541 caggaagcct tgattgaatc tgccaaagcc gattatgccc atgccgcgcg cagccgcgaa
    27601 caggcgcgca atgccttggc aaccttgatt aaccgtccga tacccgaaga cctgcccgcc
    27661 ggtttgccgt tggacaagca gttttttgtt gaaaaactgc ctgccggttt gagttccgaa
    27721 gtattgctcg accgtcccga catccgcgcc gccgaacacg cgctcaaaca ggcaaacgcc
    27781 aatatcggtg cggcgcgcgc cgcctttttc ccgtccatcc gcctgaccgg aagcgtcggt
    27841 acgggttctg tcgaattggg cgggctgttc aaaagcggca cgggcgtttg ggcgttcgcg
    27901 ccgtctatta ccctgccgat ttttacttgg ggaacgaaca aggcgaacct tgatgtggca
    27961 aaactgcgcc aacaggcaca aattgttgcc tatgaatccg ccgtccaatc cgcctttcaa
    28021 gacgtggcaa acgcattggc ggcgcgcgag cagctggata aagcctatga cgctttaagc
    28081 aaacaaagcc gcgcctctaa agaagcgttg cgcttggtcg gactgcgtta caaacacggc
    28141 gtatccggcg cgctcgattt gctcgatgcg gaacgcagca gctattcggc ggaaggtgcg
    28201 gctttgtcgg cacaactgac ccgcgccgaa aaccttgccg atttgtacaa ggcgctcggc
    28261 ggcggattga aacgggatac ccaaaccggc aaataaccgc ccgggcaaac aaatgccgtc
    28321 tgaaacgata aggtttcaga cggcatttct ttctttatag tggattaaca aaaaccagta
    28381 cggcgttgcc ccgccttagc tcaaagagaa cgattcccta aggcgcccaa gcaccgggcg
    28441 aatcggttcc gtactatttg tactgtctgc ggcccgccgc cttgtcctga tttttgttaa
    28501 tccactatat gtttgacggt tgcggtcagg ctgcgcggcg tgccgtaaac gtgaatgtcg
    28561 ggcatcgtgc ggcagcgttt gttaaagacg ttttcaaagt ccaaaccgat gcgggtggat
    28621 ttgccgattt tgtaatgcgc ggtcaagtcc agtgtagcgt atggggcgtt gggtcaatgc
    28681 ggttgcggcg acagggaaga aaccgccata cccgtcggca tcccgaagcc gaatacggca
    28741 acggcaagcg caatcagcag cgtataaagc cccgcctgat agcctttcag cttcaggacg
    28801 gtcagcgcgg caaagaaaaa gacgatgggt aagagtgcgg cggcggcagt cagatacagg
    28861 ctgccgccga ttgccgtgta gttttgaacc caagtttcca taattgaaca tctccgaaaa
    28921 tatttttcta atcgtcggca atagtggtca aaccaattaa agcaacgttg cattacttta
    28981 cgaaacttta atattcaggt caatatattt ttgggcggtt cggcagattt gaatcggagc
    29041 tttcatttaa atcccgttaa aacaaatatt tgcatgaaca aaaattgtag tttggtgtag
    29101 ttttttcctg tgtttcgggg gcggtgcgag gtaagtgccg ttgacggcgg gaacggcgtg
    29161 tcattagaat ccgccctgat tggccagtcc aatttgatgt ttgatgtatt tcggaagggt
    29221 tctgcaggga atctttataa aggaggagtg atgaaactgg taaggtctca gaaaatcagc
    29281 gatcaggtat tgtcggtatt ggaaggccgc atcgtggaag gggtttacgc ggaagggggc
    29341 aagattccgc ccgaacgcgt tttggcggaa gagttcggcg tttcgcgtcc gtcggtccgg
    29401 tcggcattga atattttggt tgcccgtcag attttggaag cgaggcaggg cgatggttat
    29461 tatgtttcgg tcaagccgca gcaggatttt ttgcaaagtt ggcaggagct tttgggcaaa
    29521 cattccaatt gggaacagga tgtttttgat ttcagctgcc acatcgaggg ctgtatggcg
    29581 gcattggcgg cagaacgccg gacggatgcc gatttgaagc ggattcggtt ttggcttgaa
    29641 aagtttgagg aagcgtgcgg aagcggcaat ctggaacatc agggcgaagc cgatgtcagc
    29701 ttccatcaga cgattgcgga tgcggcgcac aatttgttgt tcagccattt gtcgggcggt
    29761 ttgttgaaaa tgctgtaccg gcagacgcgc agcagcctta tttatctgaa tcaggaagaa
    29821 gatccgcgtc cgaaactgat ggcgcagcac cgcgtgctgt atgaggcgat atcgaaccgc
    29881 cggccgggcg aggcttcgga ggcggcaaag gctcatttga attatgtggc gagcagcata
    29941 ttgaaagaca gggaatatca gagccgtaac cgccacgcgg atactttggc gcaaaacgat
    30001 ttgaagcgcg tgcaggattg ggaggtatga ataccgttca gacggtctgg catattcgga
    30061 tgtatcggta tagtgaacta aatttaaacc agtacggcgt tgcctcgcct tgccgtacta
    30121 tttgtactgt ctgcggctcg ccgccttgtc ctgatttaaa tttaattcac tatagtatta
    30181 cccgattcga acgggcggat aatgaaatgc cgtctgaaca gcatttgcgg ttcggacggc
    30241 attttgtttg cggggcggag gcgtttgctt agaagccttg cgccagcatc gcatcggcaa
    30301 ctttgacgaa gccggcgatg ttcgcaccgt tgacgtagtt cactttgtcg ccgactttgc
    30361 cgtatttcag gcaggactcg tggatgcttt gcatgatgcc gaacagtcgg ctgtccactt
    30421 cttcgcgcgc ccaagacagg cggatggcgt tttggctcat ttccaagccc gaagttgcca
    30481 cgccgccggc gttggaggct ttgcccggag cgtagaggat gccggctttg ataaattgct
    30541 cgaccgcgcc caaagtcgac ggcatattcg cgccttcggc aacgacgtgg cagccgtttg
    30601 ccaacagggt tttggcggct tcttcgtcca attcgttctg ggtcgcgcag ggcagggcga
    30661 tttcggcggc aacgccccac ggtttttggt tttcaaagta ttgcaaacct tgctctttgg
    30721 cgtaggtggc aacgcgttcg cggcggactt ctttcaattc gatcaaggcg gcgagttgcg
    30781 cttcggtcat accgatgtcg gggaagagga cgaagccgtc ggagtcggag acggtcagca
    30841 ctttcgcacc cagttggatg gctttttcag cggcgtattg cgccacgttg ccggaaccgg
    30901 aaatcagaac gcgtttgcct tcaaaactat cgttgcgggt ttgcagcatc gcttgggcga
    30961 aatagacgca gccgtagccg gtcgcctcgg ggcggatgag gctgccaccc cattccaaac
    31021 ctttgccggt caggacggaa gtgaattcat tgcggatttt tttgtattgt ccgaacaggt
    31081 agccgatttc gcgtccgcct acgccgatgt cgccggccgg aacatcggtg tccgcgccga
    31141 tgtggcggta gagttcggtc ataaaggctt ggcagaagcg cattacttcg gcatcggatt
    31201 tgcctttggg gtcgaagtcg gaaccgcctt tgccgccgcc cataggcagg gtggtcaagg
    31261 cgtttttgaa cacttgttca aaagcgagga atttcaacac gcccaaatcg acggtcgggt
    31321 ggaagcgcag gccgcctttg taagggccga tggcggaact catttgcacg cggtagccgc
    31381 ggttgacttg gacttgtcct ttgtcgtcct gccaggttac gcggaacatc acgacgcgtt
    31441 cgggttcgac gatgcgttcc agcaggcttt gctgggtgta tttcggattt tttgccaaaa
    31501 acggatcaag gctcatgaag acttcttcaa ccgcctgatg gaacggctcc tgattggggt
    31561 tgcgttgttt gaggttggca aacagggtgt tcaggtcagt cattcggata ctccttttgg
    31621 ttgatattgt actaatggga aaacaaattt gtagttggca atataaacct atttttttct
    31681 gatgccaagc ctaattttgc acaaattgtt gacattattt ttcccatatt ttcgatata
//
LOCUS       DBJGGI010000018        31524 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.018, whole genome shotgun sequence.
ACCESSION   DBJGGI010000018 DBJGGI010000000
VERSION     DBJGGI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31524
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.018"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            328..1251
                     /locus_tag="ACSIGJ_000809"
     CDS             328..1251
                     /locus_tag="ACSIGJ_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HHQ1329731.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFLG
                     ADDWRRRLNSGFVVGSDGAALDEHSLFEPGKTMFYYRETGCESEPRIPFEEKILFVDE
                     HLIVVDKPHFLPVIPGGRFLRETLLTRLRLRPELQHLNVEDITPIHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTKTDLPYPLDVVSRLVRGEKFFTTQEAEGV
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(1330..1971)
                     /locus_tag="ACSIGJ_000810"
     CDS             complement(1330..1971)
                     /locus_tag="ACSIGJ_000810"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHQ1329732.1"
                     /translation="MLKPETIHIPGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQVRIPDLLLLIGAAVCHYTDRPEPSAVPNVAKTLMIHG
                     AEDEVVEIEKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(2026..2817)
                     /locus_tag="ACSIGJ_000811"
     CDS             complement(2026..2817)
                     /locus_tag="ACSIGJ_000811"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HHQ1329733.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     RPSELVLSYSRAMMGWLLFAERLPQHITQIGLGGGSFARWTDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGGKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDTLVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLKLPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            2983..3058
                     /locus_tag="ACSIGJ_000812"
     tRNA            2983..3058
                     /locus_tag="ACSIGJ_000812"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3016..3018,aa:Thr,seq:tgt)
     gene            3200..3991
                     /gene="panB"
                     /locus_tag="ACSIGJ_000813"
     CDS             3200..3991
                     /gene="panB"
                     /locus_tag="ACSIGJ_000813"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HHQ1329734.1"
                     /translation="MITVNTLQKMKAAGEKIVMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDEAGAAVVLMECVPAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMRGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            4114..4950
                     /gene="panC"
                     /locus_tag="ACSIGJ_000814"
     CDS             4114..4950
                     /gene="panC"
                     /locus_tag="ACSIGJ_000814"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HHQ1329735.1"
                     /translation="MQIIHTIRELRTWRENIGKVAFVPTMGNLHEGHLALVREARKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLAAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPHLQNELCGKFRPGHFRGVATVVSKLFNIVLPDVACFGKKDYQQLAVIKGLTED
                     LNFDIEIVPVDTGRAADGLALSSRNRYLSVGERAEAPRLYRELQAVAESLKQGGLDYA
                     GLERQAADHLTAAGWLVDYVEIRRADTLEMARAGDKKLVVLAAARLGTTRLIDNVEVG
                     LP"
     gene            5277..7115
                     /locus_tag="ACSIGJ_000815"
     CDS             5277..7115
                     /locus_tag="ACSIGJ_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HHQ1329736.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADVELPKEVGKVLRKHRR
                     YSEEEIKNERARLAAVGERVNRVFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGEAQKRAGWLRNVLREGGNQHLDGLEEV
                     LAQSDDVQKRRIFLLLVQAAVQQGGVAQKASKAVRRAALKYEHLPEAAVADAVFGVQG
                     REKEKAIEALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLRKPDDAYARLNVLLEHNPNANLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAAELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALIGLNNIIAKLSAAGST
                     EPLAEALAQRSIIYEQFGKRGKMIADLETALKLTPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRYGIALPEPSRKPRK"
     gene            7255..7836
                     /locus_tag="ACSIGJ_000816"
     CDS             7255..7836
                     /locus_tag="ACSIGJ_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HHQ1329737.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELGRQLVGFKLPIQYLHIWAEGRRVAGAPYRIRSDGILEQYGWTIGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            7846..8691
                     /gene="ispE"
                     /locus_tag="ACSIGJ_000817"
     CDS             7846..8691
                     /gene="ispE"
                     /locus_tag="ACSIGJ_000817"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HHQ1329738.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVGGIPQEADLSYRAASLLQKYARNLAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQWQLIDLGAALGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTYEGLTRDSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSKYGSAMMTGSGACIFAAFQARNSAYNIYRQVSGLYEAYLAEGLSKH
                     PLLSV"
     gene            8699..8774
                     /locus_tag="ACSIGJ_000818"
     tRNA            8699..8774
                     /locus_tag="ACSIGJ_000818"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8732..8734,aa:Gln,seq:ttg)
     gene            8787..8862
                     /locus_tag="ACSIGJ_000819"
     tRNA            8787..8862
                     /locus_tag="ACSIGJ_000819"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8820..8822,aa:Gln,seq:ttg)
     gene            9030..10013
                     /locus_tag="ACSIGJ_000820"
     CDS             9030..10013
                     /locus_tag="ACSIGJ_000820"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HHQ1329739.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGKTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            10080..10652
                     /locus_tag="ACSIGJ_000821"
     CDS             10080..10652
                     /locus_tag="ACSIGJ_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HHQ1329740.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTAVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(10778..11947)
                     /locus_tag="ACSIGJ_000822"
     CDS             complement(10778..11947)
                     /locus_tag="ACSIGJ_000822"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HHQ1329741.1"
                     /translation="MTAHKILPVLLPIILGVSHATAASPAPNRPTVHAAPTLQTPETL
                     TAAHIVIDLQSRQTLSAKNTNTPVEPAALTQLMTAYLVFKNMKSGNIQSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALCANDAALTLADRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLGREGQVSTAKDLSLLSEALMRDFPEYYPLFSIKSFKFENIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNRALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGHTIAEKEIVALENVEKRSRWQRLWTRLTGQ"
     gene            12096..13622
                     /gene="ilvA"
                     /locus_tag="ACSIGJ_000823"
     CDS             12096..13622
                     /gene="ilvA"
                     /locus_tag="ACSIGJ_000823"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HHQ1329742.1"
                     /translation="MNTPLPYSDYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNGTFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTNNEIAKIHIRYMVGGRTDKVGHERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(13678..14754)
                     /locus_tag="ACSIGJ_000824"
     CDS             complement(13678..14754)
                     /locus_tag="ACSIGJ_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HHQ1329743.1"
                     /translation="MSITIQNLNKKRFGNFHALKNINLNVPAGKLVSLLGPSGCGKTT
                     LLRIIAGLENADGGQILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTV
                     LPKPERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLL
                     LDEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQ
                     TGSAEAIYRKPENAFVTEFLGEADAFEGRIEKGIWHYNGFAWKLDAQYKWQEQTATGY
                     IRPHEWQIAAEHETPMIRAEIEKIHAVGALTHVLVKHGKQDVHITLAGSDAARYPIAE
                     GKKLNLIPKQVYVFSQNELIEYSI"
     gene            complement(14777..14911)
                     /locus_tag="ACSIGJ_000825"
                     /pseudo
     CDS             complement(14777..14911)
                     /locus_tag="ACSIGJ_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(14895..15716)
                     /gene="cysW"
                     /locus_tag="ACSIGJ_000826"
     CDS             complement(14895..15716)
                     /gene="cysW"
                     /locus_tag="ACSIGJ_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902358 - sulfate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HHQ1329744.1"
                     /translation="MKPYSANPNLTEPRRLRMLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLSDPEAWSAVKLTLITALIAVPVNAVLGVAMAWLLTRFDFLGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGSRLEAQGIQIIFAIPGIVLTALFVTFPFAA
                     REIIPLMQTQGDSEEQAALVLGASGWQMFWRVTLPNIKWTLLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTIPLLVEIFYNEYNFTGAFSLSGVLALLALATLAVQNIITKL"
     gene            complement(15905..16741)
                     /gene="cysT"
                     /locus_tag="ACSIGJ_000827"
     CDS             complement(15905..16741)
                     /gene="cysT"
                     /locus_tag="ACSIGJ_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902358 - sulfate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HHQ1329745.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     DGFRNTIAEPNVLAAVRLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALAALYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGSIPMISEILPLIITGKLEQFDAQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            16922..17254
                     /locus_tag="ACSIGJ_000828"
     CDS             16922..17254
                     /locus_tag="ACSIGJ_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329746.1"
                     /translation="MQILSFQADIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCAMVERGEFDEVEEFDGDDDAWLEAAKDCGHH
                     GDACACGH"
     gene            17585..18094
                     /locus_tag="ACSIGJ_000829"
     CDS             17585..18094
                     /locus_tag="ACSIGJ_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HHQ1329747.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTV
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
     gene            complement(18326..18907)
                     /locus_tag="ACSIGJ_000830"
     CDS             complement(18326..18907)
                     /locus_tag="ACSIGJ_000830"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HHQ1329748.1"
                     /translation="MEHKLPQLPYEPDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPAGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPVGGLDLISTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLEGFWEIVNWDEVAKRFAA"
     gene            19071..20477
                     /gene="dnaB"
                     /locus_tag="ACSIGJ_000831"
     CDS             19071..20477
                     /gene="dnaB"
                     /locus_tag="ACSIGJ_000831"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HHQ1329749.1"
                     /translation="MNDYAAMPPEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINEGRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGRLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEYVAIEKHLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPMFIDETPNLTALELRARARRLARRFDGRLGLI
                     VIDYLQLMAASGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVEQRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQESQMKGYAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
     gene            20785..21447
                     /locus_tag="ACSIGJ_000832"
     CDS             20785..21447
                     /locus_tag="ACSIGJ_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HHQ1329750.1"
                     /translation="MCTRKQQGFTLIELLIVMAIAAIMATIALPNMGGWIASRRIASH
                     AEQVANLLRFSRGEAVRLNLPVYICPVQVKKDGAPNNGCDFSKKGRGMLAFGDKNGNK
                     AYDGDTKDVFLRNVVLNDTDDSRINYAFNHIAFGSSQPKADRVVWTFNQNGTFGYLPD
                     QNLKDNSKFVYSDGYIQIVLTDARAVSDADKKFRSAVVLIDSSGRVEVCRKNDTRAVC
                     KH"
     gene            21479..22087
                     /gene="pilV"
                     /locus_tag="ACSIGJ_000833"
     CDS             21479..22087
                     /gene="pilV"
                     /locus_tag="ACSIGJ_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HHQ1329751.1"
                     /translation="MKNNDCLRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYTGSYPPTSSDGDFKLNNL
                     ISKTDLAKAQLDRFGYELKQALPDAVAIRYAVCKDSSGDAPTLSGNTFSSNCDKKANG
                     DTLIKVLWVNDSAGDSDIFRTNLGVSGGNIVYTYQARVGGRE"
     gene            22084..23016
                     /locus_tag="ACSIGJ_000834"
     CDS             22084..23016
                     /locus_tag="ACSIGJ_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HHQ1329752.1"
                     /translation="MKRKMLNVPKGGYDGMKGFTIVEFLVAGLLSVIVLIAVVSSYFT
                     SRKLNDAANERLAEQQDLRNAATLIVRDARMAGSFGCFNMSEHIGNDVVFNVAQKNAL
                     FSLKRNSTNKLIPITESPNINYQNFFQVSSALIFQYGIDDVDASADTTVVSSCAVISK
                     PGKQIPTLEDAKKELKIQNSDKEQNGNIARQRHVVNAYAVGKIAGEEGLFRFQLNEKG
                     EWGNPQLLAKKIKRMRVRYIYVSGCPEDEDAGKEEQFKYTDKFDSSVTPAGVEVLLDS
                     GSDAKIAASSDNIIYAYRINATIRGGNVCANRTL"
     gene            22995..23606
                     /locus_tag="ACSIGJ_000835"
     CDS             22995..23606
                     /locus_tag="ACSIGJ_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX family protein"
                     /protein_id="HHQ1329753.1"
                     /translation="MRKQNTLTGIPTSDGQRGSALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGEFQVLDLEYAADSKVTFSENCEKGLCTAVNVRT
                     NDNGNEEAFGNIVVQGKPTVEAVKRSCPAKSGKNSTGLCIDNQGVEYKKGTGKVSKMP
                     RYIIEYLGVKNGQNVYRVTAKAWGKNANTVVVLQSYVGNNDEQ"
     gene            23608..24081
                     /locus_tag="ACSIGJ_000836"
     CDS             23608..24081
                     /locus_tag="ACSIGJ_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX family type IV pilin"
                     /protein_id="HHQ1329754.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNVLKQFILKNPQDNNQIIKSKLETFVLGYKMNPKIAKKYSVSVAFANTEKPRA
                     YRLVGVPKAGTGYTLSVWMNSVGDGYKCRNATSAQTYSETLSANTGCEAFSNRKK"
     gene            complement(24151..24459)
                     /locus_tag="ACSIGJ_000837"
     CDS             complement(24151..24459)
                     /locus_tag="ACSIGJ_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD family protein"
                     /protein_id="HHQ1329755.1"
                     /translation="MKDLLSFPSFLLFSCMLAVTCPTQLIGFFALRNRTLSRRAQTVM
                     ETAPGCVLISVIAPYFVSDKPHELIAIALTAFAACRFSMLFTVLIGVGSSGISGWLMA
                     "
     gene            complement(24456..25167)
                     /locus_tag="ACSIGJ_000838"
                     /pseudo
     CDS             complement(24456..25167)
                     /locus_tag="ACSIGJ_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            25333..25785
                     /gene="dut"
                     /locus_tag="ACSIGJ_000839"
     CDS             25333..25785
                     /gene="dut"
                     /locus_tag="ACSIGJ_000839"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HHQ1329756.1"
                     /translation="MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYTAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     GSEPFTVKPFERIAQMVIVPVVQAGFKRVEEFVGSSRGEGGFGSTGSH"
     gene            25857..27044
                     /gene="dapC"
                     /locus_tag="ACSIGJ_000840"
     CDS             25857..27044
                     /gene="dapC"
                     /locus_tag="ACSIGJ_000840"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HHQ1329757.1"
                     /translation="MNTLLKQLKPYPFARLREAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLRELEKYPLTAGLPELRQACANWLKRRYDGLTVNPDNEILPVLGSREA
                     LFSFVQTVLNPVSDGLKPVIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWERTKLVFVCSPNNPSGSVPDLDGWREVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQSGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWNDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVC
                     LYR"
     gene            27200..27979
                     /gene="yaaA"
                     /locus_tag="ACSIGJ_000841"
     CDS             27200..27979
                     /gene="yaaA"
                     /locus_tag="ACSIGJ_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HHQ1329758.1"
                     /translation="MFFVLSPAKNLNEKDPCPVSEFTQPDLLAESEILMRQLRELAPQ
                     QIAELMHVSDKIALLNAERNAAWHTPFTPENAKQAVFMFNGDVYEGMDANTLNTNQIQ
                     YLQNHVRLLSGLYGLMRPLDLMQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHNITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            28062..28152
                     /locus_tag="ACSIGJ_000842"
     tRNA            28062..28152
                     /locus_tag="ACSIGJ_000842"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28096..28098,aa:Ser,seq:tga)
     gene            28229..28318
                     /locus_tag="ACSIGJ_000843"
     tRNA            28229..28318
                     /locus_tag="ACSIGJ_000843"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28263..28265,aa:Ser,seq:cga)
     gene            28509..29709
                     /locus_tag="ACSIGJ_000844"
                     /pseudo
     CDS             28509..29709
                     /locus_tag="ACSIGJ_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(30065..30334)
                     /locus_tag="ACSIGJ_000845"
     CDS             complement(30065..30334)
                     /locus_tag="ACSIGJ_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329759.1"
                     /translation="MESSDVQGRMETLAFATAPKKGGIRKSGFGRVILIKSGHIVIHN
                     ADGDSFATGYGVIAADIVSPDLKTDFDIFFPPESLLRYKKIGKKK"
     gene            complement(30529..31212)
                     /locus_tag="ACSIGJ_000846"
     CDS             complement(30529..31212)
                     /locus_tag="ACSIGJ_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HHQ1329760.1"
                     /translation="MDKEKVLDKIKKCLALGRSANEHEAAQALRQAQALMEKYKVNAE
                     DIALSKVSEQKADRKMAFKLAGWQWGVANMIADIFGCKSYQRGKTMMFYGIGNRAETS
                     AYAFDVVYRQISADRRKFLKTCRAGKPSHRTYLADRFCGGWIASAWETVKKFEMSDEE
                     KAIMDGYKKKEYPDMAEARTRDAKSSILQGSKMEYEALTRGMESGKQVKLHYAVNGTG
                     FVKQIGGQT"
     gene            31337..>31524
                     /locus_tag="ACSIGJ_000847"
     CDS             31337..>31524
                     /locus_tag="ACSIGJ_000847"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329761.1"
                     /translation="MFPPAEITGRGRVVLQQGFAGFGLYFHGVKFRRLNIPFKLPASC
                     RAVKYARMFAAVSADFGG"
BASE COUNT         7554 a         7333 c         8705 g         7932 t
ORIGIN      
        1 ggcaagttcg gaacggctgt tttcgctgtt caggtaggta agggaagttt ccagtttcca
       61 tttgcccttg tcggtcatta tgtcttcaat cgtcaagggc aggtcggcat aagcggataa
      121 aggcaggatg gcgggcaagg cgggcaaaaa gatacgtttc atatttcctc ttattgatat
      181 taattcttat ttaataatag taatgaatat ccgataattc tgtttaaaca aatttttaaa
      241 tcaaaaaaaa acgatatatt tgtttttatc aattatgttt tgatatgccg tctgaaaagt
      301 ttgtgaaaaa cggttacaat ccgccgcatg aaaaaacgca ataatcctct tccgctgttg
      361 aacggtgtca aacccagtta tttggtgctg ccgcatgaaa agcagtttta cgggctgccg
      421 ctgctgcatt ttctgtgcat ccgctttcct tttttgggag cggacgattg gcggaggcgg
      481 ttgaacagcg gttttgtggt cggttcggat ggtgcggcgt tggacgaaca ttctttgttc
      541 gagcccggta agacgatgtt ttattaccgt gaaaccggtt gtgagagcga gccgcgtatt
      601 ccgtttgaag aaaagatttt gtttgtcgat gaacatttga ttgtggtgga caagccgcat
      661 tttctgcccg tcatccccgg cgggcgtttt ttgcgggaaa cgctgctcac gcgtctgcgc
      721 ctgcgtcccg aattgcagca tttgaatgtt gaagacatta cgccgattca ccgcttggac
      781 aaggatacgg cgggtgtgat gctgctgtcg cacaatcctg ccacacgcgg ggcttatcag
      841 acgatgtttc aaaacaaaac ggtatggaaa acgtatgagg cgcttgcgcc gacaaagacg
      901 gatttgccgt atccgctcga tgtggtttcg cgtttggtaa ggggtgagaa attttttacg
      961 acgcaagagg ccgaaggcgt gccgaatgcg cacacgacgg tcgaattgat tgaaaacagg
     1021 ggggaattca gcctttaccg ccttacgccg cataccggca agaaacacca gttacgcgtg
     1081 catatgatgg gtttgggtat gccgctgttg aatgacgcgc tctatcccgt gccgtctgaa
     1141 gcgggtagcg aggattatcg gaaacctttg aagcttttgg caaaaaagat tgcgtttgca
     1201 gatcctttgt cgggtcggga aagggtgttt tgcagcgggt tttgcctgta atgagaggga
     1261 acgcaaaccc gtatcggcag atgccgccgg atacgggttt gatattatcg atgctgtctg
     1321 aaaggggctt tatccgttca ggcatacggg tgcgaagcgg aggatggtgt cgcgcaggac
     1381 gatgagtttg ccgtgaaaga agtgcgtcga accggcgatg gtaatgacgg gcaaatcttg
     1441 cggttctgcc catttcaggg ctttttcgat ttcgacgacc tcatcttccg cgccgtgtat
     1501 catcagcgtt ttggcaacgt tgggaacggc ggacggctcg gggcggtcgg tatagtggca
     1561 gactgccgca ccgatgagca gcaataaatc gggtatgcgc acttgtgcgg caaatgtggc
     1621 gacatagccg ccgaaggaaa agccggataa ggcaaattcc ggggcttcgg gatgttgggc
     1681 gcgggcatag tcgatgacgg cgaggcagtc ttgcgtttcg ccgcgtccgt aatcatgtgt
     1741 gccttcgctg ccgcctacgc cgcgaaggtt gggaaggtag cagtggaagc cgagcttgct
     1801 caatgctttg gcggcggttt ggatgacttt gttggtgttc gtcccgcctt ggagggggtt
     1861 ggggtggttg atgacggcaa caccgcgtgc cggtacttgt tcggacggga tatggatggt
     1921 ttctaaaatg ccggcagggc cgggtatgtg tatggtttcg ggtttcagca taatggtttc
     1981 ctcttgattt tccgcatatt ccgatgcagc cgcgaagatg gcggcttaaa ggtggaaatg
     2041 cttgccgttg ttggggttgg acgctttcag gccggcgagc atgcggtgga agtccaatcc
     2101 gtatgcgttg ctcagtttgt cggcacgttt tttgagtttg tctatgtttt gctctttggg
     2161 gctgctttgg aaggccatta ccgcgacatt gccgtggctt tcggcaggaa gtttcaatac
     2221 gcgcccttca aaaacgctca acaaccgttc gatgaagcgt tgatagcgtt tgtcgccgct
     2281 ccaccagttg gtaacgaata tgccgtctga agagagtgcg ttgcggcagt ctctgaaaaa
     2341 cggttcttca accagcgtat cgatgatttg ttcgccgtcg aatccgtcca ccaagatgat
     2401 atcggtgttg tggcggaaga ctttgatgta ttccgcaccg tctgcttcga taatttcaaa
     2461 ttttccgccc tcgaaaggca actcaaacag gttgcgggca atggcgatga cctgaggatt
     2521 gatgtccacg gcggtttggc gcgtgtcggg caggtaggtg tctgtccagc gtgcaaacga
     2581 accgccgccc aagccgattt gggtgatatg ttgcggcagg cgttccgcaa acagcagcca
     2641 gcccatcatc gcgcggctgt aagagagcac cagctcggac gggcggtcga ggttcatcga
     2701 gctttgaacg gtgtcgctgc ccaagtgaag cgaacggata ttgccttctt cggaaatacc
     2761 gacttcggga aagccggatt ttgcaggacg caggcggcgg tagggatgtc ttgccatcgg
     2821 tgtgaacggt cggtttgaaa ggcggatatt ttatcagaat gccgatgctt gttctgtttc
     2881 aaattaattt tgttttaaat aagatactta gtgtattttt atgagggggt cgaacaaatt
     2941 tcttgccaaa gtgtgaagtt gcctgcataa ttcgccttct ttgccggtat agctcagttg
     3001 gtagagcacc tgacttgtaa tcagggggtc ccgagttcga ctcttggtgc cggcaccaga
     3061 tttgacagcc cgattgtgta aaacagtcgg gctgttttgc atttgcggca cggtcggtgg
     3121 cagggctgcg ggcatttcac tataatagcc cgtttcaaac tgaaaaggcc gtctgaaaag
     3181 ggcggggtaa caatatctga tgattactgt gaacacactg caaaaaatga aggcggccgg
     3241 cgagaaaatc gttatgctga ccgcttacga atccagtttt gccgcgctga tggacgatgc
     3301 cggcgtggat gtgctgttgg tcggggattc tttgggaatg gcggttcagg ggcgacagtc
     3361 gacgctgccg gtcagcctgc gcgatatgtg ctatcacacc gaatgtgtag cacgcggtgc
     3421 aaaaaatgcg atgattgtca gcgatttgcc gtttggtgca tatcagcaga gtaaggagca
     3481 ggcgtttgcc gccgccgccg aactgatggc tgccggcgcg catatggtca aactcgaagg
     3541 cggcgtgtgg atggcggaaa cgactgaatt tctgcaaatg cgcggaattc cggtctgtgc
     3601 ccacatcggt ctgaccccgc aatccgtgtt tgccttcggc ggatataaag ttcaggggcg
     3661 cggcggcaag gcgcaggcgt tgcttaacga tgccaaggcg catgatgaag ccggagctgc
     3721 cgtcgtactg atggaatgcg tgccggcgga actggcaaaa aaggtaactg aaactgtttc
     3781 ctgtccgacc atcggcatcg gggcgggcgc ggattgcgac ggtcaggttt tggtgatgca
     3841 cgatatgctc ggtattttcc cgggcaaaac ggcgaaattc gtcaaaaact ttatgcgcgg
     3901 gcaaagcagc atccaggctg cggttcgggc gtatgttgcc gaagtcaaag ccaaaacctt
     3961 ccctgctgcg gaacatattt ttgcagattg aacaaggtct gctgccgatg ccgtctgaaa
     4021 gccgtttcag acggcatttt gttttgccgt gcgcggcgcg tataatcggc gcgttttgtc
     4081 gggcaggaag cccgaaggat aaggattacc gtaatgcaaa tcatacatac cattcgagaa
     4141 ctgcgcacgt ggcgtgaaaa tataggaaag gtggcatttg tgccgaccat gggcaatctg
     4201 catgaagggc atcttgccct tgttcgcgaa gccagaaaac gcgccgacaa cgttgttgtc
     4261 agcatattcg tcaaccgctt gcaattcggt cagggcgagg atttcgacaa atatccgcgt
     4321 actttgcaac aggatgcgga caaacttgcc gccgaaggcg ttgccgttgt tttcgcgccc
     4381 gatgagaaag aactctatcc gaacgtggaa cagcgttaca acgtcgaacc gccccatttg
     4441 caaaacgaat tgtgcggcaa attccgcccc ggccattttc gcggcgtggc gacggtggtg
     4501 tccaagctgt tcaatatcgt tttgccggat gttgcctgtt tcggcaaaaa agattaccag
     4561 cagcttgccg tgattaaagg tttgaccgag gatttgaatt tcgatatcga aattgtcccc
     4621 gtcgatacgg ggcgggcggc ggacggtttg gcactctcca gccgcaaccg gtacttgagc
     4681 gttggggaac gcgccgaagc accgcgcctg taccgcgagc tgcaggctgt tgccgaatcg
     4741 ctgaagcagg gcggtttgga ttatgcgggt ttggagcggc aggctgccga ccatttgacc
     4801 gctgcgggct ggctggtcga ttatgtggaa atccgccgcg ccgatacgct cgaaatggcg
     4861 cgggcgggag ataagaaact ggtggtcttg gccgccgccc gtctggggac gacgcgtctg
     4921 attgacaatg tggaagtcgg cctgccttag cccgccggag ccgggaatgc cgcctgaagc
     4981 ggatttgcgt ttcagacggc attttgccgt cggctgtgct tcggaataat gtcggaaaat
     5041 agacggaatg aaataaattt ctttttgttc aacaaattaa actgtcaaac caaataataa
     5101 ggcaaggatt gtccggcatc gcatacaggc aaaccgacgc aatctcaaaa cacagccctg
     5161 ccttcgggcg ggttttgcaa atctacaaac cgtttggctt gtgataaagt gacgccttgt
     5221 ctagatactc aatatgccgt ctgaaggttc ggacggatgt cggataaagg atgattatgt
     5281 tacccgcccg tttcactatt ttatctgtcc tcgcagcagc cctgcttgcc ggacaggcgt
     5341 atgctgccgg cgcggcggat gtggagctgc cgaaggaagt cggaaaggtt ttaaggaaac
     5401 atcggcgtta cagcgaggaa gaaatcaaaa acgaacgcgc acggcttgcg gcagtgggcg
     5461 aacgggtcaa cagggtgttt acgctgttgg gcggtgaaac ggctttgcag aaagggcagg
     5521 cgggaacggc tctggcaacc tatatgctga tgttggaacg cacaaaatcc cccgaagtcg
     5581 ccgaacgcgc cttggaaatg gccgtgtcgc tgaacgcgtt tgaacaggcg gaaatgattt
     5641 atcagaaatg gcggcagatc gagcctatac cgggtgaggc gcaaaaacgg gcggggtggc
     5701 tgcggaacgt attgagggaa gggggaaatc agcatctgga cgggttggaa gaggtgctgg
     5761 cgcaatcgga cgatgtgcaa aaacgcagga tatttttgct gctggtgcaa gccgccgtgc
     5821 agcagggtgg ggtggctcaa aaagcatcga aagcggttcg ccgtgcggcg ttgaaatatg
     5881 aacatctgcc cgaagcggcg gttgccgatg cggtgttcgg cgtacaggga cgcgaaaagg
     5941 aaaaggcaat cgaagctttg cagcgtttgg cgaagctcga tacggaaata ttgcccccca
     6001 ctttaatgac gttgcgtctg actgcacgca aatatcccga aatactcgac ggctttttcg
     6061 agcagacaga cacccaaaac ctttcggccg tctggcagga aatggaaatt atgaatctgg
     6121 tttccctgcg taagccggat gatgcctatg cgcgtttgaa cgtgctgttg gaacacaacc
     6181 cgaatgcaaa cctgtatatt caggcggcga tattggcggc aaaccgaaaa gaaggtgcgt
     6241 ccgttatcga cggctacgcc gaaaaggcat acggcagggg gacgggggaa cagcggggca
     6301 gggcggcaat gacggcggcg atgatatatg ccgaccgcag ggattacgcc aaagtcaggc
     6361 agtggttgaa aaaagtgtcc gcgccggaat acctgttcga caaaggcgtg ctggcggctg
     6421 cggcggctgc cgaattggac ggaggccggg cggctttgcg gcagatcggc agggtgcgga
     6481 aacttcccga acagcagggg cggtatttta cggcagacaa tttgtccaaa atacagatgc
     6541 tcgccctgtc gaagctgccc gacaaacggg aagccctgat cgggctgaac aacatcatcg
     6601 ccaaactttc ggcggcggga agcacggaac ctttggcgga agcattggca cagcgttcca
     6661 ttatttacga acagttcggc aaacggggaa aaatgattgc cgaccttgaa accgcgctca
     6721 aacttacgcc cgataatgca caaattatga ataatctggg ctacagcctg ctttccgatt
     6781 ccaaacgttt ggacgagggt ttcgccctgc ttcagacggc ataccaaatc aacccggacg
     6841 ataccgccgt taacgacagc ataggctggg cgtattacct gaaaggcgac gcggaaagcg
     6901 cgctgccgta tctgcggtat tcgtttgaaa acgaccccga gcccgaagtt gccgcccatt
     6961 tgggcgaagt gttgtgggca ttgggcgaac gcgatcaggc ggttgacgta tggacgcagg
     7021 cggcacacct tacgggagac aagaaaatat ggcgggagac gctcaaacgc tacggaatcg
     7081 ccttgcccga gccttcccga aaaccccgga aataatgcag gcccatcctt tcagacggca
     7141 taaggtttgc cgggaagccg gggcattcgg gcaaacggca cgcagttcgc acgcgttttg
     7201 cacggcacgc cgcacccgtc ggccggcagg atgacacccg ttaaggaaac tctgatgaaa
     7261 cacaccgtat ccgcatcggt catcctgctt ttgaccgctt gcgcgcaatt acctcaaaat
     7321 aacgaaaacc tgtggcagcc gtccgaacac atcagcagtt ttgcagcgga agggcggttg
     7381 gcagtcaaag cggaagggaa aggttcgtat gcaaatttcg attggacata ccaaccgccc
     7441 gtggaaacca tcaatatcaa cacccctttg ggcagtacgc tcggacagtt gtgtcaagac
     7501 agggacggcg cattggcagt ggacggcaaa ggaaatgtct atcaggcaga aagtgcggaa
     7561 gagttgggca ggcagctggt cggtttcaaa ctgccaatcc aatatctgca tatctgggcg
     7621 gaaggcaggc gtgtggcggg cgcgccttac cgcatccgtt cagacggcat attggaacaa
     7681 tacggttgga caatcggcag aaccgccgac agtggggggc aagtccgaac gttgcaactg
     7741 aataacggaa atttgaacat caggctggtt ttcaccgaaa ttggaatgcc gtctgaaacc
     7801 gaaaccccgg aacgctgtgc ggcgcgcacg agataaggcg gacagatgaa tattgcggac
     7861 ggacggcagg cgtttcccgc acctgcaaaa ctgaatctcg atttgaggat taccggcagg
     7921 cgggaagacg gttatcacaa tatcgaaagc atattctgcc tgatagattt gcaggatacc
     7981 gtatatttga aaccgaggga cgacggcaaa atcatcctgc acaatcctgt cggcggaata
     8041 ccgcaggaag ccgatttgag ctaccgtgcc gcatcgttgc tgcaaaaata tgcgcgcaac
     8101 cttgccggcg tggaaatatg gctggacaaa aaaatcccga ccggcgccgg cttgggaggg
     8161 gggagttccg atgccgcaac ggttttgctg gtgttgaacc gttggtggca gtgtggtttg
     8221 acgcagtggc agctcattga tttgggtgcg gctttgggtg cggacgtacc gttttttatt
     8281 ttcggaaaaa atgcttttgc aagcgggata ggtgaaaaac tgataggaat ggatattccg
     8341 aaacagtggt atgttattgt caaacctccc gtccacgttt ccaccgcaaa aattttcaca
     8401 tacgaaggct tgacacggga ttctgcctca agcataatgc cgactttcca aaacctgcaa
     8461 ccgttcagaa acgatatgca ggcagtggta tttaaagaat accctgaagt ttggaaagct
     8521 tattccgagt tgtcgaaata cggttctgca atgatgaccg gctcaggagc ctgtatattt
     8581 gcagcatttc aagctaggaa tagcgcatac aatatatacc gacaagtttc aggtttgtac
     8641 gaggcatatt tggcagaggg tctttcaaaa catcctttgt tgtccgtata aacattgttg
     8701 gggagtcgtc aagcggttaa gacactggat tttgattcca gcatgcgaag gttcgaatcc
     8761 ttcctcccca gccaagccaa acaagttggg gagtcgtcaa gcggttaaga cactggattt
     8821 tgattccagc atgcgaaggt tcgaatcctt cctccccagc cagacaaaag cgtgtaagcc
     8881 tgcttacacg ctttttattt catagaaata aaaatattga aacgcctttg tttgtgtcgg
     8941 atgttgcagg tataatgtcg ggcttggtac aagcggaggg aagcattgtg ttttctgagc
     9001 ggaagttaaa cataaaatca ggtgagaata tggctgcgta cgacagtttg atggtattta
     9061 caggcaatgc caatcccgaa ttggcacaac gtgttgtcag gcatttggac atttctttgg
     9121 gcaatgcttc cgtatccaag ttttcagacg gcgaagttgc cgtcgaactg ttggaaaacg
     9181 tacgcgggcg cgacgttttc atccttcagc cgacctgtgc gccgaccaat gacaacctga
     9241 tggaaatcct gacgatggcg gatgcactga agcgtgcttc ggcaggtcgt attaccacag
     9301 ccattccgta tttcggctat gcgcgccaag accgccgtcc gcgttccgtc cgcgttccga
     9361 tttctgccaa actggtggca aatatgctgt attcggcagg gatcgaccgt gttttgactg
     9421 tcgatttgca tgccgaccag attcaaggtt tcttcgatat tccggtggac aatatttatg
     9481 ccaccccgat tttgttgaac gacatcaagc agcagcggat tgaaaatctg accgtcgtca
     9541 gcccggacat cggcggtgtc gtccgcgccc gcgccgttgc aaaatccctg aatgccgact
     9601 tggcaatcat cgacaaacgc cgcccgaaag ccaatgtggc ggaagtcatg aacatcatcg
     9661 gcgatattca aggcaaaacc tgtctgattg tggacgatat gattgacact gcaaatacgc
     9721 tgtgcaaagc cgccgtcgcc ttgaaagagc gcggtgcaga acgtgtcttg gcttatgcca
     9781 gccacgctgt attctccgga gaggcggtca gccgtattgc ctcatccgaa atcgaccagg
     9841 tggtcgtaac cgataccatt cctttgtctg aagcggctaa aaactgcgac cgtatccgtc
     9901 aggtaacgat tgcaggtttg ttggccgaaa ccgtccgccg cattagcaat gaagaatccg
     9961 tctcatatct tttcaatgaa gaagtgatga caggcagcat gttgctgccg taaccccgat
    10021 gccgtcttaa gctggtcgcg gccgatgacg gcgattttac ctaacttgga gtatttaaca
    10081 tgacttatga aattcaagcc tctgttcgtg aagcacaagg cactggtgcg agccgccgcc
    10141 tgcgtcgcga aggccaaatc cccggtattc tgtacggtga aggtcaagag cctgttgcca
    10201 ttgccgtaga tcacaaaacc gtattttacg cattggaaaa agaatctttc catactgcgt
    10261 tgattaaatt gtctctgaac ggcgaaacca aagacgttat cgtccgcgat ttccaaatgc
    10321 acccgttccg ccgcgaagtc caacacatcg acttccaagc tgtgaaagcc gatcaacttg
    10381 tacgcatccg cgttcccctg cacattgtta acgctgaaaa ttctcaagcc gttaaattgc
    10441 aaggcggtcg cgtatctctg ctgaacacgg ctgttgaagt ggttgcgttg cctgccaata
    10501 tccctgcttt cttggatttg gattgtgccg aagtggttgc cggcgacatt ttgcacttgt
    10561 cagacatcaa actgcctgaa ggtgtagaaa gcgtttccct gaaacgtaac gaaaatctgg
    10621 ctgttgctac cgttaccggt aaaaagcgct aacttacttc catcagcagg gcggcgcgta
    10681 tgcaatacgt accgccctgt tgttttatgc cgtctgaacc gtgtttcaga cggcatttct
    10741 ttatttgttg gaaaagcggg atatttgaaa cggcagatta ctgccctgtc agacgcgtcc
    10801 aaagcctttg ccaccggctt cttttttcta cgttttccag tgcgacgatt tctttttcgg
    10861 caatggtatg tccgttttgc ctgattttga tttttcctaa aatctgccct ttttttaccg
    10921 gggcgggaat cggctgtatg gtttccaaaa tctgttctgc cattttcgct tctttatgtg
    10981 gcagagtgat gtaggcttct ttgaggaagc ctgcgcggac ggtttttttg ctgcctccgg
    11041 aaatttggat ttgggcaacg gttttgcctt tcggatatat tttgggcgta tcgaaggcct
    11101 gcaatgcccg gttcagcagc ttgctgttgt ccgatgcgcg ggtttccgcc gattccgaac
    11161 ctagtgtgat gacaaggatg tgcctgccgt tgccggagta tgacacggca aggttgtagc
    11221 cgccgctttc tgtgtgcccg gctttcaggc cgtttacatt gttgtcccta tataaaagga
    11281 tattgcggtt gttttgttct atgttttcaa acttgaacga tttgatggaa aacagcgggt
    11341 aatattccgg aaagtcgcgc atcaatgctt cagacagcag ggagaggtct ttggcggtgg
    11401 aaacctgtcc ttctctaccc aagcctgtcg ggtttttgaa tacggtgttc ttcatgccca
    11461 agcgtcgggc ttctttgttc atttgttgca caaaattttc aatcgagccg ttgcccagcc
    11521 ggtcggcaag ggttagggcg gcatcgtttg cgcatagggc aatcatgcct tttaagagtt
    11581 tgtcggtgct gaccgtatcg ccgggacgta caaacattct gcttccttct gaagcccatg
    11641 cggattcggg tatttttaag ttttcttcag attggatatt tcccgatttc atgtttttga
    11701 aaaccaaata tgcggtcatc agttgggtta gtgccgccgg ttcgacaggg gtattggtgt
    11761 ttttggcgga taaagtctgc ctgctttgaa ggtcgataac gatgtgtgcc gccgtgaggg
    11821 tttcgggtgt ttggagcgtg ggggcggcgt gtaccgtcgg tctgttgggc gcgggcgatg
    11881 cagccgttgc gtgagaaacg cctaagatga tgggaagaag gacgggcagg attttatgtg
    11941 ctgtcatgaa atattctaaa ttgtgtgagt gtttcagcct gccgattata cgcttagggt
    12001 gtctgatcgg gcggattttt cttgctttcg cgccgtcttg ggcgtatggt tttgggtttt
    12061 gcgattttaa taaaccgatt atcccgcatt gaattatgaa cacgcccctt ccttattccg
    12121 attacctcat ccgcatcctg acggcatctg tctatgatgt agcggtcgaa acgcctttgg
    12181 aaccggcacg cagcctttct gtacgtttga aaaacaacat ccttttgaaa cgcgaagatt
    12241 tgcagccggt tttttcgttc aaaatacgcg gcgcgtacaa caaaatgtcc aagttgccga
    12301 aagatgcgct cgcttgcggc gtgattgcgg caagcgcggg caatcacgca caaggcgtgg
    12361 cgttgtccgc acagcgtttg ggctgccgtg ccgttatcgt catgccggag actacgccga
    12421 aaattaaagt ggatgcggtt aaaagccgtg gcggcgaggt ggttttgcgg ggtgtttcat
    12481 acaacgatgc ctacgattat gcgatggagc tggcggaaca ggaagggctg acctatatcg
    12541 cgccgtttga cgatcccgat gtgattgcgg gacaggggac ggtggggatg gaaattgtca
    12601 gccagcatcc cgatccaatc cgcgccgtat tcgtaccgat agggggcggc ggtttggcgg
    12661 cgggcgtggc ggcatttatc aaacaggttc gtcccgaaat taaagttatc ggcgtacaga
    12721 ccaacgattc ctgctgtatg aagcagtcgg tcgaagcggg cgaaatcgtc catttgaaag
    12781 atgtcgggct gttttcagac ggtacggcgg tcaaagtcgt cggaaacgga accttccgcc
    12841 tctgcaaaga acttttggat gaaatcatta cagtcgatac cgatgcggtt tgcggcgcgg
    12901 tcaaggatat tttcgatgac acgcgcagca ttaccgagcc ggccggggcg ttggcgttgg
    12961 cgggtctgaa agcctatatt gcccgagaag gtgcggaaaa ccaaaccctg attgccgtta
    13021 ccagcggtgc gaatatgaat tttcaccgtt tgcgccacgt ttcggaacgg agcgaattgg
    13081 gcgagggcaa cgaaggtatt tttgccgtta ccatccccga agaacgcggc agcttcctta
    13141 agtttgtcaa tatattggga aataggaata ttaccgagtt caactaccgc tacggcgacg
    13201 atgaaaaagc gcatatcttt gtcggacttc aagcggcagg cccgcaggat ttggcggtta
    13261 tcggcagccg gttggatgag gcgggattgc ccaatgtcga tttgaccaat aatgagattg
    13321 ccaaaatcca tatccgctat atggtcggag ggcggacgga caaagtaggg cacgagcgtc
    13381 tggtcagttt tgagtttccg gagcgtccgg gcgcgttggc gcgctttttg aaccatatgc
    13441 agggcggttg gaacattacg ctcttccatt accgcaacca cggtgcggat tacgggcgga
    13501 ttttggtcgg tatcgacgtg ccgccgcacg atgccgccgc atttgacggt ttcttggaaa
    13561 gtctgggata cagctatcac gaggaaacgc aaaatgccgc gtacaagctg tttcttgcct
    13621 gacgtttgac acgcaatgcc gtctgaaagc ctttcagacg gcattgcgct ttcagggtta
    13681 aatcgaatat tcaatcaact cgttttgaga aaaaacataa acctgtttcg gaattaagtt
    13741 taattttttg ccttcggcga ttgggtagcg cgcggcatcg ctgccagcca gtgtgatatg
    13801 cacgtcttgt ttgccgtgtt tcaccaaaac gtgcgtcaat gcgccgacgg cgtggatttt
    13861 ttcgatttcg gcacggatca tcggggtttc gtgttcggcg gcgatctgcc attcgtgcgg
    13921 gcggatatag ccggtggcgg tttgctcctg ccatttgtat tgcgcgtcca atttccacgc
    13981 gaagccgttg taatgccaga tccctttttc gatgcgtcct tcaaatgcgt cggcttcgcc
    14041 gaggaactcg gtaacgaagg cgttttcggg tttgcggtaa atggcttcgg cactgccggt
    14101 ttgttcgatt ttgccatggt tcatcacgac gatttcgtcg gaaacttcga gggcttcttc
    14161 ttggtcgtga gtcaccagaa tgctggttac gccgaggttg tgatgaatgt cgcgcagcca
    14221 ggtgcgtaat tctttgcgca ctttggcatc cagcgcgccg aagggttcgt ccaaaagcag
    14281 gagtttcggt tcgaccgcaa gcgcgcgggc gagggcgatg cgctggcgtt gcccgccgga
    14341 gagttggtgc ggataggatt tggcgaggtg ggagagttgt acgagtttga gcaattcttc
    14401 gactttggcg cggatttgtc ctttggacgg gcgttcgggc ttgggcaata cggtcaaacc
    14461 gaaagcgacg ttgtcaaaca cattcatgtg gcggaagagg gcgtagtgtt ggaacacgaa
    14521 accgactttg cgctcgcgca catgcttggc ggttacgtct tggccgtcaa agagaatttg
    14581 gccgccgtcg gcgttttcca gtccggcgat aatgcgtaaa agtgtggttt tgccgcagcc
    14641 ggagggaccg agcagggaaa cgagtttgcc ggcggggacg ttgaggttga tgtttttcag
    14701 cgcgtgaaaa ttgccgaagc gttttttgtt taagttttga atggtaatac tcatactgcg
    14761 ttcctttcgg cggcggcgag ttttttgtct tgtatagtgg attaaattta aaccagtacg
    14821 gcgttgcctc gccttgccgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat
    14881 ttaaatttaa tccactataa tttggtgatg atgttctgca ccgccagcgt cgccagtgct
    14941 aaaagtgcca atacgccgga gagggagaat gcgccggtga agttgtattc gttgtagaag
    15001 atttcgacca aaagcgggat agtgttggtt tcgccgcgta tgtgtcccga taccacgctg
    15061 accgcgccga actcgcccat cgcacgggcg ttggtgagga tgatgccgta gagcagcgtc
    15121 catttgatgt tgggcagggt aacgcgccaa aacatctgcc aaccgcttgc gccgagtacc
    15181 aatgccgcct gttcttcgct gtcgccctgt gtctgcatta aagggatgat ttcgcgtgcg
    15241 gcaaagggaa aggtaacgaa cagcgccgtc aaaacaatgc cgggaatggc gaagataatc
    15301 tgtatgcctt gcgcttccag ccggctgccc aacgccgtgt gcgcgccgaa caataagacg
    15361 aacatcaaac cggcaaccac gggcgatacg gaaaacggca aatcgagcag ggtggtcagc
    15421 aactgcttgc cgagaaagtc aaaacgggtc aacagccacg ccatcgccac gcccaagacg
    15481 gcattgacgg gaacggcaat cagcgcggta atcagcgtca atttgacggc agaccacgct
    15541 tcgggatcgc ttaaggattt caggtacaaa tcccaaccgc cttttaaggc ttcgtagaat
    15601 acggcgacga gcggcacgac cagcatcagc agcagaaagc ccagcgcggc ggcaatcagc
    15661 aacatgcgca gccggcgcgg ttcggtcagg ttgggattgg cggaataggg tttcatggcg
    15721 gtggtgttct ctggtttgtt gtttaaggtg ttcggatgcc gtctgaaagg gattgagggg
    15781 tttcagcttt cgtcggtcgg attcttgaat ccgacaaggt tgctgaatcg caaatgtttg
    15841 tcggatacaa gtatccgacc tacgaattct gtgttttggg gagcggattc gttttcagac
    15901 gacctcaacc cttcgcaccc gaacgcctgc ccaacgccca ctgcatcacg ttcagcgcaa
    15961 acagaatcac aaacgaaacc agcagcataa acaacgccac cgccgacgcg ccctgcgcgt
    16021 cgaattgttc cagcttgccc gtaataatca gcggcaggat ttcggaaatc atcggaatgc
    16081 tgcccgcgat aaaaatcacc gaaccgtatt cccccgttgc ccgcgcgaac atcatgcccg
    16141 cgccggtcaa gagtgccggc gtgatttccg gcagcagcac gcggcggaac gttgtgaaac
    16201 ggtttgcgcc caaagtcgcc gccgcttcct catattcgcc cgacaattcc tccaataccg
    16261 gctgcacggc gcggacgata aagggcaggc tgacgacaac cagcgcaatc caaatgccga
    16321 cgggcgtaaa cgcgattttg atgcccaaag gttcgaaaaa acggcctatc caaccgttgg
    16381 gcgcatacag ggccgccaac gcgatacccg taaccgccgt cggcagcgca aacggcaaat
    16441 caaccagcgc gttcgccagg cccttgcccg gaaattcata gcgcaccaac acccaagcca
    16501 ccagtgtgcc gaacacgaca ttggtcagca tcgcataaaa cgacatccgc aaactcagcc
    16561 gcaccgccgc cagcacgttc ggctcggcaa tcgtgttccg aaagccgtcc cagccgattt
    16621 ccgccgcctt cgccgccatc atcgcaaacg gcaaaaccac cagaagcgac aggcacaata
    16681 cggtcagacc gaggctgagt ttgaagccgg gcagtacgcc gggtgttttg agcggtaaca
    16741 tggtcggtaa gggtggaaaa taaaggcatt actttaacaa tgccgcctga aaaacggaaa
    16801 aaatggaaag tttggtgcaa agacgaattt gttataaagc ggttggcagt ttcttaagcg
    16861 ggcgcgatgt tctaaaataa cgctttgttt tgacggaaaa ccatcatata aaggaacact
    16921 tatgcagatt ttatcttttc aggcggacat tgcggaacgt atgctggaag gtacggaagg
    16981 cgagtcggtc aacgaaaacg cacaatttgt ccgtacggac aacggctatt ggattgcgtg
    17041 gcatgaaggc gtggcggcac tgcttgcgcc cgatacgccg ccgggcattc cctgtttttg
    17101 ggtggaaggg gcggaaagcc ttgaagagtt gtgcgcgatg gtggaacgcg gcgagtttga
    17161 cgaagtggaa gagtttgacg gcgatgacga cgcgtggctc gaagcggcta aagattgcgg
    17221 acaccacggc gacgcttgcg cctgcggaca ttaaaggcat ggcaggcttg ccgcaagggg
    17281 tgcaaggctt tgccgttttt taaataatcg gcggattgca tctgtttgca atgggcggaa
    17341 cgcttccctt tgtattttat tgaataaaaa ttatcttggt attataataa ggcagcatca
    17401 attattttgg gactgcaaca aacgcaaagc attgatttgc gtttgttgca aacttattat
    17461 tatagcaggt tgcggcgcgg acttgatggg aattgtttat agagcatgat agtctgaaac
    17521 atcattaatt aaaaatacca ttaatccgat ttatatttat ttcaatttca atggaaaaac
    17581 atcaatgaca atgattttaa gcattttaag cctgtttttt atcattagac tgttattttt
    17641 agccgtctcc attaaacatg aaaaagcctt gattgccaaa ggggcgaaac aatacggaaa
    17701 aaccaattcc acggtgcttg cggcagttca tacgctttat tatttggcgt gttttgtttg
    17761 ggtatggctt tctgacactg cttttaatgg catatccttg attggtacac tgacggtgat
    17821 ggcttcattt gtgatattat cattgattat taagcagttg ggggagattt ggacggttaa
    17881 aatctatatt ttgccaaatc atcaaattaa tcgttcgtgg ttgtttaaaa cattccgcca
    17941 cccgaattat tttttaaaca tcatacccga actgattggc atcgccttat tatgtcaagc
    18001 gtggtatgtt ttattgattg gcctgcccat ttatttgctg gtcttattta agcgtatccg
    18061 acaagaagaa caggcgatgg caacactttt ttaacccgtt tcatcaatta aaaatatatc
    18121 gacaaagaga accgtctatc aattaaatcc attcttatca ttggggcagg cttattgtca
    18181 ttgggcaagc cgtgacaaaa gcccgaacct gcaaaactta tctataaggc tgcatcagtg
    18241 cgtattgaag ccccgccttt tttgacaaaa aaatgccgtc tgaaggcttc agacggcatt
    18301 tgttcggttt ggcaaagccg gcggcttatg cggcaaaacg tttggcgact tcgtcccagt
    18361 tgacgatttc ccaaaaacct tccaggtagt tggggcggct gttgcggtag tcgatgtagt
    18421 aggcgtgttc ccacacgtcg caagtcaaga gcggcgtgtt ttcagtggtc agcggcgtag
    18481 cggcgttgga agtggaaatc aaatccaatc cgccgacagg ggttttcacc agccacgccc
    18541 aaccggagcc gaacgtaccg gccgcgcagg cattgaacgc ttcttggaat ttttcgaagc
    18601 tgccccactt cgcgtcgatg gcggcggcca gttcgccggc aggcttgcct tggcctttgg
    18661 gcgtgaaacc cagccagtag aaggtgtggt tccaagtttg agccgcgttg ttgaacacgc
    18721 cgcccgaaga ttttttcaca atctcttcca aaggcaggtt ttcaaattcg gtgcctttga
    18781 tttgattgtt caggttggtg atgtaggttt gatggtgttt gccgtagtgg aattccaaag
    18841 tctctttgct cagatgcggg gacaatgcgt ccggctcata aggcagttgc ggcagcttat
    18901 gttccatttt gtgctcctaa tgttgttttt ggatgttgtg ttttggcagt gttgctgcaa
    18961 acgaatcggc ggctcacgca ttctacctgt tttgagtgat ggaaaccaat taaacctgct
    19021 ttgcgttata atagaagatt gcaatttcgg catgacagat tggatgtacc atgaacgatt
    19081 acgcagctat gccgcctgaa gaccgtgagg tcggcgcatt gtcgctgcct ccgcattcga
    19141 tggaggcgga acaatccgtt ttgggcggtt tgatgctgga aaatccggct tgggacagga
    19201 ttgccgatgt ggtttcggga gaggatttct accgccacga acaccgcctg attttccgat
    19261 ccattgccaa attaatcaat gagggccgtc ccgcagatgt gattacggtt caggaagatt
    19321 tgcagcggaa cgaagaattg gaagcggcag gcggatttga atatctgatt acactggcgc
    19381 aaaacacccc gtctgccgca aacatccgcc gctacgccga aatcgtacgc gagcgttcca
    19441 ttatgcgcca actcgccgaa gtggggacgg aaatcgcccg cagcgcatac aatccgcaag
    19501 gcagggacgc ggggcggctt ttggatgagg cggaaaacaa agtgtttcaa atcgccgaaa
    19561 gcaccgccaa atccaagcag ggctttttgg agatgcccga tttgctgaaa gaagtcgtac
    19621 agcgcatcga tatgctttat tcgcgcgaca atcccgatga agttaccggt gtaccgacag
    19681 ggttcatcga ccttgataaa aaaacctcgg gtctgcagcc cggcgacctg attatcgtcg
    19741 ccggccgccc gtctatgggt aaaaccgcgt tctccatcaa tattgccgag tatgtcgcca
    19801 ttgaaaaaca tttgcccgtc gccgttttct cgatggaaat gggcggggcg cagttggtaa
    19861 tgcgtatgct cggttcggtc ggacgcttgg atcagagcgt cctgaaaaca ggcaggctgg
    19921 aagatgaaca ttgggggcgt ttgaacgagg cagtcgtcaa actttccgac gcgcctatgt
    19981 ttattgacga aactccgaat ctgaccgccc tcgaattgcg cgcccgcgcc cgccgcctcg
    20041 cccgccggtt tgacggcaga ttaggcttga tcgtcatcga ctacctgcaa ctgatggcgg
    20101 catccggccg ttcggacaac cgcgcttccg agcttggcga aatttcccgt tccctcaaag
    20161 ccctggcaaa agagttgcaa gtccccatca tcgccctgtc gcaattgagc cgcactgtcg
    20221 aacagcgcac cgacaaacgc ccgatgatgt ccgacctccg cgaatccggt gctatcgagc
    20281 aggatgccga cctgattatg tttatgtacc gcgacgaata ctacaaccaa gaatcgcaga
    20341 tgaagggtta tgcggaatgc atcatcggca aacaccgcaa cggtcccgtc ggtaaaatct
    20401 tcctcacatg gacgggacaa ttcaccaaat tcgacaatgc tgcctatatt cccgaggagg
    20461 caaagataga agattaaatg gctatataaa aatttattag gcggaatcag gcaaaatcgt
    20521 ttaaaatcat gcttagagat tgccctaaaa aataaaacgc ggtcttgagg catttttgca
    20581 ttcagcccgc atataattga aaatatagtg gattaacaaa aatcaggaca aggcggcggg
    20641 ccgcagacag tacaaatagt acggaaccga ttcacttggt gcttcagcac cttagagaat
    20701 cgttctcttt gagctaaggc ggggcaatgc cgtaccggtt tgaatttaat ccactataca
    20761 ttaccaatta acgtcctatt gcttatgtgt acacgaaaac aacaaggttt cacgctaata
    20821 gagctgctca tcgtgatggc cattgcagcc attatggcga cgatagccct ccccaatatg
    20881 ggcgggtgga ttgcatcacg ccgcattgcc agtcacgcgg agcaggttgc caaccttttg
    20941 cgtttttcca ggggcgaagc cgttcggctc aatctccctg tctatatctg tcctgttcaa
    21001 gttaaaaaag acggtgcgcc caacaatgga tgtgatttca gcaagaaggg gcggggaatg
    21061 ttggctttcg gcgacaaaaa cggcaataag gcatatgacg gcgatactaa ggatgttttt
    21121 ctccgcaacg tggtattgaa tgataccgac gacagccgga ttaattatgc cttcaaccat
    21181 atcgctttcg gttcgtctca gccgaaagcc gaccgtgtag tttggacgtt caaccaaaac
    21241 gggacattcg gctatttgcc cgatcagaat ctcaaggata attccaaatt tgtttattcc
    21301 gacggttata tccaaatcgt gctgacagat gcgagagcag tttcagatgc cgataagaaa
    21361 ttccgttcgg cggtggtttt gattgacagc agcggcaggg tcgaagtttg tcgtaaaaac
    21421 gatacgcgcg ccgtatgcaa acattaatga ctgttttagt cggtttggga aataaaaaat
    21481 gaagaataat gattgcttgc gcctgaaaaa tccccagtcc ggtatggcgt tgatagaagt
    21541 cttggtcgct atgctcgttc tgaccatcgg tattttggca ttgctgtccg tacagttgcg
    21601 gacagtcgct tccgtcaggg aggcggagac acaaaccatc gtcagccaaa tcacgcaaaa
    21661 cctgatggaa ggaatgttga tgaatccgac cattgattcg gacagcaaca agaaaaacta
    21721 taatctttac acggggtcgt acccccccac ttcctctgac ggcgatttca agcttaataa
    21781 tttgataagt aagacggatt tggcaaaagc ccagttggac aggttcggtt atgaattgaa
    21841 acaagccttg ccggatgcgg tagctattcg ttacgccgtc tgcaaggatt cgtcgggtga
    21901 cgcgccgaca ttgtccggca atactttttc ttcaaattgc gacaagaagg caaacgggga
    21961 tactttgatt aaagtattgt gggtaaatga ttcggcaggg gattcggata ttttccgtac
    22021 gaatcttggg gtgagcggcg gcaatatcgt atatacctat caggcaaggg tcggaggtcg
    22081 tgaatgaaac gtaaaatgct aaacgtacca aaaggcggtt atgatggtat gaagggtttt
    22141 accattgttg aatttctggt tgcgggcctg ctcagtgtga ttgtcctgat agcggtcgta
    22201 tcgagttact ttacatcccg gaaattaaat gatgcggcaa acgagcgtct tgccgagcaa
    22261 caggatttgc ggaatgcggc aacattgatt gtccgcgatg caagaatggc ggggagcttc
    22321 ggttgtttca atatgtctga gcatatcgga aatgatgttg ttttcaatgt ggcgcaaaaa
    22381 aacgctcttt tttccttaaa aaggaacagt acaaataaac ttatccccat aacggaatct
    22441 ccaaatatca attatcagaa ttttttccag gttagtagcg cattgatttt tcaatacgga
    22501 atcgatgatg ttgatgcaag cgccgatact accgtcgtca gcagctgtgc cgtaatatcg
    22561 aaaccgggta agcaaatccc tactttagaa gatgcaaaaa aagaattgaa gattcagaat
    22621 tctgataagg agcaaaatgg caatatagcg cgtcaaaggc atgtggtcaa tgcctatgcg
    22681 gtcggcaaga ttgccggtga ggaaggtttg ttccgcttcc aattgaatga aaagggcgag
    22741 tggggtaatc ctcagttgct cgcgaaaaag attaaacgta tgagagtgcg gtatatctat
    22801 gtttccggtt gtcctgaaga tgaagatgcc ggcaaagagg aacaattcaa atatacggat
    22861 aaattcgaca gctctgttac gcctgccggg gtggaggttt tattggatag cggtagtgat
    22921 gccaagattg ccgcttcttc agataatatt atttatgctt accgtatcaa tgcgacaata
    22981 cgcgggggaa atgtatgcgc aaacagaaca ctttgacagg aatcccgact tctgacggac
    23041 agagggggtc cgcactgttt atcgtgctga tggtgatgat agtcgtggcc tttttggttg
    23101 taactgccgc ccagtcctac aataccgaac agaggatcag tgccaacgaa tcagacagga
    23161 aattggcttt gtctttagcc gaggcggctt tgcgggaggg cgaatttcag gttttggatt
    23221 tggaatatgc tgcggacagt aaggttacgt ttagcgaaaa ctgtgaaaaa ggtctgtgta
    23281 ccgcagtgaa tgtgcggaca aatgataatg gtaatgaaga ggcttttggc aatatcgtgg
    23341 tgcaaggcaa gcccaccgtt gaggcggtga agcgttcttg ccctgcaaag tctggcaaaa
    23401 attctaccgg cctgtgcatt gacaatcagg gagtggaata taagaaaggt acgggaaaag
    23461 tcagcaaaat gccgcgctat attatcgaat atttaggcgt gaagaacgga caaaatgttt
    23521 atcgggttac tgccaaggct tggggtaaga atgccaatac cgtggtcgtc cttcaatctt
    23581 atgtaggcaa taatgatgag caataaaatg gaacaaaaag ggtttacatt gattgagatg
    23641 atgatagttg tcgcgatact cggcattatc agcgtcattg ccataccttc ttatcaaagt
    23701 tatattgaaa aaggctatca gtcccagctt tatacggaga tggtcggtat caacaatgtt
    23761 ctcaaacagt ttattttgaa aaatccccag gacaataatc agatcatcaa gagcaaactg
    23821 gaaacatttg tcttaggcta taagatgaat ccgaaaattg ccaaaaaata tagtgtttcg
    23881 gtggcatttg ccaatacgga aaaaccaagg gcatacaggt tggtcggcgt tccgaaggca
    23941 gggacgggtt ataccttgtc ggtatggatg aacagcgtgg gcgacggata caaatgccgt
    24001 aatgccactt ctgcccagac ctattcggag accttgtccg caaataccgg ctgtgaagct
    24061 ttctctaatc gtaaaaaata gggttgtttt gccaataccg tctgaagatc aattttcaga
    24121 cggtattttt tatgggcatt ttgaaatgaa ttatgccatc aaccatcccg agatgcctga
    24181 cgagccgacc cctatcaata cggtaaacaa catagaaaaa cggcaggccg caaaggcggt
    24241 taacgcgatg gcaataagtt cgtgcggctt gtcggagaca aaataggggg caataacgga
    24301 aatgaggacg cagcccggcg cggtttccat aacggtttgg gcgcggcggc ttaaggttcg
    24361 gttacgcaat gcgaaaaagc cgattaactg ggtagggcag gtaacggcaa gcatacagga
    24421 aaacagcagg aatgatggga acgacaataa atctttcatt tccgcccatt ccaaaaatag
    24481 gcggacaaca ggccggacag cgtgccggcg ggaacatacc acgcgccgtc aacggtcagg
    24541 taaacggcgg atgcgacaaa caggttgaca aaccaaagtc ttgatgcgga aaaacttttc
    24601 cacattcccc tcagtaaaac caaaaatacg gcaggaaacg ccataccgaa cccccaagcg
    24661 gccacattac cgaacacggg ccctactgcc gcgccgaggg cggcaaaccc tatccaggta
    24721 atgtagagga taaagcatgc gcccatataa aaaggcatat tgaatgcagg caaaccggct
    24781 gctttccgtt tttggatttc ggagaatgcc atggcccggc tttcatcaca cataaaaagc
    24841 agcgcgggca cggctttttt cagcggtatt tctttcatgt gcgtggcaag cgccgccccc
    24901 catcaggata tgccgcgaat taatcataaa ggttatggtg gcgataggca gtatcggcag
    24961 aggttccgcc cacaggttga ccgtggcaaa ttcggagccg ccggcgaagt tcatccccgt
    25021 catcagcagc atttccagcc ggcccatacc tttttgcccg ccctgcattc cgagtatcaa
    25081 tgcccaaggc aaaagcccaa tcagcatggg cgaactttct ttgatgccgc gtatgaattc
    25141 atcacgtgga gaggctgtgt gcgtcatgtt aatgtttata tatgaagaga cggggcggta
    25201 tcatacacag gcagaatgtt tttgacagtc gaggatggtt tcaaaacaaa agcagtttgt
    25261 tgtgtttcag cgtaaacacg gcttgtgtat aatctccaat ctttgaaacc ggccgtatgc
    25321 aggagcagga cgatgaatat tgaagtagaa atgaaagtgt tggacgagag gatggcggat
    25381 tttatccctg cctatgcgac ggagggttct gcaggtttgg atttgcgcgc ctgtttggat
    25441 gaggaagtcg ttttgcagcc gggcgaaacg tttcttgtgc cgacaggttt ggcaatttat
    25501 ttggcgaatc ccgcatatac cgccgttttg ctgccccgtt ccggcttggg gcataaacac
    25561 ggcattgtct tgggcaattt ggtcggctta atcgactccg attatcaggg agaattgaag
    25621 gtgtcgttat ggaatagggg cagcgaaccg tttactgtca aaccgttcga gcgtatcgca
    25681 caaatggtta tcgtgccggt cgtacaggcg ggcttcaaac gtgtcgagga atttgtcgga
    25741 agcagccggg gtgagggcgg cttcggcagt acgggttctc actaaagata tagaatgccg
    25801 tctgaaggac ggttcaggcg gcatatcttc cgatataccg ataaccggag aaaatcatga
    25861 ataccttact caagcagctc aaaccatatc cctttgcccg actgcgcgag gcgatgcagg
    25921 gcatttccgc gcctgaaggc atggaagccg tcccactgca tatcggcgaa ccgaaacacc
    25981 cgacaccgaa agtcattacg gatgcgctga ccgcctcatt gcgcgagttg gaaaaatatc
    26041 cgctgactgc gggtctgcct gaactgcgtc aggcgtgtgc gaactggtta aaacgccgtt
    26101 atgacggctt gacggtcaat ccggataatg aaattctgcc ggttttgggc agtagggagg
    26161 cgttgttttc ttttgttcaa accgtgttga accctgtttc agacggccta aaacccgtaa
    26221 tcgtcagccc gaatcccttt tatcagattt atgaaggtgc gacacttttg ggcggcggtg
    26281 aaatccattt tgccaattgc cccgcgccgt ctttcaaccc cgattggcgc agtatttccg
    26341 aagaggtttg ggaacgcacc aaactggtgt tcgtctgctc gcccaacaac cccagcggca
    26401 gcgtgccgga tttggacgga tggagagaag tttttgattt acaggataaa tatggtttca
    26461 ttattgcctc ggatgaatgc tattccgaaa tctattttga cggcaacaaa cctttgggct
    26521 gcctgcaggc cgccgcacag tcggggcgaa gcaggcaaaa actgcttatg tttaccagct
    26581 tgtccaagcg gtccaatgtt ccgggcctgc gttccggttt tgtcgccggt gatgcggaac
    26641 tgcttaagaa ctttctgctt tacagaacct atcacggcag cgcaatgagc attcccgtgc
    26701 agcgtgccag tattgccgcc tggaatgacg aacaacacgt tatcgacaac cgccgcctgt
    26761 atcaggaaaa atttgagcgt gttattccca ttttgcaaca ggtatttgac gttaaattac
    26821 cggatgcctc gttttacatc tggttgaaag tccccgatgg cgacgatttg gcattcgcac
    26881 gcaatttatg gcaaaaagcc gctatccaag tattgcccgg acgctttttg gcacgggata
    26941 ccgaacaggg caatcccggg gaaggttatg tgcggattgc tttggttgca gatgtcgcaa
    27001 cttgtgtcaa agctgcggaa accattgttt gcttatatcg gtaaagaata aaaaatgccg
    27061 tccggacttt tcctgacggc tttttttaat actatcaaga tgtatttatt ataacggtat
    27121 ttacatcaca atcaatcggc ttgcctgagg atgcttttaa atctaaaatt ccgacgtttg
    27181 acactatacc gagcggatta tgttttttgt cctttcccct gcgaaaaacc ttaacgaaaa
    27241 agacccttgc cctgtcagcg agtttaccca acccgatctt ttggcggaat ctgaaatcct
    27301 gatgcgccaa ctgagggagc ttgcccctca acaaattgca gagttgatgc acgtttccga
    27361 caaaatcgca cttctaaacg ccgaacgaaa tgcagcgtgg cacacgccgt ttacgccgga
    27421 aaacgccaaa caggcagtct ttatgttcaa cggcgatgtt tacgaaggta tggatgcaaa
    27481 cacattgaat accaatcaaa tacaatatct gcaaaaccat gtccgccttc tgtccggctt
    27541 gtacggtctt atgcgtccac tggatttgat gcagccctac cgcctggaaa tgggaacggc
    27601 atttgccaat ttgcgcggca agaatttgta tgagttttgg ggcgacatca ttaccaacct
    27661 tttaaatgac acgcttgccc aagcaggcag caatacgctt gtcaaccttg cctcacagga
    27721 atatttcaag tccgtcaaca cgaaaaaact tcgggcgcgg ctgattaccc cgatatttaa
    27781 agacgaaaaa aacggtaaat ataaaatcat cagtttctat gccaaacgcg cgcgcggatt
    27841 gatggtgcgc tatgcggcag aacacaatat taccgatcct gaaatgctga aaaattttaa
    27901 ttacgaaggc tacgcattca atgacgcggc ttccaatgaa agcgaatggg tgtttatgcg
    27961 ttcggaacaa ataaagtgaa aacaataaat taagtatttc ccaaaaaaag tgcttggcaa
    28021 aatgtataaa cttcattatt attcctactc ttcaagaaga cggaagcgtg gcagagcggt
    28081 ttaatgcaac ggtcttgaaa accgtcgagg gttgatagcc ctccgtgagt tcgaatctca
    28141 ccgcttccgc caatttttga gcgtaaaacc aaataaaaat gcaatagccg cgaatattgt
    28201 tatttttatt tggttttact gcattatcgg aaacgtggca gagaggctga atgcagcgga
    28261 ctcgaaatcc gctgagggtg caaatcctcc gtgggttcga atcccaccgt ttccgccaga
    28321 aaacataacc gccctgattc agggcggttc ttttttgttc aagttttgtt gattaccata
    28381 taaaaatcat cggcttgacc tatcataacg cttcaaggca aatcatttgc aatcttgcgg
    28441 catcttctta ttgcattttt ttatggtaat ttatatggta aattttggac aaacgggaat
    28501 ttaccataat ggcgaaaatc attacgccgc tgtcggcaaa tcaggttaaa aatgcgaagc
    28561 cgcgcgataa gctgtataag ttgtcggacg ggggggctgg ctttgtgggt ctacccgacg
    28621 ggcggacgga gttggaagct gtcgtttatg caggacggaa ggcaacagac aatttcgctg
    28681 gggcggtatc ctgatttctc gctggctgag gcgcgggaat ggcgggagga ggtgcgccga
    28741 aaacgggcac acggggaaaa tgtcgtcaat aagaaggtgc gggcggattt tgcttttgag
    28801 aaggtggcgc gtgattggtt tgtgcgttgg tcgaaggggc ggtctgaaaa gtatgccgga
    28861 caggttatgc ggaattttga acggtgggtt tttccggcta tcggcaatct tgatatccgt
    28921 caagtcagga cggcggacgt ggtcggctgc ctgcgtgtga tggaggcgcg cggtatcgtt
    28981 gatacgttgc gcaaaacgaa aaacagtctg aagatggtgt ttgcgtttgc gtttgcggtc
    29041 ggttcgggaa tgatggaaat caaccctgtc gcgcaaatcg gttcgggtgt gtttgaacgg
    29101 gtgaaaacca aaaacatgac ggcgttgagt ccgtccgaat tgccgcgcct gattgatttt
    29161 ttggagcagc gcaatgaatt tgcggtttat gcgggcaggg tgcatatcca tcctgtaacg
    29221 cggttttgta tctattggct gctgttgacg atgacgcgga ttcgggaggc ggcgttgatg
    29281 gagtggtcgg agttggacgg ggaggtttgg cgtatccccg ccgaacggaa aaaggagcgg
    29341 cgggggcatg atgtgccgct gtcgcgggcg atgcggtggg tgttggatca ggcgcggggg
    29401 ctgaatgtga acgggcggtt tgtgtttgaa agtgtgaatt ttcaagggca tatcaataag
    29461 gaaagtccgc gcgtggcgat gcggcggtcg gggctggata cgacggcgca cggtttgcgc
    29521 tcgcttgcgc gtacttattt gcgcgaggtt ctgaaggtgt ataatgatgt ggcggaaaag
    29581 ctgcttgccc attcgttggg gacgaggacg caaacggctt acaaccgctc ggagctttgg
    29641 gaggagcgta aggatgcgcc ggaacggtgg gggaatgatg ttttgaggct tgccgacaac
    29701 ggaaaatgat ttttttgtgt gttttgcgtt atgtggaaat gtagataaga aagctgtttc
    29761 cgcattatgt gaaaacgccc taatcgggcg tttttttatt gctgcaaccc taatgcttcc
    29821 aaaacttcgc gggtgtccca tacgggggat gcgagggagg ttcagacggc acggtatccg
    29881 tccgtctttt tccatacgca gcagtgtcga gtttgaaatg gggtggttgc ggcaggttgc
    29941 gtaggcaatc agttcgcgga tggtcgggcg gtctatcctt gcgcccggtt tggtgatgtt
    30001 cattttttta ctctcctgtt cggtttaatc ttgcgccgtt tttacggctc tgtcgtttgt
    30061 ctgcctattt cttcttcccg atttttttat atcgcaacaa gctttccggc gggaagaata
    30121 tgtcaaagtc tgttttcaaa tcgggactga cgatgtcggc ggcaatcacg ccgtagccgg
    30181 tggcaaagct gtcgccatca gcgttatgaa taactatatg cccgcttttg attagaataa
    30241 cgcggccgaa tccggatttc cggattccgc cttttttcgg ggcggttgca aatgccagtg
    30301 tttccatccg cccttgtacg tcggaagact ccattgcttc gccgaccgct tttttgacgg
    30361 gcgtttattg aatacttcgt attccatgtc ttcaatcctc tttccgttga ttccgatttg
    30421 tccgggtttc cgctttttta cggcttggca tgcggcaatt gcgtctttca tattcggcat
    30481 atcctttccc caatccgccg aagtttccgc atccgccgcc gtatcgattc atgtttgccc
    30541 tccgatttgt tttacgaagc ccgtaccgtt cacggcatag tgtaacttca cttgcttacc
    30601 cgattccatt cctcgggtta atgcctcata ttccattttt gacccttgta ggattgacga
    30661 tttcgcatct cttgtccttg cttcggccat atccggatat tctttctttt tgtatccgtc
    30721 cataatggct ttttcttcat ctgacatctc aaattttttg actgtttccc aagcgctcgc
    30781 aatccaaccg ccgcaaaatc ggtcggcaag ataagttctg tgcgagggct ttcccgcccg
    30841 gcaggttttc aaaaatttgc ggcggtcggc ggaaatctga cgatagacta catcaaaggc
    30901 ataggctgag gtttccgctc gatttccaat gccgtaaaac atcattgttt tcccgcgttg
    30961 gtaagatttg cacccgaata tatcggcaat catatttgca acgccccatt gccagcctgc
    31021 caatttaaaa gccattttcc gatcggcttt ctgttcggag acttttgata aggcgatatc
    31081 ctcggcattt actttgtact tttccattaa agcctgcgcc tgccttaacg cctgtgcggc
    31141 ttcatgttca tttgccgacc gacccaaagc caaacatttc ttgattttgt ctaagacttt
    31201 ctctttatcc atattaaacc ctttaatttg tccggtattt taatttcgca cccttgccca
    31261 ttcgtctttc gctttgcttt cgattcggtc gggccattcg gctagggctg atacgcttac
    31321 gaactcgggg ctttttttgt ttccgcctgc cgagattacc ggccgcggca gggtagtttt
    31381 gcaacaaggc tttgcaggct tcgggctgta tttccacggc gtgaaattca gacggcttaa
    31441 tataccgttc aagctgcctg cttcctgccg cgccgtcaaa tacgcccgga tgttcgccgc
    31501 agtatcggcg gactttggcg gcaa
//
LOCUS       DBJGGI010000019        30503 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.019, whole genome shotgun sequence.
ACCESSION   DBJGGI010000019 DBJGGI010000000
VERSION     DBJGGI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30503
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.019"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            36..248
                     /locus_tag="ACSIGJ_000848"
     CDS             36..248
                     /locus_tag="ACSIGJ_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HHQ1329762.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWIEKTNAV"
     gene            354..947
                     /locus_tag="ACSIGJ_000849"
     CDS             354..947
                     /locus_tag="ACSIGJ_000849"
                     /inference="COORDINATES: protein motif:HMM:TIGR01644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098025 - virus tail, baseplate [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HHQ1329763.1"
                     /translation="MEACRKRQKPPRSAITGRFPTSRRDRNDRPPNRQPDQTRHHRRH
                     RPRVRAQHGGITTDWLPYIVPFAGGVSVWRIPSVGEACTILSPAGEPENGVVLCCQAS
                     DRYPAPSADPAETVVRFPDGTHIRYNHNSGAMELKAVTSLTIDTPQTTITGHLTVNQT
                     TTAQGLLTYQNGMNGQGGSLSEHTHPDDSGGTTEKPQ"
     gene            944..1270
                     /locus_tag="ACSIGJ_000850"
     CDS             944..1270
                     /locus_tag="ACSIGJ_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HHQ1329764.1"
                     /translation="MTDAENGRGQDTLAHIAQSIRNILFTRIGTRLMREEYGSFIPDL
                     IDMPAGHAAIALIHQAAVTALARWKPRITVRRIQADTADLAAGKIKLTLDVTLADGGE
                     RTYRIK"
     gene            1291..2346
                     /locus_tag="ACSIGJ_000851"
     CDS             1291..2346
                     /locus_tag="ACSIGJ_000851"
                     /inference="COORDINATES: protein motif:HMM:NF016736.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098025 - virus tail, baseplate [Evidence
                     IEA];
                     GO_process: GO:0098045 - virus baseplate assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HHQ1329765.1"
                     /translation="MGNSRLSQLPAPAAIEETDFEGIFARKKAALTALCPESIRETVA
                     QTLELESEPLTIDLQQQAYQELLVRNRINEAVKANLLAYAQGSDLDHIAAQYGLSRKT
                     IRAADPDANPPVAAEYETDDAFRARVQAHPEKYAAGPRTAYEAHAIDAHPQITHARAV
                     RRAAGTVEVYIKTQSGTPDETILTAAREYLSAETRRPLCDNVQVTAAQPKDAAVEYSA
                     EYHPAANIQAERQTACEALDNLWRQNAHIGASVALSKIIGALDTPGVKKNHTAQPRRR
                     HRMRQRRIHPNHVHARARINEQHRPREQQPPATRTGKADRTRNCRRLPPTRPRIFTLS
                     RLRKKHRHGRGLGLCRN"
     gene            2231..2725
                     /locus_tag="ACSIGJ_000852"
     CDS             2231..2725
                     /locus_tag="ACSIGJ_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098025 - virus tail, baseplate [Evidence
                     IEA];
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HHQ1329766.1"
                     /translation="MTERETAAVSRQLDPGFLPFHAFARSIGTEEGWDFAETDEARRN
                     LIAGFAEIHARKGTPYAIRALFPILRLGEIQIIERDGEFKWDGSVLFDGSRTFGRRKG
                     DWAEYRIVLTRPVSIRQTARIRAMLAEIAPLRCELTALDYRNHPHRWNGKIRFNGEYG
                     FGTT"
     gene            2765..4952
                     /locus_tag="ACSIGJ_000853"
                     /pseudo
     CDS             2765..4952
                     /locus_tag="ACSIGJ_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242799.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin knob domain-containing protein"
     gene            5064..5393
                     /locus_tag="ACSIGJ_000854"
     CDS             5064..5393
                     /locus_tag="ACSIGJ_000854"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329767.1"
                     /translation="MPRQSCHSSPFPRKRESGTQTRQESIGKNNPTAVIPAQAGIQTH
                     NVKAVYQKKPKPNALDFRLRGNDEELGSDERREQPRKKPPTPSDGIGNKNRTAETARE
                     RIAGRAR"
     gene            complement(5409..5720)
                     /locus_tag="ACSIGJ_000855"
     CDS             complement(5409..5720)
                     /locus_tag="ACSIGJ_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HHQ1329768.1"
                     /translation="MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQS
                     SITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQRKTFDIAPLFPEPPLSSMKTGAR
                     T"
     gene            complement(5717..6010)
                     /locus_tag="ACSIGJ_000856"
     CDS             complement(5717..6010)
                     /locus_tag="ACSIGJ_000856"
                     /inference="COORDINATES: protein motif:HMM:NF016875.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HHQ1329769.1"
                     /translation="MMIVSFKHKGLERFFKTGSLSGIQAGHSVKLNLLLTALNAAQTP
                     SDMAVPSWNLHPLKGSLSGHWAVQSQRKLAFDFSFQRRQCRSCRLSGLSLGII"
     gene            6092..6705
                     /locus_tag="ACSIGJ_000857"
                     /pseudo
     CDS             6092..6705
                     /locus_tag="ACSIGJ_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6684..6848
                     /locus_tag="ACSIGJ_000858"
                     /pseudo
     CDS             6684..6848
                     /locus_tag="ACSIGJ_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
     gene            6952..7083
                     /locus_tag="ACSIGJ_000859"
                     /pseudo
     CDS             6952..7083
                     /locus_tag="ACSIGJ_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
     gene            complement(7167..7550)
                     /locus_tag="ACSIGJ_000860"
     CDS             complement(7167..7550)
                     /locus_tag="ACSIGJ_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HHQ1329770.1"
                     /translation="MIYYPAVLFQDPDQSNWGVIIPDLPGCYPVGDTVADALADAKAA
                     AMFHLEGLLQENLPIPEAQSIEVNRDNPDYADAVLWTMVEADDAALTGQVRFNVSWPQ
                     HILNRVDACTAARHETRAVFWRKPP"
     gene            complement(7655..7795)
                     /locus_tag="ACSIGJ_000861"
     CDS             complement(7655..7795)
                     /locus_tag="ACSIGJ_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329771.1"
                     /translation="MPQYRNWQSGLDAKCADGDEYGVAQSDCRTREINAKTKEIQGYL
                     ID"
     gene            complement(7840..8853)
                     /locus_tag="ACSIGJ_000862"
     CDS             complement(7840..8853)
                     /locus_tag="ACSIGJ_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HHQ1329772.1"
                     /translation="MKKNLPALALASMLILSGCDRLGIGNPFSGKEISCGSEETKEIL
                     VKLVRDNVEGETVKTFDDDAFKDQAFADIGISHIRRMVERLGITVDEVRTTEKTDTSS
                     KLKCEAALKLDVPDDVVDYAVAANQSIGNSYKKTPDFFEPYYRKEGAYYVKTISYSVQ
                     PTDDKSKIFAELSQAHDIIHPLSELVSMALIKEPLDKAKQRNEKLEAAEATAQEAREA
                     EEAAAQEALGREQEAARVSEWEERYKLSRSEFEQFWKGLPQTVQNKLQASQKTWKSGM
                     DKICANNAKAEGETPNGIKVSELACKTAETEARLEELHNRKKALIDEMVREEDKKELP
                     KRL"
     gene            complement(9070..10716)
                     /gene="pgi"
                     /locus_tag="ACSIGJ_000863"
     CDS             complement(9070..10716)
                     /gene="pgi"
                     /locus_tag="ACSIGJ_000863"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HHQ1329773.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIMVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     TMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNRKAVAEFGIDIANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDRHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMCGKTPDEVRAELKAQGMDEA
                     RIEELVPHKIFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETEPQKHDSSTERLINLYLQTNRK"
     gene            complement(10942..11790)
                     /gene="hexR"
                     /locus_tag="ACSIGJ_000864"
     CDS             complement(10942..11790)
                     /gene="hexR"
                     /locus_tag="ACSIGJ_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HHQ1329774.1"
                     /translation="MLSKISESLADLSGAERKVAECALAEPKWFVHAAVAEIADRASV
                     SQPTVIRFCRSLGYKGLPEFKLSLSASIGHEGMPYVHEELNADDDMANVVEKVLGNAA
                     AALLGERRFLKESELENGIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            complement(11839..12825)
                     /locus_tag="ACSIGJ_000865"
     CDS             complement(11839..12825)
                     /locus_tag="ACSIGJ_000865"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HHQ1329775.1"
                     /translation="MSSTPNKHADYPRLVADIGGTNARFALETAPCVIEKVAVLPCKE
                     YDTVTDAVRAYLNQSGATGVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQALGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSPAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYRHVSAERFLSGAGLSLIYETLAAKQKAEP
                     AKLMPSEITEKALNCESPLCRQALDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRM
                     LDYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGIAGAAAALGNHLKNV"
     gene            complement(12806..13501)
                     /gene="pgl"
                     /locus_tag="ACSIGJ_000866"
     CDS             complement(12806..13501)
                     /gene="pgl"
                     /locus_tag="ACSIGJ_000866"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HHQ1329776.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTNHADSNTGLVREYLLKNKAAAAVWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLILGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERISMTLDAIAHTGHVFLAIQGEEKKAVFDQAAQGENREYPISLVL
                     NHQGVNCHVFYAE"
     gene            complement(13653..15098)
                     /gene="zwf"
                     /locus_tag="ACSIGJ_000867"
     CDS             complement(13653..15098)
                     /gene="zwf"
                     /locus_tag="ACSIGJ_000867"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HHQ1329777.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSGEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQACENLAAIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTIE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFRPLQNHIFDNSQTAPNRLVIELQPNESVRLYTQVKTPGAGNKVEV
                     TPLGVDLGKAVEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWANNT
                     TPPHGYGAHSWGPEAARELLARDGNKWHEEQ"
     gene            15425..15534
                     /locus_tag="ACSIGJ_000868"
                     /pseudo
     CDS             15425..15534
                     /locus_tag="ACSIGJ_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            15618..17453
                     /gene="edd"
                     /locus_tag="ACSIGJ_000869"
     CDS             15618..17453
                     /gene="edd"
                     /locus_tag="ACSIGJ_000869"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HHQ1329778.1"
                     /translation="MNHTPIHPKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRDELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLDAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVVKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFICVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVTGELNVLINEAE
                     WNVREVERIDLGANQQGCGRELFAGFRSMTSSAETGAMSFGGEFA"
     gene            17662..18300
                     /locus_tag="ACSIGJ_000870"
     CDS             17662..18300
                     /locus_tag="ACSIGJ_000870"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HHQ1329779.1"
                     /translation="MSKLTPRQILTAGAVVPVMAIDDLSTAVDLSRALVEGGIPTLEI
                     TLRTPVGLEAIRLIAKEMPNAIIGAGTVTNPEQLKAVEDAGAVFAISPGLHESLARAG
                     RNSGIPLIPGVATPGEVQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(18489..18674)
                     /locus_tag="ACSIGJ_000871"
     CDS             complement(18489..18674)
                     /locus_tag="ACSIGJ_000871"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329780.1"
                     /translation="MGYFGGNGGYLKRKQPKNLWLDVGCLKKRNFAKVSERYFLTTVN
                     LLVSAAHHGILPIGRFL"
     gene            complement(18685..18797)
                     /locus_tag="ACSIGJ_000872"
                     /pseudo
     CDS             complement(18685..18797)
                     /locus_tag="ACSIGJ_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18895..19935)
                     /locus_tag="ACSIGJ_000873"
     CDS             complement(18895..19935)
                     /locus_tag="ACSIGJ_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HHQ1329781.1"
                     /translation="MKAMVYHGANDIRFEEKPRPQIIDPTDAVVKIVKTTICGTDLGI
                     WKGKNPEVADGRILGHEGIGIVEEVGEAVKNIKVGDKVIISCVSKCCTCDNCKIQLYS
                     HCRNGGWILGYMIDGTQAEYVRTPYADNSLVPPHDNVNEEIALLLSDALPTAHEIGVQ
                     YGDVKPGDTVFIAGAGPVGMSALLTAQLYSPAAIIVCDMDENRLKPAKELGATHTISP
                     ASGDVSKQVFAIVGEDGVDCAIEAVGIPATWNMCQDIVKPGGHIAVVGVHGQSVDFKL
                     EKLWIKNLAITTGLVNANTTEILMKAIFSSSVDYTKMLTHRFKFSELEKAYDVFKHAA
                     ENQAMKVVLEAD"
     gene            20098..21138
                     /gene="mutY"
                     /locus_tag="ACSIGJ_000874"
     CDS             20098..21138
                     /gene="mutY"
                     /locus_tag="ACSIGJ_000874"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HHQ1329782.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VAAVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYGRARNLHKAAQQIVGQFGG
                     TFPSERKDLETLCGVGRSTAAAISAFAFNRRETILDGNVKRVLCRVFAQDGNPQDKKF
                     ENSLWTLAESLMPSENADMPTYTQGLMDLGATVCKRTKPLCRQCPMADICEAKKQNRT
                     AELPRKKTALEVQTLPLYWLIVRNRDGAILLEKRTAKGIWGGLYCVPCFESLNGLSDF
                     AAKLSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSEHHSDGIWIKPGHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            complement(21514..21828)
                     /locus_tag="ACSIGJ_000875"
     CDS             complement(21514..21828)
                     /locus_tag="ACSIGJ_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329783.1"
                     /translation="MFGAVPDNRVKFPLAHYFKIEILYFISLLLKRCSHFFIGFIIHH
                     LIYGFWRQGLTAFAAVIDFWNRGNAFKESKAIRPDADGGAIIHLLEFVAIGADSCDDY
                     VE"
     gene            complement(21900..23642)
                     /locus_tag="ACSIGJ_000876"
                     /pseudo
     CDS             complement(21900..23642)
                     /locus_tag="ACSIGJ_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799051.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
     gene            complement(23794..23910)
                     /locus_tag="ACSIGJ_000877"
     CDS             complement(23794..23910)
                     /locus_tag="ACSIGJ_000877"
                     /inference="COORDINATES: protein motif:HMM:NF016702.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329784.1"
                     /translation="MLGTAAGAAEGNSSADAVWGCFQTASDFASSFSYPINM"
     gene            complement(24052..24498)
                     /locus_tag="ACSIGJ_000878"
     CDS             complement(24052..24498)
                     /locus_tag="ACSIGJ_000878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329785.1"
                     /translation="MQSELDLQRTVSQDFSKNVQQTNTEINQHLDKLKADKEAAETAA
                     AEALANGDMETAKRKAHEAQDAAAKADNWQQGKVILNMLASGLAEPTQSGAGIAAATA
                     SPDVSYAIGQHFKDLAGQNANGKLTASQETAHVLAHAVLGAAVAAA"
     gene            complement(24794..29934)
                     /locus_tag="ACSIGJ_000879"
                     /pseudo
     CDS             complement(24794..29934)
                     /locus_tag="ACSIGJ_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238275.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
     gene            30135..30386
                     /locus_tag="ACSIGJ_000880"
                     /pseudo
     CDS             30135..30386
                     /locus_tag="ACSIGJ_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(30381..30503)
                     /locus_tag="ACSIGJ_000881"
                     /pseudo
     CDS             complement(30381..30503)
                     /locus_tag="ACSIGJ_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation factor domain protein"
BASE COUNT         7235 a         8442 c         7512 g         7314 t
ORIGIN      
        1 caaaaaatat gcgaaacaaa ataaaccgca acgacatgga actcggctac acgccttaca
       61 accttcgcac cctgcgcaac cgctgcaaac tgacacaggc cgagttggcg caaatcgtcg
      121 gggtgaagca ctacatacag gtaggccgct gggaagccga acccgatacc gaaacgcggc
      181 gggccgatat gccgctggaa aaatggcggc agttcctcga ttggatagaa aaaacaaacg
      241 ccgtctgaaa ccttcagacg gcatttttta cccaccccca aacctaaaaa ccacaaccaa
      301 gccctacacc gcttgccccc acctcgccgc cgccccaaaa taacctcact ctgttggaag
      361 cctgccggaa acggcaaaag ccgccccgtt ccgccattac ggggcggttt cccacatcaa
      421 gacgagacag aaatgaccga ccgccaaata gacaacctga tcaaacccgc caccatcgcc
      481 gacaccgacc ccgcgtccgc gcacaacacg gcggcattac caccgactgg ctgccctata
      541 tcgtcccctt tgcaggcggc gtgtcagtat ggcgcatccc aagtgtcggc gaagcctgca
      601 ccatcttatc ccctgccggc gaacccgaga acggcgtagt tttatgctgc caagcctccg
      661 accgataccc cgccccgtcc gccgaccccg ccgaaaccgt cgtccgattt cccgacggca
      721 cgcacatccg ctacaaccac aacagcggcg cgatggaatt aaaagccgtt acaagcctga
      781 ccatcgacac cccccaaacc accataaccg gacacctgac cgtcaaccaa accaccaccg
      841 cccaagggct gctgacctac caaaatggca tgaacggcca aggcggcagc ctgtccgagc
      901 acacccaccc cgacgactcc ggcggtacaa cggaaaaacc ccaatgaccg acgcagaaaa
      961 cggacgcggg caagacaccc tcgcccacat cgcccaatcc atccgcaaca tcctgttcac
     1021 aagaatagga acgcggctga tgcgcgaaga atacggcagt tttattcccg acttgatcga
     1081 tatgcccgcc ggtcacgccg ccatcgccct catccaccaa gccgccgtta ccgccctcgc
     1141 aagatggaaa ccccgcataa ccgtccgccg catacaggca gacaccgccg acctcgccgc
     1201 cggcaaaatc aaattaaccc tagacgtaac ccttgccgac ggcggcgaac gaacctaccg
     1261 catcaaataa taaaaacaac aggataaaca atgggaaaca gccgattaag ccaactcccc
     1321 gcgcccgccg ccatcgaaga aaccgacttt gagggcatct tcgcgcgcaa aaaagccgcc
     1381 ctcaccgccc tatgtcccga aagcatccgc gaaaccgtcg cccaaaccct cgaattagaa
     1441 tcagaacccc taaccatcga cctgcaacag caagcctatc aagagctgct cgtccgcaac
     1501 cgcatcaacg aagccgtcaa agccaacctc ttggcatacg cacaaggcag cgacctcgac
     1561 cacatcgccg cccaatacgg actttcacgc aaaaccatcc gcgccgccga ccccgacgcg
     1621 aacccgccgg ttgccgccga atacgaaacc gacgacgcat tccgcgcccg cgtccaagcc
     1681 catcccgaaa aatacgccgc cgggccgcgc accgcatacg aagcccacgc catcgacgca
     1741 cacccccaaa tcacacacgc ccgcgccgtg cgccgcgccg ccggcacggt ggaggtttac
     1801 atcaaaaccc aaagcggcac gcccgacgaa accattttga ccgccgcgcg cgaatacctg
     1861 tccgccgaaa cacgccgccc cctttgcgac aacgtgcaag taaccgccgc ccaacccaaa
     1921 gacgcggcag tagagtattc cgccgaatac caccccgccg ccaatattca agccgaacgg
     1981 cagaccgcct gcgaagcctt ggataaccta tggcggcaaa acgcccacat cggcgcatcc
     2041 gtcgccctgt ctaaaatcat cggcgcgtta gacacccccg gcgtgaaaaa aaatcacact
     2101 gcacagcccc gccgccgaca tcgaatgcgg caacggcgaa tacatccgaa tcacgtccac
     2161 gctcgagcgc gaataaatga acagcaccgt ccccgcgaac aacagccccc tgcaacacgc
     2221 actggcaaag ctgacagaac gcgaaactgc cgccgtctcc cgccaactcg accccggatt
     2281 tttacccttt cacgccttcg caagaagcat cggcacggaa gagggctggg actttgccga
     2341 aactgacgaa gcccgccgca acctcatcgc aggctttgcc gaaatccacg cccgaaaagg
     2401 cacgccgtac gccatccgcg ccctcttccc catcttgcgg ctgggcgaaa tccaaattat
     2461 cgaacgcgac ggcgagttca agtgggacgg ctcggtcttg ttcgacggca gccgcacatt
     2521 cggcaggcgc aagggtgact gggcggaata ccgcattgtc ttaacgcgcc ccgtcagcat
     2581 ccgccaaacc gcccgcatcc gcgccatgtt ggcggaaatc gcccccttgc ggtgcgaact
     2641 taccgcgctc gactaccgca accatcccca ccgctggaac ggcaaaatcc gctttaacgg
     2701 cgaatacggt ttcggcacga cataacgcgc ccccgaaaat caacaaacaa aaggaagccc
     2761 caaaatggca aacgcaaccg aacaaaacca attcgaccaa gccgtccgcc tcatcgaacc
     2821 cggcgacagc gtcgtcgtcg gcccaggcgc gcccgtcaac caaccactgc aagccctcgc
     2881 caaccgcacc cttttgctca aaaaccaaac cgaagccctt cagacggcat ccgacaccaa
     2941 agccgcacca tcgcactggg cgcgcccggg caaatcaccg caacaagcca aaacaccgtc
     3001 ccaaaaaacg gccacaccca cgccatcgac accgcacgca ccgaccgcgc cggcatcgtc
     3061 cgattagaca atgccattag cgaagccgaa gacaccgccg ccacccccaa agccgtcaaa
     3121 accgcgatcg accaagcccg cgccgccgcc gcgaccgccg acctcaaagt ctccctctcc
     3181 gacaaccaaa ccgttaccgg ccaaaaaacc tttaccgccg aaacacaatt ccaaagcggc
     3241 atccggctgt ccgccaaccc gacgcactga aacggcggct acaaagccta tatcggcgcg
     3301 gataacgaaa acgcccacat cgtcttcggc gacgacaccc tgcgcctgca cggcgcaaat
     3361 aaccgcattt cctacaacaa ccacgacatc ttccacaaag ccaacaaacc gcgtttcaac
     3421 gaagacatcg aaggcaaacc gaacacgctg tccggctacg gcatcggcaa cttcaaagtc
     3481 gaaacattcc ggggcgattt gaacagcctc aaaaccgacg gcgtctattc cctgccgacg
     3541 gcggtcggca gctcgaacct gcccgttgaa aacaccgcct gccatatcca agtcatcgcc
     3601 ggcacgcaac ccggctggtg caggcagttg ggctatcccg cctacacgtc cgacgtgtac
     3661 gaacgctacc agaccagcag cgcaaacgac gactggagcg cgtggaaaaa actcaattcg
     3721 gaaggcatcc ccgccggcgc gatcgtgtcc tttcccaaag ccgtccgaaa ccccgcaggc
     3781 tatctcaaag ccgacggcac gatctttgca caaaacacct tctcgacctt taccgcgccc
     3841 tgggcaacac aaacaaactg cccgacctga cccgtaccga catcggcatc accgcgtggt
     3901 ttccgtccga ccaaatccag acgggctggc tggcgtttga cgacatccga gagcgcgtaa
     3961 ccgaatcggc ttatcccgaa ctttaccgcc tgctgaccga aaaatacgga cgcatccaaa
     4021 acgtcccgca ggcggaagac cgctttatcc tcaacgcggg caacggcttg gcggtcggaa
     4081 cgaagcagga agacgaaatc aaacggcacg tccacaaagt attttcacac tgggcaaacc
     4141 acccagacgc cgccgccgtc ggttacgaag accgcaatga aaggcaaaga agcgcgctcg
     4201 tatcgacttg gacggacggc gacttaaacg acaacggctt tttaaccccg cgattggaca
     4261 gcaaaatggc aacgggcggc gcagaaaacc gccccaaagc cctggtttta aaactgtgca
     4321 tcaaagccgc cgacaccttg ggcgaagccg tgttccggat aaagtcccac ggcgaaaccg
     4381 ccaacgccgg cgcactggac gcgtcaaggc tggcgcaagg tttgcaggaa aaagccgacc
     4441 gcgaccacac ccacaccacc gcccaaatcc aagggctgga cgaaaaaatc agcgccgccg
     4501 ttgccgcgca attcacacgc caaaccatcg gcggcgtgga tattgtcaga ttccccgacg
     4561 gcacaatgat acagaccggc agttacaggt ttgcaagagg cggcagcccc ataggaaacg
     4621 aagttgtctt ccccatcgcc tttgccgacg gcaacgtcaa atgcttcgta tccgaacgcc
     4681 attcgggacg cgtcaacggc gaaaggcaac acaactggct gtttatccgc gcaaaaaacc
     4741 acgccgccgc cattattacc aactggtacg aaagcagttg cgactggatg gccatcggca
     4801 aatccgcctc gggaaacgcc gccagcccca ccccgatagt ccccgaaata cctgaaatcg
     4861 atgaagaacc gcaaagagag agtggaagat catcaaccgg actccgaaac ccccgccgcc
     4921 accgaggcct cgacttcccc gtggggtcgt aagactagcg ggcggcaggg caggctgtag
     4981 agacgggctg tagagacggg cttcagccca ccgttccaag caatccgacc gaaaccggcc
     5041 gcgccgccaa tcctgcgaaa cctatgcccc gccaatcctg ccactcttcg ccattcccgc
     5101 gaaagcggga atccggaacc caaacgcggc aggaatctat cggaaagaat aacccgaccg
     5161 ccgtcattcc cgcgcaggct ggaatccaga cccacaacgt taaggcggtt tatcagaaaa
     5221 aaccgaaacc gaacgcccta gatttccgcc tgcgcgggaa tgacgaagag ttggggagtg
     5281 acgaaaggcg ggaacaaccg cgcaaaaagc cgccgactcc ttcagacggc atcggcaata
     5341 aaaaccgcac ggccgaaacc gcgcgggaaa ggatagccgg gcgcgcccga taagcagcgg
     5401 ccgcagggtt aagtccttgc acctgttttc atcgaagaca aaggcggttc gggaaataag
     5461 ggcgcaatat caaaagtttt gcgttgcctg atttgccaca aatcataacc ggcctgcata
     5521 ttcagccaaa aatcaggcga agtgttgagc agttggctta aacgaaccgc catctcgggc
     5581 gtaatcgaag attgggcgtt gacaatgcgc gataaggcaa cccggctgac acccaggcgt
     5641 ttcgctatat cggtaatgct tgcgccgtta atatactctt ttaaaacaag gcccgggtgg
     5701 gcaggattgt gcattttcat attattccta gtgataatcc tgataatcga caacttcgac
     5761 attgccgtcg ttgaaacgaa aagtcaaacg ccaatttccg ttgactttga accgcccaat
     5821 gtcccgacaa actgcccttg aggggatgaa gattccaact cggcacagcc atatccgaag
     5881 gcgtttgcgc ggcattcagc gcggttagaa gcagattcag tttgacggaa tgcccggctt
     5941 ggatgcccga tagcgagcct gttttaaaaa agcgttcaag ccctttgtgt ttgaaagaaa
     6001 caatcatcat cccaccccgt ctgtatcgcc aaaggataca aaacttttat ttgattgcaa
     6061 agcgacgata tacaaaaaag gaaaccccga aatgaccatc tatttcaaaa acggctttta
     6121 cgacgacaca ttgggcagcg tccccgaagg cgcggttgcc gtcggcggcg aagagtacgc
     6181 cgccctcttc gcaggacagg cgcagggcgg gcaaattgcc gccgattccg acggccgccc
     6241 cgttttaacc ccgccgcgcc cgtccgagta ccacgaatgg gacggcaaaa aatggaaaat
     6301 cagcaaagcc gccgcccgtt tcgccaactc ccgtttcgcc aaacaaaaaa ccgccttggc
     6361 attcagcctc gcggaaaagg cggacgaact caaaacagcc tcttggcggg ctatccccaa
     6421 gtggaaatcg acagctttta caggcaggaa aaagaaaccc tcgcgtggca ggcggacaac
     6481 gacgccccga ccccgatgct ggcgcaaatc gccgccgcaa ggggcataga attggacgtt
     6541 ttgattggaa aggttgtcga aaaacccgcc cgcctggccg ttgccgtcga cgcgattatc
     6601 ggaaagcggc agcaacttga agatagatta aataccatcg aaaccgcgcc agcattagat
     6661 gcgttagaaa gggaaatcaa agaatgggcg ctaaacgtcg gctgagagaa tatatttatc
     6721 atctactagt ggccatagac caactgttca acgctataac cggcggcgcg gcagacgaaa
     6781 ccctctcaag tcgcacctat cgcggcgcgc ggctcgccca acaacccaaa acccgctgga
     6841 agattttata gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaaa
     6901 tagtacggca aggcgaggta acgccgtacc ggtttttgtt aatccactat atataccctg
     6961 atcaacggcg tatttttcga ccgccaccac tgccggcagg cgtatatcag ctaactgaaa
     7021 ggcaggcagc acgacgcgcg gttcaaccaa agccgcgccg ccaatgaaag ggatacgcgg
     7081 taaaattgcc gccgcgccgc attcagacgg catcggcaat aaaaaagccc gaaaaaatcg
     7141 ggctgaaagt taaggctgca tcgcctttaa ggcggctttc gccaaaaaac cgctcgggtt
     7201 tcatgacggg cggcagtgca ggcatcgaca cggttgagga tatgttgcgg ccaactgacg
     7261 ttaaaccgca cctgtccggt taatgcggca tcgtccgctt caaccatcgt ccacaacacc
     7321 gcatcggcat aatcgggatt gtcccgatta acctctatgc tttgcgcctc gggaatcggc
     7381 aggttttcct gcaaaagccc ttcgagatgg aacattgcgg cggctttggc atcggcaagc
     7441 gcgtcggcaa ccgtatcgcc gacaggatag cagcccggca gatcgggaat aatcacgccc
     7501 cagttgcttt gatcggggtc ttggaacagc acggcgggat agtaaatcat tacttccttt
     7561 ctcggatatg cggtatcggc aaagtccaaa cgcaacgaag gggatttaaa aacgacaaaa
     7621 acgcaaaagc cgccgacatt cccgcatcca agtttcagtc aatcagataa ccttggattt
     7681 ctttggtttt cgcattgatt tctctggtac ggcagtcaga ttgtgccacc ccgtattcgt
     7741 cgccgtcggc gcatttggca tccaaaccgc tttgccagtt gcggtactgg ggcataacgg
     7801 tttcggcaat atcggcgggc agtgcctgaa ccgcgctgtt cagagccgct ttggcagttc
     7861 tttcttgtcc tcttccctga ccatttcgtc gataagggct tttttacggt tgtgcagctc
     7921 ttccaagcgt gcttcggttt ctgccgtttt acacgccaac tcactgactt ttatgccgtt
     7981 tggcgtttca ccttcggctt tcgcattgtt ggcacagatc ttgtccatac cgcttttcca
     8041 tgttttctgc gaggcttgca gcttattctg tacagtttga ggcaatcctt tccaaaactg
     8101 ctcgaactcg ctgcgcgaca gcttgtagcg ttcttcccat tcggatacgc gggcggcttc
     8161 ctgctcccga cccaatgcct cctgcgccgc cgcttcttct gcctccctcg cttcctgcgc
     8221 ggtggcttct gccgcttcaa gtttttcgtt cctttgtttc gctttgtcca acggctcttt
     8281 aatcagtgcc atagacacca gctcgctgag cggatggatg atatcgtgtg cctgactgag
     8341 ttcggcaaag attttgcttt tgtcgtctgt cggctggacg ctgtaagaaa tagttttgac
     8401 ataatacgcg ccttctttgc ggtagtaggg ttcaaaaaag tcgggcgttt tcttatagct
     8461 gttgcctata gattggttgg cggcgacggc ataatcgaca acatcgtcgg gcacgtccag
     8521 ttttaacgcg gcttcacatt tgagtttgct ggacgtgtcg gttttctcgg tagttcggac
     8581 ttcatcgacg gttatgccca aacgttcgac cattctgcgg atatgcgata tgccgatatc
     8641 ggcaaatgct tggtctttga atgcgtcgtc gtcaaaagtt ttgacggttt caccttcgac
     8701 attgtcgcgg accagtttga ccaaaatctc tttagtctct tcgcttccgc aggaaatttc
     8761 ctttccggaa aacgggttgc ctattcccaa acggtcgcag cccgacaaaa tcagcatact
     8821 tgccaatgcc agtgcgggta aatttttttt cattagattt cctttgccgt ccaaacacgg
     8881 actgtctgtt gcgtatttcc acacacgcta cggcacttga agtacatggc tgcggaacat
     8941 atatgtttaa atttatgcaa accgccatat cgcgattgca cacgccctgc aaatttaccg
     9001 acggttttcc tgttcgataa aaatgccgtc tgaaacggtc ggcgttcaga cggcattttt
     9061 ccgcaggttt tatttgcggt tggtctgcag gtagaggtta atcaggcgtt cggtcgagct
     9121 gtcgtgtttt tgaggttcgg tttcgcctgt cagttcgccc aaaatggttt tagccagttg
     9181 tttgccgagt tccacgcccc actggtcgaa gctgttgatg ccccaaatga tgccttggac
     9241 gaaggttttg tgttcgtaca tggcaatcag gctgcccata ttgcgggggt tgaccttatc
     9301 catcaggatg aggttggtcg ggcggttgcc ggagaagatt ttgtgcggaa ccaactcttc
     9361 gatgcgcgcc tcatccatac cctgcgcttt gagttcggcg cggacttcgt cgggggtttt
     9421 gccgcacata aaggcttctg cttgtgcaaa gacgttggca agcaggattt cgtggtgtcc
     9481 gggcaggttg ctgcgttttt caagcgaggc aatcaggtcg atgggggtga tgtgcgtgcc
     9541 ttgatgcagc agttggaaga aggcgtgctg accgttaatg cccgtttctc cccagataat
     9601 cggcgaggtt tcgtgtccga ctgctttgcc gtccaacgta acctgtttgc cgttgctttc
     9661 catatcgagc tgctggatga atttgggcag gcggtgcaaa tgttggtcgt aaggcgcgat
     9721 gacgtggctg ccgccaccgt agtagttgat ataccagatg ccgatgaggg cgaggatgac
     9781 gggcaggttg cgctcgagcg gggtgttgat gaagtgtcgg tccatcaggt gcgcgccgtt
     9841 gagcatttcg atgaagtttt cttcgccgag atacagcata atcggcaatc cgatggcgga
     9901 ccacaggctg taccgaccgc cgacccaatc ccaaaattca aacatattgg cgatgtcgat
     9961 gccgaattcg gcgacggctt ttcgattggt ggaaacggcg gcgaagtgtt tggcaacggc
    10021 ttcttcgtca cccgcatgat tcaaaaacca ttcgcgcgcg gttagcgcgt tggtcagcgt
    10081 ttcctgcgtg gtaaatgttt tggaggcgat gatgaacagc gtggtttccg ggtggacttt
    10141 ggacaatacg tcgcgcagtt gcgagccgtc cacgttggag acgaagtgca tattgaggcg
    10201 cggatgaccg aaaggtttga gcgcggtaca catcgtcagc gggcccaaat ccgatccgcc
    10261 gatgccgatg ttgacaacgt cggtaatgac ttggttggta tagcccagcc agcttccgct
    10321 gcggacttcg tgtgcaaatt cgcccatacg ttgcaaaacg cggttgactt tgggcatcac
    10381 atcttcaccg tcaaccataa tcggcgaatt ggtgcggttg cgaagggcga catgcaggac
    10441 ggcgcggttt tcggtggtat tgattttttc gccgtggaac atctgccgca tccgctccgg
    10501 cacgcctgct tcacgcgcca actcgaacag tagggacatg gtttcgtcgt tgatgcggtt
    10561 tttggagtag tccagcgtca gtccgccgac ttgcagccag tagcgttccg cccgctgcgg
    10621 gtcttgctcg aacatttcgc gcatatgcaa tgttttgctg tcgtcaaagt ggttccacaa
    10681 ttttgaccag gccggtaagt cgtgaaggtg tttcatctgt atgctcccga ttgaggtttt
    10741 ccgtgacggg gggcgtttcc gcacatcgat gcgcacataa ggcgcaaccc tgcgtaccgc
    10801 cccgatttgg aaggggttgc ggcaaacccg aaccctgccg ccttaaaggg cggggtttcc
    10861 gccattaagg aaattctgat aaaaggcttg ccggagcctg tccggccgtt cagacggcat
    10921 tggcgggatg ccgtctgaac atcagtcttt gtcgtaatcg atgtgcttgt tgtgtatgct
    10981 ttttttgctt ttctgcaatt gcaggctggc agcatcgccc aagcgcaggg caagtccgat
    11041 ggcgagaata tcgatgacgg caagctgcaa gaggcgggaa accatgggcg tgtagagttc
    11101 ggcgttttcc tgtgtggcaa cgctcaacac gcagtcggca agttgcgcca gaggcgaatc
    11161 gttgcgggtc agtgcgatga cggacgcgcc gttttctttg gcgatgctga ccgcatccaa
    11221 aagttcgata gacgaacccg tgttggaaat ggcaaccaaa acatcctgat cgctcaaaac
    11281 ggatgccgcc atcagttgcg tgtgcgtatc gacataggcg acggtggaca tgccgaaacg
    11341 gaaaaatttg tgctgcgcgt cctgtgccac aatgccggaa ttgcccacgc cgtaaaattc
    11401 gacgcggcgg gcgtgcatca gggtggcgat gccgttttcg agttcggatt cttttaagaa
    11461 cctcctctcc cccaacaagg ctgcggcggc gttgcccaac actttttcaa ccacattcgc
    11521 catatcgtcg tcggcattga gttcttcgtg gacataaggc ataccttcgt gcccgatgct
    11581 ggcggacagg gagagcttga attccggcag ccccttataa ccgaggctgc ggcagaaccg
    11641 gatgacggtc ggctggctga ccgaggcgcg gtcggcgatt tcggcgacgg cggcgtggac
    11701 aaaccacttg ggttctgcca aggcgcattc ggcaactttg cgttccgcac cggacaggtc
    11761 ggccagtgat tcgctgattt tgcttaacat aatgatatgc ccttcgataa tgcggccctg
    11821 ctgcaagggc tgccgtggtt agacgttttt caggtggttg ccgagcgccg ccgccgcgcc
    11881 ggcaataccc ggaaattcgc tcaagacgac ataaacggga atcgcggcaa ggtaggcttc
    11941 aaaacgccct ttgttttcaa agcggctgcg gaagggggag gttttgaagt aatccaacat
    12001 tcgcgggata atgccgccgc acaaatacac gcccccgcgc gcgcccaaag tcagggcgag
    12061 gttggaagcg accgtgccca gcatcgcgca gaaaatgtcc aatgcctgcc ggcacagggg
    12121 cgattcgcaa tttaatgcct tttcggtgat ttcagacggc atcagttttg ccggttcggc
    12181 tttctgcttg gccgccagcg tttcataaat caggctcaaa cctgcgccgc tgaggaaacg
    12241 ttcggcggaa acgtggcggt atttgttttt ggcgtactgc caaatcaaca cttccatatc
    12301 gtcaaacggc gggaaactgg tatgcccgcc ctcgcccgcc aacgccaccc agcctgcggg
    12361 gctgtgcacc aatccgctca cgcccaagcc ggtaccgggg ccgataacgg ccttgggggc
    12421 gaattcgaca ggcttttgcc cgcctacctg catcaggtct ttgcttgaag tctgcgttac
    12481 cgccaatgcc tgcgcggtaa agtcgttcaa aaggatgagg gtgtccagcc ccaaagcctg
    12541 acgggtggtt tcgatggaaa acgcccaatg gtggttggtc atctgcaccc agtcgcccaa
    12601 aatcgggttg gcgatggcaa atgccgcgtg ccgtacgccc gttgcaccac tttggttcag
    12661 ataggcacgc accgcgtcgg taaccgtgtc gtattcctta cacggcagga cggcgacttt
    12721 ctcaatcacg cacggcgcgg tttccaatgc gaaacgggcg tttgtgccgc cgatgtcggc
    12781 aaccagcctc gggtaatcgg cgtgtttatt cggcgtagaa gacatggcag ttcactcctt
    12841 gatggttcaa aacgaggctg atcggatatt cgcggttttc gccttgtgcg gcttggtcga
    12901 acacggcttt tttctcttcg ccctgtatcg ccaaaaatac atgcccagta tgggcaatcg
    12961 catccaaagt catgctgatg cgctcgtgcg gcgcggtaac gggcgtggta tgcaccagcg
    13021 cgacacctgc cgaaccgtcg attgccgtct gaaactgcgg agcttttggg aaaatcgaag
    13081 ccgtatgccc gtcgtttccc atacccaaaa tcaaaacatc gggctgcttg taatgtttca
    13141 gcgcataatc gacaacagca tcgggatgta attcggtttc agtttttccg tcttccacca
    13201 taggaatcca cactgccgca gcggctttgt tctttaacag gtattcgcgc accagaccgg
    13261 tattgctgtc ggcgtggttg gtcggcacga tgcgttcatc tgccaaggta attccgacgt
    13321 ttttccaatc caaatctttt tgcgacaggg cgttgaaaaa tgcaatcggc gaacgtccgc
    13381 cggaaactgc caacaccgca ccgcccttct cgtccagtgc gccctgcaaa gcatccgcca
    13441 ctgcgtcagc caaagactgc gccgcttctg ccgcattttc gtattcgtgc caaacaaaca
    13501 tatttgtgtc cttttgtgat ttcagacggc atatcccgtt acagggcggc tgccgggcaa
    13561 tggcttagcc gcttccgcct cgaagcggga ctgctttttc tgccccgccc tccttcagac
    13621 ggcattgcga acccgatgcc gtctgaagcg cattactgtt cttcgtgcca cttgttgccg
    13681 tcgcgcgcca acagttcgcg ggcggcttca ggaccccaag agtgtgcgcc gtaaccgtgc
    13741 ggcggcgtgg tgttatttgc ccagttttcc aaaatcggca tcacatattc ccacgcggct
    13801 tcgagttcgt cgcggcggtt gaacaaagcg agtttgccgt taatcacatc caacagcagg
    13861 cgttcgtaag cctcggcgcg gcggccttca acggccttgc ccaaatccac gcccaaaggc
    13921 gttacttcca ctttgttgcc cgcacccggg gttttcacct gcgtgtaaag gcggacggat
    13981 tcattcggtt gaagctcgat aaccaaacgg ttcggcgcgg tttggctgtt gtcgaaaata
    14041 tggttttgca gcgggcggaa gttcaaaacg atttccgcca ctttgcccgc catgcgtttg
    14101 ccggtacgca ggtagaaggg aacgcccttc cagcgttcgt tttcgatttc ggctttaatg
    14161 gcgacgtagg tttcggtaaa gctgtcttgc ggaacgttga tttcttcaag atagccgttc
    14221 atgcctttgg cagcggtata ttgtccgcgc acgacatttt cattgacaga ttcgatggtc
    14281 agcggcttca atgacttgat gactttgact ttttcatcgc gcaccgcgtc ggcatccaag
    14341 ctggcggggg cttccatcgc agtcatgcac agcatctgca tcaaatggtt ttgcaccata
    14401 tcgcgcaacg cgccggtaat gtcgtaaaac tcgccgcgct cttccacacc gagctgttcg
    14461 gcgatggtca gctgcacgct ttcgatgtat ttattgttcc acagcggctc gaacattaca
    14521 ttggcaaaac gcagcgcaag caggttttgc aggctttctt tgccaaggta gtggtcgatg
    14581 cggtaaattt gcccttcttt gaaataacgc gccacatcgg tattgatttg ttgggaagaa
    14641 gccaaatccg tacccagcgg tttttccaaa accacgcgca cattgtcggc attcaaaccg
    14701 atcgcagcaa ggttttcgca ggcttgcgcg aagaatttcg gcgcggtgga cagatagatg
    14761 acgacgttgt cggtttcttt gcgtgctttg accaaatcgc ccaaagcggc aaaatcgtcc
    14821 ggctgcgtaa catcgacttt gagatatgcg agacgttcaa caaacgaagc ccacgcctca
    14881 ccggaaaaat tttctttcac atggattttg gaactggttt ccaccttcgc cagaaaacct
    14941 tcggtatcca actcgctgcg gctgaccccc aaaatacgcc cttcgggatg aagcagaccg
    15001 gcaacatgcg cctgatacag gcagggcaaa agtttgcgca ttgccaaatc gccggtcgca
    15061 ccgaacaaca ccaaatcaaa atttgtctgt gtactcatcg cattatctcg tcaggaaagg
    15121 attttttcga tgccgtctga aacctgtttt ttccatcacg cagcatcgca atatcggaaa
    15181 caaaggcagg cggcataatg agtagtaata ctacacaccg ctacactttt tgtctattcc
    15241 catttttaca atttatttga cctagtccaa aaatcaggca ggtttcccct attccgttac
    15301 aacaatcaga agattctgcg atttaaatca aatttctttt caatgtctga tttttttgta
    15361 acaaaattac aaattttgta ctataatagc acccgcttcc cactttcaga cggcgtacct
    15421 tttaaaatat agtgaattaa atttaaatca ggacaaggcg gcgagccgca gacagcacaa
    15481 atagtacggc aaggcgaggc aacgccggac cggtttaaat ttaatccact atactcaccg
    15541 tctaacatac ccgatacaaa aatcagaaac gcacaaacaa atccccaata ccaccccgtt
    15601 ccgccaggag accgaacatg aaccacactc ctatccaccc caaactcgcc gaaatcaccg
    15661 ggcgcattat cgaacgcagc cgtccgacgc gtgaaaaata tctggcgaaa atccgcagtg
    15721 ccaaacaaat gggacgctta gagcgcaacc agctcggctg cagcaatttg gcgcacggtt
    15781 atgccgccat gcccaaaagc atcaaaatcg aaatgcttca ggaaaccgtc cccaacttag
    15841 gcatcatcac tgcctacaac gacatggttt ccgcacacca gccgtttaaa gacttccctg
    15901 accaaatcaa agacgaagca caaaaaaacg gcgcgaccgc acaagtcgcc ggcggcacgc
    15961 ctgccatgtg cgacggcatc acgcaaggct acgccggcat ggaattgtcg ctgttttccc
    16021 gcgacgtgat tgcgatgagt accgccgtcg ggctgtcgca ccaaatgttt gacggcagcc
    16081 tgtttatggg cgtgtgcgac aaaatcgttc cgggtctgat gataggtgcg ctttcgttcg
    16141 gtcatattcc cggtatcttc gttccggcag gcccgatgtc cagcggcatc ggcaataaag
    16201 aaaaagcccg tacccgccaa cttttcgccg aaggcaaagt cggacgcgac gaacttttga
    16261 aaagcgaaat gggttcttac cacagcccgg gcacctgcac tttctacggc acggcaaact
    16321 ccaaccaaat gatgatggaa atgatgggcg tacacctgcc tgccgccgcc tttgtccacc
    16381 cctataccga cctgcgcgaa gccctgaccc gctacgccgc cggacacctc gcgcgcggca
    16441 tcaaaaacgg cacgattaaa cctttgggcg aaatgttgac cgaaaaatcc ttcatcaatg
    16501 ccctgatcgg tttgatggcg accggcggct cgaccaacca caccatgcac ctcgtcgcta
    16561 tggcgcgcgc cgcaggcgtg attttgaact gggacgactt cgacgaaatt tcttccatca
    16621 tcccgctgct catccgcgtg tatcccaacg gcaaagccga cgtgaaccac tttaccgcag
    16681 caggcggcct gcctttcgtt atccgcgaat tgctggacgc aggcttattg cacgacgatg
    16741 tcgataccgt cgtcggacac ggtatgcgcc actacactaa agagcctttc ctcatcgacg
    16801 gcaaactcga atggcgcgaa gcccccgaga ccagcggcaa cgacgacatc ctgcgtaaag
    16861 ccgacaatcc gttctccccg gacggcggtc tgcgcctgat gaaaggcaac atcggacgcg
    16921 gcgtggttaa agtgtcagcc gtacgcgaag gctgccgcat catcgaagca cccgccatcg
    16981 tgttcaacga ccagcgcgaa gtgttggcgg catttgaacg cggcgagttg gaacgcgatt
    17041 ttatctgcgt cgtccgctac caaggcccgc gtgccaacgg tatgcccgaa ttgcacaaac
    17101 tgaccccgcc tttgggcatc ctgcaagacc gcggcttcaa agtggcgctg ctgaccgacg
    17161 gccgtatgtc cggcgcgtca ggcaaagtgc ccgcgtccat ccacatgacg cccgaagccc
    17221 tgatgggcgg caacatcgcc aaaatccgta ccggcgacct gattcgcttc gactccgtta
    17281 ccggcgaact caacgtcctg attaacgagg ccgaatggaa cgtccgagaa gtcgagcgca
    17341 tcgatttggg cgcgaaccaa caaggctgcg gccgcgaact cttcgccggc ttccgcagta
    17401 tgaccagcag tgcggaaacc ggtgcgatga gcttcggcgg agaatttgcc tgatgcgcgt
    17461 ttcagacggc cttttaaaac agaaggccgt ctgaaaaatt attgaagccg tttaaaatag
    17521 atgcagacgt agcgtgagct ttgcccgcga acaaaaccca agcaacatca gaatgtttta
    17581 ccccaaaggt tgtctgaaaa cttaaccccc ccatcccttt tgacctcaac aaaaacgaca
    17641 ccccccactt ggagaaccga aatgtccaaa ctgacccccc gccaaatcct gaccgccggc
    17701 gcagttgtgc ctgtgatggc gattgacgac ttaagcaccg ccgtcgattt gtcccgcgcc
    17761 cttgtcgaag gcggcatccc caccctcgaa attaccttgc gcacccccgt cggactcgag
    17821 gccatccgcc tgattgccaa agaaatgccc aacgccatca tcggcgcagg tacggtaacc
    17881 aatcccgaac agctcaaagc cgtcgaagac gcaggcgcgg ttttcgccat cagcccgggt
    17941 ttgcacgaat ccctcgccag agccggccgc aacagcggca ttcccctgat tcccggcgtt
    18001 gccactccgg gcgaagtcca actggctttg gaacacggca tcgacacact caaactcttc
    18061 cctgccgaag tcgttggcgg caaagcgatg ctcaaagccc tctacggacc ttacgccgac
    18121 gtacgcttct gcccgaccgg cggcatcagc ctcgccactg cgcccgatta cttggcattg
    18181 cccaacgtct tatgcgtcgg cggctcttgg ctgacaccga aagaagctgt aaaaaacaaa
    18241 gactgggaca ccattacccg cctcgccaaa gaagcggcgg cattgaaacc caaagcctga
    18301 accgcatcgt aaaaatgccg tctgaagccc tttccagtct cagacggcat tttgccgctt
    18361 ggcgactcag tcggcataca cagcagcacc gcccctaaaa tgcccatcca tctccagaac
    18421 aatgcgaatg acgcgcgata tgcggagacc tatgacgaaa aaataattat aatatattta
    18481 tcaattagtc ataaaaatcg ccctatcggt aaaatgccgt gatgagcggc tgaaactaac
    18541 aagttaaccg tcgttaaaaa ataacgctca gagacctttg caaaattcct tttcttcaaa
    18601 cagccgacat ccaaccacag gtttttcggc tgttttcgtt tcaaataacc gccgttccca
    18661 ccaaaatatc ccattaatcc cccttaaata tagtggatta aatttaaacc agtacggcgt
    18721 tacctcgcct tgccgtacta ttttactgtc tgcggcttcg ttgccttgtc ctgatttaaa
    18781 tttaatccac tatacctgat tgatcaggaa cagcccgttt tacacggctg aaaaaatgcc
    18841 gtctgaaaca cgttcagacg gcatccgctt aaaaaacaac tgattcaacg gcgattaatc
    18901 cgcttccaaa accactttca tcgcttggtt ttcggcggcg tgtttgaaca cgtcgtaggc
    18961 tttttccaat tcgctgaatt tgaaacggtg ggtcaacatt ttggtgtaat ccactgagct
    19021 gctgaaaatt gccttcatca gtatttcggt ggtgttggca ttgaccaaac cggtagtgat
    19081 ggcaagattt ttaatccaca gtttttccag tttgaaatca acggattgac cgtgtacgcc
    19141 gacgacggcg atatgaccgc cgggtttcac gatgtcttgg cacatattcc aagttgcagg
    19201 gataccgacc gcttcgatgg cacaatctac gccgtcttcg ccgacgatgg cgaaaacttg
    19261 tttggagacg tcgccggaag cggggctgat ggtatgggtc gcgcccaact ctttcgccgg
    19321 tttcaaacgg ttttcgtcca tatcgcacac gataatggcg gcagggctat acagttgggc
    19381 ggtcaacaag gcggacatac cgacagggcc ggcacctgcg atgaatacgg tatcgccggg
    19441 tttcacatcg ccgtattgca cgccgatttc gtgggcggtc ggtaaagcgt cgctcaacag
    19501 cagggcgatt tcttcgttga cgttgtcgtg cggcggcacg aggctgttgt cggcataagg
    19561 cgtgcggacg tattcggcct gcgtgccgtc aatcatatag cccaaaatcc agccgccgtt
    19621 gcggcagtgc gaataaagtt ggattttgca gttgtcgcaa gtgcagcatt tgctgacgca
    19681 tgaaataatg actttatcgc cgactttgat gttttttaca gcctcgccga cttcttctac
    19741 aataccgatg ccttcatgac cgaggatacg accgtcggcg acttcggggt ttttgccttt
    19801 ccaaataccc aagtcggtac cgcaaatcgt ggttttgacg attttcacca ccgcatcggt
    19861 cggatcgata atctgcgggc ggggtttttc ttcaaaacgg atgtcgtttg cgccgtgata
    19921 aaccattgct ttcatgctga tactccttgc ttgttgataa ataatttcaa taccacaata
    19981 aagtttcttt atatgagtta tatgccccta caaaaaataa gtcaataaga attattttca
    20041 caattttata caataacata ctgttttaaa tataaataaa accaccgatt gatattaatg
    20101 aacacaccca tccccttctc cgaacggctc atccgctggc aaaaacaaca cggtcgccac
    20161 cacctccctt ggcaggtcaa aaacccttat tgcgtctggc tttccgaaat catgctccag
    20221 caaacgcagg tcgccgccgt gttggactac tatccgcgtt tcttggaaaa attcccgacc
    20281 gttcagacgc ttgccgccgc gccgcaagac gaagtgttgt cgttgtgggc gggcttgggc
    20341 tattacggcc gcgcgcgcaa tctgcacaaa gccgcgcaac aaatcgtcgg acaattcggc
    20401 ggtacgtttc catcggagcg caaagacttg gaaacgctct gcggcgtagg cagaagcacc
    20461 gccgccgcca tttctgcctt tgctttcaac cgacgagaaa ccatcttgga cggcaacgtc
    20521 aaacgcgttc tctgccgtgt ttttgcccaa gacggcaatc cgcaagataa aaaatttgaa
    20581 aactcgctct ggacacttgc cgaaagcctg atgccgtctg aaaacgccga tatgcctact
    20641 tacacgcaag gtttgatgga tttgggcgcg accgtgtgca aacggacgaa acccttgtgc
    20701 cgtcaatgcc ctatggcgga catctgcgaa gcaaaaaagc aaaaccgcac cgccgagctg
    20761 ccgcgtaaaa aaactgccct cgaagtgcaa accctgccgc tttactggct gattgtccgc
    20821 aaccgggacg gcgcaatctt gctggaaaaa cgcaccgcca aaggcatttg gggcggactg
    20881 tattgcgtgc cgtgttttga aagtttgaac gggctttccg actttgccgc caaactctcc
    20941 ctgacgatgg cggatatgga cgaacaaacc gccctgaccc accgcctgac gcaccggctg
    21001 ctgatgatta cgccgtttga agggcaaatg ccgtccgaac accattcaga cggcatttgg
    21061 ataaagccgg gccatttgaa agattacggc ctgcccaagc ctttggaaat ttatttaaac
    21121 ggtaataggt tagaataaac aaaataaacc cgttgaactg ttgttagcag gtatcgcagc
    21181 aagaacaacc gatgaatttg ggtcgtattt taggcagcgg gataatgttc aaatgggaca
    21241 tttggaacgg aaaaagtcgg caatttaaaa aggattttag aaacaaagag ggtcaaaaac
    21301 atgaacacaa acttaaatga caaagacaaa gccatggata ccgcaatcag gtttcagaaa
    21361 aggatgagga ttccgaaatt tttcttttta attctcggaa tcacagtggt tttggcattt
    21421 atccaagacg tgataacggg ttctaatttt ctgcaaataa caattatgta aataatattt
    21481 gtgatatatt tcagtttcgg aggtatcata attttattct acataatcat cgcaggaatc
    21541 tgcccctatt gccacgaatt ccaaaagatg aattatggca cctccgtcgg catcgggtcg
    21601 gatagcttta ctttctttaa aggcatttcc cctgttccaa aaatcgatta ctgcagcaaa
    21661 tgcggtaagc ccctgtctcc agaagccgta aataaggtga tgaatgataa aaccgatgaa
    21721 gaaatgtgaa cagcgtttta acaacagtga aataaaatat aaaatttcaa ttttaaaata
    21781 atgagctaat ggaaatttga cccggttgtc cggtacggcc ccaaacataa aacaaaagct
    21841 gcctgcaaaa aaccggcagc ttttgtgtat cttgtcatac attattcact tcaaccgcat
    21901 caaaacgaat aacccacctg aaaccccgtt acccatttct tcgtctgaaa atattcgggc
    21961 tttttcaatg cacggccggt aaatatatcg taatgcaggt tgccgccaag ctttatctgc
    22021 ccgcgtatcc caattgctgt gccggctaga gtttggcccg ataaccattt ggcggattgt
    22081 cctgaaacat gtcctacatc agccccaaga taaagctgat ggcctggttt aaattgccag
    22141 ctcaaatcgt tgcgccaata ccatccccgc tcggcaggca aactcatttc accgtcgaag
    22201 ccacgtacgg tgtggtgtcc gccgatagcc agtttgtctt gcgatgttag cggggttttg
    22261 ttccattgtg catgaacgga tgtgtcatag gcaaatagct gtttaccgat ttgaaaagga
    22321 gtatttacat cagccgatgc cgtccaaatt ttcatacgtg acgtgccttc gccaaaggct
    22381 tcttcaggcg cgcgcagagc atctttcatg ccggtgccgt gtttatattt caacttaaaa
    22441 tctgccgtac tgcgaccgat atatcctttg tgggaaagtt ctgccaacta acctgtggtt
    22501 ttacgccgtt gtacagtcag ttcggcatca tcaatgtaac tttttgtttc cctcgtccac
    22561 agttttacac tgagataggt tttgcgtttg gcatcacgat acaacaggcg gttgaagccg
    22621 aaatcagtgt tgtaactttt tccattatag tcatagactt ccgataatcc ggaaaccgcc
    22681 tgatggtaac ggtagccatt gtgattgaat gcccatgtcc atttaccgaa aggggctgaa
    22741 taatgtacgg cgtaattgtt tgatccgcct tctttgcgat ggccgtcaaa attttcctca
    22801 tcgggcgtac cgccaattga acgtccataa tttacataga acatatcact cagtccaaaa
    22861 ggattgtcgg cagagaaagt gatatttcct tggtattttc ctgtcgcctc actacccgaa
    22921 ttatccatcc ccacactcac acagtagggc agcagacgtt accgccattg caccacgaca
    22981 tcactttggt ttggttctct ctctacggga acgatttgga gatcggcttc cgcagtcggg
    23041 agacatttga gattttccag tccttgttcc aaatcacgca gattcaacag atcgttcgag
    23101 cgggtgggaa atttgttttg gaatgctgca atacgtcctg catgggtttg atcatcgttg
    23161 gaccgatcga ttcgtatgga gcgcagatag cccggcatca gggttaattg aagcttgcca
    23221 ctattcaaat cctgtggcgc agccaagata cgggtcgtgg tatatcccct gccgatcaaa
    23281 gcattttgtg ctaaggacat gatttgatta atgtcgcccg catgcagaca cttgccggaa
    23341 acaaaatgtg tttggcacaa ggcatggttg agcgcaaacc ggaatttagc cgcttcttca
    23401 cccaccaatt ccacctcaga aataggaaag caggggcttg cctcatccga atttgcaccg
    23461 cctacctgtg ttgccatctt ctctatgccc gtatcggtac catccaaacg aacatcttgt
    23521 tccggctgca tctgttcacg caattgcttc tcacgttgac gttggagaat atcctgttgt
    23581 tgtgaaacag cttcaggtgt gggggcggct gatgttaggg tggggaggca gccatcaatg
    23641 ataggactaa cagtgataag ggatagggat gaaatacatt tttggaggaa aagaaaggtt
    23701 tcataggatt ggtctaagta atgtaagcgg gcaattatag caatgcacaa aaacaaacaa
    23761 aaagataaaa aaactccata aatgaaaatt taatcacatg tttataggat atgaaaaaga
    23821 ggaagcgaaa tctgaagccg tctgaaaaca accccacaca gcatcggcgg acgagtttcc
    23881 ctcagctgct ccggcagccg tacccagcat ccttgtaatc gccgaaacgg tctctttttc
    23941 ctccgcattc aggctgccgc cgtctccttt gccgtacagc catttgccga tgattggggc
    24001 ggccgcttcc gacccgcccg cacccaatgc tcctgccggg gcattgttgc ctcatgctgc
    24061 ggcaaccgct gctcctaata ccgcgtgggc aagaacgtga gcggtttctt gactggcggt
    24121 tagtttgcca ttcgcgtttt gaccggctaa atctttaaag tgctgtccaa tcgcatacga
    24181 tacgtctggc gatgcggtag ccgcagcgat gcccgctccg ctttgggtcg gctcagctaa
    24241 acctgaggct aacatgttga gaatgacttt gccttgttgc caattatctg cttttgctgc
    24301 cgcatcttga gcttcatggg ctttgcgttt ggcagtttcc atatcgccat tggctaatgc
    24361 ctcggctgct gctgtttcgg ctgcttcttt gtctgctttg agtttgtcta aatgttggtt
    24421 aatctcggta ttggtttgtt gaacattttt actaaaatct tggctgacgg ttctttgtaa
    24481 atccagttca ctttgcactc tatctttgtc aaatatgttt ttcaggctgc ccgaatgtcg
    24541 ttcggcggtg tctgtggtta cgtttgtatc aatatcggct ttggtttgtg ccgctatttt
    24601 gcctgtcagc ctgatttgtg cggcttcgtc tgtgatttga atgtttttgg gtattgatgc
    24661 cgctttttgt gatgctgctt tgactgtcgc tgtcgctgcc gtaccctact gatgaacttg
    24721 cgccgttttt atcggctacg cttgtcaggt gtttgtcttg aggtttattt tttgcgccct
    24781 gtcccagtgt tttgccgctt acggcaacgc tggcacccaa tccaaaactt tttccttcgt
    24841 attggctgta attttgaata tcactgccgg cgagtgtgcc tgtgctgaat cggtttttac
    24901 ccttgtcttt tgcgctttgg ctgctggtga tgatgccgcc tttgaggcct gtatggtttc
    24961 cgaccttgat ttgatagccg tcttctccgg cataaatacc gctttgctcg gttaccgaag
    25021 catggtcggc tcggattttg ctttggctgt aatcgccact ggcactgaag ccataaccta
    25081 cggtaacttg tgcaccggcg ttttgttgtt tgctttgata agtttctcta tcttgtacac
    25141 tttgaatgct taggtttttg gcattgactt gtacgccttt gccgcgtact tgcgcgcctt
    25201 tgatgatggt atcgccacca ctttggataa gggtttggct gcctttgtcg ccaatatggc
    25261 tatggcggtg ggttacgcta tcgccattgc cgtagccttt gccgacattg ccgcctgcgg
    25321 caacgcctaa tgaccagcct ccttgtccga atgatacggc agcacctgcg ttccagcctg
    25381 ctgatttgtt ttggctgcgt tcggtattgc tttgctcggc tgattgcaga tggatttgct
    25441 tatcggcaat caggccggtt cctgctctgc ctgaaacacc tgagcctgtg atgttgatat
    25501 tggattgttc gccgcacctt cggcaataag ggtagttttg ccgcctgctt gaatttgact
    25561 cgcttgggct tgattggctt gaacttgggt ggtttgtcgg ttttgctgtt cgccgtaggt
    25621 tatggagatg ctgacttgtt tggcattggt tgtaccattg gctaagtttt gtgcgccttt
    25681 gcctgtttga taggcctgcc aacctgcatt ggcagccgcc atggcattaa cgcggtcgtt
    25741 tttgctttgt ccgacttgtt tgctgctttg tgtgctacgg caatcgcttg ttgtgccaaa
    25801 tcggtaacgg gcgaactgaa tgccaccgtt aagccttttt gttcgtaggt ttgggtggtt
    25861 ttgctgttta attggttttg tgctgcgcca atatccatac tttgcgtgct gataaggttg
    25921 ttgccctcag ggctggaaac gttgctgccg gtttgttcgt agtgtttgct tgcaacaatg
    25981 gtggtatcgc ctttcaggct gcctacggta ctgcctgtat gttcgttgct ttgggattgg
    26041 ttttcttgtg tgtttgtctt gctgccaata gtgaagccga tacctgcact catcaatcct
    26101 gatttttggg tttgatgata ggtttcgctt tggctttgag tttgggttgt accaatgcga
    26161 acatgattgc ctgcttgaat ccgggtgcca ttatcggaaa taacattact gccaaggatg
    26221 ttggcatcgt ttcctgcctg caatacaact tgcttgcctt caaaggtgct gctttgagca
    26281 gtttcgtgat gactttgggc tttatcggta atgactaatt tattaccgcc gccgcttctg
    26341 cctgtatgtt tggacgcatc atcaacttgg ccggcatgga tgcctgagct gatagtaatg
    26401 tcatttttag catacacggc aagtgtgcct tttgcgctgc cgacttcggc agctttggca
    26461 ttgagattat tccctgacaa tagggtaaca tcgccttttg tttgaatgct gctgccgact
    26521 tcgttcgttg aacctcggat ggtatggtta tcggcatcaa aatggatttc ttgatatttg
    26581 ccggtttgta ccgtatccag gttaatgtcg cgtcctgcct gcagccgggt ttgcccttga
    26641 tctgattgat tgctgatttg accggcaatg atgttgatgt ctttgcctgc ctgcgctgct
    26701 aaaacacctt tttctttgcc tgtgatataa atacctgcca ttcggtctag gtaggtgctg
    26761 ctaccttgtg cattttgact gctcttggcc gtgctttggt tgttgatgtt gttacccgca
    26821 ttgagcaata atgtctgttc ggcagaaaga atgccgccaa tattattgat gtcttgtgtg
    26881 gccgtaaccg ctgatttttg cgcatgaata cgcccaccga tattgtctag cgtatcggta
    26941 ttgataataa gcgcattgcg ccctgcaatc gtgcctgagt ttttcaggct gcctgaaaca
    27001 ttgatttgtg tattgctgcc tgacaacaat gcacctttac cgtctatgcc gccattttta
    27061 acgcgtacat aaacctgtgg catcaatacg gtttgtgtgc cgccatcagg aagtttaact
    27121 tctttttgta ccaaccaaac aatatcgctg gtcagttgcg ctgcttgctc ggcacttaat
    27181 gcaatgccaa cgctgagatt catcgaacgt gccgcagtcg cgccattatc cattaaggct
    27241 ttaaattgtt cttcgtcgtt ttgataaccg tctaaacgac gatgccctgt cagctctgcg
    27301 atttgttcat tgattaaacg ttgctcgtaa taaccatcac ccaaacgttt atgtaaattg
    27361 tttgggtcta gtttgaggct gcccagcata tagtcactac ccaaccattg acggtagttg
    27421 gcaaagcgtg gatcggtttc aacaagatag cctttattgg caggattgat aatgtataag
    27481 ctgctgccgg gtaatggggt aaaagaattg ggcgtatagg gtagcgaaat accgttgctt
    27541 ttcgcagtac ggatattatc ccggttactt tgtggcaact cagtgccttg gctgggcgca
    27601 tgacggctta atgctttgct atgcgattca taggcaaatg aacccagtga aatgtcgcgt
    27661 gtgatttcct ccggcaaagt ataattttgt tcacgatgcc ctgtttcatc atgtccttta
    27721 cgacgcgcac gccagtagtt gtgcaactta ccattttcgc tgaagacttt cttctcgcca
    27781 aaggtttgct cgttatgcaa accgtctttt tctgtttgca caagcaaatt gccgccggcg
    27841 atgattcggc tgtcgctgtt gaagactgct ttgctatcaa taatcaaatc gctacctgca
    27901 atgattttag caggcgcagt tccggttact tgagtttctt gcgttacttt ttcataatcg
    27961 tatttatgcc aattttcatg cgccactcca tcaggggtgc gtaagtggtc tgattcattg
    28021 ttgtagacaa accagcctaa ttcatgttgc gtgccttctc gcaataattc gtgtcgtcca
    28081 aatgcttcgt aatcaacaat acgctcgcgc cctgtttcta ccaactgcgt tttcaaatgc
    28141 tcattggtat tgtgcagctt ttctacacct aaacgcattt tgcctgcagc ttcaatgatt
    28201 gcgccggcat tgtgtatcct ttgggctttg cctgtggctt ggtcattggt atctaatgcg
    28261 ccgccaaccg ccatatcgtt accgctgtaa atcagactgt tttcacggtt atttaattgt
    28321 tcaatgccta aattcaggtt ttcgcgtgcc gcaatggcgg cacctgtacc gttttcatct
    28381 tgattgtcta agcgggtagc cgcaatagcg atattgtcgc cataaatccg acctgtaccg
    28441 atattattca tttgcccggc ttggattttg gtttgttgtc cgtcaatcaa gcctctattg
    28501 gttaaattgt gctgcgtgcc aatgtctgtc gtaccgccgg attgaatgtt gccttgtact
    28561 gcattatcaa ggttatttgc tttaatccga atgcgttttc ctgcttgcaa ggtatgtgaa
    28621 tttttcaggc tgcctcgtgt actgagcgac aattcattgc ccgccacgat cttgcgttct
    28681 acataaaaat catcttgtaa cgcaatatcc agtttattat cagcggcaag tgtgccgttg
    28741 ttggataacg attttgcctg aatggcaaca tcacggcctg attgtatcgt gccattcgta
    28801 ttatcaatga caacggtaga ttgctgacca tcgtgaataa tcagttgttg attggtcgct
    28861 atttcgccat tttgattgtt caggctgcct gaaacggcta aatcctctgt tcctgccgac
    28921 aataatttgc cagtgttttg aatcgattga ctgtgaattt gagtgccttg ttgcgatacc
    28981 gccgtaccgc tgttttcaaa ggcttgactg cggatattga ctttgtgttc cgctgtatta
    29041 ttcatatctt gcacattggc ggcagccatc gtgccactat tgaccaaacg gccatttgca
    29101 tcaatcgcca cattaccgga agaagcaaac aactgccctt gattacgaat gcctgcttgt
    29161 tcggccgtac tgatcaaggt gattttgttg gcatacaaga atacgtgtga aatcggtatc
    29221 acgggcatcc aaaccgtgtc cggcgattac agcattgcct tgccttatct taaagccgct
    29281 aaagtctcct gcttgatatt gcggttggcc tgtcgtcaaa gtggcacggg aagcattgat
    29341 aaaaccacca ccattgactg caatccctgc cggattggca ataacgactt ctgcacgtcg
    29401 tccacccact tcaatatagc cattcagttg tgaaggatgg ctgctgttga tttggtttac
    29461 aaccacacgt gcttcgcccc ttgtcaacca aggattgcct tgaatccaac cgcctagctg
    29521 tgtttgggtg ttgctgcgac tgttgtttaa aatcgccccg cgattaccca catcaaactg
    29581 ggcatattga ttaacagaaa cccctgccga agtaggggtt tgaatattga cttgcggtat
    29641 gccgttacct gtttgcagaa tcgtggcttg ttgggtttta ggagcagctt tatcagtaat
    29701 aatgccgtca gcaaaagcaa tattgaccgt acccaaagcc aaacataaag aaaagcctaa
    29761 tgcagaaaaa caaaaggctt tggaatgagt aggaatgaaa gaaacggatt tcacataaac
    29821 gctgcccgaa ccactatcgg cacagctttt accttcgcgc ttggtggttt cggcaacagc
    29881 taccacagca ccgcgtttgc ggttgaaaat cacacgatag agggttttgt tcatgatttt
    29941 agttatttga tttttataga gttattacaa acattggaca gtctgggcat tctaggacaa
    30001 agattttggt aagtcaatta ccgccatttt tttattaaat taggggacgt accggataac
    30061 ggctaaaaat cccatttagg ttagaataag ccaatgagaa aagccgtcta agccggtata
    30121 aacaaaataa actcattggg ctatttgtcg caggcgtaac tgcaagaaca gcggcagagt
    30181 tggtaggcgt taataaaaat accgcagcct atgattttca tcgtttacga tgactgattt
    30241 atcaaaacgg tccgcattta gaaatgtttg atggcgaagt agaagcagat gaaagttatt
    30301 ttggcggaca acgcaaaggt aaacgcggtc gcggtgctgc cggaaaagtc gcagtattca
    30361 ggcttctcaa acgaaatggc aaggtttcct ttgccgtccg ttatgtacgt ttcgtaacat
    30421 tcgaagaatt cctgaaccct gcccgccgtt tcggtttccg tacccccctg ttagataagg
    30481 ggggaagatt cgaagcggtt gcc
//
LOCUS       DBJGGI010000020        29991 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.020, whole genome shotgun sequence.
ACCESSION   DBJGGI010000020 DBJGGI010000000
VERSION     DBJGGI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29991
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.020"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(70..1212)
                     /locus_tag="ACSIGJ_000882"
     CDS             complement(70..1212)
                     /locus_tag="ACSIGJ_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HHQ1329786.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSADAKKLSVSLWQKIKVGTN"
     gene            1515..1778
                     /gene="rpsT"
                     /locus_tag="ACSIGJ_000883"
     CDS             1515..1778
                     /gene="rpsT"
                     /locus_tag="ACSIGJ_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HHQ1329787.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            1938..3086
                     /locus_tag="ACSIGJ_000884"
     CDS             1938..3086
                     /locus_tag="ACSIGJ_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HHQ1329788.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPTASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPFWYNNSPNYAPSSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAENLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDWDGI"
     gene            3228..3929
                     /locus_tag="ACSIGJ_000885"
     CDS             3228..3929
                     /locus_tag="ACSIGJ_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HHQ1329789.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPQ
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPPEKAELPEQQ"
     gene            3987..5795
                     /gene="aspS"
                     /locus_tag="ACSIGJ_000886"
     CDS             3987..5795
                     /gene="aspS"
                     /locus_tag="ACSIGJ_000886"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HHQ1329790.1"
                     /translation="MRTNYCGLISEQYLGQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLGITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVATGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALNVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDAGNLSNGEDSGLQSPIVKFLSENALKEIIERTAAQ
                     NGDIIFFGADKAKVVNEAVGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTDAPNSVDDKQLRELSLRLRQKAVETKEA"
     gene            5945..6229
                     /locus_tag="ACSIGJ_000887"
     CDS             5945..6229
                     /locus_tag="ACSIGJ_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HHQ1329791.1"
                     /translation="MKKLLLAAVVSLNAATAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVYDVDADDYWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            6396..8288
                     /gene="speA"
                     /locus_tag="ACSIGJ_000888"
     CDS             6396..8288
                     /gene="speA"
                     /locus_tag="ACSIGJ_000888"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HHQ1329792.1"
                     /translation="MPILTIREVCNINHWSIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPFLGFFMVGAYQEILGNMHNLFGDTATADVVVREDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            8387..9310
                     /gene="speB"
                     /locus_tag="ACSIGJ_000889"
     CDS             8387..9310
                     /gene="speB"
                     /locus_tag="ACSIGJ_000889"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HHQ1329793.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSFGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            9508..10974
                     /locus_tag="ACSIGJ_000890"
     CDS             9508..10974
                     /locus_tag="ACSIGJ_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HHQ1329794.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKVMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLSEMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFLAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFVMSVVNFLIFSVIGSIWWKVLGYW"
     gene            complement(11178..12254)
                     /locus_tag="ACSIGJ_000891"
     CDS             complement(11178..12254)
                     /locus_tag="ACSIGJ_000891"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HHQ1329795.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWHIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGIRSHGCVRMKS
                     PDALEFAKTIATGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDTASLKKSIGQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEVSETRTPEVPDVHTPEAQAHLNTQSDGTPTAYTEPAADSSPQVESPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            12318..12455
                     /locus_tag="ACSIGJ_000892"
     CDS             12318..12455
                     /locus_tag="ACSIGJ_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HHQ1329796.1"
                     /translation="MATETNIAEISALDYEGRGVAKVGGKTIFIKGALLDCLMLGCFR
                     L"
     gene            12477..13619
                     /gene="bioF"
                     /locus_tag="ACSIGJ_000893"
     CDS             12477..13619
                     /gene="bioF"
                     /locus_tag="ACSIGJ_000893"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HHQ1329797.1"
                     /translation="MKVFRQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSETCIVPYILGGNEATLAK
                     AEYLQGQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAACL"
     gene            13629..14276
                     /locus_tag="ACSIGJ_000894"
     CDS             13629..14276
                     /locus_tag="ACSIGJ_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl esterase BioG family
                     protein"
                     /protein_id="HHQ1329798.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGMPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRSKFERRICGDKASFEDYQQFPARPFDEIHQELTALFAMIGQDRR
                     TDLIRWTNALFGSGDKIFIPANQHRYWTPRCTVQETDGGHYLFSRFTHWSALWNH"
     gene            14264..15046
                     /gene="bioC"
                     /locus_tag="ACSIGJ_000895"
     CDS             14264..15046
                     /gene="bioC"
                     /locus_tag="ACSIGJ_000895"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HHQ1329799.1"
                     /translation="MESLTAINKPRIRQAFQKALSDYDRHALIQQKMTINLIAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVRSRLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKTNGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQAWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFGMPSGKVRLTYHPLFFIARHSAAGRQ"
     gene            15288..16725
                     /locus_tag="ACSIGJ_000896"
                     /pseudo
     CDS             15288..16725
                     /locus_tag="ACSIGJ_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            16728..17826
                     /locus_tag="ACSIGJ_000897"
                     /pseudo
     CDS             16728..17826
                     /locus_tag="ACSIGJ_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            17838..18373
                     /locus_tag="ACSIGJ_000898"
                     /pseudo
     CDS             17838..18373
                     /locus_tag="ACSIGJ_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            18375..19017
                     /locus_tag="ACSIGJ_000899"
                     /pseudo
     CDS             18375..19017
                     /locus_tag="ACSIGJ_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
     gene            19038..19397
                     /locus_tag="ACSIGJ_000900"
     CDS             19038..19397
                     /locus_tag="ACSIGJ_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HHQ1329800.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSVYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            19489..19564
                     /locus_tag="ACSIGJ_000901"
     tRNA            19489..19564
                     /locus_tag="ACSIGJ_000901"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19522..19524,aa:Asn,seq:gtt)
     gene            complement(20062..20973)
                     /gene="crgA"
                     /locus_tag="ACSIGJ_000902"
     CDS             complement(20062..20973)
                     /gene="crgA"
                     /locus_tag="ACSIGJ_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HHQ1329801.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLEMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLNLTEEGAQYFRRAQRILQEMAAAETEMLAVHEVPQGVLRVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSHFRVVASPEYLAKHGTPQSAEDLANHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSSCGIACLSDFLVDNDITEGKLIPLFAEQTSNKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVKELGKNMNRTNTK"
     gene            21212..21790
                     /locus_tag="ACSIGJ_000903"
     CDS             21212..21790
                     /locus_tag="ACSIGJ_000903"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HHQ1329802.1"
                     /translation="MNILLLDGGKAFGHSHGGLNHTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYMDEVFTGGHGKLYQSDGRHRVN
                     PTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFIGLS
                     RLPTFLCNDVVKNPQVEKYLADYQAHLEKVFG"
     gene            complement(21998..23383)
                     /gene="pntB"
                     /locus_tag="ACSIGJ_000904"
     CDS             complement(21998..23383)
                     /gene="pntB"
                     /locus_tag="ACSIGJ_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HHQ1329803.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKRGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIHKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLAAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     ISSSGSQEIEEYREVKAADVAEMLKGASSVIITPGYGMAVAQAQYPVAEITELLRKNG
                     IEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDGILAELKK"
     gene            complement(23463..23846)
                     /locus_tag="ACSIGJ_000905"
     CDS             complement(23463..23846)
                     /locus_tag="ACSIGJ_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HHQ1329804.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRGFLAHTQ
                     GAAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(23850..25388)
                     /locus_tag="ACSIGJ_000906"
     CDS             complement(23850..25388)
                     /locus_tag="ACSIGJ_000906"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HHQ1329805.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLKEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFLQ
                     ESGGSGDGCAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVNL
                     TKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSARPQQTPSEKAAPAAK
                     PEPKPVPLWKKLAPAAIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYHVVWNVSHSL
                     HTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIAGINIFGGFAVTRRMLNM
                     FKKG"
     gene            25677..26510
                     /gene="serB"
                     /locus_tag="ACSIGJ_000907"
     CDS             25677..26510
                     /gene="serB"
                     /locus_tag="ACSIGJ_000907"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HHQ1329806.1"
                     /translation="MPQALVLQFPSAEALPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEKDAALLDSRQIDHAVLPNMAFGELGLIVSDMDSTLITIECIDEIAAGVGLKGKVAE
                     ITERAMRGELDFEQSLRSRVALLAGLDEQILADIYENVLKLSPGAEFLLDECKRHNVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANILEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPILKEAGIGVVYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            26574..27797
                     /locus_tag="ACSIGJ_000908"
     CDS             26574..27797
                     /locus_tag="ACSIGJ_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HHQ1329807.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIRHTAYGYDADGAY
                     ISFREGVAQAFGMRRVAMLTLCGAIAGGGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEGEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGATAAFFRRTAQKFPFIKRDNIKIIPLAVCMFALIGVIAVWFP
                     EILGNGKAGNQLTFGGLTGWQYSLELTAVKWAVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSESAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            27958..29538
                     /gene="purH"
                     /locus_tag="ACSIGJ_000909"
     CDS             27958..29538
                     /gene="purH"
                     /locus_tag="ACSIGJ_000909"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HHQ1329808.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQNLHKLGVEILSTGGTAKLLAGA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPGCTLEDAIENIDIGGPAMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     AGNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGQPQIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYNQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLKAGANRFELKRVGGGLLVQTPDINR
                     INRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMAVTGIRHFRH"
BASE COUNT         7335 a         7315 c         7853 g         7488 t
ORIGIN      
        1 atatttctat ttcaatccaa tataaaatgc cgtccgaaca ttgttcggac ggcattttaa
       61 tgcttcaaat cagttggtgc cgactttgat tttctgccac aggctgaccg acagtttttt
      121 tgcatccgcg ctcatttgcg gcatcacgaa accgtctttc atatcctgct cgttcgggaa
      181 gatggaacgg gtgttcacca gctcggcagg cattttttcg cgcgccggtt tgctggcggg
      241 ggcaaaggtt acggcgatgc cgtttttcgc cgcgatttcg gggtcgagcg tgtagttgat
      301 gtatttgtgg gcattggcga cgtttttcgc atcggcggga atcagccaag actcaatcca
      361 aaagcccatg cctttcggat tcaacacttc gatgccgacg ttgtttttca cttcttcgga
      421 acgtgctttc gccaagttca aatcgccgcc gttgcctgcc gccaggcaga tgtcgccgcg
      481 cgccagctcg tcgatgatgg acgggctgaa acgtttgaca tccggacgga tagacttcaa
      541 cacttccgcc gctgccttca aatcttcagg attcgaacct ttggggtctt tgcccaagta
      601 gttcagcaaa atcgggaaca tttcgcttgg ggtgtcccac agggcgatgc cgcaggattt
      661 cagcttgtgg gtgtattcgg gtttgaacag caaatcccag ccgttttcgg gcagcttgcc
      721 gcccaaaagc tctttgcctt tcgccgtaat cgcaatcgtg ttcacgccgg agaaataggg
      781 gacggcatac tggttgcccg ggtcggcggt ttccagcatt ttcaagagtt cgggatcgat
      841 gtttttatag ttgggaatca ggtctttgtt gactttttga tacgcgcccg cctcgatttg
      901 gcgcggcagg aaggcgatgc ccggcacaac caaatcgtaa ccggatttgc cggttagcat
      961 tttggcttcc agcgtttcat tgttttcgta caagtcgtaa gtcagcttca gattgttggc
     1021 ttttttaaag tcttcgaccg tactctcgtc aacatagttc gaccagttgt agatgttcag
     1081 agtatcggtg gcagcggctt cggcattggc agcagacgca gcgtctgctt gaggttgcac
     1141 ggcgtttttt tcgctgccgc cgcaggctgc cagagacagc gcggccaaaa cggctaatac
     1201 ggattttttc atacgggcag attcctgatg aaagaggttg gaaaaaaaga aaaccccgcg
     1261 ccccataaac accccggcgc aaggtttggg cattgtaaag taaatttgtg caaactcaaa
     1321 gtgatattgg actgatttcc ctaaaaaatt accctgtttt caaaagaggg ggaaaacgtc
     1381 cgcacgattt tgccgttttt ttgcgctgtc agggtgtccg acgggcggat agagagaaaa
     1441 ggcttgcata taatgtaaac cccctttaaa attgcgcgtt tacagaattt atttttcttc
     1501 caggagattc caatatggca aacagcgcac aagcacgcaa acgtgcccgc cagtccgtca
     1561 aacaacgcgc ccacaacgct agcctgcgta ccgcattccg cactgcagtg aaaaaagtat
     1621 tgaaagcagt cgaagcaggc gataaagctg ccgcacaagc ggtttaccaa gagtccgtca
     1681 aagtcatcga ccgcatcgcc gacaagggcg tgttccacaa aaacaaagcg gcacgccaca
     1741 aaagccgtct gtctgcaaaa gtaaaagcac tggcttgatt tttgcaaaac cgccaaggcg
     1801 gttgatacgc gataagcgga aaaccctgaa gcccgacggt ttcggggttt tctgtatttc
     1861 gggggtaaag ttcgaaatgg cggaaagggt gcggtttttt atccgaatcc gctataaaat
     1921 gccgtttgaa aaccaatatg ccgacaatgg gggcggagat gaatacacga aatatgcgct
     1981 atattctttt gacaggactg ttgccgacgg catccgcttt tggagagacc gcgctgcaat
     2041 gcgccgcttt gacggacaat gttacgcgtt tggcgtgtta cgacaggatt tttgcggcac
     2101 agcttccgtc ttcggcaggg caggaagggc aggagtcgaa agccgtactc aatctgacgg
     2161 aaaccgtccg cagcagcttg gataagggcg aggcggtcat tgttgttgaa aaaggcgggg
     2221 atgcgcttcc tgccgacagt gcgggcgaaa ccgccgatat ctatacgcct ttgagcctga
     2281 tgtacgactt ggacaaaaac gatttgcgcg ggctgttggg cgtacgcgaa cacaatccga
     2341 tgtaccttat gccgttttgg tataacaatt cgcccaacta tgccccgagt tcgccgacgc
     2401 gcggtacgac tgtacaggaa aaattcggac agcagaaacg tgcggaaacc aaattgcagg
     2461 tttcgttcaa aagcaaaatt gccgaaaatt tgtttaaaac ccgcgcggat ctgtggttcg
     2521 gctacaccca aagatccgat tggcagattt acaaccaagg caggaaatcc gcgccgttcc
     2581 gcaatacgga ttacaaacct gaaattttcc tgacccagcc tgtgaaggcg gatttgccgt
     2641 tcggcggcag gctgcgtatg ctcggtgcgg gttttgtcca ccagtccaac ggacagagcc
     2701 gtcccgaatc gcgttcgtgg aacaggattt atgccatggc aggcatggaa tggggcaaat
     2761 tgacggtgat tccgcgcgtg tgggtgcgtg cgttcgatca gagcggcgat aaaaacgaca
     2821 atcccgatat tgccgactat atggggtatg gcgacgtgaa gctgcagtac cgcctgaacg
     2881 acaggcagaa tgtgtattcc gtattgcgct acaaccccaa aacgggctac ggcgcgattg
     2941 aagccgccta cacgtttccg attaagggca aactcaaagg cgtggtacgc ggattccacg
     3001 gttacggcga gagcctgatc gactacaacc acaagcagaa cggtatcggt atcgggttga
     3061 tgttcaacga ctgggacggc atctgaatac cgtttcaaac ggcatatcaa gttgccgtgc
     3121 cgtctgaagc cgccggcggt ttggcggggc gggataaaaa attcgtttga atccgggcaa
     3181 acgcgtataa tgtgcggttt gtacggactt tgtcggaatc aagcaagatg acggaacctg
     3241 cggccgaagg cggcaaagct gccaaggcgt taaaaaaata tctgattaca ggcattttgg
     3301 tctggctgcc gattgcggta acggtttggg tggtttccta tatcgtttcc gcgtccgacc
     3361 agcttgtcaa cctgctgccg aagcaatggc ggccgcaata tgttttgggg tttaatatcc
     3421 ccgggctcgg cgttattgtt gccattgccg tattgtttgt aaccgggtta tttgccgcaa
     3481 acgtgttggg ccggcagatt cttgccgcgt gggacagcct gttgggtcgg attccggttg
     3541 tcaaatccat ctattcgagt gtgaaaaaag tatccgaatc gctgctgtcc gacagcagcc
     3601 gttcgtttaa aacgccggta ctcgtgccgt ttccccaatc gggtatttgg acaatcgcat
     3661 tcgtgtccgg tcaggtgtcg aatgcggtta aggccgcatt gccgcaggat ggcgattatc
     3721 tttccgtgta tgtcccgacc acgcccaacc cgaccggcgg ttactatatt atggtaaaga
     3781 aaagcgatgt gcgcgaactc gatatgagcg tggacgaagc gttgaaatat gtgatttcgc
     3841 tgggtatggt catccctgac gacctgcccg tcaaaacatt ggcaggacct atgccgcctg
     3901 aaaaggcgga gttgcccgaa caacaataaa gccgccgttc agacggcatt ttccgttttc
     3961 agtttaaatc aataaaaggt gattttatgc gtaccaacta ttgcggcctg atcagcgagc
     4021 aatacttagg ccaaaccgtt accgtcaaag gctgggtaca ccgccggcgc gaccacggcg
     4081 gtgtgatttt tatcgacctg cgcgaccgcg aaggcatcgt ccaagtcgtg atcgatcccg
     4141 acacgcccga agcgtttgcc gctgccgatt ccgcccgtaa cgaatacgtt ttgggcatta
     4201 ccggccgcgt acgcaaccgt cccgaaggca cgaccaacga taaaatgatt tccggcaaaa
     4261 tcgagattct tgccaaagaa atcgaagtgt tgaacgccgc cgctacgccg ccgttccaaa
     4321 tcgacgacga aaacatcagc gaaaacgtgc gcctgaccaa ccgcgttatc gacctgcgcc
     4381 gccctgtgat gcagcgcaac ctgcgcctgc gttaccaagt cgctacgggc gttcgccgtt
     4441 atctggacgc gcaaggcttc atcgacatcg aaacgccgat gctgacccgc tccactccgg
     4501 aaggcgcgcg cgactacctc gtaccgagcc gcgttcatcc gggcgagttt ttcgcgctgc
     4561 cgcaatcgcc gcaattattc aaacaactgt tgatggtggc gggtttcgac cgttactacc
     4621 aaatcaccaa gtgcttccgt gacgaagact tgcgcgccga ccgccaacct gaattcactc
     4681 aaatcgactt ggaaacctcg ttcctgaacg aggatgaaat catggatatc actgaaggca
     4741 tggccaaaca agtcttcaaa gatgctttga acgtagattt gggcgacttc ccacgcatgc
     4801 cttattctga agccatgttc tactacggct ctgacaaacc ggacatgcgc atcaacttaa
     4861 aattcaccga gttgaccgac ctgatgaaaa cggaagaatt caaagtcttc cgtggcgcag
     4921 ccgacatgaa aggcggccgc gtagtcgctc tgcgcgtgcc taacggcgcc aaattcagcc
     4981 gcaaagaaat cgacgaatac actaaatttg tcggcatcta cggcgcgaaa ggtctggcat
     5041 acatcaaagt aaacgatgcg ggcaacctct ccaacggcga agacagcggc ctgcaatctc
     5101 caatcgtaaa attcttgtct gaaaacgccc tgaaagaaat catcgagcgt accgccgcgc
     5161 aaaacggcga catcatcttc ttcggtgcgg acaaagccaa agtcgtgaac gaagccgtcg
     5221 gcgcattgcg tatcaaagtc ggcttggaac acggcaaaga caacggctac ttcacagacg
     5281 aatggaaacc tttgtgggtt gtcgatttcc cgatgttcga atacgacgaa gaagccgacc
     5341 gctacgttgc cgtacaccat ccgtttaccg ctcctaaaga aggtcatgaa gacctgatgg
     5401 tttccgatcc tgcaaactgc ctggcccgtg cctacgatat ggtattgaac ggctgggaaa
     5461 tcggcggagg ctctatccgt atccaccgtg cagacgtaca agagaaagtg tttgccgcgc
     5521 tgaaaatcag ccctgaagag caacaagaga aattcggctt cctcttggac aacctgaaat
     5581 tcggtgcgcc tcctcacggc ggccttgcat tcggcctcga ccgtctggta acgctgatga
     5641 ccggtgccga atccatccgc gacgtgattg ccttcccgaa aacacaacgc gcccaatgcc
     5701 tgctgaccga tgcgcccaac agcgtggacg ataagcagtt gcgtgaattg agcctgcgtc
     5761 tgcgtcaaaa agcagttgaa acaaaagaag cataaggaaa acagagccgt ttgacggctc
     5821 tgttttttca gacggcattt acgcttcttg acttccctct aattcaaacc taattttgct
     5881 gtgttttaat ggcggtattg aaaaacacat cttgttcaat caaccgataa aaaaaggact
     5941 gaaaatgaaa aaactgttat tggccgccgt tgtttcccta aatgccgcaa ccgcatttgc
     6001 cggcgactct gccgagcgtc agatttacgg cgatccccat tttgaacaaa accgcacaaa
     6061 agccgtgaaa atgttggaac agcgcggtta tcaggtttac gatgtcgatg ccgacgacta
     6121 ctggggcaaa cctgttttgg aagtggaagc ctataaagac ggccgcgaat acgacatcgt
     6181 gttgtcttac cccgacctga aaatcatcaa agagcagctc gatcgctgac tcctttgatg
     6241 gaaagatgaa ccaaaatgcc gtctgaagcg ttcagacggc attttgcctg ttcctcatca
     6301 ggtatgaggc aggcttttct tattaaaaaa atgacatttc acgctgattt gttataatca
     6361 ttccttttca acacgacaga cggagcaggt ttattatgcc tatccttacc atccgtgaag
     6421 tgtgcaacat taatcattgg agcataggtt attatgatgt tgacgattcc ggcgaaatca
     6481 tcgtccgccc caatccctcg caacacaatc aaactgtttc actgcaaaaa ctgactgaag
     6541 ccgtgcaaca aaaacatcag gcgcgcctgc ctgttttgtt ttgttttccg caaatcctcg
     6601 aacaccgcct ccgcgacatt aaccgcgcct ttcagacggc acgggaagag tgcggctata
     6661 agggcggtta ttgtttggtt taccctatca aggtcaacca acaccgccgc gtcatcgagt
     6721 cgcttatgtc aagcggacaa ccgcatggtt tggaagccgg ttctaaagcc gaactgatgg
     6781 cggttttggc acacgccggc acccggcaaa cattaatcgt ctgcaacggc tataaagacc
     6841 gtgaatatat ccgtttcgcc ttgatgggcg aaaaactggg gcatcaggtt tatttggtga
     6901 ttgagaagct gtccgaaata caaatggtat tggaagaggc ggaaaaactc ggcatcaagc
     6961 cccgtttggg tgtgcgcgcc agactggctt cccaaggttc gggaaaatgg cagtcttcgg
     7021 gtggggaaaa atcaaaattc ggcttgtcgg cttcccaagt tttgcaactg gtcgatattt
     7081 tgaaacaaaa aaacaggctg gactgcctgc agcttttgca tttccatttg ggctcgcagc
     7141 ttgggaacat ccgtgatgtt gccacaggtg tacacgaatc ggctcggttt tatgttgagt
     7201 tgcacaaact gggggtaaat atccgctgtt ttgatgtagg tggcgggctt ggcgtggatt
     7261 acgaaggaaa ccgcacacaa tcggattgtt ccgttaatta cagcctgaac gaatatgccg
     7321 ccacagtcgt atggggcatc agtcaggctt gtctcgaaca cgggctgccg catccgacaa
     7381 tcattaccga gagcgggcgc ggcattaccg cacatcacgc cgttttggtt gctaatgtta
     7441 taggcgttga acgttacaaa ccgcgtcggc tggatgcgcc atcgcccgaa gcaccgcgtg
     7501 tgttgcacag tatgtgggaa acttggacgg atatttccgc ctcgcgggaa aaacgttcct
     7561 tacgcagctg gatacacgaa gggcagtttg atcttgccga tgtgcataat cagtataatg
     7621 tcgggctgtt gagtttggcg caacgtgcgt gggcggagca actgtattta aatatctgtc
     7681 atgaagtcgg cgaattgttt aatgaaaaac accggtctca ccgaaccatt attgacgaat
     7741 tgcaagaacg ttttgccgat aagctgtatg tcaatttctc actcttccaa tctttgcccg
     7801 atgcttgggg catagatcaa cttttccctg tttgtcctat taccggtttg aatgaaccga
     7861 ttgcgcgccg cgccgtgttg ttggacatta cctgcgattc agacggtacg attgaccact
     7921 acatcgacgg cgacggcatc gccggtacga tgcctatgcc tgattatccc gaagaagagc
     7981 cgccgttttt aggctttttt atggtgggag catatcagga aatactcggc aatatgcaca
     8041 atcttttcgg cgacactgcc actgctgatg ttgttgtaag ggaagacgga caatttaccg
     8101 tcatcgatta cgatgaagga aacaccgttg ccgatatgct cgaatacgtt tatcaagatc
     8161 cgaaagagct gatgaaacgc tatcgcgaac aaatcgaaca ttcagacctt cctgcctcgc
     8221 aggctatgtc tttcttaaaa gaactcgaag cggggcttaa tggttatacc tatttggaag
     8281 acgaatagac gcatcaaggt atcggatatg tcgtctgaag cccgattttc ttactcaaac
     8341 accaatcatc acgaccgatt gaaaccaatt acaaggaatc attacgatgc aatacagcac
     8401 actggcagga caaaccgaca actccctcgt ttccaataat ttcgggtttt tgcgcctgcc
     8461 gcttaatttt atgccgtatg aaagccatgc cgattgggtt attaccggcg tgccttatga
     8521 tatggcggtt tcagggcgtt ccggcgcgcg tttcggtcct gaagccatcc ggcgcgcctc
     8581 cgtcaacctc gcttgggagc accgcaggtt tccgtggaca tttgatgtgc gcgaacgcct
     8641 gaacattatt gattgcggcg acttggtttt ttcttttggc gacagcaggg attttgtcga
     8701 aaaaatggaa gcgcacgccg gcaaattact ttctttcggc aaacgctgtt tgagtttggg
     8761 cggcgaccat ttcattaccc tcccgttgtt gcgcgcccac gcccgctatt tcggcaaact
     8821 cgcactgatt cattttgacg cgcacaccga cacctacgac aacggcagcg aatacgacca
     8881 cggcacgatg ttttataccg cccccaagga aggcctcatc gacccgtccc gttccgtaca
     8941 aatcggcata cgcaccgaac acagtaaaaa attgcctttt actgtgttgt ccgcccccaa
     9001 agtcaatgaa gacagtgttg aagagaccgt ccgtaaaatc aaagaaaccg tcggcaatat
     9061 gcccgtttac ctgactttcg acatagactg tctcgacccg tcgttcgccc ccgggaccgg
     9121 tacgcccgta tgcggcggct tgagcagcga cagggcatta aaaatcctac gtgggctgac
     9181 ggatctcgac atcgtcggta tggatgttgt agaagttgcc ccctcttacg accaatccga
     9241 cattaccgct ttggccggcg ccacaattgc cttggaaatg ctttaccttc aaggtgcgaa
     9301 aaaggactga acgtccggca tcccccgggt tttcgccgtg ccgttcaaac ggcgtattca
     9361 gtctaatgaa aattcaaata ctgaaacaaa agttgcccgg agccgcatat cggaaagacg
     9421 gtgaaatatc gtaatataac ttataaaaca attagttaaa tattattttt ccgatttttc
     9481 gggacggtct tttttacgga ggtcaatatg atgaaattgg gtttcaaacc gatacccctc
     9541 gccattgccg cagtattgtg cgccctggtt ttggcactgc ccgtacccga cggggtcaag
     9601 cctcaggctt ggacgctgct ggctatgttt gtcggtgtga ttgccgccat tatcggcaag
     9661 gttatgccgt tgggcgcgct gtcgattatt gccgtcgggt tggtcgcagt aaccggcgta
     9721 accgccgaca aaccgggcgc ggcgatgagc gatgcgttga gtgcgttcgc caatccgttg
     9781 atttggctga ttgccatcgc agttatgatt tcgcgcggtt tgctcaaaac agggctgggg
     9841 atgcgtatcg gatatttgtt tatcgccgtt tttggaagaa aaacgctggg catcggttac
     9901 agtctcgctc tttccgaact gctgctggct cccgttaccc cttccaatac cgcgcgcggc
     9961 ggcggcatta tacatccgat tatgcagtcg attgccggca gttacggctc caatcccgca
    10021 aaaggcacag aaggcaagat gggtaaatat ttggctttgg tcaattatca ttccaatccc
    10081 atttcgtcgg ctatgtttat tactgcaact gcccccaacc ctttaatcgt caacttgatt
    10141 gccgaaaatt taggcagtag tttccgtctt tcttgggggg cgtgggcgtg ggcaatggct
    10201 gttcccggcg ttatcgcctt tttcgttatg cctttgattt tatatttttt gtatccgcct
    10261 gaaattaaag aaacgcccaa tgctgttcaa tttgccaaag accgtctgag cgagatgggt
    10321 aaaatgtcgg cagacgaaat cattatggcg gtcattttcg gtatcttgct gctgttgtgg
    10381 gcagatgttc ccgcccttat taccggcaat cacgctttta gtatcaacgc caccgccacc
    10441 gcatttatcg gattaagcct gcttttgctt tccggtgtat tgacttggga cgatgttttg
    10501 aaagaaaaaa gcgcgtggga tacgattatt tggtttggcg cattgattat gatggccgca
    10561 tttttaaata aactcggact gattaaatgg ttctccggag tgttggcgga aagtgtcggc
    10621 ggtttgggcg ttagcggcac ggctgcgggc gtaatcctcg tgcttgctta tatgtatgcg
    10681 cattatatgt ttgccagtac tactgcacat attaccgcta tgttcggcgc atttctcgct
    10741 gctgccgttt cactgaatgc cccggcgatg ccgaccgcgc tgatgatggc ggccgcatcc
    10801 aacattatga tgaccctcac tcattatgcg accggtactt cacctgtgat tttcggctcg
    10861 ggctacacca caatgggaga atggtggaag gcgggttttg tcatgagcgt agtcaatttt
    10921 ctgatttttt ccgttatcgg cagcatttgg tggaaagttc tgggatattg gtaaagggaa
    10981 aaataaattt ccaatctgtg tttatttgat tgggcgacta ttatcgtgaa atatgccgtc
    11041 taaagccttc agacggcata tttgtgcgct tggatgttgc agaaagcggc agggcggcgg
    11101 tgtaggaaaa gccaaacaaa aaccaaaccg cctgtcaact tctgataaac ataagtatta
    11161 aataatcaga aggttattca attacctaaa cgcaaattcc cctgccgtat cacatctatt
    11221 gaaaataata catcaaccgg ctcggaagca gcctgatcag gtgattctac ttgcggcgat
    11281 gaatcggcag ccggttcggt ataggcagtc ggcgtgccgt cggattgggt atttaaatgc
    11341 gcttgtgcct cgggcgtgtg tacatcaggc acttcgggcg tgcgtgtctc ggatacttcg
    11401 gcagagttgg tttcctcagt ttgttcaatg acttcagctt ggctgtatga ggaagaaccc
    11461 tgtatccacg ccagcgattt gagcggcatc ttcatcttgc cgtttttgcc gcaggtcaga
    11521 cagacggccg atccggtgcg gtctttgagt acggcatcga ctttctcggg cgcgatggtt
    11581 ttgccctgtg tttttgccca ttgcccgatg ctttttttca gggaggcggt gtcaaggttg
    11641 ttcttaccgt aagggtcgcg gaaggcggca agccacaggt tgcgatccgc atcttcattg
    11701 aggcccgcca tttgatagat gacggaggcg ggcgagccgg tggcgatggt tttggcaaac
    11761 tcgagcgcgt cgggcgattt catgcggacg cagccgtgac tccggatgcc cgggacgctg
    11821 gccggtgcat tggttccgtg tatgcccaaa ccgagtttcg ggtctcctaa gcggacaaaa
    11881 accggcccca aagggttgtc cgggccggcg gctatggttt ttacgccgtc gccgcgttct
    11941 ttctgtatgg attttgggat gtgccaaaca gggttatagg ctttcgcccc gattttatgt
    12001 tcgcctagat tggtttgtgt catcgcccga cctactgcaa cgggataaac cttggtcagt
    12061 ttgccgtcgg tgtagaggaa caggcgttgc tgagggatgt taatgaagac atgttgacct
    12121 tgtgcgacgg gggagacatc gggaatgacg gtgtttgcgt atgaaaaacc gcttatcaat
    12181 agtgcggcag tgcggcagat tgttttattc atatcaaaat atggtgtgtg tccgataggt
    12241 tttcggcaaa tcatacctga aaccgtacca atttgtgcga aaatatgcgc ttcggtacag
    12301 tgcggacgga ttgggtaatg gcaacggaaa caaatatcgc ggaaatttcc gccttggatt
    12361 atgaaggcag gggtgtggca aaggtcggcg gcaaaacgat tttcattaaa ggggcattac
    12421 ttgattgttt gatgctgggt tgcttcaggc tttaactcag gaatatttac atcataatga
    12481 aagtttttag acaacagctt gaacaactcg gcgcgcaaaa tcaatatcgt tcgattccgg
    12541 atttgattca tcaagggcgg tatattacgc gggaaaaccg caaaatgctg aatatgtcgt
    12601 ctaatgatta tttggggttg gcatcagatg aaaacttacg ccggtctttt ttgcagcaat
    12661 acggcggtaa ttttccctct tttaccagtt cttcatcacg tttattaacg ggcaactttc
    12721 ctatttatac cgatttggaa gagcttgtcg cacaacgttt ccaacgggaa agcgcgttat
    12781 tgttcaacag cggctatcac gccaatctcg gtattttgcc tgctttgacg acgacgaaaa
    12841 gtttgatttt ggcagataaa ttcgttcacg ccagtatgat tgacggcatc cgtttgagcc
    12901 ggtgtgcgtt tttccgttat cgtcataatg attatgaaca tttgaaaaat ctgcttgaaa
    12961 aaaacgtcgg aaaatttgac cgcactttta tcgttaccga atctgttttc agtatggacg
    13021 gcgatgtggc ggatttgaaa cagcttgtcc aattaaaaaa acagtttccc aatacttatc
    13081 tttatgtgga tgaagcccac gcaatcggtg tttatgggca aaacggattg gggattgccg
    13141 aacgggataa tctgattgcc gagattgatt tattggtcgg cactttcggt aaagccttag
    13201 cctcggtggg ggcgtatgcc gtctgcaacc aagtattgaa agaatgtttg attaatcaaa
    13261 tgcgcccatt gattttttca accgcattgc cgccgtttaa tgtggcttgg acttatttta
    13321 tttttgaacg attgccgcaa ttctcaaaag aaagaagtca tcttgagcag ttaagcgcat
    13381 ttttacggcg ggaagtggcg catcggacgc aaataatgcc gagcgaaacc tgtatcgtcc
    13441 cctatatttt aggcgggaat gaagccaccc ttgccaaagc ggaatatctg caaggtcagg
    13501 gttattattg cctgcccatc agaccgccga cagtaccaaa aaacacatcc agaatccgcc
    13561 tgtctttaac tgccgatatg acaacggatg aagttcggca gtttgcggcg tgcctgtaag
    13621 gatatgatat gaaaacaaaa ttttacaatc atcaaggcga acatttaatc ctgtattttg
    13681 caggttgggg aatgccgccc gatgctgtaa atcatttgat tttgccggaa aatcacgatt
    13741 tattgatttg ctatgattat caagatttaa atttggattt tgatttttcc gcctatcggc
    13801 acatccgttt ggtggcgtgg tcaatgggcg tttgggcggc agagagggca ttgcaaggaa
    13861 taagattaaa atccgcaacg gcagtgaatg gcacaggttt gccttgcgat gataatttcg
    13921 gtatcccttg cgccgttttt aaaggcacat tggagaatct cacagaaaat actcgttcaa
    13981 agtttgaacg tagaatctgt ggcgataaag catcttttga agattaccaa caatttcccg
    14041 cccgtccgtt tgacgaaatt catcaagaac ttaccgcact ttttgcgatg atcgggcaag
    14101 ataggcgtac agatcttatc cgctggacaa acgccttgtt cggatcgggc gataaaattt
    14161 ttattcctgc caatcagcac cgatattgga caccgcgttg caccgttcaa gaaacagacg
    14221 gcgggcatta cctgttttca agatttaccc attggtcggc actatggaat cactgactgc
    14281 cataaataaa ccgcgcattc ggcaggcttt ccaaaaagca ttaagcgatt atgacaggca
    14341 cgccttaatc caacaaaaaa tgacgattaa tctaatcgcg catttgcaag attatttgcc
    14401 ggatatgcca ttggaaaacg tgttggaatt gggctgcggc tcaggaatgt tgagtgcctt
    14461 gctgcaaaaa cagatttcag cgaattattg gttatttaat gatttgtgcg atgtacgaag
    14521 ccgactggct gaaaaattgc ctcaatcctt tgatttttat tgcggcgatg cggaaaactt
    14581 tccttttcaa cgacaatttg acttaatcgc aagcgcatct gccgtgcaat ggtttcacca
    14641 acccgacgct tttatcgccc attgcaaaac aggcttgaaa acaaacggat tattggcggt
    14701 tgcgaccttt ggcaaagaca atttaaaaga agtccgccaa atcacaaata taggcttaaa
    14761 ttacccgact ttatcccaat ggcaggcttg gttagccaaa gattttgagc ttttatggtg
    14821 tgaggatttt aaggtaatac tagactttga tacgccgtta gatgtactca aacaccttaa
    14881 atatacaggc gttaccgcca cgaaccaaaa aaattggaca agaaaaaatc tcaatggatt
    14941 tattggcgat tacttgtcgg cgttcggtat gccgtcgggc aaagtgcgcc tgacttatca
    15001 tccgttattt tttatcgcgc gccactctgc ggcgggcagg caataaggca gcttatgggc
    15061 aaagttattt ttatatcggg tattgatact gatgtgggta aaaggtaata tggcgagcct
    15121 tatgcagaag gcatattgtc aaacgttaaa ttatggtatg atttaaaatt tacaagtcta
    15181 tttcagtaaa tcgttaataa taaaagcgga caatttaata ataaaagcgg ataattggcc
    15241 gttgcaggcg gtcagctatc cgctcgttac ggtatcgggg gtttcctttg gaaaactcat
    15301 taattatcgg tctggcactg gcggtattgc tgatgctttt ggttatgcgt gcgaagcagg
    15361 gtaagaaaac accgaagcgc aaatcccaag gtgtcggcaa tacgcagggg cagccgcccg
    15421 gaagcaatga ttcggactgg gtcgatcaga cttggtcaat cggtttcaga cggcacgcaa
    15481 ctcgactggt cttggaacga aagtgccgag accgcatccg ccgccgtatc cgcgcaagaa
    15541 gtcgatccgc ttacggagta tcaggtttat aagcaattcg gttatcaggg caaggctgcc
    15601 gaatctttgg ctgcctatct ggacggcatt ccggatggtg aagcgaaacc tgagaacctt
    15661 atccgcgagc tgatcgatat caatctcgaa gtgggggatg tcgatgtttt ggcagacaat
    15721 ctgcaaaaat acggcagact gattcctttc gaacttttgg caaaatatat cgaacaggca
    15781 ttacagcgag attcaaacca tttgcgtatc cgcgtcttgg cggaagaagg tttgggatag
    15841 ggcactcagg agattgaaaa acgtgcggaa ggcggttctg cgacggcagc ttccgcatcg
    15901 cccccgccgg atgtcggcgg taaggcttat ggagccgaag aaatcaagcg catcccgatt
    15961 gtgcggggca aaaaagacgt gtccggaatc agccaagaga aaatcggtgc gattgccggc
    16021 ctggtccgcg ccgatcaagg tgcgaaaatc cttaaagaca aagtcagcta cgaaacggca
    16081 tcgaaacaat acgaccgtgc catccaaact tccgaaaaac ctgcaaacct gattatcgat
    16141 gcgttgaaac tcgattacca acacgcggac atagaccgtt ttgccggaca tttgtggaaa
    16201 ctttaccaaa cgttgggcaa ctacggcagg caggttaaag agcggatgct gggttagggg
    16261 tacagcctgg gttaccatga agttttcgat gatttggaaa agggggcgaa cgatcggcaa
    16321 atcaaagaca tcggtatgag gcacgggtat ctgccgaaaa atatacagaa attcaaagcg
    16381 caacatcggg atttggtgct tcaagattct tcgttgatca acactggttc gtctccggca
    16441 gacgatgcgg ttaaagaagt agagtcgttg ctgatgtatg gtcagattga agcggcaatg
    16501 gatgtgttgg agcaggcggt attgaaatat cccgacgaat cccagcttta tattacgttg
    16561 atcgatattt atgaacgtac tgaagattgg gataggttgg ggcagttttt aagggtattg
    16621 agggaacgtg cggacaggct tcctgaagag gtcgttatgc tgatgagccg gctgctgcag
    16681 cgtatgaatc aaaatattaa aaaaataaaa cggtacggaa aataaaaatg gaagttcaac
    16741 tgccgaaaat taaaacagta cgcgtaatgt tggcggggat gacggcgcag caggaatccg
    16801 ttttcaaaat ggcattcaaa atgcacaata ccacccgtta tgaaacagta tccccttcag
    16861 acggcagtgc cgtgcccgat ttggttttgg cggataccga tgccgagggc ggttttgaac
    16921 tttggaaaga gcttgccggg cgttataagg ctatacccgt cgccgtctgt tcggagaaag
    16981 ttcccgattc tgaagttccc tacctgccca aaccgattcg gtttgaaaca ttgtttccta
    17041 tgctccgcaa gttattgcag ggcgagaatg tttatgggaa atcgtttatt gcacccgcag
    17101 accggtcggc gaaaaacaac gggaatgtgc agcgtacggt tacgatacgc cagtttaacc
    17161 cgaataaagg attattgggg gcgttgcggt ttgcggaaaa gaacaggcag gacatcgcta
    17221 tcttgcatgg aaataagccg gtccttaatt gttttcccct cgatacaacg ggttttgctg
    17281 acagaaagtg tgtaaaaact cgaagaattg tgcaaagacg aaaatttgca ggtcagttgc
    17341 aagactgttc ccgataactc gcaatggcgc gaaaaggcta aagtaggcat tatgtcctgt
    17401 atgtggcagt tttccatttg gacggcgcag gacaggttga tttatccgat ttttcccgat
    17461 actccgttta cgttgaaatc ttggccaaac ctgacccggt aggcaaatgt gccggggtcg
    17521 atacgcttgt cggcattttt gaccaaggca tccgtcaacc ttaacgtgtt gtataaagtg
    17581 atgcctttaa acctcaatga tattctgaat tatcttgcgg caacctatac aaccgggttt
    17641 ttgtcggtag atttgaaaac ggtttcacaa caggcatact ccgatatggc ggataaaata
    17701 aatatcgggg ccgattctgc ttctgatagt gaaatgatga aaaaagcgga aaaaatcacg
    17761 acaccatccc aatcccagtc gcgcggcctt ctgcaaagac tgatgaaaaa actgttgggc
    17821 agctaagagg cggagagatg agagaaaata aaattatttt cacaggacct gtcggcgtag
    17881 ggaaaaccac tgccattgcg gctatttcgg acgaagcact cgtccagacc gatgcttccg
    17941 catccgacat gactttggat aggaaaagga atacgacagt ggcgatggac tacgggccat
    18001 cagcttggat gaggatacca aagtccattt atatagtacg cccggtcagg aacggttcaa
    18061 ctttatgtgg gaaatcttaa gccaaggcag tatgggtttg gtattgcttt tagataatgc
    18121 ccgaaccaac tcgttgaaag atttggaatt ctttttacat tcgtttcgag ggctgctgga
    18181 gaaggcatcc gtcgttgtcg gtattaccaa gatggatata cgctctcagc ccggtatcga
    18241 cgtgtatcac aaatatcttg caaaacataa tcttaatgtt ccggtttttg aaattgatgc
    18301 ccgtaaggaa gatgacgtaa aacaattggt tagcgcaatg ttattttcta ttgatccggg
    18361 actggaggtt taatatggaa tcaacacttt cactacaagc gaatttatat ccctgcctga
    18421 ctcctgccgg tgcattttat gccgtatcca gcgatgcccc cagtgccggt aaaactttgt
    18481 tgcgcagcct gttgaaagcg gatgcggacg aagtggtcag cagtgagaag ctgctcgcgg
    18541 gcggacaccg ccgacatcga taccgctttg aacctgttgt accgtttgca aaaactcgaa
    18601 ttcctctatg gcgatgaaaa cggtcattca gacggcatca atttgtcgga cgagcaattg
    18661 ccgttgctga tggaacaatt gtccggcagc ggtaaggcat tattggtcga tcggaacggt
    18721 ctgtatcttg ccaacgccaa tttccatcat gagtcggcgg aagagttggg gttgttggcg
    18781 gcagaagtcg cacagatgga aaagaaatac cggctgctga ttaggaacaa cctgtatatc
    18841 aacaataacg cttggggcgt ttgcgatcct tccggtcaga gcgaattgac atttttccca
    18901 ttgtatatcg gttcaaccaa atttattttg gttatcgccg gcattcccga tttgagcaaa
    18961 gaggcatttg ttactttggt aaggatttta taccgccgtt acagcaaccg cgtgtaaaac
    19021 ttgggacagg agggattatg cagcaattat tgatttcaat ccttgaagat ttaaataata
    19081 catctacgga tattatcgcg tctgccgtta tctcaaccga cggattgccg atggcgacaa
    19141 tgcttccttc acatttgaat tcggacaggg taggggcgat ttctgccact ttgcttgctt
    19201 tggggagtcg ctcggtgcag gaactcgcct gcggggaatt ggaacaagtg atgattaaag
    19261 gaaaatcagt ctatatcctt ttaagtcagg cgggtaaaga tgccgtgttg gtgctggtgg
    19321 caaaagaaac cggcagactt ggtttaatcc tattggatgc caaacgtgcg gcaaggcata
    19381 ttgcggaagc catataacat ataaagattg cgggcttgca gataagtgca atcgattgtc
    19441 aatttatatt gacacgttcg gtatttctgt tttattattc gcgtttactc cccgatagct
    19501 cagtcggtag agcgacggac tgttaatccg caggtccctg gttcgagccc aggtcgggga
    19561 gccaaattcc aaaaccctct aagtattttc ttagagggtt ttgttttacc ggcggtcaga
    19621 aacgcatttt tgagatgatt gttttgagat ggaataaaat ctttgcaaaa ttcctttcgt
    19681 gatggttatg aaaaataaat ttctaaagat ttaattggtt atttttataa ttttgaaatt
    19741 tatcggtttt gtaaaggtct cgggataagc aatttcggtc ttcggcgcgt cttttacgcc
    19801 aaacccataa gaattttgag ggtaatcttc atcgttttgc ctgaattgcc gacgcaaagc
    19861 gatttatcgg cggttgtgtt tgagcgtttg attgcagctt gcccaatctc aaatatttct
    19921 aaataccaat tgaaattcaa tattttataa aaacactgat ttggaacgaa cagatttata
    19981 aaaattccgt gtatccttga gtaagggagt tgattgaaaa gacttgtttg attgatgatg
    20041 aagaataagt taattaatgt cttatttggt attcgttcta ttcatatttt ttcccagttc
    20101 cttcactaaa aaatccaaaa atacgcgtaa gcggaggttg acggctttat cgctgtaata
    20161 aacagcatta aaggggtgtg ttttattgga ggtttgttcg gcgaataggg gaattaactt
    20221 tccttcagtg atgtcgttgt caaccaaaaa atctgataag caagcaatac cgcaacttga
    20281 aaggcacaac gagcgtaaga tttcaccgct gctggcggta aagtgcggtg aaattttata
    20341 gggatttccc tgcgcatcta aaaccgccca tgtatttaga gaaccgggtt ctgtgaagcc
    20401 taaacattga tggttggcaa gatcttctgc agattgtggc gtgccgtgtt ttgctaaata
    20461 ttcaggactg gcgactacgc ggaagtggct gtcaaacaga tggcgtgcac gcagcccgga
    20521 atcgtccaat tctccggccc gtaaggcaat atcgactttg cgttcaatca gattgatata
    20581 gccttcggaa gaaacgagcg aaagtcggat atgcggatag cgttcgttga attttgctgc
    20641 cagcggcgcc agcagatgca gcaccatcgg catcgcggaa tccacgcgca acacgccttg
    20701 cggtacttcg tgcactgcca gcatttcggt ttccgccgct gccatttctt gcaggattct
    20761 ctgcgcgcgg cggaaatatt gcgcgccttc ttccgtcaga ttgagttgcc gcgtggtgcg
    20821 gttgagcagg ttcacgccca acttttcctc cagccgtttg acgatgcggc ttacggcaga
    20881 atttgccatc tccaactgct ccgccgcacg gctgaagctg ccgctttcca ccacttgaac
    20941 aaatacggtc agttcttctg aattggtttt catcgtgttt ccttttcggt tggaacccca
    21001 ccctttaggg cggcaggatc agactttatt tgggaagggc gtaacccctt cccaatcagg
    21061 acggcacata gggagacgat ttatgtgtca tcccgtgtgt tgaaacatct gattattcca
    21121 tttgacgcaa aagtgttttc ttatttttgc acttttaaat cataaagtaa aacggtacaa
    21181 tacattcatc aattcacaaa cgaggtaaca aatgaatatt ttattattag acggcggcaa
    21241 agcgttcgga cattctcacg gcgggttaaa ccacacgctt cacaaaaaag cgaaagaagt
    21301 tttgaccgcg ctcggacaca atgttcaaga aaccgtgatt gatgccggct atgatgttga
    21361 ggcggaaatc gaaaaattcg tttggatgga tgctgtgatt tggcagatgc cgggctggtg
    21421 gatgcacgag ccttggacag tgaaaaaata tatggacgaa gtctttaccg gcggacacgg
    21481 caaactctac caaagcgacg gcagacaccg cgtcaatccg actgagggct acggcacagg
    21541 cggcttgttg caaggcaaaa aacatatgct ttcactgact tggaatgcgc cgattgaagc
    21601 ctttacccgc gaaggcgatt tctttgaagg caaaggcgtt gatgttttgt atatgcactt
    21661 ccacaaagcc aacgagttca tcggcttgag ccgcctgccg acattcttat gtaacgatgt
    21721 ggttaaaaat ccgcaagtgg aaaaatactt ggcagattat caggcacact tggaaaaagt
    21781 gttcggctaa ttaaaaatcc atcttcaaca cggagatgga ttttgtttgt ttcgttgatt
    21841 ttgtgtcagt ttcagatgtg ggtgcttatc cggacgggtc gtctgaaaat gtttgcccca
    21901 atgcaaaaaa atcactgcaa accttcacaa acggggctgg tagtgatttt ttcaaatcaa
    21961 acagattgaa aacctgcgcc gaattgttca gacggcatta ttttttcaat tccgccaaaa
    22021 tcccgtcaac ggttttcttc gcgtcgccga aacacatcac gctgttttcg ttgaagaaca
    22081 gcgggttttg tacgcctgcg tagccggtgt tcatcgagcg tttgaatacg acgacttctt
    22141 ttgccttcca tacttctaaa accggcatac ctgcaatcgg gctgttcgga tcggtttggg
    22201 cggcagggtt gacggtatcg ttcgcaccaa tcaccaagac cacatcggtt tcggggaagt
    22261 cgtcgttgat ttcgtccatt tccaaaacaa tgtcgtaggg gactttggct tcggcgagca
    22321 gtacattcat atgaccgggc aggcggccgg cgacggggtg gatgccgaag cgtacttcga
    22381 tgccgttttt acgcaaaagc tcggtgattt cggcaacggg gtattgtgcc tgcgccactg
    22441 ccatgccgta gcccggggtg ataatgacgc tgcttgcgcc tttcagcatt tcggcaacat
    22501 cggcagcttt gacttctcgg tattcctcta tctcttggct gccggaagac gatatgccgc
    22561 tgtcgctgcc gaaaccgccg gcaatcacgg aaacaaacga ccgattcatg gctttgcaca
    22621 taatgtagga cagaattgcg ccgcttgagc cgaccagcgc gccggtaacg atgagcaggt
    22681 cgttggagag cataaagcct gccgctgcgg ccgcccagcc ggagtaggag ttgagcatag
    22741 atacgaccac gggcatatcc gcgccgccga tggaggcaac caagtgccag ccgaatgcga
    22801 gggcgatcag ggtcataatc agaaggatga agccgctgcc gtcaatgccg acaaatacga
    22861 gcagcaacac aaacgatacg gcgagcgcca gtgcgttgag cttgtgtttg gcgggcagtt
    22921 gcagcgggct gctgctgatt ttgccgttga gtttgccgaa tgcggccagt gagccggtaa
    22981 aggttaccgc gccgatgaaa atgcccaaat agacttcgac cagatggatg gtgtgcatat
    23041 cgtgcgaaac gttgcccggc tcgatgtagc tgttgaagcc gaccaaaacg gctgccaagc
    23101 cgacgaagct gtgcagaagt gcaatcagtt cgggcatttc ggtcatttct acttttttgg
    23161 ctttgtggat gccgattgcc gcgccgatga gcatggcgat gataatccag cccagtccgt
    23221 gggtattgtc ggaaaagacc gttacgaaga gggcgaccgc cataccggcg ataccggaat
    23281 agcagccccg tttggcggtt tcctgtttgg acagccccgc cagtgagaag atgaataaaa
    23341 ttgcggcaac gatatacgcc gctgttacga gtcctgaaga catggaaatt ctccgatttt
    23401 cgatgatttg ttttcaatgc cgtctgaaaa attgacgttc gtgttttcag acggcatctg
    23461 tttcaagcag ccgcgacaaa cagtccgacg gtgcaggcaa atccgcccgc ccacatcagc
    23521 gagcgcaatg ctgctttgtc tgcgatgtag caccagataa aggcgaggcg gaacaggatg
    23581 aacaatccgg caagcgtgtt gacggttgct tgtccggcat tgccggttgc gtgtgccgtc
    23641 aaaacggcgg cggcaaacgg tgcaaaggct tcaaaaccgt tttgctgcgc ggcgtgggca
    23701 cgggcggctg cgccttgcgt atgtgccaga aaaccgcgag gattgtggtt gtctttaaac
    23761 cggaatccgc ctgctttttt ggcatacgcc gcacaaaaaa gcggcaatag gcaggcaatc
    23821 agaatgcacc aataggcgaa agtcatggct taccctttct taaacatatt cagcatacgc
    23881 cgtgtaaccg caaagccgcc gaagatattg atgccggcaa tcaggatggc aacaaacgac
    23941 agcagcgaaa cgaagccgtt gccctgaccg atttgcagca gcgcgccgac gacgatgatg
    24001 ccggagatgg cgttggttac cgacatcagc ggtgtgtgca gcgagtggct gacgttccaa
    24061 acgacatggt agccgatgac gcaggcgagg acgaagacga taaagtggtt caagaatgct
    24121 gcgggtgcga ccgcgccgac ccacagcacc aatacggcgg cgatggcggc gggcgcgagt
    24181 tttttccaca ggggaacagg tttcggctcg ggcttggcgg caggcgcggc tttttcagac
    24241 ggcgtttgct gcggccgggc ggaaacctga atcggcggag gcgggaaggt gatttcgccg
    24301 tcgcgggtaa cggtcatatt gcggataatc acgtcttcga agtccagcgt gatttcgccg
    24361 tctttgttcg ggcttaacag cttggtcagg ttcaccaagt tggtggcgta aagctgggaa
    24421 gactgtccgg caaggcggtt tgccatgtcg gtgtagccga tgattttcac gccgttgccg
    24481 gttacggaca attcgcccgg tcgggtgagt tcgcagttgc cgcccgtcgc cgccaaatcg
    24541 acgatgacgg atccggattt catgctttcc accatttctt tggtaatcag cttgggagcg
    24601 ggtttgcccg gaatggcggc ggtggtgatg atgatgtcca cttctttcgc ctgttcggca
    24661 aagagcttca tttcggcggc gataaattcg tcgctcatca ctttggcgca gccgtctccg
    24721 ctgccgcccg attcttgcag gaagtcgagt ttcaggaact taccgcccat cgattcgatt
    24781 tgttccgcca cttccaagcg ggtatcgaac gcgcgcacca ctgcgccgag cgaatttgcc
    24841 gtaccgattg ccgccaaacc cgccacacct gcaccaatca ccaaaacctg cgcaggcggc
    24901 actttgccgg cggcagtgat ttgaccggtg aagaaacggc cgaaggcgtt ggcggcttca
    24961 atcacggcgc ggtagccgct gatgtttgcc attgaagaca aagcgtccaa ggcctgagcg
    25021 cgggaaatgc ggggaaccat gtccatcgcc agcgcgttga ctttcttggc gcgcaaggcc
    25081 tcgaccaaag cctcgttttg gcgcggccac aggaagctga cgatggtttg accttctttg
    25141 agcagcggca gctcgccttc ggacggcgcg ttgaccttat aaattaaagg gcaggcccaa
    25201 accgccgctt tgtcggcaac ggttgcgcct gctgtttggt aagcggcatc gtccaaactt
    25261 gccgccaaac ctgcaccgct ttcgacaacg gtttcaaagc ctagtttgcc cagcagggca
    25321 acggtggcgg gcgtgcaggc gacgcgggtt tcgccggata atgactcgcg tgggataccg
    25381 attttcatct ctgaatcctt tttcgggttg ttcatatgta tcgtgggtta aatttaaatc
    25441 ggtgcagggc ggagcaacgc cgtaccggtt taaaaccgat tcacttcaaa tgttaatata
    25501 ttttagataa tccccttata acgaattttc atcaggctgg caatagttgc gcattttccc
    25561 gtgttgtccg acacatactg ccgtttgcgt ttgaagtcgt tttccgattg caggcacgcc
    25621 cgccgtaaca ttctttgccg tttcactata taatccgcat tttttgagcc gccgttatgc
    25681 cgcaagccct cgtcctccaa tttccctctg ccgaagccct gccttccgac ttcccctcac
    25741 gcctgcccga acctgattat gctgatgaaa agcgtatgcg ttttattgtt gaagaaggat
    25801 tttctttaag cgaaaaagac gcggcgttac tggacagccg tcaaatcgac cacgccgttt
    25861 tgcccaatat ggcgtttggc gaactcgggc tgattgtcag cgatatggat tcgacgctga
    25921 ttaccatcga atgtatcgat gaaattgcgg caggtgtggg tttgaaaggc aaagtagcgg
    25981 aaattaccga gcgcgccatg cgcggagagt tggatttcga acagtcttta cgcagccgcg
    26041 ttgcgttgtt ggcgggattg gacgaacaga ttttggcgga catttatgaa aacgttttga
    26101 agctctcgcc cggtgcggaa tttttgttgg acgaatgcaa aaggcacaat gtgaaattcc
    26161 tgctggtgtc cggcgggttc acgtttttta ccgaaaggct gcaacaacgg ctcggcttcg
    26221 aataccaaca cgccaatatt ttggaaattg aaaacggcag gttgaccggc cgtctgaaag
    26281 gcagaatcat cgacgcgcag gcaaaggcgg atttattgcg cgaataccgc agccgcctcg
    26341 gattgcagcc gcatcaggtg ttggcgatgg gcgacggtgc gaacgatatt ccgatactca
    26401 aagaagcggg cataggcgtg gtttaccgcg ccaaaccgaa agcgcgggcc gccgccgatg
    26461 cctgtatcaa cttcggcggt ttggagcgtg tacgcggcct gttcggatag gcggatagga
    26521 aacggatgcc gtctgaaagg ttttcagacg gcatttgaac ggcaggaacg acagtgggac
    26581 gcagaaaact ttggtttgcc ctggcggcag caggcgttat cggcggtttg gtcggcattg
    26641 tgctgacgga actgatgcac ttcatacggc atacggcata cggttatgac gcggacggtg
    26701 cgtacatctc gttccgcgaa ggtgtggcac aggctttcgg tatgcggcgc gttgccatgc
    26761 tgacgctgtg cggcgcgatc gcaggcggcg gctggtggct gctcaaacgt ttcggcaagc
    26821 cgcaaatcga aatcaaagcc gccttgaagc agccgttgca ggggctgccg tttctgacga
    26881 cggttttcca tgttctgctg caaatcataa cggtcggact cggttcgccg ctcggacgcg
    26941 aagtcgcccc gcgtgaaatg actgccgcgt ttgcttttgc cggcggcagg cggttgggtt
    27001 tggacgaagg cgaaatgcgc ctcttgattg cctgtgcgtc cggagcgggt ttggcggcgg
    27061 tgtataacgt gccgctcgcc tccacgctgt ttatcctcga agcgatgttg ggcgtgtgga
    27121 cgcagcaggc ggttgccgcc gcattgctga cttccgtcat cgccaccgcc gtcgcgcgca
    27181 tcggcttagg cgacgtgcag cagtatcatc ccgccaacct tgccgtaaat acttcattgc
    27241 tttggttttc cgccgtcatc ggcccgatac ttggcgcaac cgccgccttt ttccggcgca
    27301 ccgcccaaaa attccccttc atcaagcgcg acaatatcaa aatcattccc ttggccgtct
    27361 gtatgtttgc actcatcggt gtgattgccg tttggtttcc cgaaattttg ggcaacggca
    27421 aagcaggcaa ccaactgact tttggcggtt tgaccggctg gcaatacagc cttgagttga
    27481 ccgctgtcaa atgggcggtc gttttgatgg cgctggcggc aggcgcatac ggcggactga
    27541 ttacgccgtc tatgatgctc ggcagcacca tttcttttgc agccgccgcc gcgtggaaca
    27601 gcgtcttccc cgaaatgccg tctgaaagcg cggcagtcgt cggagcggcg gtgtttctcg
    27661 gcgtatcgct caaaatgccg ctgaccgcca ttgtgtttgt attggaactg acctacgcgc
    27721 cgctgtccct gctgctgcct ttgtgcatcg gcatggcggg ggcattggga acatcgggaa
    27781 aaatgggctt caaatgaatg ccgacaaatg ccgtctgaaa ggtttccaga cggcatttct
    27841 ttgcaaattg tcaacaatat ctgcaaaaac ctactgccaa aaaactgaaa atagcggata
    27901 ataccgcccc gaaaaagatc ccctttcacc atccaaccgt cagaaaagga agccccaatg
    27961 tccgtcatca aacgcgccct gatcagcctg tccgacaagg caggcgcggt tgaatttgcc
    28021 caaaacctgc acaagctcgg tgtcgaaatc ctctccaccg gcggcacggc gaaactgctt
    28081 gccggtgcgg gcgttcccgt gattgaagtc gccgactaca ccggttttcc cgaaatgctc
    28141 gacggccgcg tgaaaaccct gcatccgaaa atccacggcg gtattttggg ccggcgcgat
    28201 ttggacgaac acgtcgccaa gatggaagag cacggcatcg gcaacatcga ccttgtgtgt
    28261 gtcaatcttt accccttcgc cgccaccatc gccaagcccg gctgcacact ggaagacgca
    28321 attgaaaaca tcgacatcgg cggcccggcc atggtgcgtt ccgccgcgaa aaactggaaa
    28381 cacgtcgcca tcgttaccga caccgccgac ttccccgcca ttgccgccga actcgaagcc
    28441 ggcaacggcg cgttgagcga taaaacccgc ttcaacctct cacgcaaagc attcagccac
    28501 accgcccaat acgacggcat gatttccaac tacctgacct ccctttcaga cgacgttttg
    28561 agcggccagc cccaaatcgg cgaattccca agccagttca accaaagctg gattaaagtg
    28621 caagacatgc gctacggcga aaacccgcat cagcgcgccg cgttttaccg cgatattgac
    28681 cccgccgcag gcagcctttc cgcctacaat caactgcaag gcaaagaatt gtcttacaac
    28741 aacatcgccg atgccgatgc cgcttgggaa gccgtcaaat cctttgacgc gcccgcctgc
    28801 gtgatcgtga aacacgccaa tccgtgcggc gtagcggttg cagccgatac cttgaccgcc
    28861 tacaaactcg cctacgccac cgacaccacc agcgcgttcg gcggcatcat cgccttcaac
    28921 cgcgaagtcg acggcgaaac cgtcaaacag attaccgaca accaatttat ggaagtcctc
    28981 atggcgccga agtttaccgc cgaagccctc gaaatcgccg ccgccaagaa aaacgtgcgc
    29041 gtattggaag tgccgctcaa agcaggtgcc aaccgcttcg aactcaaacg cgtcggcggc
    29101 ggactgttgg tacaaacccc cgacatcaac cgcatcaacc gcgccgattt gaaagtcgtc
    29161 tccaaacgcc aaccgaccga gcaggaatgg aacgatttgc tgtttgtctg gaacgtcgcc
    29221 aaatacgtca aatccaacgc catcgtgttc ggcaaaggcg gtcaaaccta cggcatcggc
    29281 gcaggccaaa tgagccgcgt ggacagtacc cgcatcgccg cccgcaaagc gcaagatgcc
    29341 ggtctcgacc tcaacggcgc atgtgccgct tccgatgcct tcttcccatt ccgcgacggc
    29401 gtggatgtca ttgccgagca gggcatcaaa gccatcatcc atccggcagg ctcgatgcgc
    29461 gatcaggaag ttttcgacgc ggcggacgaa cacggcatcg ccatggcggt aaccggcatc
    29521 cgccacttcc gccattaagg cagatgaata aggcaatgcc gtctgaaggg ttttcagacg
    29581 gcattttgcg ccatttaccg taatcctgaa atcccgtcat tcccacgcag gcaggaatct
    29641 agatgagaat ccatcctgtc agaaataccc gaatcgtcag aaatacccgt gtcatcattc
    29701 ccgatacacc gtaatcctga aacccgtcat tcccgcgcag acagaaatcc ggatttgtcc
    29761 gcgcggaaac ttatgcgccg ccattcccgc gaaggcggga atccggaccg ttcggtttcg
    29821 gttatttccg ataaattcct gctgcttttc attcctagat tcccactttc gcgggaatga
    29881 cgaaggagtg ggaatccggt ttttgagttc cggccatttc cgacaaattg ctttggcatt
    29941 ggatatttcc tattttaatc cactatagtg gattaacttt aaaccagtac g
//
LOCUS       DBJGGI010000021        27319 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.021, whole genome shotgun sequence.
ACCESSION   DBJGGI010000021 DBJGGI010000000
VERSION     DBJGGI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27319
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.021"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(<1..258)
                     /locus_tag="ACSIGJ_000910"
     CDS             complement(<1..258)
                     /locus_tag="ACSIGJ_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329809.1"
                     /translation="MNTKTELQKLLEEDISTLKETLIRVDALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYS"
     gene            complement(320..2944)
                     /gene="alaS"
                     /locus_tag="ACSIGJ_000911"
     CDS             complement(320..2944)
                     /gene="alaS"
                     /locus_tag="ACSIGJ_000911"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HHQ1329810.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGKEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAPFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVQEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLGGEI
                     IFKLYDTYGFPYDLTADICRERNIEPDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGGQVVELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFAGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGGHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTDAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALRETVTDLTGKSDNA
                     VILLAAVNEGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAD
                     KLPEMLASAEGWLCQKLS"
     gene            3148..4278
                     /locus_tag="ACSIGJ_000912"
     CDS             3148..4278
                     /locus_tag="ACSIGJ_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HHQ1329811.1"
                     /translation="MVKHLPLAVLTALLLAACGGSDKPPAEKPAPAENQNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSMIPNYKHLNPEMMRLMDGVDPDHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFNPEYTFKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARDLMEDE
                     FKNDNTIFPSGEDLKNSFIMVPIRPAALKFMVRQWQDVKAGK"
     gene            complement(4319..5221)
                     /locus_tag="ACSIGJ_000913"
     CDS             complement(4319..5221)
                     /locus_tag="ACSIGJ_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HHQ1329812.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTVMNVLIKEASAKFALGSGELVF
                     WRMLFSTVTLGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLTTGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQEPAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSATGVAMSSVWATLTGWHTLSFPSAVYLSGI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPIAFKQRLQALFRQR"
     gene            5369..5857
                     /locus_tag="ACSIGJ_000914"
     CDS             5369..5857
                     /locus_tag="ACSIGJ_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HHQ1329813.1"
                     /translation="MKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLAPSHWADVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAVDSQRGEINTEQSKLYIENALRGWQQRWKNMDAKPDNPAFTNFLMEVMKMQP
                     LK"
     gene            complement(5919..6824)
                     /gene="mtrA"
                     /locus_tag="ACSIGJ_000915"
     CDS             complement(5919..6824)
                     /gene="mtrA"
                     /locus_tag="ACSIGJ_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HHQ1329814.1"
                     /translation="MDILDKLVDLAQLTGSADVQCLLGGQWSVRHETLQCEGLVHIVT
                     AGSGYLCIDGETSPRPVGTGDIVFFPRGLGHVLSHDGKYGESLQPDIRQNGTFMVKQC
                     GNGLDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESEKPLTGTVS
                     VVNALPSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNIDKM
                     VAAANMSRAQLMRRFKSQVGLSPHAFVNHIRLQKGALLLKKTPDSVLEVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            6953..7288
                     /locus_tag="ACSIGJ_000916"
     CDS             6953..7288
                     /locus_tag="ACSIGJ_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HHQ1329815.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            7419..7889
                     /locus_tag="ACSIGJ_000917"
     CDS             7419..7889
                     /locus_tag="ACSIGJ_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329816.1"
                     /translation="MKYHRLALFAAISCLLLSAVFIAPYLTAFHEQEKIFEYADLTVT
                     APNRSGRAIKLEADGRQYRLSCYGFDSLCTGGNIGRAIRARQVKIVLSETVGKGFLNG
                     VLLEYRNSGSVYSNKDFSRTEDRLVEVLAQPAVFSLKPGILLLLPAIFLRLKKM"
     gene            7995..8558
                     /gene="pgsA"
                     /locus_tag="ACSIGJ_000918"
     CDS             7995..8558
                     /gene="pgsA"
                     /locus_tag="ACSIGJ_000918"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HHQ1329817.1"
                     /translation="MPWNIPIFLTWLRVLLIPVLIVLFYLPFSWFSEEAVNVAAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRSSVAVATVGKFKTAAQMLAIFFLLLNFPDFYGFNLVVIG
                     NILMFIASLLTVWSMLYYLKMAWKEIA"
     gene            8639..8714
                     /locus_tag="ACSIGJ_000919"
     tRNA            8639..8714
                     /locus_tag="ACSIGJ_000919"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8672..8674,aa:Gly,seq:gcc)
     gene            8740..8815
                     /locus_tag="ACSIGJ_000920"
     tRNA            8740..8815
                     /locus_tag="ACSIGJ_000920"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8773..8775,aa:Gly,seq:gcc)
     gene            8841..8916
                     /locus_tag="ACSIGJ_000921"
     tRNA            8841..8916
                     /locus_tag="ACSIGJ_000921"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8874..8876,aa:Gly,seq:gcc)
     gene            8942..9015
                     /locus_tag="ACSIGJ_000922"
     tRNA            8942..9015
                     /locus_tag="ACSIGJ_000922"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8974..8976,aa:Cys,seq:gca)
     gene            9116..9205
                     /locus_tag="ACSIGJ_000923"
     tRNA            9116..9205
                     /locus_tag="ACSIGJ_000923"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9150..9152,aa:Leu,seq:taa)
     gene            9309..10361
                     /gene="sohB"
                     /locus_tag="ACSIGJ_000924"
     CDS             9309..10361
                     /gene="sohB"
                     /locus_tag="ACSIGJ_000924"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HHQ1329818.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFETFFLSEEETKHQEKKEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIIGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPGLDIEKI
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKRSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(10412..11278)
                     /locus_tag="ACSIGJ_000925"
     CDS             complement(10412..11278)
                     /locus_tag="ACSIGJ_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HHQ1329819.1"
                     /translation="MWKMLKHIAKTHRKRLIGTFSPVGLENLLMLGYPVFGGWAINAV
                     IAGRVWQALLYALVVFLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLNNSLERDNHFIRKGDERQLYRHYGLVSRLRVLISNREAFGYLCVG
                     AAMGILFGFAFVMMTLKGYGSAGHIYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQR
                     IEWSERNIKAGT"
     gene            complement(11289..11720)
                     /locus_tag="ACSIGJ_000926"
     CDS             complement(11289..11720)
                     /locus_tag="ACSIGJ_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HHQ1329820.1"
                     /translation="MNRLDQLGIRINLICNVFDKWIGQQDLNYNLFTVLYTLATEGSR
                     TQKHIGEEWSLPKQTVSGVCKTLAGQGLIEWQEGEQDRRERLLSLTEKGKVHAAPLTE
                     NAQEFSDKVFSTFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            complement(11955..12824)
                     /locus_tag="ACSIGJ_000927"
     CDS             complement(11955..12824)
                     /locus_tag="ACSIGJ_000927"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HHQ1329821.1"
                     /translation="MSAETYTQIGWVGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGSTAELVRACPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(12900..13811)
                     /gene="hisB"
                     /locus_tag="ACSIGJ_000928"
     CDS             complement(12900..13811)
                     /gene="hisB"
                     /locus_tag="ACSIGJ_000928"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HHQ1329822.1"
                     /translation="MTKTQLHLNNFLTLAQEAGSLPKLAKLCGYRTPVALYKLKQRLE
                     KQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETRIAETA
                     SAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDIDIS
                     CKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLSGR
                     PGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFKAF
                     GRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(13828..14907)
                     /gene="hisC"
                     /locus_tag="ACSIGJ_000929"
     CDS             complement(13828..14907)
                     /gene="hisC"
                     /locus_tag="ACSIGJ_000929"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HHQ1329823.1"
                     /translation="MKSVRSFIRNDILAMSAYKITDVPPGFAKLDAMESPAHPFEGHE
                     ALMREWRAQLASAPIHLYPNPSGCGLQEALRSAFDIPDCAAVALGNGSDELIQFITML
                     TAKPGAAMLAAEPGFIMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAAIEASDGIVVVDEAYGAFNGDSFLPQAGRIPNLIVLRTL
                     SKIGFAGLRIGYATGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNAFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITIGSSAQNDAVLDVIRRLYR"
     gene            complement(14953..16242)
                     /gene="hisD"
                     /locus_tag="ACSIGJ_000930"
     CDS             complement(14953..16242)
                     /gene="hisD"
                     /locus_tag="ACSIGJ_000930"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HHQ1329824.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAIAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILVKDLNEACEISNYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(16239..17126)
                     /locus_tag="ACSIGJ_000931"
     CDS             complement(16239..17126)
                     /locus_tag="ACSIGJ_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEM-3-like GIY-YIG domain-containing protein"
                     /protein_id="HHQ1329825.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLSEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMTEETKNGRKQTRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGGKAYIRPKTE
                     QETLQTIPSPKITKENPKS"
     gene            complement(17218..17883)
                     /gene="hisG"
                     /locus_tag="ACSIGJ_000932"
     CDS             complement(17218..17883)
                     /gene="hisG"
                     /locus_tag="ACSIGJ_000932"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HHQ1329826.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIAPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSYLVVNKAALKTKYALLEPIIQSFGGAVKAKW
                     AFI"
     gene            complement(17962..18615)
                     /locus_tag="ACSIGJ_000933"
     CDS             complement(17962..18615)
                     /locus_tag="ACSIGJ_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HHQ1329827.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASAPAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKINQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            18891..18975
                     /locus_tag="ACSIGJ_000934"
     tRNA            18891..18975
                     /locus_tag="ACSIGJ_000934"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18925..18927,aa:Leu,seq:caa)
     gene            complement(19032..19145)
                     /locus_tag="ACSIGJ_000935"
                     /pseudo
     CDS             complement(19032..19145)
                     /locus_tag="ACSIGJ_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            19669..21396
                     /gene="ilvB"
                     /locus_tag="ACSIGJ_000936"
     CDS             19669..21396
                     /gene="ilvB"
                     /locus_tag="ACSIGJ_000936"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HHQ1329828.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELVETIKKAFQIAASGRPGPVVLDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNILSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRTDGKA"
     gene            21407..21898
                     /gene="ilvN"
                     /locus_tag="ACSIGJ_000937"
     CDS             21407..21898
                     /gene="ilvN"
                     /locus_tag="ACSIGJ_000937"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HHQ1329829.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            21965..22258
                     /locus_tag="ACSIGJ_000938"
     CDS             21965..22258
                     /locus_tag="ACSIGJ_000938"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HHQ1329830.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYNLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAV"
     gene            22338..23351
                     /gene="ilvC"
                     /locus_tag="ACSIGJ_000939"
     CDS             22338..23351
                     /gene="ilvC"
                     /locus_tag="ACSIGJ_000939"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HHQ1329831.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRHGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVAELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGARLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(23470..24465)
                     /locus_tag="ACSIGJ_000940"
     CDS             complement(23470..24465)
                     /locus_tag="ACSIGJ_000940"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HHQ1329832.1"
                     /translation="MNLKNRHFLKLLDFTPEEITTYLDLAAELKDAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGADATYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFAQETVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKGVGFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPAGIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(24607..27192)
                     /gene="acnB"
                     /locus_tag="ACSIGJ_000941"
     CDS             complement(24607..27192)
                     /gene="acnB"
                     /locus_tag="ACSIGJ_000941"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HHQ1329833.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKNPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLVSPKYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVNILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKVGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVIPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRALERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKSDIPVRLWVAPP
                     TKMDAKELSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
BASE COUNT         6217 a         6684 c         7512 g         6906 t
ORIGIN      
        1 actataaaaa ttaactttat tctctaaggt tgacaatttt tcaaatacca aacttgtatc
       61 aaggatgggc aattctatag ttaaacctat ttcttttgcg agaatattta attgtttgtc
      121 aattaaccat cgacggacaa taggagttgc aatgctacgt acatatcttg gaggaagagc
      181 atctacacga attagtgttt cttttaaagt tgatatatcc tcttctaaaa gtttttgtaa
      241 ctctgtttta gtattcattc taacaatcca aaatataagc catttgaaca agactcaaat
      301 ggcttattaa gatttaaatt taacttaatt tctgacaaag ccaaccttcc gcgcttgcca
      361 gcatttcggg caatttgtcg gcatccgtac cgccggcttg cgccaagtcg ggacggccgc
      421 cgcctttgcc gccgacttgt tcggctgcaa atttaaccag atctcctgct ttgacttttc
      481 cggtcagggc tttggatacg ccggcgcaca gggagacttt accctcgttt actgccgcta
      541 aaagaatcac ggcgttgtcg gattttccgg tcaggtcggt tacggtttcg cgcagggcgg
      601 ctgcgtcggc ttcgatttgt gcggcaacga gtttggccgc gcccaggtct tttgcgtcgt
      661 ccaagagttt ggcgcctgcg tggacggcga gttcggcttt ggcgcgtgcc aattcttttt
      721 ccaatgcttt ggcgtgtgcc gcgcctgctt ggattttcgc cagtacgtct ttttcggttt
      781 gggctttggt ttcggcaatg atgtctttta ccaaacgctc ttgctcttgc gcccatttga
      841 gtgcgttcag gccggtgatg gcttcgatac ggcgtacgcc tgcggcaata ccgccttcgc
      901 tgatgatttt gaagaggccg atgtcgccgg tgcgtgaaac gtgtgtgccg ccgcacaatt
      961 cggtagagaa accgcccatt tgcagtacgc gtacttcgtc gccgtatttt tcgccgaaga
     1021 gcatcatggc gtcggttttt tgcgcgtctt ccatgctcat aatggctgcg ttgacggcaa
     1081 cgttggctaa aacggcttcg ttgacgcggc gttcgacttc agcgatttct tcggcggtta
     1141 ccgcttgggg atgggaaatg tcgaaacggg tggattcggc ggtaaccaaa gagccttttt
     1201 gttcgacgtg tccgcccaat acgtcgcgca gggctttgtg catcaggtgg gtcgcgctgt
     1261 ggttgcgcat attggcattg cggatttcgt catccacttt ggcggtaacg ctgtcgccga
     1321 ctttcagacg gcctgaagtt tgtacgccga attgtccgaa tacggccgct ttgatttttt
     1381 gggtatcgcg tacttcaaag cggttttcgc ctgcgaagat atagccgacg tcgccgactt
     1441 ggccgccgga ttctgcatag aacggggtaa agtcgataac gacggcaccg ctgtcgcctt
     1501 cgttcaattc gacgacttgc ccgccgtctt tgtagagggc gaggactttg gattcggttt
     1561 ggcgttcgct ataaccttta aactcggtgt cttgaccgtc gtaaggcagt tgggcgttgg
     1621 ctttgaagct ttgggcggcg cgtgcgcgtg cgcgttgggc ttccatttcg cgctcgaagc
     1681 ctgcttcgtc cggttcgata ttgcgttcgc ggcagatgtc ggcagtcagg tcgtatggga
     1741 agccgtaggt atcgtagagt ttgaagataa tttcgccgcc gagtgttttg ccgcctttgg
     1801 ccagcgcgtt ttccaacaaa gccataccgg tttccagagt ttgggcaaaa cggctttctt
     1861 cgtttttcaa agcttcttcg atttgcgcct gtttttcttt caattcagga tacgcgccgc
     1921 ccatctcttg aaccaaatcg gcaacgagtt tgtggaagaa cggtttgctt tgacccagtt
     1981 tgtaaccgtg gcgcacggcg cggcggatga tgcggcgcaa tacgtagccg cgtccttcgt
     2041 tggaaggcaa tacgccgtct gcaatcagga atgagcagga gcggatgtgg tcggcgataa
     2101 ctttcaggct gggttcttcc atactgaacg gcgcgccggt ttcgcgggca acggctttga
     2161 gcaggtcttg gaacaggtcg atttcgtagt tgctgtggac gtgctgcatc acggcggcca
     2221 tacgttccaa gcccataccg gtatcgacgg acggcttggg cagtggattc atattgcctt
     2281 gttcgtcgcg gttgaactgc atgaatacgc agttccaaat ttcaatccag cggtcgccgt
     2341 cttcttcggg gctgccggga atgccgcccc agatttcttt gccgtggtcg tagaaaattt
     2401 cggagcaggg gccgcaaggg ccggtgtcgc ccatttgcca gaagttgtcg gacgcgtatt
     2461 tcgcgccttt gttgtcgccg atgcggacga tgcgttcaga cggcataccg atttcgttca
     2521 accagatgtt gtaggcttcg tcgtcttctg cgtaaacggt cgccaagagt ttgtctttgg
     2581 ggatgttgag ccattcgggg gaagtgagaa actcccaagc gaagtggatc gcgtcgcgtt
     2641 tgaagtaatc gccgaaggag aagttgccca tcatttcaaa gaaggtgtgg tggcgggcgg
     2701 tgtagccgac gttttccaag tcgttgtgct tgccgcctgc gcgtacacat ttttgcgcgg
     2761 tggtggcgcg gctgtacggg cgtttgtcga aacctaaaaa tacgtcttta aactggttca
     2821 tgcctgcgtt ggtaaacagc agggtcgggt cgtcgtgcgg cacgaggctg gaagagcgga
     2881 cgacggtgtg gcctttggtt tcaaaaaatt ttaggaattt ttggcgcagt tcggaggttt
     2941 tcataatttt ttcaatgtct ctcaaatgtc ttgtcatggt aaaagcaggg aaaacgaacg
     3001 gcggtatatt accgcaaatc cctgtttcta gctatggaag cggcggcttt cagacggcat
     3061 tgcgggattt tgaatgccgt ctgaagccct gttaccaata tcggctataa tggccgcttt
     3121 ctccaacccg atatgcaagg aatgataatg gtcaaacatc tgccactcgc cgtcctgact
     3181 gctttgctgc ttgcagcgtg cggcggttcg gacaaaccgc ctgccgaaaa accggcaccg
     3241 gcggaaaacc aaaacgtatt gaaaatttat aactggtcgg aatacgtcga tccggaaacc
     3301 gttgccgatt ttgaaaagaa aaacggcatc aaggttactt atgatgtgta cgacagtgat
     3361 gaaacgctgg aaagcaaggt gctgaccgga aaatccggtt acgacattgt cgcgccgtcc
     3421 aatgcgtttg tgggcaggca gattaaggca ggtgcgtatc agaaaatcga taagtcgatg
     3481 attcccaatt ataaacatct caaccctgaa atgatgaggc tgatggacgg ggtcgatccc
     3541 gaccacgaat acgccgtgcc gttttattgg gggacaaata ccttcgccat caataccgaa
     3601 cgcgtgaaaa aggctttggg tacggacaag ctgccggaca accagtggga tttggtgttc
     3661 aaccccgaat acacgttcaa actcaaacaa tgcggcatca gctatttgga cagcgcggcg
     3721 gaaatttatc ccatggtgtt gaactatttg ggtaaaaacc cgaacagcag caatacggaa
     3781 gacatcaggg aggcaaccgc cctgctcaag aaaaaccgcc ccaatatcaa acgctttact
     3841 tcgtccggct ttatcgatga tttggcgcgc ggcgatacct gcgtaacaat cggtttcggc
     3901 ggagatttga acatcgccaa acgccgtgcc gaagaagcgg gcggcaagga aaaaatccgc
     3961 gtgatgatgc cgaaagaggg cgtggggatt tgggtggatt ctttcgtgat tccgaaagat
     4021 gcgaaaaacg tcgccaacgc gcacaaatac atcaacgact tcctcgatcc ggaagtgtcg
     4081 gcgaaaaacg gcaatttcgt tacctacgcg ccttcgagca agccggcgcg cgatttgatg
     4141 gaggacgaat ttaaaaacga caatacgatt ttcccgagcg gggaagattt gaaaaacagc
     4201 tttatcatgg tgcctatccg gccggcggca ttgaagttta tggtgcgcca gtggcaggat
     4261 gtgaaggcgg ggaaataaag cccgatatgc cgtctgaagg atgttcggac ggcatttttt
     4321 atctttggcg gaagagggct tgcagccgct gtttgaaggc aatggggcgg atgctgctca
     4381 aaatgccgct gaggataatg atgcacatac cgagtatttc ctgccagaaa agctcttcgc
     4441 ccagaaaaaa tgcggcagac agggcggaaa agacgacggt catataggaa agcgaggcaa
     4501 ccgtgaattt gtcgccgact ttgtaggcgc gcgtcatcga cagttgggca atcagcgcgg
     4561 acacgccgat gcccgacaga taaaccgccg atggaaagga cagggtgtgc cagccggtca
     4621 gcgtcgccca aaccgacgac atcgccacgc cggttgcgga aaggtaaaac acgacgcgcc
     4681 agccgggttc gcccgccaaa gacagttcgc gcactttcaa atacgcccag ccggacatcg
     4741 cgccgcccgc cagcccggcg agtgccgccg gttcctgacc gctgcggaac gagggattaa
     4801 gcagcaatac cacgccggca aaaccaagga gcagcaccgc ctgcgtgtaa acggaaatcc
     4861 gttctttcaa aatcaggaag gaaaataccg ccaaaaaaat cgacgaggtg taactcaggg
     4921 taacgccggt tgtcaaaggc agatgcgtta ccgcgtaaaa cagcagcagc atcgcccccg
     4981 tcccgaccat actgcggttt aagtggtttt tccaatgggg cgtgcggaag gtgtcgcgcc
     5041 gcaatacggc ggcagcaccg agcgtaacgg ttgaaaacag catgcgccaa aagaccaatt
     5101 cgccgctgcc gagggcaaat tttgccgatg cctctttaat caatacgttc ataacggtga
     5161 agcaggccgc cgccaccagc atccagcccg atcctaaaat gtcttttttt gcggtatcca
     5221 taaacggtcg ttgcgataag gacggtcgga ttgtaaacct tgcggcaagg cttgtggaat
     5281 gtgtttttgc ctgcttctga tgccgaaatt ttatttttct tgccgaacaa tttgttttct
     5341 caaggcaaac ttgattataa tggcgggtat gaaaaaatac cttatccctc tttccattgc
     5401 ggcagtcctt tccggctgcc agtctattta tgtgcccaca ttgacggaaa tccccgtgaa
     5461 tcctatcaat accgtcaaaa cggaagcacc tgcaaaaggt tttcgcctcg ccccttcgca
     5521 ttgggcggat gttgccaaaa tcagcgatga agcgacgcgc ttgggctatc aggtgggtat
     5581 cggtaaaatg accaaggttc aggcggcgca atatctgaac aacttcagaa aacgcctggt
     5641 cggacgcaat gccgtcgatg acagtatgta tgaaatctac ctgcgttcgg cggtagacag
     5701 ccagcgcggc gaaatcaata cggaacagtc caagctgtat atcgagaatg ccttgcgcgg
     5761 ctggcagcag cgttggaaaa atatggatgc caaacccgat aatcccgcat ttaccaactt
     5821 tttgatggaa gtgatgaaga tgcagccctt gaaatgacgc ggtacgcaaa tgccgtctga
     5881 aagctttttc agacggcatt tgcgtttgaa gccccgattt atttttgccc gccttctttc
     5941 cggtattgcc ccggcgaaac gtgatattgc cgtttgaacg ccttgccgaa atgcgtttcc
     6001 gactgaaagc ccaccgacag cgcgacctcc aaaaccgaat ccggggtttt cttcagcagc
     6061 aatgcgcctt tttgcaggcg gatatggttc acaaaggcgt gcgggctgag tccgacttgg
     6121 cttttgaagc ggcgcatcag ttgcgcgcgc gacatattgg cggcggcaac cattttgtca
     6181 atattccatt cgtcttccgg tttgtctatc accttttgga tcaaatgtcc caaacgtttg
     6241 tcctgccaac ctttcaatac gcccgagagt tcgacatcct tatcctgttc gagataggcg
     6301 cgcaggataa gcaccagcag gacggacggt aatgcgttga ccacggaaac cgtccccgtc
     6361 aaaggttttt cgctttccag ttgcagcatt gaaaccacat actgcaaact tggatgggca
     6421 atgttcagaa aaacggtttc cggcagcccg ttcatcaaat cggcgtgggt gtcgtagcgg
     6481 aaacgggcgc aaaacaggct catatccagc ccgttgccgc actgtttgac cataaatgtg
     6541 ccgttttgcc gtatgtccgg ttgtaaactt tctccgtatt ttccgtcgtg gctcaacaca
     6601 tgacccaagc cgcgcgggaa aaatacaata tcgcccgtgc cgaccggacg cggggaagtt
     6661 tcgccgtcga tgcagagata accgctgccc gccgtaacaa tgtgtaccag cccttcgcat
     6721 tgcaaggttt catgccgtac cgaccattgt ccgcccaaaa ggcactgcac atccgcactg
     6781 cccgtcaatt gggcgagatc gaccagtttg tccagaatgt ccataaatct ttttgaacca
     6841 taaaatgaga tgattaaacg agaaacacaa ctgaataatt gcaataatac gcacatcaaa
     6901 aacagatacg caagcgcgta tcagggttaa acgcaagaga aaggaaaaga aaatgtttaa
     6961 agattggaaa gaacataccg cattggttaa aaaatcattc ggcgagctgg gtaaggcgca
     7021 tcctaaaatg ctgcaagcct acggcgcatt ggaacaggcc gccgccgccg aagcactcga
     7081 tgccaaaacg cgcgaactga ttgccatcgc cgttgccatc accacccgtt gcgagagctg
     7141 catcagcgtt cacgccgccg ccgccgccaa agccggtgcg accgacagcg agatcgcagg
     7201 tgcattggca accgccattg ccctgaatgc gggtgctgct tacacttacg ccctgcgtgc
     7261 attggaagcg gttgaaacgc aaaaataatc cgtttcggat aagaaatgcc gtctgaaaat
     7321 atttcagacg gcattttgcc tgttatgctt cccgatattt tgcagacaac atatgcagcc
     7381 gtttttttca gacggcattt gagaaacatg gaacaagtat gaaatatcac cgcttggcat
     7441 tgtttgccgc cataagctgc ctgttgttgt ccgccgtttt tattgcacct tacctgacgg
     7501 catttcacga acaagaaaaa atatttgaat atgcggattt gaccgttacc gcccccaacc
     7561 gcagcggacg ggcaatcaaa ttggaggcgg acgggcggca gtaccggctt tcctgctacg
     7621 ggttcgacag tttgtgcaca ggcggcaata tcggcagagc catcagggcg cggcaggtta
     7681 agattgtttt aagcgaaacc gtcggcaaag gttttttaaa cggcgttctg ttggaatacc
     7741 gcaacagtgg cagtgtctat agcaataaag atttttcccg cacggaagac cgccttgtcg
     7801 aagtgttggc acaacctgcc gttttcagcc tgaaaccggg tattttgctt ttattgcccg
     7861 ccattttcct gcgtttgaaa aaaatgtgaa aacagattgg cggggcgggg gaatgactat
     7921 gcggcaattt tacgttttgg gttatgagga taaccgttat aatcataatt ttacagtccg
     7981 cacagagaaa accgatgcct tggaacatcc ccatcttcct cacatggttg agggtcttgc
     8041 tcattcccgt actgattgtc cttttctacc tgccgttctc atggttttca gaagaagcag
     8101 tcaatgttgc cgccgccgtc atttttgccg ttgccgcctt gaccgactgg tttgacggat
     8161 ttttggcaag gttgtggaaa cagacttccg atttcggcgc attcctcgac cccgtcgccg
     8221 acaagctgat ggttgccgta tcgctgctgc tgctggtcaa actcgaccgg acctatgttt
     8281 tgttcgcgat gattatcatc ggcagggaaa ttaccatttc cgcattgcgc gaatggatgg
     8341 cgcaaatggg aaaaaggagc agcgttgccg tcgccaccgt cggcaagttc aaaaccgccg
     8401 cgcaaatgct ggcgattttc tttttgctgc tgaattttcc cgatttttac ggatttaatc
     8461 ttgttgttat cggcaacata ttgatgttta tcgcatcttt gctgacagtc tggtcgatgc
     8521 tgtattatct gaaaatggcg tggaaagaaa ttgcctgaaa aaaacataaa aatagcttga
     8581 cggtaaaaac ataatccata ataattgcgt cttcttcgat gtcggagagt gaaatcgggc
     8641 gggaatagct cagttggtag agcgcaacct tgccaaggtt gaggtcgcga gttcgagact
     8701 cgtttcccgc tccaaaaatt tcattcttcc gtaatcattg cgggaatagc tcagttggta
     8761 gagcgcaacc ttgccaaggt tgaggtcgcg agttcgaggc tcgtttcccg ctccaaaaat
     8821 ttcattcttc cgtaatcatt gcgggaatag ctcagttggt agagcgcaac cttgccaagg
     8881 ttgaggtcgc gagttcgaga ctcgtttccc gctccaagtt ttgtagacaa gccagtttta
     8941 gggcgagata gcaaagtggt tatgcagcgg attgcaaatc cgtctacgcc ggttcgattc
     9001 cgactctcgc ctccatatat cttacggcgg ggtggcagag tgtttatgct atgaggggtg
     9061 caaccttcat ataggccggt taaaatccgc gcccccgcct ccacctttca caaatgcccg
     9121 ggtggtgaaa taggtagaca caacggactt aaaatccgtc gggactaaac atcccatgcc
     9181 ggttcgattc cggctccggg caccaagctg aaaatgacaa tgccgctcca agcggttatt
     9241 tttttatctg tcggatgggg atggacaata ttgatatgtt catgcctgaa caagaggaaa
     9301 tccaatcaat gtggaaagaa attttactga attacggtat tttcctgctc gaactgctta
     9361 ccgtgttcgg cgcaattgcg ctgattgtgt tggctatcgt acagagtaag aaacagtcgg
     9421 aaagcggcag tgtcgtactg acagattttt cggaaaatta taaaaaacag cggcaatcgt
     9481 ttgaaacatt ctttttaagc gaggaagaga caaaacatca ggaaaaaaaa gaaaagaaaa
     9541 aggaaaaggc ggaagccaaa gcagagaaaa agcgtttgaa ggagggcggg gagaaatctg
     9601 ccgaaacgca aaaatcccgc ctttttgtgt tggattttga cggcgatttg tatgcacacg
     9661 ccgtagaatc cttgcgtcat gagattacgg cggtgctttt gattgccaag cctgaagatg
     9721 aggttctgct cagattggaa agtccgggcg gcgtggttca cggttacggt ttggcggctt
     9781 cgcagcttag gcgtttgcgc gaacgcaata ttccgctgac cgtcgccgtc gataaggtcg
     9841 cggcaagcgg cggctatatg atggcgtgtg tggcggataa aattgtttcc gctccgtttg
     9901 cgattatcgg ttcggtgggt gtggtggcgg aagtgccgaa tatccaccgc ctgttgaaaa
     9961 aacatgatat tgatgtggat gtgatgacgg cgggcgaatt taagcgcacg gttactttta
    10021 tgggtgaaaa tacggaaaag ggcaaacaga aattccggca ggaactggag gaaacgcatc
    10081 agttgttcaa gcagtttgtc agtgaaaacc gccccgggtt ggatattgaa aaaatagcga
    10141 cgggcgagca ttggttcggc cggcaggcgt tggcgttgaa cttgattgac gagatttcga
    10201 ccagtgatga tttgttgttg aaagcgtttg aaaacaaaca ggttatcgaa gtgaaatatc
    10261 aggagaagcg aagcctgatc cagcgcattg gtttgcaggc ggaagcttcc gttgaaaagt
    10321 tgtttgccaa acttgtcaac cggcgagcgg atgtgatgta gtttgccgaa cggatggaaa
    10381 tgccgtctga agtgtgttca gacggcattt ttcaagttcc ggctttgatg ttccgttccg
    10441 accactctat ccgttgtccg atgtctttca aattggaata ttgttcgacc aatcgcggca
    10501 catcgtccaa actcatggca aacatccaca gataagtgcc gaccgaataa atatgccccg
    10561 cgctgccgta gcctttgagc gtcatcatca caaaagcaaa gccgaacaaa atacccatcg
    10621 ccgcgccgac gcagagatag ccgaaggctt cgcggttgga aatcagcaca cgcaggcgcg
    10681 aaaccagtcc gtaatggcgg tacagctgcc gctcgtcgcc ttttcggata aagtggttgt
    10741 cgcgttccaa gctgttgttc aggcggaaat acaggttttc gctgatggcg gcaaaacgtg
    10801 gcaaaagcca taaaaacaac gcaagtatgc ccaccgccga cacgccgacc caaaattcca
    10861 gcaccagcag catgatgcac gcgccgaata tggatacgac ggatgtcgcg gcaatcggca
    10921 ggtgttcttc aaaaaagctg acaaattcac gcgacagggc aacccgtgca gttaccgctg
    10981 aatgcgggac ttgccgctgc cgttgttcca acacaaccgg cacggcgatt tcggtataaa
    11041 tccgcgtaaa cgtgcgcgta tcggcaatcc gccgtgccgc accgaccagc cacatcaaaa
    11101 atacaaccaa agcgtacagc aacgcctgcc acaccctccc cgcaatcacg gcattaatcg
    11161 cccagccgcc aaacaccgga taccccagca tcaaaaggtt ttccagtccg accggggaaa
    11221 atgtgccaat caatcgcttg cggtgggttt tggctatgtg tttcaacatt ttccacatat
    11281 ttgtccctct attttttatt ttccgagatt gttttttcca tcacttccgc cagtgcatcc
    11341 aaatcggcaa acagccgggt tgtgcgcttg tcgccgaatg tggaaaatac tttgtcgctg
    11401 aattcctgcg cgttttctgt taaaggtgcg gcatggactt tgcctttttc ggtcagcgac
    11461 agcaaccgtt cgcgcctgtc ctgttcgcct tcctgccatt caatcaaccc ttgtccggca
    11521 agggttttgc atacgcctga aacggtctgt ttgggcagac tccactcttc tccgatgtgc
    11581 ttttgcgtgc ggctgccttc ggttgccagg gtataaagta cggtaaagag gttgtaattc
    11641 aaatcctgct gcccgatcca tttgtcaaag acattgcaaa tcaggttgat acggattcca
    11701 agttggtcga gtcggttcat aattggtctt gatattgact aaatttgtgc ggattatagt
    11761 ggattaacaa aaaccggtac ggcgttgcct cgccttgtcg tactatttgt actgtctgcg
    11821 gcttcgtcgc cttgtcctga tttttgttaa tccactatat agtcataatc gggactgatt
    11881 tcaagcgtgg ggcgataaaa atgccgtctg aaacgctttc tggcttcaga cggcatattg
    11941 tttggaaagg gaagtcagtg ttctgccaat ttcaggtaaa cgccggaaac gtcctgttcg
    12001 ccgtagccgg cttcaactgc tttgcggtag ctggcagcaa cggtttcgac ggcgggcagg
    12061 gtgttgcctg cctgttcaag ctctttgacg gcgaggttaa ggtctttgga agcgtgtttg
    12121 agtgcaaagg caggggggaa ctcacggttt gcccatagtg atttttttgt ttgaaacata
    12181 ggcgagtcca ttgccgagcc gccgatggct tcgacgatgg tgtcggtatc gatgccgaac
    12241 tgccgcgcca tcagcatcgc ttcgctgtac gcttcgccga aaatgcctaa gagcgagttc
    12301 aagacgagtt tcgcgcccga gcctttgccg acatcgccga aatggaaggt ttttttgccg
    12361 acaagggaaa atattttttg cagcgggttt aaaacggctt cgctgccgcc gaacagaatc
    12421 agcagtgtgc cgttggtggc gggtccgacc gatccggaaa cgggtgcttc ggcaaactgt
    12481 ccgcctgcgg cttcgacaag tgctttgacg gcgaggtttt cggtcgggga gatggtgctc
    12541 atgttgacga tgattttgcc ggccaatccg tcgcggactc cgttcaggat gtcgcacacg
    12601 gcggcatagt cggaaaccat caggaaaatg acgggacagg cgcggacgag ttcggcggtg
    12661 ctgccgtaaa cttttgctcc tttggcggag atgggggcag ttttgtcggg cgagcggttg
    12721 tatacgccga cttcgatgcc gccgtccaag agccgcgtta ccataggcag acccatttgc
    12781 cctaagccta cccagccgat ttgtgtgtat gtttctgcgg acatagtgtt tctcctttgt
    12841 tggggcgctg taatgccgtc tgaaggcttc ctgcgcttca gacggtctgt ttggggatgt
    12901 tatgcggtca gcgtgccttt ggtcgagggg gtttgcccag ccattctcgg gtcgtgttcg
    12961 actgccatac gcagggcgcg cccgaaggct ttgaatacgg tttccgcctg atggtgggcg
    13021 tttttgccgc tgaggttgtc gatgtgcagg gtcatcatgc tgtggttgac gatgccgtgg
    13081 aaaaattctt caaacaaatc gacatcgaaa cgtccgatta gtgcgcgggt aaattcgatg
    13141 ttgtacacga gtccggggcg gccggaaagg tcgatgacga cgcggctgag ggcttcgtcg
    13201 agcgggacgt aggaatgtcc gtaacggcgg atgccttttt tgtcgccgag tgcctgccgg
    13261 attgcttgtc cgagtgtgat gccgatgtct tcggcggtgt ggtggtcgtc gatgtgcagg
    13321 tcgcctttgc agctgatgtc gatgtcaatc atgccgtggc gggcgatttg gtcgatcatg
    13381 tgttcgagga agggaacgcc ggtatccagc ctgcttttgc cgctgccgtc gaggttgatg
    13441 gagacggtga tttgggtttc gcaggtattg cggttgacgg taacgctgcg gcagccggca
    13501 gcagatgcgg tttcggcaat tcgggtttcg gcagttccgg tgctttctgc ggcggtttcg
    13561 gggacagtgc gttcgcggtg ttttctgtcg agccagcctt tgggtttgcc ggtgtgtttt
    13621 tcgagttttg ccatcaggct gggacggatg ccgcgcgcat cggggtcttc tgcctgcttt
    13681 tcaaggcgtt gtttgagttt gtaaagtgcg acgggggtac ggtagccgca gagtttggca
    13741 agcttgggca gcgaacctgc ttcttgggcg agagtcagga agttgttcag gtggagttgt
    13801 gtcttggtca tagggattcc gtttggttta tcggtagagc cggcggataa cgtcgaggac
    13861 ggcatcgttt tgtgcggagc tgcctatggt aatgcgcagg cagtgttcca aaagcggatg
    13921 tgcgccgtgc agttttttga cgaggatgcg gttttgtttg agtgtgtcaa acaacaaatc
    13981 ggcatcgggt acgcgtatgg taatgaagtt tgcctgactt ggaaaggcgt tcagacggca
    14041 tattttgccc aattcggcga acatccgttc gcgttcgttt ttcaggctgt cgatgttggc
    14101 agaaatgatg ccgtagtgtt gcagggcgag tttggcggtg gtcaggctca gttggttcat
    14161 attgtagggc ggcaggattt tttgcagttc gccgatgact tcgggacagc ctgtcgcata
    14221 accgatacgc agtccggcaa aaccgatttt gctgagggtg cgtaagacta tcaggttggg
    14281 aatcctgccc gcctgcggca ggaagctgtc gccgttgaat gcgccgtagg cttcgtcgac
    14341 gacgacgatg ccgtctgaag cttcgatggc ggcttcgatt tcggcacgcg tgaaacatac
    14401 gccggtcggg ttgttcgggt aggcgataaa ggtcagggca gggcggtgct tcctgacggc
    14461 ttcgaggacg gcgggcaggt tgagggtgaa atctccgttc agtggaacgc cgacataatc
    14521 catgccgtac agcgcggcgt tgtggcggta catgatgaaa ccgggttcgg ctgccaacat
    14581 tgccgcgccg ggtttggcgg tcagcatggt gatgaactgt atcagttcgt ccgaaccgtt
    14641 gcctaaggcg acggcggcgc agtcagggat gtcgaacgcc gaacgcaatg cttcctgcaa
    14701 accgcagccg gaggggttgg gatagaggtg gatgggggcg gatgccagtt gcgcccgcca
    14761 ttcccgcatc agggcttcgt gcccctcaaa aggatgggcg gggctttcca tcgcatcgag
    14821 tttggcaaaa ccgggcggaa catcggtgat tttgtatgcg gacatggcga ggatgtcgtt
    14881 gcggatgaag gagcggacgg atttcatcgt gtttccttaa tggttggaat atgcctgtac
    14941 gccgtttcgg cattatttca tacggaactc tgccgcgcgt gcgtgggcgg tcaggctttc
    15001 gccgtgtgcc agcacgctgg cggtttcgcc taatttttgc gcgccctgtt ccgaaacctg
    15061 aatcaggctg gagcgttttt ggaaatcata tgtccccaaa ggcgaggaaa agcgggcggt
    15121 tcggctggtg ggcaacacat ggttcgggcc ggcgcagtag tcgccgaggc tttcgccggt
    15181 gtagcgtccc atgaaaatcg cgccggcatg gcggattttt ttcgcccatt cctgcgggtt
    15241 ttcgactgac agttccaagt gttcggggga aatgtagttg gagatttcgc aggcttcgtt
    15301 caagtcttta acgagtatca tcgcgcccct gttgccgagc gaggcctcga tgatgtcgcg
    15361 gcgcggcatg gtttcgatga gcctgtccat ggcggcttct acttcgtcga gatacgcttg
    15421 cgacgtgccg atgaggatgg cttgggcaat ttcgtcgtgt tcggcctggc tgaacaaatc
    15481 catcgccacc caatcggcgg gtgtcgtgcc gtcggcgatg accagtattt cagacggccc
    15541 cgccaccatg tcgatgccga ccacgccgaa cacgcggcgt ttggcggcgg cgacgaaggc
    15601 gttgcccgga ccggtgattt tatcgacttg ggggatggtt tccgtgccgt aggcgagggc
    15661 ggcgatggct tgcgcgccgc cgacggtgaa gactttggtt acgccggcga cgtatgcggc
    15721 ggcaagcacg atgtcgttgc gttcgccttt cggtgtcggt acgaccataa tgatttcttt
    15781 cacgcctgcg acgtgggcgg gcatcgcgtt catgatgacg gaactcggat atgccgcctt
    15841 gccgcctggg acgtaaatgc cgacgcggtc aagcggggta atctgttgcc ccagcagcgt
    15901 gccgtcttca tcggtgtagc tccacgattc cattttttgg cgttggtggt agctttcgac
    15961 acggcgggcg gcggtctgca atgctgtctg aacgtcgttc ggaatgcgct cgaacgccgc
    16021 gttcaaatcg gcttgcgtga gtattaaatc atcgatgctt ttagcgtttg tctgatcgaa
    16081 tttgttggtg tattcaatca aagccgcatc gccgcgcttt tgcacgtcgg cgcaaatgtc
    16141 ggcgacgatg cgttcggttt cggggttttg cgcggtttca aaagccagca gggctttgag
    16201 tccggcttgg aaatcgggcg attgggtgtt gagttttttc atgatttggg attctccttt
    16261 gttattttgg gacttgggat cgtctgaagc gtctcttgct cggttttggg tctgatatac
    16321 gcttttcctc ccccgctacc aaacttcaat tcgggcaggc atttttgttg cagaccgagt
    16381 ttggctaata cctcttcgct acgagaggta gtacggaaac ggtaacgggt ttgttttctg
    16441 ccgtttttgg tttcctcagt cattgtttca aaaccgtcca cttcgtacgc actgacaaca
    16501 gcgttttgca gaccggtgtg aacgccgata acgtatttca cttttgaagc aacatcttta
    16561 ccgataaccc agttgcccaa cgtacgcgat ttgaggttgg catcgtcttg gttgtcgaaa
    16621 aggtagtcta attcttcgtc agtatctaaa tcgaaagcat tgtggatctt gatggcgaga
    16681 atcagcccgt cggggttaat ctcgctaagt gggagagaag agaatccaaa gcggcgatct
    16741 agttcttcta cgctaatacc acccggacca tgcccggcaa ttttattttt gagtttttta
    16801 cccaagacag atttaacaaa atgaattaag gcagattcgg cggcgagcgc ttcgacttca
    16861 gtcagatgat agctgatgat atagcgaccg agtttcttgc atttggagat ggctttgagt
    16921 ttccgatcga taatctcgcc ggagactggg tcttgtgaac gactagcaac ccattcatgc
    16981 tcgaagatac gattaccgca accttttccg atgtagagga ttttgtcttt tttcaggtcg
    17041 gtcagacaat agacataaaa ccgacgctcg ccgttattca aaacggaaag ggtcgaatct
    17101 gagaattttt ttatttttgc aaccatttta acttctccta tacagtttac tgccggtgat
    17161 attttcagac ggcctgtcgg cgggaacgga tagggtcgtc tgaaaacgca tctttattca
    17221 aatgaatgcc cacttcgcct tcactgcgcc gccgaacgac tgaataatcg gctccagcag
    17281 cgcgtatttc gttttcaaag cagccttgtt gaccaccagg tagctggaaa tgtcgacgat
    17341 gtgttcgact gcctccaagc cgtttgcctt caaggtgttt cccgtcgaaa ccaagtccac
    17401 aatcgcatcg ctcaagccga ccagcggcgc aagttccatc gagccgtaca gtttgataat
    17461 gtccacatgg acacccttgc cggcaaaatg agatgccgcg atttcaggat acttcgtggc
    17521 aatcttcaga cggcatccgg gttgcgaagc ggcttcgtaa tcaaaccctt tacgcacagc
    17581 aaccatcatg cggcacttgg caatctccaa atccaaaggc cggtaaagcc ccgtgccgcc
    17641 gtgttcgatc agcacgtctt tgcccgcaat gccgaagtcc gccgcgccgt agcggacata
    17701 agtcggcaca tcggttgcgc ggacaatgac aaggcggatg ttttcatggt tcgtcccgat
    17761 aatcagcttg cgcgattttt cgggctcttc agtcggagca atgccggcag cggcaagcag
    17821 cggcagcgtc tcctcaaaaa tgcgcccttt ggataaggcg atggtcaaag cattatcctg
    17881 catggtgtgg atgtcctttg tcggttgaaa caataatgcc gtctgaacag gcgccggcgg
    17941 tgcagacggc atacggtttt gtcagagcag ggtccttaca tcggcagcaa tcgtttccgg
    18001 ctcgcttccg taaggcgaga aaatggcaac ctcaccgttt ttgtcgatga gatacgcacc
    18061 ggaagagtgg tcgaccaaat agttttcgct gtcgtctttt tgattgattt tggcagaaac
    18121 cacgcggtat tgctgcttga tgaccggcag gttttgaccg cccgttgccg tcagaccgat
    18181 aaagtccgga ttgaactgtt tggcatactt gccgatgatt tcaggcgtgt cgcgttccgg
    18241 atcgatgctg acgaacacca ctttcacgtc cttagcctgc ccgcccaact gcttcaaagt
    18301 gtcgctgtac gtcaaaagct ctgtcgggca gacatcggga cagtgcgtaa agccgaaaga
    18361 cagaatcacg accttgcctt tcaaatcgct caggctgaaa ggcttgcctt cgccgtcggt
    18421 cagcgtgaaa tcgccgccga tgtcttcctt acgcatatcc gtaccgcgcg tttgcggctt
    18481 tgccgcattt tccgcagccg gcgcggatgc acttgaagaa gcggcttgcg ccgcactgtt
    18541 gtcttgaggt ttgcaggcgg caagcgcggc aagtacgaaa acgcccggca aaaaggaacg
    18601 cggtacggaa aacatgacaa tctcccaaga ataaatagaa aaacctgatt cgtacggaat
    18661 ttgaatattt ccatcttaac gcgtttggca gatttaatgt agtatttttt tacgttgtcc
    18721 gtacggcagg aattgccgaa tcatacagaa tctttggagc ggaaagaaca gtaagattaa
    18781 aataataaaa attctattta aaatcaaaaa caaaataaaa acagataaaa taatttcaaa
    18841 aaaaggttta cagaatctca aaatccatta taatggcaat ttcttcacca gcccaggtgg
    18901 cgaaattggt agacgcaggg gactcaaaat cccccgccgc aaggtgtgtc ggttcgagtc
    18961 cgaccctggg caccacaacc gcttttggag cggttatttt tttgtctatt gctgtttaat
    19021 gaaatagata agactaaata tagtggatta aatttaaacc ggtacggcgt tgcctcgcct
    19081 tgccgtacca tctgtactgt ctgcggcttc gtcgttttgt cctgatttaa atttaatcca
    19141 ctatacaatc aatatattta agtcgttgtt agccctcttg gttctgtcaa tgccgtccgg
    19201 tgcaatgagc cattttcagg aagtatggtt gggaatattt ttgatatatc aaaaataaaa
    19261 tcaagttcaa tcaatatccc gagtgaaacc ctgtaaattt taaaattctt gatactttca
    19321 catctgcccc catgcaaact gtcaacaatt tccatctttc aaaaaaggta ctcctgattt
    19381 tattctttta aaatcaatca ataatttctc cgctttcaaa aaaatttctc cgctttcaaa
    19441 aaatattaaa agatgaaaga tttttcttga ttgaactgtc ggtttgcgtt atcgttttta
    19501 ctctttttca caaactctgt gttcctttcc aattgattgg ataatctgtc tgttgccgtg
    19561 ttcccccaat gcgcgtaccc taaatcgctg cttggtggaa ttgcattaca ggtgctgtgg
    19621 caaagcggtt tgtccctatt ggtttgaaac cgtataaaag aggtcgttat gcagttatca
    19681 ggcgcgcaaa tcatagtgca gagtctcaaa gccgaaggcg tcgagtacgt tttcggttat
    19741 cccggcggtg cggttatcga aatctacgat gccctgttcc aactcaataa attcaaacac
    19801 attcttaccc gccatgagca ggcggcagta cacgcggcag atgcgtatgc gcgcgtcagc
    19861 ggtaaggtgg gcgtggcatt ggttacatcc ggcccgggcg ttaccaatgc gctgaccggt
    19921 attgctactg cctatacgga ttcgattccg atggtggtca tcagcggaca ggtaggcaat
    19981 tccctgattg gtacggatgc gttccaagaa gttgatacgg tgggtattac ccgtccgtgc
    20041 gtcaaacaca atttcttggt tacggacatc aatgagttgg tggaaaccat taaaaaggcg
    20101 ttccaaattg ccgcaagcgg ccgaccgggg cccgtggtgc ttgatgtccc gaaagatgtt
    20161 acgcaggcga tggcgaaatt cagctatcct caggaagaca tttttatccg ttcgtatcaa
    20221 ccggttgttc aagggcatat cggtcagatt aaaaaggccg tgcagatgtt ggcatctgcc
    20281 aaacgcccgg tcgtctattt tggcggcggc gtggtgttgg gtaatgcttc tgaagagctg
    20341 acccgatttg tccgaatgac gggtgctccg tgcacgggta cgctgatggg cttgggcgct
    20401 tatccttccg gcgaccgcca attcctcggt atgctcggta tgcacggtac ttacgaggca
    20461 aaccttgcca tgcagaatgc ggatgtcgtt cttgccgtag gtgcgcgttt tgacgaccgt
    20521 gtggtttccg taccgtccaa atttttcgag aaggcgaaga aggttatcca tatcgatgtc
    20581 gatccttcca gcatcgccaa acgcgtgaag gcggacattc cgattgtcgg cgacgtgaaa
    20641 aacattttgt ccgagatggt tgcgctgtgg caaaaacaag agtccgtgcc gtctgaagat
    20701 gctttgggca aatggtggaa aaccatagag gaatggcgtt cccgcgattg cttgtggttt
    20761 gacaacggca gcgaaattat caagccccaa tatgtgattc agaagcttgc cgagattacc
    20821 ggcaattcgg caatcatcac atcggatgta gggcagcatc aaatgtttgc ggctcaatat
    20881 tatcctttcg aacgtccgcg ccaatggctc aactccggcg gtttgggtac gatgggcgtg
    20941 ggtctgcctt atgcgattgg tgcaaaactt gccgccccgg atcaagacgt attctgtatt
    21001 accggcgacg gctcgatcca gatgaacatc caagagttgt ccacctgttt ccaatatcgg
    21061 attccggtta acgtcattac gctgaacaac ggttatctgg gtatggtacg ccagtggcag
    21121 gaaatatatt acggcggtcg agagtcggaa acctatttcg attctttgcc cgatttcgtc
    21181 aaacttgccg aggcatacgg ccatatcggc atccgcgtgg acaagaaatc tgatgtggaa
    21241 ggtgccttgt tggaagcatt gaaccaaaaa gacaggctgg tgtttatcga cttcctgacc
    21301 gaccagaaac agaatgtgat gcctatggtc ggcaacggca aaggtttgga cgaaatggta
    21361 cttccgccgc atatgcgtac ggacggaaag gcgtaaggag aggcaaatgc gacatatctt
    21421 atctgttctg atcgaaaacg aatcaggtgc gatgagccgc gtggtcggtt tgttctctgc
    21481 acgcgattac aatatcgatt ctttggcggt tgcgccgacc gaagacaaaa ccctgtcacg
    21541 gatgaccatc gttacccacg gcgacgagca ggttatcgaa caaatcacca agcaactcaa
    21601 taaattgatt gaagtgatta aagtggtcga tttgaacgaa agccgttttg tcgaacgcga
    21661 actgatgttg gtaaaagtcc gtgccgccgg caaagaccgc gacgaatttt tacgcttgac
    21721 cgaaatctac cggggcagca tcatcgacgt aaccgaccgc agctatacga ttgaaattac
    21781 cggctcgacc gacaagctgg attccttttt ggaaacggtc ggacgcgccc aaattttgga
    21841 aaccgtacgt acaggtgcgg ccggcatcgg tcgcggtgag cgtattttga aaatttaacg
    21901 ccgtaacctt tcagacggca tggtatttga atgccgtctg aaaaacgaac ggcaggagag
    21961 atttatgtcg aacattaaaa ttgtcgcact ggttaccgtc aaaccggaat acacggaaac
    22021 actggcagca cagtttaaag aactggtcaa agccagccgt gcggaagagg gcaacatcag
    22081 ctacaatctc catcaggaaa tcggcaaacc gaaccgtttt gttttcgtgg aaaattggaa
    22141 atcccaagca gctattgacg aacataatgc cagcgcgcat ttccaagcct tcgtccagtc
    22201 cgtcgacggc aaaaccgaag cgttggaaat cgtattgatg aatgaagttg ccgtttaagc
    22261 ggcacactct gtttaacccg tccgaagccg ctcaacattt taaggcttcg acaaccattt
    22321 accttaaagg aaatcaaatg caagtctatt acgataaaga tgccgatctg tccctgatca
    22381 aaggcaaaac cgttgccatc atcggttacg gttcgcaagg tcatgcccat gctgccaacc
    22441 tgaaagattc gggtgtaaac gtggtgatcg gtctgcgcca cggctcttct tggaaaaaag
    22501 ccgaagcagc cggccatgtt gttaaaaccg ttgctgaagc gaccaaagaa gccgatgtcg
    22561 ttatgctgct gctgcctgac gaaaccatgc ctgccgtcta tcacgccgaa gttgcagcca
    22621 atttgaaaga aggcgcgacg ctggcatttg cacacggctt caacgtgcac tacaaccaaa
    22681 tcgttccgcg tgccgacttg gacgtgatta tggttgcccc caaaggtccg ggtcataccg
    22741 tacgcagtga atacaaacgc ggcggcggcg tgccttctct gattgccgtt taccaagaca
    22801 attccggcaa ggccaaagac atcgccctgt cttatgcggc tgccaacggc ggcaccaaag
    22861 gcggtgtgat tgaaaccact ttccgcgaag aaaccgaaac cgatctgttc ggcgaacaag
    22921 ccgtattgtg cggtggcgtg gccgagttga tcaaagcagg ttttgaaacc ctgaccgaag
    22981 ccggttacgc gcctgaaatg gcttacttcg aatgcctgca cgaaatgaaa ctgatcgttg
    23041 acctgatttt cgaaggcggt attgccaata tgaactactc catttccaac aatgcggagt
    23101 acggcgaata cgttaccggc cctgaagtgg tcaatgcttc cagcaaagaa gccatgcgca
    23161 atgccctgaa acgcatccaa accggcgaat acgcaaaaat gtttatccaa gagggtaatg
    23221 tcaactacgc gtctatgact gcccgccgcc gtttgaatgc cgaccaccaa gttgaaaaag
    23281 tcggcgcacg actgcgtgcc atgatgcctt ggattacggc caacaaattg gttgaccaag
    23341 acaaaaactg attgttttca aacgggactg cctacacatc gtgtaggcag tttgttatat
    23401 ggataccgtc tgaacatcgt gttcggacgg tatctgtgtt gcggatgaat ttaaacaggc
    23461 acagttctgt cagtcgccca gagccgcgac cattaccgct ttaatcgtgt gcatacggtt
    23521 ttccgcctga tcgaacacga tgccggccgg actttcgaat acttcttctg taacttccac
    23581 accgttcagc ccgaaggttt cgtaaatcca ttcgccgact ttggtttcgc ggttgtggaa
    23641 ggcgggcagg cagtgcatga atttgacttg cggattgccc gatgccgcca tcagttcggg
    23701 cgtaacgcgg taatctttca gcaaatcgat gcgttcctgc cagacttctt tcggctcgcc
    23761 catgctgacc catacgtcag tatgaatgaa accgacacct ttgacggctt catgcgcgtt
    23821 ttcggtcagg gtaatttttg caccggtttc tttggcggcg gcgtgtgcgg cggcgataat
    23881 gccttcagac ggccacaggc tttgaggtgc gccgatacgc acgtccatcc ccaattttgc
    23941 ccctaaaatc agcagggaat tgcccatgtt gtaacgcgcg tcgccgacgt aggcaaacgc
    24001 ggtttggttc aaaggtttgc cgctgtgttc gcgcatagtc agtgcgtcgg caagcatttg
    24061 tgtgggatgg aactcgttgg tcagcccgtt gaacacgggt acgcccgcat attttgccaa
    24121 ttcttcgaca gtttcctgag cgaagccgcg atattcgatg gcatcgtaca ttctgcctaa
    24181 gacgcgcgcc gtgtctttga tgctttcttt gtgcccgatt tggctggcgg acggttccag
    24241 ataggttgca tccgcgcctt ggtcgcgtgc ggcgacttca aacgcacagc gtgtgcgcgt
    24301 ggatgttttt tcaaaaatca gggcgatgtt tttccctttc atccgctgaa tctcgcgccc
    24361 tgcctttttg gcgtctttca actcggcggc aaggtcgagg taggtggtga tttcttccgg
    24421 cgtgaagtcc aaaagtttca gaaaatggcg gtttttcagg ttcactgtcg tttcctttat
    24481 gtgggggcgt gaacgccgta tttgcagcgt ttgcggaaag aagggttagc ggaatgttgc
    24541 aatgccgtct gaaggttgtt cagacggcat tgcggtcaat cccttactcc atacggatga
    24601 cggggatcaa acattcacgg tctcggctac ttcgttgtag ctgtcgattt cgttgaagtt
    24661 catatagcgg tagattttat cgccctgttc gttgatgatg ccgatattgg cttgatattc
    24721 ttcaacggtc gggattttac ccagtttgga gcaaatcgcc gccaactctg ccgagccgag
    24781 gtaaacgaag gtgtttttac ccaagcggtt cgggaagttg cgggtcgaag tggacatgac
    24841 agttgcacct tcgcgtactt gggcttggtt acccatacac aacgaacagc ccggcatttc
    24901 catacgcgcg ccggcgcggc cgagtacgcc gtagtgacct tcgtcggaca actctttcgc
    24961 gtccattttg gtcggcggcg ctacccacag gcggacgggg atgtcgctct tgccttccaa
    25021 aagtttggag gcggcgcgga agtggccgat gttggtcata caagaaccga tgaacacttc
    25081 gtcgattttg gtgccggagc gttcggacat aaagcatacg tcgtccgggt cgttcgggca
    25141 ggcgataatc ggctctttga tgtcgtccat gttgatttca atcacggcgg cgtattcggc
    25201 atctttatcc gcttcgagca actcgggatt tgccagccat ttttccatag ctttgatgcg
    25261 gcgttccaaa gcgcgcggat cttgatagcc gttggcaatc atatttttca tcaacacgac
    25321 gttggatttc atgtactcga taatcggctc tttattgagc ttcacggtac agccggcggc
    25381 ggagcgttcg gcggatgcgt cggtcaattc aaaggcttgt tccactttca aatcgggcag
    25441 gccttcgatt tcgaggatgc ggccggagaa gatgtttttc ttaccggctt tggcaacggt
    25501 cagcaaacct tgtttaatcg cgtaaagcgg gatggcgttc accaaatcgc gcagggttac
    25561 gcccggttgc agcttgccgc tgaaacgtac caatacggac tcgggcatat cgagcggtat
    25621 tacgcccgtt gcggcggcaa aagcgaccaa gcctgaacct gcggggaagg aaataccgat
    25681 ggggaaacgg gtgtggctgt cgccgcctgt accgactgta tcaggcaaca gcaggcggtt
    25741 gagccacgag tggatgacgc cgtcgcccgg acgcagggac acgccgccac gggtagaaat
    25801 aaaggcgggc agttctttat gggtttttac atcgacaggt ttcggatagg cagcggtgtg
    25861 gcagaaagac tgcatcacca tatcggcgga gaagcccaaa caagccaagt ctttcaactc
    25921 gtcgcgggtc atcggcccgg tcgtatcttg cgagccgacc gtcgtcatgc gcggttcgca
    25981 gtaagtaccc ggacgcacgc cttgtccttc gggcagaccg caggcgcgac cgaccatttt
    26041 ttgcgccaag gtgaaaccga ctttgctttc ggcaggcgct tgcggcaggc ggaatgcagt
    26101 agaggcaggc agtttcaggg cttcgcgcgc tttggcggtc agaccgcgac cgataatcag
    26161 gttgatacgg ccgccggctt gcacttcgtc cagcaatact tgtgatttca attcaaactc
    26221 ggcaacagtc tcgccgtttt tcacgatttt accttcataa ggaaggatat tgacgacatc
    26281 gcccattttc agcgcggaaa catcgacttc aatcggcagc gcgccggagt cttcttgagt
    26341 gttgaagaaa atcggcgcga ttttaccgcc caagcacacg ccgccgaagc gtttgttcgg
    26401 cacgaacggg atgtcttcgc cggtatgcca aatgacggag ttggtcgcgg atttgcgtga
    26461 agaaccggta ccgaccacgt caccgacgta ggcaaccgga tggcctttgg ctttgagttc
    26521 ttccaacaat ttaatcggac cgacttcgcc cggtttgtcg ggcgtgatgc cgtcgcgcgg
    26581 gtttttcagc atggccagcg cgtgcagcgg aatatcggga cgactccacg cgtcgggcgc
    26641 gggagagagg tcgtctgtat tggtttcgcc gtcaactttg aagacggtaa cggtgatttt
    26701 ttcgggaact ttggcacggg aggcgaacca ttcggcatct gcccaagatt gcaaaacttc
    26761 ctgcgcgtat ttgttgcctt tttcggcttt ttcctgaacg tcgtggaagg aatcgaacat
    26821 cagaagcgta tgtttcaagc ctttggcggc aatgggcgcg agtttgtcgt cgtccaagag
    26881 ttcgattaag gcgtggatat tgtaaccgcc gagcatcgta cctaagagtt cggtcgcata
    26941 tttgggggaa accagcgggc tggatgcgct gccttcggca acggcagcca ggaatgaggc
    27001 tttgactttg gcggcatcgt ccacaccggg cggaacacgg tgggcaagca gctcgaccaa
    27061 gaactcgcct tcgcctgcgg gcgggttttt cagcagctca accaaatcgg cggtttgctg
    27121 cgcattcaaa gggagggcgg gaatgccgag ggcggcgcgc tcggcggcgg ctttacgata
    27181 ggcttctaac atctctttgt tcctttttcc gtttttcttt tgtcgggttg caaatgatct
    27241 gcgttaaata ttgtaaacaa catttgtacc gctatcatag actagtttta acaaaaatgg
    27301 aactgttatg acaggtaat
//
LOCUS       DBJGGI010000022        27208 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.022, whole genome shotgun sequence.
ACCESSION   DBJGGI010000022 DBJGGI010000000
VERSION     DBJGGI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27208
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.022"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(<1..210)
                     /locus_tag="ACSIGJ_000942"
     CDS             complement(<1..210)
                     /locus_tag="ACSIGJ_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HHQ1329834.1"
                     /translation="MRKSRLSQYKQNKLIELFVAGVTARTAAELVGVNKNTAAYYFHR
                     LRLLIYQNSPHLEMFDGEVEADESYF"
     gene            277..579
                     /locus_tag="ACSIGJ_000943"
     CDS             277..579
                     /locus_tag="ACSIGJ_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329835.1"
                     /translation="MKDFDEVCEKWEKSFKENGKEIEYNQKYSRIYGEVRGAGFNGDI
                     GEKLHRCAVMEWAENETVKLAQKWEQEQKKQQIQQKKETEKSPKHKASRDDWEMER"
     gene            complement(661..2748)
                     /locus_tag="ACSIGJ_000944"
     CDS             complement(661..2748)
                     /locus_tag="ACSIGJ_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HHQ1329836.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGIALELFPNTHFEEKKETKKIKILSLL
                     RGISGLKRSLIQMLILAISLEVFALVSPFFMQWVIDHIIVTADKNLLLTLTLGFGLLA
                     ILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSIDH
                     IQETLTSTFFVLVLNSLMAIFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLRNA
                     TEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLSAL
                     FEFSNKLLFSMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYIQI
                     KMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFENI
                     NLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGLSG
                     IVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDMGN
                     ILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAHRQ
                     ETIQSADKILNLG"
     gene            complement(2751..4016)
                     /locus_tag="ACSIGJ_000945"
     CDS             complement(2751..4016)
                     /locus_tag="ACSIGJ_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HHQ1329837.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKREQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKPKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            complement(4737..5018)
                     /locus_tag="ACSIGJ_000946"
     CDS             complement(4737..5018)
                     /locus_tag="ACSIGJ_000946"
                     /inference="COORDINATES: protein motif:HMM:NF045139.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6861 domain-containing protein"
                     /protein_id="HHQ1329838.1"
                     /translation="MSNDENLLFSQPHLTKVNIMQVLTLDEIQQVSGAACNWRDFSKN
                     TIGSALGGAAGGAIVGSFAGGIGAIPGAKFGAIGGAITGSVQYGSTCWW"
     gene            complement(5239..5475)
                     /locus_tag="ACSIGJ_000947"
     CDS             complement(5239..5475)
                     /locus_tag="ACSIGJ_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HHQ1329839.1"
                     /translation="MKELHTSELVEVSGGKFHISAQGGGNLGKKDMVAVGKIGVSYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGFSKKW"
     gene            complement(6431..6757)
                     /locus_tag="ACSIGJ_000948"
                     /pseudo
     CDS             complement(6431..6757)
                     /locus_tag="ACSIGJ_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            7017..8489
                     /gene="pyk"
                     /locus_tag="ACSIGJ_000949"
     CDS             7017..8489
                     /gene="pyk"
                     /locus_tag="ACSIGJ_000949"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HHQ1329840.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HILKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQTEYPEAVSTNLAIAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            8648..10087
                     /locus_tag="ACSIGJ_000950"
     CDS             8648..10087
                     /locus_tag="ACSIGJ_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HHQ1329841.1"
                     /translation="MPFLFVHRPPFYGNRNDPFHTEKTVLLLGTAFAAASVHASGYHF
                     GTQSVNAQSTANAAAAEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSAT
                     DFTGLPVQGSKSGKITKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRH
                     NINKLGLTSIAVEPVAAWKLNDRHSFGAGIIAQHTSAELRKYADWGIMEKAKALKETP
                     PNPTKAAQIKADGHADVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEW
                     AADGAAAKAMWNGNMLAPLGYTPSEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWT
                     RHSRFDKAELVFEKEKTIVNGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAF
                     DKSPVRNADYRMNSLPDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTTYRTAKASG
                     NDVDSKGASSARFKNHADIIGLQYTYKFK"
     gene            complement(10307..10696)
                     /locus_tag="ACSIGJ_000951"
     CDS             complement(10307..10696)
                     /locus_tag="ACSIGJ_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329842.1"
                     /translation="MEKYLEQFGPVSESIEACTAKLQEQPSFFNRLMKTNDKLNRQID
                     VLQKQSAAIHNEAYIEMNTLLYRHREVVSVHNRKADYAEKGKERIALFPRGLNGITKL
                     PAAVLLPERSDCFDMKAVWQMFNRIPR"
     gene            complement(10956..11105)
                     /locus_tag="ACSIGJ_000952"
     CDS             complement(10956..11105)
                     /locus_tag="ACSIGJ_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329843.1"
                     /translation="MPSEILLSVSDGILTNTVRRRKTAGFSCVAYADVPTHRTAHKAS
                     PYVPS"
     gene            complement(11123..11320)
                     /locus_tag="ACSIGJ_000953"
     CDS             complement(11123..11320)
                     /locus_tag="ACSIGJ_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329844.1"
                     /translation="MTTVPKSRIPVKPLPEKTTAEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            complement(11331..12347)
                     /locus_tag="ACSIGJ_000954"
     CDS             complement(11331..12347)
                     /locus_tag="ACSIGJ_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HHQ1329845.1"
                     /translation="MYRKLIALPFALLLAACGREETPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLMAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDADVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            complement(12446..13660)
                     /gene="gltS"
                     /locus_tag="ACSIGJ_000955"
     CDS             complement(12446..13660)
                     /gene="gltS"
                     /locus_tag="ACSIGJ_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HHQ1329846.1"
                     /translation="MEWAFNSYYTLIAATLVLLVGKVLVKKIKILRDFNIPEPVAGGL
                     IAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENTKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGSVTVILAVQTAVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            complement(14108..14206)
                     /locus_tag="ACSIGJ_000956"
                     /pseudo
     CDS             complement(14108..14206)
                     /locus_tag="ACSIGJ_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14213..15337)
                     /locus_tag="ACSIGJ_000957"
     CDS             complement(14213..15337)
                     /locus_tag="ACSIGJ_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HHQ1329847.1"
                     /translation="MSNNQPNSQIYEDYWAFTNAFTNYNDQKFCTALNICLDFIDANQ
                     DQPYSNELYEALQQNIAQDSVMASQRTSKAMNLASVRKAINQFVKMGFIEPFLSSYTP
                     LSRDYVQARTNRKRQTLLSKIVYTHSGFQRSVTENSNIRQINFLIKTLVEHPQGKLNK
                     KEIAAMMLVDLKTFQQDYLTETELNDYFQQGIESGFIERKYNQISYLWNLLDKLDDLK
                     RVGDDLYFAEDAQRIFGNLDEITVRKRDPYLHRLYKNQLQEESEEHYGNVKCMLEKLA
                     YPVLIASHIKPFILSDDTEAYDPNNGLLLSRTLDSLFDLKYISFDDEGNMVKSKRLSD
                     DVWRRWCDVKLDNNLLNDKRKSYLAYHRELMLQEDQEFHI"
     gene            complement(15400..16407)
                     /locus_tag="ACSIGJ_000958"
     CDS             complement(15400..16407)
                     /locus_tag="ACSIGJ_000958"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0032775 - obsolete DNA methylation on
                     adenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HHQ1329848.1"
                     /translation="MNKKYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYSEK
                     FYFHDECQQLDLYRKNIGKLNNQYKRALAFTLMRRAMIRKMPYSRFTIPWEKVKQLRD
                     EEYSYAKYGRRRAYHNESFQSHFLQNLDDYNQAVFNNGWRHHAFNQDIFDLLPNIQAD
                     AVYLDPPYTGTMNNYFGFYGLLDSYITSSIPKPFANHFMDKKQAVELFKRVINHLKPF
                     KYWLLSYNNASYPNRDELEEMLGKNGRNVQILETPHVYKVTGKEKKQSHKEILFLVEN
                     T"
     gene            complement(16400..17401)
                     /locus_tag="ACSIGJ_000959"
     CDS             complement(16400..17401)
                     /locus_tag="ACSIGJ_000959"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HHQ1329849.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPRHQLLQKDLTTLTEQDLTNCLNGQSVDLVIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGKTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKPAVCITGDMRKVFHY
                     EQNRALTVRELAALQSFPDNFIFCGSKIAQQQQVGNAVPPLLAKAIAESILKMSENE"
     gene            complement(17432..19705)
                     /locus_tag="ACSIGJ_000960"
     CDS             complement(17432..19705)
                     /locus_tag="ACSIGJ_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HHQ1329850.1"
                     /translation="MNITRILSQELSATTVQINAAIELLDDGATVPFIARYRKEATGG
                     LDDTQLRQLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQPQDVEAAAQGYLNENIPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHTQVVEGKETEGEKFSDYFDRREPVRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     GMPESSLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSRLAKSLDAVVEDCVNAVGVDANTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGITAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGAFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRD
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPTNSAMADAFAKLKR"
     gene            19820..20836
                     /gene="galE"
                     /locus_tag="ACSIGJ_000961"
     CDS             19820..20836
                     /gene="galE"
                     /locus_tag="ACSIGJ_000961"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HHQ1329851.1"
                     /translation="MTVLITGGTGFIGSHTAVSLVQSGYDAVILDNLCNSSAAVLPRL
                     RQITGRNIPFYQGDIRDCQILRQIFSEHEIESVIHFAGLKAVGESVAEPTKYYGNNVY
                     GSLVLAEEMARAGVLKIVFSSSATVYGDAEKVPYTEDMRPGDTANPYGASKAMVERML
                     TDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGVPNNLLPYICQVASGRLPQLSVF
                     GGDYPTPDGTGMRDYIHVMDLAEGHIAAMKAKGGVAGVHLFNLGSGRAYSVLEIIRAF
                     EAASGLHIPYRIQPRRAGDLACSYADPSHTKQQTGWETKRGLQQMMEDSWRWVSRNPG
                     RYGD"
     gene            20837..21877
                     /gene="rfbB"
                     /locus_tag="ACSIGJ_000962"
     CDS             20837..21877
                     /gene="rfbB"
                     /locus_tag="ACSIGJ_000962"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HHQ1329852.1"
                     /translation="MQTAGKKNILVTGGAGFIGSAVVRHIIQNTRDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQYRPDAVMHLAAESHVDRAIGSAGEF
                     IRTNIVGTFDLLEAARAYWQQMPSEKREAFRFHHISTDEVYGDLHGTDDLFTETTPYA
                     PSSPYSASKAAADHLVRAWQRTYRLPSIVSNCSNNYGPRQFPEKLIPLMILNALSGKP
                     LPVYGDGAQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKTNLEVIKTICALLE
                     ELAPEKPAGVARYEDLITFVQDRPGHDARYAVDAAKIRRDLGWLPLETFESGLRKTVQ
                     WYLDNKTRRQNA"
     gene            21982..22848
                     /gene="rfbA"
                     /locus_tag="ACSIGJ_000963"
     CDS             21982..22848
                     /gene="rfbA"
                     /locus_tag="ACSIGJ_000963"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HHQ1329853.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNAAFQRLLGDGSDFGIRLQYAVQPSPDGLAQAFIIGEEFIGNGNV
                     CLILGDNIFYGQSFTQTLKQAAAKTHGATVFGYRVKDPERFGVVEFDENFNALSIEEK
                     PQQPKSDWAVTGLYFHDNRAVEFAKQLKPSARGELEISDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTQESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLTKKDVETRAKPLEKT
                     AYGQYLLRLIGK"
     gene            22987..23544
                     /gene="rfbC"
                     /locus_tag="ACSIGJ_000964"
     CDS             22987..23544
                     /gene="rfbC"
                     /locus_tag="ACSIGJ_000964"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HHQ1329854.1"
                     /translation="MDIIDTALPDVKLLKPQVFTDGRGFFMETFRDGWFKENIADRTF
                     VQENHSNSSKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNRYQLWIPEGFAHGFCVLGDAAEVVYKCTDYYNPETEQVLIWNDPAIGIGWPLQTA
                     PLLSPKDLAGKTWAQAEKLRLTLSR"
     gene            complement(23588..25604)
                     /locus_tag="ACSIGJ_000965"
                     /pseudo
     CDS             complement(23588..25604)
                     /locus_tag="ACSIGJ_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
     gene            complement(25799..26920)
                     /gene="dnaJ"
                     /locus_tag="ACSIGJ_000966"
     CDS             complement(25799..26920)
                     /gene="dnaJ"
                     /locus_tag="ACSIGJ_000966"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HHQ1329855.1"
                     /translation="MSNQDFYATLGVARAATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGAS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCRGAGKHIKEPCVKCRGVGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            27054..>27208
                     /locus_tag="ACSIGJ_000967"
     CDS             27054..>27208
                     /locus_tag="ACSIGJ_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HHQ1329856.1"
                     /translation="MSTFFRQTAQAMTAKHIGRFPLLELDQVIDWQPIEQYLNRQKTR
                     YLRDRRGR"
BASE COUNT         7204 a         6873 c         6139 g         6992 t
ORIGIN      
        1 aaaataactt tcatctgctt ctacttcgcc atcaaacatt tccaaatgcg gactgttttg
       61 ataaataagt aatcgtaaac gatgaaaata ataggctgcg gtatttttat taacgcctac
      121 taactctgct gccgttcttg cagttacacc tgcgacaaac agttcaatga gtttattttg
      181 tttatactgg cttagacgac tttttctcat agggataatt ctaacttaat ttgaatttcc
      241 ctagttatct aggacagccc cttaaaaaat atccctttga aagattttga tgaagtttgt
      301 gaaaagtggg agaaatcctt taaagaaaat ggaaaagaaa ttgaatataa tcaaaaatat
      361 tcaagaattt atggtgaagt tcgtggagct ggctttaatg gcgatatcgg agaaaagcta
      421 catcgttgtg ccgtgatgga atgggctgaa aatgagaccg tgaaattagc ccaaaaatgg
      481 gaacaagagc agaaaaaaca acaaattcaa cagaaaaagg aaactgaaaa atcgccaaaa
      541 cacaaagcca gtcgtgatga ttgggaaatg gaacgttaaa ccgctatttt catgtattct
      601 attaaattat ttgaaaaaag acttcacttt ttgttaaaga aaaatgaagt cttgttctgt
      661 tcaacctaaa ttcagaattt tatctgccga ttgaattgtt tcttggcggt gtgcaaccat
      721 tattttcata ataccaagac tttttaggtt atggttaatt ttttgttcat tttctacatc
      781 taaatggcta cttgcttcgt ctaaaaatag aattttgggt cgtttataca atgcacgagc
      841 caagataact ctctgctttt gtccacctga taagatattt cccatatcgc caatcaaggt
      901 ctcatagccc attggcattt taagtatatc gtcatgtatt tgtgccattt ttgcacattg
      961 ttcaatgagc tccatatttg ggctttcatc aaaaaatgaa atattttccc caatagaacc
     1021 tgcaaaaagg acatcatctt ggcgaacaat cccgctcaat cccctaataa aggatggaga
     1081 aacttgatat atatcatgcc cattaatact aactgtacca gtttcaggtt ttaggctacc
     1141 tgttaaaatg tttaacaaag tggacttccc ccgaccagat tgtcctgtta aaacaactgc
     1201 ttcattatct ttgaattcca aattaatgtt ttcaaaaaga tatggctcat tatcagcata
     1261 tctgaatgag acgttttcaa ctttaagaac cagttgttca ttatctaatt taggtatatg
     1321 attatactta ataatttcag tttctgtttc atttaaagta atgtcagcca aacgttcagc
     1381 atgaagccct aacattttga tttggatgta ttggtcaacg agagaagctg ttctgctttc
     1441 aaattgccct ttataagcca aaaaagccat cagaacaccg actgtaaatg aaccatctaa
     1501 aattgcgctt gcaccaagat aaattatgat aatattttcc atgctaaaca acagtttatt
     1561 tgaaaattca aataaagcag agagtttatc tgttgtcagc ttggtattga ctgtattcac
     1621 aaataggctc atccaagtgc catgtctttg ataatgttta tcaaataatt taactgattg
     1681 gataccacga atggtttcca tgaaatatga gttttgtttg gcttcatgaa caatattttc
     1741 ttctgttgca tttcttaatg ggtaatatgc aagccaacga attagtatgt acaaaacaag
     1801 tgttaaaaga acaatcagcg ataattgagt gctgtaaatt gtcattaaca cgaaagtaaa
     1861 aatagccatt aagctattta aaactaaaac aaaaaaagta gaagttagtg tttcttggat
     1921 atgatctatt gaaccaaatc ttgaaatcac atctcctaaa tgtcgtttac tgaaatagtc
     1981 attaggtaag tcaagtaacc ttttaaatat attggctttc cattgtaaat taagagttgt
     2041 agataggtgc atacctaccc atgcttgtaa caggctaatt aactgttgca ggatagccag
     2101 taaaccaaat cccaaagtaa gggtcagtaa taaattttta tcagcagtta caatgatatg
     2161 gtctattacc cattgcataa agaatggact aaccaatgca aagacttcca aagaaatagc
     2221 taatataagc atttgaatta aagagcgttt taagcctgaa atccccctta atagagataa
     2281 tattttgatt ttctttgttt cttttttctc ttcaaaatgg gtattgggga ataattctag
     2341 ggcaatccct gtgaattttt gtgaaacttc gtccattttg atttttcgca taccgacagc
     2401 agggtccata atgacgatac tgtctttgga aatggaacaa agtacaacaa aatggtttaa
     2461 gttccaatgg agaatgcagg gtagttgtaa atttgacagc tcatctaact ctaaacgcaa
     2521 agctcgtggc gttaaattca tttcattgcc aaatctcatg atgtctgcaa gatttgcgcc
     2581 ctttaatgac agggtgtatt tttggcgtaa cgttcttaaa tcagtgtgaa agccataata
     2641 ggacaagatg gatgtcaggc atgctaaacc acattcagca acttctgttt gcagaatgac
     2701 aggtagcttt ttgttaaatc caaaggacag tcttgataaa taatccattt ttaattgatt
     2761 tttcctgaaa tgctgtaaag tgggtcaagt acccattcgt acaatttttt tcgttcatgg
     2821 agaatatctg cttctaaaat catgccaatt tgaagcggct tgttttctcc gtatgcttta
     2881 atcgtttgtt tttccaattt aactttcaca agataggcag gttcatttaa taaggttggg
     2941 ttagtgaaaa tgatacctaa acctgatagc tcttgtttac cgagagcagt tctggcaact
     3001 gaaataattt ctcctgtggc atgtccaaat ttttggtaag ggtacgcttg gtaacgtaaa
     3061 acaactttat ctttcggttt aataaaacca acagctttac tgggtatgta aagattggcg
     3121 accaattcag tttgttcagg gacaatgctt aacagcaatt tagacggttc aacttgttgc
     3181 cctatatcaa cattaattgt tgaaatataa cctgatttac tagctcgtat ggtttgttcg
     3241 gatttcaaat caaaatccaa aatttcttgg ttcatttccg taatcgcacg gttgagttgg
     3301 ctcaattcgg ttttatggcg ttcaggcagg ctgcttaatg tgattttctg ttcatcaagt
     3361 tccctgattg cattattttg ttcacgtttt aggctctcca aacgtgagcg ttgttccaat
     3421 aaatggcttt cggcggtcat cttatcttgt tgggatactg cgccttgact ggctaaaaac
     3481 ttgttcttgt taagggtttt ttccgctaaa cgaatttgac gattttgccc tgtaatttgc
     3541 tgtttaatat tctctaattg attgtttaaa cgatgaatgt tgttatgaac atttttttgc
     3601 tcattttgat gaatgcgctt taaacgttcc aattcttgta atgccaaagt ttttttaagg
     3661 ttggcttctg ctgccaattt ggcttgtacg tttccttttt cgccaaaacg cgatgtggaa
     3721 agtttgaaca atggttcgcc agctttgaca aagttaccat cttcaacaaa tttatgcgta
     3781 atcgtgccga tatcggaaga gtaaacacga accaccccca tagttggaag taattgacct
     3841 tcaacggttg ttttattggt atagctacca aaaatcaaaa agataatgat acacagagca
     3901 atgagaaaag cgcaaaaagt cagaaataaa aaagagaatg gacgggtcaa gataacctga
     3961 cctgtccact tattttgttg ggctacaaag acttcttttc ggaataagtg ggacatttat
     4021 tattatttct taaaaaaaat ggatagaaaa acaccaataa ttaaatactt atgcctaata
     4081 acataaattt gctattgatt gatgtttcta ttttaattac attaaattta accaataatc
     4141 cagaaatggt tgcagctaaa atgcaaaaga cagtggcgta aatggtttca gtattcacaa
     4201 tttttctctt tcaaaattgt tcaatctaaa tagaaaaagg taacttcaat acatattgct
     4261 gtaactgaaa ttaccttttc cttaatgatg taattatctt atggcttcat catgccacga
     4321 ccgatattgt atccgccagc aacagagaaa ataccagctc ccattgcgtc aacccagcct
     4381 actggtcctg tccacttgtt ccgttgagct acaaagactt ctggtctgaa aaatgagtga
     4441 ttgttagaca tgtgtactta tttatgtttc tttgattggg ataaatttgt aaaaacaaaa
     4501 aaaaggcaag ttgttattat agaagaactt aaaatgctta acatactcat tgaggtttgt
     4561 accacataat gagatacgag ataatttgtt gggagtaaaa tcaaaaaaaa gattaaatat
     4621 ttaaaataat cactatattt tttcattatt tgttcctttt tgtatttaag ttcttatgaa
     4681 aaactagggt attttgaata gaaaatatca aaatacccta gttttattaa ggaatattac
     4741 caccaacaag tgcttccata ttgtacagaa ccagtgattg caccaccaat agctccgaat
     4801 ttcgcacctg gaatagcacc aataccacct gcaaatgaac caacaattgc cccaccagct
     4861 gctccaccta atgcactacc aatggtattt tttgagaaat cacgccagtt acaagcagca
     4921 ccagaaactt gttgaatttc atccaaagtc aaaacttgca taatattcac ctttgttaag
     4981 tgtggttgag aaaaaagtaa attttcatcg ttactcatta tttgcaatca tgcatatact
     5041 gtaaccaagc actatattca gttggatcat agtttttact actgggttgt ctgatatgac
     5101 tattggcact attggcacaa ctgggattag caactttatt gtgagtacct aatggattga
     5161 tatttcctgt aaagtggcta ttgcatgcgc ttaatgagca tagatttatt aatgtcagaa
     5221 aggataaaac aaaaaatctt accatttttt actaaaacca aaagtaggtt tcgaaaactg
     5281 tacaccaaga ccttgtacat atccaaattg cttgctaaca gaaaactcta ctccactatt
     5341 gttaggggaa taggaaacac caattttacc aacagcaacc atatcttttt tacctaggtt
     5401 gccgccaccc tgtgcagaga tatggaattt gccacctgac acttcaacta attcagaggt
     5461 gtgtaactct ttcatgattt tctcctgtaa agaagtatgg ttggaaatgt aaatcagaat
     5521 caatctttct taagtttaat ttaatacttt tttttgcaag tattttattt taaattaaat
     5581 caatatttta aaaatattta cttgtatact gaaaaagaaa ctcctaatgg cggagtgggg
     5641 cttttgttta agtaagcatt gaaaaaattt aaatttccca ttatgattaa cttaatgcaa
     5701 gagctttttc aggacgtaca aaccagcgaa tgatgtgggc aggttggttg tcgctaacca
     5761 atttcagtag ctcaatcgtc agattggtat ccgaccctat caagctaagg agaggctgcg
     5821 gctactacac aaagcacctg aaattattgg gtgcttttgt ctttttatgg cattgtacca
     5881 atttgtgtac cacgagggct tgttcgccca aacgcggtaa caactgaata cacactttcc
     5941 ccctcaactt ctttgcgttc taaaacgact gtcccaatcc gagaatagac aatcatagga
     6001 cgatgattgc cccttacatc actaaattca ctaaaaattt ttgcgcctga ttgaaaaatc
     6061 atttccacat aaaagggtta cacctgtttc atcgtggctt aatccaaacc gcgttaaatc
     6121 tgctttgtgt tctgccaaaa tgtgaatcac accaaagccg tgattaacct ttttgtgttc
     6181 gccgtgtttt aaaacaattt gacctgccgg gaagtctgct ctattgttcg gacaataagg
     6241 ggaatatacc aattacttta actacatcat tttttgacat ttttcttgcc tttcaattta
     6301 aaatccaaaa tcatactgcc ataatttagc aatccaaaaa aatttaggca gcagtaatgg
     6361 tttcgtattt tagaaaacga aacttggttt ggtgttttga agcatttcag tacacaaaca
     6421 tggaatttta agcggtttga gtattcggta ctgtaaccgt ataaacctta ccatttcgct
     6481 tcaaaagacc gaatacggcg actttaccgg cagcaccgcg accgcgtttg cctttgcgtt
     6541 gtccgccaaa ataactttca tctgcttcta cttcgccatc aaacatttcc aaatgcggac
     6601 tgttttgata aataagtaat cgtaaacgat gaaaataata ggctgcggta tttttattaa
     6661 cgcctactaa ctctgctgcc gttcttgcag ttacacctgc gacaaacagt tcaatgagtt
     6721 tattttgttt ataccggctt agacgacttt ttctcatagg gataattcta acttaatttg
     6781 aatttcccta gttatctagg acagccccaa aataaattta gcctaaatca taaaaccgtg
     6841 taattttgca ccaaaaaagg cggtttgcag gtaaaatatc aaaccattaa tttaaatcat
     6901 atttttacag aatattccgc cgcgttcacc aaatggacat caaaccggtt ccaaatttct
     6961 gtaattttgc aacaaaatac cgcaaaacac ccgattgaga ccgaaaggac tttcatatga
     7021 accagacaag ccgcgatctg acccgcatca gccacaacac caaaatcgtc gccacccttg
     7081 ggccgggcag caacaacgtc gaactgttgg aagacatgat ccgcgtcggc ggcctgaacg
     7141 tcgtccgctt caacttcagc cacggcacgc ccgaattcca tcaggaaaac gcccgcatcg
     7201 tgcgcgaggc ggcaaaacgc gccggacagg aaatcgccat cattgccgat ctgcagggcc
     7261 cgaaaatccg cgtgggcaaa atcgccggcg gcggcatcga attgaacaaa ggcgaaacat
     7321 tggtactcga tgccgcgctc gaaggcgaag gcacgcgcga ggcggtcggt ttggactacc
     7381 gcgacctgcc cgacgacgtt gccgcaggcg atgtcttgtg gctggacgac ggcctgctga
     7441 ccctgaccgt ggaatccgtc gaaggcagca ggattatcac aagggtggaa aacagccaca
     7501 tcctgaaaag caacaaaggc atcaacaaac gcggcggcgg tttgtccgca ggcgcgttga
     7561 ccgaaaaaga cttccgcgac ctgaaaaccg cgattgccat cggttgcgac tatctcgcca
     7621 tcagctttgt gaaatccgcc gaagacctgc acatcgcgcg cgccaaagtc gaagaggaaa
     7681 tgaagggcag cactgccgtc cgccccggtt tggtttccaa aatcgaacgc gtagaagcga
     7741 ttgaaaacct tgatgaaatc atccttgccg gcgacggcat tatggttgcg cgcggcgact
     7801 tggcggtcga agtcggacac gccgccgtcc ccgccctgca aaaacggatg atccgccgcg
     7861 cccgcgagtt gcgccgcttc agcattacgg cgacgcaaat gatggaatcg atgattacca
     7921 accccgtgcc gacccgcgcg gaagtcagcg atgtggcaaa cgcggtattg gacggcaccg
     7981 atgcggtgat gtgttccgcc gaaaccgccg tcggcgcgta tccgtttgaa accgtcagcc
     8041 aaatggcgat tatctgcgcg gctgcggaaa aagaacagga ttcgctcaac ggcgttgccg
     8101 aacagacaga gtatcccgaa gccgtcagca ccaacttagc gattgccggc ggcgcggtca
     8161 gcgtggcgcg cgcggttcac gccaaagcca tcgtcgccct gaccgaaagc ggttcgaccg
     8221 cctttgaaat cagccgccac aatattaccc tgccgatttt cgcgctgacc ccgagcgttt
     8281 ccgcccaacg ccgtatggcg atgtaccggg gcgtgcgccc gctgattttg gcaaccagca
     8341 ccgaccacga cacggcgttg aacgaagtcg aaacgatgct ggtggaacac aacatcctgc
     8401 attccggcga ccagtacatc atcaccagcg gctcgcaaat gcgcgaatcc ggttcgacca
     8461 atacgctgga agtgctgcgc gtcaaataat ccgccctgag tggaaaatgc cgtctaaagc
     8521 cgatactgtc gggcttcaga cggcatttta tttgcattcc gtcaggtaaa accgaaagaa
     8581 ttgtgcaaat ccggcaacat cggataaaat cgagtgccta tactaaagcg aaacaaggca
     8641 tttccgactg ccttttttat ttgtccaccg tccgcctttt tacggaaacc gaaatgaccc
     8701 ctttcacact gaaaaaaccg tcctgctgct cggcactgcc tttgccgccg catccgtcca
     8761 cgcatccggc taccacttcg gcacacagtc ggtcaacgcg caaagcacgg caaatgccgc
     8821 cgccgcagaa gccgccgacg catcgaccat cttctacaac cctgccggcc tgaccaaact
     8881 cgacagcagc cagatttccg tcaacgccaa catcgtgctg cccagcattc attatgaggc
     8941 ggattccgcc accgacttta ccgggcttcc cgtccaaggt tcgaaaagcg gcaaaatcac
     9001 caaaaccacg gtcgcgcccc acatctacgg cgcatacaaa gtcaacgaca atctgaccgt
     9061 gggcttgggc gtgtacgtcc ccttcggctc cgccaccgaa tacgaaaaag attccgtgtt
     9121 gcgccacaac atcaacaaac tcggtctgac cagcatcgcc gtcgaacctg tcgccgcgtg
     9181 gaaactcaac gaccgccatt ccttcggcgc aggcatcatc gcccaacata cttccgccga
     9241 gctgcgcaaa tatgccgact gggggattat ggaaaaagcg aaagcactaa aagaaacacc
     9301 ccccaatcca actaaagccg cccaaatcaa agccgacgga cacgccgatg tcaaaggcag
     9361 cgattggggc ttcggctacc aactggcgtg gatgtgggac atcaacgacc gtgcgcgcgt
     9421 gggcgtgaac taccgttcca aagtctcaca cacgctcaaa ggcgatgccg aatgggcggc
     9481 agacggcgcg gcggcgaaag caatgtggaa tggcaatatg ctcgcaccgc tcggctacac
     9541 gccaagcgaa aaagcccgcg ttaaaatcgt tacgcccgag tctttgtccg tacacggtat
     9601 gtacaaagtg tccgacaaag ccgacctgtt cggcgacgta acttggacgc gccacagccg
     9661 cttcgataag gcggaactgg tttttgaaaa agaaaaaacc atcgtcaacg gcaaatccga
     9721 ccgcaccacc atcaccccca actggcgcaa cacctacaaa gtcggcttcg gcggttctta
     9781 tcaaatcagc gaaccgctgc aactgcgcgc cggcatcgct tttgacaaat cgcccgtccg
     9841 caacgccgac taccgcatga acagcctgcc cgacggcaac cgcatctggt tctccgccgg
     9901 tatgaaatac catatcggca aaaaccacgt cgtcgatgcc gcctacaccc acatccacat
     9961 caacgacacc acctaccgca cggcgaaggc aagcggcaac gatgtggaca gcaaaggcgc
    10021 gtcttccgca cgtttcaaaa accacgccga catcatcggc ctgcaataca cctacaaatt
    10081 caaataaacg gcccgccgtt tgattgtaga aatgccgtct gaaacagatt tccgtttcag
    10141 acggcattgt tttaggctcg attgaaaacg aagcaggaaa acaccgccgc catccccgta
    10201 cagacaggaa tgacgggtgc atttttgatt tgttccactg caacaatgcc gtccgaaccc
    10261 gatgttttca ggttcagacg gcatttttat ccggcagccg atatgcctac ctcggtattc
    10321 tattaaacat ttgccaaact gccttcatat caaaacaatc ggaacgctcg ggcagcaaaa
    10381 cggcagcagg cagcttggtg atgccgttca agccgcgtgg gaacagcgcg atccgctctt
    10441 tgcccttttc cgcataatcc gccttgcggt tgtggacgga aaccacttcg cggtggcggt
    10501 aaagcagcgt gttcatttcg atataggctt cgttgtggat ggctgccgac tgtttctgca
    10561 acacgtcaat ctgcctattg agcttgtcgt tggttttcat cagcctgttg aagaacgacg
    10621 gttgctcctg cagtttggcg gtacaggctt cgatgctttc cgataccggc ccgaactgtt
    10681 cgaggtattt ttccaaacag ttttccaagt cggttaggca gcaggccatc ttgcgggaaa
    10741 attcttcttc aggctttccc tgccgaatgg acaatatagt ggattaacaa aaatcaggac
    10801 aaggcggcga gccgcagaca gtacaaatag tacggaaccg attcacttgg tgcttcagca
    10861 ccttagagaa tcgttctctt tgagctaagg cgagccaacg ccgtactggt ttttgttaat
    10921 ccactatatt taggaggggc gtaacccctt ccgaatcagg acggcacata gggcgacgct
    10981 ttatgtgccg tcctgtgtgt tggaacatca gcataggcaa cacaggaaaa gccggctgtt
    11041 ttgcgcctgc gtaccgtatt ggtcaaaatg ccgtctgaaa ccgacaacag gatttcagac
    11101 ggcatttccg tcaattttcc ctttatctgc cgaacaggtt ggcgatattt tccatctgtt
    11161 cttcggtaaa accgttctcg cccaatctgg cggcaactgc ccattcgccc gacaaaccgt
    11221 gttccgcacc gagctgccgc catttttcga ctttggcttc ggctgtggtt ttttcgggca
    11281 gcggtttgac gggaatcctg cttttgggga cggtagtcat cggtttgccc ctaatcgatg
    11341 gaatagccgc gaagatactg tatccgttcg cgcgtcatcc gcgtgtcgca ctgcagcttg
    11401 aggtattcgg cgtattcctg ccggtctgcc tgcgcggcgg cttgtcggca gttgctgatt
    11461 ttttcctgcg cccacttgcg ttgttcgccg accaactctt tttgcacgtc ggtatcgagt
    11521 cctccccaaa gtttggtaat ttcggattcc gcacgctggt tttgtacgcg cgcctcttcc
    11581 acttcgcccc gtgatacggt aacggtatcg gcacgctcgc cgtcgtcggg atgcaggatt
    11641 tccggttcgg gcgcgccttc tgcggcttgg ggtacgtctg catcccctcc ggcggcatta
    11701 tgttccaaaa tgtcttcggg cgtgggtttg gacggttctt cttcacgggc ttttccgctc
    11761 aaaatcctga ccgcgtcttc tttttttacc gccttgccgt ctatcatcac gatgctcttc
    11821 acgccgtaag gcagcaacgc ggcagacagc gtttgcgccg ccataccgac cgtgttgtcg
    11881 acaaatgccg tctgaccgtc tttgacgggt aggaagcgga cggctgccat caatacgccg
    11941 tctttaaact cgacattgcc gcccgtcttc tgccgcacaa tatccgacaa agcggtttcc
    12001 ccgtacagca gggggctgtt tgccttggca tcggcaagcg tttcagacgg cacggtaatg
    12061 ttcaaatcgg cgatacagaa cgtgcgcccg ccttcctgcg tttccgaagc gtgttccaaa
    12121 gaaaacgcca aaccgtaggc ggcggcgata attttgtcgg catcgacaaa ctgcctgccg
    12181 tcttcgcgcg cgaaagaacg cgcttcctgc gtgagcgttt cctgaatatt gccgcgtatg
    12241 ccttgcaaca cggcggggtt ggcgcattcc aatgccttgg gcgtttcttc cctgccgcaa
    12301 gcggcaagca gcagggcaaa cggcagcgca atgagtttcc gatacatgat gttccttcca
    12361 aacggtgcag accgatggcg gctatcatag caaaagcacc tgcaggcggt aaaccttgca
    12421 ggcgctttgt cggaaaggcg gaaaatcagc ctttaaagaa attcacaaaa ccggtgagaa
    12481 tcgcggcatt aatcaaatcg acaaagaacg cgccgaccat aggcacaatc aaaaacgcct
    12541 tatgcgacgc gccgaaagta tgcgtgacgg actgcatatt tgccaccgcc gtcggcgttg
    12601 cgcccaaacc gaaaccgcag tggccggcag ccaatactgc ggcatcatag tcgcgcccca
    12661 taaagacata ggtaacaaaa gtcgcgtaca aaaccatcac tgcggtttgt actgcaagaa
    12721 tcacggttac agaccccgcc aaaccggtca gctcccacag tttcaaattc agcaacgcca
    12781 ttgccaagaa aagcgaaagc gaagcattgc cgaacacatc gatggcacgg tcgaacatat
    12841 tgaccttgaa tgcggcggta aggatgttgc ggataaccac gccgccaaac agacaccaca
    12901 cgaatttggg caggtcgaac aggtattctt tgtcgaagcc gtccataatc tcggcaaacg
    12961 ccagacacgc ggcaaacatg gcaagcgttt caacggcaga ttccgccgta atcaggcggg
    13021 tgcgttttgc ctgctcgaac acgtcgtccg cgttgtcgtc ctgatcctgt tttgtgtttt
    13081 caaccggttt gcggcccatt ttgttgatca ggcggcgcgc aaccggcccg ccgatcaggc
    13141 cgccgaacac cagcccgaaa gtagccgaag caatacccaa accggttgcg ccgaccaagc
    13201 cgtattgcgt ttcaaaatta ggtccccacg cacctgacgt gccgtgtccg cccgtcagcg
    13261 acaccgaacc ggtaatcaga ccgatgagcg ggtccaaacc caaagccgta gccagtccga
    13321 ccccgacaaa gttttgcacc aagataaatc cgcccacaat cgcggtaaaa accaccagcg
    13381 gcaaaccgcc cgccttcaaa cgggaaaaat ccgcgctcaa gccgatggac gtgaagaaaa
    13441 tcagcataaa cgcattttgc agcggtttct caaatttgaa gctcacgccg tacgcctcgt
    13501 gcagcgcaaa caggataatc gcggcaatca gcccgcccgc cacgggttcg gggatgttaa
    13561 aatcacgcaa gattttgatt ttcttaacca aaaccttgcc gaccaacaaa accaaagtgg
    13621 cggcaatcaa ggtgtaataa ctgttaaacg cccattccat atcgtttgac ctcctaaaat
    13681 gtttttcttt atatccgccc gaaggggacg caagcgcgaa gatattaggg gaatagttaa
    13741 ggcttgtcaa agaatcgccg ttttttatag tggattaaat ttaaaccagt acatcgttga
    13801 ctcgccttgc cgtactatct gtactgtctg tggcttcgtc gccttgtcct gatttaaatt
    13861 taatccacta tacaactatt ttttacaaac aaaatgccgt ccgaaactcc gttcagacgg
    13921 catcatggga atacacatat ttgaaataac agaaaaagcg ggggcaaaac ccgccgctgc
    13981 atctgtcgct gtacatccct gctgtcggac ttaccgcttc aacttcgcaa acgcatccgc
    14041 catcgccgaa ttggttggga cacgctcgtt gcgttgtggt ttgcggccgg ctgtccgttc
    14101 gcggctgcgg tttttccagc tcggacagaa tgtccagaat ggtcggcagt ccgaagtgtt
    14161 cgtcggtgaa gtcggacgct ttgatttgct tcacgcgctc gcggttaact gcctaaatat
    14221 gaaattcttg gtcttcttgt agcataagtt ctcgatgata tgccaagtaa gatttacgtt
    14281 tgtcgttgag cagattgtta tctagcttca catcacacca acgtcgccaa acatcatctg
    14341 ataaccgctt agatttcacc atatttcctt catcatcaaa cgaaatatat ttcaaatcaa
    14401 ataggctatc caaggtacga cttaatagca agccgttatt tgggtcgtat gcttcggtat
    14461 catccgacag gatgaaaggc ttaatatgac tggcaatcaa tacaggatag gcaagttttt
    14521 ccagcataca tttcacatta ccgtaatgtt cttcactttc ttcttgaagt tggtttttgt
    14581 ataggcgatg taaataaggg tcgcgtttcc ttacagtaat ttcatccaaa ttaccaaaaa
    14641 tacgttgggc atcctcagca aaatataagt catcacctac tcgttttaag tcgtctaatt
    14701 tgtctaatag gttccataaa taacttattt gattgtattt tcgttcaata aatcctgatt
    14761 ctattccctg ttgaaaataa tcattcaatt ctgtttcggt tagataatct tgttgaaagg
    14821 tttttaagtc tactaacatc atggcggcaa tttctttttt atttagcttg ccttgtggat
    14881 gctcgaccag cgttttaatc aggaaattta tttgtcgaat attagagttt tcagttactg
    14941 aacgctgaaa gccagagtgc gtatatacga ttttggaaag tagggtttgt cgttttcggt
    15001 tagtacgagc ttgtacataa tctcgtgata aaggtgtata gcttgataaa aacggttcta
    15061 taaatcccat ttttacaaat tgattaatag ctttacgaac cgaggccaaa ttcatggcct
    15121 tgcttgttct ctgcgaagcc ataacggaat cttgggcaat gttttgctgc aatgcttcat
    15181 atagttcgtt gctgtatggc tggtcttgat tcgcatcaat aaagtcaaga caaatattga
    15241 gtgcagtaca aaatttttga tcgttataat ttgtaaaggc attggtaaac gcccaataat
    15301 cttcataaat ttggctgttt ggctgattat tactcatctc tgtctccaag aatattcgat
    15361 accaatattc tgtttttgta tttaagtaat atctatcatt catgtatttt ctactaaaaa
    15421 cagaatttcc ttatggcttt gcttcttttc ttttcctgta accttataaa cgtgaggcgt
    15481 ttccaagatt tgtacatttc gcccattttt acctaacatt tcttctaatt catctcggtt
    15541 tggatatgaa gcattgttgt agcttaacag ccaatattta aatggtttca aatgattgat
    15601 taccctttta aataattcga cagcctgctt tttatccatg aaatgattgg caaatggttt
    15661 gggtatggat gatgtaatgt aactgtccaa taatccgtaa aagccaaaat aattgttcat
    15721 cgtgcctgtg taaggtgggt ctaaatagac ggcatcagct tgtatattag gtaataaatc
    15781 aaatatatct tgattaaagg cgtgatgccg ccagccatta ttgaaaacag cttggttata
    15841 gtcatccaaa ttttgcagaa aatgactttg aaaactttca ttgtggtagg cgcgtcgacg
    15901 gccatattta gcataactgt attcttcatc acgcagttgt ttaacttttt cccaaggaat
    15961 tgtgaatcgt gaatagggca ttttacgtat catggcgcga cgcattaaag taaacgccaa
    16021 agcacgttta tactggttat tcagcttccc tatattttta cggtacaaat caagttgttg
    16081 gcattcatcg tgaaaataga atttttcgga ataacgctga ctcataaaac cggcatgcgg
    16141 gctgcctgaa aaaatcattg cgacatcatc gtcatttaat gtttcatggt tgttttctat
    16201 taatgctaaa gcaatttggt aattaattgc caaaatatcg ttagtaatca cacgatagcc
    16261 gcgttttttg gcttcgtagg caaaggaaca gcctccacta aatacatctg caactgtatc
    16321 tacatcagac ggaagctggt cacaaatcca ggaagctatt ttctctttat taccgatata
    16381 gttaattttc ggatatttct tattcatttt cactcatttt taaaatactt tcagcaatag
    16441 cttttgccaa taaaggcggc acggcgttac caacctgctg ctgctgggca attttgctgc
    16501 cgcaaaaaat aaaattatca gggaaagatt gtaaggcagc taattcacga acggttaaag
    16561 cccgattctg ttcatagtga aaaactttgc gcatatctcc tgtaatacaa acggctggtt
    16621 tgttgctgtt gtaacggatg tatttacgga tatcacctgt tttcggacgt aatggttcag
    16681 gaatatcgtt acggttacct ccatttttaa caaatgccat tttttctaac atttgtgccg
    16741 aatgattcat agcttcatga tttgcaacgt gtggattgct ttcgccagca gccagttttg
    16801 gaaaatgtcc tattgctgat ccaacagtct gatgggaaat ctgcaaaggt tcgggaaagg
    16861 aaattttgcc tttatccctc ctcccgataa atatcactcg gctacgtatc tgaggaacac
    16921 cgaaatcggc tgcactcagt atcttacatt ccaccgaata accgatattc tgaaatgctt
    16981 gaataatctc aatacgtgtt ttacctgaat tgtgtgtata gagtcgcgct acattttcca
    17041 taacaaaaaa atatggttgg acaattttaa ctattcggac aaactcttta aataaatggt
    17101 tgcgtgggtc atctgtaaat gtccgtccaa tctttcctgc catactaaaa ccttgacaag
    17161 gtggtcctcc aataaccaaa tcaactgatt gtccgttaag acaattggtt aaatcttgtt
    17221 cggttagtgt ggttaaatct ttttgcagta attgatgacg ggggaagttg gtacggtaag
    17281 tctgacaata atcagactcc atttcaacag aaagcaattg ttggaatccg gcttgttcaa
    17341 aacccaagga taggcctcct gctcctgaga aaaggtcaat ataggtaagt ggtgtgtgca
    17401 taagtcaaaa tccataaact cttcgattat tttaccgctt caacttcgca aacgcatccg
    17461 ccatcgccga attggtcggg gcgcggtcgt tgcgttgggg tttgcggccg gctgtccgtt
    17521 cgcggctgcg gttttcagac ggcattttat gttttgcgcc gcccggttcg tcatccaagc
    17581 gcatggtcag cgcgatgcgt ttgcgtgcgg catcgacttc cagcactttc acttttacca
    17641 cgtcgccggc tttcaccacg tcgcgcgggt cttggacgaa cttattagac agggcggaga
    17701 tgtgcaccaa gccgtcctga tggacgccga tgtccacgaa cgcgccgaag ttggcgacgt
    17761 tggagaccac gccctcgagt atcatgccga cttgcaagtc gctgatttcg tggatgcctt
    17821 cggcaaacga tgccgtctga aacgcaccgc gcgggtcgcg gccgggtttt tccagctcgg
    17881 acaggatgtc caaaatagtc ggcagaccga agcgttcgtc ggtgaagtcg gatgctttga
    17941 tttgcttcac acgctcgcgg ttgccgatga gttcggcggc ggtaatgcct tgttgcgcca
    18001 gcattttggc gacgacggga taggcttcgg ggtggacggc gctcgcgtcc agcggctctt
    18061 tgccgccgtt aatccgcaaa aagcctgctg cttgctcgaa ggttttttcg cccaagcgcg
    18121 gtacttttag caattttttt cggctgtcga acgcgccgtt ttcatcacgg taggcgacga
    18181 tgttttgggc aagggtttga ttcaagccgg aaatccgcgc caagagcggg gcggaggcgg
    18241 tgttcgcgtc cacgccgacg gcgttcacgc agtcttcgac caccgcgtcc agcgatttgg
    18301 cgagccggct ttggttcacg tcgtgctgat actgccccac gccgatggat ttggggtcga
    18361 ttttgaccaa ctcggcaagc gggtcttgca gcctgcgggc gatggacact gcgccgcgca
    18421 gggaaacatc taagtcggga aactcgcgcg ccgccagttc ggacgcagaa taaatcgacg
    18481 cgccggcttc ggaaacgacg attttgtgca ggctgctttc gggcattccg cgcaccagtt
    18541 cgcccgcgat tttgtcggtt tcgcggctgg cggtgccgtt gccgatggcg atgagcttca
    18601 caccgtgttg tttaatcagg cgcgacaacg ttgccaacat attgttttct tgatgcaaat
    18661 agacaatgac cgtatccagc agtttgcccg tgtcgtccac cacggcgcat ttcacgccgt
    18721 tgcggtagcc ggggtcgagt cccaaagtgg tcagccgccc ggcgggcgcg gcgagcagca
    18781 agtctttgag attgcgggcg aacacggtaa tcgcgtcagt gtcggcagct tctttcaaac
    18841 gattgagagc ttcgagttcc agcgacaaaa agattttcgc gcgccaagtc aggcgcacgg
    18901 tgtcgcgcag ccatttgtgg ccgtctgaaa ccttgaagcg gcgggcgatg atttgctcgt
    18961 attcgctttg ctgcgtaatc ggcgtgtcgt cgggctggta tttgagcgcg atgttcaaca
    19021 cgccttcgtt gcgaccgcgc aaaaccgcca gcgcgcggtg gctgggcatg gcgcgcacgg
    19081 gttcgcggcg gtcgaaataa tcgctgaatt tttcgccttc ggtttctttg ccttcaacga
    19141 cttgcgtgtg gatttcggct tcgttccaca gcttgtcgcg cagcgtgccg atgagttccg
    19201 cgtcttcggc aaactgctcc atcagaatcg cgcgcgcgcc atccagcgcg gctttggcgt
    19261 cggggatgtt ttcgttcagg tagccttgcg cggcggcttc cacgtcctgc ggctgttcgg
    19321 caagcaacac gtccgccagc ggctgcaaac cgtgttcgcg agcgatttgc gctttggtgc
    19381 ggcgtttggg tttgtagggc aggtacaggt cttccaacgc ggttttgttg tcggcggctt
    19441 cgatttgcgc cctgaggtcg tctgaaagct tgccttgctc ttcaatgctt tttaacacaa
    19501 cggccttgcg ctcttccaac tcgcgcaggt attgcagccg ctcggcaagc tggcgcagct
    19561 gcgtatcgtc cagcccgccc gtggcttctt tacggtaacg cgcgataaag gggacggtcg
    19621 cgccgtcgtc caaaagctcg atagcggcat tgatttgcac ggtagtggcg gagagttctt
    19681 gggagaggat tcgggtgatg ttcataaatt gcctccgtga tgccgtctga acagccgacg
    19741 gcgcaatgtg ggtaatcgtt tggaaaagat gaagataata ctgatatact ttgcccgata
    19801 caatctgaaa ggaatgttta tgaccgtcct gattaccggc ggcaccggct ttatcggttc
    19861 gcacaccgcc gtctcgctcg tccaatccgg ttacgatgcc gtgattttgg ataatctgtg
    19921 caactcgtct gccgccgtcc tcccgcgcct tcggcaaatt accggcagaa acataccgtt
    19981 ttatcagggc gacatccgcg actgtcagat tttgaggcag attttttcag aacatgaaat
    20041 cgaatccgtc atccattttg ccggtttgaa ggcagtgggg gaaagcgttg ccgagccgac
    20101 aaaatattac ggcaacaatg tttacggcag cctggtgctg gcggaagaaa tggcgcgcgc
    20161 gggcgtgttg aaaatcgtat tcagctcgtc ggcaaccgtt tacggcgatg cggaaaaagt
    20221 cccctatacg gaagatatgc gcccgggcga taccgctaat ccttacggtg cgtccaaagc
    20281 gatggtggag cggatgttaa ccgacatcca aaaagccgat ccgcgttgga gcgtgatttt
    20341 gttgcgctat ttcaacccga tcggcgcgca cgaaagcgga cttatcggcg aacagcccaa
    20401 cggcgttccc aacaatcttt tgccctatat ctgtcaagtg gcttcgggca ggctgccgca
    20461 actgtcggta ttcggcggcg actatccgac ccccgacggt acgggaatgc gcgactacat
    20521 ccatgtgatg gatttggcag aagggcatat cgcggcaatg aaggcgaaag gcggcgttgc
    20581 cggcgtacat ttgttcaact tgggttcggg acgcgcctat tccgttttgg aaatcatccg
    20641 cgcctttgag gccgcatccg gtttgcacat tccttaccga atccaacccc gccgcgccgg
    20701 cgacttggcg tgttcctatg ccgacccgtc ccataccaaa caacaaaccg gctgggaaac
    20761 caaacgcggc ttgcagcaaa tgatggaaga ttcgtggcgt tgggtcagcc gcaaccccgg
    20821 cagatatggg gattaaatgc aaaccgcagg caaaaaaaac atcctcgtta ccggcggcgc
    20881 gggttttatc ggctcggcag tcgtccgcca tattatccaa aacacccgag attccgtcgt
    20941 caacctcgac aagttaacct acgcgggcaa tctcgaatcg ctgaccgaca ttgccgataa
    21001 tccccgctac gcttttgagc aagtggatat ttgcgaccgc gccgaactcg accgcgtgtt
    21061 cgcgcaatac cggcccgatg ccgtgatgca cttggcggcg gaaagccacg tcgaccgcgc
    21121 catcggttcg gcaggcgaat ttatccgaac caatatcgtc ggcacattcg acctgctgga
    21181 agctgcgcgc gcctattggc agcaaatgcc gtctgaaaaa cgcgaagcct tccgtttcca
    21241 ccatatttct accgatgaag tctatggcga tttgcacggc acagacgatt tgtttacaga
    21301 aaccacgcca tacgcgccgt ccagccccta ctccgcctcc aaagccgccg ccgaccacct
    21361 tgtccgcgcg tggcagcgga cttacaggct gccctccatt gtcagcaact gttccaataa
    21421 ttacggcccc cgacaattcc ccgaaaaact cattcctttg atgattctga acgcactttc
    21481 cggcaaacca ctgcctgtgt acggcgacgg ggcgcaaatc cgcgactggc tgtttgtcga
    21541 agaccacgcg cgcgcgctgt atcaggtcgt taccgaaggt gttgtcggcg aaacctacaa
    21601 tatcggcgga cacaatgaaa aaaccaatct cgaagtgatc aaaaccatct gcgccctctt
    21661 ggaagaactc gcccccgaaa aaccggccgg cgtggcacgg tatgaagatt taattacttt
    21721 cgtacaagac cgccccggcc atgatgcccg ctacgccgtc gatgcggcga aaatcaggcg
    21781 ggatttgggc tggctgcctt tggaaacttt tgaatccggc ctccgcaaaa ccgtgcaatg
    21841 gtatttggac aacaaaaccc ggcggcaaaa cgcataaaac ggcaactgcc gttcggaacg
    21901 cttaggtacg ggcaaatagt tttcagacgg catcccgacg caatgccgtc tgaaaaccca
    21961 tcgcaaagga agaaggaaaa gatgaaaggc atcatactgg caggcggcag cggtacgcgc
    22021 ctatatccga ttacgcgcgg cgtatccaaa caactgctgc ccgtgtacga caaaccgatg
    22081 atttattacc ccttgtcggt tttgatgctg gcgggaatcc gcgatatttt ggtaattacc
    22141 gcacccgaag acaacgctgc cttccaacgg ctactcggcg acggctccga cttcggcatc
    22201 cgcctgcaat atgccgttca acccagcccc gacggcttgg cgcaggcatt tatcatcggc
    22261 gaagaattta tcggcaacgg caatgtctgc ctgattttgg gcgacaatat tttctacggt
    22321 caatcattta cgcaaacatt gaaacaggcc gccgccaaga cccacggcgc aaccgtattc
    22381 ggttatcggg ttaaagaccc cgagcgtttc ggcgtggttg aatttgacga aaacttcaac
    22441 gccttatcca tcgaagagaa accgcaacag cccaaatccg attgggcggt aaccggcttg
    22501 tacttccacg acaaccgcgc cgtcgagttc gccaaacagc tcaaaccgtc cgcacgcggc
    22561 gaattggaaa tttccgacct caaccggatg tatctggaag acggctcgct gtccgttcaa
    22621 atattgggac gcggtttcgc gtggttggac accggcacgc aagaaagcct gcacgaggcg
    22681 gcttccttcg tccaaaccgt gcaaaatatc caaaacctgc acatcgcctg cctcgaagaa
    22741 atcgcctggc gtaacggctg gctgacgaaa aaagatgtgg aaacacgggc aaagcctttg
    22801 gaaaaaaccg cctacgggca atacctgctg cgcctgatcg gcaaataaat accgtctgaa
    22861 cgccccgacg gctttcagac ggcatccaaa ccgtgccgcg ccggcacctt aaccgacctg
    22921 ccgcacggcg cgcataaaaa atcaaaccgc aaaaaaacag cggtaacgat accgccaaag
    22981 gactttatgg acatcatcga caccgccctc cccgacgtaa aacttttaaa accgcaagtc
    23041 ttcacagacg ggcgcggttt ttttatggaa acattccgcg acggatggtt caaagaaaat
    23101 attgccgatc gaactttcgt gcaggaaaac cactccaact ccagcaaagg cgtattgcgc
    23161 ggcctgcact accaaaccga aaacacccaa ggcaaactcg tacgcgtagt tgtcggcgaa
    23221 gtgttcgacg tggccgtcga tatgcgcgaa ggttcgccga ctttcggcaa atgggcgggc
    23281 gcaaccttgt cggcgcaaaa ccgataccag ctttggatac ccgaaggctt cgcacacggt
    23341 ttttgcgttt tgggcgacgc ggcggaagtc gtgtacaaat gcacggacta ttacaaccct
    23401 gaaaccgaac aggttttaat atggaacgac ccggcaatcg gcataggctg gccgcttcaa
    23461 accgcgccgc tgctttcgcc caaagacctt gccggcaaaa cgtgggcaca agccgaaaag
    23521 ctccgcctta cgctttcccg ataaaaaatg ccgtccgaac gtttcagacg gcattttctc
    23581 cgacaggcta cctacccgcc ttcagtacgc gctgtgcaaa gaaaaacatc ccggtaacga
    23641 agaacgccaa gcccaaccaa gaagcggcgg catcccagtt ttgcagattc aacgcgaacg
    23701 gcgcatccga gccgccgttg gtcacagaga aggcaataat aaacgccata attacaccaa
    23761 tcaggctttc accgacaatc aggcctgccg cgaaaagcgt accgatacgg tcggcgtttt
    23821 tcagacggcc ttcgcggttt tccgccttct tgccaatgat gtgtttcaac accgccgcca
    23881 acaccgcgcc tgccacgatg ggcatattga cggacggcgg cagataaatg cccataccga
    23941 ccgcaaggac gggcagggca agtttaccgc ctgatgattt tttcaacacc aaatcgacga
    24001 cgattaatac cgctccaatc gcgataccgg taaagatgta cgcccattcg aggttgtggg
    24061 cgaaaatgcc cgacgcaatg gtcgtcatca aagtcgcttg aggggctgcc aaagcctgcg
    24121 ccgcatccat gccttcgcgc ggcattgcgc cggtaaagcc gtaggcttcg taaagcagtt
    24181 ccaacacggg cgaaataacc agcgcaccaa cgatacaacc gataatcagg gcgacttgct
    24241 gccgccaagg cgtagctttg agcaggtagc cggttttcaa gtcttgcagg ttgtcgttgg
    24301 aaatcgaagc cacgcagatt actgccgatc cgcaaaaaag cgtcaacgcc agtaagaatt
    24361 tgcggttggc ctcatccgcc aacaaacctc cggattcgcc taccagcagc aaaaccagtg
    24421 aaataacgac gatggacacg atgcccacgc cggaaatcgg gctggaagac gagccgacca
    24481 aacctgccat ataaccgcag gcggcggcga ccaaaaagcc gatgacggaa gccaaaagcg
    24541 tgcaaaccgc caccaaaagc cacgccatgc cgcccgtaat gtgcgaatcg ccgataaagt
    24601 ggtaaaacga cacgcctaga ataaacatca tagacaacac ccagaaaatc atggctttag
    24661 gcgacaaatc ctgttcggcg cgttctgtag cgggcgcacc gccgccaaaa ctcttgaacg
    24721 acatcttcat gccttccacc attggcttga gcagcatcaa aagcgtccaa accgccgcga
    24781 tacctatggt tcccgcaccg ataaaacgca ctttttcctt ccacagcttc atcgcaaacg
    24841 ccgccatttc catatcggaa ggttgcggaa tgtgtgagga gaaatacggc acggcaatac
    24901 cccaagcaat cgaaatgccc agcaggatgg cgatgccgcc cgtcagtccg accaaataac
    24961 ccgcgcccaa caaggccagt gaaaagccca tcggcagttg gaaaattgcc gtaccgcttt
    25021 taaaccaata actcgcgcta tcggcaatca cacgcagacc tccggcgcaa aagctcatca
    25081 atcccgccaa cgcgccgccg gccgccagct ctttgatacc gctgccgccc tgacggtcat
    25141 ccccttcttc atgaccgccc actttcaaaa tttcagcagc cgccacacct tccggataag
    25201 gcaaatcgct tttcaccacc atcgcgtaac gcagcggaat ggtgaaaatc acccccaaaa
    25261 tcccgccggc aatacataaa agcgtcgtct gccaaaacgg gaccgctcca gtagcccgcc
    25321 atcagcaaac cgggcaggac gaagatgatg gttgaaagcg tacccgcagc cgaggcttgg
    25381 gtctgcacca tgttgttttc caaaatattg ctgcctttga aaaattttaa aaccgccatc
    25441 gaaatcaccg ccgccggaat cgacgaggca aaggtcagcc cgactttcaa accgaggtaa
    25501 acgttagacg cagtaaaaat tacagtgatc aatgcgccga gtatcatgcc tcggagcgtc
    25561 agttcgcggt attcttctac cgaaccggat aaagatttat tcattattct tcctttgaca
    25621 acagacgttc acatattgtt ggcatcacgc catgatgtca agttttaaaa agaacagtta
    25681 aaaacaatta tcccaccctg cccataccca attgaaaata aaaactattt ccaaacgaac
    25741 aaaaacagcc gtatcaggga ggttccccga tacggctgct tgtttccgaa ccttaaaatc
    25801 aatcaaacaa atcgcgcagc ttgtctaaaa acgatttctt gcgcggcgtt tggttttcca
    25861 agcccgtaga aatccgctca aattcttcca aaagctcttt ttgacggtcg gtcaaattga
    25921 caggcgtttc gacaacaata tggcagtaca aatcgccggt cgcgctgctg cgtaaagatt
    25981 tgacaccctt acctttcacg cgcatcctcc tgccggtttg ggtttctttg gggacggtga
    26041 gcttgacttt gccgtccaag gtcggcactt ctaactcccc gcccaaagca gccgtggcaa
    26101 aactaatcgg cagttcgcaa tgcaaatcca aaccatcgcg ttggaaaatc ttatgcgccc
    26161 gaatgcggac ggttacatac aggtcgccgg caggcgcgcc gtgcataccc ggcccgcctt
    26221 caccgctcaa acggatacgc tgcccgtcat cgataccggc gggaatattg acttccaccg
    26281 tcttgaccgc cttattccgc cccacgccac ggcatttgac gcaaggttct ttaatatgtt
    26341 tgcccgcacc gcggcaggtc ggacaagtct gctgcatacg gaaaatcgcc tgctggatgt
    26401 gcaccgtacc cgaacctttg caagtcgggc aggtttccgg ggatgccccc ggtttcgcgc
    26461 cgctgccgtt gcagacatca cacgcttcat aagtcggaat attgatgcgt ttcttcacac
    26521 cttttgcggc ttcttcaagc gtgatttcga taccgacctg cacgtcctca ccctgataat
    26581 caggctgggc gcgccccgaa ccacctccaa acatttggct gaaaatatcc ccaaagtcaa
    26641 aaccctgcgc accgccaaat ccgccaaacc ctccgaagcc cccctgtccg ccgccttcaa
    26701 acgccgcatg accatactgg tcgtacatgg cacgcttttc cttgtcggac aaagtttcat
    26761 acgccttttg tacttcttta aacttctctt ccgcctcttt attgtcagga ttgcggtcgg
    26821 gatggtattt catcgccaat ttacggtagg cttttttaat ctcatcatcg gtagctgctc
    26881 ttgccacacc cagcgtcgca taaaaatctt gattactcat tttttcatct aaaaataaaa
    26941 tcacggctta aaatgagggc aattgcgcaa aacacaagac aaacagactg agacctttgc
    27001 aaaaatcccc taaaatcccc taaattccca ccaaggcatt taggggattt cccatgagca
    27061 ccttcttccg gcaaaccgcc caagccatga ctgccaaaca catcggccgc ttcccgctat
    27121 tggagttgga ccaggtgatt gattggcagc cgatcgaaca atacctgaac cgtcaaaaaa
    27181 cccgttacct cagagaccgc cgcggccg
//
LOCUS       DBJGGI010000023        25656 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.023, whole genome shotgun sequence.
ACCESSION   DBJGGI010000023 DBJGGI010000000
VERSION     DBJGGI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25656
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.023"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(116..835)
                     /gene="pyrH"
                     /locus_tag="ACSIGJ_000968"
     CDS             complement(116..835)
                     /gene="pyrH"
                     /locus_tag="ACSIGJ_000968"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HHQ1329857.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEIVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALNKNLKVMDATAFALCRERKLNIVVFGI
                     AKQGSLKRVITGEDEGTLVHC"
     gene            complement(1052..1906)
                     /gene="tsf"
                     /locus_tag="ACSIGJ_000969"
     CDS             complement(1052..1906)
                     /gene="tsf"
                     /locus_tag="ACSIGJ_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HHQ1329858.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVESERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENDTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(2035..2763)
                     /gene="rpsB"
                     /locus_tag="ACSIGJ_000970"
     CDS             complement(2035..2763)
                     /gene="rpsB"
                     /locus_tag="ACSIGJ_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HHQ1329859.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQEAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDHRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQETAAE"
     gene            complement(2936..3169)
                     /locus_tag="ACSIGJ_000971"
     CDS             complement(2936..3169)
                     /locus_tag="ACSIGJ_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329860.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWVVLPTVFTVFA
                     VTVRRRNRLMGQQEQAESEQQRAQRQKDSGTKP"
     gene            3247..3828
                     /locus_tag="ACSIGJ_000972"
     CDS             3247..3828
                     /locus_tag="ACSIGJ_000972"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HHQ1329861.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAAAVKINRLLKRYIKRGR
                     KIGVYWPMGKELRLGGFVRAAQKRGAKLYLPYIEPHTRRMWFTPYPERGMERERKRGR
                     AKLHVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLSAMKYRLQAKTV
                     GVGFACQLVDRLPREAHDLPLDGFVSEAGILCF"
     gene            3889..4479
                     /locus_tag="ACSIGJ_000973"
     CDS             3889..4479
                     /locus_tag="ACSIGJ_000973"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHQ1329862.1"
                     /translation="MSERIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEADRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRKRINTCCKIMLLDYAFDVLACCCAGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
     gene            4863..6329
                     /locus_tag="ACSIGJ_000974"
     CDS             4863..6329
                     /locus_tag="ACSIGJ_000974"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HHQ1329863.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
     gene            complement(6516..6890)
                     /locus_tag="ACSIGJ_000975"
     CDS             complement(6516..6890)
                     /locus_tag="ACSIGJ_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7606 domain-containing protein"
                     /protein_id="HHQ1329864.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPINLDKSDNMDTFYGKEGGYVLSTGAMDSKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(7128..7427)
                     /locus_tag="ACSIGJ_000976"
     CDS             complement(7128..7427)
                     /locus_tag="ACSIGJ_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHQ1329865.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            complement(7526..8305)
                     /gene="map"
                     /locus_tag="ACSIGJ_000977"
     CDS             complement(7526..8305)
                     /gene="map"
                     /locus_tag="ACSIGJ_000977"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HHQ1329866.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGITTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGGVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPATGKP"
     gene            complement(8345..8995)
                     /locus_tag="ACSIGJ_000978"
     CDS             complement(8345..8995)
                     /locus_tag="ACSIGJ_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329867.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRPTPKLLIRAVLLICI
                     TAIGALAVGIVSTFNPNGDKTLQTEPQHTDSPRETEFWLPNGAVGQDAAQPEHHHAAS
                     SEPAQPDGTEESGSGLPSPAAPKKNRVKPRPSDAARAADSLTGTGTQAENTLKETPVL
                     PTNAPHPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPAKPHKEILDNLF"
     gene            complement(9003..9611)
                     /locus_tag="ACSIGJ_000979"
     CDS             complement(9003..9611)
                     /locus_tag="ACSIGJ_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HHQ1329868.1"
                     /translation="MKPKPHTVRTLIAAVLSLALGGCFSAVVGGAAVGAKSVIDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIIT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            complement(9675..10268)
                     /locus_tag="ACSIGJ_000980"
     CDS             complement(9675..10268)
                     /locus_tag="ACSIGJ_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HHQ1329869.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAEKILVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAMLKDT
                     DVLLNVPHPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            complement(10273..10620)
                     /locus_tag="ACSIGJ_000981"
     CDS             complement(10273..10620)
                     /locus_tag="ACSIGJ_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HHQ1329870.1"
                     /translation="MRLNHKQGTAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNQRFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GNRPPEWIKNITG"
     gene            10667..11542
                     /gene="rsmI"
                     /locus_tag="ACSIGJ_000982"
     CDS             10667..11542
                     /gene="rsmI"
                     /locus_tag="ACSIGJ_000982"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HHQ1329871.1"
                     /translation="MFQKHLQKAPDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGRLVSVREHNERQMADKVIGFLSDGLVVAQVSDAGTPAV
                     CDPGAKLARRVREAGFKVVPVVGASAVMAALSVAGVAESDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPVVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALAADGNQSRGEMVLVLYPAQDEKHEGLSESAQNAMKILAAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            complement(11577..12107)
                     /locus_tag="ACSIGJ_000983"
     CDS             complement(11577..12107)
                     /locus_tag="ACSIGJ_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HHQ1329872.1"
                     /translation="MNTNQPAVYDPLTRALHWLTVAGFIGILTTIVLWTIYEEAEWAG
                     SLFGLHKSFGFLMLTVIALRIVWAVANRAKRPQSDSKAAAAGHGILYLLMLAVPVIGM
                     IRQYGGGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGWLLFAAVVGHVAMVVVH
                     RVQGKDVLYRMTGRVR"
     gene            12386..13540
                     /gene="obgE"
                     /locus_tag="ACSIGJ_000984"
     CDS             12386..13540
                     /gene="obgE"
                     /locus_tag="ACSIGJ_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HHQ1329873.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEARARTAAFLEAVGWDYPEPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLAEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            13594..14541
                     /locus_tag="ACSIGJ_000985"
     CDS             13594..14541
                     /locus_tag="ACSIGJ_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HHQ1329874.1"
                     /translation="MYKTIDLFSGIGGIRLGFEKYGCTNVFSSEWDKYARQVYEANFG
                     EKPFGDINGIDPSDIPDHDILLAGFPCQPFSIAGKGLGFEDTRGTLFFNIAEILKTKQ
                     PKAFLLENVKRLTTHDSGRTFRIVLETLKQLGYTVYFKVLNTLDFGLPQKRERIYIVG
                     FSDNIPFYFPEPINQYRPLGELLENDRDVEPSYFLSDTLKQKRLAALKKAPPTPSIWH
                     ENIGGNVSALPYSCALRAGGSYNYLVVNGVRRLTGREMLRLQGFPDDFEINIPYSQVR
                     KVAGNSVSVPVIEAIAENMLASLSGKVEQKGQLDLLEAG"
     gene            14538..15590
                     /locus_tag="ACSIGJ_000986"
     CDS             14538..15590
                     /locus_tag="ACSIGJ_000986"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HaeII family restriction endonuclease"
                     /protein_id="HHQ1329875.1"
                     /translation="MTLEEQQAKEALDGIIKKSRVHLYKPIQIAEILYHDRCIKQLDF
                     LNLDTYRNQSKRWRDEICRRFLGRISTSSAKFQDNLFEKNAIPPEKLAVLGTLNRQSD
                     GGVESYIYKQFFNRFSQMSEALAYVGNTDRYSFQLSEFLNLFWLEPGLKRSIDKIYEI
                     VVYALFDALVSELGITVSIDFPKENLFLWEEYQDFAEKIITMPKNEHLKLPAKIHRVG
                     VTNAADRGLDMWSNFGLAIQVKHLSLDEELAEDIVSSISADRIVIVCKKAEQSVIVSL
                     LTQIGWKSRIQNIVTEDDLISWYEKALRGQYPIAEALLENIKTEIMREFPAVNEANEF
                     LDFAQNRGYDITVTHF"
     gene            15606..17027
                     /gene="cysS"
                     /locus_tag="ACSIGJ_000987"
     CDS             15606..17027
                     /gene="cysS"
                     /locus_tag="ACSIGJ_000987"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HHQ1329876.1"
                     /translation="MTAIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELNARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAEFDLSENANGYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAHL
                     AGCLKALGGIIGLLQRNPIEFLQGGAVSDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDSAGGTTWRRG"
     gene            17198..17977
                     /locus_tag="ACSIGJ_000988"
     CDS             17198..17977
                     /locus_tag="ACSIGJ_000988"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HHQ1329877.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGYWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LGVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVMHTLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            17964..18308
                     /locus_tag="ACSIGJ_000989"
     CDS             17964..18308
                     /locus_tag="ACSIGJ_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329878.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAVFMLSRM
                     QGWLAPTKANPIWALVWLLICLPCLLIAAKCLGWKGWRRVVNIFVCLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            18318..18848
                     /locus_tag="ACSIGJ_000990"
     CDS             18318..18848
                     /locus_tag="ACSIGJ_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHQ1329879.1"
                     /translation="MESFELEDLTLWLIRDADEAEMWIDRWAVSYPVVQMSEASANGS
                     TEAWQSALQTAFERIQGRHIAVVAHGAGAAAFLAWLYRADILTQKKLAGIILVSPRPD
                     IFPDDAEHTFQRVRCPCRAALVVSEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(18941..20056)
                     /gene="asd"
                     /locus_tag="ACSIGJ_000991"
     CDS             complement(18941..20056)
                     /gene="asd"
                     /locus_tag="ACSIGJ_000991"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HHQ1329880.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTSSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIALDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAIHAQVADELADPSSAILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSGNPTVIDGLCVRIGAMRCHS
                     QAITLKLKKDLPVSEIEAILAGANDWVKVVPNEKEASIRELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPMRRVLRIVLGSL"
     gene            20231..20812
                     /locus_tag="ACSIGJ_000992"
     CDS             20231..20812
                     /locus_tag="ACSIGJ_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HHQ1329881.1"
                     /translation="MEEKNDYTDAVSDNRNGQEIEVGIAGAGDRILAALLNQLFTFLI
                     LLVPFVGLIAFAVKNEGRIGGREEIFGLLLGMTSFWVGLAGILAYTVIQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGENGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(20874..21728)
                     /gene="folD"
                     /locus_tag="ACSIGJ_000993"
     CDS             complement(20874..21728)
                     /gene="folD"
                     /locus_tag="ACSIGJ_000993"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HHQ1329882.1"
                     /translation="MSAQLINGKEVSQKHLQAIAEAVAQRQQDNLHTPCLAVVLVGGD
                     PAGAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAILERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAAADILVVGVGIPNFVKG
                     GWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            21951..22028
                     /locus_tag="ACSIGJ_000994"
     tRNA            21951..22028
                     /locus_tag="ACSIGJ_000994"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21986..21988,aa:Pro,seq:tgg)
     gene            22053..22129
                     /locus_tag="ACSIGJ_000995"
     tRNA            22053..22129
                     /locus_tag="ACSIGJ_000995"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22087..22089,aa:Arg,seq:tct)
     gene            22157..22232
                     /locus_tag="ACSIGJ_000996"
     tRNA            22157..22232
                     /locus_tag="ACSIGJ_000996"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22190..22192,aa:His,seq:gtg)
     gene            22444..22950
                     /gene="rfaE2"
                     /locus_tag="ACSIGJ_000997"
     CDS             22444..22950
                     /gene="rfaE2"
                     /locus_tag="ACSIGJ_000997"
                     /inference="COORDINATES: protein motif:HMM:NF013621.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HHQ1329883.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSAGAALVLALNTDASVRRLGKGGDRPVNSLENRAAVAAALESVDLVTWF
                     DEDTPAALIEAVKPEVLVKGGDWVVDKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            22947..24725
                     /locus_tag="ACSIGJ_000998"
     CDS             22947..24725
                     /locus_tag="ACSIGJ_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HHQ1329884.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLAREADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRDLGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWAFDRPQYELGSLSPVAA
                     LACRRALGCLGLETQIKWPNDLVVGRDKLGGILIETVRAGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELGAVLEQYAEEGFAPFLNEYETANRD
                     HGKAVLLLRDGETVCEGTVKGVDGRGVLHLETAEGEQTVVSGEISLRPDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGESKKAQVKEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRPAGKRYPFPTTT
                     GNAVASGMMDAVCGSIMMMHGRLKEKNGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHA"
     gene            24838..>25656
                     /locus_tag="ACSIGJ_000999"
     CDS             24838..>25656
                     /locus_tag="ACSIGJ_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HHQ1329885.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKAAGGVPENRAVENTPPA
                     TPAAGNAAASVEDTAALLKPGDILSEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLADAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSC"
BASE COUNT         5683 a         6421 c         7385 g         6167 t
ORIGIN      
        1 tgaaaaacaa attgtatcaa aattgtaaat ttaatccact ataatctcca tataataaat
       61 ggctgtctga agcccatatg cgactatatc tgccgaccct atggtcaatc atcaatcagc
      121 agtggaccag tgtgccttcg tcttcgccgg taatgacgcg tttgagcgag ccttgtttgg
      181 cgatgccgaa gacgacgata ttgagcttgc gttcgcggca gagggcaaag gcggtcgcgt
      241 ccatgacttt gaggttttta ttcaaggctt cgtcaaaagt aatggtttcg tagcgtgtgg
      301 cggacgggtc ttttttcggg tctgcggtgt acacgccgtc gacgttggtg gctttgagca
      361 tcacgtcgca gttcatttcc gcgccgcgca atgcggcggc agtgtcggtc gtgaagaacg
      421 gattaccggt accggcggcg aaaatcacga ctttgccttc ttccaaatat tgaatggctt
      481 taggacgggc gtaagtttca gcgatttgct gcatagacag tgcggattgt acgcgcgctt
      541 tgatgcccaa ggtttcaaat gcgtctttga gtgccaaggc gttcatcacg gtcgccatca
      601 tgcccatgta gtcggcagtg gcgcggtcca tgctgcctgc ttgtgcagat acgccgcgga
      661 aaatgttacc gccgccgaca acgataccga cttgcacacc cattttcacg atttcggcaa
      721 tttcgccgac agtttgaaca atggtatcgt gattgatgcc gaacggatcg gaacccatca
      781 gggattcgcc ggagagtttc agtaatacgc gtttgtattt gatttgctgt gtcatgggat
      841 accttgcttt cttgaatgtc gtcggtcgga gttgggacgt ctgaatttca aatggtcgga
      901 ttacaatgat atggtgtgtc gttctattat tgttttgttt tcagacggcc tttatattcg
      961 gtcgtcttaa aataggtacc gtaaacggtt ttctcaaaaa aaagcaccct gattcgtttg
     1021 gaatccaggt gctttctttt tcataagtgc cttacacttt ggcggctgcg gctacttcgg
     1081 ctgcgtagtc gacaacggct ttctcgatac cgtcgcctac tttgtagcgt acgaagctaa
     1141 ccacttcagt gtcgttttct ttagcgaatt gggcaacagt ttggtcaggg ttcatcacga
     1201 atgcttggcc gttcagagtg atttcagcca agaatttgcg gatacggcct tcaaccattt
     1261 tagcggcgat gtcggcaggt ttgccggaag cgatggcttg ctcggtgtag atgtggcgtt
     1321 ctttttcaac ggtttcagca tctacttcgg cttcgcttac gcattgcggt ttggcggcaa
     1381 cgatgtgcat accgattttg cgtgctacat cttcagagcc tttgtactcg accaaaacgc
     1441 cttcggtcgc caatgcgccg tggatgtagg caaccagttg gttggcagtg tcaatcactt
     1501 ggaagcggcg gacagacatg ttctcgccca atttagcgat gatggctttg cgttctgatt
     1561 caaccagttc gctcagttct tcaacagaag ccggtttttt ctcggcagca gttttcgcaa
     1621 cgaagttggc aaattctacg aagccggcgt ctttagcaac gaagtcggtt tcgcagttta
     1681 cttcgaccaa tgcgccgaca ttaccgttga ttgcataagc caatacgcct tcggcagcag
     1741 tacggccagc cagtttgccc gctttcgcac cggatttaat acgcaggatt tcttcggctt
     1801 tgtcgaagtt gccttcagct tcaaccaagg cttttttgca ttccatcata cccaggccgg
     1861 tagcggcgcg caggtcggca accatttttg cagtaatttc tgccattttg aatctcctag
     1921 atttttggga aatacggaaa ggattttcca cattcgggaa tcgtgtgccg cgaacgggcg
     1981 ttcagacggc atatttgaga aaaggggcat aacgcccctc ttctgtgtac cgaattactc
     2041 ggcagcggtt tcttgggcag cggctacggt ttcttgcagc gcttggtttt tgccttccaa
     2101 aactgcgtcg gcgatgcctc ggcagtacag gcggatggct ttggcggagt cgtcgttgcc
     2161 ggggatgacg tatttcacgc cgtctgggct gttgttggta tcgactacgg cgataacggg
     2221 gatgcccagt ttttcagctt caaccagagt acctttttgg tagccggtat cgataacgaa
     2281 aatcgcgtcg ggcaggcctt tcatgttttt gataccgccc aaagagcgtt ccagtttttc
     2341 aacgtcgcgt tgcatttcca gaatttcttt tttgctgaaa ccgctttcgg cagcattttc
     2401 caaggctgcg gttttttctt ccaggcgttt gatggattgc ttaacggttt tgtagttggt
     2461 cagcataccg cccaaccagc ggtggtcgac gaaaggcata ccggcgcggg tcgcttcttc
     2521 gcggatgatg tcgcgtgcct ggcgtttggt acctacgaac aataccgtac ctttgttggc
     2581 aaccagacga cgtacggctt cttgcgcctc ttggaacatc ggcagggttt tttccaagtt
     2641 tacgatatgg attttgttgc gcgcgccgaa aatgtattga gccattttcg ggttccagaa
     2701 acgggtttgg tgaccgaagt gaacaccggc ttcaatcatc tgacgcatag taatttgaga
     2761 catgttattt ccttgaaagg gttaaagagc acacattcaa tcgcatagaa cttgataggc
     2821 ggcgcctatt gtcaagcacc cttcggcgaa agtgggcata agttttaaaa atagtgtgtt
     2881 tccgaaatgg aaaactgacg gattatagcg gataagatgc cgtctgaaaa gggattcagg
     2941 gttttgtgcc gctgtctttt tgccgttgtg cgcgctgctg ttcggattct gcctgttcct
     3001 gctgccccat caggcggttg cggcggcgga cggttacggc gaagaccgtg aacacggtgg
     3061 gcagaaccac ccaaaaaacc aaataaatca aagcgcgcgc aatactcggc tgcgcggcgg
     3121 aatacataac ggcaacaaaa agatagccga tggcgacgat gtgccacatt gtgtgcgtcc
     3181 tgtttgaatg ttaacaaaat cgttataatc gcaacattct accgcaatcc tgaaaggccg
     3241 tctgaaatga ggaacgagga aaaacgcgcc ctgcgccgcg aattgcgcgg gcggcgttcg
     3301 caaatggggc gggacgtgcg ggcggcggcg gcggtaaaaa tcaaccgcct gctcaaacgt
     3361 tatatcaagc gcggtcggaa aatcggcgtg tattggccga tgggcaagga attgcgtttg
     3421 ggcggctttg tccgcgcggc gcaaaaacgc ggcgcaaaac tctatctgcc ttatatcgaa
     3481 ccgcacacgc ggcggatgtg gtttacgccg tatcctgaac gcggaatgga acgggaacgc
     3541 aagcgcggta gggcgaagct gcatgtccct cagtttgcag ggcgcaaaat ccgcgtgcac
     3601 ggtttgtcgg tattgctcgt cccgcttgtc ggcatagacc gcgaaggcta ccgtttgggg
     3661 caggcaggcg gctattacga tgcgacgctt tcggcgatga aataccgttt gcaggcgaaa
     3721 accgtgggcg tgggctttgc ctgccagttg gtggacaggc tcccacgcga ggcgcacgac
     3781 ctgccgctgg acggttttgt atcggaagcg gggatattgt gtttttagcc gttcagacgg
     3841 catttcgggc ggaaatgccg tctgaaggcg gaatattggg agaaaaggat gtcggaaagg
     3901 ataatactgc cggttttgtc attgggcggc gtacggctcg aaccgttgga tgtgcatcat
     3961 gaaacgggtt tgcgcgaggc ggtttgcgat ggggaggttt ggaagctggg ggtaacgtcc
     4021 gcgccccatc cggatagggt ggcagactat atcgggacag cattggcaac gcgtttggcg
     4081 tttgcggttg tcgatgaaga ggcggacagg gtggtcggga caacggcgta ttatcatttt
     4141 gaaccgcaga ttccgcgttt ggatatcgga ttcacatggt atgcggcatc ggcacggcgg
     4201 aaacggataa acacctgttg caaaatcatg ctgttggatt acgcatttga tgttttagct
     4261 tgctgttgcg cgggatggcg cacggatatt ctgaaccttg cctcgcaacg tgccatcgaa
     4321 cggctgggcg cggaaaaaga cggcgtgctg cgtatgcata tgctccgtaa agacggcagt
     4381 gtgcgcgata cggttgtgta cagtatgctg agggaagatt ggtgcaaaaa cagggaaatc
     4441 ctgaccggca ggttggcagg gtatggcgtg caggtgtgag gcagtattct caaatttaaa
     4501 aaacttaacg cgatatgctg acaatctttt aaaaattaat atataaacaa ttttgatttt
     4561 ttgtctatta aaaatttgat atatatagat aaataactat ttttattttt tgtttcgcag
     4621 ttccccggct tggcgggcga cagaaaaact ttgcatttcc cagtaaaatc cactacattc
     4681 acgataacat attacaagcc cctgaagcgg tgtcggaagc catacttgca acgcattcca
     4741 accccgaaac agcgtgctcg gacacacacc attgccggtt tcccctgctt ttgcgattca
     4801 ggcgtttgta gtacggcaaa taaccataag ggcagaagat gcctgttttt taggagtaaa
     4861 ccatggctga agcgacagac gttgtcttgg tgggcggcgg cattatgagc gcgactttgg
     4921 gcgttttgct caaagaactc gaaccgtctt gggaaatcac cctgattgaa cgcttggaag
     4981 atgtggcgtt ggaatcgtca aacgcgtgga acaacgccgg cacggggcat tccgcgctgt
     5041 gtgaattgaa ctatgcgccg ttgggtgcaa atgggattat cgatccggcg cgcgccctca
     5101 atattgccga acagtttcat gtcagccgcc agttttgggc gacgctggtc gcggaaggca
     5161 agttggaaga caattccttc atcaatgccg tgccgcatat gtctttggtg atgaatgaag
     5221 accattgttc ttatcttcaa aaacgttatg acgcgtttaa aacccaaaaa ctttttgaaa
     5281 atatggaatt ttccaccgat cggaacaaaa tttccgattg ggctccgctg atgatgcgcg
     5341 gccgggacga aaaccaaccc gtcgccgcca actactccgc cgaaggtacg gatgtcgatt
     5401 tcggacggct gacgcgccag atggtgaaat atttgcaggg caagggcgta aaaaccgagt
     5461 tcaaccgcca cgtcgaagac atcaaacgcg aatccgacgg cgcgtgggtg ctcaaaaccg
     5521 ccgatacccg caaccccgac gggcagctca ccctccgtac ccgcttcctc ttcctcggcg
     5581 cgggcggcgg cgcgctgacc ctgctgcaaa aatccggcat ccccgaaggc aaaggctacg
     5641 gcggcttccc cgtgtccggc ctgttcttcc gcaacagcaa ccccgaaacc gccgaacaac
     5701 acaacgccaa agtgtacggg caggcttccg tcggcgcgcc gccgatgtcc gtcccgcacc
     5761 tcgacacacg caacgtggac ggcaaacgcc accttatgtt cggcccttac gcaggcttcc
     5821 gttccaactt cctcaagcaa ggctcgctta tggatttgcc gctgtccatc catatggaca
     5881 atctctatcc tatgctgcgc gccggctggg cgaatatgcc gctgaccaaa tacctgctgg
     5941 gcgaattgcg taaaaccaaa gaagaacgct tcgcctccct gctggaatac taccccgagg
     6001 caaaccccga cgactgggaa ctcatcaccg cagggcaacg cgtccaaatc attaaaaaag
     6061 actccgaaaa aggcggcgtg ctccagtttg gtacggagat tgtcgcccac gccgacggct
     6121 cactcgccgc attgctgggc gcgtcgccgg gcgcatcgac cgctgtgccg ctgatgatcc
     6181 ggctgatgca ccaatgcttc cccgagcgcg ccccgtcttg ggaaggtcgt ctgaaagagc
     6241 tggtaccggg ttacggcatc aagttgaacg aaaaccctga aagggcggat gaaattatcg
     6301 cctataccgc gaaagtgttg gatatttaag ccgaatcttt caagaaaagc cgtaccccca
     6361 tatggtgttg atgccgtccc tgccggttat gcccttcatc cggcagggca ggttttgata
     6421 cacggggcag gtaatcgttt tttccctttt gcgaataatg gaacggtcaa aaaacagcgt
     6481 tttcagaaat gaaaacgctg ttttttgttg cctgattaac gtggggaaca gtctttgaag
     6541 acgatttggt tgtcaggtgc ggtaatcata ataggctgtt tgcggtagga tttgctgtcc
     6601 attgcgccgg tgctcagcac ataaccgcct tctttgccgt agaacgtgtc catattgtcg
     6661 gatttatcca aattgatggg catttgcacg cgtttgccgt tgatgacggc ggaagcgtat
     6721 gtggtcaggc cctgtttgtt gaagccgtag gttactttga cttttttacc ttgctggcag
     6781 acgtagctga cggttttttt ggcaacggtg gggttgttcg tgccggcagc agcagccata
     6841 ctgctgagcg cgattgcgga agacaggatt gcggtggtca gaagtttcat ttttattcct
     6901 ttggatggat gtggagtttg cccgtttcga gctgtttgac attctaatgt cggcgtatcc
     6961 tgaaataaac aggaaattgg ataaagtttg taaaaaggcg ggttcgggcg gttcggcggt
     7021 ttgaggcagg ggcggcaggc gggatacggc ggcctgtccg gtgtttcaga cggcattgtt
     7081 tgcgtttttc ggaaatgaaa aaccttcggg catccggagg ttgcggttta actttgtttt
     7141 tttctttcgg acttggtctg tttggacaat tcggcataca ggtcgggatg gtgttttttc
     7201 aacagggcgg cgatttccat atcttttttg ttgactttcg ccaaatcgat gcattcgata
     7261 tggacgaggc ggagcagttc gcgtacgggt ttgggcattt tgcggccggt ttcgtagcgc
     7321 gagcctccag actgggtaac gccgaccttg ccccagaagt ctatctggtt cagcccggtc
     7381 tttttacgga tggctttaac atcttctata ttttcaaata ttttcataaa acttccttta
     7441 agtttgtctg gttgcggggc aaattccgat ttgctttgtt ttcgtaatgt gccgggtcaa
     7501 gcctgttttt atattgcata cagcttcagg gtttgccggt ggcggggctg acggtgagga
     7561 tttcgtagcc ggtttcggtc accaagactt cgtgttccca ttgggcggag agggagcggt
     7621 ctttggtaac caccgtccag ccgtcgttga ggatacgcag gtggcgtttg ccttggttga
     7681 tcatcggttc gacggtaaaa atcatacccg gttttagaac gggaccctgc cctttttttc
     7741 cgtagtgcaa cacttgcggg gcttcgtgga aaccgcgccc gatgccgtgt ccgcagaatt
     7801 cttgtacaac cgaataacct gcattttcgg caacctgctg gcaggcgtaa cctacgccgc
     7861 ccagcgtcgc gccaggtttg acggcttcta tgcccgccat catagaggcg tgggttacgt
     7921 cgatcaggcg ttgggcgatg ggggagactt tgccgacggt aaacatacgg ctggagtcgc
     7981 cgtggaaacc gtcttttttg atggtgaggt cgatgttgat aatgtcacct tctttgagcg
     8041 gcttgtcgtc gggaatgccg tggcagatga cgtggttgac ggaggtgcag caggatttgg
     8101 ggtagggcgg gttgccgtag tggagggggg cgggatagcc gccttggacg ttgacgtggt
     8161 agtcgtaaac aagtttgtcg atttcgtcgg tggtgatgcc gggtttgacg aattgtccga
     8221 tgtagtcgag ggcttcggcg acgagtttgc ccagctcgcg cattttttcg atttcttcgg
     8281 gggttttgat gatgatgccg ttcataatgc ttccttggat tgcgggtgtg cctgccgtgc
     8341 cgggtcaaaa gaggttgtcg agaatctctt tatggggttt ggccggcgtg tttttcgggg
     8401 tgtccggttt ggtatggttt tctttgggcg tttctttttc tttgggtgtt tctttgggct
     8461 gcgcctgttt ttcgggtgtt tcttttcggg gttcgggatg gggggcgttt gtgggcagta
     8521 cgggggtttc tttgagtgtg ttttcagctt gtgttccggt gccggttaac gaatcggctg
     8581 cccgggccgc atccgaaggg cgcggtttga cccggttttt cttgggtgcg gcaggggacg
     8641 gcagtccgct gccgctttct tctgtgccgt ccggctgtgc gggttcggat gaggcggcgt
     8701 ggtggtgttc gggttgggcg gcatcttgtc cgacggcgcc gtttggcagc cagaattcgg
     8761 tttcgcgggg gctgtcggtg tgttgcggtt cggtttggag ggttttgtcg ccgttcgggt
     8821 tgaatgtgct gacgatgcct actgccaatg cgccgatggc ggtgatacag atgagcaata
     8881 cggcgcgtat caggagtttg ggggtcggcc tgaagggttt gttcggtttt tttgccattt
     8941 tgattgtgct tttggggtgt cgggcaatgc cgtctgaagg cggttcagac ggcattgccg
     9001 agtcagcgtt ggacgtagtt ttggtagagg gtaatgactt tttgtacgcc gacggtggtg
     9061 ctgacttttt gggtaatctg cgcctgttct tcgggggtga ggatgcccat aacgtaggtt
     9121 acattgccgt aggtaatgat tttgacgcgc gcctgtgtag cggggctgat gcccagcagc
     9181 gtggcgcgga ctttggacgt gttccaagtg tcgccggcga tgtcgcccgc agtgcgcggc
     9241 agggaggcga cggtaatgta gttgtatacg ccttcggcgg cctgttcgga acgtgcaatc
     9301 tgaccgacga actgtttttc gccttcggtg gcgacttgtc cgagcagcag caggtggcgg
     9361 ttgtagccga cgacggagat ttggggcgtg tagcctttgg tttggttgtt ttggcgcagg
     9421 taggaacggg cggtggtttc gatacgcaac gccataacgt tgtcatcggt ttgcgcgccg
     9481 gtggttcggc ggtcgatgac ggatttcgcg ccgacggcgg ccccgccgac gactgcgctg
     9541 aagcagccgc cgagggcaag gctgaggacg gcggcaatca gggtgcggac ggtgtgtggt
     9601 ttgggtttca tcggggactt cctttcttgg gcgtttcaga cggcattgct ttgcgccatg
     9661 ccgtctgaaa agggttacat tccttccagc agtacggagt cgatacagtc gcacatggcg
     9721 tgtatcagca ggatgtggtt ttcctgaatg cgggcggtgc gcggatgggg gacgttgagc
     9781 agaacgtcgg tgtctttgag catggcggcg attttgccgc cgtcgcggcc ggtcagggcg
     9841 atgacgtgca tatcgcgttc gtgcgcggct ttgacggctt cgatgacgtt ggcggaattg
     9901 ccggaggtgg aaatgccgac gaggacgtcg cctgcacgtc cgagcgcgcg cacttgtttg
     9961 ctgaatacgt ggtcgaaacc gtagtcgttg ccgatggcgg tcagcgcgga ggtgtctgtg
    10021 gtcagggcga cggcggcgag ttccatgcgt tctttctcaa aacgcccggt catttcggcg
    10081 gcgaagtgtt gcgcgtcggc agccgaaccg ccgttgccgc aggccaggat tttgccgtcg
    10141 ttcatcaggc attgcagcat cagctcggca gcctgtacgg tcggctcgac cagtattttt
    10201 tcggcttcct gtttggcacg gatgctttcg gcaaaatgtg cggcaacgcg ttcttgtaat
    10261 gtcgtcatat cgtcaacctg taatattctt tatccactcg ggcgggcggt ttccttcgat
    10321 aagtaccgca tcgaggcggc acggtacgtt tgtcagcctg ttctgttgca gataatactc
    10381 tacacttcgt tgcagtttca ataatttgga cggagaaatg ctgtatgccg cgccgccgaa
    10441 ccgttggttt ttgcggtatt ttacttcaac aaacagaatc atgccgccgt ttttgacaat
    10501 caggtcgatt tcgccgtagg cgcagtgcca gttgcgggca agcagcgtgc agccttggga
    10561 ttggaggaag gcaagtgcgg catcttcccc tgccgtaccc tgtttgtggt ttaagcgcat
    10621 aatcggttta acctttataa tgtctgtttt tcagacggca ttttttatgt ttcagaaaca
    10681 cttgcagaaa gcccccgaca gcgtcgtcgg agggacatta tacgtggttg ccacgcccat
    10741 cggcaatttg gcagacatta ccctgcgcgc tttggcggta ttgcaaaagg cggacatcat
    10801 ttgtgccgaa gacacgcgcg ttactgcgca gcttttgagc gcgtacggca ttcagggcag
    10861 gttggtcagt gtgcgcgaac acaacgagcg gcagatggcg gacaaggtaa tcggtttcct
    10921 ttcagacggc ctggttgtgg cgcaggtttc cgatgcgggt acgccggccg tgtgcgaccc
    10981 gggcgcgaaa ctcgcccgcc gcgtgcgcga agcagggttc aaagtcgttc ccgtcgtggg
    11041 cgcaagcgcg gtaatggcgg cgttgagtgt ggccggtgtg gcggaatccg atttttattt
    11101 caacggtttt gtaccgccga aatcgggcga acgtaggaaa ttgtttgcca aatgggtgcg
    11161 ggcggcattt cctgtcgtca tgtttgaaac gccgcaccga atcggggcaa cgcttgccga
    11221 tatggcggaa ttgttccccg aacgccgtct gatgctggcg cgcgaaatca cgaaaacgtt
    11281 tgaaacgttc ttaagcggca cggttgggga aattcagacg gcattggcgg cggacggcaa
    11341 ccaatcgcgc ggcgagatgg tgttggtgct ttatccggcg caggatgaaa aacacgaagg
    11401 cttgtccgag tctgcgcaaa atgcgatgaa aatccttgcg gccgagctgc cgaccaagca
    11461 ggcggcggag cttgccgcca agattacagg tgagggcaaa aaggctttgt acgatttggc
    11521 actgtcgtgg aaaaacaaat gatgccggat ggaccggcac catagtgcga acggaatcaa
    11581 cggacacgcc ccgtcatgcg gtacagcacg tctttgcctt gaacacgatg gacgacgacc
    11641 atggcgacgt gtccgacgac ggcggcaaac agcagccagc ccaaattgcc gtggaacgtg
    11701 ttgcccaagt ttgccatcca ctcgattttt tccggcgaac cctgcattac ttcaacgccg
    11761 aacactttca acgggccgcg tccgccaccg tattggcgga tcatgccgat aacgggaacg
    11821 gcaagcatga gcagatacag aatgccgtgt cctgccgctg ccgccttgct gtcgctttgc
    11881 ggacgcttgg cgcggttggc aaccgcccac acgatgcgca atgcaatcac cgtcagcatg
    11941 aggaaaccga aagatttgtg caggccgaac aggctgcccg cccattccgc ctcttcgtaa
    12001 attgtccaca ggacaatggt ggtcagaatg ccgatgaagc cggcaacggt cagccagtgc
    12061 agcgcgcgtg tcaacgggtc gtaaacggca ggttggtttg tgttcataaa aaaatcctta
    12121 tcatatgtaa cggtttgaac ggacattccg tcgggtcgga atgtcaaaaa ggcgcgattg
    12181 taccaaagaa gttggacata ttttgtttgc aggctgaaga tttgcttaaa aattcattaa
    12241 gatgggcaga acaaatagct tctccgcggt atattgaaat accgcttata ccttataatg
    12301 tcggcagaaa atctgttcag acggcatcaa aacatatggc aatccgcctg aaaccctatt
    12361 taaaaccaac aaacaagagc atttaatgaa attcatcgac gaagcaaaaa tcgaagtcgc
    12421 cgcaggcaaa ggcggtaatg gcgcaaccag tttccgccgc gaaaaattcg taccccgcgg
    12481 cggcccggac ggtggcgacg gcggcaaagg cggcagcgtc tgggcagaag ccgacgaaaa
    12541 caccaacacc ctcgtcgaat accgcttcgt caaacgctac caagccaaaa acggcgaaaa
    12601 aggccacggt tccgaccgct acggcgcagg tgcggacgac attgtcctca aaatgcccgt
    12661 cggcaccctt atccgcgacc tcgacaccga cgaaatcgtt gccgacctca cctaccacgg
    12721 acagcgcgtc tgcctcgcca aaggcggcaa aggcggcttg ggcaacatcc acttcaaatc
    12781 ctccgtcaac cgcgccccaa aacaatccac gcccggcgaa gaaggcgaaa cccgttccct
    12841 gcaactcgaa ctcaaagtcc tcgccgatgt cggcttgtta ggcatgccca acgccggcaa
    12901 atccaccctg attaccgccg tctccgccgc acgccccaaa atcgccaact acccctttac
    12961 caccctgcat ccgaacttgg gcgtggtacg catcgacgaa aaccacagct tcgtgatggc
    13021 cgacatcccc ggcctgattg aaggcgcggc agaaggcgca ggcctcggcc accgcttcct
    13081 caaacaccta tcacgcaccg gtctgctgtt acacgttgtc gatttggcac ccttcgacga
    13141 aaccgtcaat cccgccgaag aagcactcgc catcatcaac gaattacgca aatacgacga
    13201 agaactttac ggcaaaccgc gctggctggt gctgaacaaa ctcgatatgc tcgacgaaga
    13261 agaagcccgg gcgcgaacag ccgccttcct cgaagccgtc ggctgggact accccgaacc
    13321 tgatgaccgt ttccaattcg acatggaaac cccgcgcctc ttccaaatca gcgcgctgac
    13381 acatcaaggc acgcaggagt tggtacacca aatcaaccaa tacctggccg agaaaaaacg
    13441 tatcgaggct gaaaaagccg aagcggagaa agcagcggca aatgttgaaa ttatcgaaca
    13501 gcagcctaaa acggatactg gcgtgtttaa gccggagtaa aaggacaagg ccgtctgaaa
    13561 gttttcaggc ggctcattct gcagagatgt gctatgtaca aaacaataga tttattttca
    13621 ggcattggcg gaatacgcct aggctttgaa aaatacgggt gcaccaatgt attctcatcg
    13681 gaatgggaca aatatgcccg acaagtgtat gaagcaaact ttggagaaaa accttttggc
    13741 gacattaacg gcatcgatcc ttcagatatt cccgatcatg atattctgct tgcgggattt
    13801 ccgtgccaac ctttcagtat cgcaggtaag ggattgggat ttgaagatac gcgcggaaca
    13861 ttgtttttca atattgccga aatcctgaaa accaaacagc cgaaggcttt tcttctggaa
    13921 aacgttaagc ggctgactac gcacgattcg ggcaggactt tccgtattgt tttagaaacc
    13981 cttaaacagc ttggttatac ggtttatttt aaggttttaa atactttgga tttcggattg
    14041 ccgcaaaaac gcgagagaat ttatattgtg ggtttttcag acaacatccc tttttacttt
    14101 cccgaaccca taaaccaata tcggccattg ggcgaactgc tggaaaacga tcgagatgtc
    14161 gaaccaagct attttttatc agataccttg aaacaaaaac gtttggcagc cttaaaaaaa
    14221 gctccgccca ccccatcaat ttggcatgaa aatattggcg ggaatgtttc cgcacttcca
    14281 tattcctgtg ccttaagggc cgggggaagt tacaattatt tggtggtaaa cggagtaaga
    14341 aggctaacag gaagggaaat gttaagatta caaggttttc ccgatgattt tgaaatcaat
    14401 atcccctact cgcaagtcag gaaagtcgcc ggaaattctg tatccgtccc tgtaatagaa
    14461 gccatcgcgg aaaatatgct cgcttctctt tccggcaagg tcgaacaaaa agggcaattg
    14521 gatttattgg aggcaggatg acgcttgaag aacaacaagc caaagaagca ttggacggca
    14581 ttatcaaaaa atcccgtgtc cacctttata aacccattca aattgcagaa attttatatc
    14641 atgaccgttg tataaaacag ttggattttt taaatttaga tacttaccgc aaccaatcta
    14701 aacgctggag ggatgaaatc tgccgtcggt ttttagggcg gatttctact tcatctgcaa
    14761 aattccaaga taatctgttt gaaaaaaatg caataccgcc tgaaaaacta gcggtattgg
    14821 gaacattaaa cagacagtcg gacggcgggg tagaatcgta catttataaa cagtttttca
    14881 atcgtttttc ccaaatgagc gaagcattgg cttatgtcgg caatacagac agatactcct
    14941 tccaactgtc tgaatttcta aatttatttt ggctcgagcc gggattgaaa agaagtatag
    15001 acaagatata tgaaatcgtt gtttatgcgt tgtttgatgc attggtttca gaattaggca
    15061 taacggtttc aattgatttt cctaaggaaa acctgttttt atgggaggaa tatcaagatt
    15121 ttgccgaaaa aatcatcact atgccgaaaa atgagcattt aaaacttcct gcaaaaatcc
    15181 atcgtgtagg cgtaaccaat gctgccgata gagggttgga tatgtggtct aacttcggat
    15241 tggccataca ggtcaaacat ctctctttag acgaagagtt ggcagaagat attgtatcct
    15301 ccattagcgc agatagaatt gtcattgtct gcaaaaaggc agaacaatcg gtgattgttt
    15361 cattactgac gcaaataggc tggaaaagcc gaattcaaaa catcgtaact gaggatgatt
    15421 tgataagttg gtacgaaaaa gcattgagag gccaatatcc gattgcagaa gcgttgttgg
    15481 aaaatattaa aactgaaatt atgcgtgaat ttcctgccgt taatgaagcc aatgaatttt
    15541 tagatttcgc ccaaaatcgc ggatatgaca ttactgttac tcatttctaa acttacttga
    15601 tgaatatgac cgccatctac aacaccctta cccgtcaaaa agaacccttc gcccccatcg
    15661 accctgaaaa cgtgcgtatg tacgtctgcg gcatgaccgt ttacgactat tgccacttag
    15721 gccatgcccg tgtgatggtg gtgttcgaca tgattgcccg ctggttgcgc gagtgcggtt
    15781 atccgctcac ttatgtgcgc aacatcaccg acatcgacga caaaatcatc gcccgtgcgg
    15841 ctgaaaacgg cgaaaccatc ggcgaactga acgcgcgttt cattcaggcg atgcacgaag
    15901 atgccgatgc tttgggcgtg ttgcgtcccg acatcgagcc gaaggcgacg gaaaacatcc
    15961 cgcaaatgat tgccatgatt gagaccctga ttcaaaacgg caaggcatat cctgccgcaa
    16021 acggcgacgt ttactacgcc gtgcgcgagt ttgccgctta cggacaatta tcgggcaaat
    16081 cgttggacga cctgcgtgcg ggcgaacgcg tggaagtgga cggtttcaaa cgcgatccgc
    16141 ttgattttgt attgtggaaa gcggcgaaag caggcgaacc ggcgtgggaa agcccgtggg
    16201 gcaacggccg tccgggttgg cacatcgaat gttccgcgat gagtgaaaac ctgttcggcg
    16261 atacgttcga cattcacggc ggcggcgcgg atttgcagtt cccgcaccat gaaaatgaaa
    16321 tcgcccaaag cgtcggtgcc agcggtcaca cctgcggtca tgaccacgcg caaacccacc
    16381 acggtcaaag tattgccagc cacgtcaaat actggctgca caacggcttc atccgtgtgg
    16441 acggcgaaaa aatgtccaaa tcgctgggca acttctttac tatccgcgaa gtattgaaac
    16501 aatacgaccc cgaagtcgtg cgtttcttca tcctgcgcgc ccactaccgc agcccgttga
    16561 actactccga cgcgcatttg gacgacgcga aaggcgcgtt gacccgtctg tacaccacct
    16621 tgaaaaacac accggcggct gagtttgatt tgtctgaaaa cgccaacggc tacacccgcc
    16681 gtttctacgc cgccatgaac gacgatttcg gtacggtcga agcggttgcc gtgttgtttg
    16741 agctggcagg cgaagtgaac aaaaccaatg acgcacacct cgccggctgc ctgaaagctt
    16801 tgggcggcat catcggcctg ctgcaacgca acccgattga gttcctccaa ggcggcgcag
    16861 tttcagacgg cctctccaac gaagaaatcg aagacttgat tgcccgacgc aaacaggctc
    16921 gcgcggataa aaactgggcg gagtccgacc gcatccgcga ccttctgaac gaacacaaaa
    16981 tcattttgga agacagcgcc ggcggcacga cttggcggcg cggttgaact gtatcggaaa
    17041 gggcaaaagt accgcctttt gccctttttt cgttttcaga cggcatttcc cgcttgaagc
    17101 cccgccgttt tcagcaggca ggaccgaatg ggaaataccg ggcggcgcac cgggtgccgc
    17161 tttgtgtatg ggaacattgt gagaacttat ttttattatg cttaaaatca tttccgccaa
    17221 cgtcaacggc atccgctccg cctacaaaaa aggtttttac gaatacatcg ccgcatcggg
    17281 cgcagacatt gtctgcgtgc aggaactcaa agcgcaggag gctgatttgt ctgccgatat
    17341 gaaaaatccg cacgggatgc acggctattg gcattgcgcc gagaagcgcg gttacagcgg
    17401 tgtggcggtg tacagcaaac gcaaacccga caatgtgcaa atcggcatgg gcattgagga
    17461 attcgaccgc gaagggcggt ttgtgcgttg cgatttcggc aggttgggcg tcatttcgct
    17521 ttatttgccc agcggcagca gcgcggaaga acgccagcag gtgaaatacc gtttcctcga
    17581 tgcgttttac cctatgctcg aagcgatgaa aaacgaaggg cgcgacatcg tcgtctgcgg
    17641 cgactggaac atcgcccacc aaaacatcga cctgaaaaat tggaaaggca atcagaaaaa
    17701 ttcgggcttc ctgcccgaag agcgcgagtg gataggcaaa gtcatgcata cgctgggctg
    17761 gacggacatg tggcgcacgc tctatcccga tgtgccgggt tatacatggt ggagcaaccg
    17821 cgggcaggcg tatgcgaaag atgtcgggtg gcgcatcgat tatcagatgg ttacgcccga
    17881 acttgccgcc aaagccgtgt ccgcacacgt ttataaagat gaaaaattct cggatcacgc
    17941 ccctttagtt gtggagtatg actatgctgc cgaataaggt tttgggtaaa tatgattgga
    18001 atgtggacgg aaaaacagga ataggcgcgg catgggttgt cgctgcgttt atcctgccca
    18061 tattggtgtg ggcggtgttt atgttgtcgc ggatgcaagg ctggcttgcg ccgaccaagg
    18121 caaacccgat atgggcgttg gtgtggctgc tgatttgtct gccctgcctg ctgattgcgg
    18181 ccaaatgttt gggttggaaa ggctggcggc gggtggtgaa tatttttgtc tgcctgaccg
    18241 tctgcgccat attgagcgta cccgcttcgc tgctcatcgc ctttaccctc cgggacctgc
    18301 tcaaatagtc gcggacaatg gaaagtttcg agcttgagga tttgacgctg tggctgatac
    18361 gcgatgcgga tgaagcggaa atgtggatag accgttgggc ggtcagttat cccgttgtgc
    18421 agatgtccga agcgtccgca aacggcagca cggaagcgtg gcaaagcgcg cttcagacgg
    18481 cattcgaacg gatacagggc aggcatatcg ccgttgtcgc acacggcgcg ggcgcggccg
    18541 cattcttggc gtggctgtac cgcgccgaca tcctgacgca gaagaaactc gccggtatca
    18601 ttctcgtatc tccgcgtccc gatatttttc ccgacgatgc ggaacacact ttccaacgcg
    18661 tccgctgtcc ttgccgtgcc gcattggtgg tgtccgaaca cggcggcgtg ccgcacggtt
    18721 gggcgcaaaa acaggcggat ttgtggaacg cccgcctgtt ggtttccccg cattcgggca
    18781 gtttgaacgg tatgctcggc ggctggcagt ggggtatgaa gctgatgcag gaaatgttgc
    18841 tggcgtgaac ggtcgcttta tcggaatttc ggttaaccgg ggttgcaagg aaaataccgt
    18901 ctgaaacagg cttcagacgg catttccatt caaacagggc ttacaggctg cccaacacga
    18961 tacgcaacac gcggcgcatc ggttcggctg caccccacaa aagttggtcg ccgacggtga
    19021 acgcgctgat gtattcgccg cccatgccca gtttgcggat gcgtccgaca gggacggaca
    19081 gcgtgccggt aactttggcg ggcgtcagct cgcggatgct ggcttctttt tcgttgggga
    19141 cgactttcac ccagtcgttc gcgcctgcca aaatcgcttc gatttcggaa acaggcaggt
    19201 cttttttcaa cttcagagtg atggcttggc tgtggcagcg catcgcgccg atgcggacgc
    19261 acaggccgtc gatgacggtc ggattgccgc tgcgtccgag gattttgttg gtttccacgc
    19321 cgcccttcca ttcttctttg gactggccgt tgcccaaatc cacgtcaatc cacggaatca
    19381 ggctgccggc gagcggtacg ccgaagttgg ctttcggata gtcttcgctg cggaggaaat
    19441 cggacacttt gcggtcgata tcgagaatcg cgctggaagg atcggcaagc tcgtccgcca
    19501 cttgggcgtg aatcgcgccc atgccgctga tgagttcgcg catatttttc gcgcccgcgc
    19561 cggaagcggc ttggtaggtc atgctggtcg cccattcgac caaatcgttt tggaacaggc
    19621 cgcccaaagc catcagcatc agggagacgg tacagttgcc gccgatgtag tttttcacgc
    19681 cgtttttgag accgttgtcg atgacgttgc ggttgacggg gtcgagggcg ataatcgcgt
    19741 cgtctttcat gcgcagtgag gacgccgcgt caatccagta gccgttccag ccgctgtcgc
    19801 gcagggcttg gaagacggat ttggtgtaat cgccgccttg gcaggtgacg ataatgtcca
    19861 ttttcgccag ctcggcaacg tcgttcgcgt ccaataatgt tttagccgcc tgaccgaaat
    19921 caggtgccgc gccgccgacg ttggaagagg taaagaaaaa cgcttcggga atgtgggcga
    19981 agtcgttttc ttctttcata cgctgcatca aaaccgaacc gaccataccg cgccagccga
    20041 cgaaacctac tttcatgtat tgctccttga ggaaaatcgt taataaaatt atgtatggcg
    20101 taaagaaagc gttttaacgc cgtattggga aaatgtaaag ttctttatcg aattttcttt
    20161 ttgggattgg cgtttgcagc agtagggcgg tagaattcca tctgtttaag aacaattatc
    20221 aggaacggat atggaagaaa aaaacgacta caccgacgcg gtatccgaca accgcaacgg
    20281 gcaggaaatt gaagtcggaa ttgccggcgc aggcgaccgg atacttgccg cgctgctgaa
    20341 ccaattgttt acctttttga ttttgttggt gccgtttgtc ggactgattg ccttcgccgt
    20401 caaaaatgaa ggcaggattg gcggtcgtga ggaaatattc gggctgcttt tgggcatgac
    20461 ttccttttgg gtcggactgg cgggcatttt ggcgtacacc gtcatccaaa tctactatat
    20521 gagcagggac gggcagtcat tgggtaagaa aatcatgaga atccgcgtgt tgaaaaccga
    20581 cggccgcaat ccgggttttg tcggcacggt tttggtacgc gaaatcgcgt ggtcggtttt
    20641 ggttgccatt attgccgccg ttatcggtct tgcagtaggt gaaaacgggg aaaacgccat
    20701 caacctgctg gcattccttg ccaactttgt cctgctcttt atggtcaaac gcgaccgccg
    20761 cacgctttac gacatattgg cggatacggt tgtcgtcaag ctgcccaaat aaacggacgc
    20821 ggcaaaatgc cgtctgaaag gctttcagac ggcattttta tcttcagaac cgctcaagca
    20881 tcgtgcagcg aagctgcgtg caaagtgttt tccattaatg tggcaatcgt catcggaccc
    20941 acgccgccgg gaacgggcgt aatcatcgcc gcccgttctt ttgccgtttc aaattccacg
    21001 tcgccgcaca ggctgccgtc gtccaaacgg ttgatgccca catcgataac gaccgcgccg
    21061 ggtttgatcc acccgccttt gacaaagttc ggaatgccca cgccgaccac caaaatatcg
    21121 gcggcggcaa cttcatcggc aagattttcg gtcgcactgt ggcagaccgt taccgttgcg
    21181 cgcgccagca gcaattccaa cgcctgcggg cggccgacaa tattcgacgc gccgaccacg
    21241 accgcttttt tccccttcgg atcaatgccg taagcctcca aaagcgtcat cacgcccttg
    21301 ggcgtacacg ggcgcatcag cggcattttg accgccagcc tgccgacatt gtaaggatgg
    21361 aagccgtcca cgtccttatc cggcgaaata cgttccaaga tcgcttggct gtcgaggtgc
    21421 ttcggcagtg gaagctgaac cagaataccg tccacttcgg aatcggcatt cagacggtcg
    21481 accaatgcca gcagttcttc ctgcgatgtt gattcgggca gttcgtacga cagcgacttg
    21541 atgccgcatt tttggcaggc ggttttcttg ttgcggacat aaactgcgcc ggcagggtcg
    21601 cctccgacca aaaccacggc caggcaaggc gtgtgcagat tgtcctgttg gcgttgcgcc
    21661 accgcttcgg caatcgcctg caggtgtttt tgcgaaactt ctttaccgtt gatcagttgt
    21721 gccgacatat ccgtcctttt gttaaacagg tagaaaatgc tgaaaattat cgcattttcc
    21781 gtttgttttt acagcagaat gccgaaaata aatttgcccc gaaataaaat acggctgttt
    21841 ggacggatta aatatttttt caaatcaaaa aactatattt ttcatcttgg tgttttgaaa
    21901 ataaatgttg acgcatcttt ccctgaactg tatattccgc ttcttcaagt cggggcgtag
    21961 cgcagcccgg ttagcgcatc tgctttggga gcagagggtc gtgagttcga atcccaccgc
    22021 cccgaccaat caatcagcac gaatcggcat gagcgcccgt agctcaaccg gatagagcac
    22081 cgaccttcta agtcgggggt tacaggttcg attcctgtcg ggcgtgccaa gaatttgatt
    22141 gaaaaccgtt tttatggtgg ctgtagctca gttggtagag ccccggattg tgattccggt
    22201 tgtcgtgggt tcgagcccca tcagccaccc cagataaagg cccgtacttt tcggtacggg
    22261 tcttttcctg tttgcgcgta ctgtccgcgc cgtcgtaccg gtgcggcggt atgccgtctg
    22321 aaagccgggc cggtcatacg gaaaccgctt catacggcat ttgcgccgcc ggctttgcct
    22381 ttcgcatatg cttctgtatc atgtgggttt ttctgtcttt tatggttttt gtcggaggct
    22441 ttgatggttg acgcttggtc tgtccctgat tttgaatcga aaatctgtcc gcccgaggcg
    22501 ttggcggcgc gtttggcgtt gttgccgcgc ccgctggtgt ttaccaacgg ctgtttcgac
    22561 atcctccaca gggggcacgt tacttatctg gcgcaggcgc gttcggcagg ggcggcgttg
    22621 gtgctggcgt tgaataccga tgcttcggtg cggcgtttgg gcaagggcgg cgaccgcccg
    22681 gttaattctt tggagaaccg tgccgccgtt gccgccgcgt tggaaagtgt ggatttggtt
    22741 acttggtttg acgaggatac gccggcggcg ttgattgagg cggtcaagcc ggaggtgttg
    22801 gtcaagggcg gcgattgggt cgtggataag attgtcggtg cggcggaaac gctggcacgc
    22861 ggcggtcagg tgttttcaat tccgtttctg catcagactt cgacaacgaa gactttggca
    22921 aaaatccgtg cggcagaggg cggaaaatga cggttttgaa gccttcgcac tggcgggtgt
    22981 tggcggagct tgccgacggt ttgccgcaac acgtatcgca attggcgcgt gaggcggaca
    23041 tgaagccgca gcagctcaac ggtttttggc agcagatgcc ggcgcatata cgcgggctgt
    23101 tgcgccaaca cgacggctat tggcggctgg tgcgcccctt ggcggttttc gatgccgaag
    23161 gtttgcgcga tctgggggaa aggtcgggtt ttcagacggc attgaagcac gagtgcgcgt
    23221 ccagcaacga cgagatactg gaattggcgc ggattgcgcc ggacaaggcg cacaaaacca
    23281 tatgcgtgac ccacctgcaa agtaagggca gggggcggca ggggcggaag tggtcgcacc
    23341 gtttgggcga gtgcctgatg ttcagtttcg gctgggcgtt tgaccggccg cagtatgagt
    23401 tgggttcgct gtcgcctgtt gcggcacttg cgtgccggcg cgctttgggg tgtttgggtt
    23461 tggaaacgca aatcaagtgg ccaaacgatt tggtcgtcgg acgcgacaaa ttgggcggca
    23521 ttctgattga aacagtcagg gcgggcggta aaacggttgc cgtggtcggt atcggcatca
    23581 atttcgtgct gcccaaggaa gtggaaaacg ccgcttccgt gcagtcgctg tttcagacgg
    23641 catcgcggcg gggcaatgcc gatgccgccg tattgctgga aacattgctt gcggaactgg
    23701 gcgcggtgtt ggaacaatat gcggaagaag ggttcgcgcc atttttaaat gagtatgaaa
    23761 cggccaaccg cgaccacggc aaggcggtat tgctgttgcg cgacggcgaa accgtgtgcg
    23821 aaggcacggt taaaggcgtg gacggacgag gcgttctgca cttggaaacg gcagagggcg
    23881 aacagacggt cgtcagcggc gaaatcagcc tgcggcccga cgacagaccg gtttccgtgc
    23941 cgaagcggcg ggattcggaa cgttttttgc tgttggacgg cgggaacagc cggctcaagt
    24001 gggcgtgggt ggaaaacggc acgttcgcaa ccgtcggcag cgcgccgtac cgcgatttgt
    24061 cgcctttggg cgcggagtgg gcggaaaagg cggatggaaa tgtccgcatc gtcggttgcg
    24121 ccgtgtgcgg agaatccaaa aaggcacaag tgaaggaaca gctcgcccga aaaatcgagt
    24181 ggctgccgtc ttccgcacag gctttgggca tacgcaacca ctaccgccac cccgaagaac
    24241 acggttccga ccgttggttc aacgccttgg gcagccgccg cttcagccgc aacgcctgcg
    24301 tcgtcgtcag ttgcggcacg gcggtaacgg ttgacgcgct caccgatgac ggacattatc
    24361 tcggcggaac catcatgccc ggcttccacc tgatgaaaga atcgctcgcc gtccgaaccg
    24421 ccaacctcaa ccgccccgcc ggcaaacgtt accctttccc gaccacaacg ggcaacgccg
    24481 tcgcaagcgg catgatggac gcggtttgcg gctcgataat gatgatgcac ggccgtttga
    24541 aagaaaaaaa cggcgcgggc aagcctgtcg atgtcatcat taccggcggc ggcgcggcga
    24601 aagtcgccga agccctgccg cctgcatttt tggcggaaaa taccgtgcgc gtggcggaca
    24661 acctcgtcat ccacgggctg ctgaacctga ttgccgccga aggcggggaa tcggaacacg
    24721 cttaaaaaat atgccttttt tcaacacggg gcggcatccg tccgaatctg tccggattct
    24781 gccgcccgac agactgccgc ccgggcgggc ggggtttcaa acattgagga aattctaatg
    24841 aaatggctct tcaccctttt ggcggtactc aatattgccg tattcggcgg cacggtgggc
    24901 tataaattgg ccgttaaagc ggcgggcggc gtgccggaaa accgggccgt cgaaaacacg
    24961 ccccccgcaa cgcccgccgc cggcaatgcg gcggcatccg tggaggatac ggcggcactg
    25021 ctcaaaccgg gcgacatcct gagcgaagaa caggcggagc agttgcgctt gaaaaaagaa
    25081 gcggaacaga aaaaactgag ggagaaaaaa cagcgtgaag aaaaagcccg ccgcgaaaaa
    25141 ctcgccgccg aaaaggcgca ggcggaacgt gaaaacggcg cggcggatgc cttatgcgcc
    25201 gcgcaggcaa gcctcacgat ggacgaagac gactaccacc gcatcaaagg gcttttgggc
    25261 aaatggtcgc acgttgccag caggagcgtc gaaaaacgca ccgccaaagc caaacctgcc
    25321 gacaaaacct accgcgtcgt cctgcccgtt tccgccgatg ccgaaaatca ggcggcggag
    25381 ctgtctgcca aaggtttcaa ccccataccg tttgacggcg cattgagttt gggtgtcggc
    25441 aacagccggg aaaacgccca agccctgcaa aaccgacttg ccgatgccgg attcggcggg
    25501 gcgcatattg tcgaacactt tgccgaagcc gacaggcagg acgattcttt gagcgtgtcg
    25561 cgtatgacgg ttttgtttac gggcgtgaat gccgccgatg cggacgaaat ccgtaaaatc
    25621 acgtccctat acggcaaact gaacctcaag tcttgc
//
LOCUS       DBJGGI010000024        24367 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.024, whole genome shotgun sequence.
ACCESSION   DBJGGI010000024 DBJGGI010000000
VERSION     DBJGGI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24367
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.024"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            6..1926
                     /locus_tag="ACSIGJ_001000"
                     /pseudo
     CDS             6..1926
                     /locus_tag="ACSIGJ_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712496.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            1983..2960
                     /locus_tag="ACSIGJ_001001"
     CDS             1983..2960
                     /locus_tag="ACSIGJ_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHQ1329886.1"
                     /translation="MDMKRRDFLKMTAALAAAGVSPSLLTAGKEQFTVYGAPAMPSVT
                     IAVTALQGKLAKQADVSLKIWRSPDQLRAGVASGQFKVMMSPSNVGVNLRNQGQKVGM
                     VNILTNGITQLVCKGSAIASPQDLVGKKILVPFKNDMPDIVLQALLKKLKIDAHKVSI
                     TYAATPPEAVGLFPSKGYHAVILPEPMATASLLKGKTIGINVVHGFDLVKAWGQAFGT
                     KPLIPMAGIIANEEYFHAHKAQFDIFHQDLKNALNWILANRQNAAKIGKNYLPAPEPA
                     LVMGLDGARLTVSKGSEVKNEILKFYEILMQFNPELLGGKLPDNGFFLA"
     gene            3297..3434
                     /locus_tag="ACSIGJ_001002"
     CDS             3297..3434
                     /locus_tag="ACSIGJ_001002"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329887.1"
                     /translation="MNEDKYRENMTWDEIENVVGTLVSEERMLKSNSFLSIKNSKNRL
                     A"
     gene            3513..4283
                     /locus_tag="ACSIGJ_001003"
                     /pseudo
     CDS             3513..4283
                     /locus_tag="ACSIGJ_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776012.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            4310..5002
                     /locus_tag="ACSIGJ_001004"
     CDS             4310..5002
                     /locus_tag="ACSIGJ_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HHQ1329888.1"
                     /translation="MLCLENVRFEILRDPIVRDFSLNLQQGEVKALFGPSGCGKTTVL
                     RLIAGLETPKSGTIRNTFHKTGFLFQENRLPENLTAMQNIAIFMDNPDEGEIVALAAK
                     VGLTAGDLNKYPTELSGGMAKRVAFLRLLLCGCDLALLDEPFVGLDRDLRDILVAMLV
                     EKIERQGMACILVTHDRFEAARLSHEIMLLSAKGMNVQNVITLPTPLSERDSAFEEVV
                     VAREFQGIHYYE"
     gene            5194..5808
                     /locus_tag="ACSIGJ_001005"
     CDS             5194..5808
                     /locus_tag="ACSIGJ_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HHQ1329889.1"
                     /translation="MFSTVITAAVLYIATAVDLLVILLIFFARANTRKEYRDIYIGQY
                     LGSVILILVSLFLAFVLNYVPEKWVLGLLGLIPIYLGIKVAIYDDCEGEKRAKKELDE
                     KGLSKLVGIVALVTVASCGADNIGLFVPYFVTLDLVDLLVTLLVFLILIFVLVYTAQR
                     LANISGVGEIVEKFSRWIMAVIYIGLGLFIIIENNTIRTIISII"
     gene            6104..7199
                     /locus_tag="ACSIGJ_001006"
                     /pseudo
     CDS             6104..7199
                     /locus_tag="ACSIGJ_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
     gene            complement(7246..9435)
                     /locus_tag="ACSIGJ_001007"
     CDS             complement(7246..9435)
                     /locus_tag="ACSIGJ_001007"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HHQ1329890.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSEPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDAARILSVQTVFVEEKPLSQSWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQANAQA
                     AKLIEDWAEQGWIETTEAAKPVLRPYRGQASHSEFVLNTDQQKASDEIQTALGRFRSF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SRMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPLPDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHASAQLPQVDIL
                     NIGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIANDEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSRFGAAPMLMVRLA
                     ERERAQVFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            9575..10360
                     /locus_tag="ACSIGJ_001008"
     CDS             9575..10360
                     /locus_tag="ACSIGJ_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003756 - protein disulfide isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HHQ1329891.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAESAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRTQSGYSPMPQLEEIIRKNQQ"
     gene            complement(10459..12393)
                     /locus_tag="ACSIGJ_001009"
     CDS             complement(10459..12393)
                     /locus_tag="ACSIGJ_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HHQ1329892.1"
                     /translation="MSLIECKNINRCFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGMGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIRRLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIQEKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSMGTNTISIFPGRGFGDRRSGKIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTRVAEKGLAELLKARHGTEDFFMNNSDSIRQ
                     MVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICIIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(12459..13637)
                     /locus_tag="ACSIGJ_001010"
     CDS             complement(12459..13637)
                     /locus_tag="ACSIGJ_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HHQ1329893.1"
                     /translation="MAKMMKWSAVAAVAAAAVWGGWHYLKPEPQASYITETVRRGGIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTTQTNTLDMEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESDLGYTRITATMDGTVVAIPVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLLIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            13863..14252
                     /locus_tag="ACSIGJ_001011"
     CDS             13863..14252
                     /locus_tag="ACSIGJ_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HHQ1329894.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLIAYPSYKTYIRRARLSEVK
                     STLLVNAQNLERYYRQKGTFKNYDQTKLKQNEYFKITLIKASPDHFTLQAAPDTATNE
                     GETCTVTLNDGGTIAASGTNQSCPGFD"
     gene            complement(14346..17831)
                     /gene="smc"
                     /locus_tag="ACSIGJ_001012"
     CDS             complement(14346..17831)
                     /gene="smc"
                     /locus_tag="ACSIGJ_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HHQ1329895.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNNEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHRQNLHQRIERDKQAAQAQLQRIRQE
                     QQQIRVQLEENELQVEEKQTELAEWAMQVAEHEERLPELEEVQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARSVPNSFVPPAPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGTHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIAAELENLAPGLSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRTAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIATLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIARLEQQTLDWQERRQELALAYETEFQNDEQHIKLDELT
                     ETVHTLDEEYIAVQEKLAQIQEQGREQYARVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESAKVLNSSIGSLTRQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAIALLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCKLVKEMSVQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            18208..18876
                     /locus_tag="ACSIGJ_001013"
     CDS             18208..18876
                     /locus_tag="ACSIGJ_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329896.1"
                     /translation="MGLRQKLRGIIPNLATSIGTSAEANAPGALALGGNSEASKKFSI
                     AEGYLASSDGYGAIAIGSAAKIKQLEKGTINHIVGNDNKGLYVDADGNVTKITVRTES
                     EKDILSRYGQTYGAMALGFRSSSHNLFASSFGAFSTATAIESLAVGDSSQSTGYRSAT
                     FGSHSRALAEESLALGYETRANAYGSVALGAESVANEENTVSVGSDTLKRKIVNVADG
                     TEDL"
     gene            18848..19333
                     /locus_tag="ACSIGJ_001014"
     CDS             18848..19333
                     /locus_tag="ACSIGJ_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HHQ1329897.1"
                     /translation="MSPMARKIYDAVNVRQLNRLSKRTNRVGASAAALASLKPAQLGK
                     NDKFAFSLGFGSYKNAQAVAMGAVFKPAENVLLNVAGSFAGPDRAFGAGVSWKFGGKP
                     TPAVAAQNAAHPAKVLQLRQEVAALRARQAETDRKLHKQAEMENELQQLRRALSELKK
                     H"
     gene            19542..20264
                     /gene="lpxH"
                     /locus_tag="ACSIGJ_001015"
     CDS             19542..20264
                     /gene="lpxH"
                     /locus_tag="ACSIGJ_001015"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HHQ1329898.1"
                     /translation="MRPSYFISDLHLSEKHPELTELLLRFLRSAAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGRDFCRQAGMTLLPDY
                     SVLDLFGSNTLICHGDTLCTDDKAYLRFRRIVHCRRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPPEEC"
     gene            20533..22119
                     /locus_tag="ACSIGJ_001016"
     CDS             20533..22119
                     /locus_tag="ACSIGJ_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HHQ1329899.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYAALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(22285..22488)
                     /locus_tag="ACSIGJ_001017"
     CDS             complement(22285..22488)
                     /locus_tag="ACSIGJ_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HHQ1329900.1"
                     /translation="MDNLNPQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLGEGESG
                     SVACPYCGTRYRLDGKMPHHYYA"
     gene            22604..23101
                     /locus_tag="ACSIGJ_001018"
     CDS             22604..23101
                     /locus_tag="ACSIGJ_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HHQ1329901.1"
                     /translation="MTALTLPEDIRQQEPSVLLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGETIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHILSNRAAFA
                     HIGQA"
     gene            23183..>24367
                     /locus_tag="ACSIGJ_001019"
     CDS             23183..>24367
                     /locus_tag="ACSIGJ_001019"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HHQ1329902.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PIVKAVKEAEKRLLESETTKNYLTIDGVADYNEQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHCLDW
                     DGMIEDLNQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     STQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVK"
BASE COUNT         5489 a         6714 c         6402 g         5762 t
ORIGIN      
        1 aagaaatgaa actaaatact ctcacatggg ctttgatgac cgttttttcc gttgcgccat
       61 cttgggcaga acaaccggca aatactgaag aaatacaacc cgtcaaaacc ttctccccgc
      121 ccaaaccgat tgcaccgacc gccgcacaag gctatttccc cgaaaaccaa ttcgaccgct
      181 ccgaccgcag cgattattac tttgttaccg aaaacataga ccaagccttc cgtccgctga
      241 aggcaaacag cagtttttac ggcaaaagct tttacaactc cgttaccgcg caagcactcg
      301 gggcgaaggt atacggcgta gccaacctca atcgcaccaa ggcaaacggc tacaaagatg
      361 gcggtgggcg cgacactgat tggaaataca gccgcttcaa tcaggctttg gtactcggtt
      421 tcgtgccgtc tgaaaatcaa gaataccgcc tcacttatct gcacgacgac atcaacaacg
      481 accgccagcc gcaggtcgtc aacgacgcat tggacaccga acgccacatc tccaaactca
      541 acgtgcgttg gggcaatgcc gatttgagca atacggtcag cgcggaagca ggcgtcatca
      601 aactcaaacg ccatgccgac aattactcct tgcgaccaaa caacacgccg cagcaagtgt
      661 tcgtagaact tgaccgcaaa gtgtatgatt tctcgctcaa acacgatgcg gatttcggca
      721 aattccacaa cacggcagcc gtcagctacc gcaacgacag ccaaaacggc gaacgcaaca
      781 cccacactgc catgtgcgat ttcctcaacg gctaccgttt cgccgatgtg cacatcgacc
      841 gctggtgcat tgccgatacc ttatcttaca agtttgacga ccgacacaaa ctgggcttgg
      901 gcttaagcta cgaactcaac gaagcggaca ttcgcaaaaa tacggcacag cctgaaaacc
      961 caataaaacc cggtttcccc ttcgcctcat cacaacaaat ctggaaaacc cattacggct
     1021 acgacttcaa cggcaaagtg cgccgtcatg ctttgtcagg cgaactcaaa tacgatttca
     1081 cgccgtctga aacgcaaaaa tacagcgttt ccctcgcgca tttggaacgc atcggcgaca
     1141 acaccgaacg cttcaactcg cttgctgcca tcgtgcaaaa ccgtatgagc ggcatgctga
     1201 tgaaccaaaa tccagccgcc gccattgcag gcaatccccc tgctgaaaac tgaaaaacac
     1261 aaccgcatca ggctcaccgc cgacagccgc aacgactact acaacggcta catgaactcg
     1321 ctcgcaggcg cgggctggaa cgtgggcggc acgcttgtgg cggacaaagt caaagacctg
     1381 attatttttg accgcgcaca cggacaaagc ggcacagctt ccaaagacgg cggcatcatc
     1441 acacgcaacg tggacgcacg attgtttacc gcgcaggcct acgcgcgtta caacttcaat
     1501 ccgcattggg cggcaggcat caaagccgcc tacaactacg gacacaacga aaccgacggc
     1561 aggccgccct atcaaatccg tccgtttgaa gccgccgtcc aagccgacta caaaaactac
     1621 tttgcccacg gcagctacaa catcggcgcg gcaacacgct ttgtcgccaa acaaacgcgc
     1681 ggtgattttg atatggcaag cggtctgggc atagacaaac gcgaagccgc caaaggcttt
     1741 accgttgccg acgtgtacgc aggcgtaaac atcaaagaca aatacggctt gcgcttgggc
     1801 gtgaacaacg tattcaacaa aaaatacgtc gaatacatca gcggcgacca cgtcctcgcc
     1861 ctatccccca gcgtagtgta tgcaccgggc aggacatatt ggttgagttt gcatgcggca
     1921 ttttaaaacc atgccgtctg aaaaatacct gcattggctt attaagaatt aggagataaa
     1981 aaatggatat gaaaagacgc gatttcttaa aaatgaccgc cgcgctggca gccgcaggcg
     2041 tttcgccttc tctgcttact gccggtaaag agcaatttac cgtgtacggc gcaccggcaa
     2101 tgcccagcgt taccattgcc gtaacggcgt tgcaaggtaa actggcgaaa caggcggacg
     2161 tgtcgctgaa aatctggcgt tcgcccgacc aactgcgcgc gggcgtggca agcgggcaat
     2221 ttaaagtgat gatgagtccg agcaatgtcg gcgtaaacct gcgcaaccaa gggcagaaag
     2281 tcggcatggt gaatattttg accaacggca tcacgcagtt ggtctgcaaa ggcagcgcga
     2341 ttgcctcgcc gcaggatttg gtcggcaaaa aaatcctcgt gccgtttaaa aacgatatgc
     2401 ccgacatcgt gctgcaagcc ttgttgaaaa aactgaaaat cgacgcacac aaagtcagca
     2461 ttacctacgc tgccacaccg cccgaagcag tcggactatt tccaagtaaa ggttaccatg
     2521 ccgtcatcct gcccgaaccg atggcaaccg ccagcctact gaaaggcaaa accataggta
     2581 taaacgtcgt gcacggtttt gatttggtaa aagcatgggg gcaggcgttt ggcaccaaac
     2641 cgctgattcc gatggcaggt atcatcgcca acgaagaata tttccacgca cacaaggcgc
     2701 agttcgacat cttccatcaa gatttgaaaa acgcgctcaa ctggatattg gccaaccgcc
     2761 aaaacgcggc gaaaatcggc aaaaactacc tacccgcccc cgaacccgcc ctagtcatgg
     2821 gcttggacgg cgcacggctg acggtaagca aaggcagcga agtgaaaaac gagattttga
     2881 agttttacga aatcctgatg cagttcaacc cggaactttt gggcggcaag ctgccggata
     2941 acgggttctt cttggcttaa agggaaagag gccgtctgaa aagggtaggg tggttgtgaa
     3001 tacctgattt atcatttttc agacggccta ttaaggcatt gtcagcggcg tgggtcggat
     3061 tcttgaatcc gacatttcgg ctaaacgggg ctgtcggata caagtatccg acctactctt
     3121 ttcagacggc ctatcagata atagttcgaa cagtacacaa agagccgtcg gattgcctga
     3181 aatgttgggt catgacccaa cctacgctta ctggggaaac gggatcgaag tgtgaggtat
     3241 gtggcggggt tgtttaataa tgaaatttta taactatttg atagggtggt aatgccatga
     3301 atgaagataa atatagggaa aatatgacat gggatgaaat tgaaaatgta gtaggaactt
     3361 tagtttctga agaaagaatg cttaaatcca atagcttttt aagtattaaa aatagtaaga
     3421 ataggttggc ttaatcacaa ccataaccca acgaaaatac taaaattcca agctaaggct
     3481 atcctttcca aaccatcccc atcaaccaaa ccatgattaa aaccgacaaa atccgcaaac
     3541 cgcagcctgc gctgttttac atcatcgact acctttggag cggctttgcc ggtctgagcg
     3601 tggcgatggt ggtggtggcg ttgtgggcgt ggggcagtgc cgtgttcggc gagtttatgc
     3661 tgcctgcgcc ggtagaggtg tttcaaaaat ctttggattt attgaaacat tttcaggaaa
     3721 atgaaatcgg gatttcgctg tggcggtcgg tagtgggtat ttcggttgct ttgatagcag
     3781 gattggcggc ggggctggtg gcgggcagtt ttaagacggc gatggcgttg cttaagcctg
     3841 tgattacgat tttgttagca atgccgccga ttatttgggt ggtgatggcg ttgttttggt
     3901 tcgggttcgg caatccgagc gtgttgttta ccatcattgt gttggttgcg ccgctgacgt
     3961 ttgcgagtgc ggcggtcgga atggcgagcg tgaacaagca gcatgaggag ttgtttgacg
     4021 cttataaatt aggccgtctg aaaaaaatcc gctatctgta tatcccgcat ctgacgggct
     4081 atgtgatttc cagcgtcggc gtggcggtgg cgacgggggt gaaggcggtg atcatggcgg
     4141 aactcttagg cgcgagcaaa ggcgtgggcg cgcggattgc ggacgcgagg gcaatgctgt
     4201 agacttcgac ggtaatggct tatgtggtgt tggtcatggt atttgtgtcg ctgtttgaat
     4261 acctgattac caagcctttg taaattttgt ttatgccgtg gaggagatga tgctctgtct
     4321 tgaaaacgtg cgttttgaaa tcctccgcga ccccatcgtg cgcgatttca gtttgaacct
     4381 gcaacagggc gaagtgaaag ccttgttcgg gccgagcggc tgtggcaaga cgacggtttt
     4441 acggctgatt gcgggcttgg aaacgccgaa atcgggcacg atacgtaata ctttccacaa
     4501 aacgggtttt ctgtttcagg aaaaccgcct gccggaaaac ctgaccgcga tgcagaatat
     4561 cgcgattttt atggacaacc cagacgaagg cgaaatcgtc gcgctggcgg cgaaagtcgg
     4621 gctgactgcg ggcgatttga acaaatatcc gaccgaattg tccggcggca tggcgaaacg
     4681 ggtggcgttt ttgcgcctgc tgctgtgcgg ctgcgaccta gccttgctgg acgaaccgtt
     4741 tgtcggtttg gaccgcgatt tgcgcgatat tttggttgct atgctggtgg aaaaaatcga
     4801 gcggcagggc atggcgtgta tactggtaac gcacgaccgc ttcgaagccg cgcgcctgag
     4861 ccatgaaatt atgctgcttt ccgctaaggg catgaacgtg caaaacgtga taaccctgcc
     4921 cacgccgctg tccgaacgcg attcggcttt tgaagaagtc gtggtggcaa gggagtttca
     4981 ggggattcat tattatgagt gatgagggga tttgagtgtc tgacaaacct tacggttcgt
     5041 tgccctctcc ctaaccctct cccacgggga gagggttagc aagcctcagg cttgcctctt
     5101 tagcgaaaga tacgaatctg ttatccgaac gcgattcggt ttttgaaaaa gccgtggtgc
     5161 caagggggca ggggattcat tatgaggtgc tttatgtttt cgactgtgat tactgctgct
     5221 gttttatata ttgctacagc agtagatttg ttggtaatac tattaatatt ttttgctaga
     5281 gcaaatacta gaaaagaata tcgagatatt tatatcggac aatatttagg ttctgtaatt
     5341 ttaatattag ttagtttatt tctagctttt gttttgaatt atgttccgga aaaatgggtg
     5401 ttgggtttat taggtttaat accgatttac ttaggtatta aagttgctat ttacgacgat
     5461 tgtgagggcg aaaaaagagc taaaaaagaa ttggatgaaa aagggttgtc aaaattagtc
     5521 ggtattgttg ctttggttac agttgctagt tgtggtgcag ataatattgg actttttgtt
     5581 ccttactttg tgactttaga tcttgtcgac ttattagtta ctcttcttgt atttttaata
     5641 ttgatttttg ttttagtata tacagcacaa agattggcta atatttcagg tgttggtgaa
     5701 attgtagaga agtttagtcg ttggataatg gctgttattt atattggttt agggttattt
     5761 attattattg aaaataatac aattcgaaca ataatatcaa taatatgaat gacaagggca
     5821 tttcagtatc tgacaaacct tgcggttcgt ttccctctcc ctaaccctct cccacaggag
     5881 aggggatcag gttgctgaaa atcagagggc gaaggattgg ggatcaaatg gaaggtaaaa
     5941 cctgaaaatg ttcgggtttg acagcccgat tccctctccc cgtgggagag ggctagggag
     6001 agggcaagca agccgcaggc ttgcctcttt ggcgaaaaat acaaccctgt ccgcccaata
     6061 aatccatacc cgaaagcatc ttcatacaga atcaagccaa gcgatgaata aattttttac
     6121 ccaccctatg cgcccgtttt tcgtcggtgc ggcagtactt gccatactcg gtgcgttggt
     6181 gttttttcat caaccccggc gctatcatcc tgcaccgcca aattttcttg gaacttatgc
     6241 tgccggctgc atacggcggt tttttgacta ccgctttgtt ggaccggacg ggtttttcag
     6301 gcaacctgaa acctgccgct actttgatgg cggtgttgtt gcttgttgcg gctgttttat
     6361 tgccgttttt accgcaactt gccgcatttt tcgtcgccgc ctattggctg gtgttgctgc
     6421 tgttctgcgc ctggctgatt tggctcgacc gcaacaccga caacttcgct ctgttgatgt
     6481 tacttgccgc atttaccgtt tttcagacgg cctatgccgt cagcggcgat ttgaacttac
     6541 tgcgcgcgca agtgcatttg aatatggcgg cggtcatgtt cgtatccgtc cgcgtcagcg
     6601 tccttttggg cacggaaacc ctgaaagaat gccgtctgaa agaccccgta ttcatcccca
     6661 acgttatcta taaaaacatc gccatcaccc tgctgctgca cgccgccgcc gaactttggc
     6721 tgcccgcgca aaccgccggt tttactgcgc ttgccgtcgg cttcatcctg ctcgccaagc
     6781 tgcgcgaact gcaccatcac gaactcttac gcaaacacta cgtccgcact tattacctgc
     6841 tccagctctt tgccgccgca ggttatctgt ggacaggcgc ggcgaaactg caaaacctgc
     6901 ccgcctccgc gcccctgcac ctgattaccc tcggcggcat gacgggtggc gtgatgatgg
     6961 tgtggctgac tgccggactg tggcacagcg gctttaccaa actcgactac ccgaaactct
     7021 gccgcatcgc cgtctccatc cttttcgcct ccgccgtttc gcgcgctgtt ttaatgaacg
     7081 tgaatccgat attcttcatc accgttcccg agattctgac cgccgccgtg ttcatgcttt
     7141 acctgctgac gttcgtaccg atttttcgag cgaacgcgtt tacagacgat ccggaataaa
     7201 aatgccgtct gaaacggttt tcagacggca tcggattgcc aatgatcaag cctcctgcgg
     7261 atcgacatcc accgaccatc ggattttgcc gtcgcggttc tgctgcaaca cctgcaccca
     7321 caaactcacg gcgcggtgca aatcttgtcg ggatgttgat tcgaggaaaa cttgcgcacg
     7381 ttcgcgttcg gcaaggcgca ccatcagcat cggggcggca ccgaaccggg agacgctttc
     7441 gggcaagagc ggggcgaggg tttctttggc ggcgttgaga aattccatcg catcggcaac
     7501 acgcggcgca tcggcacgga cggccgtctg aaaaccgaaa ggcggcatgg cgaacatttg
     7561 ccgttcgttc aattcgtttt cggcaaacac ggcgtagtcc tgcgctttga cggcggcgaa
     7621 gacagggtgt tcgggcagtt gggtctgtat cagcaccttg ccgggtttgt cggcgcgccc
     7681 cgccctgccg gacacttgca tcagctcggc aaacagcctt tccggcgcac ggaaatccgc
     7741 gctgtacagg ctgccgtcgg cgttcaacac gataacgagg ttgagccgcg cgaaatcatg
     7801 gcctttggca agcatctgcg tgccgaccag aatgtcgatt tcgtcgttgg cgatgcggcg
     7861 gtacaaatct gcccagtcgt ttttgtgcgc cgtactgtcc ctatcgacgc ggacgacggc
     7921 tgccttgggc aggaaggcgc gcagggtttc ttcgacgcgc tgcgtaccgt gtccgacggc
     7981 ggtcaggtct tggttgccgc agtcgggaca tttgaacggg atgggttcgc ggtggtcgca
     8041 gtggtggcag cgcagttggc gggcgcgttg gtgcagcacc attttggcgg agcagttcgg
     8101 gcagccgaag gtatggccgc agtcgccgca aaacagcgca ggcgcgaagc cgcgccggtt
     8161 aaggtacacc agcgacatac cgcccgcttc aaagttctgt ttcaaaagct gcaaggcttg
     8221 cggtgagaag ccgttatcga gtttcagacg acctatgttg aggatgtcca cttgtggcag
     8281 ttgcgcggaa gcatgggcgc gttcggtcag ttgcagcagg cggtacgcgc cgctttgcgc
     8341 cttgtgccaa ctctccaagc tgggcgtggc actgcccaac acgacgggac agccgccctg
     8401 cttcgcccgc cacaccgcca aatcgcgggc gtggtagcgc aattcgttgt cttgtttgaa
     8461 cgagccgtcg tgttcctcat cgaccacaat cagcccgaca tcaggcaaag gcgtaaacac
     8521 cgccagccgc gtgccgatca ccagcttcgc ctgccccaac atcgcgcgca gataatcctg
     8581 cgtgcgcctg cctgccgcca tccggctgtg caacacggcg gtcggcacgt cggcaaaacg
     8641 gttttccacc cgctttaaaa gctgcggcgt gaggttgatt tcgggcaaca gaaacaacac
     8701 ctgtcgcccc tgcgccaaca ctttcgccat cgcatcgaaa tacacctcgg tcttgccgct
     8761 gccggtaatg ccgtacagca gaaacgaccg gaagcgtccc aatgccgtct gaatttcatc
     8821 ggaagccttc tgctggtcgg tatttaatac aaattcagag tgcgaagcct gcccgcggta
     8881 cggccttaaa acaggtttcg ccgcttccgt cgtttcaatc cagccctgct ccgcccaatc
     8941 ctcaatcaat ttcgccgcct gcgcgtttgc ctgtttcaac gccgccatcg tcatttcgcc
     9001 cgacagcagc gcgtcccaca aagccgcttt tttgttgaac cgagccggcg gcggcgtttg
     9061 cgccctgccc gcttcgttca aagcataaaa caacggcggc tgcggcattt ccaccgcgcg
     9121 cgtttccttc aaaccctgcg gcaatgcggc aaatacagcc tgcccagtcg gatagtggta
     9181 ataacgcgac gtaaacgcca acaaatcacg ccagctttga gacagcggct tttcttccac
     9241 aaagaccgtc tgaacgctca aaatccgcgc cgcatccata tcgggcgcaa tatccgtttc
     9301 ccacaccatc ccgacaacag ttttattgcg aaaaggcaca agcacccgcg ttcccggagg
     9361 aagcggctcg gaatgcgaat aagtcaaaag gccgtctgaa agcggcacgt ttacagcgat
     9421 gcgatggtag atcatacagg caggcaaatc atttttttgc gtattttaac agccggaagc
     9481 tattttcaga cgataaattg agcctcggcg caatgcaggc gcaacagagg tttgatacaa
     9541 tgccactttt ccaacacgct caatcatctt tgatatgaaa accaagttaa tcaaaatctt
     9601 gacccccttt accgtcctgc cgctgctggc ttgcgggcaa acgcccgttt ccaatgccaa
     9661 cgccgaatcc gccgtcaaag ccgaatccgc cggcaaatcc gttgccgctt ctttgaaagc
     9721 gcgtttggaa aaaacctatt ccgcccaaga tttgaaagtg ttgagcgtca gcgaaacacc
     9781 ggtcaaaggc atttacgaag tcgtcgtcag cggcaggcag attatctaca ccgatgccga
     9841 aggcggctat atgttcgtcg gcgaactcat caacatcgac acgcgcaaaa acctgaccga
     9901 agaacgcgcc gccgatttga acaaaatcga cttcgcctcc ctgcctttgg acaaagccat
     9961 caaagaagta cgcggcaacg gcaagctgaa agtcgccgtc ttctctgacc ccgattgtcc
    10021 gttctgcaaa cgcttggaac atgagtttga aaaaatgacc gacgtgacgg tttacagctt
    10081 tatgatgccc attgccggcc tgcacccaga tgccgcgcgc aaggcgcaaa tcttatggtg
    10141 tcagcccgac cgtgccaaag cgtggacgga ttggatgcgt aaaggcaaat tcccggtcgg
    10201 cggcagcatc tgcgacaatc ccgtcgcgga aaccacttcc ttgggcgaac agttcggctt
    10261 caacggcacg ccgaccctcg tcttccccaa cgggcgcacc caaagcggtt acagcccgat
    10321 gccccaactg gaggaaatca tccgcaaaaa ccagcagtaa acccgcaatg caaaaggctt
    10381 ggaaccatgt tccaagcctt tttcaaatgc cgtctgaagc ctgtcgcggc agcttcagac
    10441 ggcatctttg ttcaaacctc aatcctgcgc caatgcgtct atcggattga gtttggctgc
    10501 cttattggcg ggcataaagc cgaacgcgat gccgattccg gtcgaacagg cgaccgcccc
    10561 gataacggat gccgccgaaa tgtccatcgg gaaatcggtt acaaaatgat tgaacacgag
    10621 gctgacggcg gcggacaaac ctacgccgac caagcctccg atgatgcaga ttaacaccgc
    10681 ctcaatcaaa aactgctgca aaatattgcc gcgccgcgcg ccgattgcca tccgtatgcc
    10741 gatttctttg gtgcgctcgg taacggacac cagcatgatg ttcatcacgc cgatgccgcc
    10801 gactaccaat gaaatcaggg cgatggagga aatcagcagc ttcatcgtac cggtggtgct
    10861 ttcgaccatc tgcctgatgc tgtcgctgtt gttcataaag aagtcttccg tgccgtgccg
    10921 tgctttgagc agctcggcca gccctttttc ggcaacccgg gtattggcat tgtctttgat
    10981 tttgacggtg atggagttgg tgtggctctc gcctgtgatt tggtgcatca ccgtcgtata
    11041 gggcgaccaa agcatcagca cgtcggaatt gccgaaagcg ttttcgtctt ttttcatcac
    11101 gccgatgacg gtcaaggggc gtttcctgaa caaaatggtt ttacccaacg gatccgagtc
    11161 cgcaaagagt ttgtctttga cattttggtc gatgacgacg acttgcgcgt cttctttcac
    11221 atcgttctca tcaaacagcc gccccgtttc cagcttcagc ccgcgcacgt cgaaatattg
    11281 ttcgcccacg ccgtacaaag aagcggtcag gtcggtattg cggtaggtca gcgtgccgcc
    11341 gctcgaagtc atgggcgtgg cggaggcaac gtagctttgt ttggcgatga tttttgcgtc
    11401 gtctatggtc agggttttga ttttgccgct gcgcctgtcg ccgaagccgc gcccggggaa
    11461 gatgctgatg gtgttcgtcc ccatcgaact gatgtcttcg aggatttttt tctgcgaacc
    11521 gttgcccaat gcgacgacgg agacaaccga agcgataccg atgatgatgc cgagcatggt
    11581 cagaagcgaa cgcattttgt gcgccaatac tgcttgtacc gacattctga aggcttcgac
    11641 aaactggtcg taataaaacg accacgaggc tttttcctga atcctcccga cattgcttgc
    11701 ggggatttcg ggatttttcg aggtgtcgga aatgatttcg ccgtcccgga tttcgatgac
    11761 gcggttggca ttggcggcaa tgccggggtc gtgcgtgacc ataatgacgg tatgcccggc
    11821 ttcgtgcagc ctgcggatga tttccatcac atttttgccg ctggcggtat cgagtgcgcc
    11881 ggtcggctcg tcggcgaaga tgatttctcc gccgttcatc agggcgcggg cgatggagac
    11941 gcgctgctgc tgtccgcccg agagttcgcc gggcttgttg ccctctttac ttgccaaacc
    12001 caaatcctgc aagagtttgt ccgcccgcgc ggaacgctct ttgccgccca tacccatata
    12061 cacggctggc agcgcgacgt tgtcccttgc ggtcagcgag cttaagaggt tgtagcgttg
    12121 gaagatgaaa ccgaagcgtt cgcggcgcaa tgccgccagt tcgtcaggct gcattttggc
    12181 agtttcgatg ccgtcgattc ggtacgaacc ggaaccggcg gtatccaaac aaccgagtat
    12241 gttcatcagc gtggacttgc ccgaaccgga ctgcccgatg atggcgacaa aatcaccctt
    12301 ctctatcgac aggctgatgt ctttcaaaat atggacgcgg ttctcgccgc tgccgaagca
    12361 gcggttgatg tttttacatt cgatcaagct cataatattc cgtaaatcaa taacaaatgc
    12421 cgtctgaaac cgtttccgtg ttcagacggc atattcgttt atcggcgcgg cgggccgcct
    12481 agggcgcgtt cgccgctttc ctgctgctcg gcggcggtta tttcggagat gaccactttg
    12541 tccccctctt tcaacccgct tttcacttcg gtattcatac tgtctctcat accggtccgg
    12601 atttcgcgtt ccgccgcctt gccgtccgca cccaacacgc gtacgaacgc cttgccgccg
    12661 cgatttttca cggtcagcga cggaataagc aacacatttt tcacaccgtc gatttcaacc
    12721 gtattctgcg tcgtcatccc cgtggcgagt ttgccgtccg gattcggcac aaacgaacgg
    12781 gcataataat agaccgcatt ggaagccgta tccgtactgc tgttgtagcc gcccgacgac
    12841 atcgtggtca gcccggggtc gacgctgtcg agcttcgcct taatcggcgt atccggttcg
    12901 gacaaaatcg taaacgaaat atcctgcccc gccttcacct tggtaatatc gccctcggca
    12961 atctgcattt tgttcaacat catatccaga ttcgccaatt ggacaatcgt cggcgtagac
    13021 tgcgccgcgt tcacagtctg cccctcttcc acgggaatcg ccaccaccgt gccgtccatc
    13081 gtcgcggtaa tgcgcgtgta gcccaaatcc gactcggcgg tattgatgga aattttgctc
    13141 tgtctgatta aagccttcaa ctcggcaaca ttggctttgg cggcggcaag cgcatcctgc
    13201 gcgctttcca aatcttcttt agaggtcgca tcatccttcc acaacgccgc ctgacgctta
    13261 tatttcttct ccgcgctgcc caatgcaatc tgtgccgaca ccagcttcgc ctgatacgtt
    13321 tccaatttgg atttttccat atcgagcgtg ttggtctgcg tggtcgaatt gatttccgca
    13381 atcaaatcgc cctttttgac ctgttgcccg agtttgacat aaagcttttt aatctgcccc
    13441 gaagcctgcg cgccgaccga taccaggttg gacggcgaaa tctcgcccgt cgcggaaacc
    13501 gtccggctga tgccgccgcg cctgaccgtt tccgtaatat aagaagcctg cggctcgggc
    13561 ttcagataat gccatccgcc ccaaaccgct gccgccgcga ctgccgcaac agccgaccat
    13621 ttcatcattt tcgccatatt ttcaattctc tgtatgcgtc aatcccataa accggaagcg
    13681 gattctaccg caagtccctg ccccatccaa ggcggcaaac cgccgacaat aaaaataacc
    13741 atttgtttta aatggcttat acaatctgct ttcccgaaaa tttcaatccg tttacatatt
    13801 ccgccatttc cgatacggcg ttataatgcc gcccacaaac aaccaaatgg aaacacattg
    13861 ctatgaaaaa cgttcaaaaa ggctttacgc tgctcgagct gatgattgcc gtcgccatcc
    13921 tcggcatcct gacgctcatc gcctaccctt cctataaaac ctacatccgg cgcgcccgcc
    13981 tgtcggaagt caaatccacc ctgctggtga acgcgcaaaa cttggagcgt tactaccgcc
    14041 aaaaagggac gtttaaaaac tacgaccaaa ccaaactgaa acaaaacgaa tatttcaaga
    14101 ttaccttaat caaagccagc cccgaccact tcacccttca ggccgctccc gatacggcga
    14161 ccaacgaagg cgaaacctgc accgtcacgc tcaacgacgg cggtaccatt gccgcttccg
    14221 gtacaaacca atcctgcccc ggcttcgact gattggctgt tgcagtcggc attgtgccta
    14281 aaccatctat aaaaatgccg tctgaacgct gttcagacgg catttttgac atcccgatac
    14341 gcctttcaaa cggcttccgc catttccaac gcctgcttga tgtctacggc gacgacgcgc
    14401 gacacgcctt tttcctgcat ggttacgccg accagctgct ccgccatttc catggtcagg
    14461 cggttgtggg agatgtagag gaactgggtt tgcaccgaca tttctttgac cagcttgcag
    14521 aaacgcgagg tgttggcatc gtccagcggg gcatcgactt cgtccagaag gcagaagggg
    14581 gcggggttga ggctgaacag ggcgaacacg aggctcatgg cggtgagggc tttttcgccg
    14641 ccggagagga ggtggatggt gctgtttttc ttgccgggcg gacgcgccat gatggacacg
    14701 ccggcggtca ggaggtcgtc gcctatcatt ttgagggtgg cttcgccgcc gccgaacaga
    14761 gtcgggaaga aggtttggac tttgccgttg acggcgtcga aagtttcttt gaaacgcgct
    14821 ttggttttgt cgtcgatttg ggcgatggct tcttccaaaa gcgcgatggc ggcctgcacg
    14881 tcttcgcttt ggctgcggta gtagccgtcg cgttcgcgcg cttcttcgag ttcttgcagg
    14941 gcggcgaggt tgacggcgcc gagggcttcg atttgccggg taaggctgcc gatgctgctg
    15001 ttcaatactt tcgccgattc tttcgccaac gcttcgagcg cgtccagatc ggcagcgcgt
    15061 tcggtcaggt tttgatggta gcgtttggcg ttgatcaggg cctcctgctg ctgcaacagg
    15121 gcggtttggg tggcggcctg aagctgcggc agcttggttt gcagggtttg tacgcgggcg
    15181 tattgctccc tgccctgctc ctgaatctgc gcgagttttt cctgcacggc gatgtattct
    15241 tcgtccagcg tgtgtacggt ttcggtcaac tcgtcgagct tgatgtgctg ctcgtcgttt
    15301 tggaactcgg tttcataggc gagggcaagc tcttgccggc gttcctgcca gtccagggtt
    15361 tgctgttcga gccgggcgat ttgctgccgg tagttttgtt tttgctggtt gagtttgtgg
    15421 acggcgactt cggcaagccc gtattggcgg ttggcttcca acagggcaag ctgcgcctgt
    15481 ttcagacggc cttgctgctc ttggcggctg tgcgcggtgg tttgctgctg gtgttcgagt
    15541 tcggcggcgg cttcctgcaa agtagcgatg tcgtctgaaa gcccgtcgga cgtgtgttgc
    15601 aacacggtct gctcttccgc caactgtgcc agttcgcgct cgatgtgttc gcggcggatt
    15661 tgcccttggt tggtgcgcgc cagaagttcg gcggtgcgtt gctgcgcttg actgtattgg
    15721 cgcgtgtgtt gctgttgctg ctgcatcaga tttttgtgct gcacttcgga cgagcgcacg
    15781 gcagcctccg cctgtttgaa cgcggcttcg gcggcggaaa gtccgggggc gaggttttcc
    15841 agttcggccg cgatgccgtc gaggcgtgct ttttgggcaa tcaggctttc ctgcgcgggc
    15901 ttggcataga gcaggacgct gactttatcg acctgatggc cttcgggtgt gagccagatt
    15961 tgatgcgtgc ccaaatcgct ttgatgcgcg agggcgtagc tcaaatcggg cgcgcacaat
    16021 acgccgtcga gccagtggtg caatgccgtc tgaaacggcg gctgcgcttg gatttggttc
    16081 agcaatgcct gtacgggcag ggattttttg atgccgccgg agaggccgtc tgaaagccat
    16141 gccgcctgcc cctgcggcaa aggtgcgggc ggtacgaaac tattcggcac gctacgggcg
    16201 tgcaggcgtt cggcaaggat gacggacagg gcgtgctgcc actcggcggg cgcggtgatg
    16261 tgttgccaca gttgcggcgc ggcggcgtgg tcggttgcct gccaaaagtc ggcggcttcc
    16321 tgctgttgcg acaggatttg cgacaacgcc tgctgctgcg cctgcaaggt aatgtgttgc
    16381 tgcttcaggc tttggaagcg gtttgaggcc gtctgaaacg cctcgcgggc ggcgtgcaag
    16441 gcttcttcgg cggcaatgat ttgttcttcg taatgctctt gccggctttg caacaaggcg
    16501 gcggcttcct gagcggcggc ggtttcggcc tcgtcgggca ggttcagggc ttggttttcc
    16561 tgcttcagac ggcctttgcg ctcttcgtgc ttggcaacgg tttgttcggc atgggcaagc
    16621 tgctgctgct tcaacgccag ttcgcggcgg atgcggtttg cctcgtcctg ctgggtttgg
    16681 aaggcagcgt tgagcgtggc ttggacttct tccaattcgg gcagacgctc ttcgtgttcg
    16741 gcaacctgca tcgcccattc cgccaattcg gtttgctttt cttcgacctg caactcgttt
    16801 tcttcaagct gcacgcggat ttgctgctgc tcctgccgga tgcgttgcaa ctgcgcctgc
    16861 gccgcctgct tgtcgcgttc gatgcgttgg tgcaggtttt gccggtggcg gatttgttct
    16921 tccaaacggg caatctgctc gcgcaacacg ccgcgtttgt tgctcaattc gtgtactgcc
    16981 tgctgctgcg actgttcggc agtctgcaag gcgtgtactt cgttatttaa cgcctgaacc
    17041 tgtgcggcgg tttcatcctg ctgcgcctgc aaagattgat gctgcgcggt cgccttgtcg
    17101 gcggcggcaa gcgattgctg ccattgggcg taatcgagca aatcctgttg ttgattcaac
    17161 tgcgcggtca gggatttgta gcgttcggcg gtttccgcct gtttttccag cttttccacc
    17221 tgacgcgcca actcgttctg caaatcgccc aaacgctgca aatgctcgcg cgtgtctttc
    17281 agacggcctt ccgtctcctt gcggcgttcc ttatatttgg acacgcccgc cgcctcctcg
    17341 atataggcgc gcaactcctc cggccgcgct tcgatgatgc gcgaaatcat cccctgctca
    17401 atgacggcat aaccgcgcgc gcccacgccc gtacccaaga acaaatcggt aatatcgcgg
    17461 cggcgcacgg tctgattgtt gatgaaataa gtcgattccc cctgacgcgt cagctgccgc
    17521 ttgatgctca cctcggcata ctgcccccac gcgccctgca aactgtggtc gctgttgtca
    17581 aataccagct ccaccgaagc cctcggcgca ggacggcgcg tcgccgcacc gttaaaaatc
    17641 acgtcctgca tactctcgcc gcgaagctgc ttcgccgaag cctcgcccaa cacccagcgc
    17701 accgcgtcaa tcacattcga cttgccgcag ccgttcggcc cgataaccgc gacaagctgc
    17761 cccggaacat gaatcgtggt cgggtcggta aaagatttga agccggagag tttgatgtgg
    17821 gtcaggcgca taatggtcgg aaaaaataaa aaagaagcgt attttaacgg aaatcccgcc
    17881 gcaccacccc atatcttgcc ggcaaaacct taccgcatcc cgccccggtg cggcaggatt
    17941 ttgcacatcc cggccatccg tattaaaatc tttgcgtttc ccacactttc ctgacaaata
    18001 tacggaggag gattccatga aaacaaaaac agtcaagcaa gcaagcaagc aagcaagcaa
    18061 gcaaaatagt ctaacccgcc tttctttaat cgctctactt aatttccact tctttttatc
    18121 ctgttcgatc ttttgcggct gcacctcaag aaataaacta ttcgttatca ggcggtaaag
    18181 tttacgcaga taagttcagt atcgccattg gggttgaggc aaaagctcag ggggataata
    18241 ccaaacttag caacctcaat cggcacatct gccgaagcaa atgcacccgg ggcattggct
    18301 ttagggggaa attctgaagc atcgaaaaaa tttagtatag cagaaggcta tctggcatct
    18361 agtgatggct atggtgcaat agcgattggt tctgccgcca agataaagca attggaaaaa
    18421 ggcacaataa atcatattgt gggaaacgat aataaaggtc tttatgtcga tgcagacggc
    18481 aatgtaacaa agataaccgt aaggactgag tcggaaaaag atattctctc aagatacggt
    18541 caaacttacg gtgcgatggc actagggttt agatcttctt cacataatct ttttgccagt
    18601 tcatttggag cgttttctac agccacagct attgaaagct tggcagtcgg cgacagcagc
    18661 caatcaacgg gctaccgcag tgctactttt ggcagtcaca gcagggcttt ggcagaagaa
    18721 agtttggcat taggttatga aactcgggca aatgcttatg gttccgttgc tttaggtgca
    18781 gaatctgtgg cgaatgaaga aaataccgta tcagtgggtt ccgatacatt gaaacggaaa
    18841 atcgtcaatg tcgccgatgg cacggaagat ttatgatgca gtaaatgtcc gccagttgaa
    18901 ccgcttaagc aaacgtacaa accgcgtcgg cgcaagtgct gcggcgttgg cttcgttaaa
    18961 acctgcacaa ttaggcaaaa acgacaaatt cgccttttct ttgggctttg gaagctataa
    19021 aaatgcccaa gcggtggcaa tgggggcggt gtttaagccc gctgaaaatg tgttgcttaa
    19081 tgtggcgggc agttttgccg gaccggaccg ggctttcggc gcgggggttt cttggaaatt
    19141 cggcggcaaa ccgacacctg cggttgccgc acaaaacgcg gcgcatcctg caaaagtttt
    19201 gcaattgcgg caggaagtgg cggcgttgcg cgcccggcag gcagaaaccg accgcaaact
    19261 gcacaaacag gccgaaatgg aaaacgagct gcaacagttg cgccgcgcgt tgtccgaatt
    19321 gaaaaaacat tgaagcaata aacgtaaggc gggacggcgg ctctgccttt ttccgatgcc
    19381 gcacccgcgc gatgccgtct gaagccttca gacggcattt ttaacgcgcc cgaaccccgg
    19441 ttccgccaat gcccgatgct tggcgcggca tttgccgtat tgccccgccg ccgggcgcgt
    19501 tataatccgc cacgacatcc gcccgatttg gaaacctgcc tatgcgaccc tcctatttca
    19561 tttccgacct gcatttgagc gaaaagcacc ccgaactgac cgaactgctg ctgcgttttt
    19621 tacgttctgc cgccgccggg caggcgcggg cggtttatat tttgggcgat ttgtttgatt
    19681 tttgggtggg cgatgacgaa gtttccgagt tgaatacttc ggttgcgcgg gaaatcagga
    19741 aattgtccga caaaggcgtt gccgtgttct ttgtcagggg caaccgcgat ttcctaatcg
    19801 gtcgggattt ttgccggcag gcgggtatga cgctgctgcc ggattactcg gttttggact
    19861 tgttcggcag caacaccctg atttgccacg gcgacacatt gtgcaccgac gacaaggcat
    19921 acctgcgttt ccgccgcatc gtgcattgca ggcggctgca aaaactgttc ctgatgctgc
    19981 ccctgaagtg gcgcacgcgc cttgccgcca aaatcaggcg tgtcagcaaa atggaaaaac
    20041 aggtcaagcc cgccgatatt atggatgtca atgccgcctt taccgcgcgg caggttcgcg
    20101 cctttaacgc ggaaaggctg atacacggac acacccaccg cgagcatatc catcacgaaa
    20161 acggctttac ccgcatcgtt ttgggcgact ggcataacga ctatgcttca atcctccgcg
    20221 tggacgggga cggcgcggta ttcgtgccgc cggaagaatg ctgaaaatgc cgtctgaagc
    20281 ctttcgggcg gctttttcta cacgcccctt tcccacactc cctccgccgc atcatcacca
    20341 tccttaaccc gcattaatag ccattatcga taaaatgctt gaccgattac ataagattac
    20401 gtaaagtgtg caaacgcata ataattggtc ttaccaattt atcttttaac caaaatatcg
    20461 ggtgtgcggg atttgaacgt ctttccaaac ctcgacgcgc cttatgatgc aaatgcctaa
    20521 aaaggattcg ttatggcact tttcctcagc atattcccca tcgtcctgct gatttggctg
    20581 atggtgaaaa aaaacagtat gccttcctac gtcgcactgc cgattaccgc agtgctgatt
    20641 tacgccatca aacttttcta cttcggcgat gcgggaatgc tgctcaacgc caccgccgct
    20701 tccggcctcg tcaaaacgct cacgccgatt accgtgattt tcggcgcgat tatgttcaac
    20761 cgtatgatgg aaaccacggg ctgcatcgat gtcatccgca aatggctggc gaccatcagc
    20821 ccgaaccccg tagcgcaact gatgattatc ggctgggctt ttgcctttat gattgaaggc
    20881 gcatccggtt tcggcacgcc tgccgcgatt gccgcgccga ttctgatgag cttgggcttc
    20941 aatccgttga aagtggcgat tttcactttg gtgatgaatt ccgtgccggt atcgttcggc
    21001 gcggtcggta cgccgacctg gttcggtttc gcaccgctga acctgagtgc cgaagacatc
    21061 ctcgccatcg gcaggcagac cggcgtgatg cacttcttcg caggtttcgt catccctgtc
    21121 atcggtttgg gcttcatcgt gccttggtct gaaatccgca aaaacttggg cttcgtcgcc
    21181 attgccgtct tctcctgcac cattccttat gccgcattgg cgatggtcaa cgaagaattc
    21241 ccgtcgctcg tcgccggcgc aatcggcctg atggtgtccg tattcgccgc caaccaaggc
    21301 tggggcttga gcaaagacca cgccaaagac ccgaatgccg aaaaagtgcc gttcgcccaa
    21361 gtcgccaaag cactcgcccc tttgggtatg ctgatcggca tgctggtggt tacccgcatc
    21421 aaacagctcg gaatcaaagg cattttgacc agcaaagaag aatggttcag cttccaactg
    21481 ccgtttgatt tgtccaaaat taccgtcagc gactccctga cgattacctt cggcaatatt
    21541 ttcggacaag atgtcagcgc gtcttaccaa acgctgtacg tcccggcttg gattccgttc
    21601 gtgctgaccg tttggatttg catcctgctg tataaaacca aattcaaaga tgcctggacg
    21661 atttatgccg taaccttcaa tcaaaccaaa aaaccgctgc ttgccctgat gggtgcgctg
    21721 attatggttc agctgatgct ggtcggcggc gacaattcga tggtgaaaat catcggtaag
    21781 gaatttgccg caatggcggg cgaacactgg gtttattttt cgccgtatct gggcgcgatc
    21841 ggtgcgttct tctccggctc caacaccgtg tccaacctga ccttcggtcc gattcagcag
    21901 caaatcgccc ttgataccgg cctgtccgtg accctgattc tggcgttgca gtccgtcggc
    21961 ggcgcgatgg gcaatatggt gtgcctcaac aacatcatcg ccgtatgtac cgtattggac
    22021 gtgaaaaatt ccgaaggtgc gattatcaag aaaaccgtta tcccgatggc gatttacggc
    22081 gtgattgccg tcgtcgcggc aatgattttc ttcctctagg cgaaacgccg ccatccatag
    22141 cggcagcacg cggggaacgg aaaccgccgc tcgtccgggc ggcgcgtcgg gcagaatccc
    22201 ggtgccggaa cagcccgccc tgccaaacaa atgccgtctg aaaccggaaa aggcttcaga
    22261 cggcattttt atcgccgttt gcattcaggc gtagtaatga tgcggcatct tgccgtcaag
    22321 gcggtagcgc gtgccgcaat acgggcaggc aacgctgccc gattctcctt cgcccaaagg
    22381 taaaaacacc ctcggatgcc cgttccactg ctcgttgtcc ggtcccgagc aatacagcgg
    22441 cagattttcc ggcaacacgg aaatttcctg cggattcaga ttgtccattt gattttcctt
    22501 tgcgtggttg gcgtgcgccc attttacgcc atcggcaggc taaaggatat tttcggcgca
    22561 aagccgcaat ccgctataat ccccactttt cagacggcat accatgactg cgcttaccct
    22621 tcctgaagac atccgccaac aagaaccatc cgttctgctc tataccctcg tttccgccta
    22681 cctcgaacac accgcccaaa ccggcgacga atccctctcc tgcctgtccg acgaccagca
    22741 cacgctgacc gcattctgct acctcgacag ccaagtcgaa gaaggcggct tcgtacagct
    22801 tatcgcctcc ggctacggcg aatacatttt ccgcaacccg cttgccgaca gcctgcgccg
    22861 ctggaaaatc aaagccgtgc cgaaagtctt ggacaaagcc aaagccctct acgaacaaca
    22921 cggcgaaacc atcgaaacgc tcgccgacgg aggcgcagac atcgaatccc tgcgcaaaca
    22981 gttccccgac tttgaagaat gggacggcgc atactacgaa gccgccgaac aagacctgcc
    23041 cctgcttgca gaacacatcc tgtcaaaccg ggcggcattc gcccatatcg ggcaggcgtg
    23101 attgcgtctg tttccagccc gtgtaaaaca gcgtaaaatc ggcaaacccg aattatcttc
    23161 cgtccccatc aaggagcaag ctatgttctt caagcacatc gaagccgccc ccgccgatcc
    23221 gattctcggt ttgggcgaag cgttcaaagc cgaaacccgc cccgaaaaag tcaacctcgg
    23281 catcggcgtg tacaaagacg catccggcgc gacacccatt gtcaaagccg tcaaagaggc
    23341 tgaaaaacgc ctgttggaaa gcgaaaccac caaaaattac ctgaccatcg acggcgttgc
    23401 cgactacaac gagcaaaccc aaatcctgct gttcggcaaa gaccacgaaa tcatcgccag
    23461 ccgccgcgcc aaaacagcgc aaagccttgg cggtacaggc gcattgcgta ttgcggccga
    23521 atttgccaaa cgccagttga acgcgcaaac catctggatt tccaatccga cctggccaaa
    23581 ccacaacgcc attgccaaag cggtcggtat ccaagacaaa ccttatcgct actatgatgc
    23641 cgccaaacac tgcttggatt gggacggcat gattgaagat ttgaaccaag cgcaaaaagg
    23701 cgacatcgtc ctgctgcacg gctgctgcca caaccctacc ggtatcgacc ctacgcccga
    23761 acaatgggaa actttagcaa aactttctgc cgaaaaaggc tggctgccat tgtttgactt
    23821 tgcctaccaa ggcttcggca acggtttgga agaagatgcc tatggcttgc gcgtgttctt
    23881 gaaacacaat acagaattgc tgattgccag ctcttattcc aaaaacttcg gcatgtacaa
    23941 cgagcgtgtc ggtgcattca ctttggtggc cgaagatgaa gaaacagcag cccgcgccca
    24001 cagccaagtc aaaaccatca tccgtacctt gtattccaac ccggcttcac atggtgcgaa
    24061 caccattgca ctggtgttga aaaatgatga tttgaaagca caatggattg ccgaactcga
    24121 tgaaatgcgc ggccgcatca aagccatgcg ccaaaaattt gtcgagttgc tcaaggccaa
    24181 aggttcaacc caagactttg attttattat tgaacaaaac ggtatgttct ctttcagcgg
    24241 cttgactccc gaacaagttg accgtttgaa aaacgagttt gccatttatg ccgtccgttc
    24301 cggccgcatc aacgtcgccg gcattaccga cgacaacatc gattatctgt gtgaaagcat
    24361 cgtgaaa
//
LOCUS       DBJGGI010000025        23930 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.025, whole genome shotgun sequence.
ACCESSION   DBJGGI010000025 DBJGGI010000000
VERSION     DBJGGI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23930
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.025"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(<1..149)
                     /locus_tag="ACSIGJ_001020"
     CDS             complement(<1..149)
                     /locus_tag="ACSIGJ_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HHQ1329903.1"
                     /translation="MTELPWIAEARRHIGLKEIPGAKHNPTIVQWLKETGGFPGAAKS
                     WYFED"
     gene            complement(452..628)
                     /locus_tag="ACSIGJ_001021"
                     /pseudo
     CDS             complement(452..628)
                     /locus_tag="ACSIGJ_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(854..2071)
                     /locus_tag="ACSIGJ_001022"
     CDS             complement(854..2071)
                     /locus_tag="ACSIGJ_001022"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HHQ1329904.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLATGEQVTIGLLAMALKDIGVDAKSYTGWQVSL
                     KTDTAHTKARIESIDDEKMRADLAAGKVVIVAGFQGISSEGNISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRILSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERKDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFDLG"
     gene            complement(2273..3001)
                     /gene="rph"
                     /locus_tag="ACSIGJ_001023"
     CDS             complement(2273..3001)
                     /gene="rph"
                     /locus_tag="ACSIGJ_001023"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HHQ1329905.1"
                     /translation="MPDYIRTSRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SVDENAPPFLHGKNQGWVTAEYGMLPASTALRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPILE
                     AVAAVSAGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEGAPFSLDELG
                     KLVALAQKGIGELLRYQQNALSVA"
     gene            complement(3083..3547)
                     /locus_tag="ACSIGJ_001024"
     CDS             complement(3083..3547)
                     /locus_tag="ACSIGJ_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HHQ1329906.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVGNTVKHLLVANKGAREMAQDL
                     VDYADENGAGLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3764..4540
                     /locus_tag="ACSIGJ_001025"
     CDS             3764..4540
                     /locus_tag="ACSIGJ_001025"
                     /EC_number="6.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_function: GO:0061503 - tRNA threonylcarbamoyladenosine
                     dehydratase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HHQ1329907.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALTHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKEKMRVPCVYSTENIMPPQSGAAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIAGKK"
     gene            4544..4675
                     /locus_tag="ACSIGJ_001026"
     CDS             4544..4675
                     /locus_tag="ACSIGJ_001026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329908.1"
                     /translation="MPSETGFRRHLNKRGNQAVGSEKPTSARLKPYSQSTPFQNICI"
     gene            4785..5639
                     /locus_tag="ACSIGJ_001027"
     CDS             4785..5639
                     /locus_tag="ACSIGJ_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HHQ1329909.1"
                     /translation="MPPIPALSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKIVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVEITGQYLHYIAQMEAYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAAETKLPEVYIADLMQAWLGILSRAKHTRSHEVIQETLSVRAQMTAILRRLNEHGIC
                     RFHDLFNPEQGAAYVIVNFIALLELAKEGLVGIVQEDGFGEIRISLNHEGAHSDGIFG
                     TRGGRDVF"
     gene            5680..6888
                     /gene="pncB"
                     /locus_tag="ACSIGJ_001028"
     CDS             5680..6888
                     /gene="pncB"
                     /locus_tag="ACSIGJ_001028"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HHQ1329910.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNVSTVY
                     PLADIREDLEAELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEAGTD
                     DKGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVYLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKG
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFGIPEPRTP"
     gene            6980..8698
                     /gene="argS"
                     /locus_tag="ACSIGJ_001029"
     CDS             6980..8698
                     /gene="argS"
                     /locus_tag="ACSIGJ_001029"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HHQ1329911.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRHEFLAQNIHAALNDA
                     RFGVAKTAQPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLEFTGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVADDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRIGRLKAGRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENAKAEFIGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATAPTVLTEPLEKQLAAELLKFENVLQSVAD
                     TAYPHYLAAYLYQAATLFSRFYEACPILKAEGASRNSRLQLAKLTGNTLKQGLDLLGI
                     DVLDVM"
     gene            9270..10688
                     /locus_tag="ACSIGJ_001030"
     CDS             9270..10688
                     /locus_tag="ACSIGJ_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HHQ1329912.1"
                     /translation="MNIHALLSEQWTLPPFLPKRLLLSLLILLAPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWPAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAVKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAAMRL
                     SEPKSQKILFIVAESWGLPGNPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQKIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIRRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
     gene            complement(11141..11887)
                     /locus_tag="ACSIGJ_001031"
     CDS             complement(11141..11887)
                     /locus_tag="ACSIGJ_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HHQ1329913.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAACSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTAALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(11871..12050)
                     /locus_tag="ACSIGJ_001032"
     CDS             complement(11871..12050)
                     /locus_tag="ACSIGJ_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329914.1"
                     /translation="MNGKCRLKTARPAFLRRRATAVCRDKKVQAAVFTVKWRIILQRR
                     QFRRFLIRTVYGFSF"
     gene            complement(12066..12737)
                     /locus_tag="ACSIGJ_001033"
     CDS             complement(12066..12737)
                     /locus_tag="ACSIGJ_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HHQ1329915.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYDSEAVRTAVAVLDVLGTAG
                     DVGSDAPVRRRASAKSGHSYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFETDRYQREVAQVSAGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDSVGE
                     WLGIW"
     gene            complement(12806..13477)
                     /gene="rpiA"
                     /locus_tag="ACSIGJ_001034"
     CDS             complement(12806..13477)
                     /gene="rpiA"
                     /locus_tag="ACSIGJ_001034"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HHQ1329916.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSGELLARYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEAVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(13554..14036)
                     /gene="ispF"
                     /locus_tag="ACSIGJ_001035"
     CDS             complement(13554..14036)
                     /gene="ispF"
                     /locus_tag="ACSIGJ_001035"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HHQ1329917.1"
                     /translation="MTNIRIGQGYDVHQLIEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(14068..14757)
                     /gene="ispD"
                     /locus_tag="ACSIGJ_001036"
     CDS             complement(14068..14757)
                     /gene="ispD"
                     /locus_tag="ACSIGJ_001036"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HHQ1329918.1"
                     /translation="MKRKNIALIPAAGIGVRFGADKPKQYVEIGSKTVLEHVLGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAVPVADTLKRAESGQISATVDRSG
                     LWQAQTPQLFQAGLLHRALAAENLDGITDEASAVEKLGVRPLLIQGDARNLKLTQPQD
                     AYIVRLLLNAV"
     gene            complement(14754..15488)
                     /gene="dnaQ"
                     /locus_tag="ACSIGJ_001037"
     CDS             complement(14754..15488)
                     /gene="dnaQ"
                     /locus_tag="ACSIGJ_001037"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HHQ1329919.1"
                     /translation="MMTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEEKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGETEEEEPTAKPVASAEMKLGAKLKVIKADEAELAAHE
                     EYLDGLGEACIWRKEAVPSENGGENA"
     gene            complement(15644..16954)
                     /locus_tag="ACSIGJ_001038"
     CDS             complement(15644..16954)
                     /locus_tag="ACSIGJ_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHQ1329920.1"
                     /translation="MDAKKGGLGLVKSRRFGPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGVLPAGQMLNLGALLFILPYFLFSALSGQLGNKFDKAVLARWVKVLEIIIMAV
                     AAYGFYIRSAPLLLLCLFCMGAQSTLFGPLKYAILPDYLDDNELMMGNSLIESGTFVA
                     ILFGQILGTAVAGAPPYIVGILVLLVAVGGTAGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHNPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFGRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYSVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLAVFLIKRERRFLGAAAIRKKP"
     gene            complement(16944..17129)
                     /gene="ccoS"
                     /locus_tag="ACSIGJ_001039"
     CDS             complement(16944..17129)
                     /gene="ccoS"
                     /locus_tag="ACSIGJ_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HHQ1329921.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKEKENDGR"
     gene            complement(17129..17428)
                     /locus_tag="ACSIGJ_001040"
     CDS             complement(17129..17428)
                     /locus_tag="ACSIGJ_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HLGFF motif protein"
                     /protein_id="HHQ1329922.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARLQSGRFAVKLEDGTGN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLIGNM"
     gene            17779..18975
                     /locus_tag="ACSIGJ_001041"
     CDS             17779..18975
                     /locus_tag="ACSIGJ_001041"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HHQ1329923.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELEAHGLDSRIGAIGHRVVSGGELYNESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETAHFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITDMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            complement(19489..21330)
                     /locus_tag="ACSIGJ_001042"
     CDS             complement(19489..21330)
                     /locus_tag="ACSIGJ_001042"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0047134 - protein-disulfide reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HHQ1329924.1"
                     /translation="MRTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELTVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVHHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDGRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPAFMLLVNGRRQKRRPKA
                     VAFALGSILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKFSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAVYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRADGSRSEPLLGFVKADKFIEWYEQNR"
     gene            21421..21891
                     /locus_tag="ACSIGJ_001043"
     CDS             21421..21891
                     /locus_tag="ACSIGJ_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HHQ1329925.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLGALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     PEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            complement(21964..22776)
                     /gene="hpnC"
                     /locus_tag="ACSIGJ_001044"
     CDS             complement(21964..22776)
                     /gene="hpnC"
                     /locus_tag="ACSIGJ_001044"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - geranylgeranyl diphosphate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HHQ1329926.1"
                     /translation="MSVGHYENFPVGSLVLPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLSGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGDLTDYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(22863..23345)
                     /locus_tag="ACSIGJ_001045"
     CDS             complement(22863..23345)
                     /locus_tag="ACSIGJ_001045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HHQ1329927.1"
                     /translation="MAIAKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSSDDAFGEQDSELVRIEDAGAFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGSHGHYHH
                     "
BASE COUNT         5721 a         7022 c         5802 g         5385 t
ORIGIN      
        1 tcgtcttcaa aataccaaga ctttgccgcg ccggggaagc cgcccgtctc tttgagccat
       61 tgcacaatcg tcggattgtg tttcgcgccg ggaatttctt tcaaaccgat gtgccttctc
      121 gcttcggcta tccacggtaa ttctgtcatt tttaatcctt tcggtaaaag acgagatgta
      181 aactttgggt tggcaactcg ttctatgttt taagtcgcaa tcacaatgtg caccgcatcg
      241 ccgaacacgc tgtctttttc agtcgcgttt gccgatacgg taactttccg cgcagatgat
      301 atgttgcccg tcaacacaaa ataaaaaaca aagttgcaac atgctgattt atattgttat
      361 ttttatttac gtttatttac gatatgcaaa tgcacggtta caaagatata tttgcataac
      421 tgtttaattt tattgaattt tattgattca atcagtgtct ttccgcatcg taaggctgcc
      481 cggttttaac aatgtaatag gcgggcttcg ccagtttgcg catgatggca acgatgatta
      541 ccatctttgg cttacccgct tttttcagat tatttattaa tttcggaaat gcgttaaaac
      601 ggtaagcaca aagggcgggc atatacagcg ggaatccgtt ttttgagttt cgcttaagtt
      661 caataaatcc tgaaacttta atctcgccgt gaaaatgaat tttctatttc aatttgtcat
      721 atatagttaa tttttttagg gtattttatt ttttgttcca aagtcaaaat atgccgtccg
      781 aacgttcggg cggcagacaa aacggcactg cccgataaag gcagtgccgt tgtccgtttt
      841 aaaccgtgaa acatcagccc aaatcaaagg ctttatgcaa taccctggtt gccagttcca
      901 tgtatttttc gtcaatcaat acggaaactt tgatttcgga ggtggaaatc atttggatgt
      961 tgatgccctc ttcggcgagc gtgcggaaga ttttggcggc tacgccgacg tgcgaacgca
     1021 tacccaaacc gactgcggag actttgcaca cggtgtcgtc gccgtcgata gaagctgcgc
     1081 cgatactgtc tttacgttcc gacaggattt ccaaagtctg tttgtaatcg ccgcgcggta
     1141 cggtgaaaga aaaatcggtt gtgccttcgc tgccgacatt ttggataatc atatcgactt
     1201 cgatgttggc atctgcgacc gcgcccaaaa tctgataggc gacgccgggc ttatcgggca
     1261 caccgcgcac gttgatgcgg gcttggtttt tatcgaatgc gataccggtt acggcagctc
     1321 tttccatgtt gtcgtcctct tcaaaggtaa ttaaggtgcc gttgccgccg tcttgcaggc
     1381 tgctcagtat gcgcaggcgc actttgtatt ttccggcgaa ttctactgaa cggatttgca
     1441 aaactttcga accgaggctg gccagttcga tcatttcttc aaatgtaacc gtatccatac
     1501 ggcgcgcttc gggtacgacg cgggggtcgg tggtgtaaac gccgtctacg tcggtataga
     1561 tttggcattc gtccgctttg agggctgcgg caagcgcaac ggcggaagtg tcggaaccgc
     1621 cgcgtcctag cgtggaaata ttgccttcac tgctgatgcc ttggaagccg gcaacgatga
     1681 cgactttgcc ggcggcgagg tcggcgcgca ttttttcgtc atcaatgctt tctatgcggg
     1741 ctttggtgtg ggcggtatcg gttttgaggg agacctgcca gcctgtgtag cttttggcat
     1801 ccacgccgat atccttcaat gccattgcca aaaggccgat ggttacttgt tcgccggtag
     1861 ctaagacgac gtccagctcg cgcggatcgg gatgctcctg catttcgtgc gccaacgcaa
     1921 ccagacggtt ggtttcgccg ctcatggcgg atacgacgac gacgatatcg tgtccttcgg
     1981 cgcgggcttt agcgacacgt ttggccacgt ttttaatgcg ctcgggcgag cctacggatg
     2041 tgccgccgta tttatgtacg attaacgcca tgtttcatgc tttcttgtgt gggattggtc
     2101 gggcagcatg gtttgctgga aaagcggcta ttattactat tttttacatg aagttcaaga
     2161 acggactgcg ctttcccgcc tgccgtttga cagcggtcag cgaaaaacct gttctttcag
     2221 atttttgaca aaatgccgtc tgaacggttt tcagacggca tacggacagt aattaagcaa
     2281 cggacaacgc attttgctga taacgcagca gttcgcctat gcctttttgc gccagtgcaa
     2341 ccagtttacc caactcgtcc aaactgaatg gcgcgccttc cgccgttccc tgtatttcga
     2401 tgattttccc cgatgcggtc atgacgatgt tcacatcact gtcgcagccg gagtcttcgg
     2461 gataatccaa atccaaaagc ggcacgccgt tcactacgcc cgctgatacg gcggcaacag
     2521 cttcaaggat ggggttttca ctcaaaatgc cgtctgaaac cagtttgccg acggcgattt
     2581 gcagcgcgac aaacgcaccg gtaatcgaag ccgtacgcgt gccgccgtcc gcctgaatca
     2641 catcgcaatc aatcaggatt tgtcgttcgc cgagtttttc catatccacg accgcgcgca
     2701 gcgaacgccc gatcaagcgt tggatttctt gtgtgcgtcc cgactgtttg cccgccgaag
     2761 cttcgcggcg catacgcaaa gcggttgagg caggcagcat accgtattcc gccgttaccc
     2821 agccttggtt tttgccgtgc agaaacggtg gggcattttc gtccacggaa gcggtacaaa
     2881 tcactttggt attaccgcat tcaataaggc acgaaccgtc cgtatgcggc aggaaatgag
     2941 gggtgatttt gatatcgcgc aggctgtcgg cggcgcgtga ggtgcggata taatcaggca
     3001 tactgccctc ccgttaaaaa cagataaatt aaaaagcctt aaatatgaaa aatcacattt
     3061 aaggccttca aactgaaaat ttctacgcct cttcggcttt gctacggata atcaaaagcg
     3121 gcaggtggct ttggcgcatt accgtttcgg cgaaactgcc catcaaaagg tgcatcagcc
     3181 cggtgcgccc gtgcgtgccc aacaccagca ggccggcacc gttttcatcg gcataatcga
     3241 ccaaatcctg cgccatttcg cgcgcaccct tattggcaac cagcaggtgt ttgacggtat
     3301 ttcccacacc cagttcccgg gcggtgcgct cggcggcatc caaaacctcg ttgccttgcg
     3361 cgacggcggc ggcctcgtaa ctttcgtgtt gcaaaaattc gggggcgagc gccatatatt
     3421 cggcagggtt ggcaacgtgt accaaagtca gacgcgcacc gttgaccccg gcaagctcgg
     3481 cggcgtgttt cagggcattg atggacgttt cactgccgtc aacggcaacg accagatgtt
     3541 tgtacatatc gtattctcct tttgcaccgt ctcacggtgc cgtctcttca ggtttgcgca
     3601 aaggcagtct gcgccgtttc attatagacc cgccgtcggg ctttatacaa cagccgaaca
     3661 gcctgactgc tttccagtat aatatgccgc tttcgtgcag tcaggcattt tttgccggct
     3721 ttcgttcact ttttgatttg acgcaatctt gcaggattcg accatgtccg acaacgcttt
     3781 gacctcttcg cgacgcttcg gcggcatcgc cagactctac ggagactctg ccttgacgca
     3841 cttttcacag gcacacgtct gcgtggtcgg cgtgggcggg gtcggctcgt gggcggtcga
     3901 ggctttggcg cgcacgggca tcgggcgttt gactttgatt gatttggaca acgttgccga
     3961 atcgaatgtc aaccgccagc tgcacgccct gaccggcgac ttcggcaaag caaaagttac
     4021 cgccttgcgc gaacgtatta cacaaatcaa tccgcaatgc gaagtatttg aaattgaaga
     4081 tttcgttacc gaagacaatt tgccggaata cttcggaaaa ggttttgatt tcgtcatcga
     4141 cgcgatcgac caagtgcgcg tcaaagcggc aatggcggct tattttgtgg aacgcaaaca
     4201 accgtttgtc ctcagcggcg gcgcgggcgg acagaaaaat cccgccctga ttcaaaccgc
     4261 cgatttaagc cgcgtaaccc acgacccgct gcttgccaac ctgcgctaca ccttgcggaa
     4321 acgctacggc ttcagccgcg atactaagga aaaaatgcgc gtgccgtgcg tgtattcgac
     4381 cgaaaatatc atgccgccgc aatcgggcgc agcgtgttct gccgatgccg cgccgcaggg
     4441 cttgtcatgc gccggctatg gcgcaagtat gctcgttacc gcttcgttcg gactgtattg
     4501 cgcacaggcg gcggtggaac acatcgcggg caaaaaataa gcaatgccgt ctgaaacagg
     4561 attcagacgg catttgaaca aacgcggcaa tcaagctgtc ggttcagaaa aaccaacatc
     4621 ggcacgccta aaaccgtatt cacaatctac tccttttcaa aatatttgca tttaaaagcc
     4681 gttataatgc cgtccgaaca tctgcccgac cacattatgc gtgaatgccg gcagattgtt
     4741 ttcttttgta aacttatatt aaaattcact taccaattca cgccatgccg cccatccctg
     4801 ccctatccgc accatccgag cacactgtcg catgggtatt cggccaaccc gttaccgatt
     4861 tgccgcagga tttgtttatc ccgcccgacg cgttgaaaat cgtgttgggc agcttccaag
     4921 gccctttgga tctgctgctc taccttatcc gcaagcagaa catcgatgtt ctcgatattc
     4981 cgatggtgga aattaccggg cagtatctgc actatattgc ccaaatggaa gcctatcagt
     5041 ttgatttggc ggcggaatat cttttgatgg cggcaatgct gattgaaatc aaatcgcgcc
     5101 tgctgctgcc gcgtaccgaa gccgtcgaag acgaagaggc cgacccgcgt gccgagttgg
     5161 tgcgccgtct gcttgcctac gagcaaatga aactggcggc gcagggtttg gacgcgctgc
     5221 cgcgtgcggg acgggatttc gcgtgggctt acctgccgct ggaaattgca gccgagacga
     5281 agctgcccga ggtttacatc gccgatttga tgcaggcatg gttgggcatt ctttctcggg
     5341 caaaacatac gcgcagccac gaagtaatcc aagaaaccct ttccgtgcgc gcgcaaatga
     5401 cggcaatcct gcgccgtttg aacgaacacg ggatatgcag gtttcacgac ctgttcaatc
     5461 ccgaacaggg cgcggcttac gtgatcgtca acttcatcgc cctgttggag cttgccaaag
     5521 aaggattggt cggaatcgta caggaagacg gtttcggaga aatccgaatc agcctcaatc
     5581 atgagggggc gcattcagac ggcattttcg gcacacgggg cgggcgcgat gtgttctaat
     5641 acgccccaag ccgccaccag aaatcgggag acacgccata tgaccggcat catacattcg
     5701 ctgctcgaca ctgacctcta caaattcact atgctgcaag tagtcctgca ccagtttccg
     5761 cagacgcaca gcctttacga attccgctgc cgcaacgtct cgaccgtcta tccgcttgcc
     5821 gacatcaggg aagacttgga agccgaactc gacgcgctct gccggctgcg cttcacccac
     5881 gacgaactcg gctatctgcg ctccctgcgt ttcatcaaaa gcgactttgt cgattatctc
     5941 gaactcttcc agctccaacg ccgctttgtc gaagccggca cagacgataa aggccgtctg
     6001 aacatccgta tcgaaggccc gatgatacag gcgatgtttt ttgaaatctt catcctcgcc
     6061 attgtcaacg aactttactt ccgccggctg gaaacccctg ccgttatcga agaaggcgaa
     6121 cgccggcttc aagccaaagc cgcgcgcctg aaagaaatcg ccgccgcaca gaatccggac
     6181 gaaccgccct tcctgatttc cgacttcggc acgcgccgcc gctacaagct cgcgtggcag
     6241 gaacacgtca tccgcaccct gctcgaagcc gccccaagca tcgtgcgcgg cacaagcaac
     6301 gtttacctcg ccaaaaaact cggcatcacc cccatcggca ccatggcgca cgaattcctc
     6361 caagccttcc aagccctcga cgtgcgcctg cggaatttcc aaaaggccgc gctcgaaagc
     6421 tgggtgcacg aataccgggg cgatttgggc gttgccctga ccgacgtggt cggtatggat
     6481 gccttcctgc gcgatttcga cctctatttc gccaaactct tcgacgggct gcgccacgac
     6541 agcggcgacc cttacgtttg gggcgacaaa gcctacgccc actatcaaaa gctcaaaatc
     6601 gacagccgca ccaaaatgct gaccttctcc gacgggctgg acatcgaacg ctcttgggca
     6661 ttgcaccaat atttcaaagg ccgcttcaaa accggcttcg gcatcggcac caacctcacc
     6721 aacgatatgg ggcatacgcc cttgaatatc gtgttgaaac tggtcgaatg caacgggcag
     6781 tccgtcgcca agctctccga ctcgccgggc aaaaccatga ccaacaacag caccttcctc
     6841 gcctacctgc gccaagtgtt cggcataccc gaacctagaa cgccgtaaac cggcagaaaa
     6901 agcgtacaat tcctgtttcc gccgcataaa atcttttaaa ataccgcctg atttgaattt
     6961 aaccgaaaga ccgaacttca tgaacctaca tcaaaccgtc gaacacgaag ccgccgccgc
     7021 ctttgccgcc gcaggcatcg ccggcagccc cgttgtcttg cagccgacca aaaatgccga
     7081 gcacggcgat ttccaaatca acggcgtgat gggggcggcg aaaaaagcca aacaaaatcc
     7141 gcgcgaactg gcgcaaaagg tcgccgacgc attggcgggc aacgccgtca tcgaaagcgc
     7201 ggaagtggcc ggacccggct ttatcaacct gcgcctgcgc cacgaatttc tcgctcaaaa
     7261 cattcatgcg gctttgaacg acgcgcgttt cggcgtagcg aaaactgccc aaccgcaaac
     7321 cgtcgtcatc gactattcct cgcccaatct ggcgaaggaa atgcacgtcg gccatctgcg
     7381 ttccagcatc atcggcgaca gtatttcgcg cgtgttggaa tttacgggca acaccgttat
     7441 ccgtcaaaac cacgtcggcg actggggtac gcagttcggc atgttggtcg cttatttggt
     7501 cgagcagcaa aaagacaatg ccgcgttcga gctggcggat ttggagcagt tttaccgcgc
     7561 cgccaaagtg cgctttgacg aagaccctgc ctttgccgac accgcacgcg aatacgttgt
     7621 gaagctgcaa ggcggcgatg aaaccgtgtt ggcgttgtgg aaacagtttg tcgatatttc
     7681 gctctcgcac gcccaagccg tttacgacac gctgggcttg aagctgcgcc ccgaagatgt
     7741 ggcgggcgaa tcgaaataca acgacgattt gcagcccgta gccgatgatt tggttcaaaa
     7801 aggcctggcg gttgaggacg acggcgcgaa agtcgtgttc ttggacgagt tcaaaaacaa
     7861 agagggcgaa cccgccgcat ttatcgtgca aaaacaaggc ggcggcttcc tttatgcctc
     7921 caccgacttg gcgtgcctgc gctaccgcat aggccgtctg aaagccggcc gcctgctgta
     7981 cgtcgtcgac caccgccaag ccctgcactt cgagcaactc tttaccactt cccgcaaagc
     8041 aggctatctg cctgaaaatg caaaagccga gtttatcggc ttcggcacca tgatgggcaa
     8101 agacggcaaa ccgttcaaaa cgcgcagcgg cgacaccgta aaactggttg acctgctgac
     8161 cgaagccgtc gagcgcgcca ccgctttggt gaaagaaaaa aatcccgaat tgggcgcgga
     8221 tgaagccgcc aaaatcggca agaccgtcgg catcggcgca gtcaaatacg ccgatttaag
     8281 caaaaaccgc accagcgatt atgtgttcga ctgggacgcc atgctctcat ttgaaggcaa
     8341 caccgccccc tacctgcaat acgcctacac ccgcgtgcaa agcgtgttcc gcaaagccgg
     8401 agaatgggac gcaaccgcac cgaccgtttt gaccgaacca ctggaaaaac agcttgccgc
     8461 cgagcttctg aaatttgaaa acgtgctgca aagcgtggcg gacaccgcgt atccgcacta
     8521 cctcgccgcc tacctttacc aagccgccac cctgttcagc cgcttctacg aagcttgccc
     8581 gattttgaaa gccgaaggcg caagccgcaa cagccgcctg caactggcaa aactcaccgg
     8641 caacacgctg aaacaaggct tggatttgct gggcatcgat gtgttggacg taatgtaaaa
     8701 ccgcaccgcc cgattgcgga caacagccct gcaatcaacc ggcggaccat cctcatccga
     8761 atctgaaaaa gcggcgcgat acaccgtatc cgccgcccct cccaaaatgc gaaacagaca
     8821 aacgccaagc aagcaagcaa gcaagcaagc aagcaagcaa gcaagcaagc aagcaaaaaa
     8881 ttataccccc ccttcctgcc gacgcacgca ctttccgcgc ggcgtattcc ccttttcccg
     8941 cccctcaaac ccgccttttc ttcaggcagg gtttcagccc gcctcctttc cctgttttcc
     9001 ttccctcaac acgcgtgctc gccccctgcc gcaccgtgcc gcactttcgc gcccggacgg
     9061 catcgttccg ccatccggtt ctctgtttca aatacccctg tttcagaaag aaatgcagat
     9121 gtttcaacac acaggacgac atataaagta ccgccctatg tgtcgtcctg atttggaagg
     9181 ggttgcgccc cttcctaaat atagtctgat cctgccgccc cgaaggacag atgtccgagt
     9241 ggcggggttt caaccgaaaa ggaaatacga tgaatattca cgccctgctc tccgaacaat
     9301 ggacgctgcc gccattcctg ccgaaacggc tgctgctgtc cctgctgata ctgctggccc
     9361 ccaatgcggt gttttgggtt ttggcactgc tgaccgccac cgcccgcccg attgtcaatt
     9421 tggactacct tcccgccgcg ttgctgatcg ccctgccttg gcgtttcgtc aaaattgccg
     9481 gcgtattggc gttttggccg gcggttttgt ttgacggact gatgatggtc atccagctct
     9541 tcccttttat ggacctcatc ggcgccatca acctcgtccc cttcatcctg accgcccccg
     9601 ccccttatca gataatgacc gggctgttgc tgctgtatat gctggcgatg ccgtttgtgt
     9661 tgcaaaaagc cgccgtcaaa accgacttcc gacacattgc cgtctgtgcc gccgttgtgg
     9721 cggcagccgg ctatttcacc ggccatttga gttactacga ccgggggcgg atggccaata
     9781 tcttcggcgc aaacaacttc tattacgcca aaagtcaggc gatgctctac accgtcagcc
     9841 agaatgccga ctttattacc gccggcctgg tcgaccccgt cttcctcccc ttgggcaatc
     9901 agcagcgtgc cgccatgcgg ctgagtgagc cgaaatctca aaaaatcctc tttatcgtcg
     9961 ccgaatcttg ggggctgccg ggcaatcccg agcttcaaaa cgccactttt gccaaactgc
    10021 tggcgcaaaa agaccgtttt tcggtttggg aaagcggcag ttttcccttc atcggcgcga
    10081 cggtcgaagg cgaaatgcgc gaattgtgcg cctacggcgg tttgcgcggg ttcgcactgc
    10141 gccgcgcgcc cgacgaaaaa tttgcccgct gcctccccaa ccgtttgaaa caagaaggtt
    10201 acgccacctt tgcgatgcac ggcgcgggta gttcgcttta cgaccgcttc agctggtatc
    10261 cgagggcggg ctttcaaaaa atcaaaaccg ccgaaaacct gatcggtaaa aaaacctgcg
    10321 ccattttcgg cggcgtgtgc gacagcgagc tgttcggcga agtgtcggca tttttcaaaa
    10381 aacacgacaa gggactgttt tactggatga cgctgaccag ccacgccgac tatcccgaat
    10441 ccgacatttt caaccacagg ctcaaatgca ccgaatacgg cctgcccgcc gaaaccgacc
    10501 tctgccgcaa tttcagcctg cacacccaat tcttcgacca actggcggat ttgatccgac
    10561 gccccgaaat gaaaggcacg gaagtcatca tcgtcggcga ccatccgccg cccgtcggca
    10621 acctcaatga aaccttccgc tacctcaaac agggacacgt cgcctggctg cacttcaaaa
    10681 tcaaataaca acaatgccgt ctgaacgcac caacagcctt cagacggcat tttgcagaca
    10741 gaccgacctc ctcaagccca cttttttcat catctccgat aaattgcttt gtaagaatcc
    10801 aaaaatccaa aatcacagga agttatcaaa aaaacagaaa ccatccgccg tcgttcccgc
    10861 gaaagcggga atccagaccc ggaaaccgca gaaatttatc ggaaaaaccg aaactcgaaa
    10921 acaacggaca aaattcccgc ctgcgcagga atgacggctc atacatcacc caaaaaaaat
    10981 tgaaaccaaa cagctgggat tccctcttgc gtgaggctga cagatgccgt ctgaaagact
    11041 ttcagacggc atagggcgtt ttctctttga atttaaattt tgccacaaaa aagccgcccc
    11101 aaatgaatac ccgggcagct ttttgttgat atgactccaa tcagcggtgt tgcggattgt
    11161 aacgtttttc caaacgcagg aatatccagc ctaagaaagt cgtcatcaac agataaatca
    11221 gggcggcggt gtaaagcggt tcttcataaa ccgaataccg gcccgtaatc gtattctgaa
    11281 catacgccaa ctccgccaca gcaatgaccg acagcagcga gctgtctttc aagagcgtga
    11341 tgaactcgct cgccaaaggc ggcagcatac ggcgcaatgc ctgcggcaga atcacatagc
    11401 gcatcgcctg cggataggtc agtcccaaag aacacgccgc ctccatctgt cctttgtcta
    11461 tagactggat gcccgcgcgg aaaatctcac agatatacgc ccccgagttg gcgatcagtg
    11521 ccaaagaacc ggcaatcagc ggcccgtatc cgcgacgcag cgcgattgcc gcctcgccgc
    11581 tgaccaaaat gccgtctgaa ggatggacga aaaacggaaa ccacacatac gcccaaatca
    11641 caatctgcac aaacagcggc gtaccccgga acagggtaac gtacagcagc gaaaccttac
    11701 gcaacgccca cgccagcacg cgcatcggcg caccggcttt ttccaagtga atcaggcgcg
    11761 ccaacgccaa caacagaccc aataccgaac cgcccgccgt cgccacgacc gtcagcccca
    11821 aggtcgtcag tgcgccgtaa agaaacatcc agcggtattc gtaaataatg tcaaaacgaa
    11881 aatccataaa ccgtccgtat caaaaaccgg cggaactgcc gccgttgcaa aataatccgc
    11941 cattttaccg taaaaaccgc cgcctgaact tttttatcgc ggcagacggc ggttgcgcgt
    12001 ctccgcaaaa atgcagggcg cgcggttttc agacggcatt tgccgttcaa ggccgtgcgg
    12061 tgtctttacc aaatgcccaa ccattcgccc acggaatcca tccaatcctt attgcccccg
    12121 ccgctcctgc ctgcccggcg gtacgcccac ggcgcttgcg gatttttagc tttccacaat
    12181 cctttgcgtt ccctttccgc ctgaatttga gcgtcggcat agtcggcaaa atccgcctta
    12241 tcctgctgtt ctttagcata acttttataa tgccacgccg ccccgtcctg cacctgcatc
    12301 aggttcaaat cggttttgcc ggcggatacc tgcgccactt cgcgctgata gcggtcggtt
    12361 tcaaacacac gtacactgac tttcctaccc tccgccgccg cgcgcaggtt gtcgcgcgaa
    12421 cgtgtaccgt aagcctgttt catctccggt gcgtcgatat acgccatccg aattttatgt
    12481 ttcgcgccgt cgccgtcgat gacgtgaagg gtatcgccgt catagacttt ggacaccgtg
    12541 cctgtgtagc tgtggccgga tttcgccgat gcccgtcggc gaacgggcgc gtcggaaccc
    12601 acgtcccctg ccgtgccgag tacgtcgagt acggcaaccg ccgtccgcac cgcctcactg
    12661 tcatatcccg tataacccaa cgcgcccaaa agcgacaggg cgacgggaag ccatttcatg
    12721 atttttttaa tctgcatatt tttcaaatgc cgatgccgtc tgaacatctc tgaaccggat
    12781 ttcagacggc atcttaacgt caggattacc cttggcaggg atagatgact ttcgcaccct
    12841 cttccgtccc caaaatcaac acatcggcgg catcgcgggc gaatatgccg ttttcgagta
    12901 cgccggtgat tttgttgatt tcatcttcca tcgtcagcgg ctgatcgata ttcaagccgt
    12961 ggacatcgac gatttggttg ccgtaaaacg tggtgtagcc gatacgcagt tcgggctgtc
    13021 cgcccatggc aagcagtttg cgcgaaacaa gggagcgcgc gctttcgacg gcttccacgg
    13081 gcagtgggaa tttgcccagc cgggaaacat atttgctttc gtccgcaatg cagacgaatt
    13141 tttctgatgc gctggcgacg attttttcgt tgaggtgcgc gccgccgccg cccttaatca
    13201 tttgcagggc gtggttcact tcgtccgcac cgtcgatata gaccgccaaa ccggacactt
    13261 cgttcaggga cacgacggga atgtcgtacc gggcaagcag ttcgccggat tttttggagg
    13321 ttgaaaccgc gcctttgatt tttttaccgc ttttgcccaa ggcttcgatg aagaaattga
    13381 tggtcgaacc ggtaccgatg ccgatgtatt cgttttcggg tacgaattcg actgcttttt
    13441 cggcggcgat gcgcttgagt tcgtcttgtg tcgtcatatt tttatccttt gggaaaccgt
    13501 atcaacaaac agccgccatc ttaacatttt tttgcacgtc ctgcccgccg cgttcaaatc
    13561 cgtaccagca ataccgccgc ctgcgcctct atgccttcca tccgcccgag atagccgagt
    13621 ttttcgttgg ttttgccttt gatattgacg cacgaaatat cgatacccaa atcggcggcg
    13681 atgttggcac gcatttgcgg aatgtgcggt gcgagtttgg gtttctgtgc aatcacggtc
    13741 gtatcgacat tgaccgcctg ccaaccctgc gcctgaacgc tttgatacgc cgcacgcaaa
    13801 aggacgcggc tgtccgcatc tttgaactct gcggcggtgt cgggaaaatg gctgccgatg
    13861 tcgcccaaac ctgccgcacc gagcagcgcg tcggtaacgg cgtgcagcag cgcgtcggca
    13921 tcggaatgcc cgagcagccc tttttcaaat gggatttcaa ctccgccaag tatcagcttt
    13981 ctgccttcga tcaattggtg gacatcgtag ccctgtccga tacggatatt cgtcatcgtt
    14041 tgtgttcctg atgttttgaa ttgaatttca gacggcattg agcagcagcc tgacgatgta
    14101 tgcgtcctgc ggctgcgtca gtttcaaatt gcgcgcgtcg ccctgtatca gtagcggacg
    14161 cacacccagt ttttccacgg cggacgcttc atcggtaatg ccgtccaagt tttccgcagc
    14221 caatgcgcgg tgcagcaaac ccgcttgaaa aagctgcggc gtttgcgcct gccaaagccc
    14281 cgaacggtcg acagttgcac tgatttgtcc gctttctgcg cgcttgagcg tatcggcaac
    14341 gggaactgcc aaaatcccgc cttcggcggc gttgcccgcc tgttctatca accgcgtcag
    14401 agcttcagac ggcaggcagc agcgggcggc atcgtgtacc agaatattgt cggtttccgc
    14461 cgccaaaccg gtttccaaca gttttgccac accgttgcgg acagtttcgg cgcgggtctg
    14521 tccaccgttt ttccacaccc gaacctgtgg aaatgccgtc tgaaccttat cggcaaacgt
    14581 gtcttcgggc gagacgacaa cgacggtcaa atcgacggcc tcatgccgtt caaaaatccc
    14641 aagtacatgt tctaaaacgg ttttgcttcc gatttcgaca tattgcttgg gtttgtccgc
    14701 accgaaacgc accccgatgc cggcggcggg aatcagcgcg atatttttgc gtttcatgcg
    14761 ttttccccgc cgttttcaga cggcacggct tccttacgcc aaatacaggc ttcgcccaag
    14821 ccgtcaagat attcttcgtg cgccgccagt tcggcttcat ccgccttaat cactttcagt
    14881 ttggcaccga gtttcatttc ggcagatgca acaggtttgg ctgtcggttc ttcctcctcg
    14941 gtctccccca tcaagtcgaa ttgccgccgc gtcatcgcca gatagacttc gcccaaaagt
    15001 tcgcagtcga tcaatgcgcc gtgcaggacg cgcttgctgc ggtcgacgga aaaacggttg
    15061 cacagggcat ccagactggc tttctgcccg gggaacattt cgcgcgccat cgccagggta
    15121 tcggtaacgg tgcagccgag ttcctcgacg gtcggcagcc ccatccggcg gaactccata
    15181 ttgaggaagc ccacatcgaa tttggcattg tggataatca gttccgcacc gcgcagaaaa
    15241 tcggcaatct gcctgccgac ctctgcaaac ggcggcgcgt ttttctcttc caaaacctgt
    15301 atcgtcaagc cgtggacgcg tgccgcctct tcgggcatat cgcgctcggg gtggacatag
    15361 aggtgcaggt ttttgtcggt catttggcgg ttgaccattt ccagaccggc aaactcgacc
    15421 agacggtcgc cgccgtcggc atacaggccg gtggtttcgg tatcgaggat gatttggcgt
    15481 gtcatcatat cggtgtcttt cttctgtctt cgtaaattga ttatttttta agcaatgtat
    15541 ttttctgttt tcatttcaat gcacaaaccc acttattcac agtgtgttca caacattggg
    15601 caggcggatt gtgtattttg gggacaattt tttcagacgg cattcaaggt tttttcctga
    15661 ttgccgccgc gcctaaaaac cgcctttcgc gcttaatcaa aaataccgcc aacggaatat
    15721 tgcccaaggc gacaatcaga tacagcaggg aaatgctgtc aaacaaaaac agcaataccg
    15781 cgctcaaaac ggcggcggaa accataaaga tgccgttaac gatattgttg gcggcaacgg
    15841 cgcgggcgcg gaaagtctcg ctgctggcgg tttgcagcca ggtatagagc ggaacggaga
    15901 aaaatccgcc gaaaaagccg atcagcgtca tcaccgccat cacggagtat gcccatcctt
    15961 gcgataaaaa ccaaaaaatg ccgttcagcc cttcaaaacg gtgtccgtgc gtcagccaca
    16021 ccaaaaccag gccgcaaacc gtcaaaccca acgcaccaac cgttacccaa gccaacatca
    16081 gccgttccct gccgaacttg gcacacagta ccgaaccggc ggcaataccg atggaaaaca
    16141 aagcaagcat caggttaaaa acattatcgt tgccgcccaa atggatttgg gtaaaggtcg
    16201 gcagttgcgt ggtataaacc gcgccgacaa accaaaacca cgagatgccg ataatggcgg
    16261 taaaaacggg attgtgccgc accgtttcac gcagcaggga ttttgtacca cggacaatat
    16321 tccactcgat ttgggtatcg gcagccttgg cgggtacgga cggcataaac aggctgccgg
    16381 ccgttcctcc gacggcgacc agcaaaacca gtatcccgac aatataaggc ggcgcgccgg
    16441 caaccgccgt tcccaaaatc tgaccaaaca ggatggcgac aaacgtaccg gattcaatca
    16501 ggctgttgcc catcatcagc tcgttgtcgt cgagataatc gggcagaatg gcatatttaa
    16561 gcgggccgaa cagcgtcgat tgcgcgccca tgcaaaacag gcagagcaag agcagcgggg
    16621 ctgaccggat ataaaacccg tatgccgcca ccgccataat gatgatttcc agcaccttga
    16681 cccaacgcgc caaaacggcc ttgtcgaatt tgttacccaa ctgccccgac agcgcggaaa
    16741 acaggaaata cggcaaaata aacagcagtg cgcccaagtt caacatctgt ccggcaggca
    16801 gaacgccgtt ttgccccaaa ccgtaaaacc cgatcatcac aaacagcgcg gttttgaaca
    16861 cattgtcatt gaacgcgccg agaaactgcg tagcgaaaag aggcccgaaa cggcggcttt
    16921 taaccagtcc caaaccgcct tttttagcgt ccatcgtttt ccttctcttt ttcaatcagt
    16981 ttacttgtcg aatcatcatc catcaaaatg cggtgcgccg gtccttccaa atcgtcaaac
    17041 tgtccgtttt tgcccgacca ccagaaaaac cagccgatga caaatgccaa aataatgctg
    17101 atgggcacca gaataaacat actttccatc acatattccc tatcaaatcg ttcaaaacaa
    17161 aagtctgccc tgacacggtc agatattcgt tacgcaaagt tccgacgggg gcttcgtcaa
    17221 aaaacagctc gatacggtct ttgaccacgc gccaatattg gggaatttcc gtctgaccga
    17281 acggcgacag gacatggttg cccgtcccat cttccaattt aacggcaaaa cgcccgctct
    17341 gcagccgtgc ttccgattcg tcgtcggcaa gaaggatgaa aaagcctata tgccgtcccg
    17401 attggtcatg aatactgaaa taatgcataa attccccacc cgcctttttt cagacggcat
    17461 caactaaaaa cagggcgaat gtaccagttt ggacgggaaa aatgcaaaga aattctccct
    17521 cccccagccg aaaacaccgg caaaccgcat atcccccttt tttccgtcaa aatacccgac
    17581 ttccgccatt ttcacgcaaa cgcccgatta agccaagcaa ttgcaaagat tttttgctag
    17641 aatagcctgc ttcttttatc aaccgtttca gacggctcca ctactttccc gtcaaggaaa
    17701 gcaaagcgga ttcggcacga atccgattag tatccgtgtc cgattccaat gccgtctgaa
    17761 actttccgga gtaagaaaat gtcccaaaaa ttgatcttgg ttttgaactg cggcagctcg
    17821 tccctcaaag gcgcggtcct ggataacggc agcggcgaag tcctgctgag ctgccttgcc
    17881 gaaaaactca acctgcccga tgcctacatt acattcaaag taaacggcga aaaacacaaa
    17941 gtcgatctgt ccgcacaccc cgaccacacc ggcgcggtcg aagccctgat ggaagaactc
    18001 gaagcccacg gcctcgacag ccgcatcggc gccatcggcc accgcgtcgt cagcggcggc
    18061 gaactgtaca acgaatccat cctcgttgac gacgaagtca tcgccggcat cgaaaaatgc
    18121 atcccgctgg ctcctctgca caatcccgcc cacctcttgg gcctgcgtgc cgcgcaaagc
    18181 attttcaaag gcctgcccaa cgtcgtcgta ttcgatacct ccttccacca aaccatgccc
    18241 gaagtcgcct acaaatacgc cgttccgcag gagttgtatg aaaaatacgg cctgcgccgc
    18301 tacggcgcgc acggtaccag ctaccgcttc gttgccgacg aaaccgcgca cttcctcggc
    18361 aaagacaaaa aagacctgcg tatggtcatt gcccacttgg gcaacggcgc atccatcacc
    18421 gccgtcgcca acggagaatc gcgcgacacc agtatgggcc tgaccccgct ggaagggctg
    18481 gtaatgggta cgcgcagcgg cgacatcgat ccttccgtat tcggcttcct cgccgaaaac
    18541 gccaatatga ccattgccca aatcaccgac atgctgaaca aaaaatccgg cctgctcggc
    18601 atttccggcc tgtccaacga ctgccgcacc attgaagaag aagccgccaa ggggcataaa
    18661 ggcgcgaaat tggccttgga tatgtttatc taccgccttg ccaaatacat cggcagtatg
    18721 gcggttgccg caggcggctt ggacgcactg gtctttaccg gcggtatcgg cgaaaactcc
    18781 gacatcatcc gcgaacgcgt aatcggctac ttgggcttcc tcggtctgaa catcgaccaa
    18841 gaagccaacc tgaaagcccg cttcggcaac gccggcgtga ttaccactgc cgacagcaaa
    18901 gccgttgccg tggtcattcc gaccaacgaa gagctgatga ttgcccacga taccgcccgt
    18961 ttgagcggtc tgtaaggttt tatcctcacg cgaactgcct ccggaaacgg aggcagtttt
    19021 tttatagtgg attaaattta aaccggtaca gcgttgcctc gccttgccgt actatttgta
    19081 ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactatatc cggctttcca
    19141 tgcttaaaca gcactacctc ttttcagaca ttgacggttg cagccgccta cctgaacctt
    19201 aatgattgac tattttttaa tcatgttatt attttccata aaatacatgg cattaagatg
    19261 tttttccaca aaagatacac acaccggcaa acaccggctg tgtttatctt ttcttatgcc
    19321 tattttttaa tcatcgtatt tttatctttt aatttcaata cgcaaaccga cttatacaca
    19381 cggttttcac atctttggac tgcttccgtg tgtatagcgg atattgccgt tttcctgtct
    19441 gacaaaaatg ccgtctgaga acttcagacg gcatttgaaa catcggaatc agcggttttg
    19501 ttcataccac tcgataaact tgtctgcttt gacaaaaccc agcagcggct cgctgcggct
    19561 gccgtcggcg cggacgacaa acacgcccgg cggcccgaac agaccgtatt ctttcaacaa
    19621 cgcctgatgt tcgggcgtgt tggcggttac gtcgatctgg aaaaagcgtt ccatatcgac
    19681 tgcctgatgc acttccggct gattgagcgt gtaaaccgcc atttctttgc aggaaatgca
    19741 ccagtcggca taaaaatcca aaacgacagg tttgtcggga tgttctttca acgccgtatc
    19801 catcgctgcc ttcagcgcgg cagtatcggc aaacattttg ccgtgttccg aaaatttgcc
    19861 tgcttcggcc ggcggattga gggtcaagaa atggtgcagc gcggtcgttt tgccgtttgc
    19921 gccctgccag ccgaaccacg cgccgcctat cagcaatatg ctgcccaatg cgaatgccac
    19981 agctttcgga cggcgtttct gcctgcgtcc gttgaccagc agcataaagg caggaaccag
    20041 catcagcagc gtgtacagcg cgacgacgag ataatagggc aaatgcggcg tggcgaggta
    20101 aacggcgacg gcaagcagga taaagccgaa agcgtatttg acggcattca tccaatcgcc
    20161 cgccttaggc aggatatgtc ctccgaacgt gccgatggca atcagcggca cgccggtgcc
    20221 caacgccaaa gtgtaaagtg ccaaaccgcc caaaaccgca tcgcccgtct gaccgatata
    20281 gcccaaggca aacgccagcg gcggggcgac gcacggtccg acaatcagtg cggacagaat
    20341 gcccataata aagacggaaa cgattttacc gccggagagc ctgctgctct ggttttggaa
    20401 atatgactgt acggcgttgg gaagctggat gttgaacagc ccgaacatag acagcgccaa
    20461 gacgaccatc aaagccgatg ccgccaacac gacccaagcc tgctgcaacc atacggtcag
    20521 cagcgcgccc gtcagtccgg caacaatgcc gaccagcgta taagtcagag ccaaaccctg
    20581 aacataaacg acggacagca caaacgcccg cgccttgccc gcctttttgt caccgaccac
    20641 gatactggac acaatcggca gcaggggata catacaagcg gtaaaactca ggcccaaacc
    20701 ggcgagaaaa aacgccaaaa gattggcgtt gagcgtatcc caagacagct tgaaacggcc
    20761 gtcgccgccc tcgtccccct tcgggggcgg caacgccccg ctgccgtttt gagaggaagg
    20821 ctgcaaaaaa cggtctttgg cggatgccgg ttcgtcggtt tgcggatggt aagtgccgtt
    20881 gccggaaata tcaaactcgg tatccacggg cggatagcac acgccggctt cggcacagcc
    20941 ctgataggtc aaaaccaatt tatacggttc gccgacagcc tttgcataag gaaaggcaac
    21001 ctgcgcctcg tgatggtgaa ccgtctgcct gccgaaaaac tcgtcttcct tctcttcgcc
    21061 cttactgaaa gaaggctgtc ccaacaaatc cgccggatcg gtcttgccga cgattttcgc
    21121 ctgatacata tagtatccgt cggcaatcct gaaacggacg ttcacgccgt cgtcggcaac
    21181 agtaagctcc ggcacgaatg ccttttccgg cggcagcaga tcgttcgcat ccagcgcgaa
    21241 agcccgtccg cacaacatca aaaatacggc gaacaggcaa atcagttttt tcataatcga
    21301 atccgtttca gacaaataat ttgtccgcat tataaatggt aaggttgacg gtaggattta
    21361 atttatgtaa aacccgccat tatccgaacc tatttccata aacatcttat cggacccgcc
    21421 atgtacgatg tcaataccca cgatgtccgc cgctttttcg cccgcgtgtg gcagcagcgg
    21481 ctcaatccgc tgcaattggg cgcgttggaa cagaaagccc tccgcattgt cgaagcccat
    21541 cccgaatacc accgttatct cgaacgcatc gaagaccatc tggacaccga ctggctgccc
    21601 gaaaacggcg aaagcaaccc cttcctgcat atgtcgctgc atttgtccgt ccaagaacag
    21661 gcgggcatag accagccgca cggcatacgc gccatccacg acaccctgtg cgccaaacgc
    21721 ggctggccgg aagccgaaca cgaaatgatg gaggcactgg cggaaacact gtggacggcg
    21781 caacgctacg gcaccggttt ggatgtcaat ttctacatga cccgactgcg caaactcatc
    21841 ggcttgggtg cagaggatca agccaggtta aacccgcatg aaatcgcctg accataccga
    21901 ccgcctgcaa aatgccgtct gaagcggaac aacccctttc agacggcatt cattttcccc
    21961 caatcatttc cacaacgcgc gtttcaacat aatcaaccaa tccttcttat ccaaaacggg
    22021 gcgttgtgca aacacatcgt atcggcacgc gtccagtttc tgcaaaatca actgcgcccc
    22081 caacacaatc atacggagtt ccaaaccgat acgccctttc aattcgcgcg ccaaaggcga
    22141 acccgccttc agcatacgga atgcacgccg gcactcatac gccatcagcc gctgaaacgc
    22201 cgcatccgcc cgtcctgctg cgatctgttc ctcagaaaca ccgaatttca acaaatcgtc
    22261 ctgcgggata taaaccctgc ccttttgcca atccacagct acatcctgcc aaaaattcac
    22321 cagttgcaaa gccgtacaaa taccgtcgct ttgcgctacg cacaccgcat ccgttttccc
    22381 gtataaagcc agcataatgc gtccgacagg gttggcggaa cgccggcaat aatcggtcag
    22441 atcgccgaaa tgcgcgtacc gcgttttaac cacatcctgc gaaaacgccg agagcagatc
    22501 ataaaacggc tgcaaatcca aaccgaacgg cacaaccgcc tcggcatcca atcgtgcaat
    22561 caaaggatgc gccgaccggc cgcccgatgc caacacgtcc aactcgcgcc gcaaaccctc
    22621 caaccccgac aacctggctt cagacggcat actgccctcg tccgccatat cgtccgccgt
    22681 ccgtgcaaac gcatacaccg catgaaccgg cttcctcaac ctgcgcggca aaaccagcga
    22741 accgacggga aaattctcat aatgcccaac cgacatacct tctccatcca tcaaacaaaa
    22801 tgccgtctga aacggaacaa acccttttca gacggcatca gatacctcca agctgccggc
    22861 aatcagtggt ggtaatgacc gtgcgaaccg tggacatgac cgtgtgcgat ttcctcatcg
    22921 gatgcatcgc gcacgctttc aaccgtagct ttaaagcgga ttttcatgcc tgccaaagga
    22981 tggttgccgt ccaccaccgc cttgccgtcg gcaacatcgg ttacacgata gacgacaaca
    23041 tcgccggttt caggatcgtc ggcttcaaac atcatgccga cttcgacttc aacagggaac
    23101 gcgcccgcat cttcaatacg gaccaactcc gaatcctgct cgccgaacgc atcgtcggac
    23161 gaaagcgcca catcgaccgt atcgccggca tccttaccgt gcaacgcctc ttccaccaaa
    23221 gggaaaatgc cgtcgtaacc gccgtgcaga tacgcaatcg gttcttcggt tttatccaaa
    23281 agctgattgt tggcatcata catctcataa tgcagcgaaa ccacggaatt ttttgcaata
    23341 gccatatttg tcctttcagg aacagcagat tgattacagg cgcattctaa cacaaccgcc
    23401 gcgccggccg attaccgtta acctgttcat aaactgtacg gcacatattt caatgtaaat
    23461 ttttgttatt ttattgcagt gtaaattttt tacaacactc tcaaaaacca ttccgaccca
    23521 tctgccgact tccccaatct gccttaataa atcatacaag atactgaatt atattaatct
    23581 ctataatatt tatccctatc ggatttttaa cagcaaaacc gttttacagg atttatcaat
    23641 ccgcccgcca gaaaactttt cattcaaacc tttttcccat ctgtacgaca ttgcaatccc
    23701 ttattccatg gtgcataatt acgcaaattc agcgatgaat ttccaacccg gtttgtagta
    23761 tggtcgaaaa agacctattt gtttcaataa tttaaattga ttttaaaggt tactaaaatg
    23821 aaaaaatccc tgtttgccgc tgctttgttg tctttggctt tggctgcctg cggcggagaa
    23881 aaagccgctg aagctcctgc tgctgaggct tcctctaccg aggcacctgc
//
LOCUS       DBJGGI010000026        22748 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.026, whole genome shotgun sequence.
ACCESSION   DBJGGI010000026 DBJGGI010000000
VERSION     DBJGGI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22748
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.026"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..211
                     /locus_tag="ACSIGJ_001046"
     CDS             <1..211
                     /locus_tag="ACSIGJ_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329928.1"
                     /translation="CRDSLKSGGGGMVLQINDVDTNSGEFAIYTAQDASVKLDLLKME
                     LKHCKEMLEQKDKEIELLRKLTETV"
     gene            300..869
                     /gene="rsmD"
                     /locus_tag="ACSIGJ_001047"
     CDS             300..869
                     /gene="rsmD"
                     /locus_tag="ACSIGJ_001047"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HHQ1329929.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFASADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGMEAASRNAKRVVIADNNRQTVQTLEKNSRELGLG
                     QVQTVCSDGIAYLANLKEKFDVVFLDPPFAWQSWESLFNVLGTRLNDGAYVYIEAGRQ
                     PDKPDWLTGYREGKSGQGTFELRVFQVAE"
     gene            992..1243
                     /locus_tag="ACSIGJ_001048"
     CDS             992..1243
                     /locus_tag="ACSIGJ_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HHQ1329930.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            1340..1423
                     /locus_tag="ACSIGJ_001049"
     tRNA            1340..1423
                     /locus_tag="ACSIGJ_001049"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1374..1376,aa:Tyr,seq:gta)
     gene            1454..1527
                     /locus_tag="ACSIGJ_001050"
     tRNA            1454..1527
                     /locus_tag="ACSIGJ_001050"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1486..1488,aa:Gly,seq:tcc)
     gene            1537..1611
                     /locus_tag="ACSIGJ_001051"
     tRNA            1537..1611
                     /locus_tag="ACSIGJ_001051"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1569..1571,aa:Thr,seq:ggt)
     gene            1660..2844
                     /gene="tuf"
                     /locus_tag="ACSIGJ_001052"
     CDS             1660..2844
                     /gene="tuf"
                     /locus_tag="ACSIGJ_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HHQ1329931.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSS
                     YDFPGDDCPIVQGSALKALEGDAAYEEKIFELATALDSYIPTPERAVDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVG
                     VLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFR
                     TTDVTGAVTLEKGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSV
                     IA"
     gene            2851..2926
                     /locus_tag="ACSIGJ_001053"
     tRNA            2851..2926
                     /locus_tag="ACSIGJ_001053"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2884..2886,aa:Trp,seq:cca)
     gene            3041..3319
                     /gene="secE"
                     /locus_tag="ACSIGJ_001054"
     CDS             3041..3319
                     /gene="secE"
                     /locus_tag="ACSIGJ_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HHQ1329932.1"
                     /translation="MTEHTPEKKNVKVNQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            3321..3857
                     /gene="nusG"
                     /locus_tag="ACSIGJ_001055"
     CDS             3321..3857
                     /gene="nusG"
                     /locus_tag="ACSIGJ_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HHQ1329933.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            3958..4392
                     /gene="rplK"
                     /locus_tag="ACSIGJ_001056"
     CDS             3958..4392
                     /gene="rplK"
                     /locus_tag="ACSIGJ_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HHQ1329934.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMESGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            4392..5087
                     /gene="rplA"
                     /locus_tag="ACSIGJ_001057"
     CDS             4392..5087
                     /gene="rplA"
                     /locus_tag="ACSIGJ_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HHQ1329935.1"
                     /translation="MAKVSKRLKVLRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDSRKSDQVIRGSVVLPKGTGKTTRVAVFTQGVNAEAAKEAGADVVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGIILGPRGLMPNPKIGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGVRVDTSSVNN"
     gene            5315..5815
                     /gene="rplJ"
                     /locus_tag="ACSIGJ_001058"
     CDS             5315..5815
                     /gene="rplJ"
                     /locus_tag="ACSIGJ_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HHQ1329936.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFVELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            5873..6244
                     /gene="rplL"
                     /locus_tag="ACSIGJ_001059"
     CDS             5873..6244
                     /gene="rplL"
                     /locus_tag="ACSIGJ_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HHQ1329937.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEAAGAKVEIK"
     gene            6435..10613
                     /gene="rpoB"
                     /locus_tag="ACSIGJ_001060"
     CDS             6435..10613
                     /gene="rpoB"
                     /locus_tag="ACSIGJ_001060"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HHQ1329938.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVYYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEQESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTNKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKLSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKERRKAGELREFLNKLYNGSGKKEDLDSLTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSEDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            complement(10578..10718)
                     /locus_tag="ACSIGJ_001061"
     CDS             complement(10578..10718)
                     /locus_tag="ACSIGJ_001061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329939.1"
                     /translation="MDKGTVLSDGISFLNIVGLPRIPAEQLPLCRLKPVSVAFQIDIQ
                     TQ"
     gene            10766..14941
                     /gene="rpoC"
                     /locus_tag="ACSIGJ_001062"
     CDS             10766..14941
                     /gene="rpoC"
                     /locus_tag="ACSIGJ_001062"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HHQ1329940.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLNMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLDVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENMEEGVTVAKQTDDVTGLSALVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDMMAANEKALEEGKEPARYENILLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            15230..15643
                     /locus_tag="ACSIGJ_001063"
     CDS             15230..15643
                     /locus_tag="ACSIGJ_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329941.1"
                     /translation="MKVSQSAMNKLVIAVIASMGLTACAGGGSDSSMSVQPSVSEQLK
                     DNANVDAKDEKVIEYLKKSSLKDVPKELQAKVLKVKGDEYTGVRKQYAGKLGKGESVK
                     AMLFLDGEEPFSKEQLQKMDVYVNGKKYEGSKGGN"
     gene            15661..16290
                     /locus_tag="ACSIGJ_001064"
     CDS             15661..16290
                     /locus_tag="ACSIGJ_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding protein domain-containing
                     protein"
                     /protein_id="HHQ1329942.1"
                     /translation="MSEQKIEFYGADKEQNYALLKTWVYEQPYSVVRGYFGYSRKDGN
                     PIEEDGQNPEEIPFDLYLGDIRGVATDEDKLPKAGSFQYEGRAFGGNGVLSKESLDNH
                     NGVFRYTIDFDRRKGSGSIEGMEQYGKIKLEEAAIERIPYRESGSSLGLKDRVSYFGV
                     NEGVAMLEKDNEIKKYHLGIFGEAANEVAGAVSQEHKHQAVIGFGGEKK"
     gene            16530..16772
                     /locus_tag="ACSIGJ_001065"
     CDS             16530..16772
                     /locus_tag="ACSIGJ_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329943.1"
                     /translation="MKSKNIFFMGYLIASATFAEDIGVPVEPINVGSRAAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGGRSIP"
     gene            16969..17829
                     /locus_tag="ACSIGJ_001066"
                     /pseudo
     CDS             16969..17829
                     /locus_tag="ACSIGJ_001066"
                     /inference="COORDINATES: protein motif:HMM:NF016462.6"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
     gene            18008..18379
                     /gene="rpsL"
                     /locus_tag="ACSIGJ_001067"
     CDS             18008..18379
                     /gene="rpsL"
                     /locus_tag="ACSIGJ_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HHQ1329944.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            18497..18967
                     /gene="rpsG"
                     /locus_tag="ACSIGJ_001068"
     CDS             18497..18967
                     /gene="rpsG"
                     /locus_tag="ACSIGJ_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HHQ1329945.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKAAIEVFNEAIANSKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            18986..21091
                     /gene="fusA"
                     /locus_tag="ACSIGJ_001069"
     CDS             18986..21091
                     /gene="fusA"
                     /locus_tag="ACSIGJ_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HHQ1329946.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDSFTGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGEDLAEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            21179..22363
                     /gene="tuf"
                     /locus_tag="ACSIGJ_001070"
     CDS             21179..22363
                     /gene="tuf"
                     /locus_tag="ACSIGJ_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HHQ1329947.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSS
                     YDFPGDDCPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVG
                     VLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFR
                     TTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSV
                     IA"
     gene            22381..22692
                     /gene="rpsJ"
                     /locus_tag="ACSIGJ_001071"
     CDS             22381..22692
                     /gene="rpsJ"
                     /locus_tag="ACSIGJ_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HHQ1329948.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
BASE COUNT         6159 a         5071 c         5879 g         5639 t
ORIGIN      
        1 ttgtcgggat tcgctcaaat cgggcggcgg cgggatggtg ttgcagatta acgatgtgga
       61 taccaacagc ggggaatttg caatctatac cgctcaggat gcatcggtaa agctggattt
      121 gttaaaaatg gaattaaaac actgcaaaga aatgttggaa caaaaagaca aagaaatcga
      181 gttgctccgc aagctgaccg aaaccgttta aacagatatg ccgtctgaaa aaagttttca
      241 ggcggcatat tcttcgacag gtcttgtata ataccgtttg aactttcagg cttttgatta
      301 tggcggcagg caaacatacc aaacacagca accgggtacg cattatcggc gggcaatgcc
      361 ggggcaggaa attgagtttt gcatccgccg acggactgcg cccaaccccc gacagcgtgc
      421 gtgaaaagct gtttaactgg ctgggacagg atttgacggg aaaaacggtt ttggatctct
      481 tcggaggcag cggcgcactc ggcatggagg cggcatcgcg taatgcaaaa cgggtcgtga
      541 ttgcagacaa caaccgtcaa acggtgcaaa cccttgagaa aaacagccgc gaactgggtt
      601 tggggcaggt gcaaaccgtc tgttcggacg gcatcgcata tttggcaaac ctaaaagaga
      661 aattcgatgt cgtcttcctt gatccgccgt ttgcgtggca aagttgggaa agtcttttca
      721 atgtattggg cacacgcctg aatgacggcg catatgttta tatcgaggca ggcaggcagc
      781 cggacaaacc tgattggctg acgggatata gggaggggaa gtcggggcag ggtacatttg
      841 aattaagggt tttccaagtg gctgaataat atgcgctttg ataatcattt ccgggttgta
      901 aacattcgtt tgcaaccgtc cggttcaaaa aaaccttgtg ctataatccg cgcccgcccg
      961 gttttgataa tttagtggaa aaggaaaaga aatgtcgctt tttattaccg acgagtgcat
     1021 caactgcgac gtatgcgaac ccgaatgccc caatgatgcc atttcccaag gcgaggaaat
     1081 ttacgaaatc aaccccaacc tctgcacgca gtgcgtcgga cactacgatg agccgcagtg
     1141 ccagcaggtt tgcccggtgg actgcatcct gattgacgaa gaacatcccg aaacccatga
     1201 cgagttgatg gcgaaatacg aaaagattat ccagtttaaa taaattcttt ttaaaacatc
     1261 aaattatgtc tgttttgaaa taaaatcaaa aaaaaacttg acggaaaagc aagccgctaa
     1321 taaactagtg ttctcttttg gagggattcc cgagcggtca aagggggcag actgtaaatc
     1381 tgttgcgaaa gcttcgaagg ttcgaatcct tctccctcca ccaaaattct tacttggggc
     1441 agtagcgagt aatgcgggtg tagctcaatg gtagagcaga agccttccaa gcttacggtg
     1501 agggttcgat tcccttcacc cgctccaaac aattaggccc atgtagctca ggggtagagc
     1561 actcccttgg taagggagag gtcggcagtt caaatctgcc catgggcacc atctctcgat
     1621 tattcatttc tttaaggctt agatatatag gatattgcca tggctaagga aaaattcgaa
     1681 cgtagcaaac cgcacgtaaa cgttggcacc atcggtcacg ttgaccatgg taaaaccacc
     1741 ctgactgctg ctttgactac tattttagct aaaaaattcg gcggcgctgc aaaagcttac
     1801 gaccaaatcg acaacgcacc cgaagaaaaa gcacgcggta ttaccattaa cacctcgcac
     1861 gtagaatacg aaaccgaaac ccgccactac gcacacgtag actgtccggg tcacgccgac
     1921 tacgttaaaa acatgattac cggcgccgca caaatggacg gtgcaatcct ggtatgttct
     1981 gctgccgacg gccctatgcc gcaaacccgc gaacacatcc tgctggcccg tcaagtaggc
     2041 gtaccttaca tcatcgtgtt catgaacaaa tgcgacatgg tcgacgatgc cgagctgttg
     2101 gaactggttg aaatggaaat ccgcgacctg ctgtccagct acgacttccc cggcgacgac
     2161 tgcccgatcg tacaaggttc cgcactgaaa gccttggaag gcgatgccgc ttacgaagaa
     2221 aaaatcttcg aactggctac cgcattggac agctacatcc cgactcccga gcgtgccgtg
     2281 gacaaaccat tcctgctgcc tatcgaagac gtgttctcca tttccggccg cggtaccgta
     2341 gtcaccggcc gtgtagagcg aggtatcatc cacgttggtg acgagattga aatcgtcggt
     2401 ctgaaagaaa cccaaaaaac cacctgtacc ggcgttgaaa tgttccgcaa actgctggac
     2461 gaaggtcagg cgggcgacaa cgtaggcgta ttgctgcgcg gtaccaaacg tgaagacgta
     2521 gaacgcggtc aggtattggc caaaccgggt actatcactc ctcacaccaa gttcaaagca
     2581 gaagtgtacg tattgagcaa agaagagggc ggccgccata ccccgttttt cgccaactac
     2641 cgtccccaat tctacttccg taccactgac gtaaccggcg cggttacttt ggaaaaaggt
     2701 gtggaaatgg taatgccggg tgagaacgta accattactg tagaactgat tgcgcctatc
     2761 gctatggaag aaggtctgcg ctttgcgatt cgcgaaggcg gccgtaccgt gggtgccggc
     2821 gtggtttctt ctgttatcgc ttaagtttag aggccaatag ctcaattggt agagtatcgg
     2881 tctccaaaac cgagggttgg gggttcgaga ccctcttggc ctgccaaata aaaaattaac
     2941 cggccttgtg ccggttaatt tttttgtatt tgttatttag taaactctct tgccatttac
     3001 atggattgag aatagacaga tgctatgatg gataaataat atgacagaac atacgcctga
     3061 aaaaaagaat gttaaagtga atcaactggt tgttcaagat aaagaatctg catctaattc
     3121 cggtaaggag ggtttttttg catatttctc aaattcttgg tccgaattca aaaaggtggt
     3181 ttggcctaag cgtgaagatg ccgtcagaat gactgtattt gttatagtgt ttgttgctgt
     3241 gctttctata tttatctatg cggcagatac agcaatttcg tggttatttt ttgatgtatt
     3301 gctgagaagg gaaggttgag atgtcgaaaa aatggtatgt tgtacaggcg tattcggggt
     3361 ttgagaagaa tgtccaacga atattggaag agcgaattgc ccgtgaggag atgggagatt
     3421 atttcggaca aattctggtg cctgtagaga aagttgttga tatccgcaat ggtcgtaaga
     3481 ctattagtga aagaaagtca tatcctggtt atgtgttagt tgagatggaa atgacagatg
     3541 actcttggca tcttgtaaaa agcactcccc gtgtttctgg ttttattgga gggagggcta
     3601 atagacccac gccgattagt cagagagagg ctgaaattat tttacagcag gttcagaccg
     3661 gcatagagaa gccgaaacca aaagttgaat ttgaggttgg tcaacaggtt cgtgtaaatg
     3721 aaggaccgtt tgcggatttt aacggggtgg ttgaggaggt caattatgaa cggaataagt
     3781 tacgcgtgtc tgttcagata tttggtagag aaacacccgt tgagctggag ttcagccagg
     3841 ttgagaagat taactgattt ttatacttga aaaaaaagca ataagaggat agaatcaaaa
     3901 attaacttgg ggagcggaaa tggttccgcg tcttacccgt ttttaggagt ccgttaagtg
     3961 gcaaagaaaa ttatcggcta tattaaactg caaattcctg caggcaaagc caatccatcc
     4021 cccccagttg gtcctgcttt gggtcaacgt ggtttgaata tcatggaatt ctgtaaagca
     4081 tttaatgctg caacccaagg tatggaatct ggtttgccaa ttccggttgt gattactgca
     4141 tttgcagata aatcattcac atttgtgatg aaaactcctc cggcttctat tctgttgaaa
     4201 aaagccgccg gtttgcaaaa aggtagttct aatcctctga caaacaaggt aggtaaattg
     4261 acccgtgccc agttggaaga aattgctaaa actaaagagc ctgatttgac tgctgctgac
     4321 ttggatgcgg ctgttcgtac tatagcaggt tctgctcgct caatgggctt ggacgtggag
     4381 ggtgttgtat aatggctaaa gtatctaaac gcttgaaagt tcttcgctct tctgttgaag
     4441 ctaacaaact gtacgcaatc gacgaagcaa ttgccttggt taaaaaagct gctaccgcta
     4501 aatttgacga atctgttgac gtatctttca acttgggcgt tgattctcgt aaatctgacc
     4561 aagttatccg tggttcagtc gttctgccta aaggtactgg taagacaacc cgtgtggctg
     4621 tatttactca aggtgtaaat gcagaagctg ctaaagaggc tggtgcggat gtcgtcggtt
     4681 tcgaagattt ggctgctgaa atcaaagcag gcaatctgaa ctttgatgtt gttattgctt
     4741 ctcctgatgc aatgcgtatc gttggtcaat tgggtatcat cttgggtcct cgtggtctga
     4801 tgccaaaccc taaaataggt acggttaccc ctaatgttgc cgaagcggta aaaaatgcaa
     4861 aagcaggtca agtgcagtac cgtaccgaca aggcaggtat tgttcatgca acgattggtc
     4921 gtgcttcgtt cgctgaagct gatttgaaag agaactttga tgcgttgctg gacgctatcg
     4981 ttaaagccaa acctgctgct gctaaaggtc agtacctgaa aaaagttgct gtatccagca
     5041 ctatgggttt aggtgttcgc gttgatacat caagcgtgaa taactaatct aagaaatttt
     5101 caagcaactc tgtttttgag ttgcttgaat ttgggctact taaaattaag tagatgtcca
     5161 agaccgtagg gatcgtaaga tttaatcgta actgccctac gcagacggta gtcctgaaac
     5221 acattgcaag attgcttgta agatgtcttt ttaggttacc gcgctggtgg gatatcgttt
     5281 tggtatcctg tttataaaca gtgggaggta gaccttgagt ctcaatattg aaaccaagaa
     5341 agtggcggtc gaggaaatta gcgcggcaat tgctaatgct caaaccctcg tagtcgctga
     5401 atatcgcggt atcagtgttt ccagtatgac tgagcttcgt gcgaatgcac gtaaagaagg
     5461 cgtttatttg cgcgttctga aaaatactct ggctcgtcgc gcagtgcaag gtacttcatt
     5521 tgtagaattg gccgatcaaa tggttggtcc gttggtttac gctgcttctg aagatgctgt
     5581 tgctgctgct aaagtgttgc accaattcgc gaaaaaagat gacaaaattg tcgttaaagc
     5641 cggttcttac aatggcgaag taatgaatgc tgctcaggtt gctgagttgg cttctattcc
     5701 gagccgcgaa gagctgttgt ccaaactgtt gttcgttatg caagctcctg tatcgggctt
     5761 tgcgcgcggt ttggctgctt tggcagagaa aaaagccggc gaagaagctg cttaatcgat
     5821 tttgtttctg ttaatcaatt attttttaat acaatatttg gagtaaaata gcatggctat
     5881 tactaaagaa gacattttgg aagcagttgg ttctttgacc gtaatggaat tgaatgacct
     5941 ggttaaagct tttgaagaaa aattcggtgt ttctgctgct gctgttgcag ttgcaggtcc
     6001 tgctggtgcc ggtgctgccg atgctgaaga aaaaaccgaa tttgatgtcg ttttggcttc
     6061 tgccggcgat caaaaagtcg gcgtgattaa agttgtccgt gcaattactg gtttgggtct
     6121 gaaagaagct aaagacatcg ttgacggcgc acctaaaacc attaaagagg gtgtttctaa
     6181 agctgaagcc gaagacatcc aaaaacaact ggaagcagca ggcgctaaag tcgaaatcaa
     6241 ataatttgat gcttcttatg aaggctggca gttttctgcc agccttattt tgcttcttaa
     6301 aataagcatt aagtattgtt tacatttatt tgcatagttt ttatcaaatc attgcaaata
     6361 aatgtaaata tcagattgat gcgtaccgtt gttttcagac ggcctattat tgaaaattac
     6421 ttttcggagt gtgtatgaac tattcgttta ccgagaaaaa acgtatccgt aagagttttg
     6481 caaagcggga aaatgttttg gaagttcctt ttctactggc aacccaaatt gattcttatg
     6541 cgaaattttt gcagctggaa aatgcttttg acaaacgtac cgatgacggt ctgcaagcgg
     6601 catttaattc tattttcccg attgtgagcc acaacggtta tgctcgtctg gagtttgtat
     6661 attacacgct gggcgagcct ttgttcgata tccctgaatg tcaattgcgc ggaatcactt
     6721 atgctgcccc tctgcgcgcg cgtatccgtt tggtgatttt ggataaggaa gcgtctaaac
     6781 cgacggtaaa agaagttcgt gaaaacgaag tgtatatggg cgaaattccg ttgatgaccc
     6841 cgagcggttc ttttgtgatt aacggcacag agcgtgtgat tgtttcccag ttgcaccgtt
     6901 cgcccggtgt gttcttcgag catgacaaag gtaagacaca ctcttccggc aaattgttat
     6961 tctccgcccg catcattccc taccgtggtt catggttgga ttttgaattt gatccgaaag
     7021 atttgctgta tttccgtatc gaccgccgcc gtaaaatgcc ggtaacgatt ttgttgaagg
     7081 ctttgggcta caacaatgag caaatcttgg atattttcta cgacaaagaa acgttttatt
     7141 tgtcttcaaa cggtgttcaa accgatttgg tcgcaggccg tctgaaaggc gaaactgcca
     7201 aggtcgatat cttggataaa gaaggcaatg tattggttgc caaaggtaag cgcattactg
     7261 cgaaaaatat ccgtgatatt accaatgcag gtctgacccg tttggatgta gaacaggaaa
     7321 gcctgctggg caaagcattg gctgccgatt tgattgattc ggaaaccggc gaggtattgg
     7381 cttctgccaa tgatgaaatt acagaagagt tgttggccaa atttgatatt aacggcgtaa
     7441 aagaaattac gaccctttac atcaatgagt tggatcaggg tgcttatatt tccaatacct
     7501 tgcgcacgga tgagactgcc ggccggcagg cggcgcgtgt tgcgatttac cgtatgatgc
     7561 gtccgggcga accgcccacc gaagaggcgg tcgagcaatt gtttaaccgc ttgttcttca
     7621 gtgaagacag ctacgatttg tcccgcgtag gccgtatgaa atttaatacg cgcacatacg
     7681 aacaaaaact gtccgaagcc caacaaaact cttggtacgg ccgcctgttg aacgagacgt
     7741 ttgccggtgc tgccgacaaa ggcggttatg tcctgagcgt cgaagatatt gtcgcctcga
     7801 ttgcgacttt ggtcgagttg cgtaacggcc atggcgaagt ggacgatatc gatcacttgg
     7861 gcaaccgtcg agtacggtcg gtaggcgagc tgactgaaaa ccaattccgc agcggtttgg
     7921 ctcgtgtgga acgtgccgta aaagaacgtt tgaatcaggc ggaatcagaa aacttgatgc
     7981 cgcacgactt gattaatgcg aaacccgttt ctgccgccat caaagaattc ttcggctcca
     8041 gccaattgag tcagtttatg gatcagacca atcccttgtc tgaagtaacc aataaacgcc
     8101 gtgtatctgc attgggcccg ggcggtttga cccgcgaacg tgccggcttc gaagtgcggg
     8161 acgtgcatcc gacccactat ggacgtgtat gtcctattga aacgcctgaa ggtccgaaca
     8221 tcggtttgat caactcattg tctgtttacg cgcgcaccaa tgattacggt ttcttggaaa
     8281 cgccttaccg ccgcgttatt gacggcaaag taaccgagga aattgattac ttgtctgcca
     8341 tcgaagaagg ccgctatgtg attgcacagg cgaatgccga tttggattca gacggcaatc
     8401 tgattggcga tttggttacc tgtcgtgaaa aaggcgaaac cattatggca acgcccgacc
     8461 gcgtccaata tatggacgtg gcaaccggtc aggtggtatc cgttgcggca tccctgattc
     8521 cgttcttgga acacgatgac gcgaaccgcg cattgatggg tgccaacatg caacgtcagg
     8581 cagtgccttg tctgcgtcct gaaaaaccga tggtcggtac cggtatcgag cgttccgttg
     8641 ccgttgactc tgctactgca atcgttgccc gccgaggcgg cgtggtcgag tatgtcgatg
     8701 ccaaccgcgt tgtgatccgt gtccatgacg acgaagcgac tgccggcgaa gtgggtgtcg
     8761 atatttacaa tttggttaaa ttcacccgtt ccaatcaatc taccaacatc aaccaacgtc
     8821 cggctgtcaa agcaggcgat gttttgcaac gcggcgattt ggtggccgac ggcgcgtcca
     8881 ccgatttggg cgaattggct ttgggtcaaa atatgaccat cgccttcatg ccgtggaacg
     8941 gttacaacta cgaagactcg attctgattt ccgaaaaagt ggctgcggac gaccgctata
     9001 cttcgattca cattgaggaa ttgaatgtcg ttgcccgcga taccaagctg ggtgcggaag
     9061 acattacccg cgatattccg aacttgtccg agcgtatgca aaaccgtttg gacgaatccg
     9121 gtatcgttta catcggtgcg gaagtagaag ccggcgatgt gttggtaggc aaggtaacgc
     9181 ctaaaggcga aacccagctg acgccggaag aaaaactgct gcgcgccatc ttcggcgaaa
     9241 aagcgtctga cgtaaaagat acttcattgc gtatgcctac cggtatgagc ggtacggtta
     9301 tcgacgttca agtctttacc cgcgaaggca tccaacgcga caaacgcgcc caatccatta
     9361 tcgattccga attgaaacgc taccgtttgg atttgaacga ccaattgcgt attttcgaca
     9421 acgacgcatt cgaccgtatc gagcgtatga ttgtcggtca gaaagccaac ggcggtccga
     9481 tgaagctggc caaaggcagc gaaatcacga ccgaatatct ggcgggtctg ccgagcaggc
     9541 acgattggtt tgatatccgt ttgaccgatg aagatttggc caagcagttg gaactgatta
     9601 aattgagcct gcaacaaaaa cgcgaagaag cggatgagtt gtatgagatc aagaagaaaa
     9661 aactgaccca aggcgacgag ctgcaacccg gcgtacaaaa aatggtgaaa gtctttatcg
     9721 ccatcaaacg ccgtctgcaa gccggtgaca aaatggcggg ccgccacggt aataaaggtg
     9781 tggtatctcg cattctgcct gtggaagaca tgccgtacat ggcggacggc cgtcctgtgg
     9841 acatcgtact gaacccgttg ggcgtacctt cccgtatgaa catcggtcag attttggaag
     9901 ttcacttggg ttgggcggca aaaggtatcg gcgagcgcat cgaccgtatg ctgaaagagc
     9961 gacgcaaagc cggcgagctg cgcgagttct tgaacaaact ctacaacggc agcggtaaga
    10021 aagaagattt ggacagcctg actgatgaag aaatcatcga attggcttcc aatctgcgta
    10081 aaggtgcatc tttcgcctct cctgtattcg acggtgcgaa agagtctgaa atccgcgaaa
    10141 tgttgaactt ggcttaccca agcgaagatc ctgaggttga gaaactgggc ttcaacgaca
    10201 gtaaaactca aatcacgctg tatgacggcc gttcaggcga agcatttgac cgcaaggtta
    10261 cagtcggtgt gatgcactat ctgaaactgc accacttggt tgacgaaaaa atgcacgcgc
    10321 gttctaccgg cccgtacagt ctggttactc aacaacctct gggcggtaaa gctcagtttg
    10381 gcggccaacg tttcggtgag atggaggttt gggcattgga agcatacggc gcggcataca
    10441 cgctgcaaga gatgctgact gtgaagtctg acgacgtgaa cggccgtacc aaaatgtacg
    10501 aaaacatcgt caaaggcgaa cacaaaatcg atgccggtat gcccgagtcc ttcaacgtat
    10561 tggtcaaaga gattcgctca ctgggtttgg atatcgattt ggaacgctac tgatacgggt
    10621 ttcagacggc ataaggggag ctgttctgca ggtatgcggg gcagcccgac aatgtttaaa
    10681 aacgaaatgc cgtctgaaag cactgtacct ttatccatat cgaaaatccg ccacgcggta
    10741 aaaatatttc cttcaaggag cagaaatgaa tttgttgaac ttatttaatc cgttgcaaac
    10801 cgcaggtatg gaagaagagt ttgatgccat caaaatcggt attgcctctc ccgaaaccat
    10861 ccgctcatgg tcttatggcg aagttaaaaa acctgaaacc atcaactacc gtacgttcaa
    10921 acccgagcgc gacggcttgt tctgcgccaa aatctttggc ccggtcaaag actacgaatg
    10981 cttgtgcgga aaatacaaac gattgaaatt taaaggcgta acctgtgaaa aatgtggcgt
    11041 agaagttacc ctgtccaaag tgcgccgcga acgcatgggc catatcgaat tggctgcacc
    11101 cgttgcccat atttggttct tgaaatccct gccttcccgc ttgggtatgg tattgaacat
    11161 gacgttgcgt gatatcgaac gcgtattgta ctttgaagca ttcgtggtga ccgatcccgg
    11221 catgactccg ttgcaacgtc gtcaattgct gactgaagac gattactaca acaaactgga
    11281 cgaatacggc gacgacttcg atgccaaaat gggtgcggaa ggtatccgcg aattgttgcg
    11341 aaccttggat gtagcaggcg aaatcgaaat cctgcgccaa gagcttgaat cgaccggttc
    11401 tgacaccaaa atcaaaaaaa tcgctaaacg cttgaaagta ttggaagcct tccatcgttc
    11461 cggtatgaag ctggagtgga tgattatgga tgtgctgccg gtattgccgc ctgatttgcg
    11521 tccgttggtt ccgttggacg gcggccgttt tgccacttcc gatttgaacg atttgtaccg
    11581 ccgcgtcatc aaccgcaaca accgtctgaa acgcctgctg gaactgcatg cgcctgacat
    11641 catcgtccgc aacgaaaaac gtatgttgca agaagcggtt gactcgctgt tggataacgg
    11701 ccgtcgcggt aaagccatga ccggagccaa caaacgtccg ctgaaatcgt tggcagatat
    11761 gattaaaggt aaaggcggcc gcttccgtca aaacctgttg ggcaaacgtg tggactactc
    11821 cggccgttcc gtgattaccg taggcccata cctgcgtctg caccaatgcg gtttgccgaa
    11881 aaaaatggcg ttggaactgt tcaaaccatt tattttccac aaattggaaa aacaaggttt
    11941 ggcgtctacc gttaaagctg cgaaaaaatt ggtagaacaa gaagtacctg aagtatggga
    12001 tatcttggaa gaagtcatcc gcgaacatcc gattatgttg aaccgtgcgc cgaccctgca
    12061 ccgtttgggt attcaagcgt tcgagcctat tctgatcgaa ggtaaagcga ttcagttgca
    12121 cccattggtg tgtgccgcat ttaatgccga ctttgacggt gaccaaatgg cagtacacgt
    12181 tccattgagc ttggaagcgc aaatggaagc acgcacgctg atgctggctt caaacaacgt
    12241 attgtctcct gccaacggcg aaccgattat cgtaccttcc caagacatcg tattgggtct
    12301 gtactacatg acccgcgacc gtatcaatgc caaaggcgag ggcagcctgt ttgccgatgt
    12361 gaaagaagtg catcgtgcat accataccaa acaggtcgag ctgggtacaa aaataaccgt
    12421 acgtctgcgc gaatgggtga aaaacgaagc aggtgagttt gagcctgtcg ttaaccgtta
    12481 cgaaacaacc gtcggccgtg cattgttgag cgaaatcctg ccgaaaggcc tgccgtttga
    12541 atatgtcaac aaagcgttga agaaaaaaga aatttccaag ctgattaacg catcgttccg
    12601 cctgtgcggc ttgcgcgata cggttatctt cgccgaccac ctgatgtaca ccggtttcgg
    12661 atttgcggca aaaggcggta tttccattgc cgttgacgat atggaaattc caaaagaaaa
    12721 agcggccttg ctggctgaag ccaatgccga agttaaagaa atcgaagacc aataccgtca
    12781 aggtttggtt accaacggcg aacgctacaa caaagtggtg gatatttggg gtcgtgccgg
    12841 cgataagatt gctaaagcga tgatggacaa cctgtccaaa caaaaagtta tcgaccgtga
    12901 cggcaacgaa gtcgatcaag agtcattcaa ctccatttat atgatggcgg actccggtgc
    12961 ccgtggttct gcggctcaga ttaaacagtt gtccggtatg cgcggtttga tggcgaaacc
    13021 tgacggatcg attattgaaa cgccgattac ctcaaacttc cgcgaaggtc tgaccgtatt
    13081 gcaatacttt attgcgaccc acggtgcgcg taagggtttg gcggataccg cgttgaaaac
    13141 cgcgaactcc ggttacctga cccgtcgtct ggtagacgta actcaagact tggtcgttgt
    13201 tgaagacgat tgcggtactt cagacggctt tgtcatgaag gcagtggtac aaggcggtga
    13261 tgtgattgaa gcattgcgcg atcgtatttt gggtcgtgtt accgcgtctg acgttgtcga
    13321 tccgtcaagc ggcgagactt tggttgaagc cggtacgttg ttgactgaaa aactggtgga
    13381 tatgattgac caatccggtg tcgatgaagt caaagtccgt actccgatta cttgtaaaac
    13441 ccgccacggt ctgtgcgcgc actgttacgg ccgcgacttg gcgcgcggca aactggtcaa
    13501 tgccggcgag gcagtcggcg tgattgcggc tcagtctatt ggtgaaccgg gtacccagtt
    13561 gaccatgcgt acgttccaca tcggtggtgc ggcatcccgt gcggcagcag ccagccaagt
    13621 cgaagccaaa tccaacggta cggcacgttt cagcagccaa atgcgttatg ttgccaacaa
    13681 caaaggcgag ttggttgtca tcggccgttc ttgcgaagtc gtgattcacg atgacatcgg
    13741 ccgtgaacgc gaacgccaca aagtacctta cggtgccatc ctgctggtac aagacggtat
    13801 ggccattaaa gccggtcaaa ccttggcaac ttgggatccg catacccgtc cgatgattac
    13861 cgaacatgca ggtatggtga aattcgagaa catggaagag ggcgttaccg ttgcaaaaca
    13921 aaccgatgat gtaaccggtt tgtccgcttt ggtagtgatt gacggtaaac gccgttcctc
    13981 tagtgcttcc aaactgctgc gtccgactgt gaaactcttg gacgaaaacg gcgtggaaat
    14041 ctgtattccc ggtacgtcca ctccggtttc gatggcattc cccgttggtg cggtgattac
    14101 cgtacgcgaa ggtcaggaaa tcggtaaagg cgacgtattg gcgcgtattc cgcaagcctc
    14161 ttccaaaacc cgcgacatta ccggcggtct gccgcgcgtt gccgagctgt ttgaagcacg
    14221 cgtgccgaaa gatgccggta tgctggcgga aattaccggt accgtttcct tcggtaagga
    14281 gaccaaaggc aaacaacgtc tgattatcac tgacgtggac ggtgtagcat acgagacttt
    14341 gatttccaaa gagaaacaaa ttctggtaca tgacggtcaa gtggtaaacc gaggcgaaac
    14401 cattgtggac ggcgcggtcg atccgcacga tattctgcgt ctgcaaggta tcgaagcact
    14461 ggcacgctac attgtccaag aggtgcaaga ggtttaccgc ctgcaaggtg tgaagatttc
    14521 cgataaacac atcgaagtca tcatccgtca aatgttgcgc cgtgtgaaca ttgcggatgc
    14581 cggcgaaacc gggtttatta ccggagagca ggtcgaacgc ggcgatatga tggcggccaa
    14641 tgaaaaagct ttggaagaag gcaaagagcc ggcacgttac gaaaacatat tgctgggtat
    14701 taccaaagcc tccctgtcca ccgacagctt catttctgcc gcatcgttcc aagaaaccac
    14761 ccgtgtgttg accgaagctg ccatcatggg caaacaagac gaactgcgcg gtctgaaaga
    14821 gaatgtcatc gtcggtcgcc tgattcctgc cggtaccggt ttgacttatc accgcagccg
    14881 tcatcagcaa tggcaagggg tagagcagga gactgctgaa acccaagtaa cggatgaata
    14941 atcttcggcg cgttcattca ataaaaaacc gcaagccttg agcttgcggt ttttctttgt
    15001 ccgattaagg caaaaacaag cgttttcgtc attttgaggc gtgtggatat aagcccgata
    15061 agtgtcccat atcttcgtgc cgtcaaatta tgcatcgaat agaacgagtt aaatccgaag
    15121 gttattgtgt taaatattgc aaaaatactt taaaaatttc gcacggtggg agggtagaat
    15181 tcccagactt aaagcttaag tttttttaac aaagtttggg ggagtctaaa tgaaagtatc
    15241 tcaatcagca atgaataaac ttgttattgc tgttattgct tctatggggc taacagcgtg
    15301 cgcgggtggc ggttccgatt catctatgtc ggttcaacca agcgtttcgg aacaactgaa
    15361 agacaatgca aatgttgatg cgaaagatga aaaagttatt gaatatctga agaaatcatc
    15421 attaaaagat gtaccaaaag aattgcaagc caaagtatta aaggttaaag gtgatgaata
    15481 tacaggagtg cgaaaacaat atgcaggtaa gctcggtaag ggtgaatctg tcaaggcaat
    15541 gctatttcta gacggcgaag agccgtttag taaggaacaa cttcagaaga tggatgtata
    15601 tgttaatggt aaaaaatacg aaggtagtaa gggggggaac tagatgttct ccccaaaggt
    15661 ttgagtgagc aaaagattga gttttacggt gcggataaag aacagaatta tgcgctatta
    15721 aaaacttggg tttatgagca accgtattct gtggttagag gctattttgg ctacagtcga
    15781 aaagacggta atcctataga agaggatgga caaaatcctg aagagattcc ttttgacttg
    15841 tatctgggcg acattagagg cgtggcaaca gatgaagaca aattacccaa ggctgggagc
    15901 tttcaatatg aaggtcgggc atttggcggt aatggggttt tatccaaaga atcgttagat
    15961 aaccataatg gcgtatttag atacacaatt gattttgatc gtagaaaagg ctctggctca
    16021 attgaaggaa tggaacaata tggcaagatc aaattagaag aagctgctat tgagagaatc
    16081 ccttatcgag agtccggctc cagcttgggg ttgaaagacc gggtttctta tttcggcgtg
    16141 aacgaagggg tggctatgtt agagaaggat aatgagatta aaaagtatca tttgggtatt
    16201 ttcggcgaag cggctaatga agtagcaggt gctgtaagcc aagagcataa acatcaagct
    16261 gttatcggtt tcggcggcga gaagaaatag agaaagcact atttctattt agtgccgttt
    16321 gtccgggcaa gatgccgtct gaagtttcag acggcatttt cttcaaaaaa cagtccgcca
    16381 aatcgaatac tgccaagcgg caatgcaata cctcaaaaca gcaataattt cttttaaaac
    16441 agttgattct tactgaacat aaatggccgg gcaatacgaa aagaaaaatg gcgttgctgg
    16501 gcattttatt tgaatttgtt atgaaattga tgaaatctaa aaatatcttt tttatgggat
    16561 acctaatcgc ttcggcgacg tttgccgagg atatcggcgt gcctgtcgaa ccgattaacg
    16621 tcggtagtcg ggctgcgatg ccgtctgaag gggaaagcct cgccctcctg ccgtttgcag
    16681 aggatgtgcc gccggttcgc gatgcaatgc cgtctgaagt tcctaaaagc gcggcaggcg
    16741 gcgatgttcg aggcggccga tctataccgt gagttgttgt cggaaagacc cgatcttgtt
    16801 tatccccgtt ttgatttggg cgtgatgctg tttgaagaca aacaatatcg tgaggcattg
    16861 gttcagttgc accgggccga ggaggtgttg ccgcccgata tgcggcagct tgcacgggaa
    16921 tatatccgtc aggcggaagc ggtgcaggcg tggcatcctt cgttcaatat gaactatgaa
    16981 caaacagata atgtaaacaa tgcgtccctt tcacgggaca tcgtcatcaa cggccggaaa
    17041 tggataaaat cggaagacag cctgcccaaa cgcgccaacg ggataaggta tgaattgggt
    17101 attgaccgga tgtttaacat ggcaggcaat cactttgccc gcttaggcat cagcggttcc
    17161 ggtgtgcatt attggaatgc ccgggatttt agcgaacagg cgtttcatgc cgaagtaggc
    17221 taccgttatc ggaatagccg tctagagtgg ggattccggc cctttgtcaa acaaaaccgg
    17281 ctgggcaata accgttatac ggcaaatacg ggtattgcgt tggattacag ccgccgtctg
    17341 aatgaaaaat ggcgcagcac ccaatctttc cagtatggcc gcaaacaata ccatgacgaa
    17401 tatttggcaa aacgttacaa cagtaaaacg atttcggtga gcggtacgtt cagctattac
    17461 gctatgtcgg cttggcagtt gtacgggggt attagcggta tgtttgataa tactgtggag
    17521 aaagagcagg cttcacggcg ttatggtgta agtttgggta cggtgaaaat attagacggc
    17581 ggtcttggac tgaagttggg tgcgggttac acgaaacgga tatttaaagc ccctgcaaca
    17641 ttaatttaca attttactcg gcgtgatgat taacatcgga tgagcgcggc tttatggcat
    17701 aaaaaactgt cgtggaaagg atttacaccc caaataaatt tccgttacaa caagaacaac
    17761 agcaatatgc ccgcctttta ttcgcacagc ggcaagggat ggtttgtcag tatggaaaaa
    17821 acgtattgac agtattttct ccagccgtcc gactgattgt gagggatgtc ggtaaatatt
    17881 tatcggcaaa caagaaaatc atcttttttc ttgtcgttat gcttgactgt ctgcttgcaa
    17941 taaaaatata attccactct tgccgacatg gtgtcggcaa gtatttaact caacaggacg
    18001 agaaaatatg ccaactatca accaattggt acgcaaaggc cgtcaaaagc ccgtgtacgt
    18061 aaacaaagtg cccgcactgg aagcctgccc gcaaaaacgc ggcgtgtgca cccgtgtata
    18121 cacgactacc cctaaaaaac ctaactctgc attgcgtaaa gtatgtaaag tccgcctgac
    18181 caacggtttt gaagtcattt catatatcgg cggtgaaggc cacaacctgc aagagcacag
    18241 cgtcgtactg attcgcggcg gccgtgtaaa agacttgccg ggtgtacgtt accacactgt
    18301 acgcggttct ttggatactg caggtgttaa agaccgcaaa caagcccgtt ctaaatacgg
    18361 tgctaagcgt cctaaataat taccgggact caaataggca cgtcggccgc ctaagctgaa
    18421 caacggccga gtaagtgaat actcaattgg gtattcatgg gaatagaccc gactgaatag
    18481 attaaaggaa attaaaatgc caagacgtag agaagtcccc aagcgcgacg tactgccaga
    18541 tcctaaattc ggtagcgtcg agttgaccaa attcatgaac gtattgatga ttgacggtaa
    18601 aaaatccgtt gccgagcgta tcgtttacgg tgcgttggaa cagattgaga aaaaaaccgg
    18661 caaagcagca atcgaagtat ttaacgaagc cattgcaaac tccaaaccta tcgtggaagt
    18721 gaaaagccgc cgtgtaggtg gtgcaaacta ccaagttcct gttgaagttc gtccttcacg
    18781 ccgtctggct ttggcaatgc gttgggttcg cgacgcggcc cgcaaacgtg gtgagaaatc
    18841 catggatctg cgtttggcag gcgagttgat tgatgcgtcc gaaggccgtg gcggtgcgtt
    18901 gaaaaaacgt gaagaagtac accgtatggc tgaagccaac aaagcattct ctcacttccg
    18961 tttctaattt tgaaaggcta ataaaatggc tcgtaagacc ccgatcagcc tgtaccgcaa
    19021 catcggtatt tccgcccata tcgatgcggg taaaaccacg acgacagaac gtattttgtt
    19081 ctataccggt ttgacccaca agctgggcga agtgcatgac ggtgcggcta ctaccgacta
    19141 catggaacaa gagcaagagc gcggtattac cattacctcc gctgccgtta cttcctactg
    19201 gtccggtatg gcgaaacaat tccccgagca ccgcttcaac atcatcgaca ccccggggca
    19261 cgttgacttt accgtagagg tagagcgttc tatgcgtgta ttggacggcg cggtaatggt
    19321 ttactgtgcg gtgggcggtg ttcaaccgca atctgaaacc gtatggcggc aagccaacaa
    19381 ataccaagtt ccgcgcttgg cgtttgtcaa taaaatggac cgccaaggtg ccaacttctt
    19441 ccgcgttgtc gagcaaatga aaacccgttt gcgcgcaaac cccgtaccta tcgtcattcc
    19501 ggtaggcgcg gaagacagtt tcaccggtgt tgtcgatttg ctgaaaatga aatctatcat
    19561 ctggaatgaa gccgataaag gtacaacctt tacctatggc gatattcctg ccgaattggt
    19621 cgaaactgcc gaagaatggc gtcaaaatat gattgaagcc gcagccgaag ccagcgaaga
    19681 actgatggac aaatacttgg gcggtgaaga tctggccgaa gaagaaatcg taggcgcgtt
    19741 gcgtcaacgt actttggcag gcgaaattca gcctatgctg tgcggttctg catttaaaaa
    19801 caaaggtgtt caacgtatgt tggacgcagt tgtagaattg ctgccagctc ctaccgatat
    19861 tcctccggtt caaggtgtta atcctaacac tgaagaagcc gacagccgtc aagccagcga
    19921 tgaagagaaa ttctctgcat tggcattcaa aatgttgaac gacaaatacg tcggtcagct
    19981 gacctttatc cgcgtttact caggcgtagt aaaatccggc gataccgtat tgaactccgt
    20041 aaaaggcact cgcgaacgta tcggtcgttt ggtgcaaatg actgccgcag accgtactga
    20101 aatcgaagaa gtacgcgccg gcgacattgc agccgctatc ggtctgaaag acgttactac
    20161 cggtgaaacc ctgtgtgcgg aaagcgcgcc gatcatcttg gaacgcatgg aattccccga
    20221 gccggtaatc catattgccg ttgagccgaa aaccaaagcc gaccaagaga aaatgggtat
    20281 cgccctgaac cgcttggcta aagaagaccc ttctttccgt gtccgtacag acgaagaatc
    20341 cggtcaaacc attatttccg gtatgggtga gctgcacttg gaaattattg ttgaccgtat
    20401 gaaacgcgaa ttcggtgtgg aagcaaatat cggtgcacct caagtggctt accgtgaaac
    20461 tatccgcaaa gccgttaaag ccgaatacaa acatgcaaaa caatccggtg gtaaaggtca
    20521 atacggtcac gttgtgattg aaatggaacc tatggaaccg ggtggtgaag gttacgagtt
    20581 tatcgatgaa attaaaggtg gtgtgattcc tcgcgaattc attccgtctg tcgataaagg
    20641 tatccgcgat acgttgccta acggtatcgt tgccggctat cctgtagttg acgtacgtat
    20701 ccgtctggta ttcggttctt accatgatgt cgactcttcc caattggcat ttgaattggc
    20761 tgcttctcaa gcgtttaaag aaggtatgcg tcaagcatct cctgccctgc ttgagccgat
    20821 tatggcagtt gaagtggaaa ctccggaaga atacatgggc gacgtaatgg gcgacttgaa
    20881 ccgccgtcgc ggtgttgtat tgggtatgga tgatgacggt atcggcggta aaaaagtccg
    20941 tgccgaagta cctctggcag aaatgttcgg ttattcgacc gacctgcgtt ctgcaaccca
    21001 aggccgcgct acttactcta tggagttcaa gaaatattct gaagctcctg ctcacatagc
    21061 tgctgctgta actgaagccc gtaaaggcta atcaggcaaa taggccgtcc gaaaggctga
    21121 aatgattttt cagacggcat tgttctttaa tcgatcttta atgtaaagga attagctcat
    21181 ggctaaggaa aaattcgaac gtagcaaacc gcacgtaaac gttggcacca tcggtcacgt
    21241 tgaccatggt aaaaccaccc tgactgccgc tttgactact attttggcta aaaaattcgg
    21301 cggtgctgca aaagcttacg accaaatcga caacgcaccc gaagaaaaag cacgcggtat
    21361 taccattaac acctcgcacg tggaatacga aaccgaaacc cgccactacg cacacgtaga
    21421 ctgcccgggg cacgccgact acgttaaaaa catgattacc ggcgccgcac aaatggacgg
    21481 tgcaatcctg gtatgttccg cagccgacgg ccctatgccg caaacccgcg aacacatcct
    21541 gctggcccgt caagtaggcg taccttacat catcgtgttc atgaacaaat gcgacatggt
    21601 cgacgatgcc gagctgttgg aactggttga aatggaaatc cgcgacctgc tgtccagcta
    21661 cgacttcccc ggcgatgact gcccgattgt acaaggttcc gcactgaaag ccttggaagg
    21721 cgatgccgct tatgaagaga aaatcttcga attggctgct gcattggaca gctacatccc
    21781 gactcccgag cgtgccgtgg acaaaccttt cttgttgcct atcgaagacg tgttctccat
    21841 ttccggccga ggtacagtag taaccggccg tgtagagcgc ggtatcatcc acgttggtga
    21901 cgagatcgaa atcgtcggtc tgaaagaaac ccaaaaaacc acttgtaccg gtgttgaaat
    21961 gttccgcaaa ctgctggacg aaggtcaggc gggcgacaac gtaggcgtat tgctgcgcgg
    22021 taccaaacgt gaagacgtag agcgtggtca agtattggct aaaccgggta caatcactcc
    22081 tcacaccaag ttcaaagcag aagtatacgt actgagcaaa gaagagggtg gccgtcacac
    22141 tccattcttc gccaactacc gtccgcaatt ctacttccgt accaccgacg taaccggcgc
    22201 ggttactttg gaagaaggtg tggaaatggt aatgccgggt gaaaacgtaa ccatcaccgt
    22261 agaactgatt gcgcctatcg ctatggaaga aggtttgcgc tttgcgattc gcgaaggcgg
    22321 ccgtaccgtg ggtgccggcg tggtttcttc tgttatcgct taattgaagg atattgataa
    22381 atggcaaacc aaaaaatccg tatccgcctg aaagcttatg attacgccct gattgaccgt
    22441 tctgcacaag aaatcgttga aactgcaaaa cgtactggtg cagttgtaaa aggcccgatt
    22501 cctttgccga ccaaaatcga gcgtttcaac attttgcgtt ctccgcacgt gaacaaaact
    22561 tcccgtgagc aattggaaat ccgcacccac ttgcgcctga tggacatcgt ggattggacc
    22621 gataaaacta ccgatgcgct gatgaagctg gatttgccgg ccggtgttga tgtagaaatc
    22681 aaagtccaat aattcggact attgaaaatc cccaagcaat caatgcttgg ggatttttta
    22741 tgttatgc
//
LOCUS       DBJGGI010000027        22497 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.027, whole genome shotgun sequence.
ACCESSION   DBJGGI010000027 DBJGGI010000000
VERSION     DBJGGI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22497
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.027"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(191..316)
                     /locus_tag="ACSIGJ_001072"
     CDS             complement(191..316)
                     /locus_tag="ACSIGJ_001072"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329949.1"
                     /translation="MPPETDFAKRRRVTGVEMETERVKPDVSVLRKPAIKGLAGV"
     gene            356..592
                     /locus_tag="ACSIGJ_001073"
     CDS             356..592
                     /locus_tag="ACSIGJ_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HHQ1329950.1"
                     /translation="MLRLQKWGNSAAVRLPARLLTALNAKIGDALETEIRNGELLIRP
                     VKRYRLSDLLAETEAAPPRAEGWEEMPDVGQEGV"
     gene            592..939
                     /locus_tag="ACSIGJ_001074"
     CDS             592..939
                     /locus_tag="ACSIGJ_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HHQ1329951.1"
                     /translation="MYIPERGDIFHLAFDPAAGTEMKGGHYAIALSPRAYNRATGLVY
                     ACPISQGRAAAARSGGMISTLLGAGTATQGNVHCHRMKALDWKIRRAAFRETVPDYVI
                     EDVLARIGAVLFD"
     gene            complement(994..1146)
                     /locus_tag="ACSIGJ_001075"
     CDS             complement(994..1146)
                     /locus_tag="ACSIGJ_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329952.1"
                     /translation="MKFSINREFRKMLKLFAEYAVLRRMFYAVLGAAPLTALIWKPAD
                     ILNALK"
     gene            complement(1305..1637)
                     /locus_tag="ACSIGJ_001076"
     CDS             complement(1305..1637)
                     /locus_tag="ACSIGJ_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329953.1"
                     /translation="MGFLQFGLLFAAAGGVLGAVWASLQERGRPVQAVLEAFISAIAA
                     AAAAERFVPLDQAWTCAAAGVFAGMMTGHALDTVRALAPKVLRGYLGGLAEKLTGVKD
                     GGSSDGKD"
     gene            complement(1638..2108)
                     /locus_tag="ACSIGJ_001077"
     CDS             complement(1638..2108)
                     /locus_tag="ACSIGJ_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329954.1"
                     /translation="MLRFLTERRFLPAGFQAWLFGTATRVLEAVSGLGLSGYAAVFAL
                     APDEIYAWRIYYKFQDIPEAWTVGVLAAAGLLQTALLFARGVRACVASAYLLLFSGFV
                     WFLVSVAFWGAYPPLNTGMVVPPLLAFFCALAGNNALRFLFSAQKSRGLADEGS"
     gene            complement(2179..2484)
                     /locus_tag="ACSIGJ_001078"
     CDS             complement(2179..2484)
                     /locus_tag="ACSIGJ_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329955.1"
                     /translation="MIEDKKIVALNFAVEDGMTGASAAYHVVEHIGADYRNGYVTATL
                     NGYVSENAFKSGKRYLLARTLDFQETDITAPDPGWVYRKALEGAAGIPKDAQPVYAE"
     gene            complement(2596..6741)
                     /locus_tag="ACSIGJ_001079"
     CDS             complement(2596..6741)
                     /locus_tag="ACSIGJ_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HHQ1329956.1"
                     /translation="MGGKSSTVTSAEERILSLQVQRSSQGLTLPVIYGRTRVAGNLIW
                     YGDFVTIEHKTTTRRGGKGGGGVKQVGISYTYEAAVMLALCEGEIQGVGRIWRDKEKF
                     DSPAQLRLTLMRGGDEQPLWTHLQQAKHQGQALNYSGTAYLCSPNYELTKSAQIYQHN
                     FEVIGKSGYSGNIPDANPREIVLDLLTNQRYGCGFPSQNIGDTDRYSNYCRAVGIFLS
                     PAYTEQGEAQRNISELLEQTNSAAVFSQGRLKIIPYGDGSHSGNGAVYVADNKAAYDL
                     TDDDFIVSGAQDPVKAGRKTNADAFNQIQVEYLDRDNDYNVAIAEAKDQANIEQYGLR
                     PKDAVRMHGICDAKVAQKVAQQLLQRALYVRNEYEFKLGWKYCLLEPMDIVTLTDAGL
                     GLNKTPVRITEIEEDGEGVLSVKAEDCPAGVYTVSEYPTQPSSGYSADYNVSPGNAHV
                     PVIFEAPLQLTGGEPQIWLATAGGGMWGGAEVWVSADGDSYTRVGAVNRKARFGALTA
                     DLPDGAVFDRTNTLGVEISAGQLTGGTEQDSRDLLTLCYVDGEFLAYADAELKGVGRY
                     TLGNLTRGAYGSAVNAHAAGSRFARIDEALFKYAVPRNWIGRTVWVKLVSYNVFGGGI
                     QDLAEVPAYSYTIEGAPLGQIQNLRLTSSWAYGKEAVIAWDKLDGADTYDVEIYAGGS
                     RRRLRAVDGIVDNSYTYTQADMKADGGQVRGIVFKVRGRAVTGKTGNWAQIAAQNPQL
                     QALQGISIDSGLRQAFFTCQKPAEEDFAGIIVWVCENAACPAADANKAYDGAETFITI
                     AKCGGKPLEKGKTYYLRAAGYDSFGKDNLKISGSISFTVYDVSATDLSESSLNKALRD
                     KINLIDGNGAGSVNERVEAVRSTADGNAAAVQTHARSINGLEAQYTVKVDANGKVAGF
                     GLATTPKNGTPESKFIVNADRFGIGAAGKADVFPFVVDTQKNRVGINGELVVNGKAVV
                     DKLNAGDIHGGKIAADTLDANRLKAGSVTAREIGAAAVTADKIGANAVTADKIQVADL
                     SAVSSNLGSITGGSLNIGGGNFTVSSDGILTADNAVIRGRIEADSGYFNGTVRASSVE
                     GDVLRAHRLRWTEGNVWVLDLDKDPLPRVLIPNFYVVSETFGNNRVQAKLLLNGGVLA
                     PREVRETENYTNYIWRGRTFGRYEDLPPRARGGGDYTEQSGSRYRTRLEYQIQVIPAG
                     KPVSLKLSLASHESVFSPFVSVSYLAQSDYEYKQLLGRMVWRTFAESFRYDSKKQIYL
                     GGDRRVHDYQNQMYKHWNAYGGLLQLPDDIYGISFEYRPFTNADWSTMLAFDRSDKFV
                     VVKKYRGHAPQQYSLFQEEFNTAVPKSNLLFFVEHWWQYIELRNIRVLIPESRENEVW
                     PSV"
     gene            6981..7259
                     /locus_tag="ACSIGJ_001080"
     CDS             6981..7259
                     /locus_tag="ACSIGJ_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHQ1329957.1"
                     /translation="MESQTFASVFDALCDTPAEAANMRLRAGLMMHIADTVRENGWTQ
                     KQAAEHCGLTRPRINDLLNGKIDKFSLDALVNINAGLGQCISLSFAPA"
     gene            complement(7287..7718)
                     /locus_tag="ACSIGJ_001081"
     CDS             complement(7287..7718)
                     /locus_tag="ACSIGJ_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HHQ1329958.1"
                     /translation="MPSETDLRARIVEEARSWLGTPYHHHAMVKGAGVDCAMLLVAVY
                     GAVGLLPEGFDPRPYPQDWHLHRDCERYLGFVTQFCRETESPQAGDIAVWRFGRSFSH
                     GGILAGGGKVIHSYIGRGVVSDDIGQAELIGRGVRFFTFSF"
     gene            complement(7720..8574)
                     /locus_tag="ACSIGJ_001082"
     CDS             complement(7720..8574)
                     /locus_tag="ACSIGJ_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HHQ1329959.1"
                     /translation="MKAATKELIDLLHGGDEFQMADLYTITLSGGRVLRHTGADMPVV
                     WDGQAYGAHELVIKRGATRTAVGLEVDSNTLQISAAPDYRLEGLQWAEAALGGVLDGA
                     RVRIDRVFFDAGLRPVGAVNIFSGRVSDVSGGRSSVKVDVKSDIELLNVSSPRNIYQA
                     GCMRTLYDDGCKVNREKFTVNGRVTENSRTGTVLKHNLTQPDGWFSQGVIKFAGGRNA
                     GLSRTVKAHGGNTFELALRLPYPPQAGDAFKVYPGCDKRRDTCKDKFDNIVHFRGFPF
                     IPSADTVV"
     gene            complement(8571..9170)
                     /locus_tag="ACSIGJ_001083"
     CDS             complement(8571..9170)
                     /locus_tag="ACSIGJ_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HHQ1329960.1"
                     /translation="MGNAVFPTFPGLKWGRKKTAVWSTGTQKSASGREFRTAYYTYPQ
                     WRFSLSFEVLRTKASVNELEKLAGFFNARKGSFESFLYEDPADNAVTDQPVGNTVQGV
                     ARYQLVRSMGGFIEPVSAVKERPAVKVGGTALAYGRDYTVTDKGVLVFNTPQPPGRPI
                     TWTGGFYFRVRFTSDTVDFENVLGSLWAAKKIEFTSVKL"
     gene            complement(9170..9436)
                     /locus_tag="ACSIGJ_001084"
     CDS             complement(9170..9436)
                     /locus_tag="ACSIGJ_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329961.1"
                     /translation="MIAHVCASTGWTWDYVADNLDLPRIKHLNEYWREHPPVHILAAS
                     YMGVKPSSGSVQSEADEAEAVGMLGGGELSEDEFDALLKAKGII"
     gene            complement(9448..9777)
                     /locus_tag="ACSIGJ_001085"
     CDS             complement(9448..9777)
                     /locus_tag="ACSIGJ_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329962.1"
                     /translation="MTVRIKGVTVELNGADYVIPPIALGALEQLQERIGSFDGNAADA
                     GQISTVIDCAHAALKRNYPDLTREEAADLIDIGNMNEVFAAVMDVSGLKRKEQEAAQA
                     GEARAAD"
     gene            complement(9838..10611)
                     /locus_tag="ACSIGJ_001086"
     CDS             complement(9838..10611)
                     /locus_tag="ACSIGJ_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329963.1"
                     /translation="MQLTFGSGEVFAQMITDAYGNRVQNATPVRIMGLQEMSVDLSAE
                     LKEFYGQNRYPLAVAQGKVKVSGKMKGALINGLTLNTLFFGTEYATGTMKALWAETTG
                     KVLDGDNYSYLQAAAPGGGKFAEDAGVMGQDGTAYIKVASSPKQGQYTVSESGVYAFN
                     SSDKGKTVYPSFTYTQTMPPAKKIELTNMATGNTPTFKMRYLTQFKGKKALLELESVT
                     SGKLGLFSTKNDDFSVPEIDFTASTDEAGFKIGTLWIQE"
     gene            complement(10637..11065)
                     /locus_tag="ACSIGJ_001087"
     CDS             complement(10637..11065)
                     /locus_tag="ACSIGJ_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329964.1"
                     /translation="MNREAVYSALWAKLDALDGFVTKSRKLVHWNDVKRYDQPALFMA
                     QGDMQALTLTGRETKWILRADVYLYVQTAGQPPAPVMNPLIDAVCNAVNAVHPVTGKT
                     DLTADGADIEYCRVEGTVETDEGTLGEQAVCIIPIVICAA"
     gene            complement(11062..11544)
                     /locus_tag="ACSIGJ_001088"
     CDS             complement(11062..11544)
                     /locus_tag="ACSIGJ_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HHQ1329965.1"
                     /translation="MLKMEFIGGGILAAVFKAYAADVQDAVVKSVGRSALRLQSEVVL
                     NRLSGQVLRVRTDNLRRSVHQRVNVSGNVVSGEVDTNVRYGIAHEYGFTGNVNVKASL
                     RQVKQAFGKPLKPPRYVRVRAHTRDVKLPERSFLRSALRDLTPKFADDLQKSIGKVLK
                     "
     gene            complement(11544..12074)
                     /locus_tag="ACSIGJ_001089"
     CDS             complement(11544..12074)
                     /locus_tag="ACSIGJ_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HHQ1329966.1"
                     /translation="MAAPVSLEEFKQRIGVEHDRRDDFFLSVIDGVSAAAEAYIGRSL
                     LAADYVGRYDGNGKDRIVLDNYPVLSVSSVKINGADAGGWEFDNWLLMRPEGFARGLK
                     NVEVSYRAGYERMPADIREAVMIIAVQRVNEIEGKGVRSKTLAGETVAFSTFGNSGGM
                     PPSAFAILNEYKRKGV"
     gene            complement(12077..12433)
                     /locus_tag="ACSIGJ_001090"
     CDS             complement(12077..12433)
                     /locus_tag="ACSIGJ_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329967.1"
                     /translation="MPSENQTAFSFGDFQMTVKLKAPEGFTDVSFGSQSYAADENGIV
                     EVPSEAAEFLYQFGFGNVASEPAEGPEKAKRGRKPKTGQPAGQSEPAEAAEPAEAEAA
                     EPAEAEAEPAEAEKAE"
     gene            complement(12440..13939)
                     /locus_tag="ACSIGJ_001091"
     CDS             complement(12440..13939)
                     /locus_tag="ACSIGJ_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329968.1"
                     /translation="MNVNQLTQETIELMKSAQAGGGPPDKGFTQPAGFTAGLQTYDLS
                     APSQKLYPVLTPLRNRIPRVGGGRTIGSNWKAVTNINVGNQRAGIGEGRRGGVINHET
                     VERNAQFRAIGLENQVTFEADYAARGFEDVKALAVAQTLQATMVAEEMILLGGNTSLK
                     AGVTPTPTAVVSADAAGKISVSTLSVICVALGLQAYWDVAGANNGATGQSPNIKTAQV
                     PAKITRRNADGTTDTFGGGSARKSAAASVSGIEAGKKVTAVIPAVRGAVAYAWYWGAA
                     GSEKLGAVTTAAKVEISADAEGTQTAASLPSEDNSTSILEFDGLLTQIALPDSGAFWS
                     DNKGGGLTSDNAGGVYEFEEAFAHFFTRYRLSPDTVYVNARDLAALTKLIIGNSGAPL
                     IKLNVDVNNTANIRAGVVVGSYMNKITGDDLNIVVHPNLPAGTYLFYSSRLPAYVQGV
                     GNLLQVRTRQEYYQIEWPLRTRMYEYGVYADEVLQGMFMPAFGMITNVG"
     gene            complement(13980..15137)
                     /locus_tag="ACSIGJ_001092"
     CDS             complement(13980..15137)
                     /locus_tag="ACSIGJ_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HHQ1329969.1"
                     /translation="MTKLYAQIAKTEAQDDGTVKVWGYASSEAVDSDGEVVAAEAMKA
                     AIPDYMKFGAVREMHGSNAAGTAIEINVEDDGRTFFGAHIVDPVAVTKVKTGVYKGFS
                     IGGRVTARDDLNKSQITGLKLTEISLVDRPANPDAVFTCFKADKPKDEAGAADKDGKP
                     SDKPTEEEDENPKDGDKGPKTEDKGDKDAGKKDEAGKSASVNLSESEIAALKAVLAKA
                     DKPKGGTAAKSMYQVKSPADVLMSLKWLVEDAAYDNIDEAVTAQIKESAAGLAESLKA
                     LAASEADKPADGLAAKAGKSGDLAKAESADELAKAQDALKKSNDALAKAQAEIESLKK
                     QAVPPKGSTKAISKAEDNGEDPLKGFQPIVKNDGTLDDVATLIKAKQTGRL"
     gene            complement(15205..17352)
                     /locus_tag="ACSIGJ_001093"
     CDS             complement(15205..17352)
                     /locus_tag="ACSIGJ_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HHQ1329970.1"
                     /translation="MSKKTPLSQGFIARVAAGVRYAFTGNADGWFDAGEPPAPAAQQA
                     EGRRFDYEPFYNVGHSKPREREAVGFAQLRALADNYDVLRLVIEARKDQMECLKWTIQ
                     KRDVESTEDDESQRKDRKVDEAVAFFRSPDKEHTWADWLRILLEDLFVIDAPCIYPRK
                     TLGGGLYALEVMDGATIKRVLDNTGRMPLPPDTAYQQILHGMAAVDYTADELIYRSRN
                     NRSYKVYGYSPVEQIIMTVNIALKRQVHALEYYTAGSVPDALVGVPETWSADDIRRFQ
                     EYWDLLLSGETAQRRKMRFVPGELSRNFRETKQPPLKDVYDEWLARVVCFAFSVEPTP
                     FVAQVNRSVAETSREQSLSDGMGSLKNWVKALIDDVLARYMDMAAYEFVWKGEESLNP
                     KEQAEIYAIYKNAGILTADEIRAELGKEPLPGQGQPEPDKQDGRKPEEPPNQGAEKLG
                     KSESPMSEDESAALIEAYLLTRIDGLAEQIAALIEGAAVDWQAGDLAAELSRAAGVVA
                     NGLDFGDWSGLSDVVEPIIRRVAEDGAVAALLRVMPEPAAGMVTNIRSRAVKWAHERA
                     AEMVGMKRAGGGLVRNPAAEWQITEGTREMIRAQVAEAMRNGDSVQELAGRLKESHAF
                     GNARARTIARTETAMADGMGNLIGWEGTGLVAGKQWITAKDDKVSDVCNANGGMGVIG
                     LHEPFSHGALTIPGHPNCRCAVVPVLAGDMPES"
     gene            complement(17349..18770)
                     /gene="terL"
                     /locus_tag="ACSIGJ_001094"
     CDS             complement(17349..18770)
                     /gene="terL"
                     /locus_tag="ACSIGJ_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HHQ1329971.1"
                     /translation="MALGQFDDVETSVIRSLSSASLYMFTRRMFYQRRGYVWQRANHH
                     APICNALERVFNGETKRLIINIPPRYSKTEIAVVNFIAWAMGRVPDCEFIHASYSAAL
                     AVNNSVQIRNLVQHEEYRAIFPDLALAGESGHHWKTTAGGVMYATGAGGTITGFGAGR
                     HREGFGGCIIIDDPHKADEARSEVRRQNVIDWFQNTVESRKNSPDTPIILIMQRLHEK
                     DLAGWLLDGGNGEEWEHLCLPAIQEDGTALWPEKHDIETLRRMEQAAPYVFAGQYLQK
                     PAPPDGGTFKPDNLQFVKALPAGNIRWVRAWDLASTANGGDYTAGGRLGVTEDGRYII
                     ANVVRGRYGADERDRILRNTAQKDGVKTKISIPQDPGQAGKSQTLYLTRQLAGFSVSA
                     GPESGDKVTRAGPFAAQVNIGNVMVLDDGTWDTDALIAEMRMFPNGRHDDQIDCLGRA
                     FGELLDTRTGMIDFLRSQVEAVK"
     gene            complement(18832..19281)
                     /locus_tag="ACSIGJ_001095"
     CDS             complement(18832..19281)
                     /locus_tag="ACSIGJ_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329972.1"
                     /translation="MSGTKRKLGRPTDYTKDMADKICEKIANGRSLRSICAEDGVPPM
                     KTIYRWLEANEEFRHQYARAREKQADYFAEEIIEIADSAQAESAAVSKAKLQIDARKW
                     AASKIAPKKYGDKTELDVKSGDGSMRAAVRLDAEEYRKIAEDVLRRV"
     gene            complement(19574..20443)
                     /locus_tag="ACSIGJ_001096"
     CDS             complement(19574..20443)
                     /locus_tag="ACSIGJ_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRO-N domain-containing protein"
                     /protein_id="HHQ1329973.1"
                     /translation="MNVIQSFNFNTTSPVRAFEKDGLTWFVAADVCKALEIQNPTQAL
                     EKLDDDERSMFNIGRSEIHGGGGNVNIINESGLYILILRSRKAMEQGSTAWKFRKWVT
                     SEVLPTIRKTGGYQVGQKTTADDRTGLRQAVAALVGRKGIDYSSAYSMIHQRFNVESI
                     EGIPAGKLPEAVAYVHALTLHTGLTGEVLDREPLPAPQPALPISGNALYDLAVAVSYG
                     ARAIQMGRDVSLPLKQLGCRQAVTMWTVWAETRSRLKAAANALEALSAHADAEHAEKI
                     RPILPEIRNLSAV"
     gene            complement(20725..21885)
                     /locus_tag="ACSIGJ_001097"
     CDS             complement(20725..21885)
                     /locus_tag="ACSIGJ_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HHQ1329974.1"
                     /translation="MNAAAVSAVFKERIASHAEHVKKVAHICTTEETTKQALILPLLD
                     ILGFSAFDPNKVRAEYQADFPGAKSGERVDYALFCNGAPVMFIEAKSYTENLSNHCPQ
                     LSRYFNATPEIAICAITNGREWRFFTDLSNKNIMDSEPFLTVDVTMLNENDMAQLYQF
                     RHDKFQPDALRSLAEESIYLTAFTESITESLKEVDLDFVRYVAGRANIQRQFTQRYLE
                     SIRHIVKQAVQNTVSSMVVSGLSAPKVQEEAAPVEKEQEDPTAPIIDPENNKIVTTYS
                     ERRLFDLVKSILPDDASIEAKDTESYFGVLVDGKSNRWILRYFDNKQRPSVIFPIELE
                     ESDISNIERCGLEVSGNQVIIDTPENLLRVVWLVIDSYRFCCDDENFKRKPK"
     gene            complement(21976..22383)
                     /locus_tag="ACSIGJ_001098"
     CDS             complement(21976..22383)
                     /locus_tag="ACSIGJ_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329975.1"
                     /translation="MNQQEFEFMNDLARAFERRYRDTRSLNRCLSIEGRYMGEEACPH
                     KPEIGLRYGEDAMFLTLQAWAKVDAPQQEAVRISFGIGAKSQAAYEERLQAEIRRRGE
                     GPLHLQTDLGLAAWYRAIRQAAGNDFDLLFEKV"
BASE COUNT         4779 a         6962 c         5648 g         5108 t
ORIGIN      
        1 tctgtttctt tccaccacgc tttctgctcc tgattaaaat ttcccatttc ttgcagcatc
       61 gctaaaagca cagggtttga cttgccattc actttttcgt gagcgccaag gtatttacgg
      121 gcttcttcaa tccatttcaa ttctttcatt tcttttcctt ccgataaaag acgagatgta
      181 aactttcggt ttacaccccg gccaagcctt tgattgcagg tttccgcaac accgatacat
      241 ccggtttcac acgttccgtt tccatctcca ctcctgttac acgccgccgc ttggcaaaat
      301 ccgtttcagg cggcataatg tatcgacaac gtatctacaa cacgaaagca aaaccatgtt
      361 gcgactgcaa aaatggggca acagcgcggc agtccgtctg cccgcccgcc tcctgaccgc
      421 cctcaacgcc aaaatcggcg acgcgctgga aacagaaatc cgcaacggcg agctgctcat
      481 ccgccccgtc aaacgctacc gcctgtccga ccttttggcg gaaacggaag ccgccccgcc
      541 gcgcgcggaa ggctgggaag aaatgcccga tgtcggacag gagggcgttt gatgtatatt
      601 cccgagcgag gcgacatttt ccatcttgcc ttcgaccccg ccgccggtac ggaaatgaaa
      661 ggcggccatt acgccatagc cctgtcgccc agagcttaca accgcgcaac cggcttggtt
      721 tacgcctgcc cgatttcaca aggcagggcg gcggcggcaa gaagcggcgg tatgatttcc
      781 acactgctcg gcgcgggaac ggcaacacaa ggcaatgtcc actgccaccg gatgaaggca
      841 ttggattgga aaatccgccg cgccgccttc cgcgaaaccg tccccgatta cgtgattgaa
      901 gacgtattgg cacgcatcgg cgcagtactg ttcgactgac ttcagagtgc gaagcctgtc
      961 cgcaggggga agcatccgtt tccatacccg cccctatttc aaagcattca aaatatccgc
     1021 cggcttccaa atcaacgccg tcaaaggcgc ggcgcccaaa accgcataaa acatacggcg
     1081 caacaccgca tactcggcaa acagcttcaa catcttgcga aactccctgt ttatgctaaa
     1141 cttcaaacag atttctcctt acctcggata aggttaaatt cagaaacgcc gcagggattc
     1201 cgcccctcgc ggcgtttcgc cgttcacacc ccgcgcgtaa tgtcgtgcgt cagccggacg
     1261 cgcccggaac acagcgtctt cacgcgccgc gataccgctc gaaatcaatc ttttccatcc
     1321 gaagaccctc cgtctttcac acccgtaagc ttttccgcca agccgcccag ataaccgcgc
     1381 aaaaccttgg gcgcgagcgc gcgcaccgta tcgagcgcgt gccccgtcat catccccgcg
     1441 aacacccccg ccgccgcgca cgtccacgcc tgatccagcg gcacgaaccg ctccgccgcg
     1501 gccgccgccg cgattgcaga aataaaagcc tccagcacag cctgcacggg gcggccgcgt
     1561 tcctgaaggc ttgcccacac cgcgcccagc acgccgccgg ccgccgcaaa cagcaaaccg
     1621 aactgcaaga accccattca cgacccctca tccgccaaac cccgcgactt ttgcgccgaa
     1681 aacaaaaacc tcaacgcatt attccccgcc agcgcgcaga aaaacgccaa cagcggcgga
     1741 acgaccatac ccgtgttcaa aggcgggtac gccccccaaa acgccaccga aaccaaaaac
     1801 cacacaaagc ccgaaaacag caaaaggtag gccgaagcca cacaagccct cacgccccgc
     1861 gcaaacagca atgccgtctg aagcagcccc gccgccgcca gcacccccac cgtccacgcc
     1921 tccggaatat cctggaattt gtaataaatc cgccacgcgt aaatctcgtc gggcgcaagc
     1981 gcgaacaccg ccgcgtaacc cgacaagccc aaaccgctga ccgcctccag cacccgcgtc
     2041 gccgtgccga acaaccacgc ctgaaacccg gcgggcaaaa accgccgttc cgtcaaaaac
     2101 ctcaacatac ccgtcacaca ccccccggca aaacaccgcg aagccgaagc cccgcagtgt
     2161 ccgacgccgt ctgaaaactt attccgcgta aaccggttgc gcgtctttgg ggatgcccgc
     2221 cgcaccttcc agcgccttgc ggtacaccca accggggtcg ggcgcggtta tgtccgtttc
     2281 ctgaaaatcc aatgtgcgcg ccaacaggta gcgtttgccc gatttaaacg cattttccga
     2341 cacatagccg ttcaacgtcg ccgtaacata accgttgcgg taatccgcgc cgatatgttc
     2401 gacgacgtgg taggccgcac tcgcgccggt cataccgtcc tcgactgcaa aattcaaggc
     2461 aacaatcttt ttatcttcaa tcatttcaaa tctccaaaag cccgctaggg gcgggaacga
     2521 aaatgccgtc cgaggtcttc agagggcatt gcccggacaa aacaggaaaa accgcagagc
     2581 cggcatacgg gggcgtcaga cggagggcca aacctcgttt tcgcgcgatt ccggaatcag
     2641 caccctgata ttgcgcagct cgatatactg ccaccagtgt tccacaaaaa acagcaggtt
     2701 ggatttcgga acggcagtat taaactcctc ctgaaacaga gaatactgtt ggggcgcatg
     2761 accccggtat ttcttaacca ctacaaactt atccgacctg tcgaacgcaa gcatcgtact
     2821 ccagtcggca ttggtaaaag gccggtactc gaacgatatg ccgtagatat cgtccggcaa
     2881 ctgaagcagc ccgccgtaag cattccaatg tttatacatc tgattttggt aatcgtgtac
     2941 acgccggtcg ccgcccaaat aaatctgctt cttactgtcg taccggaaac tttccgcaaa
     3001 ggtgcgccaa accatcctcc cgagcagctg cttgtattcg tagtcggatt gcgccaaata
     3061 cgaaaccgac acgaacggcg aaaacaccga ttcgtgggaa gcgagcgaca acttcaggct
     3121 gacgggtttg cccgcgggga tgacttggat ctggtactcg aggcgggttc tgtaccttga
     3181 gccggattgt tcggtataat ccccgccccc ccttgcccgg gggggcaggt cttcgtacct
     3241 cccgaaagtt cggccgcgcc aaatataatt ggtatagttc tcggtttccc tgacttccct
     3301 cggcgcaagc acacccccgt tcaacagcag ctttgcctgc accctgttgt tgccgaacgt
     3361 ttccgaaacc acataaaaat tcggaatcaa aaccctcggc agcgggtctt tatccaaatc
     3421 caacacccaa acgttaccct ccgtccaacg cagcctgtgc gccctcaaca cgtcgccctc
     3481 gacggacgaa gccctgaccg taccgttgaa ataacccgaa tcggcctcaa tccggccgcg
     3541 tattaccgca ttgtcggccg tcaggatgcc gtctgaagac accgtaaaat tacccccgcc
     3601 gatattcagg ctgccgcccg tgatgcttcc gagattggaa gataccgcgc tcaaatcggc
     3661 aacctgaatc ttatcggcag tcaccgcgtt tgccccgatt ttatcggcgg taacggcggc
     3721 agccccgatt tcccgcgccg taacgcttcc ggctttcagg cggtttgcgt ccagcgtgtc
     3781 cgccgcgatt ttgccgccgt ggatatcccc ggcgttcaac ttatcgacaa ccgccttgcc
     3841 gttgaccacc aattcgccgt tgatgcccac gcggtttttc tgcgtgtcca ccacgaacgg
     3901 gaacacatcc gccttcccgg ccgcaccgat gccgaagcgg tcggcattca cgataaactt
     3961 gctttcgggc gtgccgtttt tcggcgtagt cgccaagccg aagcccgcca ctttgccgtt
     4021 tgcatcaacc ttgaccgtgt attgcgcctc caagccgttg atgcttcggg cgtgtgtctg
     4081 aaccgccgcc gcattgccgt ccgccgtcga tcggactgcc tcgacgcgct cgttgacgct
     4141 gcccgcgccg ttgccgtcaa tcaggttgat tttgtcgcgc aaagccttgt tcagactgct
     4201 ttccgacagg tcggttgccg atacgtcgta aacggtaaac gatatgctgc cgctgatttt
     4261 caggttatct ttgccgaaac tgtcgtaacc cgccgccctc aagtaatagg tctttccttt
     4321 ctccagcggt ttgccgccgc atttggcgat ggttataaac gtttccgcgc cgtcataggc
     4381 tttgtttgcg tccgcggccg ggcaggctgc gttttcgcaa acccagacga taatccctgc
     4441 aaagtcttct tcggcaggtt tttggcaggt aaaaaacgcc tggcgcaggc cgctgtcgat
     4501 agagatgccc tgcaatgcct gcaattgcgg attttgcgcc gcgatttgcg cccaattgcc
     4561 cgttttaccg gtaacggcac gcccgcgaac cttgaagaca ataccgcgta cctggccgcc
     4621 gtccgccttc atatccgcct gagtgtaggt gtagctgttg tcaacgatgc cgtcaaccgc
     4681 acgcagacgg cgtcggctgc cgcctgcgta gatttccacg tcgtaggtat ccgcgccgtc
     4741 caatttatcc caagcgatga cggcttcttt gccgtatgcc cacgatgatg tcaggcgcag
     4801 gttttggatt tgaccgagcg gcgcgccttc gatggtgtag gaatacgccg gcacttccgc
     4861 caaatcctga atgccgccgc cgaaaacgtt gtacgaaacc agtttgaccc aaaccgtgcg
     4921 gccaatccag ttgcgcggca cggcgtattt gaacaatgct tcgtcgatgc gcgcaaaccg
     4981 gctgcccgcc gcgtgtgcgt tgacggcgga gccgtacgcg ccgcgcgtca ggttgcccaa
     5041 tgtgtagcgt cccacgccct tcagttcggc gtcggcgtat gccagaaatt cgccgtcgac
     5101 gtagcacaat gtcagcaaat cgcggctgtc ctgctccgtg ccgcccgtca gctgccccgc
     5161 cgaaatttcc acgcccaacg tatttgtgcg gtcgaaaacc gcgccgtcgg gcaaatcggc
     5221 ggtcagcgcg ccgaaacgcg ccttgcggtt gaccgcgccg acgcgggtgt agctgtcgcc
     5281 gtccgccgat acccacactt cggcgccgcc ccacataccg cccccggcgg ttgccagcca
     5341 gatttgcggt tcgccgcccg tcagttgcaa cggcgcttcg aaaattaccg gcacatgggc
     5401 gttgcccggc gaaacgttgt agtcggccga ataacccgaa gacggctgcg tcggatattc
     5461 cgacacggta taaacaccgg ccgggcagtc ttcggccttg acggataaaa ccccttctcc
     5521 gtcttcttcg atttccgtaa tgcggacggg cgttttattc aggccgagcc ccgcgtcggt
     5581 cagggttacg atgtccatcg gctcaagcag gcagtatttc caacccagct taaactcata
     5641 ttcgttgcgc acgtacaggg cgcgttgcag cagttgttgc gccacttttt gcgccacctt
     5701 cgcatcgcaa atgccgtgca tcctgaccgc gtctttcggg cgcaatccgt actgctcgat
     5761 attcgcctgg tctttcgctt cggcgatggc gacgttgtag tcgttgtccc tgtcaaggta
     5821 ctcgacctga atctgattaa acgcatcggc attggttttg cgcccggcct ttacagggtc
     5881 ttgcgcgccc gaaacgatga aatcgtcatc ggtcaggtcg taggcggcct tattgtcggc
     5941 aacgtacacc gcgccgttcc ccgaatggct gccgtccccg taggggatga ttttcaggcg
     6001 gccttgcgaa aataccgccg cgctgttggt ctgctccagc agttcggaaa tattccgttg
     6061 cgcctccccc tgttccgtgt aggcagggct taggaaaatc ccgacggcgc ggcaataatt
     6121 gctgtaccgg tcggtatcgc cgatgttttg ggacgggaaa ccgcagccgt agcgttggtt
     6181 cgtcagcaaa tccaatacga tttcgcgcgg gtttgcatcg gggatgttgc cggaatagcc
     6241 cgatttcccg atgacctcga aattgtgctg atatatttgc gccgatttcg tcagttcgta
     6301 gttcgggctg cacaaataag ccgtgcccga ataattcaag gcttggcctt ggtgcttcgc
     6361 ctgttgcagg tgcgtccaca acggctgctc gtcgccgccg cgcataagcg tcaggcgcaa
     6421 ctgtgccggc gaatcgaatt tctccttgtc gcgccaaatc cgccccacgc cctgaatctc
     6481 gccctcgcac aaagcaagca tgacggcggc ttcgtaggta taggaaatac cgacctgttt
     6541 cacaccgccg ccgcccttgc cgccccggcg cgtcgtagtt ttatgctcga tggtgacgaa
     6601 atcgccgtac caaatcaaat tgcccgccac gcgcgtcctg ccgtagatga cgggcagggt
     6661 cagcccctgt gatgaccgct gaacctgtaa cgataaaatc cgctcttcgg cagatgtaac
     6721 ggttgacgat ttaccaccca taaaaccacc tcgaaacttg caaaaattat caaaatcgat
     6781 ataatttact tatatcaaaa cagatataaa atatgaaacc gttaaatttt tcaggcggtt
     6841 cattggattg cctgcggcaa ttccccgaaa acgccaaaca ggcggcaggt tatcaacttc
     6901 aagaaaagcc ggaaaaccgc gggcgccgat tcggaatcgg cggaaaaacg ttacaacaaa
     6961 ttgattcggg aaaagaaata atggaaagcc aaactttcgc ctccgtattt gacgcactgt
     7021 gcgacacgcc tgccgaagcc gccaatatgc ggttgcgcgc cggcctgatg atgcacatcg
     7081 ccgataccgt ccgcgaaaac ggttggacgc aaaaacaggc cgcagaacat tgcggcctga
     7141 cccggccgcg catcaacgac ctgctgaacg ggaaaatcga caaattttca ttggatgcgc
     7201 tcgtaaacat caatgccgga ctcgggcagt gcatttcctt atccttcgcc cccgcgtaaa
     7261 gcccgtcctt tcagacggcc gcaaaatcaa aatgaaaatg taaaaaaccg aaccccgcgc
     7321 ccgataagtt cggcttggcc gatgtcgtcc gacaccacgc cgcgcccgat gtagctgtga
     7381 ataaccttgc cgccgcccgc caatatgccg ccgtgcgaaa acgaccgccc gaaacgccat
     7441 actgcaatgt cgcccgcctg cggcgattcc gtttcacggc aaaattgcgt gacgaacccc
     7501 aaataacgct cgcaatcacg gtgcagatgc caatcttgag ggtaagggcg cgggtcgaac
     7561 ccttcgggaa gcagtccgac cgccccgtag acggcgacca ggagcatcgc gcaatcaaca
     7621 cccgcgccct tgaccattgc atgatgatgg tacggcgtgc caagccacga ccgcgcctct
     7681 tcgacgatcc gcgccctcaa atccgtttcg gacggcatat cacaccaccg tatctgccga
     7741 agggatgaac gggaagccgc gaaaatgcac gatattgtca aacttatcct tgcaggtatc
     7801 gcgccgcttg tcgcagcccg gataaacctt gaacgcatcg cccgcctgcg gcgggtaggg
     7861 caggcgaagg gcaagttcga acgtgttgcc gccgtgtgcc ttgaccgtcc tgctcaaacc
     7921 cgcgttgcgc ccgcccgcaa acttaatcac gccctgcgaa aaccagccgt caggctgcgt
     7981 cagattgtgc ttcagcacgg ttcccgtccg gctgttttcg gttacgcgcc cgtttaccgt
     8041 gaatttctca cggttgacct tgcagccgtc gtcatagagc gtcctcatgc agcccgcctg
     8101 ataaatgttg cgcgggctgg aaacgttcaa aagctcgatg tcggatttca cgtcaacctt
     8161 cacggacgac ctgccgcccg atacgtccga cacgcgccct gaaaaaatat tcaccgcacc
     8221 gacggggcgc agtccggcat caaaaaacac acggtctatc ctgacccgcg cgccgtccaa
     8281 tacgccgccc agggcggcct ccgcccattg caggccctca agcctgtaat cgggcgcggc
     8341 tgaaatctgc agggtgttgg aatccacttc caatccgacg gcggtacggg ttgcgccgcg
     8401 cttgataacc agctcgtgcg ccccgtaggc ctgaccgtcc caaacgacgg gcatatccgc
     8461 gccggtatgc cgcagcaccc ggccgcccga aagcgtaatg gtgtacaaat ccgccatctg
     8521 aaactcgtcg ccgccgtgca gcaaatcaat cagttctttt gtcgccgcct tcatagtttc
     8581 acactcgtaa actcaatctt tttggccgcc cacaagctgc ccaaaacgtt ttcaaaatcc
     8641 accgtgtcag acgtaaacct cacgcggaaa taaaagccgc ccgtccatgt aatcgggcgg
     8701 cccggcggtt gcggcgtgtt gaaaaccaaa acgcccttgt cggtaacggt gtaatcgcgc
     8761 ccgtacgcca acgccgtgcc gccgaccttg acggcgggcc gttccttgac cgccgacaca
     8821 ggctcgataa atccgcccat cgaacggaca agctgataac gcgcaacgcc ctgcaccgtg
     8881 tttccgacag gctggtcggt tacggcgttg tcggccgggt cttcgtaaag gaaactttca
     8941 aagctgcctt tgcgcgcgtt gaagaatccc gccagttttt ccaactcgtt tacggacgcc
     9001 tttgtccgca atacctcgaa cgacagcgaa aaccgccatt gcgggtaggt gtagtaggcg
     9061 gttcggaact cacggccgct tgccgatttc tgcgtccccg tactccatac cgccgttttc
     9121 ttccgccccc acttcaagcc ggggaacgtg gggaaaaccg cattgcccat cagatgattc
     9181 ctttcgcttt gagcagggcg tcaaattcgt cttcggacag ttcgccgccg ccaagcatac
     9241 cgacggcttc ggcttcgtcc gcttcgcttt gcacactgcc cgacgacggc ttgacgccca
     9301 tatacgacgc tgccaagata tgcacgggcg gatgttcgcg ccaatactcg ttcaggtgtt
     9361 tgatacgcgg caaatccaag ttgtcggcga cgtagtccca cgtccagccg gtagaggcgc
     9421 aaacgtgggc aatcaccgcg ccgaaactta atccgccgcc cgggcctccc ccgcttgtgc
     9481 ggcttcctgc tccttgcgtt tcaagcccga aacgtccatc acggcggcaa acacttcgtt
     9541 catgttgccg atatcgatca aatcggccgc ttcttcgcgc gttaaatccg ggtaattgcg
     9601 cttcagggcg gcgtgggcgc aatcgataac ggtagagatt tgtccggcat ccgccgcatt
     9661 gccgtcaaat gagccgatgc gctcctgcaa ctgctccaac gcgcccagcg caataggcgg
     9721 aatcacataa tccgcgccgt tcagttctac ggttacgcct ttaatccgta cggtcatttt
     9781 tatttccttg actttggttg aataaaaaaa gccgcctttt cagacggcat ataaacatta
     9841 ctcctgaatc cacagcgtac cgattttaaa gcccgcctcg tcggttgagg cggtaaagtc
     9901 gatttcgggg acggagaagt cgtcgttttt agtcgagaac aagcccaatt tgccgctggt
     9961 tacgctttcc agttccaaca gggctttttt gcctttgaac tgcgtcaggt acctcatctt
    10021 aaacgtcggc gtgttgcccg tcgccatatt ggtcagctcg attttcttcg ccgggggcat
    10081 cgtttgcgta taggtaaagc tcggataaac ggttttgcct ttatccgagc tgttaaaggc
    10141 gtaaacgccc gattccgaaa ccgtatattg gccttgtttc ggggaggatg ctactttgat
    10201 ataagccgtg ccgtcctggc ccatcacgcc cgcgtcctca gcgaacttgc cgccgccggg
    10261 cgcggctgcc tgaaggtaag aataattgtc gccatcgagg actttgcccg tagtttccgc
    10321 ccaaagcgcc ttcatcgtgc cggttgcata ttccgtaccg aaaaacaggg tattgagggt
    10381 caggccgtta atcaacgcgc ctttcatttt gcccgacacc ttgaccttgc cttgcgccac
    10441 agccaaagga taacggtttt gaccgtagaa ctctttcaat tccgccgaca aatcgacgga
    10501 catttcctgc aagcccataa tccgcacggg cgttgcgttc tgtacacggt tgccgtaagc
    10561 atccgtaatc atttgggcga acacttcgcc gctgccaaac gtcaactgca tgacatccct
    10621 ttcaaaaaaa gccgcattac gcggcgcaaa tcacaatcgg aataatacaa accgcctgtt
    10681 cgccaagcgt cccctcgtcg gtttccaccg taccctcgac gcggcaatac tcgatatccg
    10741 cgccgtccgc cgttaaatcc gtcttgcccg taacggggtg gacggcgttc acggcattgc
    10801 acaccgcgtc aatcagcgga ttcatgacgg gcgcgggcgg ttggcccgcc gtctggacgt
    10861 acaggtaaac atcggcgcgc aaaatccact tggtctcccg ccccgtcaat gtcaacgcct
    10921 gcatatcgcc ctgcgccata aataacgcgg gctgatcgta gcgtttcaca tcgttccagt
    10981 gcaccagctt ccggctcttg gttacgaagc cgtccaatgc gtccagcttc gcccacagcg
    11041 cggaataaac cgcctcacga ttcatttcaa cacctttcca atcgattttt gcaggtcgtc
    11101 cgcgaacttc ggcgtcaaat cgcgcaatgc cgaacggagg aacgaccgct ccggcaactt
    11161 cacatcacgg gtatgggcgc ggacgcggac atatcgcggc ggtttcagcg gcttcccgaa
    11221 cgcctgtttg acctggcgca gcgaagcctt cacattgaca ttacccgtaa aaccatactc
    11281 atgcgcgatg ccgtaacgga cgttcgtatc gacttcgccc gaaacgacat tgccggaaac
    11341 gttcacgcgc tggtgtacgg agcgtctcag gttgtccgtc cttaccctca acacctgacc
    11401 cgataaccgg ttcagcacaa cttcgctttg cagtcgcaat gccgacctgc ccaccgattt
    11461 gacgaccgca tcttgaacat cggcggcata tgctttaaaa accgccgcca gaataccgcc
    11521 gccgataaat tccatcttca gcatcacacg ccctttcgtt tgtactcatt gagtatcgca
    11581 aacgccgacg gcggcatacc gccggaatta ccgaacgtag aaaaagcgac ggtttcgcct
    11641 gcaagcgtct tgctccgtac gcccttgccc tcgatttcgt tcacgcgctg caccgcgata
    11701 atcatcaccg cttcgcgtat gtcggcgggc atgcgctcat aaccggcgcg gtacgatacc
    11761 tcgacatttt tcagcccgcg cgcgaaacct tcggggcgca tcagcagcca gttgtcaaac
    11821 tcccagccgc cggcatccgc gccgttgatt ttgacggacg acaccgacag gacgggataa
    11881 ttgtccaaca cgatgcggtc tttgccgttg ccgtcgtacc gcccgacata atcggcggcc
    11941 aggaggctgc gcccgatata ggcttccgcc gccgccgaca cgccgtcaat gacgctgagg
    12001 aagaaatcgt cccgccggtc atgttcaacg ccgatacgct gcttgaactc ctcaagcgat
    12061 accggggcgg ccatcgttat tcagcctttt cggcttcggc aggttcggct tctgcttcgg
    12121 caggttcggc agcttcggct tcggcaggtt cggcagcttc ggcaggttcg gattgtcctg
    12181 ccggctgccc ggttttaggt ttacgcccgc gcttggcttt ttcaggccct tcggcaggct
    12241 cggaggcaac gttgccaaaa ccgaactgat acaggaattc cgccgcctcc gacggcactt
    12301 ccacgatacc gttttcatct gccgcgtagc tttggctgcc gaaggaaacg tcggtaaacc
    12361 cttcgggcgc ttttaatttg actgtcatct gaaaatctcc aaaagaaaaa gccgtctgat
    12421 tttcagacgg catatcggct taacccacgt tggtaatcat accgaaggca ggcatgaaca
    12481 taccttgcag cacctcgtcc gcatagacgc cgtattcgta catacgggtg cgcagcggcc
    12541 attcgatttg gtaatactct tggcgcgtac gcacttgcag cagattgccg acgccctgaa
    12601 cgtaggcggg caggcggctt gagtagaaca ggtaagtacc ggcaggcagg ttcgggtgta
    12661 ccacgatgtt caggtcgtcg cccgtgatct tgttcatata cgaaccgaca acgacaccgg
    12721 cgcggatgtt cgccgtattg ttcacgtcaa cgttcaactt aatcaacggc gcgccgctgt
    12781 ttccgataat cagcttggtc agcgccgcca aatcgcgggc gttgacgtag acggtatcgg
    12841 gggacaggcg gtatcgggta aagaaatgcg cgaacgcctc ttcaaattca tacacgccgc
    12901 ccgcgttgtc ggaagtcagg ccgccgcctt tgttgtccga ccagaacgcg cccgaatcag
    12961 gcagggcgat ttgggtcaac aggccgtcaa attccaaaat agaagtggaa ttgtcttcgg
    13021 acggcaggga agcggcggtc tgggtacctt cggcatcagc cgaaatttcc actttggcgg
    13081 cagtggtaac cgcgcccagt ttttcagaac cggccgcacc ccagtaccaa gcataggcaa
    13141 ccgcgccgcg aacagccgga atcacggcgg ttactttttt gcctgcctca ataccggaaa
    13201 cggaagccgc cgcagatttt cgggcggaac cgccgccgaa cgtatcggtt gtaccatccg
    13261 cgtttcggcg tgtgattttg gcagggactt gggcagtttt aatgttcggg ctttgtccgg
    13321 ttgcaccgtt gttcgcgcct gccacatccc agtatgcctg caagcccaaa gccacacaga
    13381 ttacagacaa ggtgctgacg ctgattttcc ccgccgcgtc ggcggaaacg acggcggtcg
    13441 gtgtaggtgt aacgcctgcc ttcaggctgg tgttgccgcc caacaaaatc atttcttcgg
    13501 caaccatagt agcctgaagg gtttgggcga ccgccaacgc tttcacgtcc tcgaaaccgc
    13561 gcgcggcgta atccgcttca aaggttactt ggttttccaa gccgatggcg cggaattgcg
    13621 cgttgcgttc caccgtttcg tgattgatga cgccgccgcg cctgccttcg ccgataccgg
    13681 cgcgttggtt gccgacgttg atattcgtga cggctttcca gttcgagccg atggtgcggc
    13741 cgccgcccac gcgcgggata cggttgcgca acggggtcaa taccggatag agtttttgag
    13801 acggcgcgga aaggtcatag gtttgcaggc cggcggtaaa gccggccggc tgggtaaaac
    13861 ctttgtcagg cggcccgccg cccgcttgtg ccgacttcat cagttcaatc gtttcttgtg
    13921 tgagttggtt cacgttcatt tatcgctcct gataataaaa aaaccgcctg taagcggtgt
    13981 tacagacggc ctgtttgttt tgccttaatg agtgtcgcca cgtcatccag cgtgccgtca
    14041 ttctttacaa tcggctgaaa accttttaac gggtcttcgc cgttatcttc tgccttactg
    14101 atagctttcg tactgccttt cggcggtact gcctgtttct tcaggctttc gatttccgcc
    14161 tgcgctttgg caagggcgtc attcgatttt ttcagcgcgt cttgtgcctt tgccagttcg
    14221 tcagccgatt ccgctttggc aagatcgcct gatttgccgg ctttggccgc caaaccgtcg
    14281 gccggcttat cggcttcgct tgccgccaac gctttcagcg attcggcaag gccggccgcc
    14341 gattctttga tttgcgcggt aacggcttca tcgatgttgt cgtaggcggc gtcctcaacc
    14401 agccatttca gcgacatcaa tacatcagcc ggtgatttga cttggtacat tgatttggcg
    14461 gccgtcccgc ctttcggctt gtcggcttta gccaataccg ctttcaaggc ggcgatttca
    14521 gattcggaca aattcacgct tgccgatttt ccggcttcgt cctttttgcc ggcgtcctta
    14581 tcgcctttat cttcggtttt aggcccttta tcgccgtctt taggattttc atcttcctct
    14641 tcggttggtt tgtcagacgg cttgccgtct ttatccgccg cccctgcctc atctttcggt
    14701 ttgtccgcct taaagcaggt aaacaccgcg tcaggattgg cagggcggtc gacaaggctg
    14761 atttccgtca gcttcaagcc cgtgatttgc gacttgttca aatcatcgcg ggcggtaacc
    14821 ctgccgccga tggaaaagcc tttgtaaacg cctgtcttga ctttcgtcac ggcaacaggg
    14881 tcaacgatat gcgccccgaa aaatgtgcgc ccgtcgtctt ccacgttgat ttcaatcgcc
    14941 gttcccgccg cgtttgagcc gtgcatctcg cgcaccgcgc cgaacttcat ataatcggga
    15001 atcgccgcct tcattgcttc cgccgcgacg acttcgccgt ccgaatcgac cgcttcgctc
    15061 gaggcatacc cccaaacttt gaccgtgccg tcgtcctgcg cctccgtctt ggcgatttgc
    15121 gcgtataact tcgtcattgg tttgctccaa aaaaaagccg ccccgcgaag gggcggcaaa
    15181 acacacccgc ctgacccgaa ggaatcaaga ttcaggcata tcccctgcca aaacaggcac
    15241 aaccgcgcac cggcagttcg gatgaccggg tatcgtcaac gcaccgtggg agaaaggctc
    15301 gtgcaaaccg attacgccca tcccgccgtt ggcattgcag acatcggaca ccttgtcgtc
    15361 ttttgcggtt atccactgct tgccggcaac cagccccgtt ccttcccagc ctatcaggtt
    15421 gcccataccg tccgccatcg ccgtttccgt tcgggcaatg gttcgggcgc gtgcgttgcc
    15481 gaaagcatgg gattctttca ggcggcctgc caattcctgc acactgtcgc cgtttcgcat
    15541 ggcttcggct acttgggcgc gtatcatttc gcgcgtccct tcggtgattt gccactcggc
    15601 ggcaggattt cggacaagcc cgccgcccgc ccgcttcatg ccgaccattt cggcggcgcg
    15661 ttcatgcgcc cacttgacgg cgcggctgcg aatgttcgta accataccgg cggcaggttc
    15721 aggcattacg cgcaacaagg cggcaaccgc cccgtcttcc gcaacacgcc tgattatcgg
    15781 ctcgaccaca tcggacaatc ccgaccaatc gccaaaatcc aagccgtttg caacaacccc
    15841 tgccgcgcgg ctcaattccg ccgccaaatc cccggcctgc caatcgacgg ccgcaccctc
    15901 aatcagcgcc gcgatttgtt cggccaagcc gtcaatgcgt gtcagcaaat aagcctcaat
    15961 aagcgcggca gattcgtctt cgctcatcgg gctttccgac tttcccaact tttcagcccc
    16021 ttggttcggc ggctcttcgg gctttcggcc gtcttgctta tccggttcag gctgcccctg
    16081 ccccggtaac ggctccttgc ccagttcggc gcggatttcg tcggcggtca agatgcctgc
    16141 gtttttgtag atggcgtaga tttcagcctg ttctttcggg ttgagcgatt cctccccctt
    16201 ccagacaaac tcatacgccg ccatatccat gtaacgggca agcacgtcgt caatcagggc
    16261 ttttacccag tttttcaggc tgcccatgcc gtctgaaagc gactgctcgc ggctcgtctc
    16321 tgccacgctg cggtttacct gcgccacgaa cggcgtaggc tcgacactaa acgcaaagca
    16381 gacgacacgc gccagccatt cgtcgtaaac gtccttcaac ggcggctgct tcgtctcgcg
    16441 gaagtttcgg gacaactcgc ccggcacgaa acgcattttg cgccgctgcg ccgtttcgcc
    16501 cgacagcagc aaatcccagt attcttgaaa ccgcctgatg tcgtccgccg accacgtttc
    16561 aggcacgccg actaaagcat cgggcacgct gcccgccgtg tagtattcca gcgcgtgaac
    16621 ctgccgtttt aaggcgatat tcacggtcat gatgatttgc tcgacgggcg aatagccgta
    16681 aaccttgtaa cttcggttat tccgcgaacg gtaaatcaac tcgtcagccg tgtaatcgac
    16741 cgccgccatg ccgtgcagga tttgctgata cgccgtatcg ggcggtaacg gcatacgccc
    16801 cgtattgtcc aaaacgcgct taatcgtcgc cccgtccatc acttcgaggg cgtacaagcc
    16861 gccgcccagt gttttgcgcg ggtagatgca cggcgcgtca ataacaaaca ggtcttccag
    16921 caagatgcgc agccagtccg cccacgtatg ttctttatcg ggcgaccgga agaacgcaac
    16981 ggcttcatcg acctttcggt ctttccgttg cgattcgtcg tcttcggttg attcgacgtc
    17041 gcgcttttgg attgtccact taaggcactc catttggtct ttacgcgcct cgataaccaa
    17101 acgcaataca tcgtagttgt cggcaagggc gcgtaattgc gcaaagccta ccgcttcacg
    17161 ttcgcgcggc ttggaatgcc cgacgttgta gaacggctcg taatcaaacc gccgcccctc
    17221 tgcctgctgc gcggcagggg ccggaggctc gcccgcgtcg aaccacccgt ccgcgttgcc
    17281 ggtaaaggcg taacggacgc cggcggcaac gcgggcaata aagccttgcg ataaaggtgt
    17341 ctttttactc atttcacagc ctcgacctgc gatcgcagga aatcaatcat gcccgtccgg
    17401 gtatccagca gctcgccaaa cgcacggccc aaacagtcta tttggtcgtc atgccggccg
    17461 ttcgggaaca tgcgcatttc cgcaatcagc gcgtccgtgt cccatgtgcc gtcgtccagc
    17521 accatcacat tgccgatgtt gacctgtgcc gcgaacggtc cggcgcgtgt aaccttgtcg
    17581 cccgattcgg ggccggcgga tacggaaaaa cccgccaact ggcgggttag gtatagtgtt
    17641 tgggatttgc ctgcctgacc ggggtcttgc gggatggata ttttcgtttt cacgccgtct
    17701 ttttgcgccg tgttgcgtaa tatcctgtcc cgctcgtccg cgccgtaccg gccgcgcacg
    17761 acgttggcga tgatataccg cccgtcttcc gtaacgccaa gcctgccgcc tgccgtgtag
    17821 tcgccgccgt ttgcggttga agccaagtcc cacgcgcgta cccatctgat attcccggca
    17881 ggcaacgcct tgacaaattg caggttgtca ggcttgaacg taccgccgtc aggcggcgca
    17941 ggtttttgca aatactgccc ggcaaacaca tacggcgcgg cctgctccat tcggcgcagt
    18001 gtttcaatat catgcttttc aggccacaac gccgtgccgt cttcctgaat ggcaggcagg
    18061 cacaaatgct cccactcttc gccgttgccg ccgtcaagca gccagcccgc caagtctttc
    18121 tcgtgcaggc gttgcataat caggataatc ggcgtgtcag ggctgttctt ccgggattcg
    18181 accgtgtttt gaaaccagtc gatgacgttc tgccgcctga cctcgcttcg cgcttcatct
    18241 gctttgtgcg ggtcgtcaat gatgatgcag ccgccgaatc cctcccgatg cctgcccgca
    18301 ccgaaacctg taatcgtacc gcccgcacct gttgcgtaca tcacgccgcc tgcggttgtt
    18361 ttccagtgat ggccgctttc gcctgccagt gccaaatcag gaaaaatcgc ccgatactct
    18421 tcgtgttgca ccaagttccg aatctgtacg gagttattga ccgccagcgc cgccgaatag
    18481 ctcgcgtgga taaactcgca atcaggcacg cgccccatcg cccacgcgat aaagttcacg
    18541 accgcgattt ccgttttcga gtatcgcggc ggaatattga taatcaggcg tttcgtttcg
    18601 ccgttgaaaa cacgctcgag cgcgttgcag attggcgcat ggtgattcgc ccgctgccaa
    18661 acatagccgc gcctttgata aaacatccgg cgcgtgaaca tatacaggct tgcagaactt
    18721 aaactgcgaa ttactgatgt ttcaacatcg tcgaattgcc ctagtgccat ttcattctaa
    18781 cttcctttgg agaaacggcc atacagcccc tcttatgatt agcgttttat gctaaaccct
    18841 acgcaacaca tcttccgcta tcttgcgata ttcctcagca tcaagccgta cagccgccct
    18901 catgctccca tcgcccgatt taacgtcaag ctccgtctta tcgccgtatt tcttcggcgc
    18961 aatcttggaa gccgcccact ttcgggcatc tatctgcaat ttcgcctttg aaaccgccgc
    19021 gctctctgct tgtgcactat cggcaatctc gatgatttct tcagcgaaat agtccgcctg
    19081 cttttctctc gcgcgcgcgt attggtggcg aaattcttca ttagcttcca accaacggta
    19141 aatagttttc attggcggca caccatcttc ggcgcatatt gaacgtaggc ttctgccatt
    19201 tgcgattttt tcgcatatct tatcggccat gtctttcgtg taatctgtcg gacggcctaa
    19261 tttgcgtttc gtgccgctca taaaccctcc tcaaaaaaga aaccgtctaa ctctgacccc
    19321 tctcagaatt agacggcaaa cacacactcg ccacatagga aaaacggaac gccgctacct
    19381 gtacaggcag aagctcaaat tcggacagcc taaaacgcaa aaacccgcac attgttatgt
    19441 acgggcttaa aaattcatat ccttcgggcg tgcgaaaatc cccgcaaggg taacgatttg
    19501 aattatacac ctattgccgg aaaaaacaac aggccgtctg aagattcaga cggcatttgt
    19561 attccctact gcatcaaacc gccgacaggt tgcggatttc gggcagtatc gggcggattt
    19621 tttctgcgtg ttccgcgtcg gcgtgtgcgc ttaaggcttc gagggcgttt gcggcggctt
    19681 tgaggcggct gcgcgtttcc gcccagaccg tccacatcgt aaccgcctgc ctgcagccga
    19741 gctgcttcag cggcagggaa acgtctctgc ccatttggat ggcccgcgcg ccgtagctga
    19801 cggcgacggc gaggtcgtac agggcgttgc cgcttatggg cagggcgggt tgcggcgcgg
    19861 gtaacggttc gcggtcgagg acttcgcccg tcaagcccgt gtgcagtgtc aatgcgtgga
    19921 cgtaggcgac ggcttcgggc agcttcccgg cggggatgcc ttcgatggat tcgacgttga
    19981 agcgttggtg tatcatactg tacgcggagg agtagtctat gcctttgcgt ccgacaagcg
    20041 cggcaacggc ttggcgcaat ccggtacggt cgtcggcggt ggttttttgt ccgacttggt
    20101 agccgcctgt tttgcggatg gtgggcagga cttcggatgt tacccatttg cggaatttcc
    20161 aagcggttga gccttgttcc attgctttac ggctgcgaag aatcaaaata tataaaccgc
    20221 tttcgttgat gatgttcacg ttaccgccgc ctccgtgaat ttcagaccgc cctatgttaa
    20281 acatagaccg ctcgtcatca tctaattttt caagtgcttg cgttgggttc tggatttcta
    20341 aagccttaca tacatcggcg gcaacaaacc aagtcaagcc gtctttttca aaggcacgaa
    20401 cgggagaagt agtattgaaa ttaaatgatt gaattacgtt cataatgaag tttcctagtg
    20461 agttttctta atgcccgtta gggcggacgc gtggttaaga accctcacta gatgggcgga
    20521 cttattcccc ttacgggtat tgtattcgtc gcccacgcgt ccataagaaa cttcggttgt
    20581 gccatcgaaa caaacaacac gaaaggaaac ttacagatat gaaaaaatca cattgacgga
    20641 gtgattgccg ctagtgtgtg gttcttacgc cacgaacagg aatataaaac aaacccccct
    20701 gcacatgcaa ggggttttgt taccttattt gggtttgcgt ttaaaattct cgtcatcaca
    20761 acagaatcgg taagaatcaa taaccaacca aacaacgcga agcaggtttt caggcgtatc
    20821 aataataact tgattgcctg acacctcaag tccgcatcgt tcgatattcg aaatatccga
    20881 ctcttccaat tcgataggga agattacaga cggcctttgt ttattgtcaa aataccgtaa
    20941 tatccatctg ttacttttac catcaaccaa tacgccgaaa tagctttccg tatccttcgc
    21001 ctcaatactc gcatcatcag gaagaattga tttaaccaaa tcaaacaacc ttctttctga
    21061 ataagtggta acgattttat tgttttcggg gtcgataatc ggcgcggtcg ggtcttcctg
    21121 ctccttctca acaggggcgg cctcctcctg cactttcggc gcactcagtc ccgatacaac
    21181 cattgaacta accgtatttt gaacagcctg tttgacgata tgacggatgc tttccaaata
    21241 tcgttgggta aactgccgtt gaatattcgc ccttcccgcc acataacgga cgaaatccaa
    21301 atccacctct ttcaagcttt ctgtaattga ttctgtaaat gccgtcaggt atatgctttc
    21361 ttccgcaagg cttcgcaatg catcaggttg aaacttgtcg tgcctgaact gatacagttg
    21421 cgccatatca ttttcattca acatcgtaac atcaacggtc aaaaacggct ccgaatccat
    21481 aatattcttg ttggacaaat cggtaaaaaa tcgccattcc cggccgttgg taatcgcgca
    21541 aatggctatt tcaggcgtgg cattgaagta acgcgacaac tgcggacaat gattggacaa
    21601 attttcagta tatgattttg cttcgataaa cataaccggt gcgccgttgc aaaacaatgc
    21661 gtaatcgacc cgctcccctg attttgcacc tgggaaatcc gcctggtatt ccgcccgaac
    21721 cttattcgga tcgaatgccg aaaaacctag aatatccaat aaaggcaaaa tcaaagcctg
    21781 ttttgtcgtc tcctcggtag tacagatatg tgcaaccttc ttgacatgtt ccgcatggga
    21841 agcaatccgt tctttaaata ccgcacttac agccgccgca ttcatgttaa tctccttggt
    21901 taaatttgat tgggcaaatt cccaccccat taagaagtgt taattttcgc aatcatagcg
    21961 caaccgaaaa gaaaatcaaa ccttctcaaa cagcaaatca aaatcattcc ctgccgcctg
    22021 ccgtatcgcc ctataccacg cggccaagcc caaatccgtc tgcaaatgca ggggcccctc
    22081 gccgcgccgc ctgatttcag cctgcaaacg ttcctcgtag gctgcctgcg acttcgcacc
    22141 gatgccgaac gaaatacgga cggcctcttg ttgcggcgca tccaccttcg cccatgcctg
    22201 caaagtaaga aacatggcat cttcgccgta tctcaagccg atttcaggct tatgcgggca
    22261 agcttcctcc cccatataac ggccttcgat actcaaacat ctatttaggc tgcgcgtatc
    22321 acggtaacga cgctcaaaag cacgcgccaa gtcgttcata aattcaaatt cttgctgatt
    22381 catagcttga ttaacccttt ttcatgcaac aaaaccaaag tccgcattac accttccgcg
    22441 aaggccgtct gaagttcccc ttccgcacaa tccgccaagc ggttctatac atcaaaa
//
LOCUS       DBJGGI010000028        21573 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.028, whole genome shotgun sequence.
ACCESSION   DBJGGI010000028 DBJGGI010000000
VERSION     DBJGGI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21573
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.028"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(317..763)
                     /gene="smpB"
                     /locus_tag="ACSIGJ_001099"
     CDS             complement(317..763)
                     /gene="smpB"
                     /locus_tag="ACSIGJ_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HHQ1329976.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLKQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(818..1828)
                     /gene="waaF"
                     /locus_tag="ACSIGJ_001100"
     CDS             complement(818..1828)
                     /gene="waaF"
                     /locus_tag="ACSIGJ_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HHQ1329977.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRELGRRGYDRVIVLPGSLKSAVIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHQSQEDFDGHSGFPEFSID
                     ERRREISIETFGLNLGKPVLAFCPGAEFGPAKRWPARHFAELGKHYSEAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(1959..2034)
                     /locus_tag="ACSIGJ_001101"
     tRNA            complement(1959..2034)
                     /locus_tag="ACSIGJ_001101"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1999..2001),aa:Asn,seq:gtt)
     gene            complement(2225..2977)
                     /locus_tag="ACSIGJ_001102"
     CDS             complement(2225..2977)
                     /locus_tag="ACSIGJ_001102"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HHQ1329978.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPYPNLTECEAGQF
                     ERDFWDNIGCAPEEYVRIRRAIRLLELRYPDSLNELVCAAIATPLGEMLAVFGVKGLC
                     LLEFVGQKYLEQEIAAVQKALRGRFVFREDGRMQFLRQELDLYFQCRLKTFATPLEMI
                     GTAFQKQAWDVLLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSVMIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            3327..3884
                     /locus_tag="ACSIGJ_001103"
                     /pseudo
     CDS             3327..3884
                     /locus_tag="ACSIGJ_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273197.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
     gene            complement(3862..4677)
                     /locus_tag="ACSIGJ_001104"
     CDS             complement(3862..4677)
                     /locus_tag="ACSIGJ_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHQ1329979.1"
                     /translation="MGALGGIVQHHTALYRSVAVYEPTVVIVRRGCKKLRWAGRELRI
                     AAGEAVGLAGGQTLDVINIPDSDGLYQAQWIAFEQETVERFAAQYGTAQAVCDAVKLP
                     HPGRMGAAFDYAAAVLADEEVPHNAAEAALCGVLAWLQHDGIGFAVYGGVNLMRQIRK
                     LITADMAADWSSAMLAQRLNCSEAALRRRLARQDTNFRTLLTDVRMMRALTLLQVTQW
                     SVAQIAGAVGYDCPSRFSARFKELFGCVPSVVRSEAEPAAYRRTGQVSVGVRP"
     gene            complement(5097..6242)
                     /gene="dapE"
                     /locus_tag="ACSIGJ_001105"
     CDS             complement(5097..6242)
                     /gene="dapE"
                     /locus_tag="ACSIGJ_001105"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HHQ1329980.1"
                     /translation="MTETQSLELAKALISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAVNPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTETGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARTAIAETCGVEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLDDIPKLSAVYERILARLLAEKAV"
     gene            complement(6355..6975)
                     /locus_tag="ACSIGJ_001106"
     CDS             complement(6355..6975)
                     /locus_tag="ACSIGJ_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase"
                     /protein_id="HHQ1329981.1"
                     /translation="MLTPKSCDLFNIPFFQFAQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAADLSEPFAPPHIERWCNGWQVRAHFFAYFKYAQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDDFDTEKYAAFDMWHGAESEYDDYRTVAQQSESDR
                     RLQNDEDFFCIGKHIERDDLGKQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(7009..7518)
                     /locus_tag="ACSIGJ_001107"
     CDS             complement(7009..7518)
                     /locus_tag="ACSIGJ_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HHQ1329982.1"
                     /translation="MNPFETQSVTFAEPIEMLYACHGKVRRFCGQIAMLSGYIAENGC
                     NQLVLQTIRQISRYFNVAAPLHHEDEEENFFPLLLQYAPQAREGVDELLRQHIGLYDN
                     WAAVSAEFAKLEADNAYIPDAEAFKRFVEGYDVHLAIEEPLFDMGKTFIPEEKLTEIG
                     KIMAARRCK"
     gene            complement(7613..8164)
                     /gene="azu"
                     /locus_tag="ACSIGJ_001108"
     CDS             complement(7613..8164)
                     /gene="azu"
                     /locus_tag="ACSIGJ_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HHQ1329983.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAGEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     DYKFACTFPGHGALMNGKVTLVD"
     gene            complement(8447..8869)
                     /locus_tag="ACSIGJ_001109"
     CDS             complement(8447..8869)
                     /locus_tag="ACSIGJ_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HHQ1329984.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFDSAAAKLEERHPELAEALEELVRKYGA"
     gene            9001..11751
                     /gene="secA"
                     /locus_tag="ACSIGJ_001110"
     CDS             9001..11751
                     /gene="secA"
                     /locus_tag="ACSIGJ_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HHQ1329985.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRVLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSETGHEH
                     AEQILTQMGLLAENDSLYSAANISLMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSRLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDIGDLMQEIR
                     SDAVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLQEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQAIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAAVEEQPVGNIQSIHSES
                     PDIEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            11896..13668
                     /gene="dnaG"
                     /locus_tag="ACSIGJ_001111"
     CDS             11896..13668
                     /gene="dnaG"
                     /locus_tag="ACSIGJ_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HHQ1329986.1"
                     /translation="MIPSDFIDELLAKTDIVGIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRHYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLVQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETIDRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            13855..15783
                     /gene="rpoD"
                     /locus_tag="ACSIGJ_001112"
     CDS             13855..15783
                     /gene="rpoD"
                     /locus_tag="ACSIGJ_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HHQ1329987.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKICKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEVSAANLAELKQKVIGHFAQIEKDYKKMIGCLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLQWIEEEIAKGRVWSNALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            complement(15823..16050)
                     /locus_tag="ACSIGJ_001113"
     CDS             complement(15823..16050)
                     /locus_tag="ACSIGJ_001113"
                     /EC_number="3.1.12.1"
                     /inference="COORDINATES: protein motif:HMM:NF014040.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HHQ1329988.1"
                     /translation="MRQISLTDYFCKGLGLRAQTLATIAAVRELLNSGQTPPPDYGKR
                     CKACSLVEVCQPELLEKRDGSVGYVEALFIV"
     gene            complement(16186..16506)
                     /locus_tag="ACSIGJ_001114"
                     /pseudo
     CDS             complement(16186..16506)
                     /locus_tag="ACSIGJ_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
     gene            complement(16467..16823)
                     /gene="cas1"
                     /locus_tag="ACSIGJ_001115"
                     /pseudo
     CDS             complement(16467..16823)
                     /gene="cas1"
                     /locus_tag="ACSIGJ_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762781.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
     gene            complement(16883..17506)
                     /locus_tag="ACSIGJ_001116"
     CDS             complement(16883..17506)
                     /locus_tag="ACSIGJ_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329989.1"
                     /translation="MTQKNDFKNANFYGNTQIVNGDVHNVHTQNTPNSINENEIATYT
                     PEPIWRSPITMAWLTWASFLISLAGLLPIYNCIVKPILYLVNGKTMIQSEINNTYIFI
                     LIAIAVLFLITITLRRITKYQTRYPLVFNYAISGMGHRLSLEKISISQCPQCGGEMKY
                     YNKPIEWENIRYSDGSKKRRVTRTTPALECKRNREHWYKVDPAEDKI"
     gene            complement(17649..18008)
                     /locus_tag="ACSIGJ_001117"
     CDS             complement(17649..18008)
                     /locus_tag="ACSIGJ_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329990.1"
                     /translation="MTRNIEHQFSNLSDVGEKLELENPTVENVVDILVDIGHDDRVYT
                     FHDDFLGLKSGLPQDLLSKHIDELEEGDFADRYSDEIDKILDNANIIFYHLERELSED
                     DLEEIREERERLGLEDD"
     gene            complement(18124..18774)
                     /gene="cas4"
                     /locus_tag="ACSIGJ_001118"
     CDS             complement(18124..18774)
                     /gene="cas4"
                     /locus_tag="ACSIGJ_001118"
                     /EC_number="3.1.12.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HHQ1329991.1"
                     /translation="MTALLTETQRENQDTRLIPLSALQHYAFCPRQCALIHNEQAWAE
                     NYLTAQGKALHERVDSDEPETRKGVRFERTVHVSVEKPGISGILDLVEADTKTGRLKP
                     VEYKRGKPKPDPGDEIQLCAQGLCLEEMTGQTVSEGALWYMQTRHRVPVVFSDGLRAQ
                     TLATIAAVCKLLNSGQTPPPDYGKRCKACSLAEICQPELLGKRDRSVGYVKGLFGE"
     gene            complement(18781..19149)
                     /locus_tag="ACSIGJ_001119"
     CDS             complement(18781..19149)
                     /locus_tag="ACSIGJ_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329992.1"
                     /translation="MKYWLVGASWGGQGHQDQFFVENGYWVLEWESSQQPDQFAKGEK
                     IQVGDRIAIKRMKGQGSSEIKILHIGIVKGVISETNKIICVVDWIVKNLDRNVESRGC
                     FKSIHDPYDKDEWIEKIFCL"
     gene            complement(19176..19936)
                     /gene="cas7c"
                     /locus_tag="ACSIGJ_001120"
                     /pseudo
     CDS             complement(19176..19936)
                     /gene="cas7c"
                     /locus_tag="ACSIGJ_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777774.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
     gene            complement(19956..21242)
                     /gene="cas8c"
                     /locus_tag="ACSIGJ_001121"
                     /pseudo
     CDS             complement(19956..21242)
                     /gene="cas8c"
                     /locus_tag="ACSIGJ_001121"
                     /inference="COORDINATES: protein motif:HMM:NF021243.6"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
     gene            complement(21239..21418)
                     /locus_tag="ACSIGJ_001122"
                     /pseudo
     CDS             complement(21239..21418)
                     /locus_tag="ACSIGJ_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762769.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
     gene            complement(21394..21573)
                     /locus_tag="ACSIGJ_001123"
                     /pseudo
     CDS             complement(21394..21573)
                     /locus_tag="ACSIGJ_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         5428 a         5953 c         5141 g         5051 t
ORIGIN      
        1 ctgaacaaaa aatgtcattt agttcaaatt ttctctattt atttcttatt tattaaaagt
       61 aaacgtttgc tttttgctat tttgtcaagc cggtttgaaa atgtgtataa ttgccctcgt
      121 tatttacaaa aatttcagga aaaatgaccg cactttaccc ttggctaatg ccaatttatc
      181 atcaaattgc tcaaaccttt gacgaaggtt tggggcatca tgctgtgctg attaaagcgg
      241 atgctggttt aagtgtagaa cgtttacgca tcaggcggca gccttgcccg taccgtctga
      301 agcattgttt ccacaatcag cgcgtatgct taatcaaccg ctgtttctcg cgtttccagt
      361 cggcttcctt catactttgt cgtttatcgt gctgtttctt accttttgcc aaaccgattt
      421 ccatcttgat ttttccgcgt gaaaaatgca aatccagcgg cacgatggtg taacctgcac
      481 gttcggtttt tccgattaac ttgttgattt ccgactgttt taataacagt ttgcgcggac
      541 gtacggcatc cggtttgatg tgtgtcgaag ccgtaggcaa agccgtaata tggcagccga
      601 ccagataaaa cgcatctttt ttccaataga tataactctc tttaagctgt acgcgcgcgg
      661 cgcggattgc tttgacttcc cagccttcca agaccaaacc ggcttcaatc cggtcttcaa
      721 tgaaaaaatc gtgaaatgct tttttattgt tcgcaatagc cataaacatc ctatcaatat
      781 ccgccgtcag acggtataaa tccgaaaaca gaagccatca taccgcctct tcaaccgcct
      841 gcacaatctt ctcgggatac agcctgttga ggcagtcggt atgccccaac gggcattcac
      901 gtttgaaaca gggcgaacat tccaagtgca ggctgacgat tttcgccctg tcgctcaaag
      961 gcggcgtatg cgtcgggctg gaagaaccgt aaaccgccac taccttcctg cccaaagcag
     1021 ccgccaaatg catcagcccg ctgtcgttgc acacgaccgt gtccgccaac gacagcaaat
     1081 ccattgcctg cgacaaatcg gttttgccgc acaaattgac acacatgccg tctgaaaggc
     1141 agttgatttc ttcggcgatt tcatcatctt tttgcgaacc gaacaaccaa acctgccaac
     1201 ccgcctccga atagtgtttg cccaactcgg caaaatgcct tgccggccaa cgctttgccg
     1261 gcccgaattc cgcacccggg caaaaagcca gaacaggctt tccaagattc aaaccgaaag
     1321 tttcaataga aatttcccgc cgccgttcat caatggaaaa ctcggggaat cccgaatgcc
     1381 cgtcaaaatc ttcctgactc tgatgcgcga gagccgtata tcggtccacc atcaaaggca
     1441 gacgttcctt atccagcctg cgtatatcgt tcaacagaaa ataacggctt tcgccgacat
     1501 aacccgttct ttttccgatg cccgttgcca gcgcgatgac tgccgacttc aaagaacccg
     1561 gcaacacgat aaccctgtcg tatccgcgcc gtcccaactc cctgcctacc cgccaacggc
     1621 gtttcagctc caacgcaccg tgtccgaacg gattttcaag aatctcattc acttccggca
     1681 tacgctcgaa caccgccatc gaccacttcg gtgcgaacac atcaatcgtg caaccgggat
     1741 gaagttcctt caaacggcgg aacaagggct gggtcatcac gcagtcacct atccaactgg
     1801 gagaaataat caggattttg atagacataa aacgaaaccg aaatcagaca ggcagaattt
     1861 taccgcgaaa ccgttggaaa acctatctcg ccgcattccg aacgccggac gtgcaaatgt
     1921 gaaaaagccc gaacattcaa gttcgggctt caaaattctg gctccccgac ctgggctcga
     1981 accagggacc tgcggattaa cagtccgtcg ctctaccgac tgagctatcg gggaatgggg
     2041 cgtattatag cgtccggaaa aaatgtgtca atccttaatt ttggaaaaat gggcgacaaa
     2101 acgacaagca tatgaatcag aaagacatta agaccgatgc cttaaaagga ttgccgttgt
     2161 atgaattttc acagccgtca tcacaccata tttaagcccg atgagccgtt ctgcatttcc
     2221 ccgcttaaaa caatgccgtc tgaacttcgc cgcgttccaa agccagtaaa aactgtttgc
     2281 ggttcaaccc gcccgcgtag ccggtcagtt tgccgtcgct gccaatgacg cggtggcagg
     2341 gaatcatgac agacaccttg ttctgcccgt tggcggcggc aacggcgcgg acggctttgg
     2401 ggttgcccaa acgctgcgcc tgctccttat aactgcgcgt ttcgccgtaa ggaatcgcca
     2461 aaagcacgtc ccacgcctgt ttttgaaacg ccgtaccaat catttccaag ggcgtggcaa
     2521 aggttttcag acggcattgg aaatacaaat ccaattcctg acgcaaaaat tgcatccgcc
     2581 cgtcttcccg aaacacaaac cgtccgcgca aggctttttg gacggcggca atttcctgtt
     2641 ccaaatactt ctgtccgaca aactccagca ggcacaatcc cttaacgccg aacaccgcca
     2701 acatctcgcc caaaggcgtg gcaatggcgg cacacaccag ctcgttcaag ctgtcgggat
     2761 aacgtaattc caacagacgg atggcgcggc ggatacggac atattcttca ggcgcgcagc
     2821 cgatattgtc ccaaaaatcc cgctcgaact gcccggcttc gcattccgtc agattgggat
     2881 acggcataac gccgcactca aaaacccggg tttcgaggca atggcggatt tcatcccatt
     2941 ttgacggcag attgtttaag gaaggcaggg taatcattcg tttgctccgt atccctatca
     3001 taaatttgac ggcgaaatcc ccgatttccg ccattcccgc acgccggtac aggaacaggc
     3061 tgcggcggaa accttgaggg tctggttgcg gcaatgccta aatatacaat atttccaaag
     3121 catcagggaa aggaatgttt caacacacag gacgacacat aaagcgtcgc cctatgaaaa
     3181 atttcagacg gccttcaaag ggtcgtctga aaccacgatt tttgcatttg cgcattccgg
     3241 cacatcatcc aaccgtttcg gcacattcct gccgccgttg acagcctata atgaatccgc
     3301 ttattcatca agcaaaggaa tcatctatgc aaacccgcat cctctccgcc gtactgctgg
     3361 ctttttcaac cgctgccttt gccgggggcg cattcacgct gcaattcgac aacccgtccg
     3421 aagacggcgg cttcacgcaa aaccagcttt tgagcgcgcc ttacggcttc ggctgttcgg
     3481 gcggcaatgc ttcgcccgcg ctgtcgtgga aaaacccgcc cgcagggaca aaaagtttcg
     3541 tcctgaccgt ttacgataaa gacgcgccga tcggtctgga ccggatgcac tgggtggtcg
     3601 ccgacattcc cgccgatgtc cgccgcctgc ccgcaggcat taccgcacaa tgaggcaggc
     3661 cgcccaaagg cgcgctgcaa acccgcacgg acttcggcgc gccgggctac ggcggcgcgt
     3721 gtcctcccga aggcaggaaa caccgctacg aattcacgct taccgcgctg aaagtcgcca
     3781 agctgccgaa catcaccgcc gaatccacac ccgtgctggt cggtttctcc actaaggcaa
     3841 acagcttggg cgaagcgaaa ttcacggtcg aacaccgacg ctgacctgcc ccgtgcgccg
     3901 atatgccgcc ggctccgctt ccgagcggac caccgacggt acgcaaccga acagctcttt
     3961 gaagcgcgcg ctgaaacggg acgggcagtc atagccaacc gcgccggcaa tctgcgctac
     4021 cgaccattgc gtaacctgca acagcgtcag cgcgcgcatc atgcgtacat ccgtcagcag
     4081 cgtacggaaa ttcgtgtcct gccgcgccaa ccgccgccgc aacgcggcct cgctgcaatt
     4141 aagccgctgc gccagcattg ccgacgacca gtccgcagcc atatcggcag taatcagttt
     4201 gcggatttgc cgcatcaggt tgacgcctcc gtacacggca aaaccgatgc cgtcatgctg
     4261 caaccacgcc aacacgccgc aaagcgcggc ctccgccgca ttgtgcggca cttcttcatc
     4321 cgccagtacc gcagccgcat aatcaaacgc cgcgcccata cgcccggggt gcggcagctt
     4381 caccgcatcg cacactgcct gcgccgtccc gtattgtgcg gcgaaccttt ctacggtttc
     4441 ctgttcgaaa gcaatccatt gcgcctgata gaggccgtct gaatcgggaa tattgatgac
     4501 gtcaagcgtc tgcccgcctg ccaagccgac agcctcaccc gccgcaatcc tcaattcgcg
     4561 ccccgcccac cgcagcttct tgcatccgcg ccgcacgata accacggtcg gttcatatac
     4621 cgccacgctg cggtacaagg cggtatgatg ttgcacgatg ccgcctaaag cacccaatcg
     4681 ttcacgcgta tgaaagtaat ttgacggcac ggttatattc ctcaatgttt caacacacag
     4741 ggccgcacat aaggcgcagc cctgtttacc ctgatttgga aggggttact tcccaaaaaa
     4801 taaaccaaaa ttctactgcc ctgaagggca gggtttcaac catattccgt cggtgtgcct
     4861 gcaacttagc tccctcccct gtggggaggg tcggagggag ggtaaaacgt ggcagataca
     4921 gacaatattt ccgttgccgt cccaatgccc tctccctaac cctctcccac gggagaggga
     4981 atggattacc gttcaaataa atcgctctac ataaaaatca atgcgttatc tcaaactcat
     5041 gttgggtcta atcagattgt cggacatcca tatccgacaa ccagcccatc ttcttttcag
     5101 acggcctttt cagccaacaa ccgcgccaat atcctctcat acaccgccga cagcttcgga
     5161 atatcgtcca gccgcacatt ttcgttgatt tggtggatgg tggcattgga cgggcctaat
     5221 tcgatgagtt ctttcgcaat ggctttaatg aagcgtccgt ccgaagtgcc gccggtggtg
     5281 gacaattcgg cctcaacgcc gcaggtttcg gcaatggcgg tgcgtgccac gtcggtcagt
     5341 ttgcccgcgt gggtcaggaa gggctgtccc gaacacgacc actgcaaatc gtattgcacg
     5401 ccgtgtttgt ccaaaatggc gtggacgcgt tgtttcagcc ctgtttcggt ggactctgtg
     5461 gagaagcgga aattgaattt gacgttcagc tcgcccggaa tgacgttggt cgcgcctgtg
     5521 ccgccgttga tattggaaat ttgaaagctg gtcggcggga agtattcgtt gccttcgtcc
     5581 caaacttcct gcgtcaactc taacaaggcc ggggcaaaag tatgcacggg attcaccgcc
     5641 aaatgcggat aggcgatatg cccttgcttg cctttgacgg tcagattgcc cgacagcgaa
     5701 ccgcgccggc cgtttttaat catatcgccc aatttgtcca cggcggtcgg ttcgccgacg
     5761 atgcagtagt caatcaactc tccgcgtgct ttcaacacat cgacgacttt ggtcgtgccg
     5821 tccagtgcat cgccctcttc gtcggaagta atcaagagtg caatgctgcc ttgatggtcg
     5881 ggatgttcgg caacaaagcg ttcgcaggcg gtaacgaaac aggcgatgct ggttttcatg
     5941 tccgccgcgc cgcgcccgta taatcttccg tcgcgctcgg tcggttcgaa cgggggcgaa
     6001 tcccattttt caacaggacc tgtcggcaca acgtcggtat gccctgcaaa acagacgaca
     6061 ggagctttcg tgccgcgtcg caaccagatg tttttggtgt cgccgaaatg gagttcttca
     6121 gccgcaaaac cgattttgtg caggcgttcg gcaagcagtt tttggcaatc tcgatcgtcg
     6181 ggggtaacgg acgggcggga aatcaacgct ttggcgagtt ctagggattg ggtttcggtc
     6241 attttgttca ctttggaaat caggtcgtct gaaatgtcct gaagctgatt ttcagacgac
     6301 ctccgggttg gattggtgta taagacgggc attcgtaccc gtcagttttc tgaatcattt
     6361 gccgtgacag gcttcgtaaa gcggcagtaa atcttccacc gtttcggcta tccattttgc
     6421 tacatcctgc ttgcccaaat cgtcgcgctc aatgtgtttg ccgatgcaga aaaagtcttc
     6481 gtcgttttgc aaccttcggt cggattcgct ttgttgtgcg acggtgcggt agtcgtcata
     6541 ttcgctttcc gcgccgtgcc acatatcgaa tgcggcgtat ttttcggtat cgaaatcatc
     6601 cagccagcgg ttgtactcgg gcagcgcaat cggcgacacg tcggctttgt agcaatgcca
     6661 gtccaagctg acgctcaggc ggcggcggtt gagcagtatc gacaggatgg cggcggaatt
     6721 tttgtattgg gcgtatttga agtaggcgaa gaaatgggcg cgaacctgcc agccgttaca
     6781 ccagcgttcg atatgcggcg gggcgaaagg ttcgctcaaa tctgcggcga cctgctgtat
     6841 caactgctgc catatctgcc agttttcctt gtagtcggct ttgatttgcg gaatgctctc
     6901 gggctggtat tttttgagct gggcaaactg gaaaaacggg atattgaaca aatcgcagct
     6961 tttgggcgtc agcataatat atccttgaga cgattgtttc agacggcatt atttgcaccg
     7021 gcgcgccgcc atgattttgc cgatttcggt cagcttctct tcggggataa acgttttgcc
     7081 catgtcgaac aaaggctctt caatcgccag atgcacatca tatccctcca caaagcgttt
     7141 gaacgcttcc gcatcgggga tataagcgtt gtctgcttcg agtttggcaa attcggcgga
     7201 aacagccgcc cagttgtcgt acagcccgat atgttggcgc aacagctcgt ccacgccttc
     7261 acgggcttgc ggcgcgtatt gcagcagcag cgggaaaaag ttttcttctt cgtcctcatg
     7321 gtgcagcggc gcggcaacgt tgaaataccg ggagatttgg cggatggttt gcaaaacaag
     7381 ctgattgcag ccgttttcgg cgatatagcc cgacaacata gcaatctggc cgcaaaaacg
     7441 gcgcactttg ccgtggcagg catacagcat ttcaatcggt tcggcaaagg taacgctttg
     7501 ggtttcaaac ggattcatgt tttcgttctc aacggggact tttcaagcag tcattttata
     7561 ataaaacagc ctgcacaaag caggctgtct gtcttttgaa actttaagcg gattaatcga
     7621 ccaaagtcac tttgccgttc atcaaagcac cgtgacccgg gaaggtgcag gcaaatttgt
     7681 agtcgccgtc agccaatttg gcaggatcca aagtcaggga agactcttcg ccgccgccga
     7741 tcagtttggt gtgggcaaca acgcgcgcat cgtcaggttt gacatagtcg gtatcggcag
     7801 cacctacgcc gtctttaaat acgccgtcca tgtcttcagc tttggcaatc acaaggttgt
     7861 gacccatgct ggctttgggt tgcgtaccgg tatgtttcag agtgatggtg aactctttac
     7921 atgctttgct gacttggatg tctttggtgt tgaactgcat attgtcgttg gattcgacag
     7981 ttgccgcaca gttgccggca gcaggggctt cggcagcatc tgcaggagca gcttcggcgg
     8041 caggcgcttc ggaagcgggt gcttcaccgg caggagttgc ctcggcagca ggcgcggcag
     8101 gttcttgaga gcaggcagcc aaaccgataa cggcggcaga aatcagagcc agataagctt
     8161 tcatggtaaa tctccaatcg ataaaataat attcggtttt acggaaatca aagtgcaacc
     8221 gccattaaca aaaccttgaa aaagattccg ccgtgttgca caaacagatg tttcggggcg
     8281 gcattttgct acaaacttca tttgaaatca aagcctgttt gcaagtttac aatcgtttac
     8341 ccaaaaaaag ggcaatctta ccccgaacct atttctttaa tattagggca gccgtccccc
     8401 gccgcgcaaa tattaacgga tatttacaac atccctgccg gccgccttat gccccgtact
     8461 tcctgaccag ctcctccagt gcctccgcca attcgggatg ccgttcttcc aactttgccg
     8521 ccgccgaatc gaaactctcc aacgcagcct tactcaaatg cagggtattg gttttcggcg
     8581 gtttttccgg ttttggaacc agcctgaccg aaacagaccg tatcgaagca tcaagccctg
     8641 ccaactgcgg caataccgac ggggcaatca tcttcaaacg cgatgccgcc atattgtttg
     8701 ccgccaaaag gacaagcctg ccgtcttcga tacatgccgt ctgaaagtgg gggtgcaggt
     8761 tggcaggcag cagttttttc acggcagcat ccaaccgccg ccactgtccc gcctgtttca
     8821 aaagtccgga aagcagcgcg tcccgcctgc ccaactgttc caaattcata aaacatacac
     8881 cccaaaagat tgaaataccg caaacgcgcc tttatttcag acggcattag cactttgcac
     8941 aaacgcttgt gttaaaatcg cgttttcgcc ccactattat atcaggcgca ggaattattc
     9001 atgctgacaa acattgccaa gaaaatcttc ggcagccgca acgaccgctt gctgaaacaa
     9061 taccgtaaat ccgttgccag aatcaacgcg ctcgaagaac agatgcaagc cctaagcgat
     9121 gctgatctgc aagccaaaac tgccgaattc aaacaacgcc tcgccgacgg tcagactttg
     9181 gacggcattt tgcccgaagc cttcgccgtc tgccgcgaag cgtcccgccg cgtactcggt
     9241 atgcgccact tcgacgtgca gcttatcggc ggtatggtgc tgcacgacgg caaaatcgcc
     9301 gaaatgcgta ccggcgaagg caaaaccctg gtcgccaccc tcgccgtcta tctcaacgcg
     9361 ctggccggca aaggcgtgca cgtcgttacc gtcaacgact acctcgcctc gcgcgacgca
     9421 ggcattatgg agccgctcta caatttcctc ggtctgaccg tcggcgtgat tatttcagat
     9481 atgcagccgt tcgaccgtca aaacgcctac gctgccgaca ttacctacgg cacgaacaac
     9541 gaattcggct tcgactacct gcgcgacaat atggtaaccg accaatacga caaagtgcag
     9601 cgcgaattga attttgccgt tgtcgatgaa gtggactcca tcttgattga cgaagcgcgc
     9661 accccgctga ttatctccgg tcaggcagac gacaacatcc agctctacca aatcatgaac
     9721 accgttccgc cccacctcgt ccgtcaagag acagaagaag gcgaaggcga ctattgggtc
     9781 gacgaaaagg cacatcaggt catcctgagc gaaacaggtc acgaacacgc cgaacaaatc
     9841 ctgacccaaa tggggctgct ggcagaaaac gactcgctgt actcggcggc aaatatctcc
     9901 ctgatgcacc accttatggc agcattacgc gcgcactcgc tgttccacaa agaccaacat
     9961 tacgttatcc aagacggcga aatcgtcatc gttgacgaat ttacaggccg tctgatgtcc
    10021 ggccgccgct ggtccgaagg cctgcatcaa gccgtagaag ccaaagaagg cgtggaaatc
    10081 aaacgcgaaa accaaaccct cgcctccatc accttccaaa actatttccg cctgtacacc
    10141 aagctctccg gtatgaccgg tacggcagat accgaggctt tcgagttcca aagcatctac
    10201 aacctcgaaa ccgtcatcat cccgaccaac cgccccgtac agcgcaaaga cttcaacgac
    10261 cagattttcc gttccgccga agaaaaattc gaagccgtcg tcaaagacat cgaggaatgc
    10321 cacaaacgcg ggcagcccgt cctcgtcggt acgaccagca tcgaaaactc cgaactggtc
    10381 tcccgccttc tgcaaaaagc cggactgccg cacaacgtcc tcaacgccaa agaacacgaa
    10441 cgcgaagccc tgattgtcgc ccaagccggc aaagtcggcg caattaccgt cgccaccaat
    10501 atggcgggac gcggtacgga catcgtttta ggcggcaacc tgaagcacca aaccgatgcc
    10561 atccgcgccg acgaaacctt gagcgacgaa gagaaacagg cacaaatcgc cgcactcgaa
    10621 aacggctggc aggcagaaca cgataaagtg atggaagcag gcggtttgca catcatcggt
    10681 acggaacgcc acgaaagccg ccgtatcgac aaccagttgc gcggacgttc cggccgtcag
    10741 ggcgaccccg gatccagccg tttctacctc tcgtttgaag acccgctgtt gcgcctcttc
    10801 gcactcgacc gcgccgccgc catcctcaac cgcctcgccc ccgaacgcgg cgttgccatc
    10861 gagcacaacc tgctgacgcg ccaaatcgaa ggggcgcaac gcaaagtcga aggcagaaac
    10921 ttcgatatgc gcaaacaggt tttggaatac gacgacgttg ccaacgaaca gcgcaaagtc
    10981 atttacagcc agcgcaacga aattctgacc agcaaagaca tcggcgacct gatgcaggaa
    11041 atccgttctg atgccgtcag cgacctcgtg gatacctata tgccgcccga cagcatggaa
    11101 gaacaatggg acatcccgac actggaaaac cgcctggctg ccgaattcag gcttcaggaa
    11161 gacatccaat cctggctgaa ggcggacaat gcgattgacg gtcaagacat caaagagcgc
    11221 ctgatcgaac gtatcgaaaa cgaatatgcc gccaaaaccg aactggtcgg caagcaggca
    11281 atggctgatt tcgagcgcaa cgtgatgttg caggccatcg acaaccaatg gcgcgaacat
    11341 cttgccgcca tggactacct gcgacaaggc atacacctgc gcagctatgc ccagaaaaat
    11401 ccgaagcagg aatataaacg cgaagccttt accatgttcc aagacctgtg gaacggcatc
    11461 aaattccata tcgcctccct gcttacttcg gttcaaatcg aacaaaaccc tgtcgcggcg
    11521 gttgaagagc aacccgtcgg caacatccag tccatccatt ccgaatcgcc cgatatagaa
    11581 gaacttttgg gtcagtcgca aaccgatctg gttaccgaag cctttaatcc cgatgggacg
    11641 gatttcagcc ccgaagcctt ggaagcgcgg ggacaaatcg tccaccgcaa cgacccctgc
    11701 ccctgcggca gcggtttgaa atacaaacaa tgccacggca aattggctta agcgtttgaa
    11761 cgcaaatgcc gtctgaacat ccggtttccg gcttcagacg gcattttgcc tgaaccgcca
    11821 catccgaccg ccattccgaa aaatcccgat ttcgtaccgt ccgtaccgaa aacagacatc
    11881 ccgtcccccc acatcatgat tccatccgac ttcattgacg agcttctggc caaaaccgat
    11941 attgtcggca tcatcgacga gcaggttccg ctgaaaaaag gcggggcgaa ctatatggcg
    12001 tgttgcccgt tccacaagga aaaaacaccg tcgttttcgg tcagtccgac caagcagttt
    12061 tatcattgtt tcagttgcgg ggcgcatggt tcggcgattg gttttgtgat ggaacatcag
    12121 ggactgtcgt ttccggaggc ggttcagttc cttgccgacc gcgtaggtat gattgtgcct
    12181 aaagtgcgcg ggcagaacga taatcccgaa gtccgtgccg agcgcaagaa aaaacagcag
    12241 acgctggaag aaacgacggc ggcggcagct gatttttacg cgcagcagct gaaattcaat
    12301 ccggctgcga aagcttattt ggacaaacgc ggcttgagcg cggaagtcat cgcgcattat
    12361 ggtttgggct acgcgcccga cggctggcag cctttggcgc aagtgttcca accgtatccg
    12421 aataccgcgt tggtggatac ggggatggtg attgacaatg aggggcggca ttacgaccgc
    12481 ttccgccatc ggattatgtt ccccatccgc aatccgcgcg ggcaggtcat cggtttcggc
    12541 ggcagggtgc tggacgactc gaaaccgaaa tatttgaatt cgcccgatac gcctttgttc
    12601 gataagggga aaaaccttta cggcttgtat gaggggcgtg ctgctgtaaa agaggcagaa
    12661 cggattttgg tggtcgaagg ctatatggac gtggtcgcgc tggcacagtt cggcgtgggc
    12721 tacggtgtgg cggccttggg cacggcgacg acggcggaac acgtcaaaat cctgatgcgg
    12781 caggcggaca gtatttattt ctgtttcgac ggcgacagcg cggggcgaaa agcggcttgg
    12841 cgcgcgctgg aaaacgcgct gccgcagttg aaagacgaca aatcgctgca ttttttattc
    12901 ctgccggaag aacacgaccc cgacagctac atccgcgcct acggcaaagc gcaatttgaa
    12961 gacgcgcttc tgaatcaaag caagcctttg tcggaatatt tctgggaaca cctttcagac
    13021 ggcattcatc tcaatacgca ggaaggcaag gcggaattgg tgaaaaccag ttcgccgctt
    13081 ttggtgcaga ttaccgcacc ggcattggct tatttgttaa aacaacggct tagcgagctg
    13141 gtcggcatcg accccgacaa cctcgcgcaa ctgctcggac aggaagcgcc aaagcggcac
    13201 gtcaaacaaa aaaactacaa actgcctccg atttccgtca aacagcccgt tatgccgaca
    13261 ttggtgcaac ggcaaatccg cagcctcttg ataaatccgg attgggctgc atatatagac
    13321 ctgcccgatt atctggcgtt ggacggcgat ttcgcctgcc ttgccaacct cgccgaaacc
    13381 attaaaaacc atccttccgt gccggcaacc gcacaggttt tggaacatat gcgcggttcg
    13441 ccctacgaag aaacaatcga ccgcatcttc cggtcggccc ttcaatcgga agaaatggaa
    13501 ggcggcggcg aagaagattg cgaaaacttc caaatcggca tcaaaaaact gctcaatgag
    13561 ttaaaataca gccaaattga aacattaaaa caaaaaagcc tgcaatccgg cttaaatgaa
    13621 agcgagaaaa aacttttgct gtcgctgctg accgcaaaac aaaattgacc ggcggattcc
    13681 gccatctgta aaccgttatg ccgtctgaaa agcattcacc ccggctgcaa caacgacacc
    13741 tgcagaacac ccatcccaaa aagccttcag acggcatcag agtaccctac tttgccacgc
    13801 cttcaggtgc gtccaaacgc aaaccgtcgg catcttgcca acagaaagca gacaatgtcc
    13861 aaaaaccaaa atcacgaaga ataccaagac gatgcccgcc ccctgacgat tgaggaacaa
    13921 cgtgcgcgcc tgcgccaact catcattatg ggcaaagaac gcggctacat cacctactcc
    13981 gaaatcaacg acgcactgcc cgacgatatg tccgatgccg atcaaatcga caacatcgtc
    14041 agtatgattt ccggtttggg tatccaagtt accgaacacg cccctgatgc ggaagacata
    14101 ttgttaagcg acaatgccgc cgttaccgac gatgatgccg tcgaagaagc cgaggccgcc
    14161 ctttccagcg cagattccga gttcggcagg acgaccgacc ccgtccgtat gtatatgcgc
    14221 gaaatgggac aggtcgacct gctgacccgc gaagacgaaa tcatcatcgc caaaaaaatc
    14281 gaaaacgccc tgaaaaatat ggttcaggcc atctccgcct gcccgggttc cattgcggaa
    14341 atcttagaac tcatcgaaaa aatctgcaaa gacgaaatcc gcgtcgacga agtcgtagaa
    14401 gccattatcg acccgaatga agtattgctc aacgaattgg gcttggggca cttggaaacc
    14461 acagcacccg agaaaccttc caacgacaat tcggacgaaa acgaagacga cgaagaatcg
    14521 gaagaagatg cggatgaagt ctcggcagcc aatctcgccg aattgaaaca aaaagtcatc
    14581 ggccactttg cccaaatcga aaaagactac aaaaaaatga tcggctgttt ggaaaaacac
    14641 cacagccggc acaaagacta tctcgcctac cgcgacgcga ttgccaacaa actgctggaa
    14701 gtccgtttcg ccacccggca aatcgacagc ctcagcagca gcctgcgcgg aaaagtagaa
    14761 aacatccgca aactcgaacg cgaaatccgc gacatctgcc tcgaccgcgt ccatatggaa
    14821 cgcgactact tcatccaaaa cttcctgccc gaaatcacca atctgcaatg gattgaagaa
    14881 gaaatcgcca aaggcagggt ttggagcaac gcactcgacc gcttccgcca cgccatcctc
    14941 gaaaaacaaa ccgagttggc ggatatggaa aaagaaaccc gcatttccat cgaagagttg
    15001 aaagaaatca acaaaaatat ggtgtcgagc gaaaaagaaa ccgcagccgc caaacaggaa
    15061 atgattcagg caaacttgcg cctcgtcatt tccattgcca aaaaatacac caaccgtggc
    15121 ttgcagttcc ttgatttgat tcaggaaggc aacatcggcc tgatgaaggc ggtcgacaag
    15181 ttcgaatacc gccggggcta caaattctcc acctacgcaa cctggtggat ccgccaggca
    15241 attacccgct cgattgccga tcaggcgcgt accatccgta ttccggtaca tatgattgaa
    15301 accatcaaca agatgaaccg catctcgcgc caacaccttc aagaaaccgg cgaagaaccc
    15361 gattccgcca aacttgccga actgatgcag atgcccgaag acaaaatccg caaaatcatg
    15421 aaaatcgcca aagagccgat ttcgatggaa acccccatcg gcgacgacga cgattcgcac
    15481 ttgggcgact tcatcgaaga tgccaacaat gttgcgccgg ccgatgcggc aatgtacacc
    15541 agcctgcacg aagtaaccaa agaaatcctc gaaagcctga caccgcgtga ggcaaaagtc
    15601 ctgcgtatgc gtttcggcat cgatatgaac accgaccaca cgctggaaga agtcggcaga
    15661 cagtttgacg taacgcgcga acgcatccgt caaatcgagg caaaagcact ccgcaagctg
    15721 cggcatccga caagaagcga ccgtttgaga agtttcttgg acagcgaaga cagcaagctg
    15781 taaaccaaaa aaccgcaggt ttcaaatacc tgcggttttt tcttacacaa taaacaacgc
    15841 ttccacatat cccacactcc catcccgttt ctccaacaac tccggctgac agacttccac
    15901 cagagagcag gctttgcagc gtttgccgta gtcgggcggc ggggtttgtc cgctgtttaa
    15961 gagttcgcgg acggcggcta tagttgcgag ggtttgggcg cgtaggccga gacctttgca
    16021 aaaatagtct gttaacgaaa tttgacgcat aaaaatgcgc caaaaaattt tcaattgcct
    16081 aaaaccttcc taatattgag caaaaagtag gaaaaatcag aaaagttttg cattttgaaa
    16141 atgagattga gcataaaatt ttagtaacct atgttattgc aaaggtctca ggccgtctga
    16201 aaacacgacg gggacacggt ggcgggtttg catataccac agcgcgccct cagagacggt
    16261 ttgccccgtc atttcttcca agcacaagcc ttgggcgcaa agctggattt catccaccgg
    16321 gtcaggtttg ggcttgcctc gtttgtattc cacaggtttc agacggcctg tttttgtatc
    16381 catttccacc aaatccaata tgccgctgat gcccggtttt tccgccgaaa cgtgtaccgt
    16441 ccgctcaaaa cgcacgccct tgcacgtttc cggatcgccc gaatctttgc cgcaatgata
    16501 ttgcacgcaa tcggcatggg actttgctcc gacacccgat actgcgcccg acgcaacagc
    16561 acattgccgt tctgccgccc ctgaagccgt cccaaataac acccgttttc ggtaaaaaac
    16621 gccaaattca cattattttc gccgcaaaac cccaacagaa acggcgacac aagcacattc
    16681 ccaaaacaga aaatatgccc gatggaatgc accggtaact gcgccacctt cttacgctcc
    16741 tgctccacca ccagtgtctc ccgctcctta tgcagatagc tgccttgggt ggtgatgtag
    16801 agtgtgtttt gcagtttgcg catgggaaaa ctccttaggt tttgaccgtg cttttggggt
    16861 taaacgaggg gctagctaaa aattagattt tatcttctgc cggatcaact ttataccaat
    16921 gctcacgatt tcttttacac tctaatgctg gtgtagttct tgttaccctt cttttcttgc
    16981 taccatcaga ataacgtata ttctcccact cgattggctt attataatat ttcatttctc
    17041 caccgcactg tggacattga gaaatactaa ttttctctag acttaaacga tgccccatac
    17101 cgctaatagc ataattaaaa actaatgggt atctagtttg atacttagta attctgcgta
    17161 atgtaatagt gataagaaat aatacagcaa tagctattaa aataaatata taagtattat
    17221 ttatttcact ttgtatcata gttttaccat ttactaaata taatatgggt ttaactatac
    17281 aattataaat aggtaatagc cctgctaaag aaatcagaaa actagcccat gtcagccaag
    17341 ccatcgtaat ggggcttctc caaattggtt ccggggtgta tgtggctatt tcgttttcat
    17401 ttattgaatt tggtgtattt tgagtatgta cattatgaac atcaccatta actatttgag
    17461 tattcccata aaaattagca tttttaaaat catttttttg tgtcatgttt ttccttagat
    17521 atcttgaaac ttcaatagaa tattattcag aattaaataa tattaaatat gccctgaggt
    17581 tagctggttg ggtaaaacag cataacccaa cctgtatgta ttgaatctat tctatgacaa
    17641 caagtatact aatcgtcttc taaacccagt ctttctcttt cttctcgtat ttcctctaaa
    17701 tcatcttcac taagctctcg ttcaaggtga tagaatatta tatttgcatt atccagtatt
    17761 ttatcaattt catctgaata tctatctgca aaatcacctt cttcaagctc gtcgatatgt
    17821 ttcgacaata agtcttgtgg caaaccgctt tttaacccga gaaagtcatc atgaaaagta
    17881 taaactcggt catcatgtcc aatatccact aaaatatcta cgacattctc tacagtagga
    17941 ttttctaact ctagtttttc accaacatca gatagattgc taaattgatg ttctatattt
    18001 cttgtcattt acgactcctt aaaatgtgta attacaattg agcatgattg ctcgttttca
    18061 gaaattgcga ttaatttgga agattcaagt taattaacgt aataattttt ttcaaataaa
    18121 atattactcc ccaaacaacc ccttcacata tcccacactc ctatcccgtt ttcccagcaa
    18181 ctccggctgg caaatctccg ccagcgagca ggctttgcag cgtttgccgt agtcgggcgg
    18241 cggggtttgg ccgctgttta ggagtttgca cacagcggcg atggtggcga gggtttgggc
    18301 gcgcagaccg tctgaaaaca caacggggac gcggtggcgg gtttgcatat accacagcgc
    18361 gccctctgaa acggtttgcc ccgtcatttc ttccaaacac aaaccttggg cgcaaagctg
    18421 gatttcatcc cccgggtcag gtttgggctt gcctcgtttg tattccacag gtttcagacg
    18481 gcctgttttc gtatccgctt ccaccaaatc caatatgccg ctgatgcccg gtttttctac
    18541 cgaaacgtgt accgtccgct caaaacgtac gcccttgcgc gtttccggct cgtccgaatc
    18601 cacccgctca tgtaacgctt tgccttgcgc ggtcaaatag ttctccgccc acgcctgttc
    18661 gttgtgaatc aaagcacatt gacgcgggca gaaggcgtag tgttgcaggg cggaaagggg
    18721 aatcaggcgc gtatcctgat tttccctttg ggtttcggtt aaaagtgcgg tcatcttttg
    18781 ctacaaacaa aaaatttttt ctatccattc atctttatca tatggatcat gaatactttt
    18841 aaagcatcct ctactttcaa catttctatc taaattttta actatccaat caacgacaca
    18901 gataattttg ttcgtttcag aaataacccc cttaacgatt ccaatatgta aaatcttaat
    18961 ttcagacgat ccttgacctt tcatgcgttt gattgcaatt cgatcaccta cttggatttt
    19021 ttctcctttt gcaaattgat ctggttgttg agaggattcc cactccaaaa cccagtaacc
    19081 attttcaaca aaaaattgat cttgatgccc ctgccctccc caagaggctc caactagcca
    19141 atatttcata tcgttactcc tgatttattg aaaacttaca aatactcttc cacactcacg
    19201 ccatttagcc cgtctgaaac cacgctgatt ttgtaatcgc caaaaccgct tgcgggcgta
    19261 cctgattcgc cgtttacgcg ttcgactttc acggcgtcaa acagtttatg tgcaggctgg
    19321 ctgccgagtg cgctgtcgtg tttgaaaaca accagtttgc gtgccgccat ttcgccacgg
    19381 gcggcggagc ggtcgtgttc aaacatcaac gtcagggctt gccagagttt ggctaagtcg
    19441 tcgtctgaaa aaccggtttt ggcggcaagg ttggcggaga taaagccatg cacgcggtag
    19501 agcgcgtaag ggacgatgta tttgcgcccc atggtgcgtt ctttttccaa gtctttttca
    19561 ttggttaccg ccatgcgggt gatggaaact tccggcggca caatcgggtc gatggattgg
    19621 gcaaacgcca gttgtaccgg gccgcgcact tgtccgctgt tgacttcggt ggtcatcacg
    19681 gcgccgaagg tgcggatgtc aaagaagttt ttacacatcc aagcggtaat atcacgggct
    19741 ttggcttcgt ccttcggcag tttttggctt ccgactcaat gccaagcgcc tcataagcgc
    19801 gtttgttttg caggttcagc acgctttttt ctttgacgta gatttcgtaa ccggcttcgt
    19861 tttcgctgct gatttcgaca aaattacgga ttttgcgttt caggcagaca tcggttacca
    19921 agcctttgga tgttttcgct tttgcccagc acggcagttt gcaacaagag ccgccaaata
    19981 gacggcggcg ttttccacgc acaaggctca agggctaaat cttgccaatg atgcgccaag
    20041 ttttccgcca gctgcccaaa tgtggtgtcc agccaaaacc gaacagaaat ccgcgcagca
    20101 ttgggggcaa gccccaagat gtaaaaacgg gtattgggag agagttcagg cgcaatttct
    20161 tgcagcggac gacctttgcc gatttgttcc aatacgttga aaattttggc gctttcttgt
    20221 tcgtcatccg acggcgtaaa cacttgcgcg aagaagcctt cggcagcctg cgccgtggcg
    20281 ttatagtgga ttaaatttaa accggtacgg cgttgcctcg ccttgccgta ctatttgtac
    20341 tgtctgcggt ttcgtcgcct tgtcctgatt taaatttaat ccactatatc atccgcttcc
    20401 gcccaaaaga ccgtgctggc atcgcctatg gtcaggcagt gattattttc gcggcgcaag
    20461 agatagttca gcgcggtggt gtaggcaaag gcggattgtt cggaaacagg cgcatttgcg
    20521 ccttgctctt ttccgaaaga ggaaaaggct tctttattaa acgaaataat cgaaccgccg
    20581 gagctttgcc cgccaaacac gcctttaatc gccggatgca gccgtgcaat cggcgcggtg
    20641 tcgccgctaa tcaggtataa gctttcgagt gcttcatcac ttttcaggca gcctgcccac
    20701 aaggtttgcg cggcttcgcg tttatggata agagcggtcg gtttttcaag agaaaatgcg
    20761 gtgttggaat caagcatttc ggcaggcagg ttttcggcag cgaaatgtgc aggctgccag
    20821 ttttgcagaa aacggcataa ggcttgtaaa ccttcgtctt cgctgttttg cagtaaatcg
    20881 agatggtatt gcttgaaggc ttcaaaggtt ttttcagacg gcgtaaacgg tttttctttg
    20941 gcttcggctt tgtttttatt ggcttccacg ccaagcgcgt aggcggtttt atcccacaaa
    21001 aaattcggtt tgatacccga cgtgcgtttt tcagggcgcg gcacactcat cagcttcggc
    21061 tgcggctttt tatcggcagt cagattcggc acaacggttt tcagacgacc ttctttatcc
    21121 aacaccaaaa tccagccgat tttctcctcg ctaaaaccat aagacggcac tttcgggttg
    21181 cccgtttcat cggtttccgt tgccaaacgg cggtaatagc ggacaaggga cgcaagaatc
    21241 atgctttcac ctcctcggcg taaaacggcg gcacatcaat caagccgtct ttcatttgcg
    21301 tgcggaaaaa atgcggcgtg ttgccgtgat caaaatcaat atcgtgcagc atccagccca
    21361 aatcgcggtt tgcctcgctt tccgataacg ccgacggcgg cagcggttca cccttatccg
    21421 cgctttcgcc atttgccctt tcaggctgcg ggcatagggg cggaacaggc ggcggtcgaa
    21481 tcctgtttca tccggacaaa cgcgttggca gtcggaaaat ccggccggcc gtgtcaaata
    21541 atgcgttact ttggccgggt cttgtccttt gta
//
LOCUS       DBJGGI010000029        21567 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.029, whole genome shotgun sequence.
ACCESSION   DBJGGI010000029 DBJGGI010000000
VERSION     DBJGGI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21567
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.029"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..1588
                     /locus_tag="ACSIGJ_001124"
     CDS             <1..1588
                     /locus_tag="ACSIGJ_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HHQ1329993.1"
                     /translation="WPGVFSHDLWNPAEPAVYTAVEALAGSPTPLVAHLFGQTDFGIP
                     PVYLWVAAAFKHLLSPWAADPYDAARFAGVFFAVIGLTSCGFAGFNFLGRHHGRSVVL
                     IHIGCIGLIPVAHFLNPAAAAFAAAGLVLHGYSLARRRVIAASFLLGTGWTLMSLAAA
                     YPAAFALMLPLPVLMFFRPWQSRRLMLTAVASLAFALPLMTVYPLLLAKTQPALFAQW
                     LNYHVFGTFGGVRHIQTAFSLFYYLKNLLWFALPALPLAVWTVCRTRLFSTDWGILGI
                     VWMLAVLVLLAFNPQRFQDNLVWLLPPLALFGAAQLDSLRRGAAAFVNWFGIMAFGLF
                     AVFLWTGFFAMNYGWPAKLAERAAYFSPYYVPDIDPIPMAVAVLFTPLWLWAITRKNI
                     RGRQAVTNWAAGVTLTWALLMTLFLPWLDAAKSHAPVVRSMEASFSPELKRELSDGIE
                     CIGIGGGDLHTRIVWTQYGTLPHRVGDVRCRYRIVRLPQNADAPQGWQTVWQGARPRN
                     KDSKFALIRETGENILKTTD"
     gene            1637..1750
                     /locus_tag="ACSIGJ_001125"
                     /pseudo
     CDS             1637..1750
                     /locus_tag="ACSIGJ_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1795..2400
                     /locus_tag="ACSIGJ_001126"
     CDS             1795..2400
                     /locus_tag="ACSIGJ_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HHQ1329994.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDIDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAAANKEQAKAREAIGNGLTMLSPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            2472..2864
                     /locus_tag="ACSIGJ_001127"
     CDS             2472..2864
                     /locus_tag="ACSIGJ_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HHQ1329995.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLRIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     EYRPDTPSENTSAETAPRPAKKGLKLVK"
     gene            2869..3015
                     /locus_tag="ACSIGJ_001128"
     CDS             2869..3015
                     /locus_tag="ACSIGJ_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1329996.1"
                     /translation="MPSERNRVSDGILSDGAQTESVYRQNLFRRIETVLPLPIRYWDI
                     ENAV"
     gene            complement(3063..3509)
                     /locus_tag="ACSIGJ_001129"
     CDS             complement(3063..3509)
                     /locus_tag="ACSIGJ_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HHQ1329997.1"
                     /translation="MSLKTRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDGAKIYTEAGRQDLADKENAEIDVLHRYLPQMLSAGEIRTA
                     VEAAVAETGAAGMADMGKVMVVLKTRLAGKADMGEVNKILKTVLTA"
     gene            complement(3641..3853)
                     /gene="rpsU"
                     /locus_tag="ACSIGJ_001130"
     CDS             complement(3641..3853)
                     /gene="rpsU"
                     /locus_tag="ACSIGJ_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HHQ1329998.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(4142..5992)
                     /gene="ltgA"
                     /locus_tag="ACSIGJ_001131"
     CDS             complement(4142..5992)
                     /gene="ltgA"
                     /locus_tag="ACSIGJ_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase LtgA"
                     /protein_id="HHQ1329999.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQNNDAAAAAYLENAGDSAMAENVRNEWLKS
                     LGARRQWTLFAQEYAKLKPEGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSPEQRSFAWGVLGHYQSQSLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDADTNSVLRMAEDGAIKRALVLFQHSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLAAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEILATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMTNAAYYASLFGAPHIPLKQRMGTVPAR"
     gene            6369..7106
                     /locus_tag="ACSIGJ_001132"
     CDS             6369..7106
                     /locus_tag="ACSIGJ_001132"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HHQ1330000.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIQDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDTGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLSNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHAQSEIGRELIRED"
     gene            7108..7794
                     /locus_tag="ACSIGJ_001133"
     CDS             7108..7794
                     /locus_tag="ACSIGJ_001133"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HHQ1330001.1"
                     /translation="MADLTFEQAVSTIVGMKDEIVRALGETFVMVGLSTTFAVIFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVLKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            7953..8819
                     /locus_tag="ACSIGJ_001134"
     CDS             7953..8819
                     /locus_tag="ACSIGJ_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HHQ1330002.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASAAAPSADNGAAKK
                     EIVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHK
                     PYLDDFKKEHNLDITEAFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARALV
                     MLNELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYA
                     ISSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGY
                     KYPAAWNEGAAK"
     gene            complement(8882..9451)
                     /locus_tag="ACSIGJ_001135"
     CDS             complement(8882..9451)
                     /locus_tag="ACSIGJ_001135"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HHQ1330003.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDVETHLVVSKGAEMAR
                     ASETDYTKDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSIAHTLSLFGIDTPDLAEWQGMAD"
     gene            9609..10469
                     /locus_tag="ACSIGJ_001136"
     CDS             9609..10469
                     /locus_tag="ACSIGJ_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HHQ1330004.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTAVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLSEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPESVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGKIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            10605..10781
                     /locus_tag="ACSIGJ_001137"
     CDS             10605..10781
                     /locus_tag="ACSIGJ_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330005.1"
                     /translation="MNTRINFTATVGKRVSQCYVTSVISTIGVTTSDAICSGGGTNKA
                     KSQCNALLKAAGRC"
     gene            11144..11497
                     /locus_tag="ACSIGJ_001138"
     CDS             11144..11497
                     /locus_tag="ACSIGJ_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HHQ1330006.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFVPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            11487..12353
                     /gene="atpB"
                     /locus_tag="ACSIGJ_001139"
     CDS             11487..12353
                     /gene="atpB"
                     /locus_tag="ACSIGJ_001139"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HHQ1330007.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHSEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRIVPTADLNTALALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            12410..12646
                     /gene="atpE"
                     /locus_tag="ACSIGJ_001140"
     CDS             12410..12646
                     /gene="atpE"
                     /locus_tag="ACSIGJ_001140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HHQ1330008.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            12716..13186
                     /locus_tag="ACSIGJ_001141"
     CDS             12716..13186
                     /locus_tag="ACSIGJ_001141"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HHQ1330009.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKIAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLRDQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            13191..13724
                     /locus_tag="ACSIGJ_001142"
     CDS             13191..13724
                     /locus_tag="ACSIGJ_001142"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HHQ1330010.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            13735..15282
                     /gene="atpA"
                     /locus_tag="ACSIGJ_001143"
     CDS             13735..15282
                     /gene="atpA"
                     /locus_tag="ACSIGJ_001143"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HHQ1330011.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVSTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTLGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPVEKIAPGVIARKSVDQPMQTGLKAIDSMIPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            15307..16182
                     /gene="atpG"
                     /locus_tag="ACSIGJ_001144"
     CDS             15307..16182
                     /gene="atpG"
                     /locus_tag="ACSIGJ_001144"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HHQ1330012.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            16220..17617
                     /gene="atpD"
                     /locus_tag="ACSIGJ_001145"
     CDS             16220..17617
                     /gene="atpD"
                     /locus_tag="ACSIGJ_001145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HHQ1330013.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGSPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFSGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LAVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            17628..18050
                     /locus_tag="ACSIGJ_001146"
     CDS             17628..18050
                     /locus_tag="ACSIGJ_001146"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HHQ1330014.1"
                     /translation="MSIMQVEVVSGEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGILEVQPDKVTVLADVAVRSAEMDRARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            18222..19118
                     /gene="glyQ"
                     /locus_tag="ACSIGJ_001147"
     CDS             18222..19118
                     /gene="glyQ"
                     /locus_tag="ACSIGJ_001147"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HHQ1330015.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLFAEENADLALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYIESREKLGFPLIKK"
     gene            19235..21298
                     /gene="glyS"
                     /locus_tag="ACSIGJ_001148"
     CDS             19235..21298
                     /gene="glyS"
                     /locus_tag="ACSIGJ_001148"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HHQ1330016.1"
                     /translation="MTTQTLLIELLTEELPPKALNNLGNHFAASVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGTPTKALEGFARGAGAK
                     IEDLTIIHDGRQDVYAYEYVQTGRPLGGLLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDVVNVSVLGLQSGNQTLGHRFLSDGEMIIENADSYAAQMRGQGKVVA
                     SFAGRKAAIQTALEGQARRLNATVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPQGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     VFKQLPEAAALAAANKRVQNLLKKADAALGEVNESLLQQDEEKALYAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAGQMNAVADI
                     ALLGE"
     gene            21362..>21567
                     /locus_tag="ACSIGJ_001149"
     CDS             21362..>21567
                     /locus_tag="ACSIGJ_001149"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HHQ1330017.1"
                     /translation="MQPLVSVLICAYNVEKYFAQSLAAVVNQTWRNLDILIVDDGSTD
                     GTLAIAKDFQKRDSRIKILAQAQNS"
BASE COUNT         4989 a         5789 c         5721 g         5068 t
ORIGIN      
        1 gtggcccggc gtgttttccc acgatttgtg gaatcctgcc gaacctgccg tctataccgc
       61 cgtcgaagca ctggcaggca gccccacccc cttggttgcc catctgttcg gtcaaaccga
      121 tttcggcata ccgcccgtgt atctttgggt tgccgccgca ttcaaacatt tgctgtcgcc
      181 gtgggcagcc gacccgtatg atgccgcacg ctttgcaggc gtattttttg ccgttatcgg
      241 actgacttct tgcggctttg ccggtttcaa ctttttgggc agacaccacg ggcgcagcgt
      301 tgttttaatc catatcggct gtatcgggct gattccggtt gcccatttcc tcaatcccgc
      361 cgccgccgcc tttgccgccg ccggactggt gctgcacggc tactcgctgg cacgccggcg
      421 cgtgattgcc gcctctttcc tgctcggtac gggttggacg ttgatgtcgc tggcggcagc
      481 ttatccggcg gcgtttgcgc tgatgctgcc cctgcccgtg ctgatgtttt tccgtccgtg
      541 gcaaagcagg cgtttgatgt tgacggcagt cgcctcgctt gcctttgccc tgccgcttat
      601 gaccgtttac ccgctgctct tggcaaaaac gcagcccgcg ctgtttgcgc aatggctcaa
      661 ctatcacgtt ttcggtacgt tcggcggcgt gcggcacatt cagacggcat tcagtttgtt
      721 ttactatctg aaaaatctgc tttggttcgc actgcccgcg ctgccgctgg cggtttggac
      781 ggtttgccgc acacgcctgt tttcgaccga ctgggggatt ttgggcattg tctggatgct
      841 tgccgttttg gtgctgctcg cctttaatcc gcagcgtttt caagacaacc tcgtctggct
      901 gctgccgccg cttgccctgt tcggcgcggc gcaactggac agcctgaggc gcggcgcggc
      961 ggcttttgtc aactggttcg gcattatggc gttcgggctg tttgccgtgt tcctgtggac
     1021 gggctttttc gccatgaatt acggctggcc cgccaagctt gccgaacgcg ccgcctactt
     1081 cagcccgtat tacgttcccg acatcgatcc cattccgatg gcggttgccg tactgttcac
     1141 acccttgtgg ctgtgggcga ttacccggaa aaacatacgc ggcaggcagg cggttaccaa
     1201 ctgggcggca ggcgttaccc tgacctgggc tttgctgatg acgctgttcc tgccgtggct
     1261 ggacgcggcg aaaagccacg cgcccgtcgt ccggagtatg gaggcatcgt tttccccgga
     1321 attaaaacgg gagctttcag acggcatcga gtgtatcggc ataggcggcg gcgacctgca
     1381 cacgcggatt gtttggacgc agtacggcac attgccgcac cgcgtcggcg atgtccgttg
     1441 ccgctaccgt atcgtccgcc tgccccaaaa cgcggatgcg ccgcaaggct ggcagacggt
     1501 ctggcagggt gcgcgcccgc gcaacaaaga cagtaagttt gcactgatac gggaaaccgg
     1561 ggaaaatata ttaaaaacaa cagattgagc cgaatttctg gattaagtgc cggaaatcgc
     1621 gtataattgc gcgattaaac ctttatatag tggattaaat ttaaatcagg acaaggcgac
     1681 gaagccgcag acagtacaaa tagtacggca aggcgaggca acgccgtacc ggtttaaatt
     1741 taatccacta taaatcagcc gttttgcagg catcacacag gagcgacaat tattatgatg
     1801 accctctatt ccggcattac ctgccccttc agccaccgct gccgcttcgt tttgtacgaa
     1861 aaaggtatgg attttgaaat caaagacatc gatatttaca acaagcccga agacctcgcc
     1921 gtcatgaacc cgtacaacca agtccccgtg ctggtcgagc gcgatttggt gctgcacgag
     1981 tccaacatca tcaacgaata catcgacgaa cgcttccccc atccgcagct tatgcccggc
     2041 gatcccgtta tgcgcggccg ggggcggctg gtgctgtacc gtatggaaaa agaattgttc
     2101 aaccacgtcc aagttttgga aaaccccgcc gccgccaaca aggaacaggc aaaagcgcgc
     2161 gaagccatcg gcaacggcct gacgatgctt tccccttcgt tcagcaaaag caaatacatc
     2221 ctcggcgaag atttttctat gattgatgtt gccctggccc cgctgttgtg gcggctcgac
     2281 cattacgatg tcaaactggg caaaagtgcc gcgccgctgc tcaaatacgc cgagcgtatc
     2341 ttccagcgcg aagcctttat cgaagcactg acacccgccg aaaaagccat gcgcaaataa
     2401 gtccgaaatg cttgcaaaac ccgccgtttt gcaggcattt tcacattcag gcacaccaca
     2461 cggaacccat catgcccacc tcaaccaaac cctacatcct ccgcgcactt tgcgaatggt
     2521 gcagcgacaa cagcctcacg ccgcacatcc ttgtctgggt aaacgagcat acgcgcgtcc
     2581 ccatgcagta cgtccgcgac aacgaaatta tgctcaacat cggcgcaacc gccacgcaaa
     2641 accttcgaat cgacaacgat tggatcagct tttccgcccg tttcggcgga caagcgcacg
     2701 acatatggat acctgtcgga cacgtcctca gccttttcgc acgggagacc ggagaaggta
     2761 tggggtttga attggaggag taccgccccg acacgccgtc tgaaaacact tctgccgaaa
     2821 ccgcgccccg gcccgccaaa aaaggtttga aattggtcaa ataaatctat gccgtctgaa
     2881 cggaatcgtg tttcagacgg cattttgtcc gatggggcgc aaacggaatc cgtttatcgg
     2941 caaaacctgt ttcggcgtat cgaaaccgtg ttgcccctgc cgattcgata ttgggacatt
     3001 gaaaatgccg tctgaacctg cgatacgggc ttcagacggc gttttgtccg atattcgggc
     3061 aatcaggcgg tcagtacggt tttcaagatt ttgttgactt cgcccatatc ggctttgccg
     3121 gcgaggcggg ttttcaatac gaccatcact ttgcccatat ccgccatacc tgccgcgccg
     3181 gtttcggcaa cggctgcttc gacggcggtg cggatttcgc cggcggagag catttgcggc
     3241 aggtagcggt gcagcacgtc gatttcggcg ttttctttgt ctgccaaatc ctgacggccg
     3301 gcttcagtgt agattttcgc gccgtctttg cgctgtttga ccattttggt caggatggcg
     3361 gtgattttgg catcgtcggc ttcggtgcgt tcgtctactt caaactgttt gacggcggca
     3421 ttgatgaggc ggatggtgcc gagggaaact tgatctttgg cgcgcatcgc ggttttcata
     3481 tcttcggtaa ggcgggtttt caggctcatg atgtcctcgc tgggatgtcg gatggaaacg
     3541 gcgggtttcg atgccgtctg aaaagcaaaa cacaccgcag agcaggtctt gcggtgtgtc
     3601 ttcatatcac agggctgacc tgtaatctgt acttgaacgt ttagtacatt ttgggcggca
     3661 gctgttggct gcgcaggcgt ttttgcaggc gttttacggc tgccgctttt ttgcgtttgc
     3721 gttcggtagt cggtttttcg taggcttcgc gggcgcgcag ctcggtcagc aggccggttt
     3781 tttctacggc gcgtttgaaa cggcgcatag cgacttcaaa tggttcattc tcttttacgc
     3841 ggattgcagg cattttattt cctttaataa attcggttgt tccatctgcc catcatatcg
     3901 gtggaaaggg tagggcagac ggtgttgtag gttttccgta tcgctgtgcc tttcggcggc
     3961 gggatacgcg ccgtgacgga aacatcacct cctaacgggc cggcttgtgc cgtgtcaagc
     4021 tgcttgaaaa agtaggaaaa acaattttcg gattgtctta tatttatggt tcgccgtcaa
     4081 tgccgttcgg gataaaaatg ccgtctgaaa gaccgggcgg gtttcagacg gcatcggcac
     4141 gtcagcgtgc gggaacggtg cccatacgct gtttgagcgg gatgtgcggc gcgccgaaga
     4201 gggaggcgta gtaggcggca ttggtcatca cttttttgac atagtcgcgc gtttcggaaa
     4261 acgggatggt ttcggcatat accgcgcctt cgaggggtgt gtccgcctgc catcggcgcg
     4321 ctctgccggg gccggcgtta tagcctgcgg tggcgaggat ttcgttgttt tgcaggcggc
     4381 gtttggtgtc cgccatatac cacgtcccca tacggatatt gccgtcggcg gtgtagagct
     4441 gggcggcatc cataccgatt ttgccggcga tttcgcgcgc ggtggcaggc ataacctgca
     4501 tcagcccctg cgcgcctacg cgggattgcg cgcctataac gaagcggctt tcctgacgaa
     4561 tcaggccgta aacccaagcc ggatcgacat taacattttg cgcgtggcgg attacggtgt
     4621 ctttaaacgg cgaaatgtag cgcaaggtgt agttgagttt gcggtcggtg cgttccgcgc
     4681 tgttgaccgc catatcgtaa aaaccgtggt cgaacgcggt ttgcgcggcg gccagcagct
     4741 tgtcttcgtc aaagccgcgt gtggcaaaac gccattccgc ctgagcctga cggcgcattt
     4801 tcgcatcgcc ggcggttcgg ctgtgttgga acaacaccag tgcgcgctta atcgcgccgt
     4861 cttccgccat acggaggacg ctgtttgtgt cggcatcggg aacattgttg cgcgtatcga
     4921 ttttgcgccc caactcttcc cctgccagta cggcataaaa attcctgccc gtcgctgccg
     4981 cctgtttgta aagtttttcc gcttcttgcg tgttgcccgt tgcggcgcgg ctgcgtgcca
     5041 gccagtagag ccaggtcggg cttttttgca gtttttcggg catatgcgag atcacggagg
     5101 ccagctcgtc ccaacgtcgg gcgcgcaagg cggcgcgggc gtaccactcg atttggtcgt
     5161 cggtcagttg gcggcggtcg gcaaccttgc cgtaatagtc caaggcggca ggcacattga
     5221 ggctttgcga ctgataatgc cccaatacgc cccacgcgaa actgcgttgt tcggggctta
     5281 aaccgctttc catttcggac agcagggcgg cggcattcgg cgatttgcgt gcttctttgc
     5341 cgatgacgtt caacagggca tattcgcgcg aaccttgtgt accgccgtca aacgggctgc
     5401 ccaatgcggc ggcaaggttg cgtgcgtctg tggtttggcg gccggccagc agtccgcgca
     5461 cgcgcctcca ggcatcgttg ccgtccaaca agccggatgc ggctgcctgt tccaacagtt
     5521 tggtgcagcc cgaaggcagt ttgcccgtat ttttaactag ttcggcggca cgcgtatagt
     5581 cgttgcggct cgaatcggcg tagcattcga cttcttgggc gcgcccttca ggtttgagtt
     5641 tggcatattc ctgtgcaaac agcgtccact gtctgcgcgc gcccaaagac ttcagccact
     5701 cgttgcggac attttccgcc atcgcgctgt cgccggcgtt ttccaaatag gcggcggctg
     5761 ccgcatcgtt gttctgtttc actgcatcca gtgcggacgg gtagccgccg taatctgcca
     5821 gcgtttttcc ttccggttcg gcagggcggg tgggaacgct tgccgaaagg tcggcagttt
     5881 ctatattgtc tgccggggtc ttgccggctg gcagtgtgtt tgtcgaagaa cacgcggcaa
     5941 gcaccagggc cgccagcagc ggcagggaat gcttcataga gggtaggtac atcggatttc
     6001 cttaagaatc ggaaccctga acggtcaggg ttggaaaaga caaaatgccg tctgaacagg
     6061 cgtttgcccg aattatatgc cgaaactgca ccgtctttgg aatgtttccg acataattta
     6121 tatttttcaa tcatttgccg tttgggtgcg aaccgctgcc tttgcccgtt tcagacggca
     6181 ttgtccgaaa tggttgcccg cttcctgcct tattgacaaa aaaacgcttt cccgataata
     6241 tcctacgaaa attaacctgc cgatttgaca cagcttgcgg gcataacagc taaagcgttc
     6301 cgacaatttc agctttatct tccgcgcccg ttgtgtccga catcgggctt tgttgtatgg
     6361 gaaagacaat gattattttg gacaaggttt ccaagcatta ccaaacgcgc gacaaaaccc
     6421 gttttgccgc cgtcgagccg accagcctcg aaattcaaga cggcgaaatc ttcgggctga
     6481 tgggctattc cggcgcgggc aaatccactt tgttgcgcct gatcaacctg ttggaacgcc
     6541 ccgacacggg caaagtcaac gtctgcggac aagaactgac cgcgctcgat gccgccgcat
     6601 tgcgtcaggc gcggcagaat atcggcatgg tgtttcagca gttcaacctt ttaagcaacc
     6661 gcaccgtcgc cggcaacgtt gcctttccac tggaaatcgc cggatggccg tctgaaaaaa
     6721 tcaaagcgcg cgttgccgag tgcctcgaaa tcgtcggctt gaccgaacgc gccggccact
     6781 accccgccca gctttcaggc gggcagaaac aacgcgtcgg aatcgcccgc gcgctcgcgc
     6841 ccaagccgca agtcatcctc gccgacgaac ccacttccgc cctcgacccc gccaccacgc
     6901 gcagtgtctt ggaatgtttg gaagacatca acaaacgctt caacgttacc atcgtcatcg
     6961 taacccacga aatgagcgtc atcagacgat tgtgcgaccg cgccgccctg ttggataaag
     7021 ggaaagtggt ggaaatcgtc gaagtacgcg gcaaccaaat ccacgctcaa tccgaaatcg
     7081 ggcgcgaact gattcgggag gactgatatg gcagacttaa cattcgaaca agccgtttcc
     7141 accatcgtcg gcatgaaaga cgaaatcgtc cgcgccttgg gcgaaacttt cgtgatggtc
     7201 ggcttgtcca ccacattcgc cgtcatcttc ggcacgctct tgggcgtgtt gctcttcgta
     7261 acttccagcc gccaactgca ttacaacaag ccggtgaact tcctgctcga caacctcgtc
     7321 aacctcatgc gcgccttccc cttcgtcatc ctgatgattg cgatgatacc cgccacacgc
     7381 gccatcgtcg gcagcaccat cggcccggtt gccgcctcgc tggtgttgag cgtgtcggga
     7441 ttgttttatt ttgcccgact ggtggaacaa aacctgcgcg aagtcccaaa aggtgtgatt
     7501 gaagccgccg ccgcgatggg tgcgccgccg attgccatcg tcctcaaagt cctcttgaac
     7561 gaagcgcgcg cgggcatggt ttccagcatt accgtgcttg ccatcgggct tttgtcatac
     7621 agcgcggcgg caggcatgat aggcggcggc ggcttgggcg acctcgccat ccgctacggc
     7681 tactaccgct accaaaccga agtcatcgtc ttcatcgtcg ccctcctcgt gctgctggtc
     7741 atcctgattc aaagcaccgg caacgcgttg gcgcggaaac tcgacaaacg ttaaacccga
     7801 atgccgtctg aacgccaaaa accccgccgc tatccgaaaa atgctataaa atccccctgt
     7861 tcgcggcaaa tgccgtctga acgccgaatc cggacggcag gactccctgc ctgtcatttt
     7921 tgtttgaaac cgccacaaca tcaggagaaa atatgaaaac cttcttcaaa accctttccg
     7981 ccgccgcact cgcgctcatc ctcgcagcct gcggcggtca aaaagacagc gcgcccgcag
     8041 cctctgccgc cgccccttct gccgataacg gcgcggcgaa aaaagaaatc gtcttcggca
     8101 cgaccgtcgg cgacttcggc gatatggtca aagaacaaat ccaagccgag ctggagaaaa
     8161 aaggctacac cgtcaaattg gtcgaattta ccgactatgt gcgcccgaat ctggcattgg
     8221 cggagggcga gttggacatc aacgtcttcc aacacaaacc ctatcttgac gatttcaaaa
     8281 aagaacacaa cctggacatc accgaagcct tccaagtgcc gaccgcgcct ttgggactgt
     8341 atccgggcaa actgaaatcg ctggaagaag tcaaagacgg cagcaccgta tccgcgccca
     8401 acgacccgtc caacttcgca cgcgccttgg tgatgctgaa cgaactgggt tggatcaaac
     8461 tcaaagacgg catcaatccg ctgaccgcat ccaaagccga tatcgcggaa aacctgaaaa
     8521 acatcaaaat cgtcgagctt gaagccgcac aactgccgcg cagccgcgcc gacgtggatt
     8581 ttgccgtcgt caacggcaac tacgccataa gcagcggcat gaagctgacc gaagccctgt
     8641 tccaagagcc gagctttgcc tatgtcaact ggtctgccgt caaaaccgcc gacaaagaca
     8701 gccaatggct taaagacgta accgaggcct ataactccga cgcgttcaaa gcctacgcgc
     8761 acaaacgctt cgagggctac aaataccctg ccgcatggaa tgaaggcgca gccaaataag
     8821 gcagtcgtat aaaatgatgc cgtctgaacc gcattccgtg ttcagacggc atttttgtcc
     8881 tttaatccgc cattccctgc cattccgcca aatccggcgt atcgattccg aacagcgaca
     8941 gcgtgtgtgc gatactgtgc gccactatgt cgtccgccgt ctgcggcttg cggtacatcg
     9001 caggaacagg gggaaacacc acgccgccca tttccgttac ccgcttcata ttgtccaaat
     9061 gggcaaggtt cagcggcgtt tcgcgcacca tcagcaccag ccgccgcctt tccttcaaaa
     9121 ccacatccgc cgcacgcgtc aagagattgt cgccgaagcc gtgcgcgaca gaggcaagcg
     9181 tccgcatcga acagggtgcg accagcatcc cgtccgtttt aaacgtaccg ctggcaatgc
     9241 acgccccgat attgccgatc ggatggacga aatcagccaa ggcatatact tcgtctttcg
     9301 tataatccgt ttccgaagcg cgcgccatct ccgcgccttt cgataccaca aggtgcgttt
     9361 cgacatcttg cgcgcgcaaa agttccaaag ccttcacgcc gtattggaaa ccgctcgccc
     9421 cgctgatgcc gattatcaaa cgccgtacca tcatccgcct ttcccataaa accgcctgca
     9481 acggcaaacc ggctattata gtgaaaaaac agaaatccga taaacacgga tacaaattgt
     9541 cggcaacacc caatatccga taaaataccc gatttaacat cctatctgaa taggcacggg
     9601 agggcggtat ggcaaaagta aaaggcggat tggggcgcgg cttggattcg ctgctcgcca
     9661 acggtgcgga caacagcagc ggcgaccggc tgactgcggt tgcggttaaa gatatccggc
     9721 ccggccgcta tcaggcgcgc gtccaaatcg atgacgaagc cttgcaggaa ctggcagatt
     9781 cgattaaggc gcaaggcgtg atacagcccg tcatcgtgcg cgaacacgga ctgtcccgat
     9841 acgaactgat tgcaggcgaa cgccgttggc gcgccgctca gattgccggc ctgtccgaaa
     9901 tccccgccgt tatcaaaacc atcagcgacg aaaccgcatt ggcaatgggt ttgatcgaaa
     9961 acctccagcg cgaaaacctc aaccccatcg aagaagcaca aggcttgaaa cgccttgccg
    10021 acgagttcgg gctgacccac gaaaccatcg cccaagccgt cggcaaaagc cgaagcgcga
    10081 tttccaacag cctgcgcctt ttaagcctgc ccgaatccgt gcaggaaatg ctttaccaac
    10141 gccgcctcga aatggggcac gcccgcgcac tgctgaccct gcccgtcgtc gaacagctcg
    10201 aattggcgca aaaggccgtc aaaaacggct ggtcggtgcg cgaagtcgaa cgccgcagcc
    10261 aggccgccct tcaaaacaaa cgtcccgagc caaaaaaaac tgccgccgcc gacatcggcc
    10321 gcctgaatga tttgctgact gaaaaattgg gtgtcaacgc cgaaatcaag accgccaacc
    10381 ataaaaaagg caaaatcatc ctgcatttcg acacgcccga aaccttcgac catatcttga
    10441 agcagttggg catagattac cggccttaat tttgcgggat atgccgtttg aaatatagag
    10501 aatagctttc cagattttaa gtgggaaata taattctatt gacatttttt ctgcttcgcg
    10561 taagaatcgt tttcctgttt tcatttttaa ttttcgagga aattatgaac acacgcatca
    10621 actttaccgc aactgtgggt aagcgcgtga gccaatgcta tgttaccagt gtaatcagca
    10681 caatcggcgt taccacttcc gatgcaattt gttcgggagg cggaacgaac aaagccaaaa
    10741 gtcaatgcaa tgctttgctt aaagcggcag gccgctgcta atcctttatt cggaaaaggt
    10801 cgtctgaaaa tattttcaga cgacctttta tttattgagc aaactcccca aactgccgac
    10861 aatccgaccg ataaactact acaatttcgc gttgattttt tattatttcc acgtaaagca
    10921 aacaaatcac gtaagaacat taaaagtgta aagaaattgc ggacaaactc cttaatatcc
    10981 tgtaatcata tgtaatatgt gttagaatag cggagttgga ttttggggag tcgctcgtct
    11041 gtatcggcgg cgagtttcac aaagattaac atattgcttg acgtaataag gcttctaaat
    11101 tatattagcc gcgccccaaa cctgaggcgg aaacattccc tttatgaagc agattatcat
    11161 cctacaatcc gccgtattgt ccatatgcgc cgcagttgcc tttgccgtat ggggttttgc
    11221 cggattcctt tcggcggtcg ggggcggttt gtcctacctg cttcccacgt ttgttgcagt
    11281 tttacttcta aaacttttca ggggaaaccc cttcctgcaa agcaggatgt tcgtcttcgg
    11341 agagatttta aaagtagtgc tgtcgctgtt gtccatgctt gccgtatttg caatatggca
    11401 tcaatcgctg gtgttcgtcc cgtttttgat ggggctgctc ggtgtcagcc atctggtttt
    11461 tttagtattg ttgagagtga aagattatgg caggtgaaac cattaccgct gccgactaca
    11521 tcaagcacca cttgcagagc ttgaccagtt tgtcggatgt tactcagggt cagggactga
    11581 aaaacattgc tgatttttcg tttattaacc ttgatgccgt tttttttgcc gtcctgttgg
    11641 gcgtaatcgg cagcttcctg ttgtggcgcg gtgcaaaaaa agcaacggca ggcgttcccg
    11701 gacgtttcca ggccgccgtg gaaatcttgt tcgagtttgt ggacgatatg tgtaagagca
    11761 tcattcacag cgaaaagtct agaaaagccg tcgcgccgtt gggtctgacg ctgtttgttt
    11821 ggattttcct gatgaacgcg atggatatgc tgccggtcga tttgctgccg atggtatggc
    11881 agggcattac cggcaaccat cacgccctgc tgcgcatcgt accgactgcc gatttgaaca
    11941 ccgctttggc actcgctgtc ggcgtgttgc tgatttgtat ctattacaac atcaaaatca
    12001 aaggattggg cggctggttt cacgagttgt tcagtgcgcc gttcggtgca aaactcgcac
    12061 ctgcgaactt tctgttgaac ttggtcgagt ttctttctaa aaccgtatcc cacggtatgc
    12121 ggttgttcgg caatatgtat gcgggcgagc tggtattcct gctgatagcc ttgcttggcg
    12181 gtgcttgggc ggcttccggc agcgttgaag tcatggatcc gattctgttt gtattccaca
    12241 ttattgccgg tttggcatgg gcgattttcc atattttggt gattaccctg caggcattta
    12301 ttttcatggc gttggcgttc gtctatatcg ggcaggcgca cgatgcccac tgattttccc
    12361 aagtagtttg tttttgtagt ttaacctttg tttctttaag gagtttaaaa tgggtttgat
    12421 tgctatcgca tgtggtttga tcgttgcatt gggtgcattg ggtgcatcta tcggtatcgc
    12481 aatggtcggt tctaaatatt tggagtcttc tgctcgccaa cctgaactga ttggtccgct
    12541 gcaaaccaaa ctgttcctga ttgccggtct gattgatgcc gcattcttga tcggtgtcgc
    12601 cattgcacta ctgttcgcct tcgtcaaccc gtttgcaggt gcataatcag gacggattcg
    12661 tccgttcgta caggtgcagc ctgtttgatt aaccccaata cgaaggttaa gtaacgtgaa
    12721 tatcaatgca acattattcg ctcaaatcat cgtctttttc ggtttggtat ggtttaccat
    12781 gaaatttgtg tggccgccga ttgcaaaagc tttggatgag cgtgccgcaa aaatcgccga
    12841 gggcttggct gccgccgagc gtggtaaaag cgatttcgag caggctgaaa aaaaggttgc
    12901 agaacttttg gcagaagggc gtaatcaggt ttccgaaatg gttgccaacg ccgaaaaacg
    12961 tgccgccaaa attgtcgaag aagccaaaga acaggcttct tccgaggcgg cgcgcattgc
    13021 agctcaggca aaggccgatg tggagcagga attgttccgc gcacgcgaat ccctgcgcga
    13081 tcaggttgcc gtgttggctg tcaaaggtgc cgaatctatt ttgcgcagcg aagtcgatgc
    13141 ttccaaacac gcaaaactgc tcgataccct gaaacaggag ttgtaatctt atggcagagt
    13201 tcgcaacgat cgccagacct tatgcaaagg cattgttcgg tctggctcag gaaaaaaacc
    13261 aaattgagtc ttggttgggc ggactggaaa aacttgcggc ggttgttcag gaagggaagg
    13321 tggcttcatt gattgaccgt cctgaaacga atgcttcaga aaaagcagat atcctcatcg
    13381 atttggtcgg tttgaaagac aaggagttaa aaaactttgt tatcgtcttg gccgggcaga
    13441 aacgtttgtc gatattgccg gaagtgtatg ctcaatatca agacttgacc ttatcattca
    13501 accatatcaa atctgccgtc atttacagtg cctatccgtt gaccgacaaa caggtcggcg
    13561 agttggcgca aatgctgaat aagcgtttcg acagcgagct gaaaatctct gtcgaaatcg
    13621 aaccggagct gattggcggc ataaaagttg aagtgggtga tcaggttttg gatttgtctg
    13681 tacaaggcaa actgagtgct ttgtacacga ctatgacgaa ttaggagagt tttcatgcag
    13741 cttaatcctg ctgaaattag cgatctgatt aaagccaaga tcgaaaatct gtctgtaaat
    13801 gccgaagtga gtacccgtgg tacggtgatt tcggtaactg acggtatcgt tcgcatccat
    13861 ggtttgtcag atgcaatgca aggtgagatg ctcgaattcc cgggtaacac tttaggcttg
    13921 gcgatgaacc tggagcgcga ctccgtcggt gccgtagtgt tgggcgagta cgaacatatt
    13981 aaagaaggcg acacggttac ctgtaccggc cgtattttag aagtgccggt cggacgcgaa
    14041 ttggtcggac gcgtcgtcga tgcattgggt cggcctatcg acggcaaagg cccgattaat
    14101 acaactttga ccgcccctgt cgaaaaaatc gcaccgggcg tgattgcacg caaatcggtt
    14161 gatcagccga tgcaaaccgg tctgaaggcg attgactcta tgattcctgt cggtcgcggt
    14221 cagcgtgagt tgattattgg agaccgtcag acaggtaaaa cggccgtggc attggatgcc
    14281 atcgtcaacc aaaaaggtac gggtgttatc tgtatctatg tcgctatcgg tcaaaaagca
    14341 tcttctattg ccaacgtggt ccgcaaattg gaagagcatg gcgcgatgga acacacgatt
    14401 gtggttgctg caactgcatc tgaagcggcg gcattgcaat atatcgcacc ttattcaggt
    14461 tgtacgatgg gtgaattctt ccgcgatcgt ggcgaagatg ccttgattgt ttatgacgat
    14521 ttgtccaaac aggctgtggc ttaccgtcaa atttccctgc ttttgcgccg tccgcccggc
    14581 cgcgaagcct atcccggcga tgtgttctac ctgcactccc gtctgttgga acgtgcggca
    14641 cgtgtcaatg aacacgaagt ggagaaattg accaacggcg aagtaaaagg caaaaccggt
    14701 tctctgaccg cgttgccgat tatcgaaacc caagcgggcg acgtatctgc tttcgttccg
    14761 actaacgtca tttcgattac cgacggtcag attttcttgg aaaccgacct cttcaacgcc
    14821 ggtatccgtc ctgcaatcaa tgccggtatt tccgtatccc gcgtaggcgg tgctgcacaa
    14881 accaaagtga ttaaaaagct gggtggcggt atccgtttgg cgttggcaca atatcgtgaa
    14941 ttggcggcgt tctcgcaatt tgcatccgat ttggatgaag ctacgcgcaa acagttggag
    15001 catggcgaag tcgtaaccga actgatgaaa cagaaacagt tcagtacgtt gaatacggct
    15061 gaaatggctt tgaccctttg ggcaatcaac aacggttcgt attctgatgt tccggttgcc
    15121 aaagctctag cttttgaatc tgaatttttg agctttgtcc gtacccaaca tccggaagtt
    15181 ttggaagccg tcaatgcttc aggtgcaatg tccgacgaga gcgagaaaac gcttgaagca
    15241 gccatgaaat ccttcaaatc ttcttacgcc tatcaggcat aaggcttgaa gtgaaaggag
    15301 tctgaaatgg cagtaggaaa agagattctc accaaaatcc gcagtgttca gaatacccaa
    15361 aagatcacta aagcgatgca aatggtgtca acctctaaaa tgcggaagac tcaggaacgg
    15421 atgcgtttgg cgcgtccgta tgccgaaaaa gtgcgtatgg tgatgagcca tcttgcgcaa
    15481 accaataccg atcatggtat tccgttactg gaatctcatc gggaaatcag acgtgtcggt
    15541 tttattttga ttacgtctga taagggtttg tgtggcggtt tgaacgccaa cgtgctgaaa
    15601 aagtttttgg cacaagttca agagtatcgg aatcaaggta ttgaagagga aatcgtatgc
    15661 cttggcagta aaggtctgat ggcgtgtcag agcattggtc tgaatgtggt tgccagtgcc
    15721 gtaaatttgg gcgatacccc aaaaatggaa atgctgctcg gacctttgac agaacttttc
    15781 caacggtatg agaaacatga aattgacaga atccatctgg tgtattcggg ttttgtcaat
    15841 accatgcgtc aagaaccgag gatggaagta ttgctgccta tcggtgagaa cgtgattggc
    15901 gattcagctc ccaaatcacc gttcagctgg gaataccgct acgaaccgac tgcacttgca
    15961 gtgttggaat atctggttcg ccgctattta gagtctgtgg tttatcaggc gttgagcgac
    16021 aatatggcat ccgaacaggc agcccgcatg gttgccatga aagctgcaac agacaatgca
    16081 ggcaatgcca tcaaagagtt gcgtctggta tataacaaat cgcgtcaagc tgcgattacc
    16141 acggaattgt cagaaattgt agcaggtgcg gcggccgtct gatgccgtct gaatcctgta
    16201 cggaaattag gatacgataa tgagccaagg caaaatcgta caaattatcg gtgcggttgt
    16261 tgacgtggaa tttccacgcg acatgattcc gcgcgtttac gacgctttga aattagacga
    16321 aaacggtctg actttggaag tccaacagct tttgggtgat ggcgtagtcc gtaccatcgc
    16381 tatgggcagc tcggacggtt tgaaacgcgg catgactgtg agcaatactg gttcgcccat
    16441 tactgtgccg gtaggtaaag gtacgttggg acgcattgtc gatgtattgg gaacgcctgt
    16501 tgacgaggca ggtccaattg ataccgacaa gagtcgtgcc atccaccaag ccgctcctaa
    16561 gtttgacgaa ctgtcttcca caaccgaatt gcttgaaacg ggcattaaag tgattgactt
    16621 gctgtgtccg tttgccaaag gcggtaaagt aggtctgttc ggcggtgccg gtgtgggtaa
    16681 aaccgtgaac atgatggaat tgatcaacaa catcgccaaa gcgcacagcg gcttgtccgt
    16741 gttctcaggc gtaggtgagc gtacccgcga aggtaacgac ttctaccacg agatgaaaga
    16801 ttccaacgta ttggataaag tagccatggt gtatggccaa atgaacgaac ctccaggcaa
    16861 ccgtctgcgc gttgctttga ccggtttgac tatggctgaa tacttccgtg acgaaaaaga
    16921 cgaaaacggt aaaggtcgtg acgtattgtt cttcgttgac aacatctacc gttacactct
    16981 ggccggtacc gaagtatccg cactgttggg ccgtatgcct tctgcagtgg gttaccaacc
    17041 gacattggct gaagaaatgg gtcgtttgca agagcgtatt acctctaccc aaaccggttc
    17101 cattacttcc atccaagccg tatatgtacc tgcggatgac ttgactgacc cgtctccggc
    17161 gacaactttc gcccacttgg acgcgaccgt cgtattgagc cgtgatattg cctctttggg
    17221 tatttacccg gcagttgacc cgcttgactc tacttcgcgc caattggatc cgatggtatt
    17281 gggtcaagag cactacgacg tagcgcgcgg tgtacagtct actctgcaaa aatacaaaga
    17341 attgcgcgac atcatcgcca ttctgggtat ggacgaattg tctgacgaag acaaactggc
    17401 tgtaatgcgt gcccgtaaaa tccaacgctt cctgtctcaa ccgttccacg ttgccgaagt
    17461 gtttacaggt tctccaggca aatatgtcgc cctgcgcgat accattgccg gcttcaaagc
    17521 catcttgaac ggcgaatacg atcatctgcc cgaacaggca ttctatatgg tcggcagcat
    17581 cgaagaagcg gttgagaaag cgaaaacctt aaactgagga ggtcggcatg agcatcatgc
    17641 aagttgaggt ggtaagtggc gagcagaaaa tctattcagg cgaggcaaca tttatcgttg
    17701 ttccgactgt acagggcgaa ctcggtattt atccgcgaca cgagccgatt atgagtttgg
    17761 tgcggccggg ggctttgcgt ttgaccgttc cgggcgagga taaagaggtt ttggttgctg
    17821 tttccggcgg tattttggaa gtacagcctg ataaagtaac tgtcttggcg gatgttgccg
    17881 tccgcagtgc ggaaatggat cgggcacgtg cggaagaggc gaaaaaagcc gcagaagcgg
    17941 gcatttccca agctaaagac gataaggctt tggcggaagc acataaagca ttggctgccg
    18001 caattgcaca gctcaaaact ttggactata tccgttcgca caagaaataa acttgtttgg
    18061 tttgaaaaag cacgaccgtt tggccgtgct ttttattttc cggatttaaa ttaaaaaatt
    18121 atccatcccc tggtttcaaa tcaggctgtc ttcgagataa atctattcaa gcggctatgg
    18181 aaacagtaaa ataaaacctt tattttatcg aacaataagc catgcttacc ttccaacaaa
    18241 tcatcttcaa actgcaaacc ttctgggcag acaaaggctg caccgtcatc caacccttcg
    18301 acatggaagt cggtgccggc acatcccatc ccgccacctg cctgcgcgcg ctcggccccg
    18361 agccttggtt tgccgcctac gtccaaccca gccgccgccc caaagacggc cgctacggcg
    18421 acaaccccaa ccgcctgcaa cactattacc aattccaagt cgccctcaaa cccgcccccg
    18481 ccaatatcca agacctctat ctcgactccc tgcgcgaatt gggcatcgat cccaaagtcc
    18541 acgacatccg ctttgtcgaa gacgactggg aaaaccccac cctcggtgcg tggggtttgg
    18601 gctgggaagt ctggctcaac ggcatggaag taacccagtt tacctatttc caacaagtcg
    18661 gcggtatcga ttgcacgccc gtactcggcg aaattaccta cggcatcgaa cgcttggcga
    18721 tgtacctgca aggcgtggaa aacgtctatg acctcgtttg ggcaaaaacg ccggacggca
    18781 ataccgtaag ctacggcgac gtgtaccatc aaaacgaagt cgagcaatct acctacaact
    18841 tcgaatacag cgatgccgac tggctgctgc gccagttcaa cgactacgaa gcgcaagcca
    18901 aacgcctgtt cgccgaagaa aacgccgacc ttgccttacc tgcctacgag ctggtcctca
    18961 aagcggggca tacgttcaac cttttagacg cacgcggcgc gatttccgta accgagcggg
    19021 caacttatat tggacgtatt cgtgcattga gccgcgccgt ggcgcaaaaa tatatcgaaa
    19081 gccgtgagaa actgggcttc ccgttgatta aaaaataacg atacgtcgtc tgaagcaggt
    19141 tttcaggcga cctttattgc gtatccatta atacaggccg tacggcatat gcaggcttta
    19201 taagcatggc ctacggacaa acagaaagtc tcacatgaca acccaaaccc ttttaatcga
    19261 actccttacc gaagaactcc cgccaaaagc cctgaataat ctgggcaacc attttgccgc
    19321 ttccgttgcc gaaggcttgg aaaaagcgca actggttgac ggcgcggccg aatttacggc
    19381 ttatgcctcg ccgcgccgtt tggcggttca agtcaaaaac gtcaaagctg ttcaggccga
    19441 tcagaaaatc gtgaaaaaag gccctgccgt ggcgaatgcc gtaaaagacg gtacgccgac
    19501 caaggctttg gaaggttttg cgcgcggggc gggggcgaaa atcgaagacc tgaccatcat
    19561 ccacgacggc aggcaggacg tgtacgccta cgaatacgtc caaaccggca gaccgttggg
    19621 cggacttttg gaagacatta tcaatcaagc tgttaagaag ctgccgattc cgaaagtaat
    19681 gcgttggggc agcagcacgt ttaccttcgt gcgccctgtt cacgggctga ttgtgctgca
    19741 cggcggcgat gtcgtgaacg tcagcgtttt gggcctgcaa agcggcaatc aaaccttggg
    19801 acaccgcttt ctttccgacg gcgaaatgat cattgaaaac gccgacagct acgccgcaca
    19861 aatgcgcggg caaggcaaag tcgtcgcttc gtttgccggg cgcaaagccg cgattcagac
    19921 ggcattggaa gggcaggcac gccgtctgaa cgcgaccgtt gccgccgatg aagccctgtt
    19981 ggacgaagtg accgcgctgg tcgaatggcc tgttgttttg gaagccggtt ttgaggaaca
    20041 cttcctcgcc gtgcctcaag aatgcttgat tttgacgatg cagcaaaacc aaaaatactt
    20101 cccgctgctc gaccaaaacg gcaagctgat gaaccgcttc ctgctggttt ccaacctgca
    20161 aaccgaagac ccgtcgcaca tcatccgagg caacgaacgc gtattgcgcg cgcgcctgtc
    20221 tgatgccgag ttcttctaca aacaagacca aaaagcgacc ttggaaagcc gcctgcccaa
    20281 gctggcgaac gtggtgtacc acaacaaaat cggttcgcag gccgaacgca tcgaacgcct
    20341 gcaaagcatc gccgcccaca tcgccaaagc attgggcgcg gatgccgcgg cagccgagcg
    20401 cgccgcgcgt cttgccaaag ccgacttggt aactgaaatg gtcggcgagt tccccgaact
    20461 gcaaggcacg atgggcaaat actacgcccg cttggacggc gaaaccgaag aaatcgccga
    20521 agctattgag caacactacc aaccgcgttt tgccggcgac aagctgcccg aaagcaaaat
    20581 tgccgccgcc gtggcacttg ccgacaaact ggaaaccctg gtcggcattt ggggcatcgg
    20641 tttgattcct accggcgaca aagaccccta cgccctgcgc cgcgccgcct tgggtatttt
    20701 gcggatgctg atgcagtacg gtttggacgt gaacgaactg attcagacgg cattcgacag
    20761 cttcccgcaa ggtttgctca acgagaaaac gccgtctgaa accgccgact ttatgcaggc
    20821 gcgccttgcc gtgttgctgc aaaacgatta tccgcaagac atcgttgccg ccgtactcgc
    20881 caaacagccg cgccgtttgg acgatttgac cgccaaactg caagccgtcg ccgtattcaa
    20941 acaactgccc gaagccgccg cgctcgccgc cgccaacaaa cgcgtgcaaa acctgctgaa
    21001 aaaagccgat gccgcgttgg gcgaagtcaa tgaaagcctg ctgcaacagg acgaagaaaa
    21061 agccctgtac gctgccgcgc aaggtttgca gccgaaaatc gccgccgccg tcgccgaagg
    21121 caatttccaa actgctttgt ccgaactggc ttccgtcaag ccgcaggttg atgccttctt
    21181 cgacggcgtg atggtgatgg cggaagatgc cgccgtaaaa caaaaccgcc tgaacctgct
    21241 gaaccgcttg gcagggcaga tgaacgcggt ggccgacatc gcgcttttgg gcgagtaacc
    21301 gttgtacagt ccaaatgccg tctgaagcct tcaggcggca tcaaattatc gggagagtaa
    21361 attgcagcct ttagtcagcg tattgatttg cgcctacaac gtagaaaaat attttgccca
    21421 atcattagcc gccgtcgtga atcagacttg gcgcaacttg gatattttga ttgtcgatga
    21481 cggctcgaca gacggcacac ttgccattgc caaggatttt caaaagcggg acagccgtat
    21541 caaaatcctt gcacaagctc aaaattc
//
LOCUS       DBJGGI010000030        20930 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.030, whole genome shotgun sequence.
ACCESSION   DBJGGI010000030 DBJGGI010000000
VERSION     DBJGGI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20930
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.030"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(21..485)
                     /locus_tag="ACSIGJ_001150"
     CDS             complement(21..485)
                     /locus_tag="ACSIGJ_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330018.1"
                     /translation="MKRFILPVLLSATTALASPIVGTWHCIGTDENIHSDTKVKYLQD
                     GSFRGDAILKIDDDGNILAYRVVGEGKWRFANNALTQSQIKYGEVSRQHSPETLAWLE
                     KSEDARLLESMMYTGLVAQMDKPGKDDVYQLDKSGKLVSEDGTSREACTKVE"
     gene            complement(562..3399)
                     /locus_tag="ACSIGJ_001151"
     CDS             complement(562..3399)
                     /locus_tag="ACSIGJ_001151"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HHQ1330019.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFRPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDGVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESMEEQGSMWHIRYPLADNPTEAVIVATTRP
                     ETLLGDAAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQPGFSLPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKPHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDNEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQAEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKAVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLDKLLMKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGHSFPDMWIIGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPETDGGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGETGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEARQVAAL
                     PESGDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(3504..4763)
                     /locus_tag="ACSIGJ_001152"
     CDS             complement(3504..4763)
                     /locus_tag="ACSIGJ_001152"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HHQ1330020.1"
                     /translation="MKVLVLGAGVAGVSSVWYLAEAGHEVTVIDRTEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKRLFKSHPPLLFRPDGGLYQIEWLWRMLQNCTATRYQ
                     INKERMVRISEYSREMFRRFEAQTDMNFEGRKKGTLQIFRQTEEVEAAKQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIVGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     YFNQTISRIDHNGLRIKAVETETGRFETDAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYETKLPEKRRETLAL
                     VVNDLFPEGGDLSQALSWSGLRPMTPDSTPLIGRTRFENLFLNTGHGTLGWTMSPGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQKL"
     gene            complement(4842..6233)
                     /locus_tag="ACSIGJ_001153"
     CDS             complement(4842..6233)
                     /locus_tag="ACSIGJ_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HHQ1330021.1"
                     /translation="MQVFLDNLKAFFETVSSWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGAGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIVINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPELSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAALYRVGYVSSVMPGTVLSLDLVWLASDTFNGLMALPNLTALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            6439..6579
                     /locus_tag="ACSIGJ_001154"
     CDS             6439..6579
                     /locus_tag="ACSIGJ_001154"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330022.1"
                     /translation="MWRAAEIINAADCHTSEIYSGLNLNRYSVASPCRTSCTVCGFVA
                     LS"
     gene            complement(6696..7472)
                     /gene="lpxA"
                     /locus_tag="ACSIGJ_001155"
     CDS             complement(6696..7472)
                     /gene="lpxA"
                     /locus_tag="ACSIGJ_001155"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HHQ1330023.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVRIGANTEIGPHA
                     VINGHTTIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTVGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFQDFFAQSTRGIIR"
     gene            complement(7515..8195)
                     /locus_tag="ACSIGJ_001156"
     CDS             complement(7515..8195)
                     /locus_tag="ACSIGJ_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HHQ1330024.1"
                     /translation="MERYKNAVRKDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CFRQEFKDRNRLYDSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDAAGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINNFRNRLAHHEPICFKGAIKNTGYARNIYQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            complement(8402..8851)
                     /gene="fabZ"
                     /locus_tag="ACSIGJ_001157"
     CDS             complement(8402..8851)
                     /gene="fabZ"
                     /locus_tag="ACSIGJ_001157"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HHQ1330025.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            complement(8887..9930)
                     /gene="lpxD"
                     /locus_tag="ACSIGJ_001158"
     CDS             complement(8887..9930)
                     /gene="lpxD"
                     /locus_tag="ACSIGJ_001158"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HHQ1330026.1"
                     /translation="MIPATCTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAIIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRHVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTIVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHIAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDSKDTQ"
     gene            complement(9963..10463)
                     /locus_tag="ACSIGJ_001159"
     CDS             complement(9963..10463)
                     /locus_tag="ACSIGJ_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HHQ1330027.1"
                     /translation="MTRLTRAFAATLIGLCCTTGTHADTFQKIGFINTERIYLESKQA
                     RNIQKTLDGEFSARQDELQKLQREGLDLERQLAGGKLKDAKKAQAEEKWRGLVEAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQDVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(10529..12907)
                     /gene="bamA"
                     /locus_tag="ACSIGJ_001160"
     CDS             complement(10529..12907)
                     /gene="bamA"
                     /locus_tag="ACSIGJ_001160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HHQ1330028.1"
                     /translation="MKLKQIASALMMLGISPLAFADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSDRFDRQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTRQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAGTKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSASWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDIYGKAFDPRKASTSVKQYKT
                     TTAGGGVRMGIPVTEYDRVNFGLAAEHLTVNTYNKAPKRYADFIKQYGKTDGADGSFK
                     GLLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGRTYTAAENGNNKSVYSENAH
                     KSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(12964..14304)
                     /gene="rseP"
                     /locus_tag="ACSIGJ_001161"
     CDS             complement(12964..14304)
                     /gene="rseP"
                     /locus_tag="ACSIGJ_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HHQ1330029.1"
                     /translation="MQTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARTGFQSGDKIQSVNGVSVQDWSSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLTYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTVEWIRGKPLGERVQNIGLRFGLALMMLMMAAAFFNDVTRLIG"
     gene            complement(14339..15574)
                     /gene="ispC"
                     /locus_tag="ACSIGJ_001162"
     CDS             complement(14339..15574)
                     /gene="ispC"
                     /locus_tag="ACSIGJ_001162"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HHQ1330030.1"
                     /translation="MPSENASDGIRYKVILIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFRPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAAGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAIFQVLPRDYTDRLNEHGIDSIILTASGGPFLTTDLSTFDSITPE
                     QAVKHPNWRMGRKISVDSATMANKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGKLDFGALSALTFQKPDFGRFPCLKF
                     AYETINAGGAAPCVLNAANETAVAAFLDGQIKFTDIAKTVAHCLAQDFSNGMGDIEGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(15579..16376)
                     /locus_tag="ACSIGJ_001163"
     CDS             complement(15579..16376)
                     /locus_tag="ACSIGJ_001163"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HHQ1330031.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALTALWEYA
                     RMAGLCKTETNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLLMPFWFALVSLRPHPDDALPLLAVMGLVWVADVCAYFSGKALGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLPGHGGVFDRTDSLIAVISVYAAIMSVLN"
     gene            complement(16379..17125)
                     /locus_tag="ACSIGJ_001164"
     CDS             complement(16379..17125)
                     /locus_tag="ACSIGJ_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HHQ1330032.1"
                     /translation="MKSSTQTILEHTAIPRHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDET
                     ALDAAVASFQKRERRFGRTSEQLPIGQQRN"
     gene            complement(17181..17738)
                     /gene="frr"
                     /locus_tag="ACSIGJ_001165"
     CDS             complement(17181..17738)
                     /gene="frr"
                     /locus_tag="ACSIGJ_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HHQ1330033.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYIAEADKVLAAKEEDLMAV"
     gene            complement(17880..18872)
                     /locus_tag="ACSIGJ_001166"
     CDS             complement(17880..18872)
                     /locus_tag="ACSIGJ_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HHQ1330034.1"
                     /translation="MKIISLFSGCGGLDLGFEKAGFEIPAANEYDKTIWATFKANHPK
                     THLIEGDIRKIKEEDFPEEIDGIIGGPPCQSWSEAGALRGIDDARGQLFFDYIRILKS
                     KQPKFFLAENVSGMLANRHNGAVQNLLKMFDRCGYDVTLTMANAKDYGVAQERKRVFY
                     IGFRKDLEIKFSFPKGSTVEDKDKITLKDVIWDLQDTAVPSAPQNKTNPDAVNNNEYF
                     TGSFSPIFMSRNRVKAWDEQGFTVQASGRQCQLHPQAPKMEKHGANDYRFAAGKETLY
                     RRMTVREVARIQGFPDNFKFIYQNVNDAYKMIGNAVPVNLAYEIAAAIKKTLER"
     gene            complement(18877..19281)
                     /locus_tag="ACSIGJ_001167"
     CDS             complement(18877..19281)
                     /locus_tag="ACSIGJ_001167"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021068.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoPII family restriction endonuclease"
                     /protein_id="HHQ1330035.1"
                     /translation="MVYGIDYCADAECYLKIKNQIKEGIGNIGGIQFAETKELGRVNR
                     IDPLNITYLRVRGMWGIENPWFVFNYIYQRNMEKSFNFMAIINEDKWNSFNNTDKLLA
                     IQDSKLAISDIKIKNPNNPARLRNAKLITYHL"
     gene            complement(19310..19714)
                     /locus_tag="ACSIGJ_001168"
     CDS             complement(19310..19714)
                     /locus_tag="ACSIGJ_001168"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021068.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoPII family restriction endonuclease"
                     /protein_id="HHQ1330036.1"
                     /translation="MNIIDAIINLANNPVVGVNSHSQSNNRANQAGDALEEYVKDLFS
                     GSFNLNETQRIARHAKVFSYLGNNSNPPDAMLRNGDAIEVKKIESKDSALALNSSHPK
                     SKLSVDDSMLTKACKDAEKWEEKDIIYNCRRR"
     gene            complement(19823..>20930)
                     /locus_tag="ACSIGJ_001169"
     CDS             complement(19823..>20930)
                     /locus_tag="ACSIGJ_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HHQ1330037.1"
                     /translation="RLSGRWLNSYERYRHRRLIHAVRLGGAVLFATALARLLHLQHGE
                     WIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGNLLFYLTI
                     GTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWLDSGLMRAMNVLIGAAIAIAA
                     AKLLPLKSTLMWRFMLADNLADCSKMIAEISNGRRMTRERLEQNMVKMRQINARMVKS
                     RSHLAATSGESRISPSMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSEIRLLDRH
                     FTLLQTDLQQTAALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLSTNMRQEI
                     SALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
BASE COUNT         4246 a         5303 c         5995 g         5386 t
ORIGIN      
        1 gtggaaaata cagttgcttt tcactccact ttcgtacatg cctcacggga agttccgtct
       61 tcggacacta atttgcctga tttatccagt tgatacacat catcttttcc gggtttgtcc
      121 atctgcgcga ccaatccggt gtacatcatg ctttccaaga gtcgtgcatc ctccgacttt
      181 tctaaccacg ccagcgtttc ggggctgtgc tgtcgtgaaa cttcaccgta cttgatttgg
      241 ctttgagtta aggcattgtt cgcaaaacgc catttacctt caccgacaac acggtatgcc
      301 aagatattgc cgtcatcatc gattttgagt atagcatcgc cacggaagct gccgtcctga
      361 agatatttga cttttgtatc actgtgaatg ttttcatcag tgccgatgca atgccatgta
      421 ccgacaatcg gagaagccaa ggcggttgtt gcggataaaa gcaccggcag aataaagcgt
      481 ttcatctatt tcctttttta aaggaggccg tctgaaaacc ccggtttcag gttttcagac
      541 ggccttgttt attcaatcaa atcagtcttt caacttcgcc aactgatttt gcacttttgc
      601 cattttgtct tccaattccg ccaaatcggc tttgtctttt tccaccagat gcgccggggc
      661 tttttcggtg tagccgggtt tggagagttt ggcattgagt ttgtccaagg ctttttgcag
      721 cttctcggct tctttgctca aacgggcggt ttcggcggct ttgtcgattt cgactttcag
      781 catcaggcgc gcgccgttgc agacggcgac gggcgcgtct ccgctttcgg gcagggcggc
      841 gacttgccgt gcttcggtca ggcgggtcat catcggcagg tatttgaggt agtccgccaa
      901 gtcgtccgcg ctttcgacaa acagcggggc tttcacgttg ggctggatgc ccgtttcgcc
      961 gcgcaggttg cggactgcgc cgatcaaatc ctgcaacacg gtcatttgcc cgaatgccgt
     1021 ctgaacaatc tcgccgccgt cggtttcggg gaaacgggcg agcatgatgc tgtcggcggt
     1081 tttggcatcg cacatggggg cgacggtttg ccacagttct tcggtgatga acgggataat
     1141 cgggtgcagc aggcgcaggg cggcttcgag tacgcgcaat aaggtatggc gtgtggcgcg
     1201 ctggcggctg gcgcagccgg tttggagctg cactttggcg agttccagat accagtcgca
     1261 ataatcgttc catacgaagc tgtacagggt ttccgccgcc aaatcaaagc ggtaggtttc
     1321 gtaggcttgc gtaacctgct cgatggtctg attcagacga cctatgatcc acatatcggg
     1381 gaaggaatgg ccgcgcggtt cggcagcggt tgcgccgtag ccgcagtctt ggttttcggt
     1441 gttcatcaag acgaagttgg tggcgttcca gattttgttg cagaagttgc ggtagccttc
     1501 ggcgcgtttg aagtcgaagt taaccgaacg gcccaagctg gcgtagctcg ccatggtgaa
     1561 gcgcaatgcg tccgcgccca tgctggggat accttcgggg aagagttttt tcgtggcttc
     1621 ttccactttc ggcgcggttt cgggtttgcg caggccggtc gtacgtttca tcagcagctt
     1681 gtccaagccg ataccatcga tcaaatccac agggtcgatg acgttgcctt cggatttgga
     1741 catttttttg ccttcgtggt cgcgcacgat gccgtggatg tacacggctt taaacggtac
     1801 tttgccggtg aagtgggtgg tcatcataat catgcgcgcc acccagaaga agatgatttc
     1861 atatccggta accaagacgt tggacggcag gaaggctttg agctcgtcgg tttcagacgg
     1921 ccagccgagc gtggagaacg gcacgagcgc ggaggagaac catgtgtcca atacgtcttc
     1981 ttcgcgggtc aaacctgttt tgccggcttg tttttcggct tcagcctgat tgcgggcgac
     2041 gtaaacatta cctgcttcgt cgtaccacgc ggggatttga tgcccccacc acagttggcg
     2101 cgaaatacac cagtcttgga tgttgttcat ccattggttg taggtattga cccagttttc
     2161 agggataaag cgcactgcgc cgctgtcgac ggcttttttg gctttgtcgg caaggctcaa
     2221 gcctttgaat tcgttgtcag gctcgccgcc gttcggtgtg gcggacatgg cgacaaacca
     2281 ttggctggtc agcatcggct caatcactga acctgtgcgg tcgcctttcg gcgtcatcag
     2341 cgtgtgcggt ttgatttcga ccaagaagcc ttgctcctgc aaatcggcaa ccatttgttt
     2401 gcgcgcggca aagcggtcca agcctgcgta tttttcaggc agggaaaagc ctggttgcgc
     2461 ttcgcctttg aagttgaaca cttcggcgtt tgccagcact ttggcttcta agtcgaacac
     2521 gttaatcagg cgcgtgtcgt ggcgtttgcc gacttcgtaa tcgttgaaat cgtgcgcagg
     2581 cgtgattttc acgcaaccgg taccgaagtc tttttcaacg tattcgtcgg cgataaccgg
     2641 aatggtgcga ccggtcagcg gcaggattaa ttctttgccg attaagtggg tgtaacgttc
     2701 atcttctgga ttgacggcaa cggccgcgtc gcccagcaac gtttcaggac gggtggtcgc
     2761 cacgataacg gcttcggtgg gattgtcggc cagcggatag cggatgtgcc acatagagcc
     2821 ttgttcttcc atgctttcca cttccaaatc cgataccgcc gtgccaagca cgggatccca
     2881 gttcaccaag cgtttgccgc ggtaaatcaa gccctgttca tacaggcgca cgaacacttc
     2941 ggtcacggtt tcggcgcgta cgccgtccat cgtgaaatac tcgcgcgtcc agtcggcgga
     3001 gcagcccacg cggcgcattt gctgggtaat cgtgccgccg gaaacttctt tccattccca
     3061 cactttttcc aagaattttt cgcggcccaa gtcatgacgg gacacgtttt gcgcggcaag
     3121 ctgacgctcg accacgattt gcgtggcgat gcccgcgtgg tcggtaccgg gaatccaggc
     3181 ggtgttgcag cctttcatgc ggtagtagcg ggtcaggccg tccatgatgg tttggttgaa
     3241 ggcatgaccc atgtgcagcg tgccggttac gttgggcggc ggcagttgga tggaaaaaga
     3301 cggtttggtc aaatccatat cgggccggaa atagccttgg ctttcccagt tttgatagtg
     3361 tttggattcg atttcggcgg gggagtattt gtctaacata tgaagccctg agaaataaga
     3421 tgatttgatg gtttgaatta tagcgcaaat gccgtctgaa acgctttcgg ggtttcagac
     3481 ggccttaaat ttaaatattg cctttacagt ttttgatagc gcgacaggct caaatcgtcg
     3541 ctgcggattt cggtgtcttt gccgctcacg atatcggcgg tcaattttgc cgaacccggc
     3601 gacatggtcc agcccaaagt accgtgcccg gtattcagaa acaggttttc aaagcgggtg
     3661 cgcccgatta acggcgtact gtcgggcgtc atcggcctga ggccgctcca ggacaatgct
     3721 tggctcaaat cgccgccttc cgggaacaag tcgttgacga ccaaagccaa ggtttcgcgg
     3781 cgtttttcgg gcagtttggt ttcgtagccg gacaattccg ccattccgcc gacgcggatt
     3841 ctgttgtcaa aacgcgtgat ggcgactttg tagctttcat ctaaaacagt ggacaccggt
     3901 gcgccgtcgg aatttgtgac cggcagggtc aaggaatagc ctttgacggg ataaatgggc
     3961 agattgagat ccaactgcgc caacacagtc ctgctgaagc agccgagcgc gcagacaacg
     4021 gcatctgttt caaaccgccc tgtttcggtt tcaacggctt tgatgcgcag cccgttgtgg
     4081 tcgatgcggc tgatggtttg gttgaagtag aaccgtacgc ccttctcttg acacaatttg
     4141 tacaggtttt cggtaaagag gcggcagtcg ccggtcgcat ccgcaggcag gtgcagaccg
     4201 ccgacaattt tggcggtaac gcgtgccagc gcaggctcga attctgcgca ttcttcgggc
     4261 ttcagacggc ggtacggcac gccgtagcgt tccaaaacgg caatgtcttg ttttgccgct
     4321 tcgacttctt cggtttggcg gaaaatctgc aacgtccctt ttttgcgtcc ctcaaaattc
     4381 atgtcggttt gcgcttcaaa acggcggaac atttcacggc tgtattcgga aatcctgacc
     4441 atgcgctctt tattgatttg atagcgcgtt gccgtgcagt tttgcagcat ccgccacagc
     4501 cattcgattt gatacaggcc gccgtcaggg cggaacagta aaggcggatg gcttttaaac
     4561 agccgtttca gtgctttggt cggaataccg ggtgcagccc aaggcgtggt atagccgtaa
     4621 gaaagctggc ctgcattggc aaaactggtt tccatcgcca caccctcggt gcggtcgatg
     4681 accgttactt catgtccggc ctctgccaga taccacacgg aggatacgcc ggcaacaccc
     4741 gcacctaaaa caagcacttt catgtttccc cctccggctt tttcaaaaca ggcttaatat
     4801 gccgtgccgt ctgaatattc ggattcagac ggtctcggat attaatgcgg caattcgccg
     4861 tttgtgattt tttgtttgaa gtcgcgtgtt tcattgacga tgactttcgc catcaacaaa
     4921 agcgcagtca ggttgggcaa tgccatcaag ccgttgaatg tatccgaagc cagccatacc
     4981 aaatcaaggc tcaacacggt acccggcatg acggaagaaa cataaccgac gcggtacaaa
     5041 gcggcaaact tctcgccgaa gacgtaaacc gcgcatttct cgccgtaata acaccagccg
     5101 agaatggttg agtaggcaaa gaaaatcagg ccgatggtca caatccagcc gccgatgccg
     5161 ggcagcattt tttggaaagt gacggttgtc agcgccgcgc cgctcaattc aggtttcaca
     5221 aactcgccgc ctgcgccgag cagacccatg accaacacga taccggtaat cgagcagaca
     5281 acaatggtgt ccaaaaaagt accggtcatg gaaaccaaag cctgacgaac ggggtggtcg
     5341 gttttcgcgg ctgcggcggc aataggcgca gaacccatac ccgcctcatt ggagaacacg
     5401 ccgcgcgcca cgccgtagcg gatgaccgta ccgatagcgc cgcctgctac ggcctgcgcg
     5461 ctgaacgcat cggagaaaat cagcttgacg gcaggcatca gtgcatcgga attaatgacg
     5521 ataatggaaa gaccgcccaa cacataaaac accgccatag caggcacgat gaaagaagcg
     5581 gctttggcga tgcctttaat accacctaaa acgacaacgg cagtcagaac ggtcaacgta
     5641 atgccggtat aggcaggttc gataccgaag ctggtttgca cagcctgtgc aaccgagttg
     5701 gactgcaccg agctgccgat accgaaggaa gcgaatgtgc cgaacagcgc aaatgcaacg
     5761 gccatccatt tccagttttt gcccaagcct ttttcgatgt aatacatcgg gccgccggac
     5821 atttcgcctt tggaattgtt gacgcggtat ttcaccgcca acacgccttc gccgtatttg
     5881 gtggccatgc cgaaaatagc ggtcatccac atccaaaata ccgcgcccgg gccgccggtt
     5941 accactgcag tcgccacgcc ggcgatgtta cccgtaccga tggtggcgga cagcgcggtc
     6001 atcaacgccg caaaatggga aatatcgcct tcgtgacctg cgccgccttc atacttcttt
     6061 gacggcacaa acgcctgttt cagcgcatag cccaacatag tgaactgcaa gcctttcagc
     6121 ataacggtca gcacaatgcc ggtgccgacc agcagcatca acataacaga tccccaaacc
     6181 cagctgctga cggtttcgaa aaaggctttg agattgtcta aaaatacttg catggctttc
     6241 tcctttgtct gttttatttt taaaacacca cttttgtagt gtccggtaat ttcagcacag
     6301 aatattcaat aagacaatat gttcttttga aaaatacttt tggttttttc accgaagaca
     6361 ggccggttca agctgcggaa attgtttgca attatttaaa agcagaggcg gaggtcacaa
     6421 tgaaatgtcc gaatggggat gtggcgggcg gcagaaatca tcaatgctgc cgactgccat
     6481 acttctgaaa tctatagtgg tttaaattta aaccggtaca gcgttgcctc gccttgccgt
     6541 acttcttgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttaaatttaa accactatac
     6601 aaaatgatgc atcgatcaaa caacataccg ctttaaaaaa aaccgatgcc gtctgaaacg
     6661 ctttcggggt ttcagacggc attgaaaggg tgcggtcagc ggatgatgcc gcgcgtcgat
     6721 tgtgcgaaga agtcttgaaa tacggcaagc tcggcttggg tttcggcgcg gcggagaatg
     6781 tccgccttgg cttcttcaaa cggaatgccg cgatggtaga gggttttgta tacgtctttg
     6841 acggcggaaa tctgttctgc ggtaaaaccg ttgcggcgca taccttcgct gtttaggccg
     6901 gccggttcgg cgcggtagcc cgatgccata aagtagggcg gcacgtcttt gtgtacgcct
     6961 gcggcgaagg cggtcatggc gtagtcgccg atgcggcaga attggaagac cagcgtgtag
     7021 ccgcccaaaa cgacgtagtc gccgacggta acgtgtccgg caagcgaggc gttgttggcg
     7081 aaaatggtgt ggttgccgac cacgcagtcg tgcgcgaggt ggcagtacgc cataatccag
     7141 ttgtcgtcgc cgatacgggt ttcgccgatg ccggttaccg tgcctaaatt aaaggtggtg
     7201 aattcgcgga tggtgttgcc gttgccgata atcagcttgg tcggctcgtc gcggtatttt
     7261 ttgtcctgcg gaatttcgcc gaggctggca aattgaaaaa tgcggttgtt ttcgccgatg
     7321 gtggtgtggc cgttgatgac ggcgtgcgga ccgatttctg tattcgcacc gattcggacg
     7381 ttggggccga taacggtgta cgcgccgact ttgacgccgg agtcgagttc ggctttgggg
     7441 tcgatgacgg cggtcgggtg gatgagggtc atgttttgaa tactccgctg cttgttaaga
     7501 caaataatag aaaatcacag tttgtcgatt tcatcgcaga ccgcaatcac ttgatcgcta
     7561 aaatggctaa atactgaagc agtatctaca tccatgtaat taagcagttc aaaaatagat
     7621 tggtaaatat ttctagcgta gccggtattt tttattgcac ctttaaaaca aatcggttcg
     7681 tgatgtgcca aacgattacg gaaattgtta atattggaaa gttctcgaaa aatccatttt
     7741 tggttatatt gcacacttgg tgtagatttg ggcttttttg gaaaaacttt catcaatact
     7801 tttcctgcag catcaaattg agcatctttc cctcccgcaa acagataccg ccaaaaaccg
     7861 aatcctagtc ctgcaaccaa ttggttgtgg tcaaactttc ctccgctatt attttgtatt
     7921 ttagataatg caaccttgat taattcagca gattctttcg taccatttct taaaaagcaa
     7981 ccttgatatt tcaatgcagg attcgtttgt ggttgtatgc tatcatataa ccggtttctg
     8041 tctttaaact cctgccgaaa gcaaatatca attttgtttc ttaaaacgat ctcaaatata
     8101 gaaacaacat gaaaaagttc tcttgataaa gataaattca ataaatacaa ttcagctgct
     8161 ttgtctttcc taacggcatt tttataccgt tccattctga aatcagaaaa aatactacag
     8221 cattccttat aattcacgct aacccttgat tctttatttt taaattgacg tttcaaacta
     8281 tatttgatat agttagataa aactaatccc caccgtgtcc ctgggtttga ataaacctaa
     8341 accggtgggt tttcttttta ttaggtcgtc tgaaactttc agacgacctt tttccgaacg
     8401 ctcaaaccac gcgtttggcg cacataatca cggcttcgac ggcgacttgt ccgtccactt
     8461 tggcaacggc gttgaatttg ccgatgccgc gccggctggt caggagttcg acttcaaaga
     8521 cgagttggtc gccggggatg acttggcgtt tgaaacgggc ttcgtctatg ccggcgaaga
     8581 agaaaaattc gttttccttg cgcccgcctt cgctcaaaat cgccaacgtg ccgcacgcct
     8641 gcgccatcgc ttcgatgatg agtacgccgg gcataacggg caggtcgggg aaatggcctt
     8701 ggaattgggg ttcgtttatg gttacgtttt tgatggcggt cagggttttc atcggctcga
     8761 aggcggtaat gcggtcgagc tggagaaacg ggtagcggtg ggggatgagt ttttggatgt
     8821 ctttggcttc gatggggagt tgtacgtcca tgtctgtcgt attccttgaa taaagtcggt
     8881 ttggatttat tgcgtgtctt tgctgtctga aagctgctgt tccagcgttt tgaggcgttt
     8941 gttcatttcg cttaagcggt ggatgtaaac ggcgttgcgc gcccattctt tatgggtgga
     9001 catcgggaag atgccggcga tgtgtttgcc gctttcggta atgctgtggg taacggacgt
     9061 gccgccgccg atggtggttt tgtcggcgat ttcgatgtgt ccgaccgtgc cgactccgcc
     9121 gccgatgatg cagtagctgc ctatggttac actgccggag atgcccgttt tggcggcgat
     9181 gacggtgtgc gaaccgattt tgcagttgtg tccgatttgg acttggttgt cgattttggt
     9241 gccgttgccg acaatggtgt cgctcatcgc gccccggtcg atgttggtgt tcgagccgat
     9301 ttctacgtcg tcgcccagcg ttaccgcgcc ggtttgcggg attttgaacc acgaatcgcc
     9361 ggcgaaggcg agtccgaaac cgtccgcgcc gatgaccgcg ccgctgtgga tttcgacgtg
     9421 tctgccgagt gtgcagccgt aataaacgac ggcgttggga tgcaggacga cttcgtcgcc
     9481 cagtttgcaa tcgtgttgga cgacggcgtt tgccaagatg cggcagcctt cgccgagcac
     9541 ggtgttcgcg ccgatgtagg cgttcgcgcc gatttcacag cttgcgggaa cggtcgcgcc
     9601 cggttcgacg acggcggtcg gatggatgcc gccgcgcgct ttgacgacgg gtgaaaacag
     9661 gcgcgcgact ttggcgaaat agagataggg atcgtcggcg acaatcaggt tgcgcccttc
     9721 aaatccgtct gctgctttgg cggaaacgat gatcgcgccc gcgctgctgt cgtggacttc
     9781 ggctttgtat ttcggattgg cgaggaagct gatgtgttcc gcctgcgcgt ctgcgagcgg
     9841 gcgcacggcg gtaacggaaa tgtcctcgcc gcgccattcg ccgccgagcc gcgcggtgat
     9901 ttgggacagg gtgcaggtgg ccggaatcat ggttttcctg tttggtatgc cgtctgaaag
     9961 ggttagcggg cgttcatttc tttaatgacg ctgtcggtaa cgtcgtattg ggtgttgacg
    10021 taaatcacgt cctgcaaaat gacatcgtaa ccttcctgtt tggcgatttt gacgatgacg
    10081 cggttggcgt tttgctggag ggaggcaaac tcttcgttgc ggcggaggtt gtagtcttct
    10141 tcaaactgcg cctgtttttt gcggaacgct tcgaccagcc cgcgccattt ttcttcggct
    10201 tgcgcctttt ttgcgtcctt aagtttgccg ccggcgagct gcctttccaa atccaagcct
    10261 tcgcgttgca gtttttgcaa ttcgtcctga cgggcggaaa attcgccgtc cagcgttttt
    10321 tggatgttgc gcgcctgctt ggattcgagg tagatgcgct cggtgttgat aaagccgatt
    10381 ttttggaagg tgtcggcgtg cgtgcctgtg gtgcagcata aaccgatcag ggtggcggca
    10441 aacgcgcggg tcaaacgggt catggtaaaa ctccttcgga tgttgccgcg aaatgccgtc
    10501 tgaagggctt cagacggcat ttgcgggatt agaacgtcgt gccgagctgg aattggaagc
    10561 gttggatttc gtcttccggt tttttcttca gcgggtaggc gtagctgaat ttcatcgggc
    10621 ccaaaggcga gagccaggta accgcgccgc cggcggaata gcgcaattcg ttggtaaagg
    10681 tggatttatg cgcgttttcc gagtaaaccg atttgttgtt accgttttcg gcggcggtat
    10741 aggttctgcc gtcccacacg ctgcctgcgt cggcaaacag gctcaggcgg acggtgcgtg
    10801 cgtctttcgc accgggcatc gggaagagca gctcggcgga gacgttggct tttttgttgc
    10861 cgccgtagct gattttttcg ccgtattcgt catacacttt cgggccgagc gtgccgcttt
    10921 cgtagccgcg caccgaaccc aggccgccgc cgtagaagtt ttcaaagaag gggatttctt
    10981 tggttctgcc gtagccgccc gcaatgccga cttcgccgcc gagcatcagc gtgaaggttt
    11041 tgcttaaggg gaagaaccag gtttggttgt gggtggcgga gtagtattgc agtttgctgc
    11101 cgggcagggc gatttcggca tttacgccgg tcaggtagcc gcgcgtcggc cataaggcgc
    11161 tgtcggtctt gttgcgcccc cagccgacag tgcctttgta cagcaggcct ttgaagctgc
    11221 cgtctgcgcc gtcggttttg ccgtattgtt tgataaagtc ggcatagcgt ttgggtgctt
    11281 tgttgtaggt gttgacggtc aggtgttccg ccgccagccc gaaattgacg cggtcgtatt
    11341 cggtaacggg gatacccatc cttacgccgc cgccggcggt ggtggtttta tattgtttga
    11401 cgctggtcga tgctttgcgc gggtcgaagg cttttccgta aatatcgtag cccaggctga
    11461 ccccgtctgc cgtgaagtac gggtcggtaa acgacagcga gccgttgagc gtggttttgc
    11521 ttcgcgaggc gcgcagggcg gccgacttgc ccgtaccgaa caggttgtcc tgcgatacgc
    11581 cggcggacat gaccaagccg gtatcctgaa cccagctcgc gctcaagtcg agcgagccgg
    11641 tggagcgttc ggtcaggctc atgttcaaat cgactttgtc gggcgtgccg gcaagcggga
    11701 cggcatcaaa ctgtacgttg tcgaagtagc ccaaaagctc gacgcgctct ttggaacgtt
    11761 gcagcttgga ggtgtcgtaa ggcgcggatt ccatttggcg caattcgcgg cggacgactt
    11821 cgtcgcgggt tttgttgttg ccggtgatgt ggatttcgtt gacgtagatt tttctgcccg
    11881 gttcgatgtg caggacgaaa tcgacggttt tggttccggc gttcggcagc ggctgtacgc
    11941 tgatttcgct gtatgcgtag cctgccgagc ccatgcggtt ctgaatctca cccaaaacgg
    12001 cggtcatctg ctggcgttcg taccatttgc cgggcttcat ggtcagcagt ttttccagtt
    12061 cggccttggg gacttcgttg gtgtcgcctt caatcgacac tttgccccag cggaaacgtc
    12121 cgccttcgtg gacggtgatt ttgatggtct gcctggtttt gtcttcgttg gtttggatgt
    12181 cggtatcgag gatacggaaa tcgaagtagc cgttgttctg gtagaagtcg gttacttttt
    12241 ccatgtcttg ggcgaatttc tggcggtcga accggtcgct tcgtgtcagc catgtccaaa
    12301 tgccgccttc ggtcagcgac atctgccgca tcagtttgcg gtcggaatag acttggttgc
    12361 cttcaaattc gatgtcggtg attttggcgg atttgccctc gtcaatcgtg atgtcgatgt
    12421 cgacgcggtt gcgggcgagt ttggttactt tgggcgtgat ttggatattg agtttgccgc
    12481 gcccgaggta ttcttctttc aggccggcga ctgcctggtt gagtgtcgcc tgattaaagt
    12541 attgcgactg cgccagcccg aacgattcga ggtttttctt gatggcgtcg ttttgcagca
    12601 ttttggcgcc ggtgatgttg agcgagccga tggtggggcg ttcgataacg gtcagcagaa
    12661 gctgcccgtc cgcagtttcg actcgtacgt cgtcaaagaa accggtggcg tacaggcttt
    12721 tgatgatggc actgccgtgt gtgtcgttgt aggtgtcgcc gactttgacg ggcaggtagt
    12781 tgaatacggt gctcggctcg gtacgctgca agccttcgac acggatgtct tggatggtga
    12841 agtcggcaaa tgccaaaggc gatatgccca acatcatcag tgcggaggca atctgtttca
    12901 gtttcatcgt aagttccttg tggtgcggaa tgcggtttca gactgcattc cgaaacgtaa
    12961 aatctaaccg atcagccggg taacgtcgtt gaagaaggcg gccgccatca tcagcatcat
    13021 cagggcgagc ccgaagcgca aaccgatgtt ttggacacgt tcgcccaaag gtttgccgcg
    13081 tatccattcg acagtataaa acacgaggtg cccgccgtcc aaaacgggga cgggcagcag
    13141 gttcagcacg ccgaggctga tgctgaccaa cgctaaaaat tccaaataac tttgcaagcc
    13201 gagttcggcg gactgtccgg caatgtcggc aatggtcagc ggcccggaaa tatggctgac
    13261 agaggcgttg ccgctgatta gtttgccgaa aaatttgagg gttgtccacg agtgggaaac
    13321 ggttttttcc cagcccatgc cgaatgcgcg gacaacagac ggacggtagc tgcggcggat
    13381 ttgcgcgtcc cacgccctgt ccggctgcgg acggaggccg acgcgcccga tcagggtgtg
    13441 gtcgggctgt tcgacagtat cggggcggat gtcggcggta tgggtttgtc cggcgcgttc
    13501 gtaggtcagg gtgatttttt tgcccgggct ttggcgggtc aggtttgccc attcctgcca
    13561 tgaggcgatg ggtttgccgt cggcggcagt cagcctgtcg cccggtttca ggcctgcttt
    13621 ttcggcgggg ctgccttttt ccacgccgcc ggcaacggtt gtgattttaa agggcatcag
    13681 tccgatgtag ccttggtttt ttgcgatttt accggcttcc ggcgtgcctg cggcatcgat
    13741 ggtgcggacg gtttgcgcgc ccgatgccgt ctgaacgccg acggcgactt tgccggcttc
    13801 gaggttgagg acgatttcgg tttgcgcgct gctccagtct tggacggaaa cgccgttgac
    13861 ggattgtatt ttgtcgccgc tttggaagcc ggtgcgggcg gcaatggtgt cgggttcgac
    13921 tgtgccgaca tagggccgca gttcggttac gccgaaggaa aagctcagtc cgtacagcaa
    13981 aaccgccagt gcgaggttgg tcagcggacc ggcggcgacg atggcgatgc gcttggcggg
    14041 gtgttgtttg tcaaaagcgt agggtaaatc ggcttctgat acttcgcctt cgcgcgtatc
    14101 gaccattttg acgtagccgc ccaacggaat cggggcgagg caccattcgg tgtcgccgcg
    14161 ctttcgggtg aaaaacggtt tgccgaagcc gacggaaaaa cgcacaacct tgacgccgca
    14221 caacctggcg acgatgtagt gtccgaattc gtgcaggctg accaaaatca ggatggcgaa
    14281 gataaaagct agaagggttt gcaaatggtt ttcctttgat aacggtgttc ggacggtatc
    14341 agcgcagtgt gccgataaat gcccgcgctt gtgcgcgtgt ccgggcatct tgcgccaaca
    14401 gtccttctat atcgcccatg ccgtttgaaa agtcttgtgc aagacagtgg gcgacggttt
    14461 tggcaatgtc ggtaaactta atctgtccgt ccaaaaaggc ggcgacggcg gtttcgttgg
    14521 cggcgttcaa tacgcagggc gcggctccgc ctgcgtttat ggtttcatag gcgaacttca
    14581 ggcaggggaa gcggccgaag tcgggctttt ggaaggtcag cgcggacaat gcgccgaaat
    14641 cgagtttgcc gacacccgaa tcgatgcgct cgggcaagcc caaacaatag gcgatgggcg
    14701 ttcgcatatc gggattgccc agttgcgcca gcacggagcc gtcgcggtag cgcaccatac
    14761 tgtgtatcac ggattgggga tggatgacga cttcgagttt gtcgggcgga cagttgaaca
    14821 gccaatgcgc ttcaatcagt tccaagccct tgtttgccat agtggctgaa tcgacggaga
    14881 ttttgcgccc catacgccaa ttggggtgtt tgaccgcctg ctcgggcgta atgctgtcga
    14941 acgtgcttaa atcggttgtt aaaaacgggc cgccggaagc ggtcaggata atcgaatcga
    15001 tgccgtgttc gttcagacgg tctgtgtaat cgcgcggcaa aacttggaaa atggcgttgt
    15061 gttcgctgtc gacgggcaac actgccgcgc cgtttgcgcg ggcggtttcc ataaacaacg
    15121 cgccggaaac caccagcgtt tctttgtttg ccagataaat ggttttgcct ttttgcgccg
    15181 ctgcgagcgc ggaaggcagc cccgccgccc cgacgatggc gcacatgacg ccgctgactt
    15241 cgtcggcaga cgcaacgtca accaatgcct gcgcgccgtg taaaacctga gtcgccgtgc
    15301 cgtcgcgttt caacagggct tcaagccggg cggcgtgttc ggcatcggca acgacggcat
    15361 attcggggcg gaacgtttga cattgagccg ccagtttctc gacctgctta tgccctgcca
    15421 gcgcgaatac gcggaatttt tcgggatggc gggagacaac gtccagcgtg ctttcgccta
    15481 tgctgccggt actgcctaat atggtcagga cttgtggtgt cataatgagg ataactttat
    15541 accggatgcc gtctgaagcg ttttcagacg gcatagaatc aatttaaaac cgacattatc
    15601 gccgcataga cgctgataac ggcaatcagg ctgtcggtac ggtcgaacac gccgccgtgt
    15661 ccgggcagca ggttgctgct gtctttgatg cctgccgcgc gcttgagcca gctttccaac
    15721 aggtcgccgc atacgctgac aacggtcaac accaaaccga ttaacacggt atcgaaccag
    15781 cctgtatcga atgccagcca gccggcactt cgtacggcag tcatgtacac tgccacgcaa
    15841 accgcaccgc cgattgcgcc ttcccagctc ttgccggggc tgattgccgg tgcgattttg
    15901 tgtttgccga gcgccttgcc gctgaaatac gcgcaaacgt cggcaaccca gaccaaaccc
    15961 atcacggcga gcagcggcag ggcatcatcg ggatgcgggc gcagggatac gagcgcgaac
    16021 caaaacggca tgagcaaaag ccagccgacg gcataaacct gccaaccgcc gttgagcctc
    16081 catttgaatc tcaaccacaa aggcataacg gcgagccaaa atgccaaaac aacataccaa
    16141 accaaattag gcagcatcca gccgcccgca taggcaacta cgccgaaaac caaggttgcg
    16201 gcgaggtaat ggttggtttc ggttttgcac aaaccggcca tacgggcata ctcccacaag
    16261 gcggtcaggg caatcagccc gcaaaatgca gcccacagcc attgcggcgc gtaaaacagc
    16321 atgcccagca tcagcggcag cagccacata gcggttatta cccgttgttt cagcatattc
    16381 agttcctttg ctgtccgata ggcagttgct cggaagtgcg tccgaaccgc cgttcgcgtt
    16441 tttggaacga agcgacggcg gcatctaagg cggtttcgtc gaaatcgggc cacaggatgt
    16501 cggtgaaata cagttctgca tacgccatct gccagagcag gaaattgctg atgcgcgttt
    16561 ctccgcccgt gcggatgaac aaatccggtt ccggcgcgtc gcccagcatc aagtgtttgg
    16621 ccagcgtgtc ttccgtaatc tcggatacgc cttcggcaat cagtttgttt gccgcctgca
    16681 aaatatccca gcggccgccg taatccgcag caatgctcag ggtcagaccg gtattatttg
    16741 ccgtcaaggc ttccgcctct tcgatacctt gcagaatctg ccgattaaag cgttcgcggc
    16801 tgcccaatat tttcaggcgc atattgtttt cgtgcaggcg gcgtacctgt ttttgcaaag
    16861 cctgtaaaaa cagccccatc aggaacgaaa cttcgtcttc ggggcgacgc cagttttcgg
    16921 ttgaaaaggc aaacacggtc agatattgca cgcccagttt ggcgcaatgc ttcaccatat
    16981 tttccaacgc gtccaagccg cgtttgtgtc ccattatacg cgggagaaaa cgttttttcg
    17041 cccaacggcc gttgccgtcc ataattacgg cgatgtgcct cgggatggcg gtgtgttcca
    17101 aaatggtctg cgtgctgctc ttcatatctg cctttcgcgg ttcggcgttc aaatgccgtc
    17161 tgaacgccgc gccgtgagat ttaaaccgcc atcaggtctt cttctttggc agccaaaacc
    17221 ttatcggctt cggcaatgta cttgtcggtc agtttttgaa ccgcctcttc gccgcgacgt
    17281 gcctcgtctt cggaaatttc tttgtctttg aggagttttt tgatgtggtc gttggcatcg
    17341 cggcgcacgt tgcggataga gacgcgtcct tcttccgcct cgccgcgcac gactttaatc
    17401 aggtctttgc ggcgttcctc ggtcagcatg ggcatcggca cgcggatcaa atcgccgaca
    17461 gctgccgggt tcagtcccaa gtttgaatcg cggatggctt tctcgacttt ggccgccata
    17521 ttgccctcaa acggtttcac gccgatggtg cgcgcgtcca aaagggttac gttggcaacc
    17581 tggctgacgg ggaccatgct gccccagtat tcgacttcca cttggtcgag caggccggta
    17641 tgcgcgcggc cggtacgcac tttcgccaga ttttctttca gtacttcgac cgaacgctgc
    17701 atcttgcctt cggctgtttt ttgaatatcg ttgatcatat tgttctttcg gtaagataag
    17761 gtaggcgggc agccgttcga acgcgctcca agccgttcag acggcataaa gaccgttaac
    17821 tgcgaatagt accgttattc gggcatgacg acaaggcaga tgcctgtagg aagggtgttt
    17881 cacctttcta gggttttttt aattgccgct gcaatttcgt aggcaaggtt gacggggacg
    17941 gcgttgccaa tcattttgta tgcgtcgttg acattttgat agatgaattt gaagttgtcg
    18001 ggaaagcctt ggattcttgc aacttcgcgt accgtcatcc tccgatatag tgtttctttg
    18061 ccggcagcaa aacggtagtc gtttgccccg tgcttttcca tctttggggc ttgcgggtgc
    18121 agttggcact gcctgcctga agcttggacg gtaaaaccct gttcatccca cgctttaact
    18181 cggttccggc tcataaaaat aggggaaaaa ctgccggtaa aatattcgtt gttgttgact
    18241 gcgtcggggt tggtcttgtt ttgcggggcg gaaggtacgg ctgtgtcctg caaatcccaa
    18301 ataacgtctt tcaatgtaat cttgtctttg tcttcgaccg tcgaaccttt tggaaaagaa
    18361 aattttattt ccaagtcttt acggaaaccg atgtagaaga cccttttgcg ttcctgtgct
    18421 acaccgtagt ctttggcgtt ggccatagtc aaggttacgt cgtatccgca tctgtcaaac
    18481 attttcagca ggttttgtac ggctccgttg tggcggtttg ccagcattcc gctgacgttt
    18541 tccgctaaaa agaattttgg ctgtttgctt ttcaaaatac ggatgtagtc gaaaaacaac
    18601 tgtccgcgcg catcgtcgat gccgcgcaaa gctcccgctt cagaccaaga ctggcagggt
    18661 gggccgccga taatcccgtc gatttcttca gggaaatctt cttctttaat cttgcgtata
    18721 tcgccttcta tcaaatgggt ctttggatgg tttgccttga aggttgccca aatggtttta
    18781 tcgtattcgt tggcggcggg gatttcaaat cccgcttttt cgaaacccaa atccaaaccg
    18841 ccgcaaccgc taaacaaact aatgattttc ataggattat aagtggtagg tgatgagttt
    18901 tgcatttctt aatcgcgccg ggttgttggg atttttaatt ttaatgtcgg aaatggccag
    18961 tttggaatcc tgtatggcaa gcagcttgtc tgtattgtta aaactgttcc atttgtcttc
    19021 gttgataatg gccatgaaat tgaaactttt ttccatattc cgttggtaaa tatagttgaa
    19081 gacaaaccat ggattttcga ttccccacat accccgtact ctcagataag ttatgttgag
    19141 cggatcaatg cggttgaccc tgcccagctc tttggtttct gcaaactgta tgccgccgat
    19201 gttgccgatt ccttccttaa tttgattttt tatcttcaaa tagcattcgg catcggcgca
    19261 gtagtcgatt ccgtaaacca ttgccaagtg tttgaggttt ttctttttgt caacgacgcc
    19321 gacaattata gataatgtct ttctcttccc atttttccgc atctttgcag gcttttgtaa
    19381 gcatgctgtc atcaacagag agctttgatt ttgggtggct gctattcagc gccagtgcgg
    19441 aatctttact ttcaattttc ttaacttcaa tggcatcccc gtttcgcaac atggcatcag
    19501 gagggttgga gttgttgccc aaatatgaga atacttttgc gtgtcgggct atgcgttggg
    19561 tttcatttag gttgaaactg ccggaaaaca agtcttttac atattcttcc aaagcatcac
    19621 ctgcctgatt ggcacgattg ttgctttgag aatgggagtt gaccccgaca acagggttgt
    19681 tagccaaatt gatgatggca tcgataatgt tcatggtttt tccttggggt tttgcggcag
    19741 attatagcaa aaccgtcagg gtgtttatcg gaccgatgcc gtctgaagcg gcaggcgttt
    19801 cagacggcat cgtgtccggc cgtcagccgt gttcccgtgt ttcaagcagg ctttggcgca
    19861 ggtgttggcg ttcgtgggca tccagccatt tgcggcgggt gcgttgcagc aggatgacga
    19921 gggcggaaat ttcctgacgc atattggtgc tgagccagag gaagccctgc cattggtagt
    19981 ggaggtgttc ggcgagggct tccagttcgg ggttgatggc ggtgtcgatg cggatgcggc
    20041 gggcgtgtct gccgttgatg agggcggcgg tttgttgcag gtcggtttgg agcagtgtga
    20101 agtggcggtc gagcagccgg atttcgctgc cgttgagttt gggagattgc agcttggcgg
    20161 cggtggtcag gagcagctcg gtggtgttga cgattttgcg gtgggcgtgc tgcatggctt
    20221 ccatcatgga ggggctgatg cggctttcgc ccgatgtggc ggcgaggtgg ctgcggcttt
    20281 tgaccatgcg tgcgttgatt tggcgcattt tgaccatatt ctgctccaaa cgttcgcgcg
    20341 tcatacgcct gccgttgctg atttcggcaa tcattttgct gcagtcggcc aggttgtcgg
    20401 caagcatgaa acgccacatc agtgtggatt tcagcggcag cagtttggcg gcggcaatgg
    20461 cgatggcggc gccgatgagg acgttcatcg cgcgcatcag gccgctgtcg agccattcgc
    20521 tgccgttgtc gccgatgagc atgcacatcg tcagccccgc cagcataggg acgtagccgt
    20581 ttttgccgac cgccgcccag ccggccagtg cgcttgccgt gccgatggtc aggtagaaga
    20641 ggaggttgcc gtggaaataa tgctggttca gccataaaac gcccaaaccc gcgcccagcc
    20701 cgatgaccgt accgagcata cgttccaccg ccttggagta aatcgcgcct tggaactgga
    20761 gcatgccgag gacgacgaag acggtcatcc ctatccattc gccgtgttgg aggtggagta
    20821 gccgggcgag tgcggtggcg aacaggacgg ctccgccgag ccgcacggca tgtatgaggc
    20881 ggcggtggcg gtagcgttcg taggagttga gccagcggcc ggaaaggcgt
//
LOCUS       DBJGGI010000031        20415 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.031, whole genome shotgun sequence.
ACCESSION   DBJGGI010000031 DBJGGI010000000
VERSION     DBJGGI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20415
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.031"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(<1..108)
                     /locus_tag="ACSIGJ_001170"
     CDS             complement(<1..108)
                     /locus_tag="ACSIGJ_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HHQ1330038.1"
                     /translation="MRNTVGLDISKLTFDATAIVGNAEYSAKFDNDSKGL"
     gene            116..762
                     /locus_tag="ACSIGJ_001171"
                     /pseudo
     CDS             116..762
                     /locus_tag="ACSIGJ_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
     gene            complement(834..2579)
                     /gene="recD"
                     /locus_tag="ACSIGJ_001172"
     CDS             complement(834..2579)
                     /gene="recD"
                     /locus_tag="ACSIGJ_001172"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HHQ1330039.1"
                     /translation="MELQTDEFAQAAARAAIRFLERYAGSGNEVLANCTERLFQALQN
                     GHSFIRLSGDEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGAGSEGQRDAAALALLQFFMVITGGPGTGKTTTVAKLLA
                     LICGENENLPHIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLSPPKMQAAFDHIRPLPFDVLIVDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCSYVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSDAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGKKTFCQAVNTVKVR
                     QTALGSMLERVFSQE"
     gene            complement(2646..3341)
                     /gene="lolD"
                     /locus_tag="ACSIGJ_001173"
     CDS             complement(2646..3341)
                     /gene="lolD"
                     /locus_tag="ACSIGJ_001173"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HHQ1330040.1"
                     /translation="MSDLILKCEGVGKRYRDGGLDVPVLYGLDLEIRTGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRALNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSRKEAVETAMAMLDKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPRQGANA"
     gene            complement(3334..4581)
                     /locus_tag="ACSIGJ_001174"
     CDS             complement(3334..4581)
                     /locus_tag="ACSIGJ_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HHQ1330041.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFAENRKGILAAAPYVSNQAL
                     LANAGEIRGVQIRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGGKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDTVWVRDWTYSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPAGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLWGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
     gene            4807..5085
                     /locus_tag="ACSIGJ_001175"
     CDS             4807..5085
                     /locus_tag="ACSIGJ_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330042.1"
                     /translation="MNIRKISALCAVAVFTVSTAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPVSLNGGSGSITVSGSSAAQCVFTVGSGGTLQIQGLNCK"
     gene            complement(5141..5758)
                     /gene="recR"
                     /locus_tag="ACSIGJ_001176"
     CDS             complement(5141..5758)
                     /gene="recR"
                     /locus_tag="ACSIGJ_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HHQ1330043.1"
                     /translation="MNSKKQDAFQRLIGALKVLPNVGPKSAQRMAYHLLQQKRKEAEE
                     LVDALQTALRQVRHCARCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNIEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLDGGEIEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(5825..7363)
                     /locus_tag="ACSIGJ_001177"
     CDS             complement(5825..7363)
                     /locus_tag="ACSIGJ_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HHQ1330044.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISEHSINNAMQNEQADGGSPWRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQMIV
                     DDPNFHDANGKFSHALLSQYLSQRHMSEDQFVEEIRDQFALQNLVSLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKASEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPAHEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKNLLFEEAKDAVRQAYIRTEAAKLAENKAK
                     EVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLTGLP
                     APVIVEVQAVTPPEDIAAQLPPAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(7412..7786)
                     /locus_tag="ACSIGJ_001178"
     CDS             complement(7412..7786)
                     /locus_tag="ACSIGJ_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HHQ1330045.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFGFRDFKKQMPSEAEICS
                     WLEQVPLATLFNKRGTSWRKLDAETQQKALSSTAEAVKLMSEMPSLIKRPVLECGSRV
                     YVGFSEETYDGIFNRQAPCRQG"
     gene            7936..9564
                     /locus_tag="ACSIGJ_001179"
     CDS             7936..9564
                     /locus_tag="ACSIGJ_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HHQ1330046.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFRNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGREGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALDKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            9643..10875
                     /locus_tag="ACSIGJ_001180"
     CDS             9643..10875
                     /locus_tag="ACSIGJ_001180"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HHQ1330047.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPKTHEEYALARTERKTAKGYAGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRAARFAARYGFEIAEETIKLMRQM
                     VENGEADALVAERVWQELAKGLMEKNPRKMIEMLRECGALQVLLPEVDALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPPDILPRHHGHDINGVE
                     PVREVNQRLRAPRQCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHFIAEQI
                     DRARLAQIAPLQKAFRGA"
     gene            11294..11602
                     /locus_tag="ACSIGJ_001181"
     CDS             11294..11602
                     /locus_tag="ACSIGJ_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330048.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGETRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            11684..12715
                     /gene="ruvB"
                     /locus_tag="ACSIGJ_001182"
     CDS             11684..12715
                     /gene="ruvB"
                     /locus_tag="ACSIGJ_001182"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HHQ1330049.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQH
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMGEEGA
                     MEVAKRSRGTPRIANRLLRRVRDFADVKNNGVIDAAVADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(12960..13643)
                     /gene="rpe"
                     /locus_tag="ACSIGJ_001183"
     CDS             complement(12960..13643)
                     /gene="rpe"
                     /locus_tag="ACSIGJ_001183"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HHQ1330050.1"
                     /translation="MTAYRIAPSILSADFARLGEEVANVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYVLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRQV
                     RAMLDRYEGKSGRRIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIAAM
                     RAELEKAAG"
     gene            13789..14223
                     /locus_tag="ACSIGJ_001184"
     CDS             13789..14223
                     /locus_tag="ACSIGJ_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HHQ1330051.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYHSSMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(14236..15099)
                     /locus_tag="ACSIGJ_001185"
     CDS             complement(14236..15099)
                     /locus_tag="ACSIGJ_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHQ1330052.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHVFPLSRLAGVGRQTELLVRNTEQFIAGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVMVYATSNRRHLMPEYLDENAGTTGVRGEIHQKEAVEEKVSLSDRF
                     GLWLSFYPFDQNDYLAAVRSWLEDFDVPYDEAARTAALQWAQMRGNRSGRSAWQFACD
                     WAGRLPEQRAP"
     gene            complement(15107..15724)
                     /locus_tag="ACSIGJ_001186"
     CDS             complement(15107..15724)
                     /locus_tag="ACSIGJ_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HHQ1330053.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAAENLQHGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFALPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKAGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(15875..16489)
                     /locus_tag="ACSIGJ_001187"
     CDS             complement(15875..16489)
                     /locus_tag="ACSIGJ_001187"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HHQ1330054.1"
                     /translation="MKTSNFPKSPPALKTFALILAGGLADRMGGEDKGLALLEGRPLI
                     DRVIGKIRLQVSHIVISANRNLEEYARRSPHVFPDARQWQHFGPLSALCTAANDLQLA
                     AADWLLIVPCDMPYLPDDLVARFESVSKRTPLCNAFYVETPVTMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQTDLQEG"
     gene            16696..18465
                     /locus_tag="ACSIGJ_001188"
     CDS             16696..18465
                     /locus_tag="ACSIGJ_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HHQ1330055.1"
                     /translation="MILPTRFSDGIPLSLRLKLLTGLWVGLAALSVVLTLLLSFRLEN
                     AASVIEEAGNLKMQAYRLAYMAGEGSPRAQIDNQIAEFEKSLKRISQSDAIHPLIPSD
                     NPLAYDLIQSMLIIDWQANILPPLQAYRRPTQIELYRFAGNIELFLQALENAGEKNTW
                     WLRRFQWVIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGQRHFDIPVPEDGTPE
                     FKQVGRCFNQMALRLKTLYDDLEGQVAEQTHNLEKQNRNLTLLYRTTRDLHQSYTPRQ
                     AAEEFLNHILPAVGAQSGNICLENGSDTDISVHTAEHGKKPPLEKYHDETFPIEYQNE
                     KLGMLSLGFSDGTSLTGDDRTLLQTLIRQLGVSLAGAKQEEEKRLLAVLQERSLIAQG
                     LHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFRT
                     KISNKEFPEAVADLFARFTQQTGITVETVWENGSFLPTQDEQLQMIFILQESLSNIRK
                     HARATHVKFTLSEYGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIRAVL
                     EIRSQAQQGTTVSLTGAPKESLP"
     gene            18462..19118
                     /locus_tag="ACSIGJ_001189"
     CDS             18462..19118
                     /locus_tag="ACSIGJ_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HHQ1330056.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQRTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVPE
                     "
     gene            complement(19313..20338)
                     /locus_tag="ACSIGJ_001190"
                     /pseudo
     CDS             complement(19313..20338)
                     /locus_tag="ACSIGJ_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_304669545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(20345..>20415)
                     /locus_tag="ACSIGJ_001191"
     CDS             complement(20345..>20415)
                     /locus_tag="ACSIGJ_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HHQ1330057.1"
                     /translation="EFRLNFGTPSRQLKILRDRCGI"
BASE COUNT         4717 a         5890 c         5102 g         4706 t
ORIGIN      
        1 taaacctttt gaatcgttgt caaactttgc cgaatattcg gcattgccga caatggccgt
       61 tgcgtcaaat gtcagcttgg atatgtccaa tcctacagtg ttacgcaata tataaggggt
      121 tgccgttccg cagttgggcg gcagcataca cgttcctttc ggcgtattct cgccgttgcg
      181 cggcgaatat ctcgacctgc ttgcccatgc gccgtcaacg ggttttcaga cggcattcga
      241 tatcgggacg ggctcgggcg tacttgccgc cattttggcg aaacagggca ttccttccgt
      301 catcggcacg gataccaatc cgagggcggt tgcctgcgcc cgtgccaata ttgcccgttt
      361 gggttttgaa aaacaggttg aaatccgcga aaccgattta tttcccgaag ggtttgccga
      421 cctgattgtc tgcaatccgc cctggcttcc cgccaagccg acttccgccg tcgaatccgc
      481 gttatacgac cccgaatctg cgatgctggc tgcgtttttg cgtgatgcgc caaacatctg
      541 aatcccgacg gagaaatccg tcttatcatt tccgacctcg ccgtgcattt ggggctgcgg
      601 tctgccgact ttttggaaaa ggcatttatc cgggcgggtt tgcgtgttga ggatgttttg
      661 aaaaccaaac cggttcacaa aaaagctgcc gattcgaacg atccgctggc ttttgcccga
      721 aacagggaaa ctactttcct gtgccgtttg aaaaaggcat aaggggcggt ggcgtgcttc
      781 cgacggtagg catcggaacg cccgcgccgc cggcacggca tcattcgggc ggattattct
      841 tgtgaaaata cccgctcaag catactgccc aacgccgtct gacggacttt gacggtgttt
      901 accgcctgac aaaatgtctt tttgccgccg aaaaatacga atttttccct ggcgcgggta
      961 atggcggtat ataacagctc cttactcaat ccggacaatg catcgtcccc ttcgtccgaa
     1021 ggtgcgtcgg aaggcggcag cagccagact tcccgatatt ccgaaccttg gcttttgtgg
     1081 acggtcatgg cgaatgcggg ttcaaattcg ggcaggcagc ttaccgctac ctttttaaat
     1141 ccgtccgcat cggcaaaata ggcggcaagg ctgccctgcc gtccgacatc ttccataatc
     1201 agtccgatgt cgccgttgaa cagctcaagc gcgtagtcgt tctgtctgat cataatcggc
     1261 tctccggcga aataggccaa atgttccggt atgttcattt tgcggcgtac atagctgcaa
     1321 taggcttcgt tgaagtcttc cgcatcctgc cgccaagctg ccagaaccac gatatccgag
     1381 atgcctgcgt atgcggcttc gatattgccg tcttttaccg cctgccaata ggctttgtgc
     1441 gcccggtaca acctttcgac ccgagcattc ggactgcatt ccgaatgttc cagttcgtcc
     1501 ggaaaccggt caaacaatgc ccacgcccct tcatcgcccg atacggcggc acgggcaagg
     1561 cagccgatgc cgctgttgtc gccgaagcgg tggctgaacg acagatgggc ggtgttttgc
     1621 gccaacacgg gcggatttgc gctgacgctg aaaccgtgtt ccggaaggaa accggccagc
     1681 ctttggtgcg tttctccgtc caaaacggtt ttttgcgaca aaaccgacag caccgccccg
     1741 attccgacgg acgggagctg gttttcatcg cccagcagaa tcacgcgcgc gccggttttg
     1801 accgctttca aaagttgcag catcaatgcg gtatccaaca tggaggcttc atcgacaatc
     1861 agcacgtcga acggcagcgg acggatatgg tcgaacgccg cctgcatttt gggcgggctc
     1921 agcttaagca gtcggtggac ggtttgccct tccagtttga gcaaatggcg gcggacggcc
     1981 tccggcgcgt caaaaccgtt gattgcacgg tgcagcgcgc gcgccatatg tgccgccgct
     2041 ttgcccgtcg gtgcggcaag cgcgatatgg ggaagatttt cgttttcacc gcaaatcagt
     2101 gccagcagtt tggcaaccgt tgtcgttttg cccgttcccg gccctccggt aatcaccata
     2161 aaaaactgca acagtgccaa ggcggccgca tcgcgctgtc cttcgcttcc cgcgccttga
     2221 aaccattttg cgaggttttg ccgcgcgcct gccgcgtcgg gggcggacgt gccggctgcc
     2281 gccaagcgtt ttatttcggc agccaaatcg tattccaact gccacatcct gcccaaaaac
     2341 agccttctgc cttccaaaat caaaggcgcg gcggatgttc cgaccacggg tgcgagtgcc
     2401 gacagcgcgt cagcctcgtc cccgctcaaa cggataaacg aatgaccgtt ttgcaatgcc
     2461 tgaaacaggc gttcggtaca gtttgcaagc acttcgttgc ccgaacccgc atagcgttcc
     2521 aaaaaacgga ttgccgccct tgccgccgct tgggcaaatt catctgtctg cagttccatc
     2581 gtattccttg tcgaaatgcc gtctgaaggc acggggcttc agacggtgcg tgttgtttcg
     2641 gctgtttagg cgtttgcgcc ctgtctgggg tgcaggctgc cgtctttcat gaccatcacg
     2701 cgctcgaagc ggccggcgag ttcgtcgtcg tgcgttacga ccaccagccc cgttcccaat
     2761 tccgttttca gttccagcat catatccaaa acattccttg cgtttgcccg gtcgaggtta
     2821 ccggtcggtt cgtcggcaag caggcatttg ggttgcgtaa ccaaggcgcg cgcaatggcg
     2881 gcacgctggc gttcgccgcc ggacaattcg cccgcacggt gcgtcgaacg gtgtttcagt
     2941 ccgaccttgt cgagcatcgc cattgccgtt tctaccgcct ctttacggct ttttttgccg
     3001 atcagaagcg gcatcatcac gttttccagt gccgaaaatt caggcagaag atgatggaac
     3061 tggtacacga aaccgagatg gcggttgcgc aaatcgccca aacgccgctg gtttaaggca
     3121 cgcaaatcct cgcccatcag cagcaccctg ccttcagacg gcatatccag cccgcccaaa
     3181 atatgcagca gcgtcgattt gccgctgccc gaagagccga tgatgccggt gctttccccc
     3241 gtgcggattt ccaaatccaa gccgtacagc accggaacgt ccaaaccgcc gtcacggtag
     3301 cgtttgccca cgccttcgca tttcaaaatc aaatcactca taacgcaaag cctccgccgg
     3361 ttgggttttt gacgcgcgcc agctcgggta cagtgtcgcc acgaaagaca gccccaagga
     3421 aatgcaggca atcagggcaa cgtcgcccat atcgacatca ctgggcaggt agtcgataaa
     3481 ataaacctgc gaattgatga ggtggacacc gagcaggttt tcaaaaaacg ccacgaccct
     3541 gccgacgttc caaccccaaa gcacgccgca gaccacaccc gccagcgtgc cgaaaaagcc
     3601 tgaaaacgcg ccctgcacca taaaaatctt catcacgccg gcaggggaaa gacccaaagt
     3661 ccgcaaaatc gcaatgtccg cctgcttttc cgtaaccgcc atcaccaagg aagagacaag
     3721 gttgaacgcc gccacggcga taatcagcgt caggataatg aacatcatcc gtttttccag
     3781 ttcgaccgct tcaaaatagc tgcggttgct gtacgtccaa tcgcgcaccc aaaccgtgtc
     3841 cctttgcgcc tccggaatca gcgttgccgt caaggcgggg gcgttttgcg gatcggcaag
     3901 cttcagccgc agccccgcaa cttccttatc caagcggtac agtacgcgcg cgtcttggat
     3961 atgcgtcatc gccaatgagt tgtccacttc gtaaacaccc gtcttgacca ggccgaccac
     4021 ggtaaactgc ttcaggcgcg gcacgactcc ggcaggggta acgttgccct ccggcgtaat
     4081 taccgtaacc ttgccgccga cttccgcccc caaagcctcc gccaaaccga caccgaggat
     4141 aatgtcaaac tcgcccggaa tcaggtcttc aaacttgcct gccggcattt tgtcgccgta
     4201 ttccaccact ttgcgctctt cagacggcaa aatgccgcgt atctgaacgc ccctgatttc
     4261 gcccgcgttt gccagcaatg cctgattgga aacatagggt gcggcagcca aaatgccttt
     4321 gcggttttcg gcaaaccgaa gcaggttgcg ccaatccgta tccgtattgt cgatatagcc
     4381 gatttcggcg tgcggcgcga cattcaggag ctgcccgcgt atttctttct gaaagccgtt
     4441 cataaccgac aagacgacaa tcagcgcggt tacgcccaag gcgattccgg caatcgaaac
     4501 catcgtgata aacgacataa agccgttgcg ctttttcgcc ctgagatacc tcaagcctat
     4561 ccaagcctct agagaaaaca taacgctacc ttaaaaatgt ctgcaaacgt gccgccccgg
     4621 acggcggttt gggggaggcg gcaaaagttt attgtaccgt aaaaccccgg cagcgtccga
     4681 acggcaggct gtgtaaataa agacgcggta tgtggcaaat tcggaaaaac aggacggaaa
     4741 acttgcacaa cgcgccgaac taccctatcc ttctcttgaa acaaaacctt ttctttaagg
     4801 aaaacaatga atatcaggaa aatctccgct ttgtgtgctg ttgccgtttt tactgtttcg
     4861 acagcctatg ccaaagaaat caaaatcgat gccaacaaca cgccttattc cgaagccgac
     4921 gcgcaaaagc tggcggcaac ggcagtcggt atgggcgtta aggaacctgt cagcctgaac
     4981 ggcggcagcg gcagcattac cgtttccggc agcagcgcgg cgcagtgcgt ctttacggtc
     5041 ggcagcgggg gcactttgca gattcaaggg ctcaactgca agtaaaccgc ccgaaaaaat
     5101 gccgtctgaa ggcttcagac ggcattttgc attggcggcg ttacgccccg ccttctttaa
     5161 tcagacgccg ttcgtacacc gcctgcgcca gcgttcccgc atcgacatat tccaattcgc
     5221 cgcccaatgg gatgccctgc gacagcctgc tgaccttgta aggcaggttt ttgaaaaact
     5281 cggacaggac atacgctgtg gcattgcctt ctgcggtaaa ggcggtcgcg ataatgattt
     5341 cttcgatttc cccgccgtcc agccgttgcg ccagcctgtc caatgcgatg gcggatacgt
     5401 ccattcccaa tgccgtattg atttgcccca tcaggacgaa atacagcccg tcgtggcagt
     5461 ttgccgcttc tatattcgac acgtcggcag gcatatgcac caccatcagc cgccgcccgt
     5521 cgcgtgtttc atcggcacaa atatcgcaca atccgccttc gcaaaatgta ttgcaccttg
     5581 cgcaatggcg aacctgcctc aatgccgtct gtaaggcatc caccagctct tcagcctctt
     5641 tgcgcttttg ctgcaacaga tggtacgcca tccgctgtgc cgatttcggc ccgacgttgg
     5701 gcaatacttt cagcgcaccg atcaggcgtt ggaatgcatc ctgttttttg gaattcatca
     5761 tactccgcca tatgggaaaa cggtcggaat attccgaccg ttatgttgtc aacaaaagtg
     5821 tcaattactg accatcgccg ttgtcaaccg attgtgctcc tttagtctgt ttgatttttc
     5881 cgttgaaata gcggatcagc aggtcgaaag tattggcaga ctgctgttgc gccaaagcct
     5941 gtttcgcagg aggaagctgt gcggcaatat cctccggagg cgtgactgcc tgcacctcga
     6001 caatcacggg tgccggcaga ccggtcagtc tgacataggc gggtttgccg tttgccggtt
     6061 ttgctttcag cagttccgca taagcctcgg gcggcatgga ctgccttgcc tgctgcgcgc
     6121 ccaaaacgga cacttccgac catttcacgt caactgcctt gccgccgttc agttgggtaa
     6181 gcacttcttt tgccttgttt tcggcaagtt tggcggcttc ggtacggata taggcctgac
     6241 gcaccgcatc tttggcttct tcaaacagta ggtttttttc ttcgcggact tctttggcgc
     6301 ggacgaccca cgcggtttcg ctgttgatgg tcagcacttc ggaattgtgt tttttcttca
     6361 atacgtcgtc gctgaatacg gcattgatta ggttttcggg catgccggac atttgtgcgt
     6421 cctgcctgct cagccaagtt tcttgggttt ccactttcaa accgctgttt ttggcggctt
     6481 cggcaagcga ggagggatga ttgaacgcat cgtcgcccag cttttctttt gccttgttga
     6541 agtcggcaac cgcctttttc attttcaatt cgttttcgac ggcggctttt tcctgctcga
     6601 aagaaggttt ggcttcatgt gccggcaaac gcgccacgcg ctcttcaaac gcatttttca
     6661 cttccgtttc actgacggtc tgcttgtctg caaaatcctt cagattcaag gcgacatatt
     6721 ccaatttgac cgcctgcggc agcagatagt cttttttgtt cgcattataa aatttctgca
     6781 aatcggcttc agacgctttg acttgggcga tgaactcgtc ggggttgaaa gtgtgcgaac
     6841 ggatggtgcg gttgacctgc gtcagcctga tcagctgttc cgcctgcgcg tcgccgacca
     6901 atacgccgtt ttggacgagg cttaccaaat tctgcaaggc aaactgatcg cggatttctt
     6961 cgacaaactg gtcttcagac atatggcgtt gcgacaggta ttgactcaaa agcgcgtgac
     7021 tgaatttgcc gtttgcgtcg tggaaattgg gatcgtccac aatcatctgc ttgatttgtt
     7081 cggaagaaac cgaaatgccc atcagcttcg caccctgttt caggtaggcg cgttgcagca
     7141 gggattggaa caccgcgtcg cgccaagggc tgccgccgtc cgcctgctcg ttctgcatgg
     7201 cgttgttgat tgagtgctcg ctgatttttt cgtcgcccac ttggacgatg tagtcggcgc
     7261 ccggatggga aaccgtgctg acgccgaagc cgacaaaagt taatgcaatc aggcctaata
     7321 agacttgggc gggtgttctg tatttttcga tggaatggaa catattttca gattgggaat
     7381 gggaaattca agtcgggtat tgtaacggtt tttatccctg tctgcacggg gcttgccggt
     7441 tgaagatgcc gtcgtaggtt tcttcgctga agccgacgta aaccctgctg ccgcactcca
     7501 atacgggacg cttgatcagg ctcggcattt cggacatcag tttgacggcc tccgccgtcg
     7561 aggacagggc tttttgctgt gtttcggcat cgagtttgcg ccagcttgtc ccgcgtttgt
     7621 tgaacagggt tgccaaaggc acttgttcca gccacgagca gatttccgct tcagacggca
     7681 tctgtttttt gaaatcccga aacccaaact ccaagccgta tccggcaagc ctgtttttgg
     7741 cttttttgac tgtgtcgcaa tttggaatgc cgtgaaggat tatcatttgg aaaccttttg
     7801 cctgaaataa taaaaccgat attttactat aagtgtctga aaatttgccc gtctgtttca
     7861 gacggcgggg cggttatgtt acaatccgaa aattcgaaaa atttaatctc ttgttcaata
     7921 aaggttttac ccataatgat ttctaccaac ggcattacca tgcagttcgg cgcgaagccg
     7981 ctgtttgaaa acgtatccgt aaaattcggc gaaggcaacc gctacggctt gatcggcgcg
     8041 aacggttcgg gcaaatccac cttcatgaaa atcctcggcg gcgatttgga acagaccgcc
     8101 ggcgaagtgg cgattgaaaa cggcgtgcgt ttgggtaaat tgcgccaaga ccagtttgcc
     8161 tacgaagaca tgcgcgtgct ggacgtggtg atgatggggc ataccgaaat gtgggcggcg
     8221 atgaccgagc gcgatgcgat ttacgccaat cccgaagcca ccgaagacga ctacatgaaa
     8281 gccgccgaac tggaagccaa gttcgccgaa tacgacggct acaccgccga agcgcgcgcc
     8341 gccgaattat taagcggcgt gggcatttcc gaagatttgc acaatgcgaa aatggcggaa
     8401 gtcgccccgg gcttcaaact gcgcgtattg ctggcgcagg cgctgttctc caagccggat
     8461 gtattgctct tggacgaacc gaccaacaac ttggacatca ataccatccg ctggctggaa
     8521 ggcgtgttga accaatacga ctccaccatg atcatcatct cgcacgaccg ccacttcttg
     8581 aacgaagttt gcacccacat ggcggatttg gactacaaca ccatcactat ttatccgggc
     8641 aactacgacg actatatgct cgcttccgcc caatcgcgtg agcgcgccct gaaagacaat
     8701 gccaaagcca aagagaaact gcaagagctg caagaattcg ttgcccgttt ttccgccaat
     8761 aaatccaaag cccgtcaggc aaccagccgt ctgaaacagg ccgacaaaat caaatcggaa
     8821 atggtcgaag tcaaaccttc tacccgtcaa aacccgtata tccgttttga agccgatgaa
     8881 aaagccaagc tgcaccgtca ggctgtggaa gttgaaaaac tggccaaacg ctttgaaacc
     8941 cagttgttta gaaacctcaa cttcatcctt gaagcaggcc aacgcctcgc catcatcggc
     9001 ccgaacggcg caggtaaatc taccttgctg aaactcttgg ccggcgcgta caaccccgaa
     9061 tattcagacg gcctgttgcc ggacgaaggc agcatcaaat gggcggaaaa agccagtgtc
     9121 ggctactatc cgcaagacca tgaaaacgac tttgacgtcg atatggactt gagcgaatgg
     9181 atgcgccaat ggggtcggga aggcgacgac gaacaagtca tccgaggcac tttggggcgt
     9241 ttgcttttcg gcagcaacga cgtggtaaaa aaagtgaaag ttctctccgg cggcgaaaaa
     9301 ggccgtatgc tttacggcaa actgttgctg ttgaaaccca atgtcttggt catggacgaa
     9361 ccgaccaacc atatggacat ggaaagcatc gaatcgctga acatggcact ggacaaatac
     9421 aacggcacgc tgattttcgt ctcccacgac cgtcagttcg tttcctccct ggcaacccaa
     9481 atcatcgaac tggacggcaa aggcggatat gaacactact tgggcgatta cgaaagctat
     9541 ctcgagaaaa aaggcgtagc ataagcgcac ccctccggtt gaaacaatgc cgtctgaagc
     9601 cgcttcagac ggcattgttg ataactttaa aataggaagc atatgcagac ttacctcgtc
     9661 ggcggcgccg tccgcgatta tcttttgggc ctgcccgtca aagaccgcga ttgggtggtc
     9721 gtcggcgcag acgcgcaaac catgttggcg caaggtttcc agccggtcgg caaagatttt
     9781 cctgtattcc tccatcccaa aacgcacgaa gaatacgccc tagcccgcac cgaacgcaaa
     9841 accgccaagg gttacgccgg tttcagtttc cacgccgaca aagacgttac gctggaacag
     9901 gacttgatgc gccgcgacct gaccatcaac gcgatggcgc aagatgcgga cggcaagatt
     9961 atcgaccctt tcggcgggca acgggatttg gcggcaggca ttttgcgcca cgtctcaccc
    10021 gccttcgccg aagatcccgt ccgcatcctt cgtgccgccc gctttgccgc gcgttacggc
    10081 tttgaaatcg ccgaagaaac cataaagctg atgcggcaga tggtggaaaa cggcgaagcg
    10141 gacgcattgg ttgccgaacg cgtctggcag gagttggcga aaggtttgat ggaaaaaaat
    10201 ccgcgcaaaa tgattgaaat gttgcgcgaa tgcggcgcac tccaagtctt gctgcccgaa
    10261 gtcgacgccc tcttcggcgt gccgcaacgc gccgactatc atcccgaaat cgacagcggc
    10321 atccataccc tgatgacgct gcaacgcgcc gccgatatgg gtttgagcct gcccgaacgc
    10381 tatgccgccc tgctgcacga cttgggcaaa gccaaaacac cgcccgacat cctgccgcgc
    10441 caccacggac acgacatcaa cggcgtagaa cccgtgcgcg aagtcaatca acggctgcgc
    10501 gcgccgaggc agtgtgccga gcttgccgaa ttggtttgcc gttggcacat tattttccac
    10561 caagtcggac agcttaaaag ccaaaccatt ctgaacgtat tgaaaaaaac cgatgccttc
    10621 cgccgtcccg agcgttttca gacggcattg aacgtctgca ttgccgacac gcaaggccgt
    10681 ctgaaccgag aacacacgcc ctacccccag cgcgcgcact ggctcgccct gctcgaagcc
    10741 gccaatcagg cggattcggg caaaatcgcc gccgaatgcc gcgcgcaggg aaaagcgcac
    10801 tttatcgccg aacaaatcga ccgggcgcgg ctggcgcaaa tcgccccgct gcaaaaagcg
    10861 tttcggggcg cgtaggaaaa agaaaacgcc aaacgcggca tcttttgagg ttcgcccgca
    10921 ttgccgaaac agcgtcggag aaagggatgg atgacatcgg tttattcggc tgccttttaa
    10981 tattcccttg atgcaggcct gattagtcgg acaaaaatac cttttgttca acggaaccga
    11041 tttctatccg gacggaaaca gccggctttc cccttgcaaa cggatggaat caagcgggta
    11101 ttcaaacaca gccgtttatt tttattccgc cgccaagtta tgggataggg tttgcgtgtt
    11161 tcgggcaggc aaaccgcgca accttagacg cagtccgaag ttttgaaggc ggcatccggg
    11221 ccgggtttta aaacagccgc cgccatatcc gctataatcc atccttcagc cattccgccc
    11281 ccgacataaa atcatgaccc tgaaaaccga tttattgcct aaaatcaaca acgaagatta
    11341 tcaacgcctc atcctcaaac atagcgcgga attcagcggc ggagaaaccc gcctgttgaa
    11401 cgaaatcctc gaaaaattca atttcgacgt tgttcaggcg caggcattgg cgcaggcggt
    11461 aatgcagcaa gttcgcttcg atcccaacgc ctaccacatc gacagcgacg acgaagacac
    11521 caccggcatc tgcccccact gcatcaaccc gcctatgccg cccctgcgcg actatctcgt
    11581 ttggcgcgaa acacgcggat aaaacgcttt tgaccgttat cttttcaatg ccgtctgaaa
    11641 cgccgccgac cgttcggacg gcatacccga caaagggaac actatgctgc aaaccgacaa
    11701 cctgaccgcc gcgcaaccgc aacgcatcgt tgccgcccaa accgcctccg cacaggaaga
    11761 actgctcgaa cgcgccctcc gccccaaaac gctggacgac tacatcgggc agcataaagc
    11821 caaagagcag ctcgccatct tcatccaagc cgccaaaaaa cgcggcgaag cactcgacca
    11881 cgtcctgctt ttcggcccgc ccggactggg caaaaccacc cttgcccaca tcatcgccaa
    11941 agagttgggc gtaaatttgc gccaaaccag cggccccgtc ctcgaacgcg ccggcgacct
    12001 cgccgccctt ttgaccaacc ttgatccgca cgacgtattg tttatcgacg aaatccaccg
    12061 gctaagcccc gtcgtcgagg aaatcctcta tcccgcgctc gaagactacc ggctcgacat
    12121 tatgataggc gaaggacccg ccgcacgttc cgtcaaaatc gacctgccgc ccttcaccct
    12181 cgtcggcgcg accacccgcg ccggtatgct gaccaacccg ttgcgcgacc gcttcggcat
    12241 cgtctcccgc ctcgagtttt acgaaaaccg cgaccttacc accatcgtca gccgttcggc
    12301 acaactgttg cagctcgata tgggcgaaga aggcgcgatg gaagtcgcca aacgcagccg
    12361 cggcacgccc cgcatcgcca accgcctgtt gcgccgcgtg cgcgatttcg ccgacgtgaa
    12421 aaacaacggc gtaatcgatg ccgccgtcgc cgatgccgct ttaagtatgc tggacgtgga
    12481 cgcgcagggg ctggacgtga tggacaggaa attcctcgaa gccgttttgc acaaattcgg
    12541 cggcggcccc gtcggtttgg acaacgttgc cgccgccatc ggcgaatcta cggacaccat
    12601 cgaagacgtt atcgaaccct acctcatcca acaaggcttt ttgcaacgca ccccgcgcgg
    12661 caggatggcg accgaacgcg cctacctgca tttcgggctg cccgtcgaaa aataaaccat
    12721 gccgtctgaa accgaaccgg gtttcatacg gcattgttat cagacgtttg caccggcacc
    12781 ggtttcatca cttaacagaa accttttgtc cggcaggaca gattctcccc gatgaaaata
    12841 actgtttccc tggcaaacgg ttgaaacccg tctggctgcc ggttgaaaaa taacgcaatg
    12901 ctgtctgaaa cagagctaat tttcagacgg catttctatt tcaatcattg gctcaaggtt
    12961 cagcctgccg ctttttccag ttccgccctc atcgcagcga ttaccgcctt gtaatccggt
    13021 ttgccgaaaa tcgccgaacc ggcaacaaag gtatccgcac cggctcgggc aacggcggca
    13081 atattgtcag ttttgatgcc gccgtccact tcaatggcaa tacgtctgcc gcttttccct
    13141 tcatagcggt caagcatggc gcgaacctgt ctgatttttt ccaaagtgtg cggaatgaag
    13201 ctctgaccgc cgaacccggg gttgaccgac atcagcaaaa ccatatccag cctgtccaac
    13261 acgttttcca acacatatac gggcgttgcc ggattcaaca ccagccccgc ctgacagccc
    13321 atgtcccgaa tcaggctcaa gctgcggtcg atatggcggc tcgcttcggg atggaaagtg
    13381 ataatcgatg ctcctgcttt ggcaaacgat tgaattaaat cgtcaacggg ttcgaccatc
    13441 agatgcacat cgatcggcac gcttgcataa ggcttcaacg ccgcgcagac cataggtccg
    13501 aaggtcaggt tcggcacata atggttgtcc atcacgtcaa aatggatcag atccgcaccc
    13561 gccgcaatga catttgccac ctcttctccg aggcgggcaa agtctgccga caaaatactg
    13621 ggtgcgatac ggtaggcggt catatttttt ccttcaatgc ctttatgggc ggactcaacg
    13681 cgggaaagcc tcgaaacagc attattaaca aaagttaact gccataatac catctttcac
    13741 acggcaatca agtatattgt ccgaaatatc cggattaaag gaaaagccgt gccgctgcct
    13801 gctccctgcc gttttgccaa acctgccgcc tcttttttaa gtatggcttt gctttcctgc
    13861 cagctttccc acgccgccac ggcttatatc cccccgaacg attttcaacc gaactgcgac
    13921 atacgccggc tcgggctgac acagggtcag cacaatgagc tgcgtaaaat ccgcgccgcc
    13981 ttcaaaatgg cgggcgacag ggcgcgtttg aaggttatgc attccgaaca cagccgccgc
    14041 cgctctgtcg tcgaaatcat ttcttcggat gtttttaatc ggaacgaggc gcgcgattat
    14101 gtcgaaagcc gctaccactc cagcatggat tttgcggtgg acgaattgga aatccaacac
    14161 cgcttcttcc atattctcac accgcaacag cagcaaatgt ggctttcttc ctgcctcaaa
    14221 taatccccga aacactcacg gcgcacgttg ctccggcagc ctgcccgccc agtcgcaggc
    14281 aaactgccaa gcggaacgtc ccgagcggtt gccccgcatc tgcgcccact gcaacgccgc
    14341 cgtccgggcg gcttcgtcat atggcacatc aaaatcttcc aaccagcttc gcactgccgc
    14401 cagataatcg ttttgatcga acggataaaa actgagccac aatccgaatc ggtcggacaa
    14461 agatactttt tcttccacgg cctctttttg atggatttct ccgcgcacgc ctgtcgtacc
    14521 tgcattttca tccaaatatt cgggcatcag atgccgtctg ttggaagtcg cgtacaccat
    14581 cacgttggcg caacgttggg acagaccgcc atccaatgcc gttttcaatg ccttataggt
    14641 ttcgtcgccg ctttcaaatg acaaatcgtc gcaaaacaca atgaattttt ccgggcgttc
    14701 cttcaaaagt gtcaacagat acggcaggcc gatcaaatcg cttttatcga cttcgatcag
    14761 gcgcaatccc ttatccgcat attcgtgcag cagggctttg accagcgagg atttgcctgt
    14821 tccgcgcgcg ccgctcatca atacgttatt cgcaggtctg ccggcaataa attgttcggt
    14881 attacgcacc agcaattcgg tctgcctgcc gactccggca agccgcgaca aagggaaaac
    14941 gtgcggatcg ggcaagtgtt ccaaaaaacc ttttttgccc gcactctgcc agcggaaggc
    15001 aagcgcgttc caatccgtat gcccgggttc gggcggaagc acggcatcca aacgccgcaa
    15061 aacggcatag gctttatcga gaaattcgtt caattccatc tctacctcac tttgcatatc
    15121 tttgcgccat cagccgttcg acggtgtcga cgattgcctg cgtattcgga tcgatttcga
    15181 tgttgatcct gtctccggcc tttctgctgc cgaacagcgt ccgttccaaa gtttcgggaa
    15241 tcagatggac attgaaacgg ctgtcttcga ctttgcctat ggtcaggctg caaccgtcca
    15301 agccgacgaa ccctttggtc aggatatagg gtttgagttc gtgcgggagc gcaaaccaaa
    15361 ccgtgcggtt gaacccgtcc cgttcgattt cgacaatggg cacggttgcc ataatgtgtc
    15421 cgctcatgac gtgtccgccg atttcgtcgc cgaaacgtgc cgcccgttcg atgttgacgc
    15481 aatcgccttc cttcagcagc cccaaattgg tttttgccaa agtttctgcc attaaatcga
    15541 agctgacgcg gtttccttcg atttcagtaa tcgtcaggca gcagccgtta ttggcgaccg
    15601 atgcgccgtg ttgcaggttt tctgccgcct cttgcggaag ctcgacgaca taagtctgaa
    15661 acgcctccga cgggcggtgg attgccgtca gttttcccaa tccttgaaca atgcctgtaa
    15721 acataatcct gtttccctgt gtcggtaaaa atggtgcaaa ttgtagcatt tccccgcgaa
    15781 aaatgccgtc tgaaactcct tcagacggca ttgtgccaaa aaccgcccgg tacgccttga
    15841 cctttccttt ccgcgccggt cggcggtctt gcacttatcc ctcctgcaaa tcggtttgcg
    15901 tgttcaagtc ggcaaaatgc ccgtcaaact cgaatctgac cggccgcgcc ctttgctgct
    15961 gcaaccagct tcttaatgtt ttcatacccg aaaacagata aggaatcgcg ctttgcagaa
    16021 tttgcggacg gatatacata atgttgtagt gcatcgttac cggcgtttcc acataaaacg
    16081 cattgcacaa cggtgtgcgt ttggagacgc tttcaaagcg cgcgaccaaa tcgtccggca
    16141 gatacggcat atcgcaaggt acaatcagca gccaatcggc cgccgccaac tgcaaatcgt
    16201 tggctgcggt acacaatgcc gaaagcgggc cgaaatgctg ccactgccgg gcatccggaa
    16261 aaacatgcgg gctgcggcgg gcgtattctt ccaaattccg gttggcactg atgacgatat
    16321 ggctgacctg cagcctgatt ttaccgataa cgcggtcaat cagcggtctg ccttccaaca
    16381 gtgccagccc tttgtcttcc ccgcccatac ggtcggccag gcctcctgcc aaaatcaatg
    16441 cgaacgtttt taatgcgggc gggcttttgg gaaagtttga ggttttcata gtttcagtct
    16501 gccgtttccg atattttaga agcgcgtttg aaaacggcgt cgtataaatt catgttattt
    16561 gaattttatc ataacgtgat ttaacactga aagcattgag gcttaagttt tccttaacct
    16621 gtcctttctg agcagttgtt tctaattcca aagaatgata ttgtttgcac cgtttaaaac
    16681 gactttcctc cgaatatgat actgccaacc cgattttcag acggcatccc cctttccctg
    16741 cgcctgaaac tcctgaccgg cttgtgggtc ggtttggcgg cactgtctgt cgttttaact
    16801 ctgctgcttt ctttccgcct ggaaaatgcc gcctcggtca tcgaagaggc gggcaatctg
    16861 aagatgcagg cataccggct tgcctatatg gcgggcgaag gctcgccccg cgcacaaatc
    16921 gacaatcaga ttgccgaatt tgaaaaaagt ttaaaacgca tttcccaaag cgatgccatc
    16981 catccgctga ttccctccga caatcccctt gcctacgatt tgatacagtc catgctgatt
    17041 atcgactggc aggccaacat cctccccccg ttgcaggcat accgccgccc cacacaaatc
    17101 gagctttacc gctttgccgg caatatcgaa ctgtttttgc aggcgttgga aaatgccggc
    17161 gaaaaaaaca cttggtggct caggcggttt caatgggtca ttatgctgat gacgctggtg
    17221 tcgtccgtgc tgatgctttt ttggcaccag atttgggtta tccgtccgct gcaggcgtta
    17281 agggaaggtg cggaacgcat cggtcagcgg catttcgaca tccctgttcc cgaagacggt
    17341 acgcccgaat tcaaacaggt cgggcggtgt ttcaaccaaa tggcgctcag gctgaagact
    17401 ttatacgatg atttggaagg acaggttgcc gaacaaaccc acaaccttga aaagcaaaac
    17461 cgcaacctga ccctgctgta ccggaccacg cgcgacctgc atcaatccta tacgccgcga
    17521 caggcggcgg aagaattttt gaaccacatc ctgcccgccg tcggcgcgca atccggcaac
    17581 atctgcctgg aaaacggttc cgacacagac atttccgtcc atacggccga acacggcaaa
    17641 aagccccctt tggaaaaata ccatgacgaa accttcccca tcgaatatca aaacgagaaa
    17701 ctgggcatgc tttcgctcgg cttttcagac ggcacttccc tcaccggaga cgaccgcacg
    17761 ctgcttcaaa ccctgattcg gcaattgggc gtatcgctcg ccggggcgaa acaggaggaa
    17821 gaaaaacgtc tgcttgccgt attgcaggaa cgcagcctga ttgcacaggg gctgcacgac
    17881 agcatcgcgc aggcattaac cttcctgaac ctgcaggtac agatgctgga aaccgccttt
    17941 gccgaaaaca aacgggagga agccgcagaa aacatcggct ttatcaaaac aggcgtgcag
    18001 gaatgttatg aagatgtccg cgaactgctg ctcaatttcc gtaccaaaat cagcaataaa
    18061 gaatttcccg aagctgttgc cgacctgttc gcgcgcttca cgcaacaaac cgggataacg
    18121 gtcgaaaccg tttgggaaaa cggttcgttc ctgcctacac aggacgagca gctccaaatg
    18181 attttcatcc tgcaagaaag cctgtccaac atccgcaaac acgcccgcgc cacccatgta
    18241 aaattcaccc tttccgaata cggcggacgc tttaccatga ccatccaaga caacggacaa
    18301 ggtttcgaca cggagaaaat aggagaacct acgggcagcc atgtcggact gcacatcatg
    18361 caggagcgtg ccaaacgcat ccgtgccgtt ttagaaatcc gttcccaagc acaacaggga
    18421 accaccgtct cattgacggg cgcacccaaa gaaagcctac catgaccatc aaaatcatcc
    18481 tgatagacga ccacaccctc ttccgcagcg gcatcaaagc ccttttgtcg cgccaacacg
    18541 gttttgaagt catcggcgaa gccgcagacg gactctcggg catcaaaatg atcagtcggc
    18601 tgcaacccga tgtcgtcctg ctcgatttgg atatgccagg catgaacggg cgcgaggcct
    18661 tgtcccaaat catcagcatc aatccgcagc aggcagtcat tatgctgacc gtttccgaag
    18721 acagcgacga ccttaccgaa tgtatgcgca tcggcgcgcg cggctacctg ctgaaaaaca
    18781 tcaacgccga ttttcttctc gaaagcatac gcaaagccgc cgaaggcgat aatgtattct
    18841 cgcccgagat gaccgccaaa ctcgtcaaaa gcctgatttc cccccaacct gcccaaagga
    18901 cgcaggcact ctcctcactt acccctcgtg aactggaaat cttgggctat ctcgccgcag
    18961 gacacagcaa caaaatcatc gcccgccacc tcgatcttgc cgaatccacc gtcaaagtcc
    19021 acgttcaaaa cctgctccgc aaactcaacc tcagcagccg ggtgcaggcc gccgtttacg
    19081 ccatccggca caacgtcccc caacctgtgc cggaatagac gttcagacgg catcgggtca
    19141 ggaaaaaagc aatgcgcccc tttgacgggg tgcaatatat aagggacggc acaccggatg
    19201 ccgtcccttt aagtacgcca ttaccgccgt aacccggacg gaatggctta ggcagtttat
    19261 ttccaagaat atttcacacc cacggtgcct tggtagcctt tatgtctgtt gcttccgcat
    19321 cctgccgata attgccttgc ctgtcccgag aaagggtttg attttcgcca cggcgttggt
    19381 ggagcgtatc cagcatcgca tagcctgatt ccaagttttg acgtggaacg agtgcgtagg
    19441 ccggtgcgct tggcgtgatg atggttttat cttgattcgg cgtggttaaa gtccagaagt
    19501 attctgtcgc gcccgcctca tctttgtgtg aaaccaattg caattccccc gcccctgtgg
    19561 ttttggcagt tccgctaaaa gtatcgcgca ttgtgtaggt atgtttagcg tcttcgatat
    19621 ttaatttacc ggtttccgcc cccttgtctt gtcccaaaac cttaacaacg gcggcactgc
    19681 gtttgtagcg gtcggaaagt tcgccgatcg agccgtcgat aatattttct ttgccatctt
    19741 tacccactga aatgaccgtt gtttttcctg aagcatcgcc tgtgatttcc agtaaatcag
    19801 attgagaatt tcctccttga tcatcacccg gcgagttcca ttctgtattg acttttaaca
    19861 caccgtcttt accgacataa tttccttcaa tggtgagttt gtctgcatag gattgattgg
    19921 caagcgtaat gatgccgccg ttggtgaatg ttcctttttc aaatacagaa gttgtacccg
    19981 ataaagaaat acctgtatta gatagggtta gcgtgccgcc tgataataat gtagtccctt
    20041 cgggggttgt taatgatgtg tcttttttta ggtgaaaagt agcttttgtt agttttatgg
    20101 ttccaatatc ttctgatttg aattccattg cgccatttgt aatttttagc acagcattgg
    20161 taatagggag ggggaagcgc attgtaaatg atttttctgt tccatctgga ttgattaatg
    20221 taccagatgc gccaaagatc tctccttcag ggatagaaac agttccatca gaaattgatg
    20281 gtttatttgc gcttttatca ctgtgtttat gaattaatgt aattggggct gtactagatt
    20341 atccctaaat tccacaccga tcccgcagga tttttagctg ccgggacggt gtgccgaagt
    20401 taagtcgaaa ttcgc
//
LOCUS       DBJGGI010000032        20105 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.032, whole genome shotgun sequence.
ACCESSION   DBJGGI010000032 DBJGGI010000000
VERSION     DBJGGI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20105
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.032"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            233..1297
                     /locus_tag="ACSIGJ_001192"
     CDS             233..1297
                     /locus_tag="ACSIGJ_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HHQ1330058.1"
                     /translation="MKAARFYNKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPGANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDKLVSEG
                     FERLIHNNESAVKIIVNPNL"
     gene            1438..1761
                     /locus_tag="ACSIGJ_001193"
     CDS             1438..1761
                     /locus_tag="ACSIGJ_001193"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HHQ1330059.1"
                     /translation="MGDSVLSAIQQTIIQRKSADPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKDPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELSRRQGLSGLAEKA
                     ARTES"
     gene            1835..2158
                     /locus_tag="ACSIGJ_001194"
     CDS             1835..2158
                     /locus_tag="ACSIGJ_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HHQ1330060.1"
                     /translation="MDNCIFCKIAAKEIPAQTVYEDGEMVCFKDINPAAPLHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKASGLTDGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2205..2408
                     /gene="tatA"
                     /locus_tag="ACSIGJ_001195"
     CDS             2205..2408
                     /gene="tatA"
                     /locus_tag="ACSIGJ_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HHQ1330061.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2412..3098
                     /gene="tatB"
                     /locus_tag="ACSIGJ_001196"
     CDS             2412..3098
                     /gene="tatB"
                     /locus_tag="ACSIGJ_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HHQ1330062.1"
                     /translation="MFDFGLGELIFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQELDTQIELEELRKVKQAFEAAAAQVRDSLKETDTDMQNSLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPLPDTANTVSDGISDVMPSERSDTSAETLGDDRQTGSTAEPAE
                     TDKDRAWREYLTASAAAPVVQAVEVSYIDTAVETPVPHTTSLRKQAINRKRDFRPKHR
                     AKPKLRVRKS"
     gene            3111..3881
                     /gene="tatC"
                     /locus_tag="ACSIGJ_001197"
     CDS             3111..3881
                     /gene="tatC"
                     /locus_tag="ACSIGJ_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HHQ1330063.1"
                     /translation="MSETQNEQTVQPLVEHLIELRRRLMWMVVGILVCFFGMMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLVSLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLAGVTPVGVNMATDIDKYLS
                     FILGMFVAFGTAFEVPIVVILLTKIGAVTTEQLKHARPYVIVGAFVVAAVITPPDIIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPAKT"
     gene            3925..4533
                     /locus_tag="ACSIGJ_001198"
     CDS             3925..4533
                     /locus_tag="ACSIGJ_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HHQ1330064.1"
                     /translation="MNTLYLGSGSPRRMEILTQLGYRVVKLPAGIDETVKAGETPAPY
                     VQRMAEEKNQAALTLFCETNGAMPDFPLITADTCVFSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMEKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRPPLSALKP"
     gene            4542..4895
                     /locus_tag="ACSIGJ_001199"
     CDS             4542..4895
                     /locus_tag="ACSIGJ_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HHQ1330065.1"
                     /translation="MNRQTAYFLASFSLIALIALSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEKICAALSAVFSIIFFIS
                     CLSFVKQYKETNNVR"
     gene            4885..6699
                     /locus_tag="ACSIGJ_001200"
     CDS             4885..6699
                     /locus_tag="ACSIGJ_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HHQ1330066.1"
                     /translation="MSAETTVSGARPAAKLPIYILPCFLWIGIIPFTFALRLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWWLQARLMNLIYPGMNDI
                     ASWVFILLAVSAWACKSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWENTPL
                     LQNIIVHRGQGVIGHIGQRNNLGHYLMWGILASAYLNGQRKIPAALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNAILETF
                     TGIRYETAVERVANGGFTDLPRQSEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     TIHDNFLSTLFTHSHNIILQLLAEMGISGTLLVAATLLTGIAGLLKRSLTPASLFLLC
                     ALAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     ELLHLDWTYTRLVNSFSPAADDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKALKYRPYSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPETKPCK"
     gene            6889..7566
                     /gene="misR"
                     /locus_tag="ACSIGJ_001201"
     CDS             6889..7566
                     /gene="misR"
                     /locus_tag="ACSIGJ_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator MisR"
                     /protein_id="HHQ1330067.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7579..8985
                     /gene="misS"
                     /locus_tag="ACSIGJ_001202"
     CDS             7579..8985
                     /gene="misS"
                     /locus_tag="ACSIGJ_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     MisS"
                     /protein_id="HHQ1330068.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFKTRGDNGAREILTEWKNSPVSSAVYVIQGDEKKDILNRYIDNYTIE
                     RARLFAANNPHSNLVRIEYDRFGEEYLFFIKGWDNHQAQRLPSPLFIPGLPLAPIWHE
                     FIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMAEKLEKLVAKERYLLHHVSHEMRSPLARMQAIVGLIQAQPQKREQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVAL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGFKTEKSAN"
     gene            9103..11526
                     /locus_tag="ACSIGJ_001203"
                     /pseudo
     CDS             9103..11526
                     /locus_tag="ACSIGJ_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            11571..11816
                     /gene="rpsP"
                     /locus_tag="ACSIGJ_001204"
     CDS             11571..11816
                     /gene="rpsP"
                     /locus_tag="ACSIGJ_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HHQ1330069.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAV"
     gene            11832..12341
                     /gene="rimM"
                     /locus_tag="ACSIGJ_001205"
     CDS             11832..12341
                     /gene="rimM"
                     /locus_tag="ACSIGJ_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HHQ1330070.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLA
                     KDGKTVSVTLEAGKVVNGELQVKFEGIDDRDSAFSLRGYTIEIPREAFAPTEEDEYYW
                     ADLVGMTVVNKDDTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            12341..13090
                     /gene="trmD"
                     /locus_tag="ACSIGJ_001206"
     CDS             12341..13090
                     /gene="trmD"
                     /locus_tag="ACSIGJ_001206"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HHQ1330071.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQTAKVIYLSPQGKPLTHQKAAEL
                     AELTHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGIL
                     GDIQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLNKILQEQREIQS"
     gene            13105..13470
                     /gene="rplS"
                     /locus_tag="ACSIGJ_001207"
     CDS             13105..13470
                     /gene="rplS"
                     /locus_tag="ACSIGJ_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HHQ1330072.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13791..14516
                     /locus_tag="ACSIGJ_001208"
     CDS             13791..14516
                     /locus_tag="ACSIGJ_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HHQ1330073.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTSQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TESYLAQASAKMQQQESPDWCAS"
     gene            14551..15426
                     /locus_tag="ACSIGJ_001209"
     CDS             14551..15426
                     /locus_tag="ACSIGJ_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HHQ1330074.1"
                     /translation="MNLYDLLLAPFAEFDFMHYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGVGGFAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVILISKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSGLTLITLAVIY
                     RPLVLESIDPLFLKSVNGKGGLWHVVFLILVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWARNMGTLILLSVLIALFCGLIGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILPKWFKNHRHHTT"
     gene            15503..16417
                     /locus_tag="ACSIGJ_001210"
     CDS             15503..16417
                     /locus_tag="ACSIGJ_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter solute-binding protein,
                     Zn/Mn family"
                     /protein_id="HHQ1330075.1"
                     /translation="MKHLKLTLITALLATAATAAPLPVVTSFSILGDVAKQIGGERVA
                     VQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADIQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEYDPHVWNDPVLMSDYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGNRYNISFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNVKALTNAMKQ"
     gene            16637..17503
                     /locus_tag="ACSIGJ_001211"
     CDS             16637..17503
                     /locus_tag="ACSIGJ_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HHQ1330076.1"
                     /translation="MKNILLVFVSFVPLCVRTDLPLNIEDIMTDKGKWKLETSLTYLN
                     SENSRAALASPVYIQTGSASFIPVPTEIQENGSNTDMLAGTLGLRYGLTGNTDIYGSG
                     SYLWHEERKLDGNGKTRNKRMSDISAGISHTFLKDGKNPALIAFLESTVYEKSRNKAS
                     SGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDDVKYKAGNYWMLNPNISFAAND
                     RISLTGGIQWLGKQPDRIDGKKESARNTSTYAHFGAGFGFTKTAALNASARFNVSGQS
                     SSELKLGIQHTF"
     gene            17546..18085
                     /locus_tag="ACSIGJ_001212"
     CDS             17546..18085
                     /locus_tag="ACSIGJ_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330077.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAGGLGAIPGDV
                     GAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQGI
                     GRHRPWESKSTDRSWKNRF"
     gene            18067..18432
                     /locus_tag="ACSIGJ_001213"
     CDS             18067..18432
                     /locus_tag="ACSIGJ_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330078.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMPSDDLFLKCLQSSYKAYSETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            18490..18867
                     /locus_tag="ACSIGJ_001214"
     CDS             18490..18867
                     /locus_tag="ACSIGJ_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330079.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGEWLKHIYEFNRESFIFSDVLLQKGESEENFSEIVLKSIKNN
                     KILTVEVRSGLHFDLRNIYRIEM"
     gene            18912..19250
                     /locus_tag="ACSIGJ_001215"
     CDS             18912..19250
                     /locus_tag="ACSIGJ_001215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330080.1"
                     /translation="MKTFEKTWSAQYRDMEISVRNFWNLKRTGAEVYINGRRVYHNEA
                     GMASASLRSLMGEYLEFEESGTKITVEIGSAWHFNETIRNINTHLRGTKLPGSNWRVQ
                     DKGHFHLWKR"
     gene            19238..19786
                     /locus_tag="ACSIGJ_001216"
     CDS             19238..19786
                     /locus_tag="ACSIGJ_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330081.1"
                     /translation="MEKIINSKVRETNFNQAWRVIRAMQINNISDEQVMIVKMSCYLK
                     KDEIFKNIERYGYQSSDIKKISPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKPGETLYLNFKSIKNQCKNYFFAVLVLMPLTLKAVR
                     VKWQIIILFLMPFLYSLFNQII"
     gene            19806..19997
                     /locus_tag="ACSIGJ_001217"
     CDS             19806..19997
                     /locus_tag="ACSIGJ_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330082.1"
                     /translation="MLPACSVSSLLLGEYLEFEENGTKIAAEIGSAWHFLGAACRISI
                     NGKYYAGNRIVWFAKKAES"
BASE COUNT         5395 a         5933 c         4430 g         4347 t
ORIGIN      
        1 ttaaggggat attcgggtaa aattaggagg tatttggggc gaaaacagcc gaaaacctgt
       61 gtttgggttt cggatgtcgg gagggaaggg aattttgcaa aggtctcagt ttgtagtaaa
      121 atatctaaac agattcaatc ggaaacggtc aggcttcttc acgccattcc catttcaacc
      181 gccgcctgct agattcagac ggcattccta cttgaaaaca aggagcgtta caatgaaagc
      241 agcacgtttt tacaacaaag gcgacatccg catcgaagac atccccgaac cgaccgtcgc
      301 ccccggcacc gtcggcatca atgtcgcctg gtgcggcatc tgcggtaccg acctgcacga
      361 atttatggaa ggcccgattt tcatcccgcc ctgcggtcat ccgcatccga tttccggcga
      421 gtccgcgccc gtaacgatgg gacacgagtt ctccggcgtg gtttatgccg tcggcgaagg
      481 cgtggacgac attaaagtcg gccaacacgt cgtggtcgaa ccctacatca tccgcgacga
      541 cgtaccgacc ggcgaaggca gcaactacca cctctccaaa gacatgaact ttatcggctt
      601 gggcggctgc ggcggcggcc tgtccgaaaa aatcgccgtc aaacgccgtt gggtgcatcc
      661 gatttccgac aaaatcccgt tggatcaagc tgctttgatt gaacctctgt ctgtcggaca
      721 ccacgcctat gtacgcagcg gcgcgaaagc aggcgatgtc gcattggtcg gcggcgcagg
      781 cccgatcggt ttgctgttgg ccgccgtgtt gaaagccaaa ggcatcaaag tcatcattac
      841 cgagttgagt aaagcacgca aagacaaagc ccgcgaatcc ggcgttgccg actacatcct
      901 cgacccgtcc gaagtcgatg ttgttgaaga agtgaaaaaa ctgaccaacg gcgaaggcgt
      961 ggacgtggcg ttcgaatgta ccagcgtgaa caaagtgttg gatactttgg tcgaagcctg
     1021 caaacccggc gccaacttgg ttatcgtatc catctggagc caccccgcca ccgtcaacgt
     1081 ccacagcgtc gtgatgaaag aactggatgt gcgcggcacg attgcctact gcaacgacca
     1141 cgcggaaacc atcaaactgg tcgaagaagg caaaatcaac ctcgaaccct tcatcaccca
     1201 gcgcatcaag ctggacaaac tggtttccga aggcttcgag cgtctgatcc acaacaacga
     1261 atccgccgtt aaaattattg tgaatccaaa cctgtaagca aattacattt gcaacagcac
     1321 aaatgccgtc tgaaacgctc agacggcatt ttccaacgaa acgcaacagg cgtattgccc
     1381 gagagcagcc gtttagccta tcattccgaa caaacagaaa caaacagaag gaaaatcatg
     1441 ggagattccg tactatccgc catccaacaa accattatac agcgcaaatc tgccgacccg
     1501 tccgaatctt acgtcgcaca gctcctgcac aagggcgagg acaaaatcct aaaaaaagtg
     1561 attgaagaag cgggcgaagt gttgatggca tccaaagaca aagacccgtc ccacctggtt
     1621 tacgaagttg ccgacttatg gttccacacc atgattcttc tgacacacca cgacctgaag
     1681 gcggaagacg tattggacga actttcccgc cgtcaggggc tgtcggggct ggccgaaaaa
     1741 gccgcccgta cagaatcctg aatttatatt acaatctcat cccttccacg tttaatccgt
     1801 ctgcgtgctt ttttcagacg gcaacggagc cgttatggac aactgtattt tctgcaaaat
     1861 cgccgccaaa gagattccgg cgcaaaccgt ctatgaagac ggcgaaatgg tttgtttcaa
     1921 agacatcaac cccgccgctc cgcttcatct gctgctgatt cccaaagtcc attttgattc
     1981 gttggcacac gccgcgcccg aacatcagcc ccttttggga aaaatgatgc tgaaagttcc
     2041 cgaaatcgcc aaagcttcgg gactgaccga cggtttcaaa acccttatca ataccggcaa
     2101 aggcggcgga caagaggtct tccacctgca tatacacatc atgggcacac ccgtataaac
     2161 cgttatttca caatcaaccc ctaatactta cttaaggata catcatgggc agtttttctc
     2221 tgacgcactg gattatcgta ctgattatcg tcgttttgat attcggcacc aaaaaattgc
     2281 gcaacgtcgg caaagacctc ggcggtgcgg ttcatgactt caaacagggg ctgaacgaag
     2341 gcacagacgg caaagaagcc caaaaagacg atgtaatcga acacaaaaaa gacgaagaca
     2401 aagcataact tatgtttgat ttcggtttgg gcgagctgat ttttgtcggc attatcgccc
     2461 tgattgtcct tggtccagaa cgcctgcccg aagccgcccg cactgccgga cggcttatcg
     2521 gcaggctgca acgctttgta ggaagcgtca aacaagaact tgacactcaa atcgaactgg
     2581 aagagctgag gaaggtcaag caggcattcg aagctgccgc cgctcaggtt cgagacagcc
     2641 tcaaagaaac cgatacggat atgcagaaca gtctgcacga catttccgac ggtctgaagc
     2701 cttgggaaaa actgcccgaa cagcgcacgc ctgccgattt cggtgtcgat gaaaacggca
     2761 atccccttcc cgatacggca aacaccgtat cagacggcat ttccgacgtt atgccgtctg
     2821 aacgttccga tacttccgcc gaaacccttg gggacgacag gcaaaccggc agtacagccg
     2881 aacctgcgga aaccgacaaa gaccgcgcat ggcgggaata cctgactgct tctgccgccg
     2941 cacctgtcgt acaggccgtc gaagtcagct atatcgatac tgctgttgaa acgcctgttc
     3001 cgcacaccac ttccctgcgc aaacaggcaa taaaccgcaa acgcgatttt cgtccgaaac
     3061 accgcgccaa accgaaattg cgcgtccgta aatcataaag ggggcatccg gtgtccgaaa
     3121 cacaaaacga acaaaccgtc caaccgcttg tcgaacacct catcgagcta cgccgccgcc
     3181 tgatgtggat ggttgtcggc attctcgtct gctttttcgg catgatgccg tttgcccagc
     3241 agctctatac ttttatcgcc gacccgctga tggcaaacct gcccaaagac accagtatga
     3301 ttgcgaccga tgtcatcgca ccgtttttcg ttccggtcaa agttaccctg atggcggcgt
     3361 ttttggtttc actgccgcat acgctctacc aaatctgggc attcgtcgcc cccgcactct
     3421 accaaaacga aaaacgcctg attacgccgc tcgtcctctc cagcgtcagc ctgtttttca
     3481 tcggcatggc atttgcctat ttcctggttt tccccgtcat tttcaaattc cttgccggcg
     3541 taacccccgt cggggtcaat atggcgaccg acatagacaa atacctgtcc tttatcttag
     3601 gtatgttcgt tgcgttcggc acggcatttg aagtgcccat cgtcgtcatc ctgttaacca
     3661 aaatcggcgc agtcacgacc gagcagctca aacacgcccg gccttatgtc attgtcggcg
     3721 cgtttgtcgt tgccgcagtc atcacgccgc ccgacattat ttctcagacc ctgcttgcca
     3781 ttccgctgat cctcttatac gaggcgggca tttggttcgg acgcttcttc actccacgtt
     3841 cagaacagga tggcgacata cagccgcctg caaaaacctg acactatgcc gtctgaagcc
     3901 ccgccgcaca ttaaggagca caccgtgaac accctttact taggttcggg cagcccgcgc
     3961 cggatggaaa tcctgacaca gttgggctat cgggtcgtca agctgcccgc cggcatcgac
     4021 gaaaccgtta aagccggcga aacgcccgcc ccttacgtcc aaaggatggc ggaagaaaaa
     4081 aatcaggcgg ctttgaccct cttttgcgaa accaacggcg caatgcccga tttccccctg
     4141 attaccgccg acacctgcgt cttttcagac ggcatcatat tgggcaaacc tcgctcccaa
     4201 gccgaagcaa tcgaattttt aaaccgattg tccggcaaac aacataccgt cctgactgct
     4261 gtctgcattc attatcgcgg caaaacgtca agccgcgtcc aaaccaaccg cgtcgttttc
     4321 aagcccctga gttcggaaga aatttccgcc tatgtgcaaa gcggcgaacc gatggagaaa
     4381 gccggtgcct acgccgtgca aggcataggc ggtatcttta tccaatctat cgaaggcagc
     4441 ttcagcggca ttatggggct gcccgtttat gaaaccgttt ccatgttgca ggatttggga
     4501 taccgccccc ccttatccgc ccttaaaccg taaagtccgc cgtgaacaga caaaccgctt
     4561 acttccttgc ctccttcagc ctgattgcac tgattgccct atccctttct tgggaattat
     4621 ggattgcacc gttacgcccg ggcggttcgt ggctcgcgct caaagccctg cccctctgcc
     4681 tgccgctttc aggtattcta aagaaaaaaa tctataccta ccaatacagc tccatgctgg
     4741 ttctgattta ctttgccgaa gccgtcatgc gcctgttcga cgcctatccc gcagaaaaaa
     4801 tttgcgccgc gctttccgca gtattcagca tcatcttctt catatcctgc ctgtcgttcg
     4861 tcaaacaata caaggaaaca aacaatgtcc gctgaaacga ccgtatccgg cgcgcgcccc
     4921 gccgccaaac tgccgattta catcctgccc tgcttccttt ggataggcat catccccttt
     4981 accttcgcac tcaggctgaa accgtcgccc gacttttacc acgatgccgc cgccgcggcc
     5041 ggcctgattg tcctgttgtt cctcacggca ggaaaaaagc tgtttgatgt caaaatcccc
     5101 gccatcagct tcctcctgtt tgcaatggcg gcattttggt ggcttcaggc acgcctgatg
     5161 aacctgattt atcccggaat gaacgacatc gcctcttggg ttttcatctt gctcgccgtc
     5221 agcgcgtggg cctgcaagag tttggtcgca cactacggac aagaacgcat cgttaccctg
     5281 tttgcctggt cgctgcttat cggctccctg cttcaatcct gcatcgtcgt catccagttt
     5341 gccggctggg aaaacacccc cctgcttcaa aacatcatcg ttcacagagg gcaaggcgta
     5401 atcggacaca tcgggcagcg caacaacctc ggacactacc tcatgtgggg catactcgcc
     5461 tccgcctacc tcaacggaca acgaaaaatc cccgcagccc tcggcgcaat ctgcctgatt
     5521 atgcagaccg ccgttttagg tttggtcaat tcgcgcacca tcttgaccta catagccgcc
     5581 atcgccctca tccttccctt ctggtatttc cgttcggaca aatccaacag acggacgatg
     5641 ctcggcatag ccgcagccgt attccttacc gcgctgttcc aattttccat gaacgccatt
     5701 ctggaaacct ttacaggcat ccgctacgaa actgccgtcg aacgcgtcgc caacggcggt
     5761 ttcacagact tgccgcgcca aagcgaatgg aataaagccc ttgccgcctt ccagtccgcc
     5821 ccgatattcg ggcacggctg gaacagtttt gcccaacaaa ccttcctgat caatgccgaa
     5881 cagcacacca tacacgacaa cttcctcagc accttgttca cccattccca caacatcatc
     5941 ctccaactcc ttgcagaaat ggggatcagc ggcacgcttc tggttgccgc aaccctgctg
     6001 acgggcattg ccgggctgct gaaacgctcc ctgacccccg catcactttt cctgctgtgc
     6061 gcgcttgccg tcagtatgtg ccacagtatg ctcgaatatc ctttgtggta tgtctatttc
     6121 ctcatcccct tcggactgat gctctttctg tcccccgcag aggcttcaga cggcatcgcc
     6181 ttcaaaaaag ccgccaatct cggcatactg accgcctccg ccgccatatt cgcagaattg
     6241 ctgcacttgg actggacata cacccggctg gttaactcct tttcccccgc cgctgacgac
     6301 agtgccaaaa ccctcaaccg gaaaatcaac gaactgcgct atatttccgc aaacagcccg
     6361 atgctgtcct tttatgccga cttctccctc gtaaacttcg ccctgccgga ataccccgaa
     6421 acccagactt gggcggaaga agcaaccctc aaagcactaa aataccgccc ctactccgcc
     6481 acctaccgca tcgccctcta cttgatgcgg caaggcaaag ttgcagaagc aaaacaatgg
     6541 atgcgggcaa cacagtccta ttacccctac ctgatgcccc gatacgccga cgaaatccgc
     6601 aaactgcccg tatgggcacc gctgctgccc gaactgctca aagactgcaa agccttcgcc
     6661 gccgctcccg gccatccgga aacaaaaccc tgcaaatgac ccgcgtcggc ggatgcggat
     6721 accgcccgaa atgtaaagct ccatgcaaga cattgcaaaa aacagcaaac cggtagggaa
     6781 atacgctatc aaaaaacatt gcagccgtgt taagataaac tgtcaaacaa tcttttcacg
     6841 ccacgcccga aacagggtcg gggcataccc ttacgaaaag gaaacaccat gagccgcgta
     6901 ttactcgtag atgacgatgc cctgctgacc gaactgctga ccgaatacct gagcgccgaa
     6961 ggtctgaacg tccgcagcgt tcccgacggg gaagcaggcg tacaggaaat cctgagcggg
     7021 caatacgatg tagtcgtatt ggattccatg atgcccaaaa tgaacggctt ggatgtcttg
     7081 aaaaacgtac gcgcccgaag caccgtcccc atcatcatgc tgaccgccaa aggcgacgac
     7141 atcgaccgaa tcatcggctt ggaaatgggt gcggacgact atgtccccaa accctgcaca
     7201 ccacgcgaac tcttggcacg catcaatgcc atcctccgcc gagcacaaca cagcggcgaa
     7261 cagaacaacg cacccaacag catctccgtc agcgatgtcg tcctataccc cgccaaacgc
     7321 caggcatccg tcaaagacat gccgctcgaa ctgaccagca ccgaattcaa cctgctcgaa
     7381 gtcctgatgc gccatgccgg acaggtagtc agcaaagaaa ccctgtccgt cgaagcactc
     7441 gaccgcaaac tggcaaaatt cgaccgcagt atcgacgtac acatctccag catccgccac
     7501 aagctgggcg atgcctccct gattcaaacc gtacgcggct tgggctacct gtttgtcaaa
     7561 aactgaagca gcgggcaaat gaaactgttc caacgcattt tcgccacatt ttgcgcggtt
     7621 atcgtctgcg caatctttgt ggcgagtttt tctttttggc tggtgcagaa cacccttgcc
     7681 gaaaaccaat tcaaccaacg ccgcaccatc gaaaccacat tgatgggcag cattatttcc
     7741 gcattcaaga cacggggcga caacggcgcg cgcgaaatcc tgaccgaatg gaaaaacagc
     7801 cccgtctcat ccgccgttta cgtcatacag ggcgacgaga aaaaagacat cttaaaccgc
     7861 tatatcgaca attacaccat agaacgcgcc cggttgtttg ccgccaacaa cccccattcc
     7921 aaccttgtcc gcatcgaata cgaccgtttc ggcgaagaat acctgttctt cattaaaggc
     7981 tgggacaacc accaggcaca acgcctgccc agcccgctgt ttatcccggg cctgccgctt
     8041 gccccgattt ggcacgaatt catcatcctc tccttcatca tcattgtcgg actgctgatg
     8101 gcatatatcc ttgccggcaa cattgccaaa cccatcagaa tcttaggcaa cggcatggac
     8161 agggtggcaa acggagaact tgaaacccgc atctcccaac aggtcgacga ccgcgacgac
     8221 gaattgtccc atcttgccat ccaattcgac aaaatggcag aaaaactcga aaaacttgtc
     8281 gccaaagaac gttacctact ccaccacgtt tcccacgaaa tgcgctcccc gcttgcccgt
     8341 atgcaggcaa ttgtcggact gattcaggca cagccccaaa aacgggagca atatctcaaa
     8401 cggctggaag gcgaactgac ccgcatggat acgctggccg gggaactgtt aaccctgtcc
     8461 cgcctcgaaa cctccaatat ggctttggaa aaagaaagcc tgaaactcct gcccttcctg
     8521 ggcaacctgg tagaagacaa ccaaagtatc gcccaaaaga acggacagac ggtcgccctg
     8581 tctgccgacg gaaaaatccc cgaaaacaca accatccttg ccaacgaaag ctacctgtac
     8641 cgcgccttcg acaacgtcat ccgcaacgcc gtcaactaca gtcccgaagg cagcaccatc
     8701 ctgatcaaca tcggacaaga ccacaaacac tggataatcg acgttaccga caacggcccc
     8761 ggcgtggacg aaatgcagct cccgcacatc ttcaccgctt tctaccgtgc agactccagc
     8821 gccaacaaac ccggaacagg actggggctt gcattgaccc aacatattat tgaacagcac
     8881 tgcggcaaaa tcatcgccga aaacatcaaa ccgaacggtc tgcggatgcg ctttatcctg
     8941 cccaagaaaa aaaccggttt caaaacagaa aaaagtgcga actgaccata atgccgtctg
     9001 aacccgcttc agacggcatt gcacaaacag ttaccccaaa acaaacacac agcgggtgca
     9061 tctccaaaaa tcacgcacat accgtccgca aaggaatata tcatgccggc acaaaccgat
     9121 ccgggctact tcttcatgcc caaccacatt atcctgatag gcgcgagcga acaaccgtac
     9181 agcctgggtg aacgcgtact cagcaacctg ctgagtacgc cctttcaagg aaaaatcacc
     9241 cccgtaaacc cgcgccacca caccatagcc gggcttcccg cctacaccag cctcaacaaa
     9301 atccccggca gtgcagacct gattattacc gttaccccgc ccgacagtta cgacaccctc
     9361 ctcaaaacct gccgtaaaaa gcagctccga cacatcatcc tcatacagga ctgggacagc
     9421 ctgtctgccg cagaactgca caccgccgaa actgccatcc gcaaacacca cggcaacgga
     9481 ctcaacatca ccgcctacac caccgcaggc atccaactgc cctcactcgg actcaacatc
     9541 agtacccaag acggatatgc cgcaggccat accgccatac tgaccggcaa tgccgccgtc
     9601 agccgccaaa tcgacaacat cctgaacaaa ctccgtcaag gcacatcccg ccacatcaac
     9661 ctgcatcccg gcatcagccc catcacatcc gccgattggc tcaaccgctt cggacacagc
     9721 ctgcacacca aaaccgccgt cctacaccac aaccctgaag aggatcagcg caggctgttc
     9781 agcgcaatcc gccaatttac ccgccatacg ccgctgattc tccacatcac ctgcctcacg
     9841 acagaaaccg accgtgccgt actgcactgc ctcgcccgac actgcaactt cctcatcagt
     9901 ttcaacgccg acgacctcga agccgcactg agcgcccaac tgtccgacct tccacccctg
     9961 tcccgactcg acatcctgtc cgacacgcct gccgaatggc tgcacgcgca cgcaccaaaa
    10021 aacctcaccc tccactttcc caaccttccc caccacatcc gcaacggaca cctgaccggc
    10081 acacccacac cctcaatctg ccacgacatc gcctcacgtc agcttgcccg ccccgacacc
    10141 caagccgtcc tgaccatcct cggtccctcc ggacacgagg attacaaaaa aacagcacgc
    10201 gcccttatcc gcctgtccga acagaccgcc aaacccctgc tcgtcaacag ccccttttca
    10261 gacggcataa cacatttcga cacccccgca caggcaatcc gcaccctttc ctaccgtaac
    10321 accgccgccg ccctgaaaca ggcacagctc gacattgcac cgccgcagcc atgccgtctg
    10381 aaaacaccgc aaccccaaaa catcaaaaaa gcccttgcag cggcaaatcc cgccctgctc
    10441 gccgaagccc tgcacctccc cctaccggca caccacccat aacgccgtcc aattccaatt
    10501 cggcagccac cccctctatg gcgacatcct gaccgcacgc tgcaacggac aaaccactgc
    10561 cgtactcccg ccgtttacca cgctcgacag ccgccacctt gcccgctttg ccgaactcga
    10621 cggcacacaa accctcgatc agttcctgca cacactgacc gtcatccccg aataccgcca
    10681 acacattttc ggcatcaccc tcaacctcaa cggcggacaa tacagcagcg atttcatctt
    10741 aaagacaccc ggaacacacg acacgcccaa acgcaagaac acaggcaaag ccgcccaaac
    10801 cctcgaacat gccgccgcaa aaatgcagag tgccgccgca tacctggaac acaaaaaccc
    10861 gacagccgcc gaatttctcc gccacacaag cgaagccgcc gcagaactgc tcggcagcaa
    10921 aaccgaaacc ggagcagccg tacccaacgt acttgccccc tatcccgcag cacaccccaa
    10981 aacactgtcc ctaaaaaaca acacgaccgt taccattatc ccccttttac ccgaagacgc
    11041 agaagccaaa cagcagttcg tccgcagcct cggccccgaa gcacggtaca cacgcttcat
    11101 gacccacacc aacgaactgc ccgcagccac gttggcacgc ctgtgcaacc ccgattacca
    11161 ctgtgaaccg catggacggc aagggatgcc gacagcaaca tcgtcgccgt cgtccgccac
    11221 agccgcctga accgcaacga atgcgaattt ggcatcacac tggcggaaca tatgcgcggc
    11281 agcgggctgg cacagaaaat gatggaactc atcatccaaa ccgccgcaca gcaaggctac
    11341 ctgagcatga gtgccgacat tctcaaaacc aataccccca tgatcaaact tgctgaaaaa
    11401 tcaggattta ccctcaagga atcggacacc gaaaaaaacc tgtaccgcgc atatctgaac
    11461 cttgcggcag acaaaacaac aggaaaaaca aataaaaact tgcgcaccgg ccacaaaata
    11521 acctaaaatc ggcagtttcc atatatccga ttttctcaaa aggactcaaa atggtagtta
    11581 tccgtttggc acgcggcggc tcgaaacacc gccccttcta caacgtcatc gttactgact
    11641 cacgcagccg ccgcgacggc cgcttcatcg aacgcgtagg cttctacaac cccgtagcca
    11701 acgaaaaaca agagcgcgtc cgcctcaatg cagaccgcct gaaccactgg attgcacaag
    11761 gcgcgcaagt cagcgactcc gttgcaaaac tgattaaaga acaaaaagcc gtctaatccg
    11821 cgtttgccgc catgacagac actcaaaacc gggtagccat gggctacatc aaaggcgtat
    11881 tcggcataaa aggctggctg aaaattgccg ccaacaccga atattccgac agccttttgg
    11941 actaccccga gtggcatttg gccaaggacg gcaaaaccgt cagcgttacc cttgaagccg
    12001 gaaaagtcgt caacggcgaa ctccaagtca aattcgaagg catagacgac cgcgattcag
    12061 cattctcatt gcgcggttac accatcgaaa taccccgtga agcattcgcc ccgacagaag
    12121 aagacgaata ctactgggca gacttggtcg gcatgaccgt cgtcaacaaa gacgataccg
    12181 ttttaggcaa ggtaagcaac ctgatggaaa ccggcgcaaa cgacgtattg atgattgacg
    12241 gagaacacgg gcagattctg attccgttcg tttcccaata tatcgaaacc gtcgataccg
    12301 gcagcaagac cattactgcc gactggggtt tggactactg atgcttatcc aggcagttac
    12361 cattttcccc gaaatgttcg acagcattac ccgctacggc gtaacgggac gcgcgaacag
    12421 acagggaatc tggcagtttg aagcagtcaa tccccgaaag tttgccgaca acagattggg
    12481 ctatatcgac gaccgcccgt tcggcggcgg tccgggaatg attatgatgg ctccgccgct
    12541 tcatgcggcg atagaacacg ccaaagcaca atcttcccaa accgcaaaag tcatctacct
    12601 cagcccccaa ggaaaaccgc tgacacacca aaaagcggca gaactggcag aacttacgca
    12661 tctgattctg ctgtgcggac gctatgaggg aatagacgaa agactgctgc aaagcagcgt
    12721 cgatgaagaa atcagcatcg gagacttcgt cgtttccggc ggagagcttc ccgccatgat
    12781 gctgatggat gcggtattga ggctcgtacc cggcatattg ggcgacattc agtctgccga
    12841 acaggattcg ttctcaagcg gtattttgga ctgcccccac tacaccaaac ccttagaatt
    12901 tcaaggcatg gctgttccgg aagtattgcg ctccggaaat catggcttga tagcggaatg
    12961 gcggttggaa caatcgctgc gccgcacctt ggagcgcaga cccgatcttt tggaaaagcg
    13021 cgttttaatc ccaaaggaat cccgcctctt gaataaaatc ctacaagagc aacgggaaat
    13081 ccaatcataa tttaggaaaa aacaatgaac ctgattcaac agctcgagca agaagaaatt
    13141 gcccgcctga acaaagaaat ccccgaattc gcaccgggcg acaccgtagt cgtatccgta
    13201 cgcgtcgtgg aaggtacccg cagccgtctg caagcctacg aaggcgtggt tatcgcccgt
    13261 cgcaaccgtg gtttgaacag caacttcatc gtccgcaaaa tctccagcgg cgaaggtgtt
    13321 gaacgtactt tccaactata ttcccctact gttgagaaaa tcgaagtcaa acgccgtggc
    13381 gacgtacgcc gtgccaaact gtactacctg cgcggtctga ccggcaaagc tgcacgcatc
    13441 aaagaaaaac tgcctgcacg caaaggttga ttcaaaccgt tttcccccaa tgccgtctga
    13501 acgttcagac ggcatttttt attgccgcga tgccggcaag gcaacgatat gcctgaccct
    13561 catccacctt aattaacaat cactcccgcc tattcaagca gaatttacag actaaacgtt
    13621 atacagtatc atatccgcca tataacgata accgctttgc caacttccct ttcaaaactg
    13681 caaatcatta tgacaccttc cctcttactg tcaggattga ccttccgact cttcctcgcc
    13741 ctgattgccg tattcctttt atggggtgtt tacctttggg cggtatcggt atgagcatca
    13801 tcgttgaaaa cctgacggtc agctaccgcc gccgccccgc cgtacaccac gtcgacatta
    13861 cttttgaaga acatagtatg tgggcggttt tcggtcccaa cggcgcagga aaatctacct
    13921 ttctcaaatc cttaatggga ttgcagccta tcgatacggg cagcatccgg ctggacggat
    13981 tgaccagtca aaacattgcc tatctccccc aacaatccga catcgaccgt tcccagccta
    14041 tgaccgtttt cgacttggcg gcaatggggc tgtggtatga aatcggtttt ttcaaaggga
    14101 taaataccgc tcaaaaacaa cgcgttcacg aagccttgga gcgtgtcggt atgcggcagt
    14161 ttgcccgccg ccaaatcgcc cacctctcaa acggacaatt ccaacgcgtc cttttcgccc
    14221 ggatgctggt tcaaaatgca aaattcctgc ttttggacga acctttcaat gctgttgatg
    14281 cacggacaac ctacgagctt ctcgacgtgt tgcaaaaatg ccattgcggc ggacacgcta
    14341 tcattgccgt tttgcacgat tacgaacaag tccgtgccta ctttcccaat accctgctgc
    14401 tcgcccgcga aaaaattgcg gcaggcgcaa ccgagaccat tctgacagaa tcctacctcg
    14461 cccaagccag cgccaaaatg cagcaacagg aaagccccga ctggtgcgcc tcataaatac
    14521 tgcccgaaac cacgccccac accagacacc atgaacctct acgacctgct ccttgccccc
    14581 tttgcagaat tcgacttcat gcactacgcc ctcgcatccg tcttctgcct gtccctcagc
    14641 gccgcacccg tcggcgtatt cctcgtcatg cgccgtatga gcctgatagg cgacgcattg
    14701 agccacgccg tcctgcccgg tgccgccgtc ggctacatgt ttgccggctt gagcctgccc
    14761 gctatgggtg tgggcgggtt tgccgccggt atgctgatgg cgctgcttgc cggactcgtc
    14821 agccgcttta ccaccctgaa agaagatgcc aactttgccg ccttttacct gagcagcctc
    14881 gccatcggcg taatcctcat cagcaaaaac ggcagcagcg tcgatttact ccacctcctt
    14941 ttcggatctg tgcttgccgt cgatattccc gcactgcaac tcatcgccgc cgtctccggc
    15001 ctcacgctca ttacccttgc cgtcatctac cgccccctgg tgctagaaag catagacccc
    15061 ctgttcctca agtccgtcaa cggcaaaggc gggctttggc acgtcgtttt cctcatcctc
    15121 gtcgttatga acctcgtatc cggcttccaa gctctcggca ccctgatgtc ggtcggactt
    15181 atgatgctgc ccgccattac cgcccgctta tgggcaagaa atatggggac gctcattctg
    15241 ttgtccgtcc tcatcgccct tttttgcggt ttgatcgggc tgctcatttc ctaccacatc
    15301 gaaatccctt ccggccccgc catcatcctc tgttgcagcg tcctttatct cttttccgtc
    15361 atactcggca aagaaggcgg catcttgccc aaatggttca aaaaccaccg ccaccacacc
    15421 acctgacccc gcaagccgct tgaacaaaca ggcggcttgt tttaccataa tgaatgttat
    15481 gttatatcac agaagaacag ttatgaaaca cctcaaactc acccttatta ccgcattgct
    15541 ggccaccgcc gcaactgccg caccccttcc ggttgtaacc agtttcagca ttttaggcga
    15601 cgtagccaaa caaatcggcg gtgagcgcgt agccgtacaa agcctcgtcg gagccaacca
    15661 agatactcat gcctatcaca tgaccagtgg cgacattaaa aaaatccgca gtgcaaaact
    15721 cgtcctgctc aacggcttgg gacttgaagc cgccgacatc caacgcgccg tcaaacagag
    15781 caaagtatcc tatgccgaag cgaccaaagg catccaaccc ctcaaagccg aagaagaagg
    15841 cggacaccat cacgaccacg atcatgacca cgaaggacac caccacgacc acggcgaata
    15901 tgacccccac gtctggaacg accctgttct tatgtccgac tatgcccaaa acgtcgctga
    15961 agccctgata aaggccgatc ccgaaggcaa agtttattat caacaacgct tgggcaacta
    16021 ccaaatgcag cttaaaaaac tgcacagcga cgcacaagcc gcatttaatg ccgtccctgc
    16081 cgccaaacgc aaagtcctga ccgggcacga cgcattttcc tacatgggca accgctacaa
    16141 catcagcttc atcgccccgc aaggcgtgag cagcgaagcc gagccgtccg ccaaacaagt
    16201 cgccgccatc atccggcaaa tcaaacgcga aggcatcaaa gccgtattta ccgaaaatat
    16261 caaagacacc cgcatggttg accgcatcgc caaagaaacc ggcgtcaacg tcagcggcaa
    16321 actgtattcc gacgcactcg gcaacgcgcc cgcagacacc tacatcggca tgtaccgcca
    16381 caacgtcaaa gccttgacca acgcgatgaa gcaataaaac cgtttgattg gtttgaattt
    16441 gaaaaggcaa aaggccgtct gaaaattaaa tacgaggttt tcagacggcc ttctcttgtg
    16501 caataaagca gtaaacaaat attgataaga aaaaaacaat cagtattgaa aatatcgtta
    16561 aaaatattat ttagacagat tacatggaat ttcattaaca tcagtagact ttgatttcaa
    16621 tcaagaaaac aatgtcatga aaaatattct cttagtattt gttagctttg tgccattatg
    16681 tgtccgcact gatctgccgc tgaatatcga agacataatg accgacaagg gaaaatggaa
    16741 actggaaact tcccttacct acctgaacag cgaaaacagc cgcgccgcac ttgcctcacc
    16801 ggtttacatt cagaccggct ccgcttcctt tatccccgtc ccgaccgaaa ttcaggaaaa
    16861 cggcagcaat accgatatgc tcgccggcac gctcggtttg cgctacggac tgaccggcaa
    16921 taccgacatt tacggcagcg gcagctatct gtggcacgaa gaacgcaaac tcgacggcaa
    16981 cggcaaaacc cgcaacaaac ggatgtccga catatccgcc ggcatcagcc acaccttcct
    17041 taaagacggc aaaaatcccg cactcatcgc tttcctcgaa agcacggttt acgaaaaatc
    17101 gcgcaacaaa gcctcgtcgg gaaaatcgtg gctcatcggc gccaccacct acaaagccat
    17161 agatccgatt gtcctttccc tcaccgccgc ctaccgcatc aacggcagca aaaccctttc
    17221 agacgacgtc aaatacaaag caggcaatta ctggatgctg aatcccaaca tctcatttgc
    17281 cgccaacgac agaatcagcc tgaccggagg catccaatgg ctgggcaaac agcccgaccg
    17341 catagacggc aaaaaagaat ccgcaagaaa cacatccacc tacgcccatt tcggcgcagg
    17401 tttcggtttc accaaaaccg cggctttaaa cgcatccgca cgtttcaacg tttcagggca
    17461 aagcagttcc gaactgaaat tgggcataca gcatacattt taagcagatt ttgattttcc
    17521 aaccttgtaa tccaaaggag ttattatgaa aaaacaaatc accgcagccg taatgatgct
    17581 gtctatgatc gcccccgcaa tggcaaacgg attggacaat caggcatttg aagaccaagt
    17641 gttccacacg cgggcagatg cgccgatgca gttggcggag ctttctcaga aggagatgaa
    17701 ggagactgaa ggggcttttc ttccattggc tatcttgggt ggtgctgcca ttggtatgtg
    17761 gacacagcat ggttttagtt atgcaacgac aggcagacca gcttctgtta gagatgttgc
    17821 tggcggatta ggcgcaattc ctggtgatgt aggtgctgca ggaaaggttg tttcctttgc
    17881 taaatatgga cgtgagatta aaatcggcaa taatatgcgg atagcccctt tcggtaatag
    17941 aacaggtcat cctattggaa aatttcccca ttatcatcgt cgagttacgg ataatacggg
    18001 caagactttg cctggacagg gaattggtcg tcatcgccct tgggaatcaa aatctacgga
    18061 cagatcatgg aaaaaccgct tctaactcct cctttttttc tatttgaaga tgtgtctttg
    18121 gatatatttc aaggattgtc ggaattggaa aagaaaatag agccgcagga tttgatggat
    18181 gatgtttacc gtgcttttga ttcggttgga aatattctga atttccgtat agtagaaaaa
    18241 gagcaaaaag ggttttgggt aagtaccaaa atcaaaacag ttgtatttga ttcggcagat
    18301 atgccttctg atgatctttt tctgaaatgc ttacagtctt catataaggc ttattctgaa
    18361 actgaacctg ccggtttaga taaacggcag ctgatgaaaa cgttaattca aaaatgcgga
    18421 ttcagttgtt gacgaaaaaa tggattctga actaacagaa actgatttcg accatgtatg
    18481 aggtaaaaaa tgtctttcgg ctatttgatt gcaacatcgc aaccttgcga attgttgaca
    18541 aaatctcgag gtgaaacttt ttctttaatt atggataaga tggatttatg gatttatttc
    18601 agattttgtg aaggaaatat ttatacaata aggaagaatg aaactgaatc ttgccttaca
    18661 gaaagaggag gtgaatggtt gaaacatatt tatgaattta atcgggaaag ttttattttt
    18721 tctgatgtgc ttctacaaaa gggagaaagc gaagagaatt tttcagaaat tgtcttaaaa
    18781 tcaataaaaa ataataaaat tttgacagtt gaggtacggt caggcttgca ttttgactta
    18841 agaaatattt atcgtattga gatgtaataa tgcaattgat atgtgccgat tggacaggta
    18901 taggaaattt tatgaaaacc ttcgaaaaaa catggtctgc acaatatcgg gatatggaaa
    18961 tttcagtacg gaatttttgg aatttgaaac ggacaggggc ggaagtctat atcaacggca
    19021 ggcgggttta tcataacgaa gccgggatgg cgtctgcttc tttgcgttcg ctaatggggg
    19081 aatacctgga atttgaagaa agcggtacga aaattaccgt tgaaatcggc agcgcgtggc
    19141 attttaatga aactataaga aatattaata cacatttaag aggtacgaaa cttccaggtt
    19201 ctaactggag agttcaagat aaggggcatt ttcatctttg gaaaagataa taaattctaa
    19261 ggtaagggag actaatttta atcaggcatg gcgtgttata cgcgccatgc aaataaataa
    19321 tatatctgat gaacaggtta tgattgtcaa aatgtcttgt tatcttaaga aagatgaaat
    19381 ttttaagaat atagaaagat atggatatca gtcatcagat attaagaaaa ttagtcctcc
    19441 ttttccatta aatattttac ctgttcccaa accttattat ttttatcttt atgataccca
    19501 tttgaatcaa atttatgatt tggcttggtc atgtaatgat tttttaatgt ttgaaatatt
    19561 tattttggat agggaaagca tcaaatatat agataaagat aaacttttta aacctggaga
    19621 aacactatat ttgaatttta aatctattaa aaatcaatgc aaaaattatt tttttgcggt
    19681 tttggttttg atgcccctta ctttaaaagc ggtgcgagtc aagtggcaga ttataatttt
    19741 gttcctgatg ccttttcttt attctttatt taatcaaatt atttgaataa gcgtagggtg
    19801 ggcacttgct gcccgcgtgt tctgtttcaa gtttattatt gggtgaatat ttggaatttg
    19861 aagaaaacgg tacaaagatt gctgcggaaa tcggcagtgc atggcatttt ttgggcgcgg
    19921 cttgtcggat ttcaataaat ggaaagtatt atgcgggtaa tcggattgtt tggtttgcca
    19981 aaaaagccga aagctaggaa taatttgaca agggcagcct gaaagatttt caggctgcct
    20041 tttatgtgga aaatatgatg aagttcaaat atgtttttct gttggcgtgt gttgccgttt
    20101 cttta
//
LOCUS       DBJGGI010000033        19571 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.033, whole genome shotgun sequence.
ACCESSION   DBJGGI010000033 DBJGGI010000000
VERSION     DBJGGI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19571
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.033"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(149..1219)
                     /gene="lptG"
                     /locus_tag="ACSIGJ_001218"
     CDS             complement(149..1219)
                     /gene="lptG"
                     /locus_tag="ACSIGJ_001218"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HHQ1330083.1"
                     /translation="MNLISRYIIRQMAVMAVCALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLASLSQLAAGSELAVIKASGMSTKKLLLI
                     LSQFGFIFAIAAVALGEWVAPTLSQKAENIKAAAINGKISTGHTGLWLKEKNSIINVR
                     GMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSIMGTDKIETSA
                     AAEETWPIAVRRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTQIYAIAWWRKLVYP
                     VAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGTPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            complement(1216..2331)
                     /gene="lptF"
                     /locus_tag="ACSIGJ_001219"
     CDS             complement(1216..2331)
                     /gene="lptF"
                     /locus_tag="ACSIGJ_001219"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HHQ1330084.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAILIAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGEFNNLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTISTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            2320..2502
                     /locus_tag="ACSIGJ_001220"
     CDS             2320..2502
                     /locus_tag="ACSIGJ_001220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330085.1"
                     /translation="MINHKIAVRGAVSGSAQADCPRKVSDGASKNGVQSCQPSSDGLL
                     SERPSENYRQPSQSGE"
     gene            2505..3911
                     /locus_tag="ACSIGJ_001221"
     CDS             2505..3911
                     /locus_tag="ACSIGJ_001221"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HHQ1330086.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCADKAPEHNTAAHALFSALEEGQ
                     NFSDTKIPTDNGLQAVAVVRLEKTDRAALNKAAAEAAKWAQNQETVNVDVHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFSQAVFHSAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAIVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLMNYLRNL"
     gene            3974..4414
                     /locus_tag="ACSIGJ_001222"
     CDS             3974..4414
                     /locus_tag="ACSIGJ_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HHQ1330087.1"
                     /translation="MPKVTFYTHVDQIPLFTCRLIARAIRDGGRILVWSDSFGRLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSDTSVLLACGGNLPRIPEGMAVLNLSDGFWNTA
                     SVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            4674..5492
                     /locus_tag="ACSIGJ_001223"
     CDS             4674..5492
                     /locus_tag="ACSIGJ_001223"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HHQ1330088.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASAAQGDT
                     SSIGGTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTDAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGK
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPATFPLSQVIPGWTEGVRLLKEGGEA
                     TFYIPSNLAYREQGAGEKIGPNATLVFDVKLVKIGAPENAPAKQPDQVDIKKVN"
     gene            5599..6225
                     /gene="purN"
                     /locus_tag="ACSIGJ_001224"
     CDS             5599..6225
                     /gene="purN"
                     /locus_tag="ACSIGJ_001224"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HHQ1330089.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AERGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGQIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            6241..6912
                     /locus_tag="ACSIGJ_001225"
     CDS             6241..6912
                     /locus_tag="ACSIGJ_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HHQ1330090.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAARLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPAYYKDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVTYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QAAKP"
     gene            7030..7452
                     /locus_tag="ACSIGJ_001226"
     CDS             7030..7452
                     /locus_tag="ACSIGJ_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HHQ1330091.1"
                     /translation="MQVTSKWIDGMCFVGTAEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVIGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGAEK"
     gene            7975..8691
                     /locus_tag="ACSIGJ_001227"
     CDS             7975..8691
                     /locus_tag="ACSIGJ_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHQ1330092.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILAGLPDLETRLKPLYAMIRCEEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREKKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            8840..9742
                     /locus_tag="ACSIGJ_001228"
     CDS             8840..9742
                     /locus_tag="ACSIGJ_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HHQ1330093.1"
                     /translation="MSSLMTLFSVLVPMFAGFFIRVPKPYLPASDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLGDMALTVLWLFVCTVGANLLALAVLGKLSPWRIGGKGKGVSVG
                     VSGSVRQLGCVLIGFVSGKLMCDIWMPSENAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     LNRRGIRLSVWFILSSLSGGLLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSTLG"
     gene            9785..10559
                     /locus_tag="ACSIGJ_001229"
                     /pseudo
     CDS             9785..10559
                     /locus_tag="ACSIGJ_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
     gene            10671..12359
                     /locus_tag="ACSIGJ_001230"
     CDS             10671..12359
                     /locus_tag="ACSIGJ_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HHQ1330094.1"
                     /translation="MLNKDQFADNHFIRTIIEDDLKSGKHEAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     DYFDRLYDYAVGLIKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMAAGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPALHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGVRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFQAGKTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCGEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRQDHGKDMPVFNRTVTLKDSWQPK"
     gene            12451..13407
                     /gene="gshB"
                     /locus_tag="ACSIGJ_001231"
     CDS             12451..13407
                     /gene="gshB"
                     /locus_tag="ACSIGJ_001231"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HHQ1330095.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     NGGLVTAQASAFEFSGAKNDDDHEWFKAADKVQTALKAFDAVIMRTDPPFDMQYLYST
                     QLLTLAEQQGAKVFNSGRAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IVKPLDGMGGMGIFRLTEKDPNIGSILETLMRFDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRSGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKSFDVAAMFADAVAAWSVR"
     gene            13566..13949
                     /locus_tag="ACSIGJ_001232"
     CDS             13566..13949
                     /locus_tag="ACSIGJ_001232"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HHQ1330096.1"
                     /translation="MGPSSYAAEKKGKSGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNALLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLVAMLMLAAVWLSALFG"
     gene            14081..14554
                     /locus_tag="ACSIGJ_001233"
     CDS             14081..14554
                     /locus_tag="ACSIGJ_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HHQ1330097.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKMGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKTAPMPAMNHGHHHGEAHQH"
     gene            14679..15782
                     /gene="mnmA"
                     /locus_tag="ACSIGJ_001234"
     CDS             14679..15782
                     /gene="mnmA"
                     /locus_tag="ACSIGJ_001234"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HHQ1330098.1"
                     /translation="MNTTSNTSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWEN
                     DDNDEYCSIKQDSFDAIAVADIVGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTIGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELCYLDDETAELVFDEPQW
                     AVTPGQSAVLYDVDICLGGGIIQTTDKPVIITR"
     gene            15853..17523
                     /locus_tag="ACSIGJ_001235"
     CDS             15853..17523
                     /locus_tag="ACSIGJ_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HHQ1330099.1"
                     /translation="MEKIWLESYEKGVSAEIDITQYNSVSDVFRQSVEKFARLPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVARGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLIANPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVPSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDACGFLETGDIAVMDEKGRLK
                     LVDRKKDLVVVSGFNVYPNEIEEFIAHHEKVMEVACIGVPNEKTGEALKVFVVKKDPS
                     LTKEELTAFCRTGLTAYKVPKDIEFRDELPKSNVGKILRRELRQSAGK"
     gene            17635..19269
                     /locus_tag="ACSIGJ_001236"
     CDS             17635..19269
                     /locus_tag="ACSIGJ_001236"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HHQ1330100.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRKMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAVKNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NKGDVSVLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAVKIYGSGHIRERHRHRYEVNNNYVSALEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
     gene            19411..>19571
                     /locus_tag="ACSIGJ_001237"
     CDS             19411..>19571
                     /locus_tag="ACSIGJ_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HHQ1330101.1"
                     /translation="MKITHCKLKKEVQKEPLRSFVPEVTARSAADILGIHPDSAALFY
                     RKIRTVANHR"
BASE COUNT         4811 a         5244 c         5292 g         4224 t
ORIGIN      
        1 gccgcagaca gtacaaatag tacggcaagg cgaggcaacg ccgtaccggt ttaaatttaa
       61 tccactataa tagggaacgg ctatatgtct gcgggaatgt gtcaatgaaa acaaatgccg
      121 tctgaagaga ggttcggacg gcatttgttc aacgtttttc ctgtttgcgt atcagccaaa
      181 cagcgagcaa ggcgaaggct atggtaggca gtgcgccggc gaggaagggt ggggtgccgt
      241 agagttggct ggtaaacccg aagagcctgc cggcaaggtg gaacagcaat ccgagacaga
      301 tgccgccgaa gagttttaag cccatattgc cgtggcgcgt ggtttgcggc gtaaaggcga
      361 aggcaacgag cgccatgacc catgcggcga cggggtaaac gagtttacgc caccatgcga
      421 tggcgtagat ttgggtgttt tggctgttgt tttggaggtg gcggatgtag gtggtcagct
      481 cgccgacgga catttggtcg ggcttgacga gcaatacgtc catcaggttg cgtctgacgg
      541 caatcggcca agtttcttcg gcggcggcgg atgtttcgat tttgtctgta cccatgatgc
      601 tgcggcggat gtttttcaac tgccagctgc cgtcgctgtt caaaacggcg gaatcggctt
      661 ccactgcctc tgccaattcg tttttatcgt tgcgcgccca aattttgatg cccaaaagcg
      721 tatggtcggg caacattccg cgcacattga taatgctgtt tttttctttc agccaaaggc
      781 cggtatggcc ggtgctgatt ttgccgttaa tggcggcggc tttgatgttt tcggcttttt
      841 ggctcagcgt gggcgcaacc cattcgccga gcgcgacggc ggcaatagca aaaataaaac
      901 cgaactgaga cagaatcaac agcagctttt tggtgctcat gccgctggct ttgatgacgg
      961 ccagttcgct gccggcggca agctggctga gagaggccag tccgccgatg aggacggcga
     1021 ggggcatgag ttcgtaggcg cgggcgggca ttttgagggc ggtgtagccc agcatttccc
     1081 atatgccgta actgcctttg ccgaggttgc cggtttcgta caggatttca aaaaagctgt
     1141 acaaagcgag gaaggcaagg agcgcgcaaa ccgccataac cgccatttgg cggatgatgt
     1201 aacgtgaaat caggttcatt ttccgccttt caatgtcaga cttttgccaa ccgcctgcca
     1261 gaagggctgg ctgggcatac tgcggacgcg cagaagtacg attgcgatga cgaacatgat
     1321 gatgtgcata ggcagcagtc cgagccaaaa atggattttg ccgtcttcca cggcttcaaa
     1381 aagcagggtc agcccgtttt ggtaaattaa aaacaaaccg atggcaatca agatattgta
     1441 ggtatgtccg ctgcgcgggt tgaaatagga aagcggcacg gcgagtaggc agagcaggag
     1501 gacgctgacg gtcagcgaga tgcgccacat caattctgcc tgatgttgcg gattgctgct
     1561 gccaatcagt tgggcggtcg aaatggtgcg gcggtgggaa acggggtcga taagtttggg
     1621 cgtggtgctg ataatcaggt tgagtttttg gaaggaaacc tgattgtagt cggcgcgtcc
     1681 gggcgtgccg ctgtaacggt agccgtggcg caattcgagc gtgcgtttgt tgtcgttcag
     1741 cgagaagtta ccttctttgg cgaagatgat gttgtcgccg ccgtttttgt cctgttcgcg
     1801 caggaacagg tttttcatga tgccggattc ggtgtcaaag gtttcgacga aataaaccct
     1861 gccgttgcgc ttgcccaagt tattgaactc gccggcttcc accaaagaca attcctgctt
     1921 ctgcttcaaa atttcggcat attcgcggct gcgcagttcc gcccacggta tcacccaaag
     1981 ctgcatgacg gcaatcagga tggcaaacgg cacggcaaac tgcatgacgg ggcgtatcca
     2041 ctgtttcaac gccaatccgc aggatagcca gaccgacatt tcgctgtcgc gccagtagcg
     2101 ggtcaatacg gtcagcgtgc tgatgaatgc ggtcaacacc agcaaaagcg gggtcatacc
     2161 gatgacccag aagccgacta aggccaacac ggcatcgatg gcgacacgcc cgtcagctgc
     2221 gcggccaagc aggttgatcg cctgcgtgga caccaacacc gccaagagga cgacgaaaat
     2281 gccgacggcg gtaaaagaga gttctttgat gaggtttctt tgataaatca taaaatcgcg
     2341 gtcagggggg cggtaagcgg ttcggcgcag gcggattgtc cgcgcaaggt ttcagacggt
     2401 gcttcaaaaa atggggtaca atcgtgccag ccgtcttcag acggtctttt gagcgaaagg
     2461 ccgtctgaaa actaccgaca accgtctcaa tcaggagaat aaacgtggaa tttagcacaa
     2521 aaaccgaaat cttgcaggaa cagcaggcag gcgcgcagtt atttgtctgt gccgacaaag
     2581 cacccgagca caacaccgcc gcacatgcgc tcttctctgc tttggaagag ggtcagaatt
     2641 tttccgacac caaaatcccg acggacaacg gtttgcaggc agtcgccgtc gtccgcctcg
     2701 aaaaaaccga ccgcgccgcg ctgaacaaag ccgccgccga agccgccaaa tgggcgcaaa
     2761 atcaagaaac ggtcaatgtg gacgttcacg cctttgatga ggcgcaagca gcagccgttg
     2821 ccgaagcttt tgccatcgcg ttcggcaacg ctgcatatcg tttcgaccgc tacaaaaaag
     2881 aagccaagcc cgccaaattt tcgcaagccg tgttccacag cgcacacgaa gccgccgtca
     2941 aagaagccct gcgcgttgcc gaagcacaag tttacggaca aagcctctgc cgcgacttag
     3001 gcaatgccgc accgaacgaa tgcacgcctg agttcctcgc gcgtaccgcc aaagccgaag
     3061 ccgagaaact gggcgcgcac gccaaaatca tcgaaaaaga ctacatcaaa gaaaacatgg
     3121 gttcgttttg gtcggttgcc aaaggcagcg tcgaagaccc atatttggtt gaactgagct
     3181 atttcggtgc ggccgacaaa gaagccgcgc ctgtggtatt ggtcggcaaa ggcattacct
     3241 tcgacaccgg cggcatttcc ctcaaacccg gcctgaacat ggacgaaatg aagtttgaca
     3301 tgtgcggcgc ggcaaccgtc atcagcacct tctgcgccgc cgtcaaactg caactgccga
     3361 tcaatctgat tgccatcgtc gccacttgtg aaaacatgcc ttccggcgcg gcaaacaaac
     3421 cgggcgatgt tgtgaaaagt atgaaagggc tgacgattga agtgttgaac accgatgccg
     3481 aaggccgtct gattttgtgc gacgcgctca cttacgccga gcagttcaaa cccaaagccg
     3541 tcatcgatgt cgccaccctg accggcgcgt gcatcgtcgc cttggggcac gatgtcagcg
     3601 gcgtgatggg caataatcag gatttgatcg acagcctgct tgccgcttcc tacaacgtgg
     3661 acgacaaagc gtggcaactg ccgctctttg aaacctacaa agaccagctc aaatccaact
     3721 ttgccgacat ccccaatatc ggcacgcccg gcgcgggcac gattaccgcc gcaacattcc
     3781 tgtcctattt caccgaaggc tacccgtggg cgcaccttga catcgcgggt acggcgtgga
     3841 aatccggagc ggaaaaaggt gcgaccggcc gccccgtacc gctgctgatg aactatctgc
     3901 ggaatcttta attttgccac aatgccgtct gaagcttttc agacggcatc atcctgtcaa
     3961 atgctcaaac aatatgccga aagttacctt ttatacccat gttgaccaaa tccccctttt
     4021 tacctgtcgg ttgattgcgc gcgctatccg agacggcggc aggatactgg tgtggtccga
     4081 ctcgttcggg cggcttcagg aattggacaa aatgctttgg caatacgagg ccgagagttt
     4141 tattccgcac gaaatttggg aaacggaaga agccatgccg tctgacacat ccgtcctgct
     4201 tgcctgcggc ggcaatctgc cccgaattcc cgaaggcatg gccgttttga acctgtccga
     4261 cggtttttgg aacaccgctt cggtcttgcc cgcgcgcgtt ttggaaatcg tcggcaacag
     4321 cctggaagat ctcgccgacg cgcgcgaacg ctttaccgcc taccgccgaa gcggttttgc
     4381 catcgaacat cacggcatgg agggcaaggc atgaaatgcc gtctgaaatg ccgcatttac
     4441 aaaatttaaa caggcttgat tctatttgat taaaatttcc ttatcggttg aaccccgcca
     4501 cttggacatc tgtccttcgg ggcggtagaa tcaaacctta ttttggaagg ttcaatccct
     4561 tccaaaacag ggcaacacac agattgacgc tttatgtgcc atcctgtgtg ttgaaacatt
     4621 caaactcggc tataatcccg tttttccgac tttatcgaca gcgaagatcc atcatgaaca
     4681 ccattttcaa aatcagcgca ctgacccttt ccgccgcttt ggcgctttcc gcctgcggca
     4741 aaaaagaagc cgcccccgca tctgcatccg aacctgccgc cgcttctgcc gcgcagggcg
     4801 acacctcttc aatcggcggc acgatgcagc aggcaagcta tgcgatgggc gtggacatcg
     4861 gacgctccct gaaacaaatg aaggaacagg gcgcggaaat cgatttgaaa gtctttaccg
     4921 atgccatgca ggcagtgtat gacggcaaag aaatcaaaat gaccgaagag caggcccagg
     4981 aagtgatgat gaaattcctg caggagcagc aggctaaagc cgtagaaaaa cacaaggcgg
     5041 atgcgaaggc caacaaagaa aaaggcgaag ccttcctgaa ggaaaatgcc gccaaagacg
     5101 gcgtgaagac cactgcttcc ggtctgcagt acaaaatcac caaacagggt aaaggcaaac
     5161 agccgacaaa agacgacatc gttaccgtgg aatacgaagg ccgcctgatt gacggtaccg
     5221 tattcgacag cagcaaagcc aacggcggcc cggccacctt ccctttgagc caagtgattc
     5281 cgggttggac cgaaggcgta cggcttctga aagaaggcgg cgaagccacg ttctacatcc
     5341 cgtccaacct tgcctaccgc gaacagggtg cgggcgaaaa aatcggtccg aacgccactt
     5401 tggtatttga cgtgaaactg gtcaaaatcg gcgcacccga aaacgcgccc gccaagcagc
     5461 cggatcaagt cgacatcaaa aaagtaaatt aagtccgaat ccatgcccga aacaggtttt
     5521 cgggcatttt tacggcaaat gccgtctgaa gccaccaaac agcggttcag acggcatccc
     5581 tttcaaaagc gcaccattat gaaaaacatc gtcatcctga tttccggacg cggcagcaat
     5641 atgcaggcaa tcgtcaatgc cgccattccc aacgtccgca ttgccgccgt gttgagcaac
     5701 agcgaaacgg ctgccggttt gcaatgggcg gccgaacgcg gcatcccgac cgacagcctg
     5761 aatcataaaa actttgaatc acgacttgcc ttcgatacgg ccatgatgga aaaaatcgac
     5821 gcatatcaac ccgatttggt ggttttggca ggttttatgc ggattctgac ccctgagttc
     5881 tgcgcccatt acgaaggcag gctgatgaac atccatccgt ccatcctgcc ctcgttcacc
     5941 gggcttcata cgcacgaacg cgctttggaa gcaggttgcc gcgttgccgg ctgtacgatt
     6001 catttcgtta cggccgagct ggattgcggc cagattgtat cgcaagggat tgtgccgata
     6061 cttgacggcg atacggcaga cgatgttgcc gcccgggttt tggctgtcga acacaaactc
     6121 tatccgaaag ccgttgccga ttttgccgcc ggccgcctga ttatcgaggg aaaccgtgtc
     6181 agaaattcgg aaaacgccga tgccgcccgt ttcctgacgg cgtaaaccgg gcgggagcaa
     6241 atgatgaaga cttttaaaaa tatattttcc gccgccattt tgtccgccgc cctgccgtgc
     6301 gcgtatgcgg caaggctacc ccaatccgcc gtgctgcact attccggcag ctacggcatt
     6361 cccgccacga tgacatttga acgcagcggc aatgcttaca aaatcgtttc gacgattaaa
     6421 gtgccgctat acaatatccg tttcgagtcc ggcggtacgg ttgtcggcaa taccctgcac
     6481 cctgcctact ataaagacat acgcaggggc aaactgtatg cggaagccaa attcgccgac
     6541 ggcagcgtaa cctacggcaa agcgggcgag agcaaaaccg agcaaagccc caaggctatg
     6601 gatttgttca cgcttgcctg gcagttggcg gcaaatgacg cgaaactccc cccgggtctg
     6661 aaaatcacca acggcaaaaa actttattcc gtcggcggcc tgaataaggc gggtacggga
     6721 aaatacagca taggcggcgt ggaaaccgaa gtcgtcaaat atcgggtgcg gcgcggcgac
     6781 gatacggtaa cgtatttctt cgcaccgtcc ctgaacaata ttccggcaca aatcggctat
     6841 accgacgacg gcaaaaccta tacgctgaag ctcaaatcgg tgcagatcaa cggacaggcc
     6901 gccaaaccgt aaatcggttt tcaggcggtt ttttgccgtt ttgtccatat ttctgtataa
     6961 gccgcttgaa attgtttaca ataacgccat atgcaaagcg gttttaacgc tattttcggg
     7021 aatgacacca tgcaggttac atcaaaatgg atagacggaa tgtgttttgt cggcacggcg
     7081 gaaggcgggc acagcgtcgt tatggaggga tcggcggcag aaggcgcggc caagcgcggg
     7141 cccagccctt tggaaatgct gctgttgggg gtggcgggct gttcgagcat cgatgtggtg
     7201 atgattgccg aaaaacagcg tcagaaagtg actgactgcc gtgcgacggt tacggcgaaa
     7261 cgggcggacg atgcgccgcg cgtgtttacc gaaatccaca tccatttcaa ggtaatcggg
     7321 catgatttga aagaatcggc cattgagcgc gccgttcaga tgtctgccga aaaatactgt
     7381 tcggcttcga ttatgttggg caaagcggca aagattaccc acagttttga aattgccggg
     7441 gcggagaaat aggcatcggg cggcattatg gggaaacggc tgatgccgtc tgaaggggaa
     7501 aggtgcggca ggcaatccgg aggcgcataa aaaagcagat atattcggac tgcacctccc
     7561 gaatatatct gcctgctgtt tcctctttat tcagccttta taatacttgg acttgtcggg
     7621 gtattgtgca ggcttgattc cggattgtca acaattttcg gtcaaatttt aaatgccgcg
     7681 ttttaaaatg atgcccgcct gattttgcgg gcgggcggaa ggcgggaatc ccgttatttt
     7741 aagccatata ttaatttatt gaattaaaat aaatttgtgc aatatagtgg attaacaaaa
     7801 accagtacgg cattacctcg ccttgccgta ctatttttgt actgtctgcg gcttcgtcgc
     7861 cttgtactga tttaaattta atccactata ctttaatttc tcctacacga tttgaaacac
     7921 agccgtctta aaacatctta aaaaatttga aagcgtaaaa atacggaaaa catcatgaac
     7981 tttgaaaacg acgacattat ccatgcgccg accacgtctt ccctgattct cgaagagcgg
     8041 cacgattcgg agctgttccg tgtttacgcc ctgattttgg acggcattac cgatcaggtg
     8101 ctgctgcccg gcaaaaagct gaccgaatcc gaactttgcc gtcagatggt gtgttcgcgc
     8161 aacaccgtcc gcggcgcgct gtcgcttttg gcgcacgaca agattgtcga tttgcaaccc
     8221 aacaggggcg cgttcgtcca cgttcccgat ttgaaagaaa tgcaggatgt gttcaatgcg
     8281 cgcatcgaaa tggagacgat gattttgaac atcctcgccg gcctgccgga tttggaaacg
     8341 cgcctcaagc cgctttatgc gatgataagg tgcgaagaag aggcctccgg caggggcgac
     8401 cgcgtcggct ggaaccgcct gtccaatgcc ttccacgtcg aactggcgcg cctggtgggc
     8461 aacgatgtgc tgttcgacat tatgaacacg ctgtgcgcgc ggtcttccct gattgtcgcc
     8521 gtggcgggcg tgcatcgcga gaaaaaacac gccatcaata cgcatacgca ttccgaacac
     8581 cgcgaaatcc tcgacctgct gctggcgggc aggcgcaacc gcgtggtcaa aatcctgcgc
     8641 cgccatttgg gcaactgtat ggagcgtttg gaaaagactt tggaagattg aatgccggag
     8701 cggataagcc gcctgaacct tcaggcggtt ttttaatggc gaacctgatg ccgtctgaaa
     8761 tatggatgcg ggtatctgca attttcagac ggcaattttt aagccgcaca tatcatgcgg
     8821 caataaagga gggtagggga tgagcagcct gatgacgttg ttttcggtat tggtaccgat
     8881 gtttgccgga ttttttatcc gtgttcccaa gccttacctg cccgcttcgg acaaggtgct
     8941 gtcggttttg gtgtatgccg tgctgctgct gatcggcgta tcgttgtcgc gcgtggagga
     9001 tttgggttcg cggttgggcg atatggcgtt gacggttctg tggctgtttg tttgtacggt
     9061 aggggcgaac ctgcttgcct tggcagtgtt gggaaagttg tccccgtggc ggataggggg
     9121 aaaagggaag ggcgtttcgg tcggcgtgtc gggcagtgtg aggcagctcg gatgcgtact
     9181 gatcggtttt gtgtccggca aattgatgtg cgatatttgg atgccgtctg aaaacgcggg
     9241 tatgtactgc ctgatgctgc tggtgttcct catcggcgta cagctcaaaa gtagcggcgt
     9301 atcgttgcgg caggttttgc ttaaccggcg gggcatccgg ctgtcggttt ggtttatatt
     9361 gtcatctctt tcaggcgggc tgctgtttgc cgcatcggca gatggtgtgt cgtggacgaa
     9421 aggtttggcg atggcttccg gcttcggttg gtattccctc tcgggtttgg taatgaccga
     9481 ggcttacggg gcggtatggg gcagcatcat gctgctgaac gatttggcac gagagctgtt
     9541 tgcactggca tttattccgc tgctgatgaa gcgttttccg gatgcggcgg tgggggtcgg
     9601 cggcgcgacc agtatggatt tcacattgcc cgtaattcag ggtgcgggcg gtttggaagt
     9661 cgtgccggta gcggtcagct tcggcgtggt ggtcaatatc gccgccccgt ttctgatggt
     9721 ggtgttttcc acgctgggct gaacgcggta aaatcggcat cccgatgcaa ggaagcagaa
     9781 aacgatgaaa ccgaaaatcc aaaggcatgg agagatttta agccttgtcc gccggcatca
     9841 gtttatgtcg gtggacgagc ttgccgccgc attggacgtt accccgcaga cgatacgttg
     9901 cgacatccgc gagttggagg agggcggcag cctgaaacgc catcacggcg gcgcatcttc
     9961 gggcggaaac ttgccggagg gtctgcccgc cgaccgccaa acccggtgtc aaaacgaaaa
    10021 aaacgccatt gcccggctga ttgcggaaca catccccgac ggttcgtcgc tgttcgtcag
    10081 tatcggtacg accatggaag ccgcggcatc agagctggtg aagcggcgca gcagcctgcg
    10141 gattattacc aacaatatcc acgtcgcctc cgtcgtttcg gcacgtacgg attacacggt
    10201 catcatcaca tccggcgtcg tccgcccttt ggacggcggt attaccggcg tggcgaccgt
    10261 cgactttatc aaccagttca aagtcgatta tgccgtgatg agcacgcacg gcgtggaaag
    10321 cgacggttcg cttttggatt acgattacaa ggaagtcagc gtcatgcagg cgatgattgc
    10381 caacgcgcgc gtccgttttc tcggcgtgga tcacagcaaa ttccgcagca acgcgctggt
    10441 caggctcggc ggcattaccg gcgtttgaca aagtatttac cgacaggttg cctgataccg
    10501 cgatgcagaa gatgctgaaa gaggcggggg tggaatgcct gattgccgat gccgtctgaa
    10561 cgctatgtca aagcgcgcaa gtcgggtaca ataaacacat catcaaaccg cttcagacgg
    10621 catacggaac ctccccaatg ccgtctgaag ccatctgttt aaagaaaacc atgctcaata
    10681 aagaccaatt cgcggacaac catttcatcc gcaccatcat cgaagacgac ctcaaaagcg
    10741 gcaaacacga agccgtccaa acccgttttc cgcccgaacc caacggctat ttgcatatcg
    10801 gacacgccaa atccatctgc ctgaacttcg gtttggcgta tatttacgac ggtttgtgca
    10861 acctgcgttt cgacgacacc aacccggaaa aagaaaacga cgaatacgtc aacgccatca
    10921 aagaagacgt cgagtggctc ggtttccatt gggcgggcga gccgcgtttc gcttccgact
    10981 atttcgaccg gctttatgac tacgccgtcg gtttgattaa agacggaaaa gcgtatgtcg
    11041 atgatttgac acccgaagaa atgcgagaat accgcggtac gctgaccgaa gcgggtaaaa
    11101 acagccctta ccgcgaccgc agtatcgaag aaaacctcga cctgttcacg cgtatgaaaa
    11161 acggcgagtt ccccgacggc agcaaaaccc tgcgcctgaa aatcgacatg gcggcaggca
    11221 acatcaatat gcgcgatccc gtcatctacc gcatccgccg cgcccatcac cacaacaccg
    11281 gcgacaaatg gtgcatctac ccgatgtacg attacacgca ttgcatttcc gatgccatcg
    11341 aaggcatcac gcattccttg tgcacgctcg aatttgaagc gcaccgtccg ctttacgact
    11401 gggtgttgga caacatcccc gcgctgcacg ccacccgtcc gcgccaatac gagttttccc
    11461 gtttggagct tttgtacacc attacctcca aacggaaatt gaatcagttg gttgtggaaa
    11521 aacacgtttc cggctgggat gatccgcgta tgccgaccat ttccggtatg cgccgccgcg
    11581 gctacacgcc cgaaggggtg cgcctgtttg ccaaacgcgc cggtatttcc aaatctgaaa
    11641 acatcgtcga catgagcgtg ttggagggtg cgattcgcga agagctggaa aactccgccc
    11701 cgcgcctgat ggcggttttg aacccgctca aagtgaccct gaccaacttt caagccggca
    11761 aaacccaaag ccgccgtgcc gcgttccatc cgaaccacga agaaatgggc gatcgcgaag
    11821 tacctgtttc acaaaccatc tacatcgaag ccgacgactt tgccgaaaat ccgcccaaag
    11881 gcttcaagcg tctgattccc ggcggcgaag tacgcttgcg ccacggctat gtcatcaagt
    11941 gcggtgaagt cgtcaaagac gaagcaggca atgtggttga actcaaatgc agcatcgacc
    12001 acgacacctt gggcaaaaat ccagaaggcc gcaaagttaa aggcgtaatt cactgggttt
    12061 ccgccgaaca cgccgccgaa atcaaagtcc gtctgtacga ccgcctcttt accgtcgagc
    12121 gtcccgatgc cgtgcgcggc gaagacggcg aatacctgcc gtttaccgat ttcctcaatc
    12181 cggaatccgt taaggaaatc actgcttacg ccgaacctgc cgcgaaagat ttgccggcgg
    12241 aaagccgttg gcagttcgag cgcatcggct attttgtgac cgaccgccaa gaccacggca
    12301 aagacatgcc ggtgtttaac cgcacggtga cgttgaaaga ttcttggcag cctaagtaaa
    12361 ccccatcctt gccgtctgaa tattgttcgg gcggcatttc tcctttaccc gcgaaatgcg
    12421 gcacactcgg cacaccggaa aggaaatatg atgaaagtcc tctttatcgc cgacccgatg
    12481 gcaagtttca aaacctacaa agacaccacc tacgcgatga tgcgggaaat ggcaaaacgc
    12541 ggctggcggc tgtttcatac cttgagcggg gaattgtctg taaacggcgg tttggtaacg
    12601 gcacaggcat cggcatttga attttcgggt gcaaaaaacg atgatgacca tgaatggttt
    12661 aaagcggcgg acaaagttca gacggcatta aaagcatttg atgccgtgat tatgcgtacc
    12721 gatccgccgt tcgatatgca atacctttac tccacccaat tactgacgct ggcggaacag
    12781 cagggcgcga aagtgttcaa cagcggacgg gcgatgcgcg actttaacga aaaactggcg
    12841 attttgaatt tcagccgctt taccgcgccc acgctggtaa cgacccgttc cgccgatgtc
    12901 cgcgcatttt tgaaagaaca cggcgacatc atcgtcaaac cgctcgacgg catgggcggc
    12961 atgggcatct tccgcctgac cgaaaaagac cccaacatcg gcagcatcct cgaaaccctg
    13021 atgcggtttg attcccgcac cattatggcg caacgctaca ttcccgaaat cgtacacggt
    13081 gacaaacgca tcttgattat cggcggcgaa gtcgtcccct atgctttggc gcgtatcccg
    13141 caaaacggcg aaacacgcgg caatctggcg gcaggcgggc gcggtgtggc gcaggaattg
    13201 gacggacgcg accgggaaat tgcagagact ctggctcccg agcttaaacg gagcggcatc
    13261 ctgctggccg gtttggacgt tatcggcagc aacctgaccg aagtcaacgt aaccagcccg
    13321 accggattcc aagaaattat gaaacaaaaa agtttcgacg tggcggcaat gtttgccgat
    13381 gccgttgccg cgtggtcggt acgttaaacc gatgccgtct gaaaggcttt tgcttcgtaa
    13441 ccgcttggtt tgttcgggca ggcagggttt tccctcagcc ggcagggatg cgcttaatgc
    13501 cgtacggaca cccctccgcc ccgttttcag acggcatata ttgaggacat tttgaaagga
    13561 taccgatggg accttcctcc tacgcggcag aaaaaaaagg caaaagcggc atcaggcgcg
    13621 tcatcaacgc attcggctat tcgatagacg gcatcgccgc cgcctaccgt tacgaagcgg
    13681 cattccgtca ggttttgtgg ctgaacgcgc tgctggtgtg cgcggcattt ttttgggttt
    13741 ccgaaaccgc cgtccgcctg ccgttgatta tcgcgtcttt tgtgtcggtc attgtcgaac
    13801 tgttcaacac cgccgtcgaa gccgccgtcg atcatacttc gaccgaaaaa cacgagctgg
    13861 ccaaacgcgc caaagatgca ggttctgccg cacaactggt tgccatgctg atgttggcgg
    13921 cggtttggct gtccgccctg ttcgggtaaa acgcttgcag caggatttgt aatcctttag
    13981 gattagtatt tattattcac ttaatctaca tcaaatttcc gagcagtatt tgtatgtaag
    14041 attaagcaca ttccccgtct gatattaaag gagcaggaag atgaaaaaat tattggcagc
    14101 cgtgatgatg gcaggtttgg caggcgcggt ttccgccgcc ggagtccatg tcgaggacgg
    14161 ctgggcgcgc accactgtcg aaggtatgaa aatgggcggc gcgttcatga aaatccacaa
    14221 cgacgaagcc aaacaagact ttttgctcgg cggaagcagc cccgttgccg accgcgtcga
    14281 agtgcatacc cacatcaacg acaacggcgt gatgcgtatg cgcgaagtca aaggcggcgt
    14341 gcctttggag gcgaaatccg ttaccgaact caaacccggc agctatcacg tgatgtttat
    14401 gggtttgaaa aaacaactga aagagggcga caagattccc gttaccctga aatttaaaaa
    14461 cgccaaagcg caaaccgtcc aactggaagt caaaaccgcg ccgatgccgg caatgaacca
    14521 cggtcatcac cacggcgaag cgcatcagca ctaatctgct ggaaatattt gaaatgccgt
    14581 ctgaaaaagc ccgggctttc agacggcatt tttatgcccg cctttaaaat gtgttaaaat
    14641 ccgcctttaa aaaccgccgt ttccaagcca tcctgcgtat gaatacgaca tcaaacactt
    14701 ccaatatcat cgtcgggctt tccggcggtg tcgattcttc cgtaaccgcc gccctgctca
    14761 agcagcaggg ttatcaagtg cgcggtgtgt tcatgcagaa ctgggaaaac gacgacaacg
    14821 acgaatattg cagcatcaaa caggattcgt tcgatgccat cgccgttgcc gatattgtcg
    14881 gcatcgacat cgacatcgtt aatttcgccg cgcaatataa agacaaagtt tttgcttatt
    14941 ttcttcagga atacagtgcg gggcgcacgc cgaatccgga tgtgttgtgc aatgccgaaa
    15001 tcaaattcaa atgctttttg gactacgccg tagggcaggg cgcggatacc attgccaccg
    15061 gacactatgc gcgcaaagaa gtccgcaacg gcgtgcatta cctgctcaaa ggtttggatc
    15121 gaaacaaaga ccaaagctat tttctctacc gcctcaagcc tttccaactc gaacgcgcga
    15181 tttttccgtt gggcggtttg gaaaaacccg aagtgcgccg ccttgccgcc gaatttaatt
    15241 tgccgactgc cgctaaaaaa gacagtaccg gcatctgttt catcggcgag cgtccgttcc
    15301 gcgagtttct gcagaaatac ttgccgaccg acaacggcaa aatggtgacg cccgaaggga
    15361 aaaccatcgg cgaacacgtc gggctgatgt tttacacatt gggtcagcgc aaaggattgg
    15421 gcatcggcgg cgcgggcgaa ccgtggtttg ttgcggctaa agatttgacg aaaaacgaac
    15481 tcatcgtcgt acaaggacac gaccatccgc tgctctatac ccgcagcctt gtgatgaacg
    15541 atttgagttt cacgctgccc gaacgtccga aggcaggacg ctatacctgc aaaacgcgtt
    15601 accgtatggc ggacgcgcct tgcgaattgt gctatttgga tgatgaaacc gccgagctgg
    15661 tgtttgacga accgcaatgg gcggttacgc cgggtcagtc cgccgtgctg tacgacgtcg
    15721 acatctgttt gggcggcggc atcatccaaa cgaccgacaa acccgtcatc atcacgcgat
    15781 aaaggtaatg ccgtctgaaa cggttttcag acggcattgt tccgctcaat tccactttaa
    15841 agaccgatac ctatggaaaa aatctggtta gaaagctacg agaagggcgt cagtgccgaa
    15901 atcgacatca cgcaatacaa ttccgtcagc gacgtattcc gccaaagcgt ggaaaaattt
    15961 gcccgtctgc ccgcttttca aaatatgggc aaaacgctca cttatgccga aaccggcaaa
    16021 ctggcgaccg atttcgcctc ttatctgcaa aacgtcctca agctgccgcg cggcgaacgt
    16081 gttgccatta tgatgccgaa cgtattgcaa tatccgattg cccttttcgg tattttgcag
    16141 gcaggtttgg tggcggtgaa caccaatccg ctctatacgc cgcgcgagtt ggagcatcag
    16201 ctgaaagaca gcggtgcgac cgccatcatc gttttggaaa atttcgccaa cacgctggag
    16261 ctggtgctgc cgcgcacgca gatcaaacac gtcatcgtcg cctccgtcgg cgaaatgttc
    16321 gggctgctta aaggttcgct gatcaatttc atcatccgaa aaatcaagaa aatggttccc
    16381 gaataccgta ttcgggaaac cgtttccttt cagacggcat tgaaagaggg ggcgaagcac
    16441 gttttccaac ctgtcgcatt aaaccgcgaa gataccgcat tgttgcaata cacgggcggc
    16501 acgacaggcg ttgccagagg cgcggtgctg agccacggca acatctgcgc caatatgctt
    16561 caggcaaaag aatggattaa aaaccaattg cgcgagggaa aagaaaccgt tatcgccgcc
    16621 ctgccgctgt accacatctt cgccctgaca gtgaatctga tgatttttgc caatgccggc
    16681 tcgaaaattg tcctgattgc caacccgcgc gatatgaaag gctttatcgg cgaactgaaa
    16741 aagcagcggg ttaacgtatt tatcggcgtg aacacgctgt ttaacgcgat ggtcaaccgg
    16801 cccgatttcg ccgaagtcga tttttcagga ttgcggctga ctttgggcgg cggtatggcg
    16861 acccaaaaag ccgttgccga aaaatggaaa aaaatcaccg gcacgcccat cgtcgaagcc
    16921 tacggtttga ccgaagccag ccccggcgtg tgctgcaacc ccttaaacat cgaatcatac
    16981 agcggcagca tcggtttgcc cgtcccgtcc accgaagtcg aactgcgcga cgcaaacggc
    17041 aaagaagtcc ccgttgggca gccgggcgaa ttgtgggtaa aaggcccgca agtgatgcaa
    17101 ggctactgga accgccccga agaaaccgcc aaagccatag acgcgtgcgg ctttttggaa
    17161 accggcgata ttgccgtgat ggacgaaaaa ggccgtctga agctggtcga tcgcaaaaaa
    17221 gacctcgtcg tcgtttccgg attcaatgtt tatccgaacg aaatcgagga attcatcgcg
    17281 caccacgaaa aagttatgga agttgcgtgt atcggcgttc cgaacgaaaa aaccggcgag
    17341 gcactcaaag tgttcgtcgt caaaaaagac ccgtctttga ccaaagaaga actcaccgct
    17401 ttctgccgta ccggtctgac cgcatacaaa gtgccgaaag acatcgaatt ccgcgacgag
    17461 ttgcccaagt ccaatgtcgg caaaatcctg cgccgcgagt tgcgccaaag tgccgggaaa
    17521 taaagaaaag ataccgtctg aaaacagccg tccaccgttc agacggcatc cgtccgtttg
    17581 ccagaaccgc gcgcttcacg ttaaaatcac gcattccaac atgggtattc catcatgacc
    17641 aaattcattt tcgtaaccgg cggcgttgtc tcctcactgg gtaaaggtat cgccgccgct
    17701 tctattgccg ccatcctcga atcgcgcggc ttgaacgtta ccatgctcaa gcttgatcct
    17761 tatatcaacg tcgatcccgg cacgatgagc ccgttccaac acggcgaagt gttcgtaacc
    17821 gacgacggcg cggaaaccga cctcgacttg ggacactacg aacgtttcat cgattccacg
    17881 atgacccgcc gcaacagctt cagcacgggt caggtgtacg aaaacgttat cgccaaagaa
    17941 cgccgcggcg actatctcgg cggtacggtt caagtcatcc cgcacattac cgacgaaatc
    18001 aaacgacgca tccacgaagg cgcggcgggt tacgatgtgg cgattgtcga aatcggcggt
    18061 acggtcggcg acatcgaatc gctgccgttt ttggaagcca tccgccagat gcgaagccag
    18121 ttgggacgca acaacaccct gttcgcccac ttgagctacg ttccctacat cgccgccgca
    18181 ggcgaaatca aaaccaagcc gacccagcac accgtgaaag aaatgttgag catcggcttg
    18241 caacccgaca tcctgatttg ccgtatggac aggaaaatgc cggcagacga acgccgcaaa
    18301 atcgccttgt tctgcaacgt ggaagagcgc gcgattgtcg gcagctacga tgtggacagc
    18361 atctacgaat gccccgaaat gctgcacgac caaggcatcg acaacatcat taccgagcag
    18421 ttgcagctta acgtgcagca ggcggatttg accgcgtgga aaaaaatcgt ccacgccgtc
    18481 aaaaacccga aacacaccgt caaaatcgcg atggtcggca aatacgttga tttgaccgaa
    18541 tcctacaaat cattgattga agccttgaaa cacgcgggca tccataccga aaccgatgtg
    18601 cagattacct tcgttgacag cgaaagcatc gagaaaaaca agggcgacgt ttccgtactc
    18661 aaagatatgg atgccatcct cgttcccggc ggcttcggtt cgcgcggcgt ggaaggcaaa
    18721 atcgccgccg tgcgctacgc ccgtgaaaac aacgtgccat acttgggcat ctgcctcggt
    18781 atgcagattg cgctgattga atacgcccgc gacgtggcag gtttgaaagg tgcgaattcc
    18841 actgagtttg acttgaaatg cgctgccccc gtcgtcgccc tgattgacga atggcaaacc
    18901 gccgacggca gcgtcgaaac ccgtgacgaa tccgccgatt tgggcggcac gatgcgtttg
    18961 ggcgcgcaag aagtcgaatt gaaagcaggc agcctcgccg tcaaaatcta cggcagcgga
    19021 cacattcgcg aacgccaccg ccaccgctac gaagtcaaca acaactacgt ttccgcgctg
    19081 gaacaggcag gtttggtcat cggcggcgta tccgccggac gcgaacgctt ggtcgaaacc
    19141 atcgaactgc cgaaccatcc ttggttcttc gcctgccagt tccatcccga gttcacgtcc
    19201 aacccgcgca aagggcatcc tttgttcacc gcgtttgtca aagccgcgtt gaacaataaa
    19261 aaagcctgat aaagcgttac ttgatgatca aaatgccgtc tgaaagcctg acaaagactt
    19321 tcagacggca ttttcgcaaa tcggggatca taaaagccat caatttaatt tgggtctgta
    19381 ccagattagc agatatgtta ccctcgaaat atgaagataa cacactgcaa attaaagaaa
    19441 gaagtacaga aagaaccgct ccgttctttt gtaccggaag ttaccgcccg ttctgccgcc
    19501 gatattttgg gtatccatcc cgattcggcg gcactgtttt accgtaaaat ccgcacggtt
    19561 gccaaccatc g
//
LOCUS       DBJGGI010000034        18671 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.034, whole genome shotgun sequence.
ACCESSION   DBJGGI010000034 DBJGGI010000000
VERSION     DBJGGI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18671
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.034"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(240..1090)
                     /locus_tag="ACSIGJ_001238"
                     /pseudo
     CDS             complement(240..1090)
                     /locus_tag="ACSIGJ_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
     gene            1440..2954
                     /gene="katA"
                     /locus_tag="ACSIGJ_001239"
     CDS             1440..2954
                     /gene="katA"
                     /locus_tag="ACSIGJ_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HHQ1330102.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLTQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPASYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRSQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGIGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFDNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQAGLL"
     gene            3470..4036
                     /locus_tag="ACSIGJ_001240"
                     /pseudo
     CDS             3470..4036
                     /locus_tag="ACSIGJ_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
     gene            4258..5421
                     /locus_tag="ACSIGJ_001241"
     CDS             4258..5421
                     /locus_tag="ACSIGJ_001241"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HHQ1330103.1"
                     /translation="MSFKLRYLASVLALSSLLAACGGQEKSAAGDASPASETEAASQV
                     QASEAVPSASSASPEDQDLLKRAQGVFQPLPTVEEMQKIRPFTEEQVKLGHQLWYEPR
                     LSKGNTVSCNSCHNLASAGVDNMPTSQGHKGQFGGRNSPTALNAALLGSQFWDGRAAD
                     VEEQAGGPLVNPVEMANDSQEAAAAKIAKVPEYQEMFKKAFPEDGAVSFKNITTALGA
                     FERTLLTPTKWDEYLKGNVNALSEQERKGVRAFMDNGCIACHNGVNLGGTTFQKFGLV
                     QGPYWKFIEDPKRDKGRADVTKKTEDEFFFRVPGLRNVAKTYPYFHNGSVWELDKAVT
                     IMGKAQLGKDIPKEDVDNIVVFLNALSGNVSESARTMPELPLTAPMESKPDNK"
     gene            complement(6002..8038)
                     /locus_tag="ACSIGJ_001242"
     CDS             complement(6002..8038)
                     /locus_tag="ACSIGJ_001242"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HHQ1330104.1"
                     /translation="MIDNALLHLGEEPRFNQIKTEDIKPAVQTAIAEARGQIAAVKAQ
                     THTGWANTVERLTGITERVGRIWGVVSHLNSVVDTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFATLSPAQKTKLDHDLRDFVLSGAELPPERQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYAGNRELREQIYRAYVTRASELSNDGKFDNTANIDRTLENALKTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFAREHLGLADPQPWDLS
                     YAGEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFAEKTVPVWHKDVRYF
                     ELEQNGKTIGGVYMDLYAREGKRGGAWMNDYKGRRRFADGTLQLPTAYLVCNFAPPVG
                     GKEARLSHDEILTLFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGEPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMTIYSEDNEGR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFANSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
     gene            complement(8126..8998)
                     /locus_tag="ACSIGJ_001243"
     CDS             complement(8126..8998)
                     /locus_tag="ACSIGJ_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HHQ1330105.1"
                     /translation="MEIWNMLNTWPDAVPIRAEAAESVAAVAALLLARALLLNIHFRR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RYLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNHPAGSETL"
     gene            9264..11654
                     /gene="gyrB"
                     /locus_tag="ACSIGJ_001244"
     CDS             9264..11654
                     /gene="gyrB"
                     /locus_tag="ACSIGJ_001244"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HHQ1330106.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKI
                     VGDSDKKGTTVRFLAGTETFGNIEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVINQALTDFLEENPNEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVATLITALGAGIGKEEFNPEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEAALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGLVGEGAKLYKGENEYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            11753..13138
                     /locus_tag="ACSIGJ_001245"
     CDS             11753..13138
                     /locus_tag="ACSIGJ_001245"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HHQ1330107.1"
                     /translation="MISIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQTARIAIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLGRIRTQHILRLNGQEIRFIP
                     DRDLNIRGNQVLPKHPNGLRFTAYASDGTVLKEQVYYSVGGGFVVTEEDFEWQSETEK
                     AVPYPYNSCAGLLARCRLNRLDISEVVLANEAALAGCGEAEIRRRVAGVAEIMEECIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(13209..13307)
                     /locus_tag="ACSIGJ_001246"
     CDS             complement(13209..13307)
                     /locus_tag="ACSIGJ_001246"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HHQ1330108.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(13879..13955)
                     /locus_tag="ACSIGJ_001247"
     tRNA            complement(13879..13955)
                     /locus_tag="ACSIGJ_001247"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13919..13921),aa:Asp,seq:gtc)
     gene            complement(13988..14063)
                     /locus_tag="ACSIGJ_001248"
     tRNA            complement(13988..14063)
                     /locus_tag="ACSIGJ_001248"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14028..14030),aa:Val,seq:tac)
     gene            complement(14103..14179)
                     /locus_tag="ACSIGJ_001249"
     tRNA            complement(14103..14179)
                     /locus_tag="ACSIGJ_001249"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14143..14145),aa:Asp,seq:gtc)
     gene            complement(14212..14287)
                     /locus_tag="ACSIGJ_001250"
     tRNA            complement(14212..14287)
                     /locus_tag="ACSIGJ_001250"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14252..14254),aa:Val,seq:tac)
     gene            complement(14357..16333)
                     /locus_tag="ACSIGJ_001251"
     CDS             complement(14357..16333)
                     /locus_tag="ACSIGJ_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HHQ1330109.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPELEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVEAMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMHPNMPVYARATN
                     DDYVKTFTDMGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            16479..17318
                     /locus_tag="ACSIGJ_001252"
     CDS             16479..17318
                     /locus_tag="ACSIGJ_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HHQ1330110.1"
                     /translation="MTATAADLDRLLPQTQCRECGYEGCLPYARAMLRGEAHNLCAPG
                     GATVVRDLAALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVITDECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSDDSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPEKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRQGYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            17517..18548
                     /gene="gap"
                     /locus_tag="ACSIGJ_001253"
     CDS             17517..18548
                     /gene="gap"
                     /locus_tag="ACSIGJ_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HHQ1330111.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKIHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAVSEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMVKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
BASE COUNT         4475 a         5350 c         4911 g         3935 t
ORIGIN      
        1 ccaacctcac cggctcaaac cggcacagca acttatatcc cgtcattctc gcgcaggcgg
       61 gaatccagcc ccgccggtac agaaacttat cgaaaaaaag tttcttcaga ttttacgttc
      121 tagattccca ctttcgcggg aatgacggga tgcaggtttc cgcgcggacg gattcggcat
      181 tcccgctttc gcagaaatgt cggcttttgg tttgttgtct ttataagatt attcggtaat
      241 tattctgcgg ttttgcgttt gtgtgctgcc ggaatctcaa gggattctct gtatttggca
      301 acggtgcggc gggaaacctc tataccgcgg aaagccagca ggttggcgag tgcctcgtca
      361 gaataggact tgggcgtgga cgaggcaaaa ccggcggcgg gaaaggggtt gagcgaagca
      421 atgaggccga gtgcggcttc gagtgtgccg ctgtcggttt ggggcaggcg ttttttattc
      481 gcgccggggt ttggctgcgg ttgccgtcaa tgctgtcgag ggcgtttcgg acgatatgca
      541 gggcggcggg tttggcagca cattcgcccg atctttctat ctgcaatatc aacgattcgg
      601 tcacatcggc ggcggccatg cctgccggat cgaatttttt caatgcggtc agggcttgtt
      661 tcagcattgc ttcatccaac atccactcca agggcgtatg gtccaggatg tcttcgatgc
      721 tgtcggtcag gtagccctgc tcgtcaagga aatcgataag gatgtggacg caggcggctt
      781 cttggtcgga aagggggtgt tcgcacgctt gcgcgtgcag gtattgcttg aaatcttctt
      841 cgccggcgat gttggacagc atatcttcgc cttcgtctcc gccgatttga cgcgcgggcg
      901 cggtgtaatg gctgaattcg gcatcggaaa attcatccgt ttctttgcgt tcgagcaggg
      961 ggttgtccga cggccagttt tcgacctcgc gttcaagttc gatacccggc atctgcaata
     1021 cgcgcaaaga ttgttgcagc cgctggtcga gctgctgggt ctgtttgagc tttattccga
     1081 ttaaggtaat gataatgcgg taaaaattgt tattttcagc ctgtcggcgc aaaaaaatgc
     1141 cgcaaagcgt cattgcatta taaatggttt taatgagcgg gttcggattc cgtccgataa
     1201 caaaaaacaa acgaaaatgg aaacttgatt gcttataaca atattaaatc aatttcatag
     1261 ttttaataac gaaaatcttg gcgtataatc gcatccatag tttttgcaaa aggaaaataa
     1321 aatgtcgatt caagaaattt attacaatca agaaaccggt tacgaatacg ctttccgcca
     1381 aatcgtactg taagccgttt gccccccatt tgaccaacct gagaaaagga acaagagcga
     1441 tgactacctc caaatgcccc gtaacccatc tgaccatgaa caacggcgcg cccgttgccg
     1501 acaatcaaaa cagcctgacc gccggcccgc gcggccccct gctgacgcaa gatttgtggc
     1561 tgaatgaaaa actcgccgac ttcgtgcgcg aagtcatccc cgaacgccgt atgcacgcca
     1621 aaggttcggg cgcgttcggt acgtttaccg taacacacga catcaccaaa tacacccgcg
     1681 ccaaaatttt cagcgaagtc ggcaaaaaaa ccgagatgtt cgcccgtttc accaccgtgg
     1741 caggcgagcg cggcgcagcc gatgcggaac gcgacatccg cggttttgcg ttgaaattct
     1801 ataccgaaga aggcaactgg gatgtggtcg gcaacaacac gcccgtgttc ttcctgcgcg
     1861 acccgcgcaa attccccgac ctgaacaaag ccgtcaaacg cgatccgcgc accaatatgc
     1921 gttccgccac aaacaactgg gacttctgga cgctgctgcc cgaagccctg caccaagtta
     1981 ccatcgtgat gagcgaccgc ggtattcccg ccagctaccg ccatatgcac ggcttcggtt
     2041 cgcataccta cagcttctgg aacgaagcgg gcgaacgttt ttgggtgaaa ttccatttcc
     2101 gcagccagca agggattaaa aacctgacca acgaagaagc tgcaaaaatc atcgccgacg
     2161 accgcgaaag ccatcagcgc gacctgtacg aagccatcga gcgcggcgag ttcccgaaat
     2221 ggacgatgta catccaagtc atgcctgaag ccgatgccga aaaagtgcct tatcatccgt
     2281 ttgacttgac caaagtttgg ccgaaaaaag actatccgct gattgaagtg ggcgaattcg
     2341 agttgaaccg caatcccgaa aacttcttcg ccgatgtgga acaatccgcc ttcgcaccga
     2401 gcaacctcgt tcccggtatc ggcgcgagcc cggataaaat gttgcaagcc cgtttgttca
     2461 attacgccga cgcgcaacgt taccgcttgg gtgtgaactt ccgccaaatc cccgtcaatc
     2521 gtccgcgttg ccctgttcac agcaaccagc gcgacggaca aggccgcgcc gacggcaact
     2581 acggcagcct gccgcactac gaacccaaca gcttcggcca atggcagcaa caacccgact
     2641 tcgccgaacc gcctttgaaa atcaacggcg acgcggcaca ctgggactac cgccaagacg
     2701 atgacgacta cttcagccaa ccgcgcgcct tgttcaacct gatgaacgac gcgcagaaac
     2761 aggctttgtt tgacaacacc gccgcagcca tgggcgatgc gcccgacttc atcaaatacc
     2821 gccatatccg caactgctac cgttgcgacc cggcatacgg tgaaggcgta gccaaagccc
     2881 tcggactgac cgtcgaagac gcacaagccg cccgcgcgac cgatcccgca ctgggtcagg
     2941 ccggtttgct gtaagggggc attatgtgga tggaaattga agaaatcctg tcccgcgccg
     3001 tccgataccg gaaataaccg ggcataaaaa tgccgtctga aacgtcgttt gaccgtttca
     3061 gacggcattt ccacacctcg ccccgccgtt tcagtgggcg ttttttatta tagtggatta
     3121 aatttaaacc ggtacggcgt tgcctcgcct tgtcctgatt tttgttaatc cactataaac
     3181 gcaaaatatc ccgtcattcc cgcaccatcc ccaccggttc aaaccggcac gaaaactttt
     3241 ccgcgtcatt cccgcgaaag tgggaatccg gaacgtaaaa tctaaagaaa ccgttttatc
     3301 cgataagttt ccgtgccgac ggggctggat tcccactttc gtgggaatga cgggatttct
     3361 gtttttgatt ttttgttttt gcgggaataa cgggatgcgg gttttcgtgc ggcgtttttc
     3421 gcgctttgct gttttgttat aatccgccct ttttaaggat gggtgcggta tgagtcttta
     3481 cgctttgctc ttggttgcgc tgggaatgtc gatggatgcg tttgccgtcg cgctggcaaa
     3541 gggtgcggcg gtcaggatgc ccccgcgcaa aattgcagca acggctttgg tgttcggtac
     3601 ggttgaagcg ttcatgccgc tggcgggctg ggtaggcggt ttttatgcca agccgtttat
     3661 cagcgaatga gaccattggg tggcttttgt cctgttgggc ggactgggtc tgaaaatgat
     3721 gcgcgaaggg ttgtccggcg aggcggaaga tgtgcgcgaa agcaaacagg aaagcctatg
     3781 gatgacggtt ttgactgctt ttggaaccag tattgattcc atgatcgtcg gggtgggctt
     3841 ggcgtttatg gaggtaaaca tcgcctttgc cgccgccgta atcggtatgg cggctacggt
     3901 aatggttacc atcgggctga cggcgggaaa ggcttttggc gtattgttcg gcaggcgtgc
     3961 ggaatttgcc ggaggtttgg tgttgattgc catcggtaca tggacgctct tatcgcattt
     4021 gggtttgatt caatgatgtc ggaaaataaa atgccgtctg aagctttacc ggtttcagac
     4081 ggcattttgt taaacttgat tctctatcgg tattattgtg taaagctatt gaaaataaaa
     4141 tattgatttt ccgcaaagat ggctaaaggt gcgataggta cactgaagaa ataatcggca
     4201 gaacaggttt gcctgccggt ttgaacaata attttaacct cccataagga atacacgatg
     4261 tctttcaaac tccgttacct cgcatcagta ttggcattgt cttcactgtt ggcggcatgc
     4321 ggcggtcagg aaaagtctgc ggcaggcgat gcttctcctg cttccgagac cgaagcggct
     4381 tcccaagtac aggcctcgga agccgttcct tccgcttcat cggcctcgcc cgaagaccaa
     4441 gaccttttga aacgcgcgca aggtgtattc caacctttgc cgactgtcga agaaatgcag
     4501 aaaatccgtc cgtttaccga agaacaggtc aaactcggac accaactctg gtatgaacca
     4561 cgcctttcca aaggcaatac cgtaagctgc aactcttgcc acaaccttgc ttccgccggt
     4621 gtggacaata tgccgaccag tcaggggcac aaagggcagt tcggcggacg caactcgcct
     4681 accgcattga atgctgcgct gctgggcagc cagttttggg acggacgtgc cgccgatgtt
     4741 gaagaacagg ctggcgggcc tttggtgaat ccggtggaaa tggcgaatga ttcgcaagag
     4801 gcggctgcag ccaaaatcgc caaagttccc gaatatcaag aaatgtttaa aaaagctttt
     4861 cctgaagacg gcgcggtttc gtttaaaaac atcactaccg cattgggtgc gtttgagcgt
     4921 accctgctga cgccgaccaa atgggacgaa tacctcaaag gcaacgtcaa cgccctgagc
     4981 gaacaggaac gaaaaggcgt gcgcgcgttc atggacaacg gctgtattgc ctgccacaac
     5041 ggtgtcaacc ttggaggcac gaccttccag aaattcggtc tggtccaagg gccgtattgg
     5101 aaattcattg aagatccgaa acgcgacaaa ggccgtgctg acgtaaccaa aaaaaccgaa
     5161 gacgaatttt tcttccgtgt tccggggttg cgtaacgtgg ctaaaactta tccgtatttc
     5221 cacaacggca gcgtgtggga gctggataag gcagttacca tcatgggtaa ggcgcaattg
     5281 ggtaaagaca ttccgaaaga agatgtggat aacatcgtcg tattcctgaa tgcactttcc
     5341 ggcaatgttt ccgaatcagc gcgcacgatg ccggaactgc ccctgaccgc accgatggaa
     5401 tctaagccgg acaacaaata atccggccgg tacgcaaaaa tgccgtctga aacgggaatc
     5461 tgtttcagac ggcattttat atttcgtcgc gtcagatacg cagggtatgc cgtagggtgt
     5521 gcttcagcca gccgtttcca ccaattcaac caaccatgtc cgggtcttat gcgtttttca
     5581 cgaatgcgcg gcgtaggcgg agggattggt gggctgaagc ctaattcaat ggctgcgttg
     5641 tgtaagccat gatgcgtttg gataattaat caatattctt gcgctaccat taccatcggc
     5701 tttgggggtt tgtacttcgg cattatgata atcaatagcc tgtttggatt gtattcccga
     5761 atccgacagt tttagattat aaaaaggccg tctgaaaaat tttcagacgg ccttttatag
     5821 taaatttcaa ataaaaatat ctttgtcgtc attcccacgc aggcgggaat ccagacttgt
     5881 cggcacagaa acttatcggg taaaacggtt tcttcaattt tatgttccgg attcccgcct
     5941 gcgcggggat aacgattcag gtatttctac atcgaatccg ctttttatat caacctgact
     6001 atcaagccgc gttgtcgaag ccgctgtggc gcagcagtgc gtctatgctc ggttcgcgtc
     6061 cgcggaaggc cttgaaggat tccgccgcgc tgcgggagcc gccgacggcg aggatttcct
     6121 gccagaagcg tttgcctgtg gcggcaacgt cgtcgctttc ttcaaaggcg gcgtaggcgt
     6181 cggcgctgag gacttccgcc catgcgtagc tgtaatagcc tgcggagtag ccgccggcga
     6241 agatgtggcc gaagctgttg gcgaagcggt tgtattcggg cggttggatg acggcgactt
     6301 ctttgcgcac gctgtctaaa acctgctgcc agtttttcag acggccttcg ttgtcttcgc
     6361 tgtaaatggt catgtcaaag agggcgaatt ccatttggcg gacgaggaac atgccgcgtt
     6421 ggaagttttt ggcggcgagc attttgtcga agagttcttt cggcaggggc tcgccggttt
     6481 cttcgtgggc ggacatttgt gccaatacat tgtattccca aacgaagttt tccataaact
     6541 ggctgggcag ttcgaccgcg tcccattcta cgccgttgat gccggacacg cccagttcgt
     6601 ccacttgggt aagcaggtgg tgcagtccgt ggccggtttc gtggaagagg gtgaggattt
     6661 cgtcgtggct taaacgcgct tctttgccgc cgacgggcgg ggcgaagttg cagacgaggt
     6721 aggcggtggg cagttgcagc gtgccgtcgg caaagcggcg gcggcctttg tagtcgttca
     6781 tccacgcgcc gccgcgtttg ccttcgcgtg cgtacaaatc catataaacg ccgccgatgg
     6841 ttttgccgtt ttgctccagc tcgaagtagc gcacgtcttt gtgccagacg gggacggttt
     6901 tttcggcaaa tccgatgccg tagagttttt tgatttgggc gaacagtccg tttaatactt
     6961 tgccgacggg gaagtatttt ttgacttcgg tttcgctgaa tgcgtatttg gcttcgcgca
     7021 gtttttcgcc ggcgtagctc aagtcccacg gctgcgggtc ggcgagaccg aggtgttcgc
     7081 gggcgaaggc tttgacttcg gcgaggtctt tttcggcgta gggtttggcg cggcgggcga
     7141 ggtcgtgcag gaagtttaaa acctgttcgg gcgtgtccgc cattttggtt gccagcgaca
     7201 attcggcgta atttttaaag ccgagcagtt tggcggtttt caatgcgttt tcgagcgtgc
     7261 ggtcgatgtt ggcggtgttg tcgaatttgc cgtcgtttga aagttcgctg gcacgggtaa
     7321 cgtaggcgcg gtagatttgt tcgcgcagtt cgcggttgcc ggcgtattgg atgacggcaa
     7381 ggtagtgcgg aatctgcaag ccgattttgt aacctgtttt gccttcgctt tgcgcggcgg
     7441 cggcaaacat cgcgagcgcg tcttcgggaa tgccggcaag cggtgcggca tcgtcaaagt
     7501 aaatgccgaa cgcgtcggtc gcgtctagga cgttttggga gaatttggcg gaaagttgcg
     7561 cgccttcggt ttgcagtttt gccagttctg cctgccgttc gggcggcagt tccgcgccgc
     7621 tcaatacgaa atcgcgcagg tcgtgatcga gcttggtttt ttgtgcgggg gaaagcgttg
     7681 caaattcggg ggaatttttg atggttttga agcggttgta cagttcgatg tcttgtccga
     7741 tttcggtgaa gaagacggtg atttcaggca tcagttcgtt atagacggcg cgcagttcgg
     7801 gcgtgtcgac gacggagttg agatgggaca cgacgcccca aatcctgccg acgcgttcgg
     7861 tgatgccggt cagacgctcg acggtgttcg cccagccggt gtgcgtttgc gctttgacgg
     7921 cggcgatttg tccgcgcgct tcggcgatgg cggtttggac ggcgggtttg atgtcttcgg
     7981 ttttgatttg attaaaacgg ggttcttcgc ccaagtggag cagtgcgttg tcaatcataa
     8041 gatgggtttc ctttgcgtgt tgtgcttcag acggcatatc ggcaatatgc cgtctgaagc
     8101 cagaaatggg gccggccggg gaaagttaaa gtgtttcgga gccggcggga tgatttaaca
     8161 ggcggtattg tacgcgcaaa aattcgtcca taaccgcctg ttggatttcc agccgctttg
     8221 aaacggggga ggcgaagcgg acgatgatgc ggtatgcctt gtcgtcgtac ggtacgcggg
     8281 taacgcgcgg cctggcggcg ggcgtgataa acagtttttc cgcctgcacg ttttccaaat
     8341 accgctgaat ggcggggatg tagggcgcgc acaagggctc gagtacggct ttcagacggc
     8401 atacggcttc atccgaatcc aaatggatgg gaacggggat ttcgaccgta tggatgacat
     8461 agtcgcccaa aatattgtcg cggcgcacgg ggtggctcaa caacaggctg ttggggaaag
     8521 aaacggtggt tcccgcaagc tgtccgacca aggggttcgg accgacctgc atcatcagcg
     8581 tgttcaacag attgatgtcg accacgcgcc cgcgcaggcc gttgatttcg atatagtcgc
     8641 cgaccgagta ttgctgggtg gcagacctta aaatactgcc cgacagacac ataatcagtt
     8701 cttttgtcgc cacgacgacc gccgccgcca ccgcaaacat cgacaaagcc agcgtttgaa
     8761 tttgcgccga ccagataaat gccagcgaaa acagcaccaa aagcagcgtt atattgcggc
     8821 tggcaaccaa aaaccgccgc ttgctttcga tgccgaaatc cggatgccgt ctgaagtgga
     8881 tattcaacag aagggcgcgc gccagcagca aagccgcgac cgccgccacg gattcggccg
     8941 cctccgcgcg tatcgggacg gcatcgggcc aagtgttcaa catattccat atttccattt
     9001 ccgcgcccct gtccgaaaag tatagggtgt agattttagt ggcaaaaaaa cgcttttgcc
     9061 actttgccgg caaattccgc gcccgtaccg gccgcaaatg cgccgcacgc cctttcagac
     9121 ggcatccgga tgcggataac gctgaatcag gcggcgaaat cggctataat acacaattat
     9181 atgcgccgtc ccgcccgctt tttccacaag gtgcggactg ttttggcggc atcgcaaaat
     9241 cttttcaaaa tccggcaaaa aatatgactg aacaaaaaca cgaagaatac ggcgccgaca
     9301 gcatccaggt gctcgaaggc ttggaagcgg tacgcaaacg ccccggcatg tacatcggcg
     9361 acacgcagga cggcagcggg ctgcaccata tggtgtttga agtattggac aacgccatcg
     9421 acgaagcact cgccggacat tgcgacaaaa tcacggtaac gatacacgcc gaccattccg
     9481 tcagcgtcgc cgacaacggg cgcggtatgc ccaccggcat ccacccgaaa gaagggcgtt
     9541 ccgccgccga agtcatcatg accgtcttgc acgcgggcgg caaattcgac aacaacagct
     9601 acaaaatctc cggcggcctg cacggcgtgg gcgtatccgt cgtcaacgcg ctgtccgact
     9661 gggtaacgct gaccatctac cgcgacggca aagaacactt cgtccgcttc gtacgcggcg
     9721 aaaccgaaga gccgctgaaa attgtcggcg attccgacaa aaaaggcacg accgtgcgct
     9781 tcctcgccgg cacggaaacc ttcggcaata tcgaatacag cttcgacatc ctcgccaaac
     9841 gtattcgcga actttcgttc ctaaacaacg gcgtggacat cgaattgacc gacgagcgcg
     9901 acggcaagca cgaaagcttc gccctttccg gcggcgtggc gggcttcgtg caatacatga
     9961 accgcaaaaa aacgcccttg cacgaaaaaa tcttctatgc gttcggcgag aaagacggca
    10021 tgagcgtcga atgcgcaatg caatggaacg acagctatca ggaaagcgtg cagtgcttca
    10081 ccaacaacat ccctcagcgc gacggcggta cgcacctgac cgcgctgcgc caagtgatga
    10141 cgcgcaccat caacagctac atcgaagcta acgaagtcgc caaaaaagcc aaagtggaaa
    10201 ccgccggcga cgatatgcgc gaaggtttga cctgcgtgtt gtccgtcaaa ctgcccgacc
    10261 ccaaattctc atcccaaacc aaagacaaac tggtttccgg cgaaatcggc cccgttgtca
    10321 acgaagtcat caaccaagca ctaaccgact tcctcgaaga aaatccgaac gaagccaaaa
    10381 tcatcaccgg caaaatcgtc gatgccgccc gcgcacgcga agccgcccgc aaagcccgcg
    10441 aaatcacccg ccgcaaaggc gtgatggacg gcttgggact gcccggcaaa ctcgccgact
    10501 gccaagaaaa agaccctgcc ctgtctgaac tctacctcgt cgagggcgac tccgcaggcg
    10561 gttccgccat gcagggccgc gaccgcaaat tccaagcgat tttgccgctc aaaggtaaaa
    10621 ttttgaacgt cgaaaaagca cgttttgaaa aaatgctcgc cagccaagag gtcgccaccc
    10681 tgattaccgc gctgggtgca ggcatcggca aagaagagtt caaccctgaa aaactacgct
    10741 accaccgcat catcatcatg accgatgccg acgtggacgg tgcgcacatc cgcaccctgc
    10801 tcctgacctt cttctaccgc caaatgcccg aactggtcga gcgcggctac atttacatcg
    10861 cccagccgcc gctctacaaa gccaaatacg gcaagcagga gcgttacctc aaagacgaac
    10921 tggaaaaaga ccaatggctg ctcggccttg ccttggaaaa agccaaaatc gtttcagacg
    10981 gccgcaccat cgaaggcgca gaacttgccg acaccgccaa acaattcttg ttggcgaaaa
    11041 ccgtcatcga acaggaaagc cgcttcgtgg acgaactcgt cctgcgtgcc atgctgcacg
    11101 cgtcgcccat tgatttgacg tcgtctgaaa acgccgataa agccgttgcc gaactttccg
    11161 gtttgcttga cgaaaaagaa gccgccctcg aacgcatcga aggtcatgaa ggacaccggt
    11221 tcatcaaaat cacgcgcaag ctgcacggca acgtcatggt cagctacatc gaacccaagt
    11281 tcctcaacag caaagcctac caaaccctca cccaaaccgc cgccgcgctc aaaggcttgg
    11341 tcggcgaggg cgccaagctc tacaaaggcg agaacgagta cgacgcggac agctttgaaa
    11401 ccgctttgga catcttgatg agcgttgccc aaaaaggtat gtccatccaa cgatacaaag
    11461 gtttgggcga gatgaacccc gagcagcttt gggaaaccac gatggatccc gccgtgcgcc
    11521 gcctgttgaa agtgcgcatc gaagatgcca ttgccgccga cgaagtgttc gttaccctga
    11581 tgggcgacga ggtcgaaccg cgccgcgcct ttatcgaaaa caatgcgctg attgcgcaaa
    11641 atatcgacgc ataagtgccg ttttaaaaaa ggagacgggc agcttgccgc gtctcctttt
    11701 ggtttgtcaa acggaagcca tgccgtctga aaccgccttt cggagcaaaa ccatgatcag
    11761 catttttgat attttcaaaa tcggtatcgg cccttccagt tcgcatacgg tcggcccgat
    11821 gaaggccgcc gccgcctttg ccgcaggctt ggacgcgcaa accgcccgca tcgccatcga
    11881 catttacggc tcgcttgccc tgaccggata cggacacggc acatttgacg cgctgatgct
    11941 cggtttggaa ggcagcctgc cgcacgacat cccgcttgcc ggcattcccg aacgcctcgg
    12001 acgcatccgc acgcagcaca tcctccggct caacgggcaa gaaatccgct tcatccccga
    12061 ccgcgacctg aacatacgcg gcaatcaagt gctgcccaaa caccccaacg gcctgcgatt
    12121 taccgcctat gcttcagacg gcacggtatt gaaggaacag gtttattatt cggtcggcgg
    12181 cggctttgtc gttaccgaag aagacttcga gtggcagtcg gaaacggaaa aagccgttcc
    12241 ttatccttac aacagttgtg ccgggctgct tgcccgatgc cgtctgaacc ggctcgacat
    12301 ctccgaagtc gtgttggcaa acgaagccgc gcttgccgga tgcggcgaag ccgaaatccg
    12361 ccgccgggtt gccggtgttg ccgagattat ggaagagtgc atcaaacgcg gcttgggtgc
    12421 ggacggcgaa ctgcccggcg gattgaacgt ccgccgccgc gccccgcagc ttgccgccaa
    12481 actcaaagtc ctgcgcgaaa ccgaaatcgt caacacccag ctctggccga tggtgtacgc
    12541 catggcggtc aacgaagaaa acgccgccgg cggacgcgtc gttaccgccc cgaccaacgg
    12601 cgcggcaggc atcattcccg ccgtattgca ttatttccgc aagttcaacc cccacgccac
    12661 acaggaacgt gtcgaaaact tcctgctcac cgcaggcgca atcggcatcc tctacaaaac
    12721 caacgcctcc atttccggcg cggatgtcgg ttgtcagggc gaagtcggcg tagcgtgttc
    12781 aatggcggcg ggtgcatacg ccgaagtcat cggcggcacg cccaaacaag tggaaaacgc
    12841 cgccgaaatg gcaatggaac accatttggg cttaacttgc gaccccgtcg gcgggttggt
    12901 gcaaatcccc tgcatcgaac gcaacggcat cgccgccgaa aaagccctca aactcggcac
    12961 gctcgcgctc ttggaagacg gcacggacaa aaaagtctcg ctcgacgaag tcatccgaac
    13021 catgctgcaa accggccggg acatgaaggc gacctacaaa gaaacctcgc ttgccggact
    13081 cgccgccacg ctccggaaaa aagccgtccc cgtatccgtg cgcgtggtcg agtgctgagg
    13141 atggacagaa acgaaaatgc cgtctgaaaa cagtttttca gacggcattt catttatttc
    13201 ttgcgggttt accgcttcaa cgccaacgtc aacacgcccg ccgtaaccag ccccaagcct
    13261 atccattcct gcgtgttcgg gcgttcgtcc aagaaaacca ccgccatcag gaatgcaaac
    13321 cggcgttgtt tgcagcatcc ggcaagaatt tttatagtgg attaacaaaa atcaggacaa
    13381 ggcgacgaag ccgcaggcag tacaaatagt acggaaccga ttcacttggt gcttcagcac
    13441 cttagagaat cgttctcttt gagctaaggc gaggcaacgc cgtaccggtt tttgttaatc
    13501 cactataaaa ggtcttttct aaaatatgca gatattcgaa tgtcgaattt gccttatgat
    13561 tacggttttc tgtcttatcc gccttaaaac gccggtattc cgtttgaacc aaattatatt
    13621 tgccgaaaca ctcaaaaatc tcccttacct gcccgacaga catcaagact tcgttgttgt
    13681 aactgagaaa aatatattga aagtccgcat ccctaatcaa agcctcaaat accggaacca
    13741 catccgattt tgatcaagtc cgcgctaaaa cacatcgtac agcgcaaaac cggatgggaa
    13801 tggcaaaatc cggaaacaaa catttttcaa agcgtacaaa caaaaaatac caaccggagg
    13861 ttggtatttc ttaacttttg gcgcggcgga cggggctcga acccgcgacc accggcgtga
    13921 caggccggta ctctaaccaa ctgagctacc accgcgcatc cattgtttgc accaatgaaa
    13981 ggaaacttgg tgggtgatga cggagtcgaa ccgccgacat tctgcttgta aggcagacgc
    14041 tctaccaact gagctaatca cccgtgtata aaaaataccg acagagtcag tattttgaaa
    14101 cttggcgcgg cggacggggc tcgaacccgc gaccaccggc gtgacaggcc ggtactctaa
    14161 ccaactgagc taccaccgcg catccattgt ttgcaacaat gaaaggaaac ttggtgggtg
    14221 atgacggagt cgaaccgccg acattctgct tgtaaggcag acgctctacc aactgagcta
    14281 atcacccttg cgatttgcga aaacgctatt agaccaaata accgggaatg gcgcaagcct
    14341 tatttcggca tttcatttat attccctgca agctccagtt ttcaaaagag ataatttctt
    14401 cttttttgcc tgcgaccacc aacacgtcac cgccttccaa tgtaaacgag gcatccgggt
    14461 tttcaatccg gccggtgcgg cggcggacga acaagagttt gatgccgtaa gccgccatcg
    14521 gaagcgtgcc gactgttttg ccgacggcgt atgcttctgc agccaaagga aaggcgtggc
    14581 ggacggtttc gccattttcg ccgaatcctg cctcatcatc actaccgaca aacagtccct
    14641 ccaacgcggc ataacggctg tggcggatat ttgccatcgt ctgatagacg tgccggtacg
    14701 acgcgccgtt acccaacatc gcatagcctg ccagcacgag tccggtttct ttggtgtccg
    14761 acacggcttc ttccgcaccc atatcggtaa acgttttcac ataatcgtca ttggtggcgc
    14821 gcgcatatac gggcatattg ggatgcatgg acagcacatt gtctaaaacg tgttgcgttt
    14881 cgtgcatatt gttgagcgta accaccacca ttttcgcccg tcccagaccg gcggcttcca
    14941 atacttccct gcgtttcgca tcgccgaacg acaccggctc gcctgcactt ctggctacct
    15001 gcacgcgtgc gatgtccaag tcgagcgcga aatacggaat atcctcttgg gcgaggacgc
    15061 gtccgaccgt ctgcccgccc ctgccgaagc cgacaatcag cacatgatca gacttgctca
    15121 tcgcttccac caacatgctg tgcagatcga gcgacttcat gtcccagctt gacttgacca
    15181 agcgcccgac cagcgcatcg ctgccgccca agaggaaggg cgcgataatc atcgacagca
    15241 gaaccgccgc cgtcgccgcc tgttccaatt ctggcgaaac catatcaagc tgcccggcaa
    15301 tggccagcat cacgaagccg aactcgccgc cttgcgcgag atacaaagcc gttttgaggc
    15361 tgtcgccgac cgaatgcttc attttgaatg caatggcaaa cacaaccaat gccttcaaca
    15421 ccagcagcat tgccaacagc atcaatacct gccgccagcc gccgatcaat gcctgaatgt
    15481 ccagcttcat gccgaccgtg ataaagaaga agccgagcaa aatatcgcgg aacgggcgaa
    15541 tgtcgtcttc gacttggaaa cggtattccg tttccgaaag caacatgccg gcaacgaatg
    15601 cgcccaacgc catagacaaa ccttccagct cagtcagata agccacaccc aaggttacca
    15661 gcagcacatt gatcataaag agttcggacg atttgcgttt tgccaccatc ctgaaccatc
    15721 gcgacataat tttgctgccg acgaaaaaca gcagccccag cgtcagcagc atttttgcaa
    15781 acgccaaacc caaggccgcc caaatatttc cgtcccctcc gccagccagc gcgggaatca
    15841 gaatcatcag cggcacgacg gcgatgtcct gcatcagcag cacgcccatc gccatctgac
    15901 cgtgcggctg ccccaactcc gtcttttccg acaaaatccg gctcacaatc gccgtggacg
    15961 acatcgccaa cgcgcccgat acggcaaacg cccaattgaa cggcacgccc gtcagcatca
    16021 gtatgcccat taccgacagc atcgtaacgc cgacctgcaa accgcccaga ccgaacacca
    16081 gccgcctcat cgccctcagc ttgggcaatg aaaactccaa accgatgctg aacatcagga
    16141 acacaatccc gatttcgccc aaataatccg tcgcatggct tttcggaatc aggctgagca
    16201 taccgggccc cgccaaaaag cccaccagca ggtagcccag catggaggga atgttgaact
    16261 tgcggcacag gatcaccgta atgaccgaca ccagcagaac aaccacaata ggggcaagcg
    16321 aaaattcgtt catggaccgt ccgaacagga aaatacagaa aaatgccgtc tgaaacggca
    16381 tacgccgccg tattataaca aaacaccgcg caccatccga aacgggcgcg gcatacaatt
    16441 ctgataaaat acgccctttt gacccgcacc accgcacaat gaccgccacc gccgccgatt
    16501 tagaccgact cctcccccaa acccaatgcc gcgaatgcgg ctatgaaggc tgcctgccct
    16561 acgcccgcgc gatgctgcgg ggcgaggcgc acaacctctg cgccccgggc ggcgcaaccg
    16621 tcgtccgcga ccttgccgcc ctgctcggca aaccccttgt cgcacctgcc aaaacccaag
    16681 ccaaagcact cgcccggata gacgaaaccg cctgtatcgg ctgcaccgcc tgcatccgcg
    16741 cctgccctgc cgatgccatt atgggcgcgg gcaaacttat gcacaccgtc atcaccgacg
    16801 aatgcaccgg ctgcggactc tgcgtcgccc cctgccccgt ggactgcatc catatgcagc
    16861 ccgttgccga caccgtcctg ccgcgcgcgc gccgtttcag cctgtccgac gacagccgtt
    16921 ttgccgccgc cgaacacgcg cgtgcgcgct acctcaaacg caacgaacgc aaacagcgcg
    16981 aagccgacga acgcaaggcc atgcttgccg aacgcgaagc cgccgtccgc aacgcgcgtc
    17041 cgcaaacgcc cgacacaccg gaaaaaccgg cgttcaaccc cgccgacctc atcgccaaag
    17101 ccatggcaaa agcgcaaacc caacaggatc gcctcgccgc cgccgacaac cgccaaggct
    17161 atcaggcaaa acagatagcc gaagcccgcg aacgcgccga attgcgccgc gcccaacgcg
    17221 atatgaaata cggcagcgac agcgaaaaag ccgccgccct cgaatacctc aaacaataca
    17281 aagccaaaca ggaagccgca cagaataccg cctcctgacc cttacccgac atgccgtctg
    17341 aagccgcttc agacggcatt ttatcaagct ctccgtccgc cgtacccgtg ccgtccgtat
    17401 cttaccgccc acccttccgg ccgtgccgtt ttcaataaaa tattaattac acgccactac
    17461 aaatttgcta taatccgccc cgaaaatcta cccaaccctc aacaaaggaa caaaccatgg
    17521 gcatcaaagt cgccatcaac ggctacggac gcatcggccg ccaggttttg cgcgccatct
    17581 acgactatca gattcaagac caactccaaa tcgtcgccgt caacgccagc ggcagccttg
    17641 aaaccaacgc ccatctgacc aaattcgaca ccgtacacgg acgctttgaa gccgacgtat
    17701 cccacgacgg cggcaacctc atcgtcaacg gcgacaaaat ccccttcttc tcgacccgca
    17761 accctgccga actgccgtgg aaagaactcg gtgtcgattt ggtcatggaa tgcaccggcg
    17821 cgttcaccag caaagaaaaa gccaaaatcc acctcgaaag cggcgcgaaa aaagtcctca
    17881 tttccgcacc gggcggcgac gatgtcgatg caaccgtcgt gtacggcgtg aacgacagcg
    17941 tcctgaccgc cgacatgacc gtcgtttcca acgcttcctg caccaccaac tgcctctcgc
    18001 cggttgccaa agtgttgagc gaaagcgtcg gcatcgtcaa aggcgcaatg accaccatcc
    18061 acgcactgac caacgaccaa accgttaccg acgtgcgcca caaagacctg cgccgcgccc
    18121 gcagcggcgt ggaaaacatg attccgacca aaaccggcgc ggcaaaagcc gtcggtttgg
    18181 tcttgcccga attgaaaggc agactcgacg ggcttgccat ccgcgtgccg accgtcaacg
    18241 tatccttggt agatttgagc ttccaagccg cgcgcgacac cacagtcgaa gaaatcaacg
    18301 cactgatgaa agccgtctcg gaagcaggcg cgctcaaagg cgttttgggc tacaacaccc
    18361 tgccattggt ttccatggac ttcaaccaca ccaccgaagc cagccacttc gacgcaacgc
    18421 tgaccaaagt cgtcgacggc aacatggtca aagtgttcgc gtggtatgac aacgaatggg
    18481 gcttcagctg ccaaatgctg aacaccgcac gccgtatgtt cggacttgaa gtgcgcccgc
    18541 tcaaataagc aacaaaccgt caaacaaaat gccgtctgaa acccgatgtt ttcaagtttc
    18601 agacggcact tttcattttt gaccgcgatt ttacccggct gtcgtcattt ccaattttat
    18661 agtggattaa a
//
LOCUS       DBJGGI010000035        18593 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.035, whole genome shotgun sequence.
ACCESSION   DBJGGI010000035 DBJGGI010000000
VERSION     DBJGGI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18593
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.035"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..81
                     /locus_tag="ACSIGJ_001254"
     CDS             <1..81
                     /locus_tag="ACSIGJ_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HHQ1330112.1"
                     /translation="LRECEFRLNFGTPSRQLKILRDRCGI"
     gene            complement(155..1576)
                     /locus_tag="ACSIGJ_001255"
     CDS             complement(155..1576)
                     /locus_tag="ACSIGJ_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HHQ1330113.1"
                     /translation="MRHLPLFSLMLFPASVYAADSDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQDRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILITLTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINTMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGPLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLVFFVFKLL"
     gene            complement(1591..1971)
                     /locus_tag="ACSIGJ_001256"
     CDS             complement(1591..1971)
                     /locus_tag="ACSIGJ_001256"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HHQ1330114.1"
                     /translation="MSPYGCGAGPNLECRTERDKGKQTGGKPVSLQIYVKINSFFVFD
                     RLFIKDIYLFVLNKIIYQKEHIFPSTAIRLPAAFRFGKLVYPDYLLYNQSENFFQKQT
                     GSTVRLCPGGTVPVSLPRAAQGSA"
     gene            complement(1934..2293)
                     /locus_tag="ACSIGJ_001257"
     CDS             complement(1934..2293)
                     /locus_tag="ACSIGJ_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HHQ1330115.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2290..2895)
                     /locus_tag="ACSIGJ_001258"
     CDS             complement(2290..2895)
                     /locus_tag="ACSIGJ_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HHQ1330116.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2942..3424)
                     /locus_tag="ACSIGJ_001259"
     CDS             complement(2942..3424)
                     /locus_tag="ACSIGJ_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HHQ1330117.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3628..3725
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3935..5035
                     /gene="gcvT"
                     /locus_tag="ACSIGJ_001260"
     CDS             3935..5035
                     /gene="gcvT"
                     /locus_tag="ACSIGJ_001260"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HHQ1330118.1"
                     /translation="MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVIHGLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLGKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLMRGKEVDVRVLKLPFVRSGQKQFD"
     gene            5182..5721
                     /locus_tag="ACSIGJ_001261"
     CDS             5182..5721
                     /locus_tag="ACSIGJ_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330119.1"
                     /translation="MFFDTEGKSHTAIFMKIVGGNGKIAGEVVIDGEYALDIVRMNDD
                     LSIAVQDNKGRSRASDLEALSQAKSFTLIAFSRYAGTVEVDKVSAVTGICADYRSKGL
                     KVAVADNMRPKQEIVVFRASGVMASNKRKINEAYLDYSASSKLPSAQKQELEQDKNNM
                     IAVHYGRLEELIARGICVR"
     gene            5773..6159
                     /gene="gcvH"
                     /locus_tag="ACSIGJ_001262"
     CDS             5773..6159
                     /gene="gcvH"
                     /locus_tag="ACSIGJ_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HHQ1330120.1"
                     /translation="MSNNIPTELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQSGVVESVKAASDVYAPIAGEVVAVNDDLPGAPETANSDPYGAGW
                     FFKIKPANPADYDGLLTAEQYAGEVD"
     gene            6312..7559
                     /gene="hemA"
                     /locus_tag="ACSIGJ_001263"
     CDS             6312..7559
                     /gene="hemA"
                     /locus_tag="ACSIGJ_001263"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HHQ1330121.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYTLDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRAAQEQESMGAKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKNPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            7806..10112
                     /locus_tag="ACSIGJ_001264"
                     /pseudo
     CDS             7806..10112
                     /locus_tag="ACSIGJ_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753899.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            10241..12208
                     /gene="nosZ"
                     /locus_tag="ACSIGJ_001265"
                     /pseudo
     CDS             10241..12208
                     /gene="nosZ"
                     /locus_tag="ACSIGJ_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-dependent nitrous-oxide reductase"
     gene            12505..12648
                     /locus_tag="ACSIGJ_001266"
                     /pseudo
     CDS             12505..12648
                     /locus_tag="ACSIGJ_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            12681..13736
                     /gene="nosD"
                     /locus_tag="ACSIGJ_001267"
                     /pseudo
     CDS             12681..13736
                     /gene="nosD"
                     /locus_tag="ACSIGJ_001267"
                     /inference="COORDINATES: protein motif:HMM:NF016906.6"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            13794..14690
                     /locus_tag="ACSIGJ_001268"
     CDS             13794..14690
                     /locus_tag="ACSIGJ_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHQ1330122.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVSQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQTAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGPDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIVVMKNGVKVADGSMD
                     ELHVQSGLPLTVNIRLNAPRTLSSRWQPLSDGISYRAQCQAEERMELLGELGSLSDLA
                     YFDIHTPTLDEMYARFLKREDV"
     gene            14687..15517
                     /locus_tag="ACSIGJ_001269"
     CDS             14687..15517
                     /locus_tag="ACSIGJ_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHQ1330123.1"
                     /translation="MNPVWIITGKEARDSLRNRWVLAAVLLLAALALSLGFLGSSPTG
                     SVKVDPLTVTVVSLSSLSIFLIPLIAMLLSYDALIGEIERGTMALLLSYPIWRNQILA
                     GKFVGHLIILALATTAGYGLAGITLQLANGGFDIAVWKPFALLIAASVILGAAFLSMG
                     YLISAKVKERGTAAGISIGVWLFFVVIFDMALLGILVADSKQVITAPVVETVLLFNPT
                     DIYRLLNLTGYENTAMYAGMAGLSGQIGLTVPVLLTAQVLWVIIPLVLAAGIFRKRRI
                     "
     gene            15514..16008
                     /locus_tag="ACSIGJ_001270"
     CDS             15514..16008
                     /locus_tag="ACSIGJ_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HHQ1330124.1"
                     /translation="MKKTLLAIVAVFALSACRQAEEAPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTVKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWIDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDA
                     YIFK"
     gene            complement(16142..17803)
                     /locus_tag="ACSIGJ_001271"
     CDS             complement(16142..17803)
                     /locus_tag="ACSIGJ_001271"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HHQ1330125.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VAEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GVGKDGGPTDSFQPGMELWARQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFIYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGVLIGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENFEEVESFDSGKEAGNYQKLFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSLYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAVCKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIIE
                     ENGSPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(17933..18514)
                     /locus_tag="ACSIGJ_001272"
     CDS             complement(17933..18514)
                     /locus_tag="ACSIGJ_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HHQ1330126.1"
                     /translation="MFNDNPVVYGKIKLQSWKARRDFNIVKQDLDFSCGAASVATLLN
                     NFYGQKLTEEEVLEKLGKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIV
                     YLKYRKDDHFSVLRGIGGNTVLLADPSPGHVSMSRAQFLEAWQTREGNLAGKILAVVP
                     KGRDAGGDKAFFTRSPKRQTEFAVGQVKWWRAY"
BASE COUNT         4513 a         4809 c         5000 g         4271 t
ORIGIN      
        1 ttgagggaat gcgaatttcg acttaacttc ggcacaccgt cccggcagct aaaaatcctg
       61 cgggatcggt gtggaattta gggataatct agtacagccc ctttgattat gccggaattt
      121 aaaatgccgt ctgaatgttc agacggcata gcgtttacag tagtttgaaa acgaaaaaga
      181 cgagggtatg tacgatgaag acgggtgtca ggaaggcgac cgaccacatc atatagccga
      241 agaaagtcgg catcggtacg ccgcgctgtt cggcaatggc cttgaccatg aagttcggtg
      301 cgttgccgat gtaggtcagt gcgcccatga ataccgaacc catagaaacc gccagcagcg
      361 aatgaaacag gggacccgtc attaaggctt gggcatcgcc gcccgccata ttgaaaaaca
      421 cgagataagt gggcgcgtta tccaagaatg ccgacaatat gccgctcatc cagaaataca
      481 tcgtattaat cggatgacct gccgtatcgt gaaccagcga taccaccccg cccagcgcgc
      541 ctgcctcgcc tgctttcaga atgctcagga cggggaagat ggtgatgaag atgccgagga
      601 agagtttgcc cacttcggcg atgggttcaa agttgaattc gttgcctgcg cggacttgtt
      661 tgggcgtgat tgccatagat acggcggtca atgtaatcag gatgacatcg cggacaaggt
      721 tttgcagggc gtaacggctg ccgaggattt caaatcccgg gtgttcgggt ttccaaaggc
      781 cggacattag aaccgcgccg accacgcccg aaagcaggag gaagttccat ttgccgaaga
      841 tggcgatttt ttcgggtttt tcctgttgtg ccggcgtatc ttgtgcaatg ctttcctgtt
      901 tgaagaaacg gttgtcgatg aaatagaagg cggtcaacag gacagcggtg ctaatcagga
      961 cgggggcgaa catatgtttg accgtccaca tgaaatctac gcctttgagg aagccgagga
     1021 agagtggggg gtcgcccaaa ggggtcaggc cgccgccgat gtttgcaacc aggaaaatga
     1081 agaagatgac gatgtgcacg cggcgggtac ggtcttggtt ggctttcagc agcggacgaa
     1141 tcatcagcat tgccgcgccg gtcgttccca tgatagaggc aagtgccgta ccgacggcaa
     1201 gcagggcggt gttgagcttg ggtgtgccgt tcaagtcgcc ccaaaccaaa atgccgcctg
     1261 aaatggtgta cagggcaagc agcagcagga tgaaggggat atattcttca acgagtgcgt
     1321 gtgcgacggt atggataccg gcggacgcgc caaaaaccaa actgaacggg atgaggaaga
     1381 gcaatgtcca aaaggcggtg attttgccgt aatggtgatg ccaggtatgc gaaaaaaaca
     1441 agggacccaa tgcgatagac agcaaaatca gggcaaaggg caggctccac agcaggttta
     1501 ggtttgcgcc gtccgaatct gcggcgtaaa ccgatgcggg gaaaagcatt agtgaaaaca
     1561 ggggtaggtg gcgcatcgtg tttcctcgat tcaagcactg ccttgcgcgg cgcgtgggag
     1621 tgatacaggc accgtgccgc ccggacatag gcggactgtg ctgcctgttt gtttttgaaa
     1681 aaagttttcc gattgattgt aaagtaaata atcaggataa accagtttcc caaaccggaa
     1741 ggcggcggga aggcggattg ctgtgcttgg gaatatatgt tctttttgat aaataatttt
     1801 atttaaaaca aataaatata tgtctttaat aaataatctg tcgaaaacga aaaatgaatt
     1861 tattttaaca tatatttgca atgaaacagg tttgcccccc gtttgtttgc ccttatccct
     1921 ttcagtacgg cattcaagat tcgggcctgc gccacatcca tatggcgaca agggaacaaa
     1981 aaaccgatga aaccgccccg acccaccagc gttggggaaa ctgccaaaac attatcaggc
     2041 aggatgcggt catcatactg atggcgaata ttttggcttt gcgcggcact gcgccgtttt
     2101 gttcccagtt atgaaccatc ggaccgaaat agcggtgccg gtgcagccag cggtgaaagc
     2161 gcggggatgc ctttgcccag caggcggcgg agagcagtac gaacggcgtg gtcggcaaca
     2221 gcggcaaaaa aatgccgatg atacccaaca gcagggaaat gcagccgcag gcaattaaaa
     2281 gataacgtat cattttgaaa tatttttctt attgtgcgga taagggcagg atgtgatacc
     2341 gagttttgcc cagccttcat gtcccatttt ttccagcagg gcgatattgc gttcgaatat
     2401 tgccgaagcg tcgggaaagg cttgtgcggc tttggcaatg ctgtcttcgc ggatgaggtg
     2461 cagcgtcgga tagggagaac ggttggtgta gttgccgatg tcgtcgggtt cggtattctg
     2521 aaagcggaat tggggatgga acggtgcgat ttggacgatg ccttccaagc cgttttcgat
     2581 aacggcggca tcggcaatgt cgagcatatc gttgaatacg tcgaaatcgg ggaatagggt
     2641 cgggtgaacc agcagggtgg tttccagttc ggtggcgggt gtattgccca gtcgctgcag
     2701 ttcttcgtcc aagtcttcca aaaaactgtc aaggtgtttg gcttcgctga ttgcgatgcg
     2761 gacaaggttt ttaacgtggg gggctttggc aaagggacac aggttcagac cgatgacggc
     2821 tttttccaac cattgtccgg tgtgttcggc aacagcatct ttattttcgg aagtattgat
     2881 attcattatt gtcatgtaaa tgtgtttgca gattgcacgt gcgggaaaat cgggaagggc
     2941 actattcctt cagcaggtgg ttgagcggca gggaggtggt gtgtttgatt tcttttaaaa
     3001 caaagctcga ttgcgcatct tgtacgccgt ggtgggacag gagcgtatcc aaaacaaaat
     3061 gggaaaacgc gttcatatcg gtaaaaaacg cctgaagcag gtagtcggtt tcccctgtca
     3121 gggcgaagca gctcaagact tccggccatt ttcggaccga tgcggcaaag tcttcccgcg
     3181 cgtcttttgc tttgcggatg gaaacgcgga taaatgcctg aagccccagg ttgacggatt
     3241 ccggagacag cagcgcggca tattggcgga cgatgccggc atcttccaac tgcttcagac
     3301 ggcgcaggca cggagaaggc gaaagtgcga cacgttcgga cagttcgaca ttggtcagcc
     3361 tgccgttttc ctggagaacc tgtaagattt taatatcggt tttgtctaaa gtgagttggg
     3421 gcatatttgc gttccgtttt aaggaattcg gattgtctgt ccgtatgttt gcggcaatcc
     3481 gcacagatgg agaccatatt aacatataaa aagttatacc gtcatccggg acaaattttg
     3541 ttttcggaaa atcatgtgaa aacagaggcg gtcggtttgc atctctttta agacggcttg
     3601 cccaaaccgc cgattcaaga cataatcggg aaatgtgcag gagagtgtta cacccaacta
     3661 caatgtaacc accgaaggcg cagacaccct taaatcgctc aggtatcagg gactgcacat
     3721 tgaaacaaac aatctggaga gcggcgttgg aataacgtcc accgaagggg agaaggccgt
     3781 ctgaaccacc attcagacaa ccgcgcaaag cagtgagcag actggtttgc catcatgcgg
     3841 atacggccga aaatctcagg ttcaaggaca gatagggtca tccgcgcatt ttgtgcggac
     3901 ggcatctgaa caaaaaatcc ggagaaactt gagaatgatt actctgaaaa ccaccccatt
     3961 tcatcaagcc catcaagatg caggcgcgaa gccggtagat tttgccggct gggagctgcc
     4021 catccattat ggttcgcaaa tcgccgaaca cgaagccgtg cgcaccgacg ccggtatgtt
     4081 tgacgtatcc catatgcttg ttaccgacgt agcaggcgca aatgccaaag cctttttccg
     4141 caaattgatt gccaacgatg tcgccaagct cgcttttgtc ggcaaagccc tttattccgc
     4201 tttgctcaac gacaacggcg gcgtgattga cgacttaatc gtttaccgca ccaacgaagc
     4261 cgaaacccaa taccgcatcg tgtccaacgg agcgactcgc gaaaaagaca cggcgcaatt
     4321 ccacaaagtc ggacaagagt tcggcgttgc ctttaatccg cgctacgacc tcggcatgct
     4381 cgccgtacaa ggccctaaag ccatcgaaaa actcctgacc gtcaaacctg aatgggcgga
     4441 tgtgattcac ggcctcaaac cgttccaagg tgcagacttg ggcaacgatt ggtttgtcgc
     4501 ccgcaccggc tataccggcg aagacggcgt cgaagtgatt ctgccgggca ctgaagccgt
     4561 cgcattcttc aaagccctgc aagcagcagg cgtacaacct tgcggtctcg gcgcgcgcga
     4621 caccctgcgc atggaagccg gcatgaacct ctacggcaac gatatggacg acgataccag
     4681 cccgctcgaa gcaggcatgg gttggaccgt tgatttgaaa gacgaaagcc gcgattttgt
     4741 cggcaaagct gccttgttgg cattgaaaga aaaaggcgtt gccgtcaaac aggtcggttt
     4801 gctgctcggc aaaggcggca tcctgcgcgc gcatatggaa gtgttgaccg acaaaggcaa
     4861 aggcgaaacc accagcggcg tattctcgcc cagcctgaaa caatccatcg ccatcgcccg
     4921 cgtaccgaaa gactttgacg gcgataccgc taaagtgctg atgcgtggca aggaagtgga
     4981 cgtgcgcgta ctgaagctgc cgttcgtccg cagcggtcaa aaacagtttg attaatatcg
     5041 gttcagacgg catttgtttg aattaagccg tctgaaagca ggttttaagg aattgttatt
     5101 tcctatgaaa gcaaattttg gcagtttatt gttgttattg atgttatcgg cttgcgcttc
     5161 gtcgccggcg agcgcggatt gatgtttttc gatacagaag gaaaatccca tactgcaata
     5221 tttatgaaaa tcgttggcgg caatggaaag attgccggcg aggtggtgat agacggggaa
     5281 tatgctcttg atattgtccg catgaatgat gatttgagta ttgccgtgca agataataag
     5341 ggtagaagcc gtgcttccga cctggaagca ttgtcgcaag cgaaatcatt taccctaatt
     5401 gcgttcagcc gttatgcggg cacagttgaa gtggataaag tatcggctgt tacaggcatt
     5461 tgtgcagatt accgcagcaa aggcctgaag gttgccgttg cagacaatat gaggcccaaa
     5521 caagaaatcg ttgtattccg tgcaagcggc gtaatggcaa gtaataagcg caagataaac
     5581 gaagcatatt tggattattc tgcatcctct aaactgcctt ccgcccaaaa gcaagaattg
     5641 gaacaagata aaaacaatat gattgccgtc cattacggac gcttggaaga attaatcgca
     5701 cgaggtatct gcgtacgcta agttgtgcag atgccgtctg aaaataaccc cctaccaatg
     5761 gagaatcaaa ccatgagcaa caacatccca acagaactga aatacgttgc cagccatgaa
     5821 tggctgcgcc ttgaagaaga cggtaccatt accgtcggta ttacccatca cgcgcaagag
     5881 ctgttgggcg acatcgtgtt tgtcgagctg cccgaagtcg gtgcgaacct cgccgccgaa
     5941 gaacagtccg gtgtggttga gtctgtaaaa gccgcatccg acgtgtatgc gccgattgcg
     6001 ggcgaagtcg ttgccgtcaa cgatgacttg ccaggcgcgc cggaaaccgc caacagcgac
     6061 ccttacggcg caggctggtt cttcaaaatc aaacctgcca atcctgccga ttacgacggt
     6121 ctgctgactg ccgaacaata cgcgggcgaa gtggattaaa ccgcccgttt gtccgacggc
     6181 aaccgtcgga caaacggaaa ctgcaccttc agacggcatt tctgcggtcg gaggtgcagt
     6241 ttttttgtcc gtgttttaag gatgcagtta ggctataata acggtctata tccatcttca
     6301 ccgatttttt catgcaactt accgctgtcg gactcaatca tcaaaccgca cctttaagca
     6361 tacgggaaaa gctggcgttt gccgccgccg ccctgccaga agccgtccgc aatcttgccc
     6421 gaagcaatgc ggcaacggag gcggtaatcc tttctacctg caaccgcacc gagctttact
     6481 gcgtcggcga ttcggaagaa atcatccgat ggcttgccga ttaccacagt ttgccgattg
     6541 aagaaatccg tccgtatctg tacacgctgg atatgcagga aaccgtgcgc cacgccttcc
     6601 gcgttgcctg cggcttggat tcgatggttt tgggcgagcc gcagattttg gggcagatta
     6661 aagatgcggt gcgtgcggct caagaacagg aaagtatggg ggcaaaactc aatgccctgt
     6721 tccaaaaaac cttttccgtt gctaaagaag tccgtaccga taccgctgtc ggcgaaaatt
     6781 cggtttcgat ggcttccgcg tccgtcaagt tggcggaaca gatttttccc gacatcggcg
     6841 atttgaacgt attgtttatc ggcgcaggcg aaatgattga gctggttgcc acttattttg
     6901 ccgccaaaaa tccccggctg atgacggttg ccaaccggac gctggcgcgt gcacaggagt
     6961 tgtgcgacaa gctcggtgtt aacgcggaac cgtgcctgct gtccgatctg cctgccattc
     7021 tgcacgatta cgacgtggtg gtttcttcaa cggcgagcca gcttccgata gtcggcaaag
     7081 gcatggtcga acgcgcattg aaacagcgtc agagtatgcc gttgttcatg cttgacttgg
     7141 ccgtgccgcg cgatattgaa gcggaagtcg gcgatttgaa cgatgcgtat ctttatacgg
     7201 tggacgatat ggtcaacatc gtccaaagcg gcaaggaggc aaggcagaag gccgccgccg
     7261 ccgccgaaac gctggtgtcc gaaaaggttg ccgaatttgt caggcagcag cagggcaggc
     7321 agagcgttcc gctgattaag gccttgcggg acgagggcga gaaagcgcgc aagcaggtgt
     7381 tggaaaatgc gatgaaacag cttgccaaag gcgcaacggc ggaagaggtt ttggaacggc
     7441 tgtccgtcca actgaccaac aagctgctgc attcgccaac tcaaaccttg aataaggcgg
     7501 gggaagaaga taaagatttg gttcatgccg tcgcgcagat ttatcatttg gacaaataac
     7561 ggtgcgccgg aaaaggatgc cgtctgaagg ggtttcagac ggcatatttt tgtgccgcct
     7621 gacaacatcg tgaaatccca cattatatcg atgtaattac aaagtattat cctatatgcg
     7681 gattggtttt aagatttgta aatttgattt gcatcaaaaa tcgccgatag atgattcata
     7741 taatatcaat attaaagagt atcggtatat cggggatagc tatgtcctgt ttttcaatcc
     7801 ggcgtatgtc tgcgtttcgg gcgcggataa cggcgttttt taccgccttt gtctttttga
     7861 cggcggcact gcccgcttat gcggagcgtc tgcctgattt tctggcgaaa atacagcctt
     7921 cggaaatttt tccgggtgcg gatcgttacg gcaagccgga aggcaagcct atggttgccc
     7981 gcgtttacaa aggcgatgag cagctcggtt tggtttatat cacgaccgat gcggtcaata
     8041 cgcgcggtta ttcgagcaaa ccgatcgata cgctgatggc tttggcaaac gacggcacga
     8101 tagccggggc gaaactggtc gatcatcacg aaccgattat gctgatcggt atcccgcaat
     8161 cgcgtgtcga taagttcatc gacaaatata tcggtctgaa ttttattaaa aatccgccga
     8221 ccccgggcgt ggcgccgggc gacatcatca gcggtgcgac tgttacactg atggtggtta
     8281 acgacagcat ccagcgttcg tacaaggtca ttgccaacca ataccgtctg ggttcggaca
     8341 aggcccttca gacggcatcc gcttccgatg ttcgggaagc cgcgcctgcg tcagaaaccc
     8401 gtccgcgccg tatggcaaat cccgacaagc aggatatttt gtcttgggac gaacttttga
     8461 aacaaaaggc cgtcggccat ctgcatatca cgctcgatca aatcaacaaa ctgtttgaga
     8521 aaggcggcaa ggccggcgtg gccgatcacg ccgaacaggg cgatcctgac gataccttta
     8581 ttgatttgta tgttgccttg gtcagccagc cttccatcgg taaaagcctg ctgggtaagg
     8641 acggctgggc gcatctgcaa aaacggctga aacccgggca gcaggcggtt ttggttgccg
     8701 gagagggccg ttattcttgg aaaggttcgg gctatgtgcg cggcggtatt ttcgaccgta
     8761 tcgagatgat tcagggggag aacagcttcc gttttaccga tgcccaacac gaacgcgtcg
     8821 tcgagctgtc tgccgccgat gcgccgcgtt ttaaagaagt ttcttggttt accatccctg
     8881 aaggcgtagc gtttgacggt gcggagccgt ggcggctgta attgatggtt cagcgcgtat
     8941 tgagtgtgaa cgataaagcc ttcgttaccg ccgatttgga ttacgaactg ccccaagctt
     9001 attacgtcga tgatcctaaa gcgccgcccg tagaaatcag cgcgcctgtc gaagcagtac
     9061 ctgccgcagc atccgatacc gcttcagacg gcattgccga agatgcgtca gctgaaaacg
     9121 gtgtatccaa ccaattatgg aaacagattt ggaaagcgaa acaggggcag attgtcgtcg
     9181 tcggcattgc gctgaccatc ctgcttttgg tattcctgtt ccaagattgg attgtgcgtt
     9241 acgaaaaatg gtacgaccgc ttccgctttg ctttcctaac ttttacgctc ttctatatcg
     9301 gctggtatgc acaggcgcag ctttcggtcg tcaatacgct gacgctgttt tccgccatcc
     9361 ttaccgaatt tcattgggaa ttcttcctga tggatccgat tgtgttcatc ctgtggctgt
     9421 tcacggctgc aaccatgctg ctgtggaaca gggggacgtt ttgcggttgg ttgtgtccgt
     9481 tcggctcatt acaggaattg actaaccgca ttgccaaaaa actgggtgtg aaacagatta
     9541 ccgtgccgca tatgctgcat acccgtttga acgtcataaa atacctgatt ttcttcggtt
     9601 tttttgcaat ttcgctgtat gatttgggta cggcggaaaa atttgccgaa gtcgaaccgt
     9661 tcaaaactgc gattatcttg aaattcatgt gcgattggtg gtttgtcgcc tttgccgtgg
     9721 cacttttgat tgcggggctg tttatcgaac gcttcttctg ccgttatctg tgtcctttgg
     9781 gtgcgggcat cgcgctgccg ggcagattcc gcgtgttcga ctggctgcgc cgctacaaaa
     9841 tgtgcggcaa cccctgccaa atctgtacgc acgaatgccc cgttcaggca atcgcgcccg
     9901 agggcgacat ccatccgaac gaatgtatcc agtgcctgca ctgccaagtg atgtaccacc
     9961 acgatacgcg ctgtccgcaa gttgtggcgg aaaataagaa aaaacaaaaa caggctgctg
    10021 ccaaatcagg ggaattggaa aatgtctcta agcagccgca ggaacaggtc gtccgttttg
    10081 tcaaacccga aacggcgcaa tctgaaaaat aagcatccgg gggaagcctg aagccaaagt
    10141 gtcaggcagg cagggtttcc ccggaaggat aataaaattt ttttcccatc ggcttgccgt
    10201 ccgaaaaccg gtttgccgta ttttatccaa ggagtgttgt atgtcagacg aaaaattaga
    10261 acaaaacggc ttgagccgtc gttcgttctt aggtacggcc gccgcttccg gtgcaggtat
    10321 tgccggtgcg ggcctgttgg gtttggcggg ttgctctaaa gacggcgaac aggctgccgc
    10381 taacgcttcc ggcgcggctc ccgtcgccaa ggcgcaaggg gaatccaaac ccggccaact
    10441 gtcttccgaa gtcggtccgg gcgaactcga tcaatattac ggtttccttt ccggcggcca
    10501 gtccggcgag atgcgcctga tcggtctgcc ttctatgcgc gaactgatgc gtatccccgt
    10561 gttcaatatg gacagcgcga ccggttgggg acgcaccaat gagagcttga aagtcctcaa
    10621 cggcaatatt accgaagaaa cccgtaaatt cttaaaagac ggcggcctgc gctgctaccc
    10681 caacggcgac ttgcaccacc cgcacctgtc gtttaccgac caaacttatg acggccgcta
    10741 tgcctatgcc aacgacaagg caaacaaccg cgtctgccgc gtgcgtttgg atgtgatgaa
    10801 ggccgacaaa atcatcgaca tccctaatga ttcaggtatt cacggtctgc gtccgcaacg
    10861 ttatccgaaa accggttatg tttttgccaa cggcgaacac attacccctg tcagcggtgt
    10921 aggcaaactg gatgatgcga aaacctggaa tgcagtgtac accgccatcg acggcgaaac
    10981 catggagatc gcatggcaag tattggttga cggtaacctg gacaacggcg atgccgacta
    11041 tcaaggcaaa tattcttttg ccacctgcta caactccgag cgcgcgctga ccgtacaagg
    11101 tgcgtcttcc aacgagcagg actggtgcgt cgttttcgac ctgaaagcca tcgaagaagg
    11161 catcaaagcg ggcgacttca aagaagttaa cggtgtgaaa atgctggacg gccgcgctga
    11221 ggccaaatcc aaatacacgc gttatatccc tgtgccgaac tctcctcacg gctgtaacgc
    11281 aagccctgac ggtaaataca tcatgcccaa cggtaaactg cctccaaccg ttaccgtatt
    11341 ggatgtcagc aaactggacg atttgttcgc cggcaaaatc aaagagcgcg atgtggtcgt
    11401 agccgaaccg caactgggtc tcggcccgtt acacactgca ttcgacggtc gcggcaatgc
    11461 ttatacgaca ttgtttatcg acagccaaat ggtgaaatgg aatattgacg atgcgatcaa
    11521 agcctacaaa ggcgagaaaa tcgatccgat caaacaaaaa ctcgacgttc actatcaacc
    11581 gggtcacaac catacgacca tgggcgaaac caaagaagcc gacggtcaat ggctggtgtc
    11641 cttgaacaaa ttctccaaag accgcttctt gaatgccggt cctttgaaac ccgagtgcga
    11701 ccaattgatc gacatctccg gcgacgaaat gcgtctggta cacgacaacc cgacttttgc
    11761 cgaaccgcac gacttgtgtt tggttgccgc gtccaaactg aacccgggca aaacttggga
    11821 ccgcaaagac ccgtggttct agcaggatgc tttggaacag gcgaaaaaag acggtgtcga
    11881 gctggaaaaa gccgccaaag ccgtacgcga aggcaacaaa gtacgcgtgt atatgacggc
    11941 cgtcgcgcct gcttacagta tcccgcagtt tgaagtgaac caaggcgacg aagtaaccgt
    12001 ttacgttacc aacgtcgaaa ccatcgagga cctgacccac ggcttcactt tggaaggctg
    12061 cggcatcgct atggaaatcg gcccgcaagc cacttcttcc gtaaccttca aagccgtccg
    12121 cccgggtgtg cactggtact actgccaatg gttctgccac gcattgcata tggaaatgtc
    12181 cggtcagatg attgttcatc ccaaataatg cggcccgacg gctgaatatg gagaatggcg
    12241 tctgaaggca acaggtcttc agacggcatt tatagccaaa ccgcttgccg taccgtttca
    12301 ggcggtttcg ctataaaaac aggcacattt gaatgatgct ttattttgtt cggccatacc
    12361 gaacgaaaca gagggacact atgcacacat ccgcgctccg tatatggctg aaggccgtgc
    12421 tgatacttgc cgccggcagc atttttcaga cggcatcggc agccgtcgtc cacgtttccc
    12481 cgcaagacaa ccttgccgaa atttttgccc gcgcccgggc gggcgatacg atcaaactgg
    12541 cttccggcgt atatcaaacc aaactttata tcgacaaacc gattacgatt gaagggcctg
    12601 ccgaccgttc cgcaaccatc gaaggggaca agagcgggcg taccatagcc gtacacgcgc
    12661 cggacgtaac gctccgcaac ctgaccgtta cccgttccgg tatgagcctg ccagcaatgg
    12721 atgccggtat ttatctcgaa aaagccgccc cgcgccccct ggttgaacac aacaatattt
    12781 ttgataattc gttcggcgta taccttcatg gttctgccga tgcgatggtg cgcgagaata
    12841 aaatcgtcgg cgatgcgacc ttgcgcgtga atgagcgcgg caatggcgtt accgtttgga
    12901 acgcgcccgg cgcgcaggtc gtcggcaacg acatttccaa agggcgggac ggcatttttt
    12961 ccaataccag cacgcacaac acctataaaa acaaccgctt cagcgacctg cgtttcgccg
    13021 tccactatat gtacaccaac gacagcgaag tcagcggcaa tatttccgtg ggcaacaata
    13081 tgggctatgt gctgatgttt tccgaacggc tcaaagtgtt cgacaatatc gccgtcggca
    13141 gccgcgatta gggcatcatg ctcaactatg tcaactattc cgatattcac gacaatatta
    13201 tcaacaaagc gggcaagtgc gtttttgcct acaatgccaa ctacgataaa ctgtccgcca
    13261 atcattttga aaactgccaa atcggcatgc actttaccgc cgccatcgaa ggcacgtccc
    13321 tgcacgacaa ttcctttatc aacaacggaa gccaggtcaa atatgtcagt acgcgctttc
    13381 tcgactggag cgagggcgga cacggcaact actggagcga caacagcccg ttcgatttga
    13441 acggcgacgg cttcggagac agcgcgtacc gtcccgacgg catcatcgac caaatcatct
    13501 ggcgcgcgcc cgtatcgcgc ctcttgatga acagtcccgc aatcagcatc gtcaaatggg
    13561 cgcaggcgca gtttcccgcc gttctgcccg gcggcgtggt ggacagcaaa ccgctgatga
    13621 agccttatgc ccccaaaatt caaacccgtt atcaggcgat gaaggacgag ttgctcaaag
    13681 aagccgaaac gcggcagtcg gaacggggca gggcggaaaa cggttctttg aactagtctg
    13741 cttcagacgg cacccggatt caaatgccgt ctgaaaacac aaaaggaaca accatgacca
    13801 cacatcatgt cgaattgagg aaggtaacca aacggttcgg ggcgcaaaaa gccgtcagcc
    13861 aagtcgattt ggttttgaag gcaggagaaa gcgtcgggct tgccggacac aacggcgcgg
    13921 gcaagtccac catcatgaag ctgatactcg ggctgattac cccgaccgaa ggcgaagtga
    13981 tgcttttggg cgaacgtacc ggcagcaaag cgggggcgcg gcttcgcagc caaatcggct
    14041 acctgcccga aaccgttgcg ctgcaccctt cgctgaccgg catcgaaacg ctggattttt
    14101 atgccaaact taaaaaacag ccgctcacgc agaaccgggg gctgcttgag cgcgtcggca
    14161 tttcccagac cgcacaccgc cgcgtcggca cttattctaa agggatgcgc caacgccttg
    14221 ccttggcaca agccctgctg ggcgagccca aagtcctgct gtttgacgaa ccgacaaccg
    14281 gccccgaccc tgcatcacga caaatgtttt acgaagtcgt gcgcgaactc aacgggcgcg
    14341 gcgcgaccgt attgctcagt acccacgccc ttgccgagtt ggacggacac gccgaccgca
    14401 ttgtcgtgat gaaaaacggc gttaaggttg ccgacggcag tatggacgaa ttgcacgtcc
    14461 aaagcgggct gccgctgacc gtcaatatcc gcctcaatgc gccgcgcacc ctcagcagcc
    14521 gctggcagcc gctttcagac ggcatttcct accgggcgca atgtcaggcc gaagaacgta
    14581 tggaactgtt gggcgaattg ggcagcctgt ccgaccttgc ctatttcgac atccacacgc
    14641 cgacgcttga cgaaatgtac gcacggttct tgaaaaggga ggacgtatga accccgtttg
    14701 gattattacc ggcaaagaag cccgcgacag cctgcgtaac cgctgggtgc ttgccgccgt
    14761 cctcctgctt gccgcactcg ccctttcttt gggtttcctc ggcagctcgc ccaccggttc
    14821 ggtcaaggtc gatccgctga ccgttaccgt cgtcagcctg tcgagcctgt ctattttcct
    14881 gattccgctg attgcgatgc tgctttctta tgacgcactg atcggcgaaa tcgaacgcgg
    14941 tacgatggcg ttgctgttga gttatcccat ttggcgcaac caaatccttg ccggcaagtt
    15001 tgtcggacac ctcatcatcc tcgcccttgc caccacggca ggttacggat tggcaggcat
    15061 tacgctgcaa cttgccaacg gcggtttcga catcgccgtt tggaaaccct ttgcgctgtt
    15121 gattgccgcc agcgtgattt taggtgcggc atttctgtct atgggctact tgattagtgc
    15181 aaaggtcaaa gagcggggga cggcggccgg tatttccatc ggcgtatggt tgtttttcgt
    15241 cgtcatcttc gatatggcgc ttttgggtat tctggttgcc gactcgaaac aggtcatcac
    15301 cgcgcccgtc gttgaaacgg tgctgctgtt caatcccacc gacatttacc gcctgctcaa
    15361 cctgaccggt tacgaaaata cggctatgta tgcgggtatg gcgggtttga gcggacaaat
    15421 cggcctgacc gtccccgttt tgctgaccgc gcaggtttta tgggttatca ttccgcttgt
    15481 tttggcagcc ggaattttta gaaagcgacg aatatgaaga aaaccctgtt ggcaattgtt
    15541 gccgttttcg ccttaagtgc ctgccggcag gcggaagagg caccgccgcc tttaccccgg
    15601 cagattagcg accgttcggt cggacactat tgcagtatga acctgaccga acacaacggc
    15661 cccaaagccc agattttttt gaacggcaaa cccgatcagc ccgtttggtt ctccaccgtc
    15721 aagcagatgt tcggctatac caagctgccc gaagagccca aaggcatccg cgtgatttac
    15781 gttaccgata tgggcaatgt taccgattgg acgaatccta atgccgacac ggagtggata
    15841 gatgcgaaaa aagcctttta cgtcatcgac agcggcttta tcggcggtat gggcgcggaa
    15901 gacgcgctgc cgttcggcaa caaggagcag gctgaaaaat ttgcaaagga taaaggcggc
    15961 aaggtcgtcg gttttgacga tatgcccgat gcttacattt tcaagtaaat ccaaacagaa
    16021 atgccgtctg aacgttgaag ccaaggttca gacggcattt tttgtcatag gttcaaacgg
    16081 ggcaggttcg aacaactgcc ccattcctta ttcctgaccg ggcgtaacgg cggaatgagg
    16141 tttacatccc gccgtaattc ggtccgcttg cgccttcggg gcaaatccaa gtgatgtttt
    16201 gcgtcgggtc tttgatgtcg caggttttgc agtgcacgca gtttgccgcg ttgatttgca
    16261 ggcgcggact gccgttttct tcgatgattt catatacgcc ggccggacaa tagcgcgttt
    16321 cgggagaggc gtattcttta tagttcacgt ctatcatcgt ttgcggattg ttcagcacca
    16381 aatggtcggg ctggttttct tcgtgcgcga gattggcaag gaagacgctg ctcaagcggt
    16441 caaaggtcaa cacaccgtcg ggtttcggat aatcaatcgg cttgcacacg gcggcttttt
    16501 tgagctgctc gttgtctttg ccgtgatgtt tcaaggtcca cggggctttg cctctgaaaa
    16561 tcatctggtc gatgccggta taaagcgagc cgaggtaaac gccccatttg aatgacggac
    16621 ggacattgcg cgcggcataa agctcttgat acagccagct ttgttcaaac agtttttgat
    16681 agtttcccgc ttctttgccg ctgtcgaagc tttccacttc ttcaaagttt tccaacaagg
    16741 ggaacacggc ttcggcggcg agcatggcgg atttcatcgc ggtatgaatg cctttgatgc
    16801 gcggcatatt gaggaatccc gccgcatcgc cgattaaaac gccccccttg aacgagagct
    16861 tcggcaggct ttgcaaaccg ccttcaatca gcgaacgcgc gccgtaagca atgcggcggc
    16921 cgccttcaaa ggttttgcgg atttcgggat gggttttgaa acgttggaac tcttcaaacg
    16981 gcgacagata cgggttttga tagtccaaac cgaccacgaa gccgacggcg actttgttgt
    17041 cgtcgaaatg gtaaataaac gcaccgccgt aggttttgct gtccagcggc cagcctgcgc
    17101 tatgcaccac caaaccgggc tgatgctgtt cagacggcac ttcccaaact tctttgatgc
    17161 ccaagccgta agtttgcggc tggctgtttt ggtcgagttg gaaacgttcg atgatttgct
    17221 tggaaagcga acctcggcaa ccttcggcaa acagggtttg ccgcgcccaa agctccatgc
    17281 cgggctggaa actgtcggtc ggcccgccgt ctttgcctac acccatattg ccggtcgcaa
    17341 tacctttgac cgagccgtct tcgtgataca gcacttcggc ggcggcaaag cccggataga
    17401 tttccacgcc catattttcc gcctgctccg ccaaccagcg cacgacttcg cctaagctga
    17461 cgatgtagtt gccgtgattg tcgaaattcg gggtaatagg cagattgaac gcttttttct
    17521 ccgtcaggaa caacaccttg tcctgagtta ccgtgcgcgt cagcggcgcg cctttttctt
    17581 tccagtcggg aatcagctca ttcagcgcaa tcggatcgat aaccgcgccc gccagcgaat
    17641 gcgcccccgc ctccgaaccc ttctccgcca cgcaaacgct gatttcgcgc ccgttctgtt
    17701 cggcaagctg cttgagtttg atggcggcgg acaaccccga cgggcctgcg ccgacaatca
    17761 cgacatcgta ttgcatactg tcgcgggtga tggattctgt catggcggtt cctgtgtatt
    17821 tattattgaa ttgcaaatcc gtaattatac aacgggaaca tatagttacc aaatacaaca
    17881 aaggtcgtct gaaaaccata ttttcggttt tcagacgacc tttgtcgaaa tttcaataag
    17941 cacgccacca ttttacctgt ccgaccgcaa actccgtctg acgtttcgga ctgcgtgtga
    18001 aaaacgcctt atccccaccg gcatccctcc ctttcggcac gaccgccaaa attttgcctg
    18061 ccaaatttcc ctcacgggtt tgccaagcct ccaaaaactg cgccctgctc atcgaaacat
    18121 gacccggcga cgggtcggca agcaaaaccg tattgccgcc tatgccgcgc aataccgaaa
    18181 aatggtcgtc tttgcggtat ttcagataca cgatgacggg gattttcaac tgcgcgagct
    18241 gctcgaaaga cagggcatag cccttcgcct caaaacccaa atcgggcata atgcgccgca
    18301 tatcctcaaa cgacgcgcgc atctgctcct tacccagttt ttccaacact tcttcttccg
    18361 tcagcttttg cccgtaaaaa ttgttcaaaa gcgtcgccac cgaagccgcc ccgcaggaaa
    18421 aatccaaatc ctgctttaca atattgaaat cccgccgcgc tttccaactc tgcaatttga
    18481 tttttccgta aacaacagga ttatcgttaa acatcggtgc agcattcaaa cgataagaca
    18541 agggtctgta ctagattagc agatatgtta ccctcgaaat atgaagataa cac
//
LOCUS       DBJGGI010000036        18547 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.036, whole genome shotgun sequence.
ACCESSION   DBJGGI010000036 DBJGGI010000000
VERSION     DBJGGI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18547
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.036"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(467..859)
                     /gene="rpsI"
                     /locus_tag="ACSIGJ_001273"
     CDS             complement(467..859)
                     /gene="rpsI"
                     /locus_tag="ACSIGJ_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HHQ1330127.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLTKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESLDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(872..1303)
                     /gene="rplM"
                     /locus_tag="ACSIGJ_001274"
     CDS             complement(872..1303)
                     /gene="rplM"
                     /locus_tag="ACSIGJ_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HHQ1330128.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVATEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQDQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(1481..1660)
                     /gene="ssrS"
                     /locus_tag="ACSIGJ_001275"
     ncRNA           complement(1481..1660)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACSIGJ_001275"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1666..1971)
                     /locus_tag="ACSIGJ_001276"
     CDS             complement(1666..1971)
                     /locus_tag="ACSIGJ_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HHQ1330129.1"
                     /translation="MNIEQVYIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKITDMDNACQKALSRLGQE"
     gene            complement(1982..2311)
                     /locus_tag="ACSIGJ_001277"
     CDS             complement(1982..2311)
                     /locus_tag="ACSIGJ_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330130.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELERAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENARYRALLEQSREKISALAARLP
                     QRQETQQ"
     gene            complement(2365..3354)
                     /locus_tag="ACSIGJ_001278"
     CDS             complement(2365..3354)
                     /locus_tag="ACSIGJ_001278"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HHQ1330131.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLAEALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGSVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(3477..6179)
                     /gene="ppc"
                     /locus_tag="ACSIGJ_001279"
     CDS             complement(3477..6179)
                     /gene="ppc"
                     /locus_tag="ACSIGJ_001279"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HHQ1330132.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDERQTYDLTLACGLFAQILNIAEDVHHERRRQIHEDAGHNA
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLNFNRRIRALL
                     PQRERCTNADALARLRREIDTVLLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVRVPNILKIGGWIGGDRDGNPFVSGETLRFAFRRHADAVFR
                     FYRSELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARSLGLGMGCKFGFLEPYASAQKFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELGHQRPLYSP
                     FITYSDHTRRELAIFNEARKIKDEFGEDAVTQSIISNCEQPGDLLALALLLKESGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLHQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEGSPETLAALRGHAQNNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAKVIFGMIKEEYRRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            6222..7115
                     /locus_tag="ACSIGJ_001280"
     CDS             6222..7115
                     /locus_tag="ACSIGJ_001280"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HHQ1330133.1"
                     /translation="MPHPCQTVHTLTTHVPKCRLKLNAARRRRYNRPQLFFPNIIMTA
                     TEHDNDDALLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAETLAGRLKRINHTVDVRAVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAAYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVLSPLVGIVGCTQAAEALKILLEAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPVCGAAR"
     gene            complement(7515..7739)
                     /locus_tag="ACSIGJ_001281"
     CDS             complement(7515..7739)
                     /locus_tag="ACSIGJ_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HHQ1330134.1"
                     /translation="MDAVQELERRIVELEIQTALQEDVISGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(7740..9128)
                     /locus_tag="ACSIGJ_001282"
     CDS             complement(7740..9128)
                     /locus_tag="ACSIGJ_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HHQ1330135.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNVIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLVAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCTGL
                     GFSPLATAAIVGTAGALGDAGSPASDSTLGPTMGLNADGRHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(9214..10035)
                     /gene="prmC"
                     /locus_tag="ACSIGJ_001283"
     CDS             complement(9214..10035)
                     /gene="prmC"
                     /locus_tag="ACSIGJ_001283"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HHQ1330136.1"
                     /translation="MTFDEWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEI
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPNVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSERQWDIIVSNPPYIENGDKHLSQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVEILPDLAGLDRVTLGKYMKHLK"
     gene            complement(10452..11306)
                     /locus_tag="ACSIGJ_001284"
     CDS             complement(10452..11306)
                     /locus_tag="ACSIGJ_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HHQ1330137.1"
                     /translation="MKLNAKLKALLASAAIAVGLTACGGGSGDAQSSQSSGAATVAAI
                     KEKGVIRIGVFGDKPPFGYVDANGKNQGFDVEIAKDLAKDLLGSPDKVEFVLTEAANR
                     VEYVRSGKVDLILANFTQTPERAEAVDFADPYMKVALGVVSPKNKPITDMAQLKDQTL
                     LVNKGTTADAFFTKSHPEVKLLKFDQNTETFDALKDGRGVALAHDNALLWAWAKENPN
                     FEVAIGNLGPAEFIAPAVQKGNADLLNWVNGEIAAMKKDGRLKAAYEKTLLPVYGEKV
                     KPEALLAE"
     gene            complement(11419..12150)
                     /locus_tag="ACSIGJ_001285"
     CDS             complement(11419..12150)
                     /locus_tag="ACSIGJ_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HHQ1330138.1"
                     /translation="MALLSIRKLHKQYGSVTAIQSLDLDLEKGEVIVLLGPSGCGKST
                     LLRCVNGLEPHQGGSIVMDGVGEFGKDVSWQTARQKVGMVFQSYELFAHMTVIENILL
                     GPVKVQNRDRAEAEAQAGKLLERVGLLDRKNAYPRELSGGQKQRIAIVRALCLNPEVI
                     LLDEITAALDPEMVREVLEVVLELAREGMSMLIVTHEMGFARKVADRIVFMDKGGIVE
                     SSDPETFFSAPKSERARQFLAGMDY"
     gene            complement(12152..12820)
                     /locus_tag="ACSIGJ_001286"
     CDS             complement(12152..12820)
                     /locus_tag="ACSIGJ_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HHQ1330139.1"
                     /translation="MAFEWLFEGKNAARLGEGLLLTAQISLISVAASCVLGTLFGLVL
                     RSRNRLVRFVGRFYLETIRIVPILVWLFGLYFGLSVWTGIHIGGFWVCVWVFSLWGVA
                     EMGDLVRGALESIEKHQVESGLAPGLSRGQVFRCIELPQSIRRVLPGAVNLFTRMIKT
                     SSLAWLIGVIEVVKVGQQIIENSLLTQPNASFWVYGLIFMLYFFCCWPLSLLAAKLEQ
                     KWEH"
     gene            complement(12810..13469)
                     /locus_tag="ACSIGJ_001287"
     CDS             complement(12810..13469)
                     /locus_tag="ACSIGJ_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HHQ1330140.1"
                     /translation="MNWPYLIDAVPKFADAAKLTLELSVYGVVLSLLFDLPVAVVTAY
                     RIRPFYALARAYIELSRNTPLLIQLFFLYYGLPKMGIKWDGFTCGVIALVFLGASYMA
                     EAVRAGILAVPKGQVGAGKAIGLSRFQVFRYVELPQVWAVAVPAIGANILFLMKETSV
                     VSTVGIAELLFVTKDVIGMDYKTNEALFLLFAAYLIILLPVSLLARRIENRVRSAKYG
                     V"
     gene            complement(13613..15097)
                     /gene="tldD"
                     /locus_tag="ACSIGJ_001288"
     CDS             complement(13613..15097)
                     /gene="tldD"
                     /locus_tag="ACSIGJ_001288"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HHQ1330141.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLCPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQIDQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGGA
                     GGGGRYDLAYFDETLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRQSYASVPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFGASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      15172..15276
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            15342..16568
                     /gene="cytX"
                     /locus_tag="ACSIGJ_001289"
     CDS             15342..16568
                     /gene="cytX"
                     /locus_tag="ACSIGJ_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HHQ1330142.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKCGSVLFSVANMLQLAGWTAVM
                     IYVGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARRTGGLKTVSMLLMLLAV
                     LWLSVEVFASSGTNAAPAVSDGMTFGTAVELSAVMPLSWLPLAADYTRQARRPFAATL
                     TATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGITGILAVVLSTVTTTFLDT
                     YSAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLI
                     ADFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVS
                     VRLFFKKTQSLQRNPS"
     gene            16565..17665
                     /locus_tag="ACSIGJ_001290"
     CDS             16565..17665
                     /locus_tag="ACSIGJ_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HHQ1330143.1"
                     /translation="MTRIAVLGGGLSGRLTALQLAEQGYQIELFDKGTRQGEHAAAYV
                     AAAMLAPAAEAVEATPEVIRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADEIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPQDLQAQYDWVIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIAAGLRPTLNHHNPEIRYSRERRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIGRQD"
     gene            17687..18304
                     /gene="thiE"
                     /locus_tag="ACSIGJ_001291"
     CDS             17687..18304
                     /gene="thiE"
                     /locus_tag="ACSIGJ_001291"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HHQ1330144.1"
                     /translation="MTFPPLKSLLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGNE
                     LKREIARCVAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPGYIASGAIFQTTTKQMPTAPQGLDKLREYVEQARGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT         3925 a         5145 c         5216 g         4261 t
ORIGIN      
        1 cagactggga aatagataaa gttaataagt taatcatatt aaaagggtta gactctgaaa
       61 aaataatttc tctaaattat gattaaggat gcttggttat ttaagatatt ctccccatta
      121 tataatacag gttagctaaa aactaggaaa ctgttccttt actgatcggc ttgaacggcg
      181 gcggtaacac ctatgctaac cctaatagat tgccattttt ataggctagt tgaaatagcc
      241 gttctatttt ttcaataact ctaaataaat taaaagttat tttacaaagt agaaatacga
      301 tgtcctctcc agtaacagcc atccctgacg gtcagttcgt ccgtgtagtc gatactggtg
      361 ctgtttttga tttttgccca atccgtaatc tttagattac caatgggaaa ccgcctgtta
      421 caaataaaaa accctgcgat aagcagggtt ttttgaattt ccaaaatcaa cgtttggaga
      481 attgttttgc acggcgtgct ttgcgcagac ccggtttttt acgttcgact tcgcgcgcat
      541 cgcgggtaac gaaaccggct tgagacaagg caggtttcaa agcagcgtca aaatcaatca
      601 gggcacgggt aataccgtgg cggattgcgc cggactggcc ggtttcgccg ccgccgacaa
      661 catttacttt gatgtcgaga gattcggcgt tttcagtcag aaccagaggt tggcggacaa
      721 ccatacggct ggtttcgcgt gcgaagaatt cgtcaacggg acggccgttt acgatgattt
      781 gacccgtacc tttagtcagg aatacacgag ccactgaact tttgcggcgg cctgtgccgt
      841 agtagtattt accgttcatg tcgtgtcctt attatttcag ttccaaaact ttgggttgtt
      901 gcgcagcatg ggcatgctcc gcaccagcgt acactttcag ttttttaatc atggcgtaac
      961 ccagcggacc tttgggcagc atacctttta cagcctgctc caaagcgcgg cccgggaatt
     1021 gatcttgcat ttcgcggaaa gtgcgctcgt agatgccgcc tggaaaaccg gaatggcgga
     1081 agtatttttt atcttcgaat ttggcaccgg ttacacgcag tttgtccgca ttgatgacga
     1141 tgatgtaatc gccggtatcg acgtgggggg tgtattcagg tttgtgtttg ccacgcagac
     1201 ggctggcgac ttcggttgca acgcgaccca agactttgtc ttgggcatcg atgacgaacc
     1261 attcgcgctt cacctcgtgg ggtttcgctg aaaaggtttt catagtggaa atccagatag
     1321 atatagaaag ttatggattt taaagacagg atttgatttt gtcaatcgca ttaccgcgtt
     1381 acggaaggat tttccggatt tcggcagact gcatactgct ttttcgggcg gggcggcggc
     1441 caatgtgaaa aaccgcatcg ttgcgatgcg gttttgaatg ggaatccccg cgagagccgt
     1501 ttcggccgaa tccgcttgaa ccttgctgac aaggcggctg ccccgggtag tttcgggtgc
     1561 gtccgcaaaa ggacgctcgc gcccactact gctcccggca accttaagcg aacttattgg
     1621 ttcaaaggaa tatatgcctt cgcggacacc gcagggaaaa aggggttatt cctgccccaa
     1681 gcgggatagt gccttttggc aggcgttgtc catatcggtt attttacgcg caaaatcgcc
     1741 gattgccaaa tcgccgccgt tcagggaggt tttcaatagg tcgtggacga cgttgagcgc
     1801 ggccataatg acgatttttt cgctgtccgc gacgcggccg ccttcgcgga tggcttcggc
     1861 tttgccgttg agcattccga ctgcctgcaa cagtgtgtct ttttcttctg ccggcgtgtt
     1921 gacggtcagc cgggcgtgca tgacttcgat gtagacttgt tcgatgttca tcctttaatc
     1981 cttattgctg cgtttcctgc cgttggggga ggcgcgctgc cagtgcgctg attttctccc
     2041 tgctctgttc gagcagggcg cggtatcgtg cattttcttc cgtcaggctg tcgattttgt
     2101 tttgcaggtc ttctttgagt ttgccgactt ggacgagcag ggcttcgctg agttcgtcga
     2161 cggcggtctc gtgttcgagt ttttgccgct cgtgcgcccg ttcgagttcg gcgacggttt
     2221 ctttgaggcg gcggttttcg ctgacgaggg tttcgaattt ttgtaccaac gtataaacgc
     2281 tgctttcgag tttttcgata ttttgtttca tgaccttacc tgtctgtatg ccgtctgggg
     2341 gttcagacgg catctgtttg ttgtttattc gaaacgcgcg ctgcgttcca tcagcctttc
     2401 gacgacctgc tgaggggtca tttctttgcg gatgagttgc agcagggttt gggtaatcgg
     2461 catatcgatt tggtatttgc aggcggtatt gaagacttct tctatggtgc tgaccccttc
     2521 ggaaacgtgc ccgatttcga ccagcacctg atgcagttcc ttgccttctg ccaaacccaa
     2581 gccgacgcgg cggttgcgcg aaagtgcacc ggtacaggtc aagatgagat cgccgatgcc
     2641 ggcaagcccc atcatggttt tgggctgcgc gcccattgcg gaggcaaggc gggtgatttc
     2701 ggccagtccg cgcgtaacca gtgcggcacg ggcgttgagt ccgtattcga gtccgtcgga
     2761 gagtccggta gcgattgcca tgacgttttt gactgaaccg cctactgcca cgccgataac
     2821 gtcggtactg ccgtaaagcc tcatgacggt cgtgttgagc tgcggtacga gttcttcaat
     2881 ccactcttgg ttttcggagg cgaggacgac ggcgcagggc agttgtttgg cgagttcctg
     2941 tgcaaaactc ggaccggaaa gtacgccgat tttcttattg tcgggcaata cttctttcaa
     3001 gacttggaag gtcagcagcc ctgtatcctg ctcgaagcct ttgcaggcgg cgaggacggg
     3061 gaggtgtccc gcgccgtact gtttgagcag ctctgcgctg cttctcaatc cggcaacgga
     3121 ggttacgata aggacaagtc cgctgtcttt gagcgcctct gccaaatccg cacacacttc
     3181 caaggtttcg ggaaaggaaa agccgggcag tccgcgtttg ttttcacgcg cttcctgcat
     3241 ttggcggact tggtctgcgt tgcgcgtcca cagggatacg cggttgccgt gttgggaaaa
     3301 atgcagggcg agcgccgtac cccacgaacc tgcgccgata acggtaattt tcattggtcg
     3361 tctttcaaca tatcactgcc gttcacttta aaacaatcgg tgtttctctg caagtgcggt
     3421 caggtaaatg ccgtctgaaa ggcgttcaga cggcattttg ccccgatgcg gcactatcag
     3481 ccggtattgc gcaaaccttg cgccacgccg ttgatggtca ggtgcaccat cagcagggcg
     3541 tgcggattgt cgggttcttt gcgcaggcgt ttgagcatgg cgacttgcaa accgttgagc
     3601 gcgttcaggt atggaatcct caaagcgagc gaacgggcga ggctgcggtt gtcgcgcaaa
     3661 agttcttcgg tttgcagcag gtcgagcagg gctttgcggc tgcgtcggta ttcttcctta
     3721 atcatgccga agatgacttt tgccttttcg ggtgattcgc tcaaaccggc gtagttttcc
     3781 gccaaggtga tgtcggtttt cgccatcact tgttccatat tggagagcat cgcttggaag
     3841 aaggggttgt tttgggcgtg tccgcgcagg gcggcgaggg tttcggggct gccttcgcac
     3901 aaggtttcca ccgcgctgcc gaaaccgtac caagccggca gcatgaggcg gttttgcatc
     3961 caggagaata cccacggaat cgcgcgtaaa tcctgaattc gcgccagggt tttgcggctg
     4021 gcgggacggc tgccgaggtt gagggtggcg atttcctgaa tcgggctggt ttgcaggaag
     4081 tagtcgatga agtcggggtg ggtaatcagt tcgcggtagt atttgaacga tacgtccgac
     4141 aatgcctgca tcagtttggc atcagggtct tttttatccg gcaggatgct ggcttccaaa
     4201 gtcgcggcaa ccaaggtttc caagttgcgt tgggcgttgc cggggtcggc gtatttggcg
     4261 gtgatgactt cgccttgctc ggtaatgcgg atttgtccgg caacgctgcc tgcgggttgc
     4321 gccaagatgg cttggtaaga cgggccgccg ccgcgtccga cgctgccgcc tcgaccgtgg
     4381 aacaagcgca tgcggacatc gtattttttg aagagttcga ccaagcccaa ttccgcctga
     4441 tgcagacacc atgagctggt aacgtagccg ccgtctttgt tggagtcgga atagccgagc
     4501 atgatttctt ggatgttgcc gcggctttcg agcagggcat cataccagtc gaggcggaac
     4561 atggtttcca tgaccggaca ggcattttcg agcgcttcga tggtttcaaa cagaggcacg
     4621 atgttgatgc ggctgtgcgg tttgccgttt tccaccgcca acaggccgct ttctttcagc
     4681 agcaatgcca aggcgagcag gtcgccgggt tgttcgcagt tggaaataat gctttgtgtt
     4741 acggcatctt cgccaaattc gtctttgatt ttgcgcgctt cgttgaaaat cgccaattcg
     4801 cggcgggtat ggtcgctgta tgtgatgaac gggctgtaca gcggacgttg gtggcccaat
     4861 tcgcgcaaca aggcggcttg tttttgctct tcgttcaggc tgttgtagtc ttccaagcct
     4921 gcgtgttgga aaagctcggc aaccacatcg gcgtgtttgc ctgcgtgttg gcgcaggtcg
     4981 agcggcatca tgtggaagcc gaacacggac acggaacgga tgaggtctgc caaacggcct
     5041 tcggcaagca ggcggctgcc gttgtcgata agggaacgtt gcaatttttt caaatcatcg
     5101 agaaattttt gggccgaagc ataaggctcg agaaagccga atttgcagcc catgcccaaa
     5161 ccgagcgagc gcgctttgcc catagcgcgc gccataatgt aggcaatggc gcggcggtaa
     5221 ggttcttcgg tgcgggcgat ttcttcgtca ggcgagaggg ctgccagtgc cattacgtcg
     5281 tcgttgactt tgacgcggcg gatggaaagc ggcagttcgc ggtaaagttt gtcgagttcg
     5341 ctgcggtaaa aacggaacac ggcatcggcg tggcggcgga aggcaaagcg cagggtttcg
     5401 ccagaaacaa acggattgcc gtcgcggtcg ccgccgatcc agccgccgat tttaaggatg
     5461 ttcggaacgc ggacatcggg ataggccgtc tgaaagtcgt gttccatctt gcggtagagt
     5521 ttgggcaggg cttcaaaaaa gctcatcggg aagatggaca cgccgttgtt gatttcgtcg
     5581 ttgacgctga gtttgtggcg gcgcgtttcg ctggtctgcc acaagcccag aagcacggtg
     5641 tcgatttcgc ggcgcagccg tgccagcgcg tcggcattgg tgcagcgttc gcgttgcggc
     5701 agcagcgcgc ggatgcggcg gttgaaattc aaaacggttt ggcgttgcac ttcggtcggg
     5761 tgcgcggtca aaacggcggt aacggacgta ttgtccaact gccgctgcac cgatttgccg
     5821 tcggctttcc ccgctttgag cctgcggacg gtttccgtca ggctgccttc tgctgcgttg
     5881 tggccggcat cttcgtggat ttggcggcgg cgttcgtggt gcacgtcttc ggcgatattc
     5941 agaatctggg cgaacagccc gcaggcaagc gtcagatcgt aggtctgccg ttcgtccaat
     6001 tgcggcaata ctttttcaat caatgccgcg ctgtcgtcgg aagtggacaa gagtttgacc
     6061 gtttcgacaa ccaacggcga ggcttcttcg tgcaggaggt tgaacaggga ctgtttcaaa
     6121 aattccgcgt ccgccgccaa agccgcgtcc ttcggattgt tcaggatatg cagttgcatg
     6181 attttcctct cattgccgta aatactgtaa atgtacctca aatgccgcat ccgtgccaaa
     6241 ccgttcacac tttaaccact catgtcccga aatgccgtct gaagttgaac gccgcccgac
     6301 ggcggcgtta caatcgcccg caactgtttt ttccgaacat catcatgacc gcgaccgaac
     6361 acgacaacga cgacgcactc ctgctgcggt acagccgcca catcctcttg gacgaaatcg
     6421 gcatcgaagg gcagcagaaa ctttccgccg cgcatatttt ggtcgtcggc tgcggcggat
     6481 tgggcgccgc cgccctgccc tatctcgccg cctcgggcgt cggcacgctg accatagccg
     6541 attccgacac ggtcgagctg cacaacctgc aacgccaagt cgcatttgac gagggcgatg
     6601 tcggcaagcc taaagccgaa accttggcag gccgcctgaa acgcatcaac catactgtcg
     6661 atgtccgcgc cgtcaacgaa aaactcgacg gctgccgcct gaccggtttg gttcaaaccg
     6721 ccgacatcgt tttagactgc tgcgacaact acgccacgcg gcaggcggtc aaccgtgcct
     6781 gcgtgcaaac gaaaacaccg ctggtttcag gggcggcggt acgttttgaa ggacagcttg
     6841 ccgcataccg tcccgacttg cccgactcgc cgtgttacgc ctgcctgttc gacggcggat
     6901 cggcttcaga cggcatctgt tccctcttcg gcgtgctctc gccgctggtc ggcatcgtcg
     6961 gctgcacaca ggcggcggag gctctgaaaa tcctgctgga agcgggcgaa ccgtcgcacg
     7021 gcaggctggc ggtttaccgt gccttggaag ggggctggca atatttcgac ctgccgcgca
     7081 accccgaatg cccggtttgc ggcgcggcac ggtaaaaccc tgccgccgtt tcagacggca
     7141 tccgaacgga tgcggcggaa cggttttaaa aatttaaaaa tttacatttc tttgcaaaaa
     7201 aataaactta ccttataatt gcagttgttt tagcaatgtc tgtttcgcag actcattgag
     7261 taagacgttt tccccgtaat gtgtttggcc gtctgtcccc tttgggttcg gacggctttt
     7321 tttggctgtg tttgaatacc cggttggttt tatctgtttg cagcggggga agccgctcat
     7381 cgccgttcgg acaagaaccg gtttcatcgg ataaaaggca ttttgtccga ctgattaagg
     7441 ttacatctta gggttgcacc ggcggaatat tcaaacacag cctttattta aggaaatccg
     7501 gatacggcgg cgcatcaata atgcggcgga atctcgtcgc gcagggaata cggctcttgc
     7561 gcgtcgggat tcctgtcctg cattttttga tacagcagcc tcaactgagc ctgctgcaaa
     7621 tccaacgtct gccgcaattc cgccaccatc gcgttcagac cggagattac gtcctcctga
     7681 agcgcggttt ggatttccag ttcgacaata cggcgttcca actcttgaac cgcgtccatt
     7741 tacagcacca tcgcggcaat ccagccggca atcagaagcg ggatgttgta gtggatgaag
     7801 gtcgggatga cggaatcgcg gatatggtcg tgccgcccgt cggcgttcag ccccatcgtc
     7861 gggcccagcg tggaatcgga cgcgggcgaa ccggcatcgc ccaacgcccc cgccgtgccg
     7921 acaatggcgg cggtggcaag cggcgaaaag cccaaaccgg tacacagcgg cacataaatc
     7981 gcggcaataa tcggcaaagt ggaaaaggac gaaccgatgc ccatcgttac caaaagcccc
     8041 accaccagca tcgccaacgc cgccatacct ttgctgttgc cgaatatcgc catactgctt
     8101 tccaccagcg gctgaatatg cccggtcgca ttcatcacgg cggcaaaacc ctgcgcggca
     8161 atcataatga agccgaccat cgccatcatc ttgatgcctt cgccgaatac gtcgtttgcc
     8221 ttgtcgcggt taatgacccc caacatcata aatacggcga aaccgagcat cgcgcccaac
     8281 accagcgaat cttcatacat caactgtatg gcaaagcata cggcgatggc ggcggcggca
     8341 accaggctgc ggtaggcgga cggctgcgga cggtttgccg catcggcgtt gcccgccgta
     8401 tcggcattgt tgctttggta cagtcgcggt ttgcggtagt ggacaaacgc cagcaggagt
     8461 ccggccagca ttcccaatgc gggaatcgcc attgccgcca tcacgttgat gtttttcaca
     8521 tcaagctgcg gcgcggcgga atggatgttg cccaacagga tttcgttcaa aaaaatcgcg
     8581 ccgaagccgt aaggcaggaa catataagtc gtaaccagcc cgaaagtgat gacgcacgca
     8641 atcaggcggc ggtcgatttt caggcggtta aacaccaaaa gcagcggcgg aacaatcatc
     8701 gggataaagg cgatgtggat gggtatgacg ttctgactca taatgcccat cacaaggatg
     8761 atggaaagca gcagccattt gaccgcgccc tcgcccgaac gcacgctgtc gggcataccg
     8821 ccccggttca gcttgcggac gaccgcgccg gcaagctgct gcggcaggcc ggaatgggta
     8881 atcgccatcg caaacgcacc aagcatcgca taagaaagcg caatcttcgc gccgccttcc
     8941 aaacctttgt tgaacacggg gataatcccc gcctgactga cctgtcccgc cgcatcggca
     9001 atgttttgca gcggcatacc cgccaccgcg ccgccgacaa acgcgccgac cgtcaggctc
     9061 aataccacgt gcacgcgcga cagcgacagc accagcataa cgattacggc aactacgact
     9121 gcattcattg tttatcgctc caaacctata aatgtttaca tatcaaaaca catcataacc
     9181 caataacgga aaatccgcca attttgcaaa caattatttc aaatgcttca tatacttccc
     9241 cagcgtaacc ctgtccaaac cggccaaatc cggcaggatt tccactcccg aaaaaccatt
     9301 ctccgccaac acgccgcgca ccgccgcgcc ctgatcgaaa ccgtgttcca acaacaaaaa
     9361 accgccttcc gccaaacggt cgggcgcgcc ttgcgccaag gtgcggatgc agcttaggcc
     9421 gtctgaaaag tcggtcagcg cgatttgcgg ctcaaaccgc aaatcgcctt gcgacaaatg
     9481 tttatcgccg ttttcgatat agggcgggtt ggacacgatg atgtcccatt gcctttcaga
     9541 cggcatatcg gtgtcgaacc acgaaccgtg tgcaaattcg acccgcgcgc ccaaatccgc
     9601 cgcatttttc cgcgccgttt caagggcggg cgtgctgatg tcggatgcgc gcacaaacgc
     9661 atcggggcgt tcgagcgcga cggttacggc aactgcgccg ctgcccgtcc ccaaatccca
     9721 cacgcgcccg ttttcgggca ggcgcgccaa tacggcttcg accaagtgtt cggtttcggg
     9781 gcgcggaatc agcacgttcg ggttgactgt aaagcgtctg ccataaaatt cgcgcacacc
     9841 taaaatatag gcaaccggct cgccgttcag acggcgttgc gccagcctgt ccgcccgctg
     9901 tcggatttcg tccggcattt cttccccgcc ccgcgtcaac aactgcacgc gcgtatattc
     9961 cgaaacatat tgcagcagca tccttgcttc aattttaggc agttttgaca agcccaacca
    10021 ttcgtcaaac gtcatttttc gtcccgtctg ccgccgatgc ggcttgaaca ttttttccgg
    10081 ctttgcggca cgcttgttca aaaaatatca ttttgcattg aaattatata ataaaggaaa
    10141 acaccttatc gccgtttcaa taccgtagga atttatccga ataacagaaa cgctccgccg
    10201 tcatcccgcg aaaacggtca tccggaaaca taaggctgaa gcgttttatc tggaatgacg
    10261 atttggaagt ttcccgaaat tcaaaaacag gcaaaaccgg acgaacttga tttccgcacg
    10321 cgcaagaata ataaaataaa taaagcgatg agatagttga tttttccttt gaatttgcaa
    10381 tattttcggc ggctgaaaaa aaatccgcac ccgccttcaa atgcggatac ggattttctt
    10441 gtcaaacaaa cttattcggc caacaatgct tccggtttga ctttctcgcc atacacgggc
    10501 aagagggttt tttcataggc ggctttcaga cggccgtctt ttttcatggc ggcgatttcg
    10561 ccgttgaccc agttcaggag gtcggcgttg cctttttgaa cggcgggggc gataaattcg
    10621 gcaggaccga gattgccgat ggcgacttca aagttcgggt tttctttcgc ccacgcccac
    10681 agcagggcgt tgtcgtgcgc gagtgctacg ccgcgaccgt ctttcagcgc gtcgaaggtt
    10741 tcagtgtttt ggtcgaattt caacagcttg acttcgggat ggcttttggt gaagaaagcg
    10801 tcggcggtgg tgcctttgtt gaccagcagg gtttggtctt tcaattgcgc catgtcggta
    10861 atcggtttgt ttttggggga aaccacgccc aacgccactt tcatgtaagg atcggcgaaa
    10921 tcgacggctt cggcgcgttc gggggtttgg gtaaagttgg cgaggatgag gtcgactttg
    10981 ccggaacgga cgtattcgac gcggtttgcg gcctcggtca ggacgaattc gactttgtcg
    11041 gggctgccga gcaggtcttt ggccaggtct ttggcgattt caacgtcaaa gccttggttt
    11101 ttgccgtttg cgtcaacata gccgaacggg ggtttgtcgc cgaatacgcc gatgcggata
    11161 acgccttttt ctttgatggc ggcaacggtt gccgcaccgc tgctttgtga agattgggca
    11221 tcgccggagc cgcccccgca ggcggtcaga ccgacggcga tggcggcgga agccaaaagg
    11281 gctttgagtt tggcgttcaa tttcattgga aatctccttt ttgaatgttt gcttgggatg
    11341 tttcaacaca caggacgaca cataaagcac tgccctatga aatgccgtct gaaaagggtt
    11401 cggacggttt gcggtaaatc agtagtccat acctgccaga aattggcggg cgcgttcgct
    11461 ttttggtgcg gaaaaaaagg tttcggggtc ggacgattcg acgatgccgc ctttgtccat
    11521 aaagacgatg cggtcggcaa ctttgcgtgc gaaccccatt tcgtgggtta cgatgagcat
    11581 actcatccct tcgcgggcga gttccaaaac cacttccaag acttcgcgca ccatttcggg
    11641 gtcaagtgcg gcggtgattt cgtccagcag gatgacttcc ggattcaggc acagggcgcg
    11701 gacaatggcg atgcgctgtt tctgaccgcc ggaaagttcg cgcggatagg cgtttttgcg
    11761 gtctagcagt ccgacgcgtt ccaacagttt gccggcttgc gcctctgctt cggcacggtc
    11821 gcggttttgt acctttaccg ggcctaagag gatgttttcg atgacggtca tgtgggcaaa
    11881 cagttcgtag ctttgaaaga ccataccgac tttttgccgg gcggtttgcc aggaaacgtc
    11941 tttgccgaat tcgccgacac cgtccatcac gatgctgccg ccttggtgcg gctccaaacc
    12001 gttgacgcag cgcaggaggg tggatttgcc gcagccggac gggcccagca gtacgatgac
    12061 ttcgcctttt tccaagtcca agtctaagga ttggatggcg gttacgctgc cgtattgttt
    12121 gtgcagcttg cggatgctca gtaaagccat gtcagtgttc ccatttttgt tcaagttttg
    12181 ccgccagtag agatagcggc cagcaacaga agaaatacag cataaaaatc aggccgtaaa
    12241 cccaaaatga agcattgggc tgcgtcagca acgagttttc aataatttgc tgcccgactt
    12301 tgacgacttc gatcacgccg ataagccagg cgagcgagct ggttttgatc atgcgcgtga
    12361 acaggttgac cgcgccgggc aatacgcggc ggatgctttg cggcagctcg atgcagcgga
    12421 atacctgccc ccggctcaag ccgggcgcaa gacccgattc gacctggtgt ttttcaatcg
    12481 attccagtgc gccgcgcacc aagtcgccca tttcggcaac gccccacagg gaaaataccc
    12541 agacgcagac ccaaaatccg ccgatatgga tgcctgtcca gacggatagg ccgaaataca
    12601 ggccgaacag ccacaccaaa atcggcacga ttcggatggt ttcgagataa aaccgtccga
    12661 caaagcggac aagccggttg cgcgaacgca aaaccaagcc gaacagcgtg cccaatacgc
    12721 aagaagccgc aacagagatt aaagaaattt gtgccgttaa gagcaggcct tcgcccaacc
    12781 gtgccgcatt ttttccttca aacagccatt caaacgccat atttcgcact ccgtacgcgg
    12841 ttttcaatcc gccgcgccaa caaagaaaca ggcagcagga taatcaaata agcggcaaac
    12901 agcaggaaca aggcttcatt ggttttgtag tccataccga tgacgtcttt ggtaacaaat
    12961 aacaattccg caatgccgac cgtgctgacg acggatgttt ctttcattaa aaacaatatg
    13021 tttgcgccga tggcgggaac ggcgaccgcc caaacctgcg gcaattcgac atagcggaac
    13081 acttgaaagc ggctcaaacc gattgccttg cccgctccga cctgcccttt ggggacggcg
    13141 aggatgccgg cgcggacagc ttccgccata tagcttgcgc ccaagaaaac cagcgcgatt
    13201 acgccgcagg tgaaaccgtc ccatttgatg cccattttcg gcagaccgta atataggaaa
    13261 aacaattgga tcagcagggg cgtattgcgc gacagctcga tataggcgcg cgccaatgcg
    13321 tagaaggggc ggatgcggta tgccgttacc acggcgacgg gcaggtcgaa cagcaacgac
    13381 aaaacaacgc cgtaaacgga caattccagc gtcagctttg ccgcatcggc gaatttgggt
    13441 acggcatcga ttaaataagg ccagttcatc agaaatcagc ttaacgaaag gcggtatttt
    13501 aatgaacttg taagataatg ttaaagaatg cttaggaata tcaaacatcg gaaaaaagaa
    13561 taaggcgatg ccgtctgaaa acctttcaga cggcatcgtg ccggattccg cgtcagattg
    13621 cgctgccgcc gacggtcagc ccggcatcga tacgcagggt cggctgcccc acgccgacgg
    13681 ggacgctctg cccttctttg ccgcacacgc cgacaccgct gtcgagcgcg gtatcgttgc
    13741 ctatcatcga aacgtgtttc agcacttcgg ggccgttgcc gataatggtc gcgcctttga
    13801 cggggtattg cagcctgccg ccttccaccc accacgcttc ggacgcgccg aagacaaatt
    13861 tcccgctggt gatgtccacc tgtccgccgc cgaagttgac ggcgtaaatg cccttgtcga
    13921 tggacgcgat gatttcttcc ggctcgtagc tgccgttttc cataaaggtg ttggtcatac
    13981 gcggcatcgg gacggaggcg tagctttggc ggcggccgtt gccggtcgat tgcgtacccg
    14041 tcaggcgggc gttggtttcg tcctgcatat agccgaccaa gatgccgtct tcaatcaata
    14101 cggtgcggcg tgtttcgttg ccttcgtcgt cgatgttgag cgaaccgcgc cggccggcaa
    14161 tatcgccctg atcgacgacg gtaacgcctt tggcggcgac gcgttcgccg atccggccgg
    14221 aaaagacgct ggtccccttg cggttgaaat cgccttccaa accgtgtccg accgcttcgt
    14281 gcagcaacac gcccggccaa ccgttgccca aaacgacggt catctcgccg gcaggcgcag
    14341 gacgggattc gagattggtc agggcttgtt tgacggcggc atcgacaaac tgccgcacca
    14401 aggtttcatc gaaataagcc aagtcgtagc gtcctccgcc gcccgcgccg ccctgttcgc
    14461 ggcgttcgcc ctgtttggcg atgacggtaa cgttcaggcg caccatcggg cggatgtcgg
    14521 cggcgtgttt gccgtccaga cgggcgaggt aaaccatatc gtattcgcag gtcaaaccgg
    14581 ccatcacttg cacgatgcgc ggatcggcgg ctttggcgat tgcttccact ttgttcaaca
    14641 gcgcgacttt ggcggcggaa tcgaggccgg caatggggtc ggatgcggaa caaaccggct
    14701 tgccgcgcgt ttcagacggc attttggcgg acaccttgcc gcctgccgcc ccaatcgcgc
    14761 ggacggcgcg ggcggaacgg tttatcgaat cgatgcacag gctgtcggcg taggcaaagg
    14821 cggttttgtc gcccgaaacg gcgcgcacgc ccacaccctg atcgatttgg aagctgcccg
    14881 atttgacgat gccctcttcc aaatgccagc tttcataagc ggtgcgctgg cagtagatat
    14941 cggcataatc gacgtggtgc gcgccgatga tgcacaggct tttggcgagc agttcggggc
    15001 aaaggcggtt ggcttcgagc agccgcgcct gtacggcgga ataggtcgga tgcatagtgt
    15061 cggcgcataa aaaatcaggg gcttgattat acggcatttg ttatggaaat gcgccgtgcc
    15121 gtcgaaaatg taagaatttt gccaacgcac gttgctttgt gtaaacttaa agccttcttg
    15181 tcggagtgcc gcccgccggg cggctgagat tgcgaaagca aaatccgtag aacctgtcgg
    15241 ggtaatgcct gcgtaggaaa caaaacctcc ccgccctttc gggcttccgt tcccttttcc
    15301 gcacttcccc gcccaccttc cttcttttta aaggacgtca tatgtcgggc aatgcctcct
    15361 ctccttcatc ttccgccgcc atcgggctga tttggttcgg cgcggcggta tcgattgccg
    15421 aaatcagcac gggtacgctg ctcgccccct tgggctggca gcgcggtctg gcggccctgc
    15481 ttttgggtca tgccgtcggc ggcgcgctgt tttttgcggc agcgtatatc ggcgcactga
    15541 ccggacgcag ctcgatggaa agtgtgcgcc tgtcgttcgg caaatgcggt tcagtgctgt
    15601 tttccgtggc gaatatgctg caactggccg gctggacggc ggtgatgatt tacgtcggcg
    15661 caacggtcag ctccgctttg ggcaaagtgt tgtgggacgg cgaatccttt gtctggtggg
    15721 cattggcaaa cggcgcactg atcgtgctgt ggctggtttt cggcgcacgc agaacgggcg
    15781 ggctgaaaac cgtttcgatg ctgctgatgc tgcttgccgt gttgtggttg agcgtcgaag
    15841 tgttcgcttc gtccggcaca aacgccgcgc ccgccgtttc agacggcatg accttcggaa
    15901 cggcagtcga actgtccgcc gtcatgccgc tttcctggct gccgctggcc gccgactaca
    15961 cgcgccaagc acgccgcccg tttgcggcaa ccctgacggc aacgctcgcc tatacgctga
    16021 cgggctgctg gatgtatgcc ttgggtttgg cggcggctct gtttaccgga gaaaccgacg
    16081 tggcgaaaat cctgttgggc gcgggcttgg gcataacggg cattctggca gtcgtcctct
    16141 ccaccgttac cacaacgttt ctcgatacct attccgccgg cgcgagtgcg aacaacattt
    16201 ccgcgcgttt tgcggaaaca cccgtcgctg tcggcgttac cctgatcggc acggtgcttg
    16261 ccgtcatgct gcccgttacc gaatatgaaa acttcctgct gcttatcggc tcggtatttg
    16321 cgccgatggc ggcggttttg attgccgact ttttcgtctt aaaacggcgt gaggagattg
    16381 aaggctttga ctttgccgga ctggttctgt ggctggcagg cttcatcctc taccgcttcc
    16441 tgctctcgtc cggttgggaa agcagcatcg gtctgaccgc ccccgtaatg tctgccgttg
    16501 ccattgccac cgtatcggta cgccttttct ttaaaaaaac ccaatcttta caaaggaacc
    16561 cgtcatgacc cgtatcgccg tcctcggagg cggcctttcc ggaaggctga ccgcattgca
    16621 gcttgcagaa caaggttatc agattgaact tttcgacaag ggcacccgcc aaggcgaaca
    16681 cgccgccgcc tatgttgccg ccgcgatgct cgcgcctgcg gcggaagcgg tcgaggcaac
    16741 gcccgaagtc atcaggctgg gcaggcagag cattccgctt tggcgcggca tccgatgccg
    16801 tctgaacacg cacacgatga tgcaggaaaa cggcagcctg attgtgtggc acgggcagga
    16861 caagccatta tccagcgagt tcgtccgcca tctcaaacgc ggcggcgtag cggatgacga
    16921 aatcgtccgt tggcgcgccg atgaaatcgc cgaacgcgaa ccgcaactcg gcggacgttt
    16981 ttcagacggc atctacctgc cgaccgaagg ccagctcgac gggcggcaaa tattgtctgc
    17041 acttgccgac gctttggacg aactgaacgt cccttgccat tgggaacacg aatgcgcccc
    17101 ccaagacctg caagcccaat acgactgggt aatcgactgc cggggctacg gcgcgaaaac
    17161 cgcgtggaac caatcccccg agcacaccag caccttgcgc ggcatacgcg gcgaagtggc
    17221 gcgggtttac acgcccgaaa tcacgctcaa ccgccccgtg cgcctgctgc acccgcgcta
    17281 tccgctctac atcgccccga aagaaaacca cgtcttcgtc atcggcgcga cccaaatcga
    17341 aagcgaaagc caagcccccg ccagcgtacg ttccgggctg gaactcttat ccgcgctcta
    17401 tgccgtccac cccgccttcg gcgaagccga catcctcgaa atcgccgccg gcctgcgccc
    17461 cacgctcaac caccacaacc ccgaaatccg ctacagccgc gaacgccgcc tcatcgaaat
    17521 caacggcctt ttccggcacg gctttatgat ttcccccgcc gtaaccgccg ccgccgtcag
    17581 attggcagtg gcactgtttg acggaaaaga cgcgcccgaa cgtgatgaag aaagcggttt
    17641 ggcgtatatc ggaagacaag attaaagccg cccgaaagga caccctatga ccttcccgcc
    17701 cctaaaatcc ctacttaaat tctacgccgt cgttcccacc gccgattggg tggggcgcat
    17761 ggtcaaagca ggtgccgaca cggtgcaact gcgctgtaaa accttgcacg gcaacgaatt
    17821 gaaacgcgaa atcgcccgct gcgtcgccgc ctgtcaaggc agccgcacac agcttttcat
    17881 caacgaccac tggcgcgagg caatcgaagc gggcgcatac ggcgtacatc tcgggcagga
    17941 agacatggac accgccgacc ttgccgccat cgccgccgcc ggtttgcgtt tgggtttgag
    18001 tacgcactcc gtcgccgaac tcgaccgcgc cctgtccgta caccccggct acatcgccag
    18061 cggcgcgatt ttccagacca cgaccaaaca aatgcccacc gccccgcaag gcttggacaa
    18121 attgcgcgaa tatgtggaac aggctcgcgg cacgcccgtc gtcgccatcg gcggcatcga
    18181 tttgaacaac gcccgagccg tgctggccac cggagtttcc tcactcgccg ccgtccgcgc
    18241 cgtaaccgaa gcggcaaatc ccgaagcggt ggttaaagcg tttcaggctt tgtgggatgg
    18301 ataaaaccga aagaagaaaa ttcaattgcc gtgtaggcaa aacttagccc gttatcgcaa
    18361 atatgcttaa ctttaaatgt ggcatatcat caaattccgt cattcccgct caggcgggaa
    18421 tccgccgtaa aacttgagaa accatcattt gaaaaacagt ttccgaattt caaaaatgga
    18481 ttcccgcctg cgcgggaatg acggcaaccg gccggttgcg tatcaaaaaa taaagtaatt
    18541 cggctag
//
LOCUS       DBJGGI010000037        18200 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.037, whole genome shotgun sequence.
ACCESSION   DBJGGI010000037 DBJGGI010000000
VERSION     DBJGGI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18200
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.037"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            275..973
                     /locus_tag="ACSIGJ_001292"
     CDS             275..973
                     /locus_tag="ACSIGJ_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HHQ1330145.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCARLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLPLARLAAAVDMAAAESKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARAGKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(1030..2034)
                     /gene="amgK"
                     /locus_tag="ACSIGJ_001293"
     CDS             complement(1030..2034)
                     /gene="amgK"
                     /locus_tag="ACSIGJ_001293"
                     /EC_number="2.7.1.221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate/N-acetylglucosamine kinase
                     AmgK"
                     /protein_id="HHQ1330146.1"
                     /translation="MQRQTELKNWLQTVYPERDFDLSFAAADADFRRYFRAAFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGEAAHKALLLEAIGELVGLQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTADTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPAEFDEFYRRFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            2104..4509
                     /locus_tag="ACSIGJ_001294"
     CDS             2104..4509
                     /locus_tag="ACSIGJ_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HHQ1330147.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAADTVAAEEADGRVAEGGAQGA
                     SESAQAFDLTLGSTCLFCSNESGSPERTEAAVQGSGEASVPEDYTRIVADRMQGQSQV
                     KVRAEGSVIIERDGAVLNTDWADYDQSGDTVTVGDRFALQQDGTLIRGETLTYNLDQQ
                     TGEAHNVRMETEQGGRRLQSVSRTAEMLGEGRYKLTETQFNTCSAGDAGWYVKAASVE
                     ADRGKGIGVAKHAAFVFGGVPLFYTPWADFPLDGNRKSGLLVPSVSAGSDGVSLSVPY
                     YFNLAPNFDATFAPGIIGERGATFDGQIRYLRPDYSGQTDLTWLPHDKKSGRNNRYQA
                     KWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGGEEIAGNVNLNRRVWLDYGGRAAGGS
                     LNAGLSVQKYQTLANQSGYKDEPYAIMPRLSADWHKNAGRAQIGVSAQFTRFSQDGRQ
                     DGSRLVVYPGIKWDFSNSWGYVRPKLGLHATYYSLDSFGGKASRSVGRVLPVVNIDGG
                     TTFERNTRLFGGGVVQTIEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYYG
                     NDRINAANSLSTAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKNPRSRSD
                     WVAFASGGIGGRFTLDSSIHYNQNDKRAEHYAVGAGYRPAPGKVLNARYKYGRNEKIY
                     LQADGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEMLAGAEYKSSCGCW
                     GAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPAGRMDVAVPGYIPAHSLSAGRN
                     KRP"
     gene            4611..5612
                     /locus_tag="ACSIGJ_001295"
     CDS             4611..5612
                     /locus_tag="ACSIGJ_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HHQ1330148.1"
                     /translation="MMKIKALMIAAALLAAADVHAAPQKAKTAPAKAVKAAATAQKEA
                     APAQQQGGIRFSDGIAAVADNEVITNRRLAEAVAEAKATLPEDAQISESELSRQVLMQ
                     LVNQSLIVQAGKRRNIQASEAEIDAVVAQNPALKNLSPTQRRELADNIIAEKVRQQAV
                     MQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKADSKNAAVGAESTIRK
                     IYDQARNGTDFAGLARRYSQDASAGNGGDLGWFADGVMVPAFEEAVHALKPGQVGAPV
                     RTQFGWHIIKLNEVRDAGTPEERIRNSVRQYIFQQKAGQATVNLLRDLHSGAYVDIR"
     gene            complement(6092..7972)
                     /locus_tag="ACSIGJ_001296"
     CDS             complement(6092..7972)
                     /locus_tag="ACSIGJ_001296"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HHQ1330149.1"
                     /translation="MNIFYEESGQFKVAVVVQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPVYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLRTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQEGFEIKYFP
                     KGTGFPDLSLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGTKRVGIHIAAPS
                     LAVRQGGGMEQIIMQRLSTVYFPGGKITMLPENWITAFSLDAGAYRPAVSIYFDVDGE
                     FNVGEPTCKIEAVNIAANLRIQAIEPHFNAETGLDQAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDEEGNVSVVRRERGSPIDTLVSEMMILANSTWAQMLD
                     ENGLPGIFRVQPAGKVRMSTQSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQKSDAALFAALRDFDTAYAAYADFQRQMEAYWSLVYLQQQGISELTATILKE
                     DLVRIEGLPLTTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAAAPGGKTAGN
                     AV"
     gene            8128..8760
                     /locus_tag="ACSIGJ_001297"
     CDS             8128..8760
                     /locus_tag="ACSIGJ_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HHQ1330150.1"
                     /translation="MENVNRVPEQARYDAERRQADEALADVFPAVSIFGSARTPQDHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIALPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPVVLVG
                     KAFWSGLAEWINAQLLARGMISEGAASLFSISDDEDEIIAYLSEHGLQTA"
     gene            8832..10202
                     /gene="purB"
                     /locus_tag="ACSIGJ_001298"
     CDS             8832..10202
                     /gene="purB"
                     /locus_tag="ACSIGJ_001298"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HHQ1330151.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAAPEIAEVPPFSAETLAEIDKVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEARETVILPKLAEIIGKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKILQAQEFLGKINGAVGNYNAHMA
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRVNTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDVTWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIGSLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            10282..12148
                     /locus_tag="ACSIGJ_001299"
                     /pseudo
     CDS             10282..12148
                     /locus_tag="ACSIGJ_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            complement(12287..12901)
                     /locus_tag="ACSIGJ_001300"
     CDS             complement(12287..12901)
                     /locus_tag="ACSIGJ_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HHQ1330152.1"
                     /translation="MNFTRLLNQVLSTVQKKGSTFSGSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGKTEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIAAAVGNDEALAA
                     ETYLAARLVCADLSRKETVFLARLSQALELDDNLVESLERQLGI"
     gene            complement(12937..15087)
                     /gene="dinG"
                     /locus_tag="ACSIGJ_001301"
     CDS             complement(12937..15087)
                     /gene="dinG"
                     /locus_tag="ACSIGJ_001301"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HHQ1330153.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LTREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDAWPEKIDDAIWLKVTNDRHGCLKAACPNRP
                     ECPFYLARDMLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGLSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALIDRLTGEFGLFIARIEQISAVWDLLSTVSIEGEEPLAKWIARRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGSFNLILRQTGLLWLPETTT
                     LALKSPFDFEKQGELYIPSIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALHLPGDYLPLLLVQGELPKAVLLQKHHQAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            complement(15270..16568)
                     /locus_tag="ACSIGJ_001302"
     CDS             complement(15270..16568)
                     /locus_tag="ACSIGJ_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HHQ1330154.1"
                     /translation="MPSETALLLYADTRAAHTLVWFRQNLRIRDNAALCAAVAEGSPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLARRGIPLYTAASPAGLVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRSVFGKTDLTPDNGTAHTDFNRYRE
                     VWLDRFSKQPPAGSDLFAAYRQPFPENLPAPPPAALSDGIFLPQNGGETAAWRQWRRF
                     LEQAASYSVLKDFPSRKNTSLMGAYLSVGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHADDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPARPVAGV"
     gene            complement(16558..17469)
                     /locus_tag="ACSIGJ_001303"
     CDS             complement(16558..17469)
                     /locus_tag="ACSIGJ_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HHQ1330155.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHHNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVALELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGPLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            17577..17975
                     /locus_tag="ACSIGJ_001304"
     CDS             17577..17975
                     /locus_tag="ACSIGJ_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HHQ1330156.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            18024..>18200
                     /locus_tag="ACSIGJ_001305"
     CDS             18024..>18200
                     /locus_tag="ACSIGJ_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HHQ1330157.1"
                     /translation="MSRQSLQQAAESRRSVYSLNKNLPVGKDEIVQIVEHAVLHTPFS
                     FNSQSARVVVLFGEE"
BASE COUNT         3896 a         4990 c         5240 g         4074 t
ORIGIN      
        1 aaggggtgta acccttccaa atcagggcaa cgcatagggc ggtgctttat gtgccgtcct
       61 gtgtgttgaa acatcattgt tccccaacag ccgtatccgt ccggggcggc gcgtattgta
      121 cgacagaaac cgcttcggat ggcatcgggt ttgatgccgt ctgaagcggt ttcccgaaac
      181 gaaaacgtcc tttttcggcg gcaaactgta tcaacacgcg aaaatacaat aaaataccaa
      241 tatccgattt atttccaaca tcacggagag ccgtatgaaa tccagacacc tcgccctcgg
      301 cgttgccgcc ctgttcgccc ttgccgcgtg cgacagcaaa gtccaaacca gcgtccccgc
      361 cgacagcgcg cctgccgctt cggcagccgc cgccccggca ggactggtcg aagggcaaaa
      421 ctacaccgtc cttgccaacc cgattcccca acagcaggca ggcaaggttg aagtgcttga
      481 gtttttcggc tatttttgtc cgcactgcgc ccgcctcgaa cctgttttga gcaaacacgc
      541 caagtctttt aaagacgata tgtacctgcg taccgaacac gtcgtctggc agaaagaaat
      601 gctgccgctg gcacgcctcg ccgccgccgt cgatatggct gccgccgaaa gcaaagatgt
      661 ggcgaacagc catattttcg atgcgatggt caaccaaaaa atcaagctgc aagagccgga
      721 agtcctcaaa aaatggctgg gcgaacaaac cgcctttgac ggcaaaaaag tccttgccgc
      781 ctacgaatcc cccgaaagtc aggcgcgcgc cggcaaaatg caggagctga ccgaaacctt
      841 ccaaatcgac ggtacgccca cggttatcgt cggcggcaaa tataaagttg aatttgccga
      901 ctgggagtcc ggtatgaaca ccatcgacct tttggcggac aaagtacgtg aagaacaaaa
      961 agccgcgcag taggcccgtt tgaaaaatgc cgtctgaaac ttggttttca gacggcattt
     1021 tgattgggtt tagaacgtaa agcccgtttc cagttcttca tcgccgacca gttcgaccaa
     1081 gagcgcgtag agcggggcga gttcggcata acggcgcgat acgcggcgca gatagtttaa
     1141 gaaacgcggg atttccggac ggtatttgtc tttgccgtcg cggtagtaca ggcgtgcgaa
     1201 gatgcccgcg accttcaagt gccgctgcac gcccatccat tcgaaccggc ggtaaaactc
     1261 gtcaaactcg gcagggacag gcaagccggc agcccgcgcc ttttcccagt agcggataac
     1321 caaatccaaa acaaactctt cttcccactc gataaaggca tcgcgcaaca gcgacaccaa
     1381 atcgtaggaa atcgggccgt aaagcgcgtc ttggaagtct aaaacgcccg gcctgccgcg
     1441 cgtcagcatc aggttgcgga cgataaagtc gcggtgcacg tagactttgg gctgcgccaa
     1501 caagggcggc agcagcgtat cggcggtttg ctgccaaagt tggcgttgtt tgaatgttaa
     1561 ttcgcgcccc aattcttttg cgacaaacca ttccgggaac aggttgattt cgcgcagcat
     1621 cgtttcacgg tcatattcgg gcaaaacccc ttcacggctt gccttctgca atccgaccag
     1681 ctcgccgatg gcctccagca acagggcttt gtgtgccgct tcgccctgct cctgaagcat
     1741 cgcggtcaaa aacgtcgtat tgcccaagtc gttcaatacc acaaacccca aatccgtgtc
     1801 cgcgtgcaat acctgcggca cattgaccat gtcaaacagc ttctgcacct tcaaataagg
     1861 cgcgacactc atcttgtcgg gcggcgcatc catacagacg acgctgccgc cgtctgaaaa
     1921 cgccgcacgg aaatagcggc ggaaatcagc atccgccgcc gcgaaggaca gatcgaagtc
     1981 ccgttcggga taaacggtct gaagccaatt tttcagttcg gtttgccgtt gcataacagt
     2041 atccgggcat ttcgggttac aataaacact attctaaccg gtaaaccgac ttgaggggcg
     2101 attttggctc gtttattttc actcaaacca ctggtgctgg cattgggctt ctgtttcggc
     2161 acgcattgtg ccgccgatac cgttgcggcg gaagaggcgg acgggcgtgt cgcagaaggc
     2221 ggtgcgcagg gcgcgtccga atccgcacaa gctttcgatt tgaccctcgg ttcgacctgc
     2281 ctgttttgca gtaacgaaag cggcagcccc gagagaaccg aagccgccgt ccaaggcagc
     2341 ggcgaagcat ccgtccccga agactatacg cgcattgttg ccgacaggat gcaaggacag
     2401 tcgcaggtta aggtgcgcgc ggaaggaagc gttatcatcg aacgggacgg cgcagtcctc
     2461 aataccgatt gggcggatta cgaccagtcg ggcgacaccg ttaccgtagg cgaccggttc
     2521 gccctccaac aggacggtac gctgattcgg ggcgaaaccc tgacctacaa tctcgatcag
     2581 cagaccggcg aagcgcacaa cgtccgtatg gaaaccgaac aaggcggacg gcggctgcaa
     2641 agcgtcagcc gcaccgccga aatgttgggc gaagggcgtt acaaactgac ggaaacccaa
     2701 ttcaacacct gttccgccgg agatgccggc tggtatgtca aggccgcctc tgtcgaagcc
     2761 gatcggggaa aaggcatagg cgttgccaaa cacgccgcct tcgtgttcgg cggcgttccc
     2821 cttttctata cgccttgggc ggacttcccg cttgacggca accgcaaaag cggactgctc
     2881 gtcccgtccg tatctgccgg ttcggacggc gtttcccttt ccgtccccta ttatttcaac
     2941 cttgccccca acttcgatgc cactttcgcc cccggcatta tcggcgaacg cggcgcgacg
     3001 tttgacggac aaatccgtta cctgcgtccc gattacagcg gacagaccga cctgacctgg
     3061 ctgccgcacg ataagaaaag cggcaggaac aaccgctatc aggcaaaatg gcagcaccgg
     3121 cacgacattt ccgacacgct tcaggcgggt gtcgatttca accaagtctc cgacagcggc
     3181 tactaccgcg acttttacgg cggcgaagaa atcgccggca acgtcaacct caaccgccgc
     3241 gtatggctgg attatggcgg cagggcggcg ggaggcagcc tgaatgccgg cctttcggtt
     3301 cagaaatacc agacgctggc aaaccaaagc ggctacaaag acgaacctta cgccatcatg
     3361 ccccgccttt ctgccgattg gcataaaaac gcaggcaggg cgcaaatcgg cgtgtccgca
     3421 caatttacac gcttcagcca agacggccgc caagacggca gccgactggt cgtgtatccc
     3481 ggtatcaaat gggatttcag caacagctgg ggctacgtcc gccccaaact ggggctgcac
     3541 gccacttatt acagcctcga cagtttcggc ggcaaagcat cccgcagcgt cgggcgcgtt
     3601 ttgcccgttg tcaatatcga cggcggcaca accttcgaac gcaatacgcg cctgttcggc
     3661 ggcggagtcg tgcaaaccat cgagccgcgc ctgttctaca actatattcc tgccaaatct
     3721 caaaacgacc tgcccaattt cgattcgtcg gaaagcagct tcggctacgg gcagcttttc
     3781 cgcgaaaacc tttattacgg caacgaccgc atcaacgccg ccaacagcct ttccaccgcc
     3841 gtgcagagcc gtattttgga cggcgcgacg ggggaggagc gtttccgcgc cggtatcggt
     3901 cagaaattct atttcaagga tgatgcggtg atgcttgacg gcagcgtcgg caaaaatccg
     3961 cgcagccgtt ccgactgggt ggcattcgcc tccggcggca taggcgggcg tttcaccctc
     4021 gacagcagca tccactacaa ccaaaacgac aaacgcgccg aacattacgc cgtcggcgca
     4081 ggctaccgcc ccgcccccgg aaaagtgttg aacgcccgct acaaatacgg gcgcaacgaa
     4141 aaaatctacc tgcaggcgga cggttcctat ttttacgaca aactcagcca gctcgacctg
     4201 tccgcacaat ggccgctgac gcgcaacctg tctgccgtcg tccgctacaa ctacggtttt
     4261 gaagccaaaa aaccgataga aatgcttgcc ggtgcagaat acaaaagcag ttgcggctgc
     4321 tggggcgcgg gcgtgtacgc ccaacgctac gttaccggcg aaaacaccta caaaaacgcc
     4381 gtcttttttt cacttcagtt gaaagacctc agcagcgtcg gcagaaaccc cgcaggcagg
     4441 atggatgtcg ccgttcccgg ctacatcccc gcccactctc tttccgccgg acgcaacaaa
     4501 cggccctgac cgtcggaaac ccggcaggag caccgttcct gcacaagata gcattccacc
     4561 gacaacccca aacccgccat caaaggcagg attcaaacga taaggaaaga atgatgaaaa
     4621 tcaaagccct gatgattgcc gccgcattgc tggcagcagc cgatgtccac gccgcaccgc
     4681 aaaaggcaaa aaccgcgccc gccaaagcgg tcaaagctgc cgccacggcg caaaaagaag
     4741 ccgcacccgc acaacagcag ggcggtatcc gcttttcaga cggcattgcc gccgttgccg
     4801 acaacgaagt catcaccaac cgccggcttg ccgaagccgt tgccgaagcc aaagccaccc
     4861 tgcccgaaga cgcgcagata agtgaatccg agctgtcccg acaggtgctg atgcagcttg
     4921 tcaaccaatc cctgattgta caggcgggca aacgccgcaa cattcaggca agtgaagcgg
     4981 aaatcgatgc cgtcgtcgca caaaatcccg ccctcaaaaa cctcagcccc acccaacgcc
     5041 gcgagcttgc cgacaacatc attgccgaaa aagtccgcca gcaggcagtg atgcaaaaca
     5101 gccgcgtgag cgaagctgaa attgatgcct tcctcgagca ggcgcaaaaa caaggcatca
     5161 ccctgcccga aggcgcaccg ttgcgccaat accgcgccca acacatcctg attaaagccg
     5221 acagcaaaaa cgccgccgtc ggcgcggaaa gtaccatccg caaaatctac gaccaggcgc
     5281 gcaacggcac ggatttcgca ggtttggcac gccgatattc ccaagacgca agcgcgggca
     5341 atggcggcga cttgggctgg tttgccgacg gcgtaatggt tcccgccttt gaagaagccg
     5401 tccacgcgct caaacccgga caggtcggcg cgcctgtccg cacccaattc ggctggcaca
     5461 tcatcaaatt gaacgaagtg cgcgatgccg gcacgccgga ggaacgtatc cgcaattccg
     5521 tgcggcaata catcttccaa caaaaagccg gacaggcaac cgtcaacctg ttgcgcgacc
     5581 tgcattccgg cgcgtatgtc gacatccgct aaggcggttt gaatccaaaa gtcataccga
     5641 tcgggcaaaa gtccgggcgg tatggctttt ttgaatttcg agttactttt acaccgccat
     5701 tcgtcattcc cgcgcaggcg ggaatctagc aacgaaaagt aacaggaatt tatcgggaat
     5761 ggccggagtt taaaggtctg gattcccgcc ttcgcgggaa tgacgattca gaagttacac
     5821 gaaactcaaa aaaaaaaacg aaaccgaacg aaccggattc ccgcttttac gggaatgacg
     5881 gcatatcggt ttccgtgagg acaaacctag attcccgcct gcgcgggaat gacgggattt
     5941 tagattgcgg gtatttatcg ggaatggcga tttaaaaatt acctaaaact aaacttaaaa
     6001 gcaactgaaa cttaaaaaac taaaatttcg cctgcgtggg aaggatggga tttcagattt
     6061 ctgtttttgt gggaatttgc cgtatcgggc ttcagacggc attgcctgcc gttttgcccc
     6121 cgggtgcggc tgccttgatg tagttgagcg agacaaactg cttctcggca tccaattcgg
     6181 tgattttaaa caatgcctgc gatttgggca gcgcgtcaaa cggaataccg gtcgcgcgcg
     6241 ttgtcagtgg cagaccttcg atgcggacga ggtcttcttt cagaatcgtc gcggtcagtt
     6301 cgctgatgcc ttgttgttgc aggtacacga ggctccagta ggcttccatc tgccgttgga
     6361 agtcggcgta ggcggcgtag gcggtgtcga aatcgcgcag ggcggcgaag agcgcggcat
     6421 cgcttttttg atacagcggc tcggcagtgt cgtcgatcag gctgatcagc tgcttttggt
     6481 tgatgtagtc ggcggcgcgg cgcagcggcg aggtaaacca gccgtaatgc tgcacgccca
     6541 tgccgatgtg cggctcggat tgggtgctca tgcgcacttt gcctgccggt tggacgcgga
     6601 aaatgccggg cagcccgttt tcgtctagca tttgcgccca agtgctgttg gcaagaatca
     6661 tcatctcgct gaccagcgta tcgatgggcg aaccgcgttc gcggcggacg acggagacgt
     6721 tgccttcttc atccaattcg atgctgtaat cgtattgcgg cgcgcggtcg ggttcgtatt
     6781 tgccgcgcgc tttttgcagg gcggtggcga attgatagaa ccaaatcagg tcttgatggt
     6841 gggcgaacat catttcgcct gcttggtcca agccggtttc tgcgttgaaa tgcggctcga
     6901 tggcttggat acgcaggttt gcggcgatgt tgacggcttc gattttgcag gtcggctcgc
     6961 cgacgttgaa ctcgccgtcc acatcgaaat aaatgctgac ggcagggcgg tatgcgcctg
     7021 catcgaggct gaacgcggta atccagtttt cgggcagcat cgtgattttg ccgccgggga
     7081 aataaaccgt actcaaccgc tgcataatga tttgttccat tccgccgcct tgtcggacgg
     7141 caagcgacgg cgcggcgatg tggatgccga cgcgcttcgt gccgttgccc aaatcggtca
     7201 ggctcaaggc atcgtccact tcggtggtcg attcgtcgtc aatggaaaag gcggtaacgt
     7261 cggccttggg caggtcgggc atttcgggaa gggagaggtc ggggaaacct gttcctttgg
     7321 ggaagtattt gatttcaaag ccttcttgca ggtattgcgg aatggacgta atgccgcccg
     7381 tttttttcgc cagttcgtag gcggaggttt tcagtgcgtc ggcggctttg gtaaaggctt
     7441 tgtaggtcag cgactgtttg tcgggcgcgt gtaagatggt tctcaaatcc gccgcgattt
     7501 cagacggcat ctcgccgcgt ttcaaggctt ccgcccaagc gtcgatttgc gcgtcttgct
     7561 gttttttgcg ttcgatggcg gcaagcgctt gtttcaaagt ttcttcgggc gcggctttga
     7621 acacgccttt ggcttttttg tagaaataca ccggcgcggc gtacagcgca atcaaagtcg
     7681 ccgccagctc ggttttggtc ggcgcatggc cgtaatattc ttcggcgatg gcttcggcgg
     7741 taaactcctc ttcgccgcat acttcccaca ataaatcggt gtcgatgtcc gccgcctgcg
     7801 cctgagcgtt ttccaaaaac gccgccatat cgccgtcaaa ctcggcaaag acattgtttg
     7861 ccttcacttt ggtgcgtttg ccgtgttggg tatcgacttg gtaggtagcg tcgttttttt
     7921 ggacgacgac ggcaactttg aactggccgg actcttcgta aaaaatattc atttttcgga
     7981 tttttctgtg gaaactcaag cgggcgattt tagcagatta ccgaaaatgc cgtctgaaaa
     8041 aaggttgggg gaaggcgggt gcggctttgc ggtgcttgcg ttgcgttttt gacggggtac
     8101 aatcgccgtt tttgaacgga ggacggaatg gagaatgtaa accgcgtgcc ggagcaggca
     8161 cgttatgatg ccgaacgcag gcaggcagac gaagcattgg cggacgtgtt tccggcagtc
     8221 agtattttcg gcagcgcgcg cacgccgcag gatcatgcgg attatgcgtt tgcctgccgt
     8281 ctggcgcggc ggctgtcgga ttcgggtatt gccgtcattt cgggcggcgg gccggggatt
     8341 atggaggcgg caaacaaggg cgcgtttgcc gggaagtcgg tttcggtagg gctgaacatt
     8401 gccctgccgc acgagcagaa accgaatccg tatcaggaca tcgccttgcg gttttcccgt
     8461 tttgccgaac gcaaggcagt gtttttccgc tattcccaag catatgtcgt gatgccgggc
     8521 ggcttcggga cgctggacga attgtttgaa atcctgacct tggtgcagac gggcaaagtg
     8581 ccgccgcgtc cggtcgtttt ggttggaaag gcgttttggt cgggcttggc ggagtggata
     8641 aacgcgcagc ttttggcgcg cggtatgatt tccgaagggg ccgcttcttt gttctccata
     8701 tcggacgacg aagacgaaat cattgcgtat ctgtcggaac acgggcttca gacggcatag
     8761 cgtcctgaga gtgatgtata attgcaaaca atttgtcaat ttttgatgtc ttttccgaac
     8821 aggatgccga tatgatcaac cccatcgcct cgctttcccc cctagacggc cgttatgccc
     8881 aatccgttga agcattgcgc ccgatttttt cagagtacgg actgatgaag gcgcgcgtca
     8941 aagtcgaatt aaactggctc aaagccctgg ccgccgcgcc ggagattgcc gaagtgccgc
     9001 ccttcagtgc cgaaacgctt gccgaaatcg acaaagtcat cgaaaacttt tcattagaag
     9061 acgcggccgc cgtcaaagcc atcgaagcca cgaccaatca tgatgtcaaa gccatcgaat
     9121 attggctgaa aaaacgtttt gccgaagtgc cggaagtcgc cgccgtgagc gagttcatcc
     9181 acttcgcctg caccagcgaa gacatcaaca acctgtccca cgccctgatg ctgcaagagg
     9241 cgcgcgaaac cgtcatcctg cccaaacttg ccgaaatcat cggcaaactc accgctatgg
     9301 cgcacgacct tgccgccgtc ccgatgatga gccgcaccca cggccagccc gccacaccga
     9361 ccactttggg caaagaaacc gccaatgtcg tgtaccgcct gcaacgccag ttcaaaatcc
     9421 ttcaggcgca agaattcctc ggcaaaatca acggcgcggt cggcaactac aacgcccaca
     9481 tggccgccta tcccgatgtg gattgggaaa cacactgccg caacttcgtc gaaatcagcc
     9541 tcggcctgac cttcaacccc tacaccatcc aaatcgagcc gcacgactat atggcggagt
     9601 ttttccaaac cctcagccgt gtcaacacca tcctgatcga ctttaaccgc gacgtttggg
     9661 gttatatttc attgggttat ttcaaacaaa aagtcaaagc gggcgaagtc ggttcttcca
     9721 ccatgccgca caaagtcaac cccatcgact ttgaaaactc cgagggcaac ctcggcatgg
     9781 caaacgccgt attgggcttt ttgtccgaaa aactgccgat ttcccgctgg cagcgcgacc
     9841 tgaccgacag caccgtattg cgcaatatgg gcgtgggcgt gggctatgcc gtgttgggtt
     9901 tcgccgccca cctgcgcggt ctgaacaagc tcgagccgaa ccctgccgcg cttgccgccg
     9961 atttggatgt cacttgggag ctgctcgccg agccgattca aaccgtaatg cgccgttacg
    10021 gtgtcgccaa tccttacgaa aaactgaaag acctgacgcg cggtaaaggc ggcatcacac
    10081 ccgaagtgct gaaaggcttt atcggatcgc tggaaattcc cgccgaagcc aaagccaaat
    10141 tgcttgagct gacccccgcg ctgtatgtgg gcaaggctga agcgttggcg aaacggattt
    10201 gagcgtttac tgaaaccgat gccgtccgaa cgcgcgttca gacggcattt ttaagataac
    10261 gggacatacg gggcgatatt gatgcaagct gtccgataca ggcctgaaat tgacggattg
    10321 cgggccgtcg ccgtgctatc cgtcattatt ttccacctga ataaccgctg gctgcccgga
    10381 ggattcctgg gggtggacat tttctttgtc atctcgggat tcctcattac caacatcatt
    10441 ctttctgaaa tacagaacgg ttctttttct ttccgggatt tttatacccg caggattaag
    10501 cggatttatc ctgcttttat tgcggccgtg tcgctggctt cggtaattgc ctctcaaatc
    10561 ttcctttacg aagatttcaa ccaaatgcgg aaaaccgtgg agctttctgc ggttttcttg
    10621 tccaatattt atctggggtt tcagcagggg tatttcgatt tgagtgccga cgagaacccc
    10681 gtactgcata tctggtcttt ggcggtagag gaacagtatt acctcctgta tcctcttttg
    10741 ctgatatttt gttacaaaaa aacaaaatcg ctacgggtgc tgcgccatat cagcatcatc
    10801 ctgtttttga ttttgactgc ctcatcgttt ttgccaagcg ggttttatac cgacatcctc
    10861 aaccaaccca atacttatta cctttcgaca ctgaggtttc ccgagctgtt ggcaggttcg
    10921 ctgctggcgg tttacgggca aacgcaaaac ggcagacggc aaacagcaaa tggaaaacgg
    10981 cagttgcttt catcactctg cttcggcgca ttgcttgcct gcctgttcgt gattgacaaa
    11041 cacaatccgt ttatcccggg aatgaccctg ctccttccct gcctgctgac ggcactgctt
    11101 atccggagta tgcaatacgg gacacttccg acccgcatcc tgtcggcaag ccccatcgta
    11161 tttgtcggca aaatctctta ttccctatac ctgtaccatt ggatttttat tgctttcgcc
    11221 cattacatta caggcgacaa acagctcgga ctgcctgccg tatcggcggt tgccgcgttg
    11281 acggccggat tttccctgtt gagttattat ttgattgaac agccgcttag aaaacggaag
    11341 atgaccttca aaaaggcatt tttctgcctc tatctcgccc cgtccctgat acttgtcggt
    11401 tacaacctgt attcaagagg gatattgaaa caggaacacc tccgcccgtt gcccggcgcg
    11461 ccccttgctg cggaaaatca ttttccggaa accgtcctga ccctcggcga ctcgcacgcc
    11521 ggacacctgc gggggtttct ggattatgtc ggcagccggg aagggtggaa agccaaaatc
    11581 ctgtccctcg attcggagtg tttggtttgg gtggatacga cactggcaga caacccgtta
    11641 tgtcgaaaat accgggatga agttgaaaaa gccgaagccg ttttcattgc ccaattctat
    11701 gatttgagga tgggcggcca gcccgtgccg agatttgaag cgcaatcctt cctaataccc
    11761 gggttccaag cccgattcag ggaaaccgtc aaaaggatag ccgccgtcaa acccgtctat
    11821 gtttttgcaa acaacacatc aatcagccgt tcgcccctga gggaggaaaa attgaaaaga
    11881 tttgctataa accaatatct gcgccccatt caggctatgg gcgacatcgg caagagcaat
    11941 caggcggtct ttgatttggt taaagatatt cccaatgtgc attgggtgga cgcacaaaaa
    12001 tacctgcctg aaaacacggt cgaaatacac ggacgctatc tttacggcga ccaagaccac
    12061 ctgacctatt tcggttctta gttatatggg gcgggaattc cacaaacacg aaagcctgct
    12121 caagcattcc cgaggcggcg cattgtagta gcctgccttc ttgccggata ttgcctttgg
    12181 cagcctatgc cgctgtttgc cctttgaggc ggcttttatt ttgccgtttt gaggccgggg
    12241 tcgggaataa ccgttttttg atgattttcc ctccccggct gtgtcatcaa atccctagtt
    12301 gcctttccaa actctccacc agattgtcat ccagttccaa agcctgcgac aggcgggcga
    12361 ggaagacggt ttctttccgc gacaaatcgg cacagaccaa ccttgccgcc agataggtct
    12421 ccgccgccaa cgcctcatcg ttgccgacgg cggcggcgat gtccccgatg cttgcgggca
    12481 ggcggtattc ggcggcgagc catgcggcag tttcggggtc tgtgccgctt tcctgttcga
    12541 tagtccggcg ttcggcttcg tctatcatgc cgtctgaagc ggcggcggct atcatggtac
    12601 gcaatacggt gcggctgtat gtttcttcgg tttttccggc aggttggaaa tcgctttgtg
    12661 ttacggttgc ccgccctttg ttttgctgcc acatctgata gccccggtag gcgaggtagc
    12721 ccaaagcggc ggtcgaaccg attttggtga tggttttgcg gtttttaccg ttcagcagca
    12781 tggaggcgac accggcaacc agcgcgcctc cgccgaatga attgagcggg ctgccggaga
    12841 atgtgctgcc ttttttttga accgtgctta agacttggtt gagcaaccgg gtgaagttca
    12901 tgatttttcc tttctgttgc gggaagggcg gtatgtttac cctatccttt taaacggtgg
    12961 caggccggcc aataattgtt ggccgtaccg ctgcgttttg atgcggttgt cgaggatggt
    13021 tacgcggccg tagtcttgtt cggtgcggat gaggcggccg acggcctgga tgagtttgat
    13081 gccggcttcg gggacggtga tttcgatgaa ggggttgccg ccgcgttgtt ctatccaacg
    13141 gttttgggtt ttttcgatgg ggttgtcggg catggcgaag gggagtttgg cgatgatgac
    13201 ttgcacgcag gcggtgccgg gcaggtcgag cccttcggcg aagctgtcga gtccgaagat
    13261 gatgctggct ttgccttctt ctatggcttg gtggtgtttt tgcaggagga cggctttggg
    13321 taattcgcct tgtacgagca agagcggcag gtagtctccg ggcaggtgca gggcgacatc
    13381 ctgcatttgt ttgcgcgagg aaaacaggac gagtgtgccg atggcttcgg tgggcgaaat
    13441 cagcttgggc agccattcga tgacggcggc ggtgtgggct tcggggtctt tggggctggc
    13501 gtatatggag gggatgtaga gttcgccctg tttttcaaag tcaaaggggc ttttgagggc
    13561 gagggtggtg gtttcgggca gccacagcag gccggtttgg cgcaaaatca agttgaagct
    13621 gccgagcgat tgcagggtgg ccgaagtcag caccgcgcct gccgcgcgcc gccacaggct
    13681 gttggcaagg tgggacgcgc tgctgatggg gctggcattg aaaatatagt cgtttttgtc
    13741 gtcggcacgg cgggctatcc atttcgccaa cggctcctcg ccctcaatgg aaacagtgga
    13801 gagcaaatcc caaaccgcgc tgatttgttc gatacgggcg ataaaaaggc cgaactcgcc
    13861 ggtcaggcgg tcgatgagcg caccgtcctg ttctttttcg cggcgtgcgg cggaaagcgc
    13921 atcgttcagc ccgacaacgt gtttgagcag gctgcgtgca gcaatggccg tattggaaac
    13981 ggtggtttcg aggccttcgg ggattttgcc gtcttcccac agccaagtcg gttcgctgtt
    14041 ggttcgtctg tcgttttcag acagccccat acttaaagac ggctcttccg ccaaatggaa
    14101 ttgccattcg tgcaggctgt cgagcaagga tgcggcggct tcgtcggcaa ggttggcaag
    14161 ttcggcttta tcggtaagcg cggcaatttt gccggtaagc tgcggcagtt tttccagcgt
    14221 ccaaacggca atattccatg aatgttcggc ggcaaaacgg ctgagggctt ttttgggcag
    14281 gtggtgcgct tcgtctatgc aatagaaact gttttcgggc gcaggcagaa tcacgccgcc
    14341 gcccatgctg atgtcggcaa gcagaagatc gtggttggca acaacgacat cgacggtttc
    14401 caacatatcg cgtgccaggt aaaaagggca ttccggacgg ttgggacagg cggctttcag
    14461 gcagccgtgg cggtcgttgg tcactttgag ccaaatcgcg tcatcgattt tttccggcca
    14521 agcgtcgcgg tcgccgttga accgtcgggc ggaaaattcg tcggcgatgt cgcgcagcag
    14581 cttcaattct tccggcttgg gtttgctgtc ccacaagacg gcgggggctt caaagccgag
    14641 caggttttgc tgggcgttgc tttgcgtcag ttgatagagt ttgtaggggc agagatagcg
    14701 gccgcgccct ttggcaagtg cgaaggtcag ttccaaaccg cttttttcga ccagaaacgg
    14761 caggtcgcgg tctaccaact gctcctgcaa ggcaaccgtc gcgctgctca caatcagccg
    14821 cttgccgcgt gtttgcgcca tgatgccgcc cgcaagcagg taggcaagcg atttgcccac
    14881 gccggtcggc ccttcgatta cggcaatgct ttcgccctcg cgcttgggcg gctcgccgcc
    14941 ttcttcgcgc gtcaacgtcc gcgaaaaagc gttggcaacc gccgcaatca tttcccgctg
    15001 cgaagcacgc ggacggaaac cgggcaggtt tttgccgata ttttggtaat ggtcgcggat
    15061 ggcgtttttt tctaaatcgg tgagcatagt atcggtgggc ggatgatttg gggtcatttt
    15121 aacattgcgc ggatgcggta cggtatcgtt gtcggacggt ggttcgggca tatctgatta
    15181 ttgaaagtaa aaaaaaacgg aagatattgg gtcatccgta caaaatgccg tctgaagcgg
    15241 gaaacgggct tcagacggca ttgccgcgct tatacgccgg cgacggggcg ggcgggatag
    15301 ccgtgggtat cgaccgaaca ggcaaaccgc caaggcgtgt ggacggtgtc ggcggacagg
    15361 tgggcgagtt cgggaatgtg ccgtttgata aaagtaccgt ccgggtcggt acggtatgag
    15421 gcggcggcaa tgtcggggca ggtgtgccgt gaggcggcaa gccgccagtt gccttggttg
    15481 attgccgcat cgaaatcggt cagctgccgg gcaaaccata tctcgccttc gcggcggggg
    15541 aggtttaaaa cgtggcagaa aaaatccgcg ctcaagcgtc tcagggcggg gtggaggctg
    15601 ccggttttgt gcaaacagcg catcgcggca tcgataatcg gaatgccggt ccggccctgc
    15661 tgccaaagcg tcaggcgcag ggtgtgttcc ggattgccgt ctgaagggtc gtcatccgcg
    15721 tgctgcaagg caagttgaag gaaaaaatcg cggcggatga tgttgtccgc ccacgcgttc
    15781 agacggcgtt cgaggctttc ccgcgcgagc aggcgcggcg agatgcagcc gacactcaaa
    15841 tacgcgccca tcagcgaagt gtttttgcgc gaagggaaat cctttaaaac ggagtaggaa
    15901 gccgcctgtt cgagaaaccg ccgccactgc cgccaagccg ccgtttcgcc gccgttttgc
    15961 ggcaggaaga tgccgtctga aagcgcggca ggcggcgggg cgggcaggtt ttcggggaag
    16021 ggttggcggt atgccgcgaa taggtccgaa ccggcggggg gctgcttgga aaagcggtcg
    16081 agccatactt cgcggtagcg gttgaaatcg gtgtgtgccg taccgttgtc tggtgtcagg
    16141 tcggttttgc cgaaaacgga acggtcgttg acgaaggtta acgcgatgcc gtgtttgtcc
    16201 aattcgtgcc aaagggcgtt gtcggcgagt ttgtcggcaa aagtatggga ttcgtcggcg
    16261 atgacggcgc ggatattgag gcggacggcg agccggacaa gcccggcagg cgatgccgcc
    16321 gtgtagagcg ggatgccgcg ccttgccagc ccttgggcga gttcggcggc ggattggcgg
    16381 tagaacgcgg cgcggcgcgg gttgtctgtt tcggcatcgt caatccaaat gccgataacg
    16441 ggcgaacctt cggcaacggc ggcgcataag gcggcgttgt cgcggatgcg gaggttttgg
    16501 cggaaccaga cgagcgtgtg cgcggcgcgc gtgtccgcat aaaggaggag ggcggtttca
    16561 gacggcattt cggcagcctt tcctgctggc gattttttcg ttcagaaaat cgatgaaact
    16621 gcggactttc gcactcaaaa atgccctgtc cgcataaacg gcgttcagcc ggtcggtcgg
    16681 gacggcgtat ccgggcagca gcctcaccag cgggccgcag cgcaaatcgt gttccgccgc
    16741 ccaaagcggc tgataaccga tgcacgcgcc cgccttaatc atttcgcgca tcatcagcgt
    16801 gttgtcggta cggatgacgg gggtcagttc aagccggtat ttcttgccgt ccgatttgcg
    16861 ggtgaggtcg agtttctgct ggttggtgta ggtcggcagg acggcaggca gccccgccac
    16921 ttcttccggt gtttccggca cgccgttgcg cctcaggaag tcgggcgagg caagcagggc
    16981 aaattcgatt tccgccagcg ggcgcgcaat cagcgaaggg gaaagggttt gggaaacgcg
    17041 caacgccaaa tccacgcctt cggcaatcaa atcgacgtgg cggttgtcca aaatcagttc
    17101 caacgccact tcgggataac gttcgcggta ttccgccagc cagttgcata tctggctgcc
    17161 ggcaaaccac agcggcatcg ttacgcgcag cagcccctgc ggtttttccg tccccccggc
    17221 ggctttttgc gcggcatcgt cgagcgtgtc gagcgcgtaa ctgcattgcc ggtagtattc
    17281 ttccccggct tcggtcaggc tgaggttgcg gctgttgtgg tgcaggagtt tggcttggac
    17341 ggtgttttcc aagtgactga cgtgtttgct tgccattgcg gtggagatgc cgagcgcgtc
    17401 ggcggcgcgg gtgaagccgc cgctttggac gacttggcgg aaaactttga ggctgaacag
    17461 ggtgtccata ttttcttgtg tggaaaagtt gtatcaataa aagcagtata tatttgaaaa
    17521 agggaaacat ctatactctg ccgcctgaaa tgaagacaaa tatcaaagga gcttttatgt
    17581 ccgattgctg caaccgtatc caaccggttt tgctttctgt tttgcgtatc gtaaccgcct
    17641 acctgttttt gttgcacggt acgtcgaaaa tcttcgcctt ccccattgaa atgggcagcg
    17701 gttcgcccgg cgggctgttg ctgcttgccg gtattttaga aattgtcggc ggcattttgc
    17761 tggtgttggg cctgtttgcg cgccctgccg cgtttgtttt gtccggccag atggcggttg
    17821 cctattttat ggcgcacgct tccggaaatg ctttgttccc gattgccaac ggcggcgagt
    17881 ccgcagtgct gttctgcttc gtatttctct atatcgcggc agcgggcggc ggagcatggt
    17941 cgctggacag gctgtttttc aagcgtaaag cctgaaccgg actgcctaaa gtgtattttg
    18001 ttgaatgttt ttgaggaaaa gaaatgagcc gtcaatccct gcaacaggct gccgaaagcc
    18061 gccgttccgt ttattcgtta aataaaaacc tgcccgttgg caaagatgaa atcgtccaaa
    18121 tcgtcgaaca cgccgttttg cacacacctt tttcgttcaa ttcccaatct gcccgcgtgg
    18181 tcgtgctgtt tggcgaagag
//
LOCUS       DBJGGI010000038        18095 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.038, whole genome shotgun sequence.
ACCESSION   DBJGGI010000038 DBJGGI010000000
VERSION     DBJGGI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18095
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.038"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..241
                     /locus_tag="ACSIGJ_001306"
     CDS             <1..241
                     /locus_tag="ACSIGJ_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330158.1"
                     /translation="ESYKVKFSEYLFENKTENNSDGNPTANYFFEIIDDSYMDWLKEE
                     SFGFFEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(384..497)
                     /locus_tag="ACSIGJ_001307"
                     /pseudo
     CDS             complement(384..497)
                     /locus_tag="ACSIGJ_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            513..1007
                     /locus_tag="ACSIGJ_001308"
     CDS             513..1007
                     /locus_tag="ACSIGJ_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330159.1"
                     /translation="MENRFEKIVKDIFSFTQFVSEAENKIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKDLFAEEVPENNIVCDISHFLHFADRA
                     EADIPNQSRLYAEYWWEMETFKMSVLKDWERSGRPLDWEKWQRQYQSEAFELVKNLNR
                     SKQE"
     gene            1127..2260
                     /locus_tag="ACSIGJ_001309"
     CDS             1127..2260
                     /locus_tag="ACSIGJ_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330160.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNTFVAKLARLFRNADRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAVRLSRL
                     KEKAKWFHVTEQEHGEEVWLDYYIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADMQTYHAQQTWYLDGGRI
                     IREEKQGDRLPDFPLNLEDLEKEVSRYAEAAARRSGGRRGLSH"
     gene            complement(2763..3038)
                     /locus_tag="ACSIGJ_001310"
     CDS             complement(2763..3038)
                     /locus_tag="ACSIGJ_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HHQ1330161.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAIHTAGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALKQEIAVKKLTAAQKRQLWEQGEKMPSET"
     gene            complement(3035..4318)
                     /locus_tag="ACSIGJ_001311"
     CDS             complement(3035..4318)
                     /locus_tag="ACSIGJ_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHQ1330162.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFASGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAHEPVLPPSVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAITVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWMG
                     YVPFFRLCFILALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(4467..5885)
                     /gene="glnA"
                     /locus_tag="ACSIGJ_001312"
     CDS             complement(4467..5885)
                     /gene="glnA"
                     /locus_tag="ACSIGJ_001312"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HHQ1330163.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGPAFDGSSIGGWKGIEASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEGLGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            6196..7005
                     /gene="aroE"
                     /locus_tag="ACSIGJ_001313"
     CDS             6196..7005
                     /gene="aroE"
                     /locus_tag="ACSIGJ_001313"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HHQ1330164.1"
                     /translation="MHALPRYAVFGNPAAHSKSPQIHQQFALQEGVDIEYGRICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSDRALAAGAVNTLVWLEDGRIRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSPKIFRDCRLAYDMVYGEA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFKPDIRPVIEHMKAL"
     gene            7008..7709
                     /gene="mtgA"
                     /locus_tag="ACSIGJ_001314"
     CDS             7008..7709
                     /gene="mtgA"
                     /locus_tag="ACSIGJ_001314"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HHQ1330165.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWMPYKRISTNLKKALIASEDVRFAGHGGFDWGGIQNAIRRNR
                     NSGEVKAGGSTISQQLAKNLFLNESRNYLRKGEEAAITAMMEAVTDKNRIFELYLNSI
                     EWHYGVFGAEAASRYFYKKPAADLTKQQAAKLTALVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(7983..8058)
                     /locus_tag="ACSIGJ_001315"
     tRNA            complement(7983..8058)
                     /locus_tag="ACSIGJ_001315"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8023..8025),aa:Phe,seq:gaa)
     gene            complement(8249..8983)
                     /gene="lptB"
                     /locus_tag="ACSIGJ_001316"
     CDS             complement(8249..8983)
                     /gene="lptB"
                     /locus_tag="ACSIGJ_001316"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HHQ1330166.1"
                     /translation="MSANNSRLVVQNLQKSFKKRQVVKSFSLEIESGEVIGLLGPNGA
                     GKTTGFYMIVGLIAADAGSVMLDGRELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRNPAPSLSGGERRRVEIARVLAMKPR
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRAVYLGENFKY"
     gene            complement(9054..9584)
                     /gene="lptA"
                     /locus_tag="ACSIGJ_001317"
     CDS             complement(9054..9584)
                     /gene="lptA"
                     /locus_tag="ACSIGJ_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HHQ1330167.1"
                     /translation="MIQKICKLFVLIVIFATSPAFALQSDSRRPIQIEADQGSLDQAN
                     QSTTFSGNVIIRQGTLNISASRVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVTYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTINGSTKSGAKSASKT
                     GRVSVVIQPSSTQKTE"
     gene            complement(9565..10146)
                     /gene="lptC"
                     /locus_tag="ACSIGJ_001318"
     CDS             complement(9565..10146)
                     /gene="lptC"
                     /locus_tag="ACSIGJ_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HHQ1330168.1"
                     /translation="MKVRWRYGIAFPLILAVALGGLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPENSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGRRQAGKVETEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYNHKTGMLNFSSKVKAAIYDTKDM"
     gene            complement(10143..10679)
                     /locus_tag="ACSIGJ_001319"
     CDS             complement(10143..10679)
                     /locus_tag="ACSIGJ_001319"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HHQ1330169.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDDGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQA
                     GLEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIIRAKGTLGAALNGYIK"
     gene            complement(10745..11719)
                     /locus_tag="ACSIGJ_001320"
     CDS             complement(10745..11719)
                     /locus_tag="ACSIGJ_001320"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HHQ1330170.1"
                     /translation="MAENEKYLDWAREVLHTEAEGLREIAAELDENFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVAAISNSG
                     ESDEITAIIPALKRKDITLVCITARPDSTMARHADIHITASVSQEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALIHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKGAIVSMSEKGLGMLAVTDGQGCLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLTGALNMHDLLAARIV"
     gene            11816..12871
                     /gene="tal"
                     /locus_tag="ACSIGJ_001321"
     CDS             11816..12871
                     /gene="tal"
                     /locus_tag="ACSIGJ_001321"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HHQ1330171.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKRQNLSPKQRYETMAVADVRAACDVCLAEHESTGGKTG
                     FVSLEVSPELAKDAQGTVEEARRLHAAIARKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIQVVASFFISRVDSALDATLPDRLK
                     GKTAIALAKAAYQDWEQYFTAPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGVHTVNTVPDATLKAFIDHGTAKATLTESADEARARLAEIAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            12883..13017
                     /locus_tag="ACSIGJ_001322"
     CDS             12883..13017
                     /locus_tag="ACSIGJ_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330172.1"
                     /translation="MPSERLSDGIALLTGHSAAYQGSALKILMGKKLMHDITRQHKRK
                     "
     gene            13022..13312
                     /locus_tag="ACSIGJ_001323"
     CDS             13022..13312
                     /locus_tag="ACSIGJ_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330173.1"
                     /translation="MAKALEIISPDEIYSDLIFKDPVPPHTIYTELMKLVGRNAGNER
                     IADRAFDFFSPALMDDSATEEQYNALYDLTLLEEPGMELDKDEITALINSLK"
     gene            13329..14216
                     /gene="gluQRS"
                     /locus_tag="ACSIGJ_001324"
     CDS             13329..14216
                     /gene="gluQRS"
                     /locus_tag="ACSIGJ_001324"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HHQ1330174.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVTYQSRRYALYEETLYRLKTAGLVYPCHCNRK
                     DWQAGARRGTDGFVYNGRCRHPGQRPALQGKQPSWRIRVPDRDIGFSDGIVGSYTQNL
                     ARDIGDFVLFRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDSNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAITAP"
     gene            complement(14286..15302)
                     /gene="dusA"
                     /locus_tag="ACSIGJ_001325"
     CDS             complement(14286..15302)
                     /gene="dusA"
                     /locus_tag="ACSIGJ_001325"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HHQ1330175.1"
                     /translation="MINKELIEHKGKRRLCVAPMLDWTDRHYRYLARQITRNAWLYSE
                     MVNAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGC
                     PSPRVQKGAFGACLMNEVGLVADCLNAMHDAVGIPVTVKHRIGVDRQTEYQTVADFVG
                     TLRDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPELEIIINGGIT
                     TNEAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDNRAPIEYADLVQRLYTYSQV
                     QIQAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGGLIFEAWKEVERA
                     NTRE"
     gene            15422..16576
                     /locus_tag="ACSIGJ_001326"
     CDS             15422..16576
                     /locus_tag="ACSIGJ_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHQ1330176.1"
                     /translation="MATITKRRNPSGETVYRVQVRVGKKGYPAFNESRTFSKKALAVE
                     WGKKREAEIEAGPELLFKRGKVKMMTLSEAMRKYLNETLGAGRSKKMGLRFLMEFPIG
                     GIGIDKLKRSDFAEHVMQRRRGIPELDIAPIAASTALQELQYIRSVLKHAFYVWGLEI
                     GWQELDFAANGLKRSNMVAKSAIRDRLPTTEELQTLTTYFLRQWQSRKSSIPMHLIMW
                     LAIYTSRRQDEICRLLFDDWHKNDCTRPVRDLKNPNGSTGNNKEFDILPMALPVIDEL
                     PEESVRKRMLANKGIADSLVPCNGKSVSAAWTRACKVLRIKDLRFHDLRHEAATRMAE
                     DGFTIPQMQRVTLHDGWNSLQRYVSVRKRSTRLDFKEAMMQAQSDIKSGK"
     gene            complement(16626..16892)
                     /locus_tag="ACSIGJ_001327"
     CDS             complement(16626..16892)
                     /locus_tag="ACSIGJ_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HHQ1330177.1"
                     /translation="MNISKEQGLLIAYGGRPYIPPREVHKDFFAHIGFNAFKASGVRC
                     ELPFPIFRLSDSRKSEYFVSVRELAKAIADKEEKAKSEYRKFRS"
     gene            complement(17000..>18095)
                     /locus_tag="ACSIGJ_001328"
     CDS             complement(17000..>18095)
                     /locus_tag="ACSIGJ_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HHQ1330178.1"
                     /translation="NTASGISGTAAGAAEQAFFSAAKTANRSASAASANAPPHPDKPE
                     NGRKPMTFQGHNNRKKAGGYAEYITGGSLRRLVAAKVRRYCGEHPGVFDGAAGSGQLE
                     QYIEPSDFRAVEIQAEACKALLQNYPAAKVYNTSLFLYTDGEPQDCTVMNPPFSIKLK
                     DLSEDEKSRIAQEYPWKKSGVADEIFVLKGLENARRFGFFILFPGIAYRKSEQRFREI
                     IGNRLAELNRIQNAFEDTPIEVLLLVIDKDKTDGGCIRELYDCKTDTLLAADTWQIEP
                     DLWQTVQEPAPPKEKEDPVLLEHECRDAAAKRIARELRFSKMVNEIEGWPHAEFDGFC
                     DRLCNLIQAEKYGKKHYFPCSLPLFGGAAG"
BASE COUNT         4187 a         4769 c         4855 g         4284 t
ORIGIN      
        1 tgaatcttat aaagtaaaat tttcagaata tttattcgaa aataaaacag aaaataattc
       61 ggatgggaat cctactgcaa attatttttt cgagataata gatgacagtt atatggactg
      121 gttgaaagaa gaaagttttg gtttttttga aaagaaatat tataaggctt atattttctt
      181 ttttagcgat tctgtaatag aagttatcag ctcgacagaa cctgtatttt attcaaaata
      241 acaaattatc aaacaaaact ctgattaaaa acccaacaat cagaatgccg tctgaagcgg
      301 tattcggctt tcagacggca ttttctccga ttggcacagg atgcaggggc ttgccggctt
      361 gattttgccg aagttgttgg ctcgtatagt ggattaaatt taaaccagta cagcgttgcc
      421 tcgccttgcc gtactatctg tactgtctgc ggcttcgtcg ccttgtcctg atttaaattt
      481 aatccactat aatttggaag aggcagcaac ctatggaaaa tagatttgaa aaaatcgtta
      541 aggatatttt tagttttacc caatttgttt ccgaagcgga aaataaaatt cctaaccaat
      601 cctcccgtta ttacgactgc tggtgggaat tggaaatcct gaatgcctcc gcattggaag
      661 attgggaaat atcgggaaag cctgaaaaat gggagaaatg gacagaaaaa tatcagaccg
      721 aagcgttaca gtcagttaag gatctatttg ccgaagaagt tcctgaaaat aatatagttt
      781 gcgatatttc tcattttttg cattttgccg atcgggcaga agccgacatt cccaatcaat
      841 cacgtttata tgccgaatat tggtgggaaa tggaaacgtt caaaatgtcg gttttaaaag
      901 attgggagcg ttcaggcaga ccactagatt gggaaaaatg gcaaaggcaa tatcaatccg
      961 aagcatttga attagtcaaa aatttgaacc gaagcaaaca agagtaacaa gccgcagcct
     1021 gcataggggt ttttaaagta cggcagcaac cgtagattag attaaaaatc aaacaatcaa
     1081 agcccgcctg atataatcga tatatcctgt taaacgaaaa ataaatatgt ttaaaaaatt
     1141 caaaccggta ttgttgtcat tttttgcact tgtatttgcc ttttggctgg gaacgggcat
     1201 tgcctatgag attaatccgc gttggttttt gagcgatacg gcaactgaaa tacctgaaaa
     1261 tccgaatact tttgtggcga aacttgcccg cctgttccga aatgccgaca gggcggttgt
     1321 catcgtgaag gaatcgatga ggacggagga aagccttgcc ggagctgtgg atgacggtcc
     1381 gttgcagtcg gagaaggatt atctcgcgct cgctgtccgg ctcagtcgtt tgaaagaaaa
     1441 ggcgaaatgg tttcacgtaa cggagcagga acatggggaa gaggtctggc tggattacta
     1501 tatcggcgag ggcggtttgg ttgcggtttc gcttttgcaa cgctcgccgg aagcgtttgt
     1561 taatgccgaa tatctgtatc ggaacgatcg tccgttttct gtaaatgtgt acggcggaac
     1621 ggctcacggg gaaaattatg aaacgacagg agaatatcgg gttgtttggc aaccggacgg
     1681 ttcggtattt gatgcgtcgg ggcgcgggaa aatcggggaa gatgtttatg agcattgcct
     1741 cgggtgttat cagatggccc aggtatattt ggcgaaatac cgggatgtcg cgaatgatga
     1801 gcagaaggtt tgggacttcc gcgaagagag caaccggatt gcatcggact cgcgcgattc
     1861 tgtgttttat cagaatatgc gggaattgat gccccggggg atgaaggcga acagtcttgt
     1921 ggtcggctat gatgcggacg gtctgccgca aaaagtctat tggagtttcg acaatggaaa
     1981 aaaacgccag agtttcgaat attatttgaa aaacggaaat ctttttattg cacaatcttc
     2041 gacggtagca ttgaaagcgg atggcgtaac ggcggatatg cagacctatc atgcgcaaca
     2101 gacgtggtat ttggatggcg ggcggattat ccgcgaagag aaacagggag acagactgcc
     2161 tgattttcct ttgaacttgg aagatttgga aaaagaggtg agccgttatg cagaggctgc
     2221 ggcgagacgt tcgggcggca ggcgcggcct ttctcactga acgcaaatac cgtctgaaac
     2281 cggttttcag acggcattgg agagtcaaac gctttttgca atgccgtata tgccgaaccc
     2341 ggttcgctac aaattgaaaa ccacaatcca aaaaacggaa atacacatta tcaaacaaac
     2401 aaataagttg acatattgtg cgatattgag ggctgtaccg tattttcccg ttctttatat
     2461 gagcggcagg catcctcata aaagaaggga taccgataag gaaaaatccc tcggtataaa
     2521 aaatatactg attaatcaat acgaatccaa caaacagttc atcattttct tttctgttgg
     2581 cgtataaaaa ccgctcggaa aaaaataaaa tatcaaaacg gcaaaataaa aatactgata
     2641 tatgccttca ctttcatagg gcgacgctct atgtgccgtc ctgtgtgttg aaacgtcggt
     2701 tttccttacc gcctgccccg cttttccctg attgctgcca actgccgtcc gaacctgaac
     2761 cgtcaggttt cagacggcat tttttctccc tgttcccaca attgccgttt ttgtgcggcg
     2821 gtcagttttt tgacggcgat ttcctgtttg agcgcagtgc ctttatccat gccgcctgca
     2881 acgatacgca tcgccaccgg tttgaatacg cgggtatatt tcgcgccttt tccggctgtg
     2941 tggatggcaa gccgctgttg cggattcggg ctgataccgc aatagaacgc gctgttttca
     3001 cacaatatca ggtaaacgct ccagtttgac gcgttcatct gcctgcatcc tgggtttttt
     3061 ccccgttcca aggcgcaact ttcagcagca gcagcatacc cggcagggca agtatgaaac
     3121 acagccggaa aaacggtaca taacccatcc attcgatcag atagcctgca aaggaattga
     3181 tgaccgtgcg cggcacggcg gacaggctgg taaacagcgc aagctgcgtt gccgtaaacg
     3241 cgggattggt ctcgcgcgcc atatacgata caaacgccgc cgtccccaac cccacgccga
     3301 ccgcttccgc gccgataact gccgccagca tcagcctctc gcctgtgccg accgtgtcga
     3361 aatgtccgaa ccctgccagc catacaaacc ccaaaaccgt tattgcctgc accgcgccga
     3421 atagccacaa ggctttgttt acgccgattt tcagcatcca cacgccgccc aagatacctg
     3481 ccgccactgc cggccacagt cctgcatttt tggcaatcaa accgatgtcg gtcttgctga
     3541 aacccatatc cagataaaac ggcgttgcca acgcggttgc catactgtcg ccgagtttgt
     3601 aaaggaagat aaacagcagc acgcacaccg ccgaagcgat gcccttgcgc gtaaaaaatt
     3661 ctttaaacgg ctctaccacg gtctgcttca acgttttagg aacggaaggc ggcaacacgg
     3721 gttcgtgcgc gagaaacagc gtcatcagaa ggccgggcag cataaataat gaagtgataa
     3781 caaatacttc tgaccacggc atcctgtctg ccaacaccaa actcaatgaa ccgggaatca
     3841 gggcggcaac ccggtaggcg ttcacatgaa ccgagttgcc caaacccaat tcttcgtccg
     3901 acaaaatctc gcgcctgaac gcatccaata caatgtcctg actggcggaa aaaaaagcga
     3961 caagcaccga caggccggca atcaacggca gatgattccg ggggtttaaa aaggcatatg
     4021 ccgccaaagc cgccagtaac cctgcctgcg tcagcagcat ccacccgcgc cgccgtccca
     4081 aaacgggcag cctgaccgcg tccatcagcg gcgaccacaa aaatttccaa gtaaacggca
     4141 gaccgatcaa cgccatcagc ccgatgctct tcaaatccac ctgctcgctg cgcaaccacg
     4201 ccggaatcag gttaatcaga aagtacagcg gcagccccga ggcaaacccc gtaaaaatac
     4261 agacgagcat attacgggaa aagatttgcc cgataaaacc tgatttcgat gcagtcatcg
     4321 ttcgtacagc caaaaaacaa aagcctattg tagcaaatat cgcccgcctg ctgaaaaatg
     4381 ccgccacgcg ccgccggttt gccgttccaa tgcaaaatgc cgtctgaaac tgtgttcaga
     4441 cggcattgct actgaaaccc gacggtttac aggctgtaat acatttcaaa ttccagcggg
     4501 tgcggcgcca tacggatgcg gcggacatct tcctctttaa aggcgatgta gctgtcgatc
     4561 cagtctttgc tgaacacgcc gccgcgcagc aggaattcgt ggtcggcctt gagggcggca
     4621 agtgcttctt ccaaagaagc gcagacggtc gggacgagcg cgtcttcttc cggcggcagg
     4681 tcgtacaggt ttttatcggc agggtcgccc ggatggattt tgttttgaat gccgtccaaa
     4741 ccggccatca gcagggcagc aaatgccaaa tacgggttgg cggtcgggtc ggggaaacgc
     4801 gcttcgatgc ggcgcgcctt gctgctgttc acagacggga tacggatgga agcggaacgg
     4861 tttttggcgg aataggccaa tttggtcggt gcttcaaagt gcggcacgag gcgtttgtag
     4921 gagttggtgg acggattggt aatcgcgttc agggctttgg cgtgtttgat gatgccgccg
     4981 atgtagtaga gcgcggtatc ggacaaaccg gcatagccgt cgcctgcgaa caggttttga
     5041 ccgtctttcc aaatggattg gtggacgtgc ataccgctgc cgttgtcgcc cataatcggt
     5101 ttgggcataa aggtggcggt tttgccgaaa ttgtgggcaa cgttttggat gacgtatttc
     5161 atatcttggg tttggtcggc gcgtttgacc aaagtggcga aacgggtgcc gatttccatt
     5221 tggctgccgg taccgacttc gctgtggtgg acttcgactt cgatgccgag tccttccaaa
     5281 atgttcacca tggcggagcg caaatcttga ccgcagtcaa tcggcgcgac gggcgcgtag
     5341 ccgcctttga cggcggggcg gtggccggtg ttttgaccgt ccatatgcag gccgcttgcc
     5401 cacgcgccgc tttcggacgt gatttcgtaa cgggttttgt gcatgtcggt ttcaaattct
     5461 acgccgtcga agacgaagaa ttcgggttcg gggccgaaat aggcggtgtc gccgataccg
     5521 gaagatttca aataggcttc ggcgcggcgt gcgatggagc gcgggtcgcg gtcgtaaccc
     5581 tgaccgtcgg cagggtcgat gacgtcgcag gtaatgacga cggtaacatc atcataaaaa
     5641 ggatcgacga aggctgtgga cgcatcggga cgcagctgca tatcggaagc ctcaatgcct
     5701 ttccagccgc cgatggacga gccgtcaaac gccggtccgt tttcaaacca ctcttcgggg
     5761 tcttccaaca cgatgcgcgc aggcacggta aagtggtgct gcttgccttt ggtatcggtg
     5821 aagcgcaaat cgacaaagcg ggcttcgctt tcttcaatca attttacggc gtttttaatg
     5881 gacatcttca gctcctgaaa aagggaaatg gcaaaatatg cggaacgcgg cggcttgacg
     5941 caaagtcggc acgattttac cacgccgatg ccgtctgaaa ccggttttgc gtcaaattcc
     6001 aagccggaaa atatagtttc aaacggcaag attgtcaaca atatgcttcg gtttgtctgt
     6061 tcaaaagcgg aatgcccgcc cttttgtccg aatgccgaca aaacagatgc cgtctgaagg
     6121 cggtttcaga cggcatcgtt cccatcttcg cgccgccgtt ataaaatgac cgccccgcct
     6181 ttatggaata tgactatgca cgccctcccc cgctacgccg ttttcggcaa ccccgccgcc
     6241 cacagcaaat cgccgcagat tcatcagcaa tttgcccttc aggaaggcgt tgacatcgaa
     6301 tacggacgca tttgcgccga catcggcggt ttcgcgcagg cggtttcgac attttttgaa
     6361 acaggcggtt gcggggcaaa cgttaccgta ccgttcaaac aggaagcgtt tcatctggcg
     6421 gacgaacatt ccgaccgcgc tttggctgcc ggtgcggtca atacgctggt gtggttggaa
     6481 gacggcagaa tacgcggcga caataccgac ggtatcggtt tggccaacga catcacgcag
     6541 gtcaaaaaca ttgccatcga gggcaaaacc atcttgcttt tgggcgcggg cggcgcggtg
     6601 cgcggcgtga ttccggtttt gaaagaacac cgccctgccc gtatcgtcat tgccaaccgt
     6661 acccgcgcca aagccgagga attggcgcgg cttttcggca ttgaagccgt cccgatggcg
     6721 gatgtgaacg gcggtttcga tatcatcatc aacggcacgt ccggcggctt gagcggtcag
     6781 cttcctgccg tcagccctaa aattttccgc gactgccgcc ttgcctacga tatggtttac
     6841 ggcgaagcgg caaaaccgtt tttagatttt gcacgacaat cgggtgcgaa aaaaactgcc
     6901 gacggactgg gtatgctagt cggtcaggcg gcggcttcct acgccctctg gcgcggtttc
     6961 aaaccggaca tccgccccgt catcgaacac atgaaagccc tgtaaccatg ttccgcatcg
     7021 tcaaatggct gattgccctg cccgtcggca tctttatctt cttcaatgcc tatgtgtacg
     7081 gcaacatcat tacctaccgc gccgtcgcgc cccatcggac tgcctttatg tcgatgcgga
     7141 tgaagcagtt tgaacaagaa ggtcgcgatg tcgcactgga ttaccgctgg atgccctaca
     7201 aacgcatttc caccaacctg aaaaaagccc tgattgcttc cgaagatgtc cgttttgccg
     7261 gacacggcgg cttcgattgg ggcggcattc aaaacgccat caggcgcaac cggaacagcg
     7321 gcgaagtgaa ggcgggcgga tcgaccatca gccagcagct tgccaaaaac ctcttcctca
     7381 acgaaagccg caactatctg cgcaaagggg aagaggcggc cattacggca atgatggaag
     7441 ctgttaccga caaaaacagg attttcgaac tgtatttaaa ctcaatcgaa tggcactacg
     7501 gcgttttcgg cgcggaagct gcgtcccggt atttttataa aaaaccggcc gcagacctga
     7561 ccaaacagca ggcggcgaaa ctgacggcac tcgtccccgc cccgctctac tacgctgacc
     7621 atccgaaaag caaacggctg cgcaacaaaa ccaatatcgt gctcagacgc atgggttcgg
     7681 cagaattacc cgaaagcgat acggactgat tgtttcagat atggaaatgc cgtctgaact
     7741 gcggttcgga cggcatatgt tttctggttc ttatagtgga ttaaatttaa accggtacgg
     7801 cgttaccccg ccttgccgta ctatgttgta ctgtctgcgg cttcgtcgct ttgtcctgat
     7861 ttttgttaat ccactaacct gcccgacagc cgcaaagacc atgtcgtgca tggcaggtat
     7921 ttgcggtgcg ggactgctgc cgaaaacggc aggcaaaaaa taaccgctta taagcggttt
     7981 tttggtggcc agaggcggaa tcgaaccgcc gacacacgga ttttcaatcc gttgctctac
     8041 caactgagct atctggcctg tatctgtctc gttaagaggt gcgtattaaa ccaaatttcg
     8101 ggacgacacg caagcggatt tgtcgatatt ttttacccaa ccgactaagt cgatgtttta
     8161 agtttaaatt aatttcacag aaaaatagtg caaatcggtt ttttgccgtt cgttcggcga
     8221 tgttctgctt tgcagacggc aaaacgcttc aatatttgaa gttctcgccc aaatagacgg
     8281 cgcgcacctg ttcgttgccg accaaatcgt cgggcttgcc ggaagccagc accgcaccgt
     8341 ctgaaatgat gtaggcgcgg tcgcagatgc tgagggtctc gcggacgttg tggtcggtaa
     8401 tcaatacgcc gatgccgcgc gatttgagga aaccgatgat tttctggatg tcgatgacgg
     8461 cgatgggatc gacgccggca aaaggttcgt ccaaaaggat gaaacgtggt ttcatggcga
     8521 ggacacgggc gatttcgacg cgccgccgtt cgccgccgga cagcgacggc gcaggattgc
     8581 ggcgcaagtg tccgatattg aggtcggcga gcagtttttc gatttctctg tcgatttgat
     8641 ttttatcttt ggttctgatt tccaagatgg cgcggatgtt ttgttcgacg gtcattttgc
     8701 ggaatatcga ggcttcctgc ggcaggtagc cgacacccag gcgggcgcgt tcgtgtatgg
     8761 gcaggtggcg caattcccgc ccgtccagca tcacgctgcc tgcgtcggca gcgatgagcc
     8821 cgactatcat gtagaagccg gtggttttgc ctgcgccgtt gggcccgagc agtccgatga
     8881 cttcgccgct ttcgatctca agggagaaac ttttgacgac ttggcgtttt ttgaaacttt
     8941 tctgcaggtt ttggacgacg aggcggctgt tgtttgcgct cataaatctc ttctcggcat
     9001 ttcggtcggc aaatgccgtc tgaactgcgt ttccgtttca gacggcatcg gggttattcg
     9061 gttttttgtg tgcttgaagg ctggatgacg acgctgaccc tgccggtttt ggaagcggat
     9121 ttcgcacccg atttcgtgct gccgttgatg gtatagactt cggttttggt gttgtaggta
     9181 atgaccgcac cttcggcaac gtcgccgccg cgctgcactt tggcattgcc ggtcagaacg
     9241 acggtgcttc ctgcggagga ataggtaacg ttgtttgcct gaccgcgcac cgtccctttg
     9301 cccccgtcca acgtttggct gaagcggacg ggcgaacctt ccgccctcac ggattcgccg
     9361 cctttgccgc cgcgtgtgac gttgacgcgc gaggcggaaa tgttgagcgt accctgtctg
     9421 atgatgacat tgccgctaaa tgtggtactt tggttggctt gatcgagcga accttggtcg
     9481 gcttcgattt ggatgggccg tctgctgtcg ctttgaaggg caaaagcggg agaagttgca
     9541 aaaattacaa ttaaaacaaa tagcttacat atcttttgta tcataaatcg cggctttcac
     9601 tttagatgag aagttcaaca tgcctgtttt gtggttgtag gtcataccgc ccgcctgacc
     9661 gtgcgacgcg ccatattgga aactgacagg cgtatcggtt tgggcatatt gagattcggt
     9721 atcgacgtgc agtttttcgg tttcgacttt acccgcctgc cgcctgccgt cggcggtttt
     9781 ggtcagcaca acgttgtttt taaaaagaac ctgtttgttt tcggtatggt aaacggcttc
     9841 atcgctgccg acttcgtaca acagcctgcc ttcttggaag aacacgagat gcggcgaatc
     9901 aaaatggatg tcgctgtttt cgggaaactg tttcgcacct ttcgcgctca aatgttcttt
     9961 caagtatccc tgttcgtcaa accgccttcc gtccaagccg tccattgtgt attgaggttc
    10021 gtcgggattg agcctgactt cctcgatttc gacttcgctg atacggccca accatgccga
    10081 caggccgccc aaggcaaccg ccaatatcaa tgggaacgca attccgtacc gccatcttac
    10141 tttcatttga tgtacccgtt caaagccgcg cccaacgtac ctttcgcgcg gataatcagg
    10201 tcacacactt cgcgcaccgc gcccgcaccg ccggggcgtt tggcaatata ggcggcgtgt
    10261 tgcagcgtaa accaatgcgc gtcggggacg gcaaccggca atccgcagcg caccatcacc
    10321 ggcaaatcga ccacgtcgtc gccgacaaag gcgcactcgg cttcttccaa gcctgcctgc
    10381 gcgcgcagct cttcatacgc ggcgcgtttg tccgaaatgc ctttgaagta gtaatttatg
    10441 cccaactgtt tgacgcggat gccgacggag ggcgcgtccc gacctgtgat aatcgcagtc
    10501 tgcacgccgc ttgcctgaag cattttcagg ccgtgtccgt ccagtgtgtg aaacgatttg
    10561 atttcttcgc cgtcgtcgcg gataaagatg cgcccgtcgg tcaaaacgcc gtccacatcc
    10621 aatatcagca gtttgatttt ggcggcgcgt gcctgtaatt cgggagaaat tgcctgcatt
    10681 gggatttcct tgttttcaga cggcatatct taccacaaat gccgtctgaa acggctgcaa
    10741 cgccttatac aatccgcgcc gccaaaaggt cgtgcatatt cagcgcacct gtcagcacgc
    10801 cgtctgcatc ggtaaccaga agcccgttca catggtttgc ctgcatgact ttcagggctt
    10861 cggtggcgag gcgttcggcg gagatggttt tagggtgtgt atgcatgact tcgtctatcg
    10921 aaagaccggt aaaattgtcg cattcttgaa acaggcggcg caaatcgccg tcagtaaaca
    10981 cgcctttcag acagccttgc ccgtccgtta ccgccaacat gcccaaacct ttctcgctca
    11041 tgctgacgat ggctcctttc aagggcgtgc cgagtcggac ggcgggcagg ccgccgcctt
    11101 tgtgcataat gtcggcaacg cgcaaaagca ggcgtttgcc gaggctgccg gcagggtgga
    11161 tcaaggcgaa gtcgtcgggc gtgaacgcgc gggcgcgcag caggacgacc gccaacgcgt
    11221 cgcccaaagc cataacggcg gtggtgctgg tggtcggggc aagccccaac gggcaggctt
    11281 cttgcgaaac cgatgcggtg atgtggatat cggcatggcg cgccatggtt gaatcggggc
    11341 gggcggtgat gcagacgagg gtaatgtctt tgcgtttgag cgcggggatg atggcggtga
    11401 tttcgtcgct ttcgccggaa ttggaaatgg cggcgacgac gtcgttgtcc acaatcatgc
    11461 ccaaatcgcc gtgtgccgct tccgcagggt ggacgaaaaa cgcgggcgtg ccggtcgagg
    11521 ccatggttgc cgccattttg cgcccgatat gtccggactt gcccataccc gtgatgacga
    11581 ccctgccctt gcagtgcaac aacgcgtctg ccgcaaggac gaagttttcg tccaattccg
    11641 ccgcaatttc gcgcaagcct tccgcttcgg tgtgcaacac ttcgcgcgcc cagtcgagat
    11701 atttttcgtt ttctgccatt gccatccgcc tttgctgtaa caaaactacc caaaatttcc
    11761 gaatcggggt agcataagcc ttcaaacccc gcccatccat ccaaaggaat caaaaatgac
    11821 tattttatcg gacgttaaag cattaggaca acaaatctgg ctggacaacc tttcccgctc
    11881 gctcgtgcaa agcggcgaat tggcgcaaat gctcaaacaa ggcgtgtgcg gcgtaacttc
    11941 caatcccgcc attttccaaa aagccttcgc cggcgacgcg ctttatgccg acgaggtcgc
    12001 cgccctcaag cggcaaaacc tcagccccaa acaacgctac gaaaccatgg cggttgccga
    12061 cgtacgggca gcctgcgacg tttgccttgc cgaacacgaa tccaccggcg gcaaaaccgg
    12121 tttcgtcagt ctcgaagttt ctcccgaact tgccaaagac gctcaaggca cggtagaaga
    12181 agcccgccgc ctccatgccg ccatcgcgcg taaaaacgcc atgattaaag tgcctgccac
    12241 cgacgcaggc atcgatgcgc tcgaaaccct cgtttcagac ggcatcagcg tgaacctgac
    12301 cctgctgttc tcacgcgccc aaaccctcaa agcctacgcc gcctacgcgc gcggcattgc
    12361 caaacgcttg gcagccggac aaagcgttgc ccatatccaa gttgtcgcca gcttcttcat
    12421 ctcgcgcgtg gacagtgcgc tggatgcaac gctgcccgac cggctcaaag gcaaaaccgc
    12481 catcgccctt gccaaagccg cctatcaaga ttgggaacaa tatttcaccg cccccgaatt
    12541 tgccgcactg gaagcccaag gcgcaaaccg cgtgcagctt ttatgggcat ctaccggcgt
    12601 gaaaaaccca gcctatcccg acacgctcta cgttgacagc ctgatcggcg tgcacaccgt
    12661 caacaccgtc cccgatgcca cgctcaaagc ctttatcgac cacggcacgg cgaaagcgac
    12721 gctgaccgaa agcgcggacg aagcacgggc gcggctcgcc gaaattgccg cgctcggcat
    12781 cgatgtcgaa accttggcgg cgcgtttgca ggaagacggt ttgaaacagt ttgaagaagc
    12841 ctttgaaaaa ctgctcgcgc ctttggttta ggcatttccc cgatgccgtc tgaaaggctt
    12901 tcagacggca tcgccctttt gaccggacac agtgcggcat atcaaggatc cgccctaaaa
    12961 atattaatgg gcaagaagtt aatgcacgac attacccgcc aacacaaaag gaaatagaat
    13021 catggcaaaa gcactcgaaa tcatttcacc cgatgaaatt tattcagatc tgatttttaa
    13081 ggatccggta cctccccata ctatatatac agaattgatg aaactggttg gtagaaatgc
    13141 aggcaatgag agaattgcag atagagcctt cgattttttc tcgcctgctc ttatggatga
    13201 ttcagctacc gaagagcaat ataatgccct atatgattta accttgcttg aagagcccgg
    13261 gatggaactg gataaagatg agataacggc tttgattaat agtcttaaat aaaggggctg
    13321 taccggatat gtacacagga cgcttcgccc ccagcccgac cgggttgctc cacatcggct
    13381 ctcttctgac cgccgtcgct tcctatgccg atgcacgttc aaacggcggc aaatggctgg
    13441 tccgtatgga agacctcgat ccgccgcgcg aaatgccggg ggcggcaagc catatcctgc
    13501 acacgctcga ggcattcgga ttcgagtggg acggcgaagt cacctatcag agccgccgtt
    13561 acgccctgta tgaagaaacc ctataccgtc tgaaaaccgc cggactggtc tatccctgcc
    13621 attgcaaccg caaagactgg caggccgggg caaggcgggg cacagacggg ttcgtctata
    13681 acggacgctg ccgccacccc ggccaacgcc ctgcactgca aggcaaacag ccgtcgtggc
    13741 gcatccgcgt ccccgaccgc gacatcggct tttcagacgg catcgtcggc agttacaccc
    13801 aaaacctcgc ccgcgacatc ggcgatttcg tcctgttccg tgcagacggt tactgggcat
    13861 accagctcgc cgtcgttgcc gacgatgccg aacagggcgt tacccacatc gtgcgcggac
    13921 aagacctgct cgtttccaca ccgcgccaaa tctatttgca gcagtgtttg ggcgttccga
    13981 caccgcaata tgcccacctg ccgctgctga ccaacgcgca agggcagaaa tggtcgaagc
    14041 agacgctcgc ccccgcattg gattcaaacc gccgcgaaca acttctccgc caagtgttcc
    14101 gctacctcaa gctgcccgaa gcaccggaaa ccgaccgccc tgccgaattg ctcgactggg
    14161 cggtggcaca ctgggatatg gacaaagtgc cgaaacacgc cattaccgcc ccctaaacac
    14221 cacccgacaa acgcacaaat gccgtctgaa accttcagac ggcatttcac acatacagcc
    14281 ccgccctatt cgcgcgtatt tgcccgttcg acttctttcc acgcttcgaa aatcaggccg
    14341 ccgtcgttgt ctttcaagag cgttgcatcg gaaagcatac gccgccaagt ccgcgcgcct
    14401 ttcaaaccgt gcatcagccc gaggctgtgg cggacgatgt ggcgcaggat tgtgccgcgt
    14461 ccggcttgga tttggacttg gctgtatgtg tagaggcgct gcaccaaatc ggcgtattcg
    14521 atcggggcgc ggttgtcacc gtaaaacagc ctgtcccatt cgcgcatcac catcgggttg
    14581 tggtatgcct cgcgcccgac catcacgccg tcaacgtgtt gcaagtgtcc ggcgattgct
    14641 tcgttggtgg tgatgccgcc gttgatgatg atttccagct cgggaaactc ctgcttgagg
    14701 cggtaaacgt aatcgtattt caagggcgga acatcgcggt tttctttggg cgaaagaccg
    14761 tccagccaag cgttgcgggc gtggacgatg aaggttttgc aggcggtttt gtcgcgcagc
    14821 gtgccgacga aatcggcaac ggtttggtat tcggtctgcc tgtccacacc gatgcggtgt
    14881 ttgacggtaa cggggatgcc gaccgcgtcg tgcatggcgt tgaggcagtc ggcaaccagc
    14941 ccgacttcgt tcatcagaca cgcgccgaac gcgcctttct gcacgcgcgg actgggacag
    15001 ccgcagttga ggttgacctc gttgtagccg tattcttcgg cggctttggc ggctttcgcc
    15061 aaatcggacg gatcgctgcc gcccagttgc agggcgacgg gctgctcgcc ttcgttaaac
    15121 atcaaaaaac ggtctttgtc gccgtaaaca atcgcgccgg cgttgaccat ttcgctgtaa
    15181 agccaagcat ttcgggtaat ctggcgggca aggtaacggt agtgcctgtc cgtccagtcg
    15241 agcatcgggg cgacgcataa acgccttttc cctttatgtt caataagttc cttgtttatc
    15301 ataaggtttt cacattcttt taactcaaac attctttatt atttgcttgg atttttgccc
    15361 atttcactat atttgacgta ccatacttgc accataattt ttaattcttg gaatggtgca
    15421 aatggcaaca ataaccaagc ggcgcaatcc ttccggcgaa acagtatatc gggttcaggt
    15481 acgggtcggc aagaaaggct atcctgcttt caatgagagc aggacgttca gtaaaaaggc
    15541 tttggcggtc gaatggggga agaaacggga ggcggaaatc gaagccggcc cagaactgct
    15601 tttcaagcgc ggcaaggtca agatgatgac gctgtccgaa gctatgcgga agtatcttaa
    15661 cgaaacgctt ggagcgggtc ggtcaaagaa aatgggcttg cgttttctga tggagttccc
    15721 gattggcggc atcggcatcg ataagctgaa acggtctgat ttcgcggaac acgttatgca
    15781 gaggcggcgc ggaatccccg aactggacat tgcgccaatc gcggcttcta cggcattgca
    15841 ggagctgcaa tatatccgtt ccgtgctaaa acacgccttt tatgtgtggg ggcttgaaat
    15901 aggctggcag gaattggatt tcgcggcaaa cgggctgaaa cgctcgaata tggttgcgaa
    15961 atctgcaatc agggacagat tgccgaccac ggaagaactg caaaccctga caacttattt
    16021 cctgcggcaa tggcaaagca ggaaatcttc cataccgatg cacctgatta tgtggctggc
    16081 gatttatacg tcaaggcggc aggatgagat ttgccgcctg ctgtttgacg actggcacaa
    16141 aaatgattgt acccggccgg ttcgtgattt aaaaaatccg aacggcagca cagggaataa
    16201 taaggagttt gatattctgc ctatggcttt gccggtcatt gacgagctgc cggaggaatc
    16261 ggtcaggaag cgtatgcttg ccaacaaagg catcgccgac agccttgtac cgtgcaacgg
    16321 aaaatcggtt tccgccgctt ggacgagggc gtgcaaggtt cttagaatta aagacttgcg
    16381 cttccacgat ttacggcacg aggctgctac ccgtatggct gaagacggct tcacgattcc
    16441 gcaaatgcaa cgggtaacgc tgcatgacgg ttggaacagc ctgcagaggt atgtgagcgt
    16501 acgcaaacgc tcgacgcggc tggattttaa agaggcaatg atgcaggcgc aaagcgatat
    16561 aaaatccggg aagtgatgtt aaattaaagg ggatgtgccg catcccctga ttcctccgtc
    16621 ttgcatcaag accggaattt ccgatattcc gattttgcct tttcttcctt gtctgcgatt
    16681 gcctttgcaa gctctcggac gctgacgaaa tattccgatt ttcggctgtc ggacaatcgg
    16741 aaaatcggaa acggaagctc gcaccgtacc cccgatgctt taaacgcgtt aaagccgata
    16801 tgggcaaaaa agtctttgtg tacttccctc ggcgggatat acgggcggcc gccgtatgcg
    16861 attaaaagcc cttgttcttt actgatgttc atatatacct ctttctgtta attctgcttt
    16921 gtccgggttt ccgctttttt acggcttggc ggatgttcag cctgcgtcgg tgcataagtc
    16981 ctcctgttcg ggcggttgtt cagccggccg cccctccgaa aagtggcagg ctgcacggga
    17041 agtagtgttt ttttccgtat ttctctgcct gtatcaggtt gcacaggcgg tcgcaaaagc
    17101 cgtcaaattc ggcatgcggc cagccttcta tctcgttgac cattttgctg aacctcaatt
    17161 cccgggcaat ccgctttgcc gctgcgtcac ggcattcgtg ttccaagagg acggggtctt
    17221 ccttttcctt cggcggtgca ggttcttgca cggtttgcca caagtccggt tcgatttgcc
    17281 aagtgtcggc ggcaagcagg gtgtctgttt tgcagtcgta cagttcgcgg atgcagccgc
    17341 cgtcggtttt gtctttgtcg ataaccagta aaagcacttc gatcggcgtg tcttcaaacg
    17401 cgttttgtat gcggttgagt tctgccagcc ggttgccgat gatttcgcgg aatcgctgct
    17461 cgcttttgcg gtaggcgatg ccggggaaca ggatgaagaa tccgaagcgg cgggcgtttt
    17521 ccaatccttt taaaacgaag atttcatcgg ctacgcccga tttcttccac gggtattcct
    17581 gtgcgatacg gcttttttcg tcttcgctca aatcctttag cttgatggaa aacggcggat
    17641 tcatgacggt gcagtcttgc ggttcgccgt cggtataaag gaaaaggctt gtgttgtata
    17701 ctttggcggc agggtagttt tgcaacaagg ctttacacgc ttccgcctgt atctctacgg
    17761 cgcggaaatc ggacggttcg atgtactgct ccagctgccc gcttcctgcc gcgccgtcaa
    17821 atacgcccgg gtgttcgccg cagtatcggc ggactttggc ggcaaccagc cgcctcaagc
    17881 tgccgcccgt gatgtattcg gcgtagccgc cggctttttt acggttgttg tgtccttgaa
    17941 acgtcatggg ttttcgtccg ttctctggct tgtccgggtg tggcggagcg tttgcggatg
    18001 cggctgacgc gctgcggttt gccgtcttcg ccgcgctgaa aaacgcttgt tccgccgcgc
    18061 cggcagccgt gccgcttatg cctgatgccg tgttt
//
LOCUS       DBJGGI010000039        17831 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.039, whole genome shotgun sequence.
ACCESSION   DBJGGI010000039 DBJGGI010000000
VERSION     DBJGGI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17831
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.039"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(<1..146)
                     /locus_tag="ACSIGJ_001329"
     CDS             complement(<1..146)
                     /locus_tag="ACSIGJ_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HHQ1330179.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEM"
     gene            complement(265..1365)
                     /locus_tag="ACSIGJ_001330"
     CDS             complement(265..1365)
                     /locus_tag="ACSIGJ_001330"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HHQ1330180.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRKLLDLQDTGTDELHRSRMLSQELSRLYAQTAAELLCSQNLAPCDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAELTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRE
                     TRVVLNIGGIANISVLPPGAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLGRLLAHPYFSQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVC
                     DAVSHAAADARQMYICGGGIRNPVLMADLAECFGTRVSLHSTAELNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            1690..3264
                     /locus_tag="ACSIGJ_001331"
     CDS             1690..3264
                     /locus_tag="ACSIGJ_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HHQ1330181.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTD
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASRKGLPVSTTHAIIGGIVGSA
                     LCMAFTHNADGVALIRWGKLGEIGMSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGTMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQSAVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSMASYLILQAVF"
     gene            3278..3466
                     /locus_tag="ACSIGJ_001332"
     CDS             3278..3466
                     /locus_tag="ACSIGJ_001332"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330182.1"
                     /translation="MPSEPVFRRHFVDGMCGLVPYAQSVLSGYAVWYLGFGTVGHKGY
                     DMPPPVLENTYPRQQNAV"
     gene            complement(3504..4925)
                     /gene="hemN"
                     /locus_tag="ACSIGJ_001333"
     CDS             complement(3504..4925)
                     /gene="hemN"
                     /locus_tag="ACSIGJ_001333"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HHQ1330183.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKVLHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADTYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPSGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSVGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            5060..5794
                     /gene="fnr"
                     /locus_tag="ACSIGJ_001334"
     CDS             5060..5794
                     /gene="fnr"
                     /locus_tag="ACSIGJ_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HHQ1330184.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNEFSQLDAVIRQSR
                     RLKKGEYLFRAGGAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSYV
                     HSCDAVALENSEVCELPFTHIEELGQNIPSLRTHFFRMISREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            complement(5907..6857)
                     /locus_tag="ACSIGJ_001335"
     CDS             complement(5907..6857)
                     /locus_tag="ACSIGJ_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HHQ1330185.1"
                     /translation="MKLQQLKYALEVYRHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIAQMVTSGESDLAIVTERIDDHPELGR
                     LSCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFAKARLE
                     RPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            7221..7931
                     /gene="rmpM"
                     /locus_tag="ACSIGJ_001336"
     CDS             7221..7931
                     /gene="rmpM"
                     /locus_tag="ACSIGJ_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HHQ1330186.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAVPEPEPAPVAVVEQAPQYVDETISLSAKTLFGFD
                     KDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSEKYNQALSERRAYVVANNLV
                     SNGVPASRISAVGLGESQAQMTQVCQAEVAKLGAKASKAKKREALIACIEPDRRVDVK
                     IRSIVTRQVVPARNHHQH"
     gene            8706..9662
                     /gene="thiL"
                     /locus_tag="ACSIGJ_001337"
     CDS             8706..9662
                     /gene="thiL"
                     /locus_tag="ACSIGJ_001337"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HHQ1330187.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEGLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTCIGKINGGCRLKVLDAGGRELELHSLGFDHFG"
     gene            9655..10140
                     /locus_tag="ACSIGJ_001338"
     CDS             9655..10140
                     /locus_tag="ACSIGJ_001338"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A family protein"
                     /protein_id="HHQ1330188.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGAFGTLAALPLA
                     FVLILLGIDGLLLAFVCIVLFMWGIRICACAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPPPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(10200..11876)
                     /gene="ettA"
                     /locus_tag="ACSIGJ_001339"
     CDS             complement(10200..11876)
                     /gene="ettA"
                     /locus_tag="ACSIGJ_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HHQ1330189.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGT
                     GKSTVLRIMAGVDKEFEGEAVPMGDIKIGYLPQEPELDPEKTVREEVESGFGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPDWD
                     AKIGNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKPKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIARQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
     gene            12112..13466
                     /locus_tag="ACSIGJ_001340"
                     /pseudo
     CDS             12112..13466
                     /locus_tag="ACSIGJ_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
     gene            13470..14431
                     /locus_tag="ACSIGJ_001341"
                     /pseudo
     CDS             13470..14431
                     /locus_tag="ACSIGJ_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            14678..16935
                     /locus_tag="ACSIGJ_001342"
                     /pseudo
     CDS             14678..16935
                     /locus_tag="ACSIGJ_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
     gene            16948..17590
                     /gene="pgmB"
                     /locus_tag="ACSIGJ_001343"
                     /pseudo
     CDS             16948..17590
                     /gene="pgmB"
                     /locus_tag="ACSIGJ_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
     gene            17730..>17831
                     /locus_tag="ACSIGJ_001344"
     CDS             17730..>17831
                     /locus_tag="ACSIGJ_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HHQ1330190.1"
                     /translation="MSTFFRQTAQAMTAKHIGRFPLSELDQVIDWQPI"
BASE COUNT         3859 a         4644 c         4970 g         4358 t
ORIGIN      
        1 tccatttctt tgacggcggc gcgggacgat gcggcgacga gttcctgttc gacggcaaag
       61 cgtttgccgc cgccgtgggc gggtatgccg gtcagtgtgc cgcaggcggt gagtacgagg
      121 gggatgagga ggagcagggt tttcatggca gggtttgttt tgttttgaac gggtttgagt
      181 gtaaatggat tttaagggtt tgtaaacgaa aggggtggaa atgccgtctg aacggcggaa
      241 atggctttca gacggtattt gcgctcaata ataatatccc gcgcccagaa tacacggttt
      301 ggatgcgccg gtcgctttgt gcggactacc gggaatgcgg ttaatccaac acgccgccaa
      361 ccacgcaaat gcggccgcct ccacccattg aggatcgagg ttcagttcgg cggtgctgtg
      421 cagggaaacg cgtgtgccga aacattctgc caaatccgcc attaaaacag gattgcggat
      481 gccgccgccg caaatgtaca tttgacgggc atctgccgct gcgtgtgaga cggcgtcgca
      541 aacggtttgc gcggtgaatc gggaaagcgt ccgcaatacg tcgtatcggt tttcgccgcc
      601 gtcaaggtag gtttcgagcc aatttagggc aaacagttcg cgccccgtgc tttttgggtg
      661 gggttgtgag aaatacgggt gggcgagcag cctgccgagc agttgcggca atatgttgcc
      721 ttgtgccgcc tttgcaccgt ttttgtcgta aggcagctgc cagtgtgcct gcgtccacgc
      781 gtccatcagc atattgcccg gccctgtgtc gaagccgaag gcgggtgcgc cgggggggag
      841 tacgctgatg ttggcaatcc cgccgatgtt cagtaccacg cgtgtttccc tgtcatcgcg
      901 gaacagggct tcgtgaaagg cggggacgag cggcgcacct tgtccgccgg cagcaaggtc
      961 gcggctgcgg aagtcgccga cggtaaaaat ccgcgtcagt tccgccagca gcggcaaatc
     1021 ggcaagctgt atgctgtaac cgtgttccgg cgcgtgtcgg acggtttgcc cgtggcagcc
     1081 gagggcggta atgtcgcacg gagcgaggtt ttgactgcac agcagttcgg cggcggtttg
     1141 cgcgtacagg cggctgagtt cttgcgacaa catcctgctg cggtgcagtt cgtctgtgcc
     1201 tgtgtcctgc aaatccagca atttgcggcg caaccggtca gggtaggggg taaaggcgtg
     1261 cccttccgcg cccagccatt tgccgccgtc catccgtacc agcacggcat ccgccccgtc
     1321 catactggtt cccgacataa tgccgatgta aagctgtgtt tccatcatca ctcccaaact
     1381 ggtgcaaaac gccattttaa cgtgtattga cgctcgtata ccgatttgcc gccgcagtgt
     1441 aaataaagtg taaataaatg ttttaagacg ggtggaaaaa tattataatg cgcccgcaac
     1501 atccagtagt agaagtgtca tacaaaccgt ttccggcagc agttttgcat tcggtcaggt
     1561 ttgggggtat tcggatgcgg ttcagaagga aggatgcgtc tgccatatcc cgaaacggca
     1621 gttcgaccgg aggcagcaat acagtgtcgg caacactcat gatttccacc acattaaagg
     1681 aagattgcca tggctcaaat ccaaatgagc gcaaatgtta aaaccatcaa cgccgtcttt
     1741 gccgccatgc tggtaggtac agtcggctat tttatttatt ggggcttggg ttatacccat
     1801 tacaattacg ccgccttatt cattattgcc acgatgttcg gtgtgtttat ggcgttcaac
     1861 atcggcggca acgatgttgc caattctttc ggcaccagcg tcggtgcagg cacgctgacc
     1921 atcccgcagg ctttgctgat tgcggcggta tttgaggtca gcggcgcggt catcgcgggc
     1981 ggcgaggtaa ccgataccat acgcaaaggc atcgtcgatt tgaagggtgt tgatttcgaa
     2041 cccatacagt ttgtgtttat tatgatgtcc gcgcttttgg cggcggcgtt gtggctgctg
     2101 ttcgcgtccc gcaaaggcct gccggtttcg acgacgcacg cgattatcgg cggcatcgtc
     2161 ggcagtgcgt tgtgtatggc gtttacgcat aatgcggacg gggtggcgtt gatacggtgg
     2221 ggcaagctgg gcgaaatcgg gatgtcttgg gtattgtcgc ccgtgttggg cggcgcggtg
     2281 tcctatttcc tgttttcgcg cgtcaagaaa aacgtcttag attacaacgc ttgggcggaa
     2341 ggaacgctca agggcatcaa gcaggaaaaa aaggcctata aagaacggca ccgcctgttt
     2401 ttcgagggtt tgtccgaagc cgaaaaagtc gagtacgcca ccaaaatggc gcacgacgcg
     2461 caaatttacg acgaacccga attcgatcct caagagctgc aatcggagta ttaccgcggt
     2521 ctttatgcgt tcgacaaccg taaaaacaat gtcgattcct acaaggcgct gcattcttgg
     2581 attcccttta tcgcttcgtt cggcacgatg atgatttccg ctatgctgat tttcaagggc
     2641 ttgaaaaacc tgcatttggg gatgagcaac gtcaacagct tcctgaccat ctttatgata
     2701 ggcgcggcgg tgtggatggg gacgtttgtg tttgccaaaa gcctcaagcg taaagacttg
     2761 ggcaaatcga cctttcagat gttttcatgg atgcaggtct ttaccgcctg cggcttcgca
     2821 ttcagccacg gtgcgaacga tatcgccaac gccatcggcc cgtttgccgc gattatggat
     2881 gttttgcgga cgaacagcgt tgccgcgcaa agcgccgtcc ctccgattgc gatgctgact
     2941 ttcggcatcg cgctgattgt cggtttgtgg tttgtcggta aagaggtgat taaaaccgtc
     3001 ggtacgagtt tggcggaaat gcatcctgct tcgggtttta ccgccgaact gtccgccgca
     3061 tccgtcgtga tgggcgcgtc gctgatgggg ctgcccgtgt ccagtacgca tatcttggtc
     3121 ggcgcggtac tcggtatcgg tctggtcaac cgcaatgcca actggaaact gatgaaaccc
     3181 atcggtttgg cgtgggtcat taccctgcct gccgccgctg tattgtcgat ggccagctat
     3241 ttgattttgc aggcagtgtt ctgattgtaa aatactgatg ccgtctgaac ccgtgttcag
     3301 acggcatttt gttgatggaa tgtgcgggct tgtgccttat gcacaatctg ttctgtcggg
     3361 atatgctgtt tggtatcttg gtttcggaac ggtcggacac aaaggttatg atatgccgcc
     3421 gcctgttctt gaaaacactt atcctcggca gcaaaatgcc gtctgaaaaa gcctttcaga
     3481 cggcttttgt acgttagccg caatcacacc gtttgcgaat atttcgcttt ggtttctttg
     3541 tggcgcaggt ggtagtcgaa gaccatggcg atgttgcgga tgaggaagcg tcctttcggt
     3601 gtaacggtca gcccgtggct gttcaggcgc accaatccca aaccggcgag tttttccaaa
     3661 tccgccagtt cgtctttgaa gtagcggtcg aacgggatgc cgaacatact ttcgtaaatc
     3721 ctgtagtcga gcgcgaagcg gcacattaaa tcctgaatga tgttgcggcg caggatgtcg
     3781 tcctgattga gctggtagcc gcgcatgatg ggcagtctgc cttcgtcgat ggcggcatag
     3841 taggcatcga tgtcgcgttc gttttgggaa taggtgctgc cgattttgcc gatggacgac
     3901 acgccgatgg cgaccaaatc gcaatccgcg taggtcgaat agccttggaa gttgcgttgg
     3961 aggaagcctt ctttgagggc gatggagagt tcgtcgtcag gtttggcgaa atggtccatg
     4021 ccgatgaaga cgtagccgcg ctctgtcagg gtttggacgc agtattgcag catatcgagc
     4081 ttctcttcgc tgtcggggac ggcggcggta tcgatgcggc gttgcggttt gaacacgtgc
     4141 ggcaggtggg cgtagtggta aagggcgagg cggtcgggat cgagcgacaa aacggtatct
     4201 atggtggttt tgatgctttc ggaagtctga tgcggcaagc cgtaaatcaa atcgacgctg
     4261 acggatttga accctgcctc acgggcggca tcgatgactt ctttggtttc ttcgtagctt
     4321 tggatgcggt tgactgccgc ctgcactttg gggtcgaaat cctgaatgcc gacgctcatg
     4381 cggttgaagc cgagtctgcc gagcatgagg acggtgtcgc ggctgacttt gcgcgggtcg
     4441 atttcgatgg aatattcgcc ggacggtatc agttcgaaat gtttgcggat catgcggaag
     4501 acacgttcga tctgttcgtc gctcaaaaag gtcggtgtgc cgccgccgaa gtgcagttgg
     4561 gcaagctggt gccgtccgtt cagatgtgga gcgagcagtt ccatttcttt ttcaagatat
     4621 tcgatgtagg tatcggcgcg gcttttgtct ttggtgatga ttttgttgca gccgcagtag
     4681 tagcagatgg tgttgcaaaa cggaatgtga atgtaaaggg aaagcggttt gtttaacgcg
     4741 cccataccgc gcaaatgtaa aactttgata tattcgcctt cgcggaaacc gtcatggaaa
     4801 cggtcggcgg tagggtagga agtatagcgc ggaccgctgg cgggcaggct ggcaatcagc
     4861 gcgcggtcaa actcggggcg gtcatcgttt acattgtgat tgttctgtat ctgaatgatt
     4921 ttcatggtgt gtgtgcggtt ttatgatgtt agtcaaattt tggatagttt ggtagaatgc
     4981 cacagtatga taaacctgtc ttgatatgtg tcaacaagca cataatcggg atggggcaaa
     5041 acacacgaaa aggtacggta tggcttcgca taatactaca catcagatga aaacgctgtg
     5101 ttcttcctgt tctttgcggg aactctgcct gcctgtcggg ctgctgccca acgagttcag
     5161 ccaactcgat gccgtcatcc gccaaagccg ccgcctgaaa aaaggcgaat acctgttccg
     5221 tgccggcgga gcctttacct cgctctttgc catccgttcg ggcttcttca aaacaaccgt
     5281 cgccagtcag gacggccgcg atcaggtaac gggtttcttt atgtcgggcg aactcatcgg
     5341 catggacggc atctgttcct atgtgcacag ttgcgacgcg gtcgccttgg aaaacagcga
     5401 ggtatgcgag ttgccgttta cccatatcga agagctcggt cagaatatcc ccagcctgcg
     5461 tacacacttt ttccgcatga taagccgcga aattgtgcgc gatcaaggtg ttatgctgtt
     5521 gttgggcaat atgcgcgccg aagagcggat tgccgccttc ctgttgaacc tttcccaacg
     5581 cctttattcc cgaggtttcg ccgccaacga cttcatctta agaatgtccc gcgaagaaat
     5641 cggcagttat ctcgggctga aacttgaaac cgtcagccgc acattatcca agttccatca
     5701 ggaaggattg atttccgtcg aacacaaaca catcaagatt ctcaatctgc aggtgctgaa
     5761 aaaaatggta tccggttgtt cgcacgccat ttgattaacc tgtacgaaca tttcagacgg
     5821 cattctcaat aaacacaggg cagacgaaaa catctgccct gtttgttgta tctgccgcaa
     5881 agtgccgcct gaaaaccggc agccgcctaa atcgaaaaat cctcgctgat gggcgtgtac
     5941 agaatcctat ccaccttctc gcgtgtcagg tgcggcgcaa acgcttggat aaagtcgtag
     6001 gcatatccgc gcaaataagt atcgctgcgc aaagctatcc acgtcggcga cggctcgaac
     6061 aggtgtgccg catccacaag ctgcaaatcg ccgtccgtat ccgggttgta tgccattttc
     6121 gccatcagtc ccacgcccaa acccaagcgc acataagtct tcaatacatc cgtatccgcc
     6181 gccgccaatg cgacatccgg ccgttccaaa cgggctttgg cgaatgcccg cgcgatgctg
     6241 ctgcccgcat tgaatgcaaa ttcataagta atcagcggaa acctcgccaa atcttcaata
     6301 cggaggggat tcctgcattc gagcagcgga tggtcgttcg gcacaatcac cgcgtgtgtc
     6361 cagtcgtagc aggaaagcct tcccaactcg gggtggtcgt ctatccgttc cgtaacaatc
     6421 gccaaatcag attctcccga agtaaccatt tgggcgatgg cggcagggct tccctgtttg
     6481 atggtcagat tgactttcgg atagcgtttc acaaaatcgg caacaatcaa cggtagggca
     6541 tagcgcgcct gcgtatgcgt cgtggcaacc gtcagcgaac cgctgtcctg tccggtaaac
     6601 tcgctgccga tatttttaat gttctgtaca tcgcgcaaaa tacgttccgc aatatccaaa
     6661 accaccttgc ccggctgcga gaccgaaacc acgcgcttgc cgctgcggat aaaaatctga
     6721 atgccgattt cttcttccag caatttgatt tgtttggaga tgccgggttg tgatgtgaat
     6781 aaggcttcgg ccgcttcgga aacgttcagg ttgtgccggt aaacttctaa ggcgtatttc
     6841 aattgttgta atttcatggc gggtcggtgt gggtctgtgt cgggtggctg aacattgttt
     6901 ataatttatc atattttctt gccggtacgg tatggggctt tgccgttgtg tttgttgttt
     6961 ttgtgcaacg gcaatcgtgc gatatggaaa aaatccccct aaagtaatga cacggaattg
     7021 atttttcggc atgatagact atcaggaaac aggctgtttt acggttgttt tcaggcgttg
     7081 agtattgaca gtccgccccc tgtttcttta tagtggagac tgaaatatcc gatttgccgc
     7141 catgtttcta cagcggcctg tatgttggca attcagcagt tgcttctgta tctgctgtac
     7201 aaatctaatg agggaataaa atgaccaaac agctgaaatt aagcgcatta ttcgttgcat
     7261 tgctcgcttc cggcactgct gttgcgggcg aggcgtccgt tcagggttac accgtaagcg
     7321 gccagtcgaa cgaaatcgta cgcaacaact atggagaatg ctggaaaaac gcctactttg
     7381 ataaagcaag ccaaggtcgc gtagaatgcg gcgatgcggt tgccgtcccc gagcccgaac
     7441 ccgcgcctgt cgccgttgtg gagcaggctc ctcaatatgt tgatgaaacc atttccctgt
     7501 ctgccaaaac cctgttcggt ttcgataagg attcattgcg cgccgaagct caagacaacc
     7561 tgaaagtatt ggcgcaacgc ctgagtcgaa ccaatgtcca atctgtccgc gtcgaaggcc
     7621 ataccgactt tatgggttct gaaaaataca atcaggctct gtccgaacgc cgcgcatacg
     7681 tagtggcaaa caacctggtc agcaacggcg tacctgcttc tagaatttct gctgtcggct
     7741 tgggcgaatc tcaagcgcaa atgactcaag tttgtcaagc cgaagttgcc aaactgggtg
     7801 cgaaagcctc taaagccaaa aaacgtgagg ctctgattgc atgtatcgaa cctgaccgcc
     7861 gcgtagatgt gaaaatccgc agcatcgtaa cccgtcaggt tgtgccggca cgcaatcatc
     7921 accaacacta aggctaggta atatcttgcc gatgcatgag gttagcggat tttgtaccgg
     7981 gtactgttgc aatattcgtg aaacgtcggc cggtatcgat gatgtgaaac aaaccccgct
     8041 tttgcggggt ttgttttttt gggtggtttt ctgaaacggc tatcgtcaga atcggggtgc
     8101 aggttcggat tcggattcag attcatgttt gtgtcccatt gccgcgcttt atagtggatt
     8161 aacaaaaatc aggacaaggc gacgaagccg cagacagtac aatagtacgg caaggcgagg
     8221 caacgccgta ccggtttaaa tttaatccac tatatcggtt gaaactctga ttttaaggcg
     8281 gtaggatgtg ggtttgccca tagcaaggga atcctttctg tatcaagccc cgaaagggat
     8341 aattcataca aattcacgcc tttccccctc attgggaaat ggatggaatc gtgcccgatg
     8401 tgtgcggcac tgtatgccgg atatggtttt atcatcatcc cttttcggtt gaaaccccgc
     8461 cagttgcagc gattgagcct aatcggtggc ggaagttgcc gctttgcatt cggggcggcg
     8521 tgcagtgcgg tgctttgata tgccgtttgt gtgttgaaac agggtggtcg gtgcatacgg
     8581 gtacggtatg gctaaagcta aaagtgaaat acgctgaaac actgaatgag ccgctttatt
     8641 gtttgtacga cctttgctgc tttgctatga tttaaattgg attcgcacgc cggatatttt
     8701 gggatatgaa agaattcgac ttcatcaaac ggtatttgca aacaggtacg gataaggatg
     8761 tcgtattggg cataggcgac gatgcggcga ttgtccgccc gcgcgaaggc ttcgatttgt
     8821 gtttcagtgc ggatatgctt ttgaaggaca ggcatttttt tgcagatgtc aaacctgaag
     8881 gcttggcttg gaaggttttg gccgtcaata tttcagatat ggcggcgatg ggtgcgatac
     8941 cgcgttgggt gctgctgagc gcggcgttgc ccgaattgga tgaagtatgg ctggaacggt
     9001 tttgcggcag ctttttcggt ttggcaaaaa agtttggcgt aacgttaatc ggcggcgata
     9061 cgaccaaggg cgatatggcg ttcaatgtaa ccattatcgg cgaattgccg aagggtaggg
     9121 cgttgcggcg tgatgcggcg gttgcgggcg acgatatttg ggtgtcgggg cgtgtcggta
     9181 tggcggcggc ggctttgaac tgccgtctga aacggtgtgt gttgccggat gatgtgttcg
     9241 ccgaatgcga acaaaagctg ctccgtcccg agccaagggt tgggctgggg cttgcgctgt
     9301 tgccgtttgc ccgggcggca caggatgttt cagacggcct tgcgcaagat ttggggcata
     9361 tcctgaccgc ttccggcgtg ggggcggaaa tttgggccga ttcgctgcca tctttatctg
     9421 tattgaaaga tattttgccc cgagcgcaat ggctgtctta tactttggcg ggcggcgacg
     9481 attacgagct ggtttttacc gctccggaaa gttgccgcag ccgcgtgctt gatgcggcgg
     9541 aacggtgcgg cgtgccggta acgtgcatcg gcaaaatcaa cggaggatgc cgtctgaagg
     9601 ttttagatgc cggcggcagg gaattggaac tacattcttt aggattcgat cattttggct
     9661 gattttaaac ctgactttgc gtggctgttg aaacggccgt tgtgtttttt ggctttcggt
     9721 ttcggcagcg ggctggctcc gtttgcgccg ggcgcattcg gcactttggc ggcactgcct
     9781 ttggcgtttg tgctgatttt gctcggcata gacgggctgc tgctggcttt tgtgtgtatc
     9841 gtgctgttta tgtggggcat acgtatttgt gcgtgtgcgg aacgtgaaac gggtgtcagt
     9901 gaccacggtg ggattgtttg ggacgagatt gtcgccatgc tgtttgtgct ggcgtttgtg
     9961 ccgttcaggt ggacgtggtg gctggcggca tttgttctgt tccgtctgtt tgatgcgctc
    10021 aaaccgcccc ccgtcggttg gtttgacaag aatctgcacg gcggtttggg cattatggcg
    10081 gacgatatgg cggctgcggt gatgaccttg attgtcttga ggattgcaat gctgttttaa
    10141 acggggctgc cttgtaaaaa tgccgtctga aagcctttca gacggcattg tttcggaggt
    10201 taacgcgtta ccggtttgta tttgatgcgt ttcggtttcg cgccttcttc gccgagtcgg
    10261 cgtttcttgt cggcttcgta ttcttgatag ttgccgtcga agaacaccca tttggagtcg
    10321 ccttcacacg ccaagatatg cgtggctatg cggtcgagaa accagcggtc gtgcgaaatc
    10381 accatcacgc tgccggcaaa ttccaacaat gcgtcttcca acgcgcgcag ggtttccacg
    10441 tcgagatcgt tggacggttc gtccagcagc aacacattgc cgccgcccaa caaggttttt
    10501 gccaagtgca gacggccgcg ttcgccgccg gaaagctgcc ttgcgatttt gctttggtcg
    10561 ctgcctttaa agttgaagcg tcccaaatat tggcgggcgg ggatttcaaa ctgtccgacc
    10621 tgcaaaatat cgcgaccttc ggcaatgttg tcgaacacgg ttttgtcgtt ttgcaaacct
    10681 tcgcggcttt ggtcaatcaa gctcattttc acggtttgcc cgattttcac ttcgcccgaa
    10741 tcgggctgct ctttgcccgc aatcattttg aacagcgtcg atttacccgc gccgttcggg
    10801 ccgatgatgc cgacaatcgc gcccgccggc actttgaagc tcaaatcgtc aatcagcact
    10861 ttatcgccga acgatttgga aacattcaca aattcaatca cttcgttacc caaacgctcg
    10921 gcaacaggga taaagatttc ctgagtttcg ttgcgttttt ggtattcgta gttgctcatt
    10981 tcttcaaaac gcgccaaacg cgctttgggc ttggcttggc ggcctttggc attttggcgc
    11041 acccattcca attcctgctt catcgccttc acgcgcgcgg cttcggattt cgcctcgttt
    11101 tccaagcgtt tttctttctg ctccagccaa gacgagtaat tgcctttcca cggaatgccg
    11161 tgtccgcggt cgagttccaa aatccattcg gcggcgttgt cgaggaagta gcggtcgtgc
    11221 gttaccgcga ccactgtgcc ggggaagcgc acgaggaatt gctccagcca ttccaccgat
    11281 tccgcatcca agtggttggt cggctcgtcc aagagcagca tatcgggctt gctcaacaaa
    11341 agtttgcaca aagcgacgcg gcgtttttca ccgcccgaaa gattgccgat tttggcatcc
    11401 caatcaggca ggcgcagcgc gtcggcagcg atttccaatt cgtgttccgc accgccgcca
    11461 gttgacgaac ccgccgcgat aatcgcttcc aagcggcctt gttcttccgc cagcgcgtca
    11521 aaatccgcat cgggattggc gtactcggca tacacttctt ccaaacgttt ctgtgcggca
    11581 gccacttcgc cgaaaccgct ttccacttcc tcgcgcacgg ttttttccgg atcaagctcc
    11641 ggctcttgcg gcagatagcc gattttgatg tcgcccatcg gcacggcttc gccctcaaat
    11701 tctttatcca cgcccgccat aatccgcagc acggtggact tgcccgtgcc gttcaagccc
    11761 aagaggccga ttttcgcgcc ggggaagaaa gaaagggaaa tatctttaat gatggttttc
    11821 tgcggcggca caaccttgct cacgcgcagc atagaataga cgtattgttg ggacatagtt
    11881 ttctcgattc ggtcaaacaa atttcagacg gccattttaa ccgataattt gatttaagcc
    11941 agtttatccg cgaaccggta ttgccaaaat cgggcaggat tcataaaatc cgcctatccc
    12001 tttgaaatta tacagacaaa aaaataataa tgatagggga tcgccgcgcc ggcaaccatt
    12061 tcggattttc caaagcaaat acttttcaaa tcaaaaaaaa ggatttactt tatgtcggaa
    12121 tatacgcctc aaacagcaaa acaaggtttg cccgcgccgg caaaaagcac gatttggatg
    12181 ttgagcttcg gctatctcgg cgttcagacg gcctttaccc tgcaaagctc gcagatgagc
    12241 cgcatttttc aaacgctagg cgcagacccg cacaatttgg gctggttttt catcctgccg
    12301 ccgctggcgg ggatgctggt tcagccgata gtggctacta ctcagaccgc acttggaagc
    12361 cgcgcttggg cggccgccgc ctgccgtatc tgctttacgg cacgctgatt gcggtcatcg
    12421 tgatgatttt gatgccgaac tcgggcagct tcggtttcgg ctatgcgtcg ctggcggcct
    12481 tgtcgttcgg cgcgctgatg attgcgctgt tggacgtgtc gtcgaatatg gcgatgcagc
    12541 cgtttaagat gatggtcggc gatatggtca acgaggagca gaaaagctac gcctacggga
    12601 ttcaaagttt cttagcgaat acggacgcgg ttgtggcagc gattctgccg tttgtgttcg
    12661 cgtatatcgg tttggcgaac actgccgaga aaggcgttgt gccacaaacc gtggtcgtag
    12721 cattctatgt gggtgcggcg ttactgatta ttaccagtgc gttcacaatc tccaaagtca
    12781 aagaatacga cccggaaacc tacgcccgtt accacggcat cgatgtcgcc gcgaatcagg
    12841 aaaaagccaa ctggttcgaa ctcttaaaaa ccgcgcctaa agtgttttgg acggttactc
    12901 cggtacagtt tttctgctgg ttcgccttcc ggtatatgtg gacttactcg gcaggcgcga
    12961 ttgcagaaaa cgtctggcac actaccgatg cgtcttccgt aggccatcag gaggcgggca
    13021 accggtacgg cgttttggcg gcggtgtagt cggttgcggc ggtgatttgt tcgtttattc
    13081 tggcaaaagt accgaataaa taccataagg cgggttattt cggctgtttg gctttgggcg
    13141 cgctcggttt cttctctatc ttcttcatct acaatcaata cgcactcatc ctgtcttata
    13201 tcttaatcgg catcgcttgg gcgggcatta tcacttatcc gctgacgatt gtggccaacg
    13261 ctttgtcggg caaacacatg gatacttatt tgggcctgtt taacggctct gtctgtatgc
    13321 cgcaaatcgt cgcttcgctg ttgagtttcg tgcttttccc gatgctgggc ggccatcagg
    13381 caaccatgtt cttggttgca ggcgcagtct tgctgctggg agccttctca gtctgtctga
    13441 ttaaagagac ccacggcggg gtttgagcga tgagcgatac ccccgctacc cgcgatttcg
    13501 gcctgatcga cgggcgggcc gtaaccggct atgtgctgtc caaccggcgt ggtacgtgcg
    13561 tcttcgtgct ggacttgggc gggattgtgc aggaattttc cgttttggca gacggcgtgc
    13621 gcgaaaaccc cgtggtgtcg ttcgacgatg cggcttccta tgcggacaat ccgtttcaga
    13681 ttaacaagca gatagggcgc gtggccggac gcatccgcgg tgcggcgttc gacatcaacg
    13741 gtaggactta ccgcgtggag gccaacgaag gcaggaacgc gctgcacggc ggttcgcacg
    13801 ggctggccgt tacccgtttc aacgcggtgg cggcagacgg ccgacggtta tcccaacgat
    13861 ttggatattt cctaccgctt ggacgaggat ggccggctta ccgttaccta tcgcgccacc
    13921 gcgctcggcg acacggtgtt cgacccgacg ctgcacattt actggcggct ggacgcgggc
    13981 ctgcacgatg cggttctgca tattccgcag ggcggacata ttccggccga tgccgaaaaa
    14041 ctgcccgtct taacggtttc agacggcctc gaagtatttg atttcagccg gcccaagccg
    14101 ccggatgccg ccgttgccgc cctgcgccgc gaaacgggcc ggttttgacg acgcttaccg
    14161 cgtgccgtcc gatataggcc gtcccgccgc cgtgttgcaa gccggacgcc gccgtcgtat
    14221 cagcatatac agcgaccgca acggcttggt catctttacc gccgccccgc aggatttcgc
    14281 gcggcacgat gcgggcgttt acgacgcgct ggcgaccgag gcgcagacgc tgcccgacag
    14341 cctgcattgg cccgaattcg gcaatatccg tctgaacaag ggtgatacta gggaggcgac
    14401 gattgcttac ggcatcgaat ccctttctta ggagcttcct aacaccggtt tcagacgacc
    14461 tttttatagt ggattaacaa aaaccagtac ggcgttgcct cgccttgccg tactatctgt
    14521 actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccacgcctt gccgtactat
    14581 ctgtactgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatccact ataagatttc
    14641 accattccct caaatcaatc caaacaggag ctttacgatg tacacaagaa ttatggaaat
    14701 cagcccttgg acgctgcgtt cggcaaaact ggaaaaagaa cacaaacggc tgcaagagag
    14761 cctgaccagc ttgggcaacg gctatatggg tatgcgcggc agctttgagg aaacctactc
    14821 cgccgacagc cacttgggca cctacatcgc cggcgtgtgg tttcccgaca aaacccgcgt
    14881 cggctggtgg aaaaacggct atcccaaata tttcggcaaa gccatcaacg cgctcaattt
    14941 cagcaaagtc aaaatctttg tcgacgggca agaggtggac ttggcgaaaa acgacgttgc
    15001 cggcttctcc gtcgaactgg atatgcaaca cggcgtgttg cgccgctcgt tcaccgtatt
    15061 cggtgtgcgt ttcgatgtgt gcaaattcct gtcggtcgcg caaaaagagc tggctctcac
    15121 ccgctgggaa gccgtgtccg ttgacggcaa aacccaccaa gtccgcatcg attccatcat
    15181 cgatgccgac gtgaaaaacg aagactccaa ctacgaagaa aaattctggc aggtattgga
    15241 caaaggcgtt tcagacgacc gctcctacat tgccgcccaa accgtcgcca accctttcgg
    15301 tgtggaacaa tttatcgtca acgccgggca aaccttcgcc ggcagcttca aagccttcgg
    15361 cggcagccaa accgattggc aggtctctaa ttcttttgaa gccgaagtcg gcggcacgcc
    15421 cgaaaccttt gaaaaacgcg tgattgttac caccagccgc gattatcagg gcttggaagc
    15481 agtgaaagcc gccggccgcg ccttatctga aaaaatcgca ggtgttgcgt ttgaaacctt
    15541 gctggatgcg cacaaagcag gctggcagca tcgttgggaa atcgccgacg tggtcatcga
    15601 aggcagcgac gaagcacagc agggcatccg cttcaacctg ttccaactgt tctccaccta
    15661 ctacggcgaa gacgcgcgtc tgaacatcgg ccccaaaggc tttaccggcg aaaaatacgg
    15721 cggcgcgacc tattgggaca ccgaagccta cgccgtgccg ctctaccttg cactggcgga
    15781 acccgaagtt acccgcaacc tgctgcaata ccgccgcaac caaattgccg caggtgcagc
    15841 ataacgcacg cgaacagggc ttggcgggcg cactctatcc gatggtaacg tttacgggca
    15901 tcgagtgcca caacgaatgg gaaatcacct tcgaggaaat ccaccgtaac ggcgcgattc
    15961 cttacgccat ctacaactac accaactaca ccggtgacga gtgctatctt gccaaagaag
    16021 gcttggaagt cttggtcgaa gtgtcccgct tccgggcgga ccgcgtccac ttctccaaac
    16081 gcaacggcaa atacatgatt caaggcgtaa ccggtccgaa cgaatacgaa aacaacatca
    16141 acaacaactg gtaaaccaac accctcgccg catgggtatt gggctacacc cgcgaagcct
    16201 tggcgaaata cccgcgtccg ggttcgaacg tgagcgccgc cgagttggaa aaatgggcgg
    16261 acatcagcgc gaatatgtac cgtccgcatg acgaagaact cggcgtattc gtgcagcacg
    16321 acggcttcct tgacaaagac atccgccccg tatccgcgct ttcgcccgac gatttgccgc
    16381 tcaaccagaa atggtcgtgg gacaaaatcc tgcgttcgcc cttcatcaaa caggcggacg
    16441 tattgcaagg catctacttc ttcggcgacc gtttcgacat ggacgaaaaa cgccgcaact
    16501 tcgacttcta cgaaccgatg accgtgcatg aaagctcgtt gtctccatgt atccactcca
    16561 tcctcgctgc cgaactgggc aaagaagaaa aagccgtgga aatgtaccgg cgcaccgccc
    16621 gcctggactt ggacgactac aacaacgaca ccgaagacgg cctgcatatt acttctatga
    16681 ccggctcgtg gctcgccatc gtccaaggtt tcgcccaaat gaaaacttgg ggcggcaaac
    16741 tcagcttcgc accgttcctg ccgagtgcgt ggacaggcta tgccttccac atcaactacc
    16801 gcggccgtct gattaaagtc gccgtcggca aaaaacgtcg tcttcaccct gctcaaaggc
    16861 gatccgctcg aattgcaggt gtacggcaaa gatatcacgc tcaacggcag ccacaccgtt
    16921 gcattggaaa aataaggagg gcgcaaaatg acttttaccg cagtgctctt cgatctcgac
    16981 ggcgtgatta ccgataccgc cgaataccac tacctcgcat ggaaaaagct tgccgaagaa
    17041 ttgggcatcg gcatcgaccg caagtttaac gagcagctca aaggcgtgtc gcgcgacgat
    17101 tcgctcaaac gcatcctcgc gcacggcggc aaaaccgtcg gcgaaaccga gttcgccgaa
    17161 ctgacctgcc gcaaaaacga caactactta gagatgattc aggcagtcaa acccgaagac
    17221 gtgtaccccg gcattttgcc gctgctggaa gcattgaggg caaacggcaa aaaaatcgcc
    17281 ctcgcgtccg ccagtaaaaa cggcccgttc ttgctcgaac gcatggggct gacccacttc
    17341 ttcgacaccg tcgccgaccc tgccgccgtc gcgcattcca aaccctcccc cgacatcttc
    17401 cttgccgccg ccgagggcgt gggtgcggac atacgccgct gccggcgtcg ccgccatcaa
    17461 agcctctggc gctttgccca tcggcgtggg caaaaccgaa gacttgggca gcgacatcgc
    17521 gctggtgtcc gacaccgccg agctgaccta cgcctacttg caaaatgtgt gggaacagtc
    17581 gggcagataa aattctaagg aagtaaaaag gccgtctgaa cagtgtttca gacggccttt
    17641 ctgcttttag aacagaatga taacccgaga cctttgcaaa aatccccaaa atcccctaaa
    17701 ttcccaccaa ggcatttagg ggatttccca tgagcacctt cttccggcaa accgcccaag
    17761 ccatgactgc caaacacatc ggccgcttcc cgctatcgga gttggaccag gtgattgatt
    17821 ggcagccgat c
//
LOCUS       DBJGGI010000040        17627 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.040, whole genome shotgun sequence.
ACCESSION   DBJGGI010000040 DBJGGI010000000
VERSION     DBJGGI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17627
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.040"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            445..612
                     /locus_tag="ACSIGJ_001345"
     CDS             445..612
                     /locus_tag="ACSIGJ_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330191.1"
                     /translation="MVNEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNLDFSQW
                     VKSIIKEKIKK"
     gene            772..1617
                     /locus_tag="ACSIGJ_001346"
     CDS             772..1617
                     /locus_tag="ACSIGJ_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRO-N domain-containing protein"
                     /protein_id="HHQ1330192.1"
                     /translation="MNAVQVLNFQQNSVRTVADNKGELWFLANDVCEILGYTNPRRTV
                     DLHCKSRGVTKRYTPTASGEQEMTYINEPNLYRLIIKSRKPAAEAFEEWVMETVLPAI
                     RKTGGYQITPKTTADDRTGLRQAVAALVGRKGIDYSSAYSMIHQRFNVESVEDLPAGK
                     LPEAVAYVHALTLHTGLTGEVLDAPPKAEPKLPIDGNSLADIAAMVYYGTWMIESGKD
                     ISAPLKQLGCRQAVTMWTVWHETRSILKRSAAALEVLRGYADKDASDRIAACLEGIYG
                     KATVR"
     gene            complement(1654..1803)
                     /locus_tag="ACSIGJ_001347"
     CDS             complement(1654..1803)
                     /locus_tag="ACSIGJ_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330193.1"
                     /translation="MSAEEFCRKQIAYWLNESRKASDNADLKAFEFAGREPADYREML
                     KRYAA"
     gene            complement(1806..2480)
                     /locus_tag="ACSIGJ_001348"
     CDS             complement(1806..2480)
                     /locus_tag="ACSIGJ_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330194.1"
                     /translation="MNLKALIARFRVLANDKAEPYFWSDEEVSGWLNDAVHEACLRGR
                     LLHSDDAFVTDVEKGRPLYAYAAGGFAAGYAYEIDSIRFVSDGKPVCLKLVSPEAADV
                     CAPGWRDGAQTGLPVYAVQGDGKLTLAPAPDRDGRLFAGGYCLPRDMAGDGDEPEINS
                     IHHRNLVYWALAEAFSIPDAETFDPQRSESARRRFELYFGLPADSDLRRITREDAPHL
                     NRHFWI"
     gene            complement(2477..2962)
                     /locus_tag="ACSIGJ_001349"
     CDS             complement(2477..2962)
                     /locus_tag="ACSIGJ_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330195.1"
                     /translation="MKRRPGICLCGVAVFDNAVRTAAGGWMDKVFIKYIGGRAVWRDR
                     IYHTGLVFEDGQVREVSAGAAAKLLRHGDVFAAVPGKRVEKADDTEALEKAGASELER
                     ETAAFDAVQDVILQINRMGKDELELYAKANYGQGLDKRKSAENLRGDVVRMVRQFGIA
                     Q"
     gene            complement(2968..3441)
                     /locus_tag="ACSIGJ_001350"
     CDS             complement(2968..3441)
                     /locus_tag="ACSIGJ_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330196.1"
                     /translation="MAEIYVRNNGGNRFGGVPYGNLAAEHYRIVAKQDGAILGADAYG
                     PPKDGDVLALGVLEQGFRLDDAQIIVKTAMSSGITADVGFAYADGADDAHVPQDAAYF
                     ASGADFASAGRIRCQSAKLVTLPKQALLTVTLKGAENKKAADIDILIYGEKFGQL"
     gene            complement(3496..4791)
                     /locus_tag="ACSIGJ_001351"
     CDS             complement(3496..4791)
                     /locus_tag="ACSIGJ_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HHQ1330197.1"
                     /translation="MAQKTNTAYGDPQAMMRQAAGLFAMHMQRNSTLNRLAGKMPAGT
                     AGAEATLRKQTTQHMPVVRCQDLTRGMGDEIRFNLVNPVSALPIMGDNTAEGRGVGMS
                     LSEAGLRVNQARFPVDGGGTMTNQRSPADYRALIRPAAQSLMDRYADQTLLVHMAGAR
                     GFHDNIEWGVPLAGDPKFNDYAVNPVKAPSKNRHFTASGDAVTGVGQDGGELKIASTD
                     LFTMDTVDSMRTVLDQIPLPPPIVKFEGDKAAGDSPLRVWLLSPAQYNRFAADPKFRQ
                     LQASAIARASQANQNPLFLGDAGLWNGFILVKMPRPIRFYAGDEMKYCADKFSEAESG
                     LKIPASFADKFAVDRSVILGGQAVLEAFANTGKHGGMPFFWSEKELDHGNRVETLVGT
                     IRGVAKTRFAVDVGGGAKEITDYGVTVVDTVVPLHGGIR"
     gene            complement(5417..12721)
                     /locus_tag="ACSIGJ_001352"
     CDS             complement(5417..12721)
                     /locus_tag="ACSIGJ_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLxRFG domain-containing protein"
                     /protein_id="HHQ1330198.1"
                     /translation="MSDLVRYDPLEHGRLAGGLKEYRGFTQKDARAAADDTALTRGFK
                     NSMRSARMGWNALTGDKEELGRLKAEDMDYRKIQEGRKSQARRELGEAWEKGGGVGGG
                     LSNVWGELKKDWREKGLDGALEDVGEMGGAVLEQAPNALVPIATTTAGGILGALAGGN
                     AAVGAYAGATLGNTLMEYGGQLDRAAEAAGVDPADKDAVMAFIGRGAPGALKNAAVKG
                     AVVGAADMAAMKLGGSILNMGKKAAGKAALEKMGVAAADKAAVAAAKGTPEFAALAKE
                     SAKGGLGGAARHAAAYATESAGEFAGEYLGTGLANGEWDEKGAALEAFSSLGHSAVGF
                     AGTKAYAAVTDPLRPPAGRKAGAQGGIGGNRKAGRPAPEGAQALRAAAQAAADGGAEQ
                     GGAGFDTAHHDQSHPALRQFADRTKQEEAGRFFSGPADGNTPHAEELARGTEKQPDVS
                     GIPSEDGAEFLDTGVMPDGLARQYADMAAKYRAKPSEAVGIDPDDGAVSAAAALAADS
                     GAAVPSAVSDDMEARSVADDAPSGRSADADRGGVPSAYGNVRPGGAPRGAASVAPGGS
                     AAAASGGIARVSPLPAGQYFDGLDTRGRKALAKEAGLDIKGVADFGQIAAPVRRKIEQ
                     AYHARIEADYQAASEAKQGYLPPPVRMADAVPVPKKGFSVPADALDKESRRRFDALPE
                     GVRRHAQTVADYTADGIMRREAGMADMRGHYPEGLAESAGAVRAYRAEHPESADVLDR
                     LNRAVYGYRRNNGWSVPLLSREGERLQGVRTALPDDGASEAVVGGGRGLTRALPTEDK
                     GLAQDVRQDVRQGLTQGGRGLTPDAGADANAAALQGLPGSAAVASGNAPARRQNLQVR
                     ARAEGAAPGLSASENLAGTDGGKRAPVAGKRPDTVLPVLNPQVAASAGRVSPKKRMAD
                     AAADRRRPEKAGVPLGGGEYRFEHTDRRHIDALAGVPGRPGKGGMPEEFADMAGPSNS
                     DGLVSDGRRYLKGREAETLRAGGLSEAVPSEPGRDYRPTQEARAPARVMARPRDAAAD
                     GKPAGRAQPARAKDTPVAGKAAAAKNAATEKPSSDKVRNIESGKSHFDGGKGKSAAAQ
                     GAATEKPSSDKAAKPETFAKTASDNPEEARRKARVLQGGPVYTVKERQAPQGFKALRE
                     HAENIKKRLAESIGGLAERVDVAAVSETAPDKAQMLLSQRVEGWFDGRTGKITLVAEN
                     LTPERAVWAAWHELGHRGFAADGFAKYREELERADGNGLIRRIADAVQEGREGTGDAA
                     ASVRSAAVEEAVAELYAAQRTGGWAGIENRYGVKVGNGLKRGIAGVLARIGALLRRVL
                     QRLAGKAGGAMSDADVFAMLADLHGNVEGARDAPWDGNHRAVMFARAEDGAAERSKSE
                     SLEKLRRAETIRISGREVPEGGNLREYKRNALEYGKSLRGPYVNKDTGREISLGRSGI
                     TEILRHDYKDAEHLQSIAAIPQIIENAVYIDTLPNEDLAKNGDIQGYEYYVSGLNVGG
                     ADYTVRAAVAVSRNGNRYYDHKLTKIEKGNLLSLLDRVSTTGASESKSPLSGIDDKRL
                     LQILQDKDAGKGGIADFDTEAVRFSRAANIGAAISRITGKKSDLRNALKDRWDASKGI
                     QLQFLGRRQIEDIYGGVLDGLKEYGRLSELFGADANKAVTEADKVVREWGRLKEEDAK
                     ALADLMHDATLAKVDADPLMRKDARGRLDGIRTALDIADGKIAKARAAVASAGARTAR
                     ADAAYNKAQRAADKAAYALEKAQEKHGREILADEADMRLRRLFYADSEAKRALRRAGA
                     DAAAESRAKTDAVRMLEQARADVKRLEKDEVGAQKALEGLALLNRRFAGLPDAAQRVY
                     RKARDDYRAHFGQVRDALAERLARAGQDAETVRRLKERFDNELGGVYFPLARFGDYLV
                     VVKDADGNSANVSRAETLSEAEKLRDALKADFGPGFKVSPVMKSRDYIRSRDAVGSGF
                     MKELGEAVGRMDLDPAQRARLNDTLTQLYLNSLPDTSWAKHGIHRKGVPGFSDDARRA
                     YAQNMGSGANYLAKLRYADRMAEQLDVMQDFVDGRKYEEGFDQRQLQRVADEMRKRHE
                     AVMNPNPSKLAQALTGFGFLWMMGMSPASAVVNLSQTAMVAYPVMAAKWGYAGAAREL
                     LRASKQIGLRFGEKFNTIEDSLNGDEKAAFRKAADYGVIDLSQAHDLAGVANGDPGLA
                     GSAWQKVMDKAAWLFHHAEKFNRQVTFVAAYRLAKRAGADSEAAFEQAKKATYDGHFD
                     YAAQNRPRFMMGNAAKVVFLFKQYSQNILYALGRNAYLAFKGDKEARKTLAGLLVSHA
                     MASGILGLPFVSTLLAVASMLGSDDDDPWDAEAALRNMLADTFGDKAGEVLAKGFSRL
                     TPLDVSGRLGLDQLVFPDIQDGLEGKKWAESLVVGSTGAVVGAGIGAADGVRTRSSVP
                     RTANTLSLMKSW"
     gene            complement(12718..13914)
                     /locus_tag="ACSIGJ_001353"
     CDS             complement(12718..13914)
                     /locus_tag="ACSIGJ_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330199.1"
                     /translation="MGLFEPSAGDFWEMKEKEKKEKARKGAEERERAAAQAHRADAVR
                     RTVANYEAGPARYRNVMDLSRNNIEDGARRLRRAGAFERGADAGLGFSGGDKALSPDA
                     RAGADFARRDTRPTDAGGRTPPPLGFDGNVYRDGKPVRDFDAQRPLVSAGPDALSPEE
                     RELYRRATTPHAGALNGQLTAAQLNAARGIVAEHNKNAAVRELGRERLAAAAAENAAN
                     REAVLQEGRFDAAVKANEGALNREMAQRNADRAFDVQQAELGMKRQGFEMKREADALE
                     LEDRKRIADLTRAYGFAKSDGQRGEIARQIDALNGKFERQGEKGFDPNVFKTISYEVA
                     DPDTGLTAKREGIVDLRTGKPLDVEFAGEREKRYAQLGFKPNGQKTAGGKIIYENEKG
                     EKRVEQ"
     gene            complement(14151..15362)
                     /locus_tag="ACSIGJ_001354"
     CDS             complement(14151..15362)
                     /locus_tag="ACSIGJ_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HHQ1330200.1"
                     /translation="MTGKTVDLKLPAKLDGLFKPCRYKVMYGGRGGGKSHGAASALLA
                     LGAQRPLRILCVREIQKSMRDTVHRLLKDKVAQLGLGHFYEITDFEIRGANGTLFVFS
                     GLQSHTVDSIKSFEGIDIVWVEEGHGVSKKSWDVLTPTIRKEGSEIWITLNPDMETDE
                     TYRRFIAMPSEDTWLCEINWRDNPWFPEALNRERLKAQRSMNKEDYGNIWEGRPRMVS
                     EGAVYRHEIQDAFHSGRVTLVPYDSSLPVHTVWDLGWNDAMTIGLVQRDLTSVRIIGY
                     IEDTHRTLDWYVAELEKLPYRWGTDFLPHDGRTRNFQTGKSTMEILTGLGRKSVFVQN
                     ATGIEEGIRAARGDDPGFPVPAMPPETQIPPEGIPEAYGADTGPMTAVPPKSANHAQT
                     GMETPTVSDNL"
     gene            complement(15343..15882)
                     /locus_tag="ACSIGJ_001355"
     CDS             complement(15343..15882)
                     /locus_tag="ACSIGJ_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330201.1"
                     /translation="MPRLSDEDWRKVELDYRRGVLSIAEIGRKYNVSAQHVGRVAKER
                     VWTRDLNDEVQAKARAMVLSADKNGNAPDYADFNLKQVTDAEAKRVAAVQRRHRNLAE
                     NLSKSAEQIVAELYGSPEDTFTKARMFQTVAAGFKVLVEIERKSYGMDTAESKISESA
                     KTAGIRIEFVGPEDDGKDG"
     gene            complement(15882..16304)
                     /locus_tag="ACSIGJ_001356"
     CDS             complement(15882..16304)
                     /locus_tag="ACSIGJ_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330202.1"
                     /translation="MSGLSDIDKVYQGVVDLHNAEQVVSREALAETTGLKMSEITKFT
                     KLLVEHGKIYRVTRGIFKPAIGFGETRPVSVSVLDSGMGVLEIGDTVLHLNPQEMRSL
                     GALMSGFGQQFSSIQMGREFSVLRNYLECSAKNGRLDL"
     gene            complement(16369..16887)
                     /locus_tag="ACSIGJ_001357"
     CDS             complement(16369..16887)
                     /locus_tag="ACSIGJ_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HHQ1330203.1"
                     /translation="MGLTQEVLKELLRYDDNTGKLYWAERPRKYFNSGLHYKSWNTGF
                     SGKEVFLYKGRLGYLKLKIFKKQYNAHRLIWLFVYGKHASSIDHINRDKTDNRISNLR
                     DVTHAENMKNRGKFKNNTSGHTGVYFHKPSKKWQARIMVNRKNKILGLFEHIEDAVKA
                     REAASKDFGFVV"
     gene            complement(16878..17261)
                     /locus_tag="ACSIGJ_001358"
     CDS             complement(16878..17261)
                     /locus_tag="ACSIGJ_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HHQ1330204.1"
                     /translation="MNGAEFTLTPQNKKQVMRSIWDSPDGWFENGNLEITIRPRKSKR
                     SVEQNRRLWFLYREISEKVFIDGRRFSQDVWHEFLKRKFIGCIEMPNGQLMGISTTKL
                     SVREMSEYQEKIISWASMEHGVLWD"
     gene            complement(17258..17563)
                     /locus_tag="ACSIGJ_001359"
     CDS             complement(17258..17563)
                     /locus_tag="ACSIGJ_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease domain-containing protein"
                     /protein_id="HHQ1330205.1"
                     /translation="MSSAIRKAAKGGQCTPNIAGVCNDNPETVVLCRFPGETHGAGLK
                     SGGLGAGFGCGCRRGAIDGRGAGLSREDKEFYMRRSQLRTIRRLEALGVVGVKGRLK"
BASE COUNT         3751 a         6211 c         4004 g         3661 t
ORIGIN      
        1 gaattatatt tataaaaaaa ggtgcaaata aggtgcaaat aaaattagtt tttcccaatg
       61 ggttaaagaa caaatccgaa aagagttctt aaaatagccc gaccgccgcc aagccgcaaa
      121 cttggcggct tctttatgcc gtccgaaagc ccttcagacg gcatttttct ttgattttgt
      181 tgggggaatg gttgactttg aacgtgtcca cgtttataat tcaattcatc ggcgcatcgg
      241 gtgccgatac gggaaaagcc ccaccgaagc ggggcttgta cagggagtag gaaaatgaag
      301 ctcatccttc aaattctcat cctggtttta atcttgatgc tggcaggcga tacgcctatt
      361 agcggataaa accggctggg gcggtagccg ccgccccagt ccctatctta caaaaggcag
      421 ttcaaaaaat caaggagttt gcctatggtt aatgaaaaac tggcagaaaa ccgaaagcgt
      481 tacgagcaaa aacgagtcat taaaaaagtc tcgtttaatg cggaaacaga aaaagagctt
      541 ttggaatatg cccaaaatct cgatttttcc caatgggtta aatcaatcat aaaagaaaaa
      601 atcaaaaagt aattgacttt gaacgtgtcc acgtttatta ttcttacaac ggcacagccg
      661 ttaatggaaa accgaaccaa gcgggaactt ggttcggtta agtgaaaagc agttctagga
      721 aaactctttt cgcgtctttt aaataacctc taaaagaaag actattctat catgaatgct
      781 gtccaagttt taaactttca acaaaactcc gtccgtactg ttgccgataa caaaggcgag
      841 ttgtggtttt tggcaaacga tgtttgcgag attttaggct acaccaaccc tcgccgaacg
      901 gttgacctgc actgcaaaag caggggcgta acaaaacggt acacccccac cgccagcggc
      961 gaacaggaga tgacctatat taacgagccg aatctctacc gcttgattat caaatcccgc
     1021 aagcctgccg cagaagcgtt tgaagaatgg gtaatggaaa ccgtcctgcc cgccatccgc
     1081 aaaacaggcg gctaccaaat tactcccaaa accaccgccg acgaccgtac cggattgcgc
     1141 caagctgttg ccgcgcttgt cggacgcaaa ggtatagact actcctccgc gtacagtatg
     1201 atacaccaac gcttcaacgt cgaatccgtc gaagacctcc ctgccgggaa gctgcccgaa
     1261 gccgtcgcct acgtccacgc attgacactg cacacgggct tgacaggcga agtgttggac
     1321 gcaccgccca aagccgagcc gaaactgccc atcgacggca actctttagc cgacattgcc
     1381 gctatggttt attacggcac atggatgatt gaatcgggca aagacatctc cgcgccgctg
     1441 aagcagctcg gctgcaggca ggcggttacg atgtggacgg tttggcacga aacccgttca
     1501 atcctgaaaa gatccgccgc ggccctcgaa gtgttgcggg gatatgcgga caaagacgcc
     1561 tccgaccgca tagccgcatg tcttgaaggc atttacggca aggctacggt aaggtaaacg
     1621 gaaaatgccg cccgaatctt cggacggcat ttttcacgcg gcatagcgtt tcaacatttc
     1681 ccgataatcc gccggttccc gtccggcgaa ctcaaaggcc ttcaaatcgg cgttatctga
     1741 tgccttgcgg ctctcgttca gccaataggc gatctgtttc ctgcaaaact cttcagcact
     1801 catcgtcaaa tccaaaaatg cctgttcagg tgcggcgcat cctcacgcgt gatacggcgc
     1861 aaatcactgt cggccggcag gccgaaatac agctcgaaac gcctccttgc cgattccgaa
     1921 cgctgcgggt cgaaagtctc cgcatccggg atactgaaag cctccgccaa agcccaataa
     1981 accaaattcc gatgatgtat ggaattgatt tccggctcgt ccccgtcccc cgccatatcc
     2041 cgcggcaggc aatacccccc ggcaaacaac ctgccgtcac ggtccggcgc aggcgcaagc
     2101 gtcagcttcc cgtcgccctg caccgcataa accggcagcc ccgtttgcgc cccgtcgcgc
     2161 caacccgggg cgcaaacatc cgccgcctcc ggcgaaacca acttcaggca caccggcttg
     2221 ccgtcggaca caaaacggat actgtcgatt tcatacgcat aacccgccgc aaacccccct
     2281 gccgcataag cataaagcgg ccgccctttt tccacatccg ttacaaacgc atcgtccgaa
     2341 tgcagcagcc ggccgcgcag gcacgcctcg tgaaccgcat cgttcaacca tcccgacacc
     2401 tcttcgtcgc tccaaaaata cggctcggcc ttatcattgg caagcacgcg gaaacgcgca
     2461 atcaaagcct tcaaattcat tgcgcgatac cgaactgacg aaccatccga accacgtccc
     2521 cgcgcaaatt ctccgccgat ttgcgcttat ccaaaccctg gccgtaattc gccttcgcat
     2581 acagttcaag ctcgtccttg cccatccggt tgatttgcag gatcacatcc tgcaccgcgt
     2641 caaacgccgc cgtttcccgt tcgagttccg aagcgcccgc cttttccaaa gcctccgtat
     2701 catccgcctt ttccacgcgt ttgcccggga cggcggcaaa cacatcgccg tggcgcaaca
     2761 actttgccgc agccccggcc gacacctcgc gcacctggcc gtcttcgaaa accaagcccg
     2821 tatgatagat acggtcgcgc cacacggcac gcccgccgat atacttgata aaaaccttat
     2881 ccatccaacc ccccgccgcc gtccggacgg cattgtcaaa tacagcaacg ccgcaaaggc
     2941 aaatccccgg gcggcgtttc attccgctta caactggccg aacttctcgc cgtaaatcaa
     3001 aatatcgata tcggcggcct tcttattttc cgccccttta agcgtaaccg tcagcaaagc
     3061 ctgcttgggc agcgtaacca gtttggcaga ctggcagcgg atacgcccgg cagacgcaaa
     3121 atccgcgccc gaagcaaaat aagccgcatc ctgaggcacg tgcgcatcat ccgcaccgtc
     3181 cgcatacgca aagccgacat cggcagtgat accggaagac atcgccgtct tcacaataat
     3241 ctgcgcgtca tccaaacgga aaccctgttc caagacaccc agcgccaaca catcgccatc
     3301 cttgggcggc ccgtaggcat ccgcccccaa gattgcaccg tcctgcttcg caacgatacg
     3361 gtaatgctcc gccgccagat tgccgtaagg cacgccgccg aaacggttgc cgccattgtt
     3421 tctcacataa atctcagcca taaaaaacct caacccaaca aataggaaaa gccgcccttt
     3481 caagcggcat tcgaatcagc ggataccgcc gtgcaaagga accaccgtat ccacaaccgt
     3541 gacaccgtag tcggtaattt ccttcgcgcc cccgccgaca tccacggcaa agcgcgtttt
     3601 cgccacaccg cgtatcgtac cgacgagcgt ttccacacgg ttgccgtggt caagctcctt
     3661 ctcagaccaa aagaaaggca taccgccgtg tttgccggta ttcgcaaacg cctccaacac
     3721 cgcctggccg cccaaaataa ccgaacggtc gaccgcaaac ttgtccgcaa acgaagccgg
     3781 gattttcaag cccgattccg cctcgctgaa cttatcggcg caatacttca tctcatcgcc
     3841 cgcatagaaa cggatggggc gcggcatttt caccaggata aagccgttcc acaaacccgc
     3901 atcgcccaaa aacagcggat tttgatttgc ctgggaggcg cgcgcgattg ccgaagcctg
     3961 aagctggcgg aatttcggat cggcggcaaa acggttgtac tgcgccgggg aaagcagcca
     4021 cacgcgcaaa ggcgaatcac ccgccgcctt gtcgccctca aacttcacaa tcggcggcgg
     4081 cagcggaatc tggtcgagca cggtacgcat actgtccacc gtatccatcg taaacaaatc
     4141 ggtagaggca atcttcaact cgccgccgtc ctgcccaacg cccgttaccg catcgcccga
     4201 agccgtaaaa tggcggtttt tggacggggc tttgaccgga ttgaccgcat aatcattgaa
     4261 tttcgggtcg cccgccaaag gcacgcccca ttcgatattg tcatgaaaac cgcgcgcgcc
     4321 cgccatatgc accaacagcg tctggtcggc ataacggtcc atcaggcttt gcgccgccgg
     4381 acgaatcagc gcgcgataat cggcagggct gcgctgattc gtcatcgtac cgccgccgtc
     4441 aacggggaaa cgcgcctgat tcacacgcaa acccgcctcc gacaggctca tccccacgcc
     4501 cctgccttcc gccgtgttgt cgcccataat cggcagggcg gaaacagggt tgaccaaatt
     4561 gaaacggatt tcgtcaccca tgccgcgcgt caaatcctga cagcgcacga ccggcatatg
     4621 ctgggtcgtc tgtttgcgca aagtcgcctc cgcacccgcg gtaccggcag gcatcttgcc
     4681 cgccaaacgg ttcagcgtac tgttgcgctg catatgcatc gcaaacagcc ccgccgcctg
     4741 cctcatcatc gcctgcgggt cgccgtaggc cgtattcgtc ttttgtgcca taaagagcct
     4801 ccctcaaaaa ctcaagaatt taaaaacttc tcaacctgct cttccgtcat ggaagccagt
     4861 ttttccgcca gcgcgttgcc gctcaaataa tccggcgggc cgtccgaagc cgtatggtcg
     4921 cgccccgccg gaatatccga cagcgtattg ggcgtattct tcccggcagg cggcttttcg
     4981 gcgggctttt ccggttcggc ggcggatttt gccgccttaa acatatcgaa cgccccgatg
     5041 acctcggcgg cagtcccgtc cttaagcgcg tcattaaacg ccttgcggac aagcgggttc
     5101 tgcccggcca gccactcctc cagctcgcgg ctttccacaa tcgaatccgc atcggggtgc
     5161 gccgtataga tttcgcggta atgcgccttt gccgcctcct gccgcttcat gtccgcctca
     5221 taacccgcca gcttctcctg aatcagcttt tccacgccct ttttcacatc ctcgtcggaa
     5281 aaatccccga acaacgaatt atcggaaccg tcagccgttt ccactccggg tttttccgcc
     5341 cccttctcaa gctgggcaat gcgctccctc aaggcttggg cttcggcttt cgccgacttt
     5401 tccgcctcgc gcgcctctac cagcttttca taagggatag tgtgtttgcc gtccttggca
     5461 cggatgacct tgtccgcacg ccgtccgccg cgccgatacc cgcgccgacc accgcgcccg
     5521 tactgccgac caccagcgat tccgcccact tcttaccctc caaaccgtct tggatatcgg
     5581 ggaaaaccaa ctggtccaaa cccaaacgcc ccgacacgtc cagcggcgtc aggcggctga
     5641 agcccttggc caacacctcg cccgccttat ccccgaaagt gtccgccaac atattgcgca
     5701 acgccgcttc cgcatcccac gggtcatcgt cgtcactgcc caacatcgaa gccaccgcaa
     5761 gcagcgtcga cacaaacggc agccccaaga tgcccgaagc catcgcatgc gagaccaaca
     5821 gccccgccag cgtcttacgc gcctccttat cccccttaaa cgcaaggtac gcattgcgcc
     5881 ccagcgcata caggatattc tgcgaatact gcttgaacag gaagaccacc ttcgccgcat
     5941 tgcccatcat aaagcgcgga cggttttgcg ccgcatagtc aaaatgcccg tcgtacgtcg
     6001 cctttttcgc ctgttcgaaa gccgcctcac tgtccgcccc cgcccgtttc gccaaacggt
     6061 aggccgcgac aaacgtaacc tggcggttaa acttctccgc atgatggaac agccaggccg
     6121 ccttatccat caccttctgc caagccgacc ccgccaaccc cgggtcgccg ttggccaccc
     6181 ccgccaaatc atgcgcctgc gacaaatcga tcacaccgta atccgccgcc tttcggaacg
     6241 ccgccttctc atccccgttc aaactgtcct caatcgtatt gaacttctcc ccgaacctca
     6301 gcccgatctg ttttgaagcc cgcagcaatt cccgcgccgc accggcataa ccccacttcg
     6361 ccgccatcac cggataagcc accattgccg tctgcgacag gttcacaacc gcagaagcgg
     6421 gcgacatccc catcatccac aaaaagccga agcccgtcaa agcctgcgcc agcttggaag
     6481 gattcggatt catcaccgcc tcgtggcgtt tcctcatctc atccgccaca cgctgcaact
     6541 gacgctggtc gaaaccctcc tcatatttgc gcccgtccac aaaatcctgc atcacatcca
     6601 actgttccgc catacggtcc gcatagcgca acttcgccag ataattcgca ccgctgccca
     6661 tattctgcgc atatgcgcgc ctcgcatcat cgctgaagcc cggcacgccc ttgcggtgga
     6721 tgccgtgttt cgcccaagac gtatcgggca gggagttcaa ataaagctgc gtcagcgtat
     6781 cgttcaatcg ggcccgttgc gccggatcca aatccatcct gccgacagcc tcgcccagct
     6841 ccttcataaa tccgctgccg accgcatcgc ggcttcggat ataatcccgg gacttcatca
     6901 cgggcgaaac cttaaacccc ggcccgaaat cagccttcag cgcatcgcgc agcttctccg
     6961 cctcgctcaa agtttccgcg cgggacacat tcgcactatt cccgtccgca tccttgacca
     7021 ccaccagata atcgccgaaa cgcgccaaag ggaaatacac accgcccagc tcgttgtcaa
     7081 aacgctcctt caggcggcgc accgtttccg catcctgccc cgcacgcgcc aaccgttcgg
     7141 caagcgcatc gcgcacctgc ccgaaatgcg ccctataatc atcccgcgcc ttgcggtaca
     7201 ccctctgcgc cgcatcaggc agcccggcaa aacggcggtt cagcaaagca agcccctcca
     7261 aagccttttg cgccccaacc tcatcctttt ccaaacgctt cacatccgcg cgcgcctgct
     7321 ccaacatccg taccgcatcc gttttagccc ggctttccgc cgccgcatcc gccccggcgc
     7381 gcctcaacgc ccgcttcgcc tccgaatccg cataaaacag acggcgcagg cgcatatccg
     7441 cctcatccgc caaaatttcc cgaccgtgtt tttcctgcgc cttctccagc gcataagccg
     7501 ccttatccgc cgcccgttgc gccttattgt aagcagcatc cgcacgcgcg gtacgcgcac
     7561 cggcggaagc aacggccgcc cgcgccttcg cgattttacc gtccgcaata tccaaagccg
     7621 tccggatgcc gtccaaacgc ccccgggcat cctttcgcat cagcgggtcg gcatccacct
     7681 tcgccagcgt cgcatcgtgc atcagatccg caagcgcttt cgcatcctcc tccttcaacc
     7741 tgccccattc cctgacaacc ttgtccgcct ccgtaaccgc cttgttcgca tccgcgccga
     7801 agagttccga caaacgcccg tattccttca ggccgtccaa aacgccgccg taaatgtcct
     7861 cgatctgccg cctgcccaaa aactggagct gaatcccctt ggaagcatcc cagcggtctt
     7921 tcagcgcgtt tctcaaatcg gattttttac ccgttatacg gctgattgcg gccccgatgt
     7981 tcgccgcacg ggaaaaacgc accgcctccg tgtcaaaatc ggcaatgccg cccttgcccg
     8041 catctttgtc ttgcaaaatc tgcaacaggc gtttatcatc aatgcccgat aaaggcgatt
     8101 tgctttcaga ggctcccgta gttgatacgc ggtcaagtaa tgaaagcaag ttgccttttt
     8161 ctattttcgt cagcttatgg tcgtaatagc ggttaccgtt cctgctaacc gcgacggcag
     8221 cccttaccgt gtaatccgca ccgccgacat tcagtcccga aacataatat tcataaccct
     8281 gaatatcgcc gttcttagcc aaatcctcgt tcggcagcgt atcgatatac accgcattct
     8341 caataatctg cggaattgcc gcgatactct gcaaatgttc cgcgtcctta tagtcgtgac
     8401 gcaatatttc agtgatgccc gaacgtccca aactgatttc acgccccgtg tccttattca
     8461 cataaggccc gcgcaaagat ttgccgtatt ccagcgcatt gcgcttatat tcgcgcaaat
     8521 tgccgccttc cggaacctcc ctgcccgaga tacggatggt ttccgcacgg cgcagcttct
     8581 caaggctttc cgacttggaa cgttccgccg caccgtcttc agcccgcgcg aacatcactg
     8641 cacgatgatt gccgtcccaa ggcgcatccc gcgccccttc cacattcccg tgcaaatccg
     8701 ccagcatcgc aaacacatcc gcgtccgaca tcgcaccgcc ggccttgccc gccaggcgtt
     8761 gcagcacacg gcgcaacagg gcgccgatac gcgccaacac gcccgcaatg ccgcgtttca
     8821 aaccgttgcc gaccttcacg ccgtaacggt tttcaatgcc cgcccaaccg ccggtacgct
     8881 gcgccgcata aagctccgcg accgcctctt ccaccgcggc ggagcgcacc gaggccgccg
     8941 catcgcccgt gccttcgcgc ccttcctgca ccgcgtccgc aatgcgccgt atcaggccgt
     9001 tgccgtccgc acgttccaat tcttcacggt acttggcgaa accatccgcc gcaaagcccc
     9061 tgtgccccag ctcgtgccac gccgcccata ccgcgcgttc gggcgtaagg ttttccgcca
     9121 ccagcgtgat tttgcccgtc ctgccgtcaa accagccctc cacacgctgc gacaacagca
     9181 tctgcgcctt gtccggcgcc gtttcggaca cggcggcgac atccacccgt tccgccagtc
     9241 cgcctatgct ttcggcgagg cgttttttga tattttcggc gtgctcgcgc aatgccttaa
     9301 aaccttgcgg cgcctgacgc tctttcaccg tataaacagg ccctccctgc aacacacgcg
     9361 ccttgcgccg tgcctcttcc ggattgtccg aagccgtttt cgcaaacgtt tcaggcttgg
     9421 cagccttatc cgaagacggc ttttcggttg ccgcgccttg tgcggcggcc gacttgccct
     9481 ttccgccatc gaagtgggat tttcccgatt cgatatttcg caccttatcc gaagacggct
     9541 tttcggttgc cgcatttttt gcagcagccg ccttgcccgc aacaggcgta tcttttgccc
     9601 gggcaggctg cgccctgccc gccggtttgc cgtcggcggc ggcatcgcgc ggccgcgcca
     9661 tcaccctggc cggcgccctt gcttcctgcg tcggacgata atcccggccc ggctcggacg
     9721 gcacggcttc cgacaaaccg cccgcccgca aggtttccgc ctcccgcccc ttcaaataac
     9781 ggcggccgtc ggagacaagg ccgtcagagt tggaaggacc ggccatatcg gcaaactcct
     9841 ccggcatccc gcctttgccc ggcctgcccg gcacgcccgc aagcgcatca atatgcctgc
     9901 ggtccgtatg ctcgaaacgg tattcgccgc cccccaaagg cacacccgcc ttttccggcc
     9961 tgcgcctgtc cgccgccgca tccgccatcc gtttcttagg cgataccctg cccgccgaag
    10021 ccgcaacctg cggattcaat accggcaaca ccgtatcggg gcgtttgccc gcaacaggcg
    10081 cacgtttccc gccgtccgtc ccggcaaggt tttcagacgc ggacaggccg ggcgcggcac
    10141 cttccgcgcg cgcccgaacc tgtaagtttt gccgacgggc cggcgcattg ccggacgcaa
    10201 cggcggcgga ccctggcaaa ccctgcaagg cagccgcatt tgcatccgcc cccgcatcag
    10261 gggtcaaacc cctgccgcct tgggtcaaac cctgccgcac atcctgccgc acatcctgcg
    10321 ccaaaccctt atcttccgtg ggtaaagccc gggtcaaacc cctgccgccg ccgacaacgg
    10381 cctcagacgc gccgtcatcc ggcaacgccg tccgaacccc ctgcaaacgc tccccctcgc
    10441 ggctcaacag cggcacgctc caaccgttgt tgcggcggta accgtaaacc gcacggttaa
    10501 gcctgtccaa cacatccgcc gattccggat gttccgcacg gtaagcgcgt acagccccgg
    10561 ccgattccgc caaaccctca ggataatgtc cgcgcatatc cgccataccc gcctcccggc
    10621 gcataatccc gtccgccgta tagtccgcca ccgtctgcgc atgacggcgc accccttccg
    10681 gcagcgcgtc aaaccgcctg cgcgattcct tatccaacgc atcggcaggg acggaaaacc
    10741 cttttttagg aacaggcacg gcatccgcca tacgcacggg cggcggcagg taaccctgtt
    10801 tggcttcgga agccgcctga taatccgcct caatccgcgc gtgatacgcc tgctcgattt
    10861 ttcggcgcac aggcgcggcg atttgcccga aatccgcaac acccttaata tcaaggcccg
    10921 cctccttcgc caaagccttg cgcccgcgcg tatccaagcc gtcgaaatat tggcccgcag
    10981 gcagcggcga gacccgcgca atcccgcccg aagcagcggc ggcagaaccg cccggcgcaa
    11041 ccgaagccgc accgcgcggc gcaccgccgg ggcgcacatt gccgtaagcg gacggaacac
    11101 cgcccctgtc cgcatccgcc gaccgtccgg acggcgcatc atccgcaacc gaccgggctt
    11161 ccatatcgtc agacaccgca gacggcacag ccgcgcccga atccgccgcc aaagcagcgg
    11221 ccgcagaaac cgcaccgtca tccggatcaa tccccaccgc ctccgacggc ttggcccggt
    11281 atttagccgc catgtccgca tactgacgcg ccaaaccgtc cggcatcacg cccgtatcca
    11341 aaaattccgc cccgtcctcc gacgggatac ccgaaacatc cggctgtttt tccgtcccgc
    11401 gcgccaattc ctccgcgtgc ggcgtattgc cgtcggcagg gccgctgaaa aaccgccccg
    11461 cctcctcctg cttcgtacgg tccgcaaact gccgcaaagc cggatgcgac tgatcgtgat
    11521 gcgccgtatc aaaacccgcg ccgccctgtt ccgcgccgcc gtccgcagcc gcctgtgccg
    11581 ccgcacgcaa cgcctgcgcc ccttccgggg ccggcctgcc cgccttcctg ttgcccccga
    11641 tacccccctg cgcacccgcc ttccgtccgg ccggcggcct gagcgggtca gttaccgccg
    11701 cataagcctt cgttccggca aaccccaccg cagaatgccc caaagaagag aaagcctcca
    11761 aagccgcccc cttctcgtcc cattccccgt ttgccagccc cgtacccaaa tactcgcccg
    11821 caaactcacc ggccgattcc gtcgcataag ccgccgcgtg ccgtgccgca ccgcccaaac
    11881 cgcccttggc agactccttc gccagcgccg caaattcagg cgttccctta gccgccgcaa
    11941 ccgccgcctt atccgccgcc gcaacaccca ttttctccaa tgcggctttc ccggcggcct
    12001 tcttgcccat attcaaaata ctgccgccaa gtttcatcgc cgccatatcc gccgcgccga
    12061 ccaccgcgcc cttgaccgcc gcatttttca acgcacccgg cgcacctcgg ccgataaacg
    12121 ccatcaccgc atccttgtcc gcagggtcga cgcccgccgc ctctgccgcc ctgtccagct
    12181 gcccgccgta ttccatcagc gtattgccca aggtcgcgcc cgcataggcg ccgacagccg
    12241 cgttaccgcc cgccaaagcg cccaatatgc cgccggcggt tgtcgtagca atagggacaa
    12301 gcgcattggg cgcctgctcc agcaccgcgc cccccatctc gcccacatct tccaaggcgc
    12361 cgtccaaacc cttctcgcgc cagtccttct taagctcccc ccacacattc gacaggccgc
    12421 cgccgacacc cccgcccttt tcccaagcct cgcccagctc cctgcgcgct tgggatttgc
    12481 gcccctcctg aatcttccga taatccatat cctcggcctt gagccggccc agttcctctt
    12541 tgtcgcccgt aagggcattc caccccatac gcgccgaacg catagaattt ttaaacccgc
    12601 gcgtcaacgc cgtatcgtcg gcggccgccc gcgcatcctt ttgcgtaaag ccgcggtact
    12661 ccttcaaacc cccggcaagc cgcccgtgtt ccaacggatc gtatctgacc aaatcactca
    12721 ttgctcaacc ctcttctcac ccttctcatt ctcataaatg attttaccgc cggccgtttt
    12781 ctgaccgttc ggcttaaagc ccaactgcgc ataacgcttc tcgcgctctc ccgcaaactc
    12841 cacatccaaa ggcttgcccg tccgcaaatc gacaatcccc tcgcgcttcg ccgtcaagcc
    12901 cgtatccggg tcggcaacct cataactgat agtcttgaac acattcgggt caaagccctt
    12961 ctccccttgc cgctcaaact tcccgttaag cgcatcaatc tgccgcgcaa tctccccgcg
    13021 ctgcccgtcc gacttcgcaa aaccataagc ccgcgtcaaa tcggcgatgc gcttcctatc
    13081 ctcaagctcc agcgcatccg cctcacgctt catctcaaac ccctgccgct tcatccccag
    13141 ctcggcctgc tgcacatcaa acgccctgtc cgcattcctc tgcgccatct cgcggttcaa
    13201 cgcaccctcg ttcgccttaa ccgccgcatc aaaccgcccc tcctgcaaca ccgcctcacg
    13261 gttcgccgca ttctccgccg cagccgccgc cagcctttcc ctgcccaatt ccctgaccgc
    13321 cgcattttta ttatgttccg ccacaatccc gcgcgccgca ttaagctgcg ccgccgtcaa
    13381 ctgaccgttc aaggcccccg catgaggcgt agtcgccctc ctgtaaagct cccgctcctc
    13441 aggcgacagc gcatccggcc cggcagacac caaaggacgc tgcgcgtcaa aatcacgcac
    13501 cggtttgccg tcccgataca cattcccgtc aaatcccaaa ggcggcggcg tccgtccgcc
    13561 cgcatccgtc gggcgcgtat cgcgccgcgc gaaatccgcc ccggcccgcg catcggggga
    13621 aagcgcctta tcgccgcccg aaaaccccaa accggcatcc gcaccccgtt cgaaagcacc
    13681 cgcccggcgc aaccgccgcg ccccatcctc aatattgtta cggctcaaat ccatcacatt
    13741 ccgataacgc gccggcccgg cctcataatt cgcaacggta cgccgcaccg catcggcacg
    13801 gtgcgcctgt gccgccgccc gctcccgctc ctccgccccc ttccgggcct tctctttttt
    13861 ctccttttcc ttcatctccc aaaaatcccc ggcagacggc tcaaacaaac ccatcgcaaa
    13921 ccctccgaac aaataaagcc caaaataagc aaaaacccaa acccaatcaa acgcctaaag
    13981 ttaagaacaa acaaaaatcg caaccacaaa ccaattaata aacaacaagt tacacaaaaa
    14041 aacacaccga cgggccattt ccgggggcaa ataaaaaccg ccttcgtgca aggcggttgc
    14101 aaaaaagcct ttcatttaac gaaaggcttt ttgccgcccg cgtaccgcgt tcagaggttg
    14161 tccgacaccg tcggcgtttc catgcctgtt tgggcgtgat tcgcactctt tggcggcacg
    14221 gccgtcatcg ggccggtatc cgcgccgtag gcttcaggga tgccttcggg cggtatttgc
    14281 gtttcaggcg gcatggcggg gacggggaag ccgggatcgt cgccccgcgc cgccctgatg
    14341 ccttcttcga taccggtcgc gttttgcacg aaaaccgact tgcgccccag tccggtcaaa
    14401 atctccatcg tacttttgcc tgtttggaag ttgcgcgtcc tgccgtcgtg cggcaggaag
    14461 tccgtccccc accggtaggg cagcttttcc aattcggcaa cataccagtc caacgtccgg
    14521 tgcgtgtctt cgatgtagcc gatgatgcgc acgctcgtca aatcgcgctg caccagcccg
    14581 atggtcatgg catcgttcca gcccaaatcc caaaccgtat gcacgggcaa agaagaatca
    14641 taagggacga gtgtaacgcg tccggaatga aaagcgtcct gtatttcatg ccggtaaacc
    14701 gccccctccg ataccatgcg cggcctgcct tcccaaatat tcccgtagtc ctctttattc
    14761 atcgaacgct gtgctttgag ccgctcccgg ttcaatgctt cgggaaacca cggattgtcg
    14821 cgccagttga tttcgcacaa ccaagtgtct tcggacggca tagcgataaa acgccggtag
    14881 gtttcgtccg tctccatatc gggattgagg gtaatccaaa tttccgaacc ttctttgcgt
    14941 atggtcggcg tgagcacgtc ccagcttttt ttactgacgc cgtgcccttc ctcaacccat
    15001 acgatgtcga taccttcaaa cgatttgatg ctgtccacgg tatgcgactg caggcccgaa
    15061 aacacgaaca gcgtgccgtt tgcgccgcgt atctcgaagt cggttatttc gtagaaatgc
    15121 cccaaaccca actgcgccac tttgtctttc aacaggcggt gtacggtatc gcgcatcgat
    15181 ttttgaattt cgcgcacgca taaaatacgc aaagggcgtt gcgcccccag cgccagcagt
    15241 gcggatgccg cgccgtgcga tttgccgccg ccgcgcccgc cgtacataac cttgtaccgg
    15301 caaggcttga acagcccgtc cagttttgcg ggcaatttca aatcaaccgt ctttcccgtc
    15361 atcttcaggc cctacaaatt cgatacggat gcccgccgtc ttggcggatt ccgaaatctt
    15421 cgattccgcg gtatccatac cgtatgattt acgctcgatt tccaccaaaa ccttaaaccc
    15481 cgccgccacc gtttggaaca tacgcgcctt tgtgaatgtg tcctccgggc ttccgtacaa
    15541 ttccgcgaca atctgctccg cgcttttgga caggttttcc gccaaattcc gatgcctgcg
    15601 ctgtacggcg gcaacccgtt tcgcttccgc gtccgttact tgtttaaggt tgaaatccgc
    15661 ataatccggc gcattgccgt ttttgtccgc ggacagcacc atcgcccgcg cctttgcctg
    15721 tacctcgtca ttcaaatcgc gcgtccacac gcgttctttg gcaaccctgc cgacgtgttg
    15781 cgccgaaaca ttgtatttgc ggccgatttc ggcaatgctc aaaaccccgc gccggtaatc
    15841 cagctcgact ttgcgccaat cttcatctga caatctcggc attaaaggtc caacctcccg
    15901 tttttggcgg aacattccag ataattccgc aatactgaaa actcgcgccc catctgaata
    15961 ctggaaaact gctgcccgaa ccccgacatc aaagccccca acgagcgcat ttcctgcggg
    16021 ttgaggtgca ataccgtatc gcctatttcc aatacgccca tccccgaatc caataccgat
    16081 acgctgacgg ggcgcgtctc gccaaaccct atggcgggct tgaaaatccc ccttgtcacg
    16141 cggtagattt tgccgtgctc gaccagcagc ttggtaaatt tagtgatttc cgacattttc
    16201 aggcctgtcg tttccgccaa agcctcgcgg ctgaccacct gctccgcgtt gtgcaaatcc
    16261 acaaccccct ggtacacctt gtcaatatcc gacaatcccg acatcaaatc aaactcctaa
    16321 acatcccaaa cgattccaaa ctccgccgcc gcccgcgatt gcaggcggtt acactacaaa
    16381 gccaaaatct ttagatgctg cctctctcgc tttcactgca tcttcaatat gttcaaataa
    16441 acctaatatt ttattttttc tattaaccat aatcctagct tgccatttct tagacggctt
    16501 atggaaataa accccagtat gcccgctagt attattttta aacttccctc tatttttcat
    16561 attttcagca tgtgtaacat ctctcaaatt agatattcta ttatctgtct tatccctatt
    16621 gatatggtct attgaagaag cgtgtttccc ataaacaaaa agccaaatta atctatgtgc
    16681 attatattgt ttcttaaata tttttaactt caaatacccc aacctgcctt tgtataagaa
    16741 aacctccttg ccggaaaatc cggtattcca agatttgtaa tgcaaaccgc tattgaaata
    16801 ctttcttgga cgctccgccc aatataactt tcccgtattg tcatcatatc ttaataattc
    16861 ttttaaaacc tcttgtgtta atcccataaa acaccatgct ccatagatgc ccaagatata
    16921 atcttttctt gatattcaga catttcccga actgataatt ttgtcgttga tatacccatt
    16981 aattgcccgt taggcatttc aatacatcca ataaattttc tttttaaaaa ttcatgccat
    17041 acatcttgac taaaccttct cccatcgata aaaacttttt ctgaaatttc acgatacaaa
    17101 aaccatagcc gcctgttctg ctcgacgctc cgttttgact tgcgcgggcg gattgtgatt
    17161 tcaaggttgc cgttttcaaa ccacccgtcc gggctgtccc aaatcgaccg cataacctgc
    17221 tttttgtttt ggggcgtcag tgtaaattcc gccccgttca tttcagacgg cctttcacgc
    17281 cgacaacccc caatgcttca aggcggcgta tcgtgcgcaa ttgcgaacgg cgcatataaa
    17341 actctttgtc ttcgcggctt aggcctgcgc ccctgccgtc gatcgcgccg cggcggcaac
    17401 cgcacccgaa acccgcgccc aaaccgccgc ttttcaatcc cgccccgtgc gtctcgcccg
    17461 ggaaacggca caacacgacc gtttcagggt tgtcattgca cacgcccgcg atgttcggcg
    17521 tgcattgccc gcctttggcg gctttgcgta tcgcgctaga catcccaacc ctcctgtccg
    17581 atgaaatccc aaccctcgtc tgccgcatcc gtctgttcca tctccaa
//
LOCUS       DBJGGI010000041        17472 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.041, whole genome shotgun sequence.
ACCESSION   DBJGGI010000041 DBJGGI010000000
VERSION     DBJGGI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17472
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.041"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..541
                     /locus_tag="ACSIGJ_001360"
     CDS             <1..541
                     /locus_tag="ACSIGJ_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HHQ1330206.1"
                     /translation="AALLAAALCLTGIFSPLTSLLLPENYAAVRFTVVSCMLPPLFCT
                     LAEISGIGLNVVRKTRPIAFATLGALAANLLLLGLSVPAGGARGAAVACAASFWLFFV
                     FKTESSCRLWQPLKRLPLYMHTLFCLASSAAYTCFGTPANYPLFAGVWAAYLAGCILR
                     HRKNLHKLFHYLKKQGFPL"
     gene            538..1605
                     /locus_tag="ACSIGJ_001361"
     CDS             538..1605
                     /locus_tag="ACSIGJ_001361"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HHQ1330207.1"
                     /translation="MKIILTTSMSGLGGTETATVRLGRLLKRHGHDIILASSDGPFVG
                     EAQASGIRWQPVDFYRGGLAGYLKSTFAYARMLRREQPDIIDCQMARVVPACALAAKI
                     VSPKTKIICHSHGLDAATYPKTAKLFDKLGAYIIGNCKHEREKLIRHGFPAGRIAYAY
                     NTPPPEFHFRKTEKECAVLGTLSRLDTVRAVHLMLDILKKMVGRNIPVRLNMAGIGEE
                     MDNLKAQAKRLGIEDKVTFLGGVRDLTGYFKEVDILVNTPHCVGDHGAGVGNNILEAG
                     LYDTPVVTYNMAGISEMVITGQTGYCIPFGDDEAFIEAVDTLIKHPELRSQMGKALHK
                     HVETLCSDDEIYRTTMAAYEM"
     gene            1619..2791
                     /locus_tag="ACSIGJ_001362"
     CDS             1619..2791
                     /locus_tag="ACSIGJ_001362"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HHQ1330208.1"
                     /translation="MNITIAAPYCSLPSEPHFNRFWYLAELLSQSHDVLLITSNFKHY
                     DKSFRRPEDAEAASQGRLKVILLEESGYSKNVSLGRVTSHHRFVKHFEKWLENCRPGE
                     QDVVYSAYPLIATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHKLLPFA
                     SRANRAYRYADALVAVSQTYLDRAKETNPNVPGEVIYIGADFPKLDAAPAKDFGDDKT
                     RFFYLGTLSYSYDVETVCKGVRKLLDDGENVELHIMGGGPDLDRLKQYACDGIKFYGY
                     IPYAEMMSVAKGCDIAVNAIRSYSMASITNKLSDYMALQKPILNSQVHDEVAEVLTLL
                     PHANYRSGDVDGFVRAAKDILKRKNDPVQSEEIVRRFRRDISYRKIVNLIERLADE"
     gene            2784..4047
                     /locus_tag="ACSIGJ_001363"
                     /pseudo
     CDS             2784..4047
                     /locus_tag="ACSIGJ_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
     gene            4178..5353
                     /locus_tag="ACSIGJ_001364"
     CDS             4178..5353
                     /locus_tag="ACSIGJ_001364"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HHQ1330209.1"
                     /translation="MLNTALSPWPSFTREEADAVSKVLLSNKVNYWTGSECREFEKEF
                     AAFAGTRYAVALSNGTLALDAALKAIGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAVIVVHLAGMPAEMDGIMALAKEHDLWVIEDCAQA
                     HGATYKGKSVGSIGHVGAWSFCQDKIITTGGEGGMVTTNDKTLWEKMWAYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAVIGRIQLKHLPEWTARRQENAAKLAESLR
                     KFKSIRLIEVAGYIGHAQYKFYAFVKPEHLKDDWTRDRIVSELNARNVPCYQGGCSEV
                     YLEKAFDNTPWRPKERLKNAVELGGTALTFLVHPTLTDDEIAFCKKHIEAVLTEAAR"
     gene            5401..7311
                     /locus_tag="ACSIGJ_001365"
     CDS             5401..7311
                     /locus_tag="ACSIGJ_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HHQ1330210.1"
                     /translation="MNLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSDEW
                     FDFANWQSFLLTALLTITLFIRMGLYRAVTRFVSFHVLTTAFAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSDHQKKTHDPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDNPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPSATQEQ
                     RRRIISKLKAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMNAD
                     TEGKTVMVTGAGGSIGSELCRQIIRRRPERLLLFELSEFALYTVEKELCEYCARNGIA
                     AEILPFLGSVQNRTLLTHIMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVETFVLISTDKAVRPANTMGAGKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPITLTHPDITRYFMTIPEAAQLVIQAGAMGRGGDVFVL
                     DMGESVRITDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADETMLPWHELSALLDRIRTACDRYDQQAIRDLLVNAPTGFTPTGGICDL
                     LWVCETHRKNAV"
     gene            7367..8659
                     /locus_tag="ACSIGJ_001366"
     CDS             7367..8659
                     /locus_tag="ACSIGJ_001366"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HHQ1330211.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFPNREYGWNLTVGNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHVEGRHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPPIIDNAYGMPFPNIIDSGVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEAVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKPYDNV"
     gene            complement(8711..9547)
                     /locus_tag="ACSIGJ_001367"
     CDS             complement(8711..9547)
                     /locus_tag="ACSIGJ_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HHQ1330212.1"
                     /translation="MIDLHCHSTVSDGMLPPAEVVRLAHQNGCTLLALTDHDHTGGIS
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLADVRKGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIKNGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLADCVSAVNGAGGMAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLADRFGLLASAGSDFHRLDDFSGGILGACPDLPENCKPVRAHFS
                     RH"
     gene            complement(9842..10045)
                     /locus_tag="ACSIGJ_001368"
     CDS             complement(9842..10045)
                     /locus_tag="ACSIGJ_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HHQ1330213.1"
                     /translation="MDTTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRKKLKEAGWPDDAV"
     gene            complement(10023..10256)
                     /locus_tag="ACSIGJ_001369"
     CDS             complement(10023..10256)
                     /locus_tag="ACSIGJ_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HHQ1330214.1"
                     /translation="MEATVYLEDNEYIALCDLLKLAGLAESGGQAKAFIAEGLVLRNG
                     GTEIRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            complement(10268..10504)
                     /locus_tag="ACSIGJ_001370"
     CDS             complement(10268..10504)
                     /locus_tag="ACSIGJ_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330215.1"
                     /translation="MPIAGVSGSPRLRMCISVNADSSGNRICSLPLGRISLDGWRHTG
                     FQTAFLRNRQCGHIAGFAAILGAAAAGATITPSF"
     gene            10789..14223
                     /gene="dnaE"
                     /locus_tag="ACSIGJ_001371"
     CDS             10789..14223
                     /gene="dnaE"
                     /locus_tag="ACSIGJ_001371"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HHQ1330216.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRGAGIKPVGAADVWIGNPNAPDKPFRAMLVIRNDAGYLRLSELLT
                     EAYVGQDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDTFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMNRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPSQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDCLIKLSNEGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAQAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLDDAMKAQPQIQELLEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTAGDIVDVGKIPLDDQTAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPNMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLGR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDSSGQAEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRIMANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            14289..14519
                     /locus_tag="ACSIGJ_001372"
                     /pseudo
     CDS             14289..14519
                     /locus_tag="ACSIGJ_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14875..15805)
                     /locus_tag="ACSIGJ_001373"
                     /pseudo
     CDS             complement(14875..15805)
                     /locus_tag="ACSIGJ_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225663.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(15875..16418)
                     /locus_tag="ACSIGJ_001374"
                     /pseudo
     CDS             complement(15875..16418)
                     /locus_tag="ACSIGJ_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
     gene            complement(16364..16579)
                     /locus_tag="ACSIGJ_001375"
     CDS             complement(16364..16579)
                     /locus_tag="ACSIGJ_001375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330217.1"
                     /translation="MSVFRINMTAATVLAALSSPVFAAQTADLETVHIKGQRSYNAIA
                     TEKNGDYSSLKAAIWMTASIPASPPAA"
     gene            16739..17398
                     /locus_tag="ACSIGJ_001376"
     CDS             16739..17398
                     /locus_tag="ACSIGJ_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HHQ1330218.1"
                     /translation="MTPPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRALLEMPSEAAVADIARTYSAHYLNPNNRNMTLFPDAPPCLDKLKAQ
                     GFRLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGLCGELGLDPK
                     EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLGAPHLAVLDGLSELPGFLARHY
                     A"
BASE COUNT         4002 a         5259 c         4582 g         3629 t
ORIGIN      
        1 cgccgccctg cttgccgccg ccctctgcct gaccggcatt ttctcgcccc tcacctccct
       61 cctactgccg gaaaactacg ccgccgtccg gtttaccgtc gtatcgtgta tgctgccgcc
      121 gctgttttgc acgctggcag aaatcagcgg catcggtttg aacgtcgtcc gcaaaacgcg
      181 tccgattgca ttcgccacct tgggcgcgct ggcggcaaac ctgctgctgc tggggctttc
      241 cgtaccggcc ggcggggcgc ggggcgcggc ggttgcctgt gccgcttcat tctggctgtt
      301 ttttgttttc aagaccgaaa gctcctgccg cctgtggcag ccgctcaaac gcctgccgct
      361 ttatatgcac acattgttct gcctggcctc ctcggcggct tacacctgct tcggcacgcc
      421 ggcaaactac cccctgtttg ccggcgtatg ggcggcatat ctggcaggct gcatcctgcg
      481 ccaccggaaa aatttgcaca aactgtttca ttatttgaaa aaacaaggtt tcccattatg
      541 aaaatcatcc tgactacttc tatgtcgggt ctgggcggca cagaaaccgc caccgtccgc
      601 ctcgggcggc tgctcaaacg gcacgggcac gacatcatcc tcgcctcctc ggacggccct
      661 tttgtcggag aggcgcaagc atcgggcatc cgttggcagc cggttgattt ttaccggggc
      721 gggcttgccg gctaccttaa aagcaccttt gcctacgccc ggatgctgcg ccgcgaacaa
      781 cccgacatca tcgactgcca aatggcgcgc gtcgtcccgg cctgcgccct tgccgccaaa
      841 atcgtttcac cgaaaaccaa aatcatctgc cactcgcacg gtttggacgc ggcaacctac
      901 cccaaaaccg ccaaactctt cgacaagctg ggcgcgtaca tcatcggcaa ctgcaaacac
      961 gaacgcgaaa agctcatccg ccacggcttc cccgccggcc ggattgccta cgcctacaac
     1021 accccccccc cggaatttca tttccggaaa accgaaaaag aatgcgccgt actcggcaca
     1081 ctttcccgtt tggacaccgt ccgcgccgtg catctgatgt tggatatttt gaagaaaatg
     1141 gttggccgca atatacccgt acgcctgaac atggcgggca taggagaaga aatggacaac
     1201 ctcaaagccc aagccaaacg tttgggtatt gaagacaaag tgaccttcct cggcggcgtc
     1261 cgcgatttga ccggctactt caaagaagtc gatattttag tgaacacgcc gcattgcgta
     1321 ggtgaccacg gcgcaggtgt cggcaacaat attttggaag ccggccttta cgacacgcct
     1381 gtcgtgacct acaacatggc gggcatttcc gaaatggtca ttaccggcca aaccggctac
     1441 tgcattcctt tcggcgatga cgaagcgttt atcgaagccg tcgatacact catcaagcat
     1501 ccagagttgc gcagccaaat gggcaaagcc ctgcacaaac atgtcgaaac cttatgctcc
     1561 gacgacgaaa tctaccgcac caccatggct gcgtacgaaa tgtaaggccg tctgaaacat
     1621 gaacattacc atagccgccc cctactgctc gctgccgtcc gaaccgcatt tcaaccgctt
     1681 ttggtatctg gcagaactat tgtcgcaatc gcacgacgtg ttgctgatta ccagcaactt
     1741 caagcactac gacaaatctt tcagacggcc cgaagatgcc gaggccgcct cacaaggccg
     1801 tctgaaagtc atactgttgg aagaaagcgg atacagcaaa aacgtatctt tgggacgcgt
     1861 gaccagccat caccgcttcg tcaaacattt tgaaaaatgg ttggaaaact gccgtccggg
     1921 cgaacaagac gtcgtctatt ccgcctatcc gctgattgcc accaacctgc tgttgggcaa
     1981 acacaaagcg cgtttgggtt acaaactgat tgtcgatgtg caggacgtat ggccggagtc
     2041 tttctcctcg gtcgtaccgt ttttgaaaaa agtaccgcac aagctgctgc cctttgcttc
     2101 acgcgccaac cgggcctacc gctacgccga cgcgctggtt gccgtatcgc agacttatct
     2161 cgaccgcgcc aaagaaacca atccgaacgt acccggcgaa gtcatctata ttggtgctga
     2221 ctttcccaaa ctcgatgccg cacctgccaa agattttggc gacgacaaaa cccgcttttt
     2281 ctacttgggc acgctcagtt acagctatga cgtggaaacc gtgtgcaaag gcgttcggaa
     2341 acttttggac gacggcgaaa atgtagagct gcacatcatg ggcggcggcc ccgatttgga
     2401 caggctcaaa caatatgcct gcgacggcat caagttttac ggctacattc cttacgccga
     2461 aatgatgtcg gtcgccaagg gctgcgacat tgccgtcaac gccatccggt cctattcaat
     2521 ggcatcgatt accaacaaac tctccgacta tatggctttg caaaaaccga ttctgaacag
     2581 ccaggtccac gacgaagttg ccgaagtcct taccctgctg ccgcatgcga actaccgttc
     2641 cggcgatgtg gacggtttcg ttcgagccgc caaagatatt ttgaagcgca aaaacgatcc
     2701 tgttcagtcc gaagaaatcg tccgccgctt caggcgcgac atttcctatc ggaaaatcgt
     2761 caacctgatt gaaagattag ctgatgaata aatttttcaa acgcctcatc gacattgtcg
     2821 cctccgcatc ggggctgatt gccctgtcgc ccgtgttttt ggttttaata tacctcatcc
     2881 gcaaaaactt aggttcgccc gtcttcttca ttcgggaacg ccccggaaag gacggaaaac
     2941 cttttaaaat ggtcaaattc cgttccatgc gcgacgcgct tgattcagac ggcattccgc
     3001 tgcccgacag cgagcggctc acgcccttcg gcaaaaaact gcgcaccgcc agtttggacg
     3061 aactgcctga actgtttgga cgaactgcct gaactgtgga acgtcctcaa aggcgagatg
     3121 agcctggtcg gcccccgccc gcttttgatg cagtatctgc cgctttacaa caaatttcaa
     3181 aaccgccgcc acgaaatgaa accgggcatt accggctggg cgcaggtcaa cgggcgcaac
     3241 gcgctttcgt gggacgaaaa gttctcctgc gatgtttggt acaccgacaa tttcagcttt
     3301 tggctggata tgaaaatcct gtttctgaca gtcaaaaaag tcttgattaa agaaggcatt
     3361 tcggcgcaag gggaagccac catgccccct ttcgcgggga atcgcaaact cgccgttatc
     3421 ggcgcgggcg gacacggcaa agtcgttgcc gagcttgccg ccgcactcgg cacatacggc
     3481 gaaatcgttt ttctggacga ccgcacccaa ggcagcgtca acggcttccc cgtcatcggc
     3541 acgacgctgc tgcttgaaaa cagtttatcg cccgaacaat tcgacatcac cgtcgccgtc
     3601 ggcaacaacc gcatccgccg ccaaatcacc gaaaaagccg ccgcgctcgg cttcaaactg
     3661 cccgttctga ttcatcccga cgcgaccgtc tcgccttctg caataatcgg acaaggcagc
     3721 gtcgtaatgg cgaaagccgt cgtacaggcc ggcagcgtat tgaaagacgg cgtgattgtg
     3781 aacactgccg ccaccgtcga tcacgactgc ctgcttgacg ctttcgtcca catcagcccg
     3841 ggcgcgcacc tgtcaggcaa cacgcgtatc ggcgaagaaa gccggatagg cacgggcgcg
     3901 tgcagccgcc agcagacaac cgtcggcagc ggggttaccg ccggtgcagg ggcggttatc
     3961 gtatgcgaca tcccggacgg catgaccgtc gcgggcaacc cggcaaagcc ccttacgggc
     4021 aaaaacccca agaccgggac ggcataagcg attaaaataa acttccgtac aaaccgattt
     4081 tgacaacacc tgcggcgcgc gcccgattct tcggaacacg cccccttcag acggcatagg
     4141 gtcggaaatg ccgtccgaac ccgacggacc aaacaccatg ctgaacaccg ccctttcccc
     4201 atggccgagc ttcacccgag aagaagccga tgccgtttcc aaagtcctgc tgtccaacaa
     4261 agtcaactac tggacgggca gcgaatgccg cgaatttgaa aaagaatttg ccgcctttgc
     4321 cggcacgcgg tacgccgtcg ccctttccaa cggcacgctt gcgctcgatg ccgcactcaa
     4381 agcaatcggc ataggcgcgg gcgacgatgt gattgttacc tcgcgcacct tcctcgcctc
     4441 cgcctcctgc attgtgaacg cgggcgcaaa ccccgtgttt gccgacgtgg atttgaacag
     4501 ccaaaacatc agcgcggaaa ccgtcaaagc cgtgctgacc ccgaatacca aagccgtcat
     4561 tgtcgtccac cttgccggta tgcctgccga aatggacggc atcatggctt tggcaaaaga
     4621 acatgatttg tgggtgattg aagactgcgc ccaagcgcac ggcgcgacct acaaaggcaa
     4681 atccgtcggc tctatcgggc acgtcggcgc gtggtcgttc tgtcaagaca aaatcatcac
     4741 caccggcggc gagggcggta tggttacgac caacgacaaa accctgtggg aaaaaatgtg
     4801 ggcgtacaaa gaccacggca aaagctacga tgccgtgtac caccgcgaac acgcgcccgg
     4861 tttccgctgg ctgcacgaaa gtttcggcac caactggcgc atgatggaaa tgcaggctgt
     4921 catcggccgc atccagctca aacacctgcc cgaatggacg gcgcgccgcc aagaaaacgc
     4981 cgccaagctg gcagaaagtt tgcgcaaatt caaaagcatc cgcctgattg aagtcgccgg
     5041 ctacatcgga cacgcgcaat acaagttcta cgccttcgtc aaacccgaac acctgaaaga
     5101 cgactggacg cgcgaccgca tcgtcagcga actgaacgcg cgcaacgtcc cctgctatca
     5161 aggcggctgc tccgaagtgt atttggaaaa agccttcgac aacacgccgt ggcggcccaa
     5221 agagcggctg aaaaacgccg tcgaactggg cggcaccgcc ctgacgttct tggtgcaccc
     5281 gacgctgacc gacgacgaaa tcgcgttctg caaaaaacac atcgaagccg tcctgaccga
     5341 agccgcacga taacccttca gacggcatat gccgcctgaa agcacatacc gccccacgat
     5401 atgaatctgg aaaccctgat cgccctgccg cgcaacatca agaaaatctg tttcctcata
     5461 cacgattttc tgatgatttt cattgccttt tggttcaccc aaagcctgaa ggccgactac
     5521 tcggacgaat ggttcgattt tgccaactgg cagtcttttt tgctgactgc cttgctgacc
     5581 atcacattat ttatccgaat ggggctttac cgcgccgtta cacgctttgt cagtttccac
     5641 gtcctgacca ccgcctttgc cggcagcctc gcctccgccg tattgttctt cctcaacaca
     5701 ttgatttttg aagaaaggct gcgcctcgcc ctgccgattg tctatttctt actgctgttt
     5761 gtttccgtga ccggctcgcg tatggttttg cgcggactcc tgtccgacca ccagaaaaaa
     5821 acacatgacc ccgtcatcat ttacggcgcg gggcgttcgg gtcggcagct ccttgaagcc
     5881 gtcaaacaga tgcgcgaata ttccgccgcc gcctttgtgg acgacaaccc caagctttgg
     5941 cacaccgtca tctacgacct tgccgtttac cagcccgatg ccatcgcctt cctcatcgaa
     6001 cgctacggcg tggaaaaaat cctgctcgcc attcccagcg cgactcagga acaacgccgc
     6061 cgaatcatca gcaaactgaa agcctatccg tgcgaagtgt tgaccattcc cggaatgaaa
     6121 gacctgatgg acgggaaaat cagcatcggc acgctcaaaa aaatctctgt gtccgacctg
     6181 ctcgggcgcg attccgtcgc acccgacgac cgcctcatga atgccgacac cgaaggcaaa
     6241 accgtcatgg taaccggcgc gggcggctcc atcggttcgg aactctgccg ccagattatc
     6301 cgccgccgcc ccgaaaggct gctgctgttc gagctgtccg aattcgccct gtacaccgtt
     6361 gaaaaagaat tgtgcgaata ctgcgcccga aacggcatcg ccgccgaaat ccttcccttt
     6421 ttgggttcgg tgcaaaaccg cacgctgctc acgcacatta tgaccgcctt ttccgttgcg
     6481 accgtctatc acgccgccgc ctacaaacac gtccctatgg tcgagttcaa caccgtcgag
     6541 ggcatacgca acaacatctt cggcacactc gaatgcgcgc ttgccgccac ggcttcgggc
     6601 gtggaaacct tcgtcctcat ctccaccgac aaagccgtcc gccccgccaa cacgatgggt
     6661 gcgggcaaac gcatggcgga actctgcctt caggcactcg ccgccgaacc cggacaaaaa
     6721 acccgcttca gcatggtacg tttcggcaat gttttaggct cgtccggctc cgtcgtcccg
     6781 ctgtttgaaa aacagattgc cgaaggcggc cccatcaccc tgacccaccc cgacatcacg
     6841 cgctatttca tgaccatacc cgaagcggcg caactggtta tccaagccgg cgcgatgggc
     6901 agaggcggcg acgtgttcgt cctcgatatg ggcgaatccg tcagaatcac cgaccttgcc
     6961 cgccaaatga ttaccttaag cggactcaaa cccaaaacgc ccgaacagcc cgacggcgac
     7021 atcgaaatcc tcattaccgg gctgcgcccc ggcgaaaaac tctacgaaga gttgctcatc
     7081 ggcgacaacg tccgcaaaac cggccatccg cgcatcatga ccgccgacga gaccatgctg
     7141 ccgtggcacg aactctccgc cctgctcgac cgcatccgca ccgcctgcga ccgttacgac
     7201 cagcaggcaa tccgcgacct gctcgtcaac gccccgaccg gctttacccc gaccggcggc
     7261 atctgcgacc tgctttgggt atgcgaaaca cacagaaaaa atgccgtctg aaccttcaga
     7321 cggcataccg tacaaaccaa tctgccttac acacactgag tttgacatgc agttctcggc
     7381 attcggcgaa aaattcacgc aacacagcgg catcctgcaa ctgatggacg acctcggcga
     7441 cgcgctcaaa agcgacaagc ccgtcaatat gctcggcggc ggcaacccgg cgcgcattcc
     7501 cgaaatcgat cgggcgttcg ccgacatatt ttccaagctg gcggcggaac acgccgtcga
     7561 gaacatcggc aactactcca atccccaagg cgatgccgcg ctgattgacg cgctgaccgc
     7621 cttccccaac cgcgaatacg gctggaacct gaccgtcggc aatatcgcgc tgaccaacgg
     7681 ttcgcaaaac gcgtttttct atttattcaa cctcttcggc ggcaaattca agctttcaga
     7741 cggcacatcc gcagaaaaag ccattttgtt gccgctcgcg cccgaataca tcggctatgc
     7801 cgacgtgcat gtcgaagggc ggcacttcgt ttccgtcaaa cccaaaatcg aaaacgtcga
     7861 acacgaaggc gaagcaggct tcttcaaata ccgcgtggac tttgacgcac tggaaaacct
     7921 gcccgaactc aaagcgggca aaatcggcgc gatttgctgt tcgcgcccga ccaacccgac
     7981 cggcaacgtg ttgaccgacg gcgaaatggc gcgtttggac gctttggcgc gtgaacacgg
     8041 cattccgccg attatcgaca acgcctacgg aatgccgttc cccaacatta ttgacagcgg
     8101 cgtaacgctg aattggcacg aaaacatcat cctctgcttc agcctttcca aagtcggcct
     8161 gccgggcgtt cgcaccggca tcatcgtcgc cgcgcccgaa gccgtcaaag ccgtcagcag
     8221 cctgaacgcg attgtgaacc ttgcccccac gcgcttcggc gcggccatcg ccgccccgct
     8281 gctggaaagc ggcgagatga aacggcttgc cgaccaagtc atccggccgt tttaccgcaa
     8341 tcaggcgcaa accgccgttt cgctgctcaa gcgcgagctg ggcgcgtacc cgatgaaaat
     8401 ccacaaaccc gaaggcgcga ttttcctgtg gctctggttt gaaaacctgc ccgtttcgtc
     8461 gcaaaccctg tacgaaatgc tcaaagccga aggtacgctg attattccgg gcgaacattt
     8521 cttcgtcggc atcgacacgc aagactaccc gcacgcgggc gagtgcatcc gtatgagtat
     8581 cgcacaggac gctcaaacgc tggaaaaagg catcgccgcc atcggcaaaa ccgtccgaaa
     8641 accgtacgac aacgtttaaa acgcaaaaaa tgccgtctga agacctttca gacggcattt
     8701 tcatccgcat tcaatgtcgg gaaaaatgcg cccgcaccgg tttgcagttt tccggaagat
     8761 cgggacacgc gccgaggatg ccgccgctga agtcgtctaa acggtggaag tcgctgcccg
     8821 cgcttgccaa caatccgaag cggtctgcca aaagcgcgta gttgaggcgg tcgtttttgc
     8881 agcagttgcc gctgtggact tcgatgcccg cgccgccgag gtttttaaat tcttcaaaca
     8941 ggttgcgctt ggcggttgcc gacaaatcat aacgcatcgg atgggcaatc accgccatgc
     9001 cgcccgcgcc gttgacggcg gagacgcagt ctgccagcgt cgcccattcg tggcgtaccg
     9061 cgcaggattt gccgtcgccc aagtatttgg tgaacgcctg ctgcttgttt ttgacgtgtc
     9121 cgtttttgat gaggaactcg gcgacgtggg tgcggctgac catttctttg tttgccgcca
     9181 gcgccagcgc gccgtcgtat gcgccgccga tgcctttctt ttcgagcttg gcggcgatgg
     9241 cttcaagacg tttcagacgg cctttgcgga catctgccaa caggttttgc aggttttcgt
     9301 cctgctcgtc gaaatccaaa ccgacaacgt gtatggtacg cccgcgccac gttaccgaaa
     9361 tttccacgcc gttaatcagg cgcaaaccga gcctgtcggc ttcggcacgc gcttcggaga
     9421 tgccgcccgt gtggtcgtgg tcggtcaacg ccagcagcgt gcagccgttt tgatgtgcga
     9481 ggcggacgac ttcggcgggg gggagcatac cgtcggaaac ggtggaatgg cagtgcaggt
     9541 ctatcatggg gtgttatgtg gtggtgaatg aaggacggga tgtttcaatg ttgtcggaca
     9601 gtgtggcggc ggcggtcatt ttaaaaaaca ggtgtatggc aaatgcaaag gaaaagccgc
     9661 tatgccgtca ttcccgcgca ggcgagaatc cagactttga tttattggaa atgtttaagg
     9721 ttaaccgcta tttcaaactt ccggattcct gctttcgagg gaatggcgat atgaacgttt
     9781 tcaattttaa tctactataa aagaccgtct gaaaacgtgg ttttatcaag cccaaccgtt
     9841 ttcagacggc atcgtccggc caacccgctt ccttcagttt tttccgttgc gcatcgtagc
     9901 gtgccttgtc cgcccagtaa atcgtctgaa tgcacgcctc gccgcagtcg ctgccgcagc
     9961 attcccacga ctcggggcgg acgggttcgt ccaaaagcgg ctcgcccaaa agggcttcgg
    10021 ctttatgctt cagggtcgta tccatcggcg atttccaagc gcgcgccgtc aaactcgatg
    10081 acttctccgc cgcgtatttt ggccgttttg cggatttcgg ttccgccgtt gcgcaacacc
    10141 agcccttcgg cgataaatgc ttttgcctgt ccgccgcttt cggcaagtcc ggccaatttc
    10201 aagaggtcgc acaaagcgat gtattcgttg tcttcgagat agacggtggc ttccataatg
    10261 tcccctttta gaaagagggc gttattgtag cacctgccgc cgccgcaccc aaaattgccg
    10321 caaaaccggc gatgtgtccg cactgcctgt tccgtaaaaa tgccgtctga aaccccgtat
    10381 gccgccatcc gtccaaagaa atcctgccca acggcaggct gcaaatcctg ttccccgacg
    10441 aatccgcatt gacgctgatg cacatcctca aacgcggact gcccgataca ccggcaatcg
    10501 gcatcaaaac gaaatcaaaa acctgtttga acgtaaaatc tatttaactg cttgccgtcc
    10561 gtccgaataa ggtatatagt tccttataac tagtttgata gtcctttata tctatcaata
    10621 ctccttggga agcctccgcc acacggcagg aggcattttt ttgccgtcgt aaaagctcaa
    10681 aaaacatttg caggtcatgc cgtctgaatc cgaaacggca ttacctgcac cgccatctaa
    10741 agacaaccct gctacaatac gccttttatt gtccacgccg attttgccat gaccgagccg
    10801 acctacatcc ccctgcgcct gcataccgaa ttctccatta ccgacggtat ggtgcggatt
    10861 aaaaaactga ttgccaaagc gcaagaatac ggtttgcctg ctttgggcat cagcgatttg
    10921 atgaacgaat tcggtttggt gaaattctat aaagcctgcc gcggcgcagg gattaagccc
    10981 gtcggcgcgg cggacgtgtg gataggcaat ccgaatgcgc ccgacaagcc gttccgcgcc
    11041 atgctggtta tccgcaacga tgcgggttat ctgcgcttga gcgagctttt gacggaggct
    11101 tatgtcggcc aagaccgcaa tatccatcat gcggaactca atcccgaatg gctggaaaac
    11161 ggcgacaaca gcggcttgat ttgtttgagc ggcgcgcatt acggcgaagt gggcgtgaat
    11221 ctgttgaacg gcaatgaaga cgcggcgcgt gcggcggcgt tgaaatatgc ggcatggttt
    11281 cccgatacgt tttatttaga gctgcaacgc ctgcccgaac gccccgaatg ggaggcttgc
    11341 gtttccggca gcgtgaagct ggcggaggaa ctgggtttgc cggtggtagc aacgcatccg
    11401 acacagttta tgaaccgcga cgatttcaac gcgcacgagg cgcgggtgtg catcgcgggc
    11461 ggctgggtgc tgacggacaa gaaacgtccg cgcgatttca cgccgagcca gtttttcatt
    11521 ccgccggaaa cgatgctgga gcgtttcgcc gacttgcccg aagccttgga aaacacggtg
    11581 gaaatcgcca aacgctgcaa cttgcacatc acattgggca aaaacttcct accccttttc
    11641 cccacgcccg acggtttatc actcgatgac tgtctcatca aactttccaa cgagggtttg
    11701 caggaacgca tggttcagct ttatcccgac gaggcggaac gggcggcaaa aatgccggaa
    11761 tatcaggagc ggctggactt tgagctgaac atcatcatcc aaatgaaatt ccccggctat
    11821 ttccttatcg tacaagactt tatcaactgg gcgaaaacgc acggctgtcc ggtcgggccg
    11881 ggacgcggtt cgggcgcggg ttcgctggtg gcgtattcgc tgaagattac cgacctcgac
    11941 ccgctgaaat acgcgctgct gttcgagcgt ttcctaaacc ccgaacgcgt ttccatgccc
    12001 gactttgacg tggacttctg tcaggcaaac cgcggccgcg tgattgaata cgtgcgcgaa
    12061 aaatacggcg ctcaggcggt cagccagatt gtgactttcg gcacgatgtc gtccaaagcg
    12121 gtcatccgcg acgtggggcg tgtgctggaa ctgccgttta tgctgtgcga caaactgtcc
    12181 aagctgattc cgttggaagc caacaaaccc ctgagtttgg acgacgcgat gaaggcgcag
    12241 ccgcagattc aggaattgct tgaagcggaa gaagcggacg aactgattac gctggcgaaa
    12301 aaactggaag acctgacgcg cggtttgggt atgcacgcag gcggcgtgtt gattgcgccg
    12361 ggcaagattt ccgattacag ccccgtgtat caggcggacg aatccgcctc gcccgtatcc
    12421 atgtacgaca agggcgacgt ggaagacgtg ggtttggtga agttcgactt tttgggcctg
    12481 cgcaacctga ccattatcga aatggcgcag aacaacatca aaaacaccgc cggcgatatc
    12541 gtcgatgtcg gcaagattcc gcttgacgac cagactgcct accaaatctt ccgcgatgcg
    12601 aacaccaccg ccgtcttcca gttcgagtcg accggcatga aaaaaatgct gaaaacggcg
    12661 catacgacca agtttgaaga actcatcgcc ttcgtatcgc tctaccgacc cggcccgatg
    12721 gacaacattc ccgacttcgt cgcacgcatg aagggacagg aattccaata catccacccg
    12781 ctgctggaag gcatcctcgc gccgacctac ggcattatgg tgtatcagga acaagtgatg
    12841 caggcggcgc agattatcgg cggctactcg ctcggcggcg cggacctgct gcgccgcgcc
    12901 atgggtaaga aaaagcccga agagatggtg aaacaccgcg aaatcttcgc cgaaggtgcg
    12961 gcaaaacaag gcatttcgcg cgaaaaatcc gacgaaatct tcaactacat ggaaaaattc
    13021 gccggctacg gtttcaacaa atcccacgcc gccgcctacg ccctgatttc ctaccagacc
    13081 gcatggctca aagcccacta tcccgccgaa tttatggcgg cgacgatgtc gtccgaattg
    13141 gacaacaccg accagctcaa acatttctac gacgactgcc gcgccaacgg catcgagttc
    13201 ctgccgcccg acattaacga atccgactac cgcttcacgc cgtatccgaa catgaaaatc
    13261 cgctacgcac tcggcgcgat taaaggcacg ggcgaagccg ccgtcgaatc catcatcgcc
    13321 gcgcggcaaa gcggcggcaa atttaccggc ctgttggact tctgcgagcg cgtcggcaaa
    13381 gaacacatga accgccgcac cctcgaagcc ctgatacgcg gcggcgcgtt cgacagcatc
    13441 gaacccaacc gcgccatgct cttggcgaac atcgaccttg ccatggacaa cgccgaccaa
    13501 aaagccgcca acgccaatca gggcgggctg tttgacatga tggaagacgc catcgaaccg
    13561 gtgcggctca tcgacgcacc catgtggagc gaatcggaaa aactcgccga agaaaaaacc
    13621 gtcatcggct tttacctgtc cggccacccg ttcggcccgt atgcccaaga agtccgccaa
    13681 atcgccccga ccaaattagg ccgtctgaaa ccgcaagaca gcgtgcgcct cgccggattc
    13741 gttaccgccg tgcgcaccat gatgggcaaa cgcggcaaaa tcgccttcgt cagcctcgaa
    13801 gattcgagcg ggcaggctga aatcatggtc ggcggacaaa cgctggaaaa ctgcgccgac
    13861 tgcctcaaag ccgaccaagt gctgattatc gaatccaaag tcagccgcga cgattacggc
    13921 ggcggcgacg ggctgcgcat catggcaaac caagtcatga ccctgcaaac ggcgcgcgaa
    13981 cgctacgccc gcagcctcag cctcgccctt gccccgcatc acgacatcgg cggactggta
    14041 cggctgctcg ccgcccacca actgcccgac acgccgcgca tcccgcttca attgtcgtat
    14101 gccaacgaaa aagcgtcggg caggcttcaa gtgcctccga aatggacggt tacaccgagt
    14161 tccgcattgt tcggcgaact ggaaacattg ctcggcagcc ggtcggtgcg cgtgaattgg
    14221 taaccaaaaa tacaaatgcc gtctgaagcc caaaaccggt ttcattcgta ctttattcga
    14281 atgattgaat aaaagtaact gccaagaaaa acgtattttt tggttatttc gccagcctaa
    14341 ataaatcaac cggggcgatt gatatccgtg tacatgacac agacagcgcc aaagggaaaa
    14401 acggcatttt caaaagtatc ggtgtcaaaa ccgccctttc attccaaaaa taccaaatcg
    14461 acgaaccggg caaagaaatc agaccgtgcc gcctgaaaaa acgcccgccc gtccgttaaa
    14521 ttaatctatc cctgtttcag gcggcctgaa acagggattt ttatatcaag ataaaatgag
    14581 aacgggagcg acaaccacgg gcaaagtcgg cgcgaactat caaaaattgt agcactgcga
    14641 aatgagaacg ggagcaataa cccttaggta gctcttgtta tagcggatta acgaaaatca
    14701 ggacaaggcg acgaagccgc agacagtaca aatagtacgg aaccgattca cttggtgctt
    14761 cagcacctta gagaatcgtt ctctttgagc taaggcgggg caacgccgta ccggtttttg
    14821 ttaatccgct atattttccg atgcaaaaca aagcagtcat atatttgatt ccccctaccg
    14881 ttgccacgct tccctcaaat attcgacgca gtcggtcagc gagtaaaacg ggacattgcc
    14941 gtggtcggca ttcggatatt cccggaaaaa tacggcggtc ccgcgcctgt ccaactctgc
    15001 cgccatttgt tcggcctgcc ctgccatgtc gcgttcttcc ctgcgtttgc aaccgctgct
    15061 ccgctcgagt gcgccgatgt tgaggcaggc atcgatgccg tccagccggt tttcagacgg
    15121 cataaagtcg agtatccgcc tgttgtgcca ccaaatcgag gggatacgag ccaatgccgt
    15181 ctgaaacagc ggtgggaaag cagggaatac agtccgaaca gtgcgccgaa cgagtgtccg
    15241 aatacggcgg tttcattacg gttaagggtg tagcggcttt ctaaaaaggc ggtcagttcg
    15301 ctgtcgataa agtcggcgaa gcggtctgcc tgtccgaact gccgccgttc gtctgctgtg
    15361 gcgttgtctc caagcggcgg cgtgtagtcg gcggcacgct gcgccaggtc gcgcacactg
    15421 cctgtcgtgt agccgatacc gacaatcagg cagggggcgt tgcttcgggt aacggggttg
    15481 ttcatcagcg actgcatgat gttgtacagc gcgggaaaaa ggcttcgccg tcgaggacaa
    15541 agaggacggg atagccttca gactgcattt cgccgagtgt tgccgtctga atccgataga
    15601 ttcgccccgt gcaggtggat ttgatttcgg tttcaaaggc ttggggcagt atggcaggtt
    15661 ggaatgtgtc ggtcggtatg ggtttcatga tgttcggctt gtgggtcaga ctggcggcaa
    15721 gcggtaaaac cgaaccggca aagcggtctg cctgccggtt cggtctgttt tccttcgcaa
    15781 tgccatactc cggttgtgag cgcatagggt atgccgcgca gcttgttgtg gtttgattat
    15841 gctgcggctg ccgtttcaga cggcatattg gtctttaaaa actgtaacgc aggtttgccg
    15901 tcaggctgcg ctccgaaccg ggaatgttaa aggtgttcgt gccgccgacg cgggcgtagt
    15961 aatggcggtt gaagatgttg tcggcattga tttgcagctt cagcttgggc gtgaagcggt
    16021 atgccgccat cgcatcgaac gtggcataac cgcctgcatg catccctgca gatgaagtaa
    16081 tgccgctcat cgcgttcacg ccgccgccga cggtcagccc gggcgtaact tggtaagtcg
    16141 tccacaggtt tgcgctgtgt ttgggcacca gcaggaagat gccgtcgtcg cgtggattgg
    16201 cggcggtttt gatttggctg tgcagatagc tgtaacctgc atggatttgc catttcggtg
    16261 taatcgcacc gctgatttcg gtctcaacgc cttccatcac gcgtttgccc aatgcggcgt
    16321 aacgggtgtt gcggttgttc gggttcggcg gcgtttttgt ctttcaggcg gcagggggag
    16381 acgcgggtat tgaggcggtc atccatatag ctgcctttaa cgagctgtaa tcgccgtttt
    16441 tctcggtggc aatcgcgttg tacgaacgct gccctttgat atggacggtt tccaaatccg
    16501 ccgtttgtgc ggcaaaaacc ggagacgaga gtgctgccaa aaccgtggcg gcggtcatat
    16561 tgatgcggaa aactgacata aactgtccca tctgtataaa tgataatggt tctattttaa
    16621 taaagcacaa cgcggcttgt tcggaaaaac atatcgctca gccgacaaat tttgtcgaaa
    16681 atgcgacacg tctgcgtttt ccgcataaaa tctgcttttt tactgcaacc aacctgccat
    16741 gaccccgccc aaactcatca tcttcgactg ggacggcacg cttgccgata cgacccaacc
    16801 catcatcgac accatgcgcc gcagcttcgc cgaatgcggt tttccgccgc ccgaagcgga
    16861 gcgcgtccgc agcctgatcg gatacagcct gcccgaaatc atccgcgccc tgctcgaaat
    16921 gccttctgaa gctgccgttg ccgacatcgc gcgcacttat tccgcgcatt acctcaatcc
    16981 caacaaccgc aatatgacac tctttcccga tgccccgccc tgtctggaca agctcaaagc
    17041 gcaaggcttc cggcttgccg tcgccacggg caaagggcgg gcgggtttgg acaacgccat
    17101 cagccaaacc gccaccggcg gctattggct cgccaccgcc tgcgcggggg aatacccctc
    17161 caaaccctcg cccgaaatgg tattcggact ctgcggcgaa ctgggactcg acccgaaaga
    17221 ggcattggtt gtcggcgata cggcgcacga cctgcatatg gcggcaaacg caggcgcggc
    17281 ggcagtcggc gttgccaccg gcgcacattc gcgcgaacag ctccttggcg caccgcatct
    17341 cgccgtattg gacggtttgt ccgaactgcc cggttttctt gcacgacatt acgcctgatt
    17401 ggtttccgca tccggcacgc ggcaaaaatg ccgtctgaag cctgttcaga cggcatgagg
    17461 cctttgcaaa aa
//
LOCUS       DBJGGI010000042        17460 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.042, whole genome shotgun sequence.
ACCESSION   DBJGGI010000042 DBJGGI010000000
VERSION     DBJGGI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17460
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.042"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..420
                     /locus_tag="ACSIGJ_001377"
     CDS             <1..420
                     /locus_tag="ACSIGJ_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HHQ1330219.1"
                     /translation="RTDGEGYIEKLHITPANAHECKHLSPLLEGIAEGTTVYADKGYD
                     SAENRQHLKEHQLQDGIMRKACRNRPLTEVQTKRNRYLSKTRYVVEQSFGTLHRKFRY
                     ARAAYFGLLKVSAQSHLKAMCLNLLKAANRLSVPAAA"
     gene            873..1517
                     /gene="rplC"
                     /locus_tag="ACSIGJ_001378"
     CDS             873..1517
                     /gene="rplC"
                     /locus_tag="ACSIGJ_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HHQ1330220.1"
                     /translation="MTLGLVGHKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            1517..2137
                     /gene="rplD"
                     /locus_tag="ACSIGJ_001379"
     CDS             1517..2137
                     /gene="rplD"
                     /locus_tag="ACSIGJ_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HHQ1330221.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            2134..2448
                     /gene="rplW"
                     /locus_tag="ACSIGJ_001380"
     CDS             2134..2448
                     /gene="rplW"
                     /locus_tag="ACSIGJ_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HHQ1330222.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLASATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            2454..3287
                     /gene="rplB"
                     /locus_tag="ACSIGJ_001381"
     CDS             2454..3287
                     /gene="rplB"
                     /locus_tag="ACSIGJ_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HHQ1330223.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGISAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGVVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVDCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            3293..3571
                     /gene="rpsS"
                     /locus_tag="ACSIGJ_001382"
     CDS             3293..3571
                     /gene="rpsS"
                     /locus_tag="ACSIGJ_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HHQ1330224.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAVRASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3580..3909
                     /gene="rplV"
                     /locus_tag="ACSIGJ_001383"
     CDS             3580..3909
                     /gene="rplV"
                     /locus_tag="ACSIGJ_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HHQ1330225.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            3919..4611
                     /gene="rpsC"
                     /locus_tag="ACSIGJ_001384"
     CDS             3919..4611
                     /gene="rpsC"
                     /locus_tag="ACSIGJ_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HHQ1330226.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVIIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPIHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4595..5011
                     /gene="rplP"
                     /locus_tag="ACSIGJ_001385"
     CDS             4595..5011
                     /gene="rplP"
                     /locus_tag="ACSIGJ_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HHQ1330227.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            5011..5202
                     /gene="rpmC"
                     /locus_tag="ACSIGJ_001386"
     CDS             5011..5202
                     /gene="rpmC"
                     /locus_tag="ACSIGJ_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HHQ1330228.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            5202..5465
                     /gene="rpsQ"
                     /locus_tag="ACSIGJ_001387"
     CDS             5202..5465
                     /gene="rpsQ"
                     /locus_tag="ACSIGJ_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HHQ1330229.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHSLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5687..6055
                     /gene="rplN"
                     /locus_tag="ACSIGJ_001388"
     CDS             5687..6055
                     /gene="rplN"
                     /locus_tag="ACSIGJ_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HHQ1330230.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAVPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            6067..6390
                     /gene="rplX"
                     /locus_tag="ACSIGJ_001389"
     CDS             6067..6390
                     /gene="rplX"
                     /locus_tag="ACSIGJ_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HHQ1330231.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGGKVVVEGVNVVKRHQ
                     KPNPMRGIKGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            6400..6939
                     /gene="rplE"
                     /locus_tag="ACSIGJ_001390"
     CDS             6400..6939
                     /gene="rplE"
                     /locus_tag="ACSIGJ_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HHQ1330232.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLERIAGQRPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            6942..7247
                     /gene="rpsN"
                     /locus_tag="ACSIGJ_001391"
     CDS             6942..7247
                     /gene="rpsN"
                     /locus_tag="ACSIGJ_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HHQ1330233.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            7263..7655
                     /gene="rpsH"
                     /locus_tag="ACSIGJ_001392"
     CDS             7263..7655
                     /gene="rpsH"
                     /locus_tag="ACSIGJ_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HHQ1330234.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7669..8202
                     /gene="rplF"
                     /locus_tag="ACSIGJ_001393"
     CDS             7669..8202
                     /gene="rplF"
                     /locus_tag="ACSIGJ_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HHQ1330235.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKKLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVASEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            8216..8569
                     /gene="rplR"
                     /locus_tag="ACSIGJ_001394"
     CDS             8216..8569
                     /gene="rplR"
                     /locus_tag="ACSIGJ_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HHQ1330236.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8588..9106
                     /gene="rpsE"
                     /locus_tag="ACSIGJ_001395"
     CDS             8588..9106
                     /gene="rpsE"
                     /locus_tag="ACSIGJ_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HHQ1330237.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            9099..9284
                     /gene="rpmD"
                     /locus_tag="ACSIGJ_001396"
     CDS             9099..9284
                     /gene="rpmD"
                     /locus_tag="ACSIGJ_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HHQ1330238.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTS
                     ENRGMINKISYLLKVES"
     gene            9286..9720
                     /gene="rplO"
                     /locus_tag="ACSIGJ_001397"
     CDS             9286..9720
                     /gene="rplO"
                     /locus_tag="ACSIGJ_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HHQ1330239.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTVSANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEDVGGKIEM"
     gene            9732..11042
                     /gene="secY"
                     /locus_tag="ACSIGJ_001398"
     CDS             9732..11042
                     /gene="secY"
                     /locus_tag="ACSIGJ_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HHQ1330240.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALLIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRVGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            11047..11265
                     /gene="infA"
                     /locus_tag="ACSIGJ_001399"
     CDS             11047..11265
                     /gene="infA"
                     /locus_tag="ACSIGJ_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HHQ1330241.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            11286..11399
                     /gene="rpmJ"
                     /locus_tag="ACSIGJ_001400"
     CDS             11286..11399
                     /gene="rpmJ"
                     /locus_tag="ACSIGJ_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HHQ1330242.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDPRHKQRQG"
     gene            11465..11827
                     /gene="rpsM"
                     /locus_tag="ACSIGJ_001401"
     CDS             11465..11827
                     /gene="rpsM"
                     /locus_tag="ACSIGJ_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HHQ1330243.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            11847..12242
                     /gene="rpsK"
                     /locus_tag="ACSIGJ_001402"
     CDS             11847..12242
                     /gene="rpsK"
                     /locus_tag="ACSIGJ_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HHQ1330244.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            12262..12882
                     /gene="rpsD"
                     /locus_tag="ACSIGJ_001403"
     CDS             12262..12882
                     /gene="rpsD"
                     /locus_tag="ACSIGJ_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HHQ1330245.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            12908..13894
                     /locus_tag="ACSIGJ_001404"
     CDS             12908..13894
                     /locus_tag="ACSIGJ_001404"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HHQ1330246.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVGGVQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKSPPIDPVLLHPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            13918..14286
                     /gene="rplQ"
                     /locus_tag="ACSIGJ_001405"
     CDS             13918..14286
                     /gene="rplQ"
                     /locus_tag="ACSIGJ_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HHQ1330247.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLASRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            14433..15146
                     /gene="minC"
                     /locus_tag="ACSIGJ_001406"
     CDS             14433..15146
                     /gene="minC"
                     /locus_tag="ACSIGJ_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HHQ1330248.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAVAMKYHLLFCL
                     SHSENVKELGQVEVQKTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVNQGAEL
                     IADGNMHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            15175..15990
                     /gene="minD"
                     /locus_tag="ACSIGJ_001407"
     CDS             15175..15990
                     /gene="minD"
                     /locus_tag="ACSIGJ_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HHQ1330249.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSRKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILRIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            15994..16257
                     /gene="minE"
                     /locus_tag="ACSIGJ_001408"
     CDS             15994..16257
                     /gene="minE"
                     /locus_tag="ACSIGJ_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HHQ1330250.1"
                     /translation="MSLIELLFGRKQKTATVARDRLQIIIAQERAQEGQTPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            16261..17181
                     /gene="oxyR"
                     /locus_tag="ACSIGJ_001409"
     CDS             16261..17181
                     /gene="oxyR"
                     /locus_tag="ACSIGJ_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HHQ1330251.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLKEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIHD"
BASE COUNT         4821 a         3307 c         4346 g         4986 t
ORIGIN      
        1 cgtaccgatg gggaaggcta tatcgagaaa ctgcacatca cccccgccaa tgcccatgag
       61 tgcaaacacc tgtcaccgtt gttagaaggg atagctgaag gtacgaccgt ctatgccgac
      121 aaaggctacg acagtgcgga aaaccggcaa catctgaaag aacatcagtt gcaggacggc
      181 attatgcgca aagcctgccg caaccgtccg ctgacggaag tgcaaaccaa acgcaaccga
      241 tatttgtcga agacccgtta tgtggtcgaa caaagcttcg gtacgctgca ccgtaaattc
      301 cgctatgcgc gggcagccta tttcgggctg ctcaaagtga gtgcgcaaag ccacctgaag
      361 gcgatgtgtt tgaacctgtt gaaagccgcc aacaggctaa gtgtgcccgc tgccgcctaa
      421 aaggcagccc ggatgcctga ttatcgggta tccgggcagg attaaggggg tgtttgggta
      481 aaattaggcg gtatttgggg cgaaaacagc cgaaaacctg tgtttgggtt tcggttgttg
      541 gagggaaagg aattttgcaa aggtctcagc atacgaaaac taccatctca aagaaataat
      601 ggatgcagtc atcaaccata ttccggtttg tcggagaaag atgcatacgc tgtgatgacc
      661 ggataccgac cctttaaaag agtccgaccc tatgccgtct gaaaattcaa aacgcttcag
      721 acggcatatt gaagatattt ctgatatttc tgttgatatt tctttgactt gtcagatata
      781 atgccgagct tggtacattt gtgccaagtt taactttgtc tgaaagacag gccaatcgta
      841 gcctgtccct ttactttaaa aggaaaataa tcatgacttt aggtctggtt ggacacaaag
      901 ttggtatgac ccgcgtgttc gacgaacagg gtgtttctgt tccggtaacc gttttggata
      961 tgtctgccaa ccgcgttaca caagtaaaat ccaaagatac tgacggctat actgccgttc
     1021 aagttacctt tggtcagaaa aaagccaatc gtgtcaacaa agccgaagcc gggcactttg
     1081 caaaagcagg tgttgaagcc ggtcgcggtt tgattgagtt tgctttgact gaagaaaaac
     1141 tggctgaatt gaaagctggt gacgaaatca ccgtttctat gtttgaagtc ggtcaactgg
     1201 tcgatgtaac cggtacctct aaaggtaaag gtttctccgg cacgattaaa cgtcataact
     1261 tcggtgccca acgtacttcc cacggtaact cccgttctca ccgtgttcca ggctctatcg
     1321 gtatggcgca agacccgggt cgcgtgttcc ccggtaaacg catggccggc caatacggca
     1381 acaccaaagc aactgttcaa aaattggaag ttgtccgtgt tgatgcagaa cgccaactgc
     1441 tgttggttaa gggtgctgtt ccgggtgcgg tcaacagcga tgttgtagtt cgtcccagcg
     1501 tgaaagtagg tgcgtaatgg aattgaaagt aattgacgct aaaggacaag tttcaggcag
     1561 cctgtctgtt tctgatgctt tgttcgcccg cgaatacaat gaagcgttgg ttcatcagct
     1621 ggtaaatgcc tacttggcaa acgcccgctc tggtaaccgt gctcaaaaaa cccgtgccga
     1681 agtaaaacac tcaaccaaaa aaccatggcg tcaaaaaggt accggccgcg cccgttccgg
     1741 tatgacttca tctccgctgt ggcgtaaagg tggtcgcgcg ttcccgaaca aacccgacga
     1801 aaacttcact caaaaagtaa accgcaaaat gtaccgtgcc ggtatggcga ctattctgtc
     1861 ccaattgact cgtgacgagc gtttgtttgc gattgaggcg ttgactgccg aaactcctaa
     1921 aaccaaagtt tttgctgaac aagtgaaaaa tctgggtctg gagcaagtgt tgtttgtaac
     1981 caaacagctc gacgagaatg tttacttggc ttcacgcaac ttgccaaacg tgttggtttt
     2041 ggaagctcaa caagttgatc cttacagctt gctgcgttac aaaaaagtaa tcatcactaa
     2101 agatgcagtt gcacaattag aggagcaatg ggtatgaatc aacaacgttt gactcaagtg
     2161 attttggcac ctatcgtttc tgaaaaaagc aacgtattgg ctgaaaaacg caaccaaatg
     2221 acgtttaaag tcttggcaag tgcaaccaaa cctgaaatca aagcggctgt cgagctgctg
     2281 tttggtgttc aagttgcttc tgtaactacc gttacaacta aaggcaaaac taagcgtttt
     2341 ggtcgtactt tgggccgccg cagcgatgtt aaaaaagctt atgtaagctt ggctgccggt
     2401 caagagttgg atttggaagc cgctgctgca gctgcagata aggaataaac aaaatggcaa
     2461 tcgttaaaat gaagccgacc tctgcaggcc gtcgcggcat ggttcgcgtg gtaacagaag
     2521 gtttgcacaa aggtgcacct tatgcgccct tgcttgaaaa gaaaaattct actgccggcc
     2581 gtaacaataa tggtcatatc accacccgtc acaaaggcgg cggtcataaa caccattacc
     2641 gtgttgtaga ctttaaacgt aacaaagacg gcatttctgc taaagtagag cgtatcgaat
     2701 acgatcctaa ccgtactgcc ttcatcgcac tgttgtgcta tgcagacggc gagcgtcgtt
     2761 acatcatcgc tcctcgcggt attcaagccg gtgtcgtatt ggtttccggt gctgaagctg
     2821 ccatcaaagt aggcaacacc ctgccgatcc gcaacattcc cgttggtacg actatccact
     2881 gtatcgaaat gaaacccggt aaaggtgctc aaatcgcacg ttctgccggt gcttctgcgg
     2941 tattgttggc taaagaaggt gcatacgctc aagtccgtct gcgctctggc gaagttcgta
     3001 aaatcaacgt agattgccgt gcgaccatcg gtgaagtcgg taacgaagag caaagtctga
     3061 aaaaaatcgg taaagccggt gctaaccgtt ggcgcggtat tcgtccgacc gtacgcggtg
     3121 ttgtcatgaa tcccgtcgat cacccgcatg gtggtggtga aggccgtacc ggcgaagccc
     3181 gcgaaccggt tagtccatgg ggtactcctg ctaaaggcta ccgcactcgt aataacaaac
     3241 gcacggataa tatgattgtt cgtcgccgtt actcaaataa aggttaatta gtatggctcg
     3301 ttcattgaaa aaaggcccat atgtagacct gcatttgctg aaaaaagtag atgctgttcg
     3361 cgcaagcaac gacaaacgcc cgattaaaac ctggtctcgt cgttctacca ttctgcctga
     3421 ttttatcggt ctgaccattg ccgtgcacaa cggtcgcacc catgtgcctg tgtttatcag
     3481 cgacaatatg gttggtcata aattaggcga attctcattg acccgtacct ttaaaggcca
     3541 cctggccgat aaaaaggcta aaaagaaata aggtgaatca tgagagtaaa tgcacaacat
     3601 aaaaatgccc gtatctctgc tcaaaaggct cgtttggtag ctgatttgat tcgtggtaaa
     3661 gacgttgccc aagctttgaa tattttggct ttcagcccta aaaaaggtgc cgagctgatc
     3721 aaaaaagtat tggagtcagc tattgctaat gctgagcata ataacggtgc ggacattgat
     3781 gaactgaaag tggtaactat ctttgttgac aaaggtccaa gcttgaaacg ttttcaagct
     3841 cgcgccaaag gtcgcggtaa ccgcatcgaa aaacaaactt gtcatatcaa tgtgacagtg
     3901 ggtaactaag gaaaagctat gggacaaaag attaacccta caggctttcg cctggcggta
     3961 actaaagact gggcttcaaa atggtttgct aaaagtaccg acttttctac tgttttgaag
     4021 caggatatcg atgttcgcaa ttatttgcgt caaaaattgg ccaatgcttc ggttggtcga
     4081 gtaattattg aacgccctgc aaaatctgca cgcattacca ttcactccgc tcgtccgggt
     4141 gtggttatcg gtaaaaaagg tgaggatatc gaagttttga aacgtgactt gcaagtcttg
     4201 atgggtgtac ctattcatgt aaatattgaa gagattcgcc gtcctgagtt ggatgctcaa
     4261 attattgctg atggtatcgc ccagcagttg gaaaaacgcg ttcaattccg tcgtgctatg
     4321 aaacgagcaa tgcaaaatgc aatgcgttct ggtgctaaag gcattaagat tatgacttca
     4381 ggccgtctga atggtgcgga tattgcccgt agcgaatggt atcgtgaagg tcgcgtgcca
     4441 ctgcatactt tacgtgcaaa tgtagattat gcaaccagcg aagcgcacac cacatatggt
     4501 gtattgggtc tgaaagtttg ggtttatacg gaaggcaata ttaaatcttc caaacctgaa
     4561 catgagagta aacaaagaaa ggcaggtaga cgtaatgctg cagccaacta gactgaaata
     4621 ccgtaagcaa caaaagggtc gcaataccgg catcgctact cgcggtaata aggtaagttt
     4681 cggtgagttc ggcttgaaag ccgtaggtcg tggtcgtttg actgcccgtc aaatcgaagc
     4741 tgctcgtcgt gcaatgaccc gtcacatcaa acgtggtggt cgtatttgga ttcgtgtatt
     4801 ccctgacaaa ccgattactg aaaagcctat tcaagttcgt atgggtggcg gtaaaggtaa
     4861 cgtggaatat tacattgccg aaattaaacc aggtaaagtg ttgtatgaaa tggatggcgt
     4921 tccagaggaa ctggctcgtg aagcattcga gttggctgct gccaaattgc ctattcctac
     4981 aacctttgta gtaagacagg tgggtcaata atgaaagcaa atgaattgaa agacaaatcc
     5041 gttgagcagt tgaatgcaga tttgttggac ttgttgaaag ctcagtttgg cttacgtatg
     5101 caaaacgcta ccggtcaatt aggcaaacca agtgaattga aacgtgtacg tcgcgatatt
     5161 gctcgtatta aaaccgtttt aactgaaaaa ggtgctaagt aatgagcgaa actaaaaatg
     5221 ttcgtacttt gcaaggcaaa gtagtaagcg acaaaatgga taaaaccgta acagtattgg
     5281 ttgagcgtaa agtaaaacat tcgctgtatg gtaagattat tcgattatct actaaaatcc
     5341 atgcccatga tgaaaataat caatatggaa ttggtgatgt ggttgttata tcggaatccc
     5401 gtccattgtc aaaaactaaa tcttgggttg tcagtgagct ggttgagaaa gcacgttcta
     5461 tttaagaatt aaagcaacgt gcttggaatg ggaaacgaag tattgcagca aatttaattt
     5521 gcgtgtaaac ttcgtttcct gtctttcagt ttcttctgga agtttcttcc ctttcgggat
     5581 ccaagactgg tttacttgaa ccgcaaggtt tcatttaata agcagcggct ttgctgtaag
     5641 ttatctgaaa gtggtaaatt aagttggtta atttaaaggt aataatatga ttcaaatgca
     5701 gaccatctta gatgtggctg ataactctgg tgcgcgtcgc gtaatgtgta tcaaggtatt
     5761 gggcggatct aagcgtcgct acgcttctgt tggcgatatt attaaagtgg cagttaaaga
     5821 tgcggttccg cgtggccgtg tcaaaaaagg cgatgtatat aatgcggtag ttgttcgtac
     5881 tgctaagggt gtgcgtcgtc ctgatggtgc gttaattaaa ttcgataaca atgccgccgt
     5941 gttgctgaat aataaacttg aacctttggg tactcgtatc tttggtccgg taacccgtga
     6001 attgcgtact gagcgattta tgaaaatcgt ttcattggca cctgaagtat tataaggaat
     6061 agcacgatga ataaaatcat taaaggcgat agggttgtag taattgctgg taaggataaa
     6121 ggtaagcagg gtcaagtagt tcgagtgttg ggtggtaaag ttgttgttga gggcgttaat
     6181 gttgtaaaac gccatcaaaa acctaatcca atgcgtggca ttaagggcgg tattattact
     6241 aaagaaatgc ctttggatat ttctaatatc gcaatcctga atccggaaac taataaagcg
     6301 gaccgtgttg gtattaagct gattgaaaat gaaggcaaag ttaaacgcgt tcgtttcttc
     6361 aaatcaaatg gctctatcat tggagcataa ggagataaca tggctcggtt gagagagttt
     6421 tataaagaga cagttgttcc tgaattggtt aaacaatttg gttacaaatc agtaatggaa
     6481 gtcccgcgta ttgaaaaaat caccttgaat atgggtgtgg gtgaggctgt tgctgacaaa
     6541 aaagttatgg aacatgctgt ttccgattta gagagaattg ccggtcaaag accggttgtt
     6601 actgttgccc gtaaatctat cgcaggtttt aaaatccgtg ataactatcc ggttggttgc
     6661 aaagtaacat tgcgtcgtga tcaaatgttt gaattcttgg atcgtttgat tactattgca
     6721 ttacctcgcg tacgtgactt ccgtggtgtg agcggtaaat catttgatgg ccgtggcaat
     6781 tacaatatgg gtgttcgtga gcaaattatt ttcccggaaa ttgaatacga taaaattgat
     6841 gctttgcgtg gtttgaatat tactattact actacagcaa aaaccgatga ggaagcgaaa
     6901 gctttattgt cattgtttaa atttccgttc aaaggataat catggctaag aaagcactta
     6961 ttaatcgtga tctgaaacgt caagctttgg ctaaaaaata tgcggctaaa cgcgcggcaa
     7021 ttaaagcggt aatcaatgat tcgaatgcaa ctgaggaaga gcgttttgag gctcgtttga
     7081 ggtttcaatc cattcctcgt aatgcggcac ctgtgcgtca acgtcgtcgt tgtgctttga
     7141 caggtcgccc tcgtggtact ttccgtaaat ttggtttggg tcgtattaaa atccgtgaaa
     7201 tcgccatgcg tggcgaaatt ccgggtgttg ttaaagccag ctggtaatag gagtaattaa
     7261 gaatgagtat gcatgatcct atttccgata tgttgactcg tatccgcaat gcgcaacgtg
     7321 ctaataaagc agcagttgca atgccttctt caaaattaaa gtgtgctatt gcaaaggtat
     7381 tgaaagaaga aggatatatt gaggacttcg cagtttcatc tgacgtaaag tctatattgg
     7441 aaattcaatt aaaatactat gcaggtcgtc ctgtaattga acaaatcaag cgtgtatctc
     7501 gccccggttt gcgtatttat aaagcgtcta gtgagattcc aagtgttatg aatggcttgg
     7561 gtattgctat cgttagtact tctaaaggtg taatgactga tcgtaaagca cgttctcaag
     7621 gtgttggtgg tgagttgtta tgcattgtag cctagtggag gaaaagaaat gtcacgtgtc
     7681 gcaaaaaacc cagtgacggt tcccgctggt gtagaagtaa aatttggaac agaggcattg
     7741 gttattaagg gtaagaacgg tgaattgtct tttcctttgc attctgatgt agccattgaa
     7801 tttaatgatg gcaaattgac ttttgttgcg aataatagca gtaaacaagc aaatgcaatg
     7861 tctggtactg ctcgcgcatt agtcagcaat atggttaaag gtgtttcaga aggttttgag
     7921 aagaaattac aattgatagg cgtgggttat cgtgctcaag cacaaggtaa aatcttgaat
     7981 ctgtctttgg gtttttctca tccgatcgta tatgaaatgc ctgaaggtgt ctccgttcaa
     8041 actcctagcc aaacagagat tgttttaact ggctcggata aacaagttgt tggtcaagtt
     8101 gcttctgaga ttcgtgcgtt ccgtgctcct gagccttata aaggtaaagg tgttcgctat
     8161 gtaggcgaag tagtggtaat gaaagaagcc aagaaaaaat aattgaggtt cactaatgga
     8221 taaacataca acccgactcc gtcgtgcacg caaaacccgt gctcgtattg cggacttgaa
     8281 aatggtaaga ttatgtgtgt tccgaagcaa taatcatatt tatgctcaag taattagtgc
     8341 tgaaggtgat aaagtattgg ctcaagcctc tacattggaa gctgaggtgc gcggtagtct
     8401 gaaatctgga agcaatgttg aagcagctgc aatagttggt aaacgtattg ctgagaaggc
     8461 taaagcagca ggtgtagaaa aggttgcttt tgatcgttca ggtttccaat atcacggtcg
     8521 tgtgaaggct ttggctgaag ctgctcgtga aaatggttta agcttctaaa tatttggaga
     8581 ctttcagatg gcaaaacatg aaattgaaga acgcggtgac ggtctgattg aaaagatggt
     8641 cgcagttaac cgtgtaacca aagtagttaa aggtggtcgc attatggctt tctctgcgct
     8701 aactgttgtt ggtgatggag atggtcgtat tggtatgggt aaaggtaagt caaaagaagt
     8761 gcctgttgca gtccaaaagg cgatggatca agcacgacgc tctatgatta aggtaccatt
     8821 aaaaaatggt acgatccatc atgaggttat tggtcgacat ggtgctacta aagtatttat
     8881 gcagcctgct aaagagggta gtggcgtaaa agccggtgga cctatgcgtt tggtttttga
     8941 tgctatgggc attcataata tttccgccaa agtgcacgga tctactaacc catataatat
     9001 cgtacgtgca acattagatg gtttgtctaa gttgcatact cctgctgata tcgcagccaa
     9061 acgtggcttg acagtggaag acattttggg agttaaccat ggctgaacaa aaaaagatta
     9121 gggttacatt ggttaaaagc ctgattggta caattgaatc tcatcgtgca tgtgcacgcg
     9181 gtttaggttt gcgtcgtcgc gagcatacgg tagaggtttt agatacctct gaaaaccgtg
     9241 gtatgattaa taaaatcagc tacttgttga aagtggagtc ttgatatgtt tttgaataca
     9301 attcaacctg ctgttggtgc tacgcatgct ggtcgtcgtg ttggacgcgg tattggtagt
     9361 ggtcttggca aaacgggtgg tcgtggtcat aaaggtcaaa agagccggtc tggtgggttt
     9421 cataaggtgg gtttcgaggg tggtcaaatg cccttgcaac gacgcctccc taaaagaggt
     9481 tttaaatctt taacagtatc agctaatgca cagcttcgtt taagtgaact ggaatcaatt
     9541 gctgttaatg agattgatat tttagtctta aagcaagcag gtctgattgc atctacagtc
     9601 tctaatgtta aagttattgc ttctggtgaa atttctaagg cagttgcttt gaagggtatt
     9661 aaagttacca aaggtgcgag agctgctatc gaggatgttg gtggtaagat tgaaatgtaa
     9721 ggtttaatat tgtggctaat caacaaacgt catcaggttc atccaagttt ggagatctta
     9781 agaaacgtct tttgtttcta tttggagcat tgattgtttt tcgaattggt gcccatatac
     9841 ccgtacctgg ggttgatgct gttgctttag ctaaattata cgaaagcgct ggaaacggca
     9901 tcctgggaat attgaatatg ttttccggtg ggtcgttaga gcgctttagt atatttgcaa
     9961 taggaattat gccatatatt tcagcttcta ttattgtaca gctcgcttct gaaattttgc
    10021 catcattgaa ggctttaaaa aaagaagggg aggctggtag aaaggtaatt acgaaatata
    10081 ctaggtatgg tactgttttg ttagcaattc ttcaaagtct aggtgttgca tctttcgtat
    10141 ttcagcaagg aattgttgta acaagttcat ttgagtttca tgtttccacg gtagtttctt
    10201 tggtaacggg aaccatgttt cttatgtggc ttggggagca aattactgaa aggggtatcg
    10261 ggaacggtat ttctttaatc attacggcag gtattgcttc aggtattcct tcgggtattg
    10321 caaagctggt tacactgacg aaccaaggtt ctatgagcat gcttacggcg ttgcttattg
    10381 tatttggtgc cttattatta atttatttgg ttgtatactt tgaaagtgca cagcggaaga
    10441 ttcctattca ttatgcaaaa cgccagttta atggtagggt gggtagtcaa aatacgcata
    10501 tgcctttcaa gttgaatatg gctggtgtta ttcccccaat ttttgcttcc agtattattc
    10561 tatttccatc tactctttta ggttggtttg gttcggctga tacaaatagt gttttgcaca
    10621 aaatagctgg attgttacaa cacggtcaat tgctgtatat ggctttattt gcagcgacag
    10681 ttattttctt ttgttatttt tatacggctt tggtttttag tcctaaagaa atggcagaga
    10741 atttaaaaaa gagtggtgct tttgttcctg ggattagacc tggtgagcag acctctaggt
    10801 atttagaaaa agttgtatta cgtttgacat tgtttggagc tctttatatt acaactattt
    10861 gtttaattcc agagttctta actacggttt taaatgtacc tttttatttg ggtggcacgt
    10921 ctttgttgat tctagttgtt gtaacgatgg attttagtac acaaataaat tcgtataggc
    10981 ttactcaaca gtatgataag ttaatgactc gttcagaaat gaaatcattt tctcggaaat
    11041 agaattatgg cgaaagaaga tactatccaa atgcaaggtg aaattcttga aactttacct
    11101 aacgcaacat ttaaagtaaa acttgagaat gaccatattg tattgggtca tatttctggg
    11161 aagatgcgga tgcattacat tcgtatttct ccgggagata aggtcacagt agagctgaca
    11221 ccttatgatt taactagggc tcgaatcgtt ttcagagcaa gataaaccaa taaaaggaaa
    11281 ataaaatgcg tgtacaacca tctgttaaga aaatttgccg aaattgcaag attattcgtc
    11341 gaaatcgtgt agttcgtgtg atttgtactg atccccgtca caaacagcgt caaggttaat
    11401 ggaatatttc ttttaatgtg attctgtgat atagtgacaa aatttgctct aaaaaggaaa
    11461 aaacatggct cgtattgcag gggtaaatat ccctaataac gcacacatcg taattggtct
    11521 tcaggctatt tacggtattg gtgctactcg tgctaaattg atttgtgagg ctgcaaatat
    11581 tgcgcctgat actaaagcaa aagatttgga cgagactcaa ttagatgctt tgcgtgatca
    11641 agttgccaag tatgaagtag aaggtgattt gcgtcgtgaa gtaactatga gtatcaaacg
    11701 attgatggac atgggctgct atcgtggctt ccgtcatcgt cgcggcttac catgccgcgg
    11761 tcaacgcact cgtacaaatg cgcgtactcg caaaggtccg cgtaaagcga ttgctggtaa
    11821 gaaataaatt ttaaggaatt ttattaatgg ctaaagcaaa cacagcttca cgtgtacgta
    11881 aaaaagtacg taaaaccgtg agtgagggta ttgtgcacgt tcatgcatct ttcaacaata
    11941 ccatcattac aatcactgac cgtcaaggca atgcgttgtc ttgggctacc tctggcggcg
    12001 ctggttttaa aggttctcgt aaaagtacac catttgcagc acaagttgca gcagaagcag
    12061 ctggtaaagt tgcccaagag tatggcgtta aaaatttaga ggttcgtatt aaaggtccag
    12121 gtccaggtcg cgaatcctct gtacgtgctt tgaatgctct tggtttcaag attaccagca
    12181 ttactgacgt taccccgttg cctcataacg gttgccgtcc gcctaaaaaa cgtcgtattt
    12241 aatattggag tgatttgaaa catggcacgt tatattggcc ctaaatgtaa gttggcacgt
    12301 cgcgaaggta cggatttgtt tttgaagagt gcgcgccgct ctttggattc taaatgtaaa
    12361 attgattccg ctcccggtca gcatggtgca aaaaaaccgc gtttgtcaga ctatggtttg
    12421 cagttgcgtg aaaaacaaaa aatccgccgt atttatggcg tattagaacg tcagttccgt
    12481 cgttatttcg cagaagctga tcgccgtaaa ggttctactg gcgagttgct gttgcagttg
    12541 ctggaatctc gtttggataa tgtcgtttat cgtatgggtt tcggttctac ccgagctgaa
    12601 gcaagacagc ttgtttctca taaggctata gttgtaaatg gacaagttgt caatattcct
    12661 tctttccaag tgaaagctgg tgatgttgtc tcagttcgtg aaaaagccaa aaaacaggta
    12721 cgtattcaag aagcattggg tttggcaact caaatcggct tgccgggttg ggtttctgta
    12781 gatgcagata aacttgaggg tgtgttcaaa aacatgccgg atcgctctga attgaccggt
    12841 gatattaatg aacagctggt ggtagagttc tactctaaat aatgctagct cagtgaggga
    12901 cagttaaatg cagaatagca caaccgaatt tttgaaacct cgtcaaattg atgtaaatac
    12961 tttttctgca actcgtgcaa aagtatctat gcagccattt gaacgtggtt tcggtcatac
    13021 cttaggtaat gctttgcgcc gtatcttact gtcatccatg aatggttttg ctcctactga
    13081 agtagctatt gccggtgtat tgcacgaata ttctactgtt ggtggtgttc aggaagatgt
    13141 tgttgacatt ttgctgaata ttaaaggtat tgtgtttaaa ctccatggtc gtagccaagt
    13201 tcaacttgtg ttgaagaaat caggttcagg tgtcgtatct gccggtgata ttgagttgcc
    13261 gcatgatgta gaaattctga atcctggtca tgtcatttgt catttggctg ataacggtca
    13321 aattgaaatg gaaattaaag tagagcaagg tcgtggttat caatctgttt caggtcgtca
    13381 ggtagttcgt gatgagaacc gtcagattgg tgcaatccag ttggatgcga gcttttcgcc
    13441 catcagccgt gttagctttg aggttgaacc tgcacgtgta gagcagcgga cggatcttga
    13501 taaattggtt ttggatatcg aaaccgacgg ttctattgat cctgaggaag ctgtacgcag
    13561 tgcggcacgt attttgattg atcagatgtc tatttttgct gatttgcagg gtactcctgt
    13621 ggaggaggtt gaagaaaaat cacctcctat cgaccctgtt cttttgcatc cggtggatga
    13681 tctggaattg acagtacgtt cagctaattg tttgaaagct gaggatattt attatattgg
    13741 cgatttgatt caacgcactg aaaccgagct tcttaaaacg ccgaatttgg gacgtaaatc
    13801 tttgaatgag attaaggaag tattggcatc taaaggtttg acactgggtt ctaagttgga
    13861 agcatggcca cctgtaggct tggaaaagcc ttaatgaaga attaaaggat aattgatatg
    13921 cgtcatcgta atggcaatcg caaattaaac cgtaccagca gtcatcgtgc tgcaatgctg
    13981 cgtaatatgg cgaattcatt attgactcac gaaactattg taacaactct gcctaaggcc
    14041 aaggaattgc gccgtgtagt agagccgttg attacattgg gtaaaaagcc gtcattggca
    14101 agccgccgtt tggcatttga ccgtactcgc gaccgtgatg ttgtagtaaa actgtttggc
    14161 gatttgggtc ctcgttttac tgctcgtaac ggtggttatg ttcgagtgtt gaaatacgga
    14221 ttccgtaaag gtgataatgc acctctggca ctggttgaat tggttgacaa accggctgct
    14281 gagtaatttt agtcatataa cgccatctgc cgaaaagcag gtggcgttat ttttgcaata
    14341 tctgatagat aatatggtat tggctattat gtttaaaata ttaattgaat agctaaggtt
    14401 tgcgcggtaa acttacatca ttaaaaaatt ctatgatggt ttatataatg aatgccttcg
    14461 atataaagtc gacaaagatg gacgtattgt ctatatcttt gcatacatca gacttgtttg
    14521 atttggaaga tgtgctggtc aaattgggca agaagtttca agagtctggt gttgttccat
    14581 ttgttctgga tgttcaagaa tttgattatc ccgagtcttt ggatcttgct gcattggttt
    14641 cgttgttttc aaggcatggt atgcaaattt tgggtctgaa gcattctaat gaacgttggg
    14701 ctgctgtggc tatgaagtat catttgctgt tttgtctgtc tcattcggaa aatgttaaag
    14761 aactgggtca ggttgaggtg cagaaaacgg aggatggtca gaaagcaagg aaaacagtat
    14821 tgattacatc ccctgtccgt accggtcagc aggtttatgc cgaagatggc gatttgattg
    14881 ttacgggggc ggtcaaccag ggggcggaat tgattgcaga tggcaatatg catatttatg
    14941 cgccgatgag agggcgtgct ttggccggcg ccaaaggtga tacttctgcc cgcatattta
    15001 tccactccat gcaggcggag ttggtttctg tcgcggggat ttaccgtaat tttgaacagg
    15061 atttgccgga ccatctgcac aagcagccgg tacagatatt gttgcaggat aaccgattgg
    15121 ttatcagtgc aattggctca gagtaattgt ttgatatttt aaaaaggaaa tattgtggca
    15181 aaaattattg tagtaacttc aggcaagggc ggtgtcggta aaacgactac cagtgcaagt
    15241 attgcaacag gtttggcatt acgcggatat aaaactgcgg taattgattt tgatgtgggt
    15301 ttgcgcaacc tcgacctcat tatgggttgc gagcgtcgtg tcgtttatga cctgatcaat
    15361 gtcattcagg gtgaggcgac gctcaaccag gctttgatta aagataaaaa ttgtgaaaac
    15421 ctgtttattt tgccggcttc ccagactcgg gataaagacg ctttgacacg cgagggcgta
    15481 gaaaaagtga tgcaggagct gtccggcaag aaaatgggct ttgagtatat tatttgcgac
    15541 tctcctgctg gtattgagca gggtgcattg atggcgttgt attttgctga tgaagccatt
    15601 gtaacgacca atcctgaggt ttccagtgtg cgtgactccg acaggatttt gggaattttg
    15661 caaagcaaat cccgtaaggc ggagcaaggc ggttcggtta aagaacatct gttaattacg
    15721 cgttattctc ccgaacgtgt ggcaaaaggc gaaatgctgt ctgtacagga tatttgcgat
    15781 attctgcgta ttcctttgct gggtgtgatt cccgaatccc aaaacgtctt gcaggcatcc
    15841 aattccggag aaccggtcat ccatcaggac agcgtagcgg cttccgaggc atataaggac
    15901 gttattgccc gtcttttggg cgagaaccgt gaaatgcgtt tcttggaagc tgagaaaaaa
    15961 agcttcttca aacgtctgtt cggaggataa ggtatgtcat tgatcgaact tttattcggt
    16021 agaaagcaga aaacggcaac cgttgcccgc gaccgccttc aaatcatcat tgcccaagag
    16081 cgcgcccaag aaggtcagac tccggattac ctgccgactt tacgtaaaga gttgatggaa
    16141 gtcctgtcca aatatgtgaa tgtttcatta gacaatatcc gtatttccca agaaaagcag
    16201 gatggtatgg atgtgcttga gttgaacatt actttgccgg aacagaaaaa ggtataggac
    16261 atgaccttaa ccgaattgcg gtacatcgtc gcagtcgccc aagaacgtca tttcggccgg
    16321 gcggcgcggc gttgttttgt cagccagccc actttgtcta ttgccattaa gaaattggaa
    16381 gaagagcttg ccgtctcttt gtttgaccgg agcagcaacg atattattac gaccgaggcg
    16441 ggggaacgta tcgttgcaca ggcgcgtaag gtattgaaag aggcggagct tatcaggcat
    16501 ttggcaaatg aagaacaaaa cgagctggag ggtgcgttca aactcgggct gatttttacg
    16561 gttgcgccat acctgctgcc gaaactgatt gtctctttgc gccgtactgc accgaaaatg
    16621 cctttgatgt tggaagagaa ttacacgcat actttgaccg agtcgctcaa acgcggggac
    16681 gttgacgcga ttatcgttgc cgaaccgttt caagagccgg gcattgttac cgaacccttg
    16741 tatgacgaac cgtttttcgt gattgtcccg aaagggcatt catttgagga actggatgcc
    16801 gtttcgcccc ggatgctggg tgaggagcag gttttgctgc tgacggaagg caactgtatg
    16861 cgggatcagg tactctcaag ctgttccgaa ttggcggcga aacagcgcat acaggggctg
    16921 accaatacat tgcagggcag ctcgatcaat acaatccgcc atatggtcgc cagcggtttg
    16981 gcaatcagcg tgttgccggc aaccgcgctg accgagaacg atcatatgct gttcagcatt
    17041 attccgtttg aaggtacgcc gccaagccgg cgggtcgtat tggcgtatcg ccgtaatttt
    17101 gtccgtccga aggcgttgtc ggcgatgaag gcggcgatta tgcagtcgca gcttcacggg
    17161 gtaagtttta tccacgacta ggcgcagaaa atgccgtctg aaacgggttt cagacggcat
    17221 ttgttatctt tgccgtcaat caggcaaacc tatccgacct acagttgctt cgaatcgtca
    17281 ttcccgcgca ggcgggaatc taggtctgtc ggtgcggaaa cttatcaggt aaaacggttt
    17341 ctccggtccc gagtcctaga ttcccacttt cgtgggaatg acggcatatt ttgacattga
    17401 ataaaaaaga ctaaaaacag aaaacagaaa acggaaaacg gaaaacggaa aacggaaaac
//
LOCUS       DBJGGI010000043        17003 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.043, whole genome shotgun sequence.
ACCESSION   DBJGGI010000043 DBJGGI010000000
VERSION     DBJGGI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17003
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.043"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..762
                     /locus_tag="ACSIGJ_001410"
     CDS             <1..762
                     /locus_tag="ACSIGJ_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HHQ1330252.1"
                     /translation="SLLFSSLLFSSAAQAASKDNGRSPYYVQADLAYAAERITHDYPQ
                     ATGANNTSTVSDYFRNIRAHSIHPRVSVGYDFGGWRIAADYASYRKWNNNKYSVNTKE
                     VQRNNNGNWQELKTENQENGSFHAASSLGLSAIYDFKLNDKFDKFKPYIGVRVAYGHV
                     KHQVHSVRKETTTVFSKPSGTTKPGEIPSLVTKPAYHESNSISSLGLGVIAGVGFDIT
                     PKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYRF"
     gene            complement(736..945)
                     /locus_tag="ACSIGJ_001411"
     CDS             complement(736..945)
                     /locus_tag="ACSIGJ_001411"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330253.1"
                     /translation="MPSERLSDGIFPCVLNRIHETPDAFGGNVGCRAMFGGDQRTKYA
                     GKGLMPSEGSDGIGVGESEAVAHAQ"
     gene            complement(963..1520)
                     /locus_tag="ACSIGJ_001412"
     CDS             complement(963..1520)
                     /locus_tag="ACSIGJ_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HHQ1330254.1"
                     /translation="MDALKLLTNRRSSKKLKPPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(1596..3230)
                     /locus_tag="ACSIGJ_001413"
     CDS             complement(1596..3230)
                     /locus_tag="ACSIGJ_001413"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HHQ1330255.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELRPFNDT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEALNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEIDKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(3293..3631)
                     /locus_tag="ACSIGJ_001414"
     CDS             complement(3293..3631)
                     /locus_tag="ACSIGJ_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330256.1"
                     /translation="MIFKCRPNAFQTASAVSAFSGGVLEVVRLCCRRLSVLFNPGVML
                     FVPDCPVGGAAGFFQYEMLCPLFRQGRLQTGSNFAYDVFMSAGRLNGGRKPPRRLLVF
                     LPCFAPLPYN"
     gene            complement(3628..4734)
                     /gene="serC"
                     /locus_tag="ACSIGJ_001415"
     CDS             complement(3628..4734)
                     /gene="serC"
                     /locus_tag="ACSIGJ_001415"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HHQ1330257.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLRTAQQEMSDYNGTGFSVMEMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSDGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINDIHPDARSKMNVVFKTASEDLDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            5100..5531
                     /gene="rimP"
                     /locus_tag="ACSIGJ_001416"
     CDS             5100..5531
                     /gene="rimP"
                     /locus_tag="ACSIGJ_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HHQ1330258.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELAAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIGYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIGGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            5559..7061
                     /gene="nusA"
                     /locus_tag="ACSIGJ_001417"
     CDS             5559..7061
                     /gene="nusA"
                     /locus_tag="ACSIGJ_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HHQ1330259.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPDTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIAGGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDRLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMNHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGVDADM
                     LLSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            7073..9904
                     /gene="infB"
                     /locus_tag="ACSIGJ_001418"
     CDS             7073..9904
                     /gene="infB"
                     /locus_tag="ACSIGJ_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HHQ1330260.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVQPEQTAEDAVKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEPKEKAVKPKHERNGKGKDAKKPAKPAAPAVPQPVVSAEEQAQ
                     RDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQAKAAQEAKTGRQRPAKPAE
                     KPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGKGAKGGRDRNNARNGGDERV
                     RGGKKGKKLKLEPNQHAFQAPTEPIVHEVLVPETITVADLAHKMAVKGVEVVKALMKM
                     GMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLGEGAEAVEAEALPRPPVVTV
                     MGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAYHVKTPRGVITFLDTPGHEAFTAM
                     RARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPIVVAVNKIDKDTANPERIRQ
                     ELTQHEVIPDDWGGTVQFIDVSAKKGTNIDALLEAVLLEAEVLELTAPVDAPAKGIIV
                     EARLDKGRGAVATLLVQNGTLKKGDMLLAGTAFGKIRAMVDENGKSITEAGPSIPVEI
                     LGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLAKQQAAKLENMFNNMGETQA
                     QSLSVIIKADVQGSYEALAGSLKKLSADEVKVNVLHSGVGGITESDVNLAIASGAFII
                     GFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMSGMLSPEKKEQVTGTVEIRQ
                     VISVSKVGNIAGCMVTDGVVKRDSHIRLIRNNVVIHTGELASLKRYKDDVKEVRMGFE
                     CGLMLKGYNEIMEGDQLECFDIVEVARTL"
     gene            complement(10064..11404)
                     /locus_tag="ACSIGJ_001419"
     CDS             complement(10064..11404)
                     /locus_tag="ACSIGJ_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HHQ1330261.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADMLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQLPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GGARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAAKLPPELNKARSHWKWSSLNTHRPETSRAERREYAEKIGIRLAGK"
     gene            complement(11478..12818)
                     /locus_tag="ACSIGJ_001420"
     CDS             complement(11478..12818)
                     /locus_tag="ACSIGJ_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HHQ1330262.1"
                     /translation="MLNPSRKLVELVRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRDRMLRDTLERVRAGSFWLWVVAASMMFTAGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLATLFLRVKVGRFFSSPATWFRGKGPVNQAVLRLYADQWRQPSV
                     RWKIGATAHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPS
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIVCYGILPRLLAWVVCKILLKTSE
                     NGLDLEKTYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWALMLETEWQDGQ
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRVAQEGRLKDQ"
     gene            12961..13587
                     /gene="trhA"
                     /locus_tag="ACSIGJ_001421"
     CDS             12961..13587
                     /gene="trhA"
                     /locus_tag="ACSIGJ_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HHQ1330263.1"
                     /translation="MYAGERFNTYSHLSGLILAAAGLMLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYIVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(13652..15070)
                     /locus_tag="ACSIGJ_001422"
     CDS             complement(13652..15070)
                     /locus_tag="ACSIGJ_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HHQ1330264.1"
                     /translation="MENILSVLVGTVNRFLWGYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWLSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIVALGFIGNATQANSIASAVTIAFDVPSLAVGIVFAVLAGMVVIGGVNRIANIA
                     RFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMTAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFAFTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWNLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKQ"
     gene            15397..16125
                     /locus_tag="ACSIGJ_001423"
     CDS             15397..16125
                     /locus_tag="ACSIGJ_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHQ1330265.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIGLRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDELMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(16184..16672)
                     /locus_tag="ACSIGJ_001424"
     CDS             complement(16184..16672)
                     /locus_tag="ACSIGJ_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HHQ1330266.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCAAVVLACKPKGRVGGLSGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
BASE COUNT         4475 a         4853 c         4314 g         3361 t
ORIGIN      
        1 tctcttctct tctcttctct tctcttctct tccgcagcgc aggcggcaag taaagacaat
       61 ggccgcagcc cgtattatgt gcaggcggat ttagcctacg ccgccgaacg cattacccac
      121 gattatccgc aagcaaccgg tgcaaacaac acaagcacag taagcgatta tttcagaaac
      181 atccgtgcgc attccatcca cccccgggtg tcggtcggct acgatttcgg cggctggaga
      241 atagcggcag attatgccag ttatagaaaa tggaacaaca ataaatattc cgtcaacaca
      301 aaagaggtgc aaagaaataa caatggcaac tggcaagaac tgaagacgga aaatcaggaa
      361 aacggcagct tccacgccgc ttcttctctc ggcttatccg ccatttacga tttcaaactc
      421 aacgataaat tcgataaatt caaaccatat atcggtgtgc gcgtcgccta cggacacgtt
      481 aaacatcagg ttcattcggt gagaaaagaa accacgactg ttttcagtaa accaagtggc
      541 actacaaagc caggcgagat cccaagtttg gttaccaaac ctgcctatca cgaaagcaac
      601 agcatcagca gcttgggtct tggtgtcatc gccggtgtcg gtttcgacat cacgcccaag
      661 ctgaccttgg acaccggata ccgttaccac aactggggac gcttggaaaa cacccgattc
      721 aaaacccacg aagtctcatt gggcatgcgc taccgcttct gattccccga caccgatgcc
      781 gtctgaacct tcagacggca tgagaccttt gcctgcgtac ttggtacgct ggtcgcctcc
      841 gaacatggcg cgacacccga catttccgcc gaacgcatcg ggcgtttcat gaatccggtt
      901 taaaacgcat ggaaaaatgc cgtctgaaag cctttcagac ggcattgtgc ttgagattcc
      961 gtttaccaat ggctgacaaa cgcttccaaa tcggtattct tgggcttatg cacttcctct
     1021 gtcggcgtgc cgaccatcat cagcccgatg attttatcct tatccgcaca tccgaaagcc
     1081 tcacgcaaca aggggctgtt gacccacatc cccgttatcc agacattgtc gaaaccttgc
     1141 gccgttgccg ccagttgcag cgcatacgcc gcacaacccg ccgtcagcat ctgctcccat
     1201 tccggtttcg gcttaggcac atcgcggttc ggcgcaaacg ttaccccgat aaccatcggc
     1261 gccatattgc ccactttttc cgcctttttc atcgcatcgt cgccgaaatt caattcggca
     1321 accgtttgct ttaacacatc gcgaaaacgt tgcaatccta cctcgccttg aatcacggta
     1381 aaacggaagg ggcgcatatt gccgtgatcg ggaacttggg ttgccgcctg aaatatttgt
     1441 tccaactccg ccgcatcggg ggcggggggc ttcagctttt tggaagatcg gcggttcgtc
     1501 aataatttta aagcatccat atcgttatac tccggtcatc ggtcgggtgt tcggacacat
     1561 atgccgtccg aaggcttcag acggcatatc cggcatcagc gcggacggcg gcaggctgcc
     1621 aatatatcca tttccttccg ataggtttgg ctgttggaaa tgtccatcag ccccaatacc
     1681 gtgctgaaat agtggtcgtg cgaatattcg ttttccgccg ctttttgttt gaggcattgg
     1741 aaatctatgc ccccgtgttg gcggaaggct ttggaaaacc acataaccat cgggatatgc
     1801 gtctgcccgg aaggcgcgat ggcgtaaggc gcggcgtgca ggtacatccc gttttcgccc
     1861 aaactttcgc cgtggtcgga aacataatgc accacgcttt ccaaatcgtc gcggttttca
     1921 agtttgcgga taaccttgtc gataaactgg tccacataca aaaccgtatt gtcgtaagtg
     1981 ttgaccagcg tggcgcgggt gcatttgtcg atttcgttgg tatcgcatgt cggcgtgaat
     2041 ttgcgctcgg cttcggtgta gcgttcgtaa tacgtcggcc cgtggctgcc gatggtatgc
     2101 aggattaaaa ccgcgtcttt atcgtttttg ttgagggctt cgtcgaactt ggtcaacagg
     2161 atattgtcga ggcactcgcc gttgcggcag tattcgggca ggttgagcga agtaacgtcg
     2221 gtattcggca ctttgccgca cacgcccttg cagccggaat cgttttccaa ccaagtaact
     2281 tccacgccgg cgcgctgcac gatgtccagc aggttgtctt ggtgttcggc tttgatttcg
     2341 tcataatccg tgcggtcgaa ggttgagaac atacacggca gggagtgcgc ggtcgatgtg
     2401 ccgcagcttc tgacctgcgg gaaattgaca atttcatcgc cgcgcgcggc aagcagcggc
     2461 gtagtttggc ggctgtaacc gttcaaaccc cagttggcgg cacgcgtagt ctcgcccacg
     2521 accagcacca cgaaacggcg caggctgccg gccggccggt tttgcacgac cgccatatcc
     2581 aattgcgtat aaggaatatt ggaacgcttc caatctttgt atttcgacac gcccgcgccg
     2641 atgaaattag acggcacaat cagatgggtt actgatttat tgttgcggaa aaacgaggcg
     2701 taatcctgat attgcaacat cgcgatgccc aacgcgcaca aaaaggaaac ggcggcaagc
     2761 acaaggcgcg tcaaaagctc cttataccaa acgcggtatt tgaccttgac ggcgatatat
     2821 gccagcgcgg gcaatacgcc caaacataca atccacagca catagcccgg cgtaatcagg
     2881 cgcgcgcttt ccgccgccgt ggtttgcaag acattattca acatcgactt gttgaaatag
     2941 atattgaaaa atatttcttg gtaagacacc gccgcactga taaccaatat caatggaatc
     3001 aacactttat gcacaaaagg cagggcaatg acgtgaaaaa cgaaattact taaaaaaaac
     3061 agcaccaccg gcatcgtata gaggaagatg tccgccccgg tgtcgttaaa agggcgaagt
     3121 tcgacaactt tggcaaaaaa ggcgtaattc aataccagcg aagaatacag ggaaagaaag
     3181 gcaatcagcg cggaagagcc gagcttcggc ctcaggttcg gttttatcat ttggaatgtg
     3241 tcggataagg gttggaaaag gcatccggaa tccggattat tgaaaaagat tcttaattat
     3301 aaggcaacgg agcaaagcag ggcaagaaaa cgagtaggcg gcgcgggggt ttccgcccgc
     3361 cattcaaacg tccggcagac ataaaaacat cgtaagcaaa attcgaaccg gtctgcaacc
     3421 gcccctgccg gaaaagcggg caaagcattt catattggaa aaacccggcc gcgccgccga
     3481 cgggacagtc cggcacaaac agcatcacgc ccggattgaa aaggacggat agccgccggc
     3541 agcacaatct taccacctcc aaaacgccgc cggagaacgc cgaaacagcc gatgccgtct
     3601 gaaacgcatt cgggcggcat ttgaaaatca accgtaacgc cgttggaaat cgcgcataaa
     3661 atctgccaaa gcccgcacgc cttcaagcgg catcgcatta taaatgctgg cgcgcatccc
     3721 gccgacagac ttatagccct tcaacaggca caagccctgc aattcggctt ccagcacaaa
     3781 acggcggtcc aagtcttcgc ttgcggtttt gaagaccacg ttcattttgg agcgcgcatc
     3841 aggatgaata tcgttgatgt aaaaaccgcc gctgccatct atcgtctcat acaaggtttg
     3901 cgccttcagc cgattgaccg cttcaatttt tttcacaccg ccctgcgcct gcagccagcg
     3961 gaacaccagc cccgacatat aaatcgcgta agttgacggc gtgttgtaca taccgtcgcg
     4021 gttcaaatga gaacggtagt tgaacacatc gggaatatcg ttcggacaac gctcgagcaa
     4081 atcctcacgc acaatcacca ccgtaactcc tgccggcccg atgtttttct gtgcgcctgc
     4141 gtaaatcagt ccgtagtcgg caacatcaaa ctcacgcgac agaatctcgc tggacatatc
     4201 gcacaccaac ggcggcatgc cgtctgaaag gcgcggcact tcacggtatt gcagcccgtt
     4261 gacagtttca ttgacggcaa aatggacaaa cgccgaatcg ggtgcaacat cccacgtttc
     4321 cacaggcggc aggtcaagat agtcgaactg ctcgcccccg tgcgccgcca aacggatttc
     4381 cgtatcggtc aaacggctca tctgttcata ggcgatacgg ctccagttgc ccgttaccac
     4441 cgcgtcggca gtgcggaaac cgtgtgccag attcatggct gccatattaa attgggttgt
     4501 tgctccgccc tgaagaaaca atatcttata gttgtcaggc actttcaaaa gctgcctcaa
     4561 atcctgttcc gcatgatgca ggatgctcaa aaacatttcc gaacggtggc tcatttccat
     4621 cacggaaaaa cccgtaccgt tgtagtccga catttcctgc tgcgccgtcc tcaacacagc
     4681 ttcaggcaac acggcagggc cggcggaaaa attgtaaatc ggataaagag acatgatgca
     4741 gccctggttc tgaacaataa cccgcccgat tttaggcttg cagcaggctt tgtgcaagga
     4801 tgggaaatac ctgccctccg cccgattcca tgccgcccga acacgggaaa taatatcaat
     4861 atattgattt acaaacataa aaatcatgct agcgacaaat agatacattt gttttgtcaa
     4921 caatattcac gatttcccat tacaaacctc ccgtacaccc gcttttttcc gtcccaaaaa
     4981 cacaaaataa atcaacactt tcatttctcc gcaaaagcgg ttataatcac gccgattttt
     5041 caacttttga cgaaaaatgg ccacggggcc atttttttat tatgtacata gggagcagca
     5101 tggatattca aaccatcctc gaaaaaaccc tgcccggcct gggatacgaa ctggtcgatt
     5161 tcgaactggc cgcgcaagga acattgcgcg tgttcatcga caaagaaggc ggcattaccg
     5221 tcgaagactg cgccaccgtc agcaaccact tgagccgcgt cttcatggtt gaagacatcg
     5281 gctacaaaaa cctggaaatt tccagccccg gactcgaccg ccccttgaaa aaagccgccg
     5341 acttcgtgcg ctttgccggt cagaatgcca aaatcaaaac ccgcctgccg ataggcggtc
     5401 agaaaaactt tatcggtaaa atcgaaggct gcgaaaacga cactgttacc gtttccttcg
     5461 acggcaaaac cgtgcaaatc gaattgggca acatcgacaa agcccgcctg cgccccgaat
     5521 tcaaattcta aaacacagca atattggaga tgttcaaaat gagtcgtgaa atgttacagc
     5581 tggcagaagc actggcaagc gaaaaaaacg ttgatgcgga agtcgtcttc caagcactgg
     5641 aattcgcact ctctaccgcc gccaagaaaa aggcagaccg cgaacacatg gacgtgcgcg
     5701 tccaaatcaa ccgcgatacc ggcgaatacc aaaccttccg ccgctggctg attgtggccg
     5761 atgaagacta tacctatccc gatgtcgaaa aaaccatcga ggaaatccaa gaggaaatcc
     5821 ccgatacgac catccaaatc ggcgaatact acgaagagca gctgcccaac gaaggcttcg
     5881 gccgccaagc ggcgcaaact gccaaacaaa tcatcctgca acgcatccgc gatgccgagc
     5941 gcgagcagaa tctgaacgag ttcctcgccg tcaaagaaga catcgtgtcc ggcacggtca
     6001 aacgcgtcga acgccacggc atcatcgtcg aagtcgttgc cggcaaactg gacgcgctga
     6061 ttccgcgcga ccaaatgatt ccgcgcgaaa acttccgcag cggcgaccgc atccgcgccc
     6121 tcttcctgcg cgtcgaagaa atcggcaaca ccggccgcaa acaagtcatc ctgagccgta
     6181 cttccggcga tttcctcgtc aaactgtacg ccaatgaagt acctgaaatt gcaggcggca
     6241 tgcttgaaat ccgcgctgtc gcccgcgacc cgggccaacg tgccaaagtc gccgtcaaag
     6301 ccaacgacca gcgcatcgat ccgcaaggca cctgtatcgg cgttcgcggt tcgcgtgtca
     6361 atgccgtcag caatgaattg tccggcgagc gcatcgacgt tgtcctttgg tcgcccgagc
     6421 ctgcccaatt cgtcatgagc gcgctctcac ccgccgaagt cagccgcatc gtcatcgacg
     6481 aagacaaaca cgccgtcgat gtcatcgttg ccgaggaccg gctcgcgctc gccatcggac
     6541 gcggcggtca aaacgtgcgc cttgcttccg acctgaccgg ctggcagctc aacatcatga
     6601 cttccgccga agcagacgaa cgcaatgcgg cagaagatgc cgccatccgc cgcctgttta
     6661 tgaatcactt gaatgtagat gaagaaaccg ccgacgtact ggttcaagaa ggttttgcaa
     6721 ccttggaaga agtcgcctat gttcctgccg ccgaactgct tgccattgaa ggatttgacg
     6781 aagaaatcgt cgatatgctc cgcaaccgtg cccgcgatgc catcctgacc atggcgattg
     6841 ctgccgaaga aaaactgggc gaagtgtccg acgatatgcg taacctcgaa ggcgtagatg
     6901 ccgatatgct cctcagcctt gccgaagcag gcattaccac ccgcgacgac ttggcagagc
     6961 ttgccgtgga cgaactgatt gaaatcaccg gtgtaaacga agaaactgca aaagccgtca
     7021 tcctgaccgc acgcgaacac tggtttaccg aagacaaata aagggggtac agatgagtaa
     7081 cacaaccgta gaacaatttg ccgccgagct gaaacgcccc gtcgaagacc tgttgaaaca
     7141 gttgaaagaa gccggcgtca gcaaaaacag cggcagcgat tccctgacgc tggacgacaa
     7201 acagcttctg aacgcctacc tgaccaagaa aaacggcagc aacggcggca ccatcagcat
     7261 ccgccgcacc aaaaccgaag tcagcaccgt tgacggcgta aaagtcgaaa cacgcaaacg
     7321 cggacgcact gtcaacattc cttctgccga agaattggca gcacaagtaa aagccgccca
     7381 aacccaagcc gcacctgttc agccggagca gacggcagaa gacgcggtaa aagcccgagc
     7441 cgaagctgcc gcacgcgcag aagcccgtgc caaggcagaa gcggaagcgg caaaactgaa
     7501 agcggcaaaa gcaggcaaca aagccaaacc tgccgcgcag aaacctaccg aagcaaaagc
     7561 cgaaaccgca cccgttgcgg cggaaaccaa acccgccgag cccaaagaaa aagccgtcaa
     7621 gccgaaacac gagcgaaacg gcaaaggcaa agatgccaaa aaaccggcga aacctgccgc
     7681 acctgccgtg ccgcaacccg tggtcagcgc ggaagaacag gcgcaacgcg acgaagaggc
     7741 gcgccgcgcc gccgcattgc gcgcccacca ggaagccctg ttgaaagaga aacaggaacg
     7801 ccaggcacgt cgcgaagcca tgaaacaaca ggcagaacaa caggcaaaag ccgcacagga
     7861 agccaaaacc ggcagacagc gtcccgccaa acctgccgag aaaccgcagg cagccgcacc
     7921 ggccgtcgaa aacaaacctg tcaatccggc aaaagcgaaa aaagaagacc gccgcaaccg
     7981 cgatgacgaa ggtcaaggcc gcaacgccaa aggcaaaggt gcaaaaggcg ggcgtgaccg
     8041 caacaatgcc cgcaacggcg gcgacgagcg cgtacgcggc ggcaagaaag gcaaaaaact
     8101 caaactcgag ccgaaccaac acgccttcca agcaccgacc gaacccatcg tacacgaggt
     8161 tttggttccc gaaaccatta ccgttgccga tttggcgcac aaaatggcgg tcaaaggcgt
     8221 ggaagtggtc aaagccctga tgaagatggg catgatggtt accatcaacc aatccatcga
     8281 ccaagacacc gccctgattg tcgtcgaaga actcggacac atcggcaaac ccgccgccgc
     8341 cgacgatccc gaagccttct tgggcgaggg cgcggaagcg gtggaagccg aagcattgcc
     8401 gcgtccgccc gtcgttaccg tcatgggcca cgtcgaccac ggcaaaacct cgctgctgga
     8461 ctacatccgc cgcgccaaag tggtacaggg cgaagcgggc ggcattacgc agcacatcgg
     8521 cgcgtaccac gtcaaaaccc cgcgcggcgt gattaccttc ttggacaccc cgggccacga
     8581 agcctttacc gctatgcgcg cgcgcggcgc gaaagcaacc gatatcgtga ttcttgtggt
     8641 cgccgccgac gacggcgtga tgccgcaaac catcgaggca attgcccacg ctaaagcggc
     8701 aggcgttccg attgtggttg ccgtcaataa aatcgataaa gacactgcca accccgaacg
     8761 catccgtcag gaactgaccc aacacgaagt catccccgac gattggggcg gcacggttca
     8821 attcatcgac gtttccgcga aaaaaggaac gaacatcgac gcgctgctcg aagccgtatt
     8881 gctcgaagcc gaagtattgg aactgaccgc acctgtcgat gcacccgcca aaggcatcat
     8941 cgtcgaggcg cgtttggaca aagggcgcgg cgcggttgcc acattgctgg ttcaaaacgg
     9001 tacgctgaaa aaaggcgaca tgctgctggc cggtacggca ttcggcaaaa tccgcgcgat
     9061 ggtcgatgaa aacggcaaat ccattaccga agccggcccg tccatccccg tcgaaatcct
     9121 cggcttgtcc gacgtaccga atgcgggtga agacgcgatg gtattggcgg acgagaaaaa
     9181 agcgcgcgaa atcgccctct tccgccaagg caaataccgc gacgtgcgcc ttgccaaaca
     9241 gcaggcggcg aagctggaaa atatgttcaa caatatgggc gaaacccagg cccaatcttt
     9301 gtcggtcatc atcaaggcag acgttcaggg ctcttacgag gctttggcgg gcagcctgaa
     9361 aaaactgtcc gccgacgagg tgaaagtgaa cgtgttgcac agcggcgtgg gcggcattac
     9421 cgaatcggat gtcaaccttg ccatcgcttc gggcgcgttc attatcggct ttaacgtgcg
     9481 tgcagatgcc tcttcgcgca aacttgccga aaatgaaaac gtggaaatcc gctactacaa
     9541 catcatctac gatgccatcg acgacgtgaa ggcggcgatg agcggcatgc tttctcccga
     9601 aaagaaagaa caggttaccg gtacggtcga aatccgtcag gtcatctccg tttccaaggt
     9661 cggcaacatt gcaggctgta tggtcaccga cggcgtggtc aaacgcgatt cccatatccg
     9721 cctcatccgc aacaacgtgg tcatccacac gggcgaactg gcttcgttga aacgctataa
     9781 agacgacgta aaagaagtcc gcatgggctt cgagtgcggt ctgatgctca aaggctacaa
     9841 cgaaatcatg gaaggcgacc aactggaatg cttcgacatc gtcgaagttg cccgcaccct
     9901 gtaattcctt tgcaaataaa atgccgtctg aagcgttcag acggcaacga aacgggttct
     9961 gtatcataca gaacccgttt tttgtcgcaa atcggcttca gcgaccctcc tgccttatcc
    10021 cgatttgaat ctgacttgcc atacaaacag gcttcagacg gcattatttg cccgccaaac
    10081 gtatcccgat cttctccgca tattccctgc gttcggcgcg gctggtttcc gggcggtgcg
    10141 tattgagcga cgaccatttc caatgactgc gggctttgtt gagttcgggc gggagtttgg
    10201 cggcatccca cggggctttg cggctgtgca gctcgatatc cgactgtgcc gcgtgtccgc
    10261 gcgtttgcag gacgtggagc agatcgaggg cgcgggcggc gagcagggtc agggtttcag
    10321 ggtcggtgtg cagggtttga cggccggcga gtttgtcgga aatggtgcgg gtattgggca
    10381 ggatgccgcc caaaaagccg ccgattgccg tacccaatcc gagcgagccg ccgagtgtgg
    10441 cgatgtccag ccccaagccg atgagcgcgc cggttgccgc gcccgtgccg gtgcggatgc
    10501 cgtattgttt gagcaattcg ctgtcgaacg ggtcttggcg gaaggcttgc ggcatccagt
    10561 cgccgccgtc gatttcgctg tggtagaaac ggtaaagggc aaacagccgc tgctgcatct
    10621 gccgttcgag ttggcgtatt tccgcctgca tggtttgcag cacggtggcg gtatcctcgt
    10681 tttcgtccac ttcctgcctg aaggcggcgg catcgagcag gaaatcggcg atttcgcggc
    10741 gcgctccgcc gtccagccgc tgccattcgc gccggcgcat ggctgtcagg cggtcaagtg
    10801 tgctgcgttc gggcaacatg gtggcgaggt tttcccacag gcgcaattcg ccttcaaaat
    10861 caaaggcgac ggtgtcgaac cctgcgaaaa cgtgcaggtt tctcctcgcc agcatggttg
    10921 tccacgattc gggaagctgt ccgccggtaa agttgaacac gggcataacc ggtttggcac
    10981 accatgaaag gatggtcaat tcatcccgat atttgtcgag gacgggttcg cgcgcgtcga
    11041 tgacgtacat tgccatatcg ctttgcaaaa cctgccgcag cactttggct tcttggttga
    11101 aatcgtgatg cgcgccgtgg ctgccgagaa actgttgcag ccgctcgatg ccgtctgaac
    11161 gattgtccgt atggttttcc agccattcca aaacaccgcc cgcgtcttcg agtccgggcg
    11221 tgtcatacag gaaaaccaac atgtccgcgc cgtcgctgat ggcggcttct tcgacatggc
    11281 gtgtggtgga cggtgcgttt ctgacttcgc cgaagccgct gtcgcgcaac agggtgcgca
    11341 ggagcgaggt tttgccggtg ttggtgtgtc ccacgacggc gagggaaagg ggttgtttgt
    11401 tcatgatgtt tttgaagaat ggattttcag acggtctttt ttcagaatgg cggcttaaca
    11461 gaacatttca agtgagttta ttggtctttc aaacggcctt cctgcgccac cctgtcaggc
    11521 tcaagccacg ccgcgccgca ttcggtcagc gcgttacgcc aatgttccag cttttccgaa
    11581 aggtcgtctg aaagcccctg ttccgccaaa agctgcacca ccgcgccgcc ctgcgccgct
    11641 tccgaaagcc gcacaatctg ccgcagcacg ccccggtccg gcacagtttg ggcgcgtacg
    11701 ccgataagca gttgcgccgg tttctgcttc agctctgtct ccagcgcggc aacctgttcc
    11761 cgattggcgg caacgccctt atccagccat tcctgcgcca gcctgccctc gaaccattgg
    11821 ccgtcctgcc actcggtctc cagcatgagc gcccatttcg gcgcatcgtt caagacgatt
    11881 ttcggcgaaa cggcggacac ggtttcccga cgcgtatccg catcggtgat tttgttctgc
    11941 cagcggcgga tgaccgcctg ataataggtt ttttccaaat ccaatccgtt ttcgcttgtt
    12001 ttcaaaagga ttttacacac tacccaagcc aagaggcgcg gcaggatgcc gtagcagacg
    12061 atactgccga ccagcagccc cgaccaagcc cgcgcatcgg caatattgcc gttcagacga
    12121 ccttcgatga ccgcccgcgc atcggggaca gggaaaccga gtttcgacgg cagccatgcc
    12181 aacatttcca ccgcgcgtac cgaagcggca ttgctcaaca gcgtgctttc ccagttgaac
    12241 gtatattgcc gcaccaaaag cagcagcaat accgacacca gcattccgag cagcgtgcag
    12301 agccacaagc tgtgcgccgt tgcgcctatt ttccatcgta ccgaaggttg ccgccactgg
    12361 tccgcataca gccgcaacac cgcctgattt acagggcctt tgccccgaaa ccacgtcgcc
    12421 ggactgctga aaaaccgccc cactttcacg cgcaggaaca acgttgccaa ccatactgcc
    12481 agcatcagcg tattcatgcc caacactccc gccaaaacta aaaagaaatt cagcccctga
    12541 ttgtccatca gaagataagt gcctgaaaat ccggcggtaa acatcatcga tgccgccacc
    12601 acccataacc agaacgaccc cgcacgcaca cgttccaacg tgtcccgcag catacggtcc
    12661 ctgtcgatca tctccgcccg acggatgatt ttttcctccg tactgccgtc cacgcggcgc
    12721 aaagcctccg tcgcctgcac gggatcgccg ctgaaaataa aaccgccttc atccaaaata
    12781 cggaccagct caaccagttt tcgggatgga ttcaacataa aatgccgtct gaaaataaaa
    12841 aacaaatttt aacacacgca ttttcaagaa tattcacagt gtaggcaaag agtaaatccc
    12901 acacagaggc aaaagtatcg gcgtaaactg actgcctcta ctttcccgaa agattgtgcg
    12961 atgtatgcag gcgaacgctt caatacttac agccatttga gcggtttgat tctggcggcg
    13021 gcaggtttga tgctgatgct gctgaaaacc ataggacacg gggacggcta ccgtatcttc
    13081 agcgtatcgg tttacggcat cagccttctt ctgctctatt tgagttcctc gctgtaccac
    13141 ggaattgcag ccggaaaact gaaaagcatt ttgaaaaaaa ccgaccactg catgatttat
    13201 gtgctgattg ccggaagcta cacaccgttt gcactggttt ctttgagaaa cgggccgggc
    13261 tggacggtat tttcactgtc ctggctgctg gcggctgcag gaatcgcaca agaactcacc
    13321 atcggacgga aaagcgaaaa acgtctgctg tctattgcga tttatatcgt aatgggctgg
    13381 atggtcttgg cggtaatgaa atccctgaca gcctcactcc cgccggcagg actggcttgg
    13441 ctggcggcag gcggtatgct gtacagcgtc ggcatttact ggtttgtaaa cgatgaaaaa
    13501 atccgacacg ggcacggaat ctggcatctg ttcgtattgg gcggcagcat aacccaattt
    13561 gtcagcgtgt acggttatgt aatctgaatg ccgtctgaaa cacaaaaacc tcccaaacct
    13621 gaaggcaggg aggttttctc tttgccgggc attattgttt atcgtggaac tcgtggaatt
    13681 catactgata ggacaaatcc cgacccgctt ttttctgtgc caaataatca tcataaatgg
    13741 cgcggatttc cttacgcagc aagaacaggg caatcaggtt ggggataacc ataaagccgt
    13801 tgaacatatc ggacagattc caaaccaaat cgactttgcc gagcgtaccc aaaacgatag
    13861 caagcagaac caatgcgcga tagatgccca agtgtcttcc cctgaaaaga aaacggatgt
    13921 tggactcgcc gaaataatac cagccgataa tggtcgtaaa ggcgaagaag gtcagacaca
    13981 cggcaagcaa ttgcgaaccg aagcccggaa atgccttgtt aaaggcaaat tgagtaaccg
    14041 ccgcgccctg ttcgcccgaa aggttggcat cggtcagcag gataatcaat gccgtagccg
    14101 tacataccaa aatcgtatcg ataaatacac cgacaaatgc cgtcatacct tgctgaacag
    14161 gatgcttcac atccgcagtc gcgtgggcgt gcggagtcga acccatacct gcttcgttgg
    14221 aaaacagacc gcgcgccacg ccgaaacgta tcgcttcacg cataccgata cccgcagcac
    14281 cgcccaaaac ggcctcggga ttgaaggcgg cggtaaagat gtggttgaac atcggcacaa
    14341 tatggtcgga aaattcaaac aggataacga cggcgcacaa aatataaaca accgccataa
    14401 acggcacgac aaaccgggca atattggcaa tacggttcac gccgccaatc acaaccatgc
    14461 ccgcaaggac ggcaaacaca ataccgactg ccaaagaagg cacatcaaat gcaatggtaa
    14521 cggcagaagc gatggaattt gcctgagtcg cattgccgat aaagcccaac gcgacaatca
    14581 gcgcgatgga gaaaaagccc gacaggaaac gcgccgcgcc cctgccgatt ttcggagtca
    14641 gaccgtgcgt aatataaaac gccggcccgc cgatgtattt gccgtggctg acgacgcggt
    14701 atttctgcgc cagcagtgct tccgcaaaaa tcgtggacat ccccaaaacg gcagaaagcc
    14761 acatccaaaa aatcgcaccc ggcccgcctg cggtaatggc ggtcgccaca ccggcgacgt
    14821 tgcccgtacc gatttgcgcg gatatagcaa ccgccaacgc ctgaaactgc gataaggact
    14881 tatcgtcttt atcacctttg gcaaacaagc cgccgaatac ggatttgaat cccgcgccca
    14941 gcttggtaat ctgcggcgca ccaagataca gcgtaaaaaa caggccgata cccaaaagcg
    15001 cataaatcag caggtagccc caaagaaacc gattgactgt acccaccaga acagacaata
    15061 tattttccat aaaataaacc ttatcttacg attaaaatga ctgttttcca aaagacattc
    15121 caataaggaa acacggcgag cagaccgtat ttgccgcaac agatgcctta aattgtcaat
    15181 aatccgagag aagctgtgcc agcataccgt aaaatatcgt taattaaaat atttctttat
    15241 ttttaagcgg aaagcggagg aaatcgtttt cagacggcat cgacaacggc acggcataaa
    15301 acaggatatt ttgggtactt gcaacttatg ttaaaatgcc gaccgtaaaa aatctgacaa
    15361 aaacagatta attatttgaa ataagaaagg aaatttatgg caggccatag caagtgggcg
    15421 aatatccagc ataaaaaagc ccgtcaggat gccaaacgcg gcaaaatctt cacccgttta
    15481 atcaaagaaa tcaccgttgc ggcgcgtatg ggcggcggcg atcccggcgc aaatccgcgc
    15541 ctgcgtctgg ctttggaaaa agcagccgaa aacaatatgc ccaaagacaa tgtgcaacgc
    15601 gccatcgaca aaggtacggg taacttggaa ggcgtggaat acatcgggtt gcgctacgaa
    15661 ggctacggca tcggcggcgc agctttgatg gtggactgcc tgaccgacaa caaaacccgc
    15721 accgttgcgg acgtacgcca cgcatttacc aaaaacggcg gcaacttggg taccgacggc
    15781 tgcgtggcgt tcaacttcgt gcatcagggc tatttggtat tcgaacccgg cgttgacgaa
    15841 gacgagctga tggaagcggc tttggaagcc ggtgcggaag acgtggttac caacgacgac
    15901 ggttccatcg aagtcattac cgcgccaaat gattgggcgg gcgtaaaatc cgctttggag
    15961 gcggcaggtt acaaatccgt tgacggcgac gttacgatgc gcgcccaaaa cgaaaccgaa
    16021 ctctccggcg acgatgccgt caaaatgcaa aaactgattg acgcgctgga agacttggac
    16081 gacgtgcaag acgtttacac ttccgccgta ttgaatctgg actgatacgc agcacagcag
    16141 acatacaaca aaatgccgtc tgaacctttc agacggcatt gatttattta gcccttgccc
    16201 acgcccacca aaccgtcagg acaaccgcca aaaaatacgc cgcgctccaa acaggcaaag
    16261 caacccctaa cagataatcc ggttcggcac aatttccgaa cccgcgcaca acaggctcga
    16321 accaatcaaa caaaggccag cccttcaatc gaaacgtcca cggcgcgccg cacgaaggag
    16381 ccgtacccgg cggcagcgac tgcaaccaca actgatatgc cgcaacagaa atacccgtaa
    16441 cggcgggaat actgataaac actgcgcctg acagtccgcc caccctgccc ttgggcttac
    16501 acgccagcac aactgccgca cacaatgcgg ttgccaaaac acataaccgc tgactgatgc
    16561 acaaaacgca aggctccata cccaaaacat actgtgccgc caaagaaccg gcaaatgcac
    16621 agaccgaaac ggcaaacagc agccaaacgg cttttctaaa taacggggtc attttctcaa
    16681 cacaccaatc aaaataccga tatgccgatt ttgctggata tatcccgaga tggcaaggga
    16741 cgaatcgaca ccggcacagg tgcggacgat ttcggttttc ccgatatgca caaaacctga
    16801 caaaggacaa aacggcgatt tgtccgcaca atgcccgaaa aaatacatcc ggaggatttg
    16861 aatttgaatt tcggcaaatt tagcgaatca aaagcaacag ccgataaaaa aatcaaaagc
    16921 ttatttcaat gtttcaacac acaggacgac acataaagca ccgccctatg tgccgtcctg
    16981 atttggaagg ggttacaccc ctc
//
LOCUS       DBJGGI010000044        16979 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.044, whole genome shotgun sequence.
ACCESSION   DBJGGI010000044 DBJGGI010000000
VERSION     DBJGGI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16979
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.044"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..117
                     /locus_tag="ACSIGJ_001425"
     CDS             <1..117
                     /locus_tag="ACSIGJ_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HHQ1330267.1"
                     /translation="EHDDGRPHYDVEIVKNGQEYKVVVDARTGRVISSRRDD"
     gene            199..783
                     /gene="ruvA"
                     /locus_tag="ACSIGJ_001426"
     CDS             199..783
                     /gene="ruvA"
                     /locus_tag="ACSIGJ_001426"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HHQ1330268.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAASPAADETEDIVSTLLA
                     LGYNEREAKAAVKGVPKGTDVGEGVRLALKNLLK"
     gene            783..920
                     /locus_tag="ACSIGJ_001427"
     CDS             783..920
                     /locus_tag="ACSIGJ_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330269.1"
                     /translation="MPSEGGAAFALTKPCVSAPRCLPDGFSRKMALLSLLSDGIVFDA
                     V"
     gene            946..2802
                     /locus_tag="ACSIGJ_001428"
     CDS             946..2802
                     /locus_tag="ACSIGJ_001428"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHQ1330270.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLCRFVWSSMDGVRKWIA
                     ALAALTAGIGIMEALIFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMAFSVVWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIIGFASVMRLLIPRLGQTAARQAN
                     ARSLMTGRITDAYSNIATVKLFFHGAREAVYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSAAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGI
                     VNDGMATLSKPHTILDKPQALPLNVPQGAIKFEHVDFCYEAGKPLLNGFNLNIKPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRSGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMEGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLSEHVEWQHD"
     gene            2807..3730
                     /gene="rsgA"
                     /locus_tag="ACSIGJ_001429"
     CDS             2807..3730
                     /gene="rsgA"
                     /locus_tag="ACSIGJ_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HHQ1330271.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITAGYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWLEKLKFYETLGYPVI
                     ETRVLENADSLRPVLQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPHYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETGAASPERLAFLQGITDELLG"
     gene            3756..4067
                     /locus_tag="ACSIGJ_001430"
     CDS             3756..4067
                     /locus_tag="ACSIGJ_001430"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HHQ1330272.1"
                     /translation="MPSEAGFGFQTASVLQKCYNPLFTGTPETMKKNTPKSFEEALSR
                     LESLTQSMQGEMPLEDALAAYQEGNELVRYCQTKLAQVEQKLQVLDADGTKELNLESD
                     E"
     gene            4051..4947
                     /locus_tag="ACSIGJ_001431"
     CDS             4051..4947
                     /locus_tag="ACSIGJ_001431"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HHQ1330273.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSGNEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGGAVADAVGQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLERMHSLKTGALIRAAVLLGATACPDLSDAELAVLDAYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(5064..5363)
                     /locus_tag="ACSIGJ_001432"
     CDS             complement(5064..5363)
                     /locus_tag="ACSIGJ_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HHQ1330274.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHLIELA
                     AGFALTASLAYILESRAGAVHNQGWEFYATVVCLYLIFAFPCFVRRYFWHTRNRE"
     gene            complement(5475..6920)
                     /gene="nuoN"
                     /locus_tag="ACSIGJ_001433"
     CDS             complement(5475..6920)
                     /gene="nuoN"
                     /locus_tag="ACSIGJ_001433"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HHQ1330275.1"
                     /translation="MNWSDLNLMPALPEVALLSLLVLLLPADLWASDDKCRWTHYGAL
                     ATVAVTAAVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFVLFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWMPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHTGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLVGAFYYLRVVKVMYFDESGRARPAAGGNNAAKS
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            complement(6930..8426)
                     /locus_tag="ACSIGJ_001434"
     CDS             complement(6930..8426)
                     /locus_tag="ACSIGJ_001434"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HHQ1330276.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLTGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AVIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVVMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFAILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            complement(8540..9703)
                     /locus_tag="ACSIGJ_001435"
     CDS             complement(8540..9703)
                     /locus_tag="ACSIGJ_001435"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease PLD domain-containing
                     protein"
                     /protein_id="HHQ1330277.1"
                     /translation="MTQLFLHSDTPNEKELISIYEKAVSEAVELYIFSAYLTHWDVKN
                     KLNSKLQEFKFIFGQDFGLSKKEAIRKVLKWLPSHLKFTLMVAQGIQGFHPKAVFWKN
                     DKNEYYALIGSSNLTHAAFNSNYEANILTKISEQDFIKVKSWADEIAMKSIPVSEDWL
                     EEYQEAEINYKKSTVRQSVMDKLFMEMPNYNQELIAARRKQMRNHQTVCNQLKNLIKQ
                     CAAGKIDNNDFYGEFNKLWSWKSENKGEGVGNRFQDKTWKRTGKSSDFRKLCIAIQSV
                     FDAPLTERDNVVAKQIDWLKECGVSTRGSVFSEMLCQEYPDRYPVLNAPIKKFLEENK
                     FKSAKGASEGSKYIDLSMKLRALLAMQSEIKDLAELDVLVQAEYRNRTDIDWE"
     gene            complement(9887..11911)
                     /gene="nuoL"
                     /locus_tag="ACSIGJ_001436"
     CDS             complement(9887..11911)
                     /gene="nuoL"
                     /locus_tag="ACSIGJ_001436"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HHQ1330278.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPGATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAVKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHSDGHGEEHHGLGKNDNPHESPLVVTLPLILLAIPSVIIGYIAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLATAGVLSAWL
                     LYVKLPHLPAKIAQAFRPVYVLFENKYYLDALYFNVFAKGTRALGNFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKVQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            complement(11914..12219)
                     /gene="nuoK"
                     /locus_tag="ACSIGJ_001437"
     CDS             complement(11914..12219)
                     /gene="nuoK"
                     /locus_tag="ACSIGJ_001437"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HHQ1330279.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            complement(12216..12887)
                     /locus_tag="ACSIGJ_001438"
     CDS             complement(12216..12887)
                     /locus_tag="ACSIGJ_001438"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HHQ1330280.1"
                     /translation="MTFSVILFYILAAIVLYGAVRTVTAKNPVHAALHLVLTFCVSAM
                     IWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTTNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKTE
                     GEGKA"
     gene            complement(12899..13378)
                     /gene="nuoI"
                     /locus_tag="ACSIGJ_001439"
     CDS             complement(12899..13378)
                     /gene="nuoI"
                     /locus_tag="ACSIGJ_001439"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HHQ1330281.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRCYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            complement(13459..14535)
                     /gene="nuoH"
                     /locus_tag="ACSIGJ_001440"
     CDS             complement(13459..14535)
                     /gene="nuoH"
                     /locus_tag="ACSIGJ_001440"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HHQ1330282.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPRGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGIVGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            complement(14538..16799)
                     /gene="nuoG"
                     /locus_tag="ACSIGJ_001441"
     CDS             complement(14538..16799)
                     /gene="nuoG"
                     /locus_tag="ACSIGJ_001441"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HHQ1330283.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKAVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGVWANPMNTVEELYLAKKFAD
                     GLGVKNFATRLRQQDKRLSDGLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELFMPLLSQEAAHPDEWAGRLKNLSADAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     AEMANTPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPLWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWAGEGVQTASDRLVRVGGVGIYHTDSIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMGTIELAGA"
BASE COUNT         4267 a         4780 c         4332 g         3600 t
ORIGIN      
        1 gaacacgacg acggccgtcc gcactatgat gtcgaaatcg tcaaaaacgg acaggaatac
       61 aaagtcgttg tcgatgcccg taccggccgc gtgatttcct cccgccgcga cgactgaatt
      121 tgatacaatc cgtgccgtct ggagcccgaa ccggtttcag acggcatttt gcacccgaca
      181 cttcaggatt cggcacacat gatcagcaga ctgaccggca aactggttga aaaaaaccct
      241 ccgcaaatcg tcatcgatgt caacggtgtc ggttatgaag ccgacgtatc gatgcagacc
      301 ttctacaacc tgccgcccgt gggtgaaagc gtacaactgt ttacccagct tatcattcgg
      361 gaagacgcac atcttttatt tggttttgcc actgcggaag aacgcaagac cttccgccaa
      421 ctgattaagg tcggcggcat cggcgcgaaa acggctttgg gcattttgtc ggcgatgacg
      481 gcagacgagc tggcgcgggc ggttgcagaa gaagatgtca aacgcctctc ctccgctccg
      541 ggaatcggca aaaaaaccgc cgaacgtatg gtcttggaac tgcgcggcaa gctggtcgcg
      601 catacggtaa cggacgggct gtttgccgcc tcacccgccg ccgacgaaac ggaagacatc
      661 gtcagcacgc tgcttgcgct gggttacaac gaacgcgagg caaaagcggc ggtcaaaggc
      721 gttccgaagg ggacggacgt gggcgaaggc gtgcgccttg ccctgaaaaa cctgctgaaa
      781 taatgccgtc cgaaggcggc gcggcgtttg ccctgacgaa accctgcgtt tccgccccgc
      841 gctgtcttcc cgacggcttt tcccgtaaaa tggcgttatt gtccctcctt tcggacggca
      901 ttgtgtttga tgccgtctga aactgtttta ccgaaatcga aaacaatgtt gaacaaaata
      961 ttttcctggt tcgagtcccg aatcgacccc taccccgaag ccgcgccgaa aacgcccgaa
     1021 aaaggcttgt gccggtttgt ctggagcagt atggacgggg tgcggaaatg gatagccgcc
     1081 ctagctgcgc tgaccgccgg catcggcatt atggaagccc tgatttttca atttatgggc
     1141 aaaatcgtag agtggctcgg caaatacgcg cccgccgaac tgtttgccga aaaaggttgg
     1201 gaactggcgg caatggcggc gatgatggcg ttttcggtcg tgtgggcgtt tgccgcgtcc
     1261 aacgtgcgcc tgcaaaccct tcagggcgtg ttccctatgc gcctgcgctg gaacttccac
     1321 cgcctgatgc tgaaccagag cctcggtttt tatcaggacg aatttgccgg ccgcgtgtcc
     1381 gccaaagtca tgcagaccgc gctggcgttg cgcgacgcgg tgatgacggt tgccgatatg
     1441 gtcgtttatg tgtcggtgta tttcatcact tcgggcgtga ttctcgcctc gctcgactca
     1501 tggctgctgc tgccctttat cggctggata atcggtttcg cttcggtaat gcgcctgctg
     1561 attcccagat tggggcaaac cgccgcacgg caggcgaatg cccgctcgct gatgaccggc
     1621 cgcattaccg atgcctattc caatatcgcc accgtcaaac tctttttcca cggcgcgcgc
     1681 gaagccgtct atgccaagca gtcgatggaa gaatttatgg ttacggtgcg cgcccaaatg
     1741 cggctggcga ctctgctgca ttcgtgcagc ttcatcgtca acacttcgct gaccctctcc
     1801 gccgccgcac tgggcatctg gctttggcac aacggacagg tcggcgtggg tgcggtcgcc
     1861 accgccaccg cgatggcgtt gcgcgtcaac gggctgtcgc aatacattat gtgggaatcc
     1921 gcgcggctgt ttgaaaacat cggcatcgtc aacgacggca tggcgaccct gtccaaaccg
     1981 cacaccatcc tcgacaagcc ccaagccctg ccgctgaacg tgccgcaagg cgcaatcaag
     2041 ttcgaacacg tcgatttttg ctacgaagcc ggcaaaccgc tgctcaacgg ctttaacctg
     2101 aatatcaaac ccggcgaaaa agtcggcttg atcggacgca gcggcgcggg caaatccacc
     2161 atcgtcaacc tgcttttgcg tttctacgaa ccgcaaagcg gcacggtttc gatcgacggg
     2221 caggacataa gcggcgttac ccaagaatct ttacgcgccc aaatcggttt ggtcacacaa
     2281 gatacctcgc tgctgcaccg ttccgtgcgc gacaacatta tttacggccg ccccgacgcg
     2341 accgatgccg aaatggtttc cgccgccgaa cgcgccgaag ccgccggctt catccccgac
     2401 ctttccgatg ccaaagggcg gagcggctac gacgcgcacg tcggcgaacg cggcgtgaaa
     2461 ctctccggcg gacaacgcca gcgcatcgcc atcgcccgcg tgatgctcaa agacgcaccc
     2521 atcctgctgc ttgacgaagc caccagcgcg ctcgattccg aagttgaagc cgccatccaa
     2581 gaaagcctcg acaaaatgat ggaaggcaaa accgtcatcg ccatcgccca ccgcctctcc
     2641 accattgccg cgatggacag gctcgtcgtc ctcgacaaag gccgcatcat cgaagaaggc
     2701 acacacgccg aactcctcga aaaacgcggg ctttacgcca aactctgggc gcaccagagc
     2761 ggcggcttcc tcagcgaaca cgtcgagtgg cagcacgact gaaccgatgc cgtccgaaca
     2821 ccccttttca gacggcattt ccacacccaa ccccaaagaa accatgaacg acaccgccca
     2881 aattaccgcc ggctacggcc gccgctacat cgtccgcacg cccgacggca caacctacga
     2941 agccagcacc cgcaaaaaac gcgtcgattt cgcctgcggc gaccgcgtcc gcatcagccc
     3001 cgtcaacgcc gaacaagttg tgattgaaga ttttttaccg cgccaaagcc tgctctaccg
     3061 ccaagacgcg tggaaaacca aactcatcgc cgccaacgtt acccaactcc tcatcgtaac
     3121 cgccgccgtc ccgagtccga gcgtgcggct gctacagcgc gccctgcttg ccgccgaagc
     3181 cgccggcatc cgcgccgtca tcgtcctgaa caaagccgac ctgcccgaaa ccgccctctg
     3241 gctcgaaaaa ctcaaattct acgaaacgct gggttacccc gtcatcgaaa cccgcgtgct
     3301 ggaaaacgcc gacagcctgc gccccgtcct gcaagggcac agcaacatcc tgctcggaca
     3361 aagcggtatg ggcaaatcca ccctgaccaa cgccctttta ggcagccaaa ccgcccgcac
     3421 cggcgacatt tccgccgcac tcgactcggg aaaacacacc accacccacg cccggcttta
     3481 tgatttgaac ggcgaaaccc aactcatcga ctccccgggt ttgcaagaat ttggtttaca
     3541 ccacctccaa gccgccgatt tgccgcacta tttccccgat ttccgccacc ttgtcgggca
     3601 atgccgcttc cacaactgca cccaccgcgc cgaacccggc tgcgccttca aagccgccgc
     3661 cgaaaccggg gcggcaagcc ccgaacgcct cgcctttttg cagggcatca ccgacgaact
     3721 gctcgggtaa cgccttgccg accggggcgg aaaaaatgcc gtctgaagcc ggattcgggt
     3781 ttcagacggc atccgttttg caaaaatgct acaatccgct ttttaccgga acacccgaaa
     3841 ctatgaagaa aaacaccccg aaatcgtttg aagaggcctt gtcgcgcctt gaatcgctga
     3901 cgcagtccat gcagggcgaa atgcccttgg aagacgcgct tgccgcctat caggaaggca
     3961 acgagctggt caggtactgc caaaccaagc tggcccaagt cgaacagaaa ttacaggttt
     4021 tggatgcgga cgggacgaag gagttgaacc ttgaatccga cgaatgattt gaaagcgtgg
     4081 caacagaggg cgcaggcgca gacagagctg ctgctggaac gctttttgcc gtctggaaac
     4141 gaaatcccgc acacgctgca cgaagcgatg cgctatgcgg ctttggacgg cggcaagcgt
     4201 ctgcgcccga tgctggtgct ggcggcttcg gaattgggcg gggccgtggc ggatgcggtc
     4261 ggacaggcaa tggcggcaat cgaaatgatc cacgtctatt ctttggttca cgacgatatg
     4321 ccggcgatgg acaacgacag cctgcggcgc ggcaaaccga cctgccacat caaatatggc
     4381 gaagccaccg cccttctgac cggcgacgct ttacaaaccc aagccttcga cgtgttgagc
     4441 cgtccgacag aactgcccgc cgcacgccag ttggcaatgt tgtcggtgtt ggcaaaagcg
     4501 ggcggcagcg cgggtatggc gggcggacag gcaatcgatt tggcaaatgt cggcaaacaa
     4561 atggttcaag ccgatttgga acggatgcac agcctgaaaa cgggggcgtt gatccgtgcg
     4621 gcggttttat tgggggcgac ggcgtgtccc gatctgtccg acgcggaact tgccgtattg
     4681 gacgcttacg cggcaaaact gggtttggcg ttccaagtca tcgacgatgt gttggattgt
     4741 gaagcggaca cggcgacttt gggcaagacg gcgggcaaag atgccgacaa cgacaagccg
     4801 acttatgtga aactgatggg cttggaagcg gcgcgctcat acgcacacaa actggttgcc
     4861 gaagcagtcg cgctgctcga acccttcggc gacaaagccc tgcgcctgcg gcagttggca
     4921 gaatttgcag tcgcacgcaa atattaaaac cggcgtatat cgccgactga aacgtgccgg
     4981 cacagcgatg ccggcctgca cttgaatgaa acaaatgccg tctgaaaggg tttcagacgg
     5041 cattcctatg cttgtttgct tgtttattcc ctgttgcgcg tgtgccaaaa ataccgccgc
     5101 acgaaacacg ggaaggcgaa aatgaggtac aggcagacga cggtggcgta aaactcccaa
     5161 ccctgattgt gtaccgctcc cgcacgggat tcgaggatgt aggcaagaga ggcggtcagc
     5221 gcgaaacctg ccgccagctc gatcaggtgg tgtccgaaat gtttgcgctt gagcgcggcc
     5281 acgccgaaca gtctggtcgt gaggaagggg gcgttggcaa agatgagtgc caagaccaaa
     5341 aggatgtaca tggatgcggt catgatgggc tttctgtttg ggcattgtcg gcggcgatgc
     5401 cgtctgaact gcattgcgga acggcatatc ttaacaaaaa cggcagcctc tgacacggct
     5461 gccgtttcgg cggctcacag cgtgttctcc aacgccttgg cgcaccagtc gataacggtt
     5521 tgcggcataa tgccccacag caccaacagc agcgcgttga cgcttaagag ggattttgcg
     5581 gcattgttgc cgccggcggc gggacgggcg cggccggatt cgtcaaaata catgactttg
     5641 accacgcgca ggtagtagaa cgcgccaacc agcgacatca cgacggcaaa caccgacaac
     5701 catacatagc cttgtttcaa aagcgccata atcacgccga atttggcgta aaaacccatc
     5761 agcggcggaa tgcccgccat agagaacata accagcagca tcaaaaaggc aagccatacg
     5821 cggtgttggt tcaaccctgc caaatcgctg atgttttcgc actcgttgtc cccgtccgac
     5881 aacaccatca acacgccgaa ccctgccgcc gccatcagcg cgtaggtaat ggcgtaatag
     5941 aggcccgccg caaagccgac cgcgcccgcc ataaacgcca acaggatgaa acccgtatgc
     6001 gatacggtgg aataggcgag catacgtttg atattggtct gcatgatggc ggcaaggttg
     6061 ccgaccagca gcgaggcggc ggcaagcagg gcaaacatca gagaccagtc atgatgcacg
     6121 gttcccagcc cggtaacgag gatgcggaaa gcgaaaacga cggcggcgat tttcggggca
     6181 gtgccgacca aggcggtaac agaagtgggc gcgccgtgat acacgtcggg catccacata
     6241 tggaacggta ccgcgccgag tttgaacgca acggcgacga cgataaacac cagacccagt
     6301 ttcaacagcc attcgttagc ttcttcattg aaggaagagg cgagcacgcc ggcaaattcc
     6361 agcgaaccgg ttgcgccgta aaccatagaa ataccgtaga gcagcaggcc ggatgccagc
     6421 gcgcccagaa caaaatattt caaggcggct tcggcggcaa aaccggaatc gcggcgcagg
     6481 gcaatcaggg cgtaaagggc gagcgacaag agttccaaac cgatataggc agttaaaaaa
     6541 tgccccgcgc tcaccatcac gctcatgccc aagagggcaa acagcgacag ggtgtaaaac
     6601 tcgcctttga aaataccgcg cacttggttg tagggcttgg catagacaaa caggacaaac
     6661 gtcaaggcat ataaaaccat ttttgccaaa cgcgacatac cgtccgcaat atacatcccg
     6721 ttgaacgaag acgtgctgcc ctgttcccac acggcaagct gcaccgccgc cgtaaccgcc
     6781 accgttgcca acgcgccgta atgcgtccaa cggcatttgt catcactggc ccacaagtcc
     6841 gccggcaaca ataacaccag tagcgacagc agcgcgactt cgggcagtgc gggcattaaa
     6901 ttcaaatctg accaattcat ttacacacct caaatcttgc tttgcgccac atgggcaatc
     6961 aaatcgtttg ccgcctgatg caccacttcg ataaatgcgt tcggatacag gcccatcccc
     7021 aaaacagcaa ccgccaaaac cgccagaatc gcaaactcgc ggcaattgat gtctttcatt
     7081 tcggcaacgt gcggattgtg aatcgcgccg aaaatcacgc gcttgtacat ccacagggta
     7141 taagacgcgc cgtaaatcag cgtcatagcc gccagcgcgc ccacccagaa attcacttta
     7201 accgcgccca taaccaccat aaactcgccc acgaagccgg aagtcgcagg caggccggcg
     7261 ttcgccatac cgaacaacat cataaacgcc gcaaacttgg gcatcacatt gaccacgccg
     7321 ccgtaatcgg caatattgcg cgtgtgcagg cggtcgtaca tcacaccgat gcacataaac
     7381 atcgccgccg acacgaagcc gtgcgaaatc atctgaatga ccgcgccttt caacgcccag
     7441 tcgtccaact gcccgtcaac aaacaaaaac atcccaagcg ttacaaaacc catatggctg
     7501 atggacgaat acgccaccag tttcttcata tcggtttgca ccagggccac cataccgata
     7561 taaattacgg caatcaggct caatacgatg atcacggggg caaaatagcg agccgcgtcc
     7621 ggcataatcg gcaggataaa gcgcaagaaa ccatacgcac ccagtttcag cgtaattgcc
     7681 gccaacacca tcgaaccgcc ggtcggcgct tcaacgtggg catccggcag ccaagtgtgt
     7741 acggggaaca tcggcacttt tacggcaaat gacaggaaga acgctacaaa caaaagctgt
     7801 tgtacgccca acggaatctg tttgatgttt tggaaatcga caatagagaa gctgcctgtc
     7861 tgatagtaca ggtaaaccat ggcaaccaac atcaagagcg aacccgtcag cgtatagagg
     7921 aacagcttga ccgacgcata gacgcggcgc gggccgcccc atacaccgat aatcaggtac
     7981 agcggaatca gcataccctc gaagaacaca taaaacagaa tcgcatcctg cgcggcaaac
     8041 gcgccgttaa tcaaacccga catcatcagg aatgccgcca tatactgcgc cggacgtttc
     8101 tgaatgactt cccaaccggc caataccacc agcagcgtaa taaacgcatt caagatgata
     8161 aagagcactg aaataccgtc cacgcccaat gcgtagttga ttttcagaag cggaatccac
     8221 tcgtggaact cggtaaattg atagccgccg ctcaaacggt cgaaaccggt aaacaggggc
     8281 agcgttacca agaaaccggc aagcgctccc atgaaggcaa gcacacgcgc cagcggcgca
     8341 cggctgtctt taccggtcgc caaaaccagc acgcctgcgg cgatgggtat ccatattgcc
     8401 aagctgagta ggtagttgga aaacatagtg gttaacctgt ggttaaaata aaaaatatag
     8461 ttgtatgggt tggtctttac agacagcgtt ttaggaagtc gtctgaaaga aggaatgaat
     8521 actctccccc cttctttact tattcccaat caatatctgt acgattgcga tattctgcct
     8581 gtactaaaac atctaattca gccaaatctt ttatttctga ctgcatagct agtaaagctc
     8641 ttaacttcat acttaaatca atatatttgg agccttcgct tgctcctttt gcagacttaa
     8701 atttattttc ttctaaaaat tttttaatgg gagcatttaa aacaggatat ctatctggat
     8761 attcttgaca aagcatttca gaaaaaactg aaccacgagt ggaaacccca cattctttta
     8821 gccaatcaat ttgcttggca accacattat ctctctcagt aagtggagca tcaaaaactg
     8881 attggatggc aatgcataat tttctaaaat ctgaagactt acccgtcctt ttccacgttt
     8941 tatcttgaaa acgattgcct accccttctc ctttattttc gcttttccaa gaccataatt
     9001 tattaaattc cccataaaaa tcattattgt ctatttttcc tgctgcacat tgcttaataa
     9061 gattttttaa ttgattacac acagtttgat ggtttctcat ctgctttctc cgtgccgcaa
     9121 tcagttcttg attataattt ggcatctcca taaataactt atccatcact gattgtctaa
     9181 cagttgattt cttatagtta atttcagctt cctgatactc ctcaagccaa tcttccgata
     9241 ccgggataga tttcatcgca atttcatcag cccaagattt gacttttata aaatcctgct
     9301 cggaaatttt agtcaaaata tttgcttcat aattgctatt aaacgcagca tgagtcaaat
     9361 ttgaagaacc gattaaagca taatattcat tcttatcatt cttccaaaat acagctttag
     9421 gatgaaaacc ctgaatgcct tgagctacca tcagtgtgaa tttcaaatgg gacggaagcc
     9481 atttcaacac tttccgaata gcctcttttt tagacaatcc gaaatcttgt ccaaaaataa
     9541 atttaaactc ttgcaatttg ctattgagct tattttttac atcccaatga gtgagatacg
     9601 cagagaaaat atacagctct actgcctctg aaacagcttt ctcatatatt gaaatcagtt
     9661 ccttctcatt tggtgtatct gaatgtaaaa ataactgtgt catccctttt gactcctcta
     9721 tatttgttga gattgcctaa aaaatttagg aaacagataa tttgcatgtt ggctatttca
     9781 cgcctgttgg gtaccatttt gttccaccct ttgacttaat gcccacttct caaacttttc
     9841 agacgacctc tacaaaacta tgaacaggtc gtctgaaact ccgcctttat ctgaacaagc
     9901 cccagaaggt catgccgagc aataccaata cgccgaacac catagcggcg gcgtaggtgt
     9961 agataaagcc ggtttggact ttacgcactt gcgcggcaat tgcgccgacc agtttggcgg
    10021 agccgttgac gatgccgttg tcgataatgg cggtatcgcc gactttccag aagaagtttc
    10081 ccaatgcgcg cgtgcctttg gcgaagacgt tgaaatacaa ggcgtcgagg tagtatttgt
    10141 tttcaaacaa aacgtaaacc ggacggaacg cctgcgcgat tttagcgggc aggtgcggca
    10201 gtttgacgta caaaagccat gcgctcaata cgcctgcggt agcgagatag aggacgggcg
    10261 aatgcagact gtgcgacacc atcgccaatg cgccgtggaa ctcttccttc atgatgtgca
    10321 tggtcggatg cgcgtcggcg ttgacgaaaa tcacgtcttt gaagaaatcg ccgtagagca
    10381 tgggttcgat ggcgatgtag ccgatgatga cggacggaat cgcaagcaga atcagcggca
    10441 gggtaacgac cagcgggctt tcgtgcggat tgtcgttttt gcccaagccg tgatgttctt
    10501 caccatggcc gtctgaatgg tgttcgggca gactgcgcca tttttcttcg ccgtggaaca
    10561 ccataaagta ttggcggaac gcgtaaaacg cggtaacgaa cacgctggcg aggacggcaa
    10621 aataggcaaa gccgctgccc ggcagggtgc tgtatttcac tgcttcgata atcgaatctt
    10681 tggagtagaa gccggagaag aacggcgtac cgatcagaga caaattgccg atcagcatag
    10741 tcagccaagt aatcggcatg tattttttca ggttgcccat atggcgcata tcttgatcgt
    10801 ggtgcatacc gataatcgca ctgcccgccg ccaagaacag caaggcttta aagaaggcgt
    10861 gggtcatgac gtggaacatc gccacagaat aggcggacgc gcccagagcc acggtcatgt
    10921 agcccagttg cgacagggta gaatacgcaa ccacacgttt gatgtcgttt tgaatcacac
    10981 ccaagaagcc cataaacagg gcggtaatcg cgccgattac cataatgacc gacagcgcgg
    11041 tgctgctcat ctcataaatc ggcgacatac gcgacaccat aaacaaaccg gcggtaacca
    11101 tcgtcgcggc gtgaatcaat gcagaaatcg gagtcgggcc ttccatcgaa tcgggcagcc
    11161 agacgtgcag cgggaattgt gccgatttac ccatcgcacc gacaaacagg agcaaacagg
    11221 ttacggtaat caaagaccat tccacgccgg ggaaaagctg gatagtggca ttttgtacgt
    11281 tgggcagata ggcgaatacg tcctgataac gcaggctgcc gccgaaatag gcaagcacca
    11341 agccgatacc gagcaaaaag ccgaagtcgc cgacacggtt gatcaaaaag gctttcaggt
    11401 tggcaaatgt cgcgcccggg cgtttgaaat agaaaccgat caagagatac gacaccaagc
    11461 ccaccgcttc ccaaccgaag aagagctgga tgaagttgtt gctcataatc agcatcaaca
    11521 tgctgaatgt aaacaaagaa atatagctga agaagcgttg gtagccgact ttttcatcgt
    11581 gcatataacc gatggtatag atatgcacca tcaacgacac gcccgttacc acgaccatca
    11641 tcatcgccgt catcgtatcg accaagaagc cgacggagaa atccaaaccg cccattgtca
    11701 gccaggtata gacgttttca tcaaacttgg cacggctgcc attgaggaat ccccacagca
    11761 cataagccga cagcacggca gatacagcga caccgagtat cgtaaccgta tgcgcgccgg
    11821 cacgcccgat tttgttgccg aacaaacccg caatcaacga gcctgccaac ggaacaaggg
    11881 caattatcaa atataaagtc atatcgttca taattaccct ttcaactcgt ccaaatcggc
    11941 aacattaatc gtttgtcggt tgcggtacac cagcaccata atcgccaaac cgatggcgga
    12001 ttcggcagcg gcaacggtca atacgaagaa tacgaaaatt tgtccggcag tatcgcccaa
    12061 atgttgcgag aaggcgataa agttgaagtt caccgccaaa agcatcagct cgatggacat
    12121 cagcaatacc agcacgtttt tgcggttcat aaagatgccc atcgcgctga taccgaacag
    12181 gagagcgccc aataccagat aatgcgtcaa agtaatcatg ctttgccctc cccttccgtc
    12241 ttgaggccgt ctgaaacttc gctttcttcg gcagattcga cttgcggttt gaccgcttcc
    12301 attttcacca gacgcatacg gccttgatcg gcgcgtactt taacttggtc ggctggatcc
    12361 atacgtttcg gattggtggt tttgcggtga accaatgcaa tcgcggccac catgcccaac
    12421 agcagcaata ccgccgccaa ttcaaacggc aacaggtagt cggtataaat acggctgccc
    12481 aaatcgcgga tattgttgta atcggccgga atgtctttca tcagaccaaa tgcggccagg
    12541 tcggttttcg ggttgaccag aatcaggatc agggcaaccg ccaacaacgt accgaccaca
    12601 ccggcaacag gcgcgtgccg ccagaaaccg gcgcgcattt cctcgatgtc gatattcagc
    12661 atcatcacga cgaacaggaa caacaccatt acggcgccga cgtaaaccac caccagcgta
    12721 acgcccaaaa actccgcctg catcagcatc caaatcatcg cgctcacgca gaaggtcagc
    12781 accagatgca aagcggcatg gacagggttt ttggcggtaa cggtacgaac cgcaccgtac
    12841 aaaacgatgg cggcaaggat atagaacaga atcacggaaa aagtcattcg tctgctcctt
    12901 agcggtacgg cgcgtcagcg gctttgcgtt tggcgatttc ggcttcgtat ttgtcgccga
    12961 ttgccaagag gatgggcttg gtcatatgca agtcgccttt tttctcgccg tggtattcaa
    13021 aaatatgggt ttccacaatc gcatcggtcg ggcaggcctc ttcgcagaaa ccgcagaaga
    13081 tgcacttggt caggtcgatg tcgtaacgct tggtgcggcg ggtgccgtct tcgcgttctt
    13141 ccgattcgat gttgatcgcc attgccggac acaccgcctc gcacaattta cacgcgatgc
    13201 agcgttcctc tccgttcgga taacagcgtt gcgcgtgcag accgcggaaa cgcacggatt
    13261 gcggcgtttt ctcttcggga aaataaattg tgtctttgcg ggcaaaaaag tttttgagcg
    13321 ttacgcccat gcctttgacc agttcgccaa gcagaaaggt ttttactaag ttagccatat
    13381 tatgttccct caaaacaggg atttcgttag gtattcaaaa tcgctttgtt cagacggcct
    13441 caagatgccg tctgaaactt atttccacaa attcagcggt gaaatcatcc acacgcccaa
    13501 aaccacgatg taggcgaagc cgatcggaat cagcactttc cagcccaaac gcatgatttg
    13561 gtcgtagcgg tagcgtggga aggttgcacg aatccacaga taccagtaca gcaccgccgc
    13621 cattttcacg aacatccaga atgcggaagg cgtaccgaca atgccccagc tttgcgggaa
    13681 cggagacaac cagccgccga ggaacatcag cgatgtcagc gcggcaatca gaatcatgaa
    13741 aatgtattcg gcaaggaaga acagcgcgaa tgcaaagccg gagtattcga cgtggtgtcc
    13801 ggcaacgatt tcagactcgc cctctgccac gtcaaacggt gcgcggttgg tttcggcaac
    13861 ggcggaaatc agatagacga tgaagattgg gaacagcggc agccagttcc acgagaacac
    13921 agaaccgccc gcgatgcctt ttgcctgcgc ggcaacgatg tcggagaagt tcatgctgcc
    13981 cgataccatc acaacgcaca ccagcgcggc actcatggca atctcgtagg aaatgctttg
    14041 cgcggaagca cgcattgcac ccaagaacga atatttggag ttggaagccc agcccgcgat
    14101 aatcacgccg taaaccgaca gcgaggtaat catcaggatg tacaaaaggc cgatattgat
    14161 attggtcagc acccattctt cgttaaacgg aatcaccgcc cacgccgcga aagacggggc
    14221 gagcgacata atcgggccga tgtagaataa ggctttgttt gacagcttcg gacgggttac
    14281 ctctttaaac aagagtttga acacgtcggc aaacggctga atcagacccc gcgggccggt
    14341 tacgttcggg ccgacgcgaa gctgcataaa gccgatgact ttacgttcga aatacgtcag
    14401 gtaggcaacg gtcagaatca gcggaatcag gataatcacg attttgacga tgacggatac
    14461 caccaagcct acggtgatgc ccaaatcgcc cagaccgagc gttgcggcaa agaggttttg
    14521 gaaccattcc tgcataatca agctcccgcc agttcaatag tgcccatcaa cgcacccagc
    14581 gcggcatttt cggtatgcag cggcagatgc accacgtttt caggcaaacc ggcatcggct
    14641 ttgacggcaa ccgatacgct tgcgccgttt tgtttggcga cagcggtttg tccgtcttgc
    14701 aggcccaagc gtgccaatgt gttcggattt acacgcgcgg caggcacggc ggcatggctg
    14761 gtttcttgca acggtgcgga acggcgcacg atagaatcag tatgataaat accgacgccg
    14821 ccgacacgga cgaggcggtc tgaggccgtc tgaacgccct cccctgccca tgtgctgcgg
    14881 ttgtccagtt tggacggcag gctttccgca tccagcgcgt ctttcagaat cgcggcggta
    14941 tcgtggtatt caaaaccttt caggtcaaac aggttgccca atacgcgcaa cactttccac
    15001 aacggacgtg aatcgccgaa gccttgtacc acgccgtgga aagattgcag acggccttcc
    15061 atattgatga agctgcctga tgtttcggta aacggcgcaa tcggcagcaa tacgtcgcac
    15121 acgtccagca gcgtttcgct gacaaacggc gtaaacgcca tcacgctttt cgcctgtttc
    15181 aacgcggcta cggcttttgc accgtcaacc gtatcgattt caggctcgac gttgagcagc
    15241 aagactgcct gtttcggcgt gtttgccatt tcggcaacgc tctcgcccga gttcacaccc
    15301 aatacgtccg caccgacgct gttggcggct tgcggcaaaa tgcccagcac tgcgccggtc
    15361 gcgtccgcca actcttgcgc ggcggcataa atggcggcgt aatcaggatg gttttgcact
    15421 tccgcaccca aaatcaccgc tgctttttca gcgtttttca ggctggcggt aacggcatgt
    15481 tccgcatcgg cagacaggtt tttcagacgg cctgcccact cgtcgggatg tgcggcttct
    15541 tgagacagaa gcggcataaa caattcttct ttactgccgg ccaatacgct caaggccata
    15601 cggtctttgg cggcgcggcg caggcgggcg gtcaggagcg gctgttcttt gcgcaagttc
    15661 gcaccgacta ccaatacggc atcgttgtca gccaaagatt caatactttg tcccaaccat
    15721 tgcgcacctt taaggccgtc tgaaagacgt ttgtcttgtt ggcgcaaacg ggttgcaaag
    15781 tttttaacac ccaagccgtc ggcaaatttt ttggccagat acagctcttc aaccgtattc
    15841 atcggattag cccaaacgcc gacttggttt tggttgccgt ctttggcgat acattcaatc
    15901 gcgctgcgga catattccaa cgcggttttc caatccacgt ccatccactc gccaccctgt
    15961 ttgattttcg gatttttcag acggctttcg tgatacaggc cttcgtaggc aaaacggtcg
    16021 cggtcggaca gccagcattc gttgatggct tcgttttcca acggcaacac gcggcggacg
    16081 gtatggtctt tggtttgtac aatcaggttg ctgcccaaag cgtcgtgtgc ggaaacggat
    16141 ttgcggcggt tcaattccca agtacgcgcg ttgaagcgga acggtttgct ggtcaatgcg
    16201 ccgacgggac acaaatcgat gacgttgccc gacagctcgg tttccaccgc tttgccgata
    16261 aagggcatga tttcggagtg ttcaccacga ttcaccatcg caatttcctg caaaccggcg
    16321 atttcttcgg tgaaacgtac gcagcgggta cagtggatac agcggctcat ttcctcggcg
    16381 gaaaccaaag gacccatgtc cttgccgacg acggaacgtt tttcttcggt ataacggctg
    16441 gtggttttgc cgtagcccac cgccaaatcc tgcaactggc attcgccgcc ctggtcgcag
    16501 gtcggacaat caagcggatg gttgatgagc aggaactcca tcacgccttc ttgcgcctct
    16561 cgggcttttg ccgaatgcgt acgcacaatc atgccgtccg ttaccggcgt ggcacaggca
    16621 ggcagcggtt tgggggcttt ttccacatcc accagacaca tacggcagtt ggcggcaata
    16681 gagagctttt tgtggtaaca gaaatgcgga atataagtac cgagcttgtg cgcggcttca
    16741 attaccgtcg cgccctgctc cacagatacc tgtttgccgt cgatttcgat ttgtaacatg
    16801 gttcgttcct agttacgggt atttgataaa taatttttta tggagatcgt ctgaaaaatg
    16861 ggcttcagac ggccttttga gtttcattga caatcaatac tgtttaaaac gtttactgcc
    16921 accttgccgt cattcccgca caggcgggaa tccatttttt gaatttcggc aactgcttt
//
LOCUS       DBJGGI010000045        16829 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.045, whole genome shotgun sequence.
ACCESSION   DBJGGI010000045 DBJGGI010000000
VERSION     DBJGGI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16829
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.045"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(319..1248)
                     /locus_tag="ACSIGJ_001442"
     CDS             complement(319..1248)
                     /locus_tag="ACSIGJ_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HHQ1330284.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQSSIS
                     FHPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            1377..2126
                     /locus_tag="ACSIGJ_001443"
     CDS             1377..2126
                     /locus_tag="ACSIGJ_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HHQ1330285.1"
                     /translation="MVLRRDFLAWCDETLQTASFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     VHPILGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVAI
                     GNPEREIRRVAWCTGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            2249..2830
                     /gene="petA"
                     /locus_tag="ACSIGJ_001444"
     CDS             2249..2830
                     /gene="petA"
                     /locus_tag="ACSIGJ_001444"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HHQ1330286.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            2849..4198
                     /locus_tag="ACSIGJ_001445"
     CDS             2849..4198
                     /locus_tag="ACSIGJ_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HHQ1330287.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            4213..5001
                     /locus_tag="ACSIGJ_001446"
     CDS             4213..5001
                     /locus_tag="ACSIGJ_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HHQ1330288.1"
                     /translation="MKQAMKNWFAALLSAVPMSAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWGQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRTGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            5333..7123
                     /gene="rmuC"
                     /locus_tag="ACSIGJ_001447"
     CDS             5333..7123
                     /gene="rmuC"
                     /locus_tag="ACSIGJ_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HHQ1330289.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFAGLNAQLAEKAA
                     RCDFIEQAHAEIASELAALDGKYEHLQDENYALGNRFSAAEKQIAHLQEKEAESARLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAYLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVRQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADEAARELAAYIASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERANEQTAFALGKPSEQEAADEAE"
     gene            complement(7190..7822)
                     /locus_tag="ACSIGJ_001448"
     CDS             complement(7190..7822)
                     /locus_tag="ACSIGJ_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HHQ1330290.1"
                     /translation="MTLRYEILPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLETHKVPVLGPHREDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHVVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFAFPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            7915..8739
                     /locus_tag="ACSIGJ_001449"
     CDS             7915..8739
                     /locus_tag="ACSIGJ_001449"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HHQ1330291.1"
                     /translation="MIKKIIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRRHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNLPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            8808..9371
                     /locus_tag="ACSIGJ_001450"
     CDS             8808..9371
                     /locus_tag="ACSIGJ_001450"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HHQ1330292.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVAYEITRDLGD
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            9454..9891
                     /locus_tag="ACSIGJ_001451"
     CDS             9454..9891
                     /locus_tag="ACSIGJ_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HHQ1330293.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEGQD
                     DIINNAISALQEFPENHGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMIKAV
                     QYSPAAEDFAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            10112..10381
                     /locus_tag="ACSIGJ_001452"
     CDS             10112..10381
                     /locus_tag="ACSIGJ_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HHQ1330294.1"
                     /translation="MLKQSIEIINKLGLHARASSKFTQTASQFKSEVWVTKNGSRVNG
                     KSIMGLMMLAAAKGTVIELETDGLDEAAAMKALTDLINDYFGEGE"
     gene            10381..12156
                     /gene="ptsP"
                     /locus_tag="ACSIGJ_001453"
     CDS             10381..12156
                     /gene="ptsP"
                     /locus_tag="ACSIGJ_001453"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HHQ1330295.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAQADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIISPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGEVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPSVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNAGQLEGDIAKVIRCEDE
                     EKSEKLIKQINSVSVAEEAEVKGRK"
     gene            complement(12283..13224)
                     /locus_tag="ACSIGJ_001454"
     CDS             complement(12283..13224)
                     /locus_tag="ACSIGJ_001454"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHQ1330296.1"
                     /translation="MLELNGLCKCFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIRLNGENITCMPPEKRRISLMFQDYALFPHMSALENTAFGLKMQK
                     MPKAEAERLALSALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LIQTPAGVQVARLMGLPNTDDDRHIPQNAVCLDNHGTECRLLSLVRLPDSLRLSAVHP
                     EHGELTLNLTVGQHTDGISGNGTVRIRVDEGRIVRFR"
     gene            complement(13233..14108)
                     /locus_tag="ACSIGJ_001455"
     CDS             complement(13233..14108)
                     /locus_tag="ACSIGJ_001455"
                     /inference="COORDINATES: protein motif:HMM:NF012514.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HHQ1330297.1"
                     /translation="MPTVRFTESVSKQDLDALFERAKASYGAESCWKTLYLNRLPLGN
                     LSPEWAERIKKDWEAGCSESSDGIFLNADGWPDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLVESNGRWHFWIGRRSPHKAVDPGK
                     LDNIAGGGVSGGEMPSEAVCRESSEEAGLDKTLFPLIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVAGFEKMDIGGLLDAMLSKNMMHDAQLVTLDAFYRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(14291..14367)
                     /locus_tag="ACSIGJ_001456"
     tRNA            complement(14291..14367)
                     /locus_tag="ACSIGJ_001456"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14331..14333),aa:Met,seq:cat)
     regulatory      14516..14615
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            14706..16607
                     /gene="thiC"
                     /locus_tag="ACSIGJ_001457"
     CDS             14706..16607
                     /gene="thiC"
                     /locus_tag="ACSIGJ_001457"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HHQ1330298.1"
                     /translation="MTTPKKTAKISGNEARELSDLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGAYGDPAAHIDLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGIECAHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPE
                     MEFAAIRERMKLDELFRRPEYAKLLKQHTGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQTLEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         4053 a         4399 c         4657 g         3720 t
ORIGIN      
        1 agaaagaata aaacatcgat aaatctcttt tcctccctct tgtagaataa tatatttttg
       61 ataggtgttt attaaatagt cttctaactc tcttatggaa ttataaggat atttcgttaa
      121 tctcattatt ttgttccatg tctaaaacgt cctgttgatc catatatttc acccgttatg
      181 agtctgttcc catctcatat agtttcaagt ttaacaaccc cttgaggcta tgttaaaaga
      241 gtttttctaa taactattcc gttttcttaa atataaaaaa atagaaaaat agcccgggta
      301 tagtttgaac aagggaaatc agaccggttc caactcactt aatccgggca agtccgcgaa
      361 gccgcgttca cgtatgattt ggcaaaagtt gttcagataa cttttatctg tgtcttcagt
      421 gcggatggcg gcatacagtt tgctttgcag tccgtcggca gtaatttggc ggtggacgac
      481 atagcctttt tcaaggtagg gcatgactgt ccaataggga agggcggcaa tgccacgtct
      541 gctggcaacc agttggataa tggcgatggt cagctcgctg tgccggcgcg gcgggttgat
      601 gtttttcgga atcaggattt ttttgggtaa atccagcatt tcatcgggaa cgggataggt
      661 aatcagggtt tccccgataa agtcttccgc cgtccaaacg tttttggcgg caagcggatg
      721 gtccggggcg caaatgccga ccatttcgta ggcaaacagt gggtggaagc tgatgctgct
      781 ttgtttttcg gcttcggaga caatggcgag gtcggcgcgg tgttgcagca gcagcccgac
      841 gggatccgct tggaatcctg atacgatatc cagttcgact tggggccaca tcgggcggaa
      901 ttcgcccatg gcgggcatca gccagtcgaa acaggtatgg cattcgacgg caatccgcag
      961 ctctcccgcc tccccttccg tgattcgcgc caaatctcgt tctgcaacag caacttgagg
     1021 tatcagttcg tgggcgaggc gcagcagcct ttcgcccacc ggggtaaagc gcaggggcgt
     1081 ggatttgcgt tcgaacagcg gcgtgccgta gtggttttcg agcatacgga tctggtggga
     1141 aagggcggat tgggtaagga aaacccgttt ggcggcaagg gagacgctgc cggtttcttc
     1201 aagtgccagc agggttttga ggtggcgcaa ttcgataatg gaatccatgg ggcttcagac
     1261 ggcatattga accggcgcat attaaaataa ttcatatgcg ggtgcaaatc ggaaaaatgg
     1321 gcgtttcggg catttgcggt tagaatgccc gcttgtttgg aaatgacagg aaaaatatgg
     1381 ttttgcgcag agatttttta gcctggtgcg acgaaacgtt gcagacggca tcgtttaaag
     1441 attacgcccc caatggtttg caggttgaag ggagggaata tatcgggaaa atcgttacgt
     1501 cggtaacggc aagcagggca gcgattgatt ttgctgtgga gcagaaggca gatttgcttt
     1561 tggtacatca cggtatgttc tggaaaaacg agctgccgac cgttaccggt tggaaaaaag
     1621 aacggattgc cgcactgtta cggcacgaca tcaatatggc aggctaccat ctgcccctgg
     1681 atgtgcatcc catactgggc aacaatgccc aactcgccga cagattgggt tttgcgacag
     1741 aaaaacggtt cggcgaacaa aacctgctca actcgggcag cctgaaacaa gccaagacac
     1801 tcggcgcatt ggcggcgcat attgaaacag ttttgcaacg taagcctgtc gccatcggca
     1861 atcccgaacg cgaaatccga cgggttgcat ggtgtaccgg cggtgcacag ggcttttttc
     1921 agacggcaat agatgaaggc gtcgatctgt atctgacggg tgaaatttcc gaagcccaat
     1981 accacctcgc caatgaaacg ggtacggctt tcatttcggc agggcatcac gcgacggaac
     2041 gttacggcgt acgcgcgctg gcagaatcgg cggcagaggt tttcgggttg gaagtgtgcc
     2101 attttgacga aaacaacccg gcttgagtct tgagaaatat cataaaactt taccttattt
     2161 taaataatgc tttgagtatc actgcaaact gggtaaaatt gcaatgttta atggctcggc
     2221 attcccaaaa tattaggacg actgaataat ggataatcaa gaaatcaaca acggccgccg
     2281 ccgtttcctg acacttgcga cctgcggcgc gggcggagtg gcagcattgg gtgtggcaac
     2341 gccgtttgtg gccagttttt tcccttcgga aaaagccaag gccgccggtg ctgccgtcga
     2401 ggtggatgtc agtaaaatcg aagcgggtca gctgctgacc gccgagtggc aaggcaaacc
     2461 gatttgggtg ctcaaccgta cagatcagca gcttaaagac ctgaaaggcc tgaacggcga
     2521 acttaccgat cccaattccg atgcggaaca gcagccggag tatgctaaaa acgagacccg
     2581 ttcgattaag ccgaacatcc ttgtcgccat cggtatctgc acccatttgg gctgctcgcc
     2641 caccttccgt cccgacattg cccccgccga tttgggcgca gactggaagg gcggcttctt
     2701 ctgcccgtgc cacggttcga aattcgactt ggccggccgc gtatataaag gtgttcctgc
     2761 cccgaccaac ctggttgtcc cgccatataa atacttgagc gacacaacta tcttggtggg
     2821 cgaagactaa gaataaggaa cgataattat ggcaaaccaa accaatagca aagcaaaagc
     2881 attgttaggc tggatggacg ctcgcttccc cctgtctaaa atgtggaatg agcatttggc
     2941 gcaatactat gcgccgaaaa actttaattt ttggtactat ttcggctctt tagccctgct
     3001 cgtcctcgtg attcaaatcg tcagcggtat tttcctgacg atgaactaca aaccggacgg
     3061 caacctgaat gcctaccatc tgcctgctgc ctttaccgca gtagagtaca tcatgcgcga
     3121 cgtgtccggc ggttggatta tccgctacat gcactccacc ggcgcatctt tcttcttcat
     3181 tgttgtttat ctgcacatgt tccgtggttt gatttacggt tcatataaaa aaccgcgcga
     3241 attggtgtgg attttcggtt ctttgatctt cttggcattg atggcggaag cctttatggg
     3301 ctacctgctg ccttggggtc agatgtcctt ttggggtgcg caggtgatta tcaacctgtt
     3361 ctccgccatt cccgtcatcg gtcctgattt gtccacttgg attcgcggcg acttcaacgt
     3421 ttccgacgtt accctgaacc gcttcttcgc cctgcacgtt atcgccgtac cgctggtatt
     3481 gctcggttta gtcgtggcac acatcatcgc actgcacgaa gtgggctcta acaaccccga
     3541 cggtgtggaa atcaagaaaa acaaagatga aaacggcatt ccgcgcgacg gcatcccgtt
     3601 ccacccttac tacactgtta aagacatcct gggcgtcgtt gtattcctga ttgtcttctg
     3661 tgccgtattg ttctttgccc ctgaaggcgg cggctacttc ctcgaagcgc cgaacttcga
     3721 tgcggcaaat gcgctgaaaa cacccccgca cattgcgccg gtatggtatt tcacaccgtt
     3781 ctacgccatt ttgcgtgcga ttccttcctt cttgggaacg caggtttggg gtgtaatcgg
     3841 tatgggcgcg gcggtcgtgt tgattgccct gctgccttgg ctggacaggg gcgaggtgaa
     3901 atccgtccgt taccgcggtc cgattttcaa aaccgcattg gtcttgttca tcatttcctt
     3961 tatcggtttg ggtattttgg gcgcgatggt ggcaaccgat acgcgtactt tggtcgcgcg
     4021 catcctgtcc ttcgtctatt tcgcattctt cctgggtatg ccgttctata ccaagctgga
     4081 taaaaacaaa ccggttcccg aacgcgtaac catgagcacc gccaaacaaa aaatcatgtt
     4141 ctttgtttac gtcggcatta ctgccgtggg tgcttacctg tttgcaacca acatctgatg
     4201 agggcagtcg aaatgaaaca agctatgaag aattggtttg ctgccttgct gtcggcagta
     4261 cccatgagtg cggcatttgc ttcgggcggt catgcacact atgagaaagt cgatattgat
     4321 ttgcgcgacc aagtaagctt gcagcgcggc gcacaaatct ttaccaacta ctgtttgtcc
     4381 tgccactcgg caagcggtat gcgtttcaac cgtttgaaag acatcggtct gactgacgaa
     4441 gaaatcaaga aaaacctgat gtttaccacc gataatgtcg gcgatgtcat gcattcggcg
     4501 atgaacccga aagatgcggc aaaatggttt ggtgctgctc cgcccgattt gacattgatt
     4561 gcgcgttcca aaggtgcaga ctacctttac gcttatatgc gcggcttcta taaagatccg
     4621 acccgtccga gcggctggaa caatactgta ttcgataaag tcggtatgcc ccacccgttg
     4681 tgggggcaac aaggcgttca agccgttgag ttggatgcca aaggtcagcc ggttatggta
     4741 aaagacgaac acggcgagat gaagcctaag ctgtattggg aatctaccgg tttgcacagc
     4801 cgccgtctgc ctaacggcaa agtgatccag aaagagtacg acgcatatgt acgcgatttg
     4861 gtcaattacc ttgtgtacat gggcgaacct gcacaactgc aacgcaaacg tacaggttat
     4921 gtcgtgatga ttttcctatt tgcggttatg ctgcctttgg cgtatttcct gaaaaaagaa
     4981 tattggaaag acgtacacta agcgtttgga acaaaagggc aaatccttta gggtttgccc
     5041 ttttttcatt ttgcctgccg tttgaaaagc ctgaatccgt atgccgtctg aacatagctg
     5101 caacatttca gacggcatca ttctaaaaat gtcagacatc gggaacttaa tcagggtttg
     5161 tggcagattg atattgaatt aggaaaaggt ctgaaaccgc aagcagcatg gtaaggcgat
     5221 gtcgaacagg tttaaagcca atccgctata ttttttggag ctgcaccgtg caaacaggta
     5281 gaatggcggc ggttttgtcc ggtgtgaaaa tgtttgaata ttaggatgat tgatggagct
     5341 gatgactgtt ttgctgcctt tggcggcgtt ggtgtcgggc gtgttgttta catggttgct
     5401 gatgaagggc aggtttcagg gcgagtttgc cggtttgaac gcgcaattgg cggaaaaggc
     5461 ggcaagatgt gattttattg aacaggcaca cgcagaaatt gcatcagaat tggccgcttt
     5521 ggatgggaaa tacgagcatt tgcaggacga aaattatgct ttgggcaacc gtttttccgc
     5581 agccgaaaag cagattgccc atttgcagga aaaagaggcg gagtcggcgc ggctgaagca
     5641 gtcgtatatc gagttgcagg aaaaggcaca gggtttggcg gttgaaaacg aacgtttggc
     5701 aacgcagctc ggacaggaac ggaaggcgtt tgccgaccaa tatgccttgg aacgccaaat
     5761 ccgccaaaga atcgaaaccg atttggaaga aagccgccaa actgtccgcg acgtgcaaaa
     5821 cgacctttcc gatgtgggca accgttttgc cgccgccgaa aaacagattg cctatttgca
     5881 ggaaaaagag gcggaagcgg agcggttgag gcagtcgcat accgagttgc aggaaaaggc
     5941 gcagggtttg gcggttgaaa acgaacgttt ggcaacgcaa atcgaacagg aacgccttgc
     6001 ttctgaagaa aagctgtcct tgctgggcga ggcgcgcaaa agcttgagcg atcagtttca
     6061 aaatcttgcc aacacgattt tggaagaaaa aagccgccgt tttaccgagc agaaccgcga
     6121 gcagctccat caggttttga acccgttgaa cgaacgcatc cacggtttcg gcgagttggt
     6181 caggcaaact tatgataaag aatcgcgcga gcggctgact ttggaaaacg aattgaaacg
     6241 gctccaaggg ttgaatgcgc agctgcacag cgaggcaaag gccctgacca acgcgctgac
     6301 cggcacgcag aacaaggttc agggcaattg gggcgagatg attctggaaa cggttttgga
     6361 aaattccggc cttcagaaag ggcgggaata tgtggttcag gcggcatccg tccgaaaaga
     6421 ggaagacggc ggcacgcgcc gcctccagcc cgacgttttg gtcaacctgc ccgacaacaa
     6481 gcagattgtc attgattcca aggtttcgct gacggcttat gtgcgctaca cgcaggcggc
     6541 ggatgcggat gaggcggcac gcgaactggc ggcgtatatc gccagcatac gcgcgcatat
     6601 gaaaggcttg tcgctgaagg attacaccga tttggaaggt gtgaacacat tggatttcgt
     6661 ctttatgttt atccctgtcg aaccggccta cctgctggcg ttgcagaatg acgcgggctt
     6721 gttccaagag tgtttcgaca aacggattat gctggtcggc cccagtacgc tgctggcgac
     6781 tttgcggacg gtggcgaata tttggcgcaa cgagcagcaa aaccaaaacg cgctggcgat
     6841 tgcggacgaa ggcggcaagc tgtacgacaa gtttgtcggc ttcgtacaga cgctcgaaag
     6901 cgtcggcaaa ggcatcgatc aggcgcaaaa cagctttcag acggcattca agcaacttgc
     6961 cgagggacgc gggaatctgg tcggacgcgc cgagaaactg cgtctgttgg gcgtgaaggc
     7021 aggcaaacag cttcaacggg atttggtcga gcgtgccaac gaacagacgg catttgcttt
     7081 gggcaaacct tcggaacagg aagcggctga cgaagccgaa taggaaaggc ggatgcgttt
     7141 cagacggcat cagtgcagat gatgccgtct gaagactttg ggaagtcggt tagaaaaacg
     7201 gattgtgccg tttttcgtgt ccgatggaag tcatacgccc gtgtccggcg acaacttgca
     7261 cggtttcggg gaaggcgaat aatttgttgc ggatattgtt gattaagtcg gcgtggttgc
     7321 cgcgcggaaa atcggtcctg cctatggttt cgtaaaacag cacgtcgccc gcaatcagca
     7381 gctccgcttc ggcacaatag aaaaccacgt gtccgggcgt gtgccccgga atatgcagca
     7441 cttgaaaggc atagcgtccg accgtgagcg tttcgccttc ttcgagccaa cggttcggcg
     7501 caaaggcggg cgagacggga aatccgtatt gcgcggtggt ttgcggcagc gattggagca
     7561 ggaattcatc ttcgcggtgc gggccgagga cggggacttt atgcgtttcc agcatctcga
     7621 ccacgccgcc cgcgtgatcg agatgaccgt gcgtcagcca gattgccgtg agcgtgagtt
     7681 tgcggtttgc caacgcttgc agcaggaacg gcacgtcgcc gccgacatcg gtcaggacgg
     7741 cttcgccgct ttcgtcgtcc caaatcaggg tgcagttttg gcggaagggg gtaacgggga
     7801 gaatttcgta acgtagggtc atcggcgtgt tcctaaaacg gtttttcaga cggcatcggg
     7861 tttgccgttt gttttatggc ggtttgccgc ctgttttgat attgggggaa tcaggtgatt
     7921 aagaagataa tcggcggcat cataccgatt tttacggcgg ttttcatccc tgcatcggca
     7981 ggcgcggcgg atttgatgct ggcgcaggaa tacaaagggc aggacattgc cggctgggcg
     8041 atgagcgaga agctcgacgg cgtgcgcgcc tattgggacg gaaagcacct gataagccgt
     8101 cagggctacg cgtttacgcc ccccaaaggc tttaccgcgc agtttccgcc ttatcctttg
     8161 gacggcgaat tgtatagcgg gcgcggtcag ttcgagcaga tttccgctac cgtgcgttct
     8221 gtttcttcag actggcgcgg catccgcctg cacgttttcg acgtacccaa ggcgcagggc
     8281 aacctctacc aacgtttggc agtcgcaacg cagtggctga aaacgcatcc gaacgcgccg
     8341 attaccatca tcccgcaaat caaagtgcgc gaccggcggc acgcgatgga ctttttaaaa
     8401 caaatcgaag cgcagggcgg cgaaggcgtg atgctgcgcc agcccgaatc ccgttacagc
     8461 ggcggcagga gcagccaatt attgaagctg aaaagccaat acgacgacga atgcacggta
     8521 acgcggcact atgagggcaa agggcgaaac gccggacggc tgggcgcggt cggctgcaaa
     8581 aaccgacacg gcgaattccg catcggcagc ggtttcaagg acaaagaccg cgacaacctg
     8641 cccaaaatcg gcacgctgat tacctaccgt taccgtggct ttacgcggaa aggcacgccg
     8701 aaatttgcca catttgtgcg cgtgcgtacc gaccgctgag caggacagtt cagacggcat
     8761 ccgatacttt ggtttataat tttcctttta ccgaccgatt ccgacatatg accgatttag
     8821 aaaccaaacg ccttgaaaca caggcgatgc ttgaaaacgc cgatcttttg ttcgaccaag
     8881 gccaatgccg tgccgcactg caaaaagtgg cgtacgagat tacgcgcgat ttgggggata
     8941 aatatccgtt gctcctgccc gtgatgggcg gggcggtggt gtttacgggg cagttgctgc
     9001 cgctgttgcg ttttccctta gattttgatt atgttcacgt ttcccgttac ggcgacaagc
     9061 tggagggcgg cgcgttcaac tggaagcgta tgcccgatgc ggaacaaatc cggggcaggc
     9121 acgtcgtcgt gctggacgat attttggacg aagggcatac gatgtccgcc attcaagcca
     9181 aacttttgga aatgggtgcg gcaagctgcc gtgcggcggt gttcgccaac aagctgattg
     9241 acaaacccaa gcctatccga gccgattatg tcggactgga tgtgccgaac cgttatgttt
     9301 tcggttacgg catggatgcg gcgggctgct ggcgtaattt gggcgagatt tacgcattgg
     9361 acggaaaata aggacgcgat gccgtctgaa ggcctttcag acggcatcgc gcccatacgc
     9421 cggcaggatt aatgaggaac aggacgcatc aatatgatag ggcttttaat catcacacac
     9481 gaaaccatag gcgaagccta ccgcaagctg gcgcatcatt tttttccggg cgggctgcct
     9541 gaaaacgtcc gcatactcgg cgtgcagccg acggaaggcc aagacgacat tatcaacaac
     9601 gccatttccg cgctgcaaga gtttcctgaa aaccacggtg tattgattat gaccgacatc
     9661 ttcggcgcga ccccctgcaa tgccgcccgc cggctcgtgc gcgaaaacaa atcggcgatt
     9721 ttgaccgggc tgaacgcgcc gatgatgatt aaggccgtcc aatattcgcc ggcggcggaa
     9781 gactttgccg cctttaccga atgcgtcagg gaggcggcgg taaaaggcat tttcgccatc
     9841 acgtccgcgc ccgaagattt ggtgtgccgg cgcagcggcg atgccgtctg aagaagcggc
     9901 agggcaggaa aacattttaa acggcgtctg ctgccgatat atagcggatt aacaaaaatc
     9961 aggacaaggc ggcgggccgc agacagtaca aatggtacgg aaccggttcg cctggtgctt
    10021 cagcacctta gggaatcgtt ccctttgagc caaggcgggg caacgccgta ctggtttttg
    10081 ttaatccact atacataaca cgggaaccga aatgctcaaa caatccatcg aaatcatcaa
    10141 caaactcgga ctccacgccc gcgcgtccag caagttcacc caaaccgcgt cccaattcaa
    10201 aagcgaagtc tgggttacga aaaacggcag ccgcgtcaac ggcaaaagca ttatggggct
    10261 gatgatgctc gcagccgcca agggtacggt catcgaactg gaaaccgacg gcttggacga
    10321 agccgcagcc atgaaggcat tgaccgactt aatcaacgac tacttcggcg agggcgaata
    10381 atgagtatcg tgctgcacgg cgtggcggcg ggcaaaggca ttgccgtcgg ttgcgcccac
    10441 ctgattgcgc gcggtacgga ggaagtgccg cagtatgatg ttgctcaggc ggacaccgat
    10501 gccgaagccg aacgtttcga tgccgccgtc aaagccacgc gcaaagagtt ggaacagctc
    10561 cgcagcgcga ttcccgaaaa cgccccgacc gagttgggcg cgttcatctc gctgcacctg
    10621 atgctcttga ccgatgttac cttgtcgcgc gaacccgtcg atattttaag ggaacaaaaa
    10681 atcaacgccg agtgggcatt gaagcagcag agcgacaaac tcgccgccca attcgacaat
    10741 atggacgatg cctatttgcg cgaacgcaag caggatatgc tgcaagtcgt ccgccgcatc
    10801 cacaacaacc tgatcgggca gggcaacgag ttggaggttg ccgacaacct gtttgacgaa
    10861 accgttctga ttgcaaacga cctttcaccc gccgacacgg ttttgtttaa agagcagcgc
    10921 attgccgcct tcgttaccga tgccggcggc cccaccgggc atacggcgat tttgggcagg
    10981 agcttggaca tcccgtccgt cgtcgggctg cacaacgcgc gcaaactgat taccgaaggc
    11041 gaaacggtca tcgtggacgg catcaacggc gtgttgatta tttcgccgga tgagtcggtg
    11101 ttgaacgaat accgccgccg cgcccgcgaa taccgcagcc acaaacgcga tttgaacaag
    11161 ctcaaaaaaa ccgccgccgc caccgccgac ggggtctgca tcgagcttgt gggcaatata
    11221 gaatccgccg aagacgtgaa accgctgcac aacctcggcg cagacggcat cgggctgttc
    11281 cgcagcgagt ttctttacct gaaccgcgat acgatgccgt ctgaagacga gcagtacgaa
    11341 gtgtacagcg cgattgtcaa aaaaatgaag ggcaaaagcg taacgatacg gacagtcgat
    11401 ttgggtgtgg acaaaaatcc gcgctggttc gggaaaaaca gcacgcccaa cggcagcctc
    11461 aaccccgcgc tgggcatgac cggcatccgc ctgtgccttg ccgaaccggt catgttccgc
    11521 acgcagatgc gcgccatcct ccgtgcggcg gtacacggtc ccgtccgcat gatgtggccg
    11581 atgattacct ccgtatccga agtgcgccag tgcctcatcc acctcgacac cgcgcaacgc
    11641 cagcttgccg aacgcggcga tgccttcggc gaagtcggca tcggctgcat gattgaaatt
    11701 ccgtctgccg cgctgaccgt cggcagtatt ttgaaactgg tcgatttcat ctccgtcggt
    11761 accaacgatt tgatccaata tattctgtcc gtcgatcgcg gcgacgacag cgtcagccat
    11821 ctctaccaac ccggacatcc ttccgtgctg aaaatgctgc aacacgtcat ccgtaccgcc
    11881 aaccgcatgg acaaagacgt gtccgtatgc ggcgagatgg cgggcgatac cgcgtttacc
    11941 cgcgttttat tgggtatggg gctgcgccgt ttttccatga atccgaacaa catcctgccc
    12001 gtcaaaaaca tcattctgca cagcaatgcc ggacagctcg aaggcgatat tgcgaaagtc
    12061 atccgctgcg aagacgaaga gaagtcggaa aagctgatca aacagatcaa cagcgtgtct
    12121 gtcgcggaag aagccgaagt caagggacgg aaataaatat tgttgacaat gcgagcgggt
    12181 tgcagtcaaa taacgccctt aaaaaataca gctgctttgg tcaagaaatg ccgtctgaaa
    12241 aattttcaga cggcattttg tttggcaggc atttctgccg catcatcgga aacggacgat
    12301 acgcccttca tcgacgcgga tgcggaccgt accgtttccg gaaataccgt ccgtatgttg
    12361 tccgacagtc aggtttaagg tcagctcgcc gtgttcggga tggacggcgg aaagccggag
    12421 cgagtcgggc aggcggacga gggacagcag gcggcattcc gttccatgat tgtccaagca
    12481 cacggcattt tgcggaatat ggcggtcatc gtcggtattg ggcagcccca tcagacgggc
    12541 gacctgcacg ccggcaggcg tttgaatcaa ggtttcgggc gtaccgcatt gaaggatttt
    12601 cccctcgtgc atgacggcga tttcgtccgc cgccgtgcag gcctcttcgg gcgaatgcgt
    12661 taccaaaacg gcagggatgc cgcccttgcg gatgcgttcg gcggtcatac ggcgcagccg
    12721 gtcgcgcaaa tgcgtgtcca aactggaaaa cgattcatcc agcaacagca gggaagggcg
    12781 gacaaccaaa gcgcgcgcca gtgccaaccg ttgcttctcg cctccggaaa gtttttcagg
    12841 cttgcggtgc gcctcgtttt ccagcccgac ttcggcaagt gccgacaagg cgaggcgttc
    12901 ggcttcggct ttcggcattt tttgcatttt caaaccgaat gccgtatttt ccagcgcact
    12961 catatgggga aacagcgcgt aatcttgaaa catcagcgag atacggcgtt tttcgggcgg
    13021 catacaggta atgttttccc cgttcagccg aatttcgccg ccgtccggcc ggacgatgcc
    13081 cgcaatcata ttcagcaggg tggatttgcc gcagcccgac cgccccagta cggcgagtat
    13141 tttgccgcgc ccgacagtca ggcagatgtt gtcggcgacc gttttgccgc cgaagcattt
    13201 gcagagtccg ttcagttcaa gcatggcgca tcctataaac gtatgccgtc cagccactcg
    13261 gacagcggat gggcggcatc aatcagaccg taacggtaaa acgcgtccag cgtaaccagt
    13321 tgcgcgtcgt gcatcatgtt tttcgacaac atggcatcca ataggccgcc aatgtccatc
    13381 ttttcaaaac ccgctacctc gccatcctga ttttcaggca ggaaggtttc gggcaggacg
    13441 gcatcgaaca catacaggat ttcattgtgc acacctcggc tgacggggcg aaggctgtgc
    13501 agccgcgata ctgggcggat gagcggaaac agcgttttat ccaaaccggc ttcttcgctg
    13561 ctttcgcggc acacggcttc agacggcatt tcgccgccgg aaacaccgcc gccggcaata
    13621 ttgtcgagct tgccgggatc gactgctttg tgcggactgc gcctgcctat ccaaaaatgc
    13681 catctgccgt tcgattcgac caaaccgttg agatggacgg cgcggctgag tagtccgaac
    13741 ggacggaaag cggcgcgttc gagcgtgaac aaggggttgc cgccgccgtc ggtcaggtcg
    13801 aaacactcgt tgcgccatcc gtgaagcagc cccgccttgt tccatgtgcg ggcgaggtgc
    13861 tgcaagcgtc cgcccatatc cggccagccg tccgcattca gaaaaatgcc gtctgaagac
    13921 tcggagcagc ctgcctccca gtcttttttg atgcgctcag cccattccgg cgacagattg
    13981 cccaaaggaa gacggttcag atacagcgtt ttccagcaac tttcggcacc gtaacttgct
    14041 tttgcccgct cgaacagggc atcaaggtct tgtttgctga cggattcggt aaaacggacg
    14101 gtcggcatag ccggctttct tgaacacaat gcggaagatt gtagccaaac cgcctgaatt
    14161 tgcaaccgca aacagacggc ggcctgccgt tccgatattg cggcaggaag gaaaatccgg
    14221 caaaaaacgg aggcggcagg aaacaggaaa aacaaaaccc cctcggcatt gaaccgaggg
    14281 gtttgatatt tggttgcggg agcaggattc gaacctacga ccttcgggtt atgagcccga
    14341 cgagctacca tgctgctcca tcccgcgtca gaagatgaaa ctatacggca gattttttat
    14401 gttgtcaaac attatttccg tgaaaaatca taaagttttc cgtccggcgg cttatgcctg
    14461 atttgaaata tcattttctt tacaaaagtt catgttcgtg atttaatttt ggttaacatc
    14521 gaaacagggg tgctgcctga tgttcaggcg gctgagaaat accctttaca cccgatcggg
    14581 ataatacctg cgtggggagt tttcacggat tctgtctttc agacggcatt ggttttcaaa
    14641 tgccgtctga aaacgcaaaa cgctcctgtt tctttattct aaacgagaaa acaggagcat
    14701 tttttatgac tacgccaaaa aaaaccgcca aaatttccgg caacgaagcg cgcgaacttt
    14761 ccgacttgag cgaagacatc ggcatccgct tcaaatatcc gaactccgaa cgcgtgtatc
    14821 tgcaaggcag ccgcgacgac atccgcgtac ctttgcgcga aatccgtcag gacgacacct
    14881 acacggcgca aggtacggaa gccaacccgc ctattccggt gtacgacacc agcggcgcat
    14941 acggcgatcc ggcggcgcac atcgacctga aacaaggcct gccgcacatc cgcacggcgt
    15001 ggctggacga acgcggcgat accgaaatcc tgcccaaact ctccagcgaa tacggcatcg
    15061 aatgcgcaca cgatccgaaa accgcccatc tgcgcttcaa ccaaatcacc cgcccgcgcc
    15121 gtgccaaagc cggccgcaat gtaacccagc tccattacgc ccgccaaggc attatcacgc
    15181 ccgaaatgga gttcgccgcc atacgcgaac gcatgaagct ggacgagctt ttcagacggc
    15241 ctgaatacgc caagctcttg aaacagcaca cagggcaaag tttcggtgcg aacatcccga
    15301 cccgtcccga ccaaatcacc cccgaattcg tgcgccaaga aatcgccgcc ggacgcgcga
    15361 ttatccccgc caacatcaac caccccgaac tcgaaccgat gattatcggc cgcaacttcc
    15421 gcgtcaaaat caacggcaac ttgggcaatt ccgccgtaac ctccagcctg accgaagaag
    15481 tcgaaaaaat ggtgtggtcg ctgcgttggg gcgcggacac gattatggac ttatccacag
    15541 gcgcgcacat ccacgaaacg cgcgaatgga ttatccgcaa cgcgcccgtc cccatcggca
    15601 cagtgccgat ttaccaaact ttggaaaaaa ccggcggcat cgccgaagat ttgacttggg
    15661 atttgttccg cgacacctta atcgagcagg cggaacaagg cgtggactat ttcaccatac
    15721 acgcgggcgt gttgctgcgt tatgtgccga tgaccgccaa ccgcctcacc ggcatcgtat
    15781 cgcgcggcgg ttcgattatg gcgaaatggt gtttggcaca tcatcgggaa aacttcctct
    15841 acacgcattt cgacgaaatc tgcgaaatca tgaaggcgta tgacgtatcg ttcagcttgg
    15901 gcgacggcct gcgccccggc tgcattgccg atgccaacga cgaatcccaa ttcgccgaat
    15961 tgcacacctt gggcgaattg accgataaag cgtggaaaca cgacgtacaa gtcatgatcg
    16021 aaggccccgg ccatgtgccg ctgcaacgcg tcaaagaaaa tatgaccgaa gagttgcaac
    16081 actgctttga agcaccgttt tacacactcg gcccgctcgt taccgacatc gcccccggct
    16141 acgaccacat cacttcgggc ataggcgcgg ccaatatcgg ctggtacggc acggcgatgc
    16201 tttgttacgt tacccccaaa gaacatttgg ggctgcccga caaagaagac gtgcgcaccg
    16261 gcatcatcac ctacaaactc gccgcccacg ccgccgacct cgccaaaggc tggccgggcg
    16321 cgcaattacg cgacaacgcc ctgagcaagg cgcgtttcga attccgctgg cgcgaccaat
    16381 tccgcttaag cctcgacccc gaacgcgccg agagcttcca cgacgaaacc ctgcccgccg
    16441 aaggcgcgaa aatcgcccat ttttgctcaa tgtgcggccc caaattttgc tcgatgaaaa
    16501 tcacgcagga agtgcgcgac tacgccgaca agcaaaaagc ccaacggcag ggtatggagg
    16561 aaaaagcggt cgagttcgtc aaaaaaggcg cgaagattta cagttgaaac gtcaagcaaa
    16621 aaatgccgtc tgaagcccgg aaaaaaggct tcagacggca ttttttcgct tgtgaaaatg
    16681 attaaacaac cgatcattaa agtaattttt aaaattttta tattcacata gatgggaatg
    16741 atggatttag ggttctgatt ttgttttgag agaatgaagg aatttgagat tgtgggcgat
    16801 tatcgggaaa aatagaatct ttccgccgt
//
LOCUS       DBJGGI010000046        16676 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.046, whole genome shotgun sequence.
ACCESSION   DBJGGI010000046 DBJGGI010000000
VERSION     DBJGGI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16676
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.046"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(12..1850)
                     /gene="glmS"
                     /locus_tag="ACSIGJ_001458"
     CDS             complement(12..1850)
                     /gene="glmS"
                     /locus_tag="ACSIGJ_001458"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HHQ1330299.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVYSDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGKLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSPASLEPGPHSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSVCNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVVLFGLAVTLAKVRGVVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNAAEGVHVI
                     RAPRHVGELSPVVHTVPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            2123..2644
                     /locus_tag="ACSIGJ_001459"
                     /pseudo
     CDS             2123..2644
                     /locus_tag="ACSIGJ_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2933..4258)
                     /gene="mltA"
                     /locus_tag="ACSIGJ_001460"
     CDS             complement(2933..4258)
                     /gene="mltA"
                     /locus_tag="ACSIGJ_001460"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HHQ1330300.1"
                     /translation="MKKHLLRSALYGIAAAILAACQSRSIQTFPQPDTSVINGPDRPA
                     GIPDPAGTTVAGGGAVYTVVPHLSMPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPIHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDGRRTERARFPIY
                     GIPDDFISVPLPAGLRGGKNLVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKSYMRQNPHKLAEVLGQNPSYIFF
                     RELAGSGNEGPVGALGTPLMGEYAGAIDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            4491..5310
                     /gene="efeU"
                     /locus_tag="ACSIGJ_001461"
                     /pseudo
     CDS             4491..5310
                     /gene="efeU"
                     /locus_tag="ACSIGJ_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
     gene            5307..6473
                     /gene="efeO"
                     /locus_tag="ACSIGJ_001462"
     CDS             5307..6473
                     /gene="efeO"
                     /locus_tag="ACSIGJ_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HHQ1330301.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGETQSANEGGS
                     VGIAVNDNACEPMNLTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVADSGFKDTANEADLEKLPQPLADYKAYVQGEV
                     KELAAKTKTFTEAVKAGDIEKAKSLFAATRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEHALWVEKDVSGVKETAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEAAGSKISGEEDRYSHTDLSDFQANADGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLSEADRKALQAPINALAEDLAQLRGILGLK"
     gene            6780..8045
                     /gene="efeB"
                     /locus_tag="ACSIGJ_001463"
     CDS             6780..8045
                     /gene="efeB"
                     /locus_tag="ACSIGJ_001463"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HHQ1330302.1"
                     /translation="MSQNQPAQPTKRNLFKTALAVGAIGAIGGYFGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPRQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPSAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKTVHLQEMRD
                     FPNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTAQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRRF
                     VEFWGRTPLQEQTDIFGRRKYSGTPMDGKKEADQPDFAKDPEGNTTPKDSHMRLANPR
                     DPEFLKKHRLFRRAYNYSRGPASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVGKGGFLGQGLPGV"
     gene            complement(8117..8446)
                     /locus_tag="ACSIGJ_001464"
     CDS             complement(8117..8446)
                     /locus_tag="ACSIGJ_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HHQ1330303.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAALQNGDTVILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(8521..9891)
                     /gene="glmU"
                     /locus_tag="ACSIGJ_001465"
     CDS             complement(8521..9891)
                     /gene="glmU"
                     /locus_tag="ACSIGJ_001465"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HHQ1330304.1"
                     /translation="MPQNTLNTVILAAGKGTRMYSQMPKVLHCIGGKPMVERVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDAVWVEQTEQLGTGHAVKTALPHLASEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPTGLGRIIRDGSGSVTAIVEEKDASAT
                     QKTIREINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVRPVRVRA
                     SHLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     VVIEGEVELGDNVEIGANCVIKNAKIGANSKIAPFSHLEGCEVGENNRIGPYARLRPQ
                     AKLADNVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSAITRNIEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(9946..10218)
                     /locus_tag="ACSIGJ_001466"
     CDS             complement(9946..10218)
                     /locus_tag="ACSIGJ_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HHQ1330305.1"
                     /translation="MRKTFLILTVSAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERRAVLPDQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(10211..10924)
                     /locus_tag="ACSIGJ_001467"
     CDS             complement(10211..10924)
                     /locus_tag="ACSIGJ_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HHQ1330306.1"
                     /translation="MQENPTVWLFDLDNTLHDADAGIFHLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMADTENTLCR
                     LNGRKAVFSNGPSFYVRAVAGALGLENCFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKSHTPPFIDASVSDMAQLARYAETLSER
                     RQNHYNTRTPPENTKECYA"
     gene            10983..11984
                     /locus_tag="ACSIGJ_001468"
     CDS             10983..11984
                     /locus_tag="ACSIGJ_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HHQ1330307.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSVNYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANIGGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSPEV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPAHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            12078..12926
                     /locus_tag="ACSIGJ_001469"
     CDS             12078..12926
                     /locus_tag="ACSIGJ_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HHQ1330308.1"
                     /translation="MDFKQFDFLHLISVSGWGHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSSLPLCRAQVIVGVDYNCDLKVAKEAVLKAAAEHPLSVQN
                     EERQPAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            12953..13435
                     /locus_tag="ACSIGJ_001470"
     CDS             12953..13435
                     /locus_tag="ACSIGJ_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HHQ1330309.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEYRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLVENGGDAV
                     "
     gene            complement(13519..15087)
                     /gene="msrAB"
                     /locus_tag="ACSIGJ_001471"
     CDS             complement(13519..15087)
                     /gene="msrAB"
                     /locus_tag="ACSIGJ_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HHQ1330310.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQAEKWAQDAKFSSANLITVASPGFLH
                     EKKDGEFQKWYAGLNYPKLPVVTDNGGTIAQNLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRNPNADLGSLKHSFYKPDTQKKDSAIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTENPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYYFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKAAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSFNMRRTEVRSRAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKGKVK"
     gene            15233..16486
                     /gene="ftsY"
                     /locus_tag="ACSIGJ_001472"
     CDS             15233..16486
                     /gene="ftsY"
                     /locus_tag="ACSIGJ_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HHQ1330311.1"
                     /translation="MFSFFRRKKKQETPALEEAQVQETAAKVESEVAQIVGNIKEDVE
                     SLAESVKGRAESAVETVSGAVEQVKETVAEMPSEAGEAAERVESAKEAVAETVGEAVG
                     QVQEAVATTEEHKLGWAARLKQGLAKSRDKMAKSLAGVFGGGQIGEDLYEELETVLIT
                     GDMGMEATEYLMKDVRGRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPETKEPF
                     VIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGGRNNVTVI
                     SQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKAIPGA
                     PHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRPVPVR
                     YIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         3496 a         4356 c         5070 g         3754 t
ORIGIN      
        1 accggcggga tttattcgac ggttacggat ttcgccaggt tgcgcggctt gtccacatcc
       61 gtgccgcgcg cgagggcggt gtggtaggcg aggagctgca cggggacggt atgcacgacg
      121 ggggacagtt cgccgacgtg gcgaggtgcg cggataacgt gcacgccttc ggcggcatta
      181 aaattgctgt cgaggtcggc aaagacgaaa agttcgccgc cgcgcgcgcc gacttcctgc
      241 atattggctt tgactttgtc caacaggctg tcgttgggcg cgatgacgac gacgggcatg
      301 ttttcgtcca ccaaggcaag cggcccgtgt ttcagttcgc cggcaggata ggcttcggcg
      361 tggatgtagg tgatttcctt tagcttcaac gcaccttcaa gggcaatcgg gtaatggatg
      421 ccgcgcccca aaaacagcgc gctggttttc ttggcaaact gttgcgccca agcggcaatt
      481 tgcggttcga ggttcaaagc gtgctgcacg ctgccgggaa gctggcggag ttcttcggtg
      541 taacgtgctt catcttcgcc ggataccacg ccgcgcactt tcgccagcgt taccgccaaa
      601 ccgaacagca cgaccagttg cgtggtaaat gctttagtcg aggcgacgcc gatttccgca
      661 ccggcacggg tataaagcac aaggctgctt tcgcgcggca gggcggattc catcacgttg
      721 cagacggaga ggctgtggcg gtgtcccaaa gatttggcgt atttcaaagc ctccatcgtg
      781 tccagcgttt cgccggattg ggaaatggta atgaccagtt ggtcggggtc ggcaatcacg
      841 ctgcggtacc ggtattcgct tgcgatttcg acgtcggccg ggattttggc gatggattcc
      901 aaccaatatt tggcggtcag cgcggcgtaa taggacgtgc cgcaggcaag gattttgacg
      961 ctgcggatgc tttcaaacac gcttttggca tttttgccga agttttcggg gatgaatccg
     1021 ccgtcgagga aaacttccgc cgtgtccgca atcgcgcgcg gctgctcgtg gatttctttt
     1081 tgcataaagt ggctgtgcgg acccggttcc aaagaggcgg gcgagagttc ggataccttg
     1141 actttgcgtt cggcaggcag gccgttttta tcggtcagcc gtttgatgcc gtctgaagcc
     1201 agcagcgcga tgtcgccgtc ttcgaggtac gccacgcggc gcgtaaaggc gatgacggcg
     1261 gatacgtccg aagcgataaa ggtttcatcg tcgcccaaag cgaccaaaag cgggcagccc
     1321 atacgcgcca caactaattc atcaggcttg tcttgggcaa taaccgcgat ggcgtatgcg
     1381 ccgtggaaac gtttgaccgc ttcctgcacg gcttcaaaca atttgccgcc gttttgcgcg
     1441 tattcgtgat tgatgctgtg tgcgatgact tcggtatccg tttgcgattc aaaccggtat
     1501 cccaaacctt ccaagcgttt gcgttcgctt tcaaagtttt cgatgatgcc gttgtggacg
     1561 acggcaatca taccgccgct gatgtgcggg tgggcgttcg gctcggtaac gccgccgtgt
     1621 gtcgcccagc gcgtatgccc gatgccgatg ccgccgctga tgcctttttc gcgtgccgcg
     1681 tcctccataa gctgcacgcg tccgacgcgg cgcacgcgct tgattttgcc gtctgaatac
     1741 acagcaatgc ctgatgagtc ataaccccgg tattcgaggc gtttgaggcc gtcggtcaga
     1801 aaatcgacga cgttgtgatg ggcgcggatg gcgccgacga taccgcacat aaactgttcc
     1861 ttagtatccg gttgaaaaaa aagatgcgga cggcttccgt accgcacctt ccgtttcgga
     1921 ttataaaccg cctcccgcgc cggaaaacag caaaatgccg tctgaaggct tgggcttgct
     1981 caaaaaaggg agggatttcc ccgcctgacc gggatgggcg ttcagacggc attgcctgcc
     2041 gccggtttat agtttttgca aatcaatatt aacaagctga aaaaaaaaca atatacccgc
     2101 ccggtcttaa tgttaacgga gtatggaaat gaaacaaatg cttttggccg tcggcgtggc
     2161 ggcggtgttg gcgggctgcg gcaaggatgc cggcggttac gagggttatt ggcgcgaaaa
     2221 gtcggacaaa aaagagggcg tgattgccgt caaaaaagaa aaaggcaatt acttccttaa
     2281 taaaatcaac gtgtttacag gcaaggagga gtctttgctt ttgtctgaaa aagacggcgc
     2341 gctttcgata aacacgggga taggggaaat cccgatcaaa ctttccgacg acgggaaaga
     2401 gctgtatgtc gaacgcaggc ggtatgtgaa aaccgatgcg gcgatgaagg acaaaatcat
     2461 cgcccaccag aaaaagtgcg gacaaacggc acaggcatac ctcgacgcgc gaaatgcgtt
     2521 gccgtcaaac caaacgtatc agcagcgtca ggcggcgatc gagcaattga aacggcggtt
     2581 tgaagccgag tttgacgaat tggaaaaaga aatcaaatgc aacggcagaa ccccggcatt
     2641 gttgctttgg caggggacaa ccgggaggat gccgccgtcc gaatcggacg cgtggtttcc
     2701 ataccggtac gggcgggcgg gaatgtccgc ctttttttgt tcggatgcgt ttgaataccc
     2761 gtccgattct gtccgtttgc aagggggatt tccgttcggg cggaaatcca ttccgtcaaa
     2821 taagaaaggc gttttgtccg accggccggc ttgtgcctgc ggtatcgcaa aacttcgcct
     2881 taatgcgccc gattggctag ggacgggctt cggacggcat tgttttgccg gttcacgggc
     2941 ggtattcggg cttcatgccg ttgggcagga gctgccagac gtatcctgtg gttttctgtt
     3001 tgccggcaag ttcgccggct tcgtcgccgt aaccccaaaa ataatccacg cgcaccgcgc
     3061 ctttgatcgc gctgcctgta tcctgcgcca taatcaggcg gttgagggct ttgcgggtaa
     3121 ccggatgggc ggtggcgaca aataagggcg cgcccaaggt aatgtagtgc cggtcgattg
     3181 cgccggcgta ttcccccatc agcggcgtgc ccagtgcgcc gacggggccc tcattgccgc
     3241 ttccggcaag ctcgcggaaa aagatatagc tggggttttg acccaaaact tcggcgagtt
     3301 tgtgcgggtt ttgccgcata tatgacttaa tgccctgcat ggaggtttgc ccgagcttga
     3361 ggtagccttt gtccgccata tagcgtccga tggaaacata cggatgctcg tttttgtcgg
     3421 catagccgat gcggatgtat ttgccggacg gggttttcag acggcccgag ccttgtatgt
     3481 gcatgaaaaa aagttcgacg gggtcttcgg cgtaaccgag gatgggggct ttgccgtcaa
     3541 gcgcgccgcc gttgatttgg ttgcgcgtgt ggtaagggag gaagcggctt ccttcaaacc
     3601 tgcctttgat tgccgttgtg cgcgcggtga tggggaatcg ggagaggtcg gcggtatgcg
     3661 tgccgccggc attgtcgatc gtgccgctgt ttttccccgt ctgcctgatg cggacaaggt
     3721 tttttccgcc ccgcaaaccg gcaggcagcg ggacggagat aaaatcgtcg ggaataccgt
     3781 aaatcgggaa gcgggcccgt tccgtccgcc tgccgtcgcc cttcagcacc ggttcgtaat
     3841 agccggtaac cgtgccggca aggcttccgt tgcctgcaac ctgccaaggc gtgaaatagc
     3901 gttcaaaaaa ctgttttgcc tgaaaggaat ggatgggggt ttgaaaggct tgggcacata
     3961 catcctgcca gccttggcgg tttttcaaat tggcgcagcc gaggcggaag gattgcaggc
     4021 ttttggcaaa atcctgcgcc gcccagtggg gcatggacag gtgcggcaca acggtataga
     4081 cggccccgcc gccggcaacc gtcgttccgg cggggtcggg gatgccggcc ggccggtccg
     4141 ggccgttgat gacggatgtg tcgggttgcg gaaaggtttg gatgctcctg ctttggcagg
     4201 cggcgaggat ggcggcggcg atgccgtaca gggcggagcg gagcaggtgt tttttcataa
     4261 tggacaatgt tgtcgtcagt aataaaaaaa gatggtttcg ggcggcgttg cggtagccgg
     4321 ggagagggga ttttaacaca gggcgcagcc gcagcctgcg gaactttccg ccacgcggca
     4381 atgcagataa aaataacttg catttgtatt tgcaagtaat gaaaatattc cgataatata
     4441 ttattaatca tccttgttca ttcgcgttta cgctggtcgc ttttttaatt atgttgcgcg
     4501 agggtatcga agccgcgctc atcgtcggca tcgttgccgg ttttttgaaa cagtccggac
     4561 attcaaaaac tgatgcctaa ggtctggttc ggggtcgtcc ttgcttcttt gatgtgtttg
     4621 ggcatcggat acggcatcca ttcggcaacg ggcgagattc cccggaagca gcaggagttc
     4681 gtcgtcggca ttatcggttt ggttgccgtc gcgatgctga cttgtgtggt tttatggatg
     4741 aaaaaggcgg cgcgttcgat gaagcggcag cttcgggatt ctgtacaggc ggctttgaac
     4801 cgtggcagcg gtcaaggatg ggccttggtc ggtatggcgt ttcttgccgt ggcgcgcgaa
     4861 ggtttggaga gcgttttttt cctgcttgcc gtattcaaac agagcccgac gtggcagatg
     4921 ccggccggcg cggtagcggg ggttttggct gccgccgtga ttggcgcgtt gatttatcag
     4981 ggcgggatgc gcctgaatct ggcgaagttt ttccgttgga cgggggcgtt tctgattatc
     5041 gttgccgccg gtctgcttgc cggctcgctg cgtgcgctgc atgaggcagg catttggaac
     5101 gcgcttcagg acattgtgtt ggacccatca aaatatttgc acgaagacag tccgttgggt
     5161 gtgctgctcg gcggattttt cggctatacc gaccatccgg cgcagggcgg ggccttggtt
     5221 tggctgctgt accttattcc cgtcatgatt tggtttttgc gcggcagcag gccgtctgaa
     5281 actttaaccc gtaaagagga attgaaatga gaaaattcaa tttgaccgca ttgtccgtga
     5341 tgcttgcctt gggtttgacc gcgtgccagc cgccggaggc ggagaaagcc gcgccggccg
     5401 cgtccggtga gacccaatcc gccaacgaag gcggttcggt cggtatcgcc gtcaacgaca
     5461 atgcctgcga accgatgaat ctgaccgtgc cgagcggaca ggttgtgttc aatattaaaa
     5521 acaacagcgg ccgcaagctc gaatgggaaa tcctgaaggg cgtgatggtg gtggacgaac
     5581 gcgaaaatat cgccccgggg ctttccgaca aaatgaccgt aaccctgctg ccgggcgaat
     5641 acgaaatgac ctgcggcctt ttgaccaatc cgcgcggcaa gctggtggta gccgacagcg
     5701 gctttaaaga caccgccaac gaagcggatt tggaaaaact gccccaaccg ctcgccgact
     5761 ataaagccta cgttcaaggc gaggttaaag agctggcggc gaaaaccaaa acctttaccg
     5821 aagccgtcaa agcaggcgac attgaaaagg cgaaatccct gtttgccgcc acccgcgtcc
     5881 attacgaacg catcgaaccg attgccgagc ttttcagcga actcgacccc gtcatcgatg
     5941 cgcgtgaaga cgacttcaaa gacggcgcga aagatgccgg atttaccggc ttccaccgta
     6001 tcgaacacgc cctttgggtg gaaaaagacg tatccggcgt gaaggaaacc gcggccaaac
     6061 tgatgaccga tgtcgaagcc ctgcaaaaag aaatcgacgc attggcgttc cctccgggca
     6121 aggtggtcgg cggcgcgtcc gaactgattg aagaagcggc gggcagtaaa atcagcggcg
     6181 aagaagaccg ttacagccac accgatttga gcgacttcca agctaatgcg gacggatcta
     6241 aaaaaatcgt cgatttgttc cgtccgttga ttgaggccaa aaacaaagcc ttgttggaaa
     6301 aaaccgatac caacttcaaa caggtcaacg aaattctggc gaaataccgc accaaagacg
     6361 gttttgaaac ctacgacaag ctgagcgaag ccgaccgcaa agcattacag gctcctatta
     6421 acgcgcttgc cgaagacctt gcccaacttc gcggcatact cggcttgaaa taagccgcaa
     6481 gcgttcagac ggtatttggc ggcagatacc gtctgaagtt ttatagcgga ttaacaaaaa
     6541 ccggtacggg ttgccccgcc ccggctcaaa gggaacggtt ccctaaggcg cccaagcacc
     6601 gggcggatcg gttccgtacc atttgtactg cctgcggccc gccgccttgt cctgattttc
     6661 gttaatccgc tatatccgcc atatgttgca gggcgggctt tcagaccgcc gctatcggtt
     6721 aatggaaaaa cggcgtgcag ggatgcccac cctgctgcac ggatattgaa ggaaacgcca
     6781 tgagtcaaaa ccaacccgca caaccgacca aacgcaatct gttcaaaacc gcccttgccg
     6841 tcggcgcaat cggcgcaatc ggaggttatt tcggcggcaa aaaacagggc gaaaccgccg
     6901 aacgcaccgc cgaaagccaa cactcgcccc aagcctatcc ctgctacggc gaacatcagg
     6961 caggtatcgt tacgccgcgg caggcgtttt ccattatgtg cgccttcgac gtaaccgcgc
     7021 aaagtgccaa gcagctggaa aacctgttcc gcacactgac cgcccgcatc gagtttctca
     7081 cccaaggcgg agaataccaa gacggcgacg acaaactccc gtcagccggc agcggcattt
     7141 tgggcaaagc cttcaacccc gacggattga ccgttaccgt gggggtgggc agcagcctgt
     7201 ttgacggccg gttcggactc aaagacaaaa aaacggttca tttgcaggaa atgcgcgact
     7261 tccccaacga taagctgcaa aaaagctggt gcgacggcga tttgagcctg caaatctgcg
     7321 ccttcacccc cgaaacctgc caagccgccc tgcgcgacat catcaaacac accgcccaaa
     7381 ccgccgtcat ccgctggagt atcgacgggt ggcagcctaa atccgaaccc ggcgcgatgg
     7441 cggcgcgcaa cttgttgggc ttccgagacg gcacgggcaa ccccaaggtt tccgatccca
     7501 aaaccgccga cgaggtttta tggacgggcg tggccgccaa cagcctcgac gaaccggagt
     7561 gggcgaaaaa cggcagctat caggcagtcc gccttatccg ccgctttgtc gagttttggg
     7621 gcaggacgcc gcttcaagag caaaccgaca ttttcgggcg gcgaaaatac agcgggacgc
     7681 cgatggacgg caaaaaagaa gccgaccaac cggatttcgc caaagacccc gagggtaata
     7741 ccacgcccaa agacagccat atgcgcctgg cgaatccgcg cgatcccgaa ttcctcaaaa
     7801 aacaccgcct cttccgccgc gcctacaact attctcgcgg acccgcctca agcggacagc
     7861 ttgatgtcgg gctggtgttc gtctgctatc aggcaaatct tgccgacggt ttcatcttcg
     7921 tgcaaaacct cctcaacggc gaaccgctgg aagaatacat cagccccttc ggcggcggct
     7981 atttcttcgt cttgcccggc gtgggaaaag gcggattctt gggacaaggg ctgccgggcg
     8041 tataaacctg cctgacggac aaatgccgtc cgaaccttgc caaacgggtt cggacgggat
     8101 tttactgttt ggacggtcag gcttttttga cgaattcgga ttttaaattc atcgcgctgc
     8161 cgtcgatttt gcagccgatg ttgtgatcgc cttcctgcag gcgtatgcct ttgaccttcg
     8221 taccttgttt gatcgtcatc gaactgcctt ttaccttgag gtctttgatg aggatgacgg
     8281 tgtcgccgtt ttgcagcgcc gcgccgttgg catcgcgcac ttgagccgca aggtcggcgg
     8341 cggattcggt ttcattccat tcgtgggcgc attcggggca gatgtattgt ccgccgtctt
     8401 cataggtgta ttcggaggcg cattgcgggc aagggggtaa tgacatggtt tgccgtcctt
     8461 atcggatgtt tgttttggga tgccgtctga aacctgaaac cggcttcaga cggcacggct
     8521 ttattgtttg tctttttccg gccttaccca gccttcgatg acggtttggc gggcgcgggc
     8581 gagggcgagt ttgttgtctt cgatattgcg ggtaatcgcg ctgcctgcgc ctgtggttac
     8641 tttgttgccc agtgttacag gggcaaccag tacgcagttc gaaccgatgc gcacttcgtc
     8701 gccgatgacg gttttgtgtt tgtgcacgcc gtcgtagttg gcaataatcg tgcccgcgcc
     8761 gaagttggtt ttgctgccga cttcggcatc gcctatgtag gtgaggtggt tggctttggt
     8821 gcctttgccg atggcggcgt ttttgatttc gacgaagttg ccgacgtgta cattgtcagc
     8881 cagtttggct tgcggacgca aacgggcgta cgggccgatt cggttgtttt cgccgacttc
     8941 gcagccttcg aggtgggaga agggggcgat tttgctgttt gcgccgattt tggcgttttt
     9001 gatgacgcag tttgcgccga tttcgacgtt gtcgccgagt tcgacttcac cttcgatgac
     9061 gacgttgacg tcaatcacga catcctgtcc gtgtttcaga cggccccgca aatcgaaacg
     9121 cgccggatcg cgcagggtca cgcctgcttt gagcaattct tgcgcctgtt cggtttggaa
     9181 gatgcgttcg agttcggcga gctggcgttt attgttcacg ccggcggcga ggtgggaggc
     9241 gcgcactcgg acggggcgga ctttgatgcc gtcggcaacg gctttggcga tgaggtcggt
     9301 taaataatat tcgccttggg cattgttgct ggaaaggctg ttcagccagt tttccagttt
     9361 ggcgttgggc aggacgagga tgcctgtgtt gatttcgcgg atggtttttt gggtggcact
     9421 tgcgtctttt tcttcgacaa tggcggttac gctgccgctg ccgtcgcgga tgatgcgccc
     9481 caagcctgtc gggtcggcgg gcacgtcggt caacagcccg acttcgttgc ctgcggcttc
     9541 gagcagggtt tcgagggttt caacgtcaat taaaggaacg tcgccgtaca acaccagcgt
     9601 gcggccttcg gaggcaaggt ggggcagggc ggttttgacg gcgtggccgg tgccgagttg
     9661 ttcggtttgt tcgacccaga cggcatcgcg tttgacagtg tctaaaactt gctctttgcc
     9721 gtgtccgacg acgacgcaga tgttttgcgg attcagcgcg gcggcggtgt cgataacgcg
     9781 ctcgaccatg ggcttgccgc cgatgcagtg cagcactttg ggcatttggg aatacatgcg
     9841 cgtgcctttg ccggcggcga ggatgacggt gtttaaagtg ttttgcggca tagcgttttc
     9901 ctgtgccatg ccgtctgaag cggtttcaga cggcataggg taggtttatc ggttttgaaa
     9961 ctttggtttt tgccagtgtt ggcgatgctc ttcgtcggcg ttgttgccgg tttggtcggg
    10021 cagcacggcg cggcgttcgg gacggtattg gttgtagttc atatttttcg agtagctgcc
    10081 gtcttggtaa taaacgggcg tgccggcggg atatttttga cggacggcgg ttttgccgtt
    10141 gccgtcttga taagtttccc acgcgcagcc cgacaaaagg gcggcggaga cggtcaggat
    10201 aaggaaggtt ttacgcatag cattctttcg tattttcggg gggggtgcgg gtattgtaat
    10261 gattttggcg gcgttctgac aaagtttctg cataccgtgc cagttgcgcc atatcgctta
    10321 cggaggcatc gataaagggc ggcgtgtggg attttgcacc gaaccggacg gttttcatgc
    10381 ccaatgcttt tgcctgatgg aggttgtccg cgctgtcgtc caccataatg cagcattcgg
    10441 gcggtacgtc caacaggcgg cagacattga gatacgcttg cggattgggt ttgtacagca
    10501 gtccgaaatc atccgtgccg aacagtgcgt cgaaacagtt ttccaaaccg agtgcgccgg
    10561 caacggcacg gacgtaaaac gacgggccgt tggaaaaaac cgccttgcgc ccgttcagac
    10621 ggcatagggt gttttccgta tccgccatgc cgtgcagcct ggtcaggatt gcatcgatcg
    10681 gatggctttc gcgtaaaaat tccgcaatgt cgatttcggg atggtggatt tgcagtccgg
    10741 cgagcgttgc gccgtaacgg tgccaatagt cttgacgcag gtcggacgcg gcggattcgg
    10801 agagtttgag gcggcgtgcc atatagcgcg tcatagcgcg gttgatgagg tggaagatgc
    10861 ctgcgtcggc atcgtgcagc gtgttgtcga ggtcgaacag ccacacggtc gggttttctt
    10921 gcattttgaa ccgtgaaaat ttgttagaat gttattttac agcgaataaa ggaagactcg
    10981 gaatgaaacg gaaaatttgg ctgctgccgc tgctggcggt ttcggcatac ctgcaggcgc
    11041 agacggaagt caggctggcg gtgcataagt cgttcagcct gcccaaaggg ctgattgcgc
    11101 gcttcgagcg ggcaaacgat gcgaaagtgt cgattattca ggcgggcggc gcgaacgaaa
    11161 tgctcaacaa actgattttg agccgagcca acccgattgc cgacgcggtg tacggtttgg
    11221 acaacgccaa tatcggcaag gcgcgggaaa tgggcatttt ggcggcggcg caacccgaat
    11281 ccgcccccgt cgcggtcggg ctgccttcgg ctttggcggt cgattacggc tatgtgtccg
    11341 tcaattacga caaaaaatgg tttgaaggca aaaagctgcc cctgccgcaa accctgcagg
    11401 atttgacccg ccccgaatat aaaaacctat tggtcgtgcc gtcccccgcc acatcttccc
    11461 ccggcttggg cttcctgatg gcgaacatcg gcggtctggg cgaagaaggc gcgtttaaat
    11521 ggtgggcaca gatgcggcag aacggcgtga aggtcgccaa aggctggagc gaggcgtatt
    11581 acaccgactt ttcgcacaac ggcggcgcgt atccgctggt ggtcggttat gccgccagcc
    11641 ctgcggcgga agtatatttt tccaaaggca aatacagcga gccgccgacg ggcaacctgt
    11701 ttttaaaagg cggcgtattc cgtcaggtcg aaggcgcggc ggtcttgaag ggcgcgaaac
    11761 agccgaagct ggcggcaaaa ctggtgcaat ggctgcaaag tccggaagtg cagcaggcgg
    11821 ttccgtctga aatgtgggtt taccccgccg tcaaaaacac gcgcctgccc gacgtgttcc
    11881 gcttcgccca agccccggcg cacactaccg cccccgcgca gcgcgatatt gatgcgaacc
    11941 agcgcggatg ggtttcccgt tggatcagga cggttttgaa ataaaacaaa cataccgccc
    12001 cgcagggctt cagacggcat ttttacacct gtgccgatta cgccgcacgg ggcggatgtt
    12061 cgatcgagag gaaaacaatg gacttcaaac aatttgattt tttacacctg atcagtgttt
    12121 ccggttgggg gcatctggct gaaaaggcgt gggcgttcgg gctgaacctt gccgccgcgc
    12181 tgcttatttt cttggtcggg aaatgggcgg cgaaacgcat tgtcgccgtg atgagggcgg
    12241 cgatgacgcg cgcgcaggtc gatgccacgc tgattagttt tttgtgtaat gttgccaata
    12301 tcggcttatt gattttggtg attattgccg cattgggcag attgggcgtt tccacaacat
    12361 ccgtaaccgc cttaatcggc ggcgcgggtt tggcggtggc gttgtcctta aaagaccagc
    12421 tgtccaattt tgccgccggc gcgctgatta tcctgttccg cccgttcaaa gtcggcgact
    12481 ttatccgtgt cggcggtttt gaaggatatg tccgggaaat caaaatggtg cagacttctt
    12541 tgcggacgac cgacaacgaa gaagtcgtgc tgcccaacag cgtggtgatg ggcaacagca
    12601 tcgtcaaccg ttccagcctg ccgctttgcc gcgcccaagt gatagtcggc gtcgattaca
    12661 actgcgattt gaaagtggcg aaagaggcgg tgttgaaagc cgccgccgaa caccccttga
    12721 gcgttcaaaa cgaagagcgg cagcccgccg cctacatcac cgccttgggc gacaatgcca
    12781 tcgaaatcac attatgggct tgggcaaacg aagcagaccg ctggacgctg caatgcgact
    12841 tgaacgaaca agtggtcgaa aacctccgca aagtcaatat caacatcccg ttcccgcaac
    12901 gcgacataca catcatcaat tcttaaacgc cgtctgaaag ggggggtggg aaatggacgc
    12961 gttgcacacc atcgcccgaa acctgacgaa aaaacgtcaa accgtaagct gtgccgaatc
    13021 ctgcacgggc ggaatgcttg ccgccgcatt cacaagcgtt gcaggcagtt cgcaatggtt
    13081 cgaccagagt tttgtaacat acagcaacaa agccaaagaa taccgcttgg gcgtgttgcc
    13141 cgaaaccctg ctcgaacacg gcgcggtcag ccgccaaacc gtctatgaga tggcgcgcgg
    13201 cgcgaaagcc gtggctcagg cggattacgc cgtcggtatt tccggcatcg ccggcccggg
    13261 cggcggcgaa agcaaacccg tcggcacggt ttggttcggg tttgcctttc cgggcggaag
    13321 ttgcgaagca atgcgccgtt ttgacggcaa ccgcgaatcc gtccgcgcgc aggcggtcgc
    13381 cttcgcgttg gaacggttgg ccgggctggt tgaaaacggc ggcgatgctg tctgaacaaa
    13441 tccccgccgg ataaaaaatg ccgtctgaaa cgttgcaggt ttcagacggc attttgccgg
    13501 gttaggcggc ggtgcggctt atttcacttt gcccttcaac gcgccgtagc ctgccgcgtc
    13561 catttgttcc agcgggatga atttcaagct cgcgccgttg atgcagtagc gcagtccgcc
    13621 tttgtcgcga gggccgtcgg ggaagacgtg tcccaagtgc gaatcggcgg cgcggcttct
    13681 gacttcggtg cggcgcatat tgaagctgaa atcatcgtgt tcggtaacgg attttgcatc
    13741 aatcgggcgc gtgaagctcg gccagccgca gccggaatca tatttgtcgg cggagctgaa
    13801 caggggttcg ccgctgacaa cgtccacata aatgccgggt ttgaacaaat ggtcgtattc
    13861 gtggctgaag gcgtattcgg tcgcgctgtt ttgggtcact tggtattgct cttcggtcag
    13921 ggtgcgtttg agttcggcgt cactcggttt tttatacgtt gccgcgtcga agcctttgcc
    13981 ttgcggtgcg gctttggttt tgcccggcag cggttcgtcg gctttgcgga tgtcgatgtg
    14041 gcagtagccg ttggggtttt taatcaggta gtcctgatgg tattcctcgg cgtcgtagaa
    14101 gtttttcagc ggctcgtttt caacaacgag gggcagttgg tatttttgct gctcgcgttt
    14161 gagggcggcg gcgatgacgg ctttttcggc ggggtcggtg tagtacacgc cgctgcggta
    14221 ttgcgtgccg gtgtcgttac cctgtttgtt gaggctggtc ggatcaacga cgcggaaata
    14281 atattgcagg atgtcgtcca ggctgagttt gtcggcatcg taggtcactt tgacggtctc
    14341 ggcatggccc gtatggcggt aggacacgtc ttcgtagctc gggttttccg tgttgccgtt
    14401 ggcgtagccg gataccgcgt caaccacgcc gtcgatgcgt tggaaatagg cttccaagcc
    14461 ccagaagcag ccgccggcga ggtagatggt gcgcgtgttc atgattgctg aatccttttt
    14521 ctgagtgtcg ggtttgtaga acgaatgttt caaactgccc aaatcggcat tcgggttgcg
    14581 gattaacgcc aatgcctgcg cttcgttgat gctgcctttg acgatgcgct gcacgtcgcc
    14641 gtctttaccg attaacgccc aagaaggata aacgctgata ttcaggtttt gggcgatcgt
    14701 gccgccgttg tcggtaacga cgggcagctt ggggtagttc aaaccggcat accatttttg
    14761 aaactcgccg tcttttttct cgtgcaaaaa gccgggggag gcgacggtaa tcaggttggc
    14821 ggagctgaat tttgcatctt gcgcccattt ctcggcctgt cccaattcgg acaaacataa
    14881 aggacaccag ctcgcccaaa atttaatcag cgtcggtttg tcttttttca aataaacact
    14941 ggcggggcgg ttgtccgcgg tttttaacgt ggataaagtg tgcggcacgg tcgcggcccc
    15001 ggcatcgacg attttgggcg aacaagcgcc cagcgcaagc aggcagccga acttggcgca
    15061 aagggaaaag aaagtacggt gtttcatttt gatgtttcct gtgtggacgg tttgcatgat
    15121 tagacgtttg agatgccgaa accttacggc ccggattttc agacaacctt gccgcgcaaa
    15181 atacgctaca atacgcccta tttcaagttt ctaaaattaa aaggaaaatt caatgttcag
    15241 cttcttccgt cgcaagaaaa aacaggaaac gccggctctc gaggaggccc aagttcagga
    15301 aaccgcagca aaagtagaat ctgaagttgc tcaaatagtt ggaaatatta aagaagatgt
    15361 cgaatcttta gcagaaagcg tcaaagggcg ggccgaatct gccgttgaaa ccgtcagcgg
    15421 tgcggttgaa caggtaaagg aaaccgttgc cgagatgccg tctgaagcag gggaagcggc
    15481 ggaacgcgtt gaatccgcaa aagaagctgt tgccgaaacc gtcggcgagg ctgtcgggca
    15541 agttcaagaa gccgttgcga caactgaaga acacaagctc ggttgggctg cgcgtttgaa
    15601 acaaggcttg gccaaatcac gcgacaaaat ggcgaaatcg ctggccggcg tgttcggcgg
    15661 cggacaaatc ggcgaggatt tgtacgaaga gctggaaacc gtgctgatta ccggcgatat
    15721 gggcatggag gccaccgaat acctgatgaa agacgtgcgc ggccgcgtca gcctcaaagg
    15781 gctgaaagac ggcaacgaat tgcgcggcgc gttgaaagaa gccttgtacg acctgattaa
    15841 gccgttggaa aaaccgctgg tcttgcccga aactaaagag cctttcgtga ttatgcttgc
    15901 cggtatcaac ggcgcgggca aaaccacgtc tatcggcaaa ctcgccaaat atttccaagc
    15961 gcagggcaaa tccgtattgc tggcggcggg cgataccttc cgcgccgccg cccgtgagca
    16021 gcttcaggct tggggcgggc gcaacaatgt aaccgtcatt tcacaaacca cgggcgattc
    16081 cgccgccgtg tgcttcgatg ccgtccaagc cgccaaagcg cgcgggatcg acatcgtgct
    16141 tgccgacacc gccggccgcc tgcccacgca gcttcatttg atggaagaaa tcaaaaaagt
    16201 gaagcgcgtg ctgcaaaaag ccattcccgg cgcgccgcac gaaattatcg tcgtactcga
    16261 tgccaatatc gggcaaaacg ccgtcaacca agtcaaagcc tttgacgacg cattggggct
    16321 gacggggctt atcgttacca aactcgacgg cacggcaaaa ggcggcatcc tcgccgcgct
    16381 tgcttccgac cgccccgtcc ccgtccgcta catcggcgtg ggcgaaggca tagacgacct
    16441 gcgcccgttt gacgcgcgtg cgtttgtgga cgcactgctg gattgagccg aaatgccgtc
    16501 cgaaaacggc agaccgaacc gtcattccca caaaagtggg aatccaggac gtaaaatctc
    16561 aagaaacctt ttttcccgat aagtttccgc accgaccggc ctagattccc gcgcgggcgg
    16621 gagtccggac cgcttgttcg gcaaatgagg gggcgggttg cgcgcctgtc agataa
//
LOCUS       DBJGGI010000047        16556 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.047, whole genome shotgun sequence.
ACCESSION   DBJGGI010000047 DBJGGI010000000
VERSION     DBJGGI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16556
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.047"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            193..2220
                     /locus_tag="ACSIGJ_001473"
     CDS             193..2220
                     /locus_tag="ACSIGJ_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HHQ1330312.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSGLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITVGAPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFGVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLGLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSLKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVRIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(2309..3862)
                     /locus_tag="ACSIGJ_001474"
     CDS             complement(2309..3862)
                     /locus_tag="ACSIGJ_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HHQ1330313.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPAGETAEGDAFFENVRRFPEKPDL
                     DRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGEDEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            3913..4041
                     /locus_tag="ACSIGJ_001475"
     CDS             3913..4041
                     /locus_tag="ACSIGJ_001475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330314.1"
                     /translation="MPSENGRRQFIIFAFRRHIKRRLCAACRRFAPVLPMSDLNAV"
     gene            complement(4114..5307)
                     /locus_tag="ACSIGJ_001476"
     CDS             complement(4114..5307)
                     /locus_tag="ACSIGJ_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HHQ1330315.1"
                     /translation="MNPKHFIAFSALFAATQAEALPVASVSPDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGETFRILQIGDSHTAGDFFTDALRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHSGNWQSFTSRNNTGDFPLGGILAQTGSGGGMTLTASDG
                     KTGKQRVSLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLAIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILSYGTNEAFNNNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVLLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAQGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(5304..6287)
                     /gene="patB"
                     /locus_tag="ACSIGJ_001477"
     CDS             complement(5304..6287)
                     /gene="patB"
                     /locus_tag="ACSIGJ_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan O-acetyltransferase PatB"
                     /protein_id="HHQ1330316.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYALSDGIKTFLSGETPPTAQDGGSADMPPEAAASEAAPPAGGT
                     EWKQGTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESANLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKVKLDGQMRYLDKLLSEHLKGKIILIPTAQTLSGGKGR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAEKIMEKIVFEPSTQPSSTQP"
     gene            complement(6298..7734)
                     /locus_tag="ACSIGJ_001478"
     CDS             complement(6298..7734)
                     /locus_tag="ACSIGJ_001478"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HHQ1330317.1"
                     /translation="MPLLSVEFALFFIVFLPIYWGFAKYPSVQNLLLLAAGMGWLYHI
                     SPVFAAIIVLYSSCVYLLGELLRSDRESTRRFWLGCGIAASITVLGFFKYFDFFRPLI
                     AQYAGKGGAIDILMPLGLSYYTFQSVAYLVYCFRAPHAARFGWHELLLHLSFFPTVTS
                     GPIIRAAAFKSTDGEQAGALAQIRTRRPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALALLNTGDRYFGRDALCRLKYLAPLSWFVTFHFVCLSFVVFNTANPD
                     DAGAVFSALFANAGGWNAPQRADMLLLASFASLMLLYPYLQRAFDGTVKGLEKIPMWL
                     WFIQISIILLLIIVLAPSGIPGFIYANF"
     gene            complement(7864..9029)
                     /locus_tag="ACSIGJ_001479"
                     /pseudo
     CDS             complement(7864..9029)
                     /locus_tag="ACSIGJ_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
     gene            9470..9682
                     /locus_tag="ACSIGJ_001480"
     CDS             9470..9682
                     /locus_tag="ACSIGJ_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HHQ1330318.1"
                     /translation="METLLLDIVGMSCGGCVKSVTRILESVKGVASVEVSLENKSATV
                     GYDPAQTAAEALIEAVEDGGYDAALK"
     gene            complement(9661..9822)
                     /locus_tag="ACSIGJ_001481"
     CDS             complement(9661..9822)
                     /locus_tag="ACSIGJ_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330319.1"
                     /translation="MSEGTIARKFNQFILPVQLNQMPPEGAERASDGIGETQRLQTAF
                     LPLYFNAAS"
     gene            9830..11408
                     /locus_tag="ACSIGJ_001482"
                     /pseudo
     CDS             9830..11408
                     /locus_tag="ACSIGJ_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
     gene            11490..12587
                     /locus_tag="ACSIGJ_001483"
     CDS             11490..12587
                     /locus_tag="ACSIGJ_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HHQ1330320.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYVLGFGSGKVQAALGQNLLQAGLPLMQ
                     VWQENLASEGITLFANPLSPDTPARALSDGSHTRQRMAMDVFAANAIRAVRMQGPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFCWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQENIPSYAEALKREGRNPFFSA"
     gene            12701..13816
                     /locus_tag="ACSIGJ_001484"
     CDS             12701..13816
                     /locus_tag="ACSIGJ_001484"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HHQ1330321.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTAGNDGGIGIAAGLGYQFYDEDGNALP
                     ACGQSLLNPASVSIENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVPPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGKGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSFAEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            13884..14396
                     /locus_tag="ACSIGJ_001485"
     CDS             13884..14396
                     /locus_tag="ACSIGJ_001485"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HHQ1330322.1"
                     /translation="MPSERVSASDGIALEQGWDMKKPKILFVCLGNICRSPMAEYILR
                     RRAAEAGIPLETDSAGTSGWHDGEDMHRETAKILKKYGIDASGFTSRKIRQSDATAFD
                     YIIAMDGKNLSELEKTFGRRPEKIFKLTDLIPESGYDHVPDPWYMGDFEETYRLADAG
                     CRALLEKISK"
     gene            14473..14949
                     /locus_tag="ACSIGJ_001486"
     CDS             14473..14949
                     /locus_tag="ACSIGJ_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HHQ1330323.1"
                     /translation="MKTNFKQKIIEQARREDLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWADQGVLHKVAAVNGYILCSHAQHECNDHCHDHEEA
                     EAHHSAFILVCTECGAADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            14937..15863
                     /locus_tag="ACSIGJ_001487"
     CDS             14937..15863
                     /locus_tag="ACSIGJ_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HHQ1330324.1"
                     /translation="MSEVKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLAAGKTD
                     LCTPEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIQWLDIPVVEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            15928..16485
                     /locus_tag="ACSIGJ_001488"
     CDS             15928..16485
                     /locus_tag="ACSIGJ_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HHQ1330325.1"
                     /translation="MKPKFKTVLTALLLAVCLPSMAATRVLMETDMGNIRLVLDESKA
                     SKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLVQKATDKAVANESGNGLKN
                     TVGTIAMARTADPDSAAAQFFINLADNGSLDYKNGQYGYTVFGRVESGMDTVSKIARV
                     KTATLGLYQNVPVQPVKIRRVVVGQ"
BASE COUNT         3876 a         4190 c         4677 g         3813 t
ORIGIN      
        1 ttaacttcgg cacaccgccc cggcagctaa aaatcctgcg ggatcggtgt ggaatttagg
       61 gataatctag tacagcccca tttcggccgg tttgtgcatt tcgtcatccg gaagccggta
      121 ttttgccggc aggttttgac tactttgccc gatgaccggg tttgtctgtt tttaacaaag
      181 gagtgagaag gtttgtccct gtctgaattt atagaacgcc gaacgtcatt taatccgatg
      241 gttattttga cgactttgtt ttttgtgtgt gttttggtgg tattggtttt aaccgtgccg
      301 gatcaggtgc agatgtggct cgaccgggca aaagaagtca tttttaccga gttcagctgg
      361 ttttatgttt taacgttttc catttttctg ggttttctgc tgatactctc ggtcagcggt
      421 ttgggaaaca tcaggctagg acgggatgaa gatgtgccgg aattcggctt cctgtcgtgg
      481 ctggcgatgc tgtttgcggc cgggatgggc gtgggcctga tgtttttcgg cgtggcagag
      541 ccgttgatgc attatttttc ggacattacg gtcggcgcgc cggaacacag gcagcagcag
      601 gcattgctgc acacggtgtt ccattggggc gttcacgcct ggtcggtgta cggtacgatt
      661 gcattggctt tggcttattt cggtttccgc tacaaactgc cgcttgccct gcgttcttgt
      721 ttttaccccc tgttgaaaga aaaaatttcc ggaaggttcg gcgatgccat tgatattatg
      781 gcgttgcttg ctactttttt cggcatcatc accacattgg ggttcggggc ttcgcaactg
      841 ggcgccggat tgcaggaaat gggctggatt gccgaaaaca gcttcggcgt gcaggtcttg
      901 attatcgccg ccgtaatgtc cctcgccgtc gtttcggcaa tatccggcgt ggggaagggc
      961 gtgaaggtgt tgagcgagtt gaacctgggc cttgcgtttt tgctgctgtt ttttgttttg
     1021 gcggcgggcc ccactgttta cctgttgtcg gcattcggcg acaacatagg gaactacctc
     1081 ggaaatctgg tgcgcctcag tttgaaaact tatgcgtacg aacgggaaca caagccgtgg
     1141 tttgaatctt ggacggtgct ttattgggcg tggtggtgtt cttgggcgcc gtttgtgggt
     1201 ttgtttatcg cgcgcatttc aaaggggcgc accatccgcg agtttgtctt cggggttttg
     1261 ctcatccccg gcctgttcgg cgttttgtgg tttaccgtct tcggcaatac ggcgatttgg
     1321 ctgaatgacg gggttgcggg gggaatgctc gaaaagatga cctcctctcc ggaaacgctg
     1381 ctttttaaat tctttaatta cctccccctg cccgaactga cgagcatcgt cagcctgctg
     1441 gtcatttccc tgttttttgt aacttctgcc gactccggga tttatgtcct gaacaatatt
     1501 acctctcggg acaaaggctt gagcgcgcca cggtggcagg cggttatgtg gggcgtgctg
     1561 atgtctgccg ttgccgtttt gctgatgcgc tcgggcggac tcggcaacct gcagtctatg
     1621 accctgattg tttccctgcc gtttgccctg ctgatgctga taatgtgttt cagcctgtgg
     1681 aaaggcttga gtgcggataa gaaatatttt gagacccggg tcaaccctac cagtgtattt
     1741 tggacgggcg gcaagtggaa agaacggctg gtgcggataa tgagccagac gcaggagcag
     1801 gatattttaa aattcctcaa acataccgca tcgcccgcta tgcacgagtt gcaacgggag
     1861 ctttcggaag aatacggctt gagcgtccgg gtcgataaga tgtttcatca ggacgagccc
     1921 gcaatcgagt tcgtcattcg gaaagagacg atgcgcgatt ttatgtacgg gattaagtct
     1981 gtcgggcagg atgtatccga ccagttgatt aacgacggca agctgccgca tatccggcac
     2041 cagacaactt acaaacccta cgcttatttt ttcgacgggc gcgtcgggta cgatgtgcag
     2101 tatatgaaca aggacgagct gattgccgac attttgaaaa actacgaacg ttatttgatg
     2161 ttgttggatg atgtcggtca ggaactgatg gcgcacgagc aggtggaatt ggcagagtaa
     2221 gcgccttccc tccggcgttt gccaaaaaat gcggtgaatc agattcaccg catttttttg
     2281 ccggacgggg cttcggacgg cacggcgttc atttgtttcc gtcaaactgc tccttcagca
     2341 cccgtttcaa tactttgccc gtagcgttgc gcggcagccc gtctttaaag tggatctgtt
     2401 tggggatttt gaaatttgcc agcacggtac gcaggtggcg gcggatttcg tcctcgccca
     2461 aatccatacc ttccttcaat tggacgaagg cgacgatttc ctcgtcggca taacggtctt
     2521 tcacgccgat gacggcggcg gcttcgacgg catcgagttt gtagatttct tcttcgatct
     2581 cgcgcggata gacgttttga cctttggaaa taatcaaatc ttttttgcgg tcgacgataa
     2641 agataaagcc gtcctcgtct atggtaacga aatcgcccgt tttcaaccag ccgttgacga
     2701 tggtttcatc ggtggcggca ggcatattga ggtagccccg catcaccgaa ccgcccctga
     2761 cgatcagttc gcccacttcg ccgcgcggca cttcgaccaa ttcttcatcg acggctttgg
     2821 cttccaaacc gggcaggggg atgccgacgc tgcgggcttt ttgccgttcg ggcgtattga
     2881 cggcgacgac gggcgaggct tcgctcagtc cgtagccttc cagcaatttg gcgcggggga
     2941 acttggcttt aaaatcgagg atggtttgtt ccgccaaagg cgcgccgccg ctgataaaca
     3001 ggcggatgcg gttgaaccat ctgaaatacc aagggatttt cgccttgctc atcgcggtgt
     3061 aaatcgcggg tacgcccaaa aacacggttg cgcgtttgag cagggcctgt ttcaaaacgt
     3121 tggaaaaggg gaaaacggat ttgaccaaaa taatcgaaca cgccatataa atcggcagca
     3181 gtaccatagc cgtcagcgtg aagctgtgga acatcggcag gaaaacgata aagcggtcgc
     3241 gtttggaaat tttaaagatg cgttcgatgc cgttcaggtt ggcgaacagg ttggcgtaac
     3301 tgattagcgc gcctttggga tgccccgtcg tgccggaggt gtagatgatg tgtgccaaat
     3361 catttatccg gggttggcgg tccaagtcgg gtttttcggg gaagcggcgc acgttttcaa
     3421 aaaaggcatc gccttccgcc gtttcgccgg ccggccggct tttgtccgtc caaatgattt
     3481 tttcgacggg cgtttgcgcc ttcaggcccg ccaattcttt tgacaggccg gccgaggcga
     3541 acaggaagcg cgccttgcag tcattcagga tatacgcgta ttcgctgttt ttcaaaaatg
     3601 tgttcatcgg tacggcgacc gcgccgatgg cggagacggc gaaataggcg gtaataaatt
     3661 ctgtggaatt ggaaaccgcc agcgcgaccg tgtcgccgaa cttcacgccg atattttgca
     3721 gatacgccgc gacggcttcg gcttcctgct tgagcgcgcg gtaggcggtt ttttccttgc
     3781 cgtcgaacac tgccgtgccg ttgccgtttt tgcggcaggc ggcggcgagc atttcgtaga
     3841 aattggcata agtccggttc atgaaacttc ctttatggtt gcggacggcg aacgccctca
     3901 tttgaaaaca atatgccgtc tgaaaacgga aggcggcagt ttatcatctt tgccttcaga
     3961 cggcatatca aacgccgcct ttgcgccgca tgccggaggt tcgcccccgt ccttcctatg
     4021 tctgacctga atgccgtctg aatcaatatg aagatatacg tttcagacgg catccccctg
     4081 ttgtggaaat accgtccgcc cccccctgtc cgattattgc ctgattgcgg cggcgcggac
     4141 gagttcttcg aggctgtcgg caagcatttc cgccgcgcgc cggtagcctt gggcggagaa
     4201 gtgtacgccg tctttggcgg cccatccttg gttgagccag tttttcatgc tgcatatgcc
     4261 gcccattgcg ttttgccaag accaaaacat cgtctgcccc tgacgggcga cgcgccgctg
     4321 catctgttgg acttcggtca ggaggacggg gcgcgtgccg catacgccga gcgtgttttt
     4381 cagggattcg ggcgcgccga tgatgaggat gccggcggcg ggcaggctgt cgcggatttg
     4441 gcggacggta tccagccatt tttgttcggt atcggcaatg tcgatgttgt tgttgaaggc
     4501 ttcgttggtg ccgtaggaaa ggataaccaa atcggcgccg gtttgggcaa ggtcgttcat
     4561 acggtcggca cgccatttcg accactgggt caattgtgcg ccgttgatgc ccatcgcgga
     4621 aacggtaatg ccgccggcgg gattttcgat gttgatgaag ccgatgtccc acggcatttc
     4681 ggtctgtatg gccaggggca gtgccgcgcc cgtatccagt acctgccagc cgccgccgtt
     4741 ggcggagacg gtgttgccgt tgacggtcag ggtttgttcg gcgagcagcg gtttggcaaa
     4801 cagggaaacg cgctgtttgc cggttttgcc gtcagacgcg gtcagggtca tgccgccgcc
     4861 gctgccggtt tgggcgagga tgccgccgag cgggaaatct ccggtattgt tcctgctggt
     4921 gaagctttgc cagttgccgc tgtgacggac ggccgccatg cgctgccctt tgacgttggc
     4981 ggggtaaacc cagcctatgc cgccgtcgcc ccatgttttt tgcaggcgtt tgcgcagggc
     5041 gtcggtaaag aagtcgccgg cggtatgcga gtcgccgatt tgcaggatgc ggaaggtctc
     5101 gccgctgcct tgtgcgacgg atcggagttt tttcatccaa ggcgaggcgg cggcgttgcc
     5161 gtagtcggtc agcagcccgt ttgtatcggt gtagggggcg gacggggaaa cggtaacggt
     5221 gtcggggctg acggaggcga cgggcagggc ttctgcctgc gtggcggcga acagggcgga
     5281 aaatgcgatg aagtgtttgg ggttcatggc tgtgtacttg atggttgcgt actcggttca
     5341 aaaacgattt tttccattat tttttccgcc agcagttttt gtccttcggc ggtaaagtgt
     5401 atgccgtcct tactgcggta gcggacgggt ttgccgttga cgttgacgga atcggtgtag
     5461 cggcctttcc cgccgctcag tgtttgcgcg gtgggaatca ggatgatttt gcctttcaag
     5521 tgttccgaaa gcagtttgtc gaggtagcgc atctgaccgt cgagcttgac ttttttcatg
     5581 taggggatgc cgagccagac gacttggacg cggtgcgtgt gtgcggcttc aaggatgcgg
     5641 tcgacgcgtt tcaggtattc ttgcgcccat tcgtcggaag cgaatttgag gtagcgtttg
     5701 ccgacgggga aatcccacgg gtcgttcggg ccgaggaaga cggcgagtac gctgatttcg
     5761 ggatgttttt tcaaggtttc ttcaatcgtt ttcggccagt cgaagaatga gggataggaa
     5821 agccccgtgc tttgtttgct gaggttggcg gattcgatgc cgtattgctg tttcaggctt
     5881 ttttgcacga aaggcgcaac gccctgcatc agcgaatctc cggcgaaaaa gactttgtcg
     5941 ccgctgcgga cggcggcggc ttcggtgcct tgtttccatt ctgttccgcc ggccggcggg
     6001 gcggcttcgg atgcggcggc ttcaggcggc atatctgccg aaccgccgtc ttgagccgtc
     6061 ggcggcgttt cgccggacag gaaggttttg atgccgtctg aaagggcgta ggcgttttct
     6121 tgcaacgccg ctccgctccg ccaccatccg taggcggcaa gcggttcgag cgggctgttg
     6181 cggtggtagg tctgctgcca gtaggcgttg atggggtttt ggctgaacca cacggcaatc
     6241 agggcagaca tcagtatgga ggcgaaaagg gaaagaaagt ttttcatgtc caaaccctta
     6301 aaaattggca taaataaagc cgggtatccc cgaaggggcg aggacgataa tcagcagcag
     6361 gatgatggaa atctggataa accacagcca catcgggatt ttttccaaac ctttgaccgt
     6421 gccgtcgaaa gcgcgttgca ggtaagggta gagcagcatc aaggatgcaa acgaggcaag
     6481 cagcagcata tccgcccgtt gcggcgcgtt ccagccgccg gcattggcaa agagggcact
     6541 gaaaactgcg cctgcatcgt cggggtttgc ggtattgaag acgacaaagc taaggcagac
     6601 gaaatggaag gtaacaaacc acgagagcgg cgcaaggtat ttcagacggc atagcgcgtc
     6661 gcgtccgaaa taccggtcgc ccgtgttgag cagcgccagt gccgtgccgt gcagcgcgcc
     6721 ccaaatgagg aagttccagc cgtagccgtg ccagatgcct gagagtacca ttgccgccat
     6781 caggttgagc tgtgtccgca aaaagccttt tttgctgccg cccaagggga tgtagatgta
     6841 gtcgcgtatc caggtggaaa ggctgatgtg ccatttgtcc caaaatgcgc ggatattcgc
     6901 ggcacgaagc ggtgcggaga agtttttggg cagccggaag cccagcagca tcgccatacc
     6961 gataaccaaa tcggaatatc cggaaaagtc taaaaagagt tggaaggtat agccgtacac
     7021 gcctgccaac acgccccagc cgtcgaattg ggcgggattt tcaaatacgg gcgacaccca
     7081 gttttccgcc agcatccccg ccagccacca ttttttggca atgcccagta aaatcaggga
     7141 aacggcgagg gcggggcgga cgggcgaacg cggtcggcgg gtgcggattt gcgccagtgc
     7201 acccgcctgt tcgccgtccg tgcttttgaa tgcggcggcg cggataatcg ggccggaggt
     7261 aacggtgggg aaaaaactca ggtgcagcag cagctcgtgc cacccaaaac gcgcggcgtg
     7321 cggggcgcgg aagcagtaaa ccagataggc gacagactgg aaggtataat aggaaagccc
     7381 cagcggcatc aggatgtcga ttgcgccgcc ttttccggca tattgtgcaa tcagcggacg
     7441 gaaaaaatcg aaatatttga aaaagcccaa aacggtaatc gaggcggcaa tgccgcaccc
     7501 cagccaaaag cggcgcgtac tttcgcgatc ggaacggagc agttcgccca aaaggtacac
     7561 gcaggaggaa taaaggacga tgattgccgc aaatacgggg ctaatatggt agagccagcc
     7621 cataccggca gccaaaagca gcaggttttg gacggacggg tatttcgcaa agccccagta
     7681 aatcggcagg aagacgataa aaaacagtgc gaactcgaca gacagcagcg gcataaacca
     7741 tccttgggac aaaaaaatag gcgttaaaac acggcaaagc ccgttatttt aagcaggggc
     7801 ggatattcct tcaatcaaaa tgccgtctga agggcgggag gggcttcaga cggcatcgct
     7861 cggtcaggtt cggatatggt ggttcaaatt caggcagacc cgcaaacctg ccgccgccgt
     7921 cagcataccc gcaacgccga tgcaggaaat gcccgagtat tgcgtaacct aatgtcccag
     7981 cagtgcgccg ccgcctatac cgacgttgta aagctccgaa taaatcgagt tggcaaggtc
     8041 tgcggcatcg gaggcgaaat ccaaaacttt ggcaaccatc cccaagctga cgaccacaat
     8101 cgccgtcccc catacgaaca ccaacgcgta aatggcggca ggaaaatgcg ccaaaggcag
     8161 caaaagcccc gaagagaggg cgataagcga caccgcgccc gccaaaaatg cgcgcggatg
     8221 tttggcaaac catttgccga acaggtaaga cgcggcaaaa cccgccaggc cgtacagccc
     8281 cggaaccacc gtaacctgcc gcgcggagaa accgccgatt tggatgacga agggctcgat
     8341 gtagctgtat gcggtaaagt gcgccgtaat caccggcacc gttaccgcat acagcagcat
     8401 cagttttttc cgtttcaaaa gcaggggcag gctgcttaaa gaaccggtat tcacgctcgg
     8461 caggcgcggc aggcttttgg caagcactgc catcaccgcc gccgaacaca ggccgatcag
     8521 cagaaaactc gcctgccagc ccaaatattg tccgaccata cgcccgagcg gaatccccgc
     8581 caccatcgcc atcaccgtcc ccgtgctgag cagccccaat gcctgattcc cttttccgac
     8641 aagggcgatg cgtaccgcca gcgaagccgt aatggaccag aacaccgcgt gcgtcagcgc
     8701 aatccccacg cggcttgcga gtaggatttc aaaacgccac gaggcaaacg acaggatgtg
     8761 gctgacagta atacagcgca aacaggacca gcagcaggcc gcgccgttcc ataattgcgc
     8821 gtcagcagca tcagcggcag cggcagcgaa gtcagcgcga caatccacgc ataaaccgta
     8881 atcatcacac ccgtttcggt cgccgccatg ccgaagcttc gtccgatgtc gctcaaaagc
     8941 gcgatgggga tgtattcgac cgtgttgaaa ataaatgcgc cgattgccaa ggcaatgatg
     9001 ctcaaccaag gtttgtgcga agcgttcatc tcaattcctt atatgtcgca ccccgccaaa
     9061 agggggcgca ttatggtgaa ttaacaaaaa ccggtacggc gttgccccgc cccggctcaa
     9121 agggaacgat tccctaaggt gatgaagcac caggcgaacc ggttccgtac catttgtact
     9181 gcctgcggcc cgccgccttg tcctgatttt tgttaattca ctatactttt cttgtctgcc
     9241 ggacggcaaa atccaaatca gggggaagca gccgcctccc gttcggagga acactcggga
     9301 cgctgctttg aaaatcgttc catatgttga aacattgccg cctcccgatt gggcaaggtt
     9361 gccattatgt cgcaagcatc cgtgccgcac cgtttccgcg tttgcccaag ttcacgctcc
     9421 gacttacaat aacgcccgtc agattgcagt accaacgaaa ggaaaaatcg tggaaaccct
     9481 cctcctcgac atcgtcggca tgagctgcgg cggctgcgtc aaaagcgtta cccggatatt
     9541 agaaagcgta aaaggcgtgg caagcgtcga agtcagcctt gaaaacaaaa gtgcgaccgt
     9601 cggatacgac cccgcgcaaa ccgcagcgga agcactaatc gaagccgtag aagacggcgg
     9661 ctatgatgcg gcgttgaaat aaagcggcaa aaatgccgtc tgaagccttt gcgtttcccc
     9721 gatgccgtct gaagcacgct ccgcgccttc gggcggcatt tgatttagtt gaacgggcag
     9781 gatgaattgg ttaaacttgc gggcaatagt gccttcagac aaacaaaata tgccgaacaa
     9841 aaagaaaaaa gccgcctttg cgcgctgcaa cggagaacgg tgtgccgggc gtgtccgaca
     9901 tcaaaaattc atatgatgat ttgatgtacg aatccaaagc attttcccaa accgccatca
     9961 acgccttgga ggcatgcgcc cgcatgatgg gtttgaagcc tacggcagcc gcaaaagcca
    10021 aggtcttgga gctgggctgt tcgatgggca gaaacatcat cacgcaggca ctttattacc
    10081 cggatgcgga atttgtcggt atcgacttgt ccggcaggca ggttgcgcag ggcaacgcca
    10141 tcattgaaaa aatgggcttg gaaaatgtgc gtctggaaga aaaagatatt ttgaccgtcg
    10201 atgaatcatt cgggaagttc gactatatca tcgtccacgg catttggtcg cgggtgcccg
    10261 acgcagttaa agacaaaatc ttttcgattt gccggaacaa cctgaccgaa tacggcattg
    10321 cctatatttc atacaatgtt taccccggtt ggaagcggca ggagcagttg cgcgatatta
    10381 tgcagtttgc cggcagggat gcgcttggag aacccttgga agcaaggacg cggaaaggct
    10441 tggatgcgat caaggcgctg gcggaaattt tggaaaacga caagggcttg ggcggcggca
    10501 aacttccggc gattcaaaaa atattgaatc acaatactta ttatgtcgca cacgaatata
    10561 tggaaatatt caacaacccg atttacgtta acggtttcat tgaatgggca aaccgccacc
    10621 ggctggcgta tatcggggat accgatttgc atgtgtcttt tgtttcttgg atggcggagc
    10681 atacgcggga gcggattctg gcattggcag ggggtgatta tattgccaaa gaattttaca
    10741 gcgatatttt atccgaccgc caattccacc gttcgctttt atgccgtgag gaagtcgggg
    10801 atactgtcag gcgtgatgag tcggttgccg tcgaagtgat agaaagtttg aatttccgcc
    10861 cggcaagagg ggaaacaatc aactttgatg aaaacgatac cctgctttcg ggcatacgcg
    10921 atgtgatgaa aaccggagag gcgtttaaaa cggaagatgt tgcggaaaat cttgcccgcc
    10981 gatttcccgg tctggaattt gaccgcatga aaatcaattc ccagctttta ttgcaaacca
    11041 ttctcgggcg tttttctgtt tcatcagaca atgcgggcaa accatttttt gaagaccata
    11101 aaacctatgt gccggcgcgc tttacaaact atgccgccgc ctttgtggaa cacggtgcgg
    11161 gagcgtttgt ccggcctgcc aaccgttaca acgaaagcac gccctcattc ggatacgggc
    11221 atttgtacat tatgcggcaa ttgtcgcggc cgacgagcaa acaggcattg attgaaacgg
    11281 ttgccgaaaa cttgaacatc gtcagcgcca cgcccgacgg tttgacattc catccgcctg
    11341 ccgaagtgta tgtggaagaa atattggcag acttggcaga caggcatttt ctcgtttcgg
    11401 cggattgacg ggcaagtccc gacggcaatc cgtccgatat attttcagac aggaaagaca
    11461 cacactgcga aaaacggtac acgacacata tgaaccagac atttaccttg cccgatacgc
    11521 gcccgtatcc ccaaaatccg attaaaaacc acctgctgct caatgcctac cagttggcgc
    11581 acaattcgtc acaggcttcg cgcaaactct cgtccgggca gcttcaaacc gaaatcaggg
    11641 ggatgcttga gcaaaaccac tatatcaacc tttccctcgc gctgacgatg tcgcccgatg
    11701 ccggaactta tgccgcgctg ctttccagtg tgaacgcggt actcgattgc gagaaagaag
    11761 gcgaagtgca gtggttcgcc ctgccggtcg tgctggtgtc cggctgcaag aaagaacggg
    11821 caatcgagat gaagctgccg acggaggcat tgtttgcctg cctgcaaaac tatccgcacc
    11881 tgcgcgcgtt gacgcaagag acacaatggc tgccttatct tgtgcattct tccgatttga
    11941 gtgcggtcgc gccggatgag tggtggcgtg ccaaacaaaa taccgaagcg gcggcgcaac
    12001 acttacgccg tttcgccccg cgccctttgc tgttgcccga agggcagtcc gtccacgtcg
    12061 tttacgtgct gggtttcggc agcggcaaag ttcaggcggc attggggcag aaccttcttc
    12121 aggcaggctt gcccttaatg caggtatggc aggaaaatct tgcatcggaa ggcattacgc
    12181 tgtttgccaa tccgctttcc cccgataccc cggcgcgcgc gctttcagac ggcagccaca
    12241 cgcgccaacg tatggcgatg gatgtttttg cggcaaacgc gatacgcgcc gtccggatgc
    12301 agggtccgcg cgtcggcgtg gtcgctgcgg caaaggcggg cggacagatt ctattcggat
    12361 ttaatgcgac cgacggcgcg tttgaagtcg tgccgcaggt gttttgctgg cagctttcct
    12421 ttaccgataa cattgccgtc atccagcaga atttcctcga cctgatggca gaatgccgtg
    12481 tagaacacgt ttacctgctg cacaatccct tgggcgaaca ggaaaacatc ccgagctatg
    12541 cggaagcgtt gaaacgggaa gggcgcaatc cgtttttcag cgcatagtga tttgtagtag
    12601 tgataggctt tctcatttct atgagaaatc aaaataaaga gcacaacact ttttcattct
    12661 gtgttgtgcc ttgagtgaaa cgaaaggatg aattataaat atgaagattg taattgcacc
    12721 ggattcgttt aaggaaagct tgacagctca acaggtagct gaagcaataa aaagaggctt
    12781 ccaacaatcg atagcagatg tggaatgtct cctctgtcct gtcggtgatg ggggagaagg
    12841 cactgtagat gctatccgac attctcttga cctagaagaa aaatgtctcc aagtgacagg
    12901 accttttgga caaaaagaag tcatgcgcta ttttcaaaaa gaacaactag ccctattcga
    12961 agttgctgat ttggttggtc ttggaaaaat cccgctagag aaacgaaatc cattacaaat
    13021 ccaaactcgt ggtattggag aattgattcg ccacctcatt agtcaagaaa ttaaagaaat
    13081 ctatattggc gttggcggta cggccggtaa tgatggaggt attgggattg ctgctggttt
    13141 aggttatcaa ttttatgatg aggatggaaa tgccttaccc gcttgcggtc aatccttact
    13201 aaacccagct tctgtttcaa tagaaaatcg ctatgaaatt cctgaagatg ttcacattcg
    13261 tattttagca gatgttgtga gtcccttatg tggacaccaa ggtgcgacct atacgtttgg
    13321 caagcaaaaa gggctggatt ctactatgtt tgaggccgta gatcaggcaa tacaagattt
    13381 ttatgaaaaa gtcccccctg caacattaaa acttaaagga gcaggagctg gtggaggcat
    13441 cgctggtggt ttgtgtgcct ttgctcaggc aagtatcgta tctggaattg acacctgcct
    13501 ggacttgatt gactttgata agaaagtgtc agatgttgac ttggttatcg ttggtaaagg
    13561 aagactagat cgtcaaagtt tagcagggaa agcgcctatt ggtgtagcaa aaagaacccc
    13621 tgtcggagtt cctgttgtcg ctatttgcgg cagtttcgct gaagatttgc cttccctacc
    13681 atttgaaaat atccaagctg ccttttctat tttggagaaa agtgaacctt tagaagatag
    13741 tttgaaaaat gccagtcttt atttggagca tacggcttct aatattggac atttattaaa
    13801 tatgcctaag atttagccaa gccatttctt ccagatggat gttttggcag gttcttcctt
    13861 atgcggttac gattaaccgg ccgatgccgt ctgaaagggt ttccgcttca gacggcatcg
    13921 cgttggaaca gggatgggat atgaaaaaac cgaaaatcct ttttgtctgc ctcggcaaca
    13981 tctgccgttc gccgatggcg gaatacattt tgcgccgccg tgccgccgaa gcgggcattc
    14041 ccctcgaaac ggacagcgca gggacatcgg gctggcacga cggcgaggat atgcaccgtg
    14101 aaacggcaaa gatattgaaa aaatacggca ttgacgcttc aggctttacc agccgcaaaa
    14161 tccgccaaag cgatgcgacg gcgtttgact acatcatcgc tatggacggc aagaatttgt
    14221 ccgaattgga aaaaactttt ggcaggcggc cggaaaaaat attcaagctg accgacctga
    14281 tacccgaaag cggttacgac catgtccccg atccgtggta catgggtgat tttgaagaaa
    14341 catacaggct tgcggatgca ggctgtcggg cattattgga aaagatttcc aaataaagca
    14401 attgaataca aacacaaata taaaaccgcc tcctgtcgta tcggtcgttc ggacggcata
    14461 aacagggaat ttatgaaaac aaatttcaaa cagaaaatta tcgaacaggc acgcagagag
    14521 gacttgcagg taaccgcttt gcgcgagcag gtattagata ttgtcttgca gcaaagcggc
    14581 gtgattaaag cctacaatgt tttgtcacag atgcagcagc agagcgaagg cgtgcttgca
    14641 ccgcctaccg cctaccgtgc ccttgatttt tgggcggatc agggcgtttt acacaaagtg
    14701 gcggcggtca acggctatat tttgtgcagc cacgcgcagc acgagtgcaa cgaccattgc
    14761 cacgaccacg aagaagccga agcgcaccac agcgcgttta ttttggtctg caccgaatgc
    14821 ggcgcggcgg acgagcaaac cctcagccac gagtgggcgg cactgcgtgc aggcgttgcc
    14881 gaaagcggtt ttgcgctgaa agaagaacac gttgttttaa ctggaatctg taaaaaatgt
    14941 cagaagtgaa aaaaaccaaa gtccacctga tttcaggttt tctgggaaca ggcaaaacca
    15001 ccgcgctcaa aagcctgatg gcgcaaaaag acccgaacga aaaatgggtc atcatcgtca
    15061 acgagttcgg cgaaatcggc attgacggcg cggtattgag cgacaacggc atccctgtgg
    15121 cagaaatcgc cggcggctgt ttatgctgca ccgccggccc gcaaatgggc gtaaccgtgc
    15181 agaaaatgct gcgcgacgcc aaacccgacc gcctgatgat tgaggcaagc ggactggcgc
    15241 acgccgccag cgtcatcgac gaattgaaag ccaaacctct ggacagtctt ttggaaatcg
    15301 gcgccgtctt taccgtcgtc gatccgcgcc agttcatcaa tcccgattac gcgcaacagg
    15361 cgttgtataa agaccaaatc ggcatttgcg acgtattggc cgccggcaaa accgatttat
    15421 gcacccccga acagcttgcc gaatttcatg acaaagcggc aaaactgttc ccacccaaag
    15481 ccaaagtggt cgaagtccaa aacgcacaac tcgatatcca atggctcgat attcccgtag
    15541 ttgaaaaatc acgctaccgc ctcaaagccc tgccggacaa caccatgggc ttccagtcgc
    15601 aaggtttcac attccccgcc ggacgcgatt tcgacggcga aaaattgacc aacttcttca
    15661 atgatttgcc caaaatgacc gaaggactcg tccgcgccaa aggcgtgttc caagtattgg
    15721 gaacgtgggt gtggctcaac tgggtggacg ggcagtgggg cgcgaaccaa gtgtcttggc
    15781 ggcgcgattc gcgtttcgag ctgattgcca aatcgtttga cgcggattta atcgaacaaa
    15841 aacttaaaga cgcattggaa tagcgttcac gcgctttcag acggcatatg aaatgaaaat
    15901 gccgtctgaa ggctttagga ggacgggatg aaacccaaat tcaaaaccgt tttaaccgcg
    15961 ctgcttttgg cggtttgcct gccgtctatg gcggcaaccc gcgtcctgat ggaaaccgat
    16021 atgggcaata tccgtttggt tttggacgaa tccaaagcct ccaaaaccgt tgccaatttc
    16081 gtgcgctatg cccgaaaagg cttttacgac aacacgattt tccaccgcgt catcggcggc
    16141 ttcgtcatcc aaggcggcgg attgaccgag gacttggtgc aaaaggcaac cgataaggcc
    16201 gttgccaacg aatccggcaa cggcttgaaa aacaccgtcg gcactatcgc aatggcgcgg
    16261 acggcagacc ccgattccgc cgccgcccaa ttctttatca atctggcgga caacggctcg
    16321 ctcgactaca aaaacggaca atacggctac accgttttcg gcagggtcga aagcggaatg
    16381 gacaccgttt ccaaaatcgc ccgcgtcaaa accgccacgc tcggccttta tcaaaacgta
    16441 cccgtacagc ccgtcaaaat ccgtcgcgtt gttgtcgggc agtaacacgc agacagacgt
    16501 tcagacggcg tcgcccgttt cccaaaaaac gccgtttaaa gtaaaaaaat atttta
//
LOCUS       DBJGGI010000048        16342 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.048, whole genome shotgun sequence.
ACCESSION   DBJGGI010000048 DBJGGI010000000
VERSION     DBJGGI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16342
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.048"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(258..1043)
                     /locus_tag="ACSIGJ_001489"
     CDS             complement(258..1043)
                     /locus_tag="ACSIGJ_001489"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HHQ1330326.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGFHGKATAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDAWLESGDIVAANPKVLAQMLKIISAHV"
     gene            1287..2102
                     /locus_tag="ACSIGJ_001490"
     CDS             1287..2102
                     /locus_tag="ACSIGJ_001490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HHQ1330327.1"
                     /translation="MTALKPALPDYLGNIRIILTRTSHPANIGSAARAMKTMGLHRLT
                     IVTPNLMATPMTENPPVFNPDDVQSFALPEESFILASGAADVLHNAEIVATLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVPELLQAANRGEKVALVFGNETFGLSIEEVRACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTDSPMTHLQQEDHAATHEQIKGMLAHME
                     SVMDDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            2143..3012
                     /locus_tag="ACSIGJ_001491"
     CDS             2143..3012
                     /locus_tag="ACSIGJ_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HHQ1330328.1"
                     /translation="MTDIRISMNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELS
                     VRELLGERGFRYLLALDADPAPLTDYLAQRAPFDHRLGIYAEELLAFWFTNAPHTKLH
                     AYNLTVFSDGQTLGAADFVVSLNQQPYHIELACKYYGGDQVQNLRGLNPKDTLTDKAA
                     KLVQQFQLVHTSQGKATLAAQDLPENPLPASIVRGIGFFPQGFHAFEPPLNPYGWRGI
                     YIQDWAEYGFERQEARYHLLDRMVYLAPARVAETETLNATEIRRIDQGLIAVLECRPD
                     GFWHEIERIMKAV"
     gene            3048..4304
                     /locus_tag="ACSIGJ_001492"
     CDS             3048..4304
                     /locus_tag="ACSIGJ_001492"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HHQ1330329.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFRKHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETEFFG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWSEEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESPDAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQGEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERIGSEHDTRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVAKLLEQQH
                     SILDAASKLVKPQGCLVYATCSVLPEENELQIKRFLSEHPEFEPVNCAELLQNLKVDL
                     DTGKYLRLDSARHQTDGFFAAVLQRK"
     gene            4650..5078
                     /locus_tag="ACSIGJ_001493"
     CDS             4650..5078
                     /locus_tag="ACSIGJ_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HHQ1330330.1"
                     /translation="MNFALSVIAFTLASFLPVPPAGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTLQTKPAVKPKPAVDTNADSAKENEKDIAEKNGQLEEEKKKIAETERQ
                     NKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(5127..6470)
                     /locus_tag="ACSIGJ_001494"
     CDS             complement(5127..6470)
                     /locus_tag="ACSIGJ_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HHQ1330331.1"
                     /translation="MFHSVNVFTGETLYRRPDQDYAEFERRLADLKMHGRAFAQLGVT
                     ERAARLQKFAGRLEAEKERFAEMVCEEVGRCLHECRAEIVKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSRALFDLASDGIPLAGAWLDEADTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFIQLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKVPQGSGWFY
                     PATVLDRVNPACRVWREEVFGPVALILRAENEEHAICLANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(6475..7128)
                     /locus_tag="ACSIGJ_001495"
     CDS             complement(6475..7128)
                     /locus_tag="ACSIGJ_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HHQ1330332.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGALLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYSDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            7278..7568
                     /gene="gatC"
                     /locus_tag="ACSIGJ_001496"
     CDS             7278..7568
                     /gene="gatC"
                     /locus_tag="ACSIGJ_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HHQ1330333.1"
                     /translation="MALTLADVDKIARLSRLQLTAEEKEKSLQELNDIFTMVEQMQNI
                     NTDGIEPMAHPHEVALRLREDEVTETDRAAEYQAVAPEVRNRLYIVPQVIEE"
     gene            7631..9076
                     /gene="gatA"
                     /locus_tag="ACSIGJ_001497"
     CDS             7631..9076
                     /gene="gatA"
                     /locus_tag="ACSIGJ_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HHQ1330334.1"
                     /translation="MTQYTLKQAGSLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFVSPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNPEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSFEREKEDYTRDLDKPLKGVKIGLPKEYFSEGNSTDVQTA
                     LQNTIDLLKAQGAEPVEVSLPQTKLSIPAYYVLASAEAGTNLSRYDGVRYGHRAAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPSAAPKIGADTSPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            9073..10005
                     /locus_tag="ACSIGJ_001498"
     CDS             9073..10005
                     /locus_tag="ACSIGJ_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HHQ1330335.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQDAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDVLC
                     QMEDCRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPSGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAALKLDGAIGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDDMVTKMKDSLKTEARGISDGIQTEKPLRRTV"
     gene            10048..11478
                     /gene="gatB"
                     /locus_tag="ACSIGJ_001499"
     CDS             10048..11478
                     /gene="gatB"
                     /locus_tag="ACSIGJ_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HHQ1330336.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGGDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRIDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLDQAINYEAEAQIEILEDGGTVQQATMLFDPEKGETRVMRLKEDAHDYGYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRVQAAYFEEAAK
                     ESGQGKPTANWMNGELAATLNKEGMELADSPITAPRLAALVGKIADGTLSGKLAKKAF
                     EAMWAEPETSIAEIIEKHSLQQMTDTGAVEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKTSKGKANPAQVQELIKAKLA"
     gene            11570..12580
                     /locus_tag="ACSIGJ_001500"
     CDS             11570..12580
                     /locus_tag="ACSIGJ_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HHQ1330337.1"
                     /translation="MNHTVTLPDQTTFAAGDGETVLSAAARQNLNLPHSCKNGVCGQC
                     KAELASGDIQMGGHSEQALSEAEKAQGKILMCRTTAQSDININIPGCKADALPVRTLP
                     ARIESMVFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILPATDTGYAP
                     IRSILLDLIRQNSSRAAHFYWGARHQDDLYALEEAQGLACRLKNTCFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(12710..13342)
                     /gene="pdxH"
                     /locus_tag="ACSIGJ_001501"
     CDS             complement(12710..13342)
                     /gene="pdxH"
                     /locus_tag="ACSIGJ_001501"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HHQ1330338.1"
                     /translation="MDLHNIREDYSKRELSEADCADNPIEQFERWLDEAVRAEVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLELNPFAAMTFFWPELE
                     RQVRVEGRVGRLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            13402..13554
                     /locus_tag="ACSIGJ_001502"
     CDS             13402..13554
                     /locus_tag="ACSIGJ_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330339.1"
                     /translation="MHKLCPQSRSDDFIRKTAASGTISNRPTEDGILSTRPAAHAAEE
                     PPYQAS"
     gene            13563..14612
                     /locus_tag="ACSIGJ_001503"
     CDS             13563..14612
                     /locus_tag="ACSIGJ_001503"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HHQ1330340.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETRKTAAQAY
                     GQKASDGIKLQNAPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTTEQMRSLTEEGVMLEDGLAKVERIYEQGGEGANKWY
                     NIVIKEGRNREVRRIFESQGLTVSRLVRVAFGPIGLPNRLKRGQFYELNPAEVANILK
                     WADMLLPGERRRKKA"
     gene            complement(14671..16212)
                     /locus_tag="ACSIGJ_001504"
     CDS             complement(14671..16212)
                     /locus_tag="ACSIGJ_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate MFS transporter"
                     /protein_id="HHQ1330341.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLFYLALMMFGAFTIRVPADGWK
                     PEGYTVPKTQNKPVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSAGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     SIAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAARSGNPDDETAISDAYLVGEKVSGDGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
BASE COUNT         4162 a         4966 c         4037 g         3177 t
ORIGIN      
        1 attccccggt gtctgttttc tgccacagtc gatttagatt ccagcaaacc gcctttaaac
       61 aattgaaaga aaagggacaa attgcacagg gttctccgtt taaccagata cggatttcca
      121 ttcccggcaa tttcgggcat ttgaccgttt ccgccttcct gccccgccgc caataaaaat
      181 gccgtctgaa atatcggcac gatacttcag acggcatacc cgcccgtttt cccgcatttc
      241 cgggagctgg ctgaaattta aacgtgtgcg gaaatgattt ttaacatttg cgccagtact
      301 ttgggattgg cagccacaat atcgccgctt tccaaccaag cgtcctcacc cgacatatcg
      361 gtaacgatgc cgcctgcttc ttggacaatc aatgcaccgg cggcaatgtc ccacggtttg
      421 aggttgaact cgaaaaagcc gtcaaaacgt cctgttgcta cggcgcacaa atccaaagaa
      481 gccgcgcctt cgcggcggcc tccggcggtt tttgccaaaa agtctttcaa aatcgccaga
      541 tatttgtcca tcatgctttg atcgacaaca gggaaaccgg taccgatcag gcagcggttc
      601 aattcgatgc ggttggagac acggatgcgg cggtcgttaa gcaacgcgcc tttaccgcgc
      661 gaagccatgt acacgtcgtt acgttcgggg gcgtacacca aagcttcctg caacacgcct
      721 ttgtgcagca gtgccataga gatggcgtat tggggatggc cgtgaaggaa attggtcgtc
      781 ccgtcgagcg gatcgataat ccattcgtac tcggctgtag ctttgccgtg gaagccgctt
      841 tcttcgcaag tgattttgtg atgcggatag gcttctttca aagcctcaac caggatgatt
      901 tcggaattgc ggtcaacatc ggaaacaaaa tcgttgaagg ctttgctgtc ggttttgacg
      961 gcatcgaggt tacctgcggc gcgaatcatc atctgaccgg cacggcgggc ggctttaaag
     1021 gctgtattca aaaacggatt catcagattt ccttaagggt ggcataccgc cggttcggac
     1081 gtgcagtcct tcggagcggc aaaatcggag tttatctggt tggggcaaac cctgccaaat
     1141 cgggtaaaat accgcctgac gcgtgtctgc ttcaggcgca acgttaaatt tccgacgttg
     1201 ttaaagaaca gttcaaacgg catttcaccg tccgaacgaa aaagacggcg cattataccc
     1261 tattccattc cgaccgaaaa ccgaacatga ctgctctcaa acccgccctg cccgactacc
     1321 tcggcaacat ccgcatcatc ctcacacgca ccagccaccc cgccaacatc ggctctgccg
     1381 cacgcgcgat gaaaacgatg ggtctgcaca ggctgaccat cgtcaccccc aatctgatgg
     1441 caacgccgat gacggaaaat ccgcccgttt ttaatcccga tgacgtgcaa agttttgcac
     1501 tgcctgaaga aagtttcatc ctcgcttccg gcgcggcgga cgtattgcac aacgccgaaa
     1561 tcgtcgccac actggacgaa gcccttgccg acaccaccat cgcctgcgcc ctgaccagcc
     1621 gccgccgcga aatcaccgcg ccgctgcaaa ccccgcgcga tttggtgccc gaattactgc
     1681 aggccgccaa ccgcggcgag aaagtggcgc tggtcttcgg caacgagact ttcggcttga
     1741 gcatcgaaga agtccgagcg tgcaaccgac tgatgaccat caacggcaac cccgactatt
     1801 tctcgctcaa cctcgcccaa gccgtgcagg tcgtgtgcta cgaaattttc agccaaaccg
     1861 attcgcccat gacccatctt caacaggaag accacgccgc aacccacgag caaatcaaag
     1921 gcatgctcgc ccacatggaa agcgtgatgg acgacatcgg ctttttcaac cgccgcaacg
     1981 gcgagcgtct gatgcgccgt atgcagagcc tgttcggacg cgccaacacg caaaccgaag
     2041 acatcgacat cctgcgcggt tttttcaata ccgtcagcca ccgtatccat aaaaaagact
     2101 gattaaggcc gtctgaaaac attacaagct ttttcagacg gcatgactga tattcggata
     2161 agcatgaatt acgccctaga cgcattatgg tggaaactca ccagccaacc cgtccgcgac
     2221 ctcgcctcgc tgctgactgc gccgcctttg tggcaaagcg gttgcgaatt gagcgtgcgc
     2281 gaactactgg gggaacgcgg tttccgctac cttttagcat tggatgccga tcccgcgccg
     2341 ctgacggatt accttgcaca acgcgccccg ttcgaccacc gtctcggcat ttatgccgaa
     2401 gagctgctgg ctttttggtt taccaacgca ccgcacacca aactgcacgc atataatctg
     2461 accgtttttt cagacggcca aactttaggc gccgcggatt ttgtcgtttc cctcaaccaa
     2521 cagccctatc atatcgagct ggcgtgtaaa tactacggcg gagaccaagt gcagaacctg
     2581 cgcggtctca atcccaaaga cacgctgacg gacaaagccg ccaaattggt gcagcaattc
     2641 cagctggtgc atacgtcgca aggcaaagca accttagccg cacaagacct gccggaaaat
     2701 ccacttcctg cttccatcgt gcgcggcatc ggattttttc cacaaggttt ccatgctttt
     2761 gagccgccgc ttaatccata cggctggcgc ggcatctata ttcaagattg ggcggaatac
     2821 gggtttgaac gccaagaagc gcgctatcac ctgctcgacc gcatggtcta tctcgcgcct
     2881 gcgcgtgtcg ccgaaaccga aacattaaac gcaaccgaaa tccgccgcat cgaccaaggc
     2941 ttgattgccg ttttggaatg tcggccggac ggcttttggc acgaaatcga acgtattatg
     3001 aaggccgtct gaaacccttt accaacttta agagaaccca ttacattatg aacgccgcac
     3061 aactcgacca taccgccaaa gttttggctg aaatgctgac tttcaaacag cctgccgatg
     3121 ccgtcctctc cgcctatttc cgcaaacaca aaaagctcgg ccgccaagac cgccacgaaa
     3181 tcgccgaaac cgcctttgcc gcgttgcgac actatcaaaa aatcagtacc gccctgcgcc
     3241 gtccgcacgc gcagccgcgc aaagccgcac tcgccgcgct ggttctcggc agaagtacca
     3301 acatcagcca aatcaaagac ctgcttgatg aagaagaaac agagtttttc ggcaatttga
     3361 aagcccgtaa aaccgagttt tcagacggcc tgaataccgc cgcagaattg ccgcaatggc
     3421 tggtggaaca actgcaacag cattggagcg aagaagaaat cctcgctttc ggccgcagca
     3481 tcaaccaagc tgcgccgctc gacatccgcg tcaatacctt gaaaggcaaa cgcgacaaag
     3541 tattgccatt gttgcaagcc gaaagccccg atgcagaggc aacgccttat tctccttggg
     3601 gcatccgcct gaaaaacaaa atcgcgctta acaaacacga actgtttttg gacggcacgc
     3661 tggaagtcca aggcgaaggc agccagctgc ttgccttatt ggtgggcgca aaacgcggcg
     3721 aaatcattgt ggatttctgt gccggagccg gcggtaaaac cttggctgtc ggcgcccaaa
     3781 tggccaacaa aggcagaatc tacgccttcg acatcgccga aaaacgcctt gccaacctca
     3841 aaccgcgcat gacccgtgcc ggactgacca acatccaccc cgaacgtatc ggcagcgaac
     3901 acgatacccg tatcgcacga ctggcaggca aagccgaccg cgtgttggtc gatgcgccct
     3961 gctccggctt aggcacactg cgccgcaatc ccgacctcaa ataccgccaa tccgccgaga
     4021 ccgtcgccaa acttttggaa cagcaacaca gtatcctcga tgccgcctcc aaactggtga
     4081 aaccgcaagg ctgtttggtt tacgccacct gcagcgtgtt gccggaagaa aacgaactgc
     4141 aaatcaaacg tttcctgtcc gaacatcccg aatttgaacc cgtcaactgc gccgaactgc
     4201 tgcaaaacct gaaggtcgat ttggataccg gcaaatacct gcgcctcgat tccgcccgac
     4261 accaaaccga cggcttcttc gccgccgtat tgcaacgcaa ataaaccggt ttgaacaaaa
     4321 tgccgtcaga acccttttca aagcgttcgg acggcatttc atcaattaaa aatatcaaaa
     4381 accgcagatt caaccgattc aacaccaaag gcagaatcac gcgcctcatc ggattttata
     4441 cgcgcctgcc tgcaattttc tttttctttg ccaaaatata cggtagcata accatattcc
     4501 aatgatattt ttgggaatct gttttacccc aatatataaa gcaccatatt aaggcggagt
     4561 gtcttccctg ctttgacccg aacccggaaa agacaccgcc cgaaccaatc ccgattctgc
     4621 ccgggcagtc agccatcaag gaaaccctaa tgaactttgc tttatccgtc atcgcattta
     4681 ccctcgcctc tttcctgccc gtcccgcctg ccggagccgc cgtctttact tggaaagacg
     4741 gcggcggcaa cagctattcg gatgtgccga aacagcttca tcccgaccag agccaaatcc
     4801 tcaacctgcg gacgctccaa accaaaccgg cggtcaagcc caaacctgcc gtcgatacga
     4861 atgcggacag tgcgaaggaa aacgaaaagg atatcgccga gaaaaacggg cagcttgagg
     4921 aagaaaagaa aaaaattgcc gaaaccgaac ggcagaacaa agaagaaaac tgccggattt
     4981 caaaaatgaa cctgaaggcg gtgggaaact caaatgcgaa aaacaaggat gatttgatcc
     5041 gtaaatacaa taacgccgta aacaaatact gccgttaatc ggctctagcg caaacccgat
     5101 gccgcctgaa gcggcacggg gtttgtttat ttctgccagt aggttttgac gttgacgaac
     5161 tcgtacaacc cgaattcgga cagttcgcgc ccgtaaccgg aatctttgac tccgccgaaa
     5221 ggcaggcgca aatcgctgct ggtatggcgg ttgataaaca ccgatccggc ctgtattttt
     5281 tcggcaaacc gccaggcgcg ttcagtatcg gcggtataaa tgcaggcacc gagcccgaac
     5341 ggggaatcat tggcaaggca gatggcgtgt tcttcgtttt cggcgcgcaa aatcagggcg
     5401 acggggccga atacttcttc tctccagacg cggcaggcag gatttaccct gtctaaaacc
     5461 gtcgcagggt aaaaccaccc cgacccctgt ggaactttgc ctccggtcag gcacaccgcg
     5521 ccgtttgaaa cggcatcttc aacctgcccg tgaacccggt cccgcaaatc ttcgcggtgc
     5581 agcggtgcaa gcgtggtatc gggatgtttc gggtcgccca ttttcaattt ggcgcattcg
     5641 gcgagaaaca gctgaataaa gcggtcggct gcggcttcgg ttacgatgat gcgcttggcg
     5701 gcgttacacg attgccccgc atcgcggaaa cgggaataac aggcttctgc ggcggcgcgt
     5761 tccaaatcgg catcgggcat cacgataaag gcgttgctgc cgccgagctc caacacggtt
     5821 ttcttgaggt ttgcgcccgc atgtgccgca agaatgcgcc ccgtatgcgt cgaaccggta
     5881 aacgccatcg catcggtatc ttcgactgcc ttgagcgtgt ccgcctcatc cagccacgcg
     5941 cctgccagag gaataccgtc tgaagccaaa tcgaacaacg cccgactgac gcgtgccacg
     6001 ctgggcgcgg gtttgacggc gcacgcgttg cccgcgcaca tagcgggaac ggcaaaacgc
     6061 aatacctgcc agacgggata gttccaaggc atgacggcaa acaccacgcc caaaggctcg
     6121 aagcgcacct gactcaaact cgcctgcgtc gcaatggttt tgtgggcgag cagttcgggg
     6181 gcgaggcggg cgtaatagcg tatcagttcg atagacttga cgatttcggc acggcattcg
     6241 tgcaggcagc gtccgacttc ttcgcacacc atttccgcaa aacgctcttt ttccgcctcc
     6301 aaacggccgg caaatttttg cagacgcgcg gcacgttcgg ttacgcccaa ttgcgcgaac
     6361 gccctgccgt gcattttcaa atccgccagc cgccgttcaa actccgcata atcttgatcg
     6421 gggcggcggt aaagcgtttc gcccgtaaat acattgacac tgtgaaacat cgaatcaacc
     6481 tgccagttgc gggaatatcg ttttcagtcc cgacacaata atctccaccg ataccgccgc
     6541 cagcatcata cccataatgc ggtttaaaat cgtcagcccc gtcgcgccca gcaggcggct
     6601 gaccttcccg gcaacgatta aaatggcata acaaatcgca ctgaccacca aaccggccgc
     6661 gataatcagc gcgatatcgc tgtacgtttt ggctgccgaa gcgtaaataa tcacggtcga
     6721 aataccgccc ggaccgatgg tgatcggtat ggcgatgggc acgacggcaa tcgcccctgc
     6781 attgcgggcg gggcgcgctt gccccgtttc cggctgcgcg ccgagattct gcttggcggg
     6841 attgtcgttg ccgttcatca tcgaaatggc gatcagcagc accaaaatcc cgccgccgac
     6901 ctgaaacgaa ccgacgctga tgcccaaaac cttcaatagc gcaccgccga tcagcgcaaa
     6961 taccgcaatc acggcaaaca cggcgacggc ggccgtccgc gcgaccttcc tgcgctcctt
     7021 cgtgctgtgt ccgttggtca ggtcaaggta aagcgacaac gcgctaaacg gattgatcag
     7081 caccaaaaaa gccacaatca gcttgccgat ttccataccc aatcccatta tttcccctct
     7141 ttcaaacccg tgcggcaggc atccgatgct gcaaattgcc gccgcaacgg atttttccgt
     7201 tataattaaa aatccaagca atacgcccca tcatacccga acgacggtat ctttaccatc
     7261 agacaaggat gcttttcatg gcactgacac ttgccgacgt agacaaaatc gcccgactct
     7321 cccgactgca gctgactgcg gaagaaaaag aaaaatcgct tcaagaatta aacgacattt
     7381 tcactatggt cgaacagatg cagaacatca acacagacgg catcgagccg atggcgcacc
     7441 cgcacgaggt cgccctccgc ctgcgtgaag acgaagtaac cgaaaccgac cgcgccgccg
     7501 aataccaagc cgttgcgccg gaagtacgca accgcctgta catcgtaccg caagttatcg
     7561 aagaataatc cgaatacgct tcagacggca tcagcaatac cgcccgaagc cctttaagga
     7621 tggaagattt atgacccaat acacattgaa acaggcaggc agcctgttgc agtccaaaca
     7681 gatttccgcc gtcgaactgg caagcgcata ccttgccgcc attgccgaaa aaaatcccgc
     7741 cctcaacggc tacatcacca tcgaccaaga taaaaccctc gccgaagccc gtgccgccga
     7801 cgaacgcatc gcacagggca acgcttcggc acttaccggc gttcccgtcg cctacaaaga
     7861 cattttctgc caaaccggct ggcgcagcgc gtgcgcgtcc aaaatgctcg acaacttcgt
     7921 ctccccctac acggccaccg tcgtccaaaa cctgctcgac gaaggtatgg taacgctcgg
     7981 ccgcaccaac atggacgagt tcgctatggg ttcgaccaat gaaaactcat tctacggcgc
     8041 ggccaaaaac ccgtggaatc cggaacacgt acccggcggt tcgtcaggcg gttccgccgc
     8101 cgtcgttgcc gcgcgcctcg cccctgccgc gctcggttcg gacacgggcg gctctatccg
     8161 ccaacccgca tcgcactgcg gcattaccgg catcaaacct acctacggca cggtttcccg
     8221 cttcggtatg gtcgcctacg cctccagctt cgatcaagcc ggcccgatgg cgcaaaccgc
     8281 cgaagactgc gcgattctgt tgaatgcaat ggcaggtttc gaccccaaag actccaccag
     8341 cttcgaacgc gaaaaagaag actacacccg cgatttggac aaaccgctca aaggcgtgaa
     8401 aatcggcctg cccaaagaat acttcagcga aggcaacagc accgatgttc agacggcatt
     8461 gcaaaacacc attgatttgc tgaaagcaca aggcgcggaa ccggtcgaag tttccctgcc
     8521 gcaaaccaag ctgtccatcc ccgcctacta cgtcctcgcc tccgcagaag ccggcaccaa
     8581 cctttcacgt tacgacggcg tacgttacgg acaccgtgcc gcccaattcg gcgatttgga
     8641 agaaatgtac ggcaaaaccc gcgccgaagg ttttggcagc gaagtcaaac gccgcatcat
     8701 gatcggcact tacgtactgt cgcacggcta ctacgatgcc tattatctca aagcccaaaa
     8761 actgcgccgc ctcgttgccg atgactttca gacggcattt gcacggtgcg acctcatcct
     8821 cgcgccgacc gcaccctccg ccgcacccaa aatcggagcg gatacttctc cggttgaaac
     8881 ctacttgagc gacatttaca ccatcgccgt caacctcgcc ggactgcccg cattgaccct
     8941 gcccgcaggc ttcagcggcg gcggactgcc cgtcggtgta cagcttgtcg gcaactactt
     9001 cgccgaagcc aaaatcctcg gtgcggcgca tcaaatccaa ctcaacagcg attggcacgg
     9061 caaacgaccc gaatgaagca gaaccgcacc tttaccttcc ccgattttcg caccgtttac
     9121 agctatgcac ctttatatcg gctgcaacat ttaaaataca cattgcgaaa atttttcgga
     9181 aaaaaagaaa tttacgcctt cgagcagttt gtcaacgcct cccctatccg tcaggggctg
     9241 ttcctccact gcccgcagga tgcctatccg ctgctgcgcg aatttgttga caggcgtttt
     9301 aactgcaaac gccgtttaga tgcgatgacg gcagattttc tcatggcgga aaaactcttc
     9361 ggcacagacg ttctgtgcca aatggaagac tgccgcttcc atctggtctt ggcgcacctt
     9421 tcagacggca tcagcttgtg gctcaaccgc aacgacaact gcgtcgaaga aggcgcgtgg
     9481 tctttatctt tgcgcgatga agcaggcaac cggctgtata tggcgacctt cgcctttgtc
     9541 ggcacacacc tgctgacagc ctccgtacaa gggccgtcag gtgaagaagc caaagacacc
     9601 gtccgccgca taaccaaaca acttcacggc ttacgtcccc aacaactgat ggtaaccgca
     9661 ctgcaatatt tcgccgccgc attaaaatta gacggcgcga taggcattgc acaaaaacat
     9721 caagtcaaat tacgctggaa acttaaaaag cgcgtcaaaa tgaattacga cgcattctgg
     9781 caggaatacg gcgcaagttt ggaacgggac ggctactggc atcttcccca aacccccgcc
     9841 cgcaaagacc ttgccgacat cgaaagcaaa aagcgttcga tgtaccgcaa gcgttatgaa
     9901 atgctggacg atatggttac aaagatgaag gacagtctga aaacagaagc acgcggcatt
     9961 tcagacggca tccaaacgga aaaaccgctc cgccggacag tctgatgcga agactatcga
    10021 attgatattt tagagaaaga acctcctatg acttgggaaa ctgtaatcgg cttggaaatc
    10081 cacgtccaat tgaacaccaa atccaaaatc ttcagcggtg catcgaccgc attcggcgca
    10141 gaacccaacg cgcacgccag cgtagtggaa tgcgcgctgc cgggcgtact gccggtaatg
    10201 aaccgcgaag tcgttgaaaa agccatcaaa ttaggtttgg ctttggatgc aaaaatcaac
    10261 cggaaaaacg tgttcgaccg caaaaactac ttctatcccg acttgccgaa aggctatcaa
    10321 atcagccagt tggatttgcc gattgtcgaa cacggcaaat tggaaatcgt ggtcggcggc
    10381 gatgtgaaaa ccatcaacgt aacccgcgcc catatggaag aagacgcagg caaatccgtg
    10441 cacgagggct tgaacggcgc gaccggcatc gacctgaacc gcgccggcac gccgctgttg
    10501 gaagtggtat ccgaacccga aatgcgctcc gccgccgaag ccgttgccta cgccaaagcc
    10561 ttacacagct tggtaacctg gctggacatt tgcgacggca atatggcgga aggttcgttc
    10621 cgcatcgatg ccaacgtatc cgttcgcccg aaaggccaag cggaattcgg cacgcgccgc
    10681 gaaattaaaa acctcaattc cttccgtttc ttggatcagg cgattaatta cgaagccgaa
    10741 gcgcaaatcg agattttgga agacggcggc acagttcagc aggcgacgat gctgttcgac
    10801 ccggaaaaag gcgaaacccg cgtgatgcgc ctgaaagaag acgcgcacga ctacggctac
    10861 ttccccgatc ccgatttgct gcccgtcatc atttcagatg cccaaatgca aaaagccaaa
    10921 gcagaaatgc ccgagctgcc gaaagaaatg gcggcgcgtt tcgtggcgga ttacggcgtg
    10981 tccgaatacg acgcgcgcct gctcaccgcc agccgcgtgc aggctgccta ttttgaagaa
    11041 gccgccaaag agagcggaca aggcaagccg actgccaact ggatgaacgg cgaacttgcc
    11101 gctacgctga acaaagaagg catggaactt gccgacagcc cgattaccgc cccacgcctt
    11161 gccgcgctgg tgggcaaaat tgccgatggc acattaagcg gcaaactggc gaaaaaagcc
    11221 tttgaagcca tgtgggcgga accggaaacc agcattgccg aaatcatcga aaaacacagt
    11281 ttgcaacaga tgaccgatac cggcgcggtt gaagcgatgg tggacgaagt gctggcaaac
    11341 aacgccaaag ccgtggaaca gtttaaatcc ggcaacgaaa aagccctgaa tgcgattgtg
    11401 gggcaagtga tgaagaccag caaaggcaaa gccaatcccg cgcaggtgca ggagttgatt
    11461 aaagccaaac ttgcctgata agcaagatgc cgtctgaaac ccaaatccga cgttcagacg
    11521 gcattttcac agtctgacca cattaaaaca gcataaaaaa caatattcca tgaaccacac
    11581 cgttaccctg cccgaccaaa ccacctttgc cgccggcgac ggcgaaaccg ttttgtccgc
    11641 tgccgcccgt caaaacctca atctgcccca ttcctgcaaa aacggtgtct gcggacaatg
    11701 caaagccgaa ctggccagcg gcgatattca aatgggcgga cactcggaac aggctttatc
    11761 cgaagcagaa aaagcgcaag gcaagatttt gatgtgccgc accaccgcgc aaagcgatat
    11821 caacatcaac atccccggct gcaaagccga tgccctaccc gtccgcaccc tgcccgcacg
    11881 catcgaaagt atggttttca aacacgatgt cgccctcctg aaacttgccc tgcccaaagc
    11941 cccgccgttt gccttctacg ccgggcaata cattgattta ctgctgccgg gcaacgtcag
    12001 ccgcagctac tccatcgcca attcgcccga ccaagaaggc attttggaac tacacatccg
    12061 caggcgcgaa aacggtgtct gctcggaaat gattttcggc agcgaaccca aagtcaaaga
    12121 aaaaggcatc gtccgcgtta aaggcccgct cggttcgttt accttgcagg aagacagcgg
    12181 caaacccgtc atcctgccgg caaccgacac aggctacgcc cccatccgca gcatcctgct
    12241 cgaccttatc cgccaaaaca gcagccgcgc tgcccatttc tactggggcg cgcgtcatca
    12301 agatgatttg tatgccctcg aagaagcaca agggttggca tgccgtctga aaaacacctg
    12361 cttcaccccc gtattgtccc gtcccggaga gggctggcag ggaagaaaag gccacgtaca
    12421 agacatcgcg gcacaagacc accccgacct gtcggaatac gaagtgtttg cctgcggctc
    12481 tccggctatg accgaacaag caaaaaatct gtttgtgcaa cagcataagc tgccggaaaa
    12541 cttgtttttc tccgacgcat tcacgccgtc cgcatcataa ttcctcggta taaagaggat
    12601 tcgggctttc cgttcggaac acaaaaaact tcccgtccgt attttccccg tgaaaaaatg
    12661 ccgtctgaaa cctgattccg gttttcagac ggcatatgtt ttttcctgtt caaggcgaca
    12721 accgctcgcg tatccagccg ccatccagca aacggtattg gatgcggtcg tgcagcctgc
    12781 tcggcctgcc ctgccaaaac tcaatcaaat cgggaatcac aatatagccg ccccaatgcg
    12841 gcggacgcgg cacgtgcaaa ggatgtttga gtccgaccgc cgccgccttt gccaccaata
    12901 ccgccttgtt cggaatcacc tcgctctgcg cgcttgccca cgcacccaaa cggctctgat
    12961 acgggcgact ctcaaaatat tcgtccgaca acttctccgc cagccttccg acacgccctt
    13021 ccacgcgcac ctgacgctcc agctccggcc aaaaaaacgt catcgccgca aacggattga
    13081 gttccagcga acgccccttg cggctgtggt aattcgtaaa gaaaacaaaa ccttctgaat
    13141 taacctcctt caaaagcacc atacggctgt tgggcctccc gcgcccgtcg accgccgcca
    13201 cattgaccgc cgtcggctcg ttgacctcgg cgcgtaccgc ctcgtccaac caccgctcga
    13261 actgctcgat cggattatcg gcgcaatcgg cttccgacaa ttcccgtttg ctgtaatctt
    13321 cccgaatatt gtgcaaatcc atttaccgct cctcttatca tttgaaagat tctactcccg
    13381 cacgcaaacc gatttcaacc gttgcacaaa ctttgtcccc aaagccgcag cgacgatttc
    13441 atccgcaaaa ccgccgcatc aggtacaata tcgaaccgtc cgaccgagga cggcatttta
    13501 tcaacccgtc ctgccgcaca cgccgcagaa gaaccgcctt atcaggcgag ttaggaaaaa
    13561 tgatgtccaa gcaacccacc agcaaacgcc aatggcgcga cggcgcagcc ccgtctgcca
    13621 agaaaaccgc caaacctttc aaaagcaaag cccgtcccaa agacgaaacg cgcaaaaccg
    13681 ccgcccaagc ttacggacaa aaagcttcag acggcatcaa gctgcaaaac gcccccaaac
    13741 agcgcgccgc caaagccaaa aaactcgtcg tccgcaatcc caaccaaaaa attatggaac
    13801 acgcgcgcga tttgaaagaa cgccgcagcg acctgtcgcg tatggaaccc gaacgcctgc
    13861 aaaaagtgct tgcagcctcc ggcgtcggct cgcgccgcga aatggaagaa tggatcaaca
    13921 acggctgggt aacggtcaac ggcaaaaccg cgcaactggg cgacaaagtt acccccgacg
    13981 accacgttac cgtcaaaggc agcatcatca aactcaaatg ggccgaccgc ctgccgcgca
    14041 tcatcctgta ttacaaacaa gaaggcgaaa tcgtttcccg cgacgacccg caaggccgcg
    14101 tcagcatatt cgaccgcctg ccgcaggccg ccagcagccg ctgggtcgcc atcggacgct
    14161 tggacatcaa caccagcgga cttctgattc tcactacctc cggcgaactc gtccaacgtt
    14221 tcgcccaccc cagcttcgaa gtcgaacgcg aatacgccgt gcgcgtactg ggcgggctga
    14281 ctaccgaaca aatgcgcagt ctcaccgaag aaggcgtgat gctcgaagac ggcttggcaa
    14341 aagtcgaacg catctacgaa caaggcggcg aaggcgcaaa caaatggtac aacatcgtga
    14401 ttaaagaagg ccgcaaccgc gaagtgcgcc gcatattcga aagccaaggc ctcacagtca
    14461 gccgcctcgt gcgcgtcgcc ttcggcccca tcggactgcc caaccgcctc aaacgcggac
    14521 agttctacga actcaacccc gccgaagtcg ccaacatcct caaatgggcg gatatgctgc
    14581 tgccgggcga acgccgccgc aaaaaagcct gaaagtaaaa atgccgtctg aaacacctgc
    14641 tgtttcagac ggcattttat tcgggcgttt tcaggagaaa aggtcgagtg ctttgacaaa
    14701 gaccatcacc acgccgtagg cgagcggtat gacggccaat gcccaacgcc accagaccga
    14761 tatgccgtcg ccggaaactt tttcgcccac aaggtaagcg tcggatatgg cggtttcgtc
    14821 gtcggggttg ccgctgcgtg cggcggtttt gatgtctttt tcgtggtgtt tttcgtgtac
    14881 ggatttgacg gcgaggttgc acaacaaacc gataatcagc aggcccgcca taatgtacat
    14941 ggttacgctg tatgcctgtg ccgccggtat gccgctgtcg atttggcttt ggcgtatgta
    15001 attgaccagt accgggccga tgacggcggc gattgaccag gccagcagga tgcgcccgtg
    15061 aatcgcgccg acctgatagg tgccgaacag gtctttcagg taggcgggaa tggcggcaaa
    15121 tccgccgccg tacatggaaa taatcacgca aaagccgatg atgaacaggg ctttgctgcc
    15181 gccttcgccg atggagggaa cggcgaaata cagcagcgaa ccgagtacga agaagatggt
    15241 gtaggtgttt ttgcgtccga ttttgtcgga aacgctcgac cacaaaaagc gtccgcccat
    15301 attaaacagg ctcaggaggc tgacgaagcc tgccgccgca cctgcgccga ctgccgcctg
    15361 cctgcctgcg gaggtttcgg aaaagagttc ctgaatcatc acggatgcct ggcccaatac
    15421 gccgatgccg gcggttacgt tcaggcacaa tacccagaac aacagccaaa actgcggcgt
    15481 tttcatggct tgggacacgt tgacatgatt gctgctgacc ggcttgtttt gcgttttcgg
    15541 cacggtatag ccttcaggtt tccagccgtc ggcaggtacg cggatggtaa acgcgccgaa
    15601 catcatcagt gcgaggtaaa acaggcccaa tacggcaaag gtttcggcaa ccccgaccga
    15661 agcggcgttt gaaaaggcgt tcatcagtga tacggaaagc ggcgaggcca gcattgcgcc
    15721 gccgccgaaa cccataatcg ccaaaccggt cgccataccc ggcttgtcgg gaaaccattt
    15781 catcagtgtg gaaaccggcc cgatgtagcc caaacccaag cctacgccgc cgatgacgcc
    15841 gttgcccaaa tagagcagga agaggttgtg cgtacgcacg ccgaatgcgg atacgaagaa
    15901 gcccaggctg aaacagcagg cggcggcaaa tatggctttg cgcggcccta cccgttccat
    15961 ccatgtgccg aacagggcgg ccgacgcgcc cagcatcgcg agtgcgatac tgaaaatcca
    16021 acctacggtc gtcagcttcc aatctccggc cgccgattcg gttatgccga taagtttggt
    16081 cagcggcgcg ttgaatacgg aataggcgta aatctgcccg atggcaaggt gcaccgctaa
    16141 tgctgcgggc ggtacgagcc aacggttgaa acccggcttg gcaatgcttg cctcacggtc
    16201 taaaaacttc ataacatcct ctttctgtca gttgaaaaat aaaatttcat ttgcccaatg
    16261 gaaacttatt aaaaattata aaaaaatatc gggttgggtt tttatccgcc ccaagatgcg
    16321 ccgtctgaaa catttcgggt gt
//
LOCUS       DBJGGI010000049        16164 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.049, whole genome shotgun sequence.
ACCESSION   DBJGGI010000049 DBJGGI010000000
VERSION     DBJGGI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16164
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.049"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            344..2728
                     /gene="ppsA"
                     /locus_tag="ACSIGJ_001505"
     CDS             344..2728
                     /gene="ppsA"
                     /locus_tag="ACSIGJ_001505"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HHQ1330342.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLNERISAALAKLDVEDVSELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWDKMVTDADGADISVAVRSSATAEDLPDASFAGQQETFLNINGLDNVKKAMRH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYNQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVEVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRYAINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLENGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEIIRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGSVYEPHEENPMLGFRGAARYVAESFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVGEGIESVSLNPDTVIETWLYLANELNK"
     gene            2949..4208
                     /gene="rho"
                     /locus_tag="ACSIGJ_001506"
     CDS             2949..4208
                     /gene="rho"
                     /locus_tag="ACSIGJ_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HHQ1330343.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDSINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKIGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPASGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            4823..6127
                     /locus_tag="ACSIGJ_001507"
     CDS             4823..6127
                     /locus_tag="ACSIGJ_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HHQ1330344.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAARMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFIMA
                     AYSGLMSWAILKAVGKICGGLRVGKDVEREGLDLNIHGEHVE"
     gene            complement(6221..7516)
                     /locus_tag="ACSIGJ_001508"
     CDS             complement(6221..7516)
                     /locus_tag="ACSIGJ_001508"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HHQ1330345.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKAETCIIGGGLSG
                     LCTALPLAEHGHEAVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGVASAAAEAGAQIFEQSPMTRIEP
                     YQNGWLVYTPEGSVECKNVVYAVNTYVGLNPIFRPLERKAIAVSTFIIATEPLGARTK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVRIEYSWGGECDITANLVPHFGRLTSNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFEQLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(7643..8530)
                     /locus_tag="ACSIGJ_001509"
     CDS             complement(7643..8530)
                     /locus_tag="ACSIGJ_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HHQ1330346.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     NREAAEAYRQEKLAAEKAN"
     gene            complement(8530..9495)
                     /locus_tag="ACSIGJ_001510"
     CDS             complement(8530..9495)
                     /locus_tag="ACSIGJ_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HHQ1330347.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISSA
                     EQEIAIPPFTPLTTIDEDLGRLNIAVSYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIINNLLIKMGIIREPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDSRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(9511..10635)
                     /locus_tag="ACSIGJ_001511"
     CDS             complement(9511..10635)
                     /locus_tag="ACSIGJ_001511"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHQ1330348.1"
                     /translation="MTATTASSAKPYLQIQGLVKKFGDNYAVDNIDLNIYQNEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIDARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIENHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(10913..11182)
                     /gene="rpsO"
                     /locus_tag="ACSIGJ_001512"
     CDS             complement(10913..11182)
                     /gene="rpsO"
                     /locus_tag="ACSIGJ_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HHQ1330349.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(11263..11397)
                     /locus_tag="ACSIGJ_001513"
     CDS             complement(11263..11397)
                     /locus_tag="ACSIGJ_001513"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330350.1"
                     /translation="MWSDVKCRLKPEDVSVFRRHFADIAKYRAEQQKLCDNALFLFQE
                     "
     gene            complement(11387..12322)
                     /gene="secF"
                     /locus_tag="ACSIGJ_001514"
     CDS             complement(11387..12322)
                     /gene="secF"
                     /locus_tag="ACSIGJ_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HHQ1330351.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVARGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKMGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVNNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHTVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIAKEAKQKEEIVV"
     gene            complement(12326..14179)
                     /gene="secD"
                     /locus_tag="ACSIGJ_001515"
     CDS             complement(12326..14179)
                     /gene="secD"
                     /locus_tag="ACSIGJ_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HHQ1330352.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAENSLTVPLQDAGDVQKALPQLLKLFPEATLNSEGSNIVLALSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPIIQQSGLDRIVVQLPGVQDTAKAKDIIGRTAT
                     LEVRMVEDDPAKLREALEGNVPSGYELLSSGGEHPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(14391..14657)
                     /gene="yajC"
                     /locus_tag="ACSIGJ_001516"
     CDS             complement(14391..14657)
                     /gene="yajC"
                     /locus_tag="ACSIGJ_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HHQ1330353.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKAGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            14788..15906
                     /locus_tag="ACSIGJ_001517"
     CDS             14788..15906
                     /locus_tag="ACSIGJ_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HHQ1330354.1"
                     /translation="MPLTRLFLKLYALLRLFLGKNAQTAWIFHPACTGHDPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTVEAEEISDTRLALVRSSKYLNRLESCLPEDGKICRL
                     DDDTVISKNSLSAARFSAGSAVQAVDMVMKRKAWHAFCAARPPGHHAKSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NGMPDGGNMVHLPLPSGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASSCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLNPTKKRFAKPKTGQVRQPTQSDRYDINAV"
     gene            16050..>16164
                     /locus_tag="ACSIGJ_001518"
     CDS             16050..>16164
                     /locus_tag="ACSIGJ_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HHQ1330355.1"
                     /translation="MNTFFRQTAQAMTAKHIGRFPLSELDQVIDWQPIEQYL"
BASE COUNT         4075 a         4417 c         4034 g         3638 t
ORIGIN      
        1 aacatatctg ctaatctggt acagacccaa aaacaaatca tattttaaaa caaattatct
       61 caaaatcaga tttctttaaa ataaaattcc aaaaaaaaga aacaggaaaa atacggcata
      121 aaacattaac ctattcaaaa ccatctgttt tacaaaggaa atacccgaaa aaaacaatat
      181 gcctgccatc ggtttcaaac cgctacacag ccggaacccg ctatttttca gacggcgcag
      241 cggttgtccg gacagataaa aacgtgacag cggcggaacg aatcgcttaa aataagcaag
      301 cggatttgtt tcttttttaa catattttgg attggacaca caaatggccg acaactacgt
      361 aatctggttt gaaaacctgc gtatgacaga tgttgaacgc gtgggcggta aaaacgcctc
      421 gctgggcgaa atgatcagtc aactgaccga aaaaggcgtt cgcgtccccg gtggctttgc
      481 caccacagcc gatgcctacc gcgcatttct cgcacacaac ggtctgaacg aacgcatttc
      541 cgccgctctg gcaaaattgg atgtcgaaga cgtttccgaa ctggcacgcg tcggcaaaga
      601 aatccgccaa tggattttgg acacgccctt ccccgaacag ctcgatgccg aaatcgaagc
      661 ggcatgggac aaaatggtta ccgatgccga cggcgcggac atttccgttg ccgtacgctc
      721 ttccgcaacc gccgaagacc tgcccgacgc atcatttgcc ggccaacagg aaaccttcct
      781 gaatatcaat ggcttggata acgttaaaaa agcgatgcgc cacgttttcg cctccctgta
      841 caacgaccgc gccatttcct accgcgtcca caaaggcttc gaacacgaca tcgtcgccct
      901 ttccgccggc gttcaacgca tggtgcgttc cgacagcggc gcatcaggtg tgatgttcac
      961 cctcgacacc gaatccggct acaaccaagt cgtctttgtt acctcctctt acggtctggg
     1021 cgaaaacgtc gtacaaggcg cggtcaaccc ggacgaattt tatgtgttca aacccacgct
     1081 caaagcaggc aaacccgcca tcctgcgcaa gaccatgggt tcgaaacaca tcaaaatgat
     1141 tttcaccgac aaagccgaag ccggcaaatc ggtaaccaac gtcgaagtcc ccgaagaaga
     1201 ccgcaaccgc ttctccatta ccgacgaaga aatcaccgag ttggcacact acgcgctgac
     1261 catcgaaaaa cactacggcc gcccgatgga tatcgaatgg ggacgcgacg gcctggacgg
     1321 caaactctac atcctgcaag cccgccccga aaccgtcaaa tcccaagaag aaggcagccg
     1381 caacctgcgc cgctacgcca tcaacggcga aaaaaccgtc ttgtgcgaag gtcgtgccat
     1441 cggtcaaaaa gtcggtcagg gcaaggtgcg cctgattaaa gatgcttccg agatggattc
     1501 cgtcgaagcc ggcgacgtac tggttaccga catgaccgat ccggattggg aacccgtgat
     1561 gaaacgtgct tccgccatcg tgaccaaccg cggcggacgc acctgccacg ccgccatcat
     1621 cgcgcgcgaa ctgggtattc ctgccgtcgt cggctgcggc aatgcgaccg aactcttgga
     1681 aaacggtcag gaagttaccg tatcctgtgc cgaaggcgat accggcttta tctatgccgg
     1741 cctgctggac gtacaaatta ccgatgtcgc cttggacaat atgccaaaag cacccgtcaa
     1801 agtcatgatg aacgtcggca atcccgaact cgcattcagc ttcgccaatc tgcccagcga
     1861 aggcatcggc ttggcgcgta tggaatttat catcaaccgc caaatcggca tccacccgaa
     1921 agccttgttg gaatttgaca aacaagacga cgaactgaaa gcggaaatta tccgccgcat
     1981 cgccggttac gcatcccctg tcgacttcta cgtcgataaa atcgccgaag gcgtggcaac
     2041 gcttgccgca tcggtttatc cgcgtaaaac catcgtccgt atgtccgact tcaaatccaa
     2101 cgaatacgcc aacctggtcg gcggcagcgt atacgaaccg catgaagaaa acccgatgtt
     2161 gggcttccgt ggtgcggcgc gttatgttgc cgagagcttc aaagactgtt tcgccttgga
     2221 atgcaaagcc ttgaaacgcg ttcgtgacga aatgggtctg accaacgtcg aaatcatgat
     2281 tccgttcgtc cgcactttgg gcgaagccga agcagtggtt aaggcattga aagaaaacgg
     2341 cttggaacgc ggcaaaaacg gcctgcgcct gattatgatg tgcgagctgc cgagcaacgc
     2401 ggtattggcg gaacaattcc tgcaatactt cgacggcttc tccatcggct cgaacgacat
     2461 gacccagctg accctcggcc tcgaccgaga cagcggcttg gtgtccgaat cgtttgacga
     2521 acgcaaccct gccgtcaaag tgatgctgca tcttgccatc tccgcctgcc gcaagcagaa
     2581 caaatatgtc ggtatctgcg gtcaaggtcc gtccgaccat ccggactttg ccaaatggct
     2641 ggttggagaa ggcatcgaaa gcgtgtctct gaacccggat accgtcatcg aaacttggct
     2701 gtatttggcg aatgaattga acaaataatc aattcccata cccccaagcc tgaaaagcgc
     2761 gggggtattt tttccaagca gctccgttca gacggcattt cctgtcgatg ccccgtccgc
     2821 gataatattt gacacccacg cgccgactgc ctacaattcc cccctctccg agcaaccggc
     2881 aacagtcagc ttcttctttc agacggcatc cacccgtctt ttcctttcta ttctatatat
     2941 cattgattat gcacgtctcc gaattacaaa ccctgcacat ttccaaactc ttagaattgg
     3001 cggaagaaca cggcatcgaa aacgccaacc gattccgcaa acaagacctc gtatttgcca
     3061 tcgtccgcca gatgatgaaa aaaggcgagg gttttacctg ctccggcacg ctcgaaatcc
     3121 tgcccgacgg cttcggcttc ctccgcagcg cggacacgtc ctatcttgcc ggccccgacg
     3181 acatctatgt ctcgcccact caaatccgcc gcttcaacct gcatacgggc gacaccatcg
     3241 aaggcagcgt gcgcgtcccc aaagacaacg aacgctattt tgccttggtc agactggatt
     3301 ccatcaatgg cgaccacccg gaagtatgcc gccataaaat cctgtttgaa aacctgaccc
     3361 cgctgtttcc gaccgaacag ttgaagctgg aacgcgactt aaagtccgaa gaaaacctga
     3421 ccggacgcgc catcgacctg atttcgccca tcggcaaagg tcagcgcgcc ctcttggttg
     3481 ccccgcccaa aatcggtaaa accgtgatgc tgcaaaacat tgcccacgcc gttaccgcaa
     3541 actatcctga agtcgaactc atcgtcctct tgattgacga acgccccgaa gaagtaaccg
     3601 aaatgagccg ttccgtccga ggtgaagtag tctcctccac ctttgacgaa ccggcgcaac
     3661 gccacgtcca agttgccgaa atggtgcttg aaaaagccaa gcgtatggtg gaacacaaaa
     3721 aagacgtggt catcctgctg gattcgatta cccgccttgc ccgcgcctac aataccgtcg
     3781 tccccgcctc gggcaaaatc ctgaccggcg gtgtcgatgc caacgcgctg caccgcccca
     3841 aacgtttctt cggtgcggcg cgcaacgtgg aagaaggcgg ttcgctgacc attatcgcca
     3901 ccgcattggt tgaaaccggc agccgtatgg acgatgtgat ttacgaagaa ttcaaaggta
     3961 ccggcaatat ggaattgcac ctcgaccgcc gtatggcgga aaaacgcctc ttccccgcca
     4021 tcaacatcaa caaatccggc acgcgccgcg aagagctgct tgtgccgaac gaccagttgc
     4081 aacgtatgtg gttactgcgc aagttcctgc acccgatgga cgaaatcgaa gctaccgagt
     4141 tcttaaacgg aaaaatcaaa gcatctaaaa ataatgatga tttctttgaa ctgatgcgcg
     4201 gaaaataaac gcgccgcccg cattaatgcg aaatgccgtc tgaagcctga aaatcgggtt
     4261 tcagacgaca ttttcattcg cacggccggc gcagctcccc cccccgctaa cggcgcaacc
     4321 gccggcagtg tccgtgtccg cctgccgaag gcgcggcctt tgcaaaaccg gctcgaacgc
     4381 atccctaccg gcaatgaaaa ccgatgcggg aacttgattc aaggttgcgc cgaaccgcgc
     4441 ccatttttgt ataaatttta ttttttattt ccaattagaa atttttaata tattaatcaa
     4501 taaattaatt ttataaaata aaaatattgt caacaatctt ttgacttatc gcccaaacct
     4561 ctgtatattt tcctacagta aattgttgac aatccatacg cccgcatatg cgccgcctaa
     4621 ggataaatcc tcccgccgga caacgggggc aagggatagg atgcgatatt tccatattca
     4681 aacaagggat ttgtttcacg cacagggcgg cacatcggca aaatccccgc gccggtccgg
     4741 cagggcttgc gcccatcccg gacaagcccc gaccccgcct ttccgaaaga cgggcccaac
     4801 cattaaggaa actttaatca aaatgaaaaa acacatatgg gcggcatctt tgctgccggc
     4861 atccctatcg gcagaacctt taaactggtg gaaaccttat tccgccgtca attcgggcga
     4921 taccgcctgg gtgatgactg cggctgcctt ggtactgttg atgacgcttc ccgggctggc
     4981 tttattctac ggcggtatgg tgcggaaaaa aaacctgctc tcgacgatga tgcacagctt
     5041 ttccatcgcg acattggtgg gcatcctttg ggtcgccgtc ggctattctt tggcgttcac
     5101 gccgggaaat gcctttatcg gcggtttggg gcgcgtattt ttaagcggga tgcagataga
     5161 cgctgccgca cggatgctga ccgtgtcgcc caatgcgccg actgttcccg aaccggtatt
     5221 tatgtttttt cagatgacgt ttgccattat ttcgactgcc attattaccg gcgcgtttgc
     5281 cgaacgaatg aaatattcgg caatgatgct gttttcgggc atatggtttt tattggttta
     5341 tgtgccgggc gcgcattggg tgtggggcgg cggctttatg agcaagggcg gcgtattgga
     5401 ttatgccggc ggtacggtgg tgcacatcaa tgccggtatc gcgggactcg tcgccgcctt
     5461 ggttttaggc aggcgcatag gctacgggcg cgaggcgatg cctccgcaca atatggcgat
     5521 gacgctgatt ggcgcggcaa tgttgtggtt cggctggttc ggctttaacg ctggttcggc
     5581 gcttgcggca gacgcggcgg cggggatggc gatggcggta acgcaggtgt cggccgtatt
     5641 cggcgcggca ggctggcttg cctgcgagaa aatagcggga cacaaacctt ctgctttggg
     5701 gctggcttcc ggcgcagttt ccgggctggt cggcatcacc cctgccgccg gctttaccgg
     5761 tccgtcgggc gcggccgcca tcggtatatt gactgccgcc gcgtgctttg tgtccgtcac
     5821 cgtcgtcaaa cacaaattgc gttacgatga ttctttggac gctttcggca tacacggatt
     5881 cggcgggctg gtaggcggaa tattgaccgg catctttttc gacaaccgca ttttcggtgg
     5941 ggatgcggca gtttggcagc agttgtggat acaggtaaaa gacgggttca ttatggcggc
     6001 atacagcggg ctgatgagtt gggcgatttt gaaggctgtg gggaaaatct gcggcggtct
     6061 gcgcgtcggc aaggatgtcg aacgcgaagg tttggatctg aatatccacg gcgaacacgt
     6121 ggaataagga cggctatgcc gtctgaagcc tgaaaatcgg gtttcagacg gcatttttca
     6181 cgtttgccgc cgatggggat aaacatactg cctgcgacgc ttaacggctg tctttccact
     6241 gataatattt cgagccgagg aaagaaccga gtttgcgcaa aaacggttgc aggataatat
     6301 tcggctggcg caactgttca aacggtttca gacggcattc gtcccctaaa attgcttcgg
     6361 caaccgccag gcctgcaatg cccgttatcg ccatcccgtg cccggaataa ccttgcgtat
     6421 aaaaaacatt cgaggtcaaa cgtccgaaat gcgggacaag gttggcggta atatcgcact
     6481 ccccgcccca cgaatattcg attctaacat cggcaagctg cggaaaaact ttaagcatat
     6541 cttggcggac aagctcggtc atacgcgcag gattgtcgat aaattcgtta tccttaccgc
     6601 cgaaaagcag cctgccgtcc gcgctgaggc ggtaataatc caaaatatgg cggttgtcgc
     6661 atactgccat attgttgcgg ataagccctt ttgtgcgcgc gcccaagggt tcggtggcaa
     6721 taataaaggt gctgacggca atcgccttgc gttccaaagg ccggaatatc gggttcaaac
     6781 cgacataagt attgacggca tagaccacat ttttacactc gacgctgcct tcgggcgtgt
     6841 aaaccagcca accgttttga tacggttcga tgcgcgtcat cggggattgc tcgaaaatct
     6901 gcgcgccggc ttcggcagcg gcgctggcaa cacccaacgt gtaattgagc ggatgaagat
     6961 gcccggacaa gggatcgaac tgtgcgcctt ggtacatatc gctgtcaagc tgctgtttca
     7021 actcggcttt atcccaaagt tgataatgac tcgcaccgta atgccgttgg gcgtgttcat
     7081 gccactgctg caactcttcc caatgctgcg gacggacggc aaccgtggca taaccgcgct
     7141 gccaatcgca atcgatggca tgtttgcgga cgcgttcgtc caccagttcg accgcctgca
     7201 aagactgttg ccaaaaccat tgcgcctgct ccaagccgac ctgtttttca atttccccca
     7261 taccgcaggc gtagtcgctg ataacctgcc cgccactcct gccggacgcg ccgaagccga
     7321 tacgtgcggc ttccaaaacg acggcttcat gtccgtgttc cgccagcggc aatgcggtac
     7381 acaaaccgct caaaccgccg ccgataatgc aggtttcggc tttcagacgg cattggagtt
     7441 tcggataaac agtatgcgga ttaaccgaac taaaataata agaaggcaga tattcttgaa
     7501 aatcagggcg aatcattgtg tttgctttat cgggtatatt ttcggacgga atgatacagg
     7561 ctgtcgggcc atatcgtcca aacagaaaat cggttgaaaa aaaacaggct gacccagtca
     7621 tgcggtcagc ctgccttatt aattaatttg ctttctcggc agccaatttt tcttggcggt
     7681 aggcttctgc cgcttctcgg ttgcgcttgg ttgcctgcct catcatccaa taattgacga
     7741 tgatgaccaa tgttccgatg atgccgatca ggatggttgc caagacattc atctgaggat
     7801 cgagacccaa cttgatcttg gagaaaataa cctgcggcaa tgtggatgaa ccggggccgg
     7861 agaggaatga ggtaatcacc aaatcatcca aagacaaggt aatgccgagt aggaagcctg
     7921 aagcaatggc aggggcaatc aaaggcaaag tgatgacaaa aaagattttc agcggacgcg
     7981 cgcccaagtc cattgcggct tcttcgagcg actggtcaag ctcgaccagc cgcgaacgga
     8041 tgacaacggt aatgtatgcc atgcacagcg tcgtatgtcc gaggaagatg gtgaaaaagc
     8101 cgcgatcgaa gtagagatgt tgcaaccatt cgctgccctg caaaaatatc tgcacctgaa
     8161 taatcagcag cagcatagac agaccggtaa tcacgtcggg catgaccatg ggcgcggaaa
     8221 tcataccggc aaacagcgtg ctgccgcgaa agcgtttgat acgcgccatc gcatagcccg
     8281 ccagcgtgcc caaaacgacg gcggcaagcg aagacacaac ggcaatccgc agcgacagcc
     8341 aagcggcttc caaaatggtg tcgttttcca gcaatgcgcc gtaccacttg gtcgaaaagc
     8401 cgccccaaac ggtaacaagt ttggattcgt tgaacgaata gatgaccaaa acaaccagcg
     8461 ggatatacag aaacgccagc gatagtgcca acatcagttt caagaaccaa gataatttgg
     8521 atttctgcat tatttggctc cttcttccaa ttcgcggttt tcatagtgct ggaacagggc
     8581 aatcggtaca accagtagcg cgaccatcac gacggcgacg gcagaagcca acggccagtt
     8641 gttttgatca aagaatgcct gccacaatac tttaccgatc atcaggtttt ccgaaccgcc
     8701 gaccagctcg ggaatgacga actcaccgac agcggggacg aaaaccagca tcgaacctgc
     8761 aataatgccg gttttcgaca aaggcagggt aatcgtcaag aacgatttga ccggtcccgc
     8821 gcccaagtcg gaagccgctt cgagcaggcg gctgtccagt ttcaccagct gcgtgtaaag
     8881 cggcagaatc ataaacggca gataggcata aaccattacc aaatttaacg aaaacgcatt
     8941 atagaacaaa tctaatggtt ctctgataat acccatctta atcaataagt tgtttatgat
     9001 gccgttatgc ccgagcaaac ccatccacgc atagacacgc agcaggaacg atgtccaaaa
     9061 aggtaacatg atggcaagca gcaaaccatt gcggacggaa ggattggcac gggaaatcgc
     9121 ataggcggtc gggtaaccga ccaacaggca aatcaccgtc gtcgtcagcg cagtcttaat
     9181 tgaagaccaa taagtcatca gatagatatt actgttttca ctgtcgccga aggggttaag
     9241 ggtatgccaa aaattttgga aaatatcggc atagttttga tagctgacgg cgatgttcag
     9301 gcgacccaag tcctcatcta tcgtcgttaa aggagtaaac ggtggaatgg cgatttcttg
     9361 ttcggcagag ctgattttca gcacgatggc gaacggaatc agaaacagca ccaaaagcca
     9421 aatatacggt acggcaatca ccgcacgctg ccccggacgg cggaacagtt tgtttttcag
     9481 tttattaagg ttcatctcac tccccttaaa tcaacggaac aacggagtcg gttggttttc
     9541 cggccagctg atatagacgg tttcatccca agtcggcggc gtgatgttac gcacatacca
     9601 gtaaggtgca ggaacttggc ttttgacgac gcgcccgttg gcgagtttga tatggtatat
     9661 ggcgaagctg cccaaatagg caatttcttt taccgtgcct ttcgcccagt tgtagtcgcc
     9721 caaatattcg ggtttttctt tatataaatc aatatcctct ggtcgaatac taacccaaag
     9781 atcctgctcg ctcgggccac ccaaaccatg gtcgatacgg acgtggtttt ccaaaccttc
     9841 gcattcgata acggcatagt cggcatgatt ttcaatcacc acaccgtcaa agatgttggt
     9901 ttcgccgata aactcggcag tgaagcggct gttgggatag tcgtacacgt cgctgggcgt
     9961 gccgacctgc tgcaactgac cgtcggacat aatggcgatg cgggtcgcca tcgtcatcgc
    10021 ctcttcttgg tcgtgcgtaa ccataatgca ggttacgccg acttgttcca gcgtattgac
    10081 caactcgagc tgggtttgct ggcgcaattt cttatccaac gcccccaaag gttcgtcaag
    10141 caacaggatt ttcggacgtt ttgccaaact gcgcgccaag gcgatacgtt gctgctgacc
    10201 accggacaat tggtgcggtt tgcgtttggc aaatttggtc atctgaacca gacgcagcat
    10261 ttcttcgacg cgcgcatcga tttcgccttt aggcattttg tcctgtttca gaccgaaggc
    10321 aatgttttgc tccaccgtca tatgcggaaa aagcgcgtaa ctttggaaca tcatattgat
    10381 ggggcggtca tagggtgcaa gtttggtaat atcttgacca tcaaggatga tttttccctg
    10441 attgggactt tccatacccg ccagcatacg cagcagcgta gattttccgc tgccggaact
    10501 gcccaaaagg gcgaagattt cgttttgata aatgttcaag tcgatgttat cgacagcgta
    10561 attgtcacca aactttttca ccaaaccttg gatttgcaga taaggtttgg ctgaagacgc
    10621 tgtggttgcg gtcataatgg caatactcca ataaaaagac gagtaccggc aaaacggatt
    10681 ttcgaatggg tgataaaaag ctgtttgatt gctggcggga gtcgcaacgt ctgatgccgt
    10741 ctgaaactct tgtgaagcgc acgggcagca tgaaataaaa aggggttcta aagaaccccg
    10801 cattatatta gtttataagg tttttagggt aataaatatg ggtttgagct tgtcggaaag
    10861 caacagaaaa gaaaacaccg cccatttttc tgggcggtgt cggaaagcgc aattatttac
    10921 gcagacccaa gcgggtaatc aacgcgcgat acgtatcggg ctgggtacgg cgcaaatagg
    10981 ccaacaggcg gcggcgttgg ctgaccattt tcaacaggcc gcgacggctg tggtggtctt
    11041 tggggttggc tttgaagtgg ggggtcagat cgttgatgcg gaaagtcaac agggcaactt
    11101 gtacttcaga agagccggtg tcgccttctt tacgttggaa atctttaacg atttgtgctt
    11161 tttgttctac ggtcagtgcc ataatggaaa aactccgaaa aataaaaaga accccaaatg
    11221 ggattcggac aagtttgcca agcctcgaca acggcaaact ccctattcct gaaacaggaa
    11281 cagcgcatta tcacacaatt tttgctgttc tgcacgatat tttgcgatgt cggcgaaatg
    11341 ccgtctgaaa acggagacat cttccggttt cagacggcat ttgacatcag accacaattt
    11401 cttccttctg tttcgcttcc ttggcgatat tgtcgcggct caaaccgaac atcagcaaga
    11461 gcgggctggc aaccaatacg gaagagtaga tgccgaacac gataccgatg gtcagtgcca
    11521 tagaaaagcc gtgcaaggct gcaccgccga acaccagcat agaaacaacc atcgcctcgg
    11581 tcgaaccgtg ggtaatgatg gtgcggctca tcgttgcggt aatcgcgttg tcaatgactt
    11641 ccggcacggt atgtccgcgc atcgccggct tgcggaaatt ttcacggata cggtcgaaga
    11701 cgacgacgga ttcgttcaca gaatagccca atacggcaag gatacctgcc aagacggtca
    11761 gcgaaaattc ccattggaag aaggcaaagc agccgagaat aatcacgatg tcgtgcatat
    11821 tggcgataat ggcagatacg gcaaaacgcc attcgaaacg catcgacagg taaataatga
    11881 tgccgataac gacaaaacct aaagccatca atccattatt taccaattcc tcaccgactt
    11941 gcgggccgat aaattcgact tggcgcaaag taacgtcggg actgtctttt ttcagcaaat
    12001 ccataacctg attggacaac tgtgcggaag taacaccttc tttgttcggc aggcggatca
    12061 tgatgtgttt gttcgtaccc aatgcctgaa cctgtacatc acccattttc agcgtatcga
    12121 ggcgttcgcg catcttattg acatccgcac cctgctgata ttggacttcc attaccgtac
    12181 cgccggtaaa ttcgacagag aaattcaggc ctctggcaac caaaaagaat acggcggcaa
    12241 taaaggtaac caacgaaatg aaggttgtca gtttgccgta gctcataaac ggaatatcgc
    12301 gtttgatttt aaagagttcc atagcttact ccttacctgc cgcagtttcg gcttcaggtt
    12361 tccataccgc accgatggaa atattctgca atttgcggcg gcggccgtac cacagattga
    12421 ccaacgcacg gaatacgacg acggacgaat acatcgaagt cagaataccc aagcagtgta
    12481 cgacggcaaa acctcttacc ggtccggaac cgaataccaa aagcgcgata ccggcaatca
    12541 gcgaagtcag gttcgaatca acaatggtcg cccatgcgtg ttggaaaccg agattgattg
    12601 cctgctgcgg cggcacgccg gcacgcaatt cctctcggat acgttcgtta atcaagacgt
    12661 tggagtcgat tgccataccc aaagtcaacg ccagcgcggc catacccggt aacgtcaacg
    12721 ttgcctgcat ggcagacaaa ataccgatta ggaacagtat gttggcactc aatgcaatag
    12781 tagaaaagaa acccatcagg cggtaataaa ccaccatgaa tgcagcaacg atggcaaaac
    12841 cccataaagt cgaatggaag cctttttcga tgttctcctt acccaaagac ggaccgatgg
    12901 tacgttcttc gacaatctgc atcggtgcgg caagagaacc ggcacgcaac agcaaagacg
    12961 tatcgttggc ttcggctgtc gtcatgcttc cggaaatttc cacgcgtccg ccggtaatgg
    13021 cagtacggat aaccggtgcg gttacaacct cggattttcc ttggtcgatc aaaaccatcg
    13081 ccatgcgttt gccgacattt gcggcagtca gttcgccgaa aatgctgccg cccgcgctgt
    13141 ccaagctcag actgacggca ggtgcgccca tttggtcgaa actcggttgc gcatcgttga
    13201 tgttgtcgcc ggtcagctcg acctgtttgc tgatcagcag agtttcggga tgctccccgc
    13261 cgcttgaaag cagctcataa ccgctcggca cgttgccttc caatgcctcg cgcaacttgg
    13321 caggatcgtc ctccaccatg cggacttcca aagtcgcggt acggccgatg atgtcttttg
    13381 ccttggcagt atcttgcacg ccgggaagct gtacgacaat gcggtccaaa cctgactgtt
    13441 ggataatcgg ctctgccaca cccaattcgt ttacacggtt atgcagtgta gtgatgtttt
    13501 gcttcaccgc atctgatcgt actttattca ccgcctcttc cgaaagcgcc aatacaatat
    13561 tgctgccttc tgaatttaac gttgcctcag gaaacagctt gagtaactga ggcagtgcct
    13621 tttgcacatc gccggcatcc tgcaaaggga cggtcaggct gttttcagcc tgacgcaccg
    13681 tgccgctgcg gattttttca cggcgcagtt cgcggcggat gtcgcccgaa taacgctcaa
    13741 acgttttctg catcgcggct ttcatatcga cctgcatggt gaaatgcacg ccgccgcgca
    13801 ggtccaaacc caaaaacatc ggattggctt tgattttcgc catccattcg gggctgtccg
    13861 ccaacaggtt gagcgcggta atataccctt cgcccaaagt gttttcgata acgtcgcgcg
    13921 ctttaagctg cgtttctgtg tctttgaaac gcactttcag tgaattatcc acaacaaaca
    13981 tcccgtcggt ctgaatgccc gcgtttttca gcgcagcatc cactttggat tgagtctgtt
    14041 cgttgatgat gatggcttgt cggttggtcg atacctgcac ggcgggcgtt tcgccgaata
    14101 ggttgggcag cgaatacact gcggcaaccg caatcgtgaa cacaatcagc agatatttcc
    14161 ataaaggata acggttcatc attgttcctt aatggttgga accccaccct ttcggtggtg
    14221 tcggaatcgg gctatttcag aagaggcaaa aacccctccc tgccaggcaa gaccgaaagg
    14281 cagcatcctg aatatgccgc cctgcgtgtc ggaacatggt caagccttcg gttggaattc
    14341 aaaacaaagt gccgcattcg ggctttcccg atgcggcttg tcggcacaaa tcaatcgact
    14401 tttgcggcaa tcgcattgcg ttccacttcg acctcgattt ttgtaccctg tccgatatcc
    14461 acggtaaaaa actgttcgcc gactctggtt accttaccct tgaaacctgc cgccaagacc
    14521 actttgtcgc cggctttcaa ggcggcaagc attgcctgat gcgctttgaa tttcttttgt
    14581 tgggggcgca tgatcaggaa gtagaacacc accataatca acaccaaagg agcaaattgt
    14641 gcaacagctt gattcataat ttatccgttc tttctaaata tgattgaaaa tcgagcgggg
    14701 tatataatag cataagaccg taaacaatat atcgggtttg ccgcccatac ccaaccgtat
    14761 gccgtctgca ccccctttcc cgaacccatg ccgctgaccc gcctgttcct gaaactctac
    14821 gccctgttgc gccttttttt gggcaaaaac gcccagaccg cctggatttt ccaccccgcc
    14881 tgcaccgggc acgaccccgg ggcaaaccat cccgattcgc ccgaccgcat cctctgcatc
    14941 gagcaggcat tgcgccgcgc cggtatttgg cagcacctcc aaaccgtaga ggcggaagaa
    15001 atcagcgata cgcgccttgc acttgtccgc tcgagcaaat atctgaaccg tttggaatct
    15061 tgcctacccg aagacggcaa gatttgccgt ttggacgacg acaccgttat cagcaaaaac
    15121 tccctgtccg ccgcccgttt ttccgccggt tcggcagttc aggcagtcga tatggtcatg
    15181 aaacgcaaag cgtggcacgc cttttgcgcc gcccgcccgc cggggcatca cgcaaaaagc
    15241 ggcaaagccg gcggattctg cctgctgaac aacgttgccg ccggcgtcat gcatgccatt
    15301 gccgaatacc gcctgaaacg cattgccgtc atcgatttcg atgtccacta cggcgacggt
    15361 acggcagaaa tattcaaaga cgatccgcgc atcctgtttt tcaacctgtt tgaaaccgac
    15421 cttttcccct tccccgaaaa caacggtatg cccgacggcg gcaatatggt gcacctgccc
    15481 ttgccgtcgg gaacgggcag ccgcacattc cgcgaagccg tccgcagaca gtggctgccc
    15541 cggcttgccg cattcaaacc cgaactggtg ctgctgtcgg caggattcga cgcacaccgt
    15601 ctggacgaat cgggcaggct caacctgcac gaggcggatt ttgcctggct gacacacaaa
    15661 atcattcaga cggcatccag ctgccccggc aaaatcgtat ccgtactcga aggcggctac
    15721 acccttgaac ctctggcaca atctgctgcc gagcatatcc gcgtactggc ggggctgggc
    15781 aaatccgatg ccgcaaccgc ctatcagaaa acactgaacc caacaaaaaa acggtttgcc
    15841 aaaccgaaaa ccggacaggt gcgccaacct acccaatccg accgttacga tataaatgcc
    15901 gtctgaagcc gattcggttt tcagacggca tcattatttg acctgacgca aacagaaagc
    15961 cttccgcgtg cttaaacaca tctcttgaga cctttgcaaa aatccccaaa atcccctaaa
    16021 ttcccaccaa ggcatttagg ggatttccca tgaacacctt cttccggcaa accgcccaag
    16081 ccatgactgc caaacacatc ggccgcttcc cgctatcgga gttggaccag gtgattgatt
    16141 ggcagccgat cgaacaatac ctga
//
LOCUS       DBJGGI010000050        15107 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.050, whole genome shotgun sequence.
ACCESSION   DBJGGI010000050 DBJGGI010000000
VERSION     DBJGGI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15107
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.050"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            322..1197
                     /gene="prpB"
                     /locus_tag="ACSIGJ_001519"
     CDS             322..1197
                     /gene="prpB"
                     /locus_tag="ACSIGJ_001519"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HHQ1330356.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVVGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLMVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLKMYRQFADAVKVPVLANITEFGATPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFDQKLDKLFQK"
     gene            1282..2430
                     /gene="prpC"
                     /locus_tag="ACSIGJ_001520"
     CDS             1282..2430
                     /gene="prpC"
                     /locus_tag="ACSIGJ_001520"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate synthase/citrate
                     synthase"
                     /protein_id="HHQ1330357.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSHRGYD
                     ILNLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLETLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPDSEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETVGGHFLHLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSC
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEADIRERIGRKEIVIGFGHPVYTISDP
                     RNVVIKEVARGLSREAGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVPTA
                     MFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            2582..3370
                     /locus_tag="ACSIGJ_001521"
     CDS             2582..3370
                     /locus_tag="ACSIGJ_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HHQ1330358.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKSLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIVATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIVSMF"
     gene            3447..6052
                     /gene="acnD"
                     /locus_tag="ACSIGJ_001522"
                     /pseudo
     CDS             3447..6052
                     /gene="acnD"
                     /locus_tag="ACSIGJ_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
     gene            6602..7764
                     /gene="prpF"
                     /locus_tag="ACSIGJ_001523"
                     /pseudo
     CDS             6602..7764
                     /gene="prpF"
                     /locus_tag="ACSIGJ_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
     gene            8075..9274
                     /locus_tag="ACSIGJ_001524"
     CDS             8075..9274
                     /locus_tag="ACSIGJ_001524"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HHQ1330359.1"
                     /translation="MSDQLILVLNCGSSSFKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFSKDGNKRQVPLSGRNCHAGAVGMLLNELEKHELHDRIQAVGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTGMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAVKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRSLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGIDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELVGIL"
     gene            9632..10252
                     /locus_tag="ACSIGJ_001525"
     CDS             9632..10252
                     /locus_tag="ACSIGJ_001525"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HHQ1330360.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKEVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMADGTELVRQGCGDTAVFGL"
     gene            complement(10414..10857)
                     /locus_tag="ACSIGJ_001526"
     CDS             complement(10414..10857)
                     /locus_tag="ACSIGJ_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HHQ1330361.1"
                     /translation="MYLTQHTDYGLRVLIYTAVNDDALVNIATIASTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLASPPERINIGAVVRHLEPMQLVECMGPNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRILIAVQ"
     gene            11031..11399
                     /locus_tag="ACSIGJ_001527"
     CDS             11031..11399
                     /locus_tag="ACSIGJ_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HHQ1330362.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFA
                     ELETHFREEEAKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            11444..12598
                     /locus_tag="ACSIGJ_001528"
     CDS             11444..12598
                     /locus_tag="ACSIGJ_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HHQ1330363.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     VSASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKSVPFAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(12655..13527)
                     /locus_tag="ACSIGJ_001529"
     CDS             complement(12655..13527)
                     /locus_tag="ACSIGJ_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HHQ1330364.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVVIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHHCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALIENLWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            13612..14424
                     /locus_tag="ACSIGJ_001530"
     CDS             13612..14424
                     /locus_tag="ACSIGJ_001530"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HHQ1330365.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAEEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTIRAGGEVPHLSAEGVPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGVVTADIDANRLNSVRNRLPALKYRVLDAV"
     gene            complement(14869..15048)
                     /locus_tag="ACSIGJ_001531"
     CDS             complement(14869..15048)
                     /locus_tag="ACSIGJ_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330366.1"
                     /translation="MPDNTVISKPVIPDNSAILKPVIPDNTVISKFVIPAQAGIQTCP
                     HRNLSDKNSCPNPAF"
BASE COUNT         3409 a         4030 c         4235 g         3433 t
ORIGIN      
        1 atgtttgaca ttccagtctt gtgttttaga ttaccgaaaa ataaaactac ataatactac
       61 aaaggaacat tactatgaaa ccaattcaga tgtttttccc ttttctgaat aatccccttg
      121 ttttcttctt gtctgcggtt ttgccgcata attccgaacg gtctgctgtt tttctttgat
      181 tcgttttaaa tatcaataag ataatttttc ccatatattt ttaatgattg ggatgcccga
      241 cgcgtcggat ggctgtgttt tgccgtccga atgtgatgga agcctgtcca tactgaaaaa
      301 agtctataaa ggagaaatat gatgagtcaa cactctgccg gagcacgttt ccgccaagct
      361 gtgaaagaat cgaatccgct tgccgtcgtc ggttgcgtca atgcttactt tgcacgattg
      421 gccacccaaa gcggtttcaa agccatctat ctgtccggcg gcggcgtggc agcctgttct
      481 tgcggtatcc ctgatttggg cattaccaca atggaagatg tgctgatcga cgcacgacgc
      541 attacggaca acgtggatac gcctctgatg gtggacatcg atgtgggttg gggcggtgca
      601 ttcaatattg cccgtaccat tcgcaacttt gaacgcgccg gtgttgcagc ggttcacatc
      661 gaagatcagg tagcgcaaaa acgctgcggc caccgtccga acaaagccat cgtttccaaa
      721 gatgaaatgg tcgaccgcat caaagctgcc gtagatgcgc gcgttgatga gaacttcgtg
      781 attatggcgc gtaccgatgc gctggcggta gaaggtttgg atgccgctat cgaacgcgct
      841 caggcttgtg tcgaagccgg tgcggacatg attttccctg aagccatgac cgatttgaaa
      901 atgtaccgtc aatttgcaga tgcagtgaaa gtaccagtat tggccaacat taccgagttt
      961 ggtgctacac cgctttatac gcaaagcgag ttggctgaaa acggcgtgtc actggtgctg
     1021 tatccgttgt catcattccg cgcagcaagc aaagccgctt tgaatgttta cgaagcgatt
     1081 atgcgcgatg gcactcaggc ggcggtggtg gacagtatgc aaacccgtgc cgagctgtac
     1141 gagcatctga actatcatgc cttcgatcaa aaactggata aattgtttca aaaatgattt
     1201 accgctttca gacggccttt caacaaatcc gcatcgaccg tctgaaaaat aaaacccata
     1261 aaaatacaaa ggagaaatac catgactgaa actactcaaa ccccgacctt caaacctaaa
     1321 aaatccgttg cgctttcagg tgttgcggcc ggtaataccg ctttgtgtac cgttggccgt
     1381 accggtaacg atttgagcca tcgcggttac gacattctaa atttggcaca aaaatgcgag
     1441 tttgaagaag tcgcacactt gctgattcac ggccatctgc ccaacaaatt cgagctggcc
     1501 gcttataaaa ccaagctcaa atccatgcgc ggcctgccta tccgtgtgat taaagttttg
     1561 gaaaccctgc ctgcacatac ccatccgatg gacgtgatgc gtaccggcgt atccatgctg
     1621 ggctgcgttc atcccgaacg cgaaagccat cccgacagcg aagcgcgcga catcgccgac
     1681 aaactgatcg ccagcctcgg cagcatcctg ctctactggt atcaatattc gcacaacggc
     1741 aaacgtattg aagttgaaag cgacgaagag accgtcggcg gtcatttcct gcacctgttg
     1801 cacggcaaac gcccaagcga atcacacatc aaagccatgc atgtttcact gattctgtat
     1861 gccgaacacg agttcaacgc ctccactttt actgcccgcg tgattgccgg tacgggttcg
     1921 gatatgtatt cctgcatcac cggcgccatc ggcgcgctta aaggtccgaa acacggcggt
     1981 gcgaacgaag tcgcctacga catccaaaaa cgctaccgca acgccgacga agccgacatc
     2041 cgcgaacgca tcggccgcaa agaaatcgtg atcggtttcg gccatccggt ttacaccatt
     2101 tccgacccgc gcaacgtcgt gattaaagaa gtggcgcgcg gtttgagccg ggaagcgggc
     2161 gacatgcgcc tcttcgacat cgccgaacgc ttggagagcg tgatgtggga agagaaaaaa
     2221 atgttcccga atctggactg gttctctgcc gtttcctacc aaaaattggg cgtaccgacc
     2281 gctatgttca caccgctgtt cgtgatttcc cgtacaaccg gttggagcgc acacgtcctc
     2341 gaacagcgca aagacggcaa aatcatccgt ccgagcgcaa actacaccgg ccctgaagat
     2401 ttggcgtttg tggagattga agaacgataa ttgaagaatg caatagcagt ttgttcttta
     2461 tttcggtatg caaagctgag gatttcagac gaccttgcct tattggaaag gttgtctgaa
     2521 ataagtttaa tctaatagga gaagataatc ctgtattggc gcaagtaaca ggataagaaa
     2581 catggaagat ttatacataa tactcgcttt gggtttggtt gcgatgatcg ccggatttat
     2641 cgatgcgatt gcgggcgggg gtggtttgat tacgctgcct gcactcttgt tggcaggtat
     2701 tcctcccgtg tcggcaattg ccaccaacaa gctgcaagca gccgctgcta cgttttcggc
     2761 tacggtttct tttgcacgca aaggtttgat tgattggaag aaaagtctcc cgattgccgc
     2821 agcatcgttt gcaggcggcg tggtcggtgc attatcggtc agcttggttt ccaaagatat
     2881 tttgctggcg gtcgtgccgg ttttgttgat atttgtcgcg ctgtattttg tgttttcgcc
     2941 caagctcgac ggcagtaagg aaggcaaagc cagaatgtct ttttttctat tcgggctgac
     3001 ggttgcaccg cttttgggtt tttacgacgg tgtgttcgga ccgggtgtcg gctcgttttt
     3061 tctgattgcc tttattgttt tgctcggctg caagctgttg aacgcgatgt cttacaccaa
     3121 attggcgaac gttgcttgca atcttggttc gctatcggta ttcctgctgc acggttcgat
     3181 tattttcccg attgtggcaa cgatggcggt cggtgcgttt gtcggtgcga atttaggtgc
     3241 gagatttgcc gtccgcttcg gttcgaagct gattaagccg ctgctgattg tcatcagcat
     3301 ttcgatggct gtgaaattgt tgatagacga gagaaatccg ctgtatcaga tgattgtttc
     3361 gatgttttaa accctttcag acgactcctt caaaacatcg gctgaaaccc aaaccacaag
     3421 aaaaatagat ccacaggaga accgacatga ctgccaacca aagttaccgc aaaccgctgc
     3481 ccggtacgga tttggaatac tacgacgcgc gtgcggcgtg tgaggacatc aaacccggct
     3541 cttacgacaa gctgccgtac acgagccgca ttttgtctga gaacttggtc aaccgcgcgg
     3601 acaaagtcga tttgccgatg ctgcaaagct ggctgggcca gttgatagaa gggaagcagg
     3661 aaatcgattt tccgtggtat cccgcgcggg tggtgtgcca cgatattctg gggcagaccg
     3721 cgctggtgga tttggcaggc ttgcgcgatg cgattgccga aaaaggcggc gatcctgcca
     3781 aagtgaatcc ggtggtgcag acgcagctta tcgtcgacca ctctttggcg gtggagtgcg
     3841 gcggttacga tcctgatgcc ttccgcaaaa accgcgaaat cgaagacaga cgtaacgaag
     3901 accgtttcca cttcatcaac tggacaaaaa ccgcatttga aaatgtggac gtgattccgg
     3961 cgggcaacgg catcatgcac caaatcaatc tggaaaaaat gtcgcccgtc gtccaagtca
     4021 aaaacggcgt ggctttcccc gatacctgcg tcggcacgga ctcgcatacg ccgcacgttg
     4081 atgcgctggg cgtgatttcc gtggcgtggg cggtttggaa gcggaaaccg tgatgctggg
     4141 acgcgcgtcc atgatgcgcc tgcccgatat tgtcggcgtg gagctgacgg gcaaacggca
     4201 ggcgggcatt actgccacag acatcgtgtt ggcactcacc gaattcttgc gtaaagagcg
     4261 cgtggtcggg gcgtttgtcg aatttttcgg cgagggcgcg agaagcctgt ctatcggcga
     4321 ccgcgcgacc atttccaaca tgacgccgga gttcggcgcg actgccgcca tgttcgccat
     4381 cgacgcgcaa actattgatt atttgaaact gaccggacgt gacgacgcgc aggtgaaatt
     4441 ggtggaaacc tacgccaaaa ccgcaggctt atgggcaggt ggcttgaaaa ccgccgttta
     4501 tccgcgcgtt ttgaaatttg atttgagcag cgtaacgcgc aatatggcag gcccgagcaa
     4561 cccgcacgcg cgttttgcca ccgccgattt ggcggcgaaa gggctggcga agccttacga
     4621 agagccttca gacggccaaa tgcctgacgg tgcagtgatt attgccgcga ttacttcgtg
     4681 taccaatact tccaacccgc gcaacgttgt cgccgccgca ctgttggcac gcaatgccaa
     4741 ccgcctcggc ttgaaacgca aaccttgggt gaaatcttcg tttgccccgg gttcaaaagt
     4801 agccggaatc tatttgaaag aagcaggctt gttgcccgaa atggaaaaac tcggcttcgg
     4861 tatcgtcgcc ttcgcatgta ccacctgtaa cggcatgagc ggcgcgctcg acccgaaaat
     4921 ccaacaagaa atcatcgacc gcgatttgta cgccaccgcc gtattgtcag gcaaccgcaa
     4981 cttcgacggc cgtatccatc cgtatgcgaa acaggctttc ctcgcttcgc ctcctttggt
     5041 cgttgcctac gcattggcag gtagcatccg tttcgatatt gaaaacgacg tactcggcgt
     5101 tgcagacggc cgcgaaatcc gcctgaaaga tatctggccg acagacgaag aaatcgatgc
     5161 catggttgcc gaatatgtga aaccgcaaca attccgcgac atttatatcc cgatgtccga
     5221 caccggcaca gcgcaaaaag caccaagccc gctgtacgac tggcgaccga tgtccaccta
     5281 catccgccgt ccgccctatt gggaaggcgc actggcaggg gaacgtacat taagaggtat
     5341 gcgtccgccg gcgattttgc ccgacaacat caccaccgac cacatctcgc catccaatgc
     5401 gattttggcc ggcagtgccg caggcgaata tttggcgaaa atgggtttgc ctgaagaaga
     5461 cttcaactct tacgcaaccc accgcggcga ccacttgacc gcccaacgcg caaccttcgc
     5521 caatccgaaa ctgtttaacg aaatggtgag aaacgaagac ggcagcgtac gccaaggttc
     5581 gttggcacgc gttgaaccag aagaccaaac catgcgcatg tgggaagcca tcgaaaccta
     5641 tatgaaccgc aaacagccgc ttatcatcat tgccggtgcg gactatggtc aaggctcaag
     5701 ccgcgaccgg gcggcgaagg gcgtgcggct ggcgggtgtg gaagccatcg ccgccgaagg
     5761 tttcgagcgc atccaccgca ccaacctcat cggcatgggc gtcttgccgc tgcaattcaa
     5821 acccggcacc aaccgccata ccctgcaact ggacggtacg gaaacctacg acgttgtcgg
     5881 cgaacgcaca ccgcgctgcg gcctgaccct cgtgattcac cgtaaaaacg gagaaaccgt
     5941 cgaagttccg gttacctgcc gccccgatac tgcagaagaa gtattggtat atgaagccgg
     6001 cggcgtgttg caacggtttg cacaggactt tttggaaggg aacgcggctt agagttcgtc
     6061 tgaaaagcaa gacgtagcgt gggtcgggtt ctcattcacg taattcccga tatggcaggc
     6121 atttactgta aatcgtcatt cccgcgcagg cgggaatcca gaaagtggaa ttgaggaaac
     6181 ctttttatcc gatgagtttc tgtgcggata aatctggatt tccgcctacg cgggaatgac
     6241 ggggtttaat aatctaccgt atcacaacac agcagccgta gattggggcg aaccccgaca
     6301 gtttgcggaa tcaaacggct ttggtcggag tggtagccta atgtacttct ggaaagtggg
     6361 tgtagcgtgg gctttgccca cgaaataaag gctgaattga catggtatag cggattaaca
     6421 aagatcagga caaggcggcg ggccgcaggc agtacaaatg gtacggaacc ggttcgcccg
     6481 gtgcttgggc gccttaggga atcgttccct ttgagccggg gcggggcaac ccgtaccggt
     6541 ttaaattaat ccactataaa aatcgtgggc agagcccacg ctacataagg agaaccctaa
     6601 aatgccgcaa atcaaaatcc ccgccgttta ctaccgcggc ggtacatcta aaggcatttt
     6661 cttcaaacgc accgatctgc cggcagctgc gcaggaagca ggcggggcac gcgacaaaat
     6721 cctcttgcgc gtactcggca gccccgaccc ttacggcaaa cagattgacg gtttgggcaa
     6781 cgccagttcg tccaccagca aagctgtgat ttcggacaaa tccgaacgcg ccgatcacga
     6841 tgtcgattac ttgttcggac aagtttccat cgacaaacca tttgtcgatt ggagcggcaa
     6901 ctgcggcaac cttaccgccg ccgtgggcac attcgccatc gaacagggct tggtcgataa
     6961 atccaaaatc ccttcagacg gcatctgcac ggtcaaaatc tggcagaaaa acatcggcaa
     7021 aaccattatt gcccatgtgc cgatgcaaaa cggagcagtg ttggaaacag gcgatttcga
     7081 gcttgacggc gtaattttcc ggcagccgaa gtacaaatcg aatttttaga tccagccgac
     7141 ggcgaaggca gtatgttccc aaccggcaat ttggtcgacg aaattgatgt gccgaatata
     7201 ggtcgtctga aagccacgct catcaacgcg ggcattccga ccgttttcct gaacgccgcc
     7261 gacttgggct acacgggcaa agagttgcag gacgacatca acaacgatgc cgccgcgctg
     7321 gaaaaatttg aaaccatccg cgcatatggc gcgctgaaaa tgggtttgat cagcgacgta
     7381 tccgaagccg ccgcccgcgc gcacacgccg aaacccgcct tcgtcgcgcc cgccgccgat
     7441 tacaccgcct ccagcggcaa aaccgtaaac gccgccgaca tcgatttgcc ggtacgcgcc
     7501 ctgagcatgg gcaaactgca ccacgctatg atgggcatcg cctcggtcgc catcgccgcc
     7561 gccgtgctcg gtacgctggt caaccttgcc gcaggcggcg gaacgcgtaa agaagtgcgc
     7621 ttcgggcatc cgtcaggtac gctgcgtgtc ggtgctgccg ccgaatgtca ggacggacaa
     7681 tggacggccg ccaaagcggt catgagccgc agcgcacgcg tgattatgga aggttgggtg
     7741 cgcgttcccg atgattgttt ttgaaatcat gtaggacgca cattgctgtc cgctgtttca
     7801 gacggcattt gattaaggat taccataaag ccgaataacc ttaccgccgt cattcccacg
     7861 caggcgggaa tccgcagccg atattctgtt tcctgatttt tatcagagcc tgcgtgtgag
     7921 caaatatgga ttcccgcctg cgtgggaatg acgctaggaa atacggcata cgctttgccc
     7981 aaagaggccg tctgaaacac actccgccca acgcccatcc tttttcagac ggccccgcac
     8041 caaaaaacaa ccacaaacta caaggagaaa catcatgtcc gaccaactca ttcttgttct
     8101 gaactgcggc agttcatcgt tcaaaggtgc cgttatcgac cgcaaaagcg gcagcgtcgt
     8161 cctaagctgc ctcggcgaac gcctgaccac gcccgaagcc gtcattacgt tcagcaaaga
     8221 cggcaacaaa cgccaagttc ccctgagcgg ccggaactgc cacgccggcg cggtgggtat
     8281 gctgttgaac gaactggaaa aacacgaact gcacgaccgc attcaagccg tcggccaccg
     8341 catcgcccac ggcggcgaaa aatacagcga gtctgttttg atcgaccagg ccgtaatgga
     8401 cgaactcaat gcctgcattc cgcttgcgcc gctgcacaac cccgccaaca tcagcggcat
     8461 cctcgccgca caggaacatt tccccggtct gcccaatgtc ggcgtgatgg atacttcgtt
     8521 ccaccaaacc atgccggagc gtgcctacac ttatgccgtg ccgcgcgagt tgcgtaaaaa
     8581 atacgctttc cgccgctacg gtttccacgg caccggtatg cgttacgtcg cccctgaagc
     8641 cgcacgcatc ttgggcaaac ctctggaaga catccgcatg attattgccc acttaggcaa
     8701 cggcgcatcc attaccgccg tcaaaaacgg caaatccgtc gataccagta tgggtttcac
     8761 gccgatcgaa ggtttggtaa tgggtacacg ttgcggcgac atcgatccgg gcgtatacag
     8821 ctatctgact tcccacgccg ggatggatgt tgcccaagtt gatgaaatgc tgaataaaaa
     8881 atcaggcttg ctcggtattt ccgaactctc caacgactgc cgttctttgg aaatcgctgc
     8941 ggatgaaggc catgaaggcg cacgtttggc tctggaagta atgacttacc gccttgccaa
     9001 atacattgct tcgatggctg tggcttgtgg cggcattgat gcactggtat ttaccggcgg
     9061 tatcggcgaa aactcgcgta atatccgagc taaaaccgtt tcctatcttg atttcttggg
     9121 tctgcacatc gacaccaaag ccaatatgga aaagcgctac ggcaattcgg gtattatcag
     9181 cccgaccgat tcttctccgg ctgttttggt tgtcccgacc aatgaagaac tgatgattgc
     9241 ctgcgacact gccgaacttg tcggcatctt gtagccaaaa aagggacgag tccgcacaat
     9301 gccgtctgaa accccaaacg cccgattagg ctgatgagga ttttagacgg cattgttcat
     9361 ttttttgtta tcttgcattt ttgtgcggac ggtggaattt catcctgtaa acataaatat
     9421 ttgtcggaaa acagaaaccc tccgccgcca tttctacgaa agcaggaaac cagcaacgca
     9481 aagcgacagg gatttgttgg aaatgaccga aaccgaacga accggattcc cgcctgcgcg
     9541 ggaatgacgg gattttctgt ttttgtggaa atgacgggat tttgaatttc gggcgtacaa
     9601 tacggaaaac atgacgataa ggaaaaaaac tatggcacag tttttcgcta ttcatcccga
     9661 caatccccaa gaacgcctca tcaagcaggc ggtcgaaatc gtcaataaag gcggcgtggt
     9721 ggtttatccg accgattcct gttatgcctt gggctgcaaa ctcggcgata aggcggcgat
     9781 ggaacgcata ctctccatcc gaaaaatcga tttgaaacac cacctgaccc tgatgtgcgc
     9841 cgatttgagc gagttgggca cgtacgccaa aatcgacaac gtacagtttc gtcagcttaa
     9901 agccgccacg cccgggcctt atacctttat cttgcaggcg acgaaggaag tgccggcgcg
     9961 caccctgcac ccgaaacgca aaaccatcgg gctgcgtatt cccgataatg ccattgcaca
    10021 agccctgctg ggggaattgg gcgagccgct tttaagctgc accctgatgc tgcccgaaga
    10081 cggcgaacca ttgaccgatc cttatgaaat ccgcgagcgt ttggaacacg ccgtcgattt
    10141 ggtgattgac ggcggctggt gcggaaccga gccgaccacc gtcgtcgata tggccgacgg
    10201 cacggaattg gtgcgccaag gttgtggcga tacggcggtg ttcggtttgt agggaaaccg
    10261 atgccgtctg aagcataggc tgttcagacg gcatcgcgcg ccttgccggc ggcagcccga
    10321 aatgccggcg cgtatcgcgc tcggtcggaa tatccgtttg aaacggcaaa caaaatgccg
    10381 tccgaacgcg ggttcggacg gcattttgat gcgttactgc accgcaatca gaattctcgg
    10441 ttcgtagagc aggtcgtagg tcggcttgtt gagcaggtct tgcagcgtga aaccgtccag
    10501 atacgtgaaa aacgacttca tcgcgccgcc gagtatgccc gtcagacggc aggacggcgt
    10561 aatcaggcat tcgttgttcg ggcccataca ttcgaccagc tgcatcggtt cgaggtggcg
    10621 gacgaccgcg ccgatattga tgcgttcggg cggcgaggcc agcctcagcc cgccgccttt
    10681 tccgcgtacg ctgtggagga agccgccttt gaccagcgcg gtaaccacct tcatcaaatg
    10741 gcttttggaa atgccgtagg tcgaggcgat ggtggcgatg ttgaccagcg cgtcgtcgtt
    10801 gacggcggtg tagatgagga cgcgcagccc gtagtccgta tgttgtgtca aatacatgat
    10861 tttctcggta tggattgtta ttcttatcgg tacggtttaa ggttcacgga caatacctta
    10921 atggttgaaa ccctgtccgt cggggcggta gaatgcagcc tgtctgcggc ggtatgccgt
    10981 ctgaaacatc cgcgctaccg tttgataatt tgttattgta actcaaaatc atgaaaccgt
    11041 tgaaacgaca tcccgccctt atcgggcttt cgcgtgacca ccaccattcg ctttccctgt
    11101 gcgtgcgtct gttgcggacg ccggaagaaa ggcatcggga cgaactcgaa ccgcattttg
    11161 ccgaattgga aacccatttt cgcgaagaag aagccaagtt tgccccaatt tggcagaatg
    11221 tcgcccccga attgaaacaa cgtttcgaga aagaccacgc ccgactgcgg cagatgatgg
    11281 caagccccga atacggtaac gcggcgtgga ataccgcttt tgccacaacc ctgcgcgacc
    11341 acgcgcgctt tgaagaacgc gagctgtttc ccgccgccga accgtttttg ccggcatgat
    11401 tccgttttgc ggcaaatata ttaatgataa acaaggaaca cacatgaaat ttaccaagca
    11461 ccccgtctgg gcaatggcgt tccgcccatt ttattcgctg gcggctctgt acggcgcatt
    11521 gtccgtattg ctgtggggtt tcggctacac gggaacgcac gagctgtccg gtttctattg
    11581 gcacgcgcat gagatgattt ggggttatgc cggactggtc gtcgtcgcct tcctgctgac
    11641 cgccgtcgcc acttggacgg ggcagccgcc cacgcggggc ggcgttctgg tcggcttgac
    11701 tatcttttgg ctggctgcgc ggattgccgc ctttatcccg ggttggggtg tgtcggcaag
    11761 cggcatactc ggtacgctgt ttttctggta cggcgcggtg tgcatggctt tgcccgttat
    11821 ccgttcgcag aatcaacgca actatgttgc cgtgttcgcg ctgttcgtct tgggcggcac
    11881 gcatgcggcg ttccacgtcc agctgcacaa cggcaaccta ggcggactct tgagcggatt
    11941 gcagtcgggc ttggtgatgg tgtcgggttt tatcggtctg attggtacgc ggattatttc
    12001 gttttttacg tccaaacggt tgaatgtgcc gcagattccc agtccgaaat gggtggcgca
    12061 ggcttcgctg tggctgccca tgctgaccgc catgctgatg gcgcacggcg tgatgccttg
    12121 gctgtcggcg gctttcgcgt ttgcggcggg cgtgattttt accgtgcagg tgtaccgctg
    12181 gtggtataag cctgtgttga aagagccgat gctgtggatt ctgtttgccg gctatctgtt
    12241 taccggattg gggctgattg cggtcggcgc gtcttatttc aaacccgctt tcctcaatct
    12301 gggtgtgcat ctgattgggg tcggcggtat cggcgtgctg actttgggca tgatggcgcg
    12361 taccgcgctc ggtcatacgg gcaatccgat ttatccgccg cccaaatccg ttccgtttgc
    12421 gttttggctg atgatggcgg caaccgccgt ccgtatggtt gccgtatttt cttccggcac
    12481 tgcctacacg cacagcatac gcacctcttc ggttttgttt gcactcgcgc ttttggtgta
    12541 tgcgtggaag tatattcctt ggctgattcg tccgcgttcg gacggcaggc ccggttgaga
    12601 caaaccgccg cagatttcgg gtctgggcgg tttgcttttc agacggcagg gcggtcagtt
    12661 gccgtccagc cagcggtcgc gtgtggtttt ggcttcttca aaatagcggt acagggcttc
    12721 gcggtcgtcg gtggtcagga tgtttgccaa aacgtccaac tgtttgccca agccttgaac
    12781 cagttgcagc aggctgtctt tgttggcaag gcagatgtcc gcccacacgg cgggatggcc
    12841 ggaggcgatg cgggtgaagt cccgaaagcc cgtggcggcg aatttcagat attcctgtcc
    12901 gtcggggtgg tcgagaatct ggtggacata ggcgaaggcg gtcaggtggg gcatatggga
    12961 gacggcggcg aaaaccgcgt cgtggcgttg cgcgtccatc gtataaattt ccgccccgac
    13021 cgcgtgccac aggttttcta tcaaggcaat gccgtctgaa tgttcgccgc cgtgtggcgt
    13081 gatgatgagt tttctgtggc ggaacagccc gaactgcgcg gcttgcgcac cgcttctgtc
    13141 cgaaccggca atcgggtggg cggcgatgca gtggtgcagg cggtcgggca gacagcggcg
    13201 gaaggcttcg atgaccgaag atttggtgct gccgacatcg gaaatccaag tgtgttccgg
    13261 caaaacgggg cgcagcgcgg tcaaaatggc aggaacggtg gcgacgggcg tggcaatcag
    13321 taccaagtcc gcaccgccga tgctgtctgc gtcgatgacg acggaagccc ggtcaatcac
    13381 gccgcgttcc aatgcacgtt cgaggttgtc gcggtcggtg tcgataccgg taacggtgcg
    13441 gacgagtccc tgccttttga ggtcgagaac gaacgaaccg ccgatcagcc ctacaccgat
    13501 gagggcaata tggttcaaaa tgggcatttg tgtaaacggt tttcgcaaag tgccgccatg
    13561 gtagcctatc ggcggaatat gccgcaaggt cggcaggaaa aaggagaaga aatggacaaa
    13621 atcagagttg ccgccgtgca gatggtgtcg ggcgtgtcgc cggaaaccaa cgtcgccgcc
    13681 atgaaacgcc tggtcgcacg ggcggaggag cagggtgcgg attgggtgct gctgcccgaa
    13741 tattgggtgc tgatgggcgc aaacgatacc gacaaactcg cgcttgccga gcctttgggc
    13801 ggcggacgct ttcagacggc attgagcgaa acggcgaaag aatgcggcgt ggtgctgttc
    13861 ggtggaaccg tgccgctgca aagccccgag gcgggcaagg tcatgaatac tttattggtg
    13921 tacgggcgcg acggcaaacg gacggggctg taccacaaaa tgcacctctt cggcttttcc
    13981 ggtttgggcg aacgctatgc cgaagccgat accatccgcg cgggcgggga agtgccgcac
    14041 ttgtcggcag agggcgtgcc ggtggcggca ggtatttgtt acgatgtccg ctttcccgaa
    14101 tttttccgcc gccagctgcc gtttgacgta ttgatgctgc ccgctgcgtt tacgcacacg
    14161 acgggcaagg cgcattggga gctgctgctg cgcgcgcgtg ccgtcgaaaa ccaatgttac
    14221 gtcgtggcgg cggcacaggg cggtttgcac gaaaacggac ggcgcacgtt cggacacagc
    14281 atgattgtcg atccgtgggg cgacgtgttg gacgtattgc ccgaaggcga aggcgttgtt
    14341 acggcagaca tcgatgccaa ccgcctgaac agcgtccgca accgcctgcc cgccttgaaa
    14401 taccgggttt tggatgccgt ctgaaggttc agacggcatc ggtgccgggg aatcagcggg
    14461 ctgtaggatg ggcttcagcc tgccatttcc atcaatccca catttctacc gttttcatcg
    14521 aatccatcga atccgcccct ttcgacgccc cagtcctacg caactgaacc gtcattccca
    14581 cggaagtggg aatctagaat ctcggggttt cagtcatttc cgatagattc ccgccgcgtc
    14641 ggaggtctgg attcccgcct gcgcgggaat gacgggtttt aagattacgg tgttgtcgga
    14701 acgaaactga atcgtcattc ccacggaagt gggaatctag aatctcgggg tttcagtcat
    14761 ttccaataga ttcccgccgc gtcggaggtc tggattcccg cctgcgcggg aatgacggat
    14821 ccatccatac ggaaacctgc accacgtcat tcccacggaa gtgggaatct agaacgcggg
    14881 gtttgggcaa ctgtttttat ccgataagtt tctgtgcgga caggtctgga ttcccgcctg
    14941 cgcgggaatg acgaatttcg agattacggt gttgtcggga atgacgggtt tcaagattgc
    15001 gctgttatcg ggaatgacgg gtttcgagat tacggtgttg tcgggaatga cggttcgggt
    15061 atttccttac gcctgtcctg cgcctgtaaa cggcaagtgc atcaaaa
//
LOCUS       DBJGGI010000051        14713 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.051, whole genome shotgun sequence.
ACCESSION   DBJGGI010000051 DBJGGI010000000
VERSION     DBJGGI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14713
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.051"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(612..728)
                     /locus_tag="ACSIGJ_001532"
                     /pseudo
     CDS             complement(612..728)
                     /locus_tag="ACSIGJ_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            925..2034
                     /gene="fic"
                     /locus_tag="ACSIGJ_001533"
     CDS             925..2034
                     /gene="fic"
                     /locus_tag="ACSIGJ_001533"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HHQ1330367.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLASRPLCTQTAIMVCNAIKHPYETAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLVIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRCLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     GIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSF"
     gene            complement(2762..3574)
                     /gene="murI"
                     /locus_tag="ACSIGJ_001534"
     CDS             complement(2762..3574)
                     /gene="murI"
                     /locus_tag="ACSIGJ_001534"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HHQ1330368.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLGHDVKAMVIACNTIAAVAGRKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLDTGNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
     gene            3762..4223
                     /gene="tsaE"
                     /locus_tag="ACSIGJ_001535"
     CDS             3762..4223
                     /gene="tsaE"
                     /locus_tag="ACSIGJ_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HHQ1330369.1"
                     /translation="MSDFSPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFALHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHGGGGRKCLLTAHTERGSESLPL"
     gene            4220..5470
                     /locus_tag="ACSIGJ_001536"
     CDS             4220..5470
                     /locus_tag="ACSIGJ_001536"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HHQ1330370.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFALDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPTHAQVFALPPVGGFKDRLVVDLYPHGMDADDPMMALLNGS
                     LNKTLRGSPEADLAQNTTPRPGRGKNGRRPVIMLDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDITQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            5552..6370
                     /locus_tag="ACSIGJ_001537"
     CDS             5552..6370
                     /locus_tag="ACSIGJ_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HHQ1330371.1"
                     /translation="MKGMDKLRYQRDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSPAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPKPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLRYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDVDIFRNFSI"
     gene            complement(6416..7558)
                     /locus_tag="ACSIGJ_001538"
     CDS             complement(6416..7558)
                     /locus_tag="ACSIGJ_001538"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HHQ1330372.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTEVRAFDGGVSCRGGL
                     EQAYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKIEAKR
                     ANVKSLQFAGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAAGRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQTRPVRAPIAGSDNGRRIVQTALDNAHNA
                     GADDIVSFSVADAQSVRPNGENGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLLPKRKIPLYNGNIDCRLFLIDMVQGSNR"
     gene            complement(7607..8029)
                     /locus_tag="ACSIGJ_001539"
     CDS             complement(7607..8029)
                     /locus_tag="ACSIGJ_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330373.1"
                     /translation="MNKNRKLLLAVLLLTAFAAFKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GNWQAARIRLPVCVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(8022..8831)
                     /locus_tag="ACSIGJ_001540"
     CDS             complement(8022..8831)
                     /locus_tag="ACSIGJ_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HHQ1330374.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLGSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFGEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWEGGPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLYGENSMGAYRVLP
                     LGSAGGSGANVLMEAAQWQDRSEHAGSVWMMVQTREQWRQAQEKGADVVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHAVVRDETIGKNHE"
     gene            complement(8913..9290)
                     /gene="acpS"
                     /locus_tag="ACSIGJ_001541"
     CDS             complement(8913..9290)
                     /gene="acpS"
                     /locus_tag="ACSIGJ_001541"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HHQ1330375.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFCNIGIGHDALGKPEFFYGPALSEWLEEQG
                     ISRVSLSMADEGDTVLAFAVAEK"
     gene            complement(9340..10068)
                     /gene="pdxJ"
                     /locus_tag="ACSIGJ_001542"
     CDS             complement(9340..10068)
                     /gene="pdxJ"
                     /locus_tag="ACSIGJ_001542"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HHQ1330376.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQDKIAEFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQIRQFERIQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(10121..10864)
                     /gene="recO"
                     /locus_tag="ACSIGJ_001543"
     CDS             complement(10121..10864)
                     /gene="recO"
                     /locus_tag="ACSIGJ_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HHQ1330377.1"
                     /translation="MSEYRVNHEPVFMLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSVSWYGSQELKTLHRAEWVGGWRQPQGRALFGGLYVNELVLK
                     LTAREDPVPELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRNTA"
     gene            complement(10901..12028)
                     /gene="pheA"
                     /locus_tag="ACSIGJ_001544"
     CDS             complement(10901..12028)
                     /gene="pheA"
                     /locus_tag="ACSIGJ_001544"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HHQ1330378.1"
                     /translation="MLECTANYRSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDDCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     RLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKAIGSYPTAVL"
     gene            complement(12067..13272)
                     /locus_tag="ACSIGJ_001545"
                     /pseudo
     CDS             complement(12067..13272)
                     /locus_tag="ACSIGJ_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
     gene            complement(13388..14263)
                     /locus_tag="ACSIGJ_001546"
     CDS             complement(13388..14263)
                     /locus_tag="ACSIGJ_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HHQ1330379.1"
                     /translation="MFYQILALIIWGSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANASGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATFA
                     AGRLSRRDAQNGNAV"
BASE COUNT         3409 a         4197 c         3936 g         3171 t
ORIGIN      
        1 gttgcggcaa tctttcccaa actcccttag tcttctaaaa tacaaacctt gcggcttgtt
       61 gccctctctc taactctctc ccgcagggag agaggacggt gcggctgttg gggttaaggt
      121 ttatgcaaac taatacggct tgctgccctc tctctaactc tctcccacgg ggagagagga
      181 ctatgatgcc caccggcggc ttgctattag gaatatctga atcgtcattc ccacgaaagt
      241 gggaatctag aacgtcaaat ctcaagaaac cgttttatcc gataagtttc tgttccgaca
      301 ggtccggatt cccgcctgcg cgggaatgac gaatccatcc atacggaaac ctgcatcccg
      361 tcattcccac gaacctacat tccgtcattc ccgcgaaagt gggaatctag ttttttgagt
      421 ttcagtcatt cccgataaat tgccttagca ttgaaggtcc ggattcccgc ctgcgcggga
      481 atgacgggat ttgaggtttc tgtttttaat tttctgtttt cacgggaatg acggtttaaa
      541 agttacccga aacctcaaaa aaaagctgtg ttttaatata gtgttgatag acgtacttgg
      601 cctccatgta ttcaatcgtg gaaatctata tcttcgtcct cgccgaaata gtctatgccc
      661 gatatacaat tttgatacac aaacttggaa atatgggtat cgtcgccgga gcgatagaat
      721 gcggacagtt atatatatac ggttttttta ggggagcggg cagatgaagg gcggattttg
      781 aattaacccc atgcaaattg acttttgcgg gcggttttac cgcccatata tttacaaaag
      841 ccaaattttt aaacatatat ccttgatata tacacggcgt aaacatatac tggaaacatc
      901 tttaaatttt ccgaaatttt aaacatgagc aactggaaac ccgatattcc ctataacgat
      961 ttaccacccc tgccgccaaa acaggatatt gaaagcaaaa ccatcctgaa acgttgtata
     1021 gccgcccgtg catcccttgc ccgtttaaag caggcggcag aattgatacc gaatcaagcc
     1081 atgctgatta acacccttcc tgttatggaa gcccgtgcaa gttcggaaat tgaaaacatc
     1141 gtaaccacca cggacaagct gtttcaatcc ctgcaaatgg atacggaacg gcaagaccct
     1201 gccacgaaag aagccctgca ataccgcacc gccctgtttg caggctatga atcactggcg
     1261 agccgccctt tatgcacaca aaccgccatc atggtctgca acgccatcaa gcacccctac
     1321 gaaacggcca tccgcaaaac aggcggcaca gccctaaaag gaggtaacag cggaaatgtt
     1381 gtctataccc cgcccgaagg agaagaaacc atacgcggca agctggcaaa ttgggagcgg
     1441 tttattcacg aaagcggcga tttagacccg cttgtcatca tggcggcggc acattaccaa
     1501 tttgaagcca tccacccgtt tacggacggc aacgggcgga cggggcgcat attgaacagc
     1561 ctgctattga ttgaaaaagg gcttttggat ttgcctattt tgtatttgag ccgctacatc
     1621 atcgaaaaca gggcggacta ttaccgcctg cttttaggcg taaccgaacg gcaggattgg
     1681 gaaagctgga taatctacat cttagacggc gtagccgaca ccgccgattg gacggtatcg
     1741 aaaatagatg cgatacgctg cctgttcgag cagacacgtc aacacatacg gacacacgca
     1801 caaggaatct acacgcacga actggtaaat cttctgtttg agcagccata tacacgcatt
     1861 gccaacctag aagcggcagg gatagccaaa cggcagacgg cctctaagta cctgaaagag
     1921 ctttcaggca taggtgtgct gcaagaaatc gccatcggca gggacaaact attcattcat
     1981 ccgcgcctaa tggaactatt gcggggagag ggcaacagct ttacttcatt ctaaccccct
     2041 cttcatccct acatgactaa cacgaaacag ggattttgac acccgaaccg gtaccccttg
     2101 tattttcccc gcgaaaagcc ggcatccgcc cgcgcatcat gggagcaaca aaacccctgc
     2161 ctaaaatttt gacttgtgca aattaggggt atatttgggg ggtatattga aaaatggcta
     2221 aaataaaatg tttaataaac aaaatgttga aacttaattt cgatagagcc tctgcatatc
     2281 gtattgaggc gttaatggaa tttgagaaag ctatttttaa ataagaaaag gtaactatta
     2341 aatagctacc tttctaaaat taaatatcaa cacatcgtta aaacacagcc aaaaaaaacc
     2401 gaaaccgaac ggtccggatt cccgcctgcg cgggaatgac ggatttcaag attacggtat
     2461 tgtcggggat gatgggaaac ggtgggaatt gtgtaaaaaa tgccgtctga agcctttcag
     2521 gcggcatttg cggcattcgg acatttaaaa atcccgtcat tcccgcgcag gagggaatcc
     2581 gaatacatcc gcacagaaac ctgcatcccg tcattcccgc gaaagcggga atctagaacg
     2641 taaaatctaa agaaaccgtt ttatccgaca agtttccgca ccgacaggtc tagattccca
     2701 ctttcgcggg aatgacggga ttttaggtct ctgtaatctc gggcagcttt ccgttatcgt
     2761 tttaacccaa agacaccatt tcaatctgct ccatcgtcct gcccagaaaa cgctcgccga
     2821 tggttctgaa tttcaaagga atatcgctga cgtaaaaacg gtagtcggga ttgttgttgc
     2881 cggtatcgag caatccttcc tgtgcaagga cgcgtgcggt ttcttcggcc gtcgtaatcg
     2941 cggaatcgac caacgcgaca ttgtgcgcct ccctgccgat taagggcttg agcaagggaa
     3001 agtgcgtgca gcccaacacc agcgtatcga tgccgtctgc aagcaatggt ttgaggtatt
     3061 cgcatacggt caggcgggta acttcgtgtt ccaaccaacc ttcctccacc aaagggacga
     3121 gcagcggcgc ggcctgcgtg cggacgagcg tgtcggggtt gtccctgtgg atggcgcgcg
     3181 cataagcatt gctgttgact gtcgtattgg tggcgatgat gccgatttta ttgttgcgcg
     3241 tcgttgccag cgcggctttg gcgccggcgg aaatcacgtc caaaacgggc atattgccgg
     3301 ttttttgacg gattttccgc cccgcaaccg ccgcaatcgt gttgcacgcg ataaccatcg
     3361 ccttgacatc gtgtcccaat aaaaaatcga caatctgcat cgagaaattt tcgatggtcg
     3421 ccttggattt cgtcccgtaa ggcacgcgcg ccgtgtcgcc gaaataaatg atgttctcca
     3481 tcggcagccg ttccatcagc gcgcgcacat tggtcaaacc gccgattccc gagtcaaaaa
     3541 cgccgatggg ccgctgcctg ccgatatttt ccatcttttt tccaatccgt cctaaacata
     3601 caatccgcat attgtacacg cgccgctttt tcttgacagc catgccgtct gaaagcagga
     3661 tgcgtatttg gctgttataa tgtacggcaa tgccgccgcc gggcgcacgg ttttccgacc
     3721 gcctgccctc cgatccgcca cataaaagtt gtaaagcccc tatgtctgat ttttcccccg
     3781 tttcccgatt ccttgccgac gaagccgcca cactcgattt gggcgcggcg tggtcttccc
     3841 gtttaaacgc accgctggtc atttacctcg aaggggattt gggtgcgggc aaaaccacgc
     3901 tgacgcgcgg catcctgcgc ggattgggtc accagggcgc agtcaaaagc ccgacctacg
     3961 ccatcgtcga atcttatccg ctggaacgct tcgccctgca ccatttcgac ctctaccgct
     4021 tctcgttccc cgaagaatgg gaagacgcgg ggcttgacga gctgtttgcc gcaaacagcg
     4081 tctgcctgat cgaatggccg caacagggag gcgaatttac cccgcctgcc gacatcaccg
     4141 caacattgac acacggcggc ggcggcagaa aatgcctgct gaccgcccat accgaacgag
     4201 gaagcgaaag cctgccgcta tgaccaaact gacacgaaga caaatcatcc gccgcaccgc
     4261 cggcacactg ttcgccctaa gccccatcgc atccgccgtt gccaaaacgg tacgcgcccc
     4321 gcagtttacc gccgcacgga tatggccgtc gcacacctac acccgcctga cgctggaaag
     4381 caccgccgcg ctcaaatacc agcacttcgc gctcgacaac ccgggcaggt tggtcgtcga
     4441 tatacaaaac gcaaacatca ataccgtatt gcacgggctt tcccaaaaag ttatggcgga
     4501 cgaccccttt atccgcagca tacgcgcggg tcagaacacg ccgaccaccg tccgcctcgt
     4561 catcgacctg aaacagccca cccacgcaca agtcttcgcg cttccgcccg tcggcggctt
     4621 taaggaccgc ctcgtcgtcg acctctatcc gcacgggatg gatgccgacg atccgatgat
     4681 ggcgctgctc aacggcagcc tgaacaaaac cctgcgcggc tctccggaag ccgacctcgc
     4741 ccaaaacacc acgccccgac ccgggcgggg caaaaacggg cgcagacccg tcatcatgct
     4801 cgatccggga cacggcggtg aagaccccgg cgccgtcagc ccgggcggtt tgcaggaaaa
     4861 acacgtcgtc ctctccatcg cccgcgaaac caaaaaacaa ttggaagcct tgggttacaa
     4921 cgtctttatg acgcgcaacg aagacgtgtt catcccattg ggcgtgcgtg tcgccaaagg
     4981 gcgcgcacgg cgggcggacg tattcgtctc catccacgcc gacgctttca ccagcccctc
     5041 cgcgcgcgga acgggggttt atatgctgaa caccaaaggc gcgaccagct ctgccgccaa
     5101 attcttggaa caaacccaaa ataatgccga cgcggtcggc ggcgtaccga ccagcggcaa
     5161 ccgcaacgtc gataccgccc tgctcgacat aacgcaaacc gccacactgc gcgacagccg
     5221 caaactcggc aaactggtgc ttgaagaatt gggcaggctc aaccatctgc acaaaggcag
     5281 ggtggacgaa gccaatttcg ccgttttgcg cgcacccgat atgccgtcta tcctggtcga
     5341 aaccgccttc ctgtccaatc ctgccgaaga gaagctgctg ggcagcgaat ccttccgtcg
     5401 gcagtgcgcc caatccattg cctcgggtgt ccaacgctac atcaatacat ccgtattgaa
     5461 gcggggttga ttgaaaaggc ggtgtttcaa aatatagttg tcggatgaag gcaggcataa
     5521 acctacttcc gccccaaacc gcgtttttat gatgaaaggt atggataaat tgagatacca
     5581 acgggatttt ttaaatatcc gccccatttt tacagccggc gaacaagaat acctgaccga
     5641 attgtccgat cggcttcctt tatctgtttt aacggactcc gtgcgtaata ttgaagaaat
     5701 cggtatcgat tttgtgtaca gtcctgccaa attggaagga aacacttata atcaatacga
     5761 tacgcaggca ttgcttaaac tcgggcaaac cgcaggcggg aagttatatt cggatgccgt
     5821 aatgttaatc aacctgaggg aaagttaccg gcacctgctg tccggtttgg acagccccaa
     5881 gccgtttgac tggttggatt tcttaaaaac cacccacagc ctgatttcgg aaaacttact
     5941 ggaaaaaggc tccggcggcg tagtccgccg cgacagcgtt actataagcg ggacagacta
     6001 caccccttta tccaacccgc aaagtttgga tacggaattg aaatggttgc ttcaagaagc
     6061 ccccaaaata gaaaatcctt tcgaccgcgc cgtttatctc cataataatt tggcgtatct
     6121 ccgatatttt aaggactgca ataaacgtac tgccagaaac tgtatgacct tgtcgctgat
     6181 gcgctccggt ttttttcctt gcgtattctc ccccgacagt tatcccgcct atgccgaggc
     6241 tgtcgtagcc tattacgaaa ccggcgatta cggtttattc aagaaatatt ttatttcggc
     6301 atacgaaaat accgtaaata agtacggtcc ccagcctgat gtggatattt tccgaaattt
     6361 ctccatttaa aaagccgtct gaacattttt cagacggcat ttttgtacac tttcctcaac
     6421 ggttcgatcc ctgcaccata tcgatcagga acaggcggca gtcgatgttg ccgttataaa
     6481 gcgggatttt ccgcttgggc agcaggcgca tgaatttggg catttccctg tcgccggtaa
     6541 acatgccgac ccgccagccg gcatagtgtt ttttcaacca cgtacccaac tgcggataaa
     6601 gtgcctgcaa agcgcggact tcctcaaggc gcacgccgta gggcggattg gacaccatga
     6661 tgccgttttc gccgttcgga cggacggact gcgcgtcggc aacgctgaag gaaacgatgt
     6721 cgtccgctcc ggcattgtgt gcgttgtcca atgccgtctg aacgatgcgg cggccgttgt
     6781 cgctgcctgc aatcggggcg cggacggggc gggtttgcgc ttcggcgcgg cggcgcaaat
     6841 ccgcccacag cgttttatcg aaattttgca gtttttcaaa accgaagcgg cgcatcatac
     6901 ccggcgcgcg gccggcggca atccaagcgg cttcgatagc aatcgtgccg ctgccgcaaa
     6961 acgggtcttg aaacggctgc gtgccgtcgt agcctgccga gagcagcagc ccggcggcaa
     7021 ggttttcgcg cagcggggct tcgccggtat ccaggcggta gccgcgtttg aacagggctt
     7081 cgcccgaagt gtcgatgaag atttcgacat tgcgttcgtc caaaaaggcg tggatgcgga
     7141 catcgggcgc ggctttgtcc acgctcggac gtgcgtcgta aatgtcgcgg aaagcgtcgc
     7201 agacggcatc tttgacggtc aggccggcaa attggaggct tttgacgttg gcgcgttttg
     7261 cctcgatttt gactttgaac gtctgctgca aggtaaacca gttgaaccag ttgatgtttt
     7321 tggcaagttt gtagatgtcg cgctcggttc ggtatgtgcc tttggtcagg cgcagcagga
     7381 tgcggccggc ggtgcgcgag tgcaggttgg cggcgtaggc ctgttccaat ccgccacggc
     7441 aggaaacgcc gccgtcaaac gcccgaacct cggtgcagcc gaggctttcg agttcttgcg
     7501 ataaaacggt ttccaagccg cgcgggcagg tggcgaaaag tgtatataaa gtattcatag
     7561 cgtgtccttt ccggacggat gttccggcaa tggcggattg aaaggtttat tcggcggtaa
     7621 atgccgtctg aaatgtccgg ctgccgattg taatgtccgc cgtaaaatcg cgcctgcctt
     7681 cgacacagac gggcaggcgg atgcgtgctg cctgccaatt ccccgacggt tgccgctcga
     7741 acatatagcg gttgaaaccc atatccatat ttttcatact gaagctgatg ctgacctgtt
     7801 ccgtgccggc gggcgcgtgt tcgatataaa tatcaaacgg ttttttggtt gaaacggcgg
     7861 cggcgcggac acggcttccg tccggcagcg tgcaaccctc ggtcaaatcg cattgcgccg
     7921 ccacggcttg cggctgctgc gcctgccacc attgcaacaa aacgagcttg aaggcggcaa
     7981 aggcagtcag cagcagtacg gcaagcagta atttgcggtt tttattcatg attcttccct
     8041 attgtttcat cccttaccac cgcgtgcgcc cccaatccga gccatagttt tccataacgt
     8101 gcaaccgtct gtccgtttgc tgcaagtacg agcggcacgg atacgccctg ccgcagggct
     8161 tctgccgcct cttgtgcctg aacatcatcg cacacgcgcc acacgaccac atccgcgccc
     8221 ttttcctgcg cctgccgcca ttgttcgcgg gtctgcacca tcatccacac gctgccggcg
     8281 tgttcggacc tgtcctgcca ttgtgccgcc tccatcaaaa cgttcgcacc gcttccccct
     8341 gccgaaccca aaggcaggac gcggtacgcg cccatactgt tttcgccgta caaacctgtt
     8401 ttcaggctgc cgtacaaacg gcgcgggacg gacagcgaac gcaaaagcgc accgttggca
     8461 ggcagcatgg gggcaacggt aaaatcaccc gccttctgcc aagaccattc ctgcccttcg
     8521 cgcgattgcg gtccgccctc ccattgatcg gggtttaccc ataagaattt caggcagacg
     8581 cgggcgtgtt cgtaggaatg gattttggtc agccaaggcg tggcggcgag gatgcggatg
     8641 ccgagttctt ctccaaactc gcgttgcagg gcttggaagt cggtttcgcc cgcctcgacc
     8701 ttgccgccgg caaattccca atatccggca tagggcttgc cttcggggcg cgagccgagc
     8761 aggtaattgc cgtctgaatc gagcaggatg ccggcaacga cgcggataag ggggcgggta
     8821 tcttggatca tggcgggtat gcggttggtc ggtcggaatg caaatgaggg aagggcaatg
     8881 ccgtcaacgg gtttgccgaa tttcagacgg cattattttt cggcaacggc aaacgccaac
     8941 acggtatcgc cttcgtccgc catactcagg ctgacgcggc tgatgccttg ttcttccagc
     9001 cattcggaca gggcggggcc gtagaaaaat tcgggcttgc ccaatgcgtc gtgcccgatg
     9061 ccgatgttgc agaaggaaac cgcgcctcgt atgcccgtgc cgacggcttt ggcgaaggct
     9121 tctttggcgg caaagcgttt ggcaaggtag ttgacgggtt tgcccgcctg cggaaattca
     9181 agcagctctt cgggagtgag tatgcgccct gcaaacgcct gtccgaattt tttgtttaag
     9241 cggatgatgc gcttgaggga aacgatgtct gtaccgatgc cgtaaatcat atttgcgctc
     9301 ctttttcgtg aaatgccgtc tgaaacggtt tgcctgccct taaggcagca gccttgccct
     9361 gaacatcgcc tccttcattt ggcgcacggc ttcgggcagt ccgaggaaga gggcttgggc
     9421 aatcagcgaa tgcccgatgt tcagctcgcg gatggcgagg atttgggcga tgggggtaac
     9481 gttgtgtatg gtcagtccgt gtccggcgtt gacgaccaag cccaaatcgc cggcaaaatg
     9541 cgcgccgttt tggatgcgct cgaactgcct gatttgttcg gcgtggctgc gcgcgtcggc
     9601 atacgcgcct gtgtgcagct cgacaacggg cgcgccgaca tcataggcgg cttggatttg
     9661 cctgtcgtcg gcatcgataa acaaagacac gcggatgcct gcgtcggtca ggattttggt
     9721 gaactcggca attttgtctt gttgcgccaa tacgtccaaa ccgccttcgg tcgtgatttc
     9781 ctgacgtttt tccggcacga tgcacacgtc ttccggcatc actttaagcg cgttttcgag
     9841 catttcttcc gtcaacgcca tttcaaggtt caggcgcgtg cggatggcgt ttttgacggc
     9901 gaacacgtcc gcgtctttga tgtggcggcg gtcttcgcgc aggtgcatgg taatcaggtc
     9961 ggcaccgtgc gtttcggcaa ccagtgccgc ttccacaggg ctgggataag tcgtgccgcg
    10021 cgcgttgcgg acggtggcga tgtggtcgat gttgacacct aaaagcatga tgtttccttt
    10081 cctcttcgtt tttctgtttg gcggtatggt gcgggcgcag ctacgcggta ttgcggtcaa
    10141 actgccttat ctgttccaac acctgccgcg atttcagccc ttcgggcaac aggtggcgga
    10201 taaaaagccg tgtaattttc aatgcctgtt gcaggctttc ggcggtgcgg aaactgcctt
    10261 cgcgcaaatc gatcaggctt tgcccttcga caattgcgcc ggcttcgtgc gaaagcgcgt
    10321 ctgcgtcctc gcatacgggc atgaccgcct cttcgggcat cagccggtag gttttgtccg
    10381 ccaaaatatc gccgcccgtg ccgtcggcgt gcaaatcggg ggcaacgccc aaggcattga
    10441 gcagcttcca ctcgaagcgg cgcaaatcgg caatgtggtt ggcttcacga cacaccgcct
    10501 ccatcacttt tgccaacgcg tcgtacagtt cgggcaccgg gtcttcgcgg gcggtcagtt
    10561 tcaacaccaa ctcgttcaca tacaatccgc cgaacaacgc cctgccctga ggctgccgcc
    10621 aaccgccgac ccattcggcg cggtgtaggg ttttgagttc ctgactgccg taccacgaca
    10681 cgctgacggg cacgaacggc accaatacgc cgcgcagctc gctctgcctt ttgcgcgcgc
    10741 tgcgcgccag caaagccaca cgcccgtaac ggcggctgaa tgcttcaacc cgcaggctgc
    10801 tttcgcgcca gggcgaagat gccagcataa aaacgggttc atggttgact cggtattcgg
    10861 acataatcgt tgtggaaatg ccgtctgaac gctgccgcga ctacaaaacg gcagtcggat
    10921 acgaaccgat ggctttgaca aacgaagcgc gttcgcccaa gcgttccaat gccgtctgaa
    10981 tctgcgcgtc ccggcggtgt ccttcgatgt cgatgaagaa caggtattcc cacaaaacgg
    11041 atttgctcgg acggctctca aacttggtca tggaaatacc cgattcggtc agcggttgca
    11101 gcagcgaggc aaccgcgccg gcgcggttgg gcgcggaaac ggccagcgag gttttgtcgc
    11161 tgccgcttgc accggtttcg tgatgtccca tgaccagaaa gcgcgtggtg ttgttcggtt
    11221 cgtcttcgat gcactcggca accatatcga gtccgtaaat ttccgccgcc gtgcgtccgg
    11281 cgatggcggc aaccgtaccg tcgtccgatt cggcaaccag ccttgcggct tcggcattgc
    11341 tggacacggc aatccgttcg gcattgggca gacgcctgcc caaccagtcg ttgcactgcg
    11401 ccaacgcctg cgcgtgcgaa aatactttgg cgataccctc ggtgctgccg ttgtttttgc
    11461 gcaacaggtt gtggtggatg cgcaaaacga cttcgccgca cgcctgcaac gcggtaacgg
    11521 caagcaaatc caatgtgcgc ccgaccgagc cttcggtcga gttttccacg ggcgcgacca
    11581 gataatccgc ctgacgggtt tcaacctgtt tgaagcagtc gtctatggtc ggacacgcca
    11641 ttgtatgcgc ggcgtgtccg aaatgcttga ttgccgcctg ttgggtaaac gtgccctgcg
    11701 gccccagata ggcaatggtc agcggacgtt cgacggcgag gcactcgctc atcacttccc
    11761 gaaacaggcg tgctaccgat tcgtcgggca gcgggccttt gttcaaatcc tgaatgcggc
    11821 gcaacacggc aacctcgcgc tccgggcggt ataccgcgcc cgtgcctttc agctcgccga
    11881 tggcgtgcgc gtgttgcgcg cgttcgttga gcaggcgcag gatttccgca tcgatggtgt
    11941 cgatggcgtt gcggtgggga aggaggagtt cgtcgatagt ttgggacata atttcgcctg
    12001 aacggtaatt tgccgtgcat tctaacatca aatgccgtct gaagcggttt cagacggcat
    12061 tttccgttaa aggtattcgc tttgcccgtt ttctttccac gcacgatgcg agcagagcca
    12121 caaaagcgca atgccgcaag aggcggacgc ggtcatggtt gccgccatca cggttgccgc
    12181 catgaccacg cccgcgccga ttaaggaccg gaatacaccc gacacggcgt tcgcgctgcc
    12241 gccctcttct ttgaaataag acataaagca tgcctgcgtg tccgcaccga ccaggccctg
    12301 cgtaccgacg gaaaacatca cgcacgcgac cggcagccaa aacgggggca acccgaaaaa
    12361 cagcacggcg gcgagttggg acgggttggc ggcaaattgg acgacaatcc cccgcagcag
    12421 gatgctttgc ggatgcgcgc cggttttaag ccgccacgcg gtaacgcggc tgaaaaacat
    12481 catcgtgatg atgttgagtg caaacaccca tgcgtaccgg tgcggcgtaa cgtggtagag
    12541 ctgccggtac acgaaggaag attcggtcag aaaggcgaac atcgaaccga agctgaatgc
    12601 ctgaaaaaac agataaccca tcgcggcacg ggttttcaat acgcgcttga accgccccgc
    12661 caccagcccg aacacatccc tgccgatttt gccgccgacg gcgggattgg gcaggaaata
    12721 ctgtaccaaa ccgggcagca ccggcgaata cgccgccaag aaaacgaaaa tcgcccgcca
    12781 tccgcccaat ccctgcaaca atgcgccgac catgggtgcg gccagcggca caaccatcag
    12841 aatgatgccg ataagggcaa acatctgcgc ggctttgcgt ccggaataat aatcgcgcac
    12901 catcgcaccg acgatgacta cagccatgcc tgcgccgaac gcctgtaccg cacgcaggtt
    12961 aaggagctgt tcggtactcg aagcaaatac gatggcggca acggcaaggc aatatacaat
    13021 caaaccggtc agggcgacgg gtttgcgccc tttgatgtcg gacaccgcgc cgccggccac
    13081 ttgcccgaac gccgtgccga acataaacaa actcagactc tattcgatac ggtggatatc
    13141 cgcgttcagc ggctgcgcca tttcgggaat cgcgggcagg taggcatcgg tggaaaacgg
    13201 catcagcgcg accagtaccg ccatcaaaac cgccatcagt tttccgctca tcggaggaag
    13261 ggtcggagac atggtttttc cgttcaaatc aaataaagaa accgtcgcaa acgacggtcg
    13321 gcacgggcgc aagtttaaca tatcggacgg cggggcaaaa ccgaaggata tgccgcccga
    13381 aaccgcttca gacggcattg ccgttttgcg cgtccctgcg cgacagccgg ccggcggcga
    13441 aagtggcggc gatgacgaca aacacgccca aggcggacac gggcgataaa tgttcgccca
    13501 aaatcaaaac cgccaacagc acgccgacga cgggttcgag cgaaatcaac agtcccgacg
    13561 cgttggcagg aacacggctc atccccttgt tccacagcca ataggcgtac cagccgcacc
    13621 ccaaacccaa atacaacagc gacaatacca tcccgacgct ccagtccacg gtataacttt
    13681 gcgccaaagc aagcgaaaac ggcaggcaca tcaacgatgc ggcggcaatg gaaacagatg
    13741 tgaatgccgg tgcgccgatg cgggcaatca gcctttgcgt cggacgcata gcggcacaaa
    13801 agcccgcgcc cgccaacaac accagcaggc agccgaacca gccgacttcg ccgccctctt
    13861 ccgcaccgcc cgccatcagc agcgcgacac cggcaaatgc cgccgcgccg catatccagt
    13921 ggtaggcacg cgctttgtcg ttgaagaaaa agtgtccgac aaacaccatc agcagcggct
    13981 cgagtccgac aatgaccgat gcgctggcgg cggaagtgta tttcaacccg acaaactgaa
    14041 gcagcagggt cagcacatag ttgacgaacg acacaatcag caacggcttc cattcctcac
    14101 gcggaatctt gccgacatga cggcggcagg cgggcagtgc aggcagcgcg gcaatcagca
    14161 ggcgcacgcc gaccatcaat gcgggatcga tgccgccata gacatatttg gcggcaataa
    14221 acgagctgcc ccagataatc agggcaagga tttggtaaaa catagtgagt ggtaaagtca
    14281 gatggtcata caaaagtata gcggattaac aaaaatcagg acaagaagga atgcccgaac
    14341 cgtcattccc gccacttttc gtcattcccg cgaaagcggg aatctggaat ctcggacttt
    14401 cagataatct ttgaatattg ctgttgttct aaggtctaga ttcccgcctg cgcgggaatg
    14461 acggctgcag atgcccgacg gtctttatag tggattaaca aaaatcagga caaggcgacg
    14521 agctgcagac agtacagata gtacggaacc gattcactcg gtgcttcagc accttagaga
    14581 atcgttctct ttgagctaag gcgaggcaac gccgtactgg tttttgttca tccactataa
    14641 cagcaaccct gtcgccgtca ttcccgcaaa agcgggaatc cagtccgttc agtttcggtc
    14701 atttccgata aat
//
LOCUS       DBJGGI010000052        14240 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.052, whole genome shotgun sequence.
ACCESSION   DBJGGI010000052 DBJGGI010000000
VERSION     DBJGGI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14240
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.052"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(12..920)
                     /gene="hslO"
                     /locus_tag="ACSIGJ_001547"
     CDS             complement(12..920)
                     /gene="hslO"
                     /locus_tag="ACSIGJ_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HHQ1330380.1"
                     /translation="MNQTAINRADARTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRCALGELLAAGVLLSGNLKNEGTLIVQVQGQGKLKMLVAEATSDRTVRATARWDET
                     AEIADDESLGDLLGGNGVFVLTLQPKDGEPWQGVVPLEGGSIAQMLVNYMKRSEQLDT
                     HIALSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAEELAELDAQHVLYRLFH
                     ETPPRVFEPETFESSCTCSRGKVSDMLLMLGGEEVGGVVAEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            1165..1833
                     /locus_tag="ACSIGJ_001548"
     CDS             1165..1833
                     /locus_tag="ACSIGJ_001548"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HHQ1330381.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPVNRAPARRAGNADELIGSAMGLNEQPVLPVNRAPARRAGNADELIGSAMGLL
                     GIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGAPVARSELQPGDMV
                     FFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARRIKKNDPS
                     RFLN"
     gene            1852..2070
                     /locus_tag="ACSIGJ_001549"
     CDS             1852..2070
                     /locus_tag="ACSIGJ_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330382.1"
                     /translation="MSMPEMPKWYGDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRAYLLALIEGLENPYRKV"
     gene            2215..2559
                     /locus_tag="ACSIGJ_001550"
     CDS             2215..2559
                     /locus_tag="ACSIGJ_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HHQ1330383.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            2559..3251
                     /locus_tag="ACSIGJ_001551"
     CDS             2559..3251
                     /locus_tag="ACSIGJ_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HHQ1330384.1"
                     /translation="MSEILRQPGILLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILGIDYAVYHNAAQFIDFRLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLPLASKSVTNPIAIEITSSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            3318..4538
                     /gene="argJ"
                     /locus_tag="ACSIGJ_001552"
     CDS             3318..4538
                     /gene="argJ"
                     /locus_tag="ACSIGJ_001552"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HHQ1330385.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDQHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTCDEARQAAYAAARSPLVKTAFFASDPNLGRLLAAIGYADVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKDEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            4598..6007
                     /locus_tag="ACSIGJ_001553"
     CDS             4598..6007
                     /locus_tag="ACSIGJ_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HHQ1330386.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWIALPPGLPVIVWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLAAGFI
                     QVAIQGNNPYFSGFNGGVLENILMWIVAAGLVCGAAGGIFARMLHRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPVGLAAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRRRVLQETAAQTG
                     NAPARPQAANSKTGMPSEN"
     gene            6195..7586
                     /gene="hrpA"
                     /locus_tag="ACSIGJ_001554"
     CDS             6195..7586
                     /gene="hrpA"
                     /locus_tag="ACSIGJ_001554"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HHQ1330387.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKIEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLEEIKKTVAENQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKCRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHVEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMASLNLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEVV"
     gene            7654..9156
                     /locus_tag="ACSIGJ_001555"
     CDS             7654..9156
                     /locus_tag="ACSIGJ_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HHQ1330388.1"
                     /translation="MNIKEFMSNYTNHPVLFIGTGMSLRYLDNSYTWDGLLSKIAIDL
                     FGDDREYLNIKSRYCEDGRFQYEEIAEELQSKFDKVLENDPDGRFKEINDKFFENMRA
                     GNTLSRFKIYISTLLSQLNYKDNSNTELSELKKARKNVGSIITTNYDKLAQDIFEFNP
                     LIGNDILLSNPYGSVYKIHGCVDDPSKIIITKKDYEKFKEKYELIRAQLLSLFIHNPI
                     IFLGYNVGDENIKEILKTIFTYVEPNSPSANKIRRNFLLVEYEPESNNEDIVEHDIDI
                     TGFSTIRINKIKTDNFSQIYKALAELTLPISAMDVRKFQSIAKEIYTGGNIKVSFTED
                     MDNLNNSDKVVAIGSTKTISYNFQTTSEMMSNYFKIIEEENSQLLKLIDKHSIASTQY
                     FPIYGFSRICSDIHKEAVLKRQQKEKLDHFIEEINRRCKNNHSSIQSILDDENISDTY
                     KNDAIAWGIWNNQLSEDEVENYLKNFVNKKNTHYKRLLCMFDYKKYADTV"
     gene            9383..10960
                     /gene="lpt3"
                     /locus_tag="ACSIGJ_001556"
     CDS             9383..10960
                     /gene="lpt3"
                     /locus_tag="ACSIGJ_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HHQ1330389.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FVIAALYLFARYKATRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPALWGVAEVVLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSKIGQGSIQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIADK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHDQYVRQDIYNQGTVQPDSYIVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRNGKAEYVYPQ"
     gene            11026..>14240
                     /locus_tag="ACSIGJ_001557"
     CDS             11026..>14240
                     /locus_tag="ACSIGJ_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HHQ1330390.1"
                     /translation="MQNMKNQVRGSGMDARSNPANVSDGLQNSSGHIGTNTRYRLTKL
                     GEQMARLPIDPKIARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHER
                     FTDKQSDFLTYLNIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQT
                     AIEMGLTTKEAAFRRPPEVKQLTSSENAGDQDLSAKLKQKQLDKKQHRTQIRATKEAG
                     YEQIHRALLTGLIANVGMKSPDSNDYTGARGSRFHLFPASALFKAKPKWVMAAELVET
                     TRLYARDVAVIQPEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLAVLPRRP
                     VSYGKVAPEEAREIFIRSALVAQEYDLKADFFVHNKKLIKEITELEHKSRKQDVLVDD
                     EALFAFYHERLPDFYMADSVSEGLCPANPQQTTPSPVGEGWGEGKTVAAQTNFSATSA
                     SPLPNPLPQEREQSASVSTVSGSLKTMSCEARLNFCEAKTKTESSLHSQRLSENYTPP
                     FSDGLRPANPQQTTPSPVGEGWGEGKTVAAQTNFSATAANPLPQEREQSASALTVSDD
                     PKPKKQPASQKGRLKPLPLADIRTFEAWLKTAERDNPRLLFLSRDDLMQHAAAHITEE
                     QFPKHWQTADGKFKLSYRFEPHHPLDGVTLTLPLTVLNRISPAALEWLVPGMIREKIQ
                     LQIKALPKQIRRICVPVPEFITQFLSQNPDRNAPILPQLAQAIAKTAGDIRILEQINQ
                     DEWAAFRLPEHCYFNLRIIDDGGQELAMGRDLIQIQQQLGKAATTTFRDNTQEFERDN
                     VTAWDIGILPESIKFARGKQQLTGYLGLQKEKDGRIALRLFDTSAAAGHAHRLGVIEL
                     MKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGEDKLPRNEK
                     AFKEQIKRARSRLPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLLRLRLQTLLAPG
                     FATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKTDGLAKQ
                     GQPVSDGLAAFKWMIEELRVSLFAQELKTPYPVSVKRLLKM"
BASE COUNT         3600 a         4182 c         3428 g         3030 t
ORIGIN      
        1 gttgctcaag attattggac ggtatgtcgg ggcagccctt tggcaacgcc gaccacatcc
       61 tccccgaaca gcgcgttgac atcggtttca tcaaacacgt atttgctgtg gcagaagtcg
      121 caatcgactt cgatgctgcc ttgttccgcc accacgccgc cgacttcttc cccgcccagc
      181 atcaacagca tatcgctgac cttgccccga gaacaggtac atgaggattc aaacgtttcc
      241 ggctcgaaca cgcgcggcgg cgtttcgtgg aacaggcggt ataaaacgtg ttgcgcgtcc
      301 aattctgcca gctcctccgc cgtcagcgtg cgcgccagcg tactgacgtg ttcccatgcc
      361 tcttcatcca acacctcttc aggcagacgc tgcaccagca gcccgcccgc cgcttcatcg
      421 cttgcagaca gggcgatgtg cgtatcaagc tgttcggaac gtttcatata gttcaccaac
      481 atttgcgcga tgctgccgcc ttccaaaggc actacgccct gccagggttc gccgtctttg
      541 ggttgcagcg tcaatacgaa cacgccgttg ccgcccaaaa ggtcgccgag gctttcgtca
      601 tcggctattt ctgcggtttc gtcccaccgc gcggttgcac ggacggtacg gtcggaagtc
      661 gcttccgcaa ccagcatttt cagttttccc tgcccctgaa cctgcacaat cagcgtgcct
      721 tcgtttttaa ggttgcccga cagcagaacg ccggcagcca gtagctctcc caaggcgcag
      781 cggatggcgg cgggatagtt tttctgtttt acaatgtgct gccacacgtt ttccagacgg
      841 acgtgcagcc cgcgcacggg catatcgtcg aagataaagc gggtacgcgc atcggcgcgg
      901 ttgatggcgg tttgattcat gattttctct gactgattgt tcggatggcg gctatatggt
      961 tgcggtcggc gcgaaaacaa ggcggacggc ggatgcgctt cccaaattct caataaatta
     1021 tataaaaatc aacatattaa ctcaatctaa caagccgttt tttgccaaac agccgttttt
     1081 ttatatacaa tcaacaagat attttcgact gatacagcat aacatcgcac ggcggcacga
     1141 tgcctcctgc gcggaaacac cgatatggat tcttttttca aaccggcagt ttgggcggtt
     1201 ttgtggctga tgtttgccgt ccgccccgcc cttgccgacg agttgaccaa cctgctcagc
     1261 agccgcgagc agattctcag acagtttgcc gaagacgaac agcccgtttt acccgtcaac
     1321 cgagcccccg cccggcgggc gggcaatgcc gacgaactca tcggcagcgc gatggggctt
     1381 aacgaacagc ccgttttacc cgtcaaccga gcccccgccc ggcgggcggg caatgccgac
     1441 gaactcatcg gcagcgcgat ggggcttttg ggtattgcct accgctacgg cggcacatcg
     1501 gtttctaccg gttttgactg cagcggattc atgcagcaca tcttcaaacg cgccatgggc
     1561 atcaacctgc cgcgcacgtc ggcggaacag gcgcggatgg gcgcacccgt tgcccgaagc
     1621 gaattgcagc ccggggatat ggtgtttttc cgcacgctcg gcggcagccg catttcccat
     1681 gtcggacttt atatcggcaa caaccgcttc atccacgcgc cgcgcacggg gaaaaatatc
     1741 gaaatcacca gcctgagcca caaatattgg agcggcaaat atgcgttcgc ccgccggatc
     1801 aagaaaaacg acccgtcacg ctttctgaac tgatttccca aggaatacgc aatgagtatg
     1861 cccgaaatgc ccaaatggta cggcgatgac ggacagatcg tatcctgtac cgaaaaggtc
     1921 aaagtgatgt ccgaaaatat ggccgagctg tatcagacgg cacaagacgc gtttgaagac
     1981 gcgctgctga tgggttgcgg cgaacggcag ttgcgcgctt acctgctcgc gctgattgaa
     2041 ggtttggaaa atccctaccg caaagtctga acacgccccg gttgctgcgg cacggtttat
     2101 ccgtgccgtt tttgcgtttg tgcgcggctt cggcttttca gacggcatat ttgacgttat
     2161 gattaaacag ttaacaagat ttatcacaac gccgtcaaaa gacagacaca caacatgaac
     2221 atcatccgcg cgctcctcat catcctcggc tgcctcgccg ccggcgaaac cgccgttttc
     2281 ctagcaggca tcaaactgcc cggcagcatc gtcggcatgg gcgtgctgtt tgcgcttttg
     2341 caggcgggtt gggtcaaaac gtcttggctg caacagctta ccgacgcgct gatggcaaac
     2401 ctgacgctgt tcctcgtgcc gccctgcgtg gcggtcatca gctatttgga tttgattgcc
     2461 gacgattggt tttcgatact ggtttccgcc tccgccagca ctttgtgcgt actgctggtt
     2521 acgggcaagg ttcaccgctg gatacggagc attatctgat gagcgaaatc ctcaggcagc
     2581 ccggcatcct gcttttcctc acgcttgccg tgtacgcgct tgcgattatc gtgcgcacgc
     2641 gcacgggcaa tatcttctgc aaccccgtac tcgtcagcac tatcgtgctg attgcctacc
     2701 tgaaaatcct cggtatcgat tatgcggtgt accacaacgc cgcgcaattc attgattttc
     2761 ggctgaaacc cgccgtcgtc gtgcttgccg tgccgctcta ccaaaaccgc cgtaaaatct
     2821 tcaaccagtg gctgcccgtc atcgtttcgc agcttgcggg cagcgttacg ggcattgtta
     2881 cggggatgta ttttgccaag tggctgggcg cggaacgcga agtcgtcctc ccgctcgcgt
     2941 ccaaatctgt taccaacccc atcgccatcg aaatcaccag ctccatcggc ggcattcccg
     3001 ccattaccgc cgccaccgtc atcattgccg gtctggtcgg acagattgcc ggttacaaaa
     3061 tgttgaagaa cacggtcgtc atgccctcgt ccgtgggtat gtcgctcggc acggcttcgc
     3121 acgcgatggg cattgccgcc tcgctcgaac gcagccgccg catggcggca tacgcggggc
     3181 tggggctgac gttcaacggc gtgctgaccg cgctgattgc gccgctgctt atccccgttt
     3241 tggggttctg aacccgtttc agacggcatt tcaacccatg ccgtctgaac gccgacacac
     3301 tcgcaaggag aaccgttatg gctgtcaacc tgaccgaaaa aaccgccgaa caactgcccg
     3361 acatcgacgg cattgccctc tacaccgccc aagcaggcgt gaagaagccc gggcataccg
     3421 acctgacact gattgccgta gccgccggca gcaccgtcgg tgcagtcttc acgaccaacc
     3481 gtttctgtgc cgcgcccgtc cacatcgcca aatcgcacct tttcgacgaa gacggcgtgc
     3541 gcgccctcgt catcaacacg ggcaacgcca acgcgggtac gggcgcacag ggcagaatcg
     3601 atgctttggc agtgtgtgcc gccgccgccc ggcaaatcgg ctgcaaaccg aaccaggtgc
     3661 tgcccttctc caccggcgtg attctcgaac cgctgcccgc agacaaaatc atcgccgccc
     3721 tgcccaaaat gcagcctgcc ttctggaacg aagcggcacg cgccatcatg accaccgaca
     3781 ccgttcccaa agccgcctcg cgcgaaggca aggtcggcga ccaacacacc gtccgcgcca
     3841 caggcattgc caaaggctcg ggcatgattc atcccaatat ggcgaccatg ctcggtttca
     3901 tcgccaccga tgccaaagtt tcccaacccg tcctccaact gatgacgcag gaaatcgccg
     3961 acgaaacctt caacaccatc accgttgacg gcgacaccag caccaacgac agcttcgtca
     4021 tcatcgccac cggcaaaaac agccaaagcg aaatcgacaa catcgccgac ccgcgttacg
     4081 cccaactcaa agaattgttg tgcagccttg cgctcgaact cgcccaagcc atcgtccgcg
     4141 acggcgaggg cgcgaccaag ttcatcaccg tccgcgtcga aaacgccaaa acctgcgacg
     4201 aagcccgcca agccgcctac gccgcggcac gttcgccact ggtcaaaacc gcctttttcg
     4261 cctccgaccc caacctcggc aggctgctcg ccgccatcgg ttatgccgac gttgccgacc
     4321 tcgataccga cctcgtggaa atgtatctcg acgatatttt ggttgccgaa cacggcggac
     4381 gcgccgcaag ctacaccgaa gcacaagggc aggcggtgat gtcgaaggac gaaatcaccg
     4441 tccgcatcaa gctgcatcgc ggacaagccg ccgccaccgt ctatacctgc gacctgtcgc
     4501 acggatacgt ttccatcaac gccgactacc gttcctgacc cgacacggct tcagacggca
     4561 tacataaaat gccgtctgaa ccgtcggaca acataccatg acctccacat tcccccgccg
     4621 cctcgcccgc aaaatccgcc aaacccgccg cctgtcgcgc aaaagcatcg cctttctgtt
     4681 ccttctggca ggttcggcac tcgtcgccct gaccgcactg ttttttgccc atcttgccga
     4741 ttttgcgctg gaactgaacg ccaaactggt tcaacaatac ccgtggttcg catggattgc
     4801 cctgccgccg ggactgcccg tcatcgtctg gctcacacgc aaattcgccc ccttcaccgc
     4861 cggcagcggc atccctcaag tcatcgcctc actgtcgctg ccctacggcg cacaaaaaac
     4921 gcggctgata cgcctcgggc aaacgctgct gaagattccg ctgacctttt tgggtatgct
     4981 gttcggcgcg tccatcggac gcgaaggccc gtccgtgcag gtcggcgcgg cagtaatggg
     5041 cgcgtggggc gcgtggtgca aaaaacacgg tttggcgttt aagggtatgc aggaaaacga
     5101 tttgatggcg gcgggcgcgg cgggcggttt ggcagcagcg ttcaacgcgc cgctggcggg
     5161 cgtgattttc gccattgagg aactggggcg cggcatcatg ctgcgctggg agcggcagat
     5221 tctgctcggc gtactcgccg ccggtttcat acaggtcgcc attcagggca acaacccgta
     5281 tttttcgggt ttcaacggcg gcgttttgga aaatattttg atgtggatcg tggcggcggg
     5341 gctggtttgc ggcgcggctg gcggcatatt tgcccgtatg ctccatcgcg gtgcggcggc
     5401 gtttgcaccg cgcaagatac gcggcttcat ccgcaaccgt ccgctgctgc tggcggcact
     5461 gatggggctg ctgctcgccc tgctcggcac gttctaccaa ggcaaaacct acggcaccgg
     5521 ctaccacgaa gccgcccaag ccctgcacgg catctacgaa gcccccgtcg gactcgccgc
     5581 cgccaaatgg ctcgccaccg tattcagcta ttgggcaggc atcccgggcg gcattttcac
     5641 tccctcgctg accataggtg cggttttggg cgagcatatc gccgccatcg ccgacatatc
     5701 gcagggtgca aacatcatcg tcctcatctg catggcggca tttttagcgg gcgcgacaca
     5761 atcgccgatt acttccgccg tcgtcgtcat ggaaatgacc ggcgggcaaa gcctgctgtt
     5821 ttggatgctg attgcctgca ttttcgcctc gcaggtttcg cgccagtttt cgccgcgtcc
     5881 gttctaccac gcatcgggaa tgcgcttccg ccggcgcgtg cttcaggaaa ccgccgccca
     5941 aaccggcaat gcgcccgcaa gaccgcaagc cgcaaacagc aaaacgggaa tgccgtctga
     6001 aaattaaaac gcccccgatc aaacgccggc agccgccttg attcaaatac cgttccgctg
     6061 cgcttgaaat ttcagcaaca atgccgtctg aacgacagaa tgcggttttc agacggcatt
     6121 tccccatccc gatattgttc aaacaaaacc gaagcgtttg ctataattgc actttttacc
     6181 gcacacgcac gcccatgttt cccgatttct cccaaaccct ctccaaagac cgccacttcc
     6241 tgcaatccgc cttcaaaaat cccaacaaat acggcggctt gtccaaaatc gaagaaaaat
     6301 accgaaaatc gcacgaaatc ttcttgaaac gtttggccgc attgccaaaa cccgaattcg
     6361 acaacaccct gcccgttcac gaaaaactcg aagaaatcaa aaaaaccgtt gccgaaaatc
     6421 aggtaacgat tatctgcggc gaaaccggtt cgggcaaaac cacgcagttg cccaagattt
     6481 gtttggaact cgggcgtggg gcggcaggat tgatcgggca tacccagccg cgccgtttgg
     6541 ccgcgcgttc ggtggcggag cggattgccg aagagctgaa atccgaaatc ggcagcgcgg
     6601 tcggctataa agtgcgcttt accgaccaca cctcgcgcga tgcctgcgtc aagctgatga
     6661 ccgacggcat cctgctggcg gaaacccaga ccgaccgtta tctcgccgcc tacgacacga
     6721 ttatcattga cgaagcgcat gaacgcagcc tgaacatcga cttcctcttg ggctacctaa
     6781 aacaactcct gccgcgccgc cccgatttga aagtcatcat cacatcagcc accatagacg
     6841 cagaacgctt ctcccgacac ttcaacggcg cgcccgtact ggaagtgagc gggcgcacct
     6901 atcccgtcga aatcctctac cgaccgctga ccggcaaaga cgaagacgac gcagaagtcg
     6961 agctgaccga cgcgattgtc gatgccgccg acgaattggc acgctacggc gaaggcgata
     7021 ttttagtatt cctgccgggc gaacgcgaaa tccgcgaagc ggcagaagcc ctgcgcaaat
     7081 ccacactgcg ccgcaatgac gaaatcctgc ccctgtttgc acgcctgtcg cacgccgaac
     7141 agcacaaaat cttccaccct tcaggcgcaa aatgccgcat cgtgctggcg accaacgtcg
     7201 ccgaaacctc gctcaccgtg ccgggcatca aatacgtcat cgacaccggc ctcgcgcgcg
     7261 tcaaacgcta ttccgcgcgg gcaaaagtgg aacaacttca cgtagaaaaa atctcccaag
     7321 ccgccgcccg ccaacgctcc ggccgctgcg gacgcgtctc agcaggcgtg tgtatccgac
     7381 tgttttcaga agaagatttt aacagccgtc ccgaatttac cgatcccgaa atcgtccgca
     7441 gcaacctcgc cgccgtcatc ctgcgcatgg catcgctgaa cttgggcgac gtagcagcat
     7501 tcccgttttt agaaatgccc gactcgcggt atatcaatga cgggtttcag gtgttgttgg
     7561 aattgggggc agtggaggtt gtctgaaaac aggcagatcg ggcgtttatg cccaacgaga
     7621 accttattgt cactattatt agggattaat acaatgaata taaaagaatt tatgtctaac
     7681 tataccaacc atcccgttct ctttattgga acaggtatga gtttgagata cttagataat
     7741 tcatatactt gggatggttt attatctaaa attgcaatag atttatttgg agatgatagg
     7801 gaatatttga acatcaaatc acggtactgt gaagatggta gattccaata tgaagagatt
     7861 gcagaggaat tacaaagtaa atttgataaa gttttagaaa atgaccctga tggtagattt
     7921 aaagaaataa atgataagtt ctttgaaaat atgagggcgg gaaacacctt gagcaggttt
     7981 aagatttata tatccaccct gctctcacaa ttgaattata aagataattc taatacagaa
     8041 ttatctgaat taaaaaaagc gagaaaaaat gtagggtcaa tcattacaac aaattatgat
     8101 aaattagccc aagatatttt tgaatttaac ccactaattg gtaatgatat tcttttaagc
     8161 aacccttatg gctcagtata caaaatacat ggttgtgtgg acgatccatc aaaaattatt
     8221 attaccaaaa aggattatga gaaatttaaa gaaaaatatg aacttattag agcccaacta
     8281 ttatcgttat ttattcacaa tccaatcata tttcttggat ataatgttgg tgacgaaaat
     8341 atcaaagaaa ttttaaaaac aatctttact tatgtagaac caaactctcc ttcagctaat
     8401 aaaattcgta ggaatttttt actcgtagaa tatgaacctg agtctaacaa tgaagatatt
     8461 gttgaacatg atatagatat aactggattc tctactatcc gtattaataa aatcaaaaca
     8521 gataacttct cacaaattta taaagctctt gcagaactaa cactaccaat ctcagctatg
     8581 gatgtacgta aatttcaatc tatagcaaag gagatttata ctggcggtaa cattaaagtt
     8641 agctttacgg aagatatgga caatttaaat aatagcgata aagtggttgc tattggttca
     8701 actaaaacta tcagctacaa ctttcaaaca acatcagaga tgatgtcaaa ttatttcaaa
     8761 ataattgaag aggaaaattc acaacttcta aaattaatag ataaacatag tatagcatct
     8821 acgcaatatt ttcctattta tggatttagt aggatatgtt ctgatataca taaagaagct
     8881 gtactaaaac gccaacaaaa agaaaaatta gatcatttta ttgaagaaat aaataggcgt
     8941 tgtaaaaata atcattcatc catccaatca attttagatg atgaaaatat ttcagacaca
     9001 tataaaaacg atgcgattgc ttggggaata tggaataacc aactttcaga agatgaggtt
     9061 gaaaattatt taaaaaattt tgtgaataaa aaaaatacgc actataaaag actactatgt
     9121 atgttcgatt ataaaaaata tgcggatact gtctaaactc taaaaaataa ggtcgggcat
     9181 ttatgtccga cctacaaaac tatgcaaaac actaaaaatc aagcacgcag ctcaggcatt
     9241 catgcccaac acaatccgac aaatgacaaa ccactttcag acaacctcgt tttttgaaaa
     9301 acaatctgta aacagatagc tgctgaagaa taccgttgcc gagccccaaa acccgtcctg
     9361 caacttttat tgtgaactgc ccattatgaa aaaatccctt ttcgttctct ttctgtattc
     9421 atccctactt accgccagcg aaatcgccta tcgctttgta ttcggaattg aaaccctacc
     9481 ggctgcaaaa atggcggaaa cgtttgcact gacatttgtg attgctgcgc tgtatctgtt
     9541 tgcgcgttat aaggcaacgc gtttgttgat tgcggtgttt ttcgcgttca gcattattgc
     9601 caacaatgtg cattacgcgg tttatcaaag ctggatgacg ggtattaact attggctgat
     9661 gctgaaagag gttaccgaag tcggcagcgc gggcgcgtcg atgttggata agttgtggct
     9721 gcctgctttg tggggcgtgg cggaagtcgt gctgttttgc agccttgcca agttccgccg
     9781 taagacgcat ttttctgccg atatcctgtt tgccttccta atgctgatga ttttcgtgcg
     9841 ttcgttcgac acgaaacaag agcacggtat ttcgcccaaa ccgacataca gccgcatcaa
     9901 agccaattat ttcagcttcg gttattttgt cggacgcgtg ttgccgtatc agttgtttga
     9961 tttaagcaag atccctgtgt tcaaacagcc tgctccaagc aaaatcgggc aaggcagtat
    10021 tcaaaatatc gtcctgatta tgggcgaaag cgaaagcgcg gcgcatttga aattgtttgg
    10081 ttacgggcgc gaaacttcgc cgtttttaac ccggctgtcg caagccgatt ttaagccgat
    10141 tgtgaaacaa agttattccg caggctttat gacggcagta tccctgccca gtttctttaa
    10201 cgtcataccg cacgccaacg gcttggaaca aatcagcggc ggcgatacca atatgttccg
    10261 cctcgccaaa gagcagggct atgaaacgta tttttacagt gcccaggctg aaaaccaaat
    10321 ggcaattttg aacttaatcg gtaagaaatg gatagaccat ctgattcagc cgacgcaact
    10381 tggctacggc aacggcgaca atatgcccga tgagaagctg ctgccgttgt tcgacaaaat
    10441 caatttgcag cagggcaggc attttatcgt gttgcaccaa cgcggttcgc acgccccata
    10501 cggcgcattg ttgcagcctc aagataaagt attcggcgaa gccgatattg cggataagta
    10561 cgacaacacc atccacaaaa ccgaccaaat gattcaaacc gtattcgagc agctgcaaaa
    10621 gcagcctgac ggcaactggc tgtttgccta tacctccgat catgaccagt atgtgcgcca
    10681 agatatctac aatcaaggca cggtgcagcc cgacagctat attgtgcctc tggttttgta
    10741 cagcccggat aaggccgtgc aacaggctgc caaccaggct tttgcgcctt gcgagattgc
    10801 cttccatcag cagctttcaa cgttcctgat tcacacgttg ggctacgata tgccggtttc
    10861 aggttgtcgc gaaggctcgg taacaggcaa cctgattacg ggcgatgcag gcagcttgaa
    10921 cattcgcaac ggcaaggcgg aatatgttta tccgcaataa gcagcgtgaa aaccaataaa
    10981 gacaaattta gatgatgtcg gggaagatgc ccgaccgaca agactatgca aaatatgaaa
    11041 aaccaagtac gcggatcagg catggatgcc cgatccaacc cggccaatgt ttcagacggc
    11101 ctgcaaaaca gttcgggcca tatcggtacc aacacgcgct accgcctgac caagctcggc
    11161 gaacaaatgg cgcgcctgcc catcgatccg aaaattgcgc gcattttgct ggcggcgaag
    11221 aaacacgact gcatggcgga aatattggtg attgcgtctg cgctgtcgat tcaagacccg
    11281 cgcgaacggc cgctggaagc gcgcgatgcc gcggccaagg cgcatgaacg ctttaccgac
    11341 aagcaatccg atttccttac ctacctgaat atttgggaca gtttccagcg cgagcgcgac
    11401 aaaggtttgt ccaacaagca gttggtgcaa tggtgccgtc aatattttct gtcgcacctg
    11461 aggatgcgcg agtggcgcga gctgcaccac cagcttgccc aaaccgcgat tgaaatgggc
    11521 ttgaccacca aagaagctgc gtttagacga cctcccgaag tcaagcaact gacgtcgtct
    11581 gagaatgcgg gcgaccaaga cctatctgcc aaactcaaac aaaaacaact ggataagaaa
    11641 caacaccgca cccaaatccg cgctaccaaa gaagcgggct acgaacaaat ccaccgcgcc
    11701 ctgctcaccg gcctcatcgc caacgtcggc atgaaatcgc ccgacagcaa cgactacacc
    11761 ggcgcgcgcg gcagccgctt ccaccttttc cccgcctccg ccctgttcaa agccaagccc
    11821 aaatgggtga tggcggcaga attggttgaa accacgcgcc tttacgcgcg cgacgtcgcc
    11881 gttatccagc ccgaatggat cgagcaggaa gccccgcacc ttgtccgcta ccattatttc
    11941 gagccgcact gggaacaaaa acgcggcgaa gtcgtcgcca gcgaacgcgt aacgctttac
    12001 ggcctggccg tattgccgcg ccgccccgtg tcttacggta aagttgcgcc cgaagaagca
    12061 cgtgaaatct ttatccgcag cgcgttggtg gcgcaggaat acgatttgaa agcggatttt
    12121 tttgtccaca acaaaaagct gattaaagaa attaccgaac tcgaacacaa atcgcgcaag
    12181 caagacgtat tggtcgatga cgaagccctg tttgcgtttt atcacgaacg gctgcccgat
    12241 ttttatatgg cggattcggt ttcagagggc ctgtgtcctg caaatccgca gcaaaccacc
    12301 ccctcccccg tgggggaagg ctggggagag ggcaaaacag ttgccgcaca aaccaacttt
    12361 tccgcaacct cagcaagccc tctccctaac cctctcccac aggagaggga acagagtgcc
    12421 tcagtttcaa cggtttcagg cagtctgaaa acaatgtctt gcgaagcaag gctgaatttc
    12481 tgcgaagcta aaactaaaac cgaaagcagc ctgcactccc aaaggctatc tgaaaactac
    12541 accccgccat tttcagacgg cctgcgtcct gcaaaccccc agcaaaccac cccctccccc
    12601 gtgggggagg gctggggaga gggcaaaaca gttgccgcac aaaccaactt ttccgcaacc
    12661 gcagcaaacc ctctcccaca ggagagggaa cagagtgcct cggctttaac agtttcagat
    12721 gaccccaaac ccaaaaagca gcctgcatct caaaaaggcc gtctgaaacc tttgcccctt
    12781 gccgatatcc gcacctttga agcctggctc aaaaccgccg agcgcgacaa cccgcgcctg
    12841 ttgttcctca gccgcgacga cctcatgcag cacgccgccg cgcacatcac cgaagaacaa
    12901 ttccccaaac actggcagac cgcagacggc aaattcaaac tcagctaccg cttcgagccg
    12961 caccacccgc tcgacggcgt taccctcacc ctgccgctca ccgtgctcaa ccgcatcagc
    13021 cccgccgccc tcgaatggct cgtgcccggc atgatacgcg aaaaaatcca gctacaaatc
    13081 aaagcactgc ccaaacaaat ccgccgcatc tgcgtacccg tacccgaatt catcacccaa
    13141 tttttaagcc aaaaccccga ccgcaacgcc cccatcctgc cccagctcgc ccaagccatc
    13201 gccaaaaccg caggcgacat ccgcatactc gagcaaatca accaagacga atgggccgcg
    13261 ttcaggctgc ccgaacactg ctatttcaac ctccgcatca tcgacgacgg cggacaagag
    13321 ttagccatgg gtcgcgattt aatccaaatc caacaacaac ttggcaaagc cgccaccacc
    13381 accttccgcg acaacaccca agaattcgag cgtgacaacg ttaccgcatg ggacatcggc
    13441 atcctgcccg aatccatcaa attcgcccgc ggcaaacaac agctcaccgg ctacctcggc
    13501 ctgcaaaaag aaaaagacgg ccgcatcgcc ctgcgcctgt tcgacacgtc tgccgccgcc
    13561 ggacacgccc acagattagg cgtgattgaa ctcatgaaac tacaactaaa agaacaagtt
    13621 aaagatctga acaaaggcat ccaaggcttc acccaagccg ccatgctgct caaacacatc
    13681 aacgccgaca ccctgcgcga cgacctcacc caagccgtct gcgaccgcgc ctttatcggc
    13741 gaagacaagc tgccgcgcaa cgaaaaagcc ttcaaagaac aaatcaaacg cgcccgcagc
    13801 cgcctgcccg ccgtcaaaga agccctcagc cgctacctgc aggaaaccgc agccgcctac
    13861 gccgaactga acggcaaact cggcaaacac ccattgaccc acctcctaag actacgcctg
    13921 caaaccctgc ttgcacccgg cttcgccacc cgcaccccgt gggcacaatg gccgcgcctc
    13981 cccatctacc tcaaagccat gaccctgcgc ctcgaaaaat acagcagcaa ccccgcccgc
    14041 gacgcagccc gcgaagccga tatccaagag ctggaacaaa tgtggcagga aaaaactgac
    14101 ggcttggcga aacaaggaca gcccgtttca gacggcctcg ccgcgtttaa atggatgatt
    14161 gaagaattga gagtgtcgct gttcgcgcag gagttgaaga cgccgtatcc ggtgtcggta
    14221 aagcggctgt tgaagatgtg
//
LOCUS       DBJGGI010000053        13722 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.053, whole genome shotgun sequence.
ACCESSION   DBJGGI010000053 DBJGGI010000000
VERSION     DBJGGI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13722
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.053"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(<1..410)
                     /locus_tag="ACSIGJ_001558"
     CDS             complement(<1..410)
                     /locus_tag="ACSIGJ_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HHQ1330391.1"
                     /translation="MKTGIQTELAQALLSHEKIWANEEKTILAKNILLDLVEKTDPSI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSINNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRN"
     gene            complement(628..1548)
                     /gene="truB"
                     /locus_tag="ACSIGJ_001559"
     CDS             complement(628..1548)
                     /gene="truB"
                     /locus_tag="ACSIGJ_001559"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HHQ1330392.1"
                     /translation="MTNKPAKRPVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHTG
                     VLDPLATGLLPVCFGEAAKFAQYLIDADKAYTATLKLGEASSTGDAEGEIIAAARADI
                     SLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKPRDITVYSIDIA
                     EFDAPKAVISVRCSKGTYIRTLSEGIAKHIGTFAHLTALRRTETAGFTIAQSHTLEAL
                     ANLNETERDGLLLPCDVLVSHFPQTVLNDYAVHMLQCGQRPRFEEDLPSDTPVRVYTE
                     NGRFVGLAEYQKEICRMKALRLMNTAASSA"
     gene            complement(1606..2130)
                     /locus_tag="ACSIGJ_001560"
     CDS             complement(1606..2130)
                     /locus_tag="ACSIGJ_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330393.1"
                     /translation="MNNLIPEHLAAYAHSDNLQIEGGHRCFSLSCQGRDTFHIRYYGE
                     PFDGLITDTDKAPVKIVAVEAVSGDEIVLFDGAEHGYNAMFCDKYSQNQKQNRTLTDL
                     DEYTYRVPIHLYYNIDYEDEYEDFVNSEGQVPLIDGRIISFDSLKRNGFDAISIDLID
                     EKHSVRELLNEELS"
     gene            complement(2141..2512)
                     /gene="rbfA"
                     /locus_tag="ACSIGJ_001561"
     CDS             complement(2141..2512)
                     /gene="rbfA"
                     /locus_tag="ACSIGJ_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HHQ1330394.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITINEVE
                     VTRDYSHATVFYTVLNQDTREITEEVLEHARGHLRSELSKRIKLFKIPELHFKYDESL
                     ERGMSLSALIDQVAAEKPVED"
     gene            2695..3939
                     /gene="clpX"
                     /locus_tag="ACSIGJ_001562"
     CDS             2695..3939
                     /gene="clpX"
                     /locus_tag="ACSIGJ_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HHQ1330395.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCLEILYEG
                     DNGGTPPENAGGEPEESGKLPTPAEIVANLDDYVIGQGQAKKALAVAVYNHYKRLRHP
                     KADGGVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQHGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTANILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENAGITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGIENVGLEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            complement(4046..5239)
                     /locus_tag="ACSIGJ_001563"
     CDS             complement(4046..5239)
                     /locus_tag="ACSIGJ_001563"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HHQ1330396.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVKHSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQAARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVRSQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCAL
                     DTPYRGRSSEIAATSLKHGVMILVAGADVLRFAPSLLLNDEDIAEGLRRLEHVLTEFA
                     AANRP"
     gene            complement(5338..5547)
                     /locus_tag="ACSIGJ_001564"
     CDS             complement(5338..5547)
                     /locus_tag="ACSIGJ_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330397.1"
                     /translation="MGWISSKRKKAPHVCVVPIAVSGCKRFALHDEMPSEDKNIGEIL
                     NQNAAAPQALYRNFFVFFSYPVENI"
     gene            5644..6144
                     /locus_tag="ACSIGJ_001565"
     CDS             5644..6144
                     /locus_tag="ACSIGJ_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330398.1"
                     /translation="MDAMPQTAVQNENLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANKRACRFAEKHRLFFTPAQAELSLNKHKNRRLLTIETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            6229..6783
                     /locus_tag="ACSIGJ_001566"
     CDS             6229..6783
                     /locus_tag="ACSIGJ_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330399.1"
                     /translation="MKKIIASALIATFALTACQDDTQARLERQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAQAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLIGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPVNYRRPAMRGFGRRR"
     gene            6797..6997
                     /locus_tag="ACSIGJ_001567"
     CDS             6797..6997
                     /locus_tag="ACSIGJ_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330400.1"
                     /translation="MPSEGLSDGIFVFVRGIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVIPPLGFQTASYRTVKP"
     gene            complement(7348..8736)
                     /locus_tag="ACSIGJ_001568"
     CDS             complement(7348..8736)
                     /locus_tag="ACSIGJ_001568"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HHQ1330401.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGCDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLAPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGTSFGYQTRALSKPVDLIVATPGRLTDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPADRQTLLFSATWDGAVGKLARKLTKDSEIIEVERVDGQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLTDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPARKRKSAGGKPKG
                     KGGWGDRKSGGRRGDHKPGKEGFGGKTRGEGFKKEGFKKDSFKKTGEGFKGKRKAGDS
                     FAGKSERRYKDR"
     gene            9064..10011
                     /locus_tag="ACSIGJ_001569"
     CDS             9064..10011
                     /locus_tag="ACSIGJ_001569"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHQ1330402.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPGEYEAWLAEVLEAVAFVTGFEPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVIAENGRKFWVNLDKYLDTGFFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDMERHKIVRADVFQYLQTAY
                     GEGRQFDLIVMDPPSFSNSKKMPGILDIQRDHQKLIDGAVNLLASDGILYFSNNLRSF
                     VLDDSVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            complement(10063..10395)
                     /gene="trxA"
                     /locus_tag="ACSIGJ_001570"
     CDS             complement(10063..10395)
                     /gene="trxA"
                     /locus_tag="ACSIGJ_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HHQ1330403.1"
                     /translation="MSSELIVHTGDAAFEQDVLKSDLPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            10693..11166
                     /gene="dnrN"
                     /locus_tag="ACSIGJ_001571"
     CDS             10693..11166
                     /gene="dnrN"
                     /locus_tag="ACSIGJ_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein DnrN"
                     /protein_id="HHQ1330404.1"
                     /translation="MTDFSVWEAAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLADMRDELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTGNFHAPEGACGSWTRLYALAKEMADDLNDHIHLENDILFARVLDS"
     gene            complement(11237..12058)
                     /gene="nadE"
                     /locus_tag="ACSIGJ_001572"
     CDS             complement(11237..12058)
                     /gene="nadE"
                     /locus_tag="ACSIGJ_001572"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HHQ1330405.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAAQANAKGFVVGVSGGI
                     DSAVVSTLAARTGRTTLLLDMPIRQHPGQLERARRHIRNLQGQYANVSAQTANLTDTF
                     QTFEQTVGVHRTAFANQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKVEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRRREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            12255..13610
                     /gene="xseA"
                     /locus_tag="ACSIGJ_001573"
     CDS             12255..13610
                     /gene="xseA"
                     /locus_tag="ACSIGJ_001573"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HHQ1330406.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAQPLKEGDHIEVAGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPVRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPAAVQGAGSGFQIAQAIKTASQRAECDVLIVCRGGGSIEDLRAFNE
                     EPVVRAIEACTIPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLVQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMTQNLRA
                     HTARFERQTQALQHCRPDVSVYRQDIVRLQTALPAAFSRLLARRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFSDGETDVRVSKEQGQQDLFDC
                     I"
BASE COUNT         3201 a         3629 c         3654 g         3238 t
ORIGIN      
        1 ttattgcgtt taaaataaat ttcttcgcct tcttcggtgc tttgtccgcc attcaatacg
       61 caatctttaa acggaaaatc caacacaata tccgaattgt ctttcaaaaa gcggttgccg
      121 tccgtcaaac caatgcggtt ggcgtatttt gtgtaggaat tattgatgct gtgtttgtcc
      181 aagaaaaaac ggaaatcctg caatttaaac accaaaacac cattcacttc cacaaagaaa
      241 tggcgtttta actcatcatt attcaataac aaaccgataa tggacgggtc ggttttttcc
      301 accaaatcca acaggatgtt tttggccaaa atggtttttt cttcgttcgc ccatattttt
      361 tcgtgtgata gcagggcttg ggctaattcg gtttgaatgc ctgtcttcat cgttataaat
      421 cctcatcagt tattttaatg tgccattctt gtgtaacggc aaatggcttt acaaataaat
      481 tataataaaa tcaattcttt gatgtctttg ctgtttccgg gggaagttcg caaagttgcg
      541 ataagcgggg cgggtgtccg agtgtgtcgt gattttacgg aaatatcaac acattattca
      601 aacgcagccc gtatttttaa ccgccgctca ggcggaggat gccgccgtgt tcatcaggcg
      661 caaggctttc atacggcata tttctttttg atattccgcc aaaccgacaa agcggccgtt
      721 ttccgtgtaa acgcgtaccg gcgtatcgga aggcaggtct tcctcgaaac gcggccgttg
      781 cccgcattgg agcatatgga cggcataatc gtttaaaacg gtttggggaa agtgtgaaac
      841 caatacgtcg cagggcagca gcagaccgtc gcgttctgtt tcgtttaaat ttgccaaagc
      901 ttcgagcgtg tggctttggg cgatggtaaa gccggcggtt tcggtgcggc gtagggcggt
      961 caggtgggcg aatgtgccga tgtgtttggc gatgccttcg ctgagggtgc ggatgtaggt
     1021 gcctttgctg caacgcacgc ttatcacggc tttgggcgcg tcaaactcgg caatatcgat
     1081 agagtaaacg gtaatgtcgc gcggtttgcg ttcgataaca atgcctttgc gcgcgtattc
     1141 gtacaacggt ttgccttcgt gcttgagggc ggaaaacatc ggcggcactt ggcggatgtt
     1201 gcctgtcagt gcttggcagg ccgtctgaaa ttcggctaag gaaatatcgg cgcgggcggc
     1261 ggcaatgatt tctccttcgg catcgcccgt gctgctggct tcgccgagtt tcagcgtggc
     1321 ggtgtaggct ttatcggcat caatcagata ttgggcgaac ttggccgctt cgccgaagca
     1381 gacgggcaaa agtccggttg ccaaaggatc gagcacgccg gtatgtccgg ctttttcggc
     1441 atggaacaaa cgccgcgctt tttgcagggc ggtgttgctg gaaaggcctt cgggtttgtc
     1501 gagaagaaga acgccgttga ccgggcgttt ggcgggttta ttggtcatat taattaggga
     1561 atattaatgt tcagaccgtc tgaaagggtt cggacggcct gcgtgtcagc ttaattcttc
     1621 attcaataat tcacgaacag agtgtttttc atcaattaga tcaatgctga ttgcatcaaa
     1681 gccatttcgt tttaatgagt caaaactaat gatgcggcca tcaattaagg ggacttgtcc
     1741 ttcagaattg acgaaatctt catattcatc ttcgtagtct atattgtaat aaagatgaat
     1801 cggaactcgg taagtatatt catccaaatc agttaacgtt ctgttttgct tttgattttg
     1861 gctatatttg tcgcagaaca tagcgttata gccatgttcc gccccatcaa acaatacgat
     1921 ttcatcgccg cttacagctt ctaccgccac aatttttacc ggcgccttat cagtatcggt
     1981 aatcaatcca tcaaaaggct ctccatagta acggatgtgg aaagtatctc taccttggca
     2041 ggataatgaa aagcaacgat gcccgccttc aatttgcagg ttatcactat gtgcataggc
     2101 ggctaaatgt tcgggaatca gattgttcat tctggcagta tcagtcttca accggttttt
     2161 ccgccgccac ttgatcgata agggcggaca ggctcatacc gcgttccaaa gattcgtcgt
     2221 atttgaaatg cagttcgggg attttgaaca gcttgatgcg tttggacaat tcgctgcgga
     2281 ggtgtccgcg cgcgtgttcc agcacttctt ccgtaatttc gcgcgtatct tggttcaaaa
     2341 cggtgtagaa cacggtggcg tggctgtaat cgcgggtaac ttcgacttcg ttgatagtga
     2401 tgaagccggc gcgcgggtct ttcagtccgg tacggacgag ttcggcaagc tcgcgcataa
     2461 tttgttcttt gacacggtct tggcgggcgt agccgcgttg gggttttctc atgaatatcc
     2521 tcagatttgg tttcagacgg catttctgcc gccggggcgg ttgcaaaggg gcgtattata
     2581 gccgaatcgg cgggatttta aggtaatacg ttgataagca cggatatagt tattatactt
     2641 ggcaacccga acggcgcatg ccgtttctgc aacgcacaca ttgtaaagaa agccatgtcc
     2701 aacgaaaacc gtacctgttc cttttgcgga aaatccaaat cacacgtcaa acatttgatt
     2761 gagggcgaaa acgcctttat ctgcgacgaa tgcgtctcaa actgcctcga aatattgtac
     2821 gaaggcgaca acggcggcac gcctccggaa aatgccggag gggagccgga agaatccggc
     2881 aaactgccca cgcccgccga aatcgttgcc aacctcgacg attatgtcat cgggcagggg
     2941 caggcgaaaa aggcgctggc ggttgcggtt tacaaccatt acaaacgcct gcgccacccg
     3001 aaagccgacg gcggtgtcga attgtcgaaa tccaacatcc tgcttatcgg cccgaccgga
     3061 tcgggtaaaa cgctgttggc gcaatctttg gcacgcaaac tggacgtgcc gttcgtgatg
     3121 gcggatgcga ccacgctgac cgaagccggc tatgtcggcg aagatgtcga acaaatcatt
     3181 accaaactgt tgggcaaatg cgatttcgat gtcgaaaaag cccagcacgg cattgtctat
     3241 attgacgaaa ttgacaaaat ttcgcgtaaa agcgacaacc cgtccatcac gcgcgacgtg
     3301 tccggcgaag gcgtgcagca agccttgctg aaactgattg aaggtacggt ggcaagcgtt
     3361 ccgccccaag gcggacgcaa gcatccgaat caggaattta tcaacgttga taccgccaac
     3421 atcctgttta tctgcggcgg cgcgtttgca ggcttggaaa aagtgattcg ccagcgcacc
     3481 gagaaaggtg gtatcggttt cggcgcgtcc gttcacagca aggacgaaaa tgccggcatt
     3541 accaagctgt tcggcatcgt cgaaccggaa gatttaatca aattcggcct gattccggaa
     3601 ttaatcggac gtttgcccgt gattgcaact ttagaagaac tggatgagga cgcgctgatt
     3661 aatattttaa ccgagccgaa aaacgctttg gtcaagcagt atcaagcctt gttcggcata
     3721 gaaaacgtcg ggttggaatt tgaagaaggc gcattgcgtt ccatcgcgcg gcaggcaatg
     3781 gaacgcaaaa ccggcgcgcg cggcctgcgt tccatcgtcg aacgctgcct gctcgatacg
     3841 atgtaccgcc tgcccgattt gcaaggcttg aaaaaagtgg tggtcggcaa ggcagtcatc
     3901 gaagagggca gggaaccgga attggtgttc gagtcctgat ggcaaacaaa accgcttcgg
     3961 aacgttggat cacgtttccg aagcggtttt ttgtttctga tgccgtctga ggccttcccg
     4021 tcttcagacg gcatctgctt tctgcctagg ggcggtttgc ggcggcaaat tccgtcagca
     4081 cgtgttccaa acgccgcaaa ccttccgcaa tatcctcatc gttcaacagc agcgaaggcg
     4141 cgaaacgcaa tacgtccgca cccgcaacta agatcatcac gccgtgtttc aaggaggtag
     4201 cggcgatttc ggatgagcgt ccgcgataag gcgtgtccaa cgcgcagccg agcagcagcc
     4261 ccatcccgcg aacctgtgag aacaagcctg ttttcctgcc caaatccagc aatgccgtct
     4321 gaagtttctg cccctgactg cggacgttgt gcaaggtttc cggcgcgttg atgatgtcga
     4381 aggcgcggct gccgaccgca cacgccatcg ggttgccgcc gaaagtcgag ccgtgcgtgc
     4441 ccggttggaa ggcggcggca atcttttctg tcgccagcat cgcgccgatc ggaaagccgc
     4501 agcccaaggc ttttgccgaa ctcaaaatat cgggaacaac gccgtaatgt tcataggcaa
     4561 acagcctgcc cgtatgcccc atcccggttt gaacttcgtc caaaatcaac aacgcattgt
     4621 gccggtcgca cagacggcgc gccgcctgca aatattccgc agtggcgggc aggatgccgc
     4681 tttcgccctg tatcggctcg atgatgaccg cgcaggtctg ttcgccgacg gcagcttcca
     4741 gcgcggcaat atcgttgaac ggaacgtgcg taatgccttg cggcagcggt gcataatcct
     4801 tactgtattt cggctgtccg ccgacggaca cggtaaacag cgtgcgtccg tggaaactgt
     4861 tgatacaggc gacgatttcg cttttgcctc cgccgaaacg gtcgcgcgca tatttccttg
     4921 ccagctttaa cgccgcctca ttcgcttccg cgcccgagtt gcagaaaaaa accttgtcgg
     4981 caaaactgtg tttcaccaat ttttgcgcca attcctgcgc cggccgcgtc gtatagatat
     5041 tggaaatgtg ccacagcttg tgcatctgcg cgtttaaagc atcgacaagg gcagggtggc
     5101 agtgccccag agcgttaacg gcgatgccgc ctgaaaaatc aatatattca cgcccttccg
     5161 tatcccaaac gcggctgcct gaagcgcgtt cgggaatcat cggggcaaag gcgaaattgg
     5221 gggtcagata gttttgcata attgctcctg attgcggttt tgttcgggat tatgcaccgt
     5281 ttttcaaggc ggataaaggg aaattgccga caatatcggg cagtggtaaa aataaaccta
     5341 aatattttca accggatagc tgaaaaaaac aaaaaaattt cgatataatg cttgaggcgc
     5401 ggcagcgttt tgatttagaa tctccccaat atttttatct tcggacggca tttcatcatg
     5461 caaggcaaaa cgtttacaac cgcttactgc tattggtacc acacagacgt gcggtgcttt
     5521 tttgcgcttg cttgaaatcc atcccatacg caaaacaggc cgctcgaaaa cggggcggct
     5581 tttttgttac cttcacacag gtcgcccgat tcggcaacac acgacagaat caggaaaatg
     5641 accatggatg caatgcccca aaccgccgta caaaacgaaa acctgcacac ttgcagcatt
     5701 attgtcgatg atgcgcagga tttttccccc atgcttctcg atgcagggcg ggacgacacg
     5761 ctcatcagcg aatcgatgat tccgcaaatc cgcaccgttg ccgccctgat agccgccgaa
     5821 cggcacgact tcagccgcag cagccccgcc gagtttaccg atgccgccga tttttttgcc
     5881 gcacggattc tcgtattggg cgtgcgacgc ttccatctgg acgtatcgct gcttcagatt
     5941 ttgaaaaccg caaacaagcg cgcctgccgc tttgccgaaa aacaccgcct gtttttcaca
     6001 cccgcacaag ccgaattgag cctgaacaaa cacaaaaacc gccgcctgct gaccatagaa
     6061 accgaacacg aagtcgaaaa caaaggcaac cctgtcgcca acagcctcgc atttgtccgc
     6121 aaactgcaca ccctacccct gtaaacatca cgatttgccg gcagaatgct tgccaaaatt
     6181 cccgacggcg atacaataca accgattttc cacccgaaca ggacacacat gaaaaaaatc
     6241 atcgcctccg cgcttatcgc aacattcgca ctcaccgcct gccaagacga cacgcaggcg
     6301 cggctcgaac ggcagcagaa acagattgaa gccctgcaac agcagctcgc acagcaggca
     6361 gacgatacgg tttaccaact gactcccgaa gcagtcaaag acaccattcc tgcccaggcg
     6421 caggcaaacg gcaacaacgg tcagcccgtt accggcaaag acgggcagca gtatatttac
     6481 gaccaatcga caggaagctg gctgctgcaa agcctgattg gcgcggcggc aggcgcgttt
     6541 atcggcaacg cgctggcaaa caaattcaca cgggcgggca accaagacag ccccgtcgcc
     6601 cgtcgcgcgc gtgctgccta ccatcagtcc gcacgcccca atgcgcgcac cagcagggat
     6661 ttgaacacgc gcagcctccg tgcaaaacaa caggcggcgc aggcgcagcg ttaccgcccg
     6721 acaacgcgcc cgcccgtcaa ttaccgccgt cccgctatgc gcggtttcgg cagaaggcgg
     6781 taaacccggc gcgtcaatgc cgtctgaagg gctttcagac ggcatttttg tatttgttag
     6841 gggcattgtt atgttgccgt ttgattttca gacggcattt tgtttccaag cgtttgatgt
     6901 cgggatggca attctgatct acgtgcgttg gcagggcggc attgtcatac cgccgttggg
     6961 ttttcagacg gcatcatatc gaaccgtcaa gccgtagggc gagattgggt aggcaggaat
     7021 gtccatcctg catctgaaac ccttctgcgt cattcccatg caggaaggaa tctagatatt
     7081 tgaatttacg gaaattttca atcgttgcca aagcggaaaa gggtttttat agtggattaa
     7141 caaaaatcag gacaaggcga cgaagccgca gacagtacag atagtacggc aaggcgaggc
     7201 aacgcggtac cggtttaaat ttaatccact atattgcaga ttaatccttt gggtatcaaa
     7261 gccgggcggt gctgaaacat tacaaaaatg ccgtctgaaa ctcgaaatcg gtttcagacg
     7321 gcattggttt acgcggcagg ttggggctta gcggtctttg taacggcgtt cgcttttgcc
     7381 tgcaaaagag tcgccggctt tgcgtttgcc tttgaagcct tcgccggttt ttttgaaact
     7441 gtctttctta aagccttctt tcttgaaacc ttcgccgcgc gttttgccgc cgaagccttc
     7501 tttgcccggt ttatgatcgc cgcgccggcc gccggatttc ctatcgcccc agccgccttt
     7561 gcctttcggc ttgccgcctg cggatttgcg tttgcgggcc ggctccatgc cttcgatggt
     7621 cagttcgggc agtttgcggt taatgtattt ttcgattttg tggactttga cgtattcgtt
     7681 cacttcggca aacgtaatcg caatacccgt gcggcctgcg cggccggtgc gcccgatgcg
     7741 gtggacgtag tcttccgcct gtttcggcag gtcgtagttt atgacgtggg taatggtcgg
     7801 tacgtcaata ccgcgtgcgg caacgtcggt ggcaaccaaa attttgcagc ggcctttacg
     7861 caaatccgtc agcgtgcggt tgcgccagcc ctgcggcata tcgccgtgca ggcagttggc
     7921 ggcgaaacct ttttcgtaca attcatccgc gatgacttcg gtcatcgctt tggtggacgt
     7981 gaaaatcaca cattggtcga tgttggcatc gcgcaggatg tggtcgagca ggcggttttt
     8041 gtggcgcata tcgtcgcagt acaacaactg ctcttcgatt ttgccttggc cgtccacgcg
     8101 ttcgacttcg ataatttcag agtctttggt cagtttgcgc gccagtttgc cgactgcgcc
     8161 gtcccaagtg gcggagaaca ataaagtctg acggtcggcc ggggtggctt ccacgatggt
     8221 ttcgatgtcg tcgataaagc ccatatccaa catacggtcg gcttcgtcca aaatcagcac
     8281 ttccaaacgt tcaaaatcaa ctttgccgct ttgcatcagg tccgtcagac ggcccggagt
     8341 ggcgacaatc aaatcgaccg gtttgctcag ggcgcgggtt tggtagccga acgatgtgcc
     8401 gccgacgatg ctgacggtgc ggaaccagcg catatttttg gcatacgcca gcgcgttttt
     8461 ctcgacttgc gccgccagtt cgcgggtcgg tgccaacacc agcgcgcgcg ggcctttgcc
     8521 cggtttttcg ctgcgtttgg tcagtcgctg caaagtcggt aacagaaagg ctgcggtttt
     8581 tccggagccg gtttgcgccg aagccatgat gtcgcagcct tccaaagcaa acggaatcgc
     8641 ctgcgcctga atcggcgtcg ggctttcgta accctcgctg ctgacggcgg acaaaatgtt
     8701 tttatcaagg ttcaaatcgg caaatttaat agacatggct atccttggaa gacaacaacg
     8761 cccgcgtccg aaaggttcag acggcctgaa gcagggaagg gtaacgatac ggggaatgtg
     8821 aagttacagg gttgtcgcaa gcggcttgct tgtggtttac atcgacggca acctgcacag
     8881 atatgctttg caaaatcaaa gcaacagaag aaacgcgccc gtaagggcgg tttaggttgg
     8941 aaggcgatgg cttcgtatga caagataagc gacggatggt atagggtttg cccgaaaagg
     9001 tcaagcaatc gggcgttaat tacagtaaaa tttccgtttt ccactttatt ccgcctgaaa
     9061 tcaatgaccg acatcacacc gtttgccaac cgcttgggca aaaacatcaa acaccttatg
     9121 aaatgggcga aacgcaacgg catcgaagcc tggcgcattt acgaccgcga catcccccaa
     9181 ttcccttttg ccgccgatgt ttacggcgat cgaatccacc ttcaggaata cgataccggc
     9241 tggctgatgc ggcccggaga atacgaagcg tggcttgccg aagtattgga agccgtcgct
     9301 ttcgttaccg gttttgaacc cgaacaaatc cgtctcaaac gccgcgaacg ccaaaaaggt
     9361 ttgcagcagt acgagaaaac cggcaaggcg ggcgacgatt tcgtcattgc cgaaaacggc
     9421 cgcaagtttt gggtcaacct cgacaaatac ctcgacaccg gcttcttcct cgatcaccgc
     9481 aacacgcgca aaaaagtcgg cgaaacggcg gcgggcaaac gttttttgaa cctgttttcc
     9541 tacaccggca gcttcaccgt ctatgccgcc accggcggcg cggcatccag cgaaaccgtc
     9601 gatttgtcca acacttatct cgattgggcg aggcgcaatt ttgaattgaa cggcatcgat
     9661 atggaacggc acaaaatcgt ccgcgccgac gtgttccaat accttcagac ggcatatggg
     9721 gaaggcaggc agttcgacct cattgtgatg gacccgccca gtttttccaa cagcaagaaa
     9781 atgcccggca tcctcgacat ccagcgcgac catcaaaaac tcatagacgg cgcggtgaac
     9841 ctgctcgctt cagacggcat tttgtatttt tccaacaacc tgcgcagctt cgttttggac
     9901 gattcggtat cggaacaata cgccgtgaaa gacatttcca aacaatccgt tcccgaagat
     9961 ttccgcaaca aaaaaatcca ccggtgttgg gaaatccgcc acaaatctta agggtgcgaa
    10021 aaagccgccg gaatcttccg gcggcttttc gattgcgctt tatcaggcga tggaggcttc
    10081 gacaaaggcg gtcagctgac cttttgccaa tgcgccgact ttggtggcga cgacttcgcc
    10141 gtttttgaac accatcaggg tcggaatgcc gcgcacgccg aaacgggacg gggtggcttc
    10201 gttgtcgtcg atgttgattt tgactacttt cagacggcct tcaaattcgg cggcaatgtc
    10261 gtccaaaatc ggggcaatca ttttgcaggg gccgcaccac ggagcccaaa agtccagcag
    10321 gacggggagg tcggatttca aaacatcttg ctcgaaggcg gcatcgccgg tgtgtacgat
    10381 taattcgctg ctcattattt ttccttttcg gttgggattc gcacgatagc gtgcaggata
    10441 gggcttgggc ataaaaattt caagtgggcg ggcgggataa tagtttttct gtaccgcccc
    10501 aatcgccttt gcaaattcta aaaaacggtt cggacagtat tttatttttg aaaattactt
    10561 ttattatcaa agtataagga atgtttttga ggctgtacag aaacgcgcgg gcggtgtatg
    10621 ctccgtttaa atagattctt aaagtatgaa tgttcaaaac caaacccgaa accctataaa
    10681 aggaacactg caatgactga tttttccgtt tgggaagctg ctccctttgg cgcaaccgtt
    10741 gatcatatcc tgcaacgcta ccacaatgtc caccgcgcac aatttgaaga attggtgccg
    10801 ctggcgcaga aagtggccca agtccatgcc gatacctttc cggcggaaat tgccgggctg
    10861 cttgccgata tgcgggacga gctgctgatg catatgatga aggaagagcg gatgctgttt
    10921 ccgatgatta atcagggtgt cggacgcggt gcggcgatgc ccatcagcgt gatgatgcac
    10981 gaacatgaag aacacgaccg cgccatcgca cggctcaaag agctgaccgg taatttccac
    11041 gcacccgaag gggcttgcgg aagttggacg cgcctttacg cgctggcaaa agaaatggcg
    11101 gacgatttga acgaccacat tcatttggaa aacgatattc tgtttgcccg cgtactcgat
    11161 tcttaaggga taggagtcgg atatttcaat gccgtctgaa atacggtttt tccgtttcag
    11221 acggcatttt catgtttcag cccagcaatt cgggcggaat gcggcacaca ggaatcgggt
    11281 tgattttgtg ctgcatcgcg cggtgaagcc gcgtatagat ttccagaact tcgcgccgcc
    11341 gtccttcaaa atcttcgggt ttgcgcgtgc cgtacacgcc cattgcccac tccagttcgg
    11401 gatagcttgc gcccatctgt tcctcgtcgg tgcgttccgt atcccacagg ccgtcggtcg
    11461 gcggggcttt ttgaatcgcc tcgtccacgc ccaatgcttc ggcaagccgg taaacctgcg
    11521 ttttggtcag gtcggcaatc gggctgatgt ccacgccgcc gtcgccgtat ttggtaaaaa
    11581 aacccacgcc gaaatcttca accttattac ctgtccccgt aaccagcagt ccgtgtatct
    11641 gcccgtagta gtacagggtc agcatacgca ggcggcttct ggcgttggcg agcgaaagcg
    11701 gctgattggc aaatgccgtc cgatgaacgc cgacggtttg ctcaaaggtt tggaaggtgt
    11761 cggtcagatt ggccgtttgc gcgcttacat tggcatattg cccttgcaga ttgcggatgt
    11821 gccgccttgc ccgctcgagc tggccggggt gttggcgtat cggcatatcc agaagcagcg
    11881 tggtgcggcc ggtgcgggcg gcgagcgtgg agacgacggc ggaatcgatg ccgccggaaa
    11941 cgcctacgac gaaccctttt gcatttgcct gagcggcgta ttcgtccaac catcggacaa
    12001 tatgtgtgat aactgcctgc gtatccattt ttcctcctgt cgaaaatatc cgcatcatag
    12061 cctgccggcg gtgcaatttg aatagattga gggcgggttg atgaatattt ttcccattcc
    12121 ccgcttaaaa aagtcatata ggaaaaggct ggcggcaaga tggcgcgtgt tataatattg
    12181 ttgacaattt agacggcacg atgccgcctt gggcttcaaa acatattcat attttttatt
    12241 atttgattga ttcgatgtct gatttttttc attctgacgt gttgtccgtt tccgagttga
    12301 acgcatttgc caaaagtatt ttggaaaacc accttgccgg cctgtggatt gcgggcgaag
    12361 tgtccaatct gacccgcgcc gccagcgggc attattattt ctcactcaaa gacagccgcg
    12421 cgcaggtgcg ttgcgcgatg ttcaagggtg cggcggcgcg tttggcgcaa cctttgaaag
    12481 aaggcgacca tatcgaagtg gcgggcaaaa tcagtattta cgaggcgcgg ggcgaatttc
    12541 agattaccgt aaacgaagta cggctcaaag gtttggggca gctttacgaa gcctacgagc
    12601 ggttgaaggc gcagttgcag gcggaaggcg cgtttgcggc ggaacgcaaa aaacctttgc
    12661 ccgtccgtcc gcaatgtatc ggcatcgtta ccagtctggc ggcggcggct ttgcgcgatg
    12721 tcgtaaccac cttgaaacgc cgcgcgcccg aaatccccgt catcgtttat ccggccgccg
    12781 ttcagggcgc gggcagcggg tttcagattg cccaagcgat taaaaccgca tcgcaacgcg
    12841 ccgaatgcga tgtgctgatt gtctgtcgcg gcggcggcag catcgaagac ttgcgggcgt
    12901 ttaacgaaga accggtcgtg cgcgccatcg aagcctgcac gattcccgtc gtcagcggcg
    12961 tgggacacga aaccgatttc acgctcgccg atttcgttgc cgacgtgcgc gcgcccacgc
    13021 cgaccggcgc ggcggaactg gtcagcccca accgccaaga atcactgcac cgcctcgtcc
    13081 aagcccaagg ccgtctgaaa accgttttgg agcaacgcta tttcgatgcc agccaaaaac
    13141 tcgactggct cgcgcggcaa atccgccacc cgcgccaaaa acttgacgag cagcgcgctt
    13201 caatcggcaa gctggcgcaa acgctgtctt actcgatgac gcaaaacctc cgcgcccaca
    13261 ccgcccgttt tgaacgccaa acccaagccc tgcaacattg ccgtcctgat gtttccgttt
    13321 accggcaaga tatcgtccgc cttcagacgg cattgcccgc cgccttcagc cggctgctcg
    13381 cccgtcgccg tcaaagcctg accgcccaag ccgcattgct cgaagccgtt tcgccgcagc
    13441 acattctgga acgcggcttc tccgttgtca aaaatacacg cggacaagtc atccgtaacg
    13501 ccgatgtttt gaagcagggg cagaagctgc acattacctt ctccgacggc gaaaccgacg
    13561 tgcgcgtaag caaagaacaa gggcagcagg atttgttcga ttgcatttaa acccaatcgg
    13621 aaaaaccgct ccccctccgc cgtcattccc gcgaaaacgg gaatccagtc cctaaaaagc
    13681 gggaatccgg aagcggaaag ccgcaggaat ttgtcggaaa aa
//
LOCUS       DBJGGI010000054        13631 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.054, whole genome shotgun sequence.
ACCESSION   DBJGGI010000054 DBJGGI010000000
VERSION     DBJGGI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13631
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.054"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..1707
                     /locus_tag="ACSIGJ_001574"
     CDS             <1..1707
                     /locus_tag="ACSIGJ_001574"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HHQ1330407.1"
                     /translation="YTKLTRKRQEIFFNQTLAFDEIDRLFDAKAFSKFSRHTADGKQP
                     VGEIKRRSDGTPAENLIIKGNNLIALHSLAKQFKGKVKLIYIDPPYNTETDSFAYNDK
                     FSHSTWLTFMKNRLEIAKELLKDDGLIFVQCDDKEQAYLKVLLDETFTRENFINCIAV
                     KMSEPSGNKMAHTSHRLPKIKEYILIYKNKNIKLNPIREQKSEWDNEYNIFLENFTQE
                     DKKFIDLIVNSQTENKEINGNTLKEIDILLKKISPISVNQKLAQLNIKDNEVIKWKLD
                     NAYRIVRTAASSSVKKLADEKKEICQQQFFSVISKRDKLLYIVKSDYSKDAKAPRVQV
                     LFAEDYLSISLCDLWTNINTTGLEAEGNVELKNGKKPESLIETIIKLATNENDIVLDY
                     HLGSGTTAAVAHKMNRQYIGIEQMDYIETLAVERMKKVIDGEQGGISKAVNWQGGGEF
                     VYAELSPFNETAKQQILACENSDGIKTLFEGLCERHFLKYNVSVNEFSQIIEEPEFQS
                     LALDEQKQMMLEMLDLNQMYISLSEMDDEQFAGCLNDDDKALSRAFYQSVKHQAEKKD
                     GE"
     gene            1697..4471
                     /locus_tag="ACSIGJ_001575"
     CDS             1697..4471
                     /locus_tag="ACSIGJ_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HHQ1330408.1"
                     /translation="MANNKTLFDWVEDRKSMLEEMEQTDFFALPEFVSNNLKYPFFEW
                     QKSALENFVIFDRTSKLKDFPDIKNRPTHLLFNMATGAGKTMMIAALILYYFEKGYRH
                     FLFFVNKNNIVYKTENNFIDPTHPKFLFTEKILQGDTVIPIRKVETFSQYSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEKEMNE
                     RTSDAEFERKGWEHMVLELLLNKNGNPGQNVLLEFTATLPENTEVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSGTLNKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYSAFLNWVENVQADDFSFLTTFSTSLNDSNNANEQGKTRTEQALKFMQENK
                     FEFVHLANWIKQNYQKHNVIITNSETNKNKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYITELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNKDFRELLIWANKKIPNPNAKSNNADSLQANPQTLSFQVHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFDRLQSKLD
                     IQNRNELQNKLLKDWQIEFLGLEQDKQVRPDDKLAGCLKILEMVEKHLNESDIPFIGT
                     KEFTPKKLWEIFGTPKQKWVKKDDIKTAIATQNDWYVMDNFAGTGLEEALIQFISARL
                     GDLKSQYDVHLIRNEEVFKLNNFADGEGFMPDFVLLLKDKQKSSSDSVDDFLHYQIFI
                     EPKGGHLVENDSWKEAFLKSITVEYGRDKILQKNTPHYRLIGLPFFTDHQKNGQFTEL
                     FPLGAASLEK"
     gene            complement(4620..5792)
                     /locus_tag="ACSIGJ_001576"
     CDS             complement(4620..5792)
                     /locus_tag="ACSIGJ_001576"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HHQ1330409.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGGDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTAEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALV
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDVSMAFTGHRDIQDVDASILRSKDWGRETV"
     gene            complement(5913..6155)
                     /locus_tag="ACSIGJ_001577"
     CDS             complement(5913..6155)
                     /locus_tag="ACSIGJ_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330410.1"
                     /translation="MVSLMVVVARVGFFIVGGIVQYLSVSVKYGGAVADCLFKWRYSL
                     ILLYLFGFLNQLVMFFEERRLGRFAGKRFQNDLIYF"
     gene            6142..6588
                     /gene="iscR"
                     /locus_tag="ACSIGJ_001578"
     CDS             6142..6588
                     /gene="iscR"
                     /locus_tag="ACSIGJ_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HHQ1330411.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPPAQINIAQIISAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            6617..7831
                     /locus_tag="ACSIGJ_001579"
     CDS             6617..7831
                     /locus_tag="ACSIGJ_001579"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HHQ1330412.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WTAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISIMWVNNEIGVV
                     QNIPAIGEICRERKIAFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELAQDTAHYLKLRDI
                     FLKGIEGIEEVYINGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            7816..8127
                     /locus_tag="ACSIGJ_001580"
     CDS             7816..8127
                     /locus_tag="ACSIGJ_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330413.1"
                     /translation="MGGALRLMSNKGCLRKNTMKVKIINLPNGYKRIIYGKYFEQFDL
                     DYEQDLDVLKKDIEFALSVIEYNRSIFKKFSSLFENKIIFVYQGGHHLDIIDRDKGSL
                     K"
     gene            8460..8927
                     /locus_tag="ACSIGJ_001581"
     CDS             8460..8927
                     /locus_tag="ACSIGJ_001581"
                     /inference="COORDINATES: protein motif:HMM:NF014691.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HHQ1330414.1"
                     /translation="MAHLQTIHRELFGNVYDWAGKIRRVDISKGNTRFANFAFIENES
                     RKLLEKLKNENYLRVLDKDKFAERAAYYLDELNVLHPFREGNGRTLRLFMTQLAIKNG
                     FQIHWQNISAEQMIQACIQAYHADSSLLARLIIRQFRTIVFFRRLKHQQTTIF"
     gene            8940..9326
                     /gene="iscU"
                     /locus_tag="ACSIGJ_001582"
     CDS             8940..9326
                     /gene="iscU"
                     /locus_tag="ACSIGJ_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HHQ1330415.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            9413..9733
                     /gene="iscA"
                     /locus_tag="ACSIGJ_001583"
     CDS             9413..9733
                     /gene="iscA"
                     /locus_tag="ACSIGJ_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HHQ1330416.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEANGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEDLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(9693..9914)
                     /locus_tag="ACSIGJ_001584"
     CDS             complement(9693..9914)
                     /locus_tag="ACSIGJ_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HHQ1330417.1"
                     /translation="MGGKAQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKRRDGFDTVPPFLCLKRGSSRRSRRSL"
     gene            9996..10496
                     /gene="hscB"
                     /locus_tag="ACSIGJ_001585"
     CDS             9996..10496
                     /gene="hscB"
                     /locus_tag="ACSIGJ_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001671 - ATPase activator activity
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HHQ1330418.1"
                     /translation="MSQYFTLFRIEPAFDIGTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTSFAPDFLMQQMEWRET
                     LMEARAGNNLESLKNLDNEIRAEQEKLFCGLKQSFARQDCDTAAQQVRQGRFLDKLRH
                     EISSAL"
     gene            10594..13344
                     /gene="gyrA"
                     /locus_tag="ACSIGJ_001586"
     CDS             10594..13344
                     /gene="gyrA"
                     /locus_tag="ACSIGJ_001586"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HHQ1330419.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDSAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLTDTINACLRLLDEPKTEIDELID
                     IIQAPDFPTGATIYGLGGVREGYKTGRGRVVMRGKTHIEPIGKNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKEKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKKQGYYLSEIQADAILRMSLRNLTGLDQKEIIESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     DLEDETADNENTLPSGKNGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGSIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSGASVISNVTEPEA
                     EN"
     gene            complement(13563..>13631)
                     /locus_tag="ACSIGJ_001587"
     CDS             complement(13563..>13631)
                     /locus_tag="ACSIGJ_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HHQ1330420.1"
                     /translation="EFRFNFGTPSRQLKILRDRCGI"
BASE COUNT         4234 a         3330 c         2932 g         3135 t
ORIGIN      
        1 tacaccaaat taaccagaaa aagacaagaa atctttttta atcaaacgct tgcttttgat
       61 gaaattgacc ggctttttga cgcaaaagca ttctcaaaat tctcccgcca caccgcagac
      121 ggcaaacaac ccgttggcga aatcaaacgc cgttcagacg gtacgccagc cgaaaatctt
      181 attatcaaag gcaataatct gattgccctg cattcgcttg ccaagcagtt taaaggcaaa
      241 gtaaagttga tttatattga tccaccgtat aatacagaaa ccgatagctt tgcatataac
      301 gataaatttt cacattccac ttggctaaca tttatgaaaa atcgcttgga aattgcgaaa
      361 gagttattaa aagatgatgg gctaatcttt gttcaatgcg atgataagga acaggcttat
      421 ttaaaagttt tattagatga aacttttaca agagaaaatt ttataaactg cattgctgta
      481 aaaatgtcag agccatcagg taacaaaatg gcacatacat cgcatagact accaaagata
      541 aaggaatata ttcttatcta taagaataaa aatataaaat taaaccctat tagagagcaa
      601 aaatcagaat gggacaatga gtacaatatt tttttagaaa attttacaca agaagataaa
      661 aaatttattg atttaatagt aaatagccaa acagaaaata aagaaataaa tggtaatact
      721 ttaaaagaaa ttgatattct tttaaagaaa atatctccta tttcagttaa tcaaaaatta
      781 gcccaattaa atattaaaga taatgaagtt ataaaatgga aactagataa tgcgtataga
      841 attgtaagaa cagcagctag tagtagcgta aaaaaattag cagatgaaaa aaaagaaatt
      901 tgccaacaac aatttttttc agttatttct aaacgagata aattattgta tatcgtcaag
      961 tcagattact caaaagatgc aaaagcacca cgagtgcagg ttttgtttgc agaagattat
     1021 ttgagtatta gtttatgcga tttatggaca aatataaata ccacaggact agaagcagaa
     1081 ggaaatgtag agttaaaaaa tggtaaaaag ccagaaagtt taattgaaac catcattaaa
     1141 ctagcaacca acgaaaacga catcgttcta gactaccact tgggcagtgg cacaaccgcc
     1201 gccgtcgccc ataaaatgaa ccgccaatac atcggcattg aacaaatgga ttatattgaa
     1261 acgcttgccg ttgaacgcat gaaaaaagta attgacggcg agcaaggcgg tatctcaaaa
     1321 gctgtgaatt ggcaaggtgg cggcgagttt gtttatgccg aactttcccc atttaacgaa
     1381 accgcaaaac aacaaatttt ggcgtgcgaa aattcagacg gcatcaaaac actgtttgaa
     1441 ggtttatgcg aacgccattt cttgaaatac aacgtcagcg taaatgaatt tagccaaatt
     1501 attgaagagc ctgaatttca atccttggca ttagacgaac aaaaacaaat gatgcttgaa
     1561 atgttggatt taaatcaaat gtatatttca ttatccgaaa tggacgatga acaatttgca
     1621 ggctgcctga atgatgatga taaagcgtta agccgtgcgt tctatcaatc agtaaaacat
     1681 caagcggaga aaaaagatgg cgaataataa aacgttgttt gattgggtgg aagaccgcaa
     1741 atcaatgctg gaagaaatgg aacagacgga ttttttcgcg ctgcctgaat ttgtttccaa
     1801 taatttaaaa tatccgtttt ttgaatggca aaaatcggct ttagaaaatt ttgtgatttt
     1861 tgaccgcact tcaaaattaa aggatttccc cgacatcaaa aaccgcccta cccatttgct
     1921 gttcaatatg gcaacaggtg caggcaaaac gatgatgata gcggcattga ttctgtatta
     1981 ttttgagaaa gggtatcggc attttttatt tttcgtcaat aaaaacaata tagtatataa
     2041 aacggaaaat aattttatcg acccgacgca tccaaaattt ttatttaccg agaaaatttt
     2101 gcaaggtgat acggtaattc ccattcgcaa agtggagaca tttagccaat attcagacgg
     2161 cattgaaatt aaatttacca gcattcaaaa actatataac gatattcaca ccgagcggga
     2221 aaatcaaacc acattggcgg atttgcacga attaaatctt gtgatgctgg gcgatgaagc
     2281 acaccattta aacgcgcaaa ccaaaggcaa aaaacaaggc gaattagatt tagaaaagga
     2341 aatgaacgag cgtaccagcg atgccgaatt tgaacgcaaa ggctgggagc atatggtttt
     2401 ggagttgtta ctcaataaaa atggcaatcc cggccaaaat gtgctgttgg aatttaccgc
     2461 cacgctgcct gaaaatactg aagtgcaaca aaaatatgct gataaaatca tcacaaaatt
     2521 tggtttaaaa gaatttttgc aaaaaggtta taccaaagaa atcaatttgg tatccggtac
     2581 gctgaataag aaagagcggg tgttgcacgc tttattgttt gcctggtacc gccatcaaat
     2641 cgcgttgaaa tacggcattg ccaatttcaa gcctgtgatg ttgtttagaa gtaagacgat
     2701 tgatgaatca aaagcggatt attcggcgtt tttaaattgg gtagaaaatg tgcaagcgga
     2761 tgatttttcg tttttaacta cattttcaac aagcttaaac gatagcaata acgccaacga
     2821 acaaggcaaa acccgtaccg aacaagccct gaaatttatg caggaaaata aatttgaatt
     2881 tgtgcatttg gcaaattgga ttaaacaaaa ttatcaaaaa cacaatgtca ttattaccaa
     2941 ctccgaaacc aacaaaaaca aaactgaaaa aaccgacagc gaaaccgaaa aattgctgaa
     3001 caatctggaa gcggctgata atccgatccg tgccattttt acggtggata gattaaccga
     3061 aggttgggac gttttaaatt tgtttgatat tgtgcgtttg tatgaagggc aaaacggtgg
     3121 cggttcgaat aaaaaatcag gcaaaacggc tgccgccacc gtatcggaaa agcagttgat
     3181 tggtcgcggc gtgcgttatt ttccatttgc gtttgaaggt aaacagccga ataaacgcaa
     3241 atttgacaac gatatgcaac atgaattgcg tatcttggaa gaattgtttt attacacgca
     3301 cgatgagcaa tcccgctata ttacagaact gaaaaacgag ttacgaaaag acggttattt
     3361 gcctgaaaaa gacgatgata aggtattggc aacatttaag ctcaaatctg aatttgcaga
     3421 taataaggat tttagagagt tgttaatttg ggcgaataaa aaaatcccca acccaaacgc
     3481 caaaagcaat aatgcagaca gcctgcaagc caatccgcaa acgctttcat tccaagttca
     3541 cggtaatcaa ctgttgcagg aaacgcaatt tacagccgat gaaaatgatg aaacagcccg
     3601 acaaatcggc acacaaaata attttactca aaccataaaa atgagtgaaa tggagcggca
     3661 tattttcaat aaagcattac atatcaaagg gaaaaatagt caatctttat tccattttga
     3721 ccgcctgcaa agcaaactcg acattcaaaa tcgcaatgaa ttgcaaaata aattgttgaa
     3781 agactggcag attgaatttt tgggattaga gcaagataaa caggttcgcc cggatgataa
     3841 acttgcaggc tgcctgaaaa tcttggaaat ggttgaaaaa catctgaatg aaagtgatat
     3901 accgtttatc ggtacaaaag aatttacacc taaaaaattg tgggaaattt tcggcacgcc
     3961 aaaacaaaaa tgggtcaaaa aagatgatat aaaaactgct attgccacgc aaaatgattg
     4021 gtatgtgatg gataattttg ccggaacggg tttggaagaa gcgttaattc aatttatttc
     4081 agcgcgcttg ggcgatttga aatctcaata tgacgttcat ttaatccgca atgaagaagt
     4141 gtttaaattg aataactttg ccgatggcga aggatttatg ccggactttg ttttattgct
     4201 gaaagataaa caaaaatctt cttccgacag cgtggacgac tttttgcatt accaaatttt
     4261 cattgaaccg aaaggcgggc atttagtgga aaatgattcg tggaaagaag cttttttaaa
     4321 atcaatcaca gtggaatatg ggagggataa gatcctgcaa aaaaatacac cgcattatcg
     4381 tttgatcggt ttgccgtttt ttactgatca tcagaaaaat ggacaattta cagagttatt
     4441 ccctttaggt gcggcatcgc ttgaaaaata gagtagtgca ttgcaggcag ccccgttcgg
     4501 caaaacttcc tttacaaaag ggcgttttgt cagatattta atcaacacat tattaaaata
     4561 caaccaaatt ttaatgccgt ccgaaccatg tattcagacg gcatcgtatt tttcagtatc
     4621 taaaccgttt ccctgcccca atccttactc ctcaaaatcg aggcatcgac atcttgaata
     4681 tcgcggtgtc ccgtaaacgc catcgacaca tccatttcct tgtacaaaat ctccagcgcg
     4741 cgcgttacgc cttcttcgcc atacgcaccc aaaccataca ggaacgcccg acctatcatc
     4801 gtgcctttcg cacccaaagc ccacgccttc aaaatatcct ggccgctgcg gataccgctg
     4861 tccatccaaa cttcgatgtc actgccgact gcggaaacaa tatcgggcaa ggccttgatg
     4921 gcggacacag tatcgtcgag ctggcggccg ccgtggttgg aaacgaccaa tgcatccgca
     4981 ccgcttttcg ccgccttttc cgcgtcttcg ggttccataa tgcctttgat aatcagcttg
     5041 ccgccccaca aatctttaat ccgcgccacg tcgtcccagc ttaagcgcgg gtcgaactgt
     5101 tccgccgtcc acgaagaaag tgaagacaaa tcgccgacgt ttttggcgtg tccgacgata
     5161 ttgcggaacg tgcggcgttc cgtgttcagc attttcatgc accattcggg cttggtcgcc
     5221 agattgatta aattggcgat ggtcggtttc ggcggcgcgg acaggccgtt tttgatgtct
     5281 ttgtggcgtt gacccaaaac ctgcaaatcg gcagtcaata ccaatgccga acatttggca
     5341 tctttcgcac gcttaatcag gttttccata aattcgcggt cgcgcatcac ataaagctga
     5401 aaccaaaacg gcgcgctggt gttctcggca acgtcttcaa tcgagcagat ggacatggtg
     5461 gacaacgtaa acggaatacc gaacttctcc gccgcacgcg ccgccaaaat ttcgccgtct
     5521 gcgtgtgcca tgccggtaaa acccgtcggc gcaatcgcca ccggcatttt cacatccccc
     5581 ccgatcattt tggtttcgag gctgcgtccc tccatattga ccaatacctt ttgtcggaag
     5641 cggatatctt tgaaatccga agtattctcc ctataagtcg tttccgtcca cgaacccgaa
     5701 tcgatgtaat cgtaaaacat acgcggcatt ttgcgcttgg caacgcggcg caagtcttcg
     5761 atgcaggtca ttttgctcaa atcacgtttc atcttgtttt tccctgttta acagcctgat
     5821 ggaatatgcc gtctgaaact gcttgagcct tcaaacggca tttcgggcaa tacccaaata
     5881 aatcactaag atttagataa tctatatcaa tattaaaaat aaatcaaatc gttttgaaac
     5941 cgcttgccgg caaaacgccc caaccgccgc tcctcaaaaa acataactaa ctgattcaaa
     6001 aaaccaaaca aataaagcaa aatcagacta taccgccatt taaacaaaca atcggcaaca
     6061 gctcccccat acttgactga aacactcaga tattggacaa ttccgcccac tataaaaaag
     6121 ccgacacggg caaccaccac catgagactg accaccaaag ggcgtttcgc cgttaccgct
     6181 atgctggatt tggcgatgaa cgcgcaaacc ggcgccgtca aactcagtgc catcagcgaa
     6241 cgccaaaata tatccctctc ctatctcgag caattgttcg gcaaactccg ccgcgccgga
     6301 ctggttgaaa gcctgcgcgg gcccggcggc ggctacatcc tcgccgcccc tcccgcacaa
     6361 atcaacatcg cccaaatcat ttccgccgcc gaagaccgtc tggacgcgac ccaatgcggc
     6421 agcaaagcca actgccacca cggcgcgccc tgcctgacgc acgacctttg ggagaattta
     6481 aacaaaacca tcaacgacta tctcggcagc gttaccctgc aaagcatcat cgaacagaaa
     6541 aacaacggcg acggcagccg cgtcgtccaa tttacacaca tccattaaat aacacccgaa
     6601 aaagaaagag caaaccatga ccgtcaaaac ccccgtttac ctcgactacg ccgccaccac
     6661 acccgttgac aaacgcgttg ccgaaaaaat gattccctat ctgactgaaa ccttcggcaa
     6721 ccccgcctcc aacagccacg cattcggctg gacggcagaa gaagccgtcg aaaaagcccg
     6781 cgccgacatc gccgccctga ttaacgccga ccccaaagaa atcgtcttca ccagcggcgc
     6841 gaccgagtcc gacaacctcg ccatcaaagg tgcggcaaac ttctacaaaa ccaaaggcaa
     6901 acacctcatc accgtcaaaa ccgaacacaa agccgtgctc gacacgatgc gcgaactcga
     6961 acgccaaggc tttgaagtta cctacctcgg cgtgcaggaa aacggtttga ttgatttgga
     7021 agaactcaaa gccgccatcc gcgacgacac catcctgatt tccataatgt gggtgaacaa
     7081 cgaaatcggc gtggtgcaaa acattcccgc catcggcgaa atctgccgcg aacgcaaaat
     7141 cgccttccac gtcgatgccg cccaagcctg cggcaaagtg cctgtcgatg tcgaagccgc
     7201 caaaatcgac ttgctctcga tgtccgcgca caaagtgtac ggccccaaag gcatcggcgc
     7261 gctgtacgtc cgccgcaaac cgcgcgtccg cctcgaagcc caaatgcacg gtggcggtca
     7321 cgagcgcggt ttccgttccg gcacattgcc gacccatcaa atcgtcggca tgggcgaggc
     7381 cttccgcatc gccaaagaag aattggcaca agacacagcg cactacctga aactgcgcga
     7441 catcttcctc aaaggcatcg aaggcatcga agaagtctat atcaacggcg acctcgaaca
     7501 ccgcgccccg aacaacctga acgtcagctt caacttcgtc gaaggcgaaa gcctgattat
     7561 ggcggtgaaa gaactcgccg tatccagcgg atccgcctgc acctccgcct cgctcgaacc
     7621 cagctacgtc ctgcgcgcgc ttggccgcaa cgacgaactg gcgcactcat ccctgcgcat
     7681 caccttcggc cgcatgacca ccgaagaaga agtgcaattc gcggcagaac tgatcaaatc
     7741 caaaatcggc aaactgcgcg aactgtcgcc gctgtgggaa atgttcaaag acggtattga
     7801 tctgaactcg attgaatggg cggcgcatta agattgatgt caaacaaagg ctgtctgaga
     7861 aagaatacca tgaaagttaa aattattaat ttgccaaatg gttataaacg tattatttat
     7921 ggaaaatatt ttgaacaatt tgacttagat tatgaacaag atttagacgt attaaaaaaa
     7981 gatattgaat ttgctttatc tgttattgaa tacaatcgat ctatattcaa aaaattctct
     8041 tccttatttg agaataaaat catttttgtt tatcaaggag gacatcatct tgatattatt
     8101 gatcgtgata aaggaagttt gaaatagtta actattgaag atcattggac aaaagatgtt
     8161 gatgatatca ctggttcatg gaaaatggag aatttagaaa ttagccgaca aactcaatat
     8221 gcaatgcaac ttctaaagat tggtaaaaca aatattgaga aagaaataag aaaaattaaa
     8281 caaaggttct aaaaaaatga gtaaatacgg tggtgaagac gtaatctata tgaatgatgg
     8341 tgtatttaga aataaattag ccattcaaga tccacttaaa tttaaaaaag gtcgaaagag
     8401 acatttctgc tatttccgcc caaaatctgc ttttgcaacc cattacaggc aatttcgatt
     8461 tggcgcattt acagaccatt catcgcgaac tgtttggcaa tgtttatgat tgggcaggaa
     8521 aaatccggag ggttgatatt tctaaaggta atacccgatt tgccaatttt gcttttatcg
     8581 aaaacgaaag ccgtaaactt ttggagaagc tgaaaaacga aaactatttg cgggtgttgg
     8641 ataaagataa attcgccgag cgtgcagcct attatttgga cgagttgaac gtcctacatc
     8701 cttttcgtga aggaaacggc agaacattgc gcctgtttat gacacaattg gcaataaaaa
     8761 acggttttca gatacattgg caaaatatct ctgccgaaca aatgattcaa gcctgtattc
     8821 aggcatacca tgccgatagc agcttattgg cacgcctaat tattagacaa tttagaacaa
     8881 tcgtcttttt cagacgacta aaacatcaac aaaccacgat attttaagga aaccacatca
     8941 tggcatacag cgataaagta atcgaccact acgaaaatcc ccgcaacgtc ggcactttcg
     9001 acaaaaacga cgagtccgtc ggcaccggca tggtcggcgc gcccgcctgc ggcgacgtga
     9061 tgcgcctgca aatcaaagtg aacgatgaag gcatcatcga agatgcgaaa ttcaaaactt
     9121 acggctgcgg ttccgccatc gcttcgtcca gcctgattac cgagtgggtc aaaggcaaaa
     9181 gtctggatga cgcgctggca atcaaaaaca gcgaaatcgc cgaagaactg gaattgccgc
     9241 cggtaaaaat ccactgctcc atcttggctg aagatgcggt aaaagcggcc gttgccgact
     9301 accgcaaacg tcaggaaaac agataaagcc cttcagacgg catcgtccca caatgccgtc
     9361 cgaaccaccc gccgcttcgg atccgtccgg ggcggtacaa caaggaagaa atatgattac
     9421 ccttaccgag aatgccgcaa aacacatcaa tgactatctc gccaaacgcg gcaaaggctt
     9481 gggcgtacgc ttgggtgtaa aaaccagcgg ctgctcgggg atggcgtaca accttgaatt
     9541 tgtcgatgaa gccaacggcg acgacctgat tttcgaagga cacggcgcgc gcatttatat
     9601 cgacccgaaa agcttggttt atctggacgg cacacaagtc gattacacca aagaagattt
     9661 gcaggaaggt ttcaaatttg aaaaccccaa tgtcaaagac tcctgcggct gcggcgagag
     9721 cttccacgtt taaggcataa aaacggcggg acagtatcaa aaccgtcccg ccgttttttc
     9781 gcttcctgcc tgttatagct gcctttgcct ttccttttcc gttccacctt gtgccggaac
     9841 aaatcggatt ttactaaggc ttttaaagca ttgtcgcgta ttttgccttt attgtgctgc
     9901 gctttgccgc ccatattcag tcctttcgtt taagaagccg cagattataa ggcaaaaaca
     9961 gttttctgcc aaaatcttac atttatcatc ctactatgtc ccaatatttc accctcttcc
    10021 ggattgaacc cgctttcgat atcggcaccg aaaacttgga acaaacctac cgcgccttgg
    10081 ccgcccgttt ccatcccgac aaattcgctt ccgcttccgc tttcgagcag aaacaggcgg
    10141 ttatgatgtc ttccaccatc aacgatgcct accgcacctt gaaaaatccc atcgaccgcg
    10201 ccgcgtacct gctgaaaaca tcgggtatcg atgccgatgc gccggaacat acctctttcg
    10261 cccccgactt cctcatgcag caaatggaat ggcgcgaaac gctgatggag gcacgggcag
    10321 gcaacaacct tgaatccttg aaaaatctcg ataacgaaat ccgcgccgaa caagaaaaac
    10381 tgttctgcgg tctgaaacag tcattcgcgc gccaagactg cgacaccgcc gcacaacaag
    10441 tccgccaagg caggtttctc gacaaactcc gccacgaaat ttcctccgca ttataatccg
    10501 caccgtgttt cagacggcat aaccgccgca ccgtttcatg tcgaaatatg ctaaaataag
    10561 caacaatttt ttgccatacg aaacattgaa accatgaccg acgcaaccat ccgccacgac
    10621 cacaaattcg ccctcgaaac cctgcccgtc agccttgaag acgaaatgcg caaaagctat
    10681 ctcgactacg ccatgagcgt cattgtcggg cgcgcgctgc cggacgttcg cgacggccta
    10741 aagccggtgc accggcgcgt actgtacgcg atgcacgagc tgaaaaataa ctggaatgcc
    10801 gcctacaaaa aatcggcgcg catcgtcggc gacgtcatcg gtaaatacca cccccacggc
    10861 gattccgcag tttacgacac catcgtccgt atggcgcaaa atttcgctat gcgttatgtg
    10921 ctgatagacg gacagggcaa cttcggatcg gtggacgggc ttgccgccgc agccatgcgc
    10981 tataccgaaa tccgcatggc gaaaatctca catgaaatgc tggcagacat tgaggaagaa
    11041 accgttaatt tcggcccgaa ctacgacggt agcgaacacg agccgcttgt actgccgacc
    11101 cgtttcccca cactgctcgt caacggctcg tccggtatcg ccgtcggtat ggcgaccaac
    11161 atcccgccgc acaacctcac cgacaccatc aacgcctgtc tgcgtctttt ggacgaaccc
    11221 aaaaccgaaa tcgacgaact gatcgacatt atccaagccc ccgacttccc gaccggggca
    11281 accatctacg gcttgggcgg cgtgcgcgaa ggctataaaa caggccgcgg ccgcgtcgtt
    11341 atgcgcggta agacccatat cgaacccata ggcaaaaacg gcgaacgcga agccatcgtt
    11401 atcgacgaaa tcccctatca ggtcaacaaa gccaagttgg tcgagaaaat cggcgatttg
    11461 gttcgggaaa aaacgctgga aggcatttcc gagctccgcg acgaatccga caaatccggg
    11521 atgcgcgtcg ttatcgagct gaaacgcaac gaaaatgccg aagtcgtctt aaaccaactc
    11581 tacaaactga ctccgctgca agacagtttc ggcatcaata tggttgtttt ggtcgacgga
    11641 caaccgcgcc tgttaaacct gaaacagatt ctctccgaat tcctgcgcca ccgccgcgaa
    11701 gtcgttaccc gacgtacgct tttccggctg aagaaggcac gccatgaagg gcatatcgcc
    11761 gaaggcaaag ccgtcgcact gtccaatatc gatgaaatca tcaagctcat caaagaatcg
    11821 cccaacgcgg ccgaggccaa agaaaaactg cttgcgcgcc cttggcgcag cagcctcgtt
    11881 gaagaaatgc tgacgcgttc cggtctggat ttggaaatga tgcgtccgga aggattggct
    11941 gcaaacattg gtctgaaaaa acaaggttat tacctgagcg agattcaggc agatgctatt
    12001 ttacgcatga gcctgcgaaa cctgaccggc ctcgatcaga aagaaattat cgaaagctac
    12061 aaaaacctga tgggtaaaat catcgacttt gtggatatcc tctccaaacc cgaacgcatt
    12121 acccaaatca tccgtgacga actggaagaa atcaaaacca actatggcga cgaacgccgc
    12181 agcgaaatca acccgttcgg cggcgacatt gccgatgaag acctgattcc gcaacgcgaa
    12241 atggtcgtga ccctgaccca cggcggctat ataaaaaccc agccgaccac cgactatcag
    12301 gctcagcgtc gcggcgggcg cggcaaacag gcggctgcca ccaaagacga agactttatc
    12361 gaaaccctgt ttgttgccaa cacgcatgac tatttgatgt gttttaccaa cctcggcaag
    12421 tgccactgga ttaaggttta caaactgccc gaaggcggac gcaacagccg cggccgtccg
    12481 attaacaacg tcatccagct ggaagaaggc gaaaaagtca gcgcgattct ggcagtacgc
    12541 gagtttcccg aagaccaata cgtcttcttc gccaccgcgc agggaatggt gaaaaaagtc
    12601 caactttccg cctttaaaaa cgtccgcgcc caaggcatta aagccatcgc actcaaagaa
    12661 ggcgactacc tcgtcggcgc tgcgcaaaca ggcggtgcgg acgacattat gttgttctcc
    12721 aacttgggca aagccatccg cttcaacgaa tactgggaaa aatccggcaa cgacgaagcg
    12781 gaagatgccg acatcgaaac cgagatttca gacgacctcg aagacgaaac cgccgacaac
    12841 gaaaacaccc tgccaagcgg caaaaacggc gtgcgtccgt ccggtcgcgg cagcggcggt
    12901 ttgcgcggta tgcgcctgcc tgccgacggc aaaatcgtca gcctgattac cttcgcccct
    12961 gaaaccgaag aaagcggttt gcaagtttta accgccaccg ccaacggata cggaaaacgc
    13021 accccgattg ccgattacag ccgcaaaaac aaaggcgggc aaggcagtat tgccattaac
    13081 accggcgagc gcaacggcga tttggtcgcc gcaaccttgg tcggcgaaac cgacgatttg
    13141 atgctgatta ccagcggcgg cgtgcttatc cgtaccaaag tcgaacaaat ccgcgaaacc
    13201 ggccgcgccg cagcaggcgt gaaactgatt aacttggacg aaggcgaaac cttggtatcg
    13261 ctggaacgtg ttgccgaaga cgaatccgaa ctctccggcg cttctgtaat ttccaatgta
    13321 accgaaccgg aagccgagaa ctgaaaatca tctcccgatg ccgtctgaag attcagacgg
    13381 catttatttt tccctcatcc gtcatccagc ttctcacaat atagcggatt aacaaaaatc
    13441 aggataaggc gacgaagccg cagacagtac aaatatagtg gattaaattt aaactagtac
    13501 agcgttgcct cgccttgccg tactatagtc aattaaaaac aagggactgt actagattat
    13561 ccctaaattc cacaccgatc ccgcaggatt tttagctgcc gggacggtgt gccgaagtta
    13621 aatcgaaatt c
//
LOCUS       DBJGGI010000055        13563 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.055, whole genome shotgun sequence.
ACCESSION   DBJGGI010000055 DBJGGI010000000
VERSION     DBJGGI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13563
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.055"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(174..383)
                     /locus_tag="ACSIGJ_001588"
     CDS             complement(174..383)
                     /locus_tag="ACSIGJ_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HHQ1330421.1"
                     /translation="MTDFADRASEREAEFLVEALAKHQPPSENTAGFSHCEDCGDPIP
                     EAKRKAVRGCTRCVVCHEYSQLKTK"
     gene            complement(376..597)
                     /locus_tag="ACSIGJ_001589"
     CDS             complement(376..597)
                     /locus_tag="ACSIGJ_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330422.1"
                     /translation="MPSDTPIKTVAVAEIPPVPSGLLVEYERPERPAGGSPEQLLNHA
                     VRYGGYYRKLEIQIEGWQNWHTKGRLKHD"
     gene            complement(671..958)
                     /locus_tag="ACSIGJ_001590"
     CDS             complement(671..958)
                     /locus_tag="ACSIGJ_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330423.1"
                     /translation="MIEFVRAKKRLLWAFVLLLAWACGYRYAADKAEAEQTALIAAYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQSTDLAAALSELDKTRNTLQE"
     gene            complement(1078..1716)
                     /locus_tag="ACSIGJ_001591"
     CDS             complement(1078..1716)
                     /locus_tag="ACSIGJ_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330424.1"
                     /translation="MTIQSKHYEIRPKQAFITPENVSIPADLCCEVQVRSLLQHAYAE
                     LVHDNIYKPDGNVPKQAEREVAKSMALMETTDDLFSRTLAILKEANQPQEELLPQLSQ
                     LYQKEIGLVPEVDKKTNMIFLETFQSSISQSSILSDIRSLLNEKKYIAKRIKENAEEM
                     YFFSQPAALLVYWLIEKVGADEVWKKWPLPAYNKNLKFICTDLDKQPSHELF"
     gene            complement(1713..2087)
                     /locus_tag="ACSIGJ_001592"
     CDS             complement(1713..2087)
                     /locus_tag="ACSIGJ_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330425.1"
                     /translation="MSDIDDFKSYLESHQAAFSAWGRFVAEEIQNQLSNVISPVPVAN
                     FLKIEAKPGVKEISSALAKIGRKNYTSPQTQMTDLVGVRFVALLAEHIQIVCEIIESS
                     SQWNAKVSKDFADEIQQNPKAI"
     gene            complement(2084..3124)
                     /locus_tag="ACSIGJ_001593"
     CDS             complement(2084..3124)
                     /locus_tag="ACSIGJ_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HHQ1330426.1"
                     /translation="MKKIITHRIYPKNENRERVEPKISTHLIDLPITARRTLETRLTK
                     ALGNKSHGIEMSVVNTAENSFFQIAAAIQLKEEAEFIEDSAQFAHMLTDAQLNTNAPG
                     GILLVLKGRVGDTGKPFLCVIKAEPQDGFRTKEEDDFITIEFLEELLLTDSARLFKIG
                     FLVAETVRPLEQIQSGNYRAFLYDHLMTQTETRPAASYFYQVFLGMSIAASSRKLTQD
                     FFEWTRNFIDNSDLSDDAKLDAHEALRVTLKSAEATISVNNFAQNHLPQEKRTTYTEF
                     MVEKDFPQNAVSKDIEYIKTRLRKRRSYGFSNGVVILTPPEHTQDYMEIAPTEDGEYT
                     VVLIKGQLQQQK"
     gene            complement(3358..3738)
                     /locus_tag="ACSIGJ_001594"
     CDS             complement(3358..3738)
                     /locus_tag="ACSIGJ_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHQ1330427.1"
                     /translation="MEKTSLFGKRLKEERIKLGLNQAEAAEKCGFSREMWGKWERGEN
                     RPSSEKLFSFSKIGIDIDYVMHGRRGETAAMPSESLSAEEKELLALFRQLGSGSRKEL
                     ADYAAFKLVVEKKAQTALGKVSNG"
     gene            4010..4240
                     /locus_tag="ACSIGJ_001595"
     CDS             4010..4240
                     /locus_tag="ACSIGJ_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HHQ1330428.1"
                     /translation="MEKPLNFKPIPYPQTRGSTAVWFKRNGVCKTHRAKHFNLERTAV
                     EHPLRGKLKGNFGKSHEAAVKFGLKEDADGDE"
     gene            4227..4511
                     /locus_tag="ACSIGJ_001596"
     CDS             4227..4511
                     /locus_tag="ACSIGJ_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HHQ1330429.1"
                     /translation="MATSKKAQRLLRVFLAMDAHPVIGISNKELSDGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRKQQERLNARLQELENRIY"
     gene            4548..5421
                     /locus_tag="ACSIGJ_001597"
                     /pseudo
     CDS             4548..5421
                     /locus_tag="ACSIGJ_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5414..7180
                     /locus_tag="ACSIGJ_001598"
     CDS             5414..7180
                     /locus_tag="ACSIGJ_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HHQ1330430.1"
                     /translation="MIERLKAVENQAEAMGRGARSAYLKQQAQELGISLATLYRKLEA
                     VSVKPTRKRRSDAGKTELKPEEAKLISAVLAEAMRRNGKRLMPVRQAVEMLRANGKIE
                     AARIDGETGEVIPLSENTITRALREYKLHSDQLLQPDPVSRMKSEHPNHCWQIDPSLC
                     VLYFLPRQGKDTGLRVMKEEEFYKNKPKNVVKIENDRVWRYTGTDHASGTISVRYYFG
                     GETSANLCDFFIYMMQAKKDIGKDPFRTVPRMVMLDPGSANTSAAFKNLCKPLDVHVQ
                     INKPGNPRAKGQVEKANDIVETAFESGLRFTEVHDIDQLNALSERWMRYYNGTQKHSR
                     HGMTRYQAWNKIKPEQLILPPPAEYCRELAVSAPKEAKVSADLEIRFGGRVYSVKGIK
                     GILVGKKVLVGKNPWEANGARVATYDAEGNEVWVSVPEVVFDEMGFKADAAVIGAEYK
                     APADTDAQRHRKELDKLAMGAETLEAAAAKRKGKAVPFGGKIDPYKHQEDTLAASNTL
                     FMPKQGQQMDYNKMEVAEQVLSKVEIAKRLKPRVEADGGDWKQAVSVILKHYPEGVTE
                     GRLEEAFERTRTRCRLKLLKTG"
     gene            complement(7221..7409)
                     /locus_tag="ACSIGJ_001599"
     CDS             complement(7221..7409)
                     /locus_tag="ACSIGJ_001599"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330431.1"
                     /translation="MPSEFFQTAFLPTRFACRVGLPVRPEASDKQGGFYSGLNLNRYG
                     VASPCRTICTVCGFAALS"
     gene            complement(7519..8906)
                     /locus_tag="ACSIGJ_001600"
                     /pseudo
     CDS             complement(7519..8906)
                     /locus_tag="ACSIGJ_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238907.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            9024..9100
                     /locus_tag="ACSIGJ_001601"
     tRNA            9024..9100
                     /locus_tag="ACSIGJ_001601"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9058..9060,aa:Arg,seq:acg)
     gene            9118..9192
                     /locus_tag="ACSIGJ_001602"
     tRNA            9118..9192
                     /locus_tag="ACSIGJ_001602"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9151..9153,aa:Glu,seq:ttc)
     gene            9355..9471
                     /locus_tag="ACSIGJ_001603"
                     /pseudo
     CDS             9355..9471
                     /locus_tag="ACSIGJ_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            9629..10804
                     /locus_tag="ACSIGJ_001604"
     CDS             9629..10804
                     /locus_tag="ACSIGJ_001604"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HHQ1330432.1"
                     /translation="MQNIFDPLIIRGKSLIPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKNLGLEGEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLASAETEKVV
                     EFMSVAGLPARGIRTKFLDSYIRREGKLQANAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDQLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(11146..11694)
                     /locus_tag="ACSIGJ_001605"
     CDS             complement(11146..11694)
                     /locus_tag="ACSIGJ_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HHQ1330433.1"
                     /translation="MSQNNPIKPWYKHVWPWVLMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLPPANHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(11697..13193)
                     /gene="ccoG"
                     /locus_tag="ACSIGJ_001606"
     CDS             complement(11697..13193)
                     /gene="ccoG"
                     /locus_tag="ACSIGJ_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HHQ1330434.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKVKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPSGLI
                     RYTTESALEHEYAEKDIKKRLLRPRVAGYGAVLAVVVAAFLVGLSTRKMVEVDILKDR
                     GVMVRENAKGWLENAYSLRIINNSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETI
                     TLPVQVSTIPEYADKGSHPIEFIFQYRESGASDGKPVVLEEDATFIGE"
BASE COUNT         3581 a         3390 c         3271 g         3321 t
ORIGIN      
        1 aagaggacga ttccctaagg taatgaagcg ccgggcggat cggttccgta ccattcgtac
       61 tgcctgcggc ccgccgcctt gtcctgattt ttgttaatcc gctatggttt aaggctttgt
      121 ttcttaagtc cgcagagtat gccatggtta gaccttcaac gtcgagtgtt gtactatttt
      181 gtttttaatt gactatattc gtggcagaca acacagcgcg tacagcctcg gacggctttt
      241 cgctttgctt ccggtatcgg gtcgccgcag tcttcgcaat ggctgaagcc ggcggtgttt
      301 tcagacggcg gttggtgttt cgccaaggct tccaccagaa attcggcttc gcgttcggat
      361 gcgcggtcgg caaaatcagt catgtttcag acggcctttc gtgtgccagt tctgccagcc
      421 ctcaatttgg atttcgagct ttcggtagta tccgccatag cggacggcgt ggtttaaaag
      481 ttgttcggga gagccgccgg ccggacgctc ggggcgttcg tattcgacca gcagtccgga
      541 aggaacgggc ggaatttccg cgacggcaac ggttttaatc ggggtatccg aaggcacggt
      601 tgtagaggtg caggctgttc gtgccaaggc cgttaaaacg gttgccgtct ttgtctgccg
      661 cttcacgggt ttactcctgt aaggtgttgc gcgttttgtc caattcgctc agggcggccg
      721 ccaaatccgt gctttgtttt tgggaaaagt cgtaccaccg ctgcctttcg tcctgcgctt
      781 ttttaagctg caaggcgtat tgttccgccg caaccataga agaatgccga taggcggcaa
      841 tcagggcggt ttgttccgct tcggccttgt cggcagcgta ccggtagccg cacgcccacg
      901 caagcaaaag tacaaatgcc caaagcagcc gttttttggc tcggacaaat tcaatcattg
      961 ccgccccctt cctgatttgc ggattttttg atttccgcca gctgcggaac tgccgctatg
     1021 ccgcgtttga ttaaggcata attaggaaag gccgtctgaa tgggctttta aagggggtta
     1081 aaagagttca tgggatggtt gcttatccaa atcagtacaa atgaatttca ggtttttgtt
     1141 gtatgcaggc aaaggccatt ttttccatac ttcatctgca ccaactttct caatcagcca
     1201 atagaccaac aaagctgctg gctgactgaa aaaatacatt tcttcagcat tttcttttat
     1261 ccgcttggca atatatttct tctcattcaa aagtgaacgg atgtcagaaa gaatacttga
     1321 ttgggaaatg ctggattgaa atgtttctag aaaaatcata ttggtttttt tatcaacttc
     1381 aggaacaagc cctatttcct tttgataaag ctgtgataat tggggaagaa gctcttcttg
     1441 tggttgattt gcttctttca aaattgccag agtacgacta aataagtcat ctgtagtttc
     1501 catcaaagcc atgctcttcg cgacttcgcg ctctgcttgc tttggaacat tgccgtcagg
     1561 cttgtatata ttgtcatgaa ccagctctgc ataagcatgt tggagtaaag aacgcacctg
     1621 aacttcacag cacaaatcag cagggatgct gacattttcg ggtgtaatga atgcttgttt
     1681 aggacgaatc tcgtaatgtt tggattgtat agtcaaattg ctttgggatt ttgctgaatt
     1741 tcatctgcaa aatctttcga aactttagca ttccactgag atgaggattc aatgatttca
     1801 caaactattt gaatatgttc cgcaagcaat gccacaaaac gtacgccgac caagtcagtc
     1861 atttgagttt gaggtgaagt gtaatttttt cggccaatct tggctagagc tgaagaaatc
     1921 tctttgactc caggctttgc ttcaattttt agaaaattgg caaccggaac aggagaaatg
     1981 acattggata attgattttg aatttcttca gccacaaatc taccccaagc ggaaaatgcc
     2041 gcttggtggc tttctaaata gcttttgaaa tcatcaatat cactcatttt tgttgttgta
     2101 actgtccttt aattaggaca acagtatatt ccccatcttc cgttggcgca atttccatat
     2161 agtcctgagt atgctcggga ggagtcaaga taactacacc gttactaaat ccgtaagacc
     2221 tccgtttgcg taaacgagtt ttaatatatt caatatcttt acttacggca ttttgaggaa
     2281 aatccttttc caccataaat tctgtataag ttgttcgttt ttcttgaggt aaatgatttt
     2341 gggcaaaatt atttacacta atggttgctt ccgcactttt caatgtaacg cgcaatgctt
     2401 catgcgcatc taattttgca tcatcactta aatcagagtt atcgataaaa ttgcgtgtcc
     2461 actcaaaaaa atcctgcgtc aatttacggg aagaagcagc tatactcata cccaagaata
     2521 cttgatagaa ataggaagct gccggtctag tttccgtttg tgtcatcaga tggtcataca
     2581 aaaaagctcg ataattccca gattgtattt gctctagcgg ccttactgtt tcagccacca
     2641 aaaaacctat cttgaataat cttgctgaat cggtcagtaa taattcttct aagaattcaa
     2701 tcgtgataaa gtcatcctct tctttggttc ggaacccatc ttgaggttca gccttaatta
     2761 cacataaaaa cggcttaccg gtatctccaa ccctaccttt taaaaccaac aaaataccac
     2821 ccggtgcatt tgtattcagt tgtgcatcgg tcaacatgtg ggcaaattgt gcggaatcct
     2881 caataaactc tgcttcctct ttgagttgta tggctgccgc tatctgaaaa aaactatttt
     2941 cagccgtatt gacaacagac atttcaatgc catgagattt attacccaaa gctttggtca
     3001 gcctggtttc caatgttctt cttgccgtaa taggcaaatc aatcaaatgt gtactgattt
     3061 taggttctac acgctctcta ttttcatttt tcggataaat acgatgggta ataatttttt
     3121 tcaattacca gcccttcaaa agagtaattc gtttctgtca tcatttttcc tattgtaagt
     3181 taattaaggt cttttgaaat ctgaaccact tggccgatga cctgaatatc ggggtggttt
     3241 ttaatacgct ttaaaatgca cggccgctcc tattgcttaa tatctgtttc agaacaccgg
     3301 agcagcccga ctgtcagagt gtcccaaatg ccgtctgaaa tcttcagacg gcatttttta
     3361 tccgttgctc actttaccaa gcgcagtttg ggcttttttc tctaccacca gcttaaaggc
     3421 ggcgtagtca gcaagctctt tgcggctgcc gctgccgagc tgacggaaca gggcaagcag
     3481 ttctttttct tcagcactca aggattcaga cggcatggcg gctgtttcgc cgcgtctgcc
     3541 gtgcatgacg taatcaatat cgatgccgat tttagaaaaa gagaataatt tttcacttga
     3601 tgggcgattt tcaccacgct cccactttcc ccacatctca cgagaaaaac cacatttttc
     3661 agcagcttct gcttggttca agcctaattt aattctttct tctttcaatc gcttaccaaa
     3721 aagagaagtt ttttccactt aaatttcctt gaaaagagaa atatttttct cataattcac
     3781 acatcaagac atgaaacacc ctaaatcatc acgaaaaagg acgaaaaaat gaacactttg
     3841 aaggctcatg cgcgacagct catgatggat cacatacacc cggctacggg aaaaagcttg
     3901 tttattttaa aacaagccaa gaccctgctg ctgcacaaca gaccggatgt ttatcgtgca
     3961 aagaggccta aaactaaaca cattatacca cacgaaaggc ataaataata tggaaaaacc
     4021 tttaaatttc aagcctattc cctatccgca aacgcgcggc agcacggccg tgtggtttaa
     4081 gcgcaacggc gtgtgcaaga cgcaccgggc gaaacatttc aatttggagc gcaccgcggt
     4141 ggaacacccc ttgcgcggca agctgaaagg caatttcggc aaaagccacg aagcggcggt
     4201 aaaatttggg ctgaaggagg atgcggatgg cgacgagtaa aaaggcgcag cggctgttgc
     4261 gggtgttttt ggcgatggac gcgcatccgg taatcggcat cagcaataag gagctttcag
     4321 acggcctggg gctgacgcca tcacaagtca gcagggatat tgatgatttg gttgcatcgg
     4381 gattggtcat caagcttgaa aacggcaact acgcctacgg catcaaaacc ctacaaatcg
     4441 cagagcggtt cagaaaacag caagagcggc tgaatgcgcg cttgcaagaa ttggaaaacc
     4501 gaatttacta aatgcgacga cgacgtcgca tttgaggagc aaaagaaatg gcaacagaaa
     4561 ttttaggaca tacggtcggc gtaacggcaa acgaattggc tatccacagt atggaggtta
     4621 tggacaagtt ttcaggcggc gaggcctaca acgagacggt atggattgag cgcggacgat
     4681 ttgcggacgc caaacgatgg aagggatgtt tgagccggga cgcgcgctga tcatcatcaa
     4741 agagcatacg ccgcatgggc ggtttgccga aatcgctgaa aaagagttgg cctcggacgg
     4801 cgggaatccc aaagactgat gaatgccacc ctccgtttta tcgacccgaa aatgaaacaa
     4861 gcgcagccta agctgatgac gttgggcaaa tccaaactgc tcgagctgct ggtcgaagac
     4921 gacgacactt tgttggagct tgccgaaggc ggtgaggtca acggcaacac ttttgacgat
     4981 gtcgaccgta tgactattaa ggagctgcgc gtcgccctgc gcgaaagccg tgaaacggcg
     5041 gaagcgaaag ataaggtgat tgccgataaa aataaaaagg tcgatgagct ggcggaaaag
     5101 ctgtcgaaaa agcagacggg tgtcaaagag cctaaacctg cggatgtggg catcgagctg
     5161 acgatgcagc ttggcagctt ggaagtcggt atccgctcgc aaatcagccg attgcgcgat
     5221 atgttcgagc agatggcggc gcacggcgag gcgcatggat ttgaccaccg cgcgaagatg
     5281 gtcggcacgc tcaatcaaat tattttggac tgcgagcaac tgcgcgaaag ctatgcccta
     5341 ccgaccgaag caccggcaga caatgtgccg gaatggttgg gcggtgaaac gggagaaggc
     5401 gatgaatccg gcaatgattg agcgtcttaa agcagtcgaa aatcaggcgg aagcaatggg
     5461 gcgcggcgca cgctctgcat atcttaagca gcaggcgcag gaattgggca tcagccttgc
     5521 cacgctatac cgcaagctgg aggcggtcag cgtcaagccg acgcgcaaac ggcgcagcga
     5581 tgcgggcaag acggagctta aaccggaaga agccaaattg atttcggcgg ttttggcgga
     5641 ggcgatgagg cgcaacggca agcggttgat gccggtgcgg caggcggtgg aaatgctgcg
     5701 cgccaacggg aaaatcgagg cggcgcggat tgacggggaa accggggaag tcatccccct
     5761 ttctgaaaac accattaccc gggctttacg ggaatacaag ctgcattccg accagctttt
     5821 gcagcccgac cccgtcagcc gcatgaaatc ggaacacccg aaccattgtt ggcaaatcga
     5881 cccgagtttg tgcgttttgt atttcctgcc gcgtcagggc aaggatacgg ggctgcgggt
     5941 gatgaaggaa gaggagtttt ataaaaacaa gccgaaaaac gtcgtcaaaa tcgaaaacga
     6001 ccgcgtttgg cggtacacgg ggacagacca tgcctccggc acgatttcgg tgcgttatta
     6061 cttcggcggc gaaaccagcg cgaacctctg tgattttttc atctacatga tgcaggcgaa
     6121 aaaagacatc ggcaaagacc cgttccggac cgtaccgcgc atggtcatgc ttgacccggg
     6181 cagcgcgaat acttcggcgg cgtttaaaaa tttgtgcaag cccttggatg tgcatgtgca
     6241 aatcaacaag ccgggcaatc cgcgtgccaa agggcaagtg gaaaaagcca acgatattgt
     6301 ggaaacggca tttgagagcg ggttgcgctt taccgaggta cacgacatcg accagctcaa
     6361 tgctttatcg gaacgctgga tgcgttacta caacggtacg caaaaacaca gccgccacgg
     6421 catgacccgc tatcaggcgt ggaacaaaat caaacccgag cagctcatcc tgccgccgcc
     6481 tgccgaatat tgccgagagc ttgccgtcag cgcgccgaaa gaggcgaaag tctcggcgga
     6541 tttggaaatc cgctttggcg gacgggtata tagcgtaaaa ggcatcaagg ggattttggt
     6601 cggtaagaaa gttttggtcg gtaagaatcc ttgggaggca aacggggcgc gggtcgccac
     6661 ttatgacgcg gagggtaacg aggtttgggt atccgtaccc gaagtagttt ttgacgagat
     6721 gggcttcaag gctgatgccg cggtcatcgg ggcggaatac aaagccccgg ccgatacaga
     6781 cgcgcaacgg catcgcaaag agctggacaa gctggcgatg ggtgcggaaa cgctggaggc
     6841 ggcagccgcc aaacgcaaag gcaaggcagt cccgttcggc ggcaaaatcg acccgtacaa
     6901 acatcaagaa gatacgcttg ccgcaagcaa tacgctcttt atgcccaaac aggggcaaca
     6961 gatggattac aacaagatgg aggtcgccga gcaggtattg agcaaggtcg aaatcgccaa
     7021 acgcttaaaa ccccgcgtcg aggcagacgg cggcgactgg aaacaggcag tgtcggtcat
     7081 cctcaaacac tacccggaag gtgtgaccga gggcaggctg gaagaggcgt ttgaacgcac
     7141 ccggacaaga tgccgtctga aacttttgaa aaccggttag gtttcaaccg aaaacagctt
     7201 ttatagtgga ttaacaaaaa tcaggacaag gcggcgaagc cgcagacagt acaaatagta
     7261 cggcaaggcg aggcaacgcc gtaccggttt aaatttaatc cactataaaa accaccctgc
     7321 ttgtccgatg cctcgggacg gacgggcaaa ccgacacggc acgcaaaccg cgtcggcaaa
     7381 aatgccgtct gaaaaaattc agacggcatc ttcagacaca ttacctgcaa acggcaacac
     7441 attccaatcc aaaccgatca ggcaatcaat acgaacggct gtttacatac ttaccagctt
     7501 tcaccaaccg gtatcgattt aaccgatttc cttaatattt ttcctgtccg ttttaaactt
     7561 cgccttaaac acatccggta aatctttatc gaaataccaa agcccgtcat ccatttccaa
     7621 tgcgccgccc attccatgca gaacgacttt tcccccgtta aaggatcggt ttcggtaatt
     7681 tccacctcat gcatttttcc ccaatccaaa acaggcaact tgtgtgcaaa cgccaaaact
     7741 tgttcgtcag aacggccgca cgccttatcg catcggcctg aaacatatac gggacaaggc
     7801 tcatcctctg cataaccgtc cggaaggata ccggctgagg caggacacaa tccgtatgtt
     7861 tccgcccacg acgacaaagc ccgtctcgcc gcctttttat tggcaaataa tccggtaggc
     7921 ggattatccg tcacaccgtt tttcaaagcc gctgttttcg cattcaacat gccgtctgaa
     7981 cctttttcaa acctgacggt cgtaaatgtt ttaagcagat ttttggcaga tacataacaa
     8041 cccgaatgat aacgtccgac caattccgct ttaatcttat atgcatgcag gctgcccaat
     8101 gcgggaaaaa aaaacggact tcctccgtat tgcaccaatc aaacgggggc ttttccggaa
     8161 tccaacaaag ccgcaacctc gccatatgcc cgttcaaacg taccgatata atttactttt
     8221 cccccgccgt cgaaacaggc aagcacacct gtaccgtcgg gcaaaccgta cagccgttcc
     8281 ctcaaccgtt cgggaagcgc ggcaggaaaa agtttcggat tcgtcaaacg gaaacactgc
     8341 ctgctccact cctcaagccc gtgtgccgac aggctgtatt ccaaataatc acacaacgcc
     8401 gatacatccg ccatcgcacg atgcctgtct tccacaacaa tccccaacct ttcgatgata
     8461 ccgtccaggc tgtgcttgta aaactgcgga tacagccgac gcgaaagctg cacactgcac
     8521 aaagcaggcg atgaaaatcc gatacccgca cgatgaaact catgctttaa aaacgtatag
     8581 tcgaaacggc tgttgtgcgc caccagcacg caccccttca ataccgaaaa caacccgccg
     8641 gcaatttcag caaacaccgg cgcgtcggca accatgccgt ctgaaatccc cgtcagttcc
     8701 gccacaaact tcggaatcgg tttttgagga ttgaccagcc attcatacct ctccgccctt
     8761 ccctgcccaa acttgaccaa agccacttcg gttaccctgt cttcatacag attgccgccc
     8821 gtcgattcca aatcaaccac ggcaacaggc attccaaacc gtaaaaacgc cttttccagc
     8881 aagggccagc gggaaacaac aatcatttta ttctctttaa attcaaacaa caaaccgata
     8941 ttttacactt ttaaggcatt tcatccaaca aaataattga cagaatccga tgattaccat
     9001 aaaattcaaa aatttattgc agcgcacccg tagctcagtt ggatagagta tctggctacg
     9061 aaccagaggg tcgggcgttc gaatcgctcc gggtgcgcca gtaagaaaat acaatacgcg
     9121 cccatcgtct agcggttagg acattgccct ttcacggcgg taaccggggt tcgattcccc
     9181 gtgggcgtgc cagattccaa atccccgaaa taatccctcg gggattttta ttgcctcggc
     9241 aattcgttac catatcttta cctacccact tcatcagaat ctcagacgta atcgaatcat
     9301 attcaaactt ttgccatgca aaccgatatc ccataaccgg atgcggtgtc cgttcaatat
     9361 agtggattaa atttaaatca ggacaatgcg acgaagcccc agacagtaca aatagtacgg
     9421 caaggcgagg caacgccgta ccggttgaaa tttaatccac tatattttgt ccgattggta
     9481 cagatataac agcatattcc aaagctcctt ctgcttttct gtttaccact gcaatattta
     9541 gagtataata ccaaatttga gtaatggttc taaaacaatt aaaaccattt ttcatgagcc
     9601 tgactgattc gtatattcgg agaaactgat gcagaatatt tttgaccctt tgattattcg
     9661 tggaaaatcc cttattccca tcgtgcaagg cggtatgggg gtcggtgttt ccgcatcggg
     9721 tttatccagc gcggtggcgc gtgaaaacgg catcggaacg attgccagtg tagatttgcg
     9781 ccaccttcac gaagacctgc tcgccgaatc ccaaatcaat ccgagtgaag agaaatatac
     9841 gtctttgaac tgtaccgcat tagacaggga aatccaaaaa gccaaaagtg cttcagaggg
     9901 aaaaggactg attgcggtca acgtgatgaa ggcggtcaaa gaccacgccg cgtatgtccg
     9961 ccaggcttgc gaatcagggg cggatgcggt cgtgatgggt gccggcctgc ctttagacct
    10021 gccggaaatg accgagggct atcataagga tgtcgcgctg ctgccgattc tgtccgaatc
    10081 gcgcggtatt aatatcgtct tgaaacgttg gatgaaaaaa ggcatattgc ccgatgcgat
    10141 tgtagtcgaa catcctgccc atgcggccgg acatttgggt gcatcaaccg ttgaaggcgt
    10201 aaacgatgcc aaattcgact tcaaacgcgt gattgaggaa acgtttgaag tgtttaaaaa
    10261 tttggggctg gagggagaaa aaatcccgct tattcttgcg ggaggcatgg caaattttga
    10321 aaaagtcaaa accgccctaa agaactgggg agcctccgcc gttcaaatcg gtacggcttt
    10381 tgccgttacc gaagaaggag atgcacacct caactttaaa aaaacgctcg ccagtgcgga
    10441 aactgaaaaa gtagtcgaat ttatgtctgt tgccggtttg ccggcgcgcg gtatccgcac
    10501 caaattccta gacagctata tcaggcgcga aggcaaactt caggcaaacg ccaaagccga
    10561 cccgcgccgc tgtacccaag gtttaaactg cctgaccagc tgcggtctgc gcgacgggct
    10621 ttccaaagca gggcaattct gtattgatat ccagcttgct gccgcattcc gtggagaagt
    10681 tgataagggg ttgtttttca ggggaaaaga ccagcttccc ttcggtaatg ccatccgcac
    10741 cgtccgcgaa acgatacaat atctgctgac ggggagcgaa cctgttgcaa cgctcggacg
    10801 ctgacatcag gtaggatttg atttgcaaac aaaatgccgt ccgaagggct ttcagacggc
    10861 gtttttcagg ttgtgttccg gaacagtgtc gaaaaaacgg agagaaataa aaacgttttt
    10921 atgtgaacaa aatgccttcc gcattatgtc ggacatacaa cacaaatgcc gtctgaagcc
    10981 tttcagacgg catttttcag gctgcctttc ggaatgatgt taaaaaataa acgggatgag
    11041 atacatttat tttcgtccga caaatcagac catcacgccg aataatcaaa caatgcctgc
    11101 ccgacattgc atataacaaa ccaatgcaat gaaaacacgg cttttttatt tgcttcctgc
    11161 attattgaaa agtttgtcca tcggggtcaa atcgaccgca ttgccctggc tggtaatcca
    11221 tttgttctcg acgcgccaca cgcctgccgc gtcctccacg cgcacatacc agtggttggc
    11281 cggcggaagg gttttgaaca ccgcctcata ttccgccctg ccgttctgcg cgctgccgac
    11341 gggcttgagg gcgacggttt gatcgtccgc cttgcgggtc gggtgcatca gcagcaggtt
    11401 caaaggctgt ttgccgtcaa actcgccgcc gacaaacact tttgccgcat tcatatcggg
    11461 agaaatgagg acctgcaccc cgatatgccg tctgacggct tcttcatccc gatgaagctg
    11521 gatgtcgata tgcttgccat ccttataata atcgtccgta accaaatctg tcgcgtgctg
    11581 ctgcgcgaca aaaaacatag cgacgctggc gatgacgaca aaaatcggcc ccgccatcaa
    11641 gacccacggc cagacgtgtt tgtaccaagg cttgattgga ttgttttgag acacggttat
    11701 tctccgataa aggttgcgtc ttcttccaaa acaaccggct tgccgtctga tgcaccgctt
    11761 tcccggtatt ggaagataaa ctcgatagga tgactgcctt tgtccgcgta ttccggaatg
    11821 gtggacactt ggacgggaag ggttattgtt tcgcgcggag ccacttttat accgccttca
    11881 ggcagcccgg tcagggcgat ttcgtcaaat cctttgacac ttgcggtaat cagctgttct
    11941 ttttcgctgt tgttgatgat acgcaggcta tatgcgtttt ccagccaacc tttggcgttt
    12001 tcgcgcacca ttacgccacg gtctttcaga atatcgactt cgaccatttt gcgcgtggac
    12061 aaaccgacca ggaaggcggc gacaaccact gccaacaccg cgccgtaacc tgccacgcgc
    12121 ggtctgagca gccgtttttt aatgtctttt tcggcatatt cgtgttccag cgcgctttcg
    12181 gtcgtatagc gtatcaaacc gctcggataa cccattttgt ccataatctc atcgcacgcg
    12241 tcgatacagg cggcgcagcc gatacattgg tattgcaggc cgttgcggat gtcgatgccg
    12301 acggggcaga cttggacgca catcgcacag ttgatgcagt cgcccaaacc cgcctcttcc
    12361 ttgttgaccg ttttcttgcg ggcgccgcgc ggttcgccgc gctccgcgtc ataagaaaca
    12421 atcagcgtgt ccttatcgaa catcgcgctt tggaaacgtg cgtacggaca catatgcagg
    12481 cacacttttt cgcgcataat atgggcgaag aagaaggtca taaagccgta aaacgctgcg
    12541 gcaaacatcg cgccgccgcc tgctgctccg gtgaataaat cgggaacaaa ctggcggatg
    12601 gggacaaacc agccggcaaa cgtgatgccc gtccacgcgc agacaaggaa aatcaacagg
    12661 tatttggtgg ctttgatgcg gattttggtg aaattccacg gcgatttttc cagcttcagg
    12721 cgtttgtttc tatcgccttc gaccaggttg tcgatccaca gcataatttc ggtgtaaacc
    12781 gtttgcgggc aggaatagcc gcaccacaaa cggccggcaa tcgtcgtcca ccaaaacagc
    12841 ccgaaggcgc aaatcatcag cagcagggca aggtaaatca aatcgcccac ccccaacgac
    12901 aatccgaaaa tgaagaaatg ccgttcgggg atattgaaaa cgacggcctg ccttccgctc
    12961 caattgaacc acggaatgac gtaaaacaca aactgggtcg ccaatacggc ggcgatacgc
    13021 agtttggcga accgtccttc cgcctttttg ggatggatgc gttcgccttc gggatggatt
    13081 tgaatcacgc tggcacgcgg atcgaacgtt tttttcactt tcggcgcggc ttttgtttgt
    13141 tcggacgtgc cgattccgga tgccggactg ccggcttggt tttccgtggt cattctgcat
    13201 tccttagatt tttgattgat ggtttgcccg ttaccgccgc cgttttgctt ttcagacgtc
    13261 atttttcttg tttttttaag gcattgtgtt tcaagttttg agaaaatccg cttttcccaa
    13321 aatatattcc cgctattgta caactttatg tgccgtccgg atgtattggg cgaatacatt
    13381 tcccatccgc atcaaaacgc cctgatttta ccgcaccgcc cgaacaaaat taaaatactc
    13441 ttaaaaaaca aagactaaaa aaacagacac ccccttatcg gcagaaccgc cggcgcaagc
    13501 tcataaacaa acgctatcaa caatccggca cacaatctat aacgttttat ttcaaaagga
    13561 ata
//
LOCUS       DBJGGI010000056        13120 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.056, whole genome shotgun sequence.
ACCESSION   DBJGGI010000056 DBJGGI010000000
VERSION     DBJGGI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13120
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.056"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..1251
                     /locus_tag="ACSIGJ_001607"
     CDS             <1..1251
                     /locus_tag="ACSIGJ_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HHQ1330435.1"
                     /translation="KYAVALGSSSTASGEYSYASGYNSVASGNKSYAAGYASVASAEG
                     SVVIGDSRQVKPEADQGVAVGSKATVKNKAKQRVVVGSEAKVNAERGIAIGKEAKAGG
                     KTTNTLLDGPAYYADAIAVGYQAEAGKGGAIALGKQAKATKQNGMALGVESEAAGDFS
                     TAVGNESKAKGQGGVGLGNQSKAEADFAVAVGNKAEATKENSLVIGRYARANGNHSVS
                     LGSRSEIKDGVSNSVAPGYGSVASENNVVSVAYKETPQSTELSYRKIVGVDDGVNDFD
                     AVNVRQLKAMQGQNMAELFSVRSEVRGVAASSAALSALTPLSYDANNPTQFMVGFGTY
                     KGRQAMALGLSHFVNDRFMVKVGGTLGGNKTGHMMNVGLTWKFGTSQGVPATSDKVLM
                     EQLMQDNRQLKARLEKLEARLNAM"
     gene            complement(1390..1905)
                     /gene="coaD"
                     /locus_tag="ACSIGJ_001608"
     CDS             complement(1390..1905)
                     /gene="coaD"
                     /locus_tag="ACSIGJ_001608"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HHQ1330436.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRNTYTAAERQDMLCAITDNFPNVRIEVFQNRFLVHYAREVDAGFIVRGIRSTSDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMIKGLVGPEGWMETVKRYVPPAVYQ
                     KMIAEHHNNNA"
     gene            complement(2035..2718)
                     /locus_tag="ACSIGJ_001609"
     CDS             complement(2035..2718)
                     /locus_tag="ACSIGJ_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HHQ1330437.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSYISAALTLFISLYNIFSSLLN
                     ILLSLNGDD"
     gene            complement(2875..3141)
                     /locus_tag="ACSIGJ_001610"
     CDS             complement(2875..3141)
                     /locus_tag="ACSIGJ_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HHQ1330438.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            complement(3215..3685)
                     /gene="rlmH"
                     /locus_tag="ACSIGJ_001611"
     CDS             complement(3215..3685)
                     /gene="rlmH"
                     /locus_tag="ACSIGJ_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HHQ1330439.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDAAYALKEIKPEKRG
                     AGVNAVQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAVSILHNHPYHRE"
     gene            complement(3738..4124)
                     /gene="rsfS"
                     /locus_tag="ACSIGJ_001612"
     CDS             complement(3738..4124)
                     /gene="rsfS"
                     /locus_tag="ACSIGJ_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HHQ1330440.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTIWGGEKPSFHAGMQKPWHAAD"
     gene            complement(4183..4788)
                     /gene="nadD"
                     /locus_tag="ACSIGJ_001613"
     CDS             complement(4183..4788)
                     /gene="nadD"
                     /locus_tag="ACSIGJ_001613"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HHQ1330441.1"
                     /translation="MKKIGLFGGTFDPIHNGHFHIARAFADEIGLDAVVFLPAGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVRESATYTFDTVQIFRRQFPSAQLWW
                     LMGSDSLLKLHTWKKWQLLVRETNIAVAMRQGDSLHQTPRELHAWLGNALQDGSVRIL
                     SAPMHNVSSTEIRRNLSAAGVSDGIPPVAARYIRKHGLYEK"
     gene            complement(4785..5384)
                     /locus_tag="ACSIGJ_001614"
     CDS             complement(4785..5384)
                     /locus_tag="ACSIGJ_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HHQ1330442.1"
                     /translation="MSELSEILSYNQKFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGVDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            complement(5413..6954)
                     /locus_tag="ACSIGJ_001615"
     CDS             complement(5413..6954)
                     /locus_tag="ACSIGJ_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHQ1330443.1"
                     /translation="MDGRRWAVRGAFSLLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMFKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFAQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAGASALVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFSVAVLSVCCLFPLSAIVVKAWSAGESRRVLMESETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRLVWMRGLVFLPFMVSPVCVSAGVLLLYPGWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFAVCIFLLLDNGEGGK
                     RTETL"
     gene            7345..8731
                     /locus_tag="ACSIGJ_001616"
                     /pseudo
     CDS             7345..8731
                     /locus_tag="ACSIGJ_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
     gene            8751..9269
                     /locus_tag="ACSIGJ_001617"
     CDS             8751..9269
                     /locus_tag="ACSIGJ_001617"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HHQ1330444.1"
                     /translation="MTTHFVVTGVCGCGKTTAAPSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGADHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(9455..10372)
                     /gene="thrB"
                     /locus_tag="ACSIGJ_001618"
     CDS             complement(9455..10372)
                     /gene="thrB"
                     /locus_tag="ACSIGJ_001618"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HHQ1330445.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELSFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAGEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(10409..11137)
                     /gene="ubiG"
                     /locus_tag="ACSIGJ_001619"
     CDS             complement(10409..11137)
                     /gene="ubiG"
                     /locus_tag="ACSIGJ_001619"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HHQ1330446.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            11289..12530
                     /locus_tag="ACSIGJ_001620"
     CDS             11289..12530
                     /locus_tag="ACSIGJ_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter"
                     /protein_id="HHQ1330447.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGIGSAVGGKISLTVGQLVFFGILAFCVWASARLVDRFTGVLIGGMVLTF
                     IWATGGLVADAKPSVLFDTQAPAGTGYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWAGTLVALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSMSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGCVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIANIAAQVLSQMELVPVFKG"
     gene            12538..>13120
                     /locus_tag="ACSIGJ_001621"
     CDS             12538..>13120
                     /locus_tag="ACSIGJ_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HHQ1330448.1"
                     /translation="MPFETRRRLQAALPQQTATVFRHTAHYPTPSRTNPAPTGAGRNH
                     KGTMMKLKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFDHGIPEYAMIE
                     PHLVDQRRTLKKLRSFLFSSRLAQTRKNSAAKLIACHHADYADLLICGGTHLGYLHHM
                     AQKPNLLDRLAIRRNRSNYATAKLIVAHSHMMRR"
BASE COUNT         3094 a         3840 c         3407 g         2779 t
ORIGIN      
        1 aaatatgcag tagcattagg atctagttct actgccagtg gagagtattc ctatgcgtcg
       61 ggctataatt ctgttgccag tggaaataaa tcctatgctg caggctatgc ttctgttgcc
      121 agtgcagaag gcagtgttgt aattggcgat tcaaggcaag taaaaccgga agcagatcaa
      181 ggggttgcag taggttctaa agcaacggta aagaacaaag ctaaacaaag ggttgtggta
      241 ggttctgagg caaaggtaaa tgcagaacgt ggtattgcga ttggtaaaga agccaaagca
      301 gggggcaaaa caacgaacac actgttagat ggaccggcat attatgcgga cgctattgcc
      361 gtcgggtatc aagcagaggc tggcaaaggt ggcgccatcg ctttgggaaa acaagcaaaa
      421 gcaacgaagc aaaatggaat ggcacttggc gtagaatcag aagctgccgg tgattttagt
      481 accgccgtgg gtaatgaaag caaggcaaag gggcaaggcg gcgtaggtct cggtaaccaa
      541 tctaaggcag aggcagattt tgcggtggct gtggggaata aggctgaagc gactaaggaa
      601 aactcacttg tcattggccg atatgcaagg gcaaacggaa atcattctgt gagtttagga
      661 tctcgtagtg aaataaaaga tggtgtgagc aattctgtgg caccgggtta tggttcagta
      721 gctagtgaga ataatgtcgt ctctgttgcc tataaagaaa caccacaatc tacagaactt
      781 tcctatagaa aaattgtagg tgttgatgat ggtgtcaatg attttgatgc cgtcaatgta
      841 cgccagttga aagcgatgca aggacaaaat atggctgagc tattttcagt acgcagcgaa
      901 gtgcgtggtg tggcagcgag tagtgcagcc ctatcggctc tgactccatt atcctatgat
      961 gcaaacaatc ctacgcagtt tatggtcggt ttcggcacat acaaaggacg tcaagctatg
     1021 gcattaggat tgtcccattt cgtcaatgac cgctttatgg taaaagtagg cggaacctta
     1081 ggcggcaata agacgggaca tatgatgaat gtaggcttga cttggaaatt tggaacgagc
     1141 caaggtgttc cggctacaag tgataaggtg cttatggaac agttgatgca agataatcgt
     1201 caattaaagg cacgattgga gaaattagaa gcacgtctga atgctatgta gccttgtggc
     1261 gataacggtt tattgagtat atcgccaatg ataaagtgca taattttcaa accgttgata
     1321 atatacaata aattgtaacc aatacctcaa acgggtttga acccgaatat gcctgacagg
     1381 aactttctgt caggcattgt tgttgtgatg ttctgcaatc attttttggt acacggccgg
     1441 cggcacatat cttttgacgg tttccatcca gccctcgggc ccgaccagtc ctttgatcat
     1501 agtggacgac acttcggcga tttcgcgcgg cggcatgagg aatacggtag atatttcggg
     1561 tgcgaggtcg ctgttgatat ggcgcatgga gcgttcgtat tcataatcgg aggtggaacg
     1621 gatgccgcgc acgatgaatc ccgcatctac ctcacgggcg taatgcacca aaaaccggtt
     1681 ttgaaacact tcaattctga cgtttgggaa gttgtcggta atggcgcaca acatatcctg
     1741 cctttcagcg gcggtatagg tgttgcgttt gtcgggattg atgccgatgg cgacgatgag
     1801 ttcgtcaaac atagattgcg cctgccgtat catccacaga tgccccaatg tgggcggatc
     1861 gaaactgccg gcataaacgg cgcggcgcgg tgtattcggt aacatttgat tcctcccggc
     1921 ttcatagccg gctgtgtttt tatgtgtttg catccgtatt gtatgcccaa aacaaaatgc
     1981 cgtctgaaaa taagtgcttt cagacggcat ggtttgcatg gtgtttcgcc tacatcagtc
     2041 gtcgccgttt aaggacagca ggatgttgag caggctgctg aagatgttgt aaagcgagat
     2101 aaacagtgtc agtgccgcgc tgatgtaact gtcttcgccg ccgtcgatga cggtgcgtac
     2161 ctgccacata atcattaagg aactgaacaa gacaaaaccg gcggaaatgg tcagggcgag
     2221 tgcgggaata cccaaaaaca gattggcaac cacggcgacc atcagaatga ccgcacctac
     2281 ggtcaggaag cgtccgagcg cgttcatatc gagccgggtt cggcgtgcca atgcggacat
     2341 cgttaaaaag acggcggcgg tcatcgcggc ggcaatgccg acgattttcg caccgtcggc
     2401 aatatggagt gcgtattgca gcacggggcc gatcaatacg cccataccga atgtgaatac
     2461 catcagcagg gtaacgccgg tattgctgta acggtttttt tcgatgaagt ggatcatacc
     2521 gtagaaaaac gccaatacga cggcaaatcc tatccagcgc gaaccgaagg cggcgtaaaa
     2581 attgaaaccg gcattggcgg caagtgccgc gcctgcggct gccggaatga atgaaaatcc
     2641 gagcaggcgg taggttttct gcaggacggt gtttttagaa accgtatgcg cggtgtagtc
     2701 gtaaacgtcg tgttgcatat catctgctcc tgaaagcgcg gttggaaata acgggggatt
     2761 ttaacattgc ccaatgtcaa aatttgtccg gttgcgtgaa gataaagttg tccggcgtat
     2821 tttaaaggcc gtctgaagca gtttcggaca gcctgtgttc aaaacggaaa accgttattg
     2881 cggaacgtat ccctgcacgg catccgcgcc gtcgccgaag aaatactgct ccatctgctg
     2941 agccaggtat tcgcgcgcgc gcggatcggc aaggcttaag cggttttcgt taatcagcat
     3001 ggtttggtgg cgcgtccacg ccgcccatgc ttcttgcgat acgttttcaa aaatgcgttt
     3061 gcccaattcg ttgggcagcg gcggaaattt catgccttcg gcttctttgt tgagcttgac
     3121 gcagaatacc attcgtgcca tagcggattc ctttgctgtg ttcagaaata acggggcgat
     3181 tttaaccgat tagggggtac agacaaaagc ctttttattc ccgatgataa ggatggttgt
     3241 gcaggatgga aacggcccgg tagagctgct cggtcagaag gacgcgcacc atgccgtgcg
     3301 gcagggtgag gctggacagg cgcatcatca tgcgggcttg ttgtttgagg cggtcggtca
     3361 tgccgtccgc gccgccgatg acgaagcaga cgtgttcgcc gttttgccgc cagcttttga
     3421 ggtgttccgc cagctcgacg gaggtcggtg ctttgccgcg ttcgtcaaga acgacgagga
     3481 acgcgccttg cggaatggct tcaaggatgc gtttttcttc cgccgccata ccttggacgg
     3541 cattcacgcc cgcgccgcgt ttttcgggtt tgatttcttt gagtgcgtag gcggcgtcgc
     3601 gtccgaagcg tttggcgtat tcggcgacgg cctcatcaac ccaacgcggc attttggtgc
     3661 cgactgccaa gacggtaatg ttcaatgctt tcccccttac aggaaatgcc gtatgaatat
     3721 tcagacggca tcgggaatca gtctgcagcg tgccacggct tctgcattcc ggcgtggaaa
     3781 ctcggtttct cgccgcccca gatggtgtca atgtcgtaga agtcgcgcac ggcagggagc
     3841 atgacgtgga cgacgaggtc tcctgcatca accaacgtcc attcgccgct gtctccttcg
     3901 gtactgagga tttcaaaacc ggcttctttc aaatcgacgg caacgttgtt ggccagtgct
     3961 ttgacttggc gcgtactgtc gccgctggcg ataatcattc tggcaaacag cgaagttttg
     4021 tcttgcgttt cgagaacgga aatgtctttg gctttgatgt cttcgagggc attgacggcg
     4081 accccgacca ttttttgcag gtcttgcagt tcttgttcgt tcattgtttt cctaacggga
     4141 tgttttcaga cggcattata gccgttcctt actgatttga ctttattttt catacaaacc
     4201 gtgttttcgg atataacggg cggcgacagg cgggatgccg tctgaaacac cggcggcact
     4261 caggttgcgg cggatttccg ttgacgacac attatgcatc ggggctgaca agatgcggac
     4321 gctgccgtcc tgaagggcat tgcccagcca cgcgtgcagt tcgcgcgggg tttggtgcag
     4381 gctgtcgccc tgccgcatgg cgacggcgat attggtttcg cgcacgagca actgccattt
     4441 tttccaagta tgcagcttca gcaggctgtc gctgcccatc agccaccaaa gttgcgcgga
     4501 cgggaactgc cggcggaaga tttggacggt atcaaaagta taggttgcac tttctcggac
     4561 gatgtcgcag tcgctgacgg caaaacgcgc gtcttctgcc gtcgccagtt cgaccatggc
     4621 aaggcggtcg gcggcggaag cggaggcggc gtctttgtga tacgggccgc ctgccggcag
     4681 gaaaacaacc gcgtccaacc cgatttcgtc ggcaaaggca cgggcgatat gaaaatgccc
     4741 gttgtgtatc gggtcgaaag tgccgccgaa cagtccgatt ttcttcatga tgtttccatt
     4801 ccttccgata agtccatgcc gtctgaaaca ctgccgtcca caaccagcgt cagtttgccg
     4861 gtaacgggat ggatgaccag tccgtgaacg gcgatatggc gcggcatcag cggatggttg
     4921 cggataaggt ccaccgtgtg gcgcacgctg tcttcgacgt tgtcgaaacc ggtcagccag
     4981 ccgtcgaggt cgacacctgc ataacgcagg gtttcgatac ggtcttcggg aatccggctt
     5041 tcccggacgc gcccgaggaa ttcttcggca ttcagcccct gcataccgca atcgtgatgg
     5101 gcgatgacca taatctctct gaccttcaat tcaaacacgg caaccaaaag gctccgcatc
     5161 accgaacccc acgggtgcgt aaccagcgcg ccggcatttt taatcagctt ggcatcgccg
     5221 tttttcaaac ccaacgcgtc gggcagcagc ccgataatcc gcgcatccat acaggacaaa
     5281 accgccagcc cgcgttcggg gtatttgtcg gtaaagtatt tttcatattc tcccgactcg
     5341 acaaacttct gattatagga aaggatttcg ctcaactcgc tcattttgcc gtcctctgaa
     5401 aaagggttcg cattataacg tttccgtccg ttttccgcct tcgccgttgt ccaacagcag
     5461 gaaaatgcac accgcaaatg ccgacaacag caatgtcaac accattgccc gcgcataatt
     5521 gtcctcaccc gcacgcccca aataggcata aatcaaagtc gtcaacgtct gccattccgg
     5581 acgcgacagg aacaaggttg ccgcaaattc gcccacacac gtcgccgccg ccaaagtcag
     5641 accgcgccgc aacgccggtt tcaagagggg gaacgtgata cggcatgccg tctgaaagcc
     5701 gtttgcgccc aaacctgccg ccgccctgcc gtaatccggc ggcagtgcat cccaggccga
     5761 taaaacatct tttgccacaa acggatacgc cagcagcgca tacatcgcca gcagcagcgg
     5821 taacgaagcc gtccaccccg gataaagcag cagcacgccc gccgaaacac aaaccggcga
     5881 caccataaac ggtaaaaaca ccagtccgcg catccacacc agccgccgcg ccgccgccgc
     5941 atacaccaca cccaaaaccg ccgccgcaaa caccgccgcc gccgaaaagc gcaaagtatt
     6001 ccacactgcc tgccacgttt cactttccat taacacacgc cgcgattcgc cggccgacca
     6061 cgctttcaca acaattgccg acaaaggaaa caggcagcac acggacaaca ccgccaccga
     6121 aaatgccagc aatacatatt cccccaccga ttgaggcggc gacggcatca cgggggaaac
     6181 cgccttatcc gaaaccgcgc gcctgccgaa ccacgcatac agcaaccctg ccgccgccgt
     6241 tacccccaac accagccaca ccagcgccga agcccccgcc atatcgagtt cgaacataac
     6301 caactggtaa atttccactt cgaccgtggc ataacggctg ccgcccaaca gcaatgccag
     6361 cccgaacccc gaaaaacaat acaggaagac aaggcacacg ccgccggcaa gccacgggcg
     6421 caaaacgggc atttcaatgt cccaaaaccg ccgccacgcc cccgcgccca acgtccgtgc
     6481 cgtctgaagc cgtgccgcag gcacttgagc aaacccctga tacgccgccc tgaccaacac
     6541 gggcaggtta aaaaacacat tgccgtacaa caacagatac ggcgtatcct gccggccgcg
     6601 ccacaacagc ccgtccgccc cgaacagagc cagcacgccc acgcccgcca ccagcgtcgg
     6661 catcacaaac ggcagcatca gcaggcgcag caccaaagcc cgccccggga acgccagccg
     6721 cgccagcacc cacgcgacag gcacgcccaa aggcagcacc agcacacagg ttgccgccgc
     6781 ctgaaacacc gtccacgcca aacgtttgaa catataggca tccgacagca ccgcgcgcca
     6841 cgccaaaccg tcatacgccg ccaccgccca caaaggcgca acgaccatta ccgccaaaaa
     6901 agccgaaggc agcagggaaa aagcaccccg taccgcccaa cgccgtccat ccatcgcctt
     6961 ccccacttga aacactgatg ttgcgattgt acccaaaagc cccacatacc gtatatttca
     7021 atccgactac gtaccgtatc cgccttcctc ccgccgtctg aaatatagcg gattaacaaa
     7081 aatcaggaca aggcggcggg ccgcaggcag tacaaatggt acggaaccgg ttcgcccggt
     7141 gcttcagcgc cttagggaac cgttcccttt gagccggggc ggggcaacgc cgtaccggtt
     7201 tttgttaatc cactataaat cgttcaaata aacaggaata taacttcaga caaacaactt
     7261 accgcccgat ttgtgctatc gttttcgcac aacttaaaaa aacctgacaa ttttgtactt
     7321 ttattacaga gaaaggcttt acaaatggac ggccggacac agacgctgtc cgcgcaaacc
     7381 ttgttgggca tttcggcggc ggcaatcatc ctcattctga ttttaatcgt caaattccgc
     7441 atccgcgcgc tgctgacact ggtcatcgcc agcctgctga cggctttggc aaccggtttg
     7501 cccacaggca gcatcgtcaa cgacgtactg gtcaaaaact tcggcggcac gctcggcggc
     7561 gtggcgcttc tggtcggtct gggcgcaatg ctcggacgtt tggtagaaac atccggcggc
     7621 gcacagtcgc tggcggacgc gctgatccgg atgttcggcg aaaaacgcgc accgttcgcg
     7681 ccgggcgttg cctcgctgat tttcggcttc ccgattttct tcgatgccgg actaatcgtc
     7741 atgctgccca tcgtattcgc caccgcacgg cgcatgaaac aggacgtact gcccttcgcg
     7801 cttgcctccg tcggcgcatt tatccgtcat gcacgtcttc ctgccgcccc atccgggccc
     7861 gattgccgct tccgaatttt acggcgcgaa catcggccag gttttgattt tgggtctgcc
     7921 gaccgccttc atcacatggt atttcagcgg ctatatgctc ggcaaagtgt tggggcgcgc
     7981 catccatgtt cccgttcccg aactgctcag cggcggcacg cgagacagcg acccgccgaa
     8041 agaacctgcc aaagcaggaa cggtcgtcgc cgtcatgctg attcccatgc tgctgatttt
     8101 cctgaatacc ggcgtatcgg ccctcatcag cgaaaaactc gtgagcgcgg acgaaacctg
     8161 ggttcagacg gcaaaaatga tcggttcgac acctgtcgcc cttctgattt ccgtattggc
     8221 cgcactgttg gtcttgggac gcaaacgcgg cgaaagcggc agcacgttgg aaaaaaccgt
     8281 ggacggcgca ctcgcccccg tctgttccgt gattctgatt accggcgcgg gcggtatgtt
     8341 cggcggcgtt ttgcgcgctt ccggcatcgg caaggcactc gccgacagca tggcggattt
     8401 gggcattccc gtccttttgg gctgcttcct tgccgccttg gcactgcgta tcgcgcaagg
     8461 ttcggcaacc gtcgccctga ccaccgccgc cgcgctgatg gctcctgccg ttgccgccgc
     8521 cggctttacc gactggcagc tcgcctgtat cgtattggca acggcggcag gttcggtcgg
     8581 ttgcagccac ttcaacgact ccggcttctg gctggtcggc cgcctcttgg atatggacgt
     8641 accgaccacg ctgaaaacct ggacggccaa ccaaaccctc atcgcactca tcggctttgc
     8701 cttgtccgca ctgctgtttg ccatcgtctg acagacggaa aggatagtaa atgactacgc
     8761 attttgtcgt tacgggcgta tgcggctgcg gcaagaccac cgccgcgccg tccctgcaga
     8821 aacacctcgg tcaatgtccc tatgccgaag gcgacgagtt ccacacccaa gccaaccgcg
     8881 acaagatggg cgcgggtatt ccgctgaccg atgaagaccg ctatccgtgg ttgggcaatc
     8941 tgcgcgactg gatgacgcaa caggcgcaaa acggcgcgga ccacaccatc gtaacctgtt
     9001 ccgccctcaa acgcggctac cgcgacattc tgcgcggagc cgaaggcaaa gccgccttca
     9061 tccacctcag tccgccgcaa gacatcaacc tcgaacgcat gatgtcgcgc aaagggcact
     9121 acatgaaagc agggatgctc gattcgcaac tggaaatcct cgaggaactg ggcgaaggcg
     9181 aatacggggt caaaatcgcc aaccccggca cgcccgaagc ggtcgaagcc gatattctga
     9241 actgggttgc ctcggaaaac ctgctttgaa gcaatatgcc gtctgaagcc cggcacagga
     9301 tgggtttcag acggcataaa catcgggaac agaatgaatt acattgattt aaaatggaaa
     9361 atacccggct atcgtctcat tttcgtttta atcagccata aaaatgccgt ccgaaaccct
     9421 ttcagacggc atttctgtca aacgccggac gcactcaatc caaactcaac agcaggttgc
     9481 ggaacgcgtt cgggtctttg ataaacgtca tctcgcccgc ctgcggaaaa tgaaaatcca
     9541 acaggcgcga cacccaaaaa cggatgcagc cggcgcgttg ggcggtcggg aaatacgcct
     9601 tttccccggc actcaagggg cgcacgccct cataaccgcc gataaacgcc tttttcaacg
     9661 cctcatccaa cttattgtcc gccgtccttg cccaatcgtt gaccgcaatc gccaagtcat
     9721 acataaaatt gccccggcag gcataataga aatcgatgaa gcccgatacc tgaccgccgt
     9781 caagcaacac attgtcttta aacaggtcgg cgtggatgat gcccgaaggc agatggttgc
     9841 cgagattgtc cttcagcgca tcgatttcgg aacacagcag cgcggcatcg tcttgcgaca
     9901 ggacgggcag cagccgggcg cacgcttccg tccaccacgc atcgtaacgc gggttttcca
     9961 tttccaaagg gaaatcggcg gcggcaaggt gcattttcgc caacatcgca ccggtatgga
    10021 aacactgctc agccgtcggc agcgcggtat ccgaaccttt caggcaggca accaggcagg
    10081 ccggcttgcc cgccaaaacg gaatcgagcc ggccgtcttt gcgcgcaacc ggcgcggcaa
    10141 ccgccacgcc cttcatactc aaatgccggt taagctccag aaaaaacgac agctcttcct
    10201 gtttcaacac ttcaaacacg gtcagcacat aacgtcccga agtcgtcgtc agaaaataat
    10261 tgctgttggt aatcccctgc gcaatgccct gcagggaaac aaattccccc aaatcgtaac
    10321 cgctcaggaa gccgcgcatt tcatcatcgg aaacactggt atagacagac atatcaactt
    10381 acttgctcaa aaacgggcaa acccgccgtc agaacgccgg acggcaggca aacatataat
    10441 tcacatcggt cgaatcgcac agggcataag tttgcgacaa cacatggtaa gtcataccct
    10501 tcgtatccgc cacatccaag cccgcctgac ggcacatccg cgccagctcg gcaggcgcga
    10561 tgaatttttt ccagtcgtgc gtgcctttgg ggacgaactt caacagatat tccgccgcca
    10621 caatcagatg caggtacgat ttcgggtttt tattgatggt ggaaaaaaac accatgccgt
    10681 ccggcttgac cagcttggca caagcacgca cgatggcggc gggatcgggg acgtgttcca
    10741 tcatttccat gcacgttacc acatcgaacg agtgcggttc cgcctcggca aggtcttcca
    10801 cgcggataca ttcgtattcg atatcggcga cattgttcaa agccgcgtgc aggcgggcgg
    10861 tttccagcga ctgctccgcc atgtcgatgc ccttgacaaa cgccgcgccg cgccgcgcca
    10921 tgctttccgc aaggatgccg cctccgcagc ccacgtccaa aacccgtttg ccgcacaaat
    10981 ccgcgtgtcc gtcgatataa tcgagccgga gcggattgat gtcgtgcaag gttttgaact
    11041 cgcccgactt gtcccaccat ttgtcggcaa tccggctgaa tttggcgatt tccccctcat
    11101 cgacattata ttttttgtcg gacattttcc ctcccatctg acgaaccgcc cactccaaaa
    11161 cccaagatta tactatggaa cacaatgccg tctgaaagcc ttttcgggcg gcgcggcgca
    11221 aatgccttac aaatccttac actttacggc ataatggcgg ctcgcttttt ctggcagaaa
    11281 gacaaaatat gcccaccaaa accccttcac tgttcggcgg cgcgatgatt atcgccggca
    11341 cggtcatcgg cgcaggtatg ctcgccaacc cgaccgccac atccggcgta tggtttaccg
    11401 gctcgctggc cgtgttgctg tacacctggt tttctatgct ttccagcggc ctgatgattt
    11461 tggaagtcaa cacccattat ccgcacggcg caagtttcga cacgatggtc aaagacctgc
    11521 tcggacgcgg ctggaacatc atcaacggca tcgccgtcgc cttcgtttta tacctgctca
    11581 cctacgccta catcttcgtc ggcggcgacc tgaccgccaa aggcatcggc agcgcagtag
    11641 gcggcaaaat ttcgctcacc gtcggacaac tcgtcttctt cggcatcctc gccttttgcg
    11701 tatgggcatc cgcacgcttg gtcgaccgct ttaccggcgt cctcatcggc ggcatggtat
    11761 taacctttat ttgggcaacc ggcggcctgg ttgccgatgc caaaccgtcc gtcctcttcg
    11821 acacccaagc ccccgccggc accggctact ggatttacgc cgccaccgcc ctgcccgtct
    11881 gcctcgcttc cttcggcttc cacggcaacg tttccagcct gctcaaatac tttaaaggcg
    11941 acgcgcccaa agtggcgaaa tccatctggg caggtacgtt ggttgccttg gtaatttacg
    12001 tcctctggca aaccgccatc caaggcaacc tgccgcgcaa cgagttcgcc cccgtgattg
    12061 ccgccgaagg gcaagtctcc gtcctgattg aaaccctgtc caaattcgcc caaaccggta
    12121 atatggataa aatattgtcc ctattttcct acatggcaat cgccacctcg tttttaggcg
    12181 taaccttagg cctgttcgac tacatcgccg acatcttcaa atggaacgac agcatgtccg
    12241 gccgcaccaa aaccgccgcg ctgaccttcc tgccgcccct gatttcctgc ctgctcttcc
    12301 ccaccggctt cgttaccgcc atcggctgcg tcggcctggc ggcaaccgtc tggacaggca
    12361 tcatccccgc catgctgctc taccgttcgc gcaaaaaatt cggcgcaggc aaaacctata
    12421 aagtttacgg cggcttgtgg ctgatggttt gggtcttcct tttcggcatc gccaacatcg
    12481 ccgcacaggt attgagccaa atggaactcg tccccgtatt taaaggataa aggcaaaatg
    12541 ccgtttgaaa cccgccggcg gcttcaggcg gcattgccgc aacaaacggc aaccgtattc
    12601 cggcacacag cgcattaccc taccccctca cgcacaaatc ccgccccgac aggcgcggga
    12661 cgcaaccata aaggaacaat gatgaagctc aaaatagaca ttgcaaccaa caacttcaaa
    12721 cacggcggcg gcacggaacg ctacacattg gatttggtaa agggtctgaa cagacaaaac
    12781 atcacaccgg ccgtttatgc gacgaaattt gatcacggca ttcccgaata cgccatgatc
    12841 gaaccccatc ttgtcgatca acgccggacg ctgaaaaaac tacgctcgtt cctcttttca
    12901 agccggctcg ctcaaaccag aaaaaacagt gccgccaaac tgattgcctg ccaccacgcc
    12961 gattacgccg acctcctcat ctgcggcggc acgcacttgg gctacctgca ccatatggcg
    13021 caaaaaccga acctgctcga ccgcctcgcc atacgccgca accgcagcaa ctacgccacc
    13081 gccaaactga ttgtggcgca ttcccatatg atgcggcgcg
//
LOCUS       DBJGGI010000057        13066 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.057, whole genome shotgun sequence.
ACCESSION   DBJGGI010000057 DBJGGI010000000
VERSION     DBJGGI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13066
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.057"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(167..361)
                     /locus_tag="ACSIGJ_001622"
     CDS             complement(167..361)
                     /locus_tag="ACSIGJ_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HHQ1330449.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMNK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(351..2432)
                     /locus_tag="ACSIGJ_001623"
     CDS             complement(351..2432)
                     /locus_tag="ACSIGJ_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HHQ1330450.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMTVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRHIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLNFPVDAATLLHTAKEVGENTILSRTGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVALVPAVGVLLVTCYAGLQKLFHNDPRASFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVTVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEEQSEA"
     gene            2955..3335
                     /locus_tag="ACSIGJ_001624"
     CDS             2955..3335
                     /locus_tag="ACSIGJ_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HHQ1330451.1"
                     /translation="MNIRFFALTVSVLSLAACAVPEAYDGGGRGYMPPVQNQAGPDDF
                     RAFSCENGLSVRVRNLDGGKIALRLDGRRAVLSSDVAASGERYTAEHGLFGNGTEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3486..3890)
                     /locus_tag="ACSIGJ_001625"
                     /pseudo
     CDS             complement(3486..3890)
                     /locus_tag="ACSIGJ_001625"
                     /inference="COORDINATES: protein motif:HMM:NF018915.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
     gene            4291..5679
                     /locus_tag="ACSIGJ_001626"
     CDS             4291..5679
                     /locus_tag="ACSIGJ_001626"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HHQ1330452.1"
                     /translation="MNEYSQLIKHPDISLSPISDGIGVGNPATGEILAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIVKP
                     ASLTPLSAYALAVLAYEAGVPQDLLPVVSGRASEISHEFATNPTVRKISFTGSTEVGA
                     KIFAGSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKFRNSGQTCVCTNRVYV
                     QSGIYDVFCRKLSEKAAALKLGDGLDEGVNQGPLIEEKAVEKVEQHIADALSKGAVCL
                     TGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFETEAEVIEAANNTEYG
                     LAAYLFTSDTARQWRVGEVLEYGMVGINTGLISNEAAPFGGVKRSGLGREGSKYGADE
                     YLELKYLCIDAG"
     gene            5790..6575
                     /locus_tag="ACSIGJ_001627"
     CDS             5790..6575
                     /locus_tag="ACSIGJ_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HHQ1330453.1"
                     /translation="MMENGKTFPRWRFALKSAGWHLLISLLVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALAYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEKIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIIP"
     gene            6943..8499
                     /locus_tag="ACSIGJ_001628"
     CDS             6943..8499
                     /locus_tag="ACSIGJ_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HHQ1330454.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMVVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESV
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQYAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDGTTALGALELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLEAIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQEEDTDFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            8516..9262
                     /gene="surE"
                     /locus_tag="ACSIGJ_001629"
     CDS             8516..9262
                     /gene="surE"
                     /locus_tag="ACSIGJ_001629"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HHQ1330455.1"
                     /translation="MNVLISNDDGYLAEGIAILARVASEFANVRVVAPERDRSGVSNS
                     LTLDRPLQLKQAQNGFCYVNGTPTDCIHVGQFALPDFKPDVVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGMPAVAFSLNDASGRYWATAEKALWTLLAHFFKKPPSAPVLWNVN
                     IPAVAPEDVRGIKITRLGRRHHEQNIVPSRNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHTD"
     gene            9364..10377
                     /locus_tag="ACSIGJ_001630"
     CDS             9364..10377
                     /locus_tag="ACSIGJ_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HHQ1330456.1"
                     /translation="MLKQTTLLAACTAVAALLGGCATQQPAPVIAGNSGMQTVSSAPV
                     YNPYGATPYNAAPAANDAPYVPPVQTAPVYSPPAYVPPSAPAVSGTYVPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKTAAVE
                     SRPAVPAAAQTPVKPAAQPPVQSAPQPAAPAAENKAVPAPAPAPQSPAASPSGTRSVG
                     GIVWQRPTQGKVVADFGGGNKGVDIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQ
                     HNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSY
                     IAF"
     gene            complement(10775..11257)
                     /locus_tag="ACSIGJ_001631"
     CDS             complement(10775..11257)
                     /locus_tag="ACSIGJ_001631"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HHQ1330457.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRREISLQASGDVSCGC
                     "
     gene            complement(11410..11571)
                     /locus_tag="ACSIGJ_001632"
     CDS             complement(11410..11571)
                     /locus_tag="ACSIGJ_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330458.1"
                     /translation="MSEQPEKHHASPIEDERKKPVYRMGRAVAGFMLAVWAGVLALVF
                     FLVFRFWLS"
     gene            complement(11645..12106)
                     /gene="recX"
                     /locus_tag="ACSIGJ_001633"
     CDS             complement(11645..12106)
                     /gene="recX"
                     /locus_tag="ACSIGJ_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HHQ1330459.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEKTSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAANLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDSC"
     gene            complement(12179..12889)
                     /locus_tag="ACSIGJ_001634"
     CDS             complement(12179..12889)
                     /locus_tag="ACSIGJ_001634"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HHQ1330460.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLVIITNKNEILAAELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDAANMLMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDTTRPDRIIG
                     ALPEIYENLRPQKNKDEE"
BASE COUNT         2966 a         3265 c         3716 g         3119 t
ORIGIN      
        1 gcgacaaaca gttcaatgag tttattttgt ttataccggc ttagacggct ttttctcata
       61 gggataattc taacttaatt tgaatttccc tagttatcta ggacagcgcc ttattataaa
      121 aataccgtct gaagtggcgt tcagacggca tttgtttcaa gcggggctaa cagcagcgtc
      181 cgccgtttgc gccgcagcgg cgtttgcggc aatagtcttg aaattgcagc ttgttcatca
      241 cgggggcgtt gggattatgt ttacgttgtt gtgcaacgta gttttcataa tcgggcacgc
      301 ctgccatcaa gtttgccgtc agcttgatgg ttttccacca agacgcgagc ttatgcttca
      361 ctttgttcct ccggttgttt gccgtcgcgg tacaccgccg ggatttcttt ggcggtcggc
      421 cagccgactt tgcgtgcttt gagggcggta cgcaaaccgt acgcggcaac agtcacgaca
      481 accgacaaga agaggatggt cagaccggca ttaatcttgt cgttgaagat gatttgcgcc
      541 atttcgccga tgtctttagc gggcgcaagg acttcgtttt tagccaatgc gtcgctgtat
      601 ttgcctgcgt gggcaaggaa gctggcgcgc gggtcgttgt ggaacagttt ttgcagaccg
      661 gcgtagcagg ttacaagcag tacgccgacg gcaggaacga gtgccaccca gacataacgg
      721 tcgcgtttca tcttaatcag cacaacggca cacataatca aggctacgcc tgccagcatt
      781 tggttggcga tgccgaataa aggccagagc gagttgatgc cgcccagcgg gtcggttacg
      841 ccggtgtaga ggaagtagcc ccacaatgcc acggcgaaga aggtcgcaat caggttggcg
      901 gggatggagt cggtgttgcc gaaaggtttg tagaagatgc tgcccaagtc ttgaatcata
      961 aaacgtgcga cgcgcgtacc ggcatcgacg gcggtcagga tgaacaaggc ttcaaacaac
     1021 agggcgaagt gataccagaa tgccatcatc gcctcgcccg gaatcagacg gctcataatg
     1081 tgcgccatac cgactgcgag ggtaggcgca ccgcctgtgc gggacaggat ggtgttttcg
     1141 cccacttcct tagcggtgtg caacagggtt gcggcatcga cagggaagtt cagcttggtg
     1201 gtaattactt cggcggcggt gttggcatcc gtaccgatca gtgcggcggg gctgttcatc
     1261 gcgaaataca cgccgggatc gagcgatgcg gcggcggcaa gcgccataat cgccacgaaa
     1321 ctttccatca acataccgcc gtaaccgatc atacggacgt gggtttcgtt ttccagcatt
     1381 ttcggcgtgg tgccggaaga aatcagtgcg tggaagcccg aaaccgcacc gcaggcgatg
     1441 gtaatgaaca agaacgggaa caatgcgcct gagaataccg ggcccgaacc gtcgataaag
     1501 tgggtaaccg caggcatttg caaagcggga ttgacgatga cgatacccaa agccaaggcc
     1561 gcaatcgtac cgattttcag gaaggtggag agatagtcgc gcggggtcag caagagccag
     1621 accggcaaaa ccgaagccac gaaaccgtaa atcataatcg cccaagtgag ctggatgccg
     1681 tcaaggtcaa accaatgccc gatggagctt ttcgccacat cttcgccgta aattaccgcc
     1741 aacatcagca aaataaagcc gacgatggaa atctcgccga ttttgcccgg acggatatgg
     1801 cgcgtgtaaa tacccataaa cagcgcaatc ggcatcgttg cggcaatggt gaacgtaccc
     1861 caagggctgt gaaccaatgc ttttacgaca atcaacgcca aaaccgccat aatgatgacc
     1921 ataatcatca aaataccgat ggaggcaatc acgccgggga cagtgccgag ttcctgtttc
     1981 acaatatcgc ccaaagactt accgtcgcgg cgcatagaga cgaacaagac cgtcatatcc
     2041 tgtaccgcgc cggcaaatac cacgccgaag ataatccaca aagtaccggg tagataaccc
     2101 atttgcgccg ccaaaaccgg accgaccaaa gggcccgcgc cggcaatcgc ggcaaagtgg
     2161 tgtccgaaca atacgccttt gtgcgtcgga acgtagtcca agccgtcgtt gtgccgctcg
     2221 gcgggcgtga gacggttcgg atcgagccgc atcacgcggt tggcgatgta gaggctgtaa
     2281 aaacggtagg cgatacagta aacggatatg gcggcggtaa ccatccatac cgcgctgacc
     2341 tgttcgcctc ggctgagggc cagagtggta aaggatgcta agccgaccag taccactatg
     2401 ccccaaatga ggaaggtttt gagtgatttc atcgaataaa tccttatctc acactgtcgg
     2461 aatatgtcgg agcggacagg caacggttgg ctacccgtaa tccgatgccg acacaaaccg
     2521 gagtttgaat gattgacgaa gtatgaaaca gtgcgcttgt cgggacttgt gccttatgcc
     2581 cgcatcaaac agtgcaggaa tgtacgcgca caaacgttaa acgccgaata gaattttaac
     2641 gcaaattagc acactgatag cggtttttac ttggaatttg gaaaaattta cattcctccg
     2701 ggcgggcagg caggttcaga cggcatcgtc aggcaaaagg cggtgtcgga agacgggtaa
     2761 agaaggggcg cgcaatccgg attattgatt tatcgtgttt ccttttcggt tgaacctcag
     2821 ccctttgggg cggtaaaatc agactttatt tgggaggggc gcaacccctt ccgaatcagg
     2881 acggcacata gggcggtgct ttaatgtgtc gtcctgtgtg ttgaaacata ttttcttttt
     2941 attggggagc ttttatgaat atcaggtttt tcgcgctgac cgtatcggtt ttgtctttgg
     3001 cggcctgtgc cgtgccggaa gcgtatgatg gcggcggacg cgggtatatg ccgcctgttc
     3061 aaaaccaagc cggcccggac gattttcgag cgttttcatg cgagaacggt ttgtctgtgc
     3121 gcgtccgcaa tttggacggc ggcaaaatcg cgttgcggct ggacggcagg cgtgccgtcc
     3181 tctcttccga cgttgccgca tccggcgaac gctataccgc cgaacacggt ttgttcggaa
     3241 acggaaccga gtggcaccag aaaggcggcg aagccttttt cggctttacc gatgcctacg
     3301 gcaattcggt cgaaacttcc tgccgcgccc gttaaacggg gcagcctgtt tgaaccgccg
     3361 tccggatttt tcgggcggtt ttttatgccg gttttgtttt ggccgtcggc tggttccggc
     3421 ggttttcggg cgtggcggat tatttttcgt ttcgttctgg gtttatcctg tcggcagcgg
     3481 ctcaatgcca actttaaacc tgctccgatt tcttcagggc tgttatccaa tgataaaatt
     3541 acatcgtctg catcaatggc atcccacgct tccggcttga catggcggct cgggctgatt
     3601 ttcaggcagc cgttgtgcag ccaaatatcc ccgctcatca tgtttttaaa tagggcgcgt
     3661 ctggttttat agcccaagtt cccgcatagg ttggcaaccc aatcctcata gcgttgccga
     3721 aatttttcgg tatcaaaaaa atcttggtct tccgaactgt cataaacgaa agtcctgctg
     3781 tttgccaacg cttgcaagac cgtcgtgcct aaagtttcat tgtcggtatc caatggcagg
     3841 atatgggggg atataggtgg tctattgccg ttaaccccaa acctgaacat gtttgaacaa
     3901 tcagagcttc ttcattagca taaaaaactg cccaataatt gatcctgttt aaaaatcatt
     3961 atttaatctc cgtaattttg actgtaatgt tttgactttt gccatactct accacgcgtt
     4021 gtaactgcaa tctttgcttc ttatatattg taccctatca aagggctgca ttacttttct
     4081 ttctaaagat tactatcgca taaaaagtat ttttcttaaa cgacagggat gcgcccgtgc
     4141 catattcaaa caccgcccgg atgttgcgct gcccgatcgg atgcttcaga cggcatcgga
     4201 agggtttgca gttttggaat atgaagatga taatgtccgc gagattgacg gcatttgaaa
     4261 gcggttatcc cgtataccgg aggatacgaa atgaacgaat attcccaatt aatcaagcat
     4321 cccgatattt ccctttcccc gatttcagac ggcatcgggg tcggcaatcc ggcgacgggc
     4381 gaaattttgg cgtatgtccg caacacgggt tcggacaagc tgaaaaacct gattcaaaag
     4441 gcggcggcag cgcaaaaatt atgggcggcg aaaaccgcct tggaacgcgc cgacatattg
     4501 tggcgttggt attttttagt taaagaaaac aaagaagcac tggcgcgtct catgacgatg
     4561 gagcagggca aaagcctgac cgaggcgcgc ggcgaaatcg attatgcggc ttcgtttgtg
     4621 cgctggtttg ccgaagaggc gcggcggatt gacggcgatg tgctgacgag tgtgaaagcg
     4681 tcgcaaaaac tggttgtgtt gaaacagccc gtcggcgtta ccgctgcgat tacgccgtgg
     4741 aatttcccgt ccgcgatgat agtcaaacct gcatcgctca cgcctttgag tgcgtatgcg
     4801 ttggctgtac tggcctacga agcgggcgtg ccgcaggatt tgcttcccgt cgtcagcggc
     4861 cgcgcttcgg aaatcagcca tgaatttgcc acgaacccga ccgtgcgcaa aatcagcttc
     4921 accggctcga ccgaagtcgg cgcgaaaatt tttgccggca gcgcggcgga cattaaaaaa
     4981 ctcagtttgg aactgggcgg caacgcgccg tttatcgtgt ttgacgatgc cgatttggac
     5041 aaagccgtcg aaggcgcgct ggcgagcaag ttccgcaaca gcggtcagac ctgcgtctgc
     5101 accaaccgcg tttacgtcca atcgggcatt tacgacgtat tttgccgcaa attgagtgaa
     5161 aaggcagccg cgctcaaatt gggcgacggc ttggacgaag gtgtgaacca aggaccgctg
     5221 attgaggaaa aagcggtaga aaaagtcgaa cagcacatcg ccgacgcact ctccaaaggc
     5281 gcggtctgtc tgaccggcgg caaacgcagc gcgttgggcg gaacgttttt tgagccgact
     5341 gtcttaagcg gcgttacggc gcaaatggcg gtagctcgcg aggaaacctt cgggcctttg
     5401 tgtccggtgt tccgttttga aaccgaagcc gaagtcatcg aggctgcgaa caatacggaa
     5461 tacggtttgg cagcttacct tttcacctcc gacaccgccc gtcaatggcg cgtcggcgaa
     5521 gtcttggaat acggcatggt cggcatcaat acgggcttaa tcagtaatga agcggcaccg
     5581 ttcggcgggg tgaaacgcag cggtttggga cgtgaaggca gcaaatacgg tgcggacgaa
     5641 tatctagaat tgaaatattt gtgcattgat gccgggtgac ggatgccgtc tgaatggcag
     5701 ggtttcagac ggtgtcgcat tttaagcagt ctctatctgt tgtacaatgc gccctgtttt
     5761 tacggttatt tttgatttga atgtaagata tgatggaaaa cggaaaaaca ttcccgagat
     5821 ggcgttttgc cttgaaaagt gcgggctggc acctcttaat cagcttattg gttgcagggc
     5881 tggcggcctt gctggttttt aaggtttggt atccttatcc ttatgccgag ctgacgggcg
     5941 gtctgtcgct ttatcagctg gtggtggcgg tcgatatcgt atgcggcccg ctgctgactt
     6001 taattttggc aagcccgaag aaaaagacaa aggcacgcat ggtcgatttt tccatggtcg
     6061 gcatcatcca gctggcggct ttggcgtacg gtttgcacag cgtttcgctg gcgcgtcccg
     6121 tggtggaagc gtttgaacag gaccgcatga ccattgttac ggcggcggaa gtcgtggtcg
     6181 aagatttgca caaagccccc gaagggctgc aaagcctgtc gtggttcggc atccgccgca
     6241 ttgcattgaa agaacctgag gatgcggatg agaagaacaa gacgctggat ttgtccctga
     6301 aaggtatcga gccgagtatg cgtcccgacc ggtggctgcc gtattccgac aaggaagcag
     6361 aaaaaatccg caaacatctg aaaccgctga aagtcttggc ggatgcgaga aaaacgacgg
     6421 ttgcggacat tctgaaacag gcaggtctcg ccgaagggga ggagctgtat tacctgccgt
     6481 ttaccagcag caggcagaaa gagtggatag tcattaccga taaagagggc aacaccaaag
     6541 gctacgcgcc gatagacggc ttcatcatcc cttaaccatt gggattccgc ccgcactcga
     6601 acatccgttc ttcgcggcgg tagaatcaga ctgtatttga gaggggcgca acccctcaat
     6661 cagggcaaca catagggaga cgctttatgt gccgtccgtg tattgaaaca tttcaaaata
     6721 agcccatgcc gtccgaacat cctttcagac ggcatggcgt tttgccattg ccccgataag
     6781 ctgttaaact gtttaaatta tttcgaccga aggtacacgc acatgaaaga ccccgagcag
     6841 agcagtaagc ccgcccgccg ttttttgtgc gtaccgtctg ttcaggcagc ttgagcgttt
     6901 caggctgcct ttttgcgttt gaacttgagg aacacgaaaa tcatggattt cagttggttg
     6961 gcagaaccgc atacctggat aggttttgcc acgcttttgg tgttggaagt cgtattgggg
     7021 atagacaacc ttgtctttgt ggcgattttg gcaaacaagg tccagcccgc acggcgcgac
     7081 cgcgcacgga ttatcgggct ggggctggca gtcgtcatcc gcatcattat gcttgctttt
     7141 atggcgcaca tcatcacgct gaccgagccg ctgttccaaa tcggcggcct cgccgtttcc
     7201 ggcaaggaca tgattatgct cgcgggcggt attttcctgc tttacaaagc caccaccgaa
     7261 ctgcatgaac gcctcgaagg gcacaaccgt tttaccgttg ccgacaacca aaaaaaacac
     7321 gcgccgtttt ggggcgtggt cgcgcaaatc ctgatactgg atgccgtgtt ttccatcgat
     7381 tcggtcatca ccgccgtggc gatggtcgat cacatcgtcg tggcgatggg cgcggtcgtc
     7441 gtcgcaatgg ttgtcatgat ttccgccagc aaactcttga ccgaatttgt ggacaggcat
     7501 cccaccgtcg tgatgctctg ccttggtttt ttgttgatga tcggtttcag cctgattgcg
     7561 gaagccttcc atttccacat tcccaaaggc tacctctacg ccgccatcgg cttctcgatt
     7621 ttaatcgaac tgtttaacca gatttcgcaa cgcaacagcc gcaaaaacga ctacatcggc
     7681 agctcgtggc gcaagcgcac cgccgaaaac gttttgggta tgatgggcat acgcgaaagc
     7741 gtgcttgctg acgcgggcgg cgaatccgtg gatgacgcgc attttgaaga aaacgaaaaa
     7801 tcgatgatac gcagcgtgct gacgcttgcc gaacgcccga ttatgggggt gatgattcca
     7861 cgccgcgaca tcgaacggct ggacatttcc caaagccgcg aagaacagta tgcccaactg
     7921 caaaacacgc cttacagccg cctgctcgtt gtcggaaagg cgggcgtgga cgaaccgttg
     7981 ggctacatca acaaaaaaga cctgctgtcc caactgctgg aaacaggcgg tctcgacatt
     8041 cagacggcat tgcgccagcc gctcgtcctg cccgacggca cgaccgcctt gggcgcgctc
     8101 gaactcttcc gccaaagcag cgcggattac gctttggtgg tggacgagtt cggcgcggtg
     8161 ttgggtatgg tgacgatgaa agacctgttg gaagccatcg caggcgagtt ccccgaagaa
     8221 ttcgagcgcg aagaagaacc cgccgttcag gggaatcccg atgaaagcct gacggtggaa
     8281 ggcgcgttgg aatatgtgga gctggcatcc caactcaacc tgccgcagca ggaggaagat
     8341 accgatttcc ataccgttgc cgggctgatt atggaagaat tgcaaaccat ccccgatgtc
     8401 ggcgattttg ccgattttca cggctggcgg tttgaagtgg tcgaaaaaga agggcagcgc
     8461 atcgagcggg tcaaaatcac caaattgccc gaagaataag catccaggat aaaaaatgaa
     8521 cgttttgatt tccaacgacg acggctatct ggcagaaggc attgccattc tggcgcgggt
     8581 tgcgtcggaa tttgccaatg tcagggtggt cgcgcccgag cgggacagga gcggggtcag
     8641 caattcgctg acgctcgacc gtcctttgca gctgaaacag gcgcaaaacg gcttctgcta
     8701 cgtcaacggc acgccgaccg actgcatcca tgtcggccag tttgccctgc ccgattttaa
     8761 gcccgatgtc gtgttttcag gtatcaaccg tggcgcaaat atgggcgacg atacgcttta
     8821 ttcggggacg gttgcggcgg cgaccgaagc ctatctgatg ggtatgcctg ctgtggcgtt
     8881 ttctttaaac gatgcttccg gacgctattg ggcaaccgca gaaaaggcac tgtggacatt
     8941 gttggcgcat ttttttaaaa aaccgccgtc cgcgcccgtc ttgtggaatg tcaacatccc
     9001 tgctgtcgca ccggaagatg tcaggggaat caagattacc cgtttgggca gacggcatca
     9061 tgagcagaac atcgtcccgt cccgcaatcc gcgcggcgaa cagatttatt ggatagggcc
     9121 ggtcggcgaa gtttccgatc gggaagaggg gacggatttt ggtgaatgcg gcgcaggttt
     9181 tattaccgta acgccgctgc aaatcgattt gaccgcctat ccggacatgg cggagacggc
     9241 ggccttttgg catacggact gaccgttttt caaataccgt cgggttttgc gcttgagaag
     9301 aacgcgcatt tttattatga tataccgcat tcaggctata tatcggaaca tgaaggttat
     9361 cttatgttga aacaaacgac acttttggca gcttgtaccg ccgttgccgc tctgttgggc
     9421 ggttgcgcca cccaacagcc tgctcctgtc attgcaggca attcaggtat gcagaccgta
     9481 tcgtctgcgc cggtttacaa tccttatggc gcaacgccgt acaatgccgc tcctgccgcc
     9541 aacgatgcgc cgtatgtgcc gcccgtgcaa actgcgccgg tttattcgcc tcctgcttat
     9601 gttccgccgt ctgcacctgc cgtttcgggt acatatgttc cttcttacgc acccgtcgac
     9661 atcaacgcgg cgacgcatac tattgtgcgt ggcgacacgg tgtacaacat ttccaaacgc
     9721 taccatatct ctcaagacga tttccgtgcg tggaacggca tgaccgacaa tacgttgagc
     9781 atcggtcaga ttgttaaagt caaaccggca ggatatgccg caccgaaaac cgcagccgta
     9841 gaaagcaggc ccgccgtacc ggctgccgcg caaacccctg tgaaacccgc cgcgcaaccg
     9901 cccgttcagt ccgcgccgca acctgccgcg cccgctgcgg aaaataaagc ggttcccgcc
     9961 cccgcgcccg ccccgcaatc tcctgccgct tcgccttccg gcacgcgttc ggtcggcggc
    10021 attgtttggc agcgtccgac ccaaggtaaa gtggttgccg atttcggcgg cggcaacaag
    10081 ggtgtcgata ttgccggcaa tgccggacaa cccgttttgg cggcggctga cggcaaagtg
    10141 gtttatgccg gttcaggttt gaggggatac ggaaacttgg tcatcatcca gcacaattcc
    10201 tctttcctga ccgcgtacgg gcacaaccaa aaattgctgg tcggcgaagg tcagcaggta
    10261 aaacgcggtc agcaggttgc tttgatgggc aataccgatg cttccagaac gcagcttcat
    10321 ttcgaggtgc gtcaaaacgg caaaccggtt aacccgaaca gctatatcgc gttctgactt
    10381 tccgtttgca atcaggttaa aaatgccgtc tgaaaggtct tcagacggca tttttgtttt
    10441 ggcggctgtg ttttcaaatc attccgaagg gacggggcgt ataaatttgt ttgactgtgt
    10501 ttgggtattt gcttgatttc atccgtttgc aatggaaaag ccgcttattt ccgttcggag
    10561 ggcggtttta tcggataaaa ggcattttgt ccgactgatt agggctgcat caagggaata
    10621 tgaaaacaca accgcttagt ctcacggctg tgttccaaaa tggcgttgaa aaacggatga
    10681 gccagaatca gccgatttta cttcaataaa tcaaaccatt acaccgaatg tcggaacaat
    10741 gcttgtcctg tctttcagac ggcataggcc gccgtcagca gccgcaggac acgtctccgg
    10801 aggcttggag gctgatttct ctgcgttttt tggctttttc gtagcggcag cgttggcggt
    10861 cggtcaggat ttccagcgga gggacgggga cgggtttgcc gtctttgacc gcaaccatcg
    10921 tgaagtagca gctgttggta tggcggattt cgccggtgcg gatgttttgt gcttcgacac
    10981 ggatgccgat ttccatcgag gtgcgtcccg tgtagttgac agcggcgtaa aaggtaacca
    11041 aatcgccgat gtggatgggt tctttaaaca ggactttgtc aaccgaaagg gtaacgcaat
    11101 aattgccgct gtaacggctg gcgcaggaat aggcaacctg gtcgagcagg agcaggagtt
    11161 cgccgccgtg tacgttgccg ctgaaattgg cggtgtccgg catcatcagt tcggacataa
    11221 tgagttcgtg ggaaggcagt tggcgttgtt gtgtcatggt gtacctgttt taagaaaggg
    11281 tgttttgaaa tgggtgcggg aaaattttag ggaatgggga agtggaattc aagctaaaaa
    11341 aagctatgga gggatagtct gccgaaatgg gccgccgatg ccgtctgaag ccttcagacg
    11401 gcattttgtt caggaaagcc aaaaacggaa aaccaagaaa aacacgagtg ccagcacgcc
    11461 cgcccaaacg gcgagcatga atccggcaac cgcccgtccc atacggtaaa ccggtttttt
    11521 gcgttcgtct tcgatggggg aggcgtggtg tttttcgggt tgttcggaca ttttgttctc
    11581 aatcggaaaa gttggaaacg ggcgtaaggt tacgctgccg tgcaaaaaga tttaagaagg
    11641 aaggtcagca gctgtcttcc caattttcgt cccaagcgtg tttcaatgcc gtctgaacgg
    11701 tatcggcatc aaaaccgcga taggcgagga aacgcgcctg tttttgtttt tctttgaggt
    11761 tggcggcggg gtgtttgaat tttttacgca acacggctat ggcggcttgc ttttcgcttg
    11821 agcggtcggg aagcaggttg cggctggttt tttcatctat gccctgttgc gccaaagcct
    11881 gtttcagcct caatgaaccg tgtttgcggc ttttgctgcg gatataggct tcggcgtagc
    11941 gcaaatccga ctgccagttg cgttcggcaa attcgtttaa cacgttttcc aactcctctt
    12001 cgctttcggc gtgcggtgca agtttgcgtt tcagaccgat gcggctgact tcttggcgcg
    12061 agaggatgtc catcgcgcgg gcgcgtaggg atttttgcgg tttcatggcg gcggtttcgg
    12121 cacggaaaga ttcagacggt atgggaaacg gattccgatg ccgtctgaac agattgtttt
    12181 attcttcgtc tttgtttttc tgcggtcgca ggttttcgta aatttcgggc agcgcgccga
    12241 taatccggtc ggggcgggtc gtatcgtctt gcgagagcag cgtcatatcg ccgtaaccga
    12301 aggtaacgcc gacactcagg cagccggcgg ctttggcggc gatgatgtcg ttgcgcgagt
    12361 cgccgaccat aagcatattg gcggcatcga tacccaaaac ttcggcggcg tgccgcagcg
    12421 gcagcgggct gggttttttt tcgggcaggc tgtctccgcc gagtatcagg ctgaagtagt
    12481 cggcgagtcc cagttgtttt agaagctcgg cggcaaggat ttcgtttttg ttggtaatga
    12541 ttaccagcgg aatgcccaag gatttgagca atgccagccc ggcttcggtt tcgggatagg
    12601 ggcgggtgaa gacgctcaaa tggtcgcggt agtatttcat ataggacacg aaaccttttt
    12661 cccacagttc ggaatcggct tcgcggtcgc ggtcgttggt gaggacgcgg tgaaccagtt
    12721 tgccgatgcc gtcgccgacg tagctttcga ccactttggc gggcagcggt ttcataccga
    12781 gttgttccaa catcgcttct gcggcggcgg caaggtcggg gacggaatcg cacagtgtgc
    12841 cgtccaaatc gaaggcgacg gcttggacgt gttcgatggc tgcattcata atccggtttc
    12901 ctgtcgggtt gaaagggttt attttaacat cgtttgcaca gggcttctaa tgtatagatt
    12961 attaacaatc gcaacaggtt tttcgggcgg tgttttcgga ttttatggcg gggtctggca
    13021 agggtttgaa atcccggcga tgttccaatt aaatttttaa aaaata
//
LOCUS       DBJGGI010000058        13059 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.058, whole genome shotgun sequence.
ACCESSION   DBJGGI010000058 DBJGGI010000000
VERSION     DBJGGI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13059
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.058"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(201..686)
                     /gene="purE"
                     /locus_tag="ACSIGJ_001635"
     CDS             complement(201..686)
                     /gene="purE"
                     /locus_tag="ACSIGJ_001635"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HHQ1330461.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFEYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAISMLANENPELAQKLADFRAKQEQTVLNMELEQ
                     I"
     gene            complement(734..1447)
                     /locus_tag="ACSIGJ_001636"
     CDS             complement(734..1447)
                     /locus_tag="ACSIGJ_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HHQ1330462.1"
                     /translation="MKTKLPLFIIWLSVSASCASVLPVPEGSRTEMPTQENASDGIPY
                     PVPTLQDRLDYLEGKIVRLSNEVEMLNGKVKALEHTKIHPSGRTYVQKLDDRKLKEHY
                     LNTEGGSASAHTVETAQNLYNQALKHYQNGRFSAAAALLKGADGGDGGSIAQRSMYLL
                     LQSRARMGNCESVIEIGGRYANRFKDSPTAPEVIFKIGECQYRLQQKDIARATWRSLI
                     QTYPGSPAAKRAAAAVRKR"
     gene            complement(1447..2115)
                     /locus_tag="ACSIGJ_001637"
     CDS             complement(1447..2115)
                     /locus_tag="ACSIGJ_001637"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHQ1330463.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKAGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEIGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAAFDAPPSVKILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(2126..2311)
                     /locus_tag="ACSIGJ_001638"
     CDS             complement(2126..2311)
                     /locus_tag="ACSIGJ_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HHQ1330464.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELAQERLKNRGLLWDVELNPEEQK
                     NFESAKVAKKPYTYYQD"
     gene            2455..4431
                     /gene="mutL"
                     /locus_tag="ACSIGJ_001639"
     CDS             2455..4431
                     /gene="mutL"
                     /locus_tag="ACSIGJ_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HHQ1330465.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAVD
                     VELEGGGIRLIRVGDNGGGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGNSALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLELPPKAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRANLTE
                     SVSNAGEVLHDITGVTPAPMPSENDSENLFDSASNHPTGNKPDTRNAFGSSGKTAPMP
                     YQAARAPQQHSLSLRESRAAMDTYAELYKKTDDIDLELSQFEQARFGNMPSETPAHKT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGNLQSQHLLIPVTFAASHEECAALADHAETLAGFGLELSDMGGNTLAVRAAPVML
                     GKSDVVSLARDVLGELAQVGSSQTIASHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDTLFLRGQ"
     gene            4526..6649
                     /gene="dnaX"
                     /locus_tag="ACSIGJ_001640"
     CDS             4526..6649
                     /gene="dnaX"
                     /locus_tag="ACSIGJ_001640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HHQ1330466.1"
                     /translation="MTYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYDPPALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIVNQDGEALLAKAQEMAACAV
                     GFDNALGELAILLQQLALIQAVPSALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLGLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPRPEADAAQTPVQTASAAAMPSEGKTAGPVSHQENNDVPPWEDAPDKTETAAGTA
                     RTSAKSIQTASEAETPPENQVSKNKAADNETEASLSEVPSENPIQATPNDEAVETETF
                     AHEAPAEPFYGFPDNDCPPEDGVEIPPPDWANVLPADTAGGGTDEEAEAGGIGGNNTP
                     SAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYRSDTGLMVLAMTAEARATAD
                     KKRLDKIRDTLAQAYGLQLTLQTEDWRDEAGRETPAMQDKRVQAEDRQKAQALLEADP
                     AAQKILQAFGAQWQPESLELAANRP"
     misc_feature    5715..5754
                     /gene="dnaX"
                     /locus_tag="ACSIGJ_001640"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            6730..7065
                     /locus_tag="ACSIGJ_001641"
     CDS             6730..7065
                     /locus_tag="ACSIGJ_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HHQ1330467.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQDL
                     PPGVGDFFR"
     gene            complement(7718..8764)
                     /gene="recA"
                     /locus_tag="ACSIGJ_001642"
     CDS             complement(7718..8764)
                     /gene="recA"
                     /locus_tag="ACSIGJ_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HHQ1330468.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEISDEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            8964..9728
                     /gene="aroD"
                     /locus_tag="ACSIGJ_001643"
     CDS             8964..9728
                     /gene="aroD"
                     /locus_tag="ACSIGJ_001643"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HHQ1330469.1"
                     /translation="MHTSLTVKNTVIGSGRTKIAVPLVARDAADLSSVLSQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQAVRDALPDKPLLFTFRRHCEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSSEDVLTLLSATLEAKRLVAKPVITMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            9748..11763
                     /gene="rep"
                     /locus_tag="ACSIGJ_001644"
     CDS             9748..11763
                     /gene="rep"
                     /locus_tag="ACSIGJ_001644"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HHQ1330470.1"
                     /translation="MMKLNPQQLEAVRYLGGPLFVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNVWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFALMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTRYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNAYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGNYRAKAEIDEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVGEL
                     VSWFARKGERDGKNVIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRIDNLKKSGPAD"
     gene            complement(11833..12891)
                     /gene="dinB"
                     /locus_tag="ACSIGJ_001645"
     CDS             complement(11833..12891)
                     /gene="dinB"
                     /locus_tag="ACSIGJ_001645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HHQ1330471.1"
                     /translation="MSLRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATVKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     PDEAYLDVTRNFKNIPYAGEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGAGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLARGTDEHPVKAERERLQISTEITLPEDLPLGQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGRLVPKNQQQDLWA"
BASE COUNT         3162 a         3822 c         3404 g         2671 t
ORIGIN      
        1 ctgtctgcgg cttcgtcgcc ttgtcctgat ttaaatttaa tccactatat atttcaaaaa
       61 ttgaaacccg cacagattgt taaaccccaa aacaggggcg ggcgaaattc ttccggcaat
      121 gggttcatgc aaacaaaatg ccatctgaac cccgaaaaca gggcttcaga cggcatttgt
      181 gtattcgcgg acggcgggtt ttaaatctgt tccaattcca tgtttaaaac ggtttgttcc
      241 tgtttggctc ggaagtctgc cagtttttgc gccagttcgg ggttttcgtt ggcgagcatg
      301 gagatggcga acaatgcggc atttgccgcg cctgcctcgc cgatggcgaa tgtggcgacg
      361 ggtacgcctt tgggcatttg tacaatcgat aaaagcgaat cttcgccgcg caggtatttg
      421 cttgggacgg gtacgcccaa aacggggacg gtggtcttgg cggcaaccat gccgggtaaa
      481 tgcgccgcgc cgcccgcacc cgcgatgatg gctttgatgc cgcgcgcccg tgcggtttcg
      541 gcgtattcaa acatcaaatc cggagtgcgg tgtgcggaaa cgacgcgcgc ctcatattct
      601 acgccgaact cttcaagaaa ctgcgctgcc tgccgcataa cgggccaatc gctgttgctg
      661 cccatgatga tgccgatttg tatcataaat ccttcttggt gcggatgggg taaaaagcgg
      721 aaaaatggaa aaactatcgt ttgcgtacgg ctgcggcggc gcgttttgcc gccgggctgc
      781 cgggataggt ctgtatcagg ctgcgccaag tcgcccttgc aatgtctttt tgctgaagcc
      841 tgtattggca ttcgccgatt ttgaatatga cttcgggcgc ggttgggctg tctttgaaac
      901 ggttggcgta acgccctccg atttcgatga cagattcaca gttccccata cgcgccctgc
      961 tttgcagcaa caggtacata ctgcgttgcg cgatgctgcc gccgtctccg ccgtccgccc
     1021 ccttcaacaa ggcggctgcg gcagaaaacc tgccgttttg atagtgtttg agtgcctgat
     1081 tgtagaggtt ttgtgcggtt tcgacggtat gtgcggatgc gctgccgcct tcggtattga
     1141 ggtaatgctc tttcaatttg cggtcgtcga gtttttggac gtatgtcctg ccggaagggt
     1201 gtattttcgt atgctccaat gctttgactt tcccgtttaa catttccact tcgttcgaca
     1261 gccggacgat tttgccttcc agatagtcca aacggtcttg cagagtggga acgggatagg
     1321 gaatgccgtc tgaagcattt tcctgtgtcg gcatttcggt tcggctgccc tccggaacgg
     1381 gtaaaacgga agcacaggag gcagacacag acagccaaat gataaaaagc ggtaatttgg
     1441 tcttcattat tttttcagaa gcagggtcaa accgtcgccg acgggcaggg tgatggggac
     1501 aatccgcgta tcgttcggca ggttttgatt gaaatctttg agaattttga cactgggcgg
     1561 cgcatcaaaa gccgcttcgc gcatcaccct tccgttcaac aaaatattgt cgatggcgat
     1621 gatgccgcct tgccggacga gtttgaggca acgctcgaaa tattgcggcg tgggcggttt
     1681 gtctgcgtct atcagtgcca aatcgtagct tccggcttca ccctgtgcaa tcaaatcatc
     1741 caatgtcagc aatgcgggtt gcaggtgcag gctgatttta tgcgccacac cggcctcgtt
     1801 ccaaacctga cgcgccgtat cggtaaaggt tacattgatg tcgcaggcgg taatccgccc
     1861 gtgttcgggc agcgccaatg caagcgcggt gctgctgtat ccggtaaata cgccgatttc
     1921 cagatatttt tccgcgcgaa tcagctttgc cagccaaacc aaaaccgccg cctgttcgcg
     1981 cgcaatcgcc attttgccca tacggtgatg cccggccttc tcacgcagcc gcgtcaaaac
     2041 gggatgttcg ggttcgccga tggcattcaa atagttttgc aggtccggtg cgacattgga
     2101 cagatgggtc gtcatttagg cgggttcagt cttggtaata ggtataaggt tttttcgcca
     2161 cttttgccga ttcaaagttt ttttgctctt cgggattgag ttcgacatcc cacaaaagcc
     2221 ctcgattttt caaacgttct tgcgccaatt caggtttttc ttcgatcagg cggttgagga
     2281 attgtgtggc ttcggattgg tagtggtaca tctttgcgct ccaattttac gggatacggc
     2341 gtgattatac tggtattttc caaacgggat aaacggcttt tatcaagaat acgggcagaa
     2401 agataagggg ttttattata gaataaggcg ttttttgcaa cggaagaccg ccttatgcca
     2461 cgcattgccg ccctgcccga ccacctcgtc aaccaaattg ccgccggcga agtggtcgaa
     2521 cgccctgcca acgccctgaa agaaatcgtt gaaaacagca tagacgcggg cgcaacggcg
     2581 gttgatgtgg aactggaagg cggcggcatc cgcctcatcc gcgtcggcga caacggcggc
     2641 ggcatccacc ccgacgacat cgaactcgcg ctccaccgcc acgccaccag caaaatcaaa
     2701 accctgaacg atttggaaca cgttgccagc atgggctttc gcggcgaagg cttggccagt
     2761 atcgcctccg tcagccgcct gaccctgacc agccgccaag aagacagttc gcatgcgacc
     2821 caagtcaaag ccgaagacgg caaactcagc agccccaccg ccgccgccca ccccgtcggc
     2881 accaccatcg aagccgccga actcttcttc aacacccccg cgcggcgcaa gttcctcaaa
     2941 tccgaaaaca ccgaatacgc tcactgcgcc accatgctcg aacgcctcgc gctggcgcat
     3001 ccgcacattg ccttctcact caaacgcgac ggcaaacaag tgttcaaact tcctgcccaa
     3061 agcctgcatg aacgtatcgc cgccattgtc ggcgatgatt ttcagacggc atctttggaa
     3121 attgacagcg gcaatagcgc gctgcggctc tatggcgcga ttgccaagcc gactttcgcc
     3181 aaaggcaaaa ccgataaaca atattgcttc gtcaaccatc gttttgtccg cgacaaagtc
     3241 atgctccatg ccgtcaagca ggcataccgc gacgtattgc acaacgccct cacccctgct
     3301 ttcgtccttt tcctcgagct gccgcccaaa gccgtggatg tcaacgtcca cccgaccaaa
     3361 accgaaatcc gcttccgcga cagccggcaa gtacaccaac tcgtgttcca cacgctcaac
     3421 aaagcccttg ccgacacacg cgccaacctg accgaaagcg tcagcaacgc aggcgaagtg
     3481 ttgcatgaca ttaccggcgt tacgcctgcc ccaatgccgt ctgaaaacga cagcgaaaat
     3541 ctgtttgata gcgcatccaa ccacccgaca ggcaacaaac ccgatacacg caatgccttt
     3601 ggttcatcag gtaaaaccgc gcccatgcct taccaggccg cgcgtgcgcc gcaacaacac
     3661 agcctgtccc tgcgcgaaag ccgcgcggca atggacacct atgccgaact ctacaaaaaa
     3721 accgacgaca tcgaccttga gttgagccaa ttcgaacagg cacgtttcgg caatatgccg
     3781 tctgaaacgc ctgctcacaa aacagatacg ccgctttcag acggcatccc gtcccaatcc
     3841 gaactgccgc cgctcggttt cgccattgcc caattacttg gcatctacat tcttgcccaa
     3901 gccgaagaca gcctgttgct catcgatatg cacgccgctg ccgaacgcgt caactacgag
     3961 aaaatgaaac gccaacgtca ggaaaacggc aacctgcaaa gccagcacct gcttattccc
     4021 gtaacctttg ccgcgtccca cgaagaatgc gccgccctcg ccgatcatgc cgaaacgctg
     4081 gcaggcttcg gcctggaact gtccgacatg ggcggcaaca ccctcgccgt ccgcgccgcg
     4141 cccgtaatgt tgggcaaatc cgatgtcgtc tccctcgcac gcgacgtatt gggcgaactc
     4201 gcccaagtcg gcagcagcca aaccatcgca tcacacgaaa accgcatcct cgccacaatg
     4261 tcctgccacg gctcaatccg cgccggccgc aggctcaccc tgcccgaaat gaacgcgctg
     4321 ctgcgcgata tggaaaatac gccgcgcagc aaccagtgca accacggcag gccgacgtgg
     4381 gtcaaactga ctttgaaaga attggacaca ctgttcttgc gcggacagta agccgaaaat
     4441 gctagaatac gccgcccgaa gccgccgttc agacggcatt ccgacgcacc gacagaaaca
     4501 tcacgaccga aaccaagaga aaaacatgac ctatcaagtt ctcgcccgaa aatggcggcc
     4561 caaaaccttt tccgacttag tcggtcagga acacgtcgtc aaagccctgc aaaatgcctt
     4621 ggacgaaggc aggctgcacc acgcctacct gctgaccggg acgcgcggcg tgggcaaaac
     4681 caccatcgcc cgcatccttg ccaaaagcct caactgcgaa aatgcgcaac acggcgaacc
     4741 ttgcggcgtg tgccaaagct gcacccaaat cgatgccgga cgctatgtcg acctgctcga
     4801 aatcgacgcc gcctccaaca caggcatcga caacatccgc gaagtcttgg aaaacgccca
     4861 atacgcgccg accgccggca aatacaaagt ctatatcatc gacgaagtgc atatgctttc
     4921 caaaagcgcg ttcaacgcca tgctcaaaac gctggaagag ccgcccgaac acgtcaaatt
     4981 catcctcgcc accaccgatc cgcacaaagt ccccgttacc gtattaagcc gctgcctgca
     5041 attcgtctta cgcaatatga ccgcgcaaca ggttgccgac caccttgccc acgtcctcga
     5101 cagcgaaaaa atcgcctacg acccccccgc cctgcaactt ttgggacgtg ccgccgccgg
     5161 atcgatgcgc gatgccttaa gcctgctcga ccaagccatc gccctaggtt cgggcaaagt
     5221 tgccgaaaac gatgtccgcc aaatgatcgg cgcggttgac aaacaatacc tgtacgaact
     5281 gctgacaggc atcgtcaacc aagacggcga agccctgctg gcaaaagcgc aggaaatggc
     5341 ggcgtgtgcc gtcggctttg acaacgcctt gggcgaactg gccatactgc tgcaacaact
     5401 cgccctgata caggcagtcc cctccgcctt ggcgcacgac gaccccgatt ccgatatttt
     5461 gcaccgcctc gcccaaacca taagcggcga acaaatccag ctttactacc aaatcgccgt
     5521 ccacggcaaa cgcgacctgg gcctcgcccc cgacgaatac gccggcttta tgatgaccct
     5581 gctgcgtatg ctggcgttcg cccccttggc ggcggcttcg tgtgatgcaa acgccgtgat
     5641 tgaaaatacc gaacttcaat ccccgtcggc acaaaccgcc gaaaaggaaa ccgccgcaaa
     5701 aaagccacaa ccgcgccctg aagcggatgc cgcccaaacg cccgttcaga cggcatccgc
     5761 agcagcaatg ccgtctgaag gcaaaactgc cggccccgtt tcccatcaag aaaacaacga
     5821 tgttccgcct tgggaagacg cgccggacaa aaccgaaacc gcagccggca cggcgcgaac
     5881 gtcggcaaaa agcattcaga cggcatccga agccgaaacg ccgcccgaaa accaagtttc
     5941 caagaacaag gcagccgaca acgaaaccga ggcttccttg tccgaagtgc cgtctgaaaa
     6001 ccccattcag gcaacaccga atgatgaagc cgttgaaacg gaaacatttg cacacgaagc
     6061 ccctgcagaa cctttctacg gttttccgga taatgactgc cccccggaag acggcgtgga
     6121 aatcccgccg cccgattggg caaatgtcct ccctgccgat accgcaggcg gagggacgga
     6181 cgaagaagca gaagcgggcg gcatcggcgg aaacaacacg ccgtccgccc cgccgcccga
     6241 attttccacc gaaaactggg cagccatcgt ccggcacttc gcccgcaaac tcggcgcggc
     6301 gcaaatgccg gcgcaacact ccgcgtggac ggaataccgt tccgacaccg gactgatggt
     6361 tttggcaatg accgccgaag cccgcgccac cgccgacaaa aagcggctcg acaaaatccg
     6421 cgacaccctc gcccaagcct acgggctgca actcaccctg caaaccgaag actggcgcga
     6481 cgaagccggc cgggaaaccc ccgcgatgca ggacaagcgt gtccaagccg aagacaggca
     6541 aaaagcacaa gcattgctcg aagccgaccc cgccgcacaa aaaatcctcc aagcattcgg
     6601 cgcgcaatgg cagcccgaat cactggaatt ggcggcaaac cggccataaa cagatataat
     6661 gccgcccgaa cccttcggac ggcattgccg cttccctcat tcattcaaac acaaacagga
     6721 gtattcagta tgttcggaaa agccggatta ggcggcctga tgaaacaggc gcagcaaatg
     6781 caggaaaaca tgaaaaaggc acaagccaaa cttgccgaaa ccgaaatcga aggcgaagca
     6841 ggcaacggct tggtcaaaat cacaatgacc tgcgcgcacg aagtacgcaa aatcgacatc
     6901 agccccgatt tgattcaaga agccgccgat gacaaagaaa tgctcgaaga cctcatcctc
     6961 gccgccctca aatccgcccg gggcaaagcc gaagaaaccg caaacaaaac aatgggcgca
     7021 ttcacccaag atctgccccc cggcgtgggc gacttcttcc gctgaccccg accgtcattc
     7081 ccgcgaacgc gggaatccgg taaccgaaaa accacaggaa tctatcggaa aaaaacagaa
     7141 acccccgccg ccgtcattcc cgcgcaggcg ggaatctaga acgtagaatc tgaaaaaccg
     7201 ttttactcga taaatttccg tgccgacagg tctggattcc cgccttcgcg ggaatgacgg
     7261 catatttttt gcatttgata taaagggtcg tttgaatttt gttcagcaag tgcaaagtat
     7321 tgcacataaa agggcgcagg cttcatccgt aaagtcggcg caggatagag gcaaagcggg
     7381 cgtaggtcgg gctgtagcaa ctgtattttt caccccgtcg ggtaaaaata tagtggatta
     7441 acaaaaaccg gtacggcgtt gcctcgcctt agatcaaaga gaacgattct ctaagatgct
     7501 gaagcaccaa gtgaatcggt tccgtactat agtggattaa atttaaacca gtacggcgtt
     7561 gcctcgcctt gccgtactat ttgtactgtc tgcggctctc cgccttgtcc tgatttttgt
     7621 taatccgcta taccaaaact caaatcaagc cgtccggagg cggctcaaaa aaaacggtac
     7681 ttcgcagcag aagtaccgtt tatcgggatt tcaggtttta ttcttcgggt cgttcgccgt
     7741 cggtttcgtc ctgcgtccct tcggtgatgt gcatttctac gccgttgagg gcgcggattt
     7801 ttgcgtcgat ttcgtcggaa atttcggggt tttctttcag ccagacgcgg acgttgtctt
     7861 tgccctgacc gattttcgcg ccgttgtagc tgtaccacgc gccggatttg ttgatgatgt
     7921 cgtttttcac gccgatgtcg atcaattcgc cttcccaact gatgccttct ccgtagagga
     7981 tgtcaaactc tgcctgacgg aacggggggg cgactttgtt tttgatgact ttgacgcggg
     8041 tttcgttgcc caatacttct tcgccttttt tgatggatcc ggtgcggcgg atgtcgaggc
     8101 ggacggaaga atagaatttc agcgcgttgc cgccggtcgt ggtctcgggg ctgccgaaca
     8161 ttacgccgat cttcattcgg atttggttga tgaacacaac cagcgtattg gtttttttga
     8221 tgtgtccggt cagtttgcgc aaagcctgac tcatcaggcg cgcctgcagt ccgacgtggc
     8281 tgtcccccat atcgccttcg atttcggctt tggggacgag tgcggctacg gaatcgacga
     8341 ctaccatatc tatgccgccc gaacggacga gcgtgtcgca gatttccaaa gcctgttcgc
     8401 cggtatcggg ctgggacagg taaagctctt cgacttttac gccgagtttg cgggcgtaaa
     8461 cgggatcgaa ggcgtgttcg gcatcgacaa aggcgcacac gccgccgttt ttctggcatt
     8521 gggcgacggc ttcgaggcag agtgtggttt tgccggagga ttcggggccg aagatttcga
     8581 cgatgcgccc gcgcggcagg ccgccgactc cgagggcgag gtctaatccg agcgatccgg
     8641 tggaaatgac ttcgaggttt tcttcctgct ggctgccgtc cattttcatg atggcgcctt
     8701 tgccgaaact tttttcgatt tgcgccagtg cggcggcaag ggctttgctt ttgtcgtctg
     8761 acatgggggt tactccggaa caaatgcggt atgtgggctg cggcgcaaca cgggctgcgg
     8821 cgcggaatat gaatcggtcg gtcattatcg catgtttgcc tttcgggggc taccatccgc
     8881 ttcagaccga atgccgtctg aaacattgtc cctcctttgc tataatggcg cccttacttt
     8941 tcccgccagc cgcttgaacc gccatgcaca ccagcctgac cgtcaaaaat accgttatcg
     9001 gaagcgggcg caccaaaatc gccgtgccgc ttgtcgcccg agatgccgcc gatttatcct
     9061 ccgtgctgtc ccaaatcaaa aacctgccct tcgatatcgt cgagttccgc gccgactttt
     9121 tggaatgcgc gggcagcatc ggcgaagtat tgcgccacac gcaggccgtc cgcgacgcgc
     9181 tgcccgacaa accgctgctg tttacgttca gacggcattg cgaaggcggc tcgttcccgt
     9241 gttcggacga ttattatttt gaactgctcg acgcgctgat cgaaagccgc ctgcccgaca
     9301 ttatcgatat cgagctgttt tccggcgaaa ccgccgtccg ccgcgccgtg gcaaatgctc
     9361 aaaaaaacgg catcgccgcc ctgctctgca atcatgagtt tcaccgcacg ccgccgcaag
     9421 aagaaatcgt atgccgtctg aaacagatgg aggactgcgg cgcggacatc tgcaaaattg
     9481 cggtgatgcc gcaaagctcg gaagatgtgc tgactttgct ttccgccacg ctcgaagcga
     9541 aacggcttgt cgccaaaccg gttattacga tgtcgatggg gcagacgggt gcggtcagcc
     9601 ggcttgccgg acaggtgttc ggctcaagca tcacgttcgg ttcgggaacg caaaactccg
     9661 cgccggggca aatcggcgta tccgccctcc gtgcggcact cgactgcctc gaaagcggcg
     9721 cagactgatt tcagacggca tcaaaacatg atgaaactca atccccaaca gctcgaagcc
     9781 gtccgctacc tcggcggccc cctgttcgtc cttgccggcg caggcagcgg caaaaccggc
     9841 gtgattacgc aaaaaatcaa gcatttgatt gtcaatgtcg gctatctgcc gcataccgtc
     9901 gccgcgatta cctttaccaa caaagccgct gcggaaatgc aggagcgcgt cgccaaaatg
     9961 ctgcccaaac cgcaaacgcg cgggctgacg atttgcacgt tccattcttt gggcatgaag
    10021 attctgcgcg aagaggcgaa ccatatcggt tacaaaaaaa acttctccat tctcgactct
    10081 accgacagcg ccaaaatcat cggcgaactc ttaggcggca cgggaaaaga agccgtattc
    10141 aaggcgcagc accagatttc cctttggaaa aacgatttaa aaacgcctga agatgtcgtt
    10201 cagacggcct ctaatgtgtg ggaacaacaa accgcgcgcg tgtatgcaag ttatcaggaa
    10261 accctgcaaa gctatcaggc agtagatttt gacgacttaa tccgcctgcc tgccgtgctg
    10321 ttgcagcaaa acagtgaagt gcgcaacaaa tggcagcggc ggctgcgtta tctgttggtt
    10381 gacgaatgcc aagatacgaa tacctgccaa tttgcgctga tgaaactctt gacaggcgcg
    10441 gaagggatgt ttaccgccgt cggcgacgac gaccagtcca tctacgcatg gcgcggcgcg
    10501 aacatggaaa acctgcgcaa aatgcaggaa aactatccgc agatgaaggt catcaaactg
    10561 gagcaaaact accgctccac cgcgcggatt ctcaaaattg ccaacaaagt catcgaaaac
    10621 aaccccaagc tgtttaccaa aaaactttgg tcgcaattgg gcgaaggcga gccggttaaa
    10681 gtcgttgcct gccaaaacga gcagcacgaa gccgattggg tcgtcagcca aatcgtcaaa
    10741 caaaaactca tcggcggcga caaaacccga tacgccgatt ttgccgtgtt ataccgcggc
    10801 aagcatcagg cacggatttt cgaggaagca ttgcgcggcg cgcgcatccc ctaccgtctc
    10861 tccggcggac aaagcttttt cgacaaagcc gaaatcaaag acgtgttgtc ttatgtgcgg
    10921 ctgcttgcca accccaacga cgatcccgcc tttctgcgcg ccgttaccac gccaaaacgc
    10981 ggcatcggcg atgtaacgct gggcaaactc aacgcctacg cgcacgagca cgaatgcagc
    11041 ctgtatgaag ccgcacaaaa cgaagaagcc cttgccacac tgaacaatac caaccgccaa
    11101 cacctgcaag cctttatgga tatgttcggc aactaccgcg ccaaagccga aatcgacgaa
    11161 gcgggcgagt tcatcaacag cctgctcgaa gaaatcgact atgaaaacca tttgatgcaa
    11221 aacgaagaag gcaaagccgg cgaaatcaaa tggcgcaacg tcggcgagtt ggtatcgtgg
    11281 ttcgcacgaa aaggcgaacg ggacggcaaa aacgtcatcg aactcgccca aaccgtcgcc
    11341 ttgatgacgc ttttggaagg aaaagacgaa gaagaaaccg atgccgtctc gctatccacg
    11401 ctgcacgccg ccaaaggttt ggagtatccg tatgttttcc ttgtcggttg cgaagaaggt
    11461 gttttgccgc acaacgacag catcgaagaa ggcaacgtcg aagaagaacg ccgcctgatg
    11521 tacgtcggca tcacccgtgc caaacgccaa ctcacgctga cccactgtgt caaacgcaaa
    11581 aaacaaggca tatggcaatt ccccgaaccc agccgattca tagacgaaat gccgcaggaa
    11641 gatttgaaaa tcctggggcg caaaggcggc gaaccgattg tcagcaaaga agaaggcaga
    11701 cgtaaccttg ccgatataat cggaaggatc gacaacctaa aaaaaagcgg cccggcggat
    11761 taaaccggag ccgcaatgcc gtctgaaaga cgttcagacg gcatattttt tgaacggcgc
    11821 gcgtcaagcg gtttacgccc acaaatcctg ctgctgattt ttcggtacaa gcctgccgac
    11881 accgataccg atgaggcgga acgcgtcttc cgtctgcggc gggacgcgcg ccatcaacat
    11941 ttgcgcagcc tgcagcagag ctgcgcagtc gggcaatacg gaggaataag tcagcgtgcg
    12001 cgtgatgatg cggaaatcgt aggtcttcag cttgagcgtt acgctttggg cttcgacgtt
    12061 tttgcgcgtg atttgccgcc acaggtcttc ggcaagatgg ggcaggtgtc cggcagcctg
    12121 cccgagcggc aggtcttcgg gcagggtgat ttctgtggaa atttggaggc gttcgcgttc
    12181 ggctttgacg gggtgttcgt ccgtaccgcg cgccaaatca tagaggcggt agccgtagcg
    12241 tccgaaatga tttaagagct cgccgcgctc gaaacggcgc aaatcgcccg ccgtccgcat
    12301 acccagcgac tgcatttttt tcagcgttac cttgcccgct ccggggattt tgcccaaagg
    12361 cagggtttcc aaaaatgcca taactttgtg cggcggcaac acaaactgcc cgttcggctt
    12421 gcgccagtcg gaagcaattt tcgccagaaa tttgttcggc gcgatgcctg cggatgcggt
    12481 caaacccgtt tccgcaaaaa tggcggcacg gatttctttg gcaacttcgc cggcgtaagg
    12541 gatatttttg aaattacggg taacgtcaag ataggcttcg tccggcgaca agggttcgat
    12601 taaatcggta taacgcctga atacggcgtg aatctgcgcg gaaacctgac ggtacaaatc
    12661 gaaatgcgga ggcacataca ccgcttgcgg acacagcctt ttcaccgttg ccaccgacat
    12721 cgcggaatgc agcccgaact gccgtgcctc atacgatgcg gcgcaaatca ccgaacgcgc
    12781 gccctcccac gcgacgacca ccggccgccc tttcaaatgc ggctgctcgc gtagttctac
    12841 cgatgcgtag aacgcgtcca tgtcgatgtg gatgattttg cgtaaagaca tcggctctgc
    12901 cgaggataaa agggatattt tactgcgggc atcggacaaa atccaaatat actaacaggt
    12961 taaatacaaa agagttggct ggattaattt tgaaactcga taaaaataat ggtttttatc
    13021 aagcagaaag aaacagtaat ggaaattatg atatcgtaa
//
LOCUS       DBJGGI010000059        12877 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.059, whole genome shotgun sequence.
ACCESSION   DBJGGI010000059 DBJGGI010000000
VERSION     DBJGGI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12877
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.059"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(90..1217)
                     /locus_tag="ACSIGJ_001646"
     CDS             complement(90..1217)
                     /locus_tag="ACSIGJ_001646"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HHQ1330472.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKALKKISPDLVVPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRITLPMLESLIVLNPDD
                     KDELLHQYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDE
                     FIRAAEQVKGKYPDTVFTALGAIDKPRGSGDLEQPAARDIIRFPGFVNNVSEMIKEHH
                     IFVLPSYYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAEK
                     MIYFIENRAAVRLMGNASYAIAKDKFDAEKVDLKFLDILKA"
     gene            complement(1444..1566)
                     /locus_tag="ACSIGJ_001647"
     CDS             complement(1444..1566)
                     /locus_tag="ACSIGJ_001647"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330473.1"
                     /translation="MPSETFRRHFPNTPPVAGLHGTQISLIKPQITAAIKPPFK"
     gene            complement(1571..2796)
                     /locus_tag="ACSIGJ_001648"
                     /pseudo
     CDS             complement(1571..2796)
                     /locus_tag="ACSIGJ_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3189..4436)
                     /gene="fabF"
                     /locus_tag="ACSIGJ_001649"
     CDS             complement(3189..4436)
                     /gene="fabF"
                     /locus_tag="ACSIGJ_001649"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HHQ1330474.1"
                     /translation="MSQRRVVITGLGQVSPVGNTAAEAWDNLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEATGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGDSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHACKTVISST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            complement(4592..4828)
                     /gene="acpP"
                     /locus_tag="ACSIGJ_001650"
     CDS             complement(4592..4828)
                     /gene="acpP"
                     /locus_tag="ACSIGJ_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HHQ1330475.1"
                     /translation="MSNIEQQVKKIIAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            5060..6067
                     /locus_tag="ACSIGJ_001651"
     CDS             5060..6067
                     /locus_tag="ACSIGJ_001651"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HHQ1330476.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNTVYKLGLIPVTDNRTKPI
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFLEIGTVTRNPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDAMIRNIEKSKYQGVLGINIGKNAVTPIQNAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGGELGALLEALKNKQAQLAAAHGKYIPLAVKIAPD
                     LDEAQIEDIARVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGFPVREKSNRVL
                     KKLAEHIDGALPIIGVGGIMEGGDAAEKIRLGTTAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            6264..6737
                     /locus_tag="ACSIGJ_001652"
     CDS             6264..6737
                     /locus_tag="ACSIGJ_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HHQ1330477.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYENKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLTNKSNIFELKPVLEDLASETRGYSPKNWLYILLNDVFHRKEEFENPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSNHSYEENIARLYSHWEHYLNNGGGQG"
     gene            6751..7086
                     /locus_tag="ACSIGJ_001653"
     CDS             6751..7086
                     /locus_tag="ACSIGJ_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330478.1"
                     /translation="MPSEALSGVQTAFCFPTVYKLSLFLDRNNDLIYLKRLEDMIMGF
                     WGSVGKAAKAVGEGLIEAGNQHKALKMEYAEKSSEELHEIVKSDGFFKNSTREKGAAY
                     AVLKERGEV"
     gene            7280..9970
                     /gene="glnE"
                     /locus_tag="ACSIGJ_001654"
     CDS             7280..9970
                     /gene="glnE"
                     /locus_tag="ACSIGJ_001654"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HHQ1330479.1"
                     /translation="MSDHRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKALTDEDFQ
                     AFADWDKIRAEESEEELARQLRELRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYQDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNGITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKSLVRPFVFRKYLDYGAYEAMRNLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKIAEMGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPISPEQRQLLAESMGFDSYAAFSDGL
                     NVHRNKVNQLFNEILSEPEEQAQSNSEWQWAWQEKPDEEERLGRLKEHGFDAEAVTAR
                     LEQICHGHKYRRLSAHAQPRFDTIVPLFVQAAAEQNNPTDTLMRLLDFLENISLRSAY
                     LAFLNEHPQTLAQLAQIMSQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DGIEACGGDTEAQMDTLRHFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTVIAAAL
                     SCAWADMPKKHRDTPQFAVIGYGKLGGKELGYASDLDLVYLYDDPHPEAGDVYSRLAR
                     RLTNRLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFGKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEMLTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNIAADCGLIDKTLAGQSRTAYRLYRRQ
                     QHNTKLRDAAKTEVSDELLSHYGNVRKLWREVFGEEAATA"
     gene            10394..12601
                     /gene="uvrD"
                     /locus_tag="ACSIGJ_001655"
     CDS             10394..12601
                     /gene="uvrD"
                     /locus_tag="ACSIGJ_001655"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HHQ1330480.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQSGQASVHSIMAVTFTNKAAKEMQTRFGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDSGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLGAPDPHTSRMIGCYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRRHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVAAFVRLIEALRNQVGQMHLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPENISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIASRFVEEIPPEVLHYLSVKKPAFDSYGNTRQTTVQRDKIIDDFKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
BASE COUNT         3072 a         3564 c         3464 g         2777 t
ORIGIN      
        1 gattcccgct ttcgcgggaa tgacggggtt tcgcgggaat ggcgggagtt tgtcagaaat
       61 caccgaaact caaaagccga ccaccttgtt tacgccttca aaatatcgag aaatttcaaa
      121 tcgacttttt cggcatcgaa tttatctttg gcaatcgcat aacttgcatt ccccatcagg
      181 cggacggcag ccctgttttc gataaaataa atcatttttt cggccaagat gcggggattc
      241 caaggttcga tcaggaagcc gttgaccttg tcggcaaccg tttccctgca tccggggaca
      301 tccgtcgtaa tcaccgccct gccgacggcc attgcctcct gagtgctccg gggaacgcct
      361 tccctataat aagacggcaa tacgaatata tgatgttctt ttatcatttc ggaaacattg
      421 ttcacaaaac cggggaaacg gataatatcg cgggcggcag gctgttccaa atccccgctc
      481 ccgcgtggtt tgtcgattgc gcccaaagcg gtaaaaaccg tatcggggta tttgccctta
      541 acctgttccg ccgcccgaat aaactcatca atcccttttt ctttcagaaa tctgccgata
      601 aagaggaatt ttacgggttc tttttcatcg ggaatatccg cctcggaata aggatattgc
      661 cgcaaatcca gaccgattcc gcccaaaata tggatgtttt ttattttgat gccgtattga
      721 tgcagcaact cgtctttgtc gtcggggttt aatacaatca ggctttccaa catcggcagg
      781 gtaatgcggt acaaggcgat taaaatcccc tttatgattt ttgtttttaa cggtatgcct
      841 tccggctgcg gggtaaatgc gaatcccaaa ccttccagca tcccgacgat tctgggcacg
      901 cctgccaatt ttgcggcaaa agtgccgaaa atcacgggtt ttgcgaaata agggacaacc
      961 aaatccggcg atattttttt gagtgcttta aatatgagga aggtggattt tatatcggaa
     1021 aacgggttta gcccgctgcg gtttgaccgg taggtaacgg gtgtaacccc catttccctg
     1081 ataatgtcca actcattgtc ggaaaactcc gatacaaagg catacacctg atggtttttg
     1141 ccgattaatt ttttaatgac gggggcgcgg aaaccgtaaa tgctggatgc gactgttgtg
     1201 ataaaaacga ttttcataag gcggacacct tgaatatagg attggaaatg cggtctgcat
     1261 tgatgaaatg ccggatacgg ggcgcggcgg gcaaaccttt gccaagcgcg ccgcatccga
     1321 cgcttcgccc gcccgggcag gggcggcttc agacggcatt ttatcctgaa atccgctgca
     1381 taacgcccag cgatatacag tatcgcgttt tgtgatgatt ttacacccga agggaaaacg
     1441 ggcttatttg aatggcggtt taatcgcggc ggttatttgc ggtttgataa ggctgatttg
     1501 cgtgccgtgc agtccggcaa cgggcggggt gtttggaaaa tgccgtctga aggtttcaga
     1561 cggcattctg tcatgcggcg gttcggggtt cgcggacggg caggacaagc agggctgccg
     1621 tgccggcgag tacggcatcg gcgtaccaca tccagccgta gtcaccgaat tgtgtaatca
     1681 cgatgccgcc gatgtacgag ccgaggaatc cgccgatttg gtgggtgagc atcaccagtc
     1741 cgaacagggt ggcgaggtag cgcgtgccga agagtttgcc ggtaacggcg gcggtcgggg
     1801 cgacggtggc gagccatgtg aatccgagtg cggcggcgaa aatgtaaaag ttgaggtcgg
     1861 ttttgggtga gaagatgtaa atcagcacca tggcgacgcg tgaggcgtag agtccgaaca
     1921 ggatgtgttt tcctttgaag cgtccggtgc atcagcctga gaacacgcaa ccggcgatgt
     1981 ttgccagtcc gatgatggcg atggatgtcg aggcgacggt ggcgggcagt ccgcacaggg
     2041 cgacttccgt gggtaggtgg gttacgagaa aggcgatgtg gaagccgcag gcgaaaaagc
     2101 tcaggtgcag caggatgtag cttggggttt tgaaggcggt tttgactgct tctccgaggc
     2161 tttgtccgtg tgtggcttgg gtgtgttggg tgtgggcggc gttgttgccg ccggcaagcc
     2221 accatgagac gggcagaatc agcagggcga ttgcgcccca aacgtaaaat gtacccgtcc
     2281 agccgacttc gggcaggacg acgagtcctt gagtccttga accagcggtg cgaacaggaa
     2341 ttgtcctgcc gaaccgcctg cgttgaccag tccggatgcc aagccgcgtt tgtgtgtggg
     2401 gacttgggcg gcgacctgtc ccataatgat ggaaaacccg ccggaaccgg tgccgaatgc
     2461 gagcagcagc ccgacggcaa tcatcagccc ccaataagtc gggatgttgg aggcaatcag
     2521 gcaggcgcag acgagcagga gggcaccgcc gcttaatacc ctgaacgcgc cgaaacggtc
     2581 ggcaagcgcg ccggacaatg gttgcaatac gccccacatc agttggaaaa cggcgatgat
     2641 gaggctgaac tgggcaatgt tcaattcggt ggtgttgacg acgggttgga cgaacagtcc
     2701 gagcgtcatc ctcatgccga tggtaatcag caggatgaat gcggcggcgg caatgaccgc
     2761 ccagagtttg ggtgttttcg atgcggtgtg cgtcataata tgtattttga gtcggttaag
     2821 tttgcgaatt ttaacaaacc ggatgattcg ggctaaataa ttcctgattc tttggatata
     2881 gccaacggta tgtctgccga tgccgtctga aagggcttga tgggcgtttg cggttcgggt
     2941 taatatttgt gccgtatttg ttaattccag ttagtatagt ggattaactt taaaccggta
     3001 cggcgttgcc ccgccttagc tcaaagagaa cgattcccta aggtgctgaa gcaccgggcg
     3061 aaccggttcc gtaccatttg taccgtctgc ggcccgccgc cttgtcctga tttttgttaa
     3121 tccactataa aaatgccgtc tgaaacggtt tcagacggca tttcgatgtc ggcggcggct
     3181 ttgcggaatc agcctttgaa gcgtttgaag accagcgtac cgttggtgcc gccgaagccg
     3241 aaggagttgg aaatggcaac gtcgatttcc gcgtcgcgcg cttcgttggc gcagtagtcc
     3301 aaatcgcagc cggcttcaac gtcttgttca aaaatgttga tggtcggcgg gattttgccg
     3361 tcgtgtatcg ccaaaatgct gtacacggcc tccacgccgc ccgccgcgcc gagcaggtgg
     3421 ccggtcatgg atttggtcga gctgatgacg gttttgcagg cgtgttcgcc gaacgcgcgt
     3481 ttgagggctt tggtttcgtt ggcatcgccc aagggggtgg acgtgccgtg cgcgttgacg
     3541 taatccacgt cttcgggatt gatgccggca tctttcagcg cgcgggtaac ggcaagggcg
     3601 gggccttctt cgttcggcgc ggtgatatgg taagcatcgg aactcatgcc gaagccgacg
     3661 atttcggcgt agattttcgc gccgcgtttt ttggcgtgtt ctaattcttc caacaccaat
     3721 atgcccgcgc cttcgccgat gacgaagccg tcgcggcctt tgtcccacgg acgggaagcg
     3781 gtggcggggt cgtcgttgcg ggtggagagg gctttcatcg cagcaaaacc gcccacgccc
     3841 aaggtgctga ttgcgccttc cgcgccgccg gcaaccatta tgtccgcgtc gccgtatttg
     3901 atcagtcggg cggaatcgcc gatggcgtgc gcgccggtgg tacaggcgga aaccatgccg
     3961 tagctcgggc cgcggtagcc tttgaggatg gtaacgtgtc cggaaatcag gttgatcagc
     4021 gaaccgggga taaagaaagg gttgattttg cgcgcgccgc cttcgattac ggctttgccg
     4081 gtggcctcga tgctgggcag tccgccgatg ccggaaccga tgttcacgcc gatgcggtct
     4141 ttgtcgaggt tttccacatc gtccaaaccc gaatcggcaa tcgcctgcaa tgcggcggcg
     4201 atgccgtagt ggatgaacac gtccatccgg cgcgcttctt tcgcgctgat gtattgtccg
     4261 atgtcgaaac cgcgcacttc gccggcgaca cggctgttga tgtcggatgc gtcaaagcgg
     4321 gtaatcgcgc cgatgccgct tttgccggcg agcaggttgt cccaagcctc tgcggcagtg
     4381 ttgccgacag gggaaacctg acctaagcct gtaatgacta ctcttctctg actcatgata
     4441 acctcgctgt tggttgtcgg aatgggggca tatgcggctg tcgtgcagat gccgtctgta
     4501 atttgcggca ggggttcaaa cagtttgcca tataagggaa aagcctctat tgcgcggtgc
     4561 agcagaggct gttgtgtcgg gcgacgaccg gttagccgtt gtgggcattg atgtagtcga
     4621 tagccagttg gacggtggtg attttttcgg catcttcgtc ggggatttcg cagccgaagg
     4681 cttcttccaa agccataacc agctccacgg tatccaaaga atccgcgccc aagtcgtctt
     4741 ggaaggaaga ttcgtttttc acgtcggctt cgtttacgcc cagttgttca gcaataattt
     4801 tcttaacttg ttgttcgatg tttgacatat cagtcgttcc tttatgcctt gcggcaggtt
     4861 gtttaaggga aataaatcgg tggtattgta ccgattttta atagagtttt ctatctaatg
     4921 cctattatat caatatttgc ggatttgtac atttttgggt gcggcgggtt ttgtcgttca
     4981 agtttgacct gtgtgccgta tgtttggcgg gatttcggtt aaaatggcgg catttccatc
     5041 tgaagcagaa agccctgtca tgtatccact tgcccgccgc atcctgtttg cactcgatgc
     5101 cgaaaaagcc caccacttca cgctcgacgc gctcaacacg gtttataaat tgggcctgat
     5161 tcccgtaacc gacaaccgta ccaaacctat aaaactgatg ggtatggatt tgcccaaccc
     5221 tgtcgggctt gccgcaggtc tcgacaaaaa cggtgaatac atcgacgctt tgggcgcgct
     5281 cggtttcggc ttcctcgaaa tcggcacggt tacgcgcaac ccgcagcccg gcaacccgca
     5341 gccgcgcctc ttccgcgttc ccgaacacca aggcatcatc aaccgaatgg gttttaacaa
     5401 ccacggcatc gacgcgatga tacgcaatat tgaaaaaagc aaatatcaag gcgtattggg
     5461 catcaacatc ggcaaaaacg cggttacgcc catccaaaac gccgccgatg attatttaat
     5521 ctgcctcgaa aaagcctacg cgcacgcaag ctacattacc gtcaacattt cctcgcccaa
     5581 caccaaaaac ctccgcgcgc tgcaaggcgg cggcgaattg ggcgcattgc tcgaagccct
     5641 gaaaaacaaa caggcgcagc ttgccgccgc ccacggcaaa tacatcccgc tagccgtcaa
     5701 aatcgccccc gatttggatg aagcacaaat cgaagacatc gcccgcgtcg tcaaatccgt
     5761 cgaaatggac ggcatcatcg ctaccaacac caccatcgac aaatcaagcc tcggcagcca
     5821 tccgctcgca ggcgagcagg gcggtttgag cggttttccc gtgcgcgaaa aaagcaaccg
     5881 ggtgctgaaa aaactggcgg aacacataga cggcgcattg ccgattatcg gcgtaggcgg
     5941 cattatggaa ggcggggacg cggcagagaa aatccgcttg ggcacgaccg ccgtccaagt
     6001 gtacagcgga ttgatataca aaggcccggc gttggtcaaa gaatgtttga aggcattggc
     6061 gcgatgacgc gccccaccca aaatgccgtc tgaacgcacg ttttgccgtt cagacggcat
     6121 tttcatagat tggtatccga ctgttgaaga ggcaatcagc cgtcatttgg aagacttttc
     6181 gtaacaggcg tcaatcgaaa ggggcggaat gacggggcag gcaaaccgat gcgtgaacac
     6241 ggggcaggca accggagtaa aaaatgaacc ttgatttaac cgctcaaaaa gtccgccttt
     6301 cttggaagga tattctgtgg gggtatgaga ataaatactt gggttgggct gatgtggcag
     6361 cttatgcccg aaaaatgacg ctttcagatc atgatgaacg cgtgttcaag ctatctttaa
     6421 ccaacaaatc caatattttt gaattaaagc ctgttctaga agatttggct tcggaaacga
     6481 ggggttattc ccctaaaaat tggctgtaca tcctcctaaa tgacgtattc catagaaagg
     6541 aagaatttga gaatcctctg ggggaagttg aaaaaattta tgcggatttt gattacccgg
     6601 aagaaataga atcatttgtc aggtatatgc cgcccaaaga cggctatatc ccttctaatc
     6661 atagttatga agaaaatatt gcccggttat attctcactg ggaacactat ttgaacaacg
     6721 gcggcgggca gggttaaaac cggcaatccg atgccgtctg aagcgttatc cggcgttcag
     6781 acggcatttt gttttccgac agtttataaa ctgtcgttgt ttcttgacag aaacaacgac
     6841 cttatttatt tgaaacgatt ggaggacatg attatgggtt tttggggtag tgtgggaaaa
     6901 gcagcaaaag cagtaggcga gggattgatt gaagccggca atcagcataa ggcgttgaaa
     6961 atggaatatg cggagaaatc aagtgaggag ctgcatgaaa tcgtaaagag tgatggtttt
     7021 tttaaaaatt ccacacggga gaaaggtgcg gcttatgctg ttttaaaaga gcgtggcgag
     7081 gtttgaacgg gaaacggcgg catttgccgc tgttttttta tcggcaggca tccgtccgaa
     7141 tatcggggca aggtttcaga cggcatcgaa ggttgctatg atatagtggc ttgactttaa
     7201 accggtacgg ctccgccttg ccctgatttg aagttcatcc gctatcacat tcccgccatc
     7261 ctttcaaacg gaatccgaaa tgtccgacca ccgcctcgac accgcccgcc gccattccct
     7321 gttcctcgcc cgccagctcg acaacggcaa actcaagccc gaaattttcc tgcccatgct
     7381 ggacaaggcg ttgacggatg aggatttcca agcctttgcc gactgggaca aaatccgcgc
     7441 ggaagaaagc gaggaagaat tggcgcggca gttgcgcgag ttgcgccgtt atgtggtgtc
     7501 gcagattatc gtgcgcgata taaaccgcat cagcgatttg aacgaagtta cccgcacgat
     7561 tacgctgttt gccgattttg ccgtcaatac cgcgctggat ttcgcctacg cctattatca
     7621 ggacatgtac ggcacgccga tcgggcgtta taccaaatcg ccgcagcatt tgagcgtggt
     7681 ggcgatgggc aaggcgggcg gctatgagct gaacgtgtct tccgacatcg atttgatttt
     7741 cgtctatccc gaatcgggcg acaccgacgg caggcgcgaa cgggggaatc aggagttttt
     7801 taccaaagtc gggcagaaac tgattgcatt gttgaacggc attactgccg acgggcaggt
     7861 gttccgcgtc gatatgcggc tgcggccgga cggtgattcg ggcgcgctgg tgttgagtga
     7921 aaccgcgctg gagcaatatc tgattacgca ggggcgcgaa tgggaacgct atgcgtggtg
     7981 caaaggccgc gtagtcacgc cgtatccgaa cgacatcaaa tcgctggtgc gccccttcgt
     8041 gttccgcaaa tatctggatt acggcgcgta tgaggcgatg cgcaacctgc accgccaaat
     8101 ccgcagcgaa gtcagcaaaa aaggcatggc ggacaacatc aaactcggcg cgggcggcat
     8161 ccgcgaggtt gaatttatcg cccagatttt ccagatgata cgcggcggcc aaatgcgcgc
     8221 gctgcaactg aaaggcacgc aggaaacgct gaagaaaatt gccgagatgg gcattatgcc
     8281 gtctgaaaac gtcgaaaccc tgcttgccgc ctaccgcttc ctgcgcgatg tcgaacaccg
     8341 cctgcaatac tgggacgacc ggcaaaccca aaccctgccg atctcgcccg aacagcggca
     8401 actgctcgcc gaaagcatgg gtttcgacag ttatgccgct ttttcagacg gcctcaatgt
     8461 tcatcggaac aaagtcaatc agttgttcaa cgaaattttg agcgaacctg aagaacaagc
     8521 gcaaagcaat agcgaatggc aatgggcatg gcaggaaaaa cccgacgaag aagaacggct
     8581 aggccgtctg aaagaacacg ggttcgatgc cgaagccgtc accgcaaggc tcgaacaaat
     8641 ctgccacgga cataaatacc gccgcctttc cgcacacgcc cagccgcgct ttgacaccat
     8701 tgtgccgctg ttcgtacagg cggcggcaga gcaaaacaac ccgacagata cgctgatgcg
     8761 gctgttggat tttctcgaaa acatcagcct ccgctccgcc tatctcgcct tcctcaacga
     8821 acatccgcaa accttggcac aactggcgca gattatgagc caaagctcgt gggtggcggc
     8881 gtatctgaac aaatatccga ttttgttgga cgaactcatc agcgcgcagc ttttggatac
     8941 cgcgtttgat tggcaggcac tcgccgccgc cctttcagac ggcatcgaag cctgcggcgg
     9001 cgataccgaa gcgcaaatgg acacgctgcg ccatttccaa cacgcccaag tcttccgcct
     9061 cgccgtccaa gacctcgccg gattgtggac ggtagaatcc ctctctgacc aactctccgc
     9121 cctcgccgac accgtcattg ccgccgccct ttcgtgcgcg tgggcggata tgcccaaaaa
     9181 acaccgcgac accccgcaat tcgccgtcat cggctacggc aagctcggcg gcaaagaact
     9241 cggctacgcc tccgacctcg acttagtcta tctttacgac gatccccatc ccgaagcagg
     9301 cgacgtgtac agccgcctcg cccgccgcct gaccaaccgg ctttccgccg ccaccggcgc
     9361 gggcagcctc tacgaaaccg acctgcgcct gcgccctaat ggcgacgccg gtttcctcgc
     9421 gcacagcatc gccgcctttg gaaaatacca gcgtgaaaac gcatggacat gggaacacca
     9481 gtccctcacc cgcgcccgct tcatctgcgg cacacccgaa attcagacgg cattcgaccg
     9541 catccgcacc gaaatgctga ctgccgaacg cgaccaaacc gccttggcag gcgaaatcat
     9601 cgaaatgcgc gaaaaaatgt tccccaccca tccgcctgcc gacagcaacg tcaaatacgc
     9661 gcgcggcggc gtggtcgacg tcgaatttat cgtccaatat ctgatacttg cccatgcccg
     9721 ccagtatccg caactcttgg acaactacgg caacatcgcc ctattgaaca tcgccgccga
     9781 ctgcggtttg attgacaaaa ccctcgccgg acaaagccgc accgcctacc gcctctaccg
     9841 ccggcagcag cacaacacca aattgcgcga cgcggcaaaa accgaagtaa gcgacgaatt
     9901 gctgtcccat tacggcaatg tcaggaaatt gtggcgggaa gtgttcgggg aggaagcggc
     9961 aaccgcctga acaaaaaatg ccgtctgaag cctgacaatc cgggtttcag acggcatttg
    10021 tttttggctg tgtctgaaca atcggtacga tttcggttgt ttgcaaattg aaaggcggtt
    10081 attcctatgt tcaggcggca aaccgaaata ttttccccaa ccgatagggc gatatcaaag
    10141 cctattcgaa taccgccttt acggggaagg ggctttccga gcttgaccat ggtcaggctc
    10201 ggcaaatcag cccgcatcct gtcgatgcgt ccgtccaaat cggtttggaa cgccgcaatg
    10261 tgatatccgc ctatcaaggt tttgcgtgcg tgtgtcggcg agggctttgc caaaaccgaa
    10321 atcaaaatca agttgtcagg catccttccg tttctattaa aatagcgcat tccatttttc
    10381 agacggcatc cttatgtttc ccgaccaatc cgcccccaat ctgctgcaag gcttgaaccc
    10441 cgaacaactc tccgccgtaa cctggccgcc gcaatccgcc ctcgttttgg caggcgcagg
    10501 cagcggcaaa acgcgcgtgc tgaccacgcg tatcgcatgg cttttgcaaa gcggacaagc
    10561 cagcgtacac agcattatgg cggtaacgtt taccaacaag gccgccaaag agatgcaaac
    10621 ccgattcggc gcaatgattc ccatcaacgt ccgcgctatg tggctcggca cgttccacgg
    10681 tttgtgccac cgctttttgc gcctgcacca ccgcgacgca ggcctgccgt cttcctttca
    10741 aatcctcgac agcggcgacc agctttccct catcaaacgc ctgctcaaaa gcctcaacat
    10801 cgccgaagaa atcatcgcgc cgcgttcgct gcaaggcttt atcaacgcgc aaaaagaatc
    10861 cggtttgcgc gcttccgtct tgggcgcgcc cgatccgcac acaagccgca tgattgggtg
    10921 ctacgccgaa tacgacaaaa tctgccaacg cgaaggcgtg gtcgatttcg ccgaactcat
    10981 gctccgcagc tacgaaatgc tgcaaagtaa cgaaatcctg cgccggcact accaaaaccg
    11041 cttcaaccac attctggtcg acgagtttca agacaccaac aaactgcaat acgcctggct
    11101 caaactcatg gcgggcggta acgcagcggt atttgccgtc ggcgacgacg accaaagcat
    11161 ttaccgcttc cgcggcgcaa acgtcggcaa catgaccgca ctgatggaag aattccacat
    11221 cgacgcgccc gtcaaactcg aacaaaacta ccgctccgtc ggcaacatcc tcgccgccgc
    11281 caacgccgtt atcgaaaaca acgacgaacg tctcggcaaa aacctgcgca ccgacgccga
    11341 agcaggcgac aaaatccgct actactccgc ctttaccgac ctcgaagaag cccggttcat
    11401 cgtggatgaa accaaagccc tcgaacgcga aggctgggat ttggacgaaa tcgccgtcct
    11461 ctaccgcagc aacgcccaat cgcgcgtcat cgaacaaagc ctgttccgca gcggcatccc
    11521 ctacaaaatc tacggcggcc tgcgctttta cgaacgccaa gaaatcaaac acgccctcgc
    11581 ctacctgcgc ctcgccgtca atcccgacga cgacaacgcc ctcttgcgtg tcatcaactt
    11641 cccaccgcgc ggcatcggcg cgcgcaccgt cgaaaacctt cagacggcct caaacgaaca
    11701 aggcatcacc ctctggcagg cagcctgcaa cgccggcgcg aaagccgcca aagtcgccgc
    11761 cttcgtccgc ctgattgaag ccctgcgcaa ccaagtcgga caaatgcacc tgtccgaaat
    11821 catcgtcggc atcctcaaag acagcggtct gaccgagcac taccgaaccc aaaaaggcga
    11881 caaccaagac cgcctcgaca accttgacga actcgtcaac gccgccatcg agttcaaacc
    11941 cgaagacagc aactttgaaa ccctgcccga aaacatttca gacgaccccg ccttccccat
    12001 cctcgccttc ttgagcaacg ccgccctcga atccggcgaa aaccaagcag gggcaggcga
    12061 aaaagccgtc caactgatga ccgtccacgc cgccaaaggc ttggaattca acgccgtctt
    12121 cctcaccggt atggaagagg ggcgtttccc cagcgaaatg agccttgccg agcgcggcgg
    12181 cctcgaagaa gaacgccgcc tcatgtacgt cgccatcacc cgcgcccgca aacgcctcta
    12241 catcaccatg gcacagcaac gcatgctgca cggacaaacc caattcggca tcgcatcccg
    12301 cttcgtcgaa gaaatcccgc cagaagtatt gcactacctg tccgtcaaaa aacccgcctt
    12361 cgacagctac ggcaacacgc gccaaaccac cgtacaacgg gacaaaatca tcgatgactt
    12421 caaacagccg caaacctacg caggcttccg catcggacaa aacgtccgtc acgccaaatt
    12481 cggcaccggc gtgattatcg atgccgcaga caaaggcgaa tccgcccgac tgaccatcaa
    12541 cttcggcaaa cagggcgtga aagagctgga taccaagttt gcgaaattgg aagcgatgta
    12601 aatttgaaat gcaggccgga tattcgtatc cgacaaaaaa acatttgacg cgtttatcgt
    12661 ttccgaaaaa ccgctgttgg aaatgtcgga ttcgagaatc cgacctacgg caaaaaaccg
    12721 tagcaaggac aaggcaaata gtcgtctgaa aacgggaagg gcaattttgc cgcagccgcc
    12781 gccgtcattc ccgcgaacct acattccgtc attcccacga aagtgggaat ccggaacgca
    12841 aaatctaaag aaaccgtttt atccgataag tttccgc
//
LOCUS       DBJGGI010000060        12735 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.060, whole genome shotgun sequence.
ACCESSION   DBJGGI010000060 DBJGGI010000000
VERSION     DBJGGI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12735
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.060"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            113..592
                     /locus_tag="ACSIGJ_001656"
     CDS             113..592
                     /locus_tag="ACSIGJ_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330481.1"
                     /translation="MFVNEKYPYATLFAGLVFLTLPFALAVHDAFACAFGRAGLLVSV
                     SAGGFGRRGGWDGTVWFVFGVFAFLNVVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAVAWIFKLLLGSAALGGLSGEAVSEYAFAVWLVSMLTLPKRLTRAPVQPVVFHRKK"
     gene            687..1310
                     /gene="rsmG"
                     /locus_tag="ACSIGJ_001657"
     CDS             687..1310
                     /gene="rsmG"
                     /locus_tag="ACSIGJ_001657"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HHQ1330482.1"
                     /translation="MERKERLRAGIAAMGPDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPYIEGAQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLRQAAIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTAHLLKDG
                     GYWAAMKGVYPQGEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            1410..2183
                     /locus_tag="ACSIGJ_001658"
     CDS             1410..2183
                     /locus_tag="ACSIGJ_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HHQ1330483.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVQSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELAARVSGK"
     gene            complement(2929..3513)
                     /gene="rnhB"
                     /locus_tag="ACSIGJ_001659"
     CDS             complement(2929..3513)
                     /gene="rnhB"
                     /locus_tag="ACSIGJ_001659"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HHQ1330484.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKEQAVAWHVAASTPEEIASLNILHATMLAMKRAVYGLAARPEKIFIDG
                     NRIPEHLGIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRRPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(3581..5476)
                     /gene="mnmG"
                     /locus_tag="ACSIGJ_001660"
     CDS             complement(3581..5476)
                     /gene="mnmG"
                     /locus_tag="ACSIGJ_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HHQ1330485.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRSQA
                     DRILYKASIREMLENQENLDLFQQAVEDVTLEGERISGVITAMGAEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGLNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAVEREIQRLKTTWYAPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAIPSERLSAEVVEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGRVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(5649..7076)
                     /locus_tag="ACSIGJ_001661"
     CDS             complement(5649..7076)
                     /locus_tag="ACSIGJ_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HHQ1330486.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDCDNHHFRGGPAYYITHGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            7235..8227
                     /gene="pdxA"
                     /locus_tag="ACSIGJ_001662"
     CDS             7235..8227
                     /gene="pdxA"
                     /locus_tag="ACSIGJ_001662"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HHQ1330487.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAHGEGGLEVLHIPAAEAVEAGWLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLHKGIINAARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            complement(8457..11216)
                     /locus_tag="ACSIGJ_001663"
     CDS             complement(8457..11216)
                     /locus_tag="ACSIGJ_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HHQ1330488.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFLGYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNAGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHAACPRCAGTGVIRGIESTALHVLRMVQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     VPAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKIEVREAAGKTAGQKARADKAET
                     RNNGNRRRNERGDRATERANEAEIQSRNVQPAAPVADAAPPETEGQTGKRRRNGSRNE
                     RGQTAPETAAVAETAVQTAENTPPEPYTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPLI
                     ATPVQTASSAVSNGDALIYDAAEKIHRAAADILPEGAAPKAAAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEAATVPAEEMIQV
                     ETRQG"
     gene            11709..12701
                     /locus_tag="ACSIGJ_001664"
     CDS             11709..12701
                     /locus_tag="ACSIGJ_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HHQ1330489.1"
                     /translation="MKTHEISKGSVSLIGVAEHEAGQRLDNYLIKILKGVPKGYIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFEVVYEDDA
                     LLVVNKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSEDGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAEPPPDLAQFAVMLENGTKM"
BASE COUNT         2434 a         3283 c         4021 g         2997 t
ORIGIN      
        1 accggcgttt ttcgttatga tatatataaa cggttgtttg tgcaactttt cgggcgggcg
       61 gcaaggcggt gcgcggtata atgcggcctg cttcggaaga gggggggcgg cgatgtttgt
      121 gaacgagaaa tatccttatg cgaccctgtt tgcggggctg gtgtttttga cgctgccgtt
      181 tgcgttggcg gtgcatgatg cctttgcatg tgcgttcgga cgggcggggc tgctggtgtc
      241 ggtgtcggcc ggcggattcg gccggcgtgg cggttgggac ggcactgttt ggtttgtgtt
      301 cggtgtgttt gcgtttttga atgtggttgt gtcggcgggc ctgacgaaac tggcgtacaa
      361 aaagatgatg cggcggcatt cgcgttacgc gctgtttctg tcgggcgtgg cggcttgcgc
      421 ggcggtggct tggattttca agctgttgtt gggcagtgcc gccttgggcg ggctgagcgg
      481 ggaggcggtg tcggaatatg cgtttgccgt gtggctggtg tctatgctga cgctgccgaa
      541 acgcctgacg cgcgcgccgg tgcagcctgt ggtgttccac aggaaaaaat aggacggaac
      601 ggaaatgccg tctgaaaccc gatacgcggt ttcagacggc atgtttttcc gctaacatta
      661 cgcctgaata tggacaggaa gcagatatgg aacgtaaaga acgcctgcgt gcaggcattg
      721 ccgcgatggg gccggatatt tcggaaacgg cgcaggacag gcttttggcc tatgtggatt
      781 tgttgaaaaa gtggaacaaa acctacaatc tgaccgccct gcgcgacgag gaaaaaatga
      841 ttgtccatca tcttttggac agcctgacgc tgctgcccta tatcgaaggc gcacagacga
      901 tgctggatgt cggttcgggc ggcggccagc ccggcattcc ggcggcggtg tgccgtccgg
      961 atgtgcagat aaccttgttg gatgcgaata cgaagaaaac ggcttttttg cggcaggcgg
     1021 ctatcgagtt ggggttggac aatgtgcgcg tggtgtcggg gcgcgtggag gcggtttcgg
     1081 acgtgcgtgc cgacgtggtt accagccgtg cgtttgcgga actggcggat tttgtgtcgt
     1141 ggacggcgca cctgttgaaa gacggcggct actgggcggc gatgaagggc gtatatccgc
     1201 agggggaaat cggccgcctg ccgcaggatg tgtgcgtcga aaaagtccaa aggctcgacg
     1261 tgccgggctt ggatgcggaa cgccatatcg tcatcctgag caagcgttga gcgcgcttca
     1321 gacggcatga ataccttttt tgtgcggata aaggtaaaat tccgcactgt ttttcttttt
     1381 ttcaacatca gacgggacac gggcgggaca tgagtgcgaa catccttgcc atcgccaatc
     1441 agaagggcgg tgtgggcaaa acgacgacga cggtaaattt ggcggcttcg ctggcatcgc
     1501 gcggcaaacg cgtgctggtg gtcgatttgg atccgcaggg caatgcgacg acgggcagcg
     1561 gcatcgacaa ggcgggtttg cagtccggcg tttatcaggt cttattgggc gatgcggacg
     1621 tgcagtcggc ggcggtacgc agcaaagagg gcggatacgc tgtgctgggt gcgaaccgcg
     1681 cgcttgccgg cgcggaaatc gagctggtgc aggaaatcgc ccgggaagtg cgtttgaaaa
     1741 acgcgctcaa ggcagtggcg gaagattacg actttatcct gatcgactgt ccgccttcgc
     1801 tgacgctgtt gacgcttaac ggcttggtgg cggcgggcgg cgtgattgtg ccgatgttgt
     1861 gcgaatatta cgcgctggaa gggatttccg atttgattgc gaccgtgcgc aaaatccgtc
     1921 aggcggtcaa tcccgatttg gacatcacgg gcatcgtgcg cacgatgtac gacagccgca
     1981 gcaggctggt tgccgaagtc agcgaacagt tgcgcagcca tttcggggat ttgctttttg
     2041 aaaccgtcat cccgcgcaat atccgccttg cggaagcgcc gagccacggt atgccggtga
     2101 tggcttacga cgcgcaggca aagggtgcca aggcgtatct tgccttggcg gacgagctgg
     2161 cggcgagggt gtcggggaaa taggtcaatc caaatcgggc tgcccgtgcc tttatgctgt
     2221 ttggccgggt gcgttaatat ggcggattaa aataaaaata cttaatatcg tcatttatag
     2281 cggattaaca aaaaccggta cggcgttgcc ccgccccggc tcaaagggaa cgattcccta
     2341 aggcgcccaa gcaccaggcg aaccggttcc gtactatccg tactgtctgc ggcccgccgc
     2401 cttgtcctga tttttgttaa tccgctatat cgtcattccc gcaaaaacaa aaaaatcaaa
     2461 aacagaaaac tgaaatatcg tcattcccgc gcaggcggga atctaggtct gtcggtacgg
     2521 aaacttatcg ggaaaaacgg tttttccaac cctgagactc cggattcctg ttttcgcggg
     2581 aatccggttt tttgagtttc agtcattttt gataaattct tgcagctttg agtttctaga
     2641 ttcccgcttt tgcgggaatg gcggtttgga agttacctga aattcaaaaa aaaaacggaa
     2701 accggacgga ttggattccc gcctgcgcgg gaatgacgga ttttaggttt ttttgatttt
     2761 ctgtttttcg cgggaatgac ggttgggaag tttcccgaaa cttaaaaaca actgaaaccc
     2821 aaaaaactaa attaccgcct gcgggggaat gacggttcgg gttctttctc tttgaagttg
     2881 cgatgctgga aatgccgtct gaagttcaga cggcattttt gtgccggttt aaaacaaggc
     2941 ctgctgcgcg agcaggtttc tgacgggggc gaagtcgcgg cggtgttcgg gcagcacgcc
     3001 gtattgtttg agggcttcca gatgctgctt tgtgccgtaa cctttgtgtt tgtcaaaacc
     3061 gtattgggga cggcgttgcg ccagtgcgta catttccgca tcgcgtgcgg tctttgccaa
     3121 aacggatgcg gcggagattt cgatgatttt gctgtcgcct ttgacgactg cttcggcagg
     3181 gatgcctaaa tgttcgggaa tgcggttgcc gtcgatgaat attttttcgg gacgcgcagc
     3241 caaaccgtaa acggcacgtt tcatggcgag catggtggcg tgcaggatgt tgaggcttgc
     3301 gatttcttcg ggcgtggagg cggcgacgtg ccacgcgacc gcctgttctt taatcatttc
     3361 ggcaagcgcg tcgcgttttt tctcgctgag ttttttggag tcggtcagtc cgggcaggtc
     3421 gaatgtttcc ggaaggatga cggcggcggc aaacacgctg ccgaccaaag gtccgcgtcc
     3481 tgcctcgtcc acgccggcgg tcagtatgtg catgatgttt cctgtcggga tggtacgatg
     3541 ccgtctgaaa atggtttcag acggcatcgt accgatgtgt ttatttcgcg tctttaaacc
     3601 cgcgcttcaa atgcaccatc agcagtgcga cggcggcagg cgtaacgccg gaaatgcggc
     3661 ttgcctgccc gacggtttcg ggtttgtgct ggttgagctt ttgttgcact tctgccgaca
     3721 agcctttgac tctgccgtaa tcgatgccgt cgggcagttt taaggtttcg atgtcgcggc
     3781 ggctgtcgat ttcttcgttt tggcggtcga tatagccttg gtatttgact tggatttcga
     3841 cttgttcgac cacttcggcg gagaggcgtt cagacggtat cgcgccttcg agcgtcatca
     3901 gcgcggcgta atcgaggttc gggcggcgta acaaatcgtg caggttggct tcgcggctga
     3961 gtttttgtcc gaacacgcgg atttgttcgt cttcggcgag tttttgcggc gcgtaccacg
     4021 ttgttttcaa acgttggatt tcgcgttcga cggcttcgcg tttttcgttg aacatgcgcc
     4081 attgcgcttc acccaccaag ccgattttgt agccgtcttc ggtcaggcgc atatcggcgt
     4141 tgtcttccct gagttgcagg cggtattcgg cgcggctggt aaacatccgg taaggttcgt
     4201 tcaggccttt ggtaatgagg tcgtccacca atacgccgag gtaggcttgt tcgcggcgca
     4261 gcaggagcgg atcttgtccg cggacgtatt gcacggcgtt cgcgcccgcc agcaatcctt
     4321 gcgcggcggc ttcttcatag cctgtcgtgc cgttgatttg cccggcgaaa aacagtccct
     4381 caatggtttt ggtttcgagg cttgctttga ggttgcgcgg atcgaagtag tcgtattcga
     4441 tggcgtagcc ggggcgcagg atatgggcgt tttcaagacc cttcatactg cgcacgagcg
     4501 cgatttggat gtcgaacggc aggctggtgg agataccgtt ggggtagtat tcgtgcgtgg
     4561 tcagaccttc gggttcgagg aaaatctggt ggctgtcttt gtcggcgaag cggttgattt
     4621 tgtcttcgat agacggacaa taacgcggac ccacgccttc gattttgccg gtaaacatcg
     4681 ggctgcggtc gaagcctgag cggatgatgt cgtgggtttg cgtgttggta tgcgtaatcc
     4741 agcaggacac ttggcgcggg tgcatttcgg cgttgccgcg cacggacatg acgggaacgg
     4801 gcgtgtcgcc gggctgttcg gtgagttggg aaaagtcaat cgtgcgtccg tcaatacgcg
     4861 gcggcgtgcc ggttttcaga cggccttgcg gcagcttcaa ttcgcgcaaa cgtccgccga
     4921 gcgatttggc ggctgggtcg ccggcgcggc cgccttcgta gttttccaaa ccgatgtgga
     4981 ttttgccgga caagaacgtg cctgcggtca gcaccacggc gcgtgcttta aactccgcgc
     5041 ccatcgcggt gatcacgccg ctgatgcgtt cgccctcgag tgttacgtct tcgacggctt
     5101 gttggaaaag gtcgaggttt tcttggtttt ccaacatttc gcggatggag gctttataca
     5161 ggatgcggtc tgcctgcgag cgcgtggctc gtactgccgc gcctttgctg gcgttcaggc
     5221 ggcggaattg gatacctgat ttgtcggttg ccaacgccat tgcgccgccg agcgcgtcga
     5281 gttcgcgtac caaatgccct ttgccgatgc cgccgataga ggggttgcac gacatttgtc
     5341 cgagcgtttc gatattgtgt gtgagcaaaa gcgtctgcgc gcccatacgg gcggcggcaa
     5401 gcgcggcttc cgtgccggcg tgtccgccgc cgacgacgat gacatcgtag gttttggggt
     5461 aaatcatgtg ggtcatgtgt gtattgccta tcggtttttc agacggcatt ccaagcagga
     5521 tgccgtctga aaaacgaaac ggattcaaaa gtaaagggtt gggattgtac gcctgttcgc
     5581 cctgttttta cagtgcgcgg aaagggaaaa gccgcttcgc ggggaagcgg ctccggtaag
     5641 ggtgggattt accaaacatc ggatttgatg cggcgtttca ggcccggatg ttcggaaagt
     5701 ttgaactcgg ggtctttgcc cattttcagc ttggcggtgt aatcgcgcag cagcataaac
     5761 gccaatggcg agagcagcag gatggcgacg aggttgatcc acgccatgat gcccatcgcc
     5821 atatccgcca tatcccagac caaaggcaca ttggcaaccg cgccgaaata gacccacgcc
     5881 aaaaccagca tacggaaaac ggcggtaatc agccaatggc ttttgatgaa ttggacgttg
     5941 gactcggcat aggcatagtt gccgataacg gtggaaaagg caaacataaa caggatgacg
     6001 gcgaggaaac ccgcgcccca ttgccccact tggctgacaa tcgccgcctg cgtcagcgcc
     6061 gcaccgctca aatcgccata aggctgttgg taaatcaaga tgatgaaggc ggtgcaagaa
     6121 caaacgatga tggtatcgac aaacacgccc agcatttgaa tcataccttg cgaaacaggg
     6181 tgtttcactt cggcggcggc ggcggcgttc ggcgcggaac ccatacccgc ctcgttggaa
     6241 tacaggccgc gtttgatgcc catcatcatc gtttgcgaaa tcagaccgcc gagtaagccg
     6301 cctgctgccg cgtcgaattt gaacgcgccc gaaaaaatct gaccgaacac gtccggaatc
     6361 atcggaatat tggtcaaaat gatgaaaagc gcgataaaga ggtacaaaac cgccatcagg
     6421 ggcacgacga tttccgccgc tttggatatg cgcctgatgc cgccgaagat aatcggcgcg
     6481 gttaaaatca ccagggcgac gccgacataa tgaggctccc agccccacgc cgctttgacg
     6541 gtatcggcga tggtattggt ctgaaccgct tcaaacacaa aaccgaaaca gaaaatcagg
     6601 ctcagggcga acaacacgcc cagccatttc tgccccagac cgtgcgtgat gtagtaggca
     6661 gggccgcccc ggaaatggtg gttgtcgcag tcgcggactt taaagagctg cgccagcgaa
     6721 gattcgacaa acgccgaact catgccgatt aaggcggtta cccacatcca aaacaccgcg
     6781 cccggcccgc cgactttgat ggcgattgcc acgcccgcga tattgcccac gcccacgcgg
     6841 ctggcaaggc cggttacaaa tgcctgaaac ggcgtgatgc cgtgagggtc gtccccctgt
     6901 ttgcggccgc cgagcatttc tttgatgctg cgcccgaaca ggcggaattg gacaaagccc
     6961 gtggttacgg tgaagaaaag ccccgtaccc aaaagcatat aaaccaagta tgaccacatc
     7021 ggatcgttga tggcgccgac ccagccgtgc agccattcgg taaagttctc gttcatatcg
     7081 cttccttaaa gttgaaaccc gcacatatgg cggtatgcaa gcagggttta aattttgtaa
     7141 acgcccattc tagcagattg tcaacaaaat cagaaaaatt tacatcgccg cgcgcctgtg
     7201 gcgttagaat cgcgttttgt ttggagcaaa cacgatgaaa cagcctgttt ttgccgttac
     7261 ttccggcgag cctgccggca tcggccccga tatttgtttg gacttggcgt ttgcacgcct
     7321 gccctgccgc tgcgcggtat tgggcgacaa acacctgttg cgcgcgcgcg ccgaagcctt
     7381 gggcaaaagc gtcgtcctgc gcgacttcga tccggaatca ggcggcgcgc acggagaagg
     7441 cgggctggaa gtgctgcaca tccctgccgc cgaagcggtt gaggcagggt ggctcaatcc
     7501 cgccaacgcc gcctatgtgc tgcaactttt ggacaccgcg ctcgcaggca tttcagacgg
     7561 cattttcgac ggcatcgtta ccgcgccgct gcacaaaggc atcatcaacg ccgcgcgcgc
     7621 aagcacaggt tttttcagcg gacacaccga atatctggcg gaaaaaagcg gcacggggca
     7681 ggtcgtgatg atgcttgccg gcaaaggcct gcgcgtcgcc ctcgtaacga cccacctgcc
     7741 gctgaaagac gttgccgccg ccatcacgca accgctgatt gaaagcgtcg cacgcatttt
     7801 gcatcacgac ttaaaacaca aattcggcat caaaaatccc aaaatcctag tcgccggact
     7861 caatccccac gccggcgaag gcggacacct cggacacgaa gaaaccgaca ccattatccc
     7921 cgcgctcgaa aacctgcgcc gcgaagggat aaacctcgcc ggcccgtatc cggcggacac
     7981 attgttccaa ccgtttatgc tcgaaggtgc ggatgccgta ttggcgatgt accacgacca
     8041 agggctgccc gtgttgaaat accacagctt cgggcagggc gtgaacatca cgctggggct
     8101 gccctttatc cgcacctccg tcgatcacgg caccgcgctc gatttggcgg caaccggcag
     8161 ggcggactcc ggcagcctga taactgccgt ggagaccgcc gtcgagatgg cgcgcggcag
     8221 cctttaaaga tgataaaaga cccgtcattc ccgcgcaggc gggaatccgg accttcgggc
     8281 ggcggcaata ttcaaaggtt atccgaaagt ttgaggttct agattcccgt tttcacggga
     8341 atgacgaaag gtggcgggaa tcacgggaat ggcgaagttt cagacggtat tgcaggtatc
     8401 cgaaaccatg caaaaagagg ttctgcggaa cagaacctct ttttgccgcc gtcggttcag
     8461 ccttgccggg tttcgacttg gatcatttct tcggcaggga cggttgcggc ttcagacggt
     8521 tttggctgtt cggaacggcg caaaccgcgt ccggcttgga cttcgggttg tgccgcccat
     8581 gccttcaatg cggcagggtc ggtttcgatc aggacgagtc cgccggtttg tgcggtttcc
     8641 cgtgcctgtt ccgccgcagc cgtaaaggtt gcggtttcag acggcatttc ctgtgctgcg
     8701 gctttcggtg ccgcgccttc gggcaggatg tcggcggcgg cacggtggat tttttccgcc
     8761 gcatcataaa tcagtgcgtc gccgtttgaa acggcggaag atgccgtctg aacgggtgtg
     8821 gcgatgagcg gatcggcaat gctgacggta atcggcgcgt ttgcgtcggt ttcgccgaaa
     8881 acgtgcgcgg cggcggaacg gactttgtcg gcggtgtcgt gaatattcag gtactgctcg
     8941 attttggcgg cagacgggat attgcgtttt ttgccgtttt gacggcggtc gcgctgattg
     9001 ttgcgttcgc ggcgttcttt ggcatcgcgg ctgtcgcgtt cgcggcggtt gcgttcggat
     9061 ttgggcttgc tgcctttgtc ttctgcggta tacggttcgg gcggcgtgtt ttccgctgtc
     9121 tgaacggctg tttcggcaac ggcggcggtt tccggtgcgg tttggccacg ttcgttgcgg
     9181 ctgccgttgc ggcggcgttt tccggtttga ccttcggttt cgggcggtgc ggcatctgca
     9241 acgggtgcgg caggctgtac gttgcggctt tggatttccg cttcgttggc gcgttcggtg
     9301 gcacggtcgc cgcgttcgtt gcggcggcgg ttgccgttgt tgcgcgtttc ggctttgtcg
     9361 gcgcgcgctt tctgtccggc agttttgccc gccgcttcgc ggacttctat tttgctgcct
     9421 tcgcgtttgc tgcggcgcgg gttttggcgg cggttgttgg cgcggctgcc gctgcggttt
     9481 gccgtgctgc gtttttcgga ggtttcggca gcgggtacgg cttgggtttc gctgccgccg
     9541 aaaatgcgtt tgagccatgc tttgaagctg tcccaccagg aggttttttt ctcgggggcg
     9601 gcagtcgggg cggggctggt gtggcgcacg cctttgacgg cgggttcggg gcgggcggct
     9661 ttggcttttt cgccgccgaa aggtttggcg gattcgtctt cttcaggctc ggcaacgcgc
     9721 ttgtagctcg gttcgccgtc ttcttctacg tcgtcggtgc ggatgcggtt gatttcgtag
     9781 tgcggatttt cgaggtggat gttcggaatc aggacgacgt tcacatccaa acgctcttcc
     9841 attgcaaaca gctcggcgcg tttttcgttc agcaggaagg tggcgacatc gacgggcact
     9901 tgggcgcgca cttcgccggt gttgtccttc atcgcttctt cctgaaccat gcgcaaaacg
     9961 tgcagggcgg tggattcgat gccgcggatc acgccggtgc cggcgcagcg cggacaggcg
    10021 gcgtggctgc tttcgcccaa agcgggtttc aggcgttggc ggctcaattc caaaagtccg
    10081 aaacgggaga gtttgcccat ctgtacgcgg gcgcggtctt ttttgagtgc gtcgcgcagg
    10141 acgttttcca catcgcgctg gtgtttgggg ttttccatgt cgatgaagtc gatgacgacc
    10201 aagccgccca agtcgcgcag gcgcatttgt cgggcgactt cttcggcagc ttccatattg
    10261 gttttgaagg cggtgtcttc gatgtccgcg ccgcgtgtgg cgcgtgcgga gttcacgtcg
    10321 atggagacga gggcttcggt gtggtcgatg acgatcgcgc cgccggaggg caggctgacg
    10381 ctgcgcgaga acgcgctttc gatctggtgt tcgatttgga agcgggaaaa cagcggcgtg
    10441 tggtcttcgt agagtttcag acgacctgcg ttgcccggca tgacgtagct catgaactcg
    10501 gcaacttggt cgtaaacttc ttgattgtcc accaaaattt cgccgatgtc gggacggaag
    10561 tagtcgcgga tggcgcggat gagcaacgag ctttccataa agagcaggta ggggtcgtga
    10621 tgcgcttttc ctgcttcttc gattgcctgc cagagttgtt tgaggtagtt caaatcccat
    10681 tccaactctt ccgcgctgcg gccgatgccg gcggtacgcg cgatgatgct catgccgttc
    10741 ggaatgtcga gttgcgccat cgcggctttg agttcttgac gctcttcgcc ttcgatacgg
    10801 cgggatacgc cgccgccgcg cggattgttc ggcatcaata ccagatagcg tccggcgagg
    10861 ctgatgaagg tggtcagcgc cgcgcctttg ttgccgcgct cgtctttttc gacttggacg
    10921 atgacttcca tgccttcttt gagcacgtcc tgaatgcgcg cgcgcccgcc ttcgtagccg
    10981 aggaaatacg aacgcgatac ttctttaaac ggcaaaaagc cgtggcggtc ggttccgtaa
    11041 tcgacgaaac acgcttccag cgacggctcg atgcgggtaa tgatgccttt gtagatattg
    11101 cctttgcgct gttctttgcc cagcgtttcg atgtccaaat ccagcaggtt ttgtccgtcg
    11161 acgatggcaa cgcgcagctc ttcggcctgc gttgcgttga ataacattct tttcatgatc
    11221 acctcgtgga caggcggcat tcgggcgcgc cggtccggtt cggcattccg taaggctggg
    11281 tttccgatgt cttcggataa aaccggtaat cagtttttga gttgaaaatc cgcagggatg
    11341 cacgttccgg ggaaccgtgt gcggaagggt cggataaaga aggctataaa gatcgatgcg
    11401 gcggtctgcc tgccgcgttc cgaacgctgc ggtcggaaaa atgggcgccg gcttcttctt
    11461 gttatcgtga tgcctgtgtt ttcgggcggt ttgcgttcgg ggcttggggc tccgctgccg
    11521 tcttacttcc gcgccgaaac ggcaaaatca attcaaactt gactacgttc tgcgcctgcc
    11581 ggctgggaac aggcgcaggg aaaatgcttt gcggagtgcg tttttaatat aaaattccgt
    11641 tttaaagtaa accgtttcag ggggcgcggc ggacgcgctt tctgctgaaa cggatgttcg
    11701 gattatagat gaaaacgcac gaaataagca aaggttcggt cagcctgata ggggttgccg
    11761 aacatgaggc gggtcaacgc cttgataact atctgataaa aatcctcaag ggcgtgccca
    11821 agggctatat ccaccgcatt atccgcgccg gcgaggtgcg gttgaacaag aaacgctgca
    11881 aacccgacag ccgtattgcg gagggagata cggtgcggat tccgcctgtg cgcgtggcgg
    11941 agaaggaaat gccgtctgaa aggcgtgccg ccgtaccggc gcgtgcgttt gaggtggtgt
    12001 atgaggacga cgcgctactg gtggtcaata agccttccgg tgtggcggta cacggtggca
    12061 gcggcgtgag tttcggcgtt atcgaacagt tgcgccgcgc ccgtccggag gcgaagtatt
    12121 tggagttggt ccaccgtttg gacaaggata cgagcggctt gttgatggtg gcgaagaaac
    12181 gcagcgcgct cgtcaaactg cacgaagcta tccgtaacga ccaccccaaa aaaatctacc
    12241 ttgcgctggg ggtgggcaaa ctgccggacg acaatttcca tgtcaaactg cccctgttca
    12301 aatataccgg cgcacaaggc gaaaagatgg tacgcgtcag cgaggacggg cagtcggcgc
    12361 atacggtgtt ccgtgtgtta agccgtttct cagacggcat tttgcacggt gtcgggctgt
    12421 cgcacctgac tttggtgcgg gcaacgctga aaacgggccg tacgcaccaa atccgcgtgc
    12481 atctgcaatc tcaaggctgt ccgattgcgg gcgacgaacg ctacggcgat tatcaggcga
    12541 accggcgttt gcagaaattg ggtttgaagc gcatgttttt gcacgcgtcc gagctgcacc
    12601 tgaaccatcc gctcacgggt gagccgctgg tgttgaaggc ggagccgccg ccggatttgg
    12661 cgcagtttgc ggtgatgttg gaaaacggga cgaaaatgtg aaccccgatg ccgtctgaag
    12721 gcttcagacg gcatc
//
LOCUS       DBJGGI010000061        11933 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.061, whole genome shotgun sequence.
ACCESSION   DBJGGI010000061 DBJGGI010000000
VERSION     DBJGGI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11933
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.061"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..486
                     /gene="def"
                     /locus_tag="ACSIGJ_001665"
     CDS             <1..486
                     /gene="def"
                     /locus_tag="ACSIGJ_001665"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HHQ1330490.1"
                     /translation="LQYPDERLHTVAKPVEQVDERIRKLVADMFETMYESRGIGLAAT
                     QVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYEEGCLSVPGIYDAVTRAERVK
                     VEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVERLSQLKQGRIKTKLKKRQKHT
                     I"
     gene            574..1500
                     /gene="fmt"
                     /locus_tag="ACSIGJ_001666"
     CDS             574..1500
                     /gene="fmt"
                     /locus_tag="ACSIGJ_001666"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HHQ1330491.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQDVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     ADIIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGTAGEVLSCSADGLVVACGES
                     ALKITELQPAGGRRMNIAAFAAGRSIEAGAKL"
     gene            1579..2838
                     /gene="rsmB"
                     /locus_tag="ACSIGJ_001667"
     CDS             1579..2838
                     /gene="rsmB"
                     /locus_tag="ACSIGJ_001667"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HHQ1330492.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAHIRAAHPQLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLREREKLAASCKKDDVAKYNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLAVEGIAAKALDEYAVTLEEAVPVNRL
                     PGFAEGLVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARRQEALLDALWQALKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            2804..3403
                     /locus_tag="ACSIGJ_001668"
     CDS             2804..3403
                     /locus_tag="ACSIGJ_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HHQ1330493.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIAATRAEARITDG
                     GRLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPTIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            3403..5523
                     /locus_tag="ACSIGJ_001669"
     CDS             3403..5523
                     /locus_tag="ACSIGJ_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HHQ1330494.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVSFSAMLL
                     LVLSAVLARYVILLLKDRRNGVFGSQIAKRLSGMFTLVAVLPGLFLFGISAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALDLAADNAVSNAVPVQIDLIGTASLSGNMGSVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHQFDQPLPDKEHWEQIQQTGSVRSLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPIPENVAQDAVLIEKARAKYAELSYSKKGLQTFFLVTL
                     LIASLLSIFLALVMALYFARRFVEPILSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLDGLTTGVVVFDEKGRLKTFNKAAE
                     QILGMPLAPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVQVEYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIRAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDDQDAQILTRSTDTIIKQVAALKEMVEAFRNYARAPSLKLENQDLNALI
                     GDVLALYEAGPCRFEAELAGEPLMMAADTTAMRQVLHNIFKNAAEAAEEADMPEVRVK
                     SETGQDGRIVLTVCDNGKGFGKEMLHNAFEPYVTDKPAGTGLGLPVVKKIIGEHGGRI
                     SLSNQDAGGACVRIILPKTVETYA"
     gene            5516..6793
                     /locus_tag="ACSIGJ_001670"
     CDS             5516..6793
                     /locus_tag="ACSIGJ_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HHQ1330495.1"
                     /translation="MRSSDILIVDDEVGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVVMSGHASIDTAVEATKIGAIDF
                     LEKPISLQKLLSAVENALKHGAAQIETGSVFDKLGNSAAIQEMNREVEAAAKRTSPVF
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQTGITFIIGKAERCRVRVIASCSYAAGSDGISCEEKLAGLFSESVVRIPPLSMQHE
                     DIPFLIQGIACNVAESQKITPTAFSDDALAVLSRYDWPGNFEQLQNVVTTLLLEADGQ
                     EIGIGAASSALGRGVPADGTGHMACGFDFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            6884..8071
                     /gene="dprA"
                     /locus_tag="ACSIGJ_001671"
     CDS             6884..8071
                     /gene="dprA"
                     /locus_tag="ACSIGJ_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HHQ1330496.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRSFGSAQNALSAPAEQV
                     APAVRHKHALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRALGGKGIPTVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPANKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLTECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            8097..8558
                     /locus_tag="ACSIGJ_001672"
     CDS             8097..8558
                     /locus_tag="ACSIGJ_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HHQ1330497.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPAGSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            8636..10942
                     /gene="topA"
                     /locus_tag="ACSIGJ_001673"
     CDS             8636..10942
                     /gene="topA"
                     /locus_tag="ACSIGJ_001673"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HHQ1330498.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAAAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEESEDGKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRRWIPVMDKFWKPFIKQVE
                     EKEGIERAKFTTQELNETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLAYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRRGVEKVCPQKECGWKEQIEPPAPQE"
     gene            11029..11825
                     /locus_tag="ACSIGJ_001674"
                     /pseudo
     CDS             11029..11825
                     /locus_tag="ACSIGJ_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684347.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         2930 a         3292 c         3394 g         2317 t
ORIGIN      
        1 ttgcaatatc ccgacgagcg tctgcacacg gtggcaaagc ctgtcgaaca agttgacgag
       61 cgcatccgga agctggttgc cgatatgttt gaaacgatgt acgaatcgcg cggcatcggg
      121 ctggcggcga cgcaggtcga tgtgcacgaa cgcgtggtcg tgatggattt gaccgaagac
      181 cgcagcgaac cgcgcgtgtt catcaacccc gtcatcgttg aaaaagacgg cgaaaccact
      241 tacgaagagg gctgcctgtc cgtaccgggc atttacgacg ccgttacccg cgccgaacgc
      301 gtcaaggtcg aggctttgaa cgaaaaaggc gaaaaattca cgctggaggc ggacgggctg
      361 ctggcgattt gcgtgcagca cgagttagat cacttgatgg gcatcgtgtt tgtcgaacgc
      421 ctttcccaac tcaagcaggg gcggattaag accaaactga aaaaacgtca gaaacatacg
      481 atttgattcg cttcgtcatg ccgtctgaac gctgcaaggc tttcagacgg cacagccttg
      541 tccgacaact ttacgcacac gcaggaacac gctatgaaag tcatctttgc cggcacgccc
      601 gattttgccg ccgccgcctt aaaagccgtt gccgccgccg gttttgaaat cccgctggtg
      661 ctgacccagc ccgaccgtcc gaaagggcgc ggtatgcaac tgaccgcccc gcctgtcaaa
      721 caagccgcgc tggaactcgg tttgcgcgtg gcacagcccg aaaaactgcg caacaacgcc
      781 gaagccctgc aaatgctcaa agaggtcgag gcggacgtaa tggtggttgc cgcgtacggc
      841 ttgattctgc cgcaggacgt gttggatacg ccgaaacacg gctgcctcaa tatccatgct
      901 tcgctgctgc cccgttggcg cggcgcggcg ccgattcagc gcgcgattga ggccggcgat
      961 gccgaaaccg gcgtgtgcat tatgcagatg gacatcggtt tggacaccgg cgatgtggtc
     1021 agcgaacacc gctacgccat ccaacctacc gatactgcca acgaagtcca cgacgcgctg
     1081 atggaaatcg gtgcggcggc ggttgttgcc gatttgcaac agcttcaaag caaaggccgt
     1141 ctgaacgcgg tcaaacagcc cgaagaaggc gtaacttacg cccaaaaact aagcaaagaa
     1201 gaagcgcgta tcgattggag cgaaagcgcg gacattatcg aacgcaaaat ccgcgccttc
     1261 aaccccgtgc ctgccgcgtg ggtcgaatat cagggcaagc cgatgaaaat ccggcgtgcc
     1321 gaagtggtgg cgcaacaagg tacggcaggc gaagtgttgt cctgttcggc ggacggtttg
     1381 gtcgttgcct gcggcgaaag cgcgctgaag attaccgaat tgcagcctgc cggcggcagg
     1441 cgtatgaata ttgcggcgtt tgcggcggga cggtctattg aagcaggggc gaagctgtaa
     1501 atcccttcag acggcattcc gatccgcaaa cgggaatgcc gtctgaaacc atcagttgaa
     1561 gaaagcgaat cacataatat gagtatggca cttgcccaaa aacttgccgc cgacagcatt
     1621 gcggcggttg ccgaagggcg caaccttcag gacgtgttgg cgcacatccg cgcggcgcat
     1681 ccgcagctga cggcgcagga aaacggcgcg ttgcaggata tcgcctacgg ctgccagcgt
     1741 tatttgggca gtttgaaaca tatgctcgcg cagatgctga aaaagcccat cgacaatccg
     1801 cagctcgaaa gcctgctttt ggcggcgttg taccaactgc attacacgcg caacgcgccc
     1861 cacgccgtgg tcaacgaagc tgttgaaagc atagccaaaa tcgggcgcgg gcagtaccgt
     1921 tcgtttgcca acgcggtttt gcgccgcttt ttgcgcgaac gtgaaaaatt ggcggcttcc
     1981 tgcaaaaaag acgatgtggc gaaatacaac ctgccgctgt ggtgggttgc ctacctgaaa
     2041 aaccattatc cgaaacactg gcacaacatc gccgccgcgc tgcaatccca tccgccgatg
     2101 acgttgcgcg tcaaccgccg gcacggcaac gccgaaagct atttggaaaa actggctgtg
     2161 gaaggtatcg cggctaaggc gttggacgaa tatgcggtta cgttggaaga agccgtgccg
     2221 gtaaaccgtc tgcccggttt tgccgaagga ctggtgtcgg tacaggactt cggcgcgcag
     2281 caggcggcgt atctccttaa cccgaaagac ggcgaacgga ttttggacgc gtgcgccgcg
     2341 ccgggcggca agacggggca tattttggaa ctggcggact gccgcgttac cgccttggac
     2401 atcgatgcag gccgtctgaa gcgtgtggag gacaatatcg cccgtttggg ctttcagacg
     2461 gcatcggcgg cgtgtgccga tgcgcgggat ttggcggcgt ggtatgatgg aaagcctttc
     2521 gacgcaatcc ttgccgacgt gccgtgcacc gcttccggcg tggcgcggcg caatcccgac
     2581 gtgaaatggc tgcgccgacc gaccgacgcg ctcaaaaccg cccgccggca ggaagccctg
     2641 ctggacgcac tttggcaggc gttgaaacag ggcggcagaa tgctgcttgc cacctgttcc
     2701 gtgttcgtcg aagaaaacga cgggcaactg caaaaattcc tcaaccgcca tgccgatgcc
     2761 gaaccgatcg aatcgcgggt gcttttaccg aacaaacacc aagatggctt ttattacgcg
     2821 cttattcaaa agcattaaac aatggcttgt gctgttgccg atgctctccg ttttgccgga
     2881 cgcggcggcg gagggcattg ccgcgacccg cgccgaagcg aggataaccg acggcgggcg
     2941 gctttccatc agcagccgct tccaaaccga gctgcccgac cagctccaac aggcgttgcg
     3001 ccggggcgta ccgctcaact ttaccttaag ctggcagctt tccgccccga caatcgcttc
     3061 ttatcggttt aaattggggc aactgattgg cgatgacgac aatattgact acaaactgag
     3121 tttccatccg ctgaccaacc gctaccgcgt taccgtcggc gcattttcca ccgattacga
     3181 cactttggat gcggcattgc gcgcgaccgg cgcggttgcc aactggaaag tcctgaacaa
     3241 aggcgcgttg tccggtgcgg aagcagggga aaccaaggcg gaaatccgcc tgacgctgtc
     3301 cacttcaaaa ctgcccaagc ctttccaaat caacgcattg acttctcaaa actggcattt
     3361 ggattcgggt tggaaacctc taaacatcat cgggaacaaa taatgcgccg cttcctaccg
     3421 atcgcagcca tatgcgccgt cgtcctgctg tacggattga cggcggcaac cggcagcacc
     3481 agttcgctgg cggattattt ctggtggata gtctcgttca gcgcaatgct gctgctggtg
     3541 ttgtccgccg ttttggcacg ttatgtcata ttgctgttga aagacaggcg caacggcgtg
     3601 ttcggttcgc agattgccaa acgcctttcc gggatgttca cgctggtcgc cgtactgccc
     3661 ggcttgttcc tgttcggcat ttccgcgcag tttatcaacg gcacgattaa ttcgtggttc
     3721 ggcaacgaca cccacgaagc cctcgaacgc agccttaatt tgagcaagtc cgcactggat
     3781 ttggcggcag acaatgccgt cagcaacgcc gttcccgtac agatagacct catcggcacc
     3841 gcctccctgt cgggcaatat gggcagtgtg ctggaacact acgccggcag cggttttgcc
     3901 cagcttgccc tgtacaatgc cgcaagcggg aaaatcgaaa aaagcatcaa tccgcaccaa
     3961 ttcgaccagc cgcttcccga caaagaacat tgggaacaga ttcagcagac cggttcggtt
     4021 cggagtttgg aaagcatagg cggcgtattg tacgcgcagg gatggttgtc ggcaggtacg
     4081 cacaacgggc gcgattacgc gctgttcttc cgccagccga ttcccgaaaa tgtggcacag
     4141 gatgccgttc tgattgaaaa ggcgcgggcg aaatatgccg aattgagtta cagcaaaaaa
     4201 ggtttgcaga ccttttttct ggtaaccctg ctgattgcct cgctgctgtc gatttttctt
     4261 gcgctggtaa tggcactgta ttttgcccgc cgtttcgtcg aacccattct gtcgcttgcc
     4321 gagggcgcaa aggcggtggc gcagggtgat ttcagccaga cgcgccccgt attgcgcaac
     4381 gacgagttcg gacgtttgac caagctgttc aaccatatga ccgagcagct ttccatcgcc
     4441 aaagaagcag acgaacgcaa ccgccggcgc gaggaagccg cccgccacta cctcgagtgc
     4501 gtgttggatg ggttgactac cggtgtggtg gtgtttgacg aaaaaggccg tttgaaaacc
     4561 ttcaacaagg cggcggaaca gattttgggg atgccgctcg cccccctgtg gggcagcagc
     4621 cggcacggtt ggcacggcgt ttcggcacag cagtccctgc ttgccgaagt gtttgccgcc
     4681 atcggtgcgg cggcaggtac ggacaaaccg gtccaggtgg aatatgccgc gccggacgat
     4741 gccaaaatcc tgctgggcaa ggcgacggta ttgcccgaag acaacggcaa cggcgtggtg
     4801 atggtgattg acgacatcac cgtgctgata cgcgcgcaaa aagaagccgc gtggggtgaa
     4861 gtggcgaagc ggctggcaca cgaaatccgc aatccgctca cgcccatcca gctttccgcc
     4921 gaacggctgg cgtggaaatt gggcgggaag ctggacgatc aggacgcgca aatcctgacg
     4981 cgttcgaccg acaccatcat caaacaggtg gcggcgttaa aagaaatggt cgaggcattc
     5041 cgcaattacg cgcgcgcccc ttcgctcaaa ctggaaaatc aggatttgaa cgccttaatc
     5101 ggcgatgttt tggccctgta cgaagccggc ccgtgccggt ttgaggcgga acttgccggc
     5161 gaaccgctga tgatggcggc ggatacgacc gccatgcggc aggtgctgca caatattttc
     5221 aaaaatgccg ccgaagcggc ggaagaagcc gatatgcccg aagtcagggt aaaatcggaa
     5281 acggggcagg acggacggat tgtcctgacg gtttgcgaca acggcaaggg attcggcaag
     5341 gaaatgctgc acaatgcttt cgagccgtat gtgacggata agccggcggg aacgggactg
     5401 ggtctgcctg tagtgaaaaa aatcattgga gaacacggcg gccgcatcag cctgagcaat
     5461 caggatgcgg gtggggcgtg tgtcagaatc atcttgccaa aaacggtaga aacttatgcg
     5521 tagcagcgat attttaattg tagacgacga agtcggcatc cgcgacctgc tgtcggaaat
     5581 cctgcaggac gaaggctatt cggtcgcgtt ggcggaaaat gccgaagagg cgcgcaagct
     5641 gcgccatcag gcgcgcccgg cgatggtgct gctggatatt tggatgcccg attgcgacgg
     5701 tatcaccctt ttgaaggagt gggcgaaaaa cgggcagctc aatatgccgg tggtggtgat
     5761 gagcgggcat gccagcatcg ataccgccgt agaagcgacc aaaatcggcg cgatcgattt
     5821 tttggaaaaa ccgatttccc tgcaaaagct gctgtctgcg gtcgaaaacg cgttgaaaca
     5881 cggtgcggcg caaatcgaaa cgggatcggt gttcgacaaa ctgggcaaca gtgccgcgat
     5941 tcaagagatg aaccgcgaag tcgaagcggc ggcaaagcgc acgtcccctg tatttttgac
     6001 gggcgaggcg ggttcgccgt ttgaaaccgt ggcgcgctat ttccataaaa acggtacgcc
     6061 gtgggtcagt ccggcaaggg tcgaatatct gatcgatatg ccgatggaac tgttgcagaa
     6121 ggcggagggc ggcgttttgt atgtcggcga catcgcccaa tacagccgca atatccaaac
     6181 cggcattacc tttatcatcg gcaaggcaga acgctgccgt gtcagggtga ttgcttcgtg
     6241 cagctacgcg gcaggttcag acggcatttc atgtgaagaa aaattggccg ggcttttttc
     6301 ggaatcggtc gtccgtattc cgcccctgag tatgcagcat gaagacattc ccttcctgat
     6361 acaggggatt gcctgcaatg tggcggaaag ccaaaaaatc acgcctaccg cattcagtga
     6421 cgatgcgctt gccgtattgt cccgttacga ctggccgggg aatttcgagc agttgcaaaa
     6481 cgtcgtgaca acgctgctgc tggaggcgga cgggcaggaa atcggtatcg gcgcggcttc
     6541 ttcggcactg gggcgcggtg tgcctgccga cggaacgggg catatggcgt gcgggttcga
     6601 ctttaacctg ccgctgcgcg aattaaggga ggaggtggag cggcgttatt tcgagtacca
     6661 catcgcccaa gaaggtcaga atatgagcaa agtggcgcag aaggtcggtt tggaacgcac
     6721 gcacctttac cgcaaactca aacagctcgg catcggcgtt tcgcgccggg cgggggaaaa
     6781 aaccgaagaa taggcccgga cggccggttt cccggctgcg ggcttttgtt ttcagacggc
     6841 atttggagaa aatgccgtct ggaatcgtaa ggggatggat tttatgacag aggacgaacg
     6901 tttcgcgtgg ctgcaattgg cgtttacgcc ctatatcggc gcggaaagtt tcctgctgct
     6961 gatgcgcagt ttcggcagcg cgcaaaatgc cttgtccgca ccggcggaac aggttgcccc
     7021 ggcggtgcgg cataagcacg cgcttgaggc ttggcgcaat gcggaaaaac gcgctttggc
     7081 gcggcaggcg gcagaagcgg cgttggaatg ggaaatgcgg gacggatgcc gcctgatgct
     7141 gcttcaggat gaagatttcc ccgaaatgct gacgcagggg ctgaccgcgc cgccggtttt
     7201 gtttttgcgc ggcaacgtgc ggctgctgca caagccttcc gccgccatcg tcggcagccg
     7261 ccatgccacg ccgcaggcga tgcggattgc caaagatttc ggcagggcgt tgggtgggaa
     7321 aggcattcct accgtgtcgg gtatggcttc gggcatcgac accgccgccc atcagggcgc
     7381 actggaggca gaaggcggca ccatcgccgt gtgggggacg ggcatagacc gcatttatcc
     7441 gccggccaac aaaaaccttg cctatgaaat cgccgaaaaa gggctgattg tcagcgagtt
     7501 ccccatcggc acgcggccgt atgccggcaa ctttccgcgc cgcaaccgcc tgattgccgc
     7561 gctgtcgcag gttacgctgg tggtcgaggc cgccttggaa tccggttcgc tgattaccgc
     7621 cggcctggcg gcagaaatgg ggcgcgaagt gatggcggta cccggttcga tagacaaccc
     7681 ccacagcaaa ggctgccaca aactgattaa agacggagcg aaactgacgg aatgtttgga
     7741 cgacatcctg aacgaatgcc cggggctatt gcaaaatacg ggtgcttcgt catattctat
     7801 aaataaggat acgcccgaca cgggccgccg cgccgttcag acggcatacg ccccgccgcc
     7861 tgccgcaaaa atgccgtctg aaggggcggc aggcggcaca gccgtcggcg gcatactgga
     7921 taaaatgggt ttcgacccca tccatcccga cgtgcttgcc ggacagttgg ctatgcctgc
     7981 cgcagatttg tatgccgcac tgttggaatt ggaattggac ggcagcgttg ccgccatgcc
     8041 cggcggcaga taccagcgta tccgaacttg aacgcacttt atattaagga acacgaatga
     8101 ccgaagtcat cgcctacctc atcgaacatt tccaagattt cgatacctgc ccgccgcccg
     8161 aagacttggg tatgctgctt gaagaagcgg gtttcgatac gatggaaatc ggcaacaccc
     8221 tgatgatgat ggaagtattg ctcaacagct ccgaattttc cgccgaaccc gccggcagcg
     8281 gcgcattgcg cgtgtacagc aaagaagaaa ccgacaacct gccgcaggaa gtgatggggc
     8341 tgatgcagta tctgattgaa gaaaaagccg tcagctgcga acagcgggaa atcatcatcc
     8401 acgcgctcat gcacatcccc ggcgacgaaa ttaccgtaga taccgccaaa gtgctgaccc
     8461 tgctgctttt atgggcaaac aagagcgagc tgcccgtatt ggtcggcgac gagctgatga
     8521 gcgcgctttt actcgacaac aaacccacga tgaactgaag cggcttcaga cggcgccggc
     8581 ccgcccgatg ccgtctgaaa caccggcgtc aaaaccacca tccagagaac gacaaatggc
     8641 gaaaaacctc ttaatcgtcg aatccccgtc caaagccaaa accctgaaaa aatatttggg
     8701 cggcgatttt gaaatcctcg cgtcctacgg acacgtccgc gacctcgtcc ccaaaagcgg
     8761 cgcggtcgat cccgacaacg gctttgcgat gaaataccaa ctcatcagcc gcaacggcaa
     8821 acatgtcgat gcgattgttg ccgctgccaa agaagccgaa aacatctacc tcgcaaccga
     8881 cccggacagg gaaggcgaag ccatttcctg gcatcttttg gaaatcctca aatccaaacg
     8941 cggcctgaaa aacatcaagc cgcagcgtgt cgtgttccac gaaatcacca aaaacgccgt
     9001 gctcgatgcg gttgcccatc ctcgcgaaat cgaaatggat ttggtcgatg cgcaacaagc
     9061 ccgtcgcgct ctggactatc tggtcggttt caacctctcg ccgttgctgt ggaaaaaaat
     9121 ccgacgcggt ttgagcgcgg gccgcgtgca aagccctgct ttgcgcctga tttgcgaacg
     9181 cgaaaacgaa atccgcgcgt ttgaagcgca ggaatattgg acggtacatc tagacagcca
     9241 caaaggccgc agcaagttca ccgccaaact cgcccaatac aacggcgcga aactcgaaca
     9301 attcgacctg ccgaacgaag ccgctcaagc cgatgtgttg aaagaactcg aaggcaaaga
     9361 ggccgtcgtt accgccatcg aaaagaaaaa gcgcagccgc aaccccgccg cgccgttcac
     9421 cacatccacc atgcagcagg atgccgtgcg taaacttggt ttcaccaccg accgcaccat
     9481 gcgtaccgcc cagcagcttt acgaaggtat agacgtaggg cagggcgcca tcggtctgat
     9541 tacctatatg cgtaccgaca gcgtgaattt ggctgacgaa gcgttaaccg aaatccgcca
     9601 ttacattgaa aacaaaatcg gcaaagaata tctgccgagt gccgccaaac aatacaaaac
     9661 caaatccaaa aacgcccaag aagcccatga agcgatccgc ccgacttccg tgtaccgcac
     9721 gcccgaaagc gtcaaaccct tcctgagtgc agaccagttt aaactttatc aaatgatttg
     9781 gcagcgcacc gtcgcctgtc agatgacgcc cgccaaattc gaccaaacca ccgtcgatat
     9841 taccgtcggc aaaggcgtgt tccgagtaac cggacaagtg caaaccttcg caggcttcct
     9901 cagcgtttac gaagaaagca gcgacgatga agagagcgaa gacggcaaaa aactgcccga
     9961 aatgagcgaa ggcgacaaat tgccggtcga caaactctac ggcgaacaac acttcaccac
    10021 tccgccgccg cgctacaacg aagccacact ggttaaagcc cttgaagaat acggcatcgg
    10081 ccgcccctcg acctacgcca gcatcatctc cacgctcaaa gaccgcgaat acgttaccct
    10141 tgagcaaaaa cgcttcatgc ccaccgacac aggcgacatc gtcaataaat tcctgaccga
    10201 acacttcgcc caatacgtcg attaccactt caccgccaaa ctcgaagacc agcttgacga
    10261 aatcgccgac ggcaaacgcc gctggattcc cgtgatggac aaattctgga aaccgttcat
    10321 caaacaagtg gaagaaaaag agggcatcga acgcgccaaa tttaccacgc aggaacttaa
    10381 cgaaacctgc ccgaaatgcg gcgaacacaa actgcaaatc aagttcggca aaatgggccg
    10441 cttcgtcgcc tgtgccggct atcccgaatg cagctacacg cgcaacgtca acgaaaccgc
    10501 cgaagaagcc gccgagcgca tcgccaaagc cgaggctgaa caagccgaac tcgacggacg
    10561 cgaatgcccc aaatgcggcg gtcggttagc gtataaatac agccgtaccg gcagcaaatt
    10621 catcggctgc gccaactacc ccaaatgcaa acacgtcgag ccgctggaaa aaccgaaaga
    10681 caccggcgtc caatgcccgc aatgcaaaaa aggtaacctc gtcgagcgca aatcccgcta
    10741 cggcaaactg ttttacagtt gcagcaccta tcccgactgc aactacgcca cttggaaccc
    10801 gcccgttgcc gaagaatgcc cgaactgcca ttggccggtc ttgaccatca aaaccactaa
    10861 acgccggggt gtggaaaaag tctgcccgca aaaagaatgc ggctggaaag aacaaatcga
    10921 accgcctgca ccgcaagagt gaagcggttg ggattaagat gaaagaaaat gccgtctgaa
    10981 aggggtttca gacggcattt gtaaattaga aggggctgtc ccagatggct ggggaaattt
    11041 aaattaagtc agaattatcc ctatgagaaa agccgtccaa gccggtacaa acaaaagaaa
    11101 cccatcggac tgtttgccgc aggggtaact gcaagaacgg cagcagggtt ggcagcagtt
    11161 aatcaaaaca cggcagcctg ttattttcta tggcgaaata gtgtttggca catcatcggg
    11221 aaaacttcct ttacacgcat ttggacgaaa tctgcgaaat tatggaggca tacgacatat
    11281 cgtttacgat tacttatccg tcaaaacagt ccgcatttgg aaatgtatga cggcgaagca
    11341 ggggcgggtg aaagttattt ccgtaaaggc agacgcggcc gcagtgccgc cggcaaagcc
    11401 gccgtattcg ggcttttgaa gcgcaacggc aaggtttgta cggttactgt cggacactcg
    11461 aaccgctatt ttattgccta ttatccgcgg acgggtgaaa cccggcagca ttgtttatac
    11521 ggattgttcg tagttacgat gtatcagatt gtaagcggat ttggccgttt ccgtatcaat
    11581 cgcggcacac attttgccga acggcaaaac catatcaacg caattgggaa cttttggaat
    11641 cgggcaaacg tcatttgcgc aagtttgacg gcattcccaa agagcatttc gggctgtatt
    11701 tgaaggagtg cggacggcgt tttgacaaca gtggggtaaa agttcgaatt tccattttaa
    11761 aacaattggt aaagcaggat ttatcccggt tggccgggat tttaaaaaat aaatacattc
    11821 tttaacaaaa caaatcatat cccggcaaaa caaatctgat aaaatacttg gcgtttatta
    11881 acaatctttt taataattta atcttagata tttggagttg atatgcatac ggt
//
LOCUS       DBJGGI010000062        11881 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.062, whole genome shotgun sequence.
ACCESSION   DBJGGI010000062 DBJGGI010000000
VERSION     DBJGGI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11881
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.062"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            170..1389
                     /locus_tag="ACSIGJ_001675"
                     /pseudo
     CDS             170..1389
                     /locus_tag="ACSIGJ_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
     gene            complement(1449..2255)
                     /locus_tag="ACSIGJ_001676"
     CDS             complement(1449..2255)
                     /locus_tag="ACSIGJ_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HHQ1330499.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVTAEYQ
                     PTFRSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDTT
                     DGKGEVDIIPLRQGFWKASVEYKADFPDQSLCQKQANYTTLTFQIGHSHH"
     gene            2447..2656
                     /locus_tag="ACSIGJ_001677"
     CDS             2447..2656
                     /locus_tag="ACSIGJ_001677"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HHQ1330500.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVIELLARVLGKNPETTVV
                     VIDEVDTDNWDIGGKSVSERRKEGR"
     gene            2745..3959
                     /locus_tag="ACSIGJ_001678"
     CDS             2745..3959
                     /locus_tag="ACSIGJ_001678"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HHQ1330501.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQNKGLRDITVNDVYIGN
                     GVSELIAMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITSKTKAIVVINPNNPTGAVYSKEILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHCHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYHQ"
     gene            complement(4031..6610)
                     /gene="clpB"
                     /locus_tag="ACSIGJ_001679"
     CDS             complement(4031..6610)
                     /gene="clpB"
                     /locus_tag="ACSIGJ_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HHQ1330502.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADSSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGEILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEIDG
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGADEVAEIVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQNEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIKVEADGDRL
                     KFA"
     gene            6870..7880
                     /gene="trpS"
                     /locus_tag="ACSIGJ_001680"
     CDS             6870..7880
                     /gene="trpS"
                     /locus_tag="ACSIGJ_001680"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HHQ1330503.1"
                     /translation="MSKKRVLTGVTTTGTPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEQEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFQELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAEFTQMLADGLAWGEAK
                     KLSAAKINAELAELRERYNALTSNPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            7961..8506
                     /locus_tag="ACSIGJ_001681"
     CDS             7961..8506
                     /locus_tag="ACSIGJ_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330504.1"
                     /translation="MLKIPFAVLGGCLLLAACGKSENTAEQPQNAAQSAPKPVFKVKY
                     IDNTAIAGLALGQSSEGKTNDGKKQISYPIKGLPEQNAVRLTGKHPNDLEAVVGKCME
                     TDGKDAPSGWAENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQ
                     NGRYVLEIDSEGAFYFRRRHY"
     gene            8823..9167
                     /locus_tag="ACSIGJ_001682"
     CDS             8823..9167
                     /locus_tag="ACSIGJ_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag473 family lipoprotein"
                     /protein_id="HHQ1330505.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAAQAVESDVKDTAASAA
                     ESAASAVEEAKGQVKDAAADAKASAEEAVTEAKDAAAETKEAVSEAAKDTLNKAADAA
                     QEAADKMKDAAK"
     gene            complement(9377..10885)
                     /locus_tag="ACSIGJ_001683"
     CDS             complement(9377..10885)
                     /locus_tag="ACSIGJ_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HHQ1330506.1"
                     /translation="MTTNPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSTSSQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPCGGKMLVIDIGGSSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQAAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMGGIIGGNEMLWYAVLSLRLAALFCRSRQDLGFP
                     KNMQLRTDTESCGFILRIDRKWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(11048..11881)
                     /locus_tag="ACSIGJ_001684"
                     /pseudo
     CDS             complement(11048..11881)
                     /locus_tag="ACSIGJ_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273017.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
BASE COUNT         2741 a         3019 c         3194 g         2927 t
ORIGIN      
        1 gcgctgcttt tttcagacgg atgctgtgcg gcttgaaaaa taaaatcata tatagattaa
       61 taaaatcggg cttatgtcga ctacatgcta ttacgttttg tcgggaaatt ggttatagta
      121 atgagcgaaa tgcacgcccc tgaaaatctt tttgcgaaaa ggaagcaaaa tgtctgatgc
      181 cgttgcaaaa gaaaccctca atccgtttga aatcgcgcga aaacaggtca aaaccgcctg
      241 cgaccggctg aaaaccgatc cggccgttta tgaaatcctg aagagtccga cccgcgtgtt
      301 ggaagtcaaa ctcgacgacg gcacggtcaa aacctttacc ggttaccgtt cgcgtgcgtt
      361 cccatcccgg tgtgaatctg gacgaagtca aggcactgtc gatttggatg accatcaaat
      421 gctgcgttgc aggcattcct tacggcggcg gcaaaggcgg cgttaccttg gatccgcgcg
      481 attattccga ggcggaactg gaacgcatcg cccgagctta tgccgaagcg attgccccgc
      541 tgatcggcga aaaaaatcga tattcccgcc cccgatgtga acaccaacgg caaaattatg
      601 tcgtggatgg tggatgccta tgaaaacgtg gtgaaacatt ccgcgccggg cgtatttacc
      661 ggcaaaccgg ttgagttcgg cggctctttg gcgcgtaccg aggctaccgg ttacggcgtg
      721 aacctcgccg ccgtccaagc tttggaaaaa ctgggcaaag atgtgaaagg cgcaacctac
      781 gccattcaag gtttcggcaa tgtgggctac cacaccggct attacgcgca tcaatccggc
      841 gcgaaagtcg ttaccgtatc tactgttgac gttgctatct acaatgaaaa cggcttggat
      901 atggaagcgc tgtttaaaga gttccaagaa aaaggcttca tcaccaacaa agccggttac
      961 ggcaaagaaa ttaccaacgc cgaacttttg gctttggata tggacgtact cgccccttgc
     1021 gcattggaaa accagctgac ttccgaaaac gccggtaaag tccgcgcgaa aatcgtggtt
     1081 gaaggcgcaa acggcccgac tacgcccgaa gccgacgtta tcctgcgtca aaacggcgta
     1141 ttggtcgtgc ccgatatttt ggcaaactgc ggcggcgtgg tcgtttccta tttcgagtgg
     1201 gtgcaaaacc tgcaaggcta ttattgggag tttgacgaag ttcaggaaaa agaaatcgtc
     1261 gtcctgcgcc gcgcgttccg cgatatttgg aacttggcgc aagagtatga tgtcgatttg
     1321 cgtaccgcgt cttatatgat gagtatccgc cgtgttgaaa aagcgatgaa gctgcgcggc
     1381 tggtattgat gtttgaacaa aggatgccgt ctgaaaccat ttcagacggc atttttgttt
     1441 ggacggaatt aatggtgaga atggccgatt tggaaggtta aagttgtgta gttcgcctgt
     1501 ttttggcaca ggctttgatc ggggaaatcg gctttgtatt cgacactcgc tttccaaaag
     1561 ccttggcgca aggggatgat gtccacttcg cctttgccgt cggtggtgtc ggagaaggct
     1621 tgggcttcgg ttttgtgcgt tttgctgcgg tcgctggtgt cgaagccgtc aaatgtagcg
     1681 gtaacggtgg cattgggcag cggttcgcca cggaacagaa cgcggacttt gaagcgttcg
     1741 cctacgtgaa tgttggcggg attgtccagc gggacgattt ccaagttttg tccgaccggt
     1801 ttggtgatga tggcggtgtc cgcgctttcg tgtcccacgt tgacaatgtt tttaccgaac
     1861 atacgggttt gttcgcaata gcttgcgtca ggcatttctt tgatgccagc ctgtttccag
     1921 cctgctttgt tttttgaccg gaaagtaggc tgatattcgg cggtaacgag gtagctgccg
     1981 tctttgacgg gacggttgct gcggtattgg tagttgtatg tgccgcgttg aatcatgttt
     2041 tccttacctt tttcggtaac cagctgcatc ggtttgctga agatgtgcag gcggtctttg
     2101 gcgatgggtt cgagttcggg gaattcgccg tagcccaagt cggctttaag gtattcgccg
     2161 ccgtgcgtgt gggcggtttc gacccagacg cggtgggcgt gtgcggatgt ggcaaatagt
     2221 gcggaaacgg cgagcagtgt caatgttttc ttcatggtgc agcctttata tcgtgcgggt
     2281 taaaaatttt gttatgctat aacaaaatat gtatcgtgtg aagacggatt tgccgcctga
     2341 aaactgtctt tttgtatcga tttgaatttt tcagaaaatg aagcggacgt ttgggcggag
     2401 gcaacttgtt tgataagata gcaatatttc aaaacggaga aatatcatgc cttatgtcaa
     2461 tattaaagta accggcggca aggaagcacc gactgccgcg caaaaagcgg aactgatcgg
     2521 cggcgtaatc gaattgctgg cacgcgtgct gggtaaaaat cccgaaacaa cggttgtcgt
     2581 gattgacgaa gtggataccg ataactggga tataggcggc aaaagcgtca gcgaacggcg
     2641 caaagagggc aggtaaaaag cctgaaaacc ccgccctgat gctttaaatt tcgcgtgaaa
     2701 aagagtacat tcccacccat tgcccaaaat ttacggaaca cattatggat aaatttccca
     2761 agtctgcaaa gctcgaccac gtctgctacg acatacgcgg gccggttcac aaaaaagccc
     2821 ttcagttgga agaggagggc aacaaaatcc tcaagctcaa tatcggcaac cctgcgccgt
     2881 tcggctttga agcacctgat gaaatcttag tcgatgtcat ccgcaacctg ccgacttcgc
     2941 aaggctattg cgattcgaag gggttgtatt ccgcacgcaa agccattgtt cactactatc
     3001 agaacaaagg tttgcgcgat attacggtaa acgatgtcta tatcggcaac ggcgtgtccg
     3061 agctgattgc gatgtctatg caggcgttgc tcaacgacgg cgacgaaatc ctgattcccg
     3121 cgcccgacta ccctttgtgg acggcggcgg caacgcttgc gggcggtacg gtacgccatt
     3181 atctgtgcga cgaagaaaac ggctggtttc ccaaccttgc cgatatggaa gccaaaatca
     3241 cgtccaaaac caaagccatc gtcgtcatca accccaataa cccgacagga gcggtgtaca
     3301 gcaaggaaat cctgctggaa attgccgaac tggcgcgcaa gcatggtttg attattttcg
     3361 ccgacgagat ttacgacaaa atcctttatg atggcgcggt tcactgccac atcgccgcgc
     3421 tcgcccccga ccttttgacg gtaacgttca acggtttgtc caaagcctac cgtgtagccg
     3481 ggttccgcca aggctggatg gtgctaaacg ggccgaaaca tcacgcaaaa ggttatatcg
     3541 agggtttgga tatgctctcg tccatgcgct tgtgtgccaa tacgccgatg cagcacgcga
     3601 ttcagacggc attgggcggt tatcagagca ttaacgaatt catcttgccc ggcggacggc
     3661 ttttggaaca gcgcaacaga gcgtgggaac tggtcaacca gattcccggc gtgtcctgcg
     3721 tcaaaccgat gggcgcgatg tatatgttcc cgaaaatcga taccgaaatg taccgcatcc
     3781 gcgatgatat gaaattcgtt tacgatttgc tggtgcgcga aaaagtcctg ctggtacagg
     3841 gaacggggtt taattggatc aaacccgacc atttccgcat tgttacgctg ccttacgtcc
     3901 atcagattga agaggcgatg ggcaggttgg caagattcct gcaaacctac catcagtagg
     3961 attctgataa aaaatgccgt ctggaacgga gattcccgtt tcagacggca ttttcaacag
     4021 caggaacaaa tcaggcaaat ttcagtctgt cgccgtcggc ttctaccttg atttcgcttt
     4081 cgggcgcata gtttccggca agcagggctt tggccagcgg gttttcgatt tccgattgga
     4141 tcgcgcgttt gagcggacgt gcgccgtaaa tcgggtcgaa accggctttg gcgatgatgt
     4201 ccagagcggc atcggaaaca gtcaggcgca ggttttgttt ttccaaacgt ttttccaaac
     4261 ctttgagctg gattttcgca atactgcgga tattggcctg atccaagcca tggaacacga
     4321 ccacttcgtc gatgcggttg atcatttccg gacggaaatg ttctttcaca tcctccatca
     4381 caacttcttt caccgcttcg tagtcctgcg tgcccatttg ttggatgtgc tggctgccga
     4441 tgttggaagt catcacaatc acggtatttt taaaatctac cgtcctgcct tgtccgtcgg
     4501 tcaaacgacc gtcatccaat acttgcagca ggatgttgaa tacgtcggga tgggcttttt
     4561 ccacttcgtc caaaagaatc acgctgtacg gtttgcggcg gacttgttcg gtcaggtagc
     4621 cgccctcttc gtatccgaca taacccggag gcgcgccgat taagcgggca acggcgtgtt
     4681 tttccatgta ttcggacatg tcgatgcgga tgaggtggtc ttcgctgtcg aacaggaagc
     4741 ctgccaaagc tttgcacaac tcggtcttac ccacaccggt cgggcctaag aacaggaagc
     4801 tgccgtaagg tttgttcgga tcggcaaggc cggagcggct gcggcggatg gcatcggaca
     4861 cggcgcgcac ggcttcgttt tgaccgacta cgcggcggtg caacacttct tccattttca
     4921 acaatttatc gcgttcgcct tccatcattt tggatacggg aatgccggtc atacgggaaa
     4981 cgatttcggc cacttcatcc gcgccgacat tattacgcaa gagtttgttt gccggttttg
     5041 tgctgtccgt atctgcccgt tcggcggctg cacgctgttt ttccaaatgc tccaaatctt
     5101 catacatcaa ttttgaagcc agtgccaaat cgccttgccg ttttgcctgt tcgattttaa
     5161 ttttgacttc gtcaatttgt ttcttaatat tggcagcacc gtctgaaatc gctttttcgg
     5221 ctttccagat ttcgtccaaa tcggcgtatt ctttttgcaa gccgtcgatt tcctcgtcta
     5281 tcagttccaa acgttttttg ctggcatcgt ctttttcttt ttcaacgtgc gccttttcca
     5341 tccgaagctg aatcaaacgg cggtcgattt tgtccatagc ttccggtttg gtttcttttt
     5401 ccatcttgac acggctggca gcttcgtcaa tcaaatcaat cgccttgtcg ggcaaaaagc
     5461 ggtcggtaat gtagcggtcg ctcaactccg ctgcggcaac aatggcgggg tcggtaatat
     5521 cgatgccgtg gtggatttca tagcgttcct gcaaaccgcg caggatagcg atggtgtctt
     5581 ccacgctggg ttcgccgacc aatacttttt ggaaacggcg ttcgagtgcc gcgtcttttt
     5641 cgatgtattg gcggtattcg tccaaagtag tcgcaccgat acagtgcagt tcgccccgcg
     5701 ccaaggccgg tttcaacata ttgcccgcgt ccatcgcgcc gtcggttttg cccgcgccga
     5761 ccaaagtatg gatttcatcg atgaaaatca gggtgttgcc gtcgtctttc gccaaatcgt
     5821 tcaatacgcc tttcaagcgt tcttcaaatt cgccacggta tttcgcaccg gcaatcaaag
     5881 ccgccaaatc caaaaccagc aggcgtttgt tacgcaggga ttcaggtact tcgccgttga
     5941 cgatgcgttg cgccaaacct tcgacgatgg cggttttacc cacaccgggt tcgccaatca
     6001 gcacggggtt gtttttggtg cggcgttgca gcacctgaat cgcgcggcgg atttcatcat
     6061 cacgaccgat aacggggtcg agcttaccgt ctcgggcgcg ttgggtcaga tcgagcgtgt
     6121 attttttcaa agcatcgcgt tggtcttcgg cattcgcatc gttcacgttt tgtccgcctc
     6181 gtaccgcatc gatcgcggca ttgatgtttt gttcggtcgc accggcttct ttcaagattt
     6241 tgccggccgc atcgttctgc tgcaccaagg cgagcaggaa aagttcgctg gcgatatagg
     6301 catcgccgcg tttggttgcc gctttgtcca tcaggttcaa caccgcctgc aattcgcggc
     6361 tgggcagaat ctcaccacct tgtccggaca ctttcggcag gctattcaaa tgctgctgca
     6421 aacgctgttt aacctgcggc acgttcacgc ccgcatgagc caagagcgcg gcagctccgc
     6481 tgttttggtc gtcaagcagg gctttcagta cgaaacctgc ttccagatag ctgctgtccg
     6541 cagccaacgc caaactctga gcttctgcaa gggcttgttg gaatttggcg gttaatttgt
     6601 cgtatcgcat tttcgtttcc ttttcaaaat gtccgctgtc gaagcctata tgtgcatgac
     6661 tgtggataac tcaagtgttg ttttctgttt ttctatattt aattcgatat attgttgaat
     6721 ttaaagtata tagaaatgta tgataatatg tataactata tcttcttaat atggaaaagt
     6781 ctgttgtcgg ctggatgtat gtggcaaatc gggtataatc ggcgcatctt ttccctttca
     6841 gacggcattg ctgccgtaag gacattttta tgagcaaaaa acgagttctg accggcgtaa
     6901 ccaccaccgg caccccgcat ctgggcaact acgtcggcgc catccgcccc gccgtccgcg
     6961 cggcgcaaaa ccccgatacc gaatccttcc tcttcctcgc cgattaccac ggcatcatca
     7021 aatgccacga gcaggagatg attcaccaat ccacccaagc cgttgccgcc acttggcttg
     7081 cctgcggact cgaccccgag cgcaccacct tctaccgcca aagcgacatt cccgaagtga
     7141 tggagttgaa ctggattctg acctgcatca ccgccaaggg tttgatgaac cgcgcccacg
     7201 cctacaaagc cgccgttcaa gccaatgcgg aaaacgggca ggaagacccc gatttcggtg
     7261 tggaaatggg tttgttcagt tatccgattc tgatgactgc cgacattttg atgttcaacg
     7321 ccaacgaagt ccccgtcggg cgcgaccaaa tccaacacgt cgaaatggcg cgcgacattg
     7381 ccggccgctt caaccaccgt ttccaagaac tcttcaccct gcccgaagtg aaaatcgacg
     7441 aaaacgtcga actcttggtc ggtttggacg gacgcaaaat gtccaaatcc tacggcaata
     7501 ccattccgct ttgggaaaac gacaaaaaaa cccaaaaatc ggtcaacaaa atcattacca
     7561 acatgaaaga gccgggcgag ccgaaacagc ccgacgaaag ccctttgttt gaaatctata
     7621 aagccttctc cacgccgtct gaaacggcgg aatttacgca aatgcttgcc gacggcttgg
     7681 cttggggcga ggcgaaaaaa ctttcggcgg cgaaaatcaa cgccgaactg gcagaactgc
     7741 gcgaacgcta caacgcattg acctctaatc cttcgcaaat cgaagagatt ttgcaggcag
     7801 gcgcgcaaaa agcgcgcaaa gaagcgcgcg aattgttgga taaagtacgc gatgcggtcg
     7861 gcatccgccc gttgaaataa acccaatgcc gtccgaacct ccgcctgcca cgtttcagac
     7921 ggcatttcag acggcatttt gaaaccatca ggagtgcgga atgctgaaaa taccttttgc
     7981 cgtgttgggc ggctgcctgc tgcttgccgc ctgcggcaaa tccgaaaata cggcggaaca
     8041 gccgcaaaat gcggcacaaa gcgcgccgaa accggttttc aaagtcaaat acatcgacaa
     8101 tacggcgatt gccggtttgg ctttgggaca aagtagcgaa ggcaaaacca acgacggcaa
     8161 aaaacaaatc agttatccga ttaaaggctt gccggaacaa aacgccgtcc ggctgaccgg
     8221 aaagcatccc aacgacttgg aagccgtcgt cggcaaatgt atggaaaccg acggaaagga
     8281 cgcgccttcg ggctgggcgg aaaacggcgt gtgccatacc ttgtttgcca aactggtggg
     8341 caatatcgcc gaagacggcg gcaaactgac ggattacctg atttcgcatt ccgccctgca
     8401 accctatcag gcaggcaaaa gcggctatgc cgccgtgcag aacggacgct atgtgctgga
     8461 aatcgacagc gagggggcgt tttatttccg ccgccgccat tattgagtta ttcggacatc
     8521 acggaatata tttggatttt tcaaaccttg cggaaaaagt ccgcgccgcc gggaaactca
     8581 aaaggagggg atgtgtgtta caattttccg aactgttttc ataaaatagt tttcggacgt
     8641 gtttcaatat ggcattgatg ccgccttgtt gttcgagaaa aaacccttta tatttaatat
     8701 aatgggtttt aactaaacgg gaaaccgttt tctctccggc cgatgggcaa aatcagccga
     8761 ttgatggaac acggtccggt ttttaagcaa aaccttaatc aacaactcaa gaaggataag
     8821 acatgaaaaa attattgatt gccgcaatga tggcggctgc cttggcagct tgttcgcaag
     8881 aagccaaaca ggaggttaaa gaagcggccc aagccgttga gtccgatgtt aaagacactg
     8941 cggcttctgc cgccgagtct gccgcttctg ccgtcgaaga agcgaaaggc caagtcaaag
     9001 atgctgcggc tgatgcaaag gcaagtgccg aggaagctgt aactgaagcc aaagacgcgg
     9061 cagccgaaac caaagaagcg gtaagcgaag cggctaaaga cactttgaac aaagctgccg
     9121 acgcggctca ggaagcggca gacaaaatga aagacgccgc caaataattt gttgccttgg
     9181 caaaaatgac gagatgccgc ctgccggcaa cccaaatgaa accgcctgaa gattttcagg
     9241 cggtttttga gttgtatgcc ccttgttttt tacgcctgca tgaccgtgca gggaggatgt
     9301 gccgcgcata tcgggatatt gcggacaggg aaatgccgtc tgaacatcat ttagacggca
     9361 tttggtttgt caaggttcag acggcttcaa ctttgaacgg catattgatt ttttgccatt
     9421 ggacgctttc atattccaat gcgtcggcaa tcagggggtg gcgttccagc catttcctgt
     9481 caatacgcag gatgaagccg cagctttccg tatccgtacg caactgcata tttttcggga
     9541 aacccagatc ttggcgcgaa cggcagaaca gggcggcaag gcgcagggac aaaacggcat
     9601 accacaacat ttcgttgccg ccgataatgc cgcccatttt tttcatatcg ccgcgatgac
     9661 cgatgaccag ttgggcaagt atggtctgtt ccttgcgtga aaaacccggc atatcggcgt
     9721 tttcgaggat gtaggcggaa tgcttgtgat agccggtgtg ggcgatgtcc aaaccgattt
     9781 catgcagcgc ggcggcgcgt ccgagatact gttgccacaa ggcaagttct tgaaccgtaa
     9841 cgtttttggc atgacagagg ctgtccataa aggtttgcgc ggtctcggcg gtgcgtttcg
     9901 cctgattgag gctgacgtgg tagcggtgtt ggaactcggc aaccgtttgt ccgcgcatat
     9961 cttcgtttaa gccgcgcccg atcaaatcgt aaaacacgcc gtcgcgcagg gcggcttcgg
    10021 ttacggtcat cctgtcgagt ttcatttcct caaacgccgc catcatcacg gcaagtccgc
    10081 cggcaaaaac ttcgatgcgt tccggtttca ggttttcaaa tttggctttt ttgaccgaac
    10141 cggcttcgat gatgcgttcg gcgagggcgc gcattccctt gtaggtaatg tccgcctctt
    10201 ggggcatttc ggcggcaagc acgtcgcgga tggattttgc cgaacccgat gtgccgacgg
    10261 cgaaatccca accttcgcgc ctcatatttt tgctgatgcg ctggatttcg ttgcgggcgg
    10321 cggaaatggc ggcttggaag tctttggcgg tgattttgtt ttggaagaag cgcaggctgt
    10381 aggttacgca gcccaagggc aggctttcgg taatgtcggg attcagcgtc gaaccgatga
    10441 caaattctgt cgaactgccg ccgatgtcga taaccagcat tttgcccccg caggggggga
    10501 gggtgtggat cacgccggta taaatcagcc gcgcctcttc gcgcccggcg atgatttcga
    10561 tggggaaacc cagtgccgct tcggccttgg gaaggaaatc tgcgatgttt ttggcgacgc
    10621 ggaatgtatt ggttgccacg gcgcgcacct gctcggggcg gaagccgcgc aggcgttcgc
    10681 cgaattttgc caggcagtcc aaagcctgtt cctgcgagga tgtgctgagg tttttctgtt
    10741 cgtccagacc ggcggcaaaa cggaccatct gtttgaagga atcgatgact tttaattgtc
    10801 cgttgttgtt ttcgcaaatc tggaggcgga aactgttgga acccaaatcg acggaggcga
    10861 ggacgtttgc gggattggtg gtcatggcgg ataccggtgg gggaaaaacg caatgttact
    10921 ctgacggcgc aggcgttgac aataaatgat gcggctgttt ttgattctgc tcacggatgt
    10981 tgccgacggt atgtttccga cagcaaaaaa tgccgtctga aaaacgttcg gacggcattt
    11041 tttgcgctta tttgaaatcc ttttccacgc tcatgaaaat ctgcgtattt ttgcgtgtgt
    11101 aaaaactttt catattgctg tcgattttca gatagcggaa attgagttgc ggcgtaaagc
    11161 ccttccaaga gattttgtcg tgccgcaacg acaggtttgc ctgatattcg tggtctttgc
    11221 gcgggaagcg gtacacaatt gtcccgggtg catcaaaagt cctgcgggta tagcgcaggt
    11281 ttgcccgcag ccccaagccg ccgtcgaacg ttttgaccgc gccgacacgc aaacccttgc
    11341 ggacggaagc ctgttccgcc tctttcgtca tgtcgtgcgg ccagtccgca ccgccgtaaa
    11401 gcagccggtc tttcggcgcg gaatacatca gcgtcgcgcc cgccagcggc atatggctgc
    11461 cgtatcgggc ggcggtgcgg tcttcctgat aatgcttcca catattgccc gcgttcagcg
    11521 tcagccgcca gcgttcgccc aagcgtcggg agaaatcggc attgaagccg ccgacgaaat
    11581 tgtatcggct gccgcctaag aggttttgcc cgacaaacgg cacgatgccg aacgagcgcg
    11641 ttaccgaacg atttttatag ccgagcgaca ggtgcaggca ctgttcgctg aaatctttgt
    11701 tatcccaata atgcacgccg ccgccgctga tgccgccgta gaggaaatga tgcccgcccg
    11761 cattgatttc gcgcgacacg cctaagccct agcgcaaacc gtgtgccttt tgcggcaggc
    11821 tgtcggcggt tttcgtccag cttctgcccg caaattcaat cgttttttcg gacgaagcgt
    11881 t
//
LOCUS       DBJGGI010000063        11841 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.063, whole genome shotgun sequence.
ACCESSION   DBJGGI010000063 DBJGGI010000000
VERSION     DBJGGI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11841
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.063"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(<1..138)
                     /locus_tag="ACSIGJ_001685"
     CDS             complement(<1..138)
                     /locus_tag="ACSIGJ_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HHQ1330507.1"
                     /translation="MKITHCKLKKEVQKEPLRSFVPEVTARSAADILGIHPDSAALFY
                     RK"
     gene            471..1325
                     /locus_tag="ACSIGJ_001686"
     CDS             471..1325
                     /locus_tag="ACSIGJ_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin-like passenger domain-containing
                     protein"
                     /protein_id="HHQ1330508.1"
                     /translation="MSNGARWTVTNDSMLKELDLSEDAQVEFSDNNKFVKVSVSKLKG
                     DGGVFKMYGDIVKGESDKLITRKGSEGTHIIEYMDDAKAKTTGREYLKLVENKGNQED
                     NKASNKASYKLNVRCTEQGGWCFALGESGASKKVNISTDGKRDFYLYPDTLTPGASSS
                     VLFGEALYQLNAVSDETLVQRMGEIHADGTPQEDNNVWIKRVGGKFSGSRSDYRVGGY
                     GNRYWGFAGGFNRTGFGDKWIHYKGLMLRHLQSSYASEDYVGSGKILRQGGRCLFRLA
                     QPGKQGLL"
     gene            1415..1873
                     /locus_tag="ACSIGJ_001687"
     CDS             1415..1873
                     /locus_tag="ACSIGJ_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HHQ1330509.1"
                     /translation="MPICFPPKPVGGWKNKTAAKTYRWQPEVQLSYWFTRGYGFPLSN
                     GLSAETDNFRSLMGRFGFRAGVDGLDGGRLNIYGKLMYKREFIGTIRHRFNGSAVEEF
                     KHRGGWLEYGLGVVRRNAGNGRQLYFEAQRSSMHTMRQNWQVNMGVRSMF"
     gene            complement(2356..3768)
                     /gene="trkA"
                     /locus_tag="ACSIGJ_001688"
     CDS             complement(2356..3768)
                     /gene="trkA"
                     /locus_tag="ACSIGJ_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HHQ1330510.1"
                     /translation="MKILILGNGQVGSTVAQNLAAITNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(3866..5388)
                     /locus_tag="ACSIGJ_001689"
                     /pseudo
     CDS             complement(3866..5388)
                     /locus_tag="ACSIGJ_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
     gene            complement(5695..6501)
                     /locus_tag="ACSIGJ_001690"
     CDS             complement(5695..6501)
                     /locus_tag="ACSIGJ_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HHQ1330511.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPGKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKVSSSEDLKKMNKVGVVTGHTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPAKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEGGQAAK"
     gene            complement(6553..6804)
                     /locus_tag="ACSIGJ_001691"
     CDS             complement(6553..6804)
                     /locus_tag="ACSIGJ_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO1151 family protein"
                     /protein_id="HHQ1330512.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPADTAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            6906..7676
                     /locus_tag="ACSIGJ_001692"
     CDS             6906..7676
                     /locus_tag="ACSIGJ_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330513.1"
                     /translation="MDTNNQDKEPNWEKRLENLRQTRRMNTAPAYVPLTKPSEVGKEE
                     PIPKLDDANRDRVLKAYLKKWQEEHNSAVADCGEEVETDPMIVLQENWLNAQASLQMP
                     VSEKHIESRRRIRFDAKKDSAEFETAQIEGGENADAAESGTAADGVAENGEGGAEEAL
                     MPVQINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGMIRTAVQKQMALFT
                     YQLQQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            7822..8991
                     /gene="metZ"
                     /locus_tag="ACSIGJ_001693"
     CDS             7822..8991
                     /gene="metZ"
                     /locus_tag="ACSIGJ_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HHQ1330514.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTIAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGGH
                     VVSSRSLFGTTVGFINNIVTKFGIGVSRVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALRIAQWLQE
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITNPWTTTHGRMQPEEKLAAGIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            9086..9292
                     /locus_tag="ACSIGJ_001694"
     CDS             9086..9292
                     /locus_tag="ACSIGJ_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HHQ1330515.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAVLF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            9688..9912
                     /locus_tag="ACSIGJ_001695"
     CDS             9688..9912
                     /locus_tag="ACSIGJ_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330516.1"
                     /translation="MFIFYTVNPEHVYFPKAYIMKVFKDKGYESQCITTVSFYICNPT
                     LKQKTENEAYEYGRLFVKELMHKECNRESL"
     gene            9941..11227
                     /locus_tag="ACSIGJ_001696"
     CDS             9941..11227
                     /locus_tag="ACSIGJ_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HHQ1330517.1"
                     /translation="MADALADGGAAATAVGCPPRLIGGEQNKTPNPKGAEKTENQEFQ
                     FEYFSHFVSDGKGRFIEIPLRRGRDDGAFIDQITFTIHENSMTKVTGKGLVSDTEFVV
                     RYSELLEEILGFGITKKLPFKGKFFYQSCYQFGPDNVEYGKVHYGGQCETMLVELNGT
                     GCMAALPGWENRLYEFLSKCVRPKITRIDVAHDFFNGEYTPDQAMLDHDNGHYDVHNM
                     RPKSECRGTAWRNEDGSGKTFYIGKRENSKFTRVYEKGRQFGDVDSPWVRFEGDIEIP
                     LDVLLYSGSYLGGAYPICKEIFKTEAKRMEVKVKNVNLIFDVKLFHARNQVGKMVNFL
                     RDIGWDDSRIVDELVKGVEGYPKGLQPEQYDCKNQTQKFQYIHEEQKAINALNIETLF
                     DDLIEEREYAFPQDRERQHIKDIEYEERQISDFLNN"
     gene            11241..11552
                     /locus_tag="ACSIGJ_001697"
     CDS             11241..11552
                     /locus_tag="ACSIGJ_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330518.1"
                     /translation="MFETSQVTTYTATLLGAKKFKGEIDGNKIDSCTVLVASPMPSNG
                     NAVGFTSESMKFGDSHNFEKLKNLKFPCAVDLTVAMESTGKGLVQKLLDFQVKGAAPK
                     A"
     gene            11566..11769
                     /locus_tag="ACSIGJ_001698"
     CDS             11566..11769
                     /locus_tag="ACSIGJ_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330519.1"
                     /translation="MSRYQQKFIVQELENHEFIYPDSFGDIGFTSNIKSAGKYDSYED
                     AFSSALEEIGGEFVIFGFYEKED"
BASE COUNT         2929 a         2789 c         3315 g         2808 t
ORIGIN      
        1 tttacggtaa aacagtgccg ccgaatcggg atggataccc aaaatatcgg cggcagaacg
       61 ggcggtaact tccggtacaa aagaacggag cggttctttt tgtacttctt tctttaattt
      121 gcagtgtgtt atcttcatat ttcgagggta acatatctgc taatctggta cagacccgaa
      181 tgttaagcag aaacgattgt ctcaaaaaaa gcaactttgg ataaatacaa tcaagaattg
      241 gctgaactag gtgaaaatga aaagaaaaaa gaagaactag aaaaaaagta aaacagcaag
      301 aaaacaaaat tcagatcaga aaaaggcaca tagaacgtct aaaagaagac atagaaaagg
      361 ttgcggtact cgaagaagaa atcaaaagat ctgaaaagtt cttcgatgaa aacggttggc
      421 ttaaagacag cattatcgac gacaaaacaa aaaacgccgt caacctcaaa atgagcaacg
      481 gcgcgcgttg gacggttacc aatgattcaa tgctgaaaga gttggatttg tcggaagacg
      541 cgcaggtcga atttagcgac aacaataaat tcgtcaaagt gtccgtgagc aagctcaagg
      601 gcgatggcgg cgtgttcaaa atgtatggcg acatcgtcaa aggagaatcg gacaagctga
      661 ttacccgaaa aggcagcgaa gggacgcaca tcatcgaata tatggacgat gccaaggcaa
      721 aaacgacggg aagggaatat ctgaaactgg tcgaaaacaa gggcaatcaa gaagacaata
      781 aagcatcaaa taaagcatca tacaaattga atgtccgctg tacggaacag ggagggtggt
      841 gttttgcctt gggtgagtcg ggtgcttcca aaaaggtcaa tatttccaca gacggtaagc
      901 gggacttcta cctctacccg gataccctga cgccgggcgc atcgagcagc gtccttttcg
      961 gcgaggcgtt gtatcagttg aacgcggttt ccgatgaaac gctggtgcag cgtatgggcg
     1021 aaatccacgc cgacggaacg ccgcaggaag acaacaacgt ttggatcaaa cgcgtcggcg
     1081 gaaaattctc cggcagccgc agcgattacc gcgtgggcgg ttacggcaac cgctattggg
     1141 gctttgccgg cggcttcaac aggacggggt tcggcgataa atggattcat tacaaagggc
     1201 tgatgctccg ccacctccaa tcgtcctacg cttccgaaga ctacgtcggc agcggcaaaa
     1261 tcttacggca gggcggcagg tgtttattcc gcctggctca accgggaaag cagggcttat
     1321 tatgatttgg tcgccaacat cgcccgatat aagggcagct acgggctgac caattacgcc
     1381 ggcaaacggg tcgaatcgga cgaggcgcgc ctgaatgcct atatgctttc cgccgaagcc
     1441 ggtaggcgga tggaaaaaca agacggcggc aaaaacctat cggtggcagc cggaagtgca
     1501 gttgtcttac tggtttacgc gcggctacgg ctttccgctg tcaaacgggc tgtctgccga
     1561 gacggacaac ttccgcagcc tgatggggcg gttcggcttt cgggcgggcg tggacggttt
     1621 ggacggcggc aggctgaaca tctacggcaa gctgatgtac aaacgcgagt ttatcggcac
     1681 gatccgccac aggttcaacg gctctgccgt ggaggaattc aaacaccggg gcggctggtt
     1741 ggaatacggc ttgggcgtgg tgcgccgcaa tgccggaaac ggacggcagc tttattttga
     1801 ggcgcaaagg tcttcgatgc acacgatgcg ccagaattgg caggtcaata tgggcgtgcg
     1861 cagtatgttc tgatgcgcgg atacgcctga aaacaatccg gaacggtcag acgcgttccg
     1921 gattgttttt ttgtcggggc ggtttgctgc catatcaaaa caggcgggcg atgccgtctg
     1981 aaacttcgtc attcccgcga aagcgggaat ctagaacctc aaactttcag ataatctttg
     2041 aatattgctg ttgctcaatg gtttggattc ccgcaacctt tcgtcattcc cgtgaaaacg
     2101 ggaatctaga atctctaaag cttcagctaa cctttgaata ttgccgcttt ccaatggtct
     2161 agattcccgc ctgcacggga atgacgattt ggaaattacc cgaaacccaa aaacaactga
     2221 aaccgaacag gccggattcc cgctacgcgg gaatgacggg gggcggatgc cgtctgaagc
     2281 ggtgggctga agcccactct actattattt ttgcttccat accgcttggc ggcaatgtgt
     2341 ccggaatgga gcggtttatc cgaaaaagcc cattttgact tggatgagtt tctccagttc
     2401 gttcaggatg cgccggcgcg agacgaaaaa gatgatgtgg tcaccgtctt ggatgacggt
     2461 ttcggtatgg tgtcccataa tggtttcgcc ggttccggcg cggacgacgg cggcaatgtg
     2521 gcagccttcg ggccatttga tgccgctgat gcgcctgccg atgatggcgg aagttttttt
     2581 gtcgccgtgc gcgacgactt cgatggcttc cgccgtgccg cgccggatgg ggtggacggc
     2641 aacgatgtcg ccgcgccgga tgtgggcgag tatcgagccg atggtgatga ggtgggggga
     2701 gacgacgatg tcgattttgt tgccttcgag caaatcgacg tagcttgagc ggttgacgat
     2761 gccgatgacg cgcttcgcgc cgaggttttt cgccaaaagg gcggacataa tgttgctttc
     2821 gtcgtcgttg gtcagggcgc agaatacgtc gatttcgtcg atgtattcgt tgtcgagcag
     2881 ggtttcgtcg gttgccgaac cttgcaggac gagggtgttg tcgaggtttt cggctatcca
     2941 ttcggcacgg cgcggccggc attcgatgat tttgacgttg tatgcgtgtt cgagctgctt
     3001 ggcgaggcgg tagccgatgt tgccgccgcc ggcaatcatg atgcggcggg tgctggtttc
     3061 tttggggcgc aattcgggta tgaccgcccc gatgttttcg gcggcggcgg caaacaggat
     3121 ttcgtcgcct tcgatgatga cggtttgcgg cgcggggacg atgaggcggt tgttgcggta
     3181 aacggcgcag atttggcagt cggccccgtc gggcaaatct tgggcgatgt cggcaatgct
     3241 gcgtccgaca agcagcccgc cgcgccgcgc ctgtatgatg accatccgca cgcggtcgtc
     3301 tgcaaaacgt aaaacctgca atgcgcccgg gcagtctatc aggccggcaa gctgttcggt
     3361 aacgagctgt tcggggctga tggtttcggt tatgccgaat atggaaaggc tgccgttttc
     3421 gttgttttcg agcttggggc tgaggtattc gaggtattcg ctggaacgga cgcgcgcgat
     3481 gcggccgggg atgttgaaca ggtcggcggc aactttgcag gcgacgatgt tggtttcgtc
     3541 gctgcgggag agcgcgagca gcaagtcggc atcttccgcg ccggcgcgtt ctaatgtgaa
     3601 gggggatgcg ccgttgccga aaacggtttg gacatcgagg cggctgcctg tttcctgcaa
     3661 tgctttttcg tcgatgtcga taacggttac gtcgttgttg gtgatggcgg caaggttttg
     3721 cgcgacggta gaacctacct gcccgttgcc taaaatgagg attttcacgg tatgggtcgc
     3781 cgggtgatga aatggtggat tgtaccgcaa ccggcggcgg aaatgccgtc tgaaggcaga
     3841 ttttgcgttc agacggcatt gcgcttcaag actcgacggg gatgatgccg attttcgcct
     3901 gccatttgcg cggggcggtg gcgtggatgg attcgccttt gctgtccacg gcgacggtta
     3961 cgggcatatc tttgacttca aattcgtaaa cggcttccat acccaattcg gggaacgcca
     4021 agactttgga agatttgatg gcttttgcca cgaggtatgc cgcgccgccg actgccatga
     4081 ggtacacggc cttgttgtcg gcgatggctt cgcaggtggc cgcgccgcgc tcggatttgc
     4141 cgatcatgcc caagaggccg gtttgtttga gcatttggcg ggtaaatttg tccatgcggg
     4201 tggctgtggt cggacctgcg ggaccgacga cttcatcgcc gaccggatcg acggggccga
     4261 cgtagtaaat caggcggttg gtgaaatcga cgggcaactc ctcgcctttg tcgagcatat
     4321 tgacgaggcg tttgtgcgcg gcatcgcggc cggtgaggat tttgccgttc aacagcaata
     4381 cgtcgccggt tttccagctt gccacttctt ctttggtcag cttatcgaca tcgacgcgtt
     4441 tgccgttgtc ggggctgtaa gtcagatcgg gtcagtcttc gacgcgcggc ggcgtgagtt
     4501 cgacaggacc tgagccgtcc aattcaaatt cgacgtggcg ggtggcggca cagttgggaa
     4561 tcatggcaat cggtttggag gcggcatggg tcgggtaatc gaggattttc acgtccaaca
     4621 cggtggtcag accgcccaag ccttgcgcgc cgatgcccag cgcgttgacc ttttcaaaga
     4681 gttcgaggcg cagggcttcg gtggtggaca attccgcgcc ggacgcggct ttttcctgca
     4741 attcttggat gtcgatgtgg ctcatcaggg attctttcgc catcaacacg gctttttcgg
     4801 gcgtgccgcc gatgccgatg cccaagatgc cgggaggaca ccagcccgcg cccatcgtcg
     4861 ggatggtttt caatacccaa tcgacgatgt tgtcggaagg gttgagcata ggagtttgga
     4921 tttgttttca gagccgccgc cttttgccgc gcaggttact tcgactttac cgcccggcac
     4981 gatgctcatg tggatgacgg cgggggtgtt gtctttggtg ttttggcgtt tgccggccgg
     5041 atcggcgagg acggaagcgc gcagggtgtt gccttcccaa gtgtaggcgc ggcgtacgcc
     5101 ttcgttaacc atctcttcca cgctcatgtc cgcatcccat tgcacatcca taccgacttt
     5161 gaggaagacg gttgcgatac ctgtgtcttg gcagatgggg cggttgtttt cggcacacat
     5221 acggctgttg accaaaatct gcgtcatcgc gtctttggcg gcgggatttt cttccttctg
     5281 ccacgcctta taaagcgcgt cgatgtagtc ttttggatgg tagtagctga tgaattggaa
     5341 ggcatcgcag atactttgaa taaagtcttc ttgcttgata acggtcatga ttgtgttcct
     5401 tttcaaggtt gtggcggtaa gtgtctgctg ctgttttaaa caaccttttt gaaggctgtt
     5461 ttttatcagg ttgaaatagt gccagcgttt gtacggtgaa gacaatacat aattctaatc
     5521 tcttgattat caatttgttt taataaaata ctgttttatt cggattttga tatctgtaat
     5581 ctttgcctgt tgctttgcgg tagggagaaa tcagcaggca gttcattgtg gaccggttga
     5641 tgaacaatgc cgtctgaaag ggcttcagac ggcattttgt tcggacgggc gggcttattt
     5701 cgcagcctgt ccgccctctt tggcaaaata tttggcgtag atcttgtcgt attcgccgct
     5761 ttcgcgtact ttttccaacg catcgttcag cattttgacg gttgcttcgt cgcctttgcg
     5821 taccgcgatg ccgtagtgtt cggtggtgaa gtcgggcagg gtaacgaagt ccattccttt
     5881 ggccgggttg tttttcacat aattggcgat gaccgcgctg tcgctgacca cggaatccaa
     5941 gccgccgttt tccagttctt tgataatcag ggggacgttt tcgaagcgcg cgattttcgg
     6001 attgtcgttg cccaagagtt tggaaacgga gaaatcgccc gtgtggccgg taaccacgcc
     6061 gactttgttc atctttttca aatcttcgga agaagatact tttttgcctt tcggaacgag
     6121 gacgacttgg gtgatttcaa aatacgggtc gctgaaatcc atagactgtt tgcggtcgtc
     6181 ggtaatggtt acgcccgaca tcacaacgtc cgcatcgccg ttgttcaagg cggggaaaag
     6241 gctgtcccac ggctggtgtt tgaattcgat tttaaaattg cccgccttcg ccatcgcgtt
     6301 catcaaatcc acgtcgaaac cttcgacatt gcctttcgag tctaaagatt caaagggggc
     6361 aaactcggcg ttggaagcca cgcggtacac tttgccgggg ttggcggcag gcgcggcggc
     6421 atctttgccc tgaccgccgc aggcagacag cgcgagtgcg gaacaggcaa gggcggcggc
     6481 aatccatttt ttcatattca tcaaaattcc tttcggtaat caaaaagcct aaaaaatcag
     6541 tttaacataa aattaacgtt ccttttcacg gaaaaacgca taagtaacgt cgtggaacaa
     6601 atccgtatgc gggctgtcct catagctcaa ttctgccaag gcgtcctcaa aagcaagctg
     6661 aatcgactcg accgcgattt cggcggtgtc tgcgggaagg gcgcggatca gggctttgaa
     6721 ggcggtggac aggacgtggt tctgcatacg cagcacatcg tttgcttctt ccagatattc
     6781 caaacgctgt tcgatgctgc ccatgatgta tcctcgcgaa tctgttaaaa tgcgccatta
     6841 tatcaaactg cgctttgggc gcgggtttgc ccgcctgcaa agattaagaa ccggaagccg
     6901 aaaaaatgga tacaaacaat caggataaag agccgaattg ggaaaaacgc ctggaaaacc
     6961 tgcgtcagac gcgccgtatg aataccgcgc ctgcctatgt gccgctgacc aaaccttctg
     7021 aagtcggaaa ggaagagccc ataccgaaat tggacgatgc aaaccgagac cgtgtgttaa
     7081 aggcgtatct gaagaaatgg caggaagaac acaacagcgc cgttgccgat tgcggcgagg
     7141 aagtcgaaac cgacccgatg atcgtccttc aggaaaactg gctcaacgcg caggcttccc
     7201 tgcaaatgcc ggtttcggaa aaacacatcg aatcgcggcg gagaatccgg ttcgatgcca
     7261 aaaaagacag tgccgaattt gaaaccgcac agattgaggg cggggaaaac gccgatgcgg
     7321 cagaatccgg tacggcggca gacggggttg cggaaaacgg cgaaggcggt gcggaagagg
     7381 cgttgatgcc cgtccaaatc aatatcctga acccgaaagc cgtcaaccgg cgcgaagtgt
     7441 tctgcctgtc cgaacaggag ctgaccgaac ggttgatcaa acgcctgcgc ccgcatctga
     7501 ccgataccgt caacggcatg atccgtacgg cggtgcagaa gcagatggcg ctctttactt
     7561 atcagctcca gcaaatgctg cacgagcagg cgggtgcggt agtcgaagac gtgttggaac
     7621 acgatgtccg taaaatcctc aacgacatca agtacgaatt aaaatacaaa cgctgattgc
     7681 catgccgtct gaaagccgtg ttcagacggc attttcctgc ccgtccgact tgaaagcctc
     7741 atggaacagg cgcaaaatat taataaatta aacaacgggt gcacgccccg ttgccgaacg
     7801 gacaaaaccg aggaatcacc gatgagcaaa aaacttcatc cgcaaacgct cgccatacgc
     7861 ggcggcaaag aacaaaccgg ataccgcgaa cacaatcagg cattgttcct gaccagcagc
     7921 ttcatgtggg acaacgccca acacgctgcc gatttgtttt caaaaaaaat caaaggcttc
     7981 acttataccc gtaccgccaa cccgaccatc gcggcctttg aaaaacgcat agccgctttg
     8041 gaaggcgcgg aacgcgcggt cgccacttcg accggcatgt ccgcgattca ggcggcgttt
     8101 ttcaccttcc tgcaggcggg cggccatgtg gtttccagcc gcagcctgtt cggcacgacc
     8161 gtcggcttta tcaataacat cgttaccaaa ttcggcatcg gagtgagccg cgtgtcgccg
     8221 actgatataa atgaatggaa agccgccgtc aaagccaaca ccaaactgct gtttttggaa
     8281 acgccgtcca accccttggg cgaagtggcc gacttggaag ccttggcgga attggcgcac
     8341 ggcatcggcg cgctcttggt ggtggacaac agcctgctgt cgcccgtcgg ctcgcagcct
     8401 ttgaaacacg gcgcggatat ttccgtttcc tccgccacca aagccataga cggacacggg
     8461 cgcgtgatgg gcggcgtgtt ggcgggttcg gaagaactga tggcgcaggt cgccgtttac
     8521 tgcaactctt gcggtttggc gatgtcgccg tttaacgcgt ggcagttgtt gagcggcgtg
     8581 gaaaccctgt cgctgcgtat ggaaaaacag tttgacaacg ccttgagaat cgcgcaatgg
     8641 ctgcaagagc agccgcaggt tcaagcagtg tactacaccg gattgcccga ccatccgcag
     8701 gcggagctta tccgcaaaca gcaaaacggc ggcggcatcg tcatcggttt tgaagtcgcc
     8761 gaccaagcag ccgcgtggaa agtcgtggac ggcgtggaac tcttttcccg taccgccaac
     8821 ctcggcgacg tgcgctcgac catcaccaat ccgtggacaa ccacgcacgg caggatgcag
     8881 cccgaagaaa aactcgccgc cggtatccgc cccggactgg tgcgcctgtc ggtcggtttg
     8941 gaatatgttg gcgatttgat tgacgatttg aaacaggcac tcgcgcgctg attgcgtgtt
     9001 tcagacggca tcggcggaaa aatgcggcag atgccgtctg aaacccttat tgcgtatggc
     9061 atacacatca acaataggaa aaaatatgga tgaagcggtt tttgccgatt gggcgttgaa
     9121 aatctgcctg accggtttga ttattttttt gggttttatc gtttggaatt tgggcaagga
     9181 gtccaaagca ggcaaattcg gcatcgccgt tttgtttttg gttttgggct tgggtgtgtt
     9241 cggttttgtg ttcaaagagt tattgattaa gtttttggta ttgcccaaat aggtttgccg
     9301 tctgtcgaaa tgccgtctga aatcataaaa agtaatctgc accctaaaag tttgatttaa
     9361 ccgccaactg attaaggtgc gggtttttat gacaaaataa aaatcatctt tccgcaatca
     9421 ttcccacgga tgcgaaagcc tgtctttttg aatcccggat aattttacat cgtcattccc
     9481 gcgcaggcgg gaatctagtc cgttcggttt cagtcattta cgataaattc ctgttgcctt
     9541 tcatttccgg attcccactt tcgcgggaat gacggaggag cggataaatt ctcgcaatct
     9601 aaaaccttat atattgcgct ccataagggg cgcatatttt taatccttgt aaagcccctt
     9661 aactctagtc agtgaagggg cttaatcatg tttattttct atactgtcaa tcccgaacac
     9721 gtatatttcc ccaaagcata catcatgaaa gtgttcaagg ataaaggcta cgaatcccaa
     9781 tgcatcacaa cagtttcttt ttatatctgc aatccgactt tgaagcaaaa gaccgaaaac
     9841 gaagcgtatg aatacggcag attgtttgtt aaagaactga tgcataagga atgcaaccgt
     9901 gaaagccttt gaagataaag ccttggcggt gtccgcggac atggcggacg cgttagcgga
     9961 cggcggggct gcggcaaccg ccgtaggctg cccccctagg ctaatagggg gggagcaaaa
    10021 taaaacccca aatccgaagg gtgcggaaaa aacagaaaac caagaatttc aattcgagta
    10081 tttcagtcat tttgtatcgg acggcaaagg cagatttata gaaataccgt taaggcgtgg
    10141 cagggatgac ggagcgttca tagaccaaat cactttcacg atacatgaaa attccatgac
    10201 gaaagtaacc ggaaagggat tggtatcaga tacggaattc gttgtccggt atagcgagct
    10261 gcttgaagaa atcttgggat tcggcattac caaaaaatta ccgtttaaag gaaagttttt
    10321 ctatcaaagc tgttatcaat tcgggcccga taacgtcgaa tacggaaagg ttcattacgg
    10381 cggccaatgt gaaacgatgt tggttgaatt gaacggtaca ggctgcatgg ctgctttacc
    10441 cggttgggaa aaccgattgt atgaattttt aagtaaatgc gtccgaccca aaataacccg
    10501 tatcgatgtc gcccatgatt ttttcaacgg agagtacaca cccgatcagg caatgttaga
    10561 ccacgataac ggacattacg acgtacacaa tatgcggcca aaaagcgaat gtcgcggcac
    10621 ggcatggcgc aatgaagacg gcagtggaaa aactttttac atcggcaagc gcgaaaattc
    10681 aaaatttacc cgtgtttacg agaaaggcag acagtttgga gatgttgata gcccttgggt
    10741 caggtttgag ggggatatag aaataccctt ggatgtccta ctttattcgg ggtcgtatct
    10801 aggcggcgca tatccgattt gcaaggagat ttttaagaca gaagccaagc gcatggaagt
    10861 aaaagttaaa aacgttaatc tgatattcga cgttaagctt tttcatgcaa ggaatcaagt
    10921 cggcaagatg gttaatttcc tccgcgatat agggtgggac gatagcagga tagttgatga
    10981 gctggtgaaa ggcgttgaag gttatccaaa aggtttacag cctgagcaat acgattgcaa
    11041 aaaccaaacg cagaaattcc agtatatcca cgaagaacag aaagccatta acgcattgaa
    11101 cattgaaaca ctgtttgatg atttgattga agagagagaa tacgcatttc ctcaagacag
    11161 ggaacggcaa cacatcaagg atattgagta cgaggaacgt caaatatccg attttttaaa
    11221 caactgaagg aaattgaaaa atgtttgaaa caagccaagt aaccacatat accgccactt
    11281 tgttgggcgc aaaaaaattc aaaggcgaaa tcgacggtaa caaaatcgat tcatgcaccg
    11341 tcttggtagc cagccccatg ccgtcaaacg gcaatgccgt gggctttacc tcggaaagca
    11401 tgaagtttgg tgacagccat aattttgaaa aattgaaaaa ccttaaattc ccctgtgcgg
    11461 ttgatctaac cgttgccatg gaatcaacag gaaaaggctt agtgcagaaa ttgttggatt
    11521 tccaagtaaa gggcgcagca cctaaagcct aagaaaggct gaatcatgag cagataccaa
    11581 cagaaattta tcgtacagga attggaaaat cacgaattca tctatcccga ttcgtttggc
    11641 gatattggat ttacgtcgaa catcaaatca gccggtaaat atgacagcta tgaagacgct
    11701 tttagctcgg cattggaaga gataggcggg gaatttgtaa ttttcggatt ctacgaaaaa
    11761 gaagattaag tttaagaggc tcggcaggcg gtctctaaaa ccttcacaaa gcccgcaaac
    11821 acattttttt aaaaatttcg t
//
LOCUS       DBJGGI010000064        11434 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.064, whole genome shotgun sequence.
ACCESSION   DBJGGI010000064 DBJGGI010000000
VERSION     DBJGGI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11434
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.064"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(168..1247)
                     /gene="apbC"
                     /locus_tag="ACSIGJ_001699"
     CDS             complement(168..1247)
                     /gene="apbC"
                     /locus_tag="ACSIGJ_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HHQ1330520.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVGQRSDGIHIALHF
                     GFPVAHIAAALADAVQETLMPETGGAHIHLSIDTEIGTHKVRPGVATIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVHDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTGQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPARLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
     gene            1466..1541
                     /locus_tag="ACSIGJ_001700"
     tRNA            1466..1541
                     /locus_tag="ACSIGJ_001700"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1499..1501,aa:Lys,seq:ttt)
     gene            1614..2123
                     /locus_tag="ACSIGJ_001701"
     CDS             1614..2123
                     /locus_tag="ACSIGJ_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HHQ1330521.1"
                     /translation="MKKKLLSGIKFAVRTALVFLLVSLFLDWVRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDRTVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVSDEDGGLARSWRIAATPAVVLVKNGKMVRYTTGISSYWGLRA
                     RIFQADFFG"
     gene            2464..2904
                     /gene="nadR"
                     /locus_tag="ACSIGJ_001702"
     CDS             2464..2904
                     /gene="nadR"
                     /locus_tag="ACSIGJ_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HHQ1330522.1"
                     /translation="MPTQSKHASINIGLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVYLKLTSEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            3019..3519
                     /gene="hpaC"
                     /locus_tag="ACSIGJ_001703"
     CDS             3019..3519
                     /gene="hpaC"
                     /locus_tag="ACSIGJ_001703"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042537 - benzene-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HHQ1330523.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINMLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRPLD"
     gene            3535..4230
                     /gene="murU"
                     /locus_tag="ACSIGJ_001704"
     CDS             3535..4230
                     /gene="murU"
                     /locus_tag="ACSIGJ_001704"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HHQ1330524.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIENALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVANGDVLTDIDFTAAFQTASSLPGHISAHLWLVGNPPHNPDGDFSLLPDGSVRPEVS
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLLNEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAAAWK"
     gene            complement(4264..4694)
                     /locus_tag="ACSIGJ_001705"
                     /pseudo
     CDS             complement(4264..4694)
                     /locus_tag="ACSIGJ_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            4869..6545
                     /locus_tag="ACSIGJ_001706"
     CDS             4869..6545
                     /locus_tag="ACSIGJ_001706"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HHQ1330525.1"
                     /translation="MSFKTDAETAQSSTMRPIGEIAAKLGLNVDNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDSVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RFGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPVFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIDNQPNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RITVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            6659..7750
                     /gene="ychF"
                     /locus_tag="ACSIGJ_001707"
     CDS             6659..7750
                     /gene="ychF"
                     /locus_tag="ACSIGJ_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HHQ1330526.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNRFLANIRETDAIVN
                     VVRCFDDDNIVHVSGKVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERALLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDGEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVIAYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            7788..8027
                     /locus_tag="ACSIGJ_001708"
     CDS             7788..8027
                     /locus_tag="ACSIGJ_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330527.1"
                     /translation="MPSEAGFGGFRRSHTAGMQPHQNKIPPAFPICPPRFLQNKPPAL
                     PLREAVRYSEYPEPRYKMTFQTAFAQPAAFQVKTL"
     gene            8024..9898
                     /locus_tag="ACSIGJ_001709"
     CDS             8024..9898
                     /locus_tag="ACSIGJ_001709"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HHQ1330528.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKDWLPGGFLGVDIFFV
                     ISGFLMTAILLREMSGGRFFLKTFYIRRIKRILPAFFAVLAATLAGGFFLFTKDDFFL
                     LWKSALTALGFASNLYFARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVAR
                     KSLRVQFGFLAALCALSLAASFMPSALDKYYLPHLRACEMLVGSLTAVRMRYRQQRNP
                     AVGKRYAAVGALFSACILSACLFAYSEQTAYFPGSAALIPCLAVAALIYFNHYEHPLK
                     KFFQWKITVAAGLISYSLYLWHWPILAFMRYIGPDNLPPYSPAAAIVLTLAFSLISYH
                     CIEKPFKKWKGSFAQSVLWIYALPMLVLGAGSFFAMRLPFMAQYDRLGLTRSNTSCHN
                     NTGKQCLWGDTEKQPELLVLGDSHADHYKTFFDAVGKKEKWSATMVSADACAYVEGYA
                     SRVFQNWAACRAVYRYAEEHLPRYPKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRML
                     HKLSSEKQAVYLMADNLASSYNVQRAYILSSRIPGCRQTLRPDDESTLKANARIRELA
                     AKYPNVYIIDAAAYIPADFQIGGLPVYSDKDHINPYGGTELAKRFSEKQRFLDTRHNH
                     "
     gene            9960..11255
                     /gene="tyrS"
                     /locus_tag="ACSIGJ_001710"
     CDS             9960..11255
                     /gene="tyrS"
                     /locus_tag="ACSIGJ_001710"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HHQ1330529.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVGSIR
                     SQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIGVVEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSRLTES
                     DFEQLALDGLPAFEVSDGINAVEALVKTGLAASNKEARGFVNAKAVLLNGKPAEANNP
                     NHAAERPDDAYLLIGEYKRFGKYTILRRGKRNHALLVWK"
BASE COUNT         2533 a         3144 c         3179 g         2578 t
ORIGIN      
        1 cattctccaa gttgcggcaa cctttcccaa caacttaacc gcccaaatac aagccttgcg
       61 gcttgctgcc ctctctccag ccctctccca cgggagagag gactatgaag cccgtcggca
      121 ggcgcggggc ttgggacggc attgctgttg cggtttcggg cgcggcttta ttcgacgaca
      181 attttaggga accggctgct gaagtctttg cctttgtcgg caatgcccag ggcgatttgg
      241 aatgcggcat cggtgtagat tcgggcgatg gcggggtgtt cgtcgaacag tcgcgccggt
      301 gtgccgccgt ccatggcttc gcgcacgggc aggcttaggg gaagctgtcc gagcaggggg
      361 acgttgaggc gggccgccaa atctttgccg ccgtccgtgc cgaacagtgc ttcgctatgc
      421 ccgcagttgg agcagatgtg gacggacatg ttttccaata cgcctaaaat gggaatgttg
      481 accttgcgga acatatccac agccttgcgc gcgtctatca gcgcgatgtc ctgcggcgtg
      541 gttacgatga cggacccggt tacggggatg cgttgggaca gcgtgagttg gatgtcgccc
      601 gtgccggggg gcaggtcgat gaaaagatag tccacttcgt cccactcgct ttggaacatc
      661 agctgctgca aggcttggct gaccatcggc ccgcgccaga cgacggcttg gccggtatcg
      721 actagaaagc cgatggacat aacctgtatg ccgtctgaag attcgacggg aatcagtttt
      781 tggtttttct gatcgggttt gcggtcgtgt acgcccagca tcgtgggctg gctcggaccg
      841 taaaggtcgg catcgagcac gccgacgcgt gcgcccatgc gcgccattgc ggcggcaagg
      901 ttggcagtgg tggttgattt gcccacgccg ccttttcccg atgcgacggc aatgatgttt
      961 ttcacgcctt tgatggtggc gacgccgggc cggactttgt gtgtgccgat ttcggtgtct
     1021 atggacagat ggatgtgcgc gccgcctgtt tcgggcatca gggtttcttg tacggcatcg
     1081 gcgagggcgg cggcgatgtg cgcgacaggg aagccgaaat gcagggcgat gtggatgccg
     1141 tctgaacgct gtccgaccga acggacggcc ttttcgccgc cgagcgtgcg tgccgtattc
     1201 ggaacggcga cggtgtcgag gagggtgcgg atgttttgta tattcatgat gtgtgcctct
     1261 gttcgcaagt cgtgtgaggg gaagatagac ggaaagtgta catgattttg ggcagtttgc
     1321 ttttttcttg tgcgaacttt tacacggtat gcggcggatt gtgaaaatcc gttgccaaaa
     1381 tatcccgtgc aaacagcagt ctgcaattaa gcctgtaaaa tttgcttgac ggcggggaac
     1441 ggggctgtat aatgcacggc ttatcgggtc gttagctcag ccggtagagc agcggacttt
     1501 taatccgttg gtcgagcgtt cgaatcgctc acgacccacc agataaccgg aagccaagtt
     1561 tcgagacttg gcttttttgt ttgcctgccg gggcgtaccg tttgcggggg tgtatgaaaa
     1621 agaaactgct ttcgggcatc aaatttgccg ttcggacggc attggtgttt ttgctggtgt
     1681 cgctgttttt ggattgggta cgcaagcccg acgaacctgc cggggcggca gggcggcctt
     1741 tgaccctgct gtcggggcag cggctgactt tgggacagtt tagccgggat aggacggtgc
     1801 tggtgtattt ttggggaagc tggtgcggcg tgtgccgtta tcagtcgccg ataatcgatg
     1861 atttggcggc ggacggcgtg ccggtcgtcg gcgtggcggt acgttccggc agcgcgtccg
     1921 aagtggcggc atatatggca aaacggggct tgggctttcc gactgtcagc gatgaggacg
     1981 ggggtttggc aaggtcttgg cggattgccg caacgcctgc cgtcgttttg gtcaaaaatg
     2041 ggaaaatggt ccgctatacg acgggaatca gcagttattg gggcttgcgc gcacgaattt
     2101 ttcaggcgga ttttttcggt taacttttgt ttttgtcaaa ctttccgcta tgtagagaat
     2161 caaacggcat atgccttgcg cggcggatgc ggcggctttt gttttttggg aaagagccgg
     2221 cgcgcctgcc gatgtaatcc cttgttgtga ttgtgggaaa aatagattaa aatataacta
     2281 ttaaaatatt ttcagatagg attatcggaa ttaaagtctt ttatacccgg tcgtccgatg
     2341 aggtttatag cgtattgttg ctatatgttc gttttgttat ataacggttg catcaaaaat
     2401 tacgcccaca ggctttcccg acggtttgaa agtttgattt tcgataactt ggagacttaa
     2461 acaatgccta cccaatcaaa acatgcgtct atcaatatcg gtctgataca ggcaagggaa
     2521 gccctgatga cccaattcag gcctattctg aatcaggcga atattaccga tcagcaatgg
     2581 cggattatcc gtcttttggc ggaaaacggc acattggatt ttcaggattt ggcgaatcag
     2641 gcgtgcattt tgcgccccag cctgaccggt atcctgaccc gccttgaaaa agcggggttg
     2701 gtcgtccgcc tgaaaccttc caacgaccaa cggcgcgttt atctgaagct gacttccgag
     2761 ggcgagaagc tgtatgagga aatcggcgaa gaagtggacg agcgttacga cgctatcgag
     2821 gaagtgctgg gccgcgagaa aatgctgctg cttaaagacc tgttggcaga acttgccaaa
     2881 atcgaggatg cgttgaactc gtaatacgcc gtaatgcgcg gaaacgtccg accgacggct
     2941 ttttgaatca gaactgctgc acatggggga tgccttgtgt gcagcattct tatatagggg
     3001 acggtttaaa ggggaaaaat ggcggatttg cagaaaactt ttcaaacttc gttccgtgat
     3061 gcgatggcat cctgtgcggc aggcgttcat gtcatcacga cagacggtgc ggcagggcgt
     3121 tacggcatta caatgacggc ggttgcgccg gttaccgacg agccgccgac cgtgatgctg
     3181 tgcataaacc ggagtgcgcg aatcattccg atcctgtcgg aaaacggcag cctctgcatc
     3241 aatatgctgg cggacgaaca tcaggatgtt gccgaacatt ttgccgggct gaccggcctg
     3301 tcgcccgaag agcggtttgc ctaccatatt tggcatcgcg gcaaaacggg acaacttgaa
     3361 atagaaggcg cgttggcgca cctgcacggg catattgtcg gcaaacatga aatcggcacg
     3421 cattttgtgt tttacgtcag gctcgacgaa atcaaaaact gcgggtgcaa acgccccgcg
     3481 ctgctgtatt tcagacggca gtttaggcct ttagactgat attcggacag atatatgaaa
     3541 gcgatgatac tggcggcagg acgcggcgag cgtatgcgcc ctttgaccga caccactccg
     3601 aagccgctgc tcgatgtggc gggtaagcct ctaatcggtt ggcacttgtg ccgtctgaag
     3661 caggcggggt ttaccgaaat cgtcatcaac cacgcttggc tgggtcggca gatagaaaat
     3721 gctttgggcg acggctcggc ttatggcgtg aacatcgcct attcgcccga acccgcaggc
     3781 ggtttggaaa cggcaggcgg catcgcgcag gcattgccgc tgttgggtgg gcagccgttt
     3841 ttggtggcca acggcgacgt gctgaccgac atcgatttta ccgccgcgtt tcagacggca
     3901 tcgtccctgc ccgggcacat ttccgcacat ttgtggctgg tgggaaatcc cccgcacaac
     3961 cccgacggcg atttttccct gctgcccgac ggcagcgtgc ggccggaagt atccggcggc
     4021 aacggactga cattcagcgg cgtgggtatt taccgtcctg aaatgtttga cggaatcgaa
     4081 gcgggcagtg tggcaaaact cgcgcccgta ttgctgaacg aaatgcggca aaaccgcgtg
     4141 agcggtcaga agcatacggg cctgtggctg gatgtcggca cggtatgccg tctgaaggaa
     4201 gcgcaagccc ttgcagcggc ttggaagtga aaactcggtt tcaggcggta tggcggattc
     4261 ggtttaacgt ttcagtgcca acgtcaacac gccgccgtaa ccagccccaa gtctatccat
     4321 tcctgcgtgt tcgggcgttc gtctaagaaa atcaccgcca tcagcgcgac caagaccagg
     4381 ctgaatttgt cgacgggcgc gacttgcggg gcgttgccca gttgcagggc tttgaagtag
     4441 gcgagccaag atgcgccggt ggcgagtccg gacaaaacca gaaacgtccg gttgcgcccc
     4501 gtgaagccgt ttacgccctg ccatttgccg gtgtaggtca aaaacaatac caaggcggcg
     4561 aggatgacca aggtgcggat aaaggcggcg aaatccgaat ctatgccctg caagcccatt
     4621 ttggcgaaaa cggcggtcaa tgaggcgaag cctgccgatg ccaatgccca aaacagccat
     4681 gcgttgctgc ccatgttttc tcctttgatt gtgaacaata tgaacggtat ttttgttgct
     4741 gcgtcaaaaa tttcactgcg ggtttggtgc ggataacgtt ataatatgcc tgatattatt
     4801 ttcaatccac ctgtttgtcg cctgatgctt tcagacggca tgtccctcct catttctaaa
     4861 ggaaaatcat gagcttcaaa accgatgccg aaaccgccca atcctccacc atgcgcccga
     4921 ttggcgaaat tgccgccaag ctgggtttga acgttgacaa cattgagcct tacggtcatt
     4981 acaaagccaa aatcaatcct gccgaagcgt tcaagctgcc gcaaaaacaa ggcaggctga
     5041 ttttggttac cgccatcaac ccgactccgg cgggcgaagg caaaaccacc gtaaccatcg
     5101 gtttggcgga cgcattgcgc catatcggca aagactctgt gattgctttg cgcgagcctt
     5161 ctttgggtcc ggtgttcggc gtgaaaggcg gcgcggcagg cggcggctac gcgcaagttt
     5221 tgccgatgga agacatcaac ctgcacttca ccggcgactt ccacgccatc ggtgcggcga
     5281 ataacctcct cgccgccatg ctcgacaacc atatctacca aggtaacgag ttgaacatcg
     5341 accccaaacg cgtgctgtgg cggcgcgtgg tcgatatgaa cgaccgccag ttgcgcaaca
     5401 tcatcgacgg tatgggcaag cctgttgacg gcgtgatgcg tcccgacggc ttcgacatca
     5461 ccgtcgcctc cgaagtgatg gcggtattct gccttgccaa agacatcagc gatttgaaag
     5521 agcgttttgg caatattctc gtcgcctacg ccaaagacgg cagccccgtt tacgccaaag
     5581 atttgaaggc acacggcgcg atggcggcat tgctaaaaga tgcgattaag cccaatttgg
     5641 tgcaaaccat cgaaggcact ccggtctttg tacacggcgg cccgttcgcc aacatcgccc
     5701 acggctgcaa ctccgttacc gcaacccgtc tggcgaaaca ccttgccgat tacgccgtaa
     5761 ccgaagcagg cttcggcgcg gacttgggtg cggaaaaatt ctgcgacatc aaatgccgcc
     5821 ttgccggttt gaaacctgat gcggcagtcg tcgtggcgac tgtccgcgcc ctgaaataca
     5881 acggcggcgt ggaacgcgcc aaccttggtg aagaaaacct cgaagccttg gcaaaaggtt
     5941 tgcccaacct gttgaaacac atttccaacc tgaaaaacgt attcggactg cccgtcgtcg
     6001 ttgcgctcaa ccgcttcgtg tccgactccg atgccgagtt ggcgatgatt gaaaaagcct
     6061 gtgccgaaca cggcgttgaa gtttccctga ccgaagtgtg gggcaaaggc ggcgcgggcg
     6121 gcgcggattt ggcgcgcaaa gtcgtcaatg ccatcgacaa ccaacctaat aacttcggtt
     6181 tcgcctacga tgtcgagttg ggcatcaaag acaaaatccg tgcgattgcc caaaaagtgt
     6241 acggcgcgga agatgtcgat ttcagcgcgg aagcgtctgc cgaaatcgcc tcgctggaaa
     6301 aactgggctt ggacaaaatg ccgatctgca tggcgaaaac ccaatattca ttgagcgaca
     6361 acgccaaact cttgggctgc cccgaaggct tccgcatcac cgtacgcggt atcactgttt
     6421 ccgccggcgc gggcttcatc gttgcgttgt gcggcaatat gatgaaaatg ccgggcctgc
     6481 cgaaagttcc ggctgccgag aaaatcgatg tggacgaaca cggcgtgatt cacggcttgt
     6541 tctgaacggt ttttgaaacc ggatgccgtc tgaagccgtt tcagacggca ttttttcgga
     6601 acgcgggcgg cggtatgcta taatccgccg ttaaatttct ctattttcag gaaaaaccat
     6661 gagtttgaaa tgcggcatcg tcggtttgcc caacgtcggc aaatccaccc tttttaacgc
     6721 gctgacccaa tcgggcatcg aagcggcaaa ctatcccttc tgcaccatcg aacccaacgt
     6781 cggcatcgtc gaagtgcccg acccgcgtat ggcggagctg gcgaaaatcg tcaatccgca
     6841 aaaaatgcag cccgccatcg tcgagtttgt cgatattgcc ggcttggttg cgggcgcgag
     6901 caaaggcgaa ggcttgggca accggttcct tgccaacatc cgtgaaaccg atgccatcgt
     6961 caacgtcgtg cgctgctttg acgacgacaa catcgtccac gtttccggca aagtcgatcc
     7021 gattgccgac atcgaaacca tcggcaccga attggcgctt gccgacttgg caagtgtgga
     7081 aaaagccatc gtccgcgaag aaaaacgcgc ccgatcaggc gacaaagacg cgcaaaaact
     7141 agtcgatttg tgcaaaaaac tgctgccgca tctggacgaa ggcaaacccg tgcgttcctt
     7201 cggtttggac gcggaagaac gcgcgctgct gaagccgctg ttcctgctga ccgccaaacc
     7261 tgcgatgtat gtgggaaacg ttgccgaaga cggttttgaa aacaacccgc acctcgaccg
     7321 cctgaaagaa ttggcggcaa aagaaaacgc ccccgtcgtt gccgtctgcg ccgcgatgga
     7381 gagcgaaatc gccgaattgg aagacggtga aaaagccgaa tttctcgccg aaatgggctt
     7441 ggaagaaccg ggcttgaacc gcctcatccg cgcgggttac gaccttttgg gactgcaaac
     7501 ctacttcacc gccggcgtga aagaagtccg cgcgtggacg atacacaaag gcgacaccgc
     7561 gccgcaagcc gccggcgtga tccatacgga ttttgaacgc ggcttcatcc gcgcccaagt
     7621 gattgcctac gatgattttg tctcgctcgg cggcgaagcc aaagccaaag aagccggcaa
     7681 aatgcgcgtg gaaggcaagg aatacgtcgt gcaggacggc gacgtgatgc actttttgtt
     7741 taacgtgtaa cccaaatgcg gcaggttttc agacggcttt gccggaaatg ccgtctgaag
     7801 ccggttttgg tggttttcga cgttcccata ccgccggaat gcagccgcat caaaataaaa
     7861 tcccgcccgc atttccgatt tgccctcccc gattcctgca aaacaaaccg cctgccctgc
     7921 cgttacggga agccgtccgg tattccgaat atcccgaacc ccgatacaaa atgacctttc
     7981 agacggcatt tgcacagccc gccgcgtttc aagtaaaaac attatgagcc aagccttacc
     8041 ctaccgcccg gacatcgaca cattgcgcgc cgccgccgtc ttgtccgtca tcgtgttcca
     8101 tatcgaaaag gattggctgc cgggcgggtt tctcggtgtc gatatattct ttgtgatttc
     8161 aggctttttg atgacggcga tcctccttcg cgaaatgtcc ggggggcgtt tcttcctcaa
     8221 gacattttat atccgccgca tcaaacggat tttgcccgca tttttcgccg tattggcggc
     8281 aacgctggca ggcggcttct ttttattcac caaagatgat ttctttcttt tgtggaaatc
     8341 cgcgctgacc gccttgggtt tcgcctccaa cctgtatttt gcaaggggga aggattattt
     8401 cgatcccgcg caggaagaaa agcccctgct gcacatctgg tctttgtcgg tcgaagaaca
     8461 attttacttt gtctttccga tattgctgtt gcttgtcgcc cgcaaaagcc tgcgcgtaca
     8521 gttcggcttc ctcgccgcat tgtgcgcctt aagccttgcc gcttccttta tgccttccgc
     8581 gctcgataaa tattacctgc cccacctgcg cgcctgcgaa atgctggtcg gatcgctgac
     8641 cgccgtgcgg atgcggtacc ggcaacagcg gaatcccgcc gtcgggaaac ggtatgccgc
     8701 cgtcggcgca ttgttttccg cgtgcatact gtccgcctgc ctgtttgcct attcggaaca
     8761 aaccgcctat ttcccgggct ccgccgccct gattccctgt ctggctgttg ccgcgctgat
     8821 ttatttcaac cattacgaac acccgcttaa aaaatttttc caatggaaaa tcaccgttgc
     8881 cgccggtttg atttcctatt cgctttatct gtggcattgg ccgatattgg cctttatgcg
     8941 ctatatcggc ccggacaacc tgccgcctta ttcgccggcg gcagcgatcg tcctgaccct
     9001 ggcgttttcc ctgatttctt atcactgcat cgaaaagccg tttaaaaaat ggaaaggctc
     9061 gttcgcacaa tccgttttat ggatttatgc cttgcctatg ctcgttttgg gcgcgggctc
     9121 gtttttcgcg atgaggctgc cgtttatggc gcaatacgac cgcttggggc tgacgcgttc
     9181 caacacctcc tgccacaaca ataccggcaa acaatgcctg tggggggata cggaaaaaca
     9241 gccggaactg ctggttttgg gcgactccca cgccgaccat tacaaaacat tcttcgatgc
     9301 cgtgggcaaa aaagaaaaat ggtccgccac tatggtttcc gccgacgcct gcgcctatgt
     9361 ggaaggctac gcgtcccgtg tgttccaaaa ctgggccgcc tgccgcgccg tttaccgcta
     9421 tgccgaagaa cacctgcccc ggtatccgaa agtggttttg gcgatgcgct ggggcagcca
     9481 gatgcccgaa aacagccgct cccttgccta cgatgccggt tttttccaaa aattcgaccg
     9541 tatgctgcac aaactctcat ccgaaaaaca agccgtttac ctgatggcgg acaacttggc
     9601 ttcgtcttac aacgtccagc gcgcctatat cttgtcttca cgcataccgg gttgccgcca
     9661 aacactgcgc ccggacgacg aaagcaccct gaaagccaat gcccgcatca gggaattggc
     9721 agccaaatac cccaacgtct atattattga tgccgccgcc tatatccccg cagatttcca
     9781 aatcggcgga ttgccggttt actcggacaa agaccacatc aacccttacg gcggcacaga
     9841 attggcgaag cgtttttccg aaaaacaaag gtttctcgat acgcgccata accattgatt
     9901 cgcttaaatt tgttacaatc ggcggtttgc aaaaccgcta atttttttga aagagaccga
     9961 tgagcgtcat ccaagacctg caatcgcgcg gccttatcgc gcaaaccacc gacatcgaag
    10021 ccttagacgc tttgctgaac gaacaaaaaa tcgcccttta ctgcggtttc gacccgacag
    10081 ccgacagcct gcacatcgga cacctgctgc ccgtattggc attgcgccgc ttccaacagg
    10141 cggggcatac gccgattgca ctggtgggcg gcgcgaccgg tatgatcggc gaccccagct
    10201 tcaaagccgc cgaacgcagc ttgaattccg ccgaaaccgt tgccggctgg gtaggaagca
    10261 tacgcagcca attaacccct ttcttgagct ttgaaggcgg aaacgccgcc attatggcga
    10321 acaatgccga ctggttcggc agcatgaact gcctcgactt cctgcgcgac atcggcaagc
    10381 atttctccgt caacgccatg ctgaacaaag aatccgtcaa acagcgcatc gaccgcgacg
    10441 gcgcaggcat ttccttcacc gagttcgcct attccctgct gcaaggctac gacttcgccg
    10501 agttgaacaa acgccacggc gcggttttgg aaatcggcgg ttccgaccag tggggcaaca
    10561 tcaccgccgg tatcgacctg acccgccgcc tgaaccaaaa acaagtgttc ggtctgaccc
    10621 tgcctttggt taccaaatcc gacggtacca aattcggcaa aaccgaaggc ggcgcggtgt
    10681 ggctgaacgc gaaaaaaacc tcgccgtacc agttctacca gttctggctg aaagtcgccg
    10741 atgccgatgt gtataaattc ctgaaatact ttaccttcct gtccatcgaa gaaatcggtg
    10801 tcgtcgaagc caaagacaag gcaagcggca gcaagcccga agcgcaacgc atcctcgccg
    10861 aagaaatgac ccgcctgatt cacggcgaag aagcccttgc cgccgcgcaa cgcatttccg
    10921 aaagcctgtt tgccgaagac caaagccggc ttaccgaaag cgacttcgag cagctcgccc
    10981 tcgacggcct gcctgcattt gaagtttcag acggcatcaa cgccgtcgaa gccttggtca
    11041 aaaccggctt ggcagcgtcc aacaaagaag cgcgcggctt tgtcaatgcc aaagcggttc
    11101 tgctcaacgg caaaccggct gaagccaaca accccaacca cgccgccgaa cgccccgacg
    11161 atgcctatct gttgataggc gaatacaaac gtttcggcaa atacaccatc ctccggcgcg
    11221 gcaaacgcaa ccacgcgctt ttggtttgga aataatccga ttgccgcaga aatgccgtct
    11281 gaagctttca gacggcattt ttatcaaatg caaaacaccc tgcgcctgcc gatatgtcgt
    11341 catttccatg caggcgggaa ttcaaacttg tccgcacgga aacttatcgg gcaaaacggt
    11401 ttcttcagtt ctacgttcta gatgcccgct ttcg
//
LOCUS       DBJGGI010000065        11411 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.065, whole genome shotgun sequence.
ACCESSION   DBJGGI010000065 DBJGGI010000000
VERSION     DBJGGI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11411
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.065"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            76..1614
                     /gene="murJ"
                     /locus_tag="ACSIGJ_001711"
     CDS             76..1614
                     /gene="murJ"
                     /locus_tag="ACSIGJ_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HHQ1330530.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLIVVTALG
                     ILAAPWVIYVSAPGFTKDADKFQLSISLLRITFPYILLISLSSFVGSILNSYHKFGIP
                     AFTPTFLNISFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAAGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLASGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHAGLSLAIGLGACINAGLLFFLLRKHGIYRPGRGWAAFLAKMLL
                     ALAVMCGGLWAAQACLPFEWAHAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            1747..2703
                     /locus_tag="ACSIGJ_001712"
     CDS             1747..2703
                     /locus_tag="ACSIGJ_001712"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008126 - acetylesterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HHQ1330531.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQHPAPAYRREMLPDSTGKT
                     KTAYDFSGGISPDAPLVVLFHGLEGSSRSHYAAELMLAVRNRGWHGAVVHFRSCGGVA
                     NTAPVFYHLGDTAEIAFALDTLTARYREIYAVGVSLGGNAPAKYLGEQGKKALPHASA
                     AVSAPVDAEAAGSRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPRADEASEAV
                     TLFQPAHGGHAGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRH"
     gene            2754..3536
                     /gene="trpC"
                     /locus_tag="ACSIGJ_001713"
     CDS             2754..3536
                     /gene="trpC"
                     /locus_tag="ACSIGJ_001713"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HHQ1330532.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVLLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQGQLERFEALA
                     HELGMTVLLELHDETELEKCRNLTTPLRGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRARGVHTFLIGETFMRADDIGAEVGKLF"
     gene            3599..5902
                     /gene="recQ"
                     /locus_tag="ACSIGJ_001714"
     CDS             3599..5902
                     /gene="recQ"
                     /locus_tag="ACSIGJ_001714"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HHQ1330533.1"
                     /translation="MTHRPTARQILHEVFGYPEFRGRQEDVINTLAGGGSLTVLMPTG
                     GGKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIA
                     DKLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSRWGHDFRPEYQQLG
                     MLAERYPNIPRIALTATADAATRADIKHYLHLDDASEFVSSFDRTNIYYQVIEKNNGK
                     KQLLDFIRKEMTGQSGIVYCLSRKKVEDAAQFLRENGLNAIPYHAGLSMDVREENQRR
                     FTHEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAVSWLC
                     YGLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEASEPC
                     GHCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQL
                     STFGIGAELSDKEWRSVIRQCISLGYLTVNIARYQALQLTEAAKKILKGETEVMLRPL
                     KRDKPATRTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKM
                     PQNLNGLHDIYGLGEAKTERFGHGILKVCQNAADFSHDAVIRPQTEREQQLRQKLEAW
                     RYEQARAENCALHTVLSDESLADMLAATPETETDLEAVHGLGSVRAAKYGRDILAVCR
                     PFSDGIDETAKHKRCLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAEL
                     AAVYGVGEEKAARYGAAVLAVLERDAV"
     gene            complement(5939..7312)
                     /locus_tag="ACSIGJ_001715"
     CDS             complement(5939..7312)
                     /locus_tag="ACSIGJ_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HHQ1330534.1"
                     /translation="MDWRGNKPLGAAELADLKPLYKDFMYWERGLHMYKASAVVPTGY
                     VRVGNTAPLCGEDTQRYASFWGDGYDVYRQLRWRQIPEKQRKAFKKAAKSKNTVMFAG
                     REYGISKQNLSDVWDDFEDAMELKAFPCLSSLFLTKWHKNLYEYLEEYPFITRLCLEN
                     HGQTVLDFSNTRITDLSVDMTGVESLYLNEGLDSLNLKGEIKENCKVCTAGKGAGLIL
                     EVGKSVPKVRGLENLTAVNVMGIADFDMQNLSETYPKLKTIRLWGKPGNIANFSAVSG
                     FEDLEVFTAVDLFGFGADDIPHPDRLPKLHRLWMSSLPEEAAKAVKKLYKKRKEDGLD
                     LWIEKARKPEWLAQNFDNPFRDWDGAEHIPKSHAKKAAELYRKTRAGVVKLLGNPPEN
                     TGEGLAEAVKAYTGGFNKMDKKHFIDTVEREDIAEALETILDLIPDGSCADKEKLFEI
                     FDKNRNF"
     gene            7431..7541
                     /locus_tag="ACSIGJ_001716"
                     /pseudo
     CDS             7431..7541
                     /locus_tag="ACSIGJ_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7622..8410)
                     /locus_tag="ACSIGJ_001717"
     CDS             complement(7622..8410)
                     /locus_tag="ACSIGJ_001717"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHQ1330535.1"
                     /translation="MNPQDKCWQVHRHLAEHTDQRLTLVRNAPGHILLAGADADISRS
                     LLAKRYPPAVFEEYDSRADFLAAAAAARKGGFWQKLTGRGVVQHCQSPTVPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTLAELKSRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAVAWDDENAA
                     RSCVGAIFEREGGLGITLETVYGHAVKKLVLPQGENVVRFFPKR"
     gene            complement(8407..9156)
                     /gene="bioH"
                     /locus_tag="ACSIGJ_001718"
     CDS             complement(8407..9156)
                     /gene="bioH"
                     /locus_tag="ACSIGJ_001718"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HHQ1330536.1"
                     /translation="MPDAAKKVYLIHGWGANRHAFDDLMPRLPATWPVSAVDLPGHGD
                     APFAQPFDIEAAADGIAAQIDTSADILGWSLGGLVALYLAARHPDKVRSLCLTASFAR
                     LTAAEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTPDAAEIIGRILPDLAR
                     CGTPQALQEALDAAERADVRHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRL
                     VVMEKAAHAPFLSHAEVFAALYRDFVEGGLT"
     gene            9155..9880
                     /locus_tag="ACSIGJ_001719"
     CDS             9155..9880
                     /locus_tag="ACSIGJ_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HHQ1330537.1"
                     /translation="MNFLSCWRRIADAPTIRRCVLCHGSSGVSDGICAGCNTDLASFR
                     TDAANSCPLCFRHVQGGAVCGGCQKKPPAFDRMWASLHYEPPVSNMIRALKHLADLGM
                     AQPLADLTMQNPPDRLSDECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQIL
                     PRHTVFRHHRPPQSTLKGGERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELA
                     KTLKKSGANRICCWTLARTPMKK"
     gene            9945..10409
                     /gene="trmL"
                     /locus_tag="ACSIGJ_001720"
     CDS             9945..10409
                     /gene="trmL"
                     /locus_tag="ACSIGJ_001720"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HHQ1330538.1"
                     /translation="MLTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKSLAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMRPGSRSMNLSNTVSVILFEAWRQHGYAGGV"
     gene            10537..11223
                     /locus_tag="ACSIGJ_001721"
     CDS             10537..11223
                     /locus_tag="ACSIGJ_001721"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HHQ1330539.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AEFTQTGNASWYGGRFHGRKTSGGDRYDMNAFTAAHKTLPIPSHVRVTNTKNGKSVIV
                     RVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGQSAPVAENKDIFIDLK
                     SFGTEHEAQAYLNQAAQNFAASSSSPNLSVEKRRYEYVVKMGPFASQERAAEAEAQAR
                     GMVRAVLTSG"
     gene            11284..>11411
                     /locus_tag="ACSIGJ_001722"
     CDS             11284..>11411
                     /locus_tag="ACSIGJ_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HHQ1330540.1"
                     /translation="MNIKHLLLTAAATALLGISAPALAHHDGHGDDDHGHAAHQHG"
BASE COUNT         2495 a         3498 c         2996 g         2422 t
ORIGIN      
        1 tgatttttgt taatccacta taaagtctga tcctgccgcc ctaaagggcg gggtttcagc
       61 cgaaaaggaa acacgatgaa tatgcttgga gctttggcaa aagtcggcag cctgacgatg
      121 gtgtcgcgcg ttttgggatt tgtgcgcgat acggtcattg cgcgggcatt cggcgcgggt
      181 atggcgacgg atgcgttttt tgtcgcgttc aaactgccca acctgcttcg ccgcgtgttt
      241 gcggaggggg cgtttgccca agcgtttgtg ccgattttgg cggaatataa ggaaacgcgt
      301 tctaaagagg cgacggaggc ttttatccgc cacgttgcgg gaatgctgtc gtttgtgctg
      361 atcgtcgtta ccgcgctggg catacttgcc gcgccttggg tgatttatgt ttccgcgccc
      421 ggctttacca aagacgcgga caagttccaa ctttccatca gcctgctgcg gattacgttt
      481 ccttatatat tattgatttc tttgtcttct tttgtcggat cgatactcaa ttcctaccat
      541 aagttcggca ttcccgcgtt tacgcccacg tttttaaaca tctcttttat cgtattcgca
      601 ctgtttttcg tgccgtattt cgatccgccc gttaccgcgc tggcgtgggc ggtttttgtc
      661 ggcggtattt tgcagctcgg tttccaactg ccgtggctgg cgaaactggg ctttttgaaa
      721 ctgcccaaac tgaatttcaa agatgcggcg gtcaaccgcg tcatgaaaca gatggcgcct
      781 gcgattttgg gcgtgagcgt ggcgcaaatt tctttggtta tcaacacgat tttcgcgtct
      841 tatctgcaat cgggcagcgt ttcatggatg tattacgccg accgcatgat ggagctgccc
      901 ggcggcgtgc tgggggctgc actcggtaca attttgctgc cgactttgtc caaacactcg
      961 gcaaaccaag atacggaaca gttttccgcc ctgctcgact ggggtttgcg cctgtgcatg
     1021 ctgctgacgc tgccggcggc ggccggactg gcggtattgt cgttcccgct ggtggcgacg
     1081 ctgtttatgt accgagaatt cacgctgttt gacgcacaaa tgacgcaaca cgcgctgatt
     1141 gcctattctt tcggtttaat cggtttaatt atgattaaag tgttggcatc cggcttttat
     1201 gcgcggcaaa acatcaaaac gcccgtcaaa atcgccatct tcacgctcat ctgcacgcag
     1261 ttgatgaacc tcgcctttat cggtccgttg aaacacgccg ggctttcgct cgccatcggc
     1321 ctgggcgcgt gcatcaacgc cggattgttg ttcttcctgt tgcgcaaaca cggtatttac
     1381 cggcccggca ggggttgggc ggcgttcttg gcgaaaatgc tgctcgcgct cgccgtgatg
     1441 tgcggcggac tgtgggcggc gcaggcttgc ctgccgttcg aatgggcgca cgccggcgga
     1501 atgcggaaag cggggcagct ctgcatcctg attgccgtcg gcggcggact gtatttcgca
     1561 tctctggcgg ctttgggctt ccgtccgcgc catttcaaac gcgtggaaag ctgaccgatg
     1621 ccgatatcct ttttcggaaa acgctttttc cgcgcctata tccgggcgcg catcgcccgc
     1681 gtccgcaccg gccggacggg ctgccgcatc accaatgccg tctgaaaaca gaaaaaccga
     1741 taccttatga ttttgacacc gccggacacg ccctttttcc tccgcaacgg caatgccgac
     1801 acgattgccg ccaaattcct gcaacacccc gcacccgcat accgccgcga gatgcttccc
     1861 gacagcacgg gtaaaaccaa aaccgcctac gacttttcag gcggcatttc gcccgatgcg
     1921 ccgctggtcg tgctgtttca cggtttggaa ggaagcagcc gcagccatta cgcggccgaa
     1981 ctgatgctcg cggtacgcaa tcggggttgg cacggcgcag tcgtccattt ccgcagctgc
     2041 ggcggcgtag cgaacaccgc cccggtgttc taccacttgg gtgataccgc cgaaatcgcc
     2101 tttgctttgg acacgctcac cgcgcgttac cgtgaaatat acgccgtcgg cgtatcgctg
     2161 ggcggcaacg cgccggcaaa atatttgggc gaacagggca aaaaggcatt gccgcacgcc
     2221 tcggccgccg tatccgcccc cgttgatgca gaggcggcag gcagccgctt cgacagcggc
     2281 atcacgcggc tgctctacac gcgctacttc ctccgcacac tgatacccaa agcacgttcg
     2341 ctccaaggtt ttcagacggc atttgccgca gggtgcaaaa cactgggcga gtttgacgac
     2401 cgtttcaccg caccgctgca cggctttgcc gaccggcacg actactaccg ccaaacttcc
     2461 tgcaaaccgc tgctcaaaca cgttgccaaa ccgctgctcc tgctcaatgc cgccaacgac
     2521 cccttcctgc cgcccgaagc cctgccccgt gcggacgaag cgtccgaagc cgttaccctg
     2581 ttccaacctg cacacggcgg gcacgccggc tttgtcagca gcaccggcgg caggctgcac
     2641 ctgcaatggc tgccgcagac cgtcctgtcc tattttgaca gcttccgcac aaacaggcat
     2701 taacggtttg atgctaatat tccccctttc cccggacaaa tacggaacac gacatgaccg
     2761 acatcctcaa taaaatcctt gccaccaagg cacaggaagt tgccgcccaa aaagccgccg
     2821 tcaacgccga acacatccgc gcacttgccg cagaagccgc gcccgtccgc agcttcatcg
     2881 attcgatacg cggcaaacac cgcctaaacc tgcccgccgt cattgccgaa atcaaaaagg
     2941 caagcccgag caaagggtta atccgccccg acttccgccc tgccgagatt gcccgcgcct
     3001 atgaaaacgc cggggcggcg tgtttgtcgg tactgaccga cgaaccctat ttccaaggtt
     3061 cgcccgaata cctcaaacag gcgcgcgaag ccgtattgct gcccgtgctg cgcaaagact
     3121 tcatcatcga cgaataccag gtttatcagg cgcgcgcatg gggggcggat gccgtcctgc
     3181 tgattgccgc cgcactggaa cagggacaat tggaacgctt tgaggcgctg gcgcacgaat
     3241 tgggcatgac cgtcctgctc gagctgcacg acgaaaccga attggaaaaa tgccgcaacc
     3301 tgaccacgcc gttgcggggc gtaaacaacc gcaacctgcg gacttttgaa gtctccctcg
     3361 accaaaccct gtcgctgctg cccgcgctgg aaggcaaaac cgtcgttacc gaaagcggca
     3421 ttacaggcaa ggcggatgtg gaatttatgc gggcgcgcgg cgtgcatacc ttcctgatcg
     3481 gcgaaacgtt tatgcgtgcc gacgatattg gggcggaagt gggcaaactc ttctaaaatc
     3541 ccgatttcag acggcatatt gccgccgacc gaccaacacc cttaccggac acggaacaat
     3601 gacgcaccga cccaccgcca gacagatttt gcacgaagta ttcggctatc ccgaatttcg
     3661 cggcaggcag gaggatgtca tcaatacttt ggcaggcggc ggcagtttga cggtgctgat
     3721 gccgacgggc gggggcaagt ctttgtgtta ccagattccg gcgctgatgc gcgaaggcgt
     3781 ggcggttgtc gtatcgccgc tgattgcgct gatgaacgac caagtggcca gcctgcatgt
     3841 ggccgggatt gaagcggcgg cagtcaacag cggcacatcg gcagatgagg cgcgcgagat
     3901 tgccgacaag cttgcccaag gccgtctgaa gctgctttat gtcgcgccgg aacgcttggt
     3961 taccgaccgc tttttacgtt ttctcgacca acaaaccgtc agcctgttcg ccattgacga
     4021 agcgcactgc gtcagccggt ggggacacga tttccgcccc gaatatcaac agctcggtat
     4081 gcttgccgaa cgctatccga acatcccgcg catcgccctg accgccaccg ccgatgccgc
     4141 cacgcgcgcc gacatcaagc attatctgca cttggacgac gcgtccgaat ttgtctccag
     4201 ctttgaccgc acgaatattt attatcaggt tatcgaaaaa aacaacggca aaaaacaatt
     4261 gctggatttc atccgcaaag aaatgacggg gcaaagcggc attgtgtatt gcctaagccg
     4321 caaaaaggtt gaagatgcgg cgcagttttt gcgtgaaaac ggattaaacg cgattccgta
     4381 tcatgccggt ttgagcatgg acgtgcgcga ggaaaaccaa cgccgcttta cgcatgaaga
     4441 caatattatc gtggtggcga ccgtggcgtt cggcatgggc atagacaaac ccgacgtgcg
     4501 ctttgtcgcc catctcgata tgccccagag tgtcgaacat ttctatcagg aatcagggcg
     4561 cgccggccgg gacgggctgc ctgccgtgag ttggctgtgt tacggcttga acgattgggt
     4621 gttgctgcgc gaacggattg ccgaaggcaa cagcgacgag gtgcaaaagc aaatcgaaat
     4681 gcaaaaactc gatgccatgc tttccgtctg cgaaaccgcc gcctgccgcc gcgtactgct
     4741 gctcaaacat ttcggcgaag catccgaacc ctgcggacat tgtgacaact gcctgcatcc
     4801 gcccgtacgg tttgacggca cggtgttggt gcaaaaatta ctcagctgcg tgtaccgcgc
     4861 cggacaacgc tttgccgccg gttacatcac caaccttttg cgcggcaaaa gcgacgattg
     4921 gatacgcggc aaccggcacg agcaactgtc cacattcggc atcggcgcgg aattgtccga
     4981 caaagaatgg cgcagcgtca tccgccagtg catcagcctc ggctacctca ccgtcaacat
     5041 tgcccgatat caggcattgc aactgaccga agccgccaaa aaaatcctca aaggcgaaac
     5101 cgaagtgatg ctgcgcccgc tcaagcgcga caagcccgcc acccgcaccc tcaaagacaa
     5161 ctggctgcgt accgaacgcg aagaacgcct gtggcaggca ttgcgcgttt ggcgcatgaa
     5221 acaggctgaa gccgaaggca tccccgccta tatgattttc ggcgacaaaa ccctgcgcga
     5281 ccttgtcgaa aaaatgccgc aaaacctcaa cgggctgcac gacatctacg gcttgggcga
     5341 agccaaaacc gaacgtttcg gacacggcat actcaaagtc tgccaaaacg ctgccgactt
     5401 tagccacgat gccgtcatcc gtccgcaaac cgaacgcgaa caacaactgc gtcaaaaact
     5461 cgaagcctgg cggtatgaac aggcaagggc ggaaaactgc gccctgcata ccgtcctctc
     5521 cgacgaaagc cttgccgata tgcttgccgc cacgcccgaa accgaaaccg acctcgaagc
     5581 cgtacacggc ttgggcagcg tacgcgccgc caaatacgga cgggacatcc tcgccgtctg
     5641 ccgtccgttt tcagacggca tcgatgaaac cgccaaacac aaacgctgcc tgatgcgcgc
     5701 cctgattcaa tggtgcaacg aaaccgcaaa acacgaacag tccgaaccct accgcattct
     5761 cagcaaagcc gccctgcgcg ccattgccgc caaacagccg gaaggtttgg cggagcttgc
     5821 cgccgtatac ggcgtaggcg aagaaaaagc cgcacgttac ggtgcggcgg tgttggcggt
     5881 gttggaacgg gatgccgtct gaagcccgtt accaagtttc agacggcatt gcctctgttt
     5941 aaaaattcct gtttttatca aatatttcaa acagtttttc cttgtcggca caagacccat
     6001 ccggtatcag gtccaatatc gtttccaacg cctcggcaat atcttcccgc tctaccgtgt
     6061 cgataaagtg ttttttgtcc attttgttga aacctccggt ataggctttg accgcctctg
     6121 ccagcccctc ccctgtgttt tcaggcggat tgccgagcag tttgaccacg ccggcacggg
     6181 ttttccggta cagctccgcc gcttttttgg catggctttt cgggatatgc tccgcgccgt
     6241 cccaatcgcg gaaggggttg tcgaaatttt gcgccagcca ttcgggtttt cgggcttttt
     6301 ctatccacag gtccagcccg tcttccttcc gtttcttata aagtttcttc actgccttcg
     6361 ccgcctcttc gggcaggctg ctcatccaca gcctgtgcag cttcggcagc cggtcgggat
     6421 gagggatgtc gtccgcgccg aaaccgaaca aatctacggc agtgaacact tccaaatcct
     6481 caaatcccga tacggcggaa aaattggcga tattgcccgg tttgccccac agccgtatgg
     6541 ttttgagctt gggatacgtt tccgacaggt tttgcatatc gaaatcggca atgcccatga
     6601 cgttgacggc ggtcaggttt tccaaacccc gcactttggg aacgcttttg cccacttcca
     6661 aaatcagacc cgcgcccttt ccggcggtac agaccttgca gttttccttg atttcgcctt
     6721 tcaggttcag gctgtccaag ccttcgttca gataaagcga ttccacgccg gtcatatcga
     6781 cagaaaggtc ggttatgcgc gtattgctga aatccagcac ggtctgaccg tggttttcca
     6841 gacacaatcg ggtgataaac ggatattcct ccagatattc atatagattt ttatgccact
     6901 tggtcagaaa cagcgaagac aggcagggaa acgccttcag ttccatcgcg tcttcaaaat
     6961 catcccaaac atcgctcaaa ttctgtttgg atatgccgta ttcccgtccg gcaaacatca
     7021 cggtattttt gcttttggcg gcttttttga atgcctttct ctgtttttcg ggtatctgcc
     7081 gccatctcaa ctgacggtac acgtcgtagc cgtcgcccca aaaagaggca taccgctgcg
     7141 tgtcttcgcc gcacagcggc gcggtattcc caacccgtac atatcccgtc ggcacgacgg
     7201 cacttgcctt atacatgtgc agaccgcgtt cccaatacat aaagtctttg taaagcggtt
     7261 tcaaatccgc cagctccgcc gccccaagcg gtttgttgcc ccgccagtcc aaagccagca
     7321 gggtcgcatg tgtttttttg ccaccgtgtt cttcaactcg ggtaacctgg caggcagtgt
     7381 actgcttcag tctgatgtta tagacgcaat atacgtctcc ggcatcggca catagtggat
     7441 taaatttaaa tcaggacaag gcggcgaagc cgcggacagt acagatagta cggcaaggcg
     7501 agccaacgcc gtaccggttt aaatttaatt cactatacat cggcatttcc tttatttctg
     7561 tcggttttca cagacaaatg ccgtctgaaa cttggaaacg gcttcagacg tcatctgcac
     7621 atcatctctt cggaaaaaaa cgcaccacgt tctccccctg cggcagcacc agttttttca
     7681 cggcgtgtcc gtacaccgtt tccagcgtaa tgcccaaacc gccttcccgt tcaaatatcg
     7741 ccccgacaca cgaacgcgcg gcgttttcgt cgtcccacgc caccgcccgc caaacgccca
     7801 gcgtgtccat atccgcccaa aacgtttccg cctttttgta gtccaacacc agcttcgccg
     7861 tatcggtaac tggatcgtaa aagccgtttt cggcgagcat atcgcccaag tcgtgcatat
     7921 cgggaaacat cacgctgcgg ctttcaatgc cgttttcttt cagacgggat ttcagttccg
     7981 ccaaggtatc gcgcccgaag caggtgaaaa acagcagccc gtccgtcttc agcgcgcgcg
     8041 cccagttgtg cagcacggga aggatttgtt ccgccgccaa cagtccgagg ttcgaccaca
     8101 acatatcggc acacgcttcg ggcagcggca cggtcgggga ttggcagtgc tgcaccacgc
     8161 ccctgcccgt cagtttttgc cagaaaccgc ccttgcgcgc ggctgcggca gccgccaaaa
     8221 aatccgcacg ggaatcgtat tcttcaaata ccgccggcgg atagcgcttc gccaacaggc
     8281 tgcggctgat gtccgcatcc gcaccggcaa gcaggatatg cccgggcgcg ttgcggacga
     8341 gtgtcaggcg ttggtcggta tgttcggcaa gatggcggtg aacctgccag catttgtcct
     8401 gcggattcat gtcaaacccc cttcgacaaa gtcgcggtac agcgcggcaa acacttccgc
     8461 atggctcaaa aacggcgcgt gtgccgcctt ttccatcaca accagcctgc ttcctttcaa
     8521 atgccgatgc agatactcgc ccatacgcgg cggtgtaatc gcgtctttgc cgccgaacac
     8581 cagcagtacc ggaacatcta tcttgtccaa caaatgccgc acatccgccc tttccgccgc
     8641 gtccaaagct tcctgtaagg cttgaggcgt gccgcagcgc gccaaatcgg gcaggattct
     8701 gccgatgatt tcggcggcat caggcgtgtg cagaagctgg agttgtagaa actgtttgat
     8761 atgtttggca taatccgttc ggaacgcgcc gaccattttg cccagtgcag gcgcggcaag
     8821 cccttcggga tagtcttcgg cagccgtcag ccgtgcgaaa ctcgccgtca ggcagagcga
     8881 acggactttg tcgggatggc gcgccgccag atacagcgcg accaatccgc cgagtgacca
     8941 gccgagaatg tcggccgacg tatcgatttg agcggcaatg ccgtcggcag ctgcctcaat
     9001 atcgaagggt tgcgcgaagg gcgcgtcccc gtgtccgggc aaatcgacgg cggacaccgg
     9061 ccacgttgcg ggcaggcgcg gcatcaaatc gtcgaacgcg tggcggttcg ccccccaacc
     9121 gtgtatcagg taaacttttt tggcagcatc aggcatgaat tttctctctt gttggcggcg
     9181 catcgcagac gcgcccacta tcaggcgttg cgtattatgc cacggttcgt ccggcgtttc
     9241 agacggcatc tgcgccggct gcaataccga tttggcaagt ttccgcaccg acgcggcaaa
     9301 cagctgtccc ctgtgtttca ggcacgttca aggcggcgcg gtgtgcggcg gatgtcagaa
     9361 aaaaccgccc gcattcgacc ggatgtgggc atcgctgcat tacgaaccgc ccgtcagcaa
     9421 tatgatacgc gcgctgaagc acttggctga tttgggcatg gcgcagccgc tggcagacct
     9481 gacgatgcag aatccgcccg accggctttc agacgaatgt ttcgatttcg tcctacccgt
     9541 tccgctaagc agggagcggc tgctgcaacg cgggttcaac caaagcgaga gcatcgtcgg
     9601 gttgttggcg caacgctacg gctggcagat actgccccga cacaccgtat tccgacacca
     9661 ccgcccgccg caaagcacgc tcaaaggcgg cgaacggcgg cgaaacatca aaaacgcctt
     9721 tgaaatccgc acaccgatac cggaaaactg taatattttg ttaatcgacg atgtctttac
     9781 caccggcgcg acgctggacg aattggcaaa gacgctgaaa aaatcgggcg caaaccgaat
     9841 ctgctgctgg acgctggcac gcacgccaat gaaaaaataa ctaaattttt tgacacccca
     9901 tccgccttat ggcaaggtta cgcgcctata agtgattgat atttatgctt accatcgttt
     9961 tatatcagcc ggaaatcccc ccgaacacgg gcaacatcat ccgcctgtgc gccaataccg
    10021 gcgcggattt gcaccttgtc aaaccgctcg gcttcccatt ggattccgcc aaaatgaaac
    10081 gcgccgggct cgactaccac gaattcgcca gcctgacggt gcacgaaaac ttcgacgact
    10141 gcctcaagtc gctggcaggc cgacgtattt tcgccctgac caccaaaggc acggcgcgcc
    10201 ccgatgaaac cgcgtttcaa aaaggcgacg ttttactgtt cgggccggaa acgcgcgggc
    10261 tgcctgccga catcctcgac agcctgcccg ccgcgcaaaa aatccgcctg ccgatgcggc
    10321 ccggcagccg gagtatgaac ctttccaaca ccgtctccgt gattctcttt gaagcgtggc
    10381 ggcaacacgg ttacgcaggc ggcgtttgaa cgcaggttca tgccgtctga aaaccgttcg
    10441 gacacattcc gaaccgctgc cgcagcgtgc ggcggttcgg aacgcgtccg tagggcataa
    10501 tgcccatccg tcttcaactc aagaacggaa cacattttga ctttaacccg aaaaaccctt
    10561 ttcctcctca ccgccgcgtt cggcacacac tcccttcaga cggcatccgc cgacgcagtg
    10621 gtcaaggcgg aaaaactgca cgcctccgcc aaccgcagct acaaagtcgc cgaattcacg
    10681 caaaccggca acgcctcgtg gtacggcggc aggtttcacg ggcgcaaaac ttccggcgga
    10741 gaccgctacg atatgaacgc ctttaccgcc gcccacaaaa ccctgcccat ccccagccat
    10801 gtgcgcgtaa ccaacaccaa aaacggcaaa agcgtcatcg tccgcgtcaa cgaccgcggc
    10861 cccttccacg gcaaccgcat catcgacgta tccaaagccg ccgcgcaaaa attgggcttt
    10921 gtcagccaag ggacggcaca cgtcaaaatc gaacaaatcg tcccgggcca atccgcaccg
    10981 gttgccgaaa acaaagacat ctttatcgac ttgaaatctt tcggtacgga acacgaagca
    11041 caagcctatc tgaaccaagc cgcccaaaat ttcgccgctt cgtcatcaag cccgaacctc
    11101 tcggttgaaa aacgccgtta cgaatacgtt gtcaaaatgg gcccgtttgc ctcgcaggaa
    11161 cgcgccgccg aagccgaagc gcaggcacgc ggtatggttc gggcggtact gacctccggt
    11221 tgacggttat tcgatacctt attaatatcc ccatttttca acccctgatt tacaagggca
    11281 aatatgaaca tcaaacacct tctcttgacc gccgccgcaa ccgcactgtt gggcatttcc
    11341 gcccccgcac tcgcccacca cgacggacac ggcgatgacg accacggaca cgccgcacac
    11401 caacacggca a
//
LOCUS       DBJGGI010000066        11123 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.066, whole genome shotgun sequence.
ACCESSION   DBJGGI010000066 DBJGGI010000000
VERSION     DBJGGI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11123
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.066"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(41..211)
                     /locus_tag="ACSIGJ_001723"
     CDS             complement(41..211)
                     /locus_tag="ACSIGJ_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0043448 - alkane catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HHQ1330541.1"
                     /translation="MAQYMCGPCGWIYDEELGDPEHGIAPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
     gene            complement(344..1426)
                     /locus_tag="ACSIGJ_001724"
     CDS             complement(344..1426)
                     /locus_tag="ACSIGJ_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HHQ1330542.1"
                     /translation="MNAQTLLANVAELVKTKLKPLVDDIDRKGYYPEAFMRELGAIGA
                     VGIEAEGGNGLGLATRIAVLREIGKECGATSFSAWCQAACAWYLHQTPNQAVKDKYLA
                     DILQGKVLAGTGMSNTVKHLAGIEKHNLQAECVDGGYKVNGALPWVSNIGEDHIWANT
                     AQIGGGYVMFITGGQREGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPEQFA
                     GYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANIVNAEVNSYLDDGYDSLKARLDGAW
                     AETERLAGLAWDNTPDNLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQRRVG
                     EAMFVAIVTPAIKHLCKEIAAIEAAK"
     gene            complement(1564..2112)
                     /locus_tag="ACSIGJ_001725"
     CDS             complement(1564..2112)
                     /locus_tag="ACSIGJ_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HHQ1330543.1"
                     /translation="MARLTVHTLETAPEAAKPRVEAVPKNNGFIPNLIGVLANAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIRIIAARTNQCGFCVAGHTKLATLKKLLSEQSLNAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDDELKAFFDAGYNRQQAVEVVMGVALA
                     TLCNYANNLAQTEINPKLQAYA"
     gene            complement(2252..2623)
                     /locus_tag="ACSIGJ_001726"
     CDS             complement(2252..2623)
                     /locus_tag="ACSIGJ_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HHQ1330544.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     MTGAMFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGG"
     gene            complement(2808..4499)
                     /gene="dld"
                     /locus_tag="ACSIGJ_001727"
     CDS             complement(2808..4499)
                     /gene="dld"
                     /locus_tag="ACSIGJ_001727"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HHQ1330545.1"
                     /translation="MNASQLISSLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQKKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKVSFLPKHFSDKAMQFVSKFLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFSHHGGAFFECNAEETQAAMLHRFAVASAAIRYRSVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNGCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGIGKTSKKKNWAE"
     gene            complement(4596..5015)
                     /locus_tag="ACSIGJ_001728"
     CDS             complement(4596..5015)
                     /locus_tag="ACSIGJ_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330546.1"
                     /translation="MKKLLMITLTGMLAACSTGVNVGRLMVEMPQGERSVVVQVPATN
                     NPLSDAVAVGMIKTSGSPSASNMIEMLGADNINVGVAGGSQMFNKATALYSLNHAKKV
                     GNNVSVYMTGDSESDKADLENAANAKNIKLHYFFNQK"
     gene            complement(5238..5334)
                     /gene="ffs"
                     /locus_tag="ACSIGJ_001729"
     ncRNA           complement(5238..5334)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACSIGJ_001729"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5595..9428
                     /locus_tag="ACSIGJ_001730"
     CDS             5595..9428
                     /locus_tag="ACSIGJ_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HHQ1330547.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     QQVDVLIAAAEKAVERFDGSFDETRQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAVPLDANSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWNMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGINIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVAAVKARWDWLLAHLDAPLADYKSRYGTA
                     VHAAPEAKDDESCFTAFRDFRLRVSVKADVMKPLAEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLTDEDLQPFWNYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     SMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIHNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYSDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            9505..10506
                     /locus_tag="ACSIGJ_001731"
     CDS             9505..10506
                     /locus_tag="ACSIGJ_001731"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HHQ1330548.1"
                     /translation="MIDGQTTEPKQKTRIIPAPMRGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAD
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTGKMRLGYEDKS
                     PALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIHDTVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSTWIGQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEMDV"
     gene            complement(10567..10749)
                     /locus_tag="ACSIGJ_001732"
     CDS             complement(10567..10749)
                     /locus_tag="ACSIGJ_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330549.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPKAGEKRQ"
     gene            complement(10922..11053)
                     /locus_tag="ACSIGJ_001733"
     CDS             complement(10922..11053)
                     /locus_tag="ACSIGJ_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330550.1"
                     /translation="MSETAKIKLNDRPENAEKPNEKVELPIVDNDKKGGHGEGGCCG"
BASE COUNT         2466 a         3051 c         3034 g         2572 t
ORIGIN      
        1 cggaacacgc cgagggattt tccaaggggt gcggctgccg ctatatcacg aaatccaaca
       61 ggtagaaatc ttctttgccc acgccgcatt cggggcattt ccagtcgtcg gggatgtctt
      121 caaactttgt cccgggggcg atgccgtgtt ccgggtcgcc gagttcttca tcgtaaatcc
      181 agccgcaggg gccgcacata tattgagcca tttgtgtttc cttgtttgta ccgtgctttt
      241 ttgcagcggg ccgccggggt ttggttcgga tgccgtctga agcgtgccgc cgcatctgtc
      301 ccgccttggg cggtttgtgc ttcagacggc attttccgcc tgtttatttt gcggcttcga
      361 ttgctgctat ttctttgcag aggtgtttga ttgcgggggt tacgatggcg acaaacatcg
      421 cttcgccgac gcgcctttgg gcgggactcc gcatcaggta gcctttcgcg ccggaatgca
      481 gggcggcgga ttgcgcagcg gcaagggcca gtacggcggc ggcttcgcgc agcttgaggg
      541 tggcgaggtt gtcgggcgtg ttgtcccacg ccaatccggc gagccgttcg gtttctgccc
      601 acgcgccgtc cagccttgct ttgaggctgt cgtagccgtc gtcgagatag ctgttgactt
      661 ctgcattgac gatgttggcg aggcggatga tgccgaggct gccgtctatc acgcccgcgc
      721 cgatgccgat ttgcaggagg ataaagcctg ctttgatgct ttggatgtag ccggcaaact
      781 gttcgggcgc ggcgatgatg tcttcgtcgg ggataaatac gtctttgaag ttcaggctga
      841 aggtgcgcgt gccttcgagg gcgcaaaatt cggggcagtt ttgcaggctt acgccttccc
      901 tttgcccgcc tgtgatgaac atgacgtagc cgccgccgat ttgggcggta ttcgcccaaa
      961 tgtggtcttc gccgatgttg gacacccacg gcagcgcgcc gttgactttg taaccaccgt
     1021 ccacgcactc ggcttgaagg ttgtgttttt caatgccggc aaggtgtttg acggtattgg
     1081 acattccggt gcccgccaat actttgcctt gcaggatgtc ggcgaggtat ttgtctttga
     1141 cggcttggtt gggcgtttgg tgcagatacc acgcgcaagc cgcctgacac cacgcgctga
     1201 aggaggtcgc gccgcattct ttgccgattt cgcgcaagac ggcgattcgt gttgccaagc
     1261 ctaagccgtt gccgccttcg gcttctatac cgactgcgcc gattgcgccg agttcgcgca
     1321 taaatgcttc ggggtagtat cctttgcggt cgatgtcgtc gacgaggggt ttgagcttgg
     1381 ttttgacgag ttcggcaacg ttggcaagca gggtttgggc gttcatcttt gttccttaag
     1441 gtttttgggg ggaaatcggg ggcacgcctg atgcgcggct gcttttcccg ccgcttgggc
     1501 ggcttgtttt tgtttgaggg ggtgtttttg aaaaaccgcc tgcattcagg cggttttgtt
     1561 tgcttaggcg tatgcctgca atttggggtt gatttcggtt tgggcgaggt tgttggcgta
     1621 gttgcacaaa gttgccaagg ctacgcccat tacgacttcg actgcctgct gccggttgta
     1681 gcccgcatcg aaaaatgctt tgagttcgtc gtcggatact gcgccttttt tcgccattac
     1741 ggcttgggtg aaggcggcaa gcgcgccgag tttggcatcg tcaaattcac ctgccgccaa
     1801 agcgcgggcg gcattgaggg attgctcgga caggagtttt ttcagggttg cgagtttggt
     1861 gtgccctgcc acgcagaagc cgcattggtt ggtgcgtgcg gcaatgatcc ggatgacttc
     1921 gacttcgccg gcggtcaggc tgttggcggc gttgagcttg ccgacttctt ggtaaaacgc
     1981 caaggcttcg ggggcgtttg ccaatacgcc gatgaggttg gggataaagc cgttgttttt
     2041 gggtacggcc tctacgcgcg gtttggcggc ttcgggggcg gtttcgaggg tgtgtacggt
     2101 taaacgtgcc attttctttc cttgttcgca aatattgggg acgggcgcat ggtacgcatt
     2161 tcggaacgga ataggaaaga ctgtgccaca aacaaaaagt tataagaaat gccgcctgaa
     2221 gcctttcaga cggcattgcc gggaaacacg cctacccgcc cccgcgtccg aaatattgcg
     2281 ccaatgcggc ttgggggttg gcggcttcga ctgtttcggg caattcggta tagccgcgcg
     2341 ataaaaggtg gcgtgcataa aggctgttgc cctttgcgcc gaaccagacg gaaaccgcca
     2401 agcccaatac gttaaacgcc gagtctgtgg cgggcgtgtc gccgtaaacc agttgggcaa
     2461 acaccgccaa cacgaacatc gcgcccgtca tccccagccc caaaccccac attcttttga
     2521 aacacgccca cagcgtgccg aacaaaagac ccggccacga ccagccttgt ttgacggctt
     2581 ggggcggcag ggcgggatgg gtgtagattt tgtatggttt catcgtgttt ccttttcggt
     2641 tgaaaccctg ccctttggga aggtaggatc agactttatt tgtgagacgc gcgcccgtgt
     2701 gcagacatac ggcttgaaca tgcccgtcgg ggaagacaga tccgacaatg ccgtctgaac
     2761 ggtgttttgc cttcagacgg cattgcctgc cttcaaagcg gacgcgctta ttccgcccag
     2821 tttttctttt tgctggtttt gccgataccc gggttgaagc tgttggtcgg gtcgagtttg
     2881 cggtaaaact gtttgagcgc gggcttggct tcatacaaat ggccgacgtt gtgttcggcg
     2941 ggatattgcg cgccgcgttg gtctaagaga tgtagcattt cgtgttccaa cgccatacag
     3001 ccgttgcctt ttttgatgat gtaatcctga tggaaaacgt ggcacataaa atgcccgtaa
     3061 tacaatttat ggatgatttt attgtcgatt tccggcggca gtttttcaaa ccagtcgcgg
     3121 tcgtcgcggc gcagggcgat atccagcgcg accaagtcct ccacttcgtc gtcgtgcacg
     3181 gaacggtagc ggatggcggc ggaagcgacg gcgaaacggt gcagcatcgc ggcttgggtt
     3241 tcttcggcgt tgcactcgaa aaacgcgccg ccgtggtgtg aaaaatattc ttttaagaac
     3301 gcgcgcgcct catccacgcc ttttccgccc attttcagaa tcaggtggtg ttcgtatttg
     3361 tcgcggtaat cgcgcatgga tttgggcagg tggtcgggca ggaatttgct gacgaactgc
     3421 attgccttgt cggaaaaatg tttgggcagg aagctgactt ttttgccgaa cctgtccacg
     3481 cgcgccttca agtcaaataa tttcggcagt tggtgcgtac cgaatttttt gatgacgtag
     3541 aacgtgtctt tgccgtacac gtcggcaatg tcgaaagcgt ggcggtggat gtattcgccg
     3601 gaaacgggca ggctttcaaa ttcgcccaag gcggcgcggc ggatgtcggt cagctcgttg
     3661 atgtcgttcg tgccgatgta gaacacggcg gtttgttttt cttgcgggaa ggtgtccaaa
     3721 cggacggcaa aaaccatcag cttgcccgcg cagcccgagg cttcgtaatg gcgggcgggg
     3781 tcggcattga aacgcgcggc ggtcggttcg tccacttggc ggacgtgttc gcaataggcg
     3841 tggtcatgtc ctttgcccgc gtcttgtttg atgtcttttt tctgataatg atgaccttgc
     3901 aggttggtca ggatttcttc gggcgtgtcg cccaagtcta tgcccaagtg gttgaccagt
     3961 tccaacctgc cctcttcgtt gatttgggcg aacaacgcca tttcggtgta ggccgggccg
     4021 cgctgtacca acgcgccgcc ggagttgttg cacacgccgc ctaaaacgga cgcgccgatg
     4081 caagaagagc cgatgaccga atgcggttct cgtcccaaag gtttcagcag cagttcaagc
     4141 tggttcaggg tcgagccggg caggcagacg acttgttcgt tgttgttgat ggtttggatg
     4201 atgttcatcc gcatggtgtt cacaatcacg atgtcgcggt cgtaatcgtt gccgtcgggg
     4261 gtcgagccgc cggtcagtcc ggtattcgcc gcctgcgtaa tcacaatcac gtccgcttcg
     4321 acgcaggcct gcagaatttt ccacatttcc aaaatgcttc ccgggcgcac caccgccaac
     4381 gccttgcctt cgccgaaacg gtagccttgg cggtattgtt cggttttcgc ggggtcggtg
     4441 atgatgtatt tttcgcctac ggtttgggtc aggctgctga ttaattgcga ggcgttcata
     4501 acactctcct atcgggataa gcggcggcgc aaatcccggt acgccgccgt atgttcaatt
     4561 tggatatttt ggtgcggcaa gttgcacgct gcaaattatt tttggttaaa gaaataatgt
     4621 aatttaatat ttttggcatt tgccgcgttt tccaaatcgg ccttgtcgct ttcgctatcg
     4681 cccgtcatat agacactgac attatttccg actttctttg catggtttaa ggaataaagt
     4741 gcggtcgcct tattaaacat ttggctgcct cccgccacgc cgacgttgat attgtccgcg
     4801 ccgagcattt caatcatatt tgatgccgaa ggcgatccgg atgttttaat cattccgaca
     4861 gccaccgcat cggaaagcgg gttattcgtc gcgggaacct gcacgacaac agagcgttct
     4921 ccctgcggca tttcaaccat caaccggccg acattgacac ctgttgaaca agctgcaagc
     4981 ataccggtga gggttatcat tagaagtttt ttcatttgcc tactccttta gtctttaatc
     5041 aaaccgccct gttcgtcata taacttgaaa ttgaaccata cttccatttt tatgaagccg
     5101 gttcttcatt ggcctgtatg gatacattgt aatgatgcgt tatggcaaag taaaggaaat
     5161 gccgtctgaa cggctttcag acggcatcgg tgcgatacgg gaacgccgga acatcgaagc
     5221 tccggcgttt caaatagggc ggcggaccaa acccccggca ctggcgcatc ggagtgggct
     5281 gctggcttcc gcccctgacc cggtgttccg atttgccatg cggggagacc cgcctcggag
     5341 aaacggcatt ataacgggtt ttgtgaaaaa ctcaaccgtt ttgatacggt catacgccgg
     5401 aaacactgtc taaaacttat atccgataat attgtcaaca atttttcaaa gcgtcatttt
     5461 gtttctatga gggtatttcc tgtttcggca ttgaaaaata ttaagaatta aactacatta
     5521 ttgcctttca aaatttgtct gcaaccgacc gttcggacaa cactcaaata aaaacaacca
     5581 aacacggaca cgccatgacc acgactaccg ccccgcagcg tattcgggaa attccctaca
     5641 actatacttc ttataccgac cgcgaaatcg tcatccgcct gttgggcgat gaagcgtggc
     5701 aaatcctgca agacctgcgc ggtcaacgca aaacaggacg ttcggcgcgg atgttgtttg
     5761 aagtgttggg cgatatttgg gtggtcgtgc gcaatccgta tctggtcgat gatttgctgg
     5821 agcatccgaa acgccgcgcc gcgttggtac gcgaaatgcg ccatcgctta aatgaaatcc
     5881 gcaaacgccg cgacgacaat cagcaagtgg atgtgttgat tgcggcggcg gaaaaggcag
     5941 tggagcgttt tgacggcagc tttgacgaaa cgcgccaaaa gcggcggcag attttggaac
     6001 gcttaagcaa aatcaccaag ccgcacaaca tcatgtttga cggcttggct cgcgtaacgc
     6061 acgttacaga tgcaaccgac tggcgcgtgg agtatccgtt tgtcgtcgtc aatcctgata
     6121 ccgaggcaga gattgcccct ttggtgcgcg ccttaatcga attggacttg gttattatcc
     6181 cgcgcggcgg cggtacgggt tataccggcg gcgcagtacc tttggacgca aacagcgcag
     6241 tcatcaatac ggaaaagctg gacaagcatc gcggcgtgga atacgttgag ctggcaggct
     6301 tggacggcaa acatccgatt atccggtgcg gcgcgggcgt ggttacgcgg cgggtggaag
     6361 aaaccgcgca tcaggcgggc ttggtgttcg ccgtcgatcc gacttccgcc gatgcgtcct
     6421 gcgtgggcgg caatgtggcg atgaacgcgg gcggcaaaaa agccgtgctg tgggggacgg
     6481 cgttggacaa cctcgcctac tggaacatgg ttaaccctca aggcgaatgg ctgcgtatcg
     6541 aacgcgtgcg ccacaatttc ggcaaaatcc acgacgaaga aactgccgtg ttcgacgtgc
     6601 acacgctgga ttcagacggc atcaacatcg ttaaaaccga acgcttggaa atccccggac
     6661 acaaattccg caaagtcggt ttgggcaaag acgttaccga caaatttttg agcggactgc
     6721 ccggcgtgca gaaagaaggt acggacggca tcattaccgg cgttgccttc gtattgcaca
     6781 aaatgccgaa atacacgcgc accgtgtgta tggagttttt cggcacggtc gccaccgcca
     6841 cgccttctat cgtcgaaatc cgcgattttc tgctcgcaca cgacagcgtg cggctggcgg
     6901 gtttggagca tttggactgg cgttatgtcc gcgccgtcgg ctacgccacc aaagcggcgg
     6961 gcaaaggacg accgaaaatg gtcttgctgg cggacgtggt ttcagacgac gaagccgccg
     7021 tagaggcagc cgccgaacac atctgcgaac ttgcccgcgc ccgtgacggc gaaggcttta
     7081 tcgccgtatc gcccgaagcg cgcaaaacct tttggctcga ccgcagccgc accgccgcca
     7141 tcgccaaaca caccaacgcc tttaaaatca acgaagacgt ggtgattccg ctggagcggc
     7201 tgggcgagta ttcggacggc atcgaacgca tcaacatcga gctttccatc caaaacaaac
     7261 tcaaactctg tgccgctttg gagcaatatc tttcgggcaa actccccatc gacaaaatgg
     7321 gcaccgacct gccgaccgcc gaactgttgg gcgaacgcgg caaacacgcc ctcgcccacg
     7381 ttgccgccgt caaagcgcgt tgggactggc tgctcgccca tttggatgcg ccgcttgccg
     7441 actacaaatc acgctacggt acagccgtcc acgccgcgcc cgaagccaaa gacgatgaaa
     7501 gctgcttcac cgccttccgc gatttccgcc tgcgcgtgtc tgtcaaagca gacgtaatga
     7561 agccgcttgc cgaaatcttc agcggcaaaa ccgataccaa aattatccaa ggcttgggca
     7621 aaatccacgc caaaaccgta cgcagccgcg tctttgtcgc cctgcatatg catgccggcg
     7681 acggtaacgt ccacaccaat attccggtca actcagacga tgccgaaatg cttcagacgg
     7741 cctaccgttc tgtggaacgc attatgaaaa tcgcccgttc gctcggcggc gtgatttccg
     7801 gcgaacacgg catcggcatc accaagctcg aattcctcac cgatgaagat ttgcaaccgt
     7861 tttggaacta caaaaaccaa gtcgacccca agcacacctt caaccgccac aaactgatga
     7921 aaggttcgga cttacgcaac gcctacacgc cgtctttcga gctgttgggc gcggaatcgc
     7981 tgattatgga aaaatcagac ctcggcacga ttgccgattc cgtcaaagac tgcctgcgct
     8041 gcggcaaatg taaacccgtc tgctccaccc acgttccgcg cgccaacctg ctgtacagcc
     8101 cgcgcaacaa aatcctcggc gtgggtttgc tgaccgaggc cttcttatac gaagaacaaa
     8161 cccggcgcgg cgtttccgtc aaacattttg aagaactgat ggacatcggc gaccactgca
     8221 ccgtgtgcca ccgctgcgtc aaaccctgcc ccgtcaacat cgacttcggc gacgttaccg
     8281 tagccgtccg caactacctt gccgattccg gccacaaacg ctttgcgcct gcggcttcaa
     8341 tgggtatggc gtttttgaac gccaccggtc cgaaaaccat caaagccctg cgcgccgcga
     8401 tgatacagac cggcttcccc gcgcagaact ttgcctacaa aatcggcaag cttcttccga
     8461 tcggcacgaa aaagcaaaaa gccgaaccca aggcaaccgt cggcaaagcc ccgattaaag
     8521 aacaggttat ccatttcatc aaccgcccac tgcccaaaag cgtacccgcc aaaacaccgc
     8581 gctccttatt gggcatcgaa gacggcaaaa gcatccccat catccacaac cccgccgcgc
     8641 ccgaagatgc cgaagccgtg ttctacttcc ccggctgcgg ttcggagcgt ttgttcagcc
     8701 aaatcggact cgccgtccaa gccatgctct ggcacgtcgg cgtacaaacc gtcctgccgc
     8761 ccggctatat gtgttgcggc tatccgcaag acgctggcgg caacaaggca aaagccgaag
     8821 aaatgagcac caacaaccgc gtcgccttcc accgtatggc gaacaccctc aactacctcg
     8881 acatcaaaac cgtcgtcgtc agttgcggca cttgttacga ccagcttgaa aaataccgct
     8941 tcgaggaaat cttccccggc tgccgcatca tcgacatcca cgaatacctg ctcgaaaaag
     9001 gcgtgaaact cgacggcgtg aaaggtcagc aatacctcta ccacgacccc tgccacaccc
     9061 cgattaaaac catgaacgcc acccaaatgg ccagcagcct gatggggcag aaagtcgttt
     9121 taagcgaccg ctgctgcggc gaatccggta tgttcgccgt caaacgcccc gacatcgcca
     9181 cacaggtcaa gttccgcaaa caagaggaaa tcgagaaaaa cctcaaagag ctgccgcagg
     9241 gcgaacccgt caaaatgctg acctcctgcc ccgcctgcct gcaaggactg agccgctatt
     9301 ccgacgacaa caatatgcct gccgactaca tcgtcattga aatggcgaaa cacatcctcg
     9361 gcgaaaactg gctggatgag tttgtaaaaa aagccaacaa cggcggcgta gagaaagtgt
     9421 tgctataaca acggacacgg aaatgccgtc tgaacgccga aagccttcag acggcattgt
     9481 ttgaatcaaa tacctacccg aatcatgatt gacggacaaa caaccgaacc caaacaaaaa
     9541 accagaatca taccagcccc catgcggggg ctggtagacg atgtgatgcg cgacctgctg
     9601 acacgtatcg gcggctacga cgaatgcgtc agcgaatttg tacgcattac ccataccgtg
     9661 cattcacgat ccatatggtt aaaatatgtc cccgaaatcg ccaacggaaa caaaacgttt
     9721 tccggcacgc cctgcaccgt ccaacttctg ggcagcgatg cggacaatat ggcggcaaac
     9781 gcattggaag ccgtccgatt cggcgcggac aaaatcgatt tgaacttcgg ctgccccgcg
     9841 cccaccgtca acaaacacaa aggcggcgca attcttctga aagagccgga actgatattc
     9901 cacatcgtca aaacgctgcg cggacgtttg cctgcacata ttccgctgac ggggaaaatg
     9961 cggctaggct atgaagacaa aagccctgct ttggaatgcg cctgtgcgat tgccgaaggg
    10021 ggcgcgtgcg gactgaccgt gcacgcgcgc accaaagccg agggctacga acccccggcg
    10081 cattgggaat ggataaggaa aatccacgat accgtcaata ttcccgttac cgccaacggc
    10141 gacgttttca gcctgcagga ctatatcggc atcaaaacaa tcagcggctg caacagcgtg
    10201 atgctcggtc gcggtgcggt catccgcccc gatttggcgc gccaaatcaa gcaatacgag
    10261 aacggcggac cggtcaaaga cacggatttt gccgaagttt ccacatggat agggcagttt
    10321 ttcgagctgt gcctgacaaa agaggcaaac aacaaatacc cgatcgcccg tcttaagcaa
    10381 tggctgggca tgatgaaaaa aacgttcgac ccggcgcaaa cgctgttcga ccgcgtccga
    10441 acggttaaag atgcggatga agtgcgccga atcctgaacg catttgagca tgagatggat
    10501 gtttgatgtc tcccgcgctt caaagcaaat gccgtctgaa aacctttcag acggcatttg
    10561 ttatctttat tgccgttttt cccctgcttt cggtgtattt ttgtttttca gtttcgcccc
    10621 caaacccaaa cgtttttcat aatccgattg cggggtatcg tcttcctgca taccgaacgc
    10681 gccggcattg acccacagcg acagcagcgc gacgacaaag gcgcaaaagc cgattacata
    10741 ccaaaacatt gcccctccaa atttgttaaa atcatatcaa atacagtgcc gaatttatca
    10801 caagcgcacg agcaaataaa tcttctgacc gcagtatttc attcatttac cttgaaaaca
    10861 aaaaagggca cctgaaaatt ttgcgaaact tttcagatgc cctgctttgc cgaaaaacgt
    10921 atcagccgca gcaaccgcct tcgccgtgtc cgcctttttt gtcgttatcg acaatgggca
    10981 gttcgacttt ttcgtttggt ttttctgcat tttcgggccg gtcgttgagt ttgattttcg
    11041 cggtttcgct catttcctta cctcatttgg aaaacggttt gaaagattca taaatatagc
    11101 atgtttaatc cgaatctgaa aag
//
LOCUS       DBJGGI010000067        10981 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.067, whole genome shotgun sequence.
ACCESSION   DBJGGI010000067 DBJGGI010000000
VERSION     DBJGGI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10981
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.067"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(25..1053)
                     /gene="trpD"
                     /locus_tag="ACSIGJ_001734"
     CDS             complement(25..1053)
                     /gene="trpD"
                     /locus_tag="ACSIGJ_001734"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HHQ1330551.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMCEFASKVPLEDADGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNIAASLSDGISAAREGIDSGRAKAKKEEF
                     VGFTRQFA"
     gene            complement(1117..1707)
                     /locus_tag="ACSIGJ_001735"
     CDS             complement(1117..1707)
                     /locus_tag="ACSIGJ_001735"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HHQ1330552.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVEAMRHFAGRLPIMGVCLGHQTIGEAFGGDVVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIERGTLPDCLEITAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTERGHDMLNNFLVEFQNFKPQKI"
     gene            2118..4394
                     /gene="znuD"
                     /locus_tag="ACSIGJ_001736"
                     /gene_synonym="tdfJ"
     CDS             2118..4394
                     /gene="znuD"
                     /locus_tag="ACSIGJ_001736"
                     /gene_synonym="tdfJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118820519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc receptor ZnuD"
                     /protein_id="HHQ1330553.1"
                     /translation="MAQITLKPIVLSILLINTPLLAQAHETEQSVGLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGEAGLRLSSGNLEKLTSAGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGAAYSDRRDRYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDGDGAHAHTHNGKPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYHHDEKAGDAVENFFNNKTHNARIELRHQPIGR
                     LKGSWGVQYLGQKSSALSAIPETVQQPMLIDNNVRHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNQPLPDLGAHRQTARSFALSGNWYFTPHHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLAAYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDPYGKRPFIAQDDQNAPRIPAARLGFHLKTSLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            complement(4934..5713)
                     /locus_tag="ACSIGJ_001737"
     CDS             complement(4934..5713)
                     /locus_tag="ACSIGJ_001737"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HHQ1330554.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDVYLPVDAQAQVAIGDKVTGVKTVLARLPLTDS
                     QADPVSQAASVETAANPSAEQQQIEAAAAKIQAAVQDVLKD"
     gene            5867..8716
                     /gene="uvrA"
                     /locus_tag="ACSIGJ_001738"
     CDS             5867..8716
                     /gene="uvrA"
                     /locus_tag="ACSIGJ_001738"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HHQ1330555.1"
                     /translation="MCNHHPRHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILGPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     HHYKFDVNVAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCEKSVTGQYLGGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGKVIAKGSPEEVAKVKGSYTGKYLKVVLK"
     gene            8734..9375
                     /locus_tag="ACSIGJ_001739"
     CDS             8734..9375
                     /locus_tag="ACSIGJ_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco29kI family restriction endonuclease"
                     /protein_id="HHQ1330556.1"
                     /translation="MNNNFDRSKHIYHNEAFVELVKDAIRFFHGTPVHTLPPPKQFKG
                     AGVYAIYYIGNNPLYKQYADWNRLSYNAPIYVGKAVPKGWRQARNSDNALNQSTELFH
                     RLKEHSRSIAAVSDLDPSDFMCRFVIFEGAGSDMIGTIEAALIKLHKPLWNSCVDGFG
                     NHDPGKGRYEQAKSDWDVLHSGRVWADRLNGIPNSYESILENINTHLEIIKRK"
     gene            9379..10512
                     /locus_tag="ACSIGJ_001740"
     CDS             9379..10512
                     /locus_tag="ACSIGJ_001740"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HHQ1330557.1"
                     /translation="MKSLEIFSGAGGLAKGLELAGFQHASFIELNKDACNSLRSNFNP
                     KLVYQGDVADFDLSSQEGIEVIAGGPPCQPFSLGGKHLAHEDRRDMFPHAVRYVEYYR
                     PKAFIFENVKGLLRKSFADYFEYILLRLTYPNLGILQNEDWKGHLTRLKEIEFNLYKG
                     IKYKVSYQLLNAADYGVPQKRERVVIVGIRADLDIDWKFPKRTHSEDRLNWEKYVTGE
                     YWEKHNEPKRFNKDIAEKLQKKYGIFEPEKKPWQTVRDTLSDIPHPLGNHKITGHEYR
                     DGARIYPGHTGSGIDEPSKTIKAGGHGVPGGENMIRYDDGTVRYFTSYEAKLLQTFPE
                     EFVISGAWGEAMRQIGNAVPVKLSEILGKHLMGVLSEKSSLHN"
BASE COUNT         2900 a         3088 c         2552 g         2441 t
ORIGIN      
        1 ttgctttata tcaagtttgc cggcttaggc gaattgccgt gtaaaaccga caaactcctc
       61 ttttttcgcc ttcgccctac ctgaatcgat gccttcccgt gcggcagata tgccgtctga
      121 aagcgaagca gcgatatttc cggcatataa ggcggcggcg gtattaagca atacgatatc
      181 gcgcgccgcc ccttctttcc cgtccagtac ctcgttcatt ttcaacaaag attcctgagt
      241 attggcaact ttgatttcat ccaaattgcg gcgggtttcg ataccgaaat cttctgggcg
      301 gatgtcgtat tcgctgattt ttccgtcttt gagttcggca acgcgcgttt tgcccgtcag
      361 tgtaatttca tccaaaccgc cctccccgca aacgaccaaa acgtgttttg agccgagttg
      421 ttgcaagacc cgcgacaaaa tgccgcacaa atcggtgtgg aacacgccca aaagctggtt
      481 cggcgcgccc gcaggattgg ttaacggacc caatatgttg aaaatgcttc ggaaaccgag
      541 cgaacggcgc accggggcga catggcgcat ggcactgtgg tgattgggtg cgaacataaa
      601 ccctataccg gtctgcctga tactttgggc aatctgttcg ggagtcaggt tgaggtttgc
      661 gcccatttgc tccatcacgt cagccgcacc gctggaggaa gagaccgacc ggcctccgtg
      721 tttggcaacc tttgcgcctg ccgctgcagc aacaaacatc gaagtcgtcg aaatattgaa
      781 ggttttcgcg ccatccccgc ccgtaccgac gatatcgacc agcccgtccg catcctccag
      841 cggcactttt gacgcaaact cgcacatcac ggcggcggcg gcggtgattt cggaaacggt
      901 ttcaaccttg atacgcaagc cggtcaaaat cgccgcaatt tgttcgggcg gcacttttcc
      961 gctcatcatt tggcgcatca agtcggtcat ttcatcgtaa aacaactcgt tattgctgat
     1021 taatcgttcg atagcctgtt gcggtgtaat cattttttgt cctccgttca atattcggac
     1081 gaaaatgccg cccgaagggc ttcagacggc atcacgtcag attttttgcg gtttgaagtt
     1141 ttgaaattca actaaaaaat tgttcaacat atcatgtccg cgttcggtca agagggcttc
     1201 ggggtggaac tgcacgccct cgacggcata ttccttatgg cgcacgccca taatttcgcc
     1261 gtcttccgtc cacgccgtga tttccaagca atccggcagc gtgccgcgtt cgataacgag
     1321 gctgtgataa cgcgtacagg taaccggatt gggcaaaccc ttaaacatac ccttgcccga
     1381 atgggacacg ggcgacacct taccgtgcat caaggttttt gcccgtacca catctccacc
     1441 gaacgcttcg cctatcgtct gatgcccgag gcacacgccc ataatcggca gccggccggc
     1501 aaaatggcgc atggcttcta ctgaaatacc cgcctcctta ggggaacacg gtccgggacc
     1561 gataacgaga tattgcggat tcaatgcctc gatttcctcc aacgtaatat catcgttgcg
     1621 gcgcacggcg acttcctgcc ccaattctgc gaaatactgg acgatgttgt aagtaaaact
     1681 gtcgtaattg tcgataaaca aaagcatttt gtgattaacc tattgattta taatttgttt
     1741 attattaact gcatttccga tacccccatc cataccccct tatatcttag cgtgcccgat
     1801 gcgccctcgt gaatctggcg cagagcctcg cgttctgaca agtacgccat tttgccacct
     1861 tttcgaccgt tcgggaacgt accgttttta tgccctcata aattttggtt atccgatagc
     1921 taacatatag cggcgttcgt gttcacatca ttaacttccc taaaattagg gttaatacaa
     1981 attaacgcca ttaaatatca caaccgtgtc atttgaaaaa cttttgccta tgccctctga
     2041 gtattctcaa aatcttatga tataaaaaat gcaatgttat ataataacaa acttttgaaa
     2101 tgcgacgaga agccaaaatg gcacaaatca cactcaaacc cattgtttta tcaattcttt
     2161 taatcaacac acccctcctc gcccaagcgc atgaaactga gcaatcggtg ggcttggaaa
     2221 cggtcagcgt cgtcggcaaa agccgtccgc gcgcgacttc ggggctgctg cacacttcga
     2281 ccgcctccga caaaatcatc tccggcgata ctttgcgcca aaaagccgtc aacttgggcg
     2341 acgctttgga cggcgtaccg ggcattcatg cctcgcaata cggcggcggc gcatccgctc
     2401 ccgttattcg cggtcaaacg ggcagacgga ttaaagtatt gaaccatcac ggcgaaacgg
     2461 gcgatatggc ggacttttct cccgatcacg ccattatggt agataccgcc ttgtcgcaac
     2521 aggttgaaat cctgcgcggg ccggttacgc tcttgtacag ctcgggcaat gtggcggggc
     2581 tggtcgatgt tgccgatgga aaaatccccg aaaaaatgcc tgaaaacggc gtatcgggcg
     2641 aagccggatt gcgtttgagc agcggcaatt tagaaaaact gacatccgca ggcatcaata
     2701 tcggactggg caaaaacttc gtgctgcata ccgaaggctt gtaccgcaaa tcgggcgatt
     2761 acgccgtacc gcgttaccgc aatctgaaac gcctgcccga cagccatgcc gattcgcaaa
     2821 cgggcagcat cgggctgtct tgggtaggcg aaaaaggctt tatcggcgca gcatacagcg
     2881 accgtcgcga ccgctacggc ctgcctgccc acagccacga atacgatgat tgccacgccg
     2941 acatcatctg gcaaaagagt ttgatcaaca aacgctattt gcagctttat ccgcacttgt
     3001 tgaccgaaga agacatcgat tacgacaatc cgggcttgag ctgcggcttc cacgacggcg
     3061 acggtgcaca cgcacacacc cacaacggca aaccgtggat agacctgcgc aacaaacgct
     3121 acgaactccg cgccgaatgg aagcagccat tccccggttt tgaagccctg cgcgtacatc
     3181 tgaaccgcaa tgactaccac cacgacgaaa aagcaggcga tgcagtagaa aacttcttca
     3241 acaacaaaac acacaacgcc cgtatcgagt tgcgccacca acccataggc cgtctgaaag
     3301 gcagctgggg cgtgcaatat ttgggacaaa aatccagcgc gctttccgcc attcccgaaa
     3361 ccgtccaaca accgatgttg attgacaaca atgtccgcca ttacagcttt ttcggtgtag
     3421 aacaggcaaa ttgggacaac ttcacgcttg aaggcggcgt acgcgtggaa aaacaaaaag
     3481 cctccatccg gtacgacaaa gcattgattg atcgagaaaa ctactacaac cagcccctgc
     3541 ccgacctcgg cgcgcaccgc caaaccgccc gctcgttcgc actttcgggc aactggtatt
     3601 tcacgccaca ccacaaactc agcctgaccg cctcccatca ggaacgcctg ccgtcaacgc
     3661 aagaactgta cgcacacggc aagcacgtcg ccaccaacac ctttgaagtc ggcaacaaac
     3721 acctcaacaa agagcgttcc aacaatatcg aactcgcgct gggctacgaa ggcgaccgct
     3781 ggcaatacaa tctggcagcc taccgcaacc gattcggcaa ctacatttac gcccaaacct
     3841 taaacgacgg acgcggcccc aaatccatcg aagacgacag cgaaatgaag ctcgtgcgct
     3901 acaaccaatc cggtgccgac ttctacggcg cggaaggcga aatctacttc aaaccgacac
     3961 cgcgctaccg catcggcgtt tccggcgact atgtacgagg ccgtctgaaa aacctgccgt
     4021 ccctacccgg cagggaagat ccctacggca aacgtccctt catcgcgcag gacgaccaaa
     4081 acgccccccg cattccggct gcgcgcctcg gcttccacct gaaaacctcg ctaaccgacc
     4141 gtatcgatgc caatttggac tactaccgcg tgttcgccca aaacaaactc gcccgctacg
     4201 aaacgcgtac gcccggacac catatgctca acctcggtgc aaactaccgc cgcaatacgc
     4261 gctatggcga gtggaattgg tacgtcaaag ccgacaacct gctcaaccaa tccgtttacg
     4321 cccacagcag cttcctctct gatacgccgc aaatgggccg cagctttacc ggcggcgtaa
     4381 acgtgaagtt ttaaaatcgg acaggcaaac aaaaaaggtt ttcagacggc attctgaaaa
     4441 cctttttttc tttttaatca acttgttagg aaatatggca aagacaagga acaaaccact
     4501 gtgccatcat tcccattttc tttgtttcat cattcttgct ttcacgccgt cattctcgca
     4561 aaagcagaaa aatcaaaagc aaaaacccaa gatcccatca ttcccgcgca ggcgggaatc
     4621 caattcattc ggtttcagcc gtttccgata aatcaccgta acgttaagtt cccggattcc
     4681 cactttcgtg ggaatgacgg cgtaaaggtt tctgtttttt aagttttagg taactaatga
     4741 gtcgtcattc ccgcgcaggc gggaatccaa ttcgttcggt ttctgttatt tctgataaat
     4801 caccgtaacg ttaagttccc ggattcccat tttcgtgaac ataacggtgt gaacattagc
     4861 aaaaattcaa aaaaccaaac ttcctcattc gtgtgaatga tggtactgat attttctatt
     4921 tcagttcgca aaattaatct ttcaacacat cttgcacagc cgcctgaatc ttagccgccg
     4981 ccgcttcgat ttgctgctgt tcggcagatg ggtttgccgc tgtttcaacc gaagcagctt
     5041 gtgaaacagg atcggcttga gaatcagtca gcggcaaacg cgccaatacg gtttttacgc
     5101 cggttacttt atcaccaatc gccacttgcg cctgcgcatc gacaggcaga tacacatcca
     5161 cgcgcgaacc gaagcggata aagccatagc gttcgccgcg ggacagtttc gcacctgctt
     5221 gggtgtaaca caaaatacgg cgtgccacca gaccggccac ttgaacaaaa gtaatttcac
     5281 gaccggaagc cgtagtcgcc aaaaccgcgt tgcgttcgtt ttccgtgctg gctttgtcca
     5341 aatccgcatt cacgaatttg cctttgttat agaccacttt cgttaccgta caatcggcag
     5401 gcgatttttg cgaatgcacg ttgaacacgt tcataaaaat actgattttc aaagcatcga
     5461 catcacgata cggatcgcgt gcgcgctcga ccaccacgat acggccgtca accggactca
     5521 acaccgcttc aggattttgc ggaatttcac gcgcagggtc gcggaaaaac tgcaatgcaa
     5581 atacggtaaa cacccaaaac ggcaaagacc accagccgca gcacatcgac accagcaggc
     5641 tcaaagccaa accgccgccg ataatcggcc agccctcacg ggcgataatc gggtggggat
     5701 aaagacggtt catttgtcat ccttttattc aaatatatta aatagcgaca ttataacgga
     5761 atccgccgcg ttccgccgac aaatgccgtc tgaacgcggc ttcagacggc atccgagaaa
     5821 gtgctacaat atcggttttc ctttattttt tcaggcggca tccattatgt gcaaccatca
     5881 tccccgacat tcgcacgaca acgacaccat ccgcattcgc ggcgcgcgca cgcataattt
     5941 gaaaaacatc gatttggaca ttccccgcca caagctcgtg gtggtaacag gattgtcagg
     6001 cagcggcaaa tcatcgctgg cgtttgatac tttatatgcc gaagggcagc ggcgttatgt
     6061 cgagagcctg tctgcctatg cgcggcagtt tttgcagatg atggacaaac ccgatgtcga
     6121 tttgattgaa ggcctgtcgc ctgcgatttc catcgagcag aaatccacca gccacaatcc
     6181 gcgttccacc gtcggcacgg taacggaaat ccacgactac ctgcgccttt tatacgcccg
     6241 tgtcggcacg ccgtattgcc ccgaacacaa gctgccgcta tccagccaaa ccgtatctca
     6301 aatggtcgat gccgtattaa aactgccgga agatacgcgc gtgatgattc tggggccggc
     6361 ggtgcgcgag cgtaagggcg agtttgtcga tttctttgcc gacttgcagg cgcaaggctt
     6421 tgcccgtgtg cgcgtggacg gcgaagtcta tcagcttgac gaagtaccga agctggaaaa
     6481 aaacatcaaa cacaatatcg acgtcgtcat cgaccgcgtg aaagtgaaag ccgacatcaa
     6541 acaacgactg gcggaaagtt ttgaaaccgc gctgcgccac ggcaacgagc gcgcgctggc
     6601 gatggaaatg gacagcggcg aagaacattg gttttccgcg cgttttgcct gccccgtatg
     6661 ctcatacagc ctgcccgaat tggaaccgcg cctcttttcg ttcaacaacc cgatgggttc
     6721 ctgcccgact tgcgacggct tgggcaacac caatttcttc gaccccgaaa aagtagtcgc
     6781 ccatcccgaa ttgtcgctgg caaccggcgc gattgacggt tgggacaaac gcaaccaatt
     6841 ctatttccaa atgattcaat cgctggcgca tcattataaa tttgatgtca acgtcgcttg
     6901 ggaaactttg cctgaaaaag tcaaaaaagt cgtgttgcac ggctcgggca aagaagtcat
     6961 tgatttcacc tacctgtccg aacgcggcac caccttcaac cgcagccacg ccttcgaggg
     7021 catcatcccc aatctcgaac gccgctaccg cgaaaccgac agcgaaaccg tgcgcgaaaa
     7081 actgcgcgag taccaaaatc accgtgcctg cccgagctgc ggcggcgcac gtttgcgcaa
     7141 agaagcacgc tacgtttacg tcggcggcga accgttgcac gaagtctccg cctggccgct
     7201 gaccaaaacc caccgattct tcgaaacgct ggatttggac ggcaacaaaa aacagatcgc
     7261 cgaaaaaatc ctcaaagaaa tcaccgagcg gctcggcttc ttgattaacg tcgggctgga
     7321 ttacctgaat ttaagccgtt ccgccgaaac cctttccggc ggcgaagccc agcgcatccg
     7381 cctcgccagc caaatcggca gcggcctgac cggcgtgatg tacgttttgg acgaaccctc
     7441 catcggcctg caccagcgcg acaacgaccg cctgctcgcc accctcaaac gcctgcgcga
     7501 tttgggcaac agcgtaattg tggtcgaaca cgacgaagac gccatccgcg aagccgattt
     7561 cgtcgtcgat atgggccccg gcgcgggcga acacggcggc aacgtactga ttgccgacac
     7621 acccgaaaat gtcgccaaat gcgaaaaatc cgttaccgga caatacctcg gcggcaaaaa
     7681 atccattgcc gtgccgtctg aacgcacgcc cgtcaatccc gaccgaatgc tcgtcctcaa
     7741 aggtgcgcgc ggcaacaacc tcaaaaacgt caccctcgag ttgccgctcg gtttgattac
     7801 ctgcatcacc ggcgtatccg gcagcggcaa atccaccctg attaacgaca ccctcgccaa
     7861 aatcaccgcc cgcgaactca accgcgccca agaagaaccc gccccatacg acgacatccg
     7921 cggcctcgaa cacctcgaca aagtcatcaa cgtcgaccaa tcccccatcg gccgcacccc
     7981 gcgctccaac cccgccacct acaccggcct gtttaccccc atccgcgaac tcttcgccgg
     8041 cgtacccctc tcgcgcgaac gcggctacaa cgtcggcaga ttctccttca acgtcaaagg
     8101 cggccgctgc gaagcctgcc aaggcgacgg cgtaatcaaa gtcgaaatgc acttcctgcc
     8161 cgacgtgtac gtcccctgcg aagtctgcca cggcaaacgc tacaaccgcg aaaccctcga
     8221 aatccaatac aaaggcaaaa acatcagcca agtcctcgac atgacggtcg aagaagcccg
     8281 cgaatttttc gacgccgtcc ccaccgtatc gcgcaaactg caaaccctga tggacgtagg
     8341 tctcggctac atccgcctcg gccaatccgc caccaccctc tccggcggcg aagcccagcg
     8401 cgtcaaactc gccttggaac tctccaaacg cgacaccggc agaacgctct acatcctcga
     8461 cgaacccacc accggcctgc acttcgccga catcgccctg ctgctggaag tcataggccg
     8521 tctgaaaggc aaaggcaact cgattgtgat tatcgagcat aatctggatg taattaaaac
     8581 cgcggattgg attgtggact tagggccgga aggcggcgat ggtgggggta aggttatcgc
     8641 gaaaggtagc cctgaggaag tggcgaaggt taaggggagt tataccggga agtatttgaa
     8701 agtggttttg aagtaaacga acggatatag aaaatgaaca ataattttga ccgcagcaag
     8761 catatctatc ataacgaagc ttttgtagaa ctggttaaag atgccatacg cttttttcat
     8821 ggtacgcctg ttcatacact tcctccaccc aaacagttca aaggtgcagg ggtttatgcg
     8881 atttactata tcggtaacaa tcctttgtac aaacaatatg cggactggaa cagattatcc
     8941 tacaatgcgc caatttatgt tggcaaggcc gtccccaaag gctggcggca agcaagaaat
     9001 tctgataatg cgctaaacca atcaaccgaa ctgttccacc ggttgaaaga acacagcagg
     9061 agtattgccg ctgtttctga tttagatcct tccgatttca tgtgcagatt cgttatcttt
     9121 gaaggtgccg gttctgacat gataggcaca atcgaagctg ccctgattaa attacacaaa
     9181 cctctatgga actcttgtgt cgatggtttc ggaaaccacg accccggaaa aggccgttac
     9241 gaacaagcca aatcggattg ggatgtcctt catagcgggc gggtttgggc agataggcta
     9301 aacggcatac cgaatagcta tgagtccata cttgaaaata tcaacacaca tttagagata
     9361 ataaagagaa aataacctat gaagtctttg gaaatttttt ccggcgcggg cgggttggca
     9421 aaaggccttg aacttgcagg attccaacat gcatctttta ttgaactcaa taaagatgca
     9481 tgcaattctt taagaagcaa ctttaacccc aagcttgttt atcagggaga tgtggcagat
     9541 tttgatcttt ccagtcaaga aggcatagaa gtcattgcag gcggtcctcc ttgccagccc
     9601 ttttcattgg gcgggaaaca ccttgcccat gaagacagaa gggatatgtt tcctcatgcg
     9661 gtcagatatg tggagtatta ccgccccaaa gcttttatct ttgagaatgt aaaaggactt
     9721 ttaagaaaat cattcgctga ttattttgaa tatatcctac ttcggcttac ttatccgaat
     9781 cttggtattt tgcagaatga agattggaaa ggccatctga cacggctgaa agaaattgaa
     9841 ttcaatttat ataaaggaat caaatataag gtttcatatc aattgttgaa tgcagctgat
     9901 tacggagtgc cgcaaaaacg cgaacgcgtg gttatcgtag gtatccgggc cgatttggat
     9961 attgattgga aattcccaaa aagaacacat tccgaagaca ggttgaattg ggaaaagtat
    10021 gtaacggggg aatactggga aaaacacaac gaacccaaaa gattcaataa agatattgct
    10081 gaaaagttac aaaaaaaata cggtatattc gaaccagaaa aaaaaccttg gcaaacggta
    10141 agggatacct tgtccgacat cccgcatcct ttggggaatc ataaaattac aggacatgaa
    10201 tatagggatg gcgcaagaat ttatcccgga cacacaggaa gcgggataga cgaaccgtcc
    10261 aaaaccatta aagcaggtgg gcatggcgtt cccggcggag aaaatatgat tcgttatgat
    10321 gatggaacag tcagatactt taccagctat gaagcaaaac ttcttcaaac attccctgaa
    10381 gaatttgtca tttctggagc ttggggagaa gcaatgcgac aaattggcaa tgcggttcct
    10441 gtcaaattgt cggaaatttt aggcaaacat ctgatggggg tgttgtcgga gaaaagcagc
    10501 ctgcacaact aacccgccca ctgcgtgtgt gcggtacgca cgcggttgct tgattgacgg
    10561 tttgccgttt caacttttaa cttcgttgaa gctagtgctt tcagacggcc tccaatgtcg
    10621 tttggaaaca tcaggtccaa caaagttaaa ccttaaacaa caaaccattc cttctccaac
    10681 acccaaacca caaacaaaaa tcctatggcg cgttatcgca gaaacttcat tgccggcggc
    10741 acattctttt tcaccgtcaa acccgccgcc ccgaaatcgc gtctgcttgt cggacatatc
    10801 ggcttcgcgt gcggcttata tggatgtgca aaaacaatat ccctttgaaa ccgtcgccgt
    10861 atgcgtgctg ccgaaccata ttcacgccat ttggacgctg ccgcccgacg atgcggatta
    10921 ttccctactc cggcggctga ttaaaaccaa attctccgca tattcccctc atactaaaaa
    10981 a
//
LOCUS       DBJGGI010000068         9833 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.068, whole genome shotgun sequence.
ACCESSION   DBJGGI010000068 DBJGGI010000000
VERSION     DBJGGI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9833
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.068"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..208
                     /locus_tag="ACSIGJ_001741"
     CDS             <1..208
                     /locus_tag="ACSIGJ_001741"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330558.1"
                     /translation="PSPPPRGREWVSADFSVAGGLKNNLNLQPLISGRLKKDARNINS
                     GNFSGSLYCQADGTNAASVFSDNL"
     gene            365..928
                     /locus_tag="ACSIGJ_001742"
     CDS             365..928
                     /locus_tag="ACSIGJ_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HHQ1330559.1"
                     /translation="MAIELNGNWTKGFALDHHMQKSILLGYDELGHPHFDSKRSVIGE
                     WVYQLKYRNQTQNVSLLVDYILQKFSGLESINLIVPAPFTTERINQPVQLIAKELSDR
                     LNIPYSPILRKSSSHTPLKNIEEKSEKLEILNNSITIDNIDLSNKNILVIDDLFDSGA
                     TLEISTEKLFSKNARSVIVLAMTKTRG"
     gene            931..1479
                     /locus_tag="ACSIGJ_001743"
     CDS             931..1479
                     /locus_tag="ACSIGJ_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330560.1"
                     /translation="MRTIFFSGSRSISRLNPQIRERINNILSNNFDIVIGDANGADKA
                     IQEFLQEQDYANVHIYFSGKIYRNNVGNWQFVQVDSKGTGRVFYTAKDKKMAEIADYG
                     FILWDGKSIGSLNNIAELLQLNKPSLVYHSQTKEFFKIKSSADLENILSNIEDDVLAS
                     ILEKGNTFLKSYVTKQPSLIQE"
     gene            1486..4701
                     /gene="carB"
                     /locus_tag="ACSIGJ_001744"
     CDS             1486..4701
                     /gene="carB"
                     /locus_tag="ACSIGJ_001744"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HHQ1330561.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKADNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGRTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSEDKAEIRRELANPGPERMLFVADAFRAGFTPEEIHEI
                     CAIDPWFLAQIEDLMKEEKSVSDGQLQDLDYAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILSPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQAINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDYLGVYSVQELHGRLKNRN"
     gene            complement(4902..5942)
                     /gene="queA"
                     /locus_tag="ACSIGJ_001745"
     CDS             complement(4902..5942)
                     /gene="queA"
                     /locus_tag="ACSIGJ_001745"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HHQ1330562.1"
                     /translation="MDISDFDFTLPEHLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETVAAVEAAKARGNKAWAVGTTSMRALESAARATGYLKDGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVGAFSGMGHIRAVYRHAIEREYRFFSYGD
                     AMVLGRNEGGGL"
     gene            6059..6316
                     /locus_tag="ACSIGJ_001746"
     CDS             6059..6316
                     /locus_tag="ACSIGJ_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HHQ1330563.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENEHNFTVVSHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            6298..6759
                     /gene="accB"
                     /locus_tag="ACSIGJ_001747"
     CDS             6298..6759
                     /gene="accB"
                     /locus_tag="ACSIGJ_001747"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HHQ1330564.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAAAPVY
                     AAPVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAAFVEV
                     GQQVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            6873..8234
                     /gene="accC"
                     /locus_tag="ACSIGJ_001748"
     CDS             6873..8234
                     /gene="accC"
                     /locus_tag="ACSIGJ_001748"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HHQ1330565.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKGSLHVKL
                     ADESVCIGPAASAQSYLNIPAIIAAAEVTFADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDDAEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVLADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFIDEEARKKIGKACTDACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIASGLPLQYKQKDIKIEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQEGGVSIHYLEHWLEARKTK
                     QDK"
     gene            8338..9225
                     /gene="prmA"
                     /locus_tag="ACSIGJ_001749"
     CDS             8338..9225
                     /gene="prmA"
                     /locus_tag="ACSIGJ_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HHQ1330566.1"
                     /translation="MPYQQITVNVNDAVAERLADVLMEHGALSAAIEDACAGTQNEQA
                     IFGEPGMPTEQIWQQSKVIALFGEHDEAAAVIDAAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGCAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGRDNAEQNNVDAQFFLP
                     DSLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETDEGWARLSGVKR"
     gene            9243..9806
                     /gene="orn"
                     /locus_tag="ACSIGJ_001750"
     CDS             9243..9806
                     /gene="orn"
                     /locus_tag="ACSIGJ_001750"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HHQ1330567.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDDLLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWIPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPIAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPKAEAQ"
BASE COUNT         2517 a         2792 c         2496 g         2028 t
ORIGIN      
        1 ccccagccct cccccacggg ggagggagtg ggtttcggca gatttttcgg ttgccggtgg
       61 tttgaaaaat aacttaaatt tgcaaccgtt gatttcaggt cgtctgaaaa aggatgcacg
      121 aaatatcaac agcgggaatt tttcaggtag cctttattgt caagcagatg gaacaaacgc
      181 cgcgagcgtt ttttcagaca atctttgaac tcatcggtag agtgtgtgcc gcaaggcgcg
      241 catgcggtgg gtcggggtta cagggaaaat gaagaacgcg tgcgtgcgta ccgcacaccc
      301 tacatgcggg ctacggcttg ctaggattgc tcgttatact taattttgac aaggagtcaa
      361 tgaaatggcg atagaactaa atggtaactg gacaaaagga tttgctttag accatcatat
      421 gcaaaaaagt attttgttag gttatgatga attagggcat cctcactttg atagcaagag
      481 aagtgtgatt ggagaatggg tttatcaatt aaaatatcgt aatcaaaccc aaaatgtttc
      541 tttgctggta gactatattt tacaaaaatt tagtggttta gaatctataa atttaattgt
      601 tcccgcccct tttacaacag agcgtattaa tcaaccagtt caattaattg ccaaagaact
      661 aagtgatcgc ttgaatattc catattctcc tattttaaga aaatctagct cacacacacc
      721 actcaaaaat attgaagaaa aatcagaaaa actagaaata ctgaataatt ccatcacaat
      781 tgacaatata gatctatcca ataagaatat tttagtaatc gatgatttat ttgatagcgg
      841 agcaacattg gaaatatcaa ctgaaaaatt gttttcaaaa aatgctagat ctgtaattgt
      901 tttagcaatg acaaagacta gaggataaaa atgcgcacta ttttcttttc tggttcacgc
      961 agcatttcac gactaaatcc acaaatacga gaaagaatta acaatattct tagcaataat
     1021 tttgatattg ttattggaga tgctaatggg gctgataaag ctatccaaga atttctacaa
     1081 gaacaagatt atgctaatgt gcatatttac ttttctggca aaatttaccg aaataatgtg
     1141 ggaaattggc agtttgtcca agtagacagc aaaggaactg gaagggtatt ttatacagca
     1201 aaagataaga aaatggcaga aattgctgat tatggcttta ttttatggga tggtaagagt
     1261 attggttcat taaataatat agcagaactt ttacagctaa ataaaccttc tcttgtatac
     1321 cattctcaaa caaaagaatt ctttaaaatt aaatcaagcg cagatttaga gaatatttta
     1381 tctaacatag aagatgatgt tttagcttca attttagaaa agggaaatac ttttcttaaa
     1441 agctatgtaa caaaacaacc ttctttaatt caagagtaac cacccatgcc caaacgtacc
     1501 gacctaaaat ccatccttat catcggcgcc ggccctatcg ttatcggtca ggcctgcgaa
     1561 tttgactatt cgggcgcaca ggcctgcaaa gccttgcgtg aagaaggcta taaagtcatt
     1621 ttggtgaatt ccaaccccgc cacgattatg accgaccccg aaatggcgga tgttacctac
     1681 atcgagccga ttatgtggca gacggtggag aagattatcg ccaaggagcg gcccgatgcg
     1741 attctgccca cgatgggcgg tcagaccgcg ctgaactgtg cgctggattt ggcgcgtaac
     1801 ggcgtgctgg cgaaatacaa tgtcgagtta atcggcgcaa cggaagacgc gatcgacaag
     1861 gcggaagacc gcggccgctt taaagaagcg atggaaaaaa tcggcctctc ttgcccgaaa
     1921 tcttttgtct gccacaccat gaacgaagcc ttggcggcgc aagaacaggt cggctttccg
     1981 acgctgattc gtccgtcttt cacgatgggc ggttcgggcg gcggcattgc ctacaataag
     2041 gatgagtttt tggcgatttg cgaacgcggt ttcgatgcgt cgcctacgca tgagctgctg
     2101 attgagcagt ctgtgctcgg ctggaaagag tacgagatgg aagtggtgcg cgataaggcg
     2161 gacaactgca tcatcatctg ttcgattgaa aacttcgacc cgatgggcgt tcatacgggc
     2221 gactcgatta cggttgcgcc ggcgcaaacg ctgacggaca aggaatacca aatcatgcgc
     2281 aacgcttcgt tggcggtatt gcgcgaaatc ggcgtggaca cgggcggctc gaacgtgcag
     2341 tttgcggtga accctgaaaa cggcgagatg attgtgatcg agatgaaccc gcgcgtgagc
     2401 cgttcgtccg cgctggcttc caaagcaacg ggcttcccga ttgcgaaggt ggcggcgaag
     2461 ctggcggtcg gctttacgct ggacgagttg cgcaacgaca tcaccggcgg ccgcacgccc
     2521 gcgtcgttcg agccttccat cgactatgtg gtaaccaaaa tcccgcgttt cgcgtttgaa
     2581 aaattccccg ccgcagacga ccgcctgacc acgcagatga aatctgtggg cgaagtaatg
     2641 gcgatgggcc gtaccattca ggaaagcttc caaaaagcct tgcgcggttt ggaaaccggc
     2701 ttgtgcggtt tcaatccgcg cagcgaagac aaagcggaaa tccgccgcga actggccaac
     2761 cccggccccg aacgtatgct gtttgtggca gacgcgttcc gcgcgggctt cacgccggaa
     2821 gaaatccacg agatctgcgc catcgaccct tggttcttgg cgcaaatcga agacttgatg
     2881 aaggaagaaa aatcggtttc agacggccag ttgcaagatt tggattacgc cgccctacgc
     2941 cgtctgaaac gcaaaggctt ctccgacaaa cgtttggcac aattgttgaa cgtaagcgaa
     3001 aaagaagttc gcgaacaccg ctacgcgctg aagctgcatc cggtttacaa acgcgtcgat
     3061 acctgcgccg ccgagttcgc caccgaaacc gcctatcttt actccactta cgaagaagaa
     3121 tgcgaatccc gtccttccga ccgtaaaaaa gtgatgattc tcggcggcgg cccgaaccgc
     3181 atcggtcagg gcatcgagtt tgactactgc tgcgttcacg ccgcactcgc cctgcgcgaa
     3241 tcgggctttg aaacgattat ggtcaactgc aaccccgaaa ccgtgtccac cgacttcgac
     3301 accagcgacc gcctctattt cgagccgctg acattggaag acgtgttgga aatcgtccgc
     3361 accgaaaacc cgtggggcgt gattgttcat tacggcggtc aaaccccgct gaaactcgcc
     3421 aacgcattgg ttgaaaacgg cgtgaacatc atcggcacat ccgccgacag catcgatgcc
     3481 gccgaagacc gcgaacgctt ccaaaaagtg ttgaacgact taggcctgcg ccaaccgccc
     3541 aaccgcatcg cccacaacga agaagaagca ctcgtcaaag ccgaagaaat cggttatccg
     3601 ctggtcgtgc gcccgtctta tgttttgggc ggacgcgcga tgcagattgt ccactccgcc
     3661 gaagccttgc aaaaatacat gcgcgaagcc gtgcaggttt ccgaagacag ccccgtgctg
     3721 ctcgatttct tcctcaacaa cgcgattgaa gtcgatgtgg actgcgtttc agacggcaaa
     3781 gatgtggtta tcggcggcat catgcagcac gtcgaacagg cgggcatcca ctccggcgac
     3841 tccggctgct cgctgccgcc atactcctta agcgaagaaa tccaagacga aatccgccgc
     3901 caaaccaaag cgatggcgta cgcgctgggc gtggtcggac tgatgaacgt gcagtttgcc
     3961 gtgcaggacg gcgtggtgtt cgtgttggaa gtgaacccgc gcgccagccg taccgtcccc
     4021 tttgtctcca aagccaccgg cgtgccgctc gccaaagtcg gcgcgcgctg catggcaggc
     4081 atttccctga aagaacaagg cgtggaaaaa gaagtcgtcc ccgatttcta tgccgttaaa
     4141 gaagccgtgt tcccattcat caaattcccg ggcgtggata cgattttgag tccggaaatg
     4201 cgctccaccg gcgaagtgat gggcgtgggc gcaagtttcg gcgaagccta ctacaaagcc
     4261 caactcggcg cgggcgaacg cctcaacccg accggcaaaa tcttcctctc cgtgcgcgaa
     4321 gaagacaaag aacgcgtcat taaaaccgcc aaaaacttcc aagccttagg ctacggcatc
     4381 tgcgccacgc gcggcacggc gcaatacctg accgaacacg ggctgattgt gcaggccatc
     4441 aacaaagtcc ccgaaggccg cccgcacatc ggcgacgcgc tgaaaaacgg cgaaatcgca
     4501 ctggtcgtaa ataccgtttc cagcgatccg caatccgtgt ccgacagcca catcatccgc
     4561 caaagcgcat tgcagcaacg cgtgccgcaa tacaccacca ccgccggcgg cgaagcgatg
     4621 agcgaaggcg cgaaaagccg ggattatctg ggtgtgtaca gcgttcaaga gctgcacggt
     4681 cgtttgaaaa accgcaactg atgcctgaat caggttgaac aatgccgtct gaagccgttt
     4741 tgcggtttca gacggcattt tgttgcacat agccttcact ttcttttgac ctttaagcac
     4801 cctaatttgg ggcggtggat tatccaactg ccggacatcc ataaaaatac cgcccgaacc
     4861 acggttcaga cggcatctgc cttgcaacgg acagcagcag tttaaagccc gcccccttcg
     4921 ttccgcccca aaaccatcgc atctccgtag ctgaagaaac ggtattcacg ttcaatcgca
     4981 tgacggtaca cggcgcggat atgacccata cccgaaaacg cgccgaccaa catcagcagc
     5041 gtcgatttcg gcagatgaaa attggtaacc agcctgtcga caacattaaa acggtagccc
     5101 ggcgtgatga aaatatcggt gtcgccctgt ccgtctttca aatatcccgt tgcgcgcgcg
     5161 gcagactcga gggcgcgcat ggaagtcgtg ccgaccgccc aggctttgtt cccccgggct
     5221 tttgccgcct caacggcggc gacggtttca gacggcactt caaaccattc gctgtgcatt
     5281 ttgtgttctt cgattttatc gacgcgcacg ggttggaatg tccccgcacc gacgtgcagg
     5341 gttacttctg cggttaccgc gcctttgtct ttcagacggc gcaaaagttc ttccgtaaaa
     5401 tgcaggcccg ccgtcggcgc ggcgaccgcg ccctgatatt tggcataaac agtttgataa
     5461 cggctgtcgt cgtccgcatc ggcggcacgt tcgatataag gcggcagggg caggtgtccg
     5521 ttctgttcca aaagttcgta aacggtttga ccgccttcaa aacgcaggca gaacagttcg
     5581 ccctcacgtc cgaccatcac ggcacggata ccgccttcaa acaccagccc cataccgggc
     5641 ttgggggact tggacgaacg gatgtgcgcc aatgcggtat ggttgtccaa cacacgctca
     5701 atcagggctt cgatcctgcc gccgctgtct ttctgcccaa acagccgcgc cttcatgact
     5761 ttggtgttgt tgaataccaa aacgtcgcct gcctcgacat aatcgggcaa atcgccaaac
     5821 acccggtctt gcagcggcat atcgggcagc gcgaccaaaa gccggctgct gccgcgcacc
     5881 tcgggcggat gctgggcaat caggtgttcg ggcagggtaa agtcaaaatc tgaaatatcc
     5941 atttttacac tctcgttcgg gcaagccgcc attatacgca ctttagccct ttttcagacg
     6001 gcatctttgc ccgaaaaacc aacagattag aataaacact cttgactcgg ggcatcttgt
     6061 gcgcaaaatc aaacttcctg cacatttccc ccaaaaaccg ccgttttttg atattttact
     6121 ggacatttac cgacaacttc gggaaaatga acacaatttc acggtcgttt cccaccacag
     6181 gaaaaccgta tccgaacacc attccgcccg gtttgcgccg ttgccgcaag ccggctgttt
     6241 tctgaaaaac caacgcaaca acccgccgga acaccggcag cctttaaagg aacagaaatg
     6301 gatttgcgca aattaaaaaa actgattgat ttggttgaag aatcgggtat cgccgaaatc
     6361 gaagtaaccg aaggcgagga aaaagtccgc attacccgta ccattgcagc tgcggcagct
     6421 cccgtttacg ccgcgcccgt acctgccgcc gcgccggccg taacgccggc cgccgcaccc
     6481 gttgcggcat ccgcgcccgc cgccgcgccg gccgcccgcg atttgtccga cgctcaaaaa
     6541 tcgcctatgg tcggcacgtt ctaccgcgcg cccggcccga atgccgccgc atttgtcgaa
     6601 gtcggtcaac aggtcaaagc cggcgacacg ctgtgcatca tcgaagcgat gaagctgatg
     6661 aacgaaatcg aagccgaaaa atccggcacg gtcaaagaaa ttctggtcga aaacggtacg
     6721 cccgtcgaat tcggcgaacc gctcttcatc atcggataat cctgttttca gacggcataa
     6781 acttccgatg ccgtctgaaa tgctttcccc cccttcagcg ttcccgcacc cttttttacg
     6841 gacgggttgc cggaaccgca ggaaaggtca tcatgctgaa aaaagtttta atcgccaacc
     6901 ggggcgaaat cgccttgcgc gtactccgtg cctgccgcga aatgggcatt gccaccgttg
     6961 ccgtgcattc cgaagccgac aaaggcagcc tgcacgtcaa actcgccgac gaatccgtgt
     7021 gcatcggtcc tgccgcttct gcgcaaagtt atctgaacat tcccgccatc attgccgccg
     7081 ccgaagtaac cttcgcggac gctgtccacc cgggctacgg cttccttgcc gaaaacgccg
     7141 atttcgccga acaggtcgaa cagtccggct ttaccttcat cggcccgaaa cccgacacca
     7201 tccgcctgat gggcgacaaa gtctccgcca aacacgcgat gatagcggca ggcgtgccct
     7261 gcgtccccgg ctctgacggc gcattgcccg acgatgatgc cgaaatcctc aaaatcgccg
     7321 acaaagtcgg ttatcccgtg attatcaaag catcgggcgg cggcggcggg cgcggtatgc
     7381 gcgtggtcga gaaaaaagaa gacctcctcc agtctgtcga aatgaccaag gctgaagcgg
     7441 gcgcggcgtt cggcaacccg atggtttata tggaacgcta cctgcaacgc ccgcgccacg
     7501 tcgaaatcca agtgcttgcc gacgaacacg gcaacgccgt ctatcttgcc gagcgcgact
     7561 gttcgctgca acgccgccac cagaaagtca tcgaggaagc acccgccccg tttatcgatg
     7621 aagaggcacg caaaaaaatc ggcaaagcct gtaccgacgc gtgcaaacgc atcggctacc
     7681 ggggcgcggg cacgtttgaa tttttatacg aagacggcga atttttcttt atcgagatga
     7741 acacgcgcgt tcaggtcgag catccggtta ccgaactcat taccggcgtg gacatcgtcc
     7801 aagagcaact ccgcatcgca tccggcctgc cgctgcaata caaacagaaa gacatcaaaa
     7861 tcgaaggcca cgccttcgag tgccgcatca atgccgaaga cccgtacaac ttcatcccca
     7921 gcccgggccc gattgaaagc tgccacctgc ccggcggctt cggcatccgc gtggacagcc
     7981 acatctacca aggctaccgc atcccgccgt actacgacag cctgatcggc aaaatctgcg
     8041 tacacggcaa aacgcgtgaa caggcaatgg cgaaaatgcg cgtcgcactc gccgagctgg
     8101 cggtaaccgg catcaaaacc aatacgccgt tgcaccgcga cctgttcgcc gatgcgggtt
     8161 tccaagaagg cggagtgagc atccattacc tggagcactg gctggaagcg cgcaaaacca
     8221 aacaggacaa ataagccgcc gccgatatgc cgtctgaagc cgcccttccg cgttcagacg
     8281 gcatttccct tgccccgccg tctgaaaccg atttcgatat ttccaagaaa gcccgttatg
     8341 ccctaccaac aaatcaccgt caacgtcaac gatgccgtcg ccgaacgcct cgccgacgtg
     8401 ctgatggaac acggtgcact ctccgccgcc atcgaagatg cctgcgccgg cacgcaaaac
     8461 gaacaggcga ttttcggcga acccggtatg cccaccgaac aaatctggca gcagagcaaa
     8521 gtcatcgccc tgttcggcga acacgacgaa gccgccgccg tcatcgatgc cgccgcacaa
     8581 gaatgcgggt taaaagactt ggcatacacc ggcgaaacca tcgaagacca agactgggtg
     8641 cgcctcacgc aatcgcaatt cgaccccatc cgaatttccg accgcctgtg gattaccccc
     8701 tcttggcacg aagcccccga aggctgcgcc gtcaacctcc gcctcgatcc cggactcgcc
     8761 ttcggcaccg gcagccaccc gaccacgcgc ctctgcctca aatggctgga tacgcaactc
     8821 aaaaacggcg aaagcgtcct cgactacggc tgcggttcgg gcatcctgac catcgccgcc
     8881 ctcaaactcg gtgcaggttc cgccgtcggc gtggatattg acgaacaggc cgtccgcgcc
     8941 ggcagggaca acgccgaaca gaacaacgtc gatgcacaat tcttcctgcc cgacagtctg
     9001 cctcaagggc aattcgatgt cgtcgtcgcc aacatcctcg ccaacccttt gcgtatgctc
     9061 ggcgaaatgc ttgcagcccg caccaaacag ggcggacgca tcgtgttgtc cggtttgttg
     9121 gatgaacagg ccgaagaact cggcggcatt tacagccaat ggttcgatct cgacccggcg
     9181 gaaactgacg aaggctgggc gcgcttgagc ggcgtaaaac gctgaaacgg aaaggaaaca
     9241 ccgtgcagga caaaaacaac ctctgctggc tcgatatgga aatgacgggg ctgaatcccg
     9301 aaaccgaccg cattatcgaa gtcgcgatga ttattaccga ctcggatttg aatgtgttgg
     9361 cgcaatccga agtttacgcc atccaccaaa gcgacgatct gctcgacaat atggacgagt
     9421 ggaacaccgc cacacacggc aggacggggc tgacgcagcg cgtgcgcgaa tcgtcgcata
     9481 ccgaagccga agtcgaacag aaactgctgg actttatgtc ggaatggata cccggacgcg
     9541 ccacaccgat gtgcggcaac tccatccacc aagaccggcg ttttatggtc aaatatatgc
     9601 cgaaactgga aaactacttc cactaccgca acctcgacgt ttccacgctg aaagaactcg
     9661 ccaaacgctg gaatccgccc attgccaaaa gcgtcgtcaa acgcggttcg cacaaggcat
     9721 tggacgacat tttggagagc atcgaagaaa tgcgccacta ccgcgaacac ttcctgattt
     9781 ccgccccgaa agccgaagcg caataagaaa caaacaatgc cgtctgaaac gct
//
LOCUS       DBJGGI010000069         9622 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.069, whole genome shotgun sequence.
ACCESSION   DBJGGI010000069 DBJGGI010000000
VERSION     DBJGGI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9622
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.069"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(63..938)
                     /locus_tag="ACSIGJ_001751"
     CDS             complement(63..938)
                     /locus_tag="ACSIGJ_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HHQ1330568.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     SVLKPDVGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKSDSGKKSGSAWRQALLLLGVWAGYGI
                     IDILFKQIAKSGTAFAGNLLVAFALAGVLMFACLFAKSVRWRVESVVGGILLGGLNFM
                     NIVTYITAHQMMKDSPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLSLCSI
                     ACLFYWTEVKALFGI"
     gene            complement(942..1634)
                     /locus_tag="ACSIGJ_001752"
     CDS             complement(942..1634)
                     /locus_tag="ACSIGJ_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HHQ1330569.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPQAEE
                     KFKEIQRAYDTLSDLSKRTQYDASFRRHEERGRQEEAFRREQARREQFYGEQMRREQA
                     FRREFERQASRSRHAYEPSGSGSGRNYILAAYILFGLGAIMQFMPIVGVILAYMKRNS
                     LDSIVYAAHTEYLIKTFWRTFWLYLLGALTARFGIGVLVIIATTVWYFYRIIAGFIRF
                     NDGRAVAPEKWI"
     gene            complement(1656..2135)
                     /gene="ybaK"
                     /locus_tag="ACSIGJ_001753"
     CDS             complement(1656..2135)
                     /gene="ybaK"
                     /locus_tag="ACSIGJ_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HHQ1330570.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGPIVLMHGDKQISTRNLARHLGAKHIEPAAPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPDDLNILNPKTIQAAV"
     gene            2210..2572
                     /locus_tag="ACSIGJ_001754"
     CDS             2210..2572
                     /locus_tag="ACSIGJ_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HHQ1330571.1"
                     /translation="MDIRYFGTTPRYSEAVCAGGLIFLSGMVPENGETAAEQTADVLA
                     QTDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            2607..3470
                     /locus_tag="ACSIGJ_001755"
     CDS             2607..3470
                     /locus_tag="ACSIGJ_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005739 - mitochondrion [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgfZ/GcvT domain-containing protein"
                     /protein_id="HHQ1330572.1"
                     /translation="MKTLLPFFGVARVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGDDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     AASAEPLAAQEPSLAFTSECVSDGICSVILHHRGILHIAPETALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAAEAGILLTADGEEAGIVLDSVQDSENFTALAVIKFSAAQKELTVPNGGIFKA
                     VHLFFKTKNAK"
     gene            3562..4521
                     /gene="ttcA"
                     /locus_tag="ACSIGJ_001756"
     CDS             3562..4521
                     /gene="ttcA"
                     /locus_tag="ACSIGJ_001756"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HHQ1330573.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDTINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRRLQASAPIDFELVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATMFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQNLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
     gene            complement(4615..7554)
                     /locus_tag="ACSIGJ_001757"
     CDS             complement(4615..7554)
                     /locus_tag="ACSIGJ_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HHQ1330574.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFM
                     IVDEPHKFPTRDSAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFAE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGICTAVNEAYINNGLMLSRRIDSIEDEDFIR
                     FNTMTYREFLEKLAQTAKIQMQTLHQAFYRVRDELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADLGRFEDTEHRPAAGYLFGEIF
                     YDSDIEHENVANNQIEGVIVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENEH"
     gene            complement(7644..>9622)
                     /locus_tag="ACSIGJ_001758"
     CDS             complement(7644..>9622)
                     /locus_tag="ACSIGJ_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HHQ1330575.1"
                     /translation="TAGANTEAIYTSDGITANSTQLEQLKKLFPACFDADGNFLIDRL
                     QAEIAPQTDIGREFYEMNWLGKSYARLLRNLPPETLISEDKTHNAKPENAGSQNLLIR
                     GDNLEVLKHLKNAYANSVKMIYIDPPYNTGSDGFVYQDDRKFTPAELALLANIDEDEA
                     ARILDFTDKGSNSHSAWLTFMYPRLYIARELLKDDGVIFISIDDNEAAQLKLLCDEVF
                     GEGNFVAQLPWRKRTAKSDVPFGISQDYEWIFVFAKSCQFIAATKGKERRYYETDDFP
                     DRPWRTHDLTKQTTAAERPNSFFTMVDPKTGKKYPANPNATWRVTKDTFQDYYNKGKI
                     VFPDDYDFLNISNPVMRYFKDDDMKKAGEDFGKVAVSSRLPENVGTLADAVAEYLAIF
                     SRTLPENIGMTKEGTKEITDLFGSKIFTFPKPSQLIKFLVSISSKSNDLILDFFAGSG
                     TTAHAVMQLNAEGQNGNRRYICVQLPEKTAEKSEARKAGYPTIFDITKARIEKAAAKI
                     RVEHPDYTGDSGFKIFQTADNFRQHPDKDFSPEQPDLPLNDELSEEQLQTLLTTWTLY
                     DGAALTTPVEPVRLGSYTAYLCEKRLYLLNKGFTSADLLAFIKKLDDDADFNPNRVIV
                     FGSNMASAMQHELDQAVRGYTNKKEIELNVIIRV"
BASE COUNT         2248 a         2545 c         2311 g         2518 t
ORIGIN      
        1 aatgccgtct gaagcagcat tttcctgttt cagacggcat tttgttatat caatcagccg
       61 gattatatgc cgaacagtgc cttaacttct gtccaataga acaggcaggc aatggagcat
      121 aatgacagta caatgcctgc cgtattgatt gtgttgattt tttccttaaa gaacaatgcg
      181 cccgaaagcg tccctaaaac aatcacaccg atattcatac cggcaaaaac cagcgtggga
      241 ctgtccttca tcatttggtg cgcagtgatg taggttacga tgttcataaa attcaaaccg
      301 cccaagagta tgccgccgac cacgctctca acgcgccatc tgaccgattt ggcaaacagg
      361 caggcaaaca tcagcacacc cgccagcgca aatgcaacca gcaggttgcc cgcaaatgcc
      421 gtgccgcttt tggcaatctg tttgaacagg atgtcgataa tgccgtaacc cgcccacaca
      481 ccgagcagca ataacgcctg ccgccacgcg ctgcctgatt ttttgccgct gtcgcttttc
      541 caaagcaggc agaacagtgc ggcaaaggcg aggcacagtc cgatcagttt gccttcactg
      601 agtttttcgc caaacagcgt caaagcggca acaatcggta aaaacagcga caaacgctgc
      661 gccgcgtcgg atttgacgat accggcggat tctacggatt tgcccataat gacaaatacg
      721 gacggcagca gcacgcccaa agcggcaaac agcggccacg tcggcaaaaa tgcaccgata
      781 ttgccgacat ccggcttcaa taccgacagg gtcagtatga ccgcgaccac ataattgacg
      841 gcgaccgcct gcgcgatgtc gatttttttc ttccttgcca ttttcagcaa aatggaaacc
      901 gacacgctga acacgatgct gattaacagg taggccatgc tctatatcca tttctcgggc
      961 gcaaccgccc tgccgtcgtt gaagcggata aagccggcga tgatgcggta gaaataccag
     1021 acggtcgttg caataataac cagcacgccg atgccgaaac gggcagtcag cgcgcccaaa
     1081 agataaagcc aaaatgtgcg ccaaaaggtt ttaatcaggt attcggtatg tgcggcatag
     1141 acaatgctgt ccaaactgtt ccgtttcata taggcgagaa tcacgccgac tatgggcatg
     1201 aactgcatga ttgcacccaa accgaacagg atgtaggcgg cgaggatata gttgcgcccg
     1261 cttccgctgc cggacggttc gtaagcatgg cgcgaacgtg aagcctgccg ttcaaactcc
     1321 cgtctgaacg cctgttcgcg gcgcatctgt tcgccgtaaa actgctccct gcgcgcctgt
     1381 tcgcggcgga atgcctcttc ctgccgcccg cgttcctcat gccgtctgaa ggacgcgtcg
     1441 tattgcgtcc gttttgacag gtcggaaagc gtatcgtaag cccgttggat ttctttgaac
     1501 ttttcttccg cctgcggatt gcccggattg cggtcgggat ggtatttcat cgccagcttg
     1561 cggtaggcgc gtttgatttc gtccgccccc gcctgcggcg acacgcccaa tacggcatac
     1621 aaatctttgt ccatattgct ccttatactt cccggtcaaa ccgctgcctg tattgttttc
     1681 gggttcaaaa tatttaaatc atcggggcgg ataccgataa tgaacccgcg ttttccgcca
     1741 ttgatataga tggtttccaa atccatcacc gactgttcga cgtaaatatc caattttgtc
     1801 cggatgccga acggcgttgt gccgccgacc agatagcccg tccacttgtt tgcctgcgca
     1861 ggcgcggcgg gttcgatgtg tttcgcaccc aaatgccgcg ccagattgcg ggttgaaatc
     1921 tgtttgtcgc cgtgcatcaa gacaatcggc cccttaccgt tttcatcttg caaaacaatg
     1981 gttttaatga ccaagtgttc gtctttgccg aacagtcggg caaactgcgc cgtgccgccg
     2041 tgttcctcat aggcataggt aaaaggttca aattcgatgc cgttttcacg caaaacgcgt
     2101 acggcaggtg ttatcggata agtgtgttta ctcatttttc gtcatgcaaa acatggaaat
     2161 cggtcaagcg caatgttacc atatatcccc tttatcgaag gaaaccgata tggatatccg
     2221 gtatttcggc acaacgcccc gctattccga agcggtttgc gcgggcggct tgattttcct
     2281 ctccggcatg gttcccgaaa acggtgaaac ggctgccgaa cagactgccg acgtgcttgc
     2341 ccaaaccgac cgctggctgg cggaatgcgg ctcggacaag gcacacgttt tggatgcggt
     2401 catctacctg cgtgatatgg gcgactatgc ggaaatgaac ggcgtgtggg acgcttgggt
     2461 tgccgccggc agaacgcccg cccgcgcctg cgtggaagcg cggctcgccc gtccggagtg
     2521 gcgcgtcgaa atcaaaataa ccgccgtgaa gagagacgcg gcaactgcat aaacagattt
     2581 ccttatattt tcagagtacg caaatgatga aaaccttact gccctttttc ggcgtggcgc
     2641 gcgtcagcgg cgaagaccgc caaaccttcc tgcacggaca gctttccaac gacatcaata
     2701 atcttcagac cgggcaggcg tgttacgcga cttacaacac gcccaaaggc cgcgtcatcg
     2761 ccaatatgat tgtcgtcaac cgtggagacg atttgctgct gattatggcg caagatttgc
     2821 ttgaagcaac ggtcaaacgc ctgcggatgt ttgtgttgcg cgccaaagcc gtttttgaaa
     2881 tccttgaaga ttacgccgtc ggcgcggaat tggcagcatc cgccgaaccg cttgccgcgc
     2941 aagaacccag tctcgccttt acctccgaat gcgtctcaga cggcatctgt tccgtcatcc
     3001 tgcaccatcg gggcattttg cacatcgccc ccgaaaccgc cctaccccct tatgacgctg
     3061 ccgccgaaaa cgcgtggcgg ctgcacgaaa tccgcagcgg ttatccgtgg atatgcgcgg
     3121 ctaccaaaga aaccgccgtc gcccaaatgc tcaaccagca catcatcggc ggcgtacact
     3181 tcaaaaaagg ctgctacccg ggacaggaaa tcatcgcgcg cgcccagtat cgggggcagg
     3241 tcaaacgcgg cttggcggta ttgtcgggaa attcggcagc cgaagcgggc atcctgctga
     3301 cggcggacgg cgaagaagcc ggcatcgtac tcgacagcgt tcaagattcg gaaaacttta
     3361 ccgcccttgc cgtgattaaa ttctccgccg cgcaaaaaga actcaccgtc ccaaacggcg
     3421 gtatcttcaa agcggttcat ctcttcttta aaaccaaaaa tgcaaaataa gccctctaat
     3481 gccgtctgaa gcccttcaga cggcattgcc gcccctgttt tcggttacaa tgccgcatcc
     3541 ctaatttttc aaaccaccgc catgtccaaa aaaaccaagc aagaactcga aaacaacaaa
     3601 ctcagcaaac gcctgcgcca cgccgtcggc gacacgatta acgatttcaa catgatcgag
     3661 ccgggcgaca aaatcatggt ctgcctctcc ggcggcaaag acagctacgc cctgttagac
     3721 atcctgcgcc ggctccaagc cagcgcgccg attgattttg aactggtcgc cgtcaatctc
     3781 gaccaaaaac agccgggctt ccccgaagaa gtattgccga cctatctcga aagcatcggc
     3841 gttccctaca aaatcgttga agaagacact tactccaccg tcaaacgcgt gttggacgaa
     3901 ggcaaaacga cttgttcgct gtgcagccgc ctgcggcgcg gcatcctcta ccgcaccgca
     3961 aaagaattgg gctgcaccaa aatcgccttg ggacaccacc gcgacgacat cctcgccacc
     4021 atgtttttaa atatgtttta cggcggcaaa ctcaaagcca tgccgcccaa gctggtgagc
     4081 gacaacggcg aacacatcgt catccgcccg ctggcgtatg tgaaagaaaa agacttaatc
     4141 aaatacgccg aattgaaaca gttcccgatt atcccgtgca acctctgcgg ctcgcagccc
     4201 aacctacaac gccaagtcat cggcgatatg ctgcgcgatt gggacaaacg cttccccggc
     4261 cgtatcgaat cgatgttctc cgccctgcaa aacgtcgtcc cttcccacct tgccgatacc
     4321 gaactctttg actttgccgg attggaacgc ggtcaaaatc tgaaacacgg cggcgatttg
     4381 gcttttgaca gcgaaaaaat gcccgaacgc ttttcagacg gcagcgaaga agatgagagc
     4441 gaaatcaaaa tcgagccgca aaaagccgaa cgcaaagtca tcaacattct ggcgaacaag
     4501 ccgaaaacct gcggcgcgta aacctccacc gtcaaaaatg ccgtctgaag cccgtttggc
     4561 gtttcagacg gcattttaaa atcccgaagc agacggcttc caaacatttg tttctcagtg
     4621 ttcgttttca gaatgcccgc ctgctggcat attttgcccg atcagttcgg ctatcctctc
     4681 gccgtcaaac tgcgttttga acaccacctt gatttctttg gaaatctcgg caaacagctt
     4741 ttcggcatgt ttgattttcc gctcttcgct ttttcgcaaa tcttccgccc cgtcagtccc
     4801 tttggcttca atcacaaagt tcagaatctc gccgcttttg gttttcacga tataggcaaa
     4861 gtcgggcgaa tacgtgccgc cgccggcaac agggattttg atggagtttc tcggtatttt
     4921 ggtaaatacg attacgcctt caatttggtt gttggcgaca ttttcatgtt ctatatccga
     4981 atcgtagaaa atctcgccga agagatagcc ggcggcaggc cggtgctccg tatcttcaaa
     5041 tctgcccaaa tctgcttttt tcaccgcgcg cggtttgccg tctttattgg taaatttggt
     5101 cggatggatt ttgctgccga caagccggta atccagttcg aatttatgga aggaatgatg
     5161 aagcaaaaac cggttgaagc cgtttttgat ttgggcgatg gtctgcatat tcaaaaaatc
     5221 gccaatgttc agttcgtcgc ggacgcggta aaacgcctga tgcaaagtct gcatctggat
     5281 ttttgccgtt tgtgccagtt tttccagaaa ctcacggtaa gtcattgtgt tgaaacggat
     5341 aaaatcttca tcttcaatac tgtctatgcg gcgggaaagc ataagcccgt tgttgatata
     5401 agcttcgttt accgccgtgc atatgcctgc ctgcgggaat ttggcggcgt tttcacgcaa
     5461 ataggcggta aataaatcga caaattcggc ttcatctttg attttgtact gcaaaacggc
     5521 tttatggtga atcagctccc acaaggcttt gagttcttca tatttgcctt cgcgcatgat
     5581 gatggtgtct ttgccctcgt ctttggcgtt gctgactttg cctttgtcca aacctttggg
     5641 gaaggcttcg ggataggcgg cttttaattt gtcatagccg tcttcggcaa agttttcatt
     5701 gtcgtcaatg atgccgtctg aaaacagttg gtttaccaat accagcggtt tgatatcggg
     5761 gtatttttgc aatatttttt gtttcagctc ttcggtaaac tttttggaga tttcttcctg
     5821 aaaagaattg tcgttgattt cgccgacaag ctgcttcaca aagtcttttt cgctgctatc
     5881 gacaaaataa ttcagtttgt acggtacatc gcgcacccgc gccatcagct cgtttaccgg
     5941 caggcgcagg ccgcgtccga cttcttgcag cttggaagtc gtgctgccgc tggaacgcag
     6001 tttgcaaatc tggaaaacgt tgggattgtc ccagccttcg cgcagcgtcc atttggaaaa
     6061 aataaagcgg cgcgggttgt ccaaagacag cagtttttcc ttatcgtgca ggatttcatt
     6121 gatttcctgc tcgattctat cgtcgctgtc tgtattgtct ttggaaaaat agccaccgtg
     6181 gcaggcggat acatcgtcca acgtcttttg caggtaatcg cggtaaaacg ggtcgctttc
     6241 cgttttcaga cggcgtgccg cttccgcgcg aatccagctt tcaaatttat ctttcaggct
     6301 gcctgaaagc tcgttgccgc tgcggtagcc cgcgatatcg tcaataaaaa acagcgtcag
     6361 cggcttgatt ttgggctgtg gcgcgcgttc tgccaaaagc gcgcgttcca gcttgaaatg
     6421 ttcggcaacc gcccgctgca tcatcgcatc ctgcaccgtt tgcgaatagg aataagggtt
     6481 gatgacggca cccgttttca actccaagcc gttgcttaac accaccacgg ttttattcat
     6541 tttgtcgatt ttcaaatccg aaatagccgg atggatttgc gccaaatctt cgccttttgc
     6601 cagtttgaac gtctgctttt tgtccttttc gtttaattca aatttcgctt ctttgccgtc
     6661 cgacgacacc agttttaccg ccgcatccat gccgccctgc atttcttcct gaaacacgcg
     6721 cacgcctttg accagcccgt cgttaaacgc gtctactgcc gtcaaacggt aaagcaagtt
     6781 gtaatattca tcgttaaatg ttgcaccgta gcgcaaaata tattgcggtt ttaagcgttt
     6841 gatattgccc cacgttttcg cgctatctcg ggtcgggaat ttatgcggtt cgtccacaat
     6901 cataaacggg cgcacggcag ccaatgcatc aacgggattg tcaaacaaat ccttcaatgc
     6961 cttgtcgccc gtatcgttca tggacgacga attaaccatg cccgcgttaa tcagcagcac
     7021 atgaatttcc tttttgtttt ccgctttgac aaattgctca atcgttatgg gcgcattgga
     7081 ctttttgccc ttattctttt tcgcgctttc caccacatag gttttcaggc gtacgccttc
     7141 ataatcgccg ccgaaatcct gttcaaaatg ctctgccaaa gccttgcttt gcaaaaactg
     7201 ctgtgttccc gccttaatgg acaaagtcgg cacgaccacg ataaatttga acacgcccag
     7261 ccaacggtgc agctcgaaca tggtttgtgt gtaggtatag gttttgcccg tgcccgtttc
     7321 catggaaata tcaaggatat tttggtcgtc cgaacggtcg gggaatcggc cgtctatacc
     7381 gttttggctt tgcactttca ggatattgtt tgcgtattgt tttgcagcaa acaaaagttc
     7441 gggattttcg tctgccgtcc gatatttggg cgttgccccg tcaaacacgc ccaaaaccgc
     7501 cgaaaccgcc cgcatttggt gcggctggtt tttctcgtaa ttaaaaccgc tcatgaatgt
     7561 ttctccatga agtcattgtt gtatcgtcct tcccccgcaa ggggaatcca agccgaaaag
     7621 cagcctgcac tttcggattc cggttacacc ctgataatca cattcaactc aatctctttt
     7681 ttattggtat aaccgcgaac cgcctggtcg agttcgtgct gcatggcgct tgccatattg
     7741 ctgccgaata caatcacacg gttgggattg aaatccgcat cgtcgtccag cttcttgata
     7801 aacgccagca aatcggcgga agtaaaaccc ttgttaagca gatacagccg tttttcgcac
     7861 agatacgccg tataactgcc caaccgcaca ggctcaaccg gcgtggtcag tgccgccccg
     7921 tcatacagcg tccaggtggt cagaagcgtt tgcagctgtt cttcgcttaa ttcatcgtta
     7981 agcggcaaat ccggttgttc gggcgaaaaa tccttgtctg gatgctgcct gaaattgtct
     8041 gccgtttgaa agattttgaa gcccgaatcg cccgtgtaat cgggatgttc gacgcggatt
     8101 ttggcggcgg ctttttctat gcgggctttg gtgatgtcga agatggtcgg gtagcctgct
     8161 ttacgggctt cggatttttc agcggttttt tcgggaagct gtacacagat atagcggcgg
     8221 ttaccgtttt gtccttcggc gttaagctgc atcacggcgt gggcggttgt gccgctgcct
     8281 gcgaagaagt ctaggattag gtcattactc tttgaactta ttgaaactaa aaatttaatc
     8341 aattgactag gcttggggaa ggtaaatatt ttgctaccaa ataaatctgt gatttctttt
     8401 gtgccttctt tagtcattcc gatattttca ggtagcgtcc tactaaaaat agccaaatat
     8461 tcggctactg catcggctaa tgtaccaaca ttttcaggta atctactgct tacagctact
     8521 ttgccaaaat cctcgccagc ttttttcatg tcgtcatctt taaagtaacg cataactgga
     8581 ttgctgatat ttaagaaatc ataatcatca gggaaaacga tttttccttt attataataa
     8641 tcttgaaatg tatctttggt tacacgccaa gttgcatttg gatttgctgg atattttttt
     8701 cctgtcttgg gatcaaccat tgtgaaaaaa ctatttggcc tttccgccgc agttgtttgt
     8761 ttcgttaagt cgtgggtacg ccaaggacga tcggggaaat catcagtctc ataatagcgt
     8821 cgttccttgc ctttagttgc tgcaataaat tggcaagatt ttgcgaatac aaatatccat
     8881 tcataatcct gcgaaatacc aaaaggcaca tctgatttag ctgttctttt tcgccaaggc
     8941 aattgtgcaa caaaattccc ttccccaaac acttcatcac acaacaattt caactgcgcc
     9001 gcttcgttat cgtcaatcga gataaaaatc acgccgtcgt cctttaacag ttcgcgggcg
     9061 atatacaggc gcggatacat aaaggtcagc cacgcgctgt gcgagtttga gcctttgtcg
     9121 gtgaaatcta aaatccgcgc ggcttcgtct tcgtcaatat tcgccaggag ggcaagttcg
     9181 gcgggtgtga atttgcggtc gtcctgatag acaaagccgt ccgatccggt gttgtagggc
     9241 gggtcgatgt aaatcatctt cacgctgttt gcgtaggcgt tttttaagtg tttcaacact
     9301 tccagattat cgccgcgaat cagcagattt tggctgcctg cattttcggg cttggcgttg
     9361 tgcgttttgt cttcacttat cagggtttcg ggcggcaggt tgcgaagcag gcgggcatat
     9421 gatttgccca gccagttcat ttcgtaaaat tcgcgtccga tgtcggtctg tggcgcaatt
     9481 tcggcttgta atctgtcgat aaggaaattt ccgtctgcgt caaaacaggc gggaaacagt
     9541 tttttgagct gttcgagttg ggtagagttg gcggtaatgc cgtctgaagt gtagattgcc
     9601 tcagtgttcg ccccggctgt gt
//
LOCUS       DBJGGI010000070         9362 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.070, whole genome shotgun sequence.
ACCESSION   DBJGGI010000070 DBJGGI010000000
VERSION     DBJGGI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9362
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.070"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(35..163)
                     /locus_tag="ACSIGJ_001759"
     CDS             complement(35..163)
                     /locus_tag="ACSIGJ_001759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330576.1"
                     /translation="MAKRKYKDKNSLHYTGANAVWKISGLAFSVLKYVNLVFYAEN"
     gene            162..1463
                     /gene="aroA"
                     /locus_tag="ACSIGJ_001760"
     CDS             162..1463
                     /gene="aroA"
                     /locus_tag="ACSIGJ_001760"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HHQ1330577.1"
                     /translation="MTESVRLPAASLKPSTVALPGSKSISNRTLLLAALSDNVCEIHS
                     LLKSDDTDRMLEALDKLGVQIEHLAEGRLKVHGTGGRFPNRSADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLVDALRIAGADVEYLGNEHYPPLHIGKRQDCGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVAN
                     EGYRVFKIPADAHYHAPEHLHVEGDASGASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKGRAVQAFDLDANHIPDAAMTLAIVALATRQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLISLLGVPVVINDPKCTHKTFPTYFEVFSSLTETAE"
     gene            complement(1541..2074)
                     /locus_tag="ACSIGJ_001761"
     CDS             complement(1541..2074)
                     /locus_tag="ACSIGJ_001761"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HHQ1330578.1"
                     /translation="MFSPDKTLFLCLGALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIGRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPGSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
     gene            complement(2131..3657)
                     /locus_tag="ACSIGJ_001762"
     CDS             complement(2131..3657)
                     /locus_tag="ACSIGJ_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HHQ1330579.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVLLDNI
                     LQIRHTPHNNGLSDIYLLDDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     MYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     RIDWQSVQTRLISDNPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKDLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDKHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
     gene            complement(3769..5310)
                     /locus_tag="ACSIGJ_001763"
     CDS             complement(3769..5310)
                     /locus_tag="ACSIGJ_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HHQ1330580.1"
                     /translation="MIFDNNYHYNAMPIRLLVNIQDADSAGIQTASENNNGTRMGYNH
                     PMEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADLNESPPDMQQAVVA
                     YTLTVALLIPLSSYLADKFGTKKVFFGSIAVFMLGSALCAASGSLFELALSRVVQGIG
                     GSMLVPIPRLTILRVYEKSKLLNAINYAVMPALIGPALGPLAGGYLVEYASWHWIFLL
                     NLPIGLLGFILGRNIMPDVKGNDTALDFKGYLTFSVAACLLLLAAESLSHALPPYFAL
                     LPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFSRLGISSIPFLMPL
                     MFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLLWNTKLLAAFIMLL
                     ALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGSGNSLMAVNQQLAI
                     SVGIVAGALILKNWTFLIPASSGLHFAFRMTLLSIGGITLASSLVFKRLHVSDGANLT
                     RGTRP"
     gene            5458..6237
                     /locus_tag="ACSIGJ_001764"
     CDS             5458..6237
                     /locus_tag="ACSIGJ_001764"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HHQ1330581.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAIALIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKNTEYESKAVDCANRIIEFTHFLLAIGYKPEDKGEPVKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKV"
     gene            6234..6935
                     /locus_tag="ACSIGJ_001765"
     CDS             6234..6935
                     /locus_tag="ACSIGJ_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutC/YkgG family protein"
                     /protein_id="HHQ1330582.1"
                     /translation="MSARENILAKLKKAGALPMEEPAVFDYYREKGVSWDSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALAGSNID
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            6932..8386
                     /locus_tag="ACSIGJ_001766"
     CDS             6932..8386
                     /locus_tag="ACSIGJ_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HHQ1330583.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHIRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDVPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(8535..8954)
                     /gene="fitB"
                     /locus_tag="ACSIGJ_001767"
     CDS             complement(8535..8954)
                     /gene="fitB"
                     /locus_tag="ACSIGJ_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin FitB"
                     /protein_id="HHQ1330584.1"
                     /translation="MILLDTNVISEPLRPQPNERVVAWLDSLILEDVYLSAITVAELR
                     LGVALLLNGKKKNVLHERLEQSILPLFAGRILPFDEPVAAIYAQIRSYAKTHGKEIAA
                     ADGYIAATAKQHSLTVATRDTGSFFAADVAVFNPWHD"
     gene            complement(8954..9190)
                     /gene="fitA"
                     /locus_tag="ACSIGJ_001768"
     CDS             complement(8954..9190)
                     /gene="fitA"
                     /locus_tag="ACSIGJ_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin FitA"
                     /protein_id="HHQ1330585.1"
                     /translation="MASVVIRNLSEATHNAIKFRARAAGRSTEAEIRLILDNIAKAQQ
                     TVRLGSMLASIGQEIGGVELEDVRGRNTDNEVSL"
BASE COUNT         2246 a         2431 c         2608 g         2077 t
ORIGIN      
        1 aaaaaaattt tagaaacaaa aaatttaaaa aaaatcaatt ttcggcataa aaaaccaaat
       61 tcacgtactt taaaaccgaa aatgccaaac ctgagatttt ccagacggca tttgcaccag
      121 tataatgcag gctgttttta tctttatatt tacgttttgc catgaccgaa tccgtccgcc
      181 tccccgccgc ctcgctcaaa ccttccaccg tcgccctgcc cggctccaaa agcatcagca
      241 accgcaccct gctgctcgcc gccctgtccg acaatgtttg cgaaatccat tccctgctca
      301 aatccgacga taccgaccgt atgctcgaag cactcgataa actcggcgtt caaatcgaac
      361 atcttgccga aggccgtctg aaagtacacg gcacgggcgg acgcttcccc aaccgctctg
      421 ccgatttgtt tttgggcaac gcgggcacgg cgttccgtcc gctgaccgcc gcgctggccg
      481 ttttgggcgg cgattatcat ctgcacggcg tgcctcgtat gcacgaacgc cccatcggcg
      541 atttggtcga tgcgttgcgg attgccggcg ccgatgtcga atatctcggc aacgaacact
      601 atccgccgct tcatatcggc aaacgtcaag actgcggcga gcgcgtgatt cctattaaag
      661 gcaatgtgtc cagccagttt ctgaccgccc ttttgatggc gttgccgctg accgggcagg
      721 cgtttgaaat ccgtatggtc ggcgagttga tttccaagcc ctatatcgac attactttga
      781 aactgatggc gcaattcggc gtacaggttg ccaatgaagg ctaccgcgtt ttcaaaatcc
      841 ctgccgatgc gcactatcac gcacccgaac acttgcacgt cgaaggcgat gcctccggcg
      901 cgtcctactt cctcgcagcc ggtttgattg ccgccacgcc cgtccgcgtt accggcatcg
      961 gcgcaaacag catacagggc gatgtcgcct ttgcccgcga actggaaaaa atcggtgcgg
     1021 acgtggtttg gggcgaaaac ttcgtcgaag tctcgcgccc gaaaggacgc gccgtccaag
     1081 cctttgattt ggatgcgaac catatccccg atgccgcgat gaccctcgcc atcgtcgcgc
     1141 ttgccacaag gcaaacctgc acgcttcgca acatcggttc gtggcgcgtt aaggaaaccg
     1201 accgcatcgc cgcaatggcg aacgagttgc gcaaactcgg tgcaaaagtc gtcgaagaag
     1261 ccgaagcgat tcacattacc ccgcccgaaa cgccgacacc cgatgccgtc atcgacactt
     1321 acgacgacca ccgcatggcg atgtgtttct cgctgatttc gctgttggga gtacccgtcg
     1381 tcatcaacga cccgaaatgc acccataaaa ccttcccgac ttattttgaa gtgttctcat
     1441 cactgaccga aacagcggaa taaggcggca aaatgccgtc tgaaacccga aaacagggct
     1501 tcagacggca ttttgccgcg attccggcgc ggcgggcggc tcattctgta aaaaacgtat
     1561 gcgctccaag gtagtttttg gcgtaaaacg gtgtggagag tttttcggtt ttgatggttt
     1621 tgccgctgcc gggggcatgg atgaattcgc cgttgccgat gtagagtccg acgtgtgagt
     1681 agcggtgtgc gccgccggtg ttgaagaata cgaggtcgcc ggccttgagg cggctgtcgg
     1741 ggattttgcg gcttgccgcc gccatgtcgc gggcggtgcg cggcagcttg acgttgaggg
     1801 cgtttttgta aacgaattga atcatgccgc tgcagtcgaa gccggttgcg gtgctgctgc
     1861 cgccccattt gtagggcgtg ccgatgagtc cgaggctgtg gagcatgagt tcctgcgagc
     1921 cttgtgtgcg gccgatgtgg ctgatgcgga cggcttggat ttgccggact gtctgtttgg
     1981 gtttcggttg gcggtgtttg ccggaggtcg tgccgcatga ggcgaggagc agtgcgccga
     2041 gacagaggaa aagggttttg tcgggggaaa acatggtttt ttcctttgcg ggttcggata
     2101 tgccgtctga aggtgtttca gacggcattt ctataataaa ccttcgatgg gcagcaggga
     2161 taggattttt gcggcgatgc gtttccaaag tttggcttcg ggttcgttcg ggtaggtttt
     2221 tcgggtggcg ggatcgtgcc attgcaggcg gttgtgtttg tcgagggtaa cgcggtaggc
     2281 gtattcgggt gtggtatcgg cgagggtgcg ctccatctgt tctgcgattt tggggctttc
     2341 gatgacgacg cccatttcgg tattgagccg tgcggaacgg gggtcgaggt tgaatgagcc
     2401 gatgaagatg cgtttgccgt ccacaatgaa ggttttggca tgcaggctgg ttacggagct
     2461 gccggtcagg tctttgtctt ttgtggcggg gacggcatgg ttgggttgca gctcgtagag
     2521 tttgatgccg gctttgagca gcggttttcg gtatttgacg tagccggaat ggacggcggc
     2581 aacgtcggtc gcctgtagcg agttggtcag gacggtaacg tctatgccgt cctgcaccag
     2641 ttttgccagt gcgtctgtgc cggattttgt agggacgaaa tagggtgaaa ccagatagac
     2701 gcttttttcg ggctgtttga gcgcgtcttg cagcctcccg gcaatcggcg gtttgcggcg
     2761 gtcgcggtcg agtccttttg cagggttgtc gctgatcagg cgggtttgga cgctctgcca
     2821 gtcgatgcgt cccgtctgta ttttttggta gaggggcgac tgttcgacgg tttcgcggta
     2881 gcgcaggagc gcgtgtctgg atgtttcgtc gttgtatccg agtgcttgaa gacccttgcc
     2941 gatgttgccg ctgcggatga tgcgcgtggc gttgtgggcg gaatggcttg cccagtagcg
     3001 gtcgaagtcg tgcgatactt cgccgacgac gctgccggtg gcgaggatgt ccaggtcggc
     3061 gaaaacggtg tcctcaccga ctttgaagta ttcgtcgccg atattgcgtc cgccgagtat
     3121 ggtggcgcgg ttgtcggcgg taaaggattt gttgtgcatg cggcggttga ggcgggggaa
     3181 gtcggtcagg tagccgagtg cgcgccattt gcgtaggacg aaggggttga acaggcgcac
     3241 ttcgatattg ggatggctgt cgagggcgag caggagatcg tccaacccgc gcgtgttgtt
     3301 gtcgtccaac agcaggcgta cgcgcacgcc gcgttctgcg gcaaggtaca tgaggttgaa
     3361 cagcagcctg ccggaaatgt cgttgcgcca aatgtagtat tgcaaatcga ggctgtgttc
     3421 ggcagattcg ataagggcgg cgcgggcggc aaaggcttcg tgggggtcgt cgagcaggta
     3481 gatgtcggat agcccgttgt tatgaggggt gtgccggatt tgcaggatgt tgtccaggag
     3541 gacaggtttg gaagtattga aatgacggct ttccgtccgt tcttccagtg ggggcaacca
     3601 tgaagaacat gaacagagaa ggaggcataa aagggaaatg aggctgcgtg ttttcatcgg
     3661 ggatatagtt tcagacggca ttgcctgtat tttggggtct gtatgcattg aaatgaagca
     3721 tcataatcta aaggttggaa tgggtaaaag ttttgtcggg atgcggggtt aaggccgcgt
     3781 gcctcttgtc aggtttgcac cgtctgaaac gtgcagacgc ttaaaaacca acgacgaggc
     3841 aagggtgatg ccgccgatac tgagcagggt catacggaag gcgaaatgca ggcctgaaga
     3901 agccggtatc agaaatgtcc agtttttaag gattaatgcg ccggcaacaa tgcccacgct
     3961 gatggcaagc tgttggttga ccgccatcag gctgttgccg ctgccggtct gttgcgggcg
     4021 caaatcggcg agggtcagtg tgttcatggc agaaaactgt agggagttgc atgcgccgat
     4081 cgccagcgag aggaaaatcc aaatccacag cggcgagttt ccgtcaggca gggcgagcag
     4141 catgatgaag gcggcaagca gcttggtgtt ccaaagcagt accgtgcggt agccgaaacg
     4201 tttcatgagc ggtgcaatca gcggtttgac cagcagcgaa gacagggcga cgggtgcgac
     4261 cagccagccc gacaggcttg cgccgaagcc gaaagcgact tgaaacatca ggggcatcag
     4321 aaaaggaatc gagctgatgc cgagacggct gaacagattg cccgccagtc ccaaacggaa
     4381 ggtgcgtatc agaaacaggt cggcggaata aatcggtttg gacgcggttt tcatatgtcg
     4441 gaaataacgg cgtgcaaaca gcagtccgcc gcacagcggt aacagtgcaa aatacggagg
     4501 cagcgcgtgc gacaggcttt ctgccgcaag cagcagcagg caggcggcaa cggaaaaggt
     4561 cagatagcct ttgaaatcca gagcggtatc gttgcctttg acatcgggca tgatgttgcg
     4621 tcccaatatg aaacccagca gaccgatggg caggttgagc aggaaaatcc agtgccacga
     4681 agcgtattcg accagataac cgcccgccag aggtcccaac gcagggccga tcagtgcagg
     4741 catgaccgca taattgatgg cgttgagcag cttcgatttt tcatatacac gcaagatggt
     4801 cagacgcggt atcggaacca gcatcgaacc gccgatcccc tgaacgacac gggaaagcgc
     4861 caattcaaac agcgaacccg atgcggcgca caatgccgat ccgagcataa aaacggcaat
     4921 cgaaccgaaa aagacttttt tcgttccgaa cttgtccgcc aaataactgc tcaaaggaat
     4981 cagcagggca accgtcagcg tataggcaac gactgcctgt tgcatatccg gaggcgattc
     5041 gttcaggtcg gcggcaattt cgggcagtgc ggtatttaaa atggtcgcat ccaacatctg
     5101 cataaaaatg gcaattgcca gcagaagcgg cagccaaggg gatggtgcgc gggcggacag
     5161 ggtgtttttt tccatagggt gattgtaccc catccttgtg ccgttattgt tttcagatgc
     5221 tgtctgaatg ccggcagagt cggcatcctg aatgttcaca agcaaacgaa tcggcattgc
     5281 attgtaatga taattattat cgaaaatcat cagagtaagg tacagtaagc gttatggggc
     5341 agtttgtaag aaaaaccgga ttatttttta aaattagact tgacccgcaa cggtcaatta
     5401 cttaaagtaa acgcttacct ttctacagaa aaaacgggtt tcccgttatc aaaaaatatg
     5461 agcgcaacca ttcccccaaa aatcatccga tacgacagca atccgacaga tgtctatttt
     5521 ttcggcactt gcgtccttga tctttttatg cccgaagcag gcatggatgc cattgcccta
     5581 atcgagcagc agggcatacg cgtccatttc ccgatggcgc aaagctgctg cggtcagcct
     5641 gcctattcat ccggccatcc gaccgaagcc ttcgatgtcg ccaaagcgca actcgacctt
     5701 ttccccgaaa actggccgat tgtcgtaccg tccggctcat gcggcggcat gatgaaacat
     5761 cactggccta ccctgtttaa aaatacagaa tacgaatcca aagcggttga ttgtgccaac
     5821 cgtatcattg agtttactca tttcttgctt gccatcggct acaagcctga agacaaaggc
     5881 gagccagtca aagtcgccgt tcacacttcc tgtgccgccc gccgagaaat gaatgtccat
     5941 ctttcaggct ggcagttgat tgacggtatg gaaaacgtcg aacgcatcgt ccacgaccac
     6001 gaaagcgagt gttgcggctt cggcggcaca ttctccgtca aacaagccga tatttccggc
     6061 gcgatggtaa ccgacaaagt tgccgcgctg aaagaaaccg gcgcaaccga aatcatcagc
     6121 gcggactgcg gctgtatgat gaacatcgga ggcaaaatcg ccaaggacga gccggatatg
     6181 ccgcgtccga agcatatcgc atccttcttg ttggaacgca ccggaggcaa agtatgagcg
     6241 cgcgcgaaaa tattttggca aaactgaaaa aagccggcgc attgccgatg gaagagcctg
     6301 cggtttttga ttattaccgc gaaaagggcg tttcttggga cagcgaagcc gagcgtctga
     6361 aacattgggc tgccgccatg cgcgcggtca aaaccgaaat ctattgggtg acgaaaagca
     6421 attggatgca ggttttccgc gaagcggcag aaggcaaggg tttaaaaaac atcctgctgc
     6481 ccttggcgac cgaacacgga caaattgccc gtgccgcatt ggcgggcagc aatatcgacc
     6541 cgattgcctt cgagcgcgaa atcgatactt ggaaaaccga gtttttcacg aacatcgatg
     6601 caggcttcag cggcgcgcaa tgcggcatcg cccgcaccgg cacgctgatg ctgttttcca
     6661 gccctgaaga accgcgtact ttgagcctcg ttccgcccgt gcatttctgc ctgttcgata
     6721 cgtccaagat gtacaacgag tttcacaacg ccgtcgaagg cgaaaaactg gtggaaaacg
     6781 gtatgccgac caatgtattc ctgatttccg gcccgtccaa aaccgcagac atccagttga
     6841 cgctcgctta cggtgcgcac ggtccgcgtg atttggtcat cctcgccatt ttgcccgacc
     6901 acatttcccc tgccgatttg gaggaaaacg catgactacg caaaccatca agttccacat
     6961 gaagccggaa actttcaaac aaaacgccgc aatttccctt caagacaagc ccttgcgtaa
     7021 aagcctgcgt accgcgatgg atatgctgat gaccaagcgc aaagccgttt tgaccgacga
     7081 agaagagttg caaagcctgc gcgatttgtg cgaacacatc cgtcagcgtt ccctctccaa
     7141 attacccgcg ctgctggagc agttggaaga aaacctgact aagttgggcg tgaaagtaca
     7201 ctgggcggaa acccctgccg aagcctgcca aattatccac gacatcatca cagccaaaaa
     7261 cggcaagctg atggtcaaag gcaaatccat ggtcagcgaa gaaatcgagc tgaaccatta
     7321 tcttgaagca aaaggcatta aggcggtgga aagcgacttg ggcgagttta tcgtccaaat
     7381 ggcaggcgaa aaaccgaccc atatcgtgat gcccgcaatc cataaaacca aagaacaggt
     7441 cagcgaactg ttccaccaaa acctcggcac accgcttaca gacgacgtgg accaactgac
     7501 cggattcgcc cgcaaagccc tgcgcgatat ttacagcact gccgatgtcg gcttgagcgg
     7561 cgtaaacttc gccgttgccg aaacgggcac gctgtgtctg gtcgaaaacg aaggcaacgg
     7621 ccgcttgagt actaccgttc cgcccgtaca tatcgccatt acgggtattg aaaaagttgt
     7681 cgccaagctg tctgatgttc cgcctttgta cagtctgctg ccgcgttccg ccatcggtca
     7741 gaacattacc acttatttca atatgattac cggcccgcgc cgcagtgaag aattagacgg
     7801 tccgcaggaa atgcacttgg ttttgctcga caacggccgc agccaagctt atgccgaaga
     7861 ccaaatgcgc cgcaccctgc aatgtatccg ttgcggcgcg tgtatgaacc attgcccggt
     7921 ttatacacgc atcggcggcg cggcatacgg cacaacctat cccggtccga ttggcgagat
     7981 tatttccccg cacctgttgg gtcttgatgc cacccgcgac ctgccgaccg cctgcacgat
     8041 gtgcggcgcg tgcgtggaag tttgtccggt acgcatcccg attaccgaac aaatgcaacg
     8101 tttgcgcgtt gaagcacaac gttcgccgac cgaaaccgtg ccgcacccca tccgagggca
     8161 aggcgcatcg cataccttcg gcgaacaaat ggcgtggcgc acattcaacg gtattttcag
     8221 cggtagcaaa acctaccgcg ccttcggttg ggcagccacc aagttccgca atctgacccc
     8281 gcgcaaacag ttgggttgga cgcaaaaccg cgtaccgatg aaaccggcga agaaaaccct
     8341 gcacgaactg atggcggaaa aaatgcgcca aaaagaacag gcataaaaag ttgttcgcaa
     8401 aaataccgtc tgaaacccga aaacagggct tcagacggcc tgaaaagcag cctgaacgcg
     8461 caaaaataaa taatgaaacg gaatgctaag acgctttcag gcagcctgac attaaggctg
     8521 cttaaaagcg tcttttaatc gtgccacgga ttgaacaccg cgacatcggc cgcaaaaaat
     8581 gagccggtat cacgcgtagc aactgtcaaa ctgtgctgtt ttgcagtggc ggcaatatag
     8641 ccgtctgcgg cagcaatctc tttgccatgt gttttggcat aggaacgaat ttgcgcatag
     8701 attgcggcaa ccggttcatc aaaaggcaga atccgccccg caaataaagg caaaatggat
     8761 tgttccaaac gttcgtgcag cacattcttt ttcttgccat tgagcaacaa cgccacaccc
     8821 aaacgcaatt ctgcaacagt aatggcagac aaatacacat cttccaatat caaactatcc
     8881 aaccatgcca ccacacgttc attgggttgt gggcgcaaag gttcggaaat cacattcgtg
     8941 tccagcaaaa tcatcacaaa gaaacctcgt tatcagtatt acgaccgcgt acgtcttcca
     9001 gctcaacacc tccgatttcc tgccctattg atgccaacat tgaccccaaa cgtacagttt
     9061 gttgtgcttt ggcgatgtta tccaaaatta agcggatttc tgcttcggta ctgcgccctg
     9121 cggctcgcgc acggaatttg attgcgttgt gcgtggcctc ggataaattt ctaatcacaa
     9181 cagaagccat tgcctttcct tcctgaaaaa agtttttcaa atgctatcaa aataaaaaaa
     9241 atgatagcaa tctatttttt aacgcctgcg gatgcctgaa aattcaggtg gccgcaggcg
     9301 tttttttaca tctaaaaaaa cgtctcccct ccctctttct gataaataaa aaacagtcgc
     9361 tc
//
LOCUS       DBJGGI010000071         9006 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.071, whole genome shotgun sequence.
ACCESSION   DBJGGI010000071 DBJGGI010000000
VERSION     DBJGGI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9006
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.071"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(122..421)
                     /locus_tag="ACSIGJ_001769"
     CDS             complement(122..421)
                     /locus_tag="ACSIGJ_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330586.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERYGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFILARQGHGVVVAMQ"
     gene            complement(421..1722)
                     /gene="nuoF"
                     /locus_tag="ACSIGJ_001770"
     CDS             complement(421..1722)
                     /gene="nuoF"
                     /locus_tag="ACSIGJ_001770"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HHQ1330587.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVRRIVEGKGRMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFAHYIEHGGPMKEHKWGGW"
     gene            complement(2112..2585)
                     /gene="nuoE"
                     /locus_tag="ACSIGJ_001771"
     CDS             complement(2112..2585)
                     /gene="nuoE"
                     /locus_tag="ACSIGJ_001771"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HHQ1330588.1"
                     /translation="MLSAKSLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            complement(2585..3841)
                     /gene="nuoD"
                     /locus_tag="ACSIGJ_001772"
     CDS             complement(2585..3841)
                     /gene="nuoD"
                     /locus_tag="ACSIGJ_001772"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HHQ1330589.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPGFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCADWLRVNPGPVITANHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            complement(3831..4424)
                     /locus_tag="ACSIGJ_001773"
     CDS             complement(3831..4424)
                     /locus_tag="ACSIGJ_001773"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HHQ1330590.1"
                     /translation="MASIQNLYETVVGVLGDQASKVISALGEITVECLPEHYVSVMTT
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            complement(4437..4919)
                     /locus_tag="ACSIGJ_001774"
     CDS             complement(4437..4919)
                     /locus_tag="ACSIGJ_001774"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NuoB/complex I 20 kDa subunit family protein"
                     /protein_id="HHQ1330591.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            complement(4910..5266)
                     /locus_tag="ACSIGJ_001775"
     CDS             complement(4910..5266)
                     /locus_tag="ACSIGJ_001775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HHQ1330592.1"
                     /translation="MLSAYFPVFVFILVGLAASVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            complement(5729..7180)
                     /locus_tag="ACSIGJ_001776"
     CDS             complement(5729..7180)
                     /locus_tag="ACSIGJ_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HHQ1330593.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLNPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            complement(7203..7823)
                     /locus_tag="ACSIGJ_001777"
     CDS             complement(7203..7823)
                     /locus_tag="ACSIGJ_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HHQ1330594.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
     gene            complement(8022..8399)
                     /locus_tag="ACSIGJ_001778"
     CDS             complement(8022..8399)
                     /locus_tag="ACSIGJ_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HHQ1330595.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWNRAKRHLRKFNGIPKAHFELYLKECEW
                     RFNNSEIKVQISILKQLVKSSLS"
     gene            complement(8708..8833)
                     /locus_tag="ACSIGJ_001779"
                     /pseudo
     CDS             complement(8708..8833)
                     /locus_tag="ACSIGJ_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         2362 a         2240 c         2216 g         2188 t
ORIGIN      
        1 taactcattt attatatttt taaatgactt aaataactct ttaacagcac gctcattgtc
       61 ttcatctgta ggctcaaact tttcacacca attaattata tttacatcgc catgcaatat
      121 cctactgcat cgcaactacc accccgtgtc cttgacgtgc caaaataaaa tcacaattat
      181 gcgaataaag tttgtataaa tttctcctaa cctcgtattc attatcacca gatgaataaa
      241 aaccaatcct ctgaccttca tgatcaaata ctgcaacaaa atctaagccg tttccatatc
      301 tttcaaaaaa gactagaaat ttagaaaatt tccgacaaat ctgattaaaa agcgtattga
      361 gtgtcgtact cttacctttg ttggctgcac cgtatagaat aaaaatttta gcttccacca
      421 ttaccaccct ccccacttat gctctttcat cggcccgccg tgttcgatgt aatgcgcaaa
      481 ctcatcacgg aaatgcttgg taaagctgcg gacagggaag acggcggcat cggcaagggc
      541 gcagatggtg cggcctgcca tttggttgcc gacagaatcc agcaaatcca agtcttccat
      601 gcggcctttg ccttctacga tgcggcggac gatgcggtaa agccagccgg taccttcgcg
      661 gcagggggtg cattggccgc aggattcgtc gtagtagaaa tagcttaagc gttcgagggc
      721 tttgaccatg cacacgtctt cgtccatcac gataatcgcg ccggaaccga gcatcgagcc
      781 ggctttggaa atcgagtcat agtccatatt ggtctgcatc ataatgtcgg caggcaatac
      841 gggggcggac gaaccgccgg gaataacggc tttgagtttt ttaccgccgc gcataccgcc
      901 cgccattttc aagacttcgg caaacggcgt gcccaacggc acttcatagt tgcccggacg
      961 ctcgacatgg ccggaaatac aaaataattt ggtaccgcct gcattcggaa tacctttatc
     1021 ggcaaacgcc tgtccgccgt cacggataat gaatggaacg gaggagaacg tttcggtatt
     1081 gttgatggtg gtcggtttgc cgtacaggcc gaacgaggcg gggaacggcg gtttgaagcg
     1141 cggctggcct tttttgcctt ccaacgattc aagcaatgcg gtttcttcgc cgcaaatata
     1201 tgcgccgtag ccgtggtggg cgaagagttc gaattcaaaa tccgaaccca aaatattttt
     1261 acccaaaaag cctgcggcac gcgcctgctc caaagcggct tcaaagcgtt gataaccttc
     1321 gaaaatttcg ccgtggatat agttgtagcc ggctttcgcg cccatcgcgt aaccggcaat
     1381 gatcatgcct tcaatcaggg catgaggatt gaacatgatg atgtcgcggt ctttaaatgt
     1441 acccggctcg ccttcgtcgg tgttgcaaac cacatatttt tcgcccggga aagaacgggg
     1501 cataaagctc catttcaaac cggtcgggaa gcccgcaccg ccgcgcccgc gcaagccgga
     1561 ggttttgact tcgtcaatca catcggtttg cgagatgttt tcggacagga ttttacgcag
     1621 ggcggtatag ccgccgcgtt tgacgtattc gtccaatgtc cagcaatcgg gattggcggt
     1681 atccacttgg tcaaaaatca cgcctgattg gtaaatagcc atttttggtg tgcctgtttg
     1741 ttttcgtatc ggttgcggcc gctgtttcag acgaccttaa aatgtctttg tgtatcggct
     1801 tgtaacgtct tctgaaataa aatctagttt atcaaatcga tattttcaga cgaccttcaa
     1861 tcgtttttgt tggatctcga ccacttgctt gtctgccgtc attcccgcgc aggcgggaat
     1921 ccatcctcaa tggtaagcaa tgtcttatta aattcagaaa ccgaatctta ccggtggatt
     1981 cccgcctgcg cgggaatgac ggcatttcag tatttcagta gggcggattc ttaaatccga
     2041 cattttgcct ttttacccac tctgtcggat acaagtatcc gacctacgtt taaatcgtcg
     2101 tttcagacga cctactccaa ctccgccagt ttcttctcaa tcgcctcttc ggtcataaag
     2161 ctgcacatgc tgtggttgtt gaccagcata acgggggcat cgccgcacgc gcccatgcat
     2221 tcgccttcga caagggtaaa tttgccgtcg ggcgtggttt cgccgtagcc gataccgagt
     2281 ttttgtttga ggtattcgcc ggtcgccata ccgccgcgca gagcgcaggg caggttggta
     2341 caaacggtca gtttgtattt gccgacaggc tcaaggtcgt acatattgta gaaagtggcg
     2401 acttcatatg cctgcgccgg cgtgatgccg atgtagtcgg caacaaaagc gatggtttcg
     2461 ggggcgagcc agcctttttc ggtttgggcg atacgcaacg cgcccatgat ggcggaacgg
     2521 cgttggtcgg cgggatattt cgccaactca atgtcgattt gttttaagga ttttgcggat
     2581 aacattatcg gtcaacctcc ccgaatacga tgtcctgcgt accgatgatg gcaacgacgt
     2641 cggcgagcat atggcctttt gccatttcgt ccatgccttg caggtgggcg aagccgggtg
     2701 cgcggatttt caggcggtag ggtttgtttg cgccgtctga aatgatgtaa acgccgaact
     2761 cgcctttcgg gtgttcgaca gcggtgtagg tctcgccctc gggaacgtgc ataccctcgg
     2821 taaagagttt gaaatggtga atcaggtctt ccatacctgt tttcatttcg gtacgtttgg
     2881 gcggagcgaa tttgtggttt gcggtaatga ccgggccggg attgacgcgc aaccagtcgg
     2941 cgcattgttt gatgatgcgt acggattggc gcatttcttc catacggcag aggtaacggt
     3001 cgtagcagtc gccgttgacg ccgacgggga tgtcgaaatc cattttgtcg tacacttcgt
     3061 agggctgtgt cttacgcacg tcccattcca cgccggaacc gcgaagcatc acaccggtaa
     3121 agcctttttg catggcgcgc tcgggagaga caacgccgat gccgacggta cgctgtttcc
     3181 agatgcggtt gtcggtcagg agggtttcga gtgtgtcgat atttttgggg aagcgttcgc
     3241 agaaggcatc gataaagtca agcatcgtgc cttcgcggga ttcgttgagc tgcttcaata
     3301 ctttggcatt gcggaatttg ctgctctcgt atttgggcat aaagccgggt aggtcgcggt
     3361 aaacgccgcc gggacggaag taggcggcgt gcatacgtgc gccggacacg gcttcgtaca
     3421 agtccatcag ctcttcgcgg tcgcggaagg cgtagaggat ggcggtcatc gcgccgatgt
     3481 cgaaggcgtg cgaaccgatg cccatcaagt gattgaggat gcgcgttact tcggcgaaca
     3541 tcacgcggat gtattgggcg cggataggca catcgatacc ggcaagtttt tctaccgcca
     3601 agcaatacgc ctgctcgttg accatcatgg aaacgtagtc caaacggtcc atatagggca
     3661 gggcttgcag ataggttttg gtttccgcca gtttttcggt gccccggtgc aggaggccga
     3721 tatgcggatc ggcacggacg atttgttcgc cgtccagctc caaaatcata cgcaatacgc
     3781 catgcgccgc agggtgctgc gggccgaagt tgatggtgta gtttcttaat ttattggcca
     3841 ccgtagttct cctcacggac gatacgcggc gtgatctcgc gcggctcaat ggtaacaggt
     3901 tggtaaatca cgcgtttttg ctcttcgtcg taacgcattt ccacatagcc ggaaatcggg
     3961 aagtctttgc ggaacggatg tccgacgaaa ccgtaatcgg tcaggatgcg gcgcaagtcc
     4021 ggatggttgt tgaacatgat gccgtacata tcgaaggctt cgcgttcgta ccaatccgcg
     4081 ctgttgtaaa tatcgactac agattcgact acggggaagt cgtcgtctga aacccagacg
     4141 cgcacgcgga tgcgttgatt gtttttaacg gaaagcaact gactgacgac ggcaaagcgt
     4201 ttgccctgcc atacttcgtt tttgtaagtg ctgtaatcga cgccgcacaa gtcaactaga
     4261 agctcgaaat gcaactcttc atggtcgcgc aacgtagtca tcactgaaac atagtgttcg
     4321 ggcagacact caacggtaat ctcgcccaaa gcagaaatga ctttgcttgc ctgatcgcca
     4381 agcacgccga caacggtctc gtataagttt tgaatgcttg ccatatcgtc ctctccttac
     4441 tcgtcacgcg cgatggtgga agtgcgcttg attttttgtt ggagctgaat caggccgtaa
     4501 atcagggctt ccgcagtcgg cggacaaccc ggcacataaa cgtctaccgg cacgacgcgg
     4561 tcggcaccgc gcacaacgga ataagaatag tgataatagc cgccgccgtt ggcacacgag
     4621 cccatagaca atacccagcg cggctcggcg agctggtcat acacgcggcg cagggcgggt
     4681 gccattttgt tggtcagcgt acccgccaca atcatcaggt cggcctgacg tggggacgga
     4741 cggaaaatga tgccgaaacg gtcaaggtca taacgcgcca tacccgcgtg catcatttcc
     4801 acggcgcagc aggccaagcc gaaagtaacc ggccacaacg aaccggtacg catatagttc
     4861 aacaccgtat ccgcgctggt ggtgatgaaa ccttttttca aaacgccttc tattcccatt
     4921 ccagcgcacc ttttttccat tcgtaaacaa agcctaccgt cagaacgacg ataaacacca
     4981 gcatagacca gaagccgtac gcgcccaaat ctttgaacac gactgcccac ggcaacatga
     5041 acgcgacctc caaatcaaac aagataaaga ggatggcgac gaggtaatag cgcacgtcga
     5101 acttcatcct ggcgttttca aaggcttcaa aaccgcattc gtaaggcgcg tctttttcgg
     5161 cgtagtggcg tttcgggcct aaaatcgtgc cgagcaggat aaacagcacg ctggccgcga
     5221 ggccgacgag gataaagaca aagacgggaa agtaagcgga caacatggtt acacccaaat
     5281 ccgttaacaa aatttctaca ataatttcgt attttagcga atttcaaaaa ccattaaaag
     5341 gtaaatatcg gcaaaacgcc caaaaaaacc ccaataaata caatcatgtt atgataacga
     5401 ttcttatttg attttataag gtcatataaa tttttacacc gtaattcgtg gtatagtgga
     5461 ttaacaaaaa ccagtacggc gttgcctcgc cttagctcaa ggggaacgat tccctaaggt
     5521 gcccaagcac caagcgaacc ggttccgtac tatttgtact gtctgcggct tcgtcgcctt
     5581 gtcctgattt ttgttaatcc actatatata ggaataagaa ttcgggacaa aatgtttaaa
     5641 ccattttgtc ccgactgctg tgatgcggtt tttttgaact agcaggacgt tacctaaaac
     5701 ctgcgttgcc tgaaaccctt taagacggtt aaatccctct gccgtatttg tattctacaa
     5761 tcatattatc gtcggtaata acttccgccc cagccgaagg ttccgtcatc tgaatcagca
     5821 tacgagagac aaccttttgt gctgcagcat ccacggtgct gctgtcaaat acgtgcctgc
     5881 cgctttccgg ccaaatcaac cgggagagac gttgcttgag cagttcttta ttagggaaaa
     5941 ctaccggggt tgccgagccg actaccatat gcccatagcg gtatgcatag ggaatactgt
     6001 gtacggcggt agcaaaagca tgcgggctgt gcgtggtatt aaacattaca ataccatccg
     6061 ggttaaggtg gctttgcacc tgttttaaaa attccgcact caacaggttg gtggaatagg
     6121 cacgccagta ccaagtcgta ttcatcaaaa tcaggtcgaa tttttcatca ggatggcgac
     6181 gcagccattt cctaccgtca tccaatacaa tttcaacacg tttgtcctgc aaaagcgggg
     6241 cgatttgcgg ctcgtccgcg ataaggctac ggtatgccgg attgatttcc gcaacgatca
     6301 tcgactgcat ttccggaatg gcagacaaga cgcgcgccca cgaacctgta ctcaatccaa
     6361 cgacgaaaat gcggcgtatg ccagacttca gggagggtag cagataggca cgttcgatgc
     6421 cgttgacact attgaataca tcggtattgt atgcgccgtc gtatacattc gccccataaa
     6481 caaccttatc accatctcta tggtaaaccg caacaatgcc gtgtttgttt tcaatcagcc
     6541 tatccggacg gtcagcaata ttttgaaaga cagaatccgg cagtaggaac atgaggatgc
     6601 cgaacattag ggaaactgct accgacactg cattcagtcg gagacttttt tggaacagtg
     6661 tacaaaacaa agggacagca gcagaaatca aacagatgag caggtaaatc tgttgggtgg
     6721 acaagaaatc aagtatcaca aagccgataa ggaccggacc caatgcactg ccggcaacgt
     6781 tggcgaaata aacattggaa acctgtcgtc cggatttgtt gccatccgta cccacatggt
     6841 gtacgagcgg gaaaatcaac cccctgacga cggcagacag ggtaatgaag ataccggcgt
     6901 ggtggacgaa gccggaaaaa cccgtcaaca accacgcagc acccaaaatc aaaaagtcgg
     6961 caatacccgc ccacaagaag cactgcccga taaagggaat atcaacaaag cggctgcggc
     7021 aaatccgttt gccaaaatac gcgccgacgg cgataccggt cagaaaacag gcaagggtaa
     7081 atgaaaatgc ctgaggcacg gactgtgctg cgaacgaaaa catcctcacc cacaagactt
     7141 ctatacccaa gctcaataag ccgctaagga aagaaagcat ataaatcaaa ctagtattcg
     7201 gtttagtgtt cactatatcc atcctttctg taacgcagcc atactgaagc agcaatcaga
     7261 aggttaaagc aggctgtcag cgcaatggtt tgggagaggg taaaaaagac gtagaaaaat
     7321 tcggcggcgg caagcgatcc gagtgccgca cccaaagtgt tgaaaaaata taaggtaccg
     7381 atagactcgc caacattatg tattttccgg ttaaaaaaac aggtcagcaa gggcaaggtc
     7441 gcgcccatca taaaggtagg aagcagcaat aagaggaaat tggcagcagc gatgatgggc
     7501 aaatcagcct caactaaaag atgccccaag ccggaaatca gacccctgct taccaaaccg
     7561 aacagaccga tggatacttc agcgatgcaa aacaggggga tgatacttga aggaaaacgg
     7621 tcagcaatgc gtccaccgaa atacgcacct acacccaagc cgaccataaa tacagaaata
     7681 atgacagtaa tcgaactcaa atcgatacct atgtgactga atagaagcct ctgccagctg
     7741 acctggtaaa tcagggcgca gaatccagag gcgaaaaaca ccagcgacaa tcctttcagg
     7801 gttttacttg cggtcgaatt catattttct attttcaaat aggcggaact ataaatgaaa
     7861 tgtatcaatg tgtaaacatt tgtattaacc gtaggaaatc accgtattgc tcaaactgca
     7921 ggccgggaaa ataaactcgt aagcccgcaa acggcgtatc actgttcaaa ctgctgtgcg
     7981 gcttcacggt gttataaaaa taatggggct gtcctggaca actaagataa actcgatttt
     8041 actaattgtt ttaaaatgga aatttgaact tttatctcac tgttgttaaa acgccattcg
     8101 cactccttta aatacagctc aaaatgcgct ttgggaatgc cgttaaactt gcgtaaatga
     8161 cgttttgccc ggttccaaaa attctcaatt ccattgatat ggttttgtcg ttcagcaaaa
     8221 tgtgtgctgt gattgatacg gaaatggcta aattcgctca catctaatac atcatagcta
     8281 cgataacaat ccgtataaac aatgctgtca ggctctgttg ctgttcttgc agttacacct
     8341 gcgacaaata gctcaatgag tttattttgt ttataccggc ttagacgact ttttctcata
     8401 gggataattc taacttaatt tgaatttccc tatttatcta ggacagcccc aagaataata
     8461 atcattctgg atattcagtg ataacagaca ccgggtgttc ataccctgtt tttaccgtcc
     8521 gtcattccca cgaaagtggg gatccaggac gaaaaatctc aagaaaccgt tttacccgat
     8581 aaatttccgc accgacagac ctagattccc gcctgcgcgg gaatgacggt tcgggtattt
     8641 ctgacgattc atcgagccgt agcaactgta ttttccactc cgtcgggcaa aaataccaaa
     8701 actcaaatca agccgtccga ataccgtttt cggcggtatc gtttgcggca aaataatcac
     8761 gcatccgggc attcgatatc gtcggcagtt tgcgcataca tgccgtaacg gcaaccttat
     8821 acggcttacc cttgtatggc ggattaacaa aaatcaggac aaggcggcgg gccgcaggca
     8881 gtacaaatgg tacggaaccg atccgcccgg tgcttgggcg ccttagggaa ccgttccctt
     8941 tgagccgggg cggggcaacg ccgtaccggt ttttgttaat ccgctatacc tgcgtaatgg
     9001 tatcgg
//
LOCUS       DBJGGI010000072         9005 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.072, whole genome shotgun sequence.
ACCESSION   DBJGGI010000072 DBJGGI010000000
VERSION     DBJGGI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9005
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.072"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            129..619
                     /locus_tag="ACSIGJ_001780"
                     /pseudo
     CDS             129..619
                     /locus_tag="ACSIGJ_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase"
     gene            656..922
                     /locus_tag="ACSIGJ_001781"
                     /pseudo
     CDS             656..922
                     /locus_tag="ACSIGJ_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1424..2047
                     /locus_tag="ACSIGJ_001782"
     CDS             1424..2047
                     /locus_tag="ACSIGJ_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HHQ1330596.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLISPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPDEGERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGDIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            2159..3541
                     /locus_tag="ACSIGJ_001783"
     CDS             2159..3541
                     /locus_tag="ACSIGJ_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHQ1330597.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAIYAAS
                     LPGAEDNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLVVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PMIADVAGVRGLFMLTGILTAISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTRLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMHSIWLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFAGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNRDGLQRALLQLEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            3545..4069
                     /gene="ssb"
                     /locus_tag="ACSIGJ_001784"
     CDS             3545..4069
                     /gene="ssb"
                     /locus_tag="ACSIGJ_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HHQ1330598.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYDEGYGQNQEAYQRPAQQSRQPAPDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
     gene            4442..5365
                     /locus_tag="ACSIGJ_001785"
     CDS             4442..5365
                     /locus_tag="ACSIGJ_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HHQ1330599.1"
                     /translation="MENQRPLLGFALALLAAMTWGMLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWHSFRLLLLGVTGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLVGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFVYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALVVVGGAVTAAVGDRLFKRR"
     gene            complement(5430..6818)
                     /gene="fumC"
                     /locus_tag="ACSIGJ_001786"
     CDS             complement(5430..6818)
                     /gene="fumC"
                     /locus_tag="ACSIGJ_001786"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HHQ1330600.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYRPVHPNDHVNRAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHSLGRLNDAL
                     KGLYELALGGTAVGTGLNSHPEYAEIAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNENCAAGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNDKSLRETAVGLGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(6962..8941)
                     /gene="tkt"
                     /locus_tag="ACSIGJ_001787"
     CDS             complement(6962..8941)
                     /gene="tkt"
                     /locus_tag="ACSIGJ_001787"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HHQ1330601.1"
                     /translation="MSQLANVIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQTAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     AYPAFEIPQEIYDAWNAKEKGAKLEAGWNELFAQYQAKYPAEAAEFVRRMDKKLPENF
                     DEYVQTALKEVCAKAETVATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAAKAED
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGAYVISEAQGNAQAVIIATGSEVGLAVEAQK
                     VLAGQGIAVRVVSMPSTSVFDRQDAAYQAAVLPEGLPRIAVEAGHTNGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
BASE COUNT         1825 a         2275 c         2707 g         2198 t
ORIGIN      
        1 gcaagcgtag gtcggatact tgtatccgac aaaaccttta acattcccat cattgcaatc
       61 cattgcagca atgtccaaaa tgtcgaattc aagaatccgg cctacaaaat cattccgagc
      121 ataatactat gaaataccgt cgtttttacc gcaatggcgg cacttacttt tttacggttg
      181 taaccaataa acggcagaag attttgaccg atgatgcggt gcgtttggct ttacggcagg
      241 cggtaatggc ggtgcgcgaa cggaatccgt ttgaaatttt ggcatgggtg ttgatgcccg
      301 atcatctgca taccatatgg cggctgccgg acaatgattc tgcttattcg gaacgctggc
      361 ggcaaatcaa gcggcacagc caatatttaa tcggcggcaa tctcaggctt tggcaaaacg
      421 cttttgggaa catactattc gcggtaaggc cgattttgcc tgccattttg attatctgca
      481 tttcaatccg gtcaaacatg gctatgtagg acaaatttcc gattggcggt tttctacgtt
      541 tcaccgttat gtcaaacagg gtatttatcc gcataattgg ggtggcggca atgcggactt
      601 ttctattgaa tacgattgaa gtaatgtcgg attcgagaat ccgacctacg gaaaactgaa
      661 agagcatcgg ctgctggacg gcattatgcg caaagcccgc cgcaaccgcc cgctgacgga
      721 ggcgcaaacc aaacgcaacc gatatttgtc gaagacccgt tatgtggtcg aacaaagctt
      781 cggtacgctg caccgtaagt tccgctatgc gcgggcagcc tatttcggac tgattaaagt
      841 gagtgcgcaa agccacctga aggcgatgtg tttgaacctg ttgaaagcgg ccaacaggct
      901 aagtgcgccc gctgccgcct aaaaggcggc ccggatgcct gattatcggg tattcaaggg
      961 ggattaaggg ggtatttggg tagagttagg agtgattggt ggcggaaaca gccgaaaatc
     1021 tgtgtcaggg tttcagctgt cgggagggaa gggaattttg caaaggtctc aagatatagt
     1081 ggattaaatt taaaccggta cggcaaggcg aggcaacgcc gtaccggttt aaatttaatc
     1141 cactatattt ccgcctgctg ccgcccgaaa agcgtaatgc gctgatggtg ctgctgcaca
     1201 atccggacgg cggataaccg gtgaaaacgg gaaatgcccg ggcggtgtcg tctgtgcctg
     1261 tttggacaag cgttcttgag ggcggtaaaa tcaaggtttg cccgaaaagg gcagggcgat
     1321 atgccgcgct tggattttgc gcgcggtttt tcgcgttgga agcaatcatg tctgtttgaa
     1381 tatttaatgc cgtctgaaac gtccggacgg acccccgttc cttatgaaaa aaccgaccga
     1441 taccctaccc gtcaatctgc aacgccgccg cctgctgtgt gccgccggcg cgctgttgat
     1501 cagcccgctg gcgcacgccg gcgcgcaacg tgaagaaacg cttgccgacg atgtggcttc
     1561 cgtgatgagg agttctgtcg gcagcgtcaa tccgccgagg ctggtgttcg acaatccgaa
     1621 agagggcgaa cgttggttgt ccgcgatgtc ggcacgtttg gcaagattcg tccccgacga
     1681 gggggagcgg cgcaggctgc tggtcaatat ccaatacgaa agcagccggg ccggtttgga
     1741 tacgcagatt gtgttggggc tgattgaagt ggaaagcgcg ttccggcagt atgcgataag
     1801 cggggtcggc gcgcgcggtc tgatgcaggt tatgccgttt tggaaaaact acatcggcaa
     1861 accggcgcac aacctgttcg atatccgtac caacctgcgt tacggctgca ccatcctgcg
     1921 ccattaccga aaccttgaaa agggcgacat cgtccgcgca cttgcccgct tcaacggcag
     1981 cctcggcagc aataaatatc cgaacgccgt tttgggcgcg tggcgcaacc gctggcagtg
     2041 gcgttgattt tgaacccgcg ccgcaaccga aatacggcga atcctgtata atccgaaaat
     2101 ctgttcactg gaagttcaga cggcattgca actgttgatg ccgtctgaaa aattatttat
     2161 ggcaagagac aaccgcatcc aaatgtttcc gcacgaatgg cgcgccagta cgacgctttc
     2221 cggcgtgtac gcgctgcgta tgctgggtat gttcctcgtg ctgcccgttt tggcaatata
     2281 tgccgcctcg ctgcccggtg cggaagacaa taaaacgctg gtcgggctgg caatgggcat
     2341 ttacgggctg acgcaggcat tgctgcaact gcctttgggt atcgcttccg acaagttcgg
     2401 gcgcaagaaa accatttatg tcgggctggt cgtgtttgcc gccggcagct tccttgccgc
     2461 cgccgccgat acgctgccca tgctggttgc cgcgcgcgcc atacagggcg cgggggcggt
     2521 cagtgctgcc gtaaccgcgc tgcttgccga cttgacgcgc gacggcgtgc gtacccgcgc
     2581 gatggcgatg atcggtttga gtatcggtct gacgttttcg gtcagcctgg tggttgcgcc
     2641 gatgattgcc gatgtggccg gcgttcgcgg actgtttatg ctgaccggca ttctgaccgc
     2701 catcagcatc ggcgtagtgg cgtggatgac tcctgatccc gaagtttcca agctgcatga
     2761 ggacacgcag gcgcagcctt cgcgcatagg agaagttttg aaaaaccgta ggctgctgac
     2821 gctcgatttc ggcattttcg ccctgcacgc cgcgcaaatg gcattgttta ccgcgctgcc
     2881 tttcgcgatg acccggctcg gtttggaaaa aatccagcat tggaaagtat atctgccctc
     2941 gaccattacg ggcttggtgg tgatggttcc gctgattatc gtcggcgaaa cgcgcaacaa
     3001 gctcaaacag gtttttgttt tgggcatcgt ctgcatcgcg gcggcgcagc ttggtttgct
     3061 gtccggaatg cactcgattt ggctgattac cgcttatctg gttgtttact ttatcggttt
     3121 taatgtgttg gaagcgagcc tgccgtctat ggtttccaaa atcgcgccgt ccgacctgaa
     3181 gggtacggcg atgggcgtgt acaacacgat gcagtcgctc ggactgtttg ccggcggcgc
     3241 ggcaggaggc ttgctgtttc aaaaatacgg ctttgccggc gtgtttgcct tttgcagcat
     3301 attgatgctg ctgtggctgg tgattgccgt tttatcgccc gcgcccaagc ccgtcaaaaa
     3361 cctcagttac cctgtcggcg gcgtgtggca gggcaatcgg gacggtttgc aacgcgccct
     3421 gttgcagttg gaaggcgtgg aagacatcgg tttcagtttc gacgggcaga ccgtctatct
     3481 taaagtgttg cagaagggtt tcgatcaggc tgccgctgaa aaaatcatta caggagttta
     3541 aaaaatgtca ttgaacaaag tcatccttat cggccgcctc ggacgcgatc ccgaagtgcg
     3601 ctatatgccc aacggcgagg cagtgtgtaa tttcagcgtc gccaccagcg aaacttggaa
     3661 cgaccgcaac ggccagcgcg tagagcgcac cgaatggcac aacatcacga tgtaccgcaa
     3721 actggcggaa attgccgggc aatacctcaa aaaaggcggg ctggtttatc tggaaggcag
     3781 aatccaaagc cgcaaatacc aaggcaaaga cggcatcgaa cgcaccgctt acgatattgt
     3841 cgccaatgaa atgaaaatgt tgggcgggcg caatgaaaac agcggcggcg cgccttacga
     3901 tgaaggttac ggtcagaatc aggaggctta ccaacgcccc gctcagcaaa gccggcagcc
     3961 cgctcccgac gcgccgtccc atccccaaga agcacccgcc gcgccgcgcc gccaacccgt
     4021 gccggccgcc gccccggtcg aggacattga cgacgacatc ccgttctgaa ttttacggcc
     4081 ggacatcctc gcggagggaa gtcataaagg acggaaaaac cttaaacctt acggggcaag
     4141 gtttctcttt tttgcatttg ggccggcttg ttgccgcccc ctgcgttggg cgtaacgttg
     4201 cgattgcgcc gttcgggcgt tttgaggcaa gcgatgcctg ccgcactccc ccgctgtttt
     4261 cagacggcat tttaaaattg aggcggtgtt ttcaaatagt gcgaagcaat agtcgggctt
     4321 cagccagttt gtttgaaatc aaagggttgg aatcaaacgc caaacgcttg aaccatcatt
     4381 gcccgatgcg gcaaaccggc atcgtccggc aatacggttg tttaaaaata gggaaatcag
     4441 gatggaaaac caaaggccgc tcctaggctt cgcgttggca cttttggcgg cgatgacgtg
     4501 ggggatgctg ccgattgccg tgcggcaggt attgaagttt gtcgatgcgc cgacgctggt
     4561 gtgggtgcgt tttacagtgg cggcggcggt attgtttgtt ttgctggcat tgggcgggcg
     4621 gctgccgaag cggcgggatt tttcttggca ttcattcagg ctgctgctgc tcggcgtgac
     4681 gggcatttcg gcaaactttg tgctgattgc ccaagggctg cattatattt cgccgaccac
     4741 gacgcaggtt ttgtggcaga tttcgccgtt tacgatgatt gttgtcggcg tgttggtgtt
     4801 taaagaccgg atgactgccg cgcagaaaat cggtttggtt ttgctgcttg tcggtttgct
     4861 tatgtttttt aacgacaaat tcggcgagtt gtcgggtttg ggcgcgtatg cgaagggcgt
     4921 gttgctgtgt gcggcaggca gtatggcctg ggtgtgttat gccgtggcgc aaaagctgct
     4981 gtcggcgcaa ttcgggccgc aacagattct gctgttgatt tatgcggcaa gtgccgccgt
     5041 gttcctgccg tttgccgaac cggcacacat cggaagtttg gacggtacgt tggcgtgggt
     5101 ttgttttgtg tattgctgct tgaatacgtt aatcggttac ggctcgttcg gcgaggcgtt
     5161 gaaacattgg gaggcttcca aagtcagcgc ggtaacaacc ttgctccccg tgtttaccgt
     5221 aatattttct ttgctcgggc attatgtgat gcctgatact tttgccgcgc cggatatgaa
     5281 cggtttgggt tatgtcggcg cactcgtcgt ggtcgggggt gcggttacgg cggcggtggg
     5341 ggacaggctg ttcaaacgcc gctagttcgc aggcaacgga aaatgccgtc tgaacgaggc
     5401 ttcagacggc attttatttg agggaaggat tagcgcggat gaaccatatc ggcagggacg
     5461 accagttcgt caaactcttc gcccgtcagc aagcccaacc caacggcagt ttcgcgcagc
     5521 gatttatcgt ttttgtaggc ggttttggcg actttggcgg cgttttcgta gccgattttg
     5581 cggtttaacg cagtaaccag catcagggaa tggtgcagga aatagtcgat tttttccggt
     5641 acgggttcga tgccggcggc gcagttttcg ttgaagctgt tgcacgcgtc gcccaagagg
     5701 cggatggatt gcaagaggtt gtaggcgata acgggcatat agacgttcag ctcgaaattg
     5761 cccgacgcgc ccgccatacc gatggtaacg tcgttgccga acacttggca gcacaccatc
     5821 gtcatcgctt cgcattgggt cgggttgact ttgcccggca taatggacga acccggttcg
     5881 ttttcgggga ttttgatttc gcccaagccg cagcgcgggc cgcttgccag ccagcggatg
     5941 tcgttggcga ttttgttcag gcttgccgcc agcgttttca atgcgcccga agcggcaacg
     6001 gcggcatcgc gtccgcccaa ggcttcaaat ttgttcggcg cgctgacaaa cggcaggccg
     6061 gacagttcgg cgagtttggc ggcggctatt tcagcgtatt cgggatggct gttcaaaccc
     6121 gtaccgaccg ccgttccacc caaggcgagt tcgtacaaac ctttgagcgc gtcgttcaga
     6181 cggcctaagc tgtggtcgag ttgggaaacg tagccggaaa attcttgtcc caaagtcagc
     6241 ggcgtcgcat cctgcaagtg ggtacggccg attttgacga tgggggcgaa ggcttgggct
     6301 tttttgtcca atgtatcgcg caaggctttt accgccggaa taaggtggcg gttgatttca
     6361 atcgcggcgg cgacgtggat ggcggtcggg aacgcgtcgt tggtcgattg cgcgcggttc
     6421 acatggtcgt tggggtggac gggccggtac gccgccaaac ccgtacctgc aatttcgttg
     6481 gcgcggtttg ccagcacttc gttcatgttc atattggact gcgtaccgga accggtctgc
     6541 caaaccacta aggggaactg cccgtcgagc ctgccgttca gtacatcatc cgccgcctgc
     6601 gtaatcaaat ccgcctgttc gggcttaatc ctgccgaggg aaacattggt cgcggcggcg
     6661 gcttttttca ccaacgccaa agcgtaaatc agcggctgcg gcagggtttc gccgccgatt
     6721 ttgaaattgt tgcggctgcg ctgggtctgc gcgccccaat aggcttcgga cgggacttcg
     6781 acattgccca tcgtgtcgtg ttcggtgcgg gtgttcatac gtttctcctt ttgaaatgtg
     6841 aataagagtg attcgcaaat attataatgg agattggcgg aatgaggaag catcaaggcg
     6901 ggagggcaaa aaaatgccgt ctgaaaagca ttcagacggc atttgatgcg gcggctgagg
     6961 tttacagcac ggatttcacc gtatcaacca cattgtccac ggtaaagccg aatgctttga
     7021 acagcagatc ggcaggggcg gattcgccga agcggttgat gccgacgact gcgccgttca
     7081 gtccgacata tttgtaccag ccgttggtgt gtccggcttc tacggcgatg cgcggcaggc
     7141 cttccggtag gacggcggct tgataggcgg cgtcttggcg gtcgaagacg ctggtggacg
     7201 gcatggaaac gacgcgcacg gcaatacctt gtcctgccaa tactttttgc gcttctacag
     7261 ccaatcctac ctcggaaccg gtggcaatga tgacggcttg ggcgttgcct tgggcttcac
     7321 tgattacgta agcaccgcgt ttgatgtcgt tcagttgttg ctcgctgcgt gcttggaatt
     7381 tcaggttttg acggctgaaa atcaggcagg acgggtggtc ttcggctttt gccgcttctg
     7441 cccaagcgac cagagattct gcggtgtcgc acggccgcca tacgtccata ttcggaatca
     7501 ggcgcagggt ggcggtttgc tcgataggct ggtgggttgg gccgtcctcg cccaaaccga
     7561 tggaatcgtg ggtgaatacg aagacggggt tgattttcat cagtgccgcc atgcgtaagg
     7621 cgttgcgttc gtattcgctg aacatcagga aagtcgcgcc gaagggtttt acaccgccgt
     7681 gcaataccaa accgttcata atcgcaccca taccgaactc gcgcacgccg tagtggatgt
     7741 agttgccgcc tttgtcgcgg gtaacggaga cgctgtttga ccagtcggtc aggttggacg
     7801 gggtcaggtc ggcagaaccg cccaccagtt cgggcagctc ttttgccaag atttcgatgc
     7861 tgttttggct ggctttgcgg gtggcgacgg tttccgcttt ggcgcacact tctttcaatg
     7921 ccgtctgaac gtactcgtca aagttttccg gcagcttttt atccatgcgg cgcacgaatt
     7981 ctgcggcttc ggcaggatat ttggcttgat attgcgcgaa cagttcgttc catccggctt
     8041 ccagtttcgc gcctttttct ttggcattcc acgcatcgta aatttcttgc gggatttcaa
     8101 acgcaggata agcccaaccc aaatgtttgc gcgtggcttc gatttcgtcc gcacccaaag
     8161 gcgcgccgtg ggttttgtgg ctgccttctt tgttggcgct gcctttgccg attaaggttt
     8221 tgcagcagat gatggacggc ttgccggttt cggcgcgggc ggcttcgatg gccgtctgaa
     8281 tggcggcggt gtcgtggccg tttacgttgg gaacgacgtg ccagccgtag ctttcaaagc
     8341 gttgcgggat gttttcggta aaccagccgt ccactttgcc gtcgatggag atattgttat
     8401 cgtcatacaa aacaatcagt ttgcccaagc ccaaggtgcc ggcgagtgag caggcttcgt
     8461 gcgatacgcc ttccatcagg cagccgtcgc ccataaagac gtaagtgtag tgatcgacga
     8521 tgttcaaacc gtctttatta aattcggcgg caaggatttt ttctgccaat gccatgccca
     8581 ctgcgttggc aataccttgc cccaacgggc cggtcgtggt ttccacgccg tcggtgtagc
     8641 cgtattcggg atggccgggg gttttgctgt gcagctggcg gaagtttttc agatcttcaa
     8701 tgctcaggtt gtagccggtc aggtgcagca ggctgtacaa cagcatagac gcatgaccgt
     8761 tggagaggac gaagcggtcg cggttgtaga atttgggatt ggcgggattg tgattgagga
     8821 atttcgtcca caatgtttcc gccatttccg ccatacccat aggcgcgccg gggtggccgg
     8881 aattggcttt ttgaacggca tcggccgaga ggaagcggat tacgtttgcc agttgagaca
     8941 ttttgtattt tccttgctgg tgtttcggat aagtggataa tcggaaggcg ttgattatcg
     9001 cccga
//
LOCUS       DBJGGI010000073         8839 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.073, whole genome shotgun sequence.
ACCESSION   DBJGGI010000073 DBJGGI010000000
VERSION     DBJGGI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8839
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.073"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(6..1460)
                     /gene="mgtE"
                     /locus_tag="ACSIGJ_001788"
     CDS             complement(6..1460)
                     /gene="mgtE"
                     /locus_tag="ACSIGJ_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HHQ1330602.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLAELHPADVADVLESLPPRERNIVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVCIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITIDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMVAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLSLATLFLL"
     gene            complement(1694..2446)
                     /gene="gloB"
                     /locus_tag="ACSIGJ_001789"
     CDS             complement(1694..2446)
                     /gene="gloB"
                     /locus_tag="ACSIGJ_001789"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HHQ1330603.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTRFTFGNGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTVEQLYDSFQRFNQLPEGT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAERTPTLPVTLAHERRVNPFLRTEIP
                     AVRQRAEALVGKTLNSGLEVFAALRELKNAYR"
     gene            2678..2989
                     /locus_tag="ACSIGJ_001790"
     CDS             2678..2989
                     /locus_tag="ACSIGJ_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HHQ1330604.1"
                     /translation="MPQQTMNLMRECIPIFTVLSDENRHQILHVLWKHGRMNVNELTE
                     HLHLSRPAVSHHLKIMLQAGAVAVEQVGKERFYSIAMADAVARLKQLADLMAQNCPLS
                     K"
     gene            3063..4274
                     /locus_tag="ACSIGJ_001791"
     CDS             3063..4274
                     /locus_tag="ACSIGJ_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007241529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HHQ1330605.1"
                     /translation="MLFTPFTFQNGKTAKNRIFKSAMEEQLAQNDQPSEKLVRLYGAW
                     AEGGAGVLVTGNVMVAESGKGSINDVLISDDRALEMLKKWAKARMQNDTLLIMQINHA
                     GKQSPAVVNKTPLAPSAVPLVGMNGFINPPRELSADEINGLIQQFVQTAKIAEQAGFS
                     GVQIYAVHGYLISQFLSPHHNRRQDQWGGSLENRMRFLLETYTAIRAAAGKDFLVGVK
                     LNSADFQKGGFDESESVQVVQKLSEMGIDFIEVSGGNYESPQMLAAKDSTRKREAFFI
                     DYAEKARAASQAPLIITGGFRSQTAMEDALSSGHLDLVGIARPFALVPDLANKMQNRT
                     YQTVQADRIQTGVAFVDKKAGAMLEMNWYMTQMDLIGQGKQSNPKLSAWKVLLKTLRE
                     NGKAGLSTGRA"
     gene            complement(4345..8301)
                     /gene="purL"
                     /locus_tag="ACSIGJ_001792"
     CDS             complement(4345..8301)
                     /gene="purL"
                     /locus_tag="ACSIGJ_001792"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HHQ1330606.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPEAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLKSET
                     FSTVDVLGGGKEALVKANTETGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIKGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDQNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFELREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFSNNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHAGDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPAVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVKDVRKTVTPELKNVEGSVLLFIDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDAGRLKAFYSVIQQLVAEDKLLAYHDRSDGGLFATLAEMAFAARCGISADIDCLMD
                     KFLPIHLPDFQGDPAEDLSDELYNHAAIKILFNEELGAVIQIRQKDRDYVDAAFETAG
                     LTDAVSRIGSPDFDNEFISFFGYGYFLEQNRADLQRAWQETSHAIQRLRDNPACADSE
                     FALIGDNERSALFADVKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFTRA
                     GFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQFA
                     AFFADPNTLTLGVCNGCQMVSNLAEIIPGAETWPKFKRNLSEQFEARLNMVHVPKSAS
                     LILNEMQDSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNPNG
                     SPQGIAGITNADGRVTIMMPHPERVYRAAQMSRKPEGWTELSGWYRLFAGARKALG"
     gene            8452..8790
                     /locus_tag="ACSIGJ_001793"
     CDS             8452..8790
                     /locus_tag="ACSIGJ_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HHQ1330607.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERSDAAV"
BASE COUNT         1937 a         2305 c         2557 g         2040 t
ORIGIN      
        1 ggcatttaaa gcaggaacag cgtggcgaga ctcaagaaaa tcaggaagcc gccggagtcg
       61 gtaacggcgg taatcagcac cgagctgccc agcgcgggat cgcgtccgaa tttttccatt
      121 accacgggaa tcaatacgcc gaccgttgcc gccagcagga ggttgagcgt catcgcggca
      181 accatgacca aaccgatgcc gaggctgccg taaagcagcc acgaaaccgc tcccatcacc
      241 gtcccccaaa taatgccgtt gaccaaggcg acgccgactt cttttttcaa cagccttccc
      301 gcctgcatat ccgtcagctg ccccatcgcc atcgcgcgga caatcatggt aatcgtctgg
      361 ttgcccgagt taccgccgat gccggcgacg atgggcatca gcgcggcgag tgcgacgatt
      421 ttttcgatac tgccttcaaa cgcgccgatg acgcggctgg cgaggaaggc ggtgcagagg
      481 ttgacggcga gccacatcca gcggtttttg acggaatcca agacgggggc gaacaggtct
      541 tcctcttcct gcaaacctgc catgttcaac atatccgctt cggattcttc gcggatcacg
      601 tccaccatct cgtcgatggt aatcctgccg atgagctttt tgttttcatc aacgacgggc
      661 gcggtaacca agtcgtagcg ttcaaacgcc tgcgccgctt cttccacgtc gtcctcggcg
      721 cggaaacgca cgacatcttt cgccatcacg ttttccacca aatcttcggg atcggcgacc
      781 aaaagtttgc ggatgggcag cacgccctgc agcacgtcgt tttcatcgac gacaaaaatc
      841 ttgtcggtat ggtcgggcag gctgtcgaag cggcgcagat aacgcagcac cacttcgcag
      901 gcgacatcgg cgcggatgca gaccaactcg aagtccataa tcgcaccgac ttggttgtct
      961 tcatacgaca ttgccgcctt gacttgggcg cgctcttcct catcgcgcgt ctgtaaggct
     1021 tcataaacca cttggtgcgg caaatcgtct gccagctccg ccaattcgtc cgcatccaaa
     1081 tcatcgaccg ccgccaacaa ttcgtccttg tccattgact cgatcagcgt ttctcgcacc
     1141 gcgtcggata cttccagcaa tacttcgccg tcgtcttcgg gtttgaccag aagccaaacg
     1201 atattgcgtt cgcgcggcgg cagggactcc aagacgtcgg ccacgtcggc agggtgcaac
     1261 tcggccaaga ggacggtcag ctcggtcagt ttgtcgcgca gcggcgcatc ttcgagcggt
     1321 gtgccgtttt cgatttgttc aaaagcaggt tcgaggattt cgcagaggga gtggacacgg
     1381 tcgaaatcgg cggaagggcg ttcggcatgg ttttgaccgg tttcgccgtc gtgtgtcgga
     1441 ggagtgtgtt cgatgctcat aaatgctccg cccgccgtgt gcggggaggc attccggcgg
     1501 gatggttatt gtcgtttgga atcggaaggg gtgttcagta aaaacgaact gggaaggtcc
     1561 ataataaaag cccgaaaaat caggcacggg attggaaaca gcactattct actccctttt
     1621 gaaacggttt actattttta acgcaaatgc cgtctgaaac gcgggtttca gacggcattt
     1681 ttcggagggc aggtcagcgg taggcgtttt tcagttcgcg cagggcggcg aatacttcca
     1741 aaccgctgtt cagcgttttg ccgaccaggg cttcggcacg ttggcggacg gcggggattt
     1801 ctgtccgtaa gaacgggttg acgcggcgtt cgtgcgcgag ggtaacgggc agggtgggcg
     1861 tgcgttccgc cgccttcaat gccgtctgaa tgtcggcgtt gtccggctcg atatgggcgg
     1921 cgaaacgcag gttggcggcg gtgtattcgt gtgccggata gaacagggtg ccttcgggta
     1981 attggttgaa ccgttggaag ctgtcgtaaa gctgttcgac cgtgccggta aacacgcgtc
     2041 cgcagccggc ggaaaaaagg gtgtcgccgc agaaaacgtg tatgccgtct gaagtttcga
     2101 gaaggtagct ggtgtggcgg tctgtgtggc cgggtgttgc ccaaacggta acctgaccgt
     2161 tgccgaaggt gaatcgtgtg ccggcggtta cggtgtgggt ggcggcttcg atgtcggatt
     2221 cgccgtaaac gggcgattcc atgtagccgc gccagagtgc cgccgcaccg ccttcgtggt
     2281 cgggatgggg gtgcgttacc caggtttggg cgagcatgag tcggttgcgt acgaggaatt
     2341 ccaagacggg cgagggttcg gaaggatcga cgcagacggc atggttgccg tgctgtatca
     2401 tccagatgta gttgtcggtc agggctttga cgggggtaat tttcatgagt gttccttgat
     2461 ggttcggatg ccgtctgaaa ggtgtatccg aaaggcgtta atatacagcg gaaccctagc
     2521 aaacgccatc atgctgtttc aggcggcatt ttttcttata aaaacctttc agcaacaata
     2581 tattgccaaa ctgcaaacca aactgccttt gataactgcc gaccttccct ctaaaatacg
     2641 ttcgaatttt taaatatata aactcaatag atcgaatatg ccgcaacaaa ccatgaattt
     2701 aatgcgcgag tgcataccca tttttaccgt attgagcgat gaaaaccgcc atcagatcct
     2761 gcatgtattg tggaaacacg gtaggatgaa tgtgaatgag ctgaccgagc atctgcatct
     2821 gtcgcgccct gctgtgtcgc accatttaaa aatcatgctg caagccggag cggtggcggt
     2881 ggagcaggtc ggcaaagagc ggttttacag tattgccatg gcggatgcgg tggcgagatt
     2941 gaaacagctt gccgatttga tggctcaaaa ttgcccgctt tcaaaatagg gttatccgtt
     3001 ggccctttat ttttacccaa atacgtttac atttttaaac ttatcaactt aaaggaacga
     3061 tcatgttatt cacgccattc acttttcaaa acggcaaaac cgccaaaaac cgtatcttca
     3121 aatccgccat ggaagagcaa cttgcccaaa acgaccagcc gtctgaaaaa cttgtccgcc
     3181 tgtacggcgc ttgggcagaa ggcggcgcag gcgttttggt gacgggcaat gtgatggtgg
     3241 ctgaaagcgg caaaggttcg ataaacgatg tgttgatttc agacgaccgc gcccttgaaa
     3301 tgctgaaaaa atgggcgaaa gcccgcatgc aaaacgacac gctgctcatc atgcaaatca
     3361 atcatgcggg caaacagtcg cctgcggtgg tcaataaaac accgcttgca ccgagcgccg
     3421 tgccgctggt gggcatgaat ggctttatca atccgccgcg cgaattgagt gccgatgaaa
     3481 tcaacgggct gattcagcaa ttcgtacaaa ctgccaaaat tgccgaacag gcaggctttt
     3541 caggcgtaca aatttacgcc gtacacggtt atctcatcag ccaattcctc tcgccgcacc
     3601 acaaccgccg ccaagaccaa tggggcggca gtttggaaaa ccgtatgcgc ttccttttgg
     3661 aaacctacac cgctattcgc gctgccgcgg gcaaagattt cttggtgggc gtaaaactca
     3721 attcggcaga tttccaaaaa ggcggatttg acgaaagcga atcggtgcag gtggtgcaaa
     3781 aactgtcgga aatgggcatt gattttattg aagtttccgg tggcaactac gaaagcccgc
     3841 aaatgctcgc cgccaaagac agcacccgca aacgcgaagc cttcttcatc gattacgccg
     3901 aaaaagcccg tgcagccagc caagccccgc tgattatcac cggcggattc cgttcgcaaa
     3961 ccgccatgga agatgcctta tccagcggtc atttggattt ggtcggcata gcccgcccgt
     4021 ttgccttagt ccctgatttg gcgaacaaaa tgcaaaacag aacttaccaa accgtacaag
     4081 cagaccgcat ccaaactggc gtagcatttg ttgataaaaa agcgggtgca atgttagaaa
     4141 tgaactggta tatgacgcaa atggatttga tcggacaagg aaaacagtcc aaccctaaat
     4201 tgtcggcgtg gaaagtattg ctgaaaactt tgcgggaaaa cggtaaagcg ggattgagta
     4261 ccggcagggc ttgagaatgg tcaaaatgcc gtctgaaacg ggtttcaaaa cctttttcag
     4321 acggcatttg tttgggcaga cggtttagcc caaggcttta cgtgcgccgg caaagaggcg
     4381 gtaccagccg gacagttccg tccagccttc cggtttccgg ctcatttgtg cggcacggta
     4441 cacgcgttcg gggtggggca tcataatggt aacgcggccg tcggcgttgg taatgccggc
     4501 gatgccctgc ggcgagccgt tggggttgag cggataggtt tgggtaacct ggttttgacc
     4561 gtccacatat tgcagcgcga tgcccaaatc ggcggaaata ttgccgccgt gaagcgcgaa
     4621 gtcggcgcgg ccttcgccgt ggctgaccac gacaggcagg ctggagtctt gcatttcgtt
     4681 caaaatcagc gacgctgatt tggggacgtg aaccatattc aggcgcgctt caaactgctc
     4741 gctcaggttg cgtttgaact tcggccatgt ttctgcgccc gggataattt cggcaaggtt
     4801 gctgaccatt tggcagccgt tgcacacgcc caatgtcagc gtgttcggat cggcaaagaa
     4861 ggcggcgaat tggtcgcgca aggcagggtg gaacaggatg gatttcgccc agccttcacc
     4921 cgcgccgagt acgtcgccgt agctgaagcc gccgcacgcc gccagcattt tgaagtcggc
     4981 aaggcggaag cggccagcca tcaggtcgga catatgcacg tcgtaggcat cgaatccggc
     5041 gcgggtgaag gcggcggcca tttcgatttg cccgtttacg ccctgttcgc gcaggatggc
     5101 gattttaggt ttcgcgccac tgttgataaa cggcgcggcg atgtcttcgt tcacgtcgaa
     5161 cttcacgtcg gcaaacaatg cgctgcgttc gttgtcgcca atcagggcga actcgctgtc
     5221 ggcgcaggca gggttgtcgc gcaggcgttg gatggcgtgg ctggtttcct gccacgcgcg
     5281 ttgcaggtcg gcgcggtttt gttccaagaa ataaccgtaa ccaaagaaag aaataaactc
     5341 attgtcaaaa tcaggagagc caatccggct gaccgcatcg gttaagccgg ccgtttcaaa
     5401 tgccgcatca acataatccc tatctttttg gcggatttgg ataaccgcgc ctaattcttc
     5461 attgaataag attttaatgg cggcatgatt ataaagttcg tcagataagt cttcggcagg
     5521 gtcgccttga aaatccggga gatgaatcgg caggaattta tccatcaggc aatctatatc
     5581 ggcactaata ccgcaccgtg ccgcaaacgc catttccgcc agcgtcgcaa acaaaccgcc
     5641 gtcgctgcgg tcgtgatacg ccaaaagttt gtcttcggcg acaagctgtt gaattacgct
     5701 gtaaaacgct ttcagacggc ctgcatcgtc caaatcgggc gcgtcgccgc tcatgttgtt
     5761 gtacacctga ccgaacgccg agccgcccat gcgcgctttg ccgaagccca aatcgataaa
     5821 cagcaatacg ctgccttcga cgtttttcaa ttcgggcgta acggtttttc gcacgtcttt
     5881 aacaggcgcg aacgcggaaa taatcaggct caacggcgaa accacggatt ttttctcttc
     5941 gccgtcctgc cacacggttt tcatcgacag gctgtcttta cctacgggaa tgctcaaatc
     6001 caatgcctgg caggctttag aaacggcttc gacggtgcgg tagagttttt cgtcttcgcc
     6061 ttcgttgccg cacgccgcca tccagttggc ggaaagtttg atgttgccga tgtcgccgat
     6121 gttgactgcc gcgatgttgg tgatggcttc gccgacgcac attctgcccg aggcgggcgc
     6181 gtcaaacagg gcgacggccg gtttttcgcc catagacatc gcttcgccgc gataggtgtt
     6241 gaagcccatc atggtaacgg cgcagtcggc tacgggggtt tggtatttgc cgaccatttg
     6301 gtcgcggtgg gtcatgccgc cgacgctgcg gtcgccgatg gtaatcagga agtttttggc
     6361 ggctacggca ggcaggcgca aaacgcggta ggcggcttcg gtgatgtcga tatcgcccgc
     6421 gtgaaacggt tttttggacg gcgtaaccgt tttgtcggta cgcgtggttt tgggcggttt
     6481 gccgagcaag acgttcagcg gcaaatcgac ggggttgttg gagaacaaat cgtcgcgcac
     6541 tttcaaatga ccgtcgtcgg tcgccgtgcc gactacggca aacgggcagc gttcgcgttc
     6601 gcagatggcg cggaaggtgt ccaaatcttt ttccaaaatc gacaagacat aacgctcttg
     6661 cgattcgtta caccagattt gcaaaggcgt taaaccgtgt tcttccaacg gcacttcgcg
     6721 cagctcgaat accgcgccgc gtccggcatc gttgaccaat tcagggaaag cgttggacaa
     6781 gccgcccgcg cccacgtcgt ggatggagat aatcgggttt tggtcgccga gctgccagca
     6841 gcggtcgatg acttcctgcg cgcggcgttc gatttcaggg ttgccgcgtt gtacggagtt
     6901 gaagtccaaa gacgcatcat ttgtaccggt atccatcgaa gaagccgcac cgccgcccaa
     6961 gccgatgagc atgcccgggc cgcccagttg gatcagcagt gcgccttcgg ggatttcgtc
     7021 tttgtgcgtc tgctgcgcct gaatgctgcc caaaccgccg gcaatcataa tcggtttgtg
     7081 gtagccgcga acctgaccgt caaatttttc ttcaaaggtg cggaagtagc ccaagaggtt
     7141 cgggcggccg aattcgttgt tgaacgccgc accgccaatc gggccttcaa tcatgatgtc
     7201 caacggcgag gaaatgtgtt ccggcttgcc gtagtcttgt tcccacggct gtttgaggtc
     7261 ggggatgttg aggttggaca cggtaaagcc ggtcaagccc gctttcggac gcgaaccttt
     7321 gcccgtcgca ccttcgtcgc ggatttcgcc gcccgcgcct gttgccgcgc ccgcaaacgg
     7381 cgcgatggcg gtcgggtggt tgtgcgtttc cactttcatg atgatgtggg tgtcttcctc
     7441 gtggaaacgg tagccttggt tttccgctgc attcggatag aaacgctcga ttttcgcgcc
     7501 tttgatcacg gacgaattgt ctttataggc gacgaccgta ccttcgggat gcgcgttgtg
     7561 cgtgtcgcgt atcatgccga acagggattt cggttgtttt tcgccgttga ggatgaaatc
     7621 ggcgttgaag attttgtggc ggcagtgttc gctgtttgcc tgcgcgaaca tcatcagctc
     7681 cacatcggac ggattgcgct gcaaagcctg atagttttca accagataat cgatttcgtc
     7741 ggcggagagt gccaaacccg tttcggtatt ggctttgacc aaagcctctt taccgccgcc
     7801 caaaacatcg acggtggaga aggtttcgga tttgagatgg tggaataatt tggatgccgt
     7861 ctgaaaatcg ggcagcacgg attcggtcat gcggtcgtgc agcaacgccg cccattgctg
     7921 tttctgttca tcggtaagcg caccttccag ccacaccgcc ataccgcgct cgatgcgttc
     7981 gatgcctgcc aaaccgcagt tttccgcgat attggtcgcc ttggaagccc acggcgaaat
     8041 cgtacccaaa cggggcgtta ccaaaaacaa atgcaaaccc tcgcgcgctt cgggcgtttg
     8101 ttcaacgctt tgcgccgcca acaaggcttg cagtttttcg acagtcgcgg catcaagtgc
     8161 tttctcgctg ccggcgaaat accaaaattc gctgcttaat ttgacttcgg gcaggccgag
     8221 tgcggcggct ttttgcaaga gtttttcgac acggaaatcg gaaagggcgg taacgccgcg
     8281 caagggcaaa acaacagaca tggattcggc tctcaaatgc ggttggaaaa gctgtcatta
     8341 tatgcgcaac gggggctgta ttgcttgggt attttggttt aaaatgttga caatctgacg
     8401 ggggttcccg tttttcggat gtgtttgtat tgtttgttca aaggaaattt tatgaaaaaa
     8461 atcgaggcga ttgtcaaacc gttcaagctc gacgacgtgc gcgaggcgtt gacggaaatc
     8521 ggcattacgg gcatgaccgt cagcgaggtc aaagggttcg gcaggcagaa ggggcatacg
     8581 gaaatctatc gcggtgcgga atacgccgtc gatttcctgc ccaaggtcaa aatcgagttg
     8641 gtgttggcgg atgatgccgt ggaacgcgcg attgacgtga ttgtcgaggt ggcgcgttcg
     8701 ggcaaaatcg gcgacggcaa gatttttgtg ctgccggtcg aggaggcaat ccgtatccgc
     8761 acgggcgaac gttcggacgc ggcggtttga gcgtttggaa tggaaatgcc gtctgaaagg
     8821 ctttcagacg gcatttgtt
//
LOCUS       DBJGGI010000074         8281 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.074, whole genome shotgun sequence.
ACCESSION   DBJGGI010000074 DBJGGI010000000
VERSION     DBJGGI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.074"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            186..470
                     /locus_tag="ACSIGJ_001794"
     CDS             186..470
                     /locus_tag="ACSIGJ_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HHQ1330608.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMPVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWRDLWFDII"
     gene            complement(950..3100)
                     /gene="yccS"
                     /locus_tag="ACSIGJ_001795"
     CDS             complement(950..3100)
                     /gene="yccS"
                     /locus_tag="ACSIGJ_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HHQ1330609.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYEALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDGNDSPDIRHLSRLLDNLGS
                     VDQQFRQLRHSDSPAENDRMGDTRIAALETGSFKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVYQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIAGTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSSGTYLQKIAERLKTGETGDDIE
                     YRITRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPDMGPDDFQTALDTLRGELGTLRTRSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
     gene            complement(3217..4443)
                     /locus_tag="ACSIGJ_001796"
                     /pseudo
     CDS             complement(3217..4443)
                     /locus_tag="ACSIGJ_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
     gene            complement(4607..5650)
                     /gene="pilT"
                     /locus_tag="ACSIGJ_001797"
     CDS             complement(4607..5650)
                     /gene="pilT"
                     /locus_tag="ACSIGJ_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HHQ1330610.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALSSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVNRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEA
                     ARRRAQNSESMSF"
     gene            5770..6456
                     /locus_tag="ACSIGJ_001798"
     CDS             5770..6456
                     /locus_tag="ACSIGJ_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HHQ1330611.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGREPHSVSLIAVSKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSRQRRSLMPPLQVCVEVNIAGEAAKHGVAPEEAVALAVEVAKLPNLVVRG
                     LMCVAKANSSETELKTQFQTMQKLLADLNAAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            6456..6863
                     /locus_tag="ACSIGJ_001799"
     CDS             6456..6863
                     /locus_tag="ACSIGJ_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HHQ1330612.1"
                     /translation="MEIRVIKYTATAALFAFMVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPLSLEDYEIPLSDGNRSVRANEYESAQKSYFYRKIGKFEACGLDWRTR
                     DGKPLVERFKQEGFDCLEKQGLRRNGLSERVRW"
     gene            6894..7703
                     /gene="proC"
                     /locus_tag="ACSIGJ_001800"
     CDS             6894..7703
                     /gene="proC"
                     /locus_tag="ACSIGJ_001800"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HHQ1330613.1"
                     /translation="MNRVEIMNVYFLGGGNMAAAVAGGLVKQGGCRIHIANRGAEKRE
                     RLGKELGVETSATLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVGTL
                     SRYLGGSRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDE
                     AQMHSITGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGE
                     DFEKLQKNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVCRSQEMERQYQ"
     gene            7852..8268
                     /gene="dksA"
                     /locus_tag="ACSIGJ_001801"
     CDS             7852..8268
                     /gene="dksA"
                     /locus_tag="ACSIGJ_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HHQ1330614.1"
                     /translation="MVKLTEQDILNWIGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     VTTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVEAQERRERMKKQFAD"
BASE COUNT         1733 a         1799 c         2722 g         2027 t
ORIGIN      
        1 ggaccttaga acaacagcaa tattcaaagg ttagctgaag ctttagagat tctggattcc
       61 cgctttcgcg ggaatgacga agagttgcgg gaatgatgga aagctatggg gataacgaag
      121 ggttaaagta atcactggat ggtgttcgcg ggaatataaa tttaaataat tcaaaagggt
      181 attatatgca gcctgcggtt tatattttag caagccaacg taatggcacg ttatacattg
      241 gcgttacatc tgatttggtg caacgtattt accaacatag ggagcatttg attgaaggat
      301 ttacatcacg gtacaacgtt actatgccgg tttggtatga actgcatcct acgatggaga
      361 gcgcaattac tcgggaaaaa cagttgaaga aatggaacag ggcttggaaa ttgcaactga
      421 ttgaagaaaa taatgtttct tggcgggatt tatggtttga tattatttag cccgtcattc
      481 ccgctcaggc gggaatccgg cttgttcggt ttcggttttt tttgaggttt cgggcaactt
      541 ctaaaccgtc attcccgcac aggcgggaat ctagaacgta aaatctaaag aaaccgtttt
      601 acccgataag tttctgtgcc gacagaccta gattcccact ttcgtgggaa tgacgggatt
      661 tgagattgcg gcatttatcg gaaaaaacag aaaccgctcc gccgtcattc ccgcgcaggc
      721 gggaatctag acattcaatg ctaaggcaat ttatcgggaa tgactgaaac tcaaaaaacc
      781 ggattcccac tttcgtggga atgacgggat tttaggtttc tgtttttggt tttctgtttt
      841 tgagggaatg acggttcagt tgctacgcat ttaccctgcg caaagcctta tccactatct
      901 tgcaacctgc ctgacaatcc gccccccttt acaaaatgcc gaaatttttt caggctgcgt
      961 tttggggctg cctgtgcgga atttgtcggt aggcgcggta gtagggttcg agctgccggg
     1021 cgatgagttg gagctgttgg aggaggatgt ggctttgtgt tccgctgctg cgggtgcgga
     1081 gggtgccgag ttcgccgcgc agtgtatcca atgccgtctg aaagtcgtcg ggtcccatgt
     1141 cgggcaggtg ttggaagatg tgggcggtgt gttcggcggc aaggtggaac tgtgcggtaa
     1201 agtcggggct gcattcttcg tgcatttcgc tgcggtatgc gccgagggcg gagatgtagc
     1261 cggtcagggc gtagccggtt ttgagcaggg taaagccggg ttgcaggctg tcggcgaatt
     1321 ttgcgggttc gctgctcatg tcggaaaggg tgctgctgag ggcggcggtg tgttcgtggg
     1381 cgcggcggcg ggtgatgcgg tattctatgt cgtcgccggt ttcgccggtt ttgaggcgtt
     1441 cggcaatttt ttggaggtat gtgccgctgc tgcatacggc aagggcggcg gtgcgttcga
     1501 gcgtgaggta tttccagtct ggccacaggt agctgaccgc cgcccaggca agggatgcgc
     1561 cgataatggt gtcgatgatg cgcacgggca tggcggcgta tacgtccaaa cctgcgaggg
     1621 agaggctggt cagtgcctga atggtgatga agaaggtgga aaaactgtat ttgtaggtgc
     1681 gggtcatgaa aaagagggtg gtaccggcga tgacgatcca gagtttggtt tcgacggagg
     1741 gggtgaagta ggggacgagc gagccgacga ttacgccgag tacggtgccg gcgatgcgtt
     1801 ggtacacgcg gcttttggtg gcggtgtagt tgggttggca gacgaaaagg gcggtcagca
     1861 gtatccagta gccgaggttg aggttgaggg cttcgacgat ggtgcaggcg gcggcaacga
     1921 cgagggacag gcggacggca tggcggaata cgcctgattc gaggttcagc tgcggacgga
     1981 ttgcctgcca ggtgtttttg aagctgccgg tttcgagggc ggcgatgcgg gtgtcgccca
     2041 tgcggtcgtt ttcggcgggg gagtcgctgt gtcggagttg gcggaactgc tggtcgacgc
     2101 tgccgaggtt gtcgagaagg cggctcaggt ggcggatgtc gggactgtcg ttgccgtctg
     2161 aaaggaggcg cagcgattgg cggcagcctt cgatggcgcg tccgaggcgt ttgctgtaaa
     2221 cgtagtcttt gcttgcgcgc agggcttgtg cggtgttgcg gcaggcttgt ccctgcattt
     2281 cgagcaggcg gtggatgcgg aagatgatgt cggtgttttt gaatttttcg gacatctctt
     2341 ggtagtcgac gtgggcggag ctgatgcgtt cgtggatgtc ttgggcggcg aagtagtagc
     2401 gcagcatttt ggcggtgcgc gggtggcggt gtttgccgcg caaacggtaa aacagggcgg
     2461 aacggcattg gttgaaggcg gtgatgacgc cggtgttgct catggcgagg tcgatgtggc
     2521 ggttgcctat ccaggctgcc tcatcggggt cgaagaagtc ggctttggct tcgaggtagc
     2581 cgccgagtgc ttcgtaggcg ttggcgacgc tttcttggac ggggcggtgg ggcaggatga
     2641 tttggaacag gatgatggcg gtgctgtaca gtacggtgcc gcataaaatc atgaaggggt
     2701 tggtcagcca gtaggtttcg ggggtgtagg taagcgtggt gtaggtggcg acggcgagtg
     2761 cgccgaaggc gaaggtgcgg tatttcagcc cgaccgcgcc taaaatggta aagccgaagg
     2821 tcatcagggt catggcgagg atgaagggca gccctgtgcc gagggtgctt tgcgccgtga
     2881 gcgaggagag ggtaaacagg gcgacggtgg cgatgatgtt tttcagccgt ccggtcaggc
     2941 ggttgtccaa atcgaccagg ccgccggcga tgatgccgag tacgaagggc atggcgagct
     3001 tgggttcgcc tagctgccag acgatggagg cggcggtaaa gacactggcg aaaacgggaa
     3061 gcgaggtaat gagcagaggc ttgaggagtg gggttttcat ggttttaccg gtttattgtt
     3121 atgaagtgaa taaagtgtgg cacatgaatg gggcggataa aatcatgccg tctgaaaacg
     3181 gggatgcggt tttcagacgg cattgggttt tgcggatcag gaaatgaggt tgagaccgtt
     3241 gaccctgtcg taaaggagtt cgggcgtttt gccttctttg tgcagttgga tgtgcaatcg
     3301 caggttgttg gcggaaacgg actggcgcag ggcttcttcg taactgatga tgccgtgacg
     3361 gtacagttcg aaaaggtttt ggtccatcgt ctgcattccg tcggttttgg cggtttccat
     3421 gattttactg atgttcatca ggtcgccctt caggatgaag tcttggatgg cgggcgtgtt
     3481 gatgagcaag tcgacaaccg ccgtcctgcc cgttttgtct tttttgaggg cgaggcgttg
     3541 gcagatgatg ccggtcagat tgagggcgat gtcaatcagt atttggttgt gctgttcttt
     3601 ggggtagaag ttgagtatgc gttcgagcga ctacggcgcg gtgttggcgt ggagcgtaaa
     3661 aatgcacagg tggccggttt gggcgagctg catcgcgtat tccatacttt ccctgctgcg
     3721 gacttcgccg atacagacca cgtctgggga ttggcgcata gcgttttgta ccgccgtctg
     3781 ccagtttatg gtgtcgacgc cgatttcgcg ctgggtaaag atgcagcggc gcggtttgta
     3841 gataaactca atcgggtctt cgatggtaac gatatggccg ggcagggttt tgttgcggtg
     3901 ttcgagcata gtcgccatcg tggtggattt gcccgaaccg gtaggcccga cgataatcag
     3961 cagcccgcgc ggtgcgacgg cgaggtcttt gagtttttcg ggcaagccca attcccgcat
     4021 ttgcgggatg acgtggttga tgcgccgcaa aaccaaacct gcgctgcctt ggctgtagta
     4081 ggcgttggcg cggtagcgcg tgccgctgcg cgattggacg gagtagttga tttcgccgtc
     4141 gcgccggaat atttccgatt gttcggcgtt catcgtcgat gcggcgatgg cggcggtttc
     4201 ctcgcccgtc agagcctttt gcggctgcgg ggttaatgcg ctgttgattt tcaacgaggg
     4261 cgggaatcct ttgctgataa ggatgtcgga cgcgttttgt gcttctgcgg tttcgcacag
     4321 gcggtcgagc agcgggtgga agtgtgcgcc gatttcggcc ggggtttcgg attcggcttt
     4381 gtttttttga gaatacactt gaaccgtttc gtccaagatg tcgtgcaggt tatcggtatt
     4441 catcgttagc ttcttttcgg tttaagcctt gcagtttgcg gcggcaggtt tcaacaggaa
     4501 ggcggacgct tcctgttcgg aagggtatgc cgggcgggat gccgcgtccc gcccggcgtg
     4561 tttgcgcctt gttttcccgc cggtatggcc ggaaggcggt tgtgtgtcag aaactcatac
     4621 tttcgctgtt ttgcgcgcgt ctgcgtgcgg cttccggtgc gatcagccct tggcgcacca
     4681 gcgattgcag cgattggtcc atcgtctgca taccgctcgc ctgcccggtt tgcaggacgg
     4741 agttaatctg cgtgattttg ttttcgcgga tgaggttgcg gacggcgggg ttggcaatca
     4801 ggatttcgtg cgaggcgaca cggccgttgc cgtcgtgcgt tttcagcagg ttttgggaga
     4861 tgacggcggt cagcgattcg gacagcatgg aacgcaccat ttctttctct ccggcgggga
     4921 atacgtccac gatacggttg acggtttttg ccgcgccggt cgtgtgcagc gtgccgaaaa
     4981 ccaagtgtcc ggtttcggcg gcggtcagtg ccaagccgat ggtttcgggg tcgcgcatct
     5041 ctccgacaag gataacgtcg gggtcttcgc gcaatgcgga actcagcgcg ttggcgaagc
     5101 tgagggtgtg ctggtgcagc tcgcgttggt taatcaggga ttttttgctt tggtggacga
     5161 attcgatcgg gtcttcgatg gtcaggatgt gtgccggctg ggtttcgttg atgtagttga
     5221 tcatcgcggc aagcgtggtc gatttgcccg aaccggtagg gccggtaacc aataccatgc
     5281 cgcgcggcga ttctgcgatt ttttggaaaa tgctcggggc tttcaattct tccagcgata
     5341 agacggtgct gggaatggtg cggaatacgg cggcggggcc gcggccggtg ttgaaggcgt
     5401 tgacgcggaa tcgggcgacg ttgggcagtt cgaacgagaa gtcgacttcc aagttttgct
     5461 ggtagatttt ccgctggtgg tcgttcatca ccgaagttac catattgccg acctcttccg
     5521 cgctcatttc gggaaggttg atgcgccgca tgtcgccgtg aacccgaatc ataggggata
     5581 tgcccgaact caggtgaagg tcggatgctt tgtttttagc gccgaaggcg agtaagtcgg
     5641 taatctgcat aatgcggctc tgtttagtat aatgtttcga ttggttggaa tgattctaac
     5701 aaccttgatt ataccgccct gactggaggg gtttcaactg tttaatcatt tttaattagg
     5761 ggatgatcta tgacggtgtt gcaagaacgt tatcgtgagg tgtccgaccg tatcggaaaa
     5821 ttggttctgc aggcgggcag ggagccgcat tccgtcagcc tgattgccgt cagcaagacc
     5881 ttcccttcag acggcatccg cgaagtttac gctgccggac agcgtgattt cggcgagaac
     5941 tatattcagg agtggtacgg caaaacggag gaattggcgg atttgaccga catcgtgtgg
     6001 cacgtcatcg gagatgtgca gtccaacaaa accaaatttg tcgccgaacg cgcgcattgg
     6061 gtgcataccg tatgccgtct gaagaccgcc gtccggctga gcaggcagcg tcgctcctta
     6121 atgccgccct tgcaggtgtg tgtcgaggta aacattgcgg gcgaggcggc aaaacacggt
     6181 gtcgcgcccg aagaagcggt cgcgcttgcc gtggaagtgg cgaagctgcc caaccttgtc
     6241 gtgcgcggac tgatgtgcgt tgccaaagcc aacagcagtg aaacggagtt gaaaactcag
     6301 tttcagacca tgcaaaagct gcttgccgac ctcaatgcgg ctggcgttaa ggcggacgtg
     6361 ctgtctatgg ggatgtcgga cgatatgcct gccgccattg agtgcggtgc gacacacgtc
     6421 cgtatcggca gcgcgatttt cgggaaaagg ggctgatgga aattcgggta ataaaatata
     6481 cggcaacggc tgcgttgttt gcatttatgg ttgcaggctg ccggctggcg gggtggtatg
     6541 agtgttcgtc cttgtccggc tggtgtaagc cgagaaaacc tgccgccatc gatttttggg
     6601 atattggcgg cgagagtccg ctgtctttag aggactacga gataccgctt tcagacggca
     6661 atcgttccgt cagggcaaac gaatatgaat ccgcgcaaaa atcttacttt tataggaaaa
     6721 tagggaagtt tgaagcctgc gggttggatt ggcgtacgcg tgacggcaaa cctttggttg
     6781 agaggttcaa acaggaaggt ttcgactgtt tggaaaagca ggggttgcgg cgcaacggcc
     6841 tgtccgagcg cgtccgatgg taaaaaattg ggaatgaatt tagtaaggta attttgaata
     6901 gggtagaaat aatgaatgtt tattttctcg gcggcggaaa tatggcggct gccgttgcgg
     6961 gcggattggt caaacaaggg ggatgccgca tccatatagc caaccgtggt gcggaaaagc
     7021 gcgaacgttt gggaaaagag ttgggggtcg aaacttcggc aaccctgccg gagcttcatt
     7081 ccgacgatgt tttaatcctt gccgtcaaac cgcaggatat ggaagccgcg tgcaaaaata
     7141 tccgcaccaa cggtgcattg gtgctttctg tcgcagccgg attgtcggtc ggtacgctca
     7201 gccgttacct cgggggaagc cgccgcattg tccgggttat gccgaataca cccggaaaaa
     7261 tcgggctggg cgtatccggt atgtatgccg aagcggaagt atcggaaaca gaccgcagga
     7321 ttgccgatcg aatcatgaaa tcagtcggtt tgaccgtttg gttggaagat gaagcgcaaa
     7381 tgcacagcat taccggcatc agcggcagcg ggccggctta tgtgttttat ctgctggacg
     7441 cattgcaaaa tgccgccatc cgacaagggt ttgatatggc agaagcacgc gcgctcagtc
     7501 tggcaacgtt taaaggagcg gttgcccttg ccgagcagac gggtgaagat ttcgagaagc
     7561 ttcaaaaaaa tgtaacgtca aaaggcggga caacccacga agccgtggaa gccttcagac
     7621 ggcatcgtgt cgccgaagcc ataagtgagg gcgtttgtgc ctgtgtgtgc cgttcgcagg
     7681 aaatggaacg gcaatatcaa taatgtaaag aaaataaaaa aaaccaatcc aaaacgtgtt
     7741 atgatgcgcg ttttcaaaaa cgccttaggc aataagcctt ataaaaatca aaggaataaa
     7801 gccactttgt agtgctttgt ttttttgcgg tgaaccgaga gggcatacat tatggtaaag
     7861 ctgacagaac aagatatttt gaattggatt gggccggaag acgattatat gaatgacgac
     7921 catttggctt ttttccgcga attgctggta aaaatgcaag acgaactcat cgaaaacgcc
     7981 tccgttacaa cagggcatct ccaagaacac gaatcagccc ccgatcctgc cgaccgtgcc
     8041 acacaggaag aagagtacgc attggaactc cgtacccggg atcgggaacg aaaacttctc
     8101 agtaaaatac aggcgaccat ccgcaatatt gatgaagggg actatggatt ctgtgccgat
     8161 acgggagaac ctatcggtct gaagcggctg ctggcacgcc cgacagccac tttatccgtt
     8221 gaggcccaag aacgccgaga gaggatgaag aaacagtttg ccgactgatg gaggcaaaca
     8281 a
//
LOCUS       DBJGGI010000075         8122 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.075, whole genome shotgun sequence.
ACCESSION   DBJGGI010000075 DBJGGI010000000
VERSION     DBJGGI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8122
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.075"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            33..286
                     /locus_tag="ACSIGJ_001802"
                     /pseudo
     CDS             33..286
                     /locus_tag="ACSIGJ_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173384.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
     gene            355..1032
                     /locus_tag="ACSIGJ_001803"
     CDS             355..1032
                     /locus_tag="ACSIGJ_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HHQ1330615.1"
                     /translation="MTFFKPFTVVLTASALALSGCVADPVTGQQSPNKSAMYGLGGAA
                     VCGIVGALTHSGKGARNSALACGAIGAGVGGYMDYQEQRLRQNLAGTQIEIQRQGNQI
                     RLVMPESVTFATGSAALGGSAQYALNTAAQTLVQYPDTTLTINGHTDNTGSDAVNNPL
                     SQHRAQAVAYYLQTRGVAASRLTVYGYGSHMPVASNATVEGRAQNRRVEILINPDQRA
                     VNAARHM"
     gene            complement(1306..2076)
                     /gene="xth"
                     /locus_tag="ACSIGJ_001804"
     CDS             complement(1306..2076)
                     /gene="xth"
                     /locus_tag="ACSIGJ_001804"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HHQ1330616.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRSVPQDVHFGLPSLPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRHGKLVLLGDFNIAPADADCYDPE
                     KWYEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVTSEMAAVLKDVRIDLETRALERPSDHAPVAAEFDL"
     gene            complement(2136..2417)
                     /locus_tag="ACSIGJ_001805"
     CDS             complement(2136..2417)
                     /locus_tag="ACSIGJ_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HHQ1330617.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIVELAKSLSLSAT
                     AVSCHLNRLRAGGLVDFTRYHRIIEYRLVSEDAATILRTIRDLENKRAA"
     gene            2670..2921
                     /locus_tag="ACSIGJ_001806"
     CDS             2670..2921
                     /locus_tag="ACSIGJ_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330618.1"
                     /translation="MDIFRESRPFCATGCPTPTSGSAFHHRRRRYGQDRIGRAFLTMH
                     RILDKENQIHTDMALALPAVVKDAVAIWADMDALDRCSG"
     gene            complement(3152..4033)
                     /gene="nadC"
                     /locus_tag="ACSIGJ_001807"
     CDS             complement(3152..4033)
                     /gene="nadC"
                     /locus_tag="ACSIGJ_001807"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HHQ1330619.1"
                     /translation="MPSENPLFALPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPCVRFQAEIQDGQAVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     ANHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGPLTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
     gene            complement(4109..5047)
                     /locus_tag="ACSIGJ_001808"
     CDS             complement(4109..5047)
                     /locus_tag="ACSIGJ_001808"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HHQ1330620.1"
                     /translation="MDAYPEAEAPLQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQKRLKHIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIAEAAEPQANFDFALTGQTMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPGFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLYRFRDNVLSDRLISDIGLPLGSR"
     gene            5249..6361
                     /gene="nadA"
                     /locus_tag="ACSIGJ_001809"
     CDS             5249..6361
                     /gene="nadA"
                     /locus_tag="ACSIGJ_001809"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HHQ1330621.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDHETAGRL
                     KDEIKALLKRKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVAVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKHALTGGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            6413..7921
                     /gene="nadB"
                     /locus_tag="ACSIGJ_001810"
     CDS             6413..7921
                     /gene="nadB"
                     /locus_tag="ACSIGJ_001810"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HHQ1330622.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIGKHVADTLEAGAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDR
                     NHNDLHLTREGGHTCRRIAHVADYTGEAVMQSLIIQIRRRPNIRVYERQMALDVQTES
                     GAACGLTVLDCRTQETYRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     IENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQSGERFMPHYDRRAELAPR
                     DIVARAIAAETAKQTQDFVSLDISRQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPSGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQG
                     IADGQAFQIEPFQRPSESPSAETDIFSNDLQNTFSRPVLQTFNQRHLGILRNDTDLHR
                     AIDQLRLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
BASE COUNT         1771 a         2388 c         2269 g         1694 t
ORIGIN      
        1 tgatttttgt taatccacta tattcctaaa cccaccatcc gcgcctgccg cacgtacacg
       61 tcgacaaacc gaaaatatgg ctgctcggcg gcatcgtcgg cgcgtggggc tgccataaat
      121 cggacaccat ttctccctgc ccgtttcggc attattgccg ataccgggtg caggctcggt
      181 cggctacgat gtgaaggtac gtttttttgc ggatgtggct gcggtttcgg cgcaaatcac
      241 gataaacccg cgttgtttcg ggaaaaatcg ggtaatttca ggttaattgc actttttcag
      301 acggcatgat tgcttatagt tgtacggttt gacatatgca cggaaggaaa cgccatgact
      361 ttcttcaaac cctttaccgt cgtgctgacc gcatccgcac tcgcgctttc cggctgcgtt
      421 gccgaccccg taaccggaca gcagtcccca aacaaatccg ccatgtacgg tttgggtggc
      481 gcggcagtgt gcggcatcgt cggcgcactg acccacagcg gcaaaggcgc acgcaattcc
      541 gcgcttgcct gcggcgcaat cggcgcaggc gtgggcggct atatggacta ccaagagcag
      601 cgtttgcgcc aaaaccttgc cggcacgcaa atcgaaatcc aacgccaagg caaccaaatc
      661 aggctggtga tgcccgaaag cgttaccttc gccaccggca gcgcggcgtt gggcggcagt
      721 gcgcaatacg ccctgaacac tgccgcacag acgctggtgc agtatcccga cacgacgctg
      781 accatcaacg ggcacaccga caacacaggt tccgatgcag tcaacaatcc gctttcgcaa
      841 caccgcgccc aagcggttgc ctactatctg cagacgcgcg gcgtggcggc ttcgcgcctg
      901 acggtttacg gctacggttc gcatatgccg gtcgcgtcca acgctacggt tgaaggccgc
      961 gcgcaaaacc gccgcgtcga aatcctcatc aaccccgacc aacgcgccgt caacgccgca
     1021 cggcacatgt aacgcgccct gcaacgccaa tgccgtctga atgtttttca gacggcattt
     1081 ccgtattcgc aagcacagcc gtaccccgac tgtaatgcta tggataacgt ctcgccgttt
     1141 gccgggaaac cgcagattgc agccggtggc gtgcaagtgg gcccaacctt gcagacgtta
     1201 ccgccatacc gcgatacccc tgtaacctca tcgtttaaac agatattcaa tcaaaatgcc
     1261 gtctgaaacc ctgcgaagtt tcagacggca tttgcctttc ccgaattaca aatcaaattc
     1321 tgccgccacg ggcgcgtggt cgctcggacg ctccagcgcg cgcgtctcca aatcgatgcg
     1381 gacatccttc aaaacggccg ccatttcgga tgtaaccaat atatggtcga tacgcaggcc
     1441 cagtttgcgt tggaacatcg cgccgcgata gtcgaaccag gtatagaacg cgccttcggg
     1501 gtggacttgg cgcaggctgt cggtcaatcc caaatccagc agattttgaa accactgccg
     1561 ttcgacggac gaacagtgga ttttttcata ccatttttca gggtcgtaac agtccgcatc
     1621 ggcaggcgca atattgaaat cgcccagcag taccagtttg ccgtggcggg tcatttcgtc
     1681 gcggacaaac tccgtcagtg cggcaaacca ctgttcctta tatttgaatt tggggctgtc
     1741 caaagcctcg ccgttgacgc aatagacatt gatgacgcgc acgccgccta cggtcgccgc
     1801 aatcacgcgg cgttgcgggt catccggcag ggaaggcaaa ccgaaatgca cgtcctgcgg
     1861 cacgctgcgg ctgacgattg ccacaccgtt gtaggttttc tgcccgctcc aaacacagtg
     1921 ccagcccatc atctgcaagg cggcggccgg aaatttgtcc tgatcgagtt tgagttcctg
     1981 caaaaccaaa atatcgggcg gattgtcggc aagcaggttt tgcacctgcg gcagccgcac
     2041 attgagcgaa ttgacgttcc aagtggtgat tttcataaca tttccgaaaa atgccgtctg
     2101 aaacgttcag acggcaaaag aaaaggattc taacactatg ccgcgcgttt gttttccaaa
     2161 tcgcggattg tccgcagaat cgttgccgcg tcttcggaaa ccaggcggta ttcgataatg
     2221 cggtggtaac gcgtaaagtc caccagaccg cccgccctca ggcggttcaa atgacaggaa
     2281 accgcggtag ccgacaggga taaggatttt gccagttcga cgatattgcg ttcgctgtcc
     2341 aacagttgga tcagtatcgc catacgttcg ggatgcgcca taagtttgag tatggtgtgg
     2401 agcggtattg tgttcatcgg tattcctaaa accttttccg cccgccaagc ggattgacgg
     2461 gcggcagata tggatgattt tgattatctt tataaatttt attataaatc aattgtttat
     2521 ttctcattgt acaggattgt atgttccttt tcccactatt tttgcaaaca agcgtaaaga
     2581 caggctttca gacggcataa aactgccgcc cctaccctgc ctcaccctgt tgccgaacgg
     2641 aatcatccgt attttttaag aaaaccatta tggatatttt ccgtgaatca aggccgttct
     2701 gcgcgacagg ctgtccaacg ccaacatccg gttcggcatt tcatcatcgc cgccgacgat
     2761 acgggcaaga ccgcatcggt cgtgccttcc taacgatgca ccgcatcctc gacaaggaaa
     2821 accagataca taccgatatg gctttagcgt tgccggcggt agtgaaagac gcagtggcga
     2881 tatgggcaga tatggatgca ttggataggt gttccggctg accgctccgt tactgccgta
     2941 tctttcggct accgttcatc aaatggttcc aatgccgaaa tgcctaaatc gcgcctgcct
     3001 gaaatgctgt tttttctaaa gcatcacgac cttcacgaaa aatgccgtct gaagaatatt
     3061 tcagacggca ttccctccct gaggcaataa ccggattacg gttaagcggt tccttacctg
     3121 acatatcaga cagccgcccg caccctgaaa ctcacgccac gaaatccaaa cctatgtcca
     3181 acgatcggct gctgtgggtc agatagccga gggcgatgcc gtccacgccg gtttgcgcca
     3241 cgcgcttcag gcggtcgaag ccgatgccgc ccgatgcttc gcaatagacg gtgtgtgggt
     3301 gggcggtttg tgtgtggcag cggtttgccg cttctttcag ggtttcgtcg tccatgttgt
     3361 ccagcaaaat ccgttccgcg cccgctgcga tggcttcgtc cagttgtgcc aacgtgtcca
     3421 cttcgatttc cacgcaggtc aacggtccga ccgcctgttt tgcctgccga acggcttggg
     3481 cgatgctgcc gcaataggcg aggtggttgt ctttgatgag cacggcgtcg tccaaaccca
     3541 tgcggtggtt cgcgccgccg cccgccctga cggcgtattt ttgcaggacg cgcagcaggg
     3601 ggatggtttt gcggctgcac acgatgtctg taccgtattc ggcgacctcg gcaacggcac
     3661 gagcggtggc ggtggcgatg ccgctcaagt gcgtgaggta gttgagcgcg gtgcgttcgg
     3721 cggcgagcag cgcgcgggcg ttgccttcga cggcagccag tgtctgacct gcgcggacgg
     3781 cttgcccgtc ttggatttcg gcttggaagc ggacgcacgg atccatcgtc tgaaaggcga
     3841 ggcgcgccaa gtccataccg gcgataacac cgtcttcgcg gctgacgagg aaaagtttgg
     3901 cggttttgtc gggcgcgatg acggcggcgg acgtaatgtc gccgcgcctg cccaagtctt
     3961 cgctcaaggc ttgttccacc atggggcgca acaaggtgtc gggcagggca aagagggggt
     4021 tttcagacgg catggggttt cctttgttaa aaaagattga tgttgcaccg atggggcaaa
     4081 acttcatttg atgccgtctg aaaacgggct aacggctgcc cagcggcagt ccgatgtccg
     4141 aaatcagcct gtcggacagg acgttgtcgc ggaagcggta aagctgcgcg ggacggcctt
     4201 tgctgcccga tacgccggta tccgacggct cgataaggtt ttgctgctga atctggcggc
     4261 ggaagttttg cttgtgcagc aatctgccgc tgatggcttc gacgctgttt tgcagttgca
     4321 gcagtgtaaa tccgggcggc atcagttcga aaatcacggg gcggtatttg attttggcgc
     4381 gcaggcggga cagggcggtc gccaacacgc ggcggtggtc gtggcgcatg gtctgccccg
     4441 tgagcgcgaa gtcgaagttt gcctgcggct cggcggcctc cgctatcaga ccgctttcat
     4501 acaacatttc atagcgttgc aaaacgtatt cttccgacca gttttccggt tcgacccccc
     4561 aacacaaatg aatatgcttg agccgctttt ggcggacttc ctccgtgtcc gccgagtttg
     4621 cccaaatgcg caggcggctg acgacggcgt cgcgctgccc gccgtcggtg cgcaagtctt
     4681 cccatgggaa atagccgtag cagtcctgcc atttcgcgtc gggatgcagg atgctatcgg
     4741 ctgcctcgcg caccagcccc aaatagctga cgtacagcac gggcatgccg tgttcgttgc
     4801 ggcggtgggt atcgacaaag gtgtaaagct gttccacata gcccataggc tgcgaagtct
     4861 gcttggcgac ccacagcttt acgcccgcct gcagggaatt gcgcaggggg gaaagcgggc
     4921 cgttgggcag gagcgtgcct tgggcgacgg tcaacacccg caggccgccg tcggtaacgg
     4981 caatcaatac gggaaccagc tcgacgatgc tttgcagcgg agcttcggct tcggggtagg
     5041 cgtccatgct cggctttctg cgggtgggaa aggctttatt gtaaaccaaa gcggtttgtc
     5101 ggaacgaagc gggtcggttt tgcggtatcg caaacctgtt ttcagacgac cttgcatatt
     5161 atactcaagt tgagcataat gtaaaaacag tttgaaaaac agacggcaaa ccttcatgcc
     5221 gtctgaaaac cagctacaag gaaacatcat gcaaaccgcc gcccgccgct cgttcgatta
     5281 cgatatgccc ctcatccaaa cgccgacttc cgcctgccaa atccgtcagg cgtgggcgaa
     5341 ggttgccgac acgcccgacc acgagacggc aggtcgtctg aaagacgaaa tcaaggcctt
     5401 gctgaagcgg aaaaacgcgg tcttggtggc gcattattac gtcgatccgc tgattcagga
     5461 tttggctttg gagacgggcg gatgcgtggg cgattcgctg gaaatggcgc gcttcggcgc
     5521 ggaacacgaa gccggtacgc tggtagtggc gggcgtgcgt ttcatgggcg agagcgcgaa
     5581 aatcctctgc cctgaaaaaa cggtgctgat gcctgatttg gaagcggaat gttctttgga
     5641 tttgggttgt cccgaagaag cgttttcagc gttttgcgac caacaccccg accgcacggt
     5701 ggcggtgtac gccaatactt ccgcagctgt gaaagcgcgt gccgactggg tggtaacgtc
     5761 ttcggtggcg ttggaaatcg tgtcgtatct gaaatcgcgc ggcgagaaac tgatttgggg
     5821 gcccgaccgc catctcggcg actacatccg ccgcgaaacg ggcgcggata tgctgttgtg
     5881 gcagggttcg tgcatcgtcc ataacgaatt caaagggcag gaactggcgg cgttgaaggc
     5941 agagcatccc gatgctgtgg tgctggttca tcccgaatcg ccgcaaagcg tcatcgaact
     6001 gggcgacgtg gtcggctcga ccagcaaact actcaaagcc gccgtatcgc gccctgaaaa
     6061 aaaattcatc gtggcaaccg atttgggcat cctgcacgaa atgcaaaagc aggcgcccga
     6121 caaagaattt atcgccgcgc cgacggcggg caacggcgga agctgtaaaa gctgcgcgtt
     6181 ctgcccgtgg atggcgatga attcgctggg cggcatcaaa cacgccctga caggcggacg
     6241 caacgaaatc ctgttggaca gaaagctggg cgaagccgcc aaactgcctt tgcagcgtat
     6301 gctcgacttc gcggcaggac tcaaacgcgg ggatgtgttc aacggcatgg ggcccgcctg
     6361 atttgccgtc catatcgccg tttcagacga cctctcaaac aaggacaaca ccatgcaaac
     6421 cgattgcgac gtattgattg ccggaaacgg gctggcggca ctgacgctcg ccctgtcgct
     6481 gcctgaatcg ttccgcatcg tcattttgtg caaaaaccgg ctggacgaca ccgccagccg
     6541 tcatgcgcaa ggcgggattg cggcggcgtg gtcgggagag gacgacatcg gaaaacacgt
     6601 cgccgatact ttggaagcgg gcgcaggttt gtgcgacgaa gccgccgtcc gcaccatcct
     6661 gtcgcagggc aaaccggcaa tcgaatggct gctggcgcag ggcgtggcgt tcgaccggaa
     6721 tcataacgac ctgcacctga cgcgcgaagg cgggcatacc tgccgccgaa tcgcccacgt
     6781 cgccgactac acgggcgaag ccgtcatgca gagcctgatt atccaaatac gccgccgacc
     6841 gaacatccgc gtttacgagc ggcagatggc gttggacgtt caaaccgaat caggcgcagc
     6901 gtgcggactg accgtcctcg actgccgaac gcaagaaacc taccgcatcc gtgcccgcca
     6961 taccgtactc gcaggcggcg gcctcgggca aatttacgcc gccaccacca cgccgcccga
     7021 atgcacgggc gacgccatcg ccatggcgat acgcgcgggt tgcgcaatcg aaaacctcga
     7081 atttatccaa ttccacccca caggcttggc aaggccgtct gaaaacggcc gcaccttcct
     7141 gatttccgaa gccgtgcgcg gcgaaggcgg catcctgacc aatcaatcgg gcgaacggtt
     7201 tatgccgcat tacgaccgcc gcgccgaact cgcgccgcgc gacatcgttg cccgcgccat
     7261 cgccgccgaa accgccaaac aaacgcaaga cttcgtctcg ctcgacatca gccgccaacc
     7321 cgcagcgttc gtccgtcggc atttcccgtc catccatcgg cactgcctgt cccaatgcgg
     7381 cttggacatc acgcgccaag ccatccccgt ccgccccgtg caacactaca cctgcggcgg
     7441 catccaaacc gaccccagcg gcagaacctc cctgccgcag ctctacgcct taggcgaaac
     7501 cgcctgtacc ggactgcacg gagccaaccg cctcgccagc aactccctgc tcgaatgcgt
     7561 cgttaccgcc aggcttgccg cccaaggcat cgcggacgga caagcgttcc aaatcgaacc
     7621 attccaaagg ccgtctgaaa gcccctccgc cgaaacagac atcttttcaa acgacctcca
     7681 aaacacattc agccgccctg tcctgcaaac gttcaaccaa cgccatctcg gcatcctccg
     7741 caacgatacc gacctgcacc gcgccatcga ccaactgcgg ctttggaagc aaaaccaagc
     7801 cgaaccgcac accgcgtccg aatacgaaaa ccgcaaccta ctcgaatgca gcctcgccgt
     7861 cgcccaagcc gcatacaggc ggagacagaa catcggtgcg cattttaata gtgattgtta
     7921 aggttggctg gttagaaaaa aatcgttcaa ataaatttga atagcatggt taagagattg
     7981 gcattcagtt tttatggttg cgtaagttgg gttgaggcct ttgcaaaaaa gcccttcctt
     8041 cggcatccga aacccaaaca caggttttcg gctgttttcg ctccaaatac ctcctaattt
     8101 tacccgaata tccccttaat cc
//
LOCUS       DBJGGI010000076         8102 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.076, whole genome shotgun sequence.
ACCESSION   DBJGGI010000076 DBJGGI010000000
VERSION     DBJGGI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8102
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.076"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(102..2954)
                     /gene="gcvP"
                     /locus_tag="ACSIGJ_001811"
     CDS             complement(102..2954)
                     /gene="gcvP"
                     /locus_tag="ACSIGJ_001811"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HHQ1330623.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDDFVGNTLPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVSDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPKGVKRIANRIHTLASVFADALVSDGLKVVHEVFFDTVTVDFGSK
                     EKADQVFAAALESGYNLRRVNNTQVAAAFHETSVYEDLADLYRAFTGKDTFTFADDVK
                     GRLNAELLRQDDILQHPVYNSYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWTEFSDIHPYAPEAQTAGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHTLTDTHSASAGQT
                     SVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRIAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKSIRREVQKVIDGEWPKDDNPLVNAPHTAADITGEWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(2985..3344)
                     /locus_tag="ACSIGJ_001812"
     CDS             complement(2985..3344)
                     /locus_tag="ACSIGJ_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330624.1"
                     /translation="MTDRPNLLRIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNVVFPLPAHPLAWLVPAGFMVLGGGFPLSLPLVALLVPTCCILARCPPLS
                     RLLCHPCPNHPMSKNSA"
     gene            3343..3522
                     /locus_tag="ACSIGJ_001813"
     CDS             3343..3522
                     /locus_tag="ACSIGJ_001813"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HHQ1330625.1"
                     /translation="MPSEPAGRLSQKTPYRCNFMRLYGVGFALQTAYASRSSLVSQSV
                     HDYINNLGADKPLFI"
     gene            complement(3515..4156)
                     /locus_tag="ACSIGJ_001814"
     CDS             complement(3515..4156)
                     /locus_tag="ACSIGJ_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HHQ1330626.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPEEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGNNNLKSDQIHANGKGYRKFAENLNQFLRKHGFR"
     gene            complement(4146..4697)
                     /locus_tag="ACSIGJ_001815"
     CDS             complement(4146..4697)
                     /locus_tag="ACSIGJ_001815"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HHQ1330627.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIKDDNGLFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFGEADIERLLTDAGIVRNRLKIDAAIF
                     NARQIQALQQEHGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRKTLKYHPKWLDAV"
     gene            complement(4701..5219)
                     /locus_tag="ACSIGJ_001816"
     CDS             complement(4701..5219)
                     /locus_tag="ACSIGJ_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HHQ1330628.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRINTAQNHVWADTLDDMLEAALSNAFNRLDSTRTFVPAS
                     RSGSTDKWTVYIDAFQGSYTGKTLISGYAVLPDGKNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5219..6880)
                     /gene="pqiB"
                     /locus_tag="ACSIGJ_001817"
     CDS             complement(5219..6880)
                     /gene="pqiB"
                     /locus_tag="ACSIGJ_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HHQ1330629.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSGEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQIESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEIANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGMVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGGKMI
                     ELNDQPSASPKLRPHTVYAGDTVIATRGGGLDDLQAKLADLLDKFNNLPLDKTVAELN
                     GSLAELKSALKSANAALSSIDKLVGNPQTQNIPNELNQTLKELRITLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNNSSKDPIPKGSR"
     gene            complement(6873..>8102)
                     /locus_tag="ACSIGJ_001818"
     CDS             complement(6873..>8102)
                     /locus_tag="ACSIGJ_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HHQ1330630.1"
                     /translation="VDCPECGCRTDVPQLDKGEAAFCPRCGHKLFRVGSHPFSGPPAY
                     AAASLILMAFAYSMTYIEVGIPGAASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPV
                     LFLLLCLYVYAALIRKQAYPALRLATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAKV
                     RFGPAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGNNAVQTASEGKTCCSRCLYFR
                     DSAESPCGVCGAELYRRRPKSLSISSAFLTAAVVLYFPANILPIMISSNPAATEANTI
                     FSGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMSVLIAAARFALPAGAKKLSHLYRI
                     TEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLW
                     DKRASDGIAFNETEKYD"
BASE COUNT         1729 a         2106 c         2363 g         1904 t
ORIGIN      
        1 agttatagtc gagatacgtg tatcggtgca accggtctgc cgttgacgaa aatgccgtct
       61 gaaagtgttt cagacggcat ttgtcttcgg ggtttcaaca gtcagtcttc ataattttcc
      121 atcggcgggc agctgcacac gaggttgcgg tcgccgtaca cgtcgtccac gcggttgacg
      181 aagggccaga acttgtgttc gcgcacgaag ggcagcggga agacggcttc ttcgcgagag
      241 tacggatgcg cccattcgcc ggttatatct gcggcggtgt gcggcgcgtt gaccagtggg
      301 ttgtcgtctt tcggccattc gccgtcgatg actttctgca cttcgcggcg aatggatttc
      361 agggcggcga tgaagcggtc gagttcggct ttgctttcgc tctcggtcgg ttcgatcatc
      421 agcgtgccgg caacagggaa ggaaaccgtc ggcgcgtgga agccgtagtc catcaggcgt
      481 ttggcgatgt cggtttcggt gatgccgctt tcggctttga gcggacgcaa atcgacgatg
      541 cattcgtgcg cgatgcggcc gtttttgcct gtatacagaa tcggatagtc ttcgctcaag
      601 cgtttagcga catagttggc gttgagcaat gcccagcgtg ttgcctgttc catgccttgt
      661 ttgcccatca tggtcaggta catccaagta atcggcagga tggatgcaga accgaaggct
      721 gcggcggcaa cggaggtttg cccggcactt gcgctgtggg tgtcggtcag ggtatgcccc
      781 ggtgcgaacg gggcgaggtg ggctttcaag ccaatcggac ccatgcccgg gccgccgccg
      841 ccgtgaggga tacagaaggt tttgtgcagg ttcatgtgca acacgtccgc gccgacttcg
      901 gcgggctgca tgatgccgat ttgggcattg aggtttgcgc cgtccatgta aacctgtccg
      961 ccgttttcgt gaatgatccg gcagatgtcg cggatgcctt cttcgtacac gccgtgtgtg
     1021 gacggatagg taatcatgat ggcggacaag gcatcgcggt gttgttcggc tttggctttc
     1081 aaatcgtcaa tattgacgtt gccgtgttcg tcggtatcga cgacgacgac tttcaaaccg
     1141 agcatggcgg cggtggcggg gttggtgccg tgggcggatt tgggaatcag gcagatgttg
     1201 cggtgtgctt cgccttgggc ttcctgataa cggcggatgg cgagcatacc gctgtattcg
     1261 ccttgtgcgc cggagttggg ctggaaggaa atcgcgtcaa agccggtgat ggctttcagg
     1321 ctgttttcca tatcagcaag caattcgcgg tagccggcgg tttgcgcttc gggagcgtaa
     1381 gggtggatgt cggagaactc agtccaagta atcggcaaca tttccgcagt cgcattgagc
     1441 ttcatggtgc agctcccgag cgaaatcatg ctgcggttca tagccaagtc gcggtcttcg
     1501 agttttttca ggtagcgcaa catttcgtgt tcggtgtggt aactgttgta cacaggatgt
     1561 tgcagaatgt cgtcttgacg cagtaattcg gcgttcagac ggcctttgac atcatcggca
     1621 aatgtgaacg tatccttgcc ggtaaacgcg cggtacaaat cggccaaatc ttcgtatacc
     1681 gacgtttcat ggaatgcagc cgcaacttga gtattgttga cgcggcgcag gttgtaaccc
     1741 gactccaaag cagcggcaaa cacttggtct gctttctctt tgctgccgaa atcgacggta
     1801 acggtatcga agaagacttc gtgaaccact ttgaggccgt ctgaaaccag cgcgtcggca
     1861 aagacggaag ccagtgtgtg aatgcggttg gcgatgcgtt tcacgccttt ggggccgtga
     1921 taaacggcgt acatgcccgc caagttcgcc agcaatgcct gtgcggtaca aatattggat
     1981 gtcgcttttt cgcggcggat gtgttgttcg cgggtggaca aagccatgcg caaggcaggt
     2041 ttgcccgatg cgtctttgga tacgccgatg atgcggcccg gggcggagcg tttgaactcg
     2101 tctttaaacg cgaaataagc ggcgtgcgga ccaccaaagc ccatcggtac gccgaaacgt
     2161 tgggtgttgc ccaaagcaat atctgcaccc aattcagccg gagacttcag caaaaccaag
     2221 ctcatgatgt cggcggcaac agcaacgatt gtgcctttgg ctttcagacg gccgataacg
     2281 tcctgcaagt cttgcacgtc gccgtcttta ccgacgtatt ggaacagcgc gccgaagtat
     2341 tcgccttcat ccgcttgggc aaaatcgctg accaccagct cgaagccgaa atatttggcg
     2401 cgggttttca tcacgtccaa agtttgcgga tacacgcgcg catccacaaa gaaacgctcg
     2461 gatttcactt tgcccacgcg gtgcgccatc gccatcgctt cggcggcggc ggtcgcttcg
     2521 tccaacaaag acgcgcccgc cacagggaaa ccggtcaaat cgatgcacac ctgctggaag
     2581 ttcaacaacg cttccaaacg accttgggca atctccgcct gatacggcgt gtaggcggtg
     2641 taccagcccg ggttttccaa tacgttgcgc aaaatcacgt tcggcacgcg ggtcgggtaa
     2701 taacccaagc cgatatagga cttgttgatc acgtttttcg acgcgatgcc tttcaatttc
     2761 gccaatgcgt ccgcctcggt cagggcttcg ggcaaatcga gttcggacgg catacggatg
     2821 ctttgcggca aggtgttgcc gacaaagtcg tccatgctct tctcgccgac agcggcaagc
     2881 aacgccgctt cgtcgccgaa actcaaatgg cgcgcggcaa attcgttggg gttgaacagt
     2941 tcggacagtt tcatttctac tccttcgggt ttgtagggct gccatcaggc agaattttta
     3001 gacatcggat gattcgggca agggtggcac aaaaggcggg ataatggcgg acaacgcgcc
     3061 agaatgcagc aggtcgggac aagcagcgcc acaagcggca gcgataaggg aaagccgccg
     3121 cccaaaacca tgaaacctgc aggcaccagc caagccaagg gatgagccgg aagcggaaaa
     3181 acaacattga gcagaaccaa aatcacgaca aacagcggca gccccgccat caggttggca
     3241 cgcctgtatg ttgcgtacgg gtcggatgct tcttcaggtt cgcaatattc catgatttca
     3301 gcctgtccgg gctttatccg cagcaggttc ggacggtcgg tcatgccgtc tgaaccggca
     3361 ggccgtcttt ctcaaaaaac gccataccgc tgcaacttca tgcggctgta tggcgtaggt
     3421 tttgcacttc agacggcata tgcgtcccga agcagtttgg tgtcccagtc ggttcatgat
     3481 tatataaaca acttgggtgc ggataaacct ttgtttatct aaacccatgt tttctcaaaa
     3541 attgattcaa attttcggcg aatttccgat agcctttgcc gttggcgtgg atttggtcgg
     3601 atttcagatt attattgccc aaaatttccg cccacgcgcc gccgaacaac ggaatgccgt
     3661 attcctcgga caaatcctca tacagcggat ggtcgctcaa atgcccgaac aacgcgccca
     3721 gtgtgatgtg cggcacgccg acgaggacgg cgggaatgtt ttccttttgc acggtttcga
     3781 tgattttcgc gatattggcg cgggtctgct cctcgggaac tttgcgcaga aagtcgttgc
     3841 cgcctatgcc gacaatcaca agcttgggtt tgcgtgccaa cagcgcgggc aggcgcgata
     3901 gggcttgcgc ggacgtatcg cccgatacgc cgccgttgac aatattccaa cccgtcagtt
     3961 tttgcagttg cgcggggtag gattcgccgg ggtttgctcc gtagccgaag gtgagcgaat
     4021 cgcccaaggc aagcacggtg cttccttcgg gaattttggc gtgggttcgg gcggattttc
     4081 tgccgcaggc ggtaagaagc aacgcgcctg cgccgaggag gaaggttctt ctgttcattg
     4141 gtttttcaga cggcatcgag ccatttcggg tggtatttca gggttttacg gtaaaccgga
     4201 cagctttcgg cgtgcgcgcc tttgaggtag ccggtactca tcagaaattc gccgacgatt
     4261 tcgccgccga cgaatttgaa atgtttttta aagagtttga cccattcgtc tttgcttcgc
     4321 ggatggtgcg cgtcgagcca gttcttgaac gaaccgtgtt cttgttgcaa cgcttggatt
     4381 tgccgtgcat tgaaaatggc ggcatcgatt ttcagacggt tgcggacaat acccgcatcg
     4441 gtaagcagcc gttcaatatc ggcttcgcca aatgcggcga ccgtatcgat gtcgaaacct
     4501 tcaaatgccg tctgaaacgc ctgccgcttc ttcagcatca gcgtccaact taatcctgcc
     4561 tgattgattt ccaacaccag ccgttcaaac agtccgttgt cgtccttaat cggaaaaccg
     4621 tattgcgtat cgtggtaatg tttgttcggg ttgtcggtgt tttcgggaag tgaggcggca
     4681 aattcgcaat agttcacggt ttactcgacc atctgttgcg ccgcctgttt cagtccctgt
     4741 tcgagtgcgg cggtcatggc ggcgtagccg tcaccctgct gttcggtttc gatatggaag
     4801 ggtctgttct taccgtcggg taggacggcg tagccgctga tgagggtttt gcccgtgtag
     4861 ctgccttgga atgcgtcgat atagaccgtc catttgtcgg tactgccgct gcgtgaggca
     4921 ggaacaaagg tgcgtgtgct gtccaaacgg ttgaatgcat tgctcaacgc cgcttcgagc
     4981 atatcgtcca aggtgtctgc ccaaacatgg ttttgtgcgg tgttgatgcg gtaggggtcg
     5041 gtttgataga ccagtccgcc gcgtttgagc ggttcggcaa gacggacttc gacggcggtt
     5101 tcgccgcctt gcgttgcagg acggatgtag cggctgtcgg gcaacacgaa atattgtgtg
     5161 ctttgcacag taccgcaggc ggcaagcgtc agggcggcgg cgatggggaa aaggcgcatt
     5221 atcggcttcc tttcgggata gggtctttgc tgctgttgtt gaaaatcagt gcgttgggtt
     5281 tttctttcaa agtgttaatg acgggttgaa cgtcttttaa ggttttgtcc aaactttgca
     5341 gcgtattttg tacgtctccg tagataggcg attgaggcga tacgccttgc agggttatgc
     5401 gcaactcttt cagagtttgg ttcagttcgt tcgggatgtt ttgcgtctgc ggattgccga
     5461 ccagtttgtc aatggagctt agggcggcat tggcggattt gagtgcggac ttgagttcgg
     5521 cgagcgagcc gttcaattcg gcaacggttt tatccaatgg cagattgttg aatttgtcca
     5581 gcaaatccgc caatttggcc tgcaagtcgt ccaaaccgcc gccccgtgtg gcgatgacgg
     5641 tatcgcctgc ataaacggta tgcggtcgca gcttgggcga ggcggaaggc tgatcgttca
     5701 actcaatcat tttgccgccg gtcagcaggt tgttgctgga gatggtggcg gtcaggcctt
     5761 tgtttaaggc cgtctggaat tgttgtttcc aatgctcttt gctttgctcg tcggcattga
     5821 tttccaaacg ggaaggctcg atgcggatgc gtacgggaat ccagccgttt tcaaacaggt
     5881 gcaggctgtc attgcggtca aaataaggga catcggaaac catgccgaca ttcagccctt
     5941 tgtattcgac aggcgaaccg acggtcagtc cgcgcacgga ttgtttgaaa aacgcggtgt
     6001 agtacagcga gcggtcgtca ggcaggttgg cgatttcgct gcggctgtcg taaagcgtga
     6061 agctgtcctc gcttttgacg tttttactgt ttttggtttt cggcgaatca aatgaaatcg
     6121 cgcctgacag cagggcaggc agaggggcgg aattgagttt gatgccgctg cctgtggttt
     6181 cgatattgat gccgctttcc agccaaaaac ggctggcgga atgaatcagt ttgtcgttgg
     6241 ggctttggat gaagatggtg taatgcacgc tttggtcgga cgggtcgaaa tgcgcgcttt
     6301 cgatttgccc gaccataaag ttttcataca aaacagggct gttgacgttg aggatgcggt
     6361 cgtttttacc aatcaaattc aagcgcagcc cgctttgccc gatggcggta acgggcggaa
     6421 tgtcctgcac ttggaacacg tcttttgcct cgccgctttt gccgggtgta aaagcgatgt
     6481 acgaacccga aagcagcgta cccaaaccgg ttacgccgct ttggtcgata cgcggcttga
     6541 ccacccagaa ctgggtatcg ctgcggatga ggccggatac gtccgcattg agttgggcag
     6601 taacttccac gcctttttgg tcgtcgcgca gtttgattcg ggtaacgcgt ccgacatcga
     6661 tgctcaatac cttaatgacc gtattgttga cttcgatgcc ttccgcgctg tccatcaaga
     6721 gcgtaaccac aggccccctg ttgcggattt ccttaaccca aagccagccg ccggcaatca
     6781 gcgcgatcag cgggaccagc cagacggcgg agaggaaggt gttgtttttg cggacgcgtg
     6841 cttgagcgtg tccgtttgga ggagggctgt tgtcagtcat atttttccgt ttcgttgaaa
     6901 gcaatgccgt ctgaagcgcg tttgtcccaa agcaggcgcg ggtcgaaata ataggcggac
     6961 agcatcgtca gaatcacgac caggcagaaa tagactgccg cactgcccgg aatgacgcgc
     7021 gcggcataag tgtggaacga acacatcaaa ataataatca caaaaatatc aatcatcgac
     7081 cagcggccga ccgcttcggt gatgcggtag aggtgcgaca atttctttgc gcccgccggc
     7141 aaagcgaacc gtgccgccgc aatcaaaacc gacattgccg caatcttcag caccggcacc
     7201 aaaatactcg cgctgaaaat aaccgccgca atcagcctgt cgccctcgtc ccacatataa
     7261 gcgatgccgc taaagatggt gttggcctcc gtggcggcag gattggacga aatcataatc
     7321 ggcaggatat tggcagggaa atacaaaaca accgccgccg tcagaaacgc cgacgaaata
     7381 ctcagacttt tcggccgtcg gcggtacagt tccgcgccgc acaccccgca gggggattcg
     7441 gcactgtcgc ggaaatacag gcagcggctg caacaggttt tgccttccga tgccgtctga
     7501 accgcattat tccccgtcag ccgcccgatt tggaaataca cccaatgctg gggaaccgat
     7561 accgaagtcc gaatcagcat aaccgacagc gcgaacatca gataaaacgc cggcccgaag
     7621 cgaacctttg ccacagacga gagcttgata tacgccacca gagtggaaac aaaaaacaca
     7681 tccaccatca tcgcctgcct caagcgcacc atcacacgcg ttgccaaacg cagcgcagga
     7741 tacgcctgtt tccgtatcag cgcggcatag acatacaggc acagcagcag aaacagaacc
     7801 ggcgcgccga aagtcagcac aaacatcact tcggccaaaa aaccataatc ctgaaacacc
     7861 atcaggcgca tcatctcggg cagcgaaagg acggatgccg cacccggtat cccgacctcg
     7921 atatacgtca tactgtaagc aaacgccatc aaaatcagcg aagccgccgc ataggcgggc
     7981 ggaccggaaa aagggtggct gcccaccctg aagagtttgt gtccgcaacg gggacagaac
     8041 gccgcctctc ccttgtccaa ctggggcacg tctgtacggc agccgcattc cgggcaatct
     8101 ac
//
LOCUS       DBJGGI010000077         7948 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.077, whole genome shotgun sequence.
ACCESSION   DBJGGI010000077 DBJGGI010000000
VERSION     DBJGGI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7948
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.077"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            22..3450
                     /locus_tag="ACSIGJ_001819"
     CDS             22..3450
                     /locus_tag="ACSIGJ_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HHQ1330631.1"
                     /translation="MMSGFSPKPKTIILSLAGAFGALAFADTPNNTEQQKELNTIVVH
                     GKRSADQKGADDVYYKNVSNAYVGKEYLERYRVQSAGDVLKGLNGVYNMNTRTAGGAI
                     TPNIRGITGKGRIPVTIDGTEQTIDVWMNNYGVGDRNYLDPALFRSIAVEKSPALTRG
                     VKSGVGGAMSIRTIEAGDIVPEGQKFGFQLKTEFANNRTRPANNLNQWLGWEDYRTLP
                     LGATADGAGGGSDPITGQQSPQALVVDNFTPPQHKSGRDNWRFGGDRSYMAAAAFKTE
                     LSDGLAAYSYRNKGNYFAGRKGAEGYLNNPVYDLQKCYDEGGSDFNCKNSATFIPNMA
                     RIYHPGVEVLNSNTETKTLLLKNNWHLPGSHRLGWQYMRTDVRFGEINPFHTTYVMNM
                     EEHNETNRPKELSPQAQSIDSTIRTDTYKLGWAWKPENSRWVDLQANLWRIKTNSTRH
                     QSGGMDLSSARPDPFYDVWYWCTQRGRIPPEHVDNYSSCNDLMNDFGVNGLTKEQVLA
                     MTPNDNGQFRVISGAEQKTRVSRTGFDISNRFRLRDRLSMTLAADYQKEKLAEEVEIV
                     NSQDLFNLAGMVTGLTKLAGPRGGERREWGTNLVFDWQAADRLKISAGIRYHNFRGFD
                     TALAEGRARRDPRYQAGDGTQHYADGAYLPYFELAGDQERRDWNVVAEQLRQAYQSGD
                     AAAIAAAVQAEQAHGARYHLLKYYDGSYRTDGYVYIGNDGRAYFDGRFGGVNEAQNQP
                     LYRVRPVYVPFVNGKLDSGALPQHFHDFVSNYQEKVTNPQGLHGTHYRYWAGVGNPID
                     CIRSDAASCLAHNLSGKVTNFQVGYPEYGDDTGLGLKIIGRHYTEEQYWAQPKPIRAH
                     AWAPTIAVSYDLTDNSRLFVRYAQMSRFPSVYEVGSFYNDVAYAGMPKAPNFRFKPER
                     SRSWEIGYSFNFAPYWSRLRAGDMRLTYYRNRIQNVIETTDYFRTVQYDRKDTAGLEW
                     QSRIDTGRFFAALGATYRLKQQMCDRDTAFDYDPYGAKGVPICIEGGYGSTRGYQALQ
                     PKYSINLDAGVRLLGEKLELGLRGIYHSRVNTKQYDQLLQKDLGIIFDTTGKPHHWRS
                     SLTWDVYGRYRLHKNLNVNLGITNLTNRYYLDPMSNVAAPGPGRTVTFGLTAKF"
     gene            3665..4648
                     /locus_tag="ACSIGJ_001820"
     CDS             3665..4648
                     /locus_tag="ACSIGJ_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slam-dependent surface lipoprotein"
                     /protein_id="HHQ1330632.1"
                     /translation="MSIRNKTLLAVLLCGVFGVAAAAPAGGSSQRKSQDPNSDPTTVR
                     PGTSDKQPFGAGKAGLAFSINNGGSWSKSINLDDGPVKYIRAKYQAPINGNDPYANVD
                     GTRNLQYLRLGDVKPVIGWFYNPTLGQVWYENHHNNTEVYSVRQIANPAIPAAPKFGG
                     LVIAKVPGMGGSNGNVFFGEWAPRAGNPQQISTDLNMADGKRTVWYVGDNPTGNTTGV
                     RTATYNVLGINKHTPGQNDFYTGQVTATFGSGITGTMAGRISRSSDHVDFDHAKIDNR
                     NGTFADPSQGMTGRFYGNQAAAMAGYTTRGTGNVGDDVAFGGSATDIETRK"
     gene            4919..6409
                     /locus_tag="ACSIGJ_001821"
     CDS             4919..6409
                     /locus_tag="ACSIGJ_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HHQ1330633.1"
                     /translation="MNKPLSLALFCLLLPITAHADNPADERRRLLDEGSRQTQQYRES
                     GWLDTEQARGEVEENDGYISIGGEIYQVGDTAEELESAIYHALNARQWHKVRQFAARY
                     AKLPRHKPALIHLADALQKRDEGDFRAAGNSFQTALEAEPDNPRLLLEAGRFYAEDNQ
                     NKESAAAFEKVLKTDIPAETRPIVENYLSELGKRRRWHGQISLGYGYNSNVNQGNGIN
                     QCVWEIAGMCLMERTLPAPTDSTFSSYSATAEKTVPLKGHHGVQVRGVLYGNRYTEKD
                     KDSAAMPDYGYRNGSLYAGYAYADARSSFSLLPYFEYDFRNRHTHYRAWGADADWSRT
                     LSPHWRINSHAGAKKTGYGGQSKTYFADFKQYELGAGAEFSITPKSGLLVNFDAARKA
                     YPEKSSSSKEYTARLGAYRLFSGGTYLNAVLLYRRSLYDAASFVSDNKRRSDKQYIMM
                     AAAGFPQWNIKGVYPELRFRRTIAHSNAVYYRYRQNEWLLGFKYRF"
     gene            complement(6477..7874)
                     /gene="aspA"
                     /locus_tag="ACSIGJ_001822"
     CDS             complement(6477..7874)
                     /gene="aspA"
                     /locus_tag="ACSIGJ_001822"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HHQ1330634.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFAAKAEAFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAESGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHRNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
BASE COUNT         1885 a         2314 c         2150 g         1599 t
ORIGIN      
        1 tatattctta ataaggaata catgatgtcc ggattctccc ccaaacctaa gaccattatc
       61 ctaagccttg caggtgcatt tggcgcattg gcttttgcag acaccccgaa caataccgaa
      121 cagcagaaag aactgaatac catagtcgtc catggcaaac gcagtgccga ccaaaaaggc
      181 gcggacgacg tgtattacaa aaacgtctcc aacgcctacg tcggcaaaga atacctcgaa
      241 cgctaccgcg tccaatccgc cggcgacgta ctcaaaggcc taaacggcgt gtacaacatg
      301 aacacccgca ccgccggcgg cgccatcacg cccaacatac gcggtatcac cggcaagggc
      361 cgcatcccgg ttaccataga cggcaccgaa caaaccatcg acgtatggat gaacaactac
      421 ggcgtgggcg accgcaacta cctcgacccc gccctgttcc gcagcatcgc ggtggaaaaa
      481 agccccgccc tcacgcgcgg cgtgaaatcg ggcgtcggcg gcgcgatgtc catccgcacc
      541 atcgaagccg gcgacatcgt gcccgaaggg cagaaattcg gcttccagct gaaaaccgag
      601 tttgccaaca acagaacccg cccggccaac aacctcaacc aatggctggg ctgggaagac
      661 taccgcaccc tgccgctcgg cgccaccgcc gacggcgcgg gcggcggttc tgacccgatc
      721 accggacagc agagcccgca ggcgttggtg gtagacaact ttaccccgcc gcagcacaaa
      781 agcggccgcg acaactggcg cttcggcggc gaccgcagtt atatggccgc cgccgccttc
      841 aaaaccgaac tttcagacgg ccttgccgcc tacagctacc gcaacaaagg caactacttc
      901 gccggtcgca agggtgcgga aggctacctg aataatccgg tatacgacct gcaaaaatgc
      961 tacgacgaag gcggcagcga tttcaactgc aaaaattccg ccaccttcat ccccaatatg
     1021 gcgcgcatct accaccccgg ggtggaagtg ctcaacagca acaccgaaac caaaaccctg
     1081 ctgctgaaaa acaactggca cctgcccggc agccatcggc tcggctggca atatatgcgc
     1141 accgacgtgc gcttcggaga gatcaacccc ttccacacca cctatgtgat gaatatggaa
     1201 gagcacaacg agaccaaccg ccccaaagaa ctgtcgccac aggcgcagag catcgattcc
     1261 accatccgca ccgacaccta caaactcggc tgggcctgga agccggaaaa cagccgctgg
     1321 gtcgatctgc aggccaacct gtggcgcatc aaaaccaaca gcacccgcca ccagagtggc
     1381 ggcatggatt tgtcttccgc ccgccccgat cccttctacg acgtttggta ttggtgtacc
     1441 caacgcggcc ggataccgcc cgagcacgtc gacaactaca gcagctgcaa cgacctgatg
     1501 aatgatttcg gcgtcaacgg cctgaccaaa gaacaggtgt tggccatgac cccgaacgac
     1561 aacggccaat tccgcgtgat ttccggcgcc gagcagaaaa cccgcgtcag ccgcaccggt
     1621 ttcgatatca gcaaccgctt ccgcctcaga gaccgcctga gcatgacctt ggccgccgat
     1681 tatcagaaag agaaactggc ggaggaagtg gaaatcgtca attcgcaaga cctgttcaac
     1741 ctggccggca tggtaaccgg cctgaccaag ctggccgggc cgcgcggcgg cgagcggcgc
     1801 gaatggggca ccaatttggt gttcgactgg caggccgccg accgcctgaa aatctccgcc
     1861 ggcatccgct accacaactt ccggggcttc gataccgccc tggccgaagg gcgtgcccgc
     1921 cgcgacccgc gctatcaggc aggcgatgga acccagcatt atgccgatgg tgcctatctg
     1981 ccgtatttcg aactggcagg cgatcaggag cggcgtgact ggaatgtggt ggccgaacag
     2041 cttcggcagg cgtatcagtc aggcgatgct gccgccattg ccgcggcagt acaggcagaa
     2101 caggcgcacg gcgcccgtta ccatttgctg aaatactatg acggctcgta tcgtaccgat
     2161 ggttatgtgt atatcggcaa cgatggccgt gcctattttg acggacggtt cggcggggtt
     2221 aacgaggcac agaaccaacc gctctatcgg gtgcgccccg tttatgtgcc gtttgtcaac
     2281 ggcaagctgg acagcggcgc attgccccag cattttcatg atttcgtcag caattatcag
     2341 gaaaaagtga ccaatccgca ggggctgcac gggacgcatt atcgatattg ggcaggcgtg
     2401 gggaatccta tcgactgtat aagaagtgat gcggcctctt gccttgctca taacctatcg
     2461 ggaaaagtaa ccaattttca agtcggctat cccgagtacg gtgatgatac cggtctcggt
     2521 ctcaaaatca tcggtcggca ctataccgag gaacagtatt gggcgcagcc caaaccgatc
     2581 cgtgcccatg cctgggcgcc gaccatcgcc gtcagctacg atctgaccga caacagccgc
     2641 ctgttcgtac gctatgcgca gatgagccgc ttccccagcg tgtatgaggt gggcagtttt
     2701 tataacgacg tggcctatgc cggtatgccg aaggccccaa atttccgctt caagccggaa
     2761 cgcagccgca gctgggaaat cggctatagc ttcaattttg ccccgtattg gagccggctg
     2821 cgtgccggcg atatgcgcct gacctactac cgcaaccgta tccaaaatgt gatcgaaacc
     2881 acagattatt tccgcaccgt gcaatacgac cgcaaggaca ccgccgggct ggaatggcag
     2941 agccgcatcg acaccggccg cttctttgcc gcgctcggtg ccacctaccg cctgaaacag
     3001 cagatgtgtg accgcgatac cgctttcgat tacgaccctt atggtgccaa aggcgtgccg
     3061 atctgcatcg aaggcggcta cggcagtacg cgcggctatc aggccttgca gccgaaatat
     3121 tcaataaacc tggatgccgg ggtgcgcctg ttgggcgaga aactggagct cggcctgcgc
     3181 ggcatctacc acagccgggt caacaccaag caatacgacc aactgctgca aaaggatttg
     3241 ggcatcatct tcgataccac cggcaagccc caccattggc ggtcatcgct gacttgggat
     3301 gtttatggcc gctaccggct gcacaaaaac ctcaacgtca acctcggcat caccaacctg
     3361 accaaccgct actatctcga cccgatgtcc aacgttgccg cccccgggcc gggccggacg
     3421 gttaccttcg ggctgactgc caagttctag ccgcagggcg gagtggggtt ttcaggtagc
     3481 ctgcgggtat ggcgggaagg ctacctgaaa acggattgga tataacaaca aacaattgaa
     3541 cactccggac gcacaaaccc aaccggcccc gcttatccga ggtaatggtt tttaccgttt
     3601 tttccgccgg ttcggcggta ataaaacggt ggcttttgtt taactcttaa ttaaggaaac
     3661 gattatgtct atacgtaata aaaccctgtt ggccgtcttg ctgtgcggcg tttttggtgt
     3721 ggctgcggct gctccggctg gtggttccag ccaaaggaaa agccaagatc caaatagtga
     3781 cccgacgaca gttcgccccg gcacctctga caagcaacct ttcggtgccg gtaaggccgg
     3841 tttggcattc agtatcaata acggtggaag ttggtcgaaa tccatcaatc tggatgatgg
     3901 tccggtcaaa tatatccgtg cgaaatatca ggcaccgatt aacggcaacg atccctatgc
     3961 taatgttgat ggtacgcgca atctgcaata tctgcgctta ggcgatgtga aaccggttat
     4021 cggctggttc tacaacccaa cgttgggtca ggtgtggtat gaaaaccacc acaacaacac
     4081 tgaagtgtac agtgtgcgcc agatagccaa cccggcaatc cccgccgcac ccaagttcgg
     4141 cggcctggta atcgccaaag tgccgggcat gggcggcagc aacggcaacg tcttcttcgg
     4201 cgaatgggcg ccgcgtgccg gtaacccgca gcaaatcagc accgacctga atatggcgga
     4261 tggtaaacgc accgtttggt atgtgggcga taatcccacc ggcaacacca ccggtgtgag
     4321 aaccgccacc tacaatgtgc tgggtatcaa taagcacacc ccggggcaga acgatttcta
     4381 taccggtcag gtaaccgcca ccttcggttc tggtataacc gggacgatgg caggcagaat
     4441 cagccgcagt tccgatcacg tcgattttga tcacgccaaa atcgataaca gaaatggtac
     4501 ctttgccgat cccagtcaag gcatgaccgg ccgcttctac ggcaaccagg cggcagccat
     4561 ggccggctat accacacgtg gtaccggtaa tgtcggcgat gacgtagctt tcggcggtag
     4621 cgccaccgat attgagaccc gcaaataaca gctgccatcc gatttggttt ggcaacggct
     4681 aggctcgatt taacccctga ggagtttcaa gctgccgttt tatgccgact gccaagccat
     4741 ataaatcatc cgtccgatac ggattgctgc tcggtttcag gtagcccgtg ttcaacacag
     4801 gctacctgaa atttgatatt tgtaaataaa catcgcctat cccatacggt ttcggcctat
     4861 cggacatttt gggacagcaa ttaaaacctg cgttacttta tcttttcgga aggcaaccat
     4921 gaacaaacca ctcagcctcg ccctcttctg cctgcttctg cccatcaccg cccacgccga
     4981 caaccccgcc gacgaacgcc gccgcctgct ggacgagggc agccgccaaa cgcagcaata
     5041 ccgcgaaagc ggttggctgg acacggaaca ggcacgcggg gaagtagagg aaaacgacgg
     5101 ctatatttcg ataggcggcg aaatctacca agtcggcgat acggcggaag aactggaaag
     5161 cgcaatttac cacgccctca atgcgcggca gtggcacaag gtgcggcagt tcgccgcccg
     5221 ctacgccaaa ctgccccggc acaagcccgc gctgattcat ttggccgatg cgctgcaaaa
     5281 acgggacgaa ggggatttcc gcgcggcggg gaacagtttt cagacggcct tggaagccga
     5341 acccgacaac ccgcgcctgc tgctcgaagc ggggcggttt tatgccgaag acaaccaaaa
     5401 caaagagtct gccgccgcgt ttgagaaagt tctgaaaacg gacattcccg cagaaacccg
     5461 ccccatcgtg gaaaactacc tgtccgaact gggcaaacgc cgccgctggc acggccaaat
     5521 cagcctcggc tacggctata acagcaatgt aaaccaaggc aacggcatca accaatgcgt
     5581 gtgggaaatt gcgggcatgt gcctgatgga gcgcaccctg cccgcgccaa cggattcgac
     5641 cttctcatcc tatagcgcga ccgccgaaaa aaccgtcccg ctcaaagggc atcacggcgt
     5701 acaggtgcgc ggtgtactgt acggcaaccg ctacacggaa aaagacaaag attcggcggc
     5761 catgccggat tacggctatc gcaacggttc gctgtacgcg ggatacgcct atgccgacgc
     5821 acgctcctcc ttttccctgc tgccttattt cgaatacgac ttccgcaacc gccacaccca
     5881 ctaccgcgca tggggtgcgg atgccgactg gtcgcgcaca ctctccccgc actggcgcat
     5941 taactcacat gccggagcaa aaaagaccgg atacggcgga caaagcaaaa cttattttgc
     6001 cgatttcaaa caatacgaac tcggcgcggg ggcggaattt tccatcacgc cgaaaagcgg
     6061 cctgcttgta aatttcgatg ccgcacgcaa agcctatccc gaaaaatcct cttccagcaa
     6121 agaatatacg gctcggctgg gcgcgtatag gcttttttcg ggcggtactt atttaaacgc
     6181 cgtcctgctc taccgccgca gcctgtatga cgcggcaagt ttcgtcagcg ataacaaacg
     6241 gcggagcgac aagcaataca tcatgatggc tgcggcgggt tttccgcaat ggaatatcaa
     6301 aggcgtatat cccgaactgc gtttcaggcg aacaatcgca cacagcaacg cggtgtatta
     6361 ccgctaccgc caaaacgaat ggctgttggg ttttaaatat cggttttgac cagtcgggtg
     6421 ataaaaaatg ccgtctgaaa gggcgcttcc tttcagacgg catttgtccg taatgtttac
     6481 agatgaggat tcatcaggtt ctcgggggag aggatgcggt tgagttcttc ttcgctcaac
     6541 aacccgcgtt ccagtacgac ttcgcgcacg cctttgccgg tctgggcgca gattttgccg
     6601 accaagtcgc cgttgcggtg gccgatgtac ggattcagat aagtcaccag cccgatggag
     6661 ttgaaaacgt aacgctcgca gatttcgcgg ttgaccgtaa tgcctttgac gcatttgtcg
     6721 gataggttga ctgcggcatt gcccaagagg gaaatggttt caaacataca ttgggcgatg
     6781 accggctcca taacgtttaa ttgcagttgc ccggattcgg cggcgaaggt aatcgtcgta
     6841 tcgttgccga tgactttgaa gcagacttgg ttgacgactt caggaatcac gggattgact
     6901 ttggcaggca tgatggaaga accggcctgc aattcgggca ggttgatttc tttcaaaccg
     6961 gcgcgcggac cggaagagag aaggcgcaaa tcgttgcaga ttttagagag tttgacggcc
     7021 gtgcgtttca atgcgccgtg taccatcaca tatgcaccgc agtcggaggt cgcttcgatc
     7081 aggttttcag tcagtttgca aggcaagccg ctgacttcgg agagtttttt gaccaccagt
     7141 tcggcgtagc ctttgggcgt gttcacgccc gtgccgattg ccgttgcgcc caaattgact
     7201 tccaagagca gttggcgggt gcggtcaagg ttgaggattt cctcttccaa caatacttgg
     7261 aaagattgga attcctggcc tgccgtcatc ggtacggcat cttgaagctg ggtgcgaccc
     7321 attttcaaaa cgtctttaaa cgcttcggct ttggcggcaa aggcgttttt caatacggtc
     7381 agtttgtcga gcaattcgcc gatgctgtaa tacacggcaa ggcggaagcc cgtgggatag
     7441 gcatcgttgg tggattggct ggcgttgacg tgatccatcg gattgacgat gtcgtagcgg
     7501 cctttctcat agcccaaggc ttccaatgca aggttggcga tgacttcgtt ggtgttcata
     7561 ttgaccgaag ttcccgcccc gccctgatac acgtcggacg ggaattggtc gaggcagcgg
     7621 ttgttcagca gaacttcgtc gcaagccttt tcaatggcgg cggcgatttc gggttttact
     7681 gcacccaatt cgccgtttgc ctgcgcggtt gcttttttca ccattaccat actgcggaca
     7741 aactgcggca cgtcggaaat tttttgtgtg gagattttaa agttttcaat ggcgcgcagg
     7801 gtgtggatgc cccaatacac ttcggcggga atctcgcggt cgcccaataa atcgtgttcg
     7861 atacggacag tcatgttttt acctttgtaa gtcggataat taatattgaa aaaatgcgcc
     7921 atcggaaaga tgccgcagca ggacgaac
//
LOCUS       DBJGGI010000078         7817 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.078, whole genome shotgun sequence.
ACCESSION   DBJGGI010000078 DBJGGI010000000
VERSION     DBJGGI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.078"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(69..275)
                     /gene="rpoZ"
                     /locus_tag="ACSIGJ_001823"
     CDS             complement(69..275)
                     /gene="rpoZ"
                     /locus_tag="ACSIGJ_001823"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HHQ1330635.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(333..950)
                     /gene="gmk"
                     /locus_tag="ACSIGJ_001824"
     CDS             complement(333..950)
                     /gene="gmk"
                     /locus_tag="ACSIGJ_001824"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HHQ1330636.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSTEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLKGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLEKAEGDLLHIVNACRLKRSRQLGFIADLLENS"
     gene            1078..1677
                     /locus_tag="ACSIGJ_001825"
     CDS             1078..1677
                     /locus_tag="ACSIGJ_001825"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HHQ1330637.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(1723..2511)
                     /locus_tag="ACSIGJ_001826"
     CDS             complement(1723..2511)
                     /locus_tag="ACSIGJ_001826"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HHQ1330638.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDTGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            2837..4192
                     /gene="yegQ"
                     /locus_tag="ACSIGJ_001827"
     CDS             2837..4192
                     /gene="yegQ"
                     /locus_tag="ACSIGJ_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HHQ1330639.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVADMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELGMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESEAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIHDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLSGHSTAKQSQYVGHVTEIDENGWATIEVKNR
                     FAVGDSLEIIHPSGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMQGKEKALIARVLN
                     P"
     gene            4323..4796
                     /locus_tag="ACSIGJ_001828"
     CDS             4323..4796
                     /locus_tag="ACSIGJ_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003824587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HHQ1330640.1"
                     /translation="MLAACQSGRALKNAHTASAGTPAGESCKVIRGDTAGGGKNIIYR
                     CSNGQAAINAATAAGVLESGIPVSFGGGVPVANGRNLVTRQAANGVGKSDSEACEHAL
                     INAARKFQQTAGKLGGRSVTGFHSYFGKQSLQGGQYDCQAGSFHVRAVMRANVVR"
     gene            complement(5005..7378)
                     /locus_tag="ACSIGJ_001829"
                     /pseudo
     CDS             complement(5005..7378)
                     /locus_tag="ACSIGJ_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
     gene            7591..>7817
                     /locus_tag="ACSIGJ_001830"
     CDS             7591..>7817
                     /locus_tag="ACSIGJ_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HHQ1330641.1"
                     /translation="MKITHCKLKKEVQKEPLRSFVPEVTARSAADILGIHPDSAALFY
                     RKIRTVANHRLALAADEVFEGPAGPGASCFGG"
BASE COUNT         1930 a         2215 c         1843 g         1829 t
ORIGIN      
        1 tgttttttca tttcaaaccg tcggactgca cggcggcaga gtgtgccggc gtgcgtttcc
       61 ggcagaattt atttattgcg cgtcaacagt tccgtaccga tatgcccggc tgcgatttcc
      121 cttaaggcgg taacggtcgg tttgttattg cggacatcgt ccacaagcgg cgtattgcca
      181 ttttcaagct ggcgggcccg acgggccgct accaatgtca ggtcaaaatg gttggaaatt
      241 tttccggtac agtcttcggt ggtaatacgt gccatattat ttgctttctt tcaaaaatat
      301 ttaaattggg aaaccggata ttttcgcggt ttttaggaat tttccaacaa atctgcaata
      361 aatcccagtt gccgcgacct tttcagacgg caggcattca caatatgaag caaatccccc
      421 tccgccttct ccaggtcgtc attgaccacg acaaagtcga acaatacgga ctgttcgatt
      481 tcatgccttg ccttcgacag cctcctttgg ataacttccc gactgtccgt cccgcgccct
      541 ttgaggcgcg cggcaagcac gtcgaaagaa ggcggcagga taaagatacc gacagcttcc
      601 ggcagcgcgt tgcgaacctg cgccgcgccc tgaacgtcga tttccaaaat cacgtcatag
      661 cctgccgccg ccaacgcatt cacgccctcc gtgcttgtgc cgtaatagtt gccgaatacg
      721 tctgcgtatt ccaaaaaagc ctcctgcgcg ataagcgatt caaactcttc tttggaaaca
      781 aagtgataat gtacgccgtt tgcttcgcct tcacgcggcg ggcgcgtcgt atgcgacacg
      841 gaaacgcgca aaccgttatg gtttgccaac agccgcgaca ccagcgtggt tttgcccgtg
      901 ccggaagcgg ccgaaatgat aaagatgttg ccttttcgat aagcggacat attttttacc
      961 tgtatatttt ccaaccgatt gtatcacaac ggacacccta tttcatattt gccgatgccc
     1021 atattttgcc gctattgttt tgattgattt ggcaagcggc aggctgacgg ctacaatatg
     1081 gcgttaaaaa catcaaactt ggaacacgca atgctggttc atcccgaagc tatgagtgtc
     1141 ggcgcgcttg ccgacaaaat ccgcaaaatc gaaaactggc cgcaaaaagg catcttattc
     1201 cacgacatca cgcccgtcct gcaaagtgcg gaatacttcc gccttttggt cgatttgctg
     1261 gtttaccgct atatggatca gaaaatcgac atcgttgccg gcttggacgc gcgcggcttc
     1321 attatcggcg cggcactcgc ctaccagctc aacgtcggct tcgtccccat ccgcaaaaaa
     1381 ggcaagctgc cttttgaaac cgtatcgcaa agctacgcgc tcgaatacgg ggaagctgcg
     1441 gtggaaatcc acaccgatgc cgtcaaaccc ggttcgcgcg tcctgctggt cgatgatttg
     1501 gttgccacgg gcggcacaat gcttgccggg ctggaactga tccgcaaact cggcggggaa
     1561 attgtcgaag ccgccgccat tttggaattt accgaccttc aaggcggcaa gaatatccgc
     1621 gcaagtggcg cgcccttatt taccctgctt caaaacgaag gctgcatgaa aggctgaaaa
     1681 ccgaccctgc cgtctgaaac cggcagggtt gttattatac gttcaaatca cacccaaatc
     1741 ttgcaagccc ctcaacacgc cgtcttcatc aacgccgggg caaacatatt tcgccgcttc
     1801 tttcgctgcc tgttccccgt tacccattgc cacgccgaac ccgacttcag acagcatttc
     1861 cacatcgttc aaaccgtcgc cgaacgccat cacgtctgcc atttccaaac ccaatgcttc
     1921 aaccacgctt ctgatgccgt ctgttttcga cattcccgca ggcagcagat cgaccgcttc
     1981 ctcgtgccag cgcaccgttt tcaagccttc ccgttccaca atatccgacc aaagcggcat
     2041 ttcgttttcc tccgcaaaca ccagcatctg atacaccggt ttgcttgaaa aataatcctt
     2101 atcggcaaaa aaatcgctgg cgatatgctg caaggcgcgg cacacgcatt ccgacagcgc
     2161 ggacacggcg atcccctctc cgccgacaaa cgcataatcc atgcccaagc catccaaatg
     2221 cgcacaaacc ctgcccatca aaccggtatc catcggtact tcgcgcacag ttttaccgtg
     2281 cagcagcgca aactgtccgt ttatcgttac cacggcatcc atacccgttt ccgccatcat
     2341 gtccctgacc ttttcgggaa tcgtcgccaa agaccgcccc gttgccaacg ccgtcaatat
     2401 acctttgccg cgcaaagccg ccaccgccgt tttcacggaa gggcgcaagg tatccgtata
     2461 tttgcggtac agcgtatcgt caatgtcgaa aaacacgatt ttgggattca tcatattctc
     2521 tctcgcactc aaactgccgc attgtatccc aagaaggcaa atacttgata aatccttata
     2581 aatttcccgg caaaattgac cgcgaacacg aaaaggcgga taatccgccc atcttcaaac
     2641 acccttcaga cggcatttgc agcaatgccg tctgaaacat ttttacaaag catacaaatc
     2701 atgcttcaac acacaggacg acacataaag caccgcccta ttatgtcgtc ctgattcgga
     2761 aggggttacg cccctcccaa acaaagtctg atcctgccgc cctaaagggc ggggtttcaa
     2821 ccgaaaagga aacacgatga aagcacccga actcttattg cccgccggcg gattggaaag
     2881 aatgcgcgcc gcctacgatt acggcgcaga tgccgtttac gccggcagcc cgcgttactc
     2941 cctgcgcgcc cgcaacaacg aatttgccaa actcgacgtc ctcgagcaag gcattaaaga
     3001 agcgcacgaa cgcaacaaaa aattcttttt gaccgtcaac accctgccgc acaattccaa
     3061 actcaaaacc ttcgttgccg acatggagcc gctgattgcc atgaaacccg acgcgctgat
     3121 tatggcggat ccgggtttga ttatgaccgt gcgcgaaaaa tggccggaaa tgccgatcca
     3181 tctgtccgta caggcgaaca ccaccaacta ctggggcgtg aaattttggc agaacattgg
     3241 tgtcgaacgc atcatcctgt cacgcgaatt gggcatggaa gaaattgccg aaatccgcca
     3301 agaatgcccc gacatcgaac tcgaagtctt catccacggc gcattgtgca tcgcctactc
     3361 aggccgctgc ctgttgtcgg gctatttcaa ccaccgcgat cccaaccaag gcacctgcac
     3421 caactcctgc cgttgggact acaaagtcca caacgccacg gaaagcgagg caggcgatgc
     3481 ccagcttctg caaggtttca actttgaaaa agcccaagaa gaagccaacc aaaactttga
     3541 aggcatcaac ggtcaaaaac gccatcccta cgccgacaaa gttttcctga ttgaagaatc
     3601 caaccgcccg ggcgagatga tgccgattat ggaagacgaa cacggcacct acatcatgaa
     3661 ctccaaagat cttcgcggta tcgaagtcgt cgaaaaactc gccaaaatcg gtgtggacag
     3721 cctcaaagtc gaaggccgta ccaagtctct ctactacgtc gcccgcgtcg cccagtccta
     3781 ccgcaaagcg attcacgatg ccgtcgcagg ccgtccgttt gattacagcc tgttgagcga
     3841 actcgaaggc cttgccaacc gaggctacac cagcggcttc ctcgaacgcc accaaactca
     3901 ggattatcaa aactacctca gcggccattc caccgccaaa caaagccaat acgtcggaca
     3961 cgttaccgaa atcgacgaaa atggctgggc gaccatcgaa gttaaaaacc gcttcgccgt
     4021 cggcgattcg ctcgaaatca tccacccgag cggcaaccaa accatcaaat tggaacaaat
     4081 gacccgcaaa ggccaacctg tcgatgttgc cccgggcaac ggcattcagg tcaaaatccc
     4141 caatatgcag ggcaaagaaa aagccctcat cgcacgcgtg ttgaacccct gagccacaat
     4201 gccgtccgaa ccccgttttc agacggcatt ttgttgaatc ttactgtaca atggcaatga
     4261 tttcccacag caaacaagga catccgacat aaaaaatcct catactctcc gccgccgccc
     4321 tgatgctggc agcctgccaa tccggccgcg cgcttaaaaa cgcgcacacg gcatccgccg
     4381 ggacaccggc cggagaatca tgcaaagtta tccgcggaga caccgcagga ggcggcaaaa
     4441 acatcattta ccgttgtagc aacggtcagg cggcaataaa tgcagccacc gccgccggcg
     4501 tattggaatc agggattccc gtcagcttcg gcggcggcgt accggttgca aacggcagga
     4561 atctagttac acggcaagcg gcaaacggcg ttggcaaatc cgactccgag gcctgcgaac
     4621 acgccctaat caatgccgcc cgcaaattcc agcagaccgc cggcaaactc ggcgggcgca
     4681 gtgttaccgg cttccacagc tactttggca agcaatcgct gcaaggcgga caatacgact
     4741 gtcaggcagg ttcgttccac gtccgcgcgg tcatgcgcgc caatgtcgtc cgttaacata
     4801 tcggcaatca aaaaatgccg tctgaaacat ttttcagacg gcatttttaa tcctgcaaca
     4861 ttactccctg ccctgagttc ggatactgta tcaatataaa accccatcac acagatttac
     4921 ggtaaaaagc cgtccgaatg agttcctgaa aacaattcgg acagctttaa tccccaacga
     4981 ggcaattaat ccaataatac tagattaaaa cttccattcc agcgatacgg cgtaattacg
     5041 gcctgaggcg cggtagcggt ctaagccttt gccatctcgg tcgaccgcgt tggtggtgct
     5101 gtagctatac aaaccgcgca gggaatccca agtggtgtat ttgcggttga acacattata
     5161 tacgcctgca cgcaaagtca ggtttttagc cggtttgtag aagccgtaca tatcaaacac
     5221 ataagccgac ttgttcagcc acgggtaatc ttttaccttt ttctgcaaag gcgtaccccg
     5281 gcccttgttt tcataaacgg tgtattgcgc gtctttggcc tttttcgcac ccagataagt
     5341 caggcgggag aacacacccc atttttcgct cgggcttcgt agtcgacacc ggcaatcact
     5401 ttcggcggct gtgtggacag caggctgttg tcgcccgaca gtttgctttt cgcataaccc
     5461 agcgagccga acaatttcca gccctcagga acaaaagacg ctattttgtc cacattcaga
     5521 cggcctgtca gctcaagacc acggattcgg gccttatcga tattttgcat ctgccattcc
     5581 ggtttttcgg aataaggatt gctacacata ccgtagtagt aattcatctg agtacagccg
     5641 acatcgccgc tggtggtcag cttctgctct tcagacaaga agttgcggta attgttttga
     5701 tacaggttgg catccaaagt acctttttcg ctgcggcctt gcagagacag ggtgtgggtg
     5761 gtgctgcgct cggctttcag gttgggattg ggcagccaat tacccgaacc gtggttgtaa
     5821 gtgaaataca cttcggacgc attggggaca cggtagccgg aagtaatgtc gtaaccgaca
     5881 tgccaagcct gattcagttg cgccgccaaa ccgacaaatc cgctccagcc tttataagta
     5941 ttggctgcag gcggtgtttt gtcacaagca tgacactcgg cattcaattc ctgaggcgtc
     6001 attttggtat gatcgtaacg gatatctgca cggctgctga acacgtcgtt ccattggatt
     6061 tgatcagaca gtgagaaacc ataattagtg gttttcacag ggtgttgaat cgagctggta
     6121 gtacgggata tctgtccgct gaagtaataa tcgtcgcggt ttaagttttc aaactcacgc
     6181 cgactggcga aagttttaaa cgacaagcga tgttggccgc ccagttgcaa cggttggctg
     6241 tccatacgca aagtaaaacg tttgaatcgg gtgtccatgc tgcggttgta tatttcgtcc
     6301 aaatccttct gatttatagt tgcgcgtcca ggtggaataa tccatcggga acgagccttt
     6361 gttgttaacc gccgccactt tggttttctg ataatcgaag tccgccttca aagacgacaa
     6421 ccaatttgaa tcaggcgtcc attcgtaaaa gaggttggca ttgcgccgtc tgtttacgtc
     6481 atcggcttcg cgccaggaag aagcggtcag gttataagac tcttcaaatc gtgtaattat
     6541 gcccctgctg gccgttaaac gatgcgccga tgcggtgctt gtcgttgatt tgataagcaa
     6601 tcttacccaa gaagttgtgg tatttgtgtt tggacggatc agggataccg cgtgccgaac
     6661 cacggatatt cgctccgctg ccagcaccct ctaccggata gccacgctcg cccgcgcttt
     6721 cggtctcatg accgcgacgt tgcgaataca gcaaagcggc atccacgcgg tcgttgctca
     6781 caccgaaacc gagtgtattt gtccattcgc ggttgcggct gctgtaaccg tttttcatca
     6841 tcacgccgaa ttgcctgtcg tccaacagca aatcatggcc ttgcagggtt tggtaattca
     6901 cgccgccacc caatgcgccg ctaccggtat tgaaagagtc agcgcccttc gcgatttcga
     6961 tgttgcgcac gagttcgggg tcgatagaca ggcgcgagct gttgaagttg ccataacgtg
     7021 catacagtga gttttcttcc gaatcaggca ggctcacgcc gtcaatgctg acaccgacac
     7081 ggttgccttc cacgccgcgc acagcaaagc ctttttgatg gcggccgcta tcgctcaagc
     7141 cgacgtcggt ggagtaacgc accaagtctt tgttgtcgcg tatcatttcc tgttgaatgc
     7201 ggccgaggtt gacacgttcc acggttgcag gcgcattaag ctggtcttta acgcgcactt
     7261 cttttatctc tgctttaacg ggtgtggttt cggttgcagc ttcatccgct gccaagaccg
     7321 gattgccgaa aatactgccg accagcgcgg caataggaag catgtgtaat ggtttcatat
     7381 tatcaactca agatgtaatg gattgttcat ccatcagtta attcataata aattttctga
     7441 taatcattaa tatttaataa gacagcaacc cgtacaaata aatctgtgtc gtgtaattaa
     7501 aggtctctgc aaacatctat gccgaggcct tgtttgtttc attattttta agggtctgta
     7561 ccagattagc agatatgtta ccctcgaaat atgaagataa cacactgcaa attaaagaaa
     7621 gaagtacaga aagaaccgct ccgttctttt gtaccggaag ttaccgcccg ttctgccgcc
     7681 gatattttgg gtatccatcc cgattcggcg gcactgtttt accgtaaaat ccgcacggtt
     7741 gccaaccatc gtttggcctt ggctgccgat gaggttttcg agggccccgc cgggccgggt
     7801 gcaagctgtt tcggcgg
//
LOCUS       DBJGGI010000079         7343 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.079, whole genome shotgun sequence.
ACCESSION   DBJGGI010000079 DBJGGI010000000
VERSION     DBJGGI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.079"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            410..856
                     /gene="yciA"
                     /locus_tag="ACSIGJ_001831"
     CDS             410..856
                     /gene="yciA"
                     /locus_tag="ACSIGJ_001831"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HHQ1330642.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVDVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLAGLLPTP"
     gene            complement(907..1728)
                     /locus_tag="ACSIGJ_001832"
     CDS             complement(907..1728)
                     /locus_tag="ACSIGJ_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HHQ1330643.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDERAAGVYEGVEFIRPEDIAETALW
                     LYQRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1998..2456
                     /locus_tag="ACSIGJ_001833"
     CDS             1998..2456
                     /locus_tag="ACSIGJ_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HHQ1330644.1"
                     /translation="MNIQTRLAATALALFLSACGNGGAPAQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQATASFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2621..3736)
                     /gene="lst"
                     /locus_tag="ACSIGJ_001834"
     CDS             complement(2621..3736)
                     /gene="lst"
                     /locus_tag="ACSIGJ_001834"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HHQ1330645.1"
                     /translation="MGLKKVCLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKAMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTINLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            complement(3727..3987)
                     /locus_tag="ACSIGJ_001835"
     CDS             complement(3727..3987)
                     /locus_tag="ACSIGJ_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330646.1"
                     /translation="MAALFALCSILACLNAAAGKEGRNGFPAFGGGVGGVVPDVRRHI
                     FGKIDFNIRQLSEYLLKFRIFRTGTLGAYVQFVGMEFLGIWG"
     gene            4066..6291
                     /locus_tag="ACSIGJ_001836"
     CDS             4066..6291
                     /locus_tag="ACSIGJ_001836"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HHQ1330647.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPGANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWAKDSKTHVATMQSGDFFHNEQSVTVPDATSVSIVFTDKQGNKKELREPVALK
                     AGEIIDAAVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLIANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKDGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGNVLMQHD
                     VEAGDIWRMCQTKDAPIKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKTYLA
                     ELDTDGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFAPLAAALTADEAKIVEELSAVQGKAVDIGGYYAANPEKAAQAMRPSATFNQVLNA
                     L"
     gene            6588..6890
                     /locus_tag="ACSIGJ_001837"
     CDS             6588..6890
                     /locus_tag="ACSIGJ_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330648.1"
                     /translation="MKTVPTILTGILLATALPASAHGMHKSKPLAMDELPPICQQYFK
                     RTETCYNKAGNKADFARNNTKFLFQALPAADLGQRKQMCQIAMDSFAEKTRNLNCE"
     gene            7164..>7343
                     /locus_tag="ACSIGJ_001838"
     CDS             7164..>7343
                     /locus_tag="ACSIGJ_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330649.1"
                     /translation="MTQPEQKRTALNQGGFALRRKRQDSTKFYFIDLNTFISYKNLIY
                     HKNSHIQKSWSNYCIT"
BASE COUNT         1981 a         2153 c         1606 g         1603 t
ORIGIN      
        1 attcccgcgc aggcgggaat ccagacctgt ccgcacagaa acttatcgga taaaaacagt
       61 tgcccaaacc ccgcgttcta gattcccact tccgtgggaa tgacggttca gttgcgttcc
      121 gacaacaccg caatctcgaa acccgtcatt cccgcgcagg cgggaatcca gacctgtccg
      181 cacagaaact tatcggataa aaacagttgc tcaaacccct cgttctagat tcccacttcc
      241 gtgggaatga cggttcagtt gctacggccc caccctacaa tacgccttgc gaatctgttc
      301 cgcgcccgcc cttgtatgtc tcaaatgggt taaactacac acttcccgac ttcccgtttc
      361 gggcaggggt gcaggttcga tttatatttc aataaaacaa ggaaacttta tgcagcacga
      421 agaaggcaac cgccaacgcc ctcaaggcga actgctcctg cgtaccgtcg ctatgccgcg
      481 cgataccaat cccaaccaag acattttcgg cggctggatt atgtcgcaga tggatttggg
      541 cggcggcata ttggcggcgg aaatcgcgcg gggacgcatc gttaccgtcg ccgttcagga
      601 aatgaacttc atccgcccgg tcaaggtcgg caacgtcgtc tgctgctacg ggcattgcgt
      661 ccgcgtgggc aacacttccc tccagcttaa agtcgacgtc tgggtgaaaa ctttgatgaa
      721 cgattgcgtt accgaagacc gctacctcgt aaccgaagcc gtgttcactt atgttgccat
      781 cgatgcggaa ggcaatccgc gcccgattcc gaaagaaggc aaccccaaat tggcgggctt
      841 attgcctact ccgtaaaaat acccgtaaaa atgccgtctg aaactgtctt cagacggcat
      901 tttgcatcat ttgaaccagg acctgatgcg ggaaaacagc gacatactct gtttttcaaa
      961 gtcttcccgc acttcgggca cggctttttt tatcactttc attcctgcaa aagtctgcgc
     1021 cacgggcata atttcaatcg tgttcacatt catatgcgcc ggccgctggt acagccacaa
     1081 tgcggtttcc gcaatatctt cggggcggat aaattccaca ccctcataca cgcccgccgc
     1141 cctctcgtca tcgcctttga agcgcacatt ggaaaactcc gtattgccgc acaaacccgg
     1201 ctcgatattg gtaacgcgga tgttcttatc cgccaactcc gcgcgcaaat tcaggctgaa
     1261 ctgccgcaca aacgccttgg tcgccccgta aacgttgctg ccggcataag cataattgcc
     1321 tgcaatcgaa cccaaattca tcacatagcc gccgccgcgt tccaccattt gcggcaaaat
     1381 tttgcgcgtc aggaacgtca aacccaaaac attggtttga atcatcgttt cccaatcttc
     1441 aaaatccgcc ttgtccgccg tgtccagacc caaagccagc ccggcattgt tgatgaggca
     1501 gtcgatgtcg gaaaattcat cggggatgcc gtttaaggcg ttttccaccg actcgcgtcg
     1561 cgacacgtcc atttccaaag ggtaaaacaa agcacccaat tcatccgcca aggcctgaag
     1621 cctgtccgca cggcgcgccg caccgataac gcggtatccc gccccgacaa acgcgcggca
     1681 catcgcttcg ccgaatcctg ccgaagcacc ggtaattaaa acagccattg tttttccttt
     1741 ctttctcgtt tcccaaacct gtttcaggta tcataacgct cttcagacgg catcgcgccg
     1801 gctgaaaatg acaacaacca taccaccacg acaggacaca ttcaatatgt ttcaacacac
     1861 aggacggcac ataaagcgtc gccctatgtg ccgtcctgat tcggaaggga ttgcaccctt
     1921 cccaaataaa atctgattct accgccccga aggacggatg tccaagtggg cggggtttca
     1981 accgaaaagg aaacacgatg aatattcaaa cccgccttgc cgcaaccgcc ctcgccctat
     2041 tcctttccgc ctgcggaaac ggcggcgcgc ccgcacagcc caaaggtgaa atttccgaaa
     2101 accgcaccgc cgcgttcaaa tctatgatgc ccgacttctc gcgtatgggc aaaatggtca
     2161 aaggcgaaga gccttacgat gtcgaaaaat tcaaacaggc gacagcgtcg tttgccgaaa
     2221 gcagcaagaa accgttcaca ctttttgagt ccgatccgca aggcaacggc cgcgccctgc
     2281 ccgccgtttg gtcggatggt gcaaaatttg aagccgaaaa aacaaaattc gccgccgccg
     2341 tcgaaaaact caacgccgct gcccaaaccg gcaaactgga tgaaatcaaa gccgcctacg
     2401 gcgaaaccgg cgcaagctgc aaatcctgcc acgacagttt ccgcgcgccc gaataacatc
     2461 caaaacaaaa caatgccgtc tgaagccttc agacggcatt ttctttgcca tttcagcacg
     2521 cgcaaagcaa tcggaaatta tcgtcaatcc aaatgaaaaa acgctatcct tatttttacc
     2581 ccacgcactc cgccattttc tattttgatt tgctatgcga ttaatcttta tcgtcaaatg
     2641 tcaaaatcgg gatgccggat tgggtaaaca gggcatacac cggcttgagc caataatctt
     2701 ccggaaggga agccggtttc aatgcgtaaa cgtgcacatt gggaaaatcc ttcatcgtca
     2761 acgccgcacc gctgaaaaag gtataaattt catacctcgt atgcgggttt ttcttaattt
     2821 cgcgcaaaat atagtcttcg atgacatagg gcgaatttaa tgtggttacg ccggaaagcc
     2881 cgtaggtttg gcgggggtgc ggtgcgacat attgtatgtt gaagtttttt gccgcctttt
     2941 ccgaaatttc cttcatctcc ttgtcgggcg aacccaaaag tatccgcacc gtgccgcccg
     3001 tttcgtcccc cgccttcagt tcggacgcat cgaacagcgg caggtaagtc atcttgcggc
     3061 ggccgtcgtc cataatgttt ttcaaaccct tgaatatcgt gtaatgctcg tcggaagcat
     3121 tacgggtttt ggcgatgctc caatctccga tcatcatccg ggcaaaattc cgcttgatcg
     3181 tcccgtttac agaaaactca tcacccaaat agctgctgct ttgaattaaa ttgattgtcc
     3241 cgtcgtcaaa ggttttgatt tccgcatccg ggtaagtgct caaaaaggcg gcaatgctga
     3301 ctttttccaa acttgccaaa taaatccgct tgaccttcgg cagcagcatt gccttcacct
     3361 tcagctccgc catcgtcgga atgaaattaa acgatttgtt caaaccgtag ggcaggtgga
     3421 aaaaatacgc ccgctccgcc ttatccttta tctgattgaa ataataatcg tatttttcat
     3481 tcctgttctc agacatcagc accacataaa accgctcccc cggatgctgc gccataatcc
     3541 tttccgccac cttcatctgc aatatggtat agcagaaaat cagattgacc ggttccccct
     3601 cttcattgaa gagtttgtcc ttcagcaggg aaaccgcatt cctttccccc tgatttaccc
     3661 ggtcaaatgt ataaaatatc ccgaaacaaa aaacaatcag gcacaacacg gtcaaacaga
     3721 cttttttcaa ccccatatcc ctaaaaactc cattccgaca aattgaacat acgccccgag
     3781 tgtcccggtg cggaaaatgc ggaattttag cagatattcc gacagttgcc gaatattaaa
     3841 atcaatttta ccgaaaatgt gccgccgcac atcaggcaca acgccgccga cgccgccgcc
     3901 aaacgcggga aagccatttc tcccttcctt tcccgccgcc gcgttcaaac acgccaagat
     3961 cgaacaaagc gcgaacaaag ccgccaaaat atgccataat agccgcgcac attccccgcg
     4021 caatgtgttt caaaagaaaa aaccaaccta caagagaata atcctatgac tcaaaaatcc
     4081 accattgttt atacccatac cgacgaagcc cccgcgctgg cgacccaatc gctgctgccg
     4141 attgtgcagg cgtttgcccg ccacgccgat attgatgtca aaactgccga catttccctg
     4201 tccggccgca ttttggcggc attccccgaa tatctgaccg aagcgcagcg cgtacccgac
     4261 gcgcttgccg aattgggcga actggtgaaa caacccggtg caaacgtaat caaactgccg
     4321 aacatcagcg catccgtacc tcaactgacc gccgcgatta aagaattgca gtctaaaggc
     4381 tttgccgttc ccgactatcc tgccgaccct caaaccgatg aagaaaaagc cgtacgcgaa
     4441 cgctacgacc gcatcaaagg cagcgcggta aaccctgtcc tgcgcgaagg caactccgac
     4501 cgccgcgcgc ctaaagcggt gaaaaacttt gccaaaaaaa atccgcacag catgggcgcg
     4561 tgggcgaaag actccaaaac ccacgttgcc accatgcaaa gcggcgactt tttccataac
     4621 gaacaatccg ttaccgtacc cgatgcgact tccgtatcca tcgtgttcac cgacaaacaa
     4681 ggcaataaaa aagagctgcg cgagcctgtt gccctgaaag ccggcgaaat cattgacgcg
     4741 gccgtaatga gcaaaaaagc cctgctcgcc ttccttgccg aacaagtgaa agacgcaaaa
     4801 gctaaaggcg tattgttctc gctgcatatg aaagccacga tgatgaaagt gtccgacccg
     4861 attatcttcg gacacgccgt caaagtgttc ttcgcgcctg tatttgaaaa attcggcggc
     4921 aaactggctg ccgcaggcgt caacgtcaac aacggcttcg gcaacctgat tgccaatctg
     4981 gacaagctgg atgcggacac ccgcgccgcc gtcgaagctg aaatcgccgc cgtttacgcc
     5041 gccaaccctg atttggcgat ggtcgattcc gacaaaggca tcaccaacct gcacgttccc
     5101 agcgatgtca tcgttgatgc ttctatgccc gcgatgattc gtaattccgg ccgtatgtgg
     5161 gacaaagacg gcaaagcgca agacaccaaa gccgtgattc ccgacagcag ctatgccggt
     5221 gtttaccaag caaccatcga cttctgccgc gaacacggcg cattcgatcc gacaaccatg
     5281 ggtaccgtcc ctaacgtcgg cctgatggcg caagcagccg aagaatacgg ttcgcacaac
     5341 aaaactttcg aaatcgaagc cgacggccaa gtccaagtca tcgatgcagc aggtaatgtc
     5401 ctgatgcaac acgacgttga agccggcgac atctggcgta tgtgccaaac caaagacgcg
     5461 cccatcaaag actgggtaca actcgccgtc aaccgcgccc gtctgagcaa tacaccagcc
     5521 gtgttctggc tcgacgaaaa ccgtccgcac gacaagagcc tgctcgccaa ggttaaaacc
     5581 taccttgccg aactggatac cgacggcctc gacatccgcg tcctcgcgcc cgaagaagcc
     5641 gccaagttca gcttaggccg tctgaaaaac ggcgaagaca ccatctccgt taccggcaac
     5701 gtcttgcgcg actacctgac cgacttgttc ccaattttgg aactcggcac cagtgcgaaa
     5761 atgctgtcta tcgttccttt gatgaacggc ggcggtatgt ttgaaaccgg tgcgggcggt
     5821 tctgcaccaa aacacgtcca acagttcctc gaagaaaacc acctgcgttg ggactcattg
     5881 ggcgaattcc tcgcgcttgc cgtatcgttt gaacatctgg cgcaaaaaac cggcaacgcc
     5941 aaagcccaag tcctcgccga cactttggat gccgccaccg aaaaactgct gttgaacgac
     6001 aaatcgccca aacgcaaagc gggcgaactc gacaaccgcg gcagccattt ctacctcacc
     6061 ctctactggg cgcaagaatt ggcggcgcaa gacaaagatg ccgaactgaa agccgcattt
     6121 gctccattgg cagccgcttt gactgccgac gaagccaaaa tcgttgaaga actctctgcc
     6181 gtacaaggca aagcggtcga catcggcggc tactacgcag ccaatcctga aaaagccgca
     6241 caagcgatgc gtccgagcgc aacctttaat caagtactaa acgccttata agcacaaagg
     6301 caaaaatgcc gtctgaacat tcgtcgttca gacggcatta gttatcccta tctgcctgat
     6361 tatgattaaa cgccgcaccc attggatata tagtggatta acaaaaacca gtacggcgtt
     6421 gccccgcctt agctcaaaga gaacgattct ctaaggtgct gaagcaccaa gcgaaccggt
     6481 tccgtactat ttgtactgtc tgcggcttcg tcaccttgtc ctgatttttg ttaatccact
     6541 ataaatcata taatctttag ttttttaccc tacggaaaga caataatatg aaaactgttc
     6601 caaccatcct gaccggcatc cttttggcaa ccgcccttcc cgcatccgca cacggtatgc
     6661 ataagagcaa acccctggct atggacgagc tgccgccgat ttgccaacaa tatttcaaac
     6721 gcaccgaaac ctgttacaac aaagccggaa acaaagcaga tttcgcacgc aacaacacca
     6781 aattcctatt ccaagcccta cctgccgccg atttgggaca gcgtaagcaa atgtgccaaa
     6841 tcgctatgga ttcgtttgca gaaaaaaccc gcaatctgaa ttgcgaataa agccgcatca
     6901 gcaatagcaa tgccgtctga aacaaaggtt cagacggcat tctcacatca aaacgtaccg
     6961 gctgataaaa cccgccttcc ccgcatccct gacccgaaca ggattaaacg gagtaacggt
     7021 ttcggccatt atggcaagtc cccatcccaa aaatgaccat ataaattaat accataaaac
     7081 attgataaaa agcgtctgcc ggttgcggtt tgttgcaggg cgttacggaa aatcatcgta
     7141 aaccgatgat ttttatatag taattgacac aacctgaaca aaaaagaacc gccctgaatc
     7201 agggcggttt tgctttgcgg cggaaacggc aggattcgac taaattttat tttattgatt
     7261 taaatacatt tatttcttat aaaaatttaa tttaccataa aaacagccat atacaaaaat
     7321 cttggagtaa ctactgcatt aca
//
LOCUS       DBJGGI010000080         7325 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.080, whole genome shotgun sequence.
ACCESSION   DBJGGI010000080 DBJGGI010000000
VERSION     DBJGGI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.080"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(<1..262)
                     /locus_tag="ACSIGJ_001839"
     CDS             complement(<1..262)
                     /locus_tag="ACSIGJ_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330650.1"
                     /translation="MPSLQRRSRGEKSSCIKQLNGGNYPPFLICCQLFFENLPDRFKF
                     CKKLARWLSGNKFPQKPVNPIIQKIICFHRVSFSVETLPFRAV"
     gene            complement(571..1035)
                     /locus_tag="ACSIGJ_001840"
     CDS             complement(571..1035)
                     /locus_tag="ACSIGJ_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HHQ1330651.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSSTPVTERVCRL
                     EREHYISGYHAHLNPHLLGKPPLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
     gene            1257..2315
                     /gene="alr"
                     /locus_tag="ACSIGJ_001841"
     CDS             1257..2315
                     /gene="alr"
                     /locus_tag="ACSIGJ_001841"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HHQ1330652.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTKGLEGEESLANSAAILNIPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRISMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(2857..4212)
                     /locus_tag="ACSIGJ_001842"
     CDS             complement(2857..4212)
                     /locus_tag="ACSIGJ_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HHQ1330653.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKHLSAKYGVPIVNQRISVTPIAQIAAATKADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSVPEAMKTTDIVCSSINIGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDAVSLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPAKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(4227..4499)
                     /locus_tag="ACSIGJ_001843"
     CDS             complement(4227..4499)
                     /locus_tag="ACSIGJ_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HHQ1330654.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEILDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(4624..5361)
                     /locus_tag="ACSIGJ_001844"
     CDS             complement(4624..5361)
                     /locus_tag="ACSIGJ_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HHQ1330655.1"
                     /translation="MKKLSRIVFSIVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAAGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(5499..6398)
                     /gene="prmB"
                     /locus_tag="ACSIGJ_001845"
     CDS             complement(5499..6398)
                     /gene="prmB"
                     /locus_tag="ACSIGJ_001845"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HHQ1330656.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAVYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIVPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINIEDYGLEERIQLIHTDLFEGLEGTYDLIVSNPPYVDAESVGALPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            complement(6497..6694)
                     /locus_tag="ACSIGJ_001846"
     CDS             complement(6497..6694)
                     /locus_tag="ACSIGJ_001846"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330657.1"
                     /translation="MGERLVQTDYAHPAVGIADIGHLVVFCGYPLLALNKPRFFPAYQ
                     VAHTVRHRRFRSGNGLRAAFM"
     gene            6730..7215
                     /locus_tag="ACSIGJ_001847"
     CDS             6730..7215
                     /locus_tag="ACSIGJ_001847"
                     /inference="COORDINATES: protein motif:HMM:NF037911.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330658.1"
                     /translation="MRGCSRCRADRQAGERQPRDGNGRWTAGEVVSVSGGELGEFAET
                     KELRKAAMQYARDNFVGKSYVNESSGHELKVTWQGVKHAASKSNSIELSLMVALDKVL
                     MNAKYEGSFPNKLGRDHIIAVHKYSTGVRFGNKVLKIRIIAREKFDGIKHYDHFILKD
                     K"
BASE COUNT         1973 a         1783 c         1671 g         1898 t
ORIGIN      
        1 ctaccgccct aaagggcagg gtttcaaccg aaaaggaaac acgatgaaag catatgattt
       61 tttgaataat cggattgacg ggtttttgag ggaatttgtt accggatagc catcgggcaa
      121 gttttttgca aaatttgaat cggtcgggta aattttcaaa aaataattga cagcagataa
      181 gaaacggcgg ataattaccg ccgttgagtt gcttgatgca gcttgatttt tctcctctac
      241 ttctcctttg tagacttggc acacattcaa ccggatgtgt gcatttttta tctccgcttt
      301 ttttgaaatt taattacttt tattgtttga aattctattc tttaagaaat ttaacaagaa
      361 aaacgcttgc tttttgaccg gaaaagctgc ataattcgtt ccgttagctg gttcgagtag
      421 tcagttaata gtttctcctc tatttctcct ttgtagactt ggcacacatt ctattgaatg
      481 tgtgcatttt ttatctgaag caacaagcct ctgtgcgtga tgttgttatg tttcatttag
      541 atgtcaaacc gcatacccgg tctgaaatat tcaatccaaa tccaaaaccg gattttcttt
      601 gacttcctcc atcacaacat aactcctact ctccgaagcg gcaggcagtt gcaataggat
      661 attgccgagc atatcccgat aggcagacat atcgggcaaa cgtactttaa tcaggtagtc
      721 gtattcgccc gacaccagat ggcactccat aatttgcgga attttcagca cttctttttt
      781 gaaatcttcg aaaatattgc ccgatttgga ttgcagcttc agctcgacaa aaaccaatgg
      841 cggttttccc aacagatggg gattgagatg ggcgtgatag ccggaaatat aatgttcccg
      901 ctccaaacgg cataccctct ctgtaacggg cgtgctggac aaccctacct tctcggcaag
      961 ctccgtcatc gggatacggg cattttgctg aagaattttg aggatgcgga aatcgatttt
     1021 atctagttct ttcatttgga ttaccttgta tttattattg attttaacaa atagagtata
     1081 aatttgggaa agcaattata tcaggaaaag caaaccgcct tcctacctga aaactgctgc
     1141 tctggcttga agacacaagg ttctttaata ttttaaaagc cttgccgttg gattataatt
     1201 ccccaattga tttcttaatt ttgctaataa acacttgttt ggtaaggaat gaatttatgc
     1261 ggcctttgaa cgtgcagatc aggttgggca accttaggca caattatcgg attttgaagg
     1321 aaatgcacgg aggcaagctg ttggcggtag tgaaggccga cgcatacgga cacggtgcgg
     1381 tcagatgtgc tttcgcgctg gcagacttgg cagacggctt tgccgtggcg acaatcgacg
     1441 agggaatcag gctgcgggag agcggcatta cccatccgat tgtcctttta gaaggcgtat
     1501 ttgaagcatc agagtacgaa gcggtcgaac aatactcgct ttggccggca gtcggaaacc
     1561 aatggcagct tgaggctttg ctttcccgcc attggaaaaa acctgtcaag gtctggttga
     1621 aaatggattc gggaatgcac cgtaccggat ttttccctca tgattacgct tcggcatatg
     1681 cggcattgaa acagtcggaa tatgtggaca gtattgtcaa gttctcgcat ttctcctgtg
     1741 cggacgaacc tgaaagcggt atgacggaaa tacagatgga agcattcgat ttgggtacga
     1801 aagggctgga aggcgaagaa agccttgcca attcggcagc tattttgaat atccccgaag
     1861 cacgcaggga ttgggggcgc gcgggcttgg cgttatacgg catttccccg ttcggaggca
     1921 gcgatgacag gctgaaaccc gtgatgaggc tttcaacccg tattttcggc gaacgcgttt
     1981 tacagccgca ctcccctatc ggttatggcg caacatttta taccagtaaa tctacgcgcg
     2041 tcggtctgat tgcctgcggt tatgcggacg gttatccgcg ccgcgctcca agcaattccc
     2101 ccgtcgccgt cgacggtaaa ttgacccggg tcatcggcag gatctctatg gatatgatga
     2161 ctatcgagct ggacgcttcg caagaaggtt tgggacacga ggtcgaactg tggggcgata
     2221 cggtcaatat caataccgtt gccgaagcgg ccggaaccat cccttacgaa ttgatgtgca
     2281 atatcaaacg tgcaaaattc acttatatcg agtaatcgag tccaaacgaa aatcccgtct
     2341 gaagcttttc agacggcata aatgcgtttt gacaaaatca gtaaaagaca ttgccaaata
     2401 gaaaatactg ataatgccca caatctgaaa ccccatcctt cccgcaaaaa cggaaaatca
     2461 aaatcagaaa cctaaaattt cgtcattccc gcgcaggcgg gaatccggtt ttttgagttt
     2521 ccgtcatttc tgataaattc tcgtcgtttt tcatttctag attcccgcat gcgcgggaat
     2581 gacaaaatta aagtttcaga atttatttga acaacaaact agattcccgc tttcgtgggg
     2641 atgacggcgg ataggttttg ttgtttcgga taaattcctg ttgttttttg gtttccagat
     2701 tcccaccttt aaaagaatga cgtaagaaag cagaaataag aacacgggga ttttttaaat
     2761 ttgcaatatt cttttctaaa gacattttac ccaatgccca taaacaaaaa tgccgcctga
     2821 agcctttcag acggcatttt cccatcaaaa ccgcaatcag tttttcatcg attgaaccgg
     2881 tgcgggaatc ctgccgcccc ggttgacgaa cacttcgcac gaaccttctt ttgccggcat
     2941 tacaggcgcg tagcccaaca gaccgccgaa ctcgacgctg tcgccgacgg ttttgcccgt
     3001 taccggaata atgcgcacgg cggtggtttt gctgttgatc atgccgatgg cggcttcgtc
     3061 ggcgatgatg ccggaaatgg tgtgcgcggg cgtgtcgccg ggaacggcaa tcatgtccaa
     3121 accaacggag cagacggcgg tcatggcttc gagtttgtcc agcgtcaaca cgcctgcctc
     3181 ggcggcggca atcatacctt cgtcttcgga aacggggata aacgcgccgc tcaaaccgcc
     3241 gaccgcgctg gaagccatca tgccgccctt tttcacggca tcgttcagca atgccaaagc
     3301 tgctgttgtg ccgtgcgtac cgcagacgct caagcccatt tcttcaagaa tgcgcgccac
     3361 cgagtcgccg acggcggggg tcggtgccag cgacaaatcg agaatgccga acgggatatt
     3421 cagcattttt gaggcttcgc ggccgatgag ttcgcccacg cgggtgattt tgaaagcggt
     3481 tttcttcacg acttcggcga cctcggtcag gctgaccgcg tccgaatttt ccagcgcggc
     3541 tttgaccacg cctggaccgg atacgccgac attaatcaca gcatccgctt cgcccgagcc
     3601 gtggaacgca cccgccataa acggattgtc ttccaccgcg ttgcagaaca cgacgatttt
     3661 ggcgcagccg aaaccttcgg gcgtgatttc agccgtgcgt ttgatggttt cgcctgccag
     3721 cttgaccgca tccatattga taccggcacg cgtgctgccg atattgatgg agctgcacac
     3781 gatatcggta gttttcatcg cttcgggaac ggaacggatc aacacctcat ccgaaggcga
     3841 catacctttt tgcaccagcg cggaaaagcc gccgataaag gacacgccga tggctttggc
     3901 tgccttgtcc aaagtctgcg ccacgctgac ataactgtcg gctttggtcg ccgccgcgat
     3961 ttgggcaatc ggcgtaacgg aaatgcgctg attcacaatc ggcacgccgt atttggcgga
     4021 aaggtgtttc gccgttgcca ccaagtcttt gccgaccgtg gtgattttgt tgtaaatgtt
     4081 ttggtttaac acgtcgatgt cggtgctgat gcagtcgtgc aaatcaatgc cgatggtaat
     4141 ggtgcggaca tcaaaattct ggtcggcaac cattttgacg gtttctaaaa tttcgccgga
     4201 ttggatactc atcacattcc tccgactcaa atgcggtgca tcgcttggaa gatttcttcg
     4261 ttttgcatac ggatgtcaag cgcgagtttt ttgctctctt ccgcaaacaa atccaaaatc
     4321 tcctgccgcg atttagggca tttcgaggta tccaccaaga taatcatagt aaaaaaatcg
     4381 tccatcagct gttggctgat attgaggatg ttgattcggt tttccgccaa aattttggaa
     4441 acatcgtaca cgatgccgac gcggtcttta ccgatgacgg tgatgactga attgttcaca
     4501 ggcttactcc ttgcagatat ccgttaaaat ccgaaattat accaccgtcg gattttgaag
     4561 aatattgtca acaatatata catacaaaat gccgtctgaa actatttcag acagcatcaa
     4621 gattcagggt tcgattaaat aaccatcctt atcccattgg gttttcctga ccaacttgtc
     4681 atcctgataa acagcctcgc tctttttaga accatcttca taccactcca aaaccacccc
     4741 gttgcgttta tggtggcgga tagacagttc cgaaagtaat cggccgcttt catcccaagt
     4801 cagaattttg gcaggctcat cgttgaccat aaccatttcc gtcttgatac ttccatcggc
     4861 ataccattgc ttccatacgc cgttcgcctt attttgctta aactggattt cgctttcctt
     4921 gccgccgtta cggtaataac ggtatcccgt accctcactc aagccatttt tataaggcat
     4981 aaccgcagat tttttaccgt tcggatacca gttgacccat tccccgtccg gcttaccctt
     5041 gctgaagccc cccgccattt ttttctgacc attaaaatgc cacaaaatca acataccgtt
     5101 ttgcagggta ggcacaaaag atttgatttg cgttgaagca acgatataag gttcggaata
     5161 tttcttcatc gacggataat aaaaatcctg cgcgtgtgcg atacccgccg ccacactata
     5221 ttgcctgata taagcggcag aagacatcgt cgccgtcagt ttcccgttct gattaaaata
     5281 aacagaatag gtctgcgccg gcaaagcggc cgaaaaaccc aacagtacga ttgaaaatac
     5341 aatccgagat aatttcttca ttatgatagc gatataaaaa acaattaact tataaacaat
     5401 atggtaagga tttctcaacc aagcgtcaaa cccgaaacaa tgtatcgtaa aaatgccgtc
     5461 tgaaaacaaa tcgtcttcag acggcatttc cctccaactc actcttcgcc caataactgc
     5521 tcgcgcgtca agaggaaaac aaaaccgtcg cccccgctgg tttccaacca agtaaaaggc
     5581 aactccggat acgctgcttc caatacatcc ctgttatgcc cgatttccac cagcaataca
     5641 cctttaggat tcagaaactt tgccgcattc agaagaatct gcctggtggc atccaacccg
     5701 tccgccccgc tgcccaacgc caattccggt tcgtgcaaat attcctcagg caaagcccca
     5761 accgactctg catccacata aggcggattg gaaacaatca aatcataagt gccttccagt
     5821 ccctcaaaca aatcggtatg aataagctga atacgctctt ccagaccata atcttcaata
     5881 ttgatccctg caacttccaa agcatccaaa ctcacatcaa ccgcatcaat ttgggcatca
     5941 ggataatggt gcgccatctg aatggcaagg caaccgcttc cggtgcaaag atccaaagca
     6001 ttatgcacca gctcatcgta ttctatccaa ggtcgaagtc cgtcacccag caattcataa
     6061 ataaaagagc gcggtacaat cacgcgttca tctacataaa aatcaaactc cccctgccag
     6121 gcctggtgtg tcaaataagc ggcaggaatg tgttcgacag cacgacgctc aataaccgcc
     6181 agcacttcct ctttttcagc ttccaagagt tttgcatcaa gatatggggc aagcatatcc
     6241 aaaggcaaat tcaaagtatg cagaatcaaa taaaccgctt catcatgcgc attatctgtt
     6301 ccatgaccaa aaaagagccc tgcctcatta aaacggctga ctgcaaaacg taaaacatca
     6361 cggatagtcg tcaattcttg tgcggcctga ttaaacataa tatgaaccat tctgcgtata
     6421 gatgctttta attataacag aaacaacaag caaacctttt catatcgcca aataaccact
     6481 caatctaccc atacaactac ataaatgccg cgcgaagacc attgcccgaa cggaaacggc
     6541 gatggcggac ggtatgggca acctgatagg ctgggaagaa acggggcttg ttgagggcaa
     6601 gcagtggata accgcaaaag acgacaaggt gtccgatgtc tgcaatgcca acggcgggat
     6661 gggcgtaatc ggtttgcacg agcctttctc ccacggtgcg ttgacgatac ccgagcatcc
     6721 aaatggcagg tgcgcggttg ttcccgttgt cgggcggaca ggcaagctgg cgaaaggcag
     6781 ccgcgcgatg gaaacgggcg atggacggca ggtgaggttg tttccgtatc gggcggcgaa
     6841 ttgggggaat ttgctgaaac aaaggagctt cgtaaagcgg ctatgcagta tgcgcgtgat
     6901 aactttgtcg gcaaaagcta tgtcaatgaa agcagcgggc atgaactgaa ggtaacttgg
     6961 caaggtgtga aacacgctgc gtcaaaatcg aacagcatcg aactttccct tatggtcgcg
     7021 cttgataaag ttttgatgaa tgcaaaatat gaaggaagtt ttcctaacaa attagggagg
     7081 gatcatatta ttgcggtaca caaatactca accggtgtca gatttggcaa taaagtctta
     7141 aaaatcagga taatagcccg tgagaaattt gatgggataa aacattacga tcattttatt
     7201 ttgaaagaca aatgaaaaaa ccctgtccag tatatctggg atagcttatg gaagcattta
     7261 acccagcctc ggataaggct ttaaatccat catgccattt atacggtaaa aacaagggtc
     7321 tgtac
//
LOCUS       DBJGGI010000081         7228 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.081, whole genome shotgun sequence.
ACCESSION   DBJGGI010000081 DBJGGI010000000
VERSION     DBJGGI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.081"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            143..292
                     /locus_tag="ACSIGJ_001848"
     CDS             143..292
                     /locus_tag="ACSIGJ_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330659.1"
                     /translation="MNAEGFCEKQTACRPDGRRNAGDRADLKAFEFAGRELANYREML
                     KRYAV"
     gene            322..3369
                     /locus_tag="ACSIGJ_001849"
     CDS             322..3369
                     /locus_tag="ACSIGJ_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HHQ1330660.1"
                     /translation="MSDLEAKVRITVENHTKQGFDSAAADADKAAEKMRSSGDNAAKG
                     FKAAIDSMHETMRNLHADVKAGFEAAGNQAQQASEKVRAEVGKIGSGLSGLTKLLAGL
                     ATADFAKSVLDTADAMQSINSQVRQVTSSETEYLAVQQQLLDTANRTCASLESTANLY
                     VSTSRALKDYGYTQQEILKFTEATNNAMTIGGVGARQQAAALFQLSQALGSGVLQGDE
                     FKSISEAAPILLDTIAEYMGKSRDEIKKLGSEGKLTADVIFKAISGASEKFGEQAAKM
                     PVTMGQALTVFSNNWQSMVSKLLNDSGTMSGIAAVIKLIADNLNLVVPIVAGFAVAVA
                     AAVAPTLALNLALLANPFGIIAVAIGTVVGLIAKFGDEIDVFGGGWSNLSDVIRAVWQ
                     IITETVGEAVGTVKSWFDGLTGRLNEGAGGWPSLFGRVMSVISSAIGAYVNVYINIFA
                     TGWMLIKEAANDMPQFFANLGKAIGNVFISAIERMINKAVGMINSMIDFANKAASMAG
                     ISGIGKLNKVRMGRMDDGGLGERIHDSLTKDRAGAMANAVRERAADIHEADALKGRGG
                     GGHAKTARKKPGANQGGGKGGKSRSGGPGAAKDPMQAWEGEIKARKLAHREMQRETLT
                     HQEWDLAREAEYWRAKLATVDANGKTGVKIREKILTLEDQLSKQSTEAKMNQAAEWEK
                     LDKHKLEMEKDAADQALADGRISQLERLDLEIEFENRRYQIAYDALQERIALAEQDPA
                     YSQAAIGKLKAQMGELGRGHERTQAKNEGKRENQRRKDAPNVMEMLQDGGRNVWQEAQ
                     QQMGQAFTAMLARAQNFRQAMNGFFKSMGQTFIQEMVTKPLTGLMRRMVEESAIYKMI
                     FGTKETLETAAAAKTAATKATETTAVVSSNAVQAASGAAASQAHIPYVGPILAVAAMA
                     AMMSAVMGLMGGGGGSQTTTTTTRIPSAAGGWDIPAGINPLTQLHENEMVLPAEHAQT
                     IREMADQQGGGSTVIINSTGGDFIHKNDLAKLLKQMKRDFKFV"
     gene            complement(3429..3908)
                     /locus_tag="ACSIGJ_001850"
     CDS             complement(3429..3908)
                     /locus_tag="ACSIGJ_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HHQ1330661.1"
                     /translation="MTDNLIQIATPILTVIGVFVAAYGIMRNTENAKKRATIDMIMAE
                     RNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAILTVLNQREFVCAGVLGG
                     ALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQEFEAVARKWKKKPLKTK"
     gene            complement(4250..5239)
                     /locus_tag="ACSIGJ_001851"
     CDS             complement(4250..5239)
                     /locus_tag="ACSIGJ_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHQ1330662.1"
                     /translation="MATITQRNGKWRVQIRMKGVSRSATFERASDAKAWAARIESQIM
                     DGIQGNAPRNTIFADLIRRYLSEVTPSKRGAREESYRIGRALKTPLAKVRLADLRPQD
                     FADWRDQRLQEVSPTSVGRELTTLSAVCEHAMKEWGLLRENPVRKISKPKKSRARTRR
                     PTEQEIADICAALLYRPNEKPKMAVQRVAVAVLFAIETAMRAGEICGLKWADVNMRRR
                     IAHLPITKNGDSRDVPLSLRAAELIEQLRGIDDTWVFSLDAKSLDVLFRRARDNCGIQ
                     GLHFHDTRREALTRLSKKVPVEVLAKISGHRDLRILLNVYYRPDMADIAKMLD"
     gene            complement(5499..5687)
                     /locus_tag="ACSIGJ_001852"
     CDS             complement(5499..5687)
                     /locus_tag="ACSIGJ_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330663.1"
                     /translation="MADGRKGFFDAIIKINIFFFPVKTPTLGAANPIAGGSTRFRFGA
                     EQMAVLYVPFCVLKHIGR"
     gene            5928..6962
                     /gene="purM"
                     /locus_tag="ACSIGJ_001853"
     CDS             5928..6962
                     /gene="purM"
                     /locus_tag="ACSIGJ_001853"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HHQ1330664.1"
                     /translation="MSTSLSYRDAGVGIDAGDQLVEKIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYQNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPKNTVAQIDAESWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVRSFLSGQGETVYRLGCIRER
                     QGNEHQTQVA"
BASE COUNT         1818 a         1749 c         2003 g         1658 t
ORIGIN      
        1 atccggacaa caaccgatgc cgcaccctgc gggcaggctt cgcactctga aaggttcagg
       61 cggcatttgt attccctgcg cgtcaaaccg ccttcgtgta aagcggttgc aaaaaagcct
      121 tttattttga cagggtgcag agatgaacgc cgaaggattt tgtgaaaaac aaaccgcctg
      181 ccggccggac gggcgccgaa atgcgggcga tcgtgccgat ttgaaggcct ttgagtttgc
      241 cggacgggaa ttggcgaatt atcgggaaat gttgaaacgt tatgccgtct gaaaagacgg
      301 cttttttatt tggggctgta aatgagcgat ttggaagcca aagtcaggat aacggtagaa
      361 aaccatacga agcagggttt tgattcggcc gccgccgatg ctgataaggc ggcggaaaag
      421 atgcgcagca gcggcgataa tgccgcaaag ggctttaaag ctgcaatcga cagtatgcac
      481 gagaccatgc gcaatttgca cgccgatgtg aaagcgggat ttgaagcggc gggcaatcag
      541 gcgcaacagg cgtctgaaaa ggttagggcc gaagtgggta aaatcggctc aggcttatcg
      601 gggctgacca aactgctggc aggattagca accgcagact tcgccaaatc ggtgcttgat
      661 accgccgatg cgatgcagtc gataaacagc caagtccgac aagtcacgtc gtctgaaacg
      721 gagtatctgg ccgtgcagca gcagcttctc gatacggcaa accgtacctg tgcatcgctt
      781 gaatcaacgg caaacctgta cgtttccaca agccgcgcgt tgaaagacta cggctacacg
      841 cagcaggaaa ttttaaaatt caccgaggca accaacaacg cgatgaccat cggcggcgtt
      901 ggcgcgcgac aacaggccgc cgcgctgttc cagttatcgc aggctttggg cagcggcgta
      961 ttgcagggcg atgaatttaa atccatttcc gaagccgcac ctattttgct ggataccatt
     1021 gcggaatata tgggcaaatc ccgcgacgaa atcaaaaagc tgggcagcga agggaaattg
     1081 acggcggatg tgattttcaa agccatttcc ggcgcgtcgg agaagttcgg ggagcaggcg
     1141 gccaaaatgc ccgtgacgat ggggcaggct ttgacggtgt tctcgaacaa ctggcaaagc
     1201 atggtttcaa aactgctgaa cgacagcggc acaatgtcgg ggattgccgc cgttattaaa
     1261 ctgattgcgg ataaccttaa tttggttgtt ccgatcgtcg caggttttgc cgttgccgtt
     1321 gccgccgccg ttgcaccgac actggctttg aatcttgcac tgctggcaaa cccgttcggg
     1381 attattgccg tcgcaatcgg cacggttgtc gggctgattg cgaaattcgg cgatgaaatc
     1441 gatgttttcg gcggcggctg gtcgaatctt tccgatgtga ttcgggcggt ttggcaaatc
     1501 atcacggaaa ccgtcgggga agcggtggga accgtcaaat cgtggtttga cgggctgaca
     1561 ggccggctga acgagggcgc gggcggctgg ccgtcgttgt tcgggcgcgt gatgagcgtc
     1621 atctcaagcg caatcggcgc gtatgtgaac gtttatatca acatattcgc aaccggctgg
     1681 atgctgatta aagaagccgc aaacgatatg ccgcaattct tcgccaatct cggcaaggcc
     1741 ataggcaacg tgtttatttc cgcgatcgaa cggatgataa acaaagcggt aggcatgatt
     1801 aacagcatga ttgactttgc caacaaagcc gcgtcgatgg ccgggatatc gggcattgga
     1861 aagctgaaca aggttcggat ggggcggatg gacgacggcg ggcttggcga gcgtattcat
     1921 gacagcttga cgaaagaccg cgccggcgca atggcaaatg ccgtccgcga acgggccgcc
     1981 gatattcacg aagccgatgc cctcaaggga cgcggcggcg gaggacacgc caaaaccgcc
     2041 cggaaaaagc cgggcgcaaa tcagggcggc ggcaaagggg gcaaatcccg ttcgggcggt
     2101 ccgggcgcgg ccaaagaccc gatgcaggct tgggaagggg aaatcaaagc ccgaaaactc
     2161 gcacaccgcg aaatgcagcg cgaaacgctc acgcaccaag aatgggattt ggcgcgtgag
     2221 gcggaatatt ggcgggcgaa gctggcaacg gtggacgcca acggaaaaac aggcgtaaaa
     2281 atccgcgaaa aaatcctgac gcttgaagac caattatcga agcagtcaac cgaagccaaa
     2341 atgaatcagg cggccgaatg ggagaaattg gacaagcaca agctggagat ggagaaagac
     2401 gcggcagacc aagccctggc agacggacgt atttcgcaac tcgaacgcct ggacttggaa
     2461 atcgagtttg aaaaccgccg ttaccaaatc gcctatgacg cattgcagga acgcatcgca
     2521 cttgccgaac aagacccggc atacagtcag gcggcaatcg gcaagctcaa ggcccaaatg
     2581 ggcgaattgg ggcgggggca cgaacggacg caggcgaaga acgagggcaa acgcgaaaac
     2641 caacgccgca aagacgcgcc caacgtcatg gaaatgctgc aagacggcgg caggaacgtt
     2701 tggcaggaag cacagcagca gatggggcag gcgttcaccg ccatgctggc gagggcgcag
     2761 aatttcaggc aggcgatgaa cggctttttc aagagtatgg ggcaaacctt tattcaggaa
     2821 atggttacga aacctttgac gggcttgatg cggcgcatgg ttgaggaatc ggccatttac
     2881 aagatgattt tcggaactaa ggaaacgctg gagacagcgg cggcagccaa gacggcggca
     2941 actaaggcaa ccgagacaac ggcggttgtc agtagtaatg ctgttcaagc ggcttcaggc
     3001 gcggcggctt cccaggcgca tatcccatat gtcggcccga ttcttgccgt tgcggcaatg
     3061 gcggcaatga tgtcggcagt gatggggttg atgggcggcg gcggcggttc tcaaacgacc
     3121 acaaccacga cgcgcattcc ctcggcggca ggcggctggg acatcccggc aggcatcaac
     3181 ccgctgaccc aactgcacga aaacgagatg gtcctgcctg ccgaacacgc ccaaaccatc
     3241 cgtgaaatgg cggatcagca gggaggcggc agcaccgtca tcatcaattc gacaggaggc
     3301 gatttcatcc ataaaaatga cttggcgaag ctcttgaagc agatgaaacg ggactttaaa
     3361 tttgtctgag aaaatgccgt ccgaaaggtt cggacggcct atgttgcggc gtacgtcaag
     3421 ctattaagct atttggtttt cagaggcttt ttcttccatt tccgggctac ggcttcaaat
     3481 tcttgaaagg ccgtcggcgc gctcctgata cggcgtattt caaaaatgaa gctgcttagg
     3541 ttgtcccagt cgcgtaacag catggagtat tcgaaatctt tatacatttt ctcgtgcagc
     3601 gccccgccca atacgcccgc acagacaaat tcgcgctggt ttaaaactgt cagtatggct
     3661 tcacggtctt tcttcttgtc cggggtatct gatgtatagg tggcgagtat gcatccgtct
     3721 gtttttgcca gcccgtttac tatggttatg gcttcttgaa gggcggcatt gttacgttcg
     3781 gccatgatca tgtcgatggt ggcgcgcttt ttggcgtttt ccgtattcct catgatgccg
     3841 taagcggcaa caaaaacgcc gataacagtc aatatcggcg ttgctatctg tattaggttg
     3901 tcagtcattg ccgtcccagc cgtccgaaag gcggaaatca cggctttccg aataaatatg
     3961 ccccattgtc atccccttaa gctgatgttg agaataaatc cttgcgtcgg aatatacggc
     4021 attgcctgac tattttcaag attccgcacc ccgcggaccg gactatacaa aaaaaccgcc
     4081 ttcgtgtaaa gcggttgaag agagcctttc aataaaaatg ccgtccgaag ggctttcgga
     4141 cggcattttt tatagattct tttttaatga acctttgaaa ccgaagataa ttaattttcc
     4201 tctttatttt caatacgcag tgtgccctat gtttttaaat aaaaatattt taatccaaca
     4261 tttttgcaat atccgccatg tcggggcggt agtaaacatt gagtaaaatc cttaaatccc
     4321 gatgcccgct gatttttgcc aagacctcca caggcacttt ttttgatagg cgcgtgaggg
     4381 cttcgcggcg ggtgtcgtga aaatgcaggc cttgtatgcc gcaattatcc cgcgctcgcc
     4441 tgaataatac atcaaggctt ttggcatcca ggctaaacac ccatgtgtca tcaattccgc
     4501 gcagttgctc tattagttcg gcggctcgca aggataatgg cacgtcacgg ctgtcgccgt
     4561 ttttagttat tgggaggtgg gcaatacgac gccgcatatt cacatctgcc cattttaggc
     4621 cgcagatttc gcctgcccgc atggcggttt cgatggcaaa taggacggca acggcaaccc
     4681 gttgcaccgc cattttcggt ttctcattag gccggtatag gagggcggcg caaatatcgg
     4741 caatttcctg ctcggttggc cgtcttgtcc ttgcccggct ttttttcggc ttgctgattt
     4801 tgcgtacagg gttttcgcga agaagtcccc actctttcat ggcgtgttcg cagacggcgg
     4861 ataaagtggt taattcgcgt ccgacgctgg tgggggatac ctcctgcaac cgttgatctc
     4921 gccaatcggc gaagtcttga gggcgcaggt cggcaagccg cacctttgcc aaaggtgttt
     4981 ttagtgcgcg tccaatgcgg tatgattctt cccgtgcgcc ccgctttgat ggtgtaacct
     5041 cggataaata ccgccggata aggtcggcaa agatagtatt tcgcggggca ttgccttgga
     5101 tgccgtccat aatttgcgac tctattcgcg ccgcccacgc cttcgcgtct gatgcccgct
     5161 caaatgtggc ggaacgggaa acgcctttca tacggatttg tacgcgccat ttgccgttac
     5221 gttgagtgat ggttgccata tttattcggg tgcaattcgg gtgcaaatat aatagagcaa
     5281 aacagatata aagcggggca aaatagggct atattaaatt taatgctttg atatgtaggg
     5341 ataaatagat ttaaaaagac gaaagaagtg cggacgtaga ataagcgtag atgcgctccg
     5401 tccgcaccaa taaaaatata taaatcatat tgttaatgat tattgggaca attcggtggc
     5461 acaaaatata agctgtctgc ttttcagcgg gcggcttttt atctgcctat gtgtttcaac
     5521 acacaaaacg gcacataaag caccgccatt tgttccgcac cgaatcggaa gcgggtgctt
     5581 ccgcccgcaa tcggattcgc cgcccctaat gtcggggttt taaccggaaa gaagaaaata
     5641 ttgattttga tgatggcatc aaaaaaaccc ttccgcccgt ctgccaaaag gaatggttaa
     5701 gaaaagcaat gcgtcttttg atggaacgcg atatataaga aattgagggg aagggcaggc
     5761 gggtttctgc ccgttaatcc tgcggacggc cccgccaaac ccgacacgcg cgggcatatg
     5821 gcggattgtt tacaatattg tctaaaaatc tgaacctgca aacgcgaaat aaggtagaat
     5881 acgccccgtt attttaccgt ccaatcccca tttaccaagg aaaaacgatg agtacttcat
     5941 tgagttaccg cgatgcgggc gtcggtatcg acgcaggcga ccaactggtc gaaaaaatca
     6001 aaccgtttgc caaacgcacc atgcgtccgg aagtattggg ggatttgggc ggttttggcg
     6061 cattggtcga aatcggcaaa aaatatcaaa accccgtatt ggtcagcggt acggacggcg
     6121 tgggtaccaa gctcaaactt gccttcgatt gggacaaaca cgatacggtg ggcatcgacc
     6181 ttgttgcaat gagtgtcaac gatattttgg ttcaaggagc tgaaccattg tttttcttgg
     6241 attattttgc ctgcggtaaa ttggatgttc cgcgtgcgac cgatgtcatc aaaggcattg
     6301 cccaaggttg cgaagaatcc ggctgcgccc tgattggcgg ggaaactgcc gaaatgccgg
     6361 gtatgtatcc cgttggtgaa tacgatttgg cgggttttgc cgtcggcgtg gtggaaaaag
     6421 agaatgtcat taccggccgc agcatcggcg cgggcgatgt ggtattgggt ttggcttcca
     6481 acggcgcaca ttcaaacggc tattccctta tccgtaaaat catcgaacgc gacaatcccg
     6541 atctggatgc cgagtttgat aatggcaaaa ctttacgtga agccgttatt gcgccgacac
     6601 gtctgtatgt gaaaccgatt cttgccgctt tggaaaaatt taccattaaa ggtatggcgc
     6661 acattaccgg cggcggcatt accgaaaacg tgccgcgcgt gttgcctaaa aacacggttg
     6721 cccaaatcga tgccgaatcg tgggaattgc ccaagctctt ccaatggctt caaaaggcgg
     6781 gcaatgtgga aacccaagaa atgtaccgga cctttaactg cggcatcggt atggtcgtca
     6841 tcgttgctgc cgaagatgcc gatgcggttc ggtcattcct gagcgggcag ggcgagacag
     6901 tgtaccgttt gggttgcatc cgagagcgtc aagggaacga gcatcaaacc caagttgcct
     6961 gacttccaag catcaaatgc cgtctgaatt tgtttcagac ggcatttccg catcttccaa
     7021 acgcgtatcc gcaacacagt tctgcaaact gatattttgc ccggtatggc ggcttaaact
     7081 tcttcatcat atttcgataa tgctataaaa tgatataatc ataaaatatt gtttattcct
     7141 acatcgtatt catcctattt tccgaaatgt gagcgtttta tgaagcaatc cgtccataat
     7201 aaaacaattt taagtgtttt gatttcat
//
LOCUS       DBJGGI010000082         7175 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.082, whole genome shotgun sequence.
ACCESSION   DBJGGI010000082 DBJGGI010000000
VERSION     DBJGGI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7175
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.082"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..719
                     /gene="cysE"
                     /locus_tag="ACSIGJ_001854"
     CDS             <1..719
                     /gene="cysE"
                     /locus_tag="ACSIGJ_001854"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HHQ1330665.1"
                     /translation="LHQTVLRHESLGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSD
                     TQIGKCVEADLKAIYERDPACDEYSLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFL
                     QNRMSEVFGVDIHPAARFGYGLMLDHATGFVAGETAVLGNNISILHGVTLGGSGKEGG
                     DRHPKIGDGVMIGANASILGNIRIGSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSL
                     KTPSADMDQNIQFTEIDFMI"
     gene            complement(1076..2587)
                     /gene="ubiB"
                     /locus_tag="ACSIGJ_001855"
     CDS             complement(1076..2587)
                     /gene="ubiB"
                     /locus_tag="ACSIGJ_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HHQ1330666.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQPIEKLYAEFETDPVASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGATWVERLFSDGRRLKPREVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDFDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKINALVDEARRQEMRDAYIHLVKVQQRQSLWLAAIAFVLLLILLLK"
     gene            complement(2622..3086)
                     /locus_tag="ACSIGJ_001856"
     CDS             complement(2622..3086)
                     /locus_tag="ACSIGJ_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HHQ1330667.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLKENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     ADGEMPEYLELHGGREDDISAPSQPEPPPRNIKPLVFGLLIAFLSYLLGKIF"
     gene            complement(3083..3229)
                     /locus_tag="ACSIGJ_001857"
     CDS             complement(3083..3229)
                     /locus_tag="ACSIGJ_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330668.1"
                     /translation="MPSERSDGIFTFGKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVLL"
     gene            complement(3246..3584)
                     /gene="erpA"
                     /locus_tag="ACSIGJ_001858"
     CDS             complement(3246..3584)
                     /gene="erpA"
                     /locus_tag="ACSIGJ_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HHQ1330669.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(3716..4402)
                     /locus_tag="ACSIGJ_001859"
     CDS             complement(3716..4402)
                     /locus_tag="ACSIGJ_001859"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HHQ1330670.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVGHEEPVFTMAFRRHWIESYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HSENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            4589..4861
                     /locus_tag="ACSIGJ_001860"
     CDS             4589..4861
                     /locus_tag="ACSIGJ_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330671.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            5069..6997
                     /gene="dnaK"
                     /locus_tag="ACSIGJ_001861"
     CDS             5069..6997
                     /gene="dnaK"
                     /locus_tag="ACSIGJ_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HHQ1330672.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSIIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKADNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKIAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKTAIDAKAEALGTASQKLGEMVYAQAQAEAQAGEGAQANASAK
                     KDDDVVDADFEEVKDDKK"
BASE COUNT         1986 a         1937 c         1628 g         1624 t
ORIGIN      
        1 ttttgcacca aaccgtgttg cgccacgagt ccctcggctc cgtccttgcc taccaccttt
       61 ccagcaaact cggcagcccg attatggacg tgcgcgcgct gtttgaaatc taccagcagg
      121 cgttgggcag cgacacccaa atcggcaaat gcgtcgaagc agacttaaaa gccatctacg
      181 aacgcgatcc cgcctgcgac gaatattcgc tgccgctttt atatttcaaa ggcttccacg
      241 cgattcaggc acaccgcatc aaccatcggc tgtatctcga cgggcgcaaa acgctggcgt
      301 atttcctgca aaaccgtatg tccgaagtat tcggcgtgga catccatccc gccgcccgtt
      361 tcggatacgg gctgatgctc gaccacgcca ccggctttgt tgccggcgaa accgccgtgt
      421 tgggcaacaa tatttcgatt ttgcacggcg taacgctcgg cggttcgggc aaagaaggcg
      481 gcgaccgcca ccctaaaatc ggcgacggcg tgatgatcgg cgcaaacgcc tcgatattgg
      541 gcaatatccg catcggcagc aatgccaaaa tcggggcggg cagcgtcgtg gtttcagacg
      601 tgccgccgtc catcacggtt gtcggcgtac ccgccaaacc cgtggcgcga tcgctcaaaa
      661 caccgtcggc ggatatggat caaaatatcc agtttaccga aatcgacttt atgatttgaa
      721 tatccgcacc aatgccgtct gaaacgccaa acgggcttca gacgacattg cccgcttcaa
      781 gcctgcgcct tgatatgccg ccgatgtcgg cacttgccag tcggacaaaa tgccttttat
      841 tcgatacacc cgattcccac gcaaccggaa atatagtgga ttaacaaaaa tcaggacaag
      901 gcgacgaagc cgcagacagt acggatagta cggaatcgat tcacttggtg cttcagcacc
      961 ttagagaatc gttctctttg agctaaggcg aggcaacgcc gtactggttt ttgttaatcc
     1021 actataaccg atttccttat aaacgggcgg agtcgggcga tgattcggac aaagcctatt
     1081 tcaaaagcaa aatcagcagc aaaacaaacg cgattgccgc cagccacagg ctttgccgct
     1141 gctgcacttt gaccaaatga atataggcat cgcgcatttc ctgccggcgc gcctcatcga
     1201 ccaaggcatt gattttgcgc ggcaatgaag ggatgatttg cgcccagtcg ggggcttcgt
     1261 ttttgaggtt gcgccaaagg gctttagggc cgacctgccc gttcatccat ttcaccaaaa
     1321 acggtttggc ggttttccac aagtccaaat cgggatcgag ttggcgaccc agaccttcaa
     1381 tattgagcag cgttttttgc agcagcacaa gctgcggttg gatttcgaca ttgaagcggc
     1441 ggctgacttc aaacaggcgc atcagcacca agccgaagga aatctgcgaa atcggtttgt
     1501 tgaacacagg ctcgcacacg gcgcggacgg cggcttccaa ttcttccgca cgcgtgtcgg
     1561 caggcaccca gcccgattcg atgtgggcgg tggcgacgcg ccggtaatcg cggttgaaga
     1621 aggcgaggaa gttgatggcg agataacgtt tgtcgaaatc ggtcagcgtg ccgacgatgc
     1681 caaaatcgag ggcgatgtag cggttgtcgg cggcgaccaa aatattgccg gggtgcatat
     1741 ccgcgtggaa aaagccgtcg cggaagactt gcgtgaagaa gatttccacg ccgtaatcgg
     1801 cgagtttgtg caaatcgatg ccgtctgctt tgagtttggc gatgtcggaa accggcgtgc
     1861 catccatcca ttcgattgtc agcacgtcgc tggtgcagta gtcgtaaaac accttgggca
     1921 caatcagcat attgctgttt tggaaattgc gtccgagctg tccggcattg gcggcttcgc
     1981 gcatcaaatc caactcgtcg tgcaggtatt tgtcgaactc cgccaccact tcgcgcggct
     2041 tcagacgcct gccgtctgaa aacaagcgtt cgacccaggt cgcgccgaag cgcatcagcg
     2101 acaagtcctg ttcgataagg ggcaaaaggt tggggcgcaa aactttgacc gccactcgtt
     2161 cgcccgaatg caggcgggct ttgtgtactt gggcgatgga cgcgctggcg acgggatcgg
     2221 tttcaaattc cgcatacagc ttttcgatgg gctgacccaa cgatttttcg atttgttcac
     2281 gcgaaagccg cgcgtcaaaa ggcggcactt tgtcttgcag ctttgccagt tcgacggcgt
     2341 aatcgtgcgg aatcaaatcg gggcgtgtgg acaaaacctg cccgaacttg atgaaaatcg
     2401 gccccaggct ttccaaggca aggcgcagac ggacggcagg cggttcgttt ttcaatttgg
     2461 acgactgcgg catcattttc agcagagcgc atatccaacc gctacccatc agcgaagcgc
     2521 acagacctgc cagccgatag cggtaaaaag tcccgacaat gaccgtcagg cgtttcaacc
     2581 atttcatata taaaatcccg aatacagcat ttatcggaca atcaaaaaat cttaccgagc
     2641 agatagctca aaaaggcaat cagcagcccg aaaaccagcg gtttgatatt gcggggcggc
     2701 ggttcgggtt gcgaaggtgc ggaaatatca tcttcccgcc cgccatgaag ttcaagatat
     2761 tcgggcattt ccccatccgc tccttctcct tcgccctccc cttcctccgg cggcacgacc
     2821 gtttccaact ccatatatct ccgggtaata atatccaaac ccaaatagct gaaataatcg
     2881 tgcaactctt cccgtttcgc ccaagtgcat ttttcctcaa ccagcacttg cggtttggaa
     2941 tcgagcgatt cgatcaatcc ttccgccttt tcctcgccga gattctcctt aaggcactcc
     3001 cggataagcc cgcgatccac ttggggcggg taggaaacat atacatcgta caaatcgttt
     3061 cggtaatctt tactcatctc aatcataaga gaacaaatac gcctgatacc gttccgcctg
     3121 ttcctttcgg gaaacccgat tcatcttcaa acaatcggat tatatgtgaa ttcatcccga
     3181 taatatattt tccaaaagta aaaatgccgt ctgaacgttc agacggcatc acacaaacca
     3241 agcggtcata cggaaaacga cgatccgcaa ccgcaggttg tttccgcatt cggattgcgg
     3301 atgacgaatt gcgaaccctg caaactttcc gtatagtcga tttccgcacc gactagatat
     3361 tgatagctca tcggatcgac caaaaagacc aaaccgtttt tctcaatttc aaaatcgtcg
     3421 tcgtttttga tttcgtcaaa agtaaatccg tactggaaac ccgagcagcc gccgccgttg
     3481 acaaaaaccc gcaatttcaa atcgggattg ttttcttcgg caatcaaatc ggcaactttg
     3541 gcacagcagc tgtcagtaaa aataataggg ctttcatccg acatggtgtt attccttaat
     3601 ataatatttt ctgcattctc tctcaaaccc ccttcaaagg caagcaggct tcgcctttga
     3661 aaaaggaatt gttccaatag ataagtttga ccgcctgcaa tttcaagccc caaactcaaa
     3721 tcgtcctgcc gaaccactcg acacgcccga taatctccac atcatcggtc aaatcgttca
     3781 aattgatttc aaaagcagga taagcctcgt ttgcagaaat cacattgata atcccgcccg
     3841 gtacaatctg taaacgttta accagcagat tttcattaat ccgcaacaca tacagaccgt
     3901 ccctcggcgt gttctcgcta tgattgacca aaatcgaatc gccgtcattc aaaaccccct
     3961 ccatcgaatc ccccttaacg gaaattacag acaggttttt cgtatcgcgg gtaacgtagc
     4021 tctcgatcca atgccgtctg aacgccattg taaataccgg ttcctcatga ccgacaaact
     4081 gcccgtatcc cgcagccgcc cgaatatcat atctcggcac aaagacaaac tcgtccgtat
     4141 caacttcatt gcccaaggta tcgtaagcaa gggatttttt tggggcttca tccggaaacg
     4201 gattaccctc cccggtcagc agccaatcga tactacaccc cttcaactgc ttgatttttt
     4261 tcaatgtttc agacttcggc agaccgcctt cattccatat cctgctgaag cccgcaatcg
     4321 tcatttcaat atcggatgcg atttttgcct gcttcgcttc gcttttccat aaaaaaacca
     4381 gtctgtcttt aaaggtttcc ataaatatcc ttccgctccc cttcattcaa tcatctaaag
     4441 taaacatgaa atatatttta ccttagctat cccaaaaacg gaacaactat gataaactaa
     4501 atttaaatta tgatttttct tactcaatac ataattttta agatttttcc ataggtttaa
     4561 tcctgaattg acagaaaaag gatagaaaat gaagaaaacc ggcaaatatc ttatctatgc
     4621 tgcggcattt accgcattct gctttgcctt ccaagaaaac cgttctgaag ccaaacagcc
     4681 tgacatcact ttatccgcat ccctgtgcga acaattcaac atactgaacg ccaaagatat
     4741 ggatgcagaa caagtctccc tttccaaaga atgcgacatc atcgagtctt cacacgactg
     4801 ggaaaaagag tacggcaact tgaacgaaca ggaaatgctc gccggcattg tctatgaata
     4861 aacctgcctg ccatttgaaa cattatgctt gaatgcattg gagccaaatg tattaaatca
     4921 aatataaaac caatatattc ataaagttat atacttatag ccatgctgta gcttgaaaca
     4981 gcccgccgcc cgccctattt acagcccatc gggacaaaat gttccaacat atttctcaaa
     5041 ataagcaaaa tcaaatagga gtatccacat ggcaaaagta atcggtatcg acttaggtac
     5101 aaccaactct tgtttggcca tttccgaaaa cggtcaaacc aaagtcatcg aaaacgcaga
     5161 aggcgcacgc accacgccgt ccattatcgc ttatttggac ggcggcgaaa tcctcgtcgg
     5221 cgcgcctgcc aaacgccaag cggtaaccaa cgccaaaaac actatttacg ccgccaaacg
     5281 tttgatcggt cacaaatttg aagacaaaga agtccaacgc gacatcgaat ctatgccttt
     5341 cgaaatcatc aaagccgaca acggcgacgc atgggtaaaa gcacaaggca aagagctgtc
     5401 tcctcctcaa atttccgcag aagtcctgcg taaaatgaaa gaagccgccg aagcttactt
     5461 gggcgaaaaa gtaaccgaag ccgtgattac cgtccctgcc tacttcaacg acagccaacg
     5521 tcaagccacc aaagacgcag gccgtatcgc cggtttggac gtaaaacgca tcatcaacga
     5581 gccgaccgca gccgctttgg cattcggtat ggacaaaggc gacaacaaag accgcaaaat
     5641 agccgtatat gacttgggcg gcggtacctt cgatatttcc atcatcgaaa tcgccaacct
     5701 cgacggcgac aaacaatttg aagtattggc taccaacggc gatactttct tgggcggtga
     5761 agacttcgac caacgcttga ttgactacat cattgacgag tttaaaaaag aacaaggcat
     5821 tgatttgaaa caagacgtaa tggctctgca acgtctgaaa gaagctgccg aaaaagccaa
     5881 aatcgaattg tccagcggcc agcaaaccga aatcaacctg ccgtacatta ccatggacgc
     5941 aaccggcccg aaacacttgg caatgaaaat tacccgcgcc aaattcgaga gcctggttga
     6001 agacctgatt gcccgctcta tcgagccttg ccgcaccgca ttgaaagatg ccggcttgag
     6061 caccggcgac atcgacgacg tgattttggt cggcggtcag tcccgtatgc cgaaagtaca
     6121 agaagccgtt aaagacttct tcggcaaaga accgcgcaaa gacgtgaacc ctgacgaagc
     6181 cgttgccgta ggtgcagcga ttcaaggcga agtattgagc ggcggccgca gcgacgtatt
     6241 gctgctggac gtaacccctc tgtccttggg tatcgaaacc atgggtggtg tgatgaccaa
     6301 gctgattcaa aagaacacta ctattccgac taaagcgtct caagtgttct ctactgccga
     6361 agacaaccaa agcgcagtaa ccatccacgt actgcaaggc gaacgcgaac gcgcttctgc
     6421 caacaaatct ttaggtcaat tcaacttggg cgacatcgca cccgcaccac gcggtatgcc
     6481 gcaaattgaa gttactttcg acatcgacgc caatggtatc ttgcacgttt ccgccaaaga
     6541 caaaggcact ggcaaagcgg ccaacattac catccaaggt tcttcaggtt tgagcgaaga
     6601 agaaatcgaa cgcatggtga aagatgccga agccaatgcc gaggaagata aaaagctgac
     6661 tgaattggtc gcttcccgca accaagccga agccctgatt cactccgtga aaaaatcttt
     6721 ggcggactac ggcgacaaac tcgacgctgc cgagaaagaa aaaatcgaag ccgcgctgaa
     6781 agaagccgaa gaagccgtga aaggcgacga caaaaccgcc atcgatgcca aagccgaagc
     6841 actgggtaca gccagccaaa aactgggcga aatggtttac gcgcaagcgc aagccgaagc
     6901 ccaagccggc gagggcgcac aagccaatgc ttctgcaaag aaagacgatg atgtcgtaga
     6961 tgccgacttt gaagaagtaa aagacgacaa aaaataattg atgccgtctg aaaaaaacgc
     7021 gaaccattcg gttcgcgttt ttttcaattg agataaaaga caatagcatg acagagattc
     7081 caaattcatc taccgtgata ttcccaagcc caagttccaa ctgttgcatc ggttatctgg
     7141 aaatttttca tatcattttc ttatttactt aaatt
//
LOCUS       DBJGGI010000083         7097 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.083, whole genome shotgun sequence.
ACCESSION   DBJGGI010000083 DBJGGI010000000
VERSION     DBJGGI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.083"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(958..1641)
                     /locus_tag="ACSIGJ_001862"
     CDS             complement(958..1641)
                     /locus_tag="ACSIGJ_001862"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HHQ1330673.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     KYGDEQVRIWRRSYDTLPPLLDKDDAFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            1790..4093
                     /gene="parC"
                     /locus_tag="ACSIGJ_001863"
     CDS             1790..4093
                     /gene="parC"
                     /locus_tag="ACSIGJ_001863"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HHQ1330674.1"
                     /translation="MNTQPHASHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFMPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDRLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRVVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAVFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQFGDARRTL
                     VEEAGRAVLTQTAADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSSGRTYSIDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRLPV
                     ALIEAKRGKKGRLLPISGSLKQLSSPK"
     gene            4113..5573
                     /locus_tag="ACSIGJ_001864"
                     /pseudo
     CDS             4113..5573
                     /locus_tag="ACSIGJ_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237220.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            5661..5837
                     /locus_tag="ACSIGJ_001865"
                     /pseudo
     CDS             5661..5837
                     /locus_tag="ACSIGJ_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003743698.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            5937..6689
                     /locus_tag="ACSIGJ_001866"
     CDS             5937..6689
                     /locus_tag="ACSIGJ_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHQ1330675.1"
                     /translation="MTDILIDDTATEAVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSNVIVDFTSGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLTVTAFEQHPAVACLLSDGIRRALLNPETQDTAARINLHFGNAAEQMPALVKTQG
                     KPDIVYLDPMYPERRKSAAVKKEMAYFHRLVGEAQDEVILLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGADKG"
     gene            complement(6867..>7097)
                     /locus_tag="ACSIGJ_001867"
     CDS             complement(6867..>7097)
                     /locus_tag="ACSIGJ_001867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330676.1"
                     /translation="SFPQKRESRSLGSGNLQKSSEKSEVLDSRFHGNDGISDGILPPR
                     HSRAGGNLVRSVSVIYDKFLLPFISGFPLSRE"
BASE COUNT         1708 a         2102 c         1747 g         1540 t
ORIGIN      
        1 ctactatttt agtagtttta ccattagaaa aaggatatga atggaattgc cttgaggtag
       61 aagtactatc cgctgctcaa tctctagcaa atatctattg caacaaggtt cgcctcatag
      121 atggtcatgt ttgggaggaa taaatgaaaa agcgtagttg gtaattttta gttgcgcagg
      181 gtgggcttca gcccaccgtt ttttcttcat tttgggttgt tgattggtgg gctaaagccc
      241 accctacgat tactaaaatc ccgtcattcc cgcgtagagc gcatcatata aggcgggaat
      301 ccagaccttt cggtgcgaaa acttatcgga taaaacggtt tcccctgcat cctggattcc
      361 cactttcgtg ggaatgacgg gatataggtt tccctacgga cgtgttcaga ttcctgcctt
      421 cgcgggaata acggcggagc agtttctgct tttccaataa atgaccgtaa cttaaaatcc
      481 cgtcatcccc gcgaaagcaa aaatcctgcc tgtcggattt cggttttttc gggaaactga
      541 tgaatcgtca ttcccgcgca ggcgggaatc cagaacgcgg gacggcggca atattcaaag
      601 gttgtctgaa aattcagagg ttccagattc ccgccttcgc gggaatgaca gcggagcagt
      661 ttctgctttt ccgataaatg accgcaacct aaaacccatc cttcacacga aaacagaaaa
      721 acaaaaacct aaaatcccgt cattcccgcg ataacagttg cgtaattgcg tagggtgggc
      781 ttcagcccac cgtttcttca tttttgcttg tcaattagtg gactgaagcc caccctgcgg
      841 ttactgtttc ttctttttcg attattaatt ggcaggctga agccctccct acgtttaccg
      901 tcaaaaatgc cgtctgaaat gctcaagccc tcagacggca ttttttattt cgcaccctta
      961 caggtaaaat ttctcgatga ctttcaggtt gtcgtctaat ttgtacacca gcggctggcc
     1021 ggtcgggatt tccaagccca tgatgtcttc gtcggaaatg ccctcgatgt gttttgccag
     1081 cgcgcgcagg gagttgccgt gcgccgctac caagacgcgt ttgccgctca aaatcgcggg
     1141 ggcgatttgg tcttcccaaa acggtaatac gcgctccagc gttactttca ggttttcgcc
     1201 gtcgggtacg acatcggcag gcagatgggc atagcgacgg tctttgtgtg cggaaaacgc
     1261 gtcgtctttg tccaaaagcg gcggcagggt gtcgtagctg cgccgccaga tgcggacttg
     1321 ctcgtcgccg tatttttcgg cggtttgttt tttgtccaaa ccttgcagtc ggccgtagtg
     1381 gcgttcgttc agcctccatg ttttgatttg cggtacgaac agttggtcgg attcttccaa
     1441 aacgatgttg caggtcttaa tcgcgcgggt caggacggat gtgaaggcga tgtcgaactc
     1501 atagccgttt tctttcagtt ttttaccggc ggcggcggcc tcggcaagcc cctgctcgct
     1561 cagcttcacg tcgcgccagc ctgtaaacag gtttttcgcg ttccattcgc tttgtccgtg
     1621 gcggataaat accaattcca tatcgtctcc aatgtgtgaa agtgggaaag ctttatttat
     1681 aacatatttt cacatttccc gtatttgatt cagattcagg cacggcgccc actatggttt
     1741 gccattttga tttacaataa tgtcctttgc tttacattcc gcatacacaa tgaatacgca
     1801 accgcacgct tcccataccg attccaacac gctgatgctc ggccgatacg ccgaacgcgc
     1861 ctatctcgaa tacgccatga gcgtggtcaa aggccgcgcg ctgcctgaag tttcagacgg
     1921 ccaaaagccc gtgcagcggc gcattttgtt tgccatgcgc gatatgggtt tgacggcggg
     1981 ggcgaagccg gtgaaatcgg cgcgcgtggt cggcgagatt ttgggtaaat accatccgca
     2041 cggcgacagt tccgcctatg aggcgatggt gcgcatggct caggatttta ccttgcgcta
     2101 ccccttaatc gacggcatcg gcaacttcgg ttcgcgcgac ggcgacgggg cggcggcgat
     2161 gcgttacacc gaagcgcggc tgacgccgat tgcggaattg ctgttgtccg aaatcaatca
     2221 ggggacggtg gattttatgc cgaactacga cggcgcgttt gacgagccgc tgcaccttcc
     2281 cgcccgcttg cctatggtgt tgctcaacgg cgcgtcgggc atcgcggtgg gtatggcgac
     2341 cgagattccg tcgcacaatt tgaacgaagt cacgcaggcg gcgattgcac tgttgaagaa
     2401 accgacgctg gaaaccgccg acctgatgca atatattcct gctcccgatt ttgccggcgg
     2461 cggtcaaatc atcacgccgg cggacgaatt gcgccgtatt tacgaaaccg gcaagggcag
     2521 cgtgcgcgtg cgtgcgcgtt atgaaatcga gaaattggcg cgcggacagt ggcgcgtcat
     2581 cgtaaccgaa ctgccgccga acgccaattc cgccaaaatc cttgccgaaa tcgaagagca
     2641 aaccaacccg aaaccgaaag cgggtaaaaa gcagctcaac caagaccggc tcaataccaa
     2701 aaagctgatg ctggatttaa tcgaccgcgt gcgcgacgag tccgacggcg aacatcccgt
     2761 gcgccttgta tttgaaccca aatccagccg catcgatacc gataccttca tcaacacgct
     2821 gatggcgcaa acttcgctgg aaggcaatgt gtccatgaac ttggtgatga tgggtttgga
     2881 caaccgcccc gcgcagaaaa acctgaaaac gattttgcag gaatggctgg atttccgcgt
     2941 cgttaccgta acacgccgtc tgaaattccg tttaaaccaa gtggaaaaac ggctgcacat
     3001 cctcgaaggc cgtctgaaag tctttctgca catcgacgaa gtgattaaag tcatccgcga
     3061 atcggacgac ccgaaagccg atttgatggc ggtgttcggg ctgaccgaaa tccaagccga
     3121 agacattttg gaaatccgcc tgcgtcagct ggcgcgtttg gaaggtttca aactcgaaaa
     3181 agaattgaac gaattgcgcg aagaacaagg ccgtctgaac atccttttgg gcgacgaaaa
     3241 cgaaaaacgc aagctgatta tcaaagagat gcaggcggac atgaagcagt tcggcgacgc
     3301 gcgccgcacg ctggtggaag aggccggacg cgccgtgctg acacaaaccg ccgccgacga
     3361 acccatcacg ctgattttgt cggaaaaagg ctggatacgc agccgtgccg gacataatct
     3421 cgatttgagc caaaccgcgt tcaaagaagg cgaccgcctc aaacaaaccc ttgaaggccg
     3481 cactgtttta cccgtcgtca tcctcgattc atcgggcaga acctactcga tcgatgccgc
     3541 cgaaatcccc ggcggacgcg gcgacggcgt accggtttcc tccttaatcg agttgcaaaa
     3601 cggcgcgaaa cccgtcgcga tgttgacagg attgccggaa caacattatt tattatcaag
     3661 cagcggcggc tacggcttta tcgccaagct gggcgatatg gtcggacgcg tgaaagcggg
     3721 caaagtggtg atgaccgcag acagcggcga aaccgtcctg ccgccggttg ccgtctatgc
     3781 ctcctcgttc atcaaccccg actgcaaaat cattgcagcc accagtcaaa accgcgccct
     3841 cgccttcccc atcggcgaat tgaaaattat ggcgaaaggc aaaggactgc aaatcatcgg
     3901 attaaacgcc ggcgaatcga tgacgcatac cgccgtttct tccgagccgg aaatcctgat
     3961 tgaaagcgaa ggcaggcgcg gcgcggcgca caaagaccgc ctccccgtcg ccctgattga
     4021 ggcaaaacgc ggcaaaaaag gcagactgtt gcccatatcg ggcagcctga aacagctttc
     4081 ttcccccaaa taaacccggt tccgcacata ttatggtgat ttccaacccc cgcgaacttg
     4141 aaaaactcaa agaccggatt cccaatctga tcaacatcat ccgcgtcgcc atcgttttcc
     4201 cgctgatgat tatgcacatc ctcgggctgg aaaccggcag ccgtgcgaac ctgcacgcct
     4261 catggacggc gtgggcgttt tacttgtggc ttgccattgc ctgctggctg attttctttt
     4321 ccaccctcaa cccgcaatgg caatggcaat ggcaggcttt gagaataccg agtttcagcg
     4381 ccgtggccga catcacgatg atcggcgtgc tgacctacct gttcggcggc atcgattcgg
     4441 ggttcggcat cctgatcctg cccttcgtgg gcagctcctg cctactcagt tacgggcgtt
     4501 atcccctgct ctatgccagc tatgcatcca tcctgctgat attcaacgcc cttgcagaca
     4561 gcaatatcaa tatgtatccg ctgattctgg atgcaaaaac cgtcaccaat accttcgtcg
     4621 tcgtagccgg ctcctatttc gtcgctatga ttgcctcgct gtcggtcaga tacatcgacc
     4681 gtgccggcaa acttgcccat gaaaaccacg tcgcctaccg ccgtatcagg ggcttgaacc
     4741 aaatcgtgct caaccgcgtt caggaagcgg tcgtcgtcat caacgtcgag catcagacca
     4801 tactgttcaa taaaaaggca aaagatctgc tccccatgct tgaaatcgga cagcatacct
     4861 ccctgttcga ccctgtcgcc atcttatggg ataaaacatc ctcacgcacc ttcgaacact
     4921 atatcgacac gcccgaactg accgcccgca tccgcgccgt gccgatgaac aaaaagcaga
     4981 acaagctgct catcctctat atccgcccgc aaagcgaaat tcaggcggaa gccctgtccg
     5041 tcaaacttgc cgcgctcgga caactgaccg ccaaccttgc ccacgaaatc cgcaacccga
     5101 tgtccgccat ccgccacgcc gacggcggca ctttattttt ggacgaagtg gccgacctgc
     5161 ccctttccat gcaggtcaaa ctcttgcgcg cgattcaaga aaaagccgtg cgccgtatcg
     5221 gcgacgcgac cgagcagccc gtcgatgtcc gcatcgtctg cgccacccac aaaaacctcg
     5281 aagcccttgt cgaaagcggc gcattccgcc aagacctgta ttaccgtctc aatgtcgtca
     5341 gcctcaatat gccgtccctg cgtgaaatgc gcgaaaattt gaagctgccc accccctacc
     5401 tcctgtacaa acacagccac aacaaccggc cctacacact ctcccccgcc gcgcaacaga
     5461 tgctcctgaa ttacagttat ccgggcaatt tccgcgaact cgaaaacatc ctcgaacgcg
     5521 ccgtcgccct gtgcgtcgga tatacagtgc aaatcgacga cctgcaaatc caagatgtgc
     5581 accacaaacc cgtccggacg gaaaccgccg tccccgttgc cgataccttg ccgtctgaaa
     5641 cagccgccgc accgtcccgc cttctcccgt tcgaccccga taccatgcag atacaggact
     5701 atctcgacaa aatcgaacgc gacatcatcg gacaagtcct caaacaaacc gaaggcaacc
     5761 gcacgcaagc cgccaaacgc ttgggcatca gcttccgctc tatgcgctac cgtatggaac
     5821 gcctcaacat agactgacaa acggcatccg caccatctcc gcccacccga aaaaatgccg
     5881 tctgaaacgg cacggggaag cgggttcgcc ccacgaccga acggacacaa tacaccatga
     5941 ccgacatcct cattgacgat accgccaccg aagccgtccg caccctgata cgggcattcc
     6001 ccctcgtgcc cgtttcccaa ccgcccgaac aaggcagtta cctccttgct gaacacgata
     6061 ccgtcagcct caggcttgtc ggggaaaaaa gcaacgtcat cgtcgatttt acctccggcg
     6121 cggcacaata ccggcgcaca aaaggcggag gagaactcat cgccaaagcc gtcaaccaca
     6181 ccgcgcaccc caccgtatgg gatgcaaccg caggattggg gcgcgacagc ttcgtcctcg
     6241 cctcgctcgg actgaccgtt accgccttcg agcaacatcc cgccgtcgcc tgcctgcttt
     6301 cagacggcat ccgccgcgcc ctcctcaatc ccgaaacgca agacaccgcc gcgcgcatca
     6361 acctccattt cggcaatgcc gccgaacaaa tgcctgccct tgttaaaaca caaggcaaac
     6421 ccgacatcgt ttatctcgac ccgatgtatc ccgaacgccg caaaagtgcc gccgtcaaaa
     6481 aagaaatggc ttatttccac cggcttgtcg gcgaagcgca agatgaggtc atcctccttc
     6541 ataccgcacg ccaaacggca aaaaaacgcg tcgtcgtcaa acgcccgcgc ctcggcgaac
     6601 accttgccgg acaagccccc gcctaccaat acaccggcaa aagcacccgc ttcgacgttt
     6661 acctgcccta cggagcggac aagggataac gcccataaaa caagacaccg aaaaatttgc
     6721 cgttcttatg caaacgagaa accggttttt gcgtttcgac tgttttggat aagtcatcac
     6781 accttaaagt ttgtcattcc cacggaagtg ggaatccgat tcgttcagtt ttagttgttt
     6841 tcaagtttca ggcaacttcc aaaccgtcat tcccgcgaaa gtgggaatcc ggaaatgaaa
     6901 ggcaacagga atttatcgta aatgactgaa accgaacgga ctagattccc gcctgcgcgg
     6961 gaatgacggg gcgggaggat gccgtctgaa attccgtcat tcccgtgaaa acgggaatct
     7021 agaacttctg atttttcaga cgacttttga agattgccgc tacccaatga tctggattcc
     7081 cgcttttgcg ggaatga
//
LOCUS       DBJGGI010000084         7046 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.084, whole genome shotgun sequence.
ACCESSION   DBJGGI010000084 DBJGGI010000000
VERSION     DBJGGI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.084"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            80..2245
                     /gene="fetA"
                     /locus_tag="ACSIGJ_001868"
                     /gene_synonym="frpB"
     CDS             80..2245
                     /gene="fetA"
                     /locus_tag="ACSIGJ_001868"
                     /gene_synonym="frpB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor FetA/FrpB"
                     /protein_id="HHQ1330677.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFAGNNGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTTFGDGDHRIVLSHMKDQHRGIRTVREEFAVGGENSRITIKR
                     QAPAYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVKGPNH
                     TRIATRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNGEFKISDKEKGPNGTEVD
                     VDVAQKAKNRANEKIVYAYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKTH
                     DGKTVSSSSLNPSFGVIWQPHEHWSFSASHNYAGRSPRLYDALQTHGKRGIISIADGT
                     KAERARNTEIGFNYNDGTFAANGSYFRQTIKDALANPQNRHDSVAVREAVNAGYIKNH
                     GYELGASYRTGGLTAKVGVSRSKPRFYDTHPKKLLSANPEFGAQTGRTWTASLAYRFK
                     NPNLEIGWRGRYVQKATGSILAAGQKDRDGKLENVVRQGFGVNDVFANWKPLGKDTLN
                     VNLSVNNVFDKFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
     gene            2397..3362
                     /locus_tag="ACSIGJ_001869"
     CDS             2397..3362
                     /locus_tag="ACSIGJ_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HHQ1330678.1"
                     /translation="MLRLTALTLCTVLALGACSPQNSDPAPQAKEQAVSAAQSESASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVVLVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDESIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQIADAFNAAK"
     gene            3380..3571
                     /locus_tag="ACSIGJ_001870"
     CDS             3380..3571
                     /locus_tag="ACSIGJ_001870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330679.1"
                     /translation="MPSETRMQTASCVSDGIARYGRYRGNGGKRFDWNLPHPLPVRRF
                     QTRLSAPTVRTALFESPTP"
     gene            3590..4558
                     /locus_tag="ACSIGJ_001871"
     CDS             3590..4558
                     /locus_tag="ACSIGJ_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHQ1330680.1"
                     /translation="MTAKPFSLNLANLLLPAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGSSMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     KNEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIVSRLMGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            4548..5522
                     /locus_tag="ACSIGJ_001872"
     CDS             4548..5522
                     /locus_tag="ACSIGJ_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HHQ1330681.1"
                     /translation="MPSEKNIGFMAGSSRPLRVAFALLLVSCILFMTVNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYTSLPLTGKFGFELVVMMGGSLLLFYTLIRQGGRDLPHMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVRSELLGIGALVLLVSAAVVWRERYRSDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            5567..6193
                     /locus_tag="ACSIGJ_001873"
     CDS             5567..6193
                     /locus_tag="ACSIGJ_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HHQ1330682.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGTRDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLTLRRKDNGKTVAVSLNAALQPFAPQ
                     MRDIMPKAVGGSASAKELERFGQLWQARVKAFLTESADDPQFVIVREL"
     gene            6277..7035
                     /locus_tag="ACSIGJ_001874"
     CDS             6277..7035
                     /locus_tag="ACSIGJ_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHQ1330683.1"
                     /translation="MITIRNVSYRIGTHPILDNVSLDIPEGGITALIGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLAGTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTHEHKRTTVAVLHDINQAAAYADHVVAMKNGKAALTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
BASE COUNT         1616 a         2128 c         1833 g         1469 t
ORIGIN      
        1 ttattattat tttttcttat cctgccaaac cttaacggtt tggcttaact tcccttcata
       61 cactcaaaag gacgaacaaa tgaacgcccc gtttttccgc ctcagcctgc tctcgctcac
      121 acttgccgcc ggctttgccc acgcggcaga aaataatgcc aatgtcgcat tggataccgt
      181 taccgtaaaa ggcgaccgcc aaggcagcaa aatccgtacc aacatcgtta cgcttcaaca
      241 aaaagacgaa agcaccgcaa ccgatatgcg cgaactctta aaagaagagc cctccatcga
      301 tttcggcggc ggcaacggca cgtcccaatt cctgacgctg cgcggtatgg gtcagaactc
      361 tgtcgacatc aaggtggaca acgcctattc cgacagccaa atcctttacc accaaggcag
      421 atttattgtc gatcccgctt tggttaaagt cgtttccgta cagaaaggcg cgggttccgc
      481 ctctgccggt atcggcgcga ccaacggcgc gatcatcgcc aaaaccgtcg atgcccaaga
      541 cctgctcaaa ggcttggata aaaactgggg cgtgcgcctc aacagcggct ttgccggcaa
      601 caacggcgta agctacggcg caagcgtatt cggaaaagag ggcaacttcg acggtttgtt
      661 ctcttacaac cgcaacgatg aaaaagatta cgaagccggc aaaggcttcc gcaatgtcaa
      721 cggcggcaaa accgtaccgt acagcgcgct ggacaaacgc agctacctcg ccaaaatcgg
      781 aacaaccttc ggcgacggcg accaccgcat cgtgttgagc catatgaaag accaacaccg
      841 gggcatccgc actgtgcgtg aagagtttgc cgtcggcggc gaaaattcac ggataactat
      901 taaacgccaa gcccctgcct accgcgaaac cacacaatcc aacaccaact tggcgtacac
      961 cggcaaagat ttgggctttg tcgaaaaact ggatgccaac gcctatgtgc tggaaaaaaa
     1021 acgctattcc gccgatgaca aagataacgg ctacgcaggc aatgtaaaag gccccaacca
     1081 tacccgaatc gccactcggg gcatgaactt caacttcgac agccgccttg ccgaacaaac
     1141 cctgttgaaa tacggcatca actaccgcca tcaggaaatc aaaccgcaag cgtttttgaa
     1201 cggcgaattt aagatctccg ataaggaaaa aggacccaac ggaacagaag tcgacgtaga
     1261 tgtagcacaa aaagcaaaaa accgtgcaaa tgaaaaaatt gtttacgcct acaaactttc
     1321 caacccgacc aaaacggata ccggcgcgta tatcgaggcc attcacgaga ttgacggctt
     1381 taccctgacc ggcgggctgc gttacgaccg cttcaaggtg aaaacccacg acggcaaaac
     1441 cgtttcaagc agcagcctca acccgagttt cggcgtgatt tggcagccgc acgaacactg
     1501 gagcttcagc gcgagccaca actacgccgg ccgcagcccg cgcctgtatg acgcgctgca
     1561 aacccacggc aaacgcggca tcatctcgat tgccgacggc accaaagccg aacgcgcgcg
     1621 caataccgaa atcggcttca actacaacga cggcacgttt gccgcaaacg gcagctactt
     1681 ccggcagacc atcaaagacg cgcttgccaa tccgcaaaac cgccacgact ccgtcgccgt
     1741 ccgcgaagcc gtcaacgccg gctacatcaa aaaccacggt tacgaattgg gcgcgtccta
     1801 ccgcaccggc ggcctgaccg ccaaagtcgg cgtaagccgc agcaaaccgc gcttttacga
     1861 tacccatcct aaaaaactgt tgagcgcgaa ccccgagttt ggcgcacaaa ccggccgcac
     1921 ttggacggcc tcccttgcct accgcttcaa aaacccgaat ctggaaatcg gctggcgcgg
     1981 acgctatgtt caaaaagcta cgggttcgat attggcggca gggcaaaaag accgcgacgg
     2041 caaattggaa aacgttgtac gccaaggttt cggtgtgaac gacgtcttcg ccaactggaa
     2101 accattgggc aaagacacgc tcaatgtcaa tctttcggtt aacaacgtgt tcgacaagtt
     2161 ctactatccg cacagccaac gctggaccaa taccctgccg ggcgtgggac gtgatgtacg
     2221 cctgggcgtg aactacaagt tctaaaacgc acatcccgaa aaaatgccgt ctgaaagcct
     2281 ttcagacggc atctgtcctg ataatttgat atagattatc atttatcctt tctaaagccg
     2341 ttccggtttg tccgaccggc ggctttgccc caatatcccc attttggaga caccctatgt
     2401 tgcgtttgac cgcattgacc ctctgcaccg tcctcgcctt gggcgcgtgt tcgccgcaaa
     2461 attccgaccc tgccccacaa gccaaagaac aggcggtttc cgccgcacaa tccgaaagcg
     2521 cgtccgttac cgtcaaaacc gcgcgcggcg acgttcaaat accgcaaaac cccgaacgca
     2581 tcgccgttta cgatttgggt atgctcgaca ccttgagcaa actgggcgtg aaaaccggtt
     2641 tgtccgtcga taaaaaccgc ctgccgtatt tagaggaata tttcaaaacg accaaacctg
     2701 ccggaacttt gttcgagccg gattacgaaa cgctcaatgc ctacaagccg cagctcatca
     2761 tcatcggcag ccgcgcagcc aaggcgtttg acaaattgaa cgaaatcgcg ccgaccatcg
     2821 agatgaccgc cgataccgcc aatctcaaag aaagtgccaa agagcgtatc gacgcgctgg
     2881 cgcaaatctt cggcaaacag gcggaagccg acaagctgaa ggcggaaatc gacgcgtctt
     2941 ttgaagcggc gaaaaccgcc gcgcaaggca aaggcaaagg tttggtggtt ttggtcaacg
     3001 gcggcaagat gtccgccttc ggcccgtctt cacgactggg cggctggctg cacaaagaca
     3061 tcggcgttcc cgctgtcgat gaatcaatta aagaaggcag ccacggccag cctatcagct
     3121 tcgaatactt gaaagagaaa aatcccgact ggctgttcgt cctcgaccgc agcgcggcca
     3181 tcggcgaaga gggtcaggcg gcgaaagacg tgttgaacaa tccgctggtt gccgaaacga
     3241 ccgcatggaa aaaaggacag gtcgtttacc tcgttcccga aacttatttg gcagccggcg
     3301 gcgcgcaaga gctgctgaac gcgagcaaac agattgcgga tgcttttaac gccgcgaaat
     3361 aatgaaacgg cggcattcga tgccgtctga aacacggatg caaaccgctt cctgtgtttc
     3421 agacggcatt gcccgatacg ggcgttatcg cgggaatggc ggcaagcgtt ttgattggaa
     3481 cttgccccat cctctgcccg tccggaggtt tcaaacaagg ctttccgctc cgacggttcg
     3541 gactgccttg tttgaatctc ctacgccgta acgcttttcc cttctgttta tgactgccaa
     3601 acctttttcc ctcaacctgg ccaacctcct gctgccggcg gtgttgtttg ccgtcagcct
     3661 gtcggtcggc gttgccgatt tccgctggtc ggatgtgttt tcgctgtccg acagccaaca
     3721 ggtcatgttc atcagccgcc tgccgcgcac gtttgcgatt gtgctgacgg gctcgtcgat
     3781 ggcggtggcg gggatgatta tgcagattct gatgcgcaac cgttttgtcg agccttctat
     3841 ggcgggtgcg ggccaaagtg cggctttggg tttgcttctg atgtccctgc tgctgcctgc
     3901 cgcgccgctg ccggtcaaaa tgtcggttgc cgccgttgcc gcgctgatcg ggatgttggt
     3961 ctttatgctg ctgatccgcc gcctgccgcc gacggcgcaa ctgatggtgc cgctggtggg
     4021 gattattttc ggcggcgtgg ttgaggcggt ggcgacgttt atcgcgtata aaaacgaaat
     4081 gctgcaaatg ctcggcgtgt ggcagcaggg cgatttttcg agcgtgctgc tggggcggta
     4141 cgagctgctt tggattacgg gcggtttggc ggtgtttgcc tacctgattg ccgaccggct
     4201 gacgattttg gggctgggcg agacggtcag cgtgaatttg ggtttgaacc ggacggcggt
     4261 gttgtggtcg ggtttgatta ttgtggcact gattacatcg ctggtcattg taacggtcgg
     4321 caatattccg tttatcgggc tggtcgtgcc gaatatcgtc agccgcctga tgggcgacag
     4381 gctgcgccaa agcctgcctg cggtcgccct cttgggcgcg tctttggttt tattgtgcga
     4441 cattatcgga cgcgtgattg tgtttccgtt tgaaattccg gtctctacgg tttttggtgt
     4501 gttgggtacg gctttgtttt tgtggctttt attaaggaaa cctgcccatg ccgtctgaaa
     4561 aaaatatcgg ttttatggca ggaagcagcc gtccgttgcg ggtcgccttt gcgctgttgc
     4621 tggtttcctg catcctgttt atgacggtca acgtcaaagg cgattgggac tttgtcttgc
     4681 acctgcgcct gaccaagctt gccgcgctgc tgatggtcgc ctatgcggtc ggcgtgtcca
     4741 ctcaactctt ccaaacgctg accaacaacc cgattctgac cccttcgatt ttgggtttcg
     4801 attcgctgta tgtgtttttg cagaccttgc tggtgtttac gttcggcggc gtgggctata
     4861 catccctgcc gttgacgggc aaattcggct ttgaactggt tgttatgatg ggcggctcgc
     4921 tgctgctgtt ttacacgctc atccgtcagg gcgggcgcga tttgccgcac atgattttaa
     4981 tcggcgtgat tttcgggatt ttgttccgca gcctttcctc gctgctttcg cgcatgatag
     5041 accccgaaga atttaccgcc gcgcaggcga atatgtttgc cggattcaat accgtccgca
     5101 gcgagctttt aggcataggc gcgctggtcc tgctcgtcag cgcggcggtc gtttggcgcg
     5161 aacgctaccg ctcggacgta caccttttgg ggcgcgacca agccgtcaat ttgggcatca
     5221 gctacacgcg caacacctta tggatactgc tttggattgc cgcattggtg gcgacggcga
     5281 ccgccgtggt cggcccggta agctttttcg ggcttctcgc cgcctcgctt gccaaccact
     5341 tttccccgtc cgtgcgccat tccgtccgcc tgccgatgac ggtttgcgtc ggcggcatcc
     5401 tcttggtcgg cggacaaacc gtattcgaac acttcttggg catgaaggcg gtattaagcg
     5461 tggtggtcga atttgcgggc ggactcgttt tcctctatct cgttttaaaa cacaaaaaat
     5521 gacggatgcc gtctgaacgg cagcccgccc cgaaaggaca aaccatatga cgcaagaacg
     5581 tttacccgaa tttttcgacc gcgccccgac gctgaccgta caagacccgc ttgccgcatt
     5641 cctcggcgcg gccgaaaacg gcatcctcac ttaccgctac gccgatgccg tgcgcctgtg
     5701 cggacattcc tgcccgaccg tcgcgggcgc gtacctgatg gttatcaaag gcctgaaagc
     5761 actttacggc gaagagctgc ccgaacgcgg cggcatcgaa gccgccatgc agggaacgcg
     5821 cgacgaaggc acggtcggcg taaccgcgtc cgtcgtccaa ctccttaccg gcgcagcccc
     5881 cgaaaccggc ttcggaggca tcggaatgca gggacgcttc gcccgccgca acctcttatc
     5941 ctttggtgca ggcgaaatca acggcacact gaccctgcgc cgcaaagaca acggcaaaac
     6001 cgttgccgtc agcctcaacg ccgccctgca accctttgca cctcaaatgc gcgacatcat
     6061 gcccaaagcc gtcggcggca gcgcaagcgc aaaagaactc gaacgcttcg gacaactttg
     6121 gcaggcacgc gttaaagcat ttttgaccga atcggcggac gacccgcagt tcgtcatcgt
     6181 ccgcgaactg tgaacgttca gacggcattc cgaattccaa atgccgtccg aacccccgcc
     6241 aaacaacaac aaacctacgc ccgacaagca tccgccatga ttaccatccg caacgtcagc
     6301 taccgcatcg gcacacaccc catcctcgac aacgtcagcc tcgacatccc cgaaggcggc
     6361 attaccgcgc taatcggccc caacggtgcg ggcaaatcca ccctgttttc ctttatggcg
     6421 cggctgcgcc cgcttgaaag cggcagcatc gcctaccgag gcaaaaacct tgccggcacc
     6481 cccaccgccg aactcgcccg cacgctctcc atcctcaccc aagaaaacag catcatgagc
     6541 cgcatcaccg tgcgcgacct gctgatgttc ggccgttacc cctaccatca aggcagaccg
     6601 actgccgaat gccgccgtat cgttaacggt gcaatcgaag aattccacct gcaggacctc
     6661 tccgaccgct acctgaccga actctccggc ggacaacgcc aacgcgccat gattgcgatg
     6721 gtgttctgcc aaagcaccga ctacgtcctt ttggacgaac cgctgaacaa cctcgatatg
     6781 tatcacgccc gctcgctcat gcagattctg caaaagctca cgcacgaaca caaacgcacc
     6841 accgtcgccg tattgcacga catcaaccag gccgccgcct acgccgacca cgtcgtcgcc
     6901 atgaaaaacg gcaaagccgc cctgaccggc acacccgaag aagtgttcac cgtacacaac
     6961 atcaaagaac tgttcgacat ggacgtggac gtactcgact acgaaggcaa aaaactcatc
     7021 gtccaccaca tctgaagctc aaaaag
//
LOCUS       DBJGGI010000085         7006 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.085, whole genome shotgun sequence.
ACCESSION   DBJGGI010000085 DBJGGI010000000
VERSION     DBJGGI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.085"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            394..798
                     /locus_tag="ACSIGJ_001875"
     CDS             394..798
                     /locus_tag="ACSIGJ_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HHQ1330684.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWHG
                     HIAEITDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DATFPAPIWANPVRKHGMTLPQNWTEQLKKLL"
     gene            838..2019
                     /locus_tag="ACSIGJ_001876"
     CDS             838..2019
                     /locus_tag="ACSIGJ_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HHQ1330685.1"
                     /translation="MTEPTSRRRFLKTCTAAGAGLLQACGTSATSVPPLPSSHSVVKA
                     RTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRFQ
                     RFAGTDAQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGTL
                     FFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGTTQNRLTVDV
                     PYIQRADVETEGTLWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNTL
                     YLSGILGKQRAIVFGDFRMEKIRDLYDSSYDFSAVAKHISRTAKIPVLTGFPFGHIAD
                     KITFPLGAHTRIRMNGNGGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADIS
                     E"
     gene            complement(2121..4377)
                     /locus_tag="ACSIGJ_001877"
                     /pseudo
     CDS             complement(2121..4377)
                     /locus_tag="ACSIGJ_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
     gene            4750..5928
                     /gene="nirK"
                     /locus_tag="ACSIGJ_001878"
     CDS             4750..5928
                     /gene="nirK"
                     /locus_tag="ACSIGJ_001878"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HHQ1330686.1"
                     /translation="MKRQALAAMIASLFALAACGGEQAAQAPAETPAASAEAASSAAQ
                     ATAETPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWT
                     FDGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTST
                     FSFKALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKG
                     KKGAQGLQPFDMDKAVAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLV
                     SSFHVIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRA
                     FNKGALGQLKVEGAENPEIMTQKLSDTAYAGSGAASAPAASAPAASAPAASASEKSVY
                     "
     gene            6106..6861
                     /locus_tag="ACSIGJ_001879"
     CDS             6106..6861
                     /locus_tag="ACSIGJ_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HHQ1330687.1"
                     /translation="MKYVRLFFLGTALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNISWFAANAYCAAQGKRLPTIDEWEFAGLASATQRNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASVGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCASR"
BASE COUNT         1770 a         1977 c         1756 g         1503 t
ORIGIN      
        1 aatgacggga ttttagtaac cgtaacaact gcctgcgcga cggctaaggg gcttcagcaa
       61 ccgtagcaac tgcctgtgtg ggaatgacgg acaatgggct tcagacggca tctcttgcct
      121 tccgctaaaa cagtttgccg cacaactgtt caaacgcgtc cgatatgttt caacacacag
      181 gacgacacat aaagcaccgc cctatatgtc gtcctgattc ggaaggggtt acgcccctcc
      241 caaacaaagt ctgatcctgc cgccctaaag ggcggggttt caaccgaaaa ggaaacacga
      301 tgaagtggta caattaacgg caatgcggac agacaaatta aactatcaga aattgcccta
      361 ccgtttttta aacactttca ggaataagga aaaatgaccg cccaaccctg ccccatctgc
      421 acggcacaag atgaagatat tttgctgcaa acccccaagc tgcgcgtcat cgccgtccat
      481 aacgacagcg gttcgccggc attctgccgc gtcatttggc acggacacat tgccgaaatt
      541 accgaccttt cggcagcgga acgcggcgaa ttgatggaaa tggtgtacaa agtcgaagcc
      601 gctatgcgcc aagtgttccg gccggcaaaa atcaacctcg ccagcttggg caatgtcgtg
      661 ccgcacctgc attggcatat tatcgcccgc tttgaaaacg atgcgacttt ccctgcgccg
      721 atttgggcaa accccgtccg aaaacacggt atgaccctgc cgcaaaattg gacggaacag
      781 cttaaaaagc tgctttaaac ccgccgatgc cgtctgaaac cgtatgaaag ggaaattatg
      841 accgaaccga cctcccgccg ccgttttctg aaaacctgca ccgccgccgg cgcggggctg
      901 cttcaggctt gcggcacatc cgccacatcc gttccgcccc ttccctcttc ccattccgtt
      961 gtgaaagccc gaaccgtgcc tctccaaacg ccacgccgtc aaagttcgga cggcaacctt
     1021 ctgcgcgttg tcgcttcgtc aggatttgcc gaagacacta accgcgtcaa cacagcctta
     1081 acccgccttt acaatgccgg ttttaccgta accaaccaac aggcgggcag ccgccgtttc
     1141 caacggtttg ccggcacgga cgcgcaacgc gcggcagatt tccaagaggt cgcctccggc
     1201 cgcgtcgcca cgcctaaagt gctgatgggc ttgcgcggcg gttacggtgc ggcgcggatt
     1261 ctgccgcata tcgattttgc ctcgctcggc gcaaggatgc gcgaacacgg cacgctgttt
     1321 ttcggattca gcgacgtatg cgccgtccaa ctggcattgt tggcaaaagg caatatgatg
     1381 agttttgccg gcccgatggc ttatagcgat tttggcaaac ctgcccccgg tgcgtttacg
     1441 atggatgcct ttatcaaggg gacaacccaa aaccgcctga ccgttgatgt tccttatatc
     1501 caacgcgccg atgtcgaaac cgaaggcacg ttgtggggcg gcaacttaag cgtcctcgcc
     1561 tcgctcgccg gtacgcctta tatgcccgac atcgacggcg gcattttgtt cctcgaagat
     1621 gtcggcgaac agccctaccg catcgaacgt atgctcaata cgctgtatct ttcgggtatt
     1681 ttgggcaaac agcgcgccat cgtgttcggc gatttccgta tggaaaaaat tcgagatctc
     1741 tatgattcgt cttatgattt ttccgccgtt gccaagcata tttcgcgcac ggcgaaaatc
     1801 cccgtgctga cgggcttccc gttcggacac attgccgaca aaatcacttt ccctctgggc
     1861 gcgcacaccc gaatccgtat gaacggaaac ggcggctatt cggtcgcgtt tgaaggctac
     1921 cccacactcg atgcgtccgc cctgactttg gataccctgc tcccaccgcc ggatttgcct
     1981 atcttccccg aaagcggtgt tgccgatatt tcggaataaa cccgcaaacg gacaaatgcc
     2041 gtctgaagcc ttcagacggc attttcctgt aaaaaaaccg cacctgatgg caggtgcggt
     2101 ttccattttc aggctaaaac ttatttgtcg cggccgaata cgattttagt ggcttggatg
     2161 gcaacgcaga ttgcaccgcc gataaagatt aagtcggggg cagtgcgtac ccagcgcagg
     2221 gtgtcgagga tttccatttg caggaattct tcgctgcggg cataccacaa accgtgcgtg
     2281 atagaggcgt atgcctgaat cacgccgaca ggcagcaggc tgatggcaat cataccgacc
     2341 aagccgccgt tgagcagcca gaagccccaa gtcatcagtt tgtcgtcaaa ccgcgcgttc
     2401 ggtttcaagt agcgcgcaac caacaatacg aagcccaatg ccaagaaacc gtacacaccg
     2461 aacaaggcgg cgtgcgcgtg aacggcggaa gtgttcaaac cttggatata gaacagggaa
     2521 atcggcggat tgatcagaaa gccgaatacg ccggcaccga tcatattcca aaaagcgact
     2581 gccacgaagc acatcagcgg ccaacgcagg cgtttcgccc agtcggacag gtgttggtaa
     2641 gaccagtgct cgtatgcctc gcggcccagc aacaccagcg gcacgacttc caaagcggag
     2701 aagcaggcgc cgattgccat agaggcggag gtagagccgg agaagtacag gtggtgcagc
     2761 gtgcccggaa cgccgcccaa cataaagatg gcggcagcgg ccaaagtgga ggcagtggcg
     2821 gtactgcggc ggacaaagcc catattgtag aagacaaagg caaaggcggc agtggcaaat
     2881 acttcaaaga agccttccac ccacaggtgg accacccacc aacgccagta ttccattacg
     2941 gcaatcgggg atttttcgcc atagaacagg cccggtgcgt agaacacgcc cacgccgacc
     3001 ataagaagcg acaaagattg ccagcaagtt tttgtccacg cctttttctt tgaaggcgga
     3061 aaccgtgcag cgcaacatca ggaacagcca caacagcagt ccgaccatca aaaggagttg
     3121 ccagaaacgt cccaaatcga ggtattcgta accttggtgt ccgaaccaga agttaaattc
     3181 gggaggaagg atgtgcgtca acgcgaagaa gttgcccgcg taagaaccgc cgaccacgat
     3241 gaagagggcg atatagagga agttcacgcc tgcacgttgg aacttgggat ctttgccgcc
     3301 gttgacaatc ggcgcgagga acaaacctgc cgtcaaaaag ccggttgcaa tccagaagat
     3361 ggcggattgg atgtgccaag tacgggtcag ggcataggga aaccagtcgg acatttcaaa
     3421 gcccaacgcc tcgtcaatgc cgtagaaacc ctgaccttcg acggtgtagt gcgcggtcag
     3481 gccgcccagc aatacttgta ccacaaacag ggcgaccgtc aggaagacgt atttgcccaa
     3541 tgctttttgc gaaggggtca gttggatttt ggaaatcggg tcttcagacg gcacttccac
     3601 ttcctcgtgt ttggtcagga aggaataacc ccacatcaac aaaccgatgc ccatcaagag
     3661 cagtacgaca ctggtaaacg accacatgta gttttcagta gtcggtacat tgttgatcaa
     3721 aggctcgtgc ggccagttgt tggtgtaggt aaagacctcg ccgggacggt tggtcgaagc
     3781 agaccaagaa gtccagaaga agaagtcgaa cagtttttca cgcgcttctt ggcttggcaa
     3841 tgtgttgttt ttcattgcaa agtgttcgcg ggtggtttgc aacttgggat catcgccgta
     3901 aacgccgtga tagtaaggca ggatgctttc gatggctttc acgcgcgtat cgctgatgac
     3961 gacgctgccg tcttctttaa tacggctttg attgcggtat tcatcggcca agcgggtttt
     4021 cagaacggct tgttcttcag gggaaacttc atcgaatttt ttgccgtaag cctgttgcgc
     4081 ggtcaaatcc aaccaggcgg acaactcacg atgcagccag tcggccgtcc agtccggagc
     4141 ctgatatgcg ccgtgtccca gaatcgaacc gacttccatg ccgccggtag tctgccacgc
     4201 agactgacct gccaaaatat cgtctttcgt catcagcact ttgccggatg cggaaacgac
     4261 ctgttcgggg taaggcgggg ctttcttata aacctcgctg cccatatagc caagaatggt
     4321 aaagcatacc gccagaacgg caaacagcaa gtaccacagc ttcttgtact gtcccatttt
     4381 gagagctcct tttaataaat cagttgttta aaattcacaa aatatgaatg ttaaagattg
     4441 tagcatggtt taccgcgcaa ataaacattt gttcaaacaa actcacatat agaacaaata
     4501 catatatgat aataactatc attattcttt actcggaaat tgccctgcct ttgcctgctc
     4561 tgccggagcc cctagcaaat cagcctattc attgtaattt ttagtagtta taaagtatta
     4621 gaagcgtcat tttaagttca tattttatga attatttgac ttaaattaaa atgcccgcaa
     4681 tgggacaacc gcataatcac accaagttct taactaatcc ccctactttt cttacaaaag
     4741 gaaaacatta tgaaacgcca agcattagcc gcaatgattg cttccttatt cgcattggcc
     4801 gcctgcggtg gcgaacaagc tgctcaagcc cctgccgaaa cccctgccgc ttccgcagaa
     4861 gccgcaagtt ccgccgcaca agctaccgcc gaaacgcctg caggcgaact gcccgtcatc
     4921 gatgcggtga ccacccacgc tcccgaagta cctcccgcaa tcgaccgcga ctatcctgcc
     4981 aaagtacgcg taaaaatgga aaccgtcgaa aaaaccatga aaatggacga cggggtggaa
     5041 taccgctact ggacatttga cggcgacgtt ccgggccgta tgatccgcgt acgcgaaggc
     5101 gatacggttg aagtcgaatt ctccaacaat ccttcttcta ccgttccgca caacgtcgac
     5161 ttccacgccg caaccggtca gggcggcggt gcagccgcga cctttaccgc cccgggccgc
     5221 acttccacat tcagcttcaa agccctgcaa ccgggcctgt acatctacca ctgcgccgtc
     5281 gcgccggtcg gtatgcacat cgccaacggt atgtacggtc tgattttggt cgagcctaaa
     5341 gaaggcctgc cgaaagtgga taaagagttt tacatcgtcc aaggcgactt ctacaccaaa
     5401 ggcaaaaaag gcgcgcaagg cctgcaaccg ttcgatatgg acaaagccgt tgccgaacag
     5461 cctgaatacg tcgtattcaa cggccacgta ggcgctatcg ccggcgacaa cgccctgaaa
     5521 gcgaaagcag gcgaaaccgt gcgtatgtac gtcggtaacg gcggcccgaa cttggtgtct
     5581 tccttccacg tcatcggcga aatcttcgac aaagtttatg ttgaaggcgg caaactgatt
     5641 aacgaaaacg tacaaagcac catcgtgcct gccggcggtt ctgccatcgt cgaattcaaa
     5701 gtcgacatcc cgggcagcta cactttggtc gaccactcca tcttccgcgc attcaacaaa
     5761 ggcgcgttgg ggcaattgaa agtagagggt gcggaaaacc ctgaaatcat gactcaaaaa
     5821 ttgagtgata ccgcttacgc cggcagcggc gcggcttctg cccctgctgc ttccgcaccg
     5881 gctgcttctg ccccggcagc ctctgcatcc gaaaaaagcg tttattaaat cggatacccg
     5941 tcattagcgg gacaaaccac tgccgccgta cttcattacg cacggcagtg gttttttaac
     6001 aaccaatctt tcctttcgga agattgattt ttcagacggc attcgcggct ttttgtttaa
     6061 aatgccgtct gaaaaatcaa tgctaaccgc ctgtcaggag gctttatgaa gtacgtccgg
     6121 ttatttttcc tcggcacggc actcgccggc actcaagcgg cggctgccga aatggttcaa
     6181 atcgaaggcg gcagctaccg cccgctttat ctgaaaaaag ataccggcct gattaaagtc
     6241 aaaccgttca aactggataa atatcccgtt accaatgccg agtttgccga atttgtcaac
     6301 agccaccccc aatggcaaaa aggcaggatc ggttccaaac aggcagaacc cgcttacctg
     6361 aagcattgga tgaaaaacgg cagccgcagc tatgcgccga aggcgggcga attgaaacag
     6421 ccggttacca atatttcctg gtttgccgcc aacgcctatt gcgccgcaca aggcaaacgc
     6481 ctgccgacca ttgacgaatg ggaatttgcc ggacttgctt ccgccacgca gagaaacggc
     6541 tcaaacgaac caggctacaa ccgcactatt ctcgattggt atgccgacgg cggacggaaa
     6601 ggcctgcacg atgtcggcaa aggccgcccg aactactggg gcgtttacga tatgcacggt
     6661 ctgatttggg aatggacgga agatttcaac agcagcctgc tttcttccgg caatgccaac
     6721 gcccaaatgt tttgcagcgg cgcatctgtc gggtcgagcg actcgtccaa ctatgccgcc
     6781 ttcctccgct acggcatccg taccagcctg caatccaaat acgtcctgca caacttaggc
     6841 ttccgctgcg caagccgata accccttcaa ttatagtgaa ttaacaaaaa ccagtacggc
     6901 gttgcctcgc cttgccgtac tggtttttgt taatccgcta tattccgcca tctctaagat
     6961 ttacagcgat acacgggtga tttaaggaat gcccgaaccg tcattc
//
LOCUS       DBJGGI010000086         6896 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.086, whole genome shotgun sequence.
ACCESSION   DBJGGI010000086 DBJGGI010000000
VERSION     DBJGGI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.086"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(<1..749)
                     /locus_tag="ACSIGJ_001880"
     CDS             complement(<1..749)
                     /locus_tag="ACSIGJ_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HHQ1330688.1"
                     /translation="MNSFIKSTPIVLILILCSSNSYSEPVRLEKSQIKFQSSNNLKSI
                     GFKLDSSSNTFSKFTEAANFEHIPTGAKARINAKITASVSRAGVLSGVGKLVRQGAKF
                     GTRAVPYVGTALLAHDVYETFKEDIKARGCRYDPETDKFVKGYEYANCLWYEDERRIN
                     KTYGCYGVDSSIMRLMPDRSRFPEVKQLMESQMERIARPFWDKRKEEIPKDTALKWRL
                     FPFSHCEFFWNGGGCFVRNGDNLIHRVYFKL"
     gene            complement(691..1008)
                     /locus_tag="ACSIGJ_001881"
     CDS             complement(691..1008)
                     /locus_tag="ACSIGJ_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HHQ1330689.1"
                     /translation="MNKPFITQAQLALYKYQPSSEYFGQSMAFIAQKEFEEFVNNVKE
                     YDILESFSYFLNKRVAHNIWKIYFSDESVIFIRKSEENGKTVHEFVYQEYTDSSDFNS
                     MFE"
     gene            complement(1136..1414)
                     /locus_tag="ACSIGJ_001882"
     CDS             complement(1136..1414)
                     /locus_tag="ACSIGJ_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330690.1"
                     /translation="MYCQVGNKCLEKHRAENLYFSLVVPRIQENGQIIRPEYNGSMWK
                     MSDGQPLRLSLAECSPKDNLQSGLETGRIVFGVLASVYFVSLLKKVLK"
     gene            complement(1421..1639)
                     /locus_tag="ACSIGJ_001883"
     CDS             complement(1421..1639)
                     /locus_tag="ACSIGJ_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HHQ1330691.1"
                     /translation="MKFINTCRKYGAKLAVVTAAPLALAAQANAALPETAKNALEAAK
                     ADGMEAGWIVVGVFAALFVFSIVKRVMK"
     gene            complement(1713..1910)
                     /locus_tag="ACSIGJ_001884"
     CDS             complement(1713..1910)
                     /locus_tag="ACSIGJ_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330692.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAV
                     NAGLEEIGNQCEFVVFGFLKD"
     gene            complement(1915..2205)
                     /locus_tag="ACSIGJ_001885"
     CDS             complement(1915..2205)
                     /locus_tag="ACSIGJ_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330693.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVTGFQFVKKG"
     gene            complement(2250..3598)
                     /locus_tag="ACSIGJ_001886"
                     /pseudo
     CDS             complement(2250..3598)
                     /locus_tag="ACSIGJ_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            complement(3737..4159)
                     /locus_tag="ACSIGJ_001887"
     CDS             complement(3737..4159)
                     /locus_tag="ACSIGJ_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HHQ1330694.1"
                     /translation="MTDIFTPSKRSFVMSKIHSKETKPEVLVRKFLFSQGFRYRKNDK
                     RYAGKPDIVLPKYKTVVFIHGCFWHGHSCNKGHIPKSNMDFWLEKITKNRERDIKNET
                     ELEKIGFKVIVVWECELKNKAICRERLNRLVEEIKDAV"
     gene            complement(4165..4761)
                     /locus_tag="ACSIGJ_001888"
     CDS             complement(4165..4761)
                     /locus_tag="ACSIGJ_001888"
                     /inference="COORDINATES: protein motif:HMM:TIGR02391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HHQ1330695.1"
                     /translation="MKGESGVDIENWKNKLPEKEREPVEVILNRLEDSELTNKEQAEV
                     ISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFEDKVQKQTGL
                     HSIDGRELIEKAFGSKKSMLLLTNNKTQAEQNLEDGLEQLACGTWTGFRNPVQHELRA
                     NLSPSIFNDKDALDLISLVSYLLRKVEQTKKRAKPTSP"
     gene            complement(4951..5808)
                     /locus_tag="ACSIGJ_001889"
     CDS             complement(4951..5808)
                     /locus_tag="ACSIGJ_001889"
                     /inference="COORDINATES: protein motif:HMM:NF024977.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHQ1330696.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIGRNRREEKQASPCGRIPTGKKGLG
                     KLALFRLGNKIEISTIQGNERVTFTLDYAEIKKSERIYQPEFQKESVKPNTENGTTIT
                     LTELTKKQGYPLDNYVGHLSRLFDFPAQDFKIKVSLNGSEPRIIDGNLKYNLVTPQFE
                     WEYQDLATNISSLSSKFEQYEYSGLIQGKFITTEKPLKNNMKGITLFANGRMVNMPEF
                     FTDSESSHF"
     gene            complement(5822..6193)
                     /locus_tag="ACSIGJ_001890"
     CDS             complement(5822..6193)
                     /locus_tag="ACSIGJ_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HHQ1330697.1"
                     /translation="MKRLALIPKNGGNRLAFAHIPELQLECFIGKDNSFKDTFGRLWW
                     DKPAPTITTKFFSISNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIG
                     NAVPPMYAEKIGRAIVDNIEC"
     gene            complement(6190..6798)
                     /locus_tag="ACSIGJ_001891"
     CDS             complement(6190..6798)
                     /locus_tag="ACSIGJ_001891"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HHQ1330698.1"
                     /translation="MQVLAGIDYELSCKETYEANINAKFIQADIFELQPETLEKELGL
                     KKNDDDLILIGCSPCQYWSVIQTDKRKSEKSKSLLLEFQRFVEYFNPGYVVVENVPGI
                     LSRMKESGLDNFIKLLEEKGFTVHFGIHNTADYGIPQSRKRFTLIANRITKKSWNQSS
                     IRANGLRYAMFWEWKTAFPKLWQDIKTRRILCIVVRDYPISI"
BASE COUNT         1806 a         1571 c         1309 g         2210 t
ORIGIN      
        1 tcaagtttaa aataaactcg atgaattaaa ttatcaccgt tacgtacaaa acaaccgccg
       61 ccgttccaaa aaaattcaca atgactaaaa ggaaaaagac gccactttaa agcagtatct
      121 ttaggaattt cttctttccg tttatcccaa aaaggacgag caatcctttc catttgactt
      181 tccatcagtt gtttgacttc ggggaatctg ctgcgatcgg gcataaggcg cataatcgaa
      241 ctgtcaacgc cgtagcagcc ataggtttta ttaatacgtc tttcgtcttc gtaccaaagg
      301 caattagcat attcgtagcc ttttacaaat ttgtcggttt cgggatcgta tcggcagcct
      361 cgtgccttta tgtcttcttt gaaagtttcg tatacgtcgt gggctaaaag ggctgttccg
      421 acatagggaa ccgcccttgt gccgaatttc gcgccttggc ggacaagttt gccgaccccc
      481 gacaatacgc cggcgcggga tacgctggcg gttattttgg cgttgattcg ggcttttgcg
      541 cccgtgggga tgtgctcgaa atttgccgct tctgtgaatt tggaaaaagt gttagaactt
      601 gaatctaatt taaaaccaat tgattttaaa ttattacttg attgaaattt aatttgtgat
      661 ttttctaatc ttacaggttc agaataagaa ttactcgaac atagaattaa aatcagaact
      721 atcggtgtac tcttgataaa cgaattcatg aacagttttt ccgttctctt ctgattttct
      781 tataaaaata acagactcat cagaaaaata aatcttccaa atattatgtg cgaccctctt
      841 atttaagaaa taagagaagc tctctaaaat atcgtactct tttacgttat taacaaattc
      901 ttcaaattct ttctgagcaa taaaagccat cgattgccca aaatactcgc ttgacggctg
      961 atatttataa agtgccaact gcgcctgcgt gataaacggc ttgttcatgg ttctgccttt
     1021 caaaggttgt tttgaaagcc tgattttgac accataactt catgcgctca atccttaaac
     1081 agaaccgccc cgattaatac ggggacggca acgccgagat agaaataaaa atccatcatt
     1141 tcaaaacctt tttcagcagg gaaacaaagt aaacggacgc gaggacgccg aatactatcc
     1201 ggcctgtttc aagaccgctt tgcaggttgt ctttcggact gcattccgcc aatgaaagcc
     1261 ttagcggctg accgtccgac atcttccaca tgctgccgtt atattccggc ctgattatct
     1321 gtccgttttc ttggattctt ggtactacca agctgaaata aaggttttcg gcccggtgct
     1381 tctcaagaca tttatttccg acttggcagt acatgccgcc ttacttcatc accctcttaa
     1441 cgatggaaaa tacaaaaagc gcggcgaaaa cgcccactac aatccaaccg gcttccatac
     1501 cgtccgcttt tgcggcttcc aaagcgtttt ttgccgtttc gggcaacgct gcgtttgcct
     1561 gtgccgccaa agccagcggg gcggctgtta caacagccag ttttgcgccg tatttacggc
     1621 aggtgttaat aaatttcatg atattttcct tcaaaaagtg tttggcggta atggatggag
     1681 agtttttcag acgaccgccg aacatccgaa aatcagtctt tcaaaaatcc gaatacgaca
     1741 aattcgcatt ggttgccgat ttcttccaaa cctgcgttaa ccgcttcttc gaagtcgtag
     1801 aaataatcgg cattggtaat taatttggta tgtccgatgt cgcccgtttc aggagagaga
     1861 tacagaaagt cccctgttga tacggactgg acaacataga ctttctgcat tcaatcagcc
     1921 tttcttaacg aattgaaaac cggtgacttt cagtttttga gttttgcccg tagtgacgat
     1981 ttccacgttc aggtttgctt cgatcggaaa ttgggcgttt cggaactgct cgaaattggc
     2041 agagccgccg aaatcgtatt cagtagtaga gctgcccaat gcgttgcctt gggagctgtc
     2101 taagggtgtg gcgacaatca ggcggcaata gtcgaagctc ttgccttcga tttgtccgtt
     2161 gaatttttta acgccgacga tgtggccttg aagttggatg ttcatttttt ggtttccttg
     2221 tgtgattaaa cgtctttcgg gcagacactt taagcccatg aaatcggtag tcttgcgaat
     2281 ttgtcgtaaa tgaagttgtt atagctttct tcattgttga cgtgtttttg ctgttcaagc
     2341 tgtttttcaa gattctcgta atattcgtac atataataag ggtcattgta cggtttgaat
     2401 gcgggctgtt catgaatgac ttgagatttc aaaaaggcgc agtcgtaggc ttcgagagcc
     2461 aaagacttgg gcagcttgtg atgactcggc tcaatcagtt caaacagttt ggctttgtcc
     2521 aattcgggaa aaatgaattt cagaccgttt gccgcacgtc cgaactgttt ttttacccat
     2581 tcaaggcagc ggtcggctga aacaacctta tcttccttaa ccgcgtgtat gcgcgttgcc
     2641 ttttgggcga atcgttcgca aatgggatat gcgccgccga aatattcgcc cggattctgc
     2701 aaaacttcga aagggataac gatgtctttt gctttgaatt caatttcaaa gcgcgtccat
     2761 gtgcttgttt tatcgcccaa ctgcttgcct ttttcataga cgcggacata tttggacgat
     2821 tcacgggagc cgataccata ggtcttgcct ttggtcattt tggcttcatc gtcttcttcc
     2881 caatccgacc ccaaacattc gcccttcggt ttgacgtgat ggcacgtaaa caaaccttta
     2941 ttgcggtctt ctcttgcctg attcgggctg tactctccgt tgaaaaagtc ttttgcaatg
     3001 tcaacgcgcg ttatttttgg acgaatcgca tttgtcagaa aatcaaaaag ccttgattcc
     3061 catccaatat ttgcagcatt gcagcctacc gccgtcaatt caaccaacat cgtttcacgt
     3121 tggccgccgt aatggacgcg cccgtattgg gcattttccg ttcccatcag ccagcagcct
     3181 tcatagaaac gcccgcccga atgcttggct ttttcaatga ttccgaatcc gaaaatttcc
     3241 tccatcttgg cggaagccgc gcgtatgaaa tcgtcatcat cgaacaaatc aaacgaaagt
     3301 ccataaacat ggaaaaacgt gtcttcatga attgaaaatg tgatttggtc aatgaaagcc
     3361 gaatctgata caccgcgcct aagaggaacg cctaacaggt ttcctttacc gtccgttatg
     3421 tacgtttcgt aacattcgaa gacttcctga accctgcccg ccgtttcggt ttctgtgccc
     3481 ccccctgtta gataaggggg gggggaagat tcgaagcggt tgccggcttc ctgccgtccg
     3541 ccggcgcctc cgtcatcacg ccggcaacct cctttgtcat cccttgctta tcttccattt
     3601 gcgaatcctc aaaaacgggc aaaaaaaaag ccctgttact tgtagaaagt aaagggcgtt
     3661 aatttttgtt aatcacccct tcttagggac gcaatatata agggtcttaa ttaaaggaag
     3721 atgccgtctg attttttcag acggcatctt ttatttcctc gaccaaccga tttaatcgct
     3781 ccctacaaat agctttattt tttaattcgc actcccaaac gacaatcact ttgaaaccaa
     3841 tcttttccag ctcggtttca tttttaatat cacgttcgcg attttttgtg attttttcca
     3901 accaaaaatc catattgctt ttcgggatat gtcctttatt acaagaatgc ccgtgccaaa
     3961 aacagccgtg tataaacacg actgttttat atttaggcaa aacgatatcc ggtttgcctg
     4021 catagcgctt atcgtttttc cgataacgga aaccttggga aaaaagaaat ttccttacca
     4081 atacttccgg tttggtttct ttgctatgga tttttgacat cacgaaggaa cgtttggatg
     4141 gagtgaaaat atcggtcata aaatttaagg cgatgtaggt ttagctcgtt tttttgtttg
     4201 ttccactttt ctcaataaat atgaaactaa gctgattaaa tccaaagcat ccttgtcatt
     4261 aaaaatacta ggagataaat ttgctcttaa ctcatgttgc acaggatttc taaatccagt
     4321 ccatgttccg catgcaagct gttcaagacc atcttctaaa ttttgttccg cctgcgtttt
     4381 gttattcgtt aatagaagca tagatttttt actcccgaag gctttttcaa tcaattctct
     4441 tccgtctatg ctatgaagac ctgtctgctt ctgaacctta tcttcgaaaa ctttaactgc
     4501 ctcgattgca gcacttaaat aatccttctt ttcattgtaa aaatcgttac aggcagtatg
     4561 caaatcctga tgcaaatgcc gccaatgata gtaaggatat tctggaataa tactatgtaa
     4621 ggcagagatt acttccgctt gttctttatt tgtaagctcc gaatcttcca aacgattcaa
     4681 aataacttct accggttctc tttccttttc tggcaatttg tttttccaat tttcgatatc
     4741 aactccgctt tcacctttca cttcgtaatc tttctctttt tttcttcttt ttctccaatc
     4801 ttgcccgatg gctcagacga tttccccaag gaaaacccgt aattttgaag ttttaggatg
     4861 cttccaatcc aatgatgtcc tatctgtagc gattaaatct tcaccatcat tatctataaa
     4921 atcaatactt aaccatcctg tcagatagga ttagaaatgg ctggattcgc tatcagtgaa
     4981 aaactcgggc atatttacca ttctgccgtt ggcaaacaag gtaatacctt tcatattatt
     5041 ctttaaaggt ttttccgttg taatgaactt accttgtatt aatccgctgt attcatactg
     5101 ttcgaatttt gaagataacg atgaaatatt ggttgctaga tcctggtatt cccattcgaa
     5161 ttgtggggta acaagattat attttaggtt tccgtcaatg attcttggtt ccgagccgtt
     5221 caagcttact ttgattttaa aatcctgagc cggaaaatca aataaacggg aaagatgccc
     5281 cacataatta tctaacgggt atccttgttt tttcgtcagc tcggttaaag ttatagttgt
     5341 tccgttttcg gtattgggtt taacagactc tttctgaaac tccggttgat aaatacgctc
     5401 acttttttta atctctgcat aatccaaagt aaaagtaacc cgttcgtttc cttggatagt
     5461 agagatttcg attttgttgc caagcctgaa taatgccaat ttaccaagac cttttttacc
     5521 cgttggaatt cttccgcacg gggaggcttg tttttcttcc cttctgttcc gaccgattct
     5581 caaataaaaa tcattgattt catcgaagct cattcctatg ccattatctt taataacgat
     5641 tttatgctcc ggtttatcga ataacctaac ttccacttcc gtagcacatg catcgtaggc
     5701 attcgctatc aactcggcaa tcgcaggaac agtgtgcgaa tacatcttta cacccaaatg
     5761 ttcgattacg gttggctcga aactcatttt caatttttct tcagacatgt tttctccttt
     5821 tctaacattc gatattatca acaattgccc tgcctatttt ttccgcatac atcggaggaa
     5881 cggcattacc aatcaaacgg gctatcttgt ccctactgcc cgctttaaag acatagttac
     5941 gagggaacga ttgcaatgtt gcaccttctc gcagggataa cgccctatct tcttcaggat
     6001 gggcaaaccg cccgttggaa atgctaaaga atttggtcgt tatggtaggt gcgggcttat
     6061 cccaccatag cctaccgaac gtgtctttaa aactattgtc ttttccgata aaacattcca
     6121 gctgtaattc gggaatatgc gcaaaagcca aacggtttcc tccgttttta ggtatcaaag
     6181 ccaatcgttt caaattgata tcggataatc ccgcacaact atgcataaaa tccgtctcgt
     6241 cttgatgtcc tgccataatt ttgggaaagc cgttttccat tcccaaaaca tcgcgtaccg
     6301 taagccgttt gcccgaatac ttgactggtt ccagcttttt ttggttattc tgtttgcaat
     6361 taacgtaaat cttttacggc tttggggaat tccgtaatca gccgtattgt gaataccgaa
     6421 atggacggta aatccttttt cttccaataa ctttatgaaa ttatcaagcc cgctttcttt
     6481 cattctgctc agaattcccg gtacgttctc tacaacaaca tatccgggat tgaaatattc
     6541 tacaaaccgc tgaaactcca aaagcaaact cttggatttt tccgattttc ttttgtctgt
     6601 ttgaataaca ctccaatatt gacaaggact acaacctata agaatcaaat catcatcatt
     6661 cttttttaag ccgagttctt tttccaaggt ttcaggctgc aattcaaaaa tatcagcctg
     6721 tataaatttg gcatttatat tggcttcata tgtttctttg cagctaagtt cgtaatctat
     6781 cccggctaaa acctgaatac cggcggatta cattccatag ctcattccgc ctccgccaca
     6841 gaaaaaatca accgccttta acttactgtt tttcatttct atttaaataa ttagac
//
LOCUS       DBJGGI010000087         6887 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.087, whole genome shotgun sequence.
ACCESSION   DBJGGI010000087 DBJGGI010000000
VERSION     DBJGGI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.087"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(163..639)
                     /locus_tag="ACSIGJ_001892"
     CDS             complement(163..639)
                     /locus_tag="ACSIGJ_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6948 domain-containing protein"
                     /protein_id="HHQ1330699.1"
                     /translation="MEANKFEVKSLSDLIKVFAGIAADFEAAMGVKRADISTEFDEPQ
                     HEPQPPVTVAEQKGINDFAIGKEVIIRTYSAGVWFGVLKQKAGNEVILTKARRMYSWW
                     AKESISLSGVARHGIRQDGSQICGELDSVWLEAIEIIPVTGGAAESIRTALEVAQS"
     gene            complement(672..872)
                     /locus_tag="ACSIGJ_001893"
     CDS             complement(672..872)
                     /locus_tag="ACSIGJ_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330700.1"
                     /translation="MNELISRINRFGARAKDGQSLLLKVGEICRDAAATWTTRKSESI
                     NHTAFTFTVKKDGLKEKVMIVL"
     gene            complement(1070..1483)
                     /locus_tag="ACSIGJ_001894"
     CDS             complement(1070..1483)
                     /locus_tag="ACSIGJ_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330701.1"
                     /translation="MIHLNVHYCDEIIRHAENDKYSLIGIFPDICHIPTPQAILGRLC
                     LSVSFSAEGMDIQTMKTGQIFLEIVRNDDVISALEIPSYDGSDTEENVSFMLHQTISG
                     LPVSDNDRIYVRMTTHNHILSESRPLSFSWLPYHS"
     gene            complement(1480..1941)
                     /locus_tag="ACSIGJ_001895"
     CDS             complement(1480..1941)
                     /locus_tag="ACSIGJ_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHQ1330702.1"
                     /translation="MGATLTVAVAACTVSTPTSAIPVTHIKCLRINGQIKCVKPISPN
                     TTPAAEHIEHVRKNPRRKAAMDRAAARIADKIALKAGGETFVSLRMKKGFTQSELATA
                     AGLPQPYLSRIENSKQSLQDKTVQKLANALGVSPLEVRAAFERRYEYMEQA"
     gene            complement(1958..2395)
                     /locus_tag="ACSIGJ_001896"
     CDS             complement(1958..2395)
                     /locus_tag="ACSIGJ_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330703.1"
                     /translation="MELTVHFNAEQDLDRLFEKDEEAVGYLENVIAMIQADSAIFDGL
                     YKNRYFREYGEPIGPIDLEVKPILSLWGKDIKVLRVRFDSEEAAGYRIIYAPCHEKQP
                     NGTYIRRIDILAVVNKKTDEFDYQAEHPITKRIIKDYEELYSN"
     gene            complement(2510..3226)
                     /locus_tag="ACSIGJ_001897"
     CDS             complement(2510..3226)
                     /locus_tag="ACSIGJ_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HHQ1330704.1"
                     /translation="MPFIIDYQCLCAINSEIKMFSGEQLGQAISEAIKRKNVSQKEVA
                     DHFGVKQPSVSGWIKNGRIDKKHLDKLIDYFSDVVTPSHFGIETFRVLKSNEQSSIRF
                     PRLNAEATCGAGTINDHYIEVVDYVTVAAAWAREKLGGNLNKIQVITARGDSMEPTIE
                     NGDVMFVDTAVEAFDGDGLYLLWYIDGLKAKRLQSTVGGGLMIISDNSSYRTETVRGE
                     DLNAVRIIGRIRGAWRLSQF"
     gene            3301..3531
                     /locus_tag="ACSIGJ_001898"
     CDS             3301..3531
                     /locus_tag="ACSIGJ_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HHQ1330705.1"
                     /translation="MSIQKAVDYFGNESRLARAIGVKQPTVWAWNKKGTPPPIIRCVQ
                     IEKLTGGAVNRKDLRPDDWHLIWPELAGDQPK"
     gene            3611..3766
                     /locus_tag="ACSIGJ_001899"
     CDS             3611..3766
                     /locus_tag="ACSIGJ_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330706.1"
                     /translation="MKKQDKNRLSKKDRRLIKKAMLKAAAKGCDEVYRIAPGLKDGFE
                     LLGKQPD"
     gene            complement(3743..3931)
                     /locus_tag="ACSIGJ_001900"
     CDS             complement(3743..3931)
                     /locus_tag="ACSIGJ_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330707.1"
                     /translation="MPSDLSFILAKELIRSGSIRLSGSTAKGQAGELAVFIRTLHQKP
                     EESEPNTDNEYLIGLLSK"
     gene            4104..4331
                     /locus_tag="ACSIGJ_001901"
     CDS             4104..4331
                     /locus_tag="ACSIGJ_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHQ1330708.1"
                     /translation="MNQKQTQCKQIVDYIRNKGCITSLEAYQNLKVTQLAARITDLEG
                     RGFVFAKPKYKVGNCKNPVAHYSIAKSGIEP"
     gene            4328..4465
                     /locus_tag="ACSIGJ_001902"
     CDS             4328..4465
                     /locus_tag="ACSIGJ_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330709.1"
                     /translation="MSREQRIRGARLPVSAYMQAEGAGKAREALDGRVEKVKGVDDEA
                     V"
     gene            5010..5513
                     /locus_tag="ACSIGJ_001903"
     CDS             5010..5513
                     /locus_tag="ACSIGJ_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330710.1"
                     /translation="MNADAFVSADAETCGRETGEPTSPKAESDSNGNGGLSGKPKNAN
                     VPRRRKTHGVPLQEIADLYNEVLGGRLPSVQVLNDTRKRAIANRWCEMLGTAAPNGKV
                     RFGDKETGLAWFAGFFRKVAMNPFWMGENQTGFAVGFDWIFKAGNFVKILEWHPPKTN
                     QAARGRA"
     gene            5510..6871
                     /locus_tag="ACSIGJ_001904"
     CDS             5510..6871
                     /locus_tag="ACSIGJ_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HHQ1330711.1"
                     /translation="MNRIEETEAVQSLASVGAEQNILGGILIEPTAIARCAILTPEKF
                     YQAQHRIIFRALLDMAAANEPIDIITLNDKLEARGEAENAGGLAYLIDLNQNTPSAKN
                     ISRYVGIVNDRFVERGLLKASAAIEKIAVSKDGGTVAEKLSKAADELAAAGKDAVKRE
                     TKTFGQTVEDLIGGLDKRLDGVRFGLPTGLMKLDGMTGGLPDGNLIVIAARPSMGKTV
                     LAENIARFALKQGKAVHFQSYEMSAVELARRGMAAECNIPMQNLKTGNLTQSDYANMP
                     IYVSQAKEWKFDVNCDLLNVDELCFLAKEKKLTTGLDLLVVDHLHIMPRAGRDEVAEL
                     GNISRRLKNLAAELNIPVVLVAQLNRGNTKQADKRPNMADIRGSGAIEQDANIIIMPH
                     RESYYDGNENPSIAELIIAKNRDGETGTVVCGWKGQFMKFEEEPDLAWQAPKHDEYDP
                     YSV"
BASE COUNT         1734 a         1587 c         1847 g         1719 t
ORIGIN      
        1 tgctgtagcc cttgccgttg ccgtagccgc tgccgctgcc gctaccgccg acgccgccgt
       61 agccgttgcc gtagccgttg ccgtagccgt tgccatagcc gctaccgttg ccgctaccgt
      121 tgccgttgcc gtagccgtgc ttcaatggtt gatctagata actcatgact gggcgacctc
      181 cagcgcggtg cggattgatt cagccgcgcc gcctgttact gggataatct caatcgcctc
      241 gagccatacg gaatcaagct cgccgcaaat ttggctgccg tcttgcctga tgccgtgtcg
      301 tgcgacacct gacaggctga ttgattcctt tgcccaccag ctgtacattc ggcgcgcttt
      361 tgtcagaatc acttcattgc ctgctttttg tttcaacaca ccaaaccaaa cgcctgccga
      421 ataagtgcgg atgatgactt ccttgccgat ggcaaagtcg ttgatacctt tttgctcggc
      481 aactgttacc ggcggttgcg gctcatgttg cggttcgtca aattcggttg aaatgtcggc
      541 gcgttttaca cccattgccg cttcgaaatc ggcagcaatg cctgcaaaga cttttataag
      601 gtctgacaaa cttttcactt caaatttatt tgcttccatt tttgtttcct ttcggggtgg
      661 ggttggtttc tttacaaaac aatcattacc ttctctttta agccgtcttt tttcactgta
      721 aaagtgaagg cggtgtgatt gatgctttcg ctttttctag tggtccatgt cgctgctgcg
      781 tcgcggcaga tttcaccaac tttcaataaa aggctttgcc cgtcctttgc cctcgcgcca
      841 aaccgattta ttctgctgat taattcgttc atcccgtttc ctttaagtgg ttgtttgttt
      901 cgatgggtgt attataacta ttacctatat ttaatcaata ggcaatagtt atattttcat
      961 ccatatatac ttattgaatt gatttttaga agaaaaaagt ttgaaaaaaa accgcccgaa
     1021 tggcggtgta tcatgaaaaa gaaaaccgcc ctagggcggt tcggtattgt tatgagtggt
     1081 aaggaagcca agaaaaagac aaaggacggc tctcggagag aatatggttg tgtgttgtca
     1141 ttcttacata aatgcggtcg ttgtctgaaa cgggtagtcc gctgattgtt tggtgtagca
     1201 tgaatgacac gttttcttct gtatcactgc cgtcatatga tggaatctca agtgcagaga
     1261 ttacatcatc gttgcggacg atttccagaa aaatttgacc ggttttcata gtctgtatat
     1321 ccatgccttc ggcggaaaat gaaacagaca ggcacaggcg gcctagaatg gcctgcggtg
     1381 tcggaatatg gcatatatcg gggaatatac ctatcaggct gtatttgtca ttttccgcat
     1441 ggcggataat ttcatcgcaa tagtggacat ttaagtgaat catgcctgct ccatatattc
     1501 gtaccgccgt tcgaacgctg cgcgaacttc aagcggggaa acgcctaatg cgtttgccaa
     1561 tttttgtaca gtcttatctt gcagggattg tttgctgttt tcgatgcggg acagataggg
     1621 ttgaggcaat cctgcggcgg ttgccaattc ggactgggta aacccctttt tcatccttaa
     1681 gctgacaaat gtttccccgc ctgctttgag ggctatttta tcggcaattc ttgctgccgc
     1741 cctgtccatc gctgctttgc ggcgcggatt tttccgcaca tgctcaatat gctctgcggc
     1801 aggggtagtg ttagggctta tcggtttgac gcatttaatc tgcccgttta tccgcaggca
     1861 ttttatgtgg gtgacgggga tggcggatgt aggtgttgat actgtgcagg cggccacggc
     1921 aacagtgagt gttgcaccca ttttgaacca tgtgccgtta gttggagtac agttcttcat
     1981 agtctttgat aatccgtttt gtgattgggt gttctgcctg atagtcaaat tcgtctgtct
     2041 tcttgtttac aacagccaat atatctatgc ggcggatata cgtgccgttt ggctgttttt
     2101 catggcatgg tgcgtaaatt attctgtatc cggcggcttc ttcgctgtca aatcggacgc
     2161 gtaaaacttt gatgtctttt ccccataatg acaatatggg tttcacttcc aagtcaatcg
     2221 ggcctatggg ttcgccatat tccctgaagt atctgttttt gtataagccg tcaaaaatag
     2281 cagagtccgc ctgaatcatc gcaatgacat tctcgagata accgaccgct tcttcgtctt
     2341 tttcaaagag gcggtctaaa tcctgctcgg cgttaaagtg gacggtcagc tccatgagtg
     2401 ccctttttat atatatatat tatatctttt tagttatacc gcgcaatatg cggtaataaa
     2461 tcagttgttc aagtatttta atccggcacg ctccaccaga agacgcggtc tagaactggc
     2521 tcaaacgcca tgcgccgcgt atgcgtccga tgatgcgtac ggcgtttaaa tcttcgccgc
     2581 gcacggtttc ggttcggtat gagctgttgt cgctgatgat catcaggccg ccgccgacgg
     2641 tggattgcag ccgcttggcc ttaaggccgt ctatatacca aagcaggtag aggccgtcgc
     2701 catcgaaggc ttcgacggcg gtatcaacga acattacgtc gccgttttcg atggtgggct
     2761 ccatgctgtc gccacgggct gtaatgactt ggattttgtt gaggtttccg cccagtttct
     2821 cccgcgccca tgcggcagcg acggttacat aatccacaac ctcgatatag tggtcgttaa
     2881 tcgtgcctgc gccgcaggtc gcttcggcat ttaagcgggg gaaacgtatg ctactttgtt
     2941 cattcgattt taagactctg aatgtttcaa tgccgaaatg gcttggcgtt actacgtctg
     3001 agaaataatc aattaattta tctagatgtt ttttatctat tcgtccattt tttatccaac
     3061 ctgaaacgct tggctgtttc accccaaaat gatcggcaac ctccttttga ctgacatttt
     3121 ttctcttaat cgcttctgat attgcttgtc ctaactgttc gcctgaaaac attttgatct
     3181 ccgaattaat tgcgcataag cattggtaat cgataataaa aggcataacc tataaaaggc
     3241 aatagttgta tttaatataa gtattagcta taatgaagct atttaattga gtaactggct
     3301 atgagtatcc aaaaagcagt tgattatttt ggtaatgaat cccgacttgc acgggcgatc
     3361 ggagttaaac aaccgacggt gtgggcttgg aataaaaaag gaacgccgcc cccgatcatt
     3421 cggtgcgtgc agattgaaaa attaaccgga ggcgcagtga atcgaaaaga cttacgtcct
     3481 gatgactggc atctaatctg gccggaattg gcaggcgacc aacccaaata aaaagcccgt
     3541 cgggtcagat gttaattgat ataaccgaat tgaagcggaa gtcatccgca atttaccgga
     3601 aaggaaaaaa atgaagaagc aggataaaaa ccgcctgtcg aagaaagaca gacggctgat
     3661 taaaaaggcg atgctgaaag ccgccgccaa aggctgcgat gaggtttaca gaatcgcgcc
     3721 gggtttgaaa gacggctttg aattacttgg aaagcagccc gattaaatat tcgttatcgg
     3781 tatttggctc cgattcttcg ggtttttgat gaagtgttcg gatgaatacc gccaattctc
     3841 cggcttgtcc tttggccgta ctgccgctta agcggataga accgctgcgg ataagctctt
     3901 tggcgagtat gaaggataag tcggacggca tttttttact ccgtcggccg ttgtgtggaa
     3961 acccggttgt aacggggtga cggcaaatcg gaaagacggt tgaccgcccg gacagacggg
     4021 cggccgataa agaaaaaccc gcacggggcg ggtaatcccc ctgaattgca gggaagcggt
     4081 tcaggtaacg gcgaaaggcg attatgaatc aaaaacaaac gcaatgcaaa caaattgtcg
     4141 attacatccg taacaaggga tgcatcacat cccttgaggc ttatcagaac ctgaaggtga
     4201 cgcagcttgc ggcacggata accgacttgg aaggcagggg cttcgtgttt gccaagccga
     4261 aatacaaggt cggtaactgt aaaaatcccg ttgcccatta ctcaatcgca aagtcaggaa
     4321 ttgaaccatg agccgggaac aaagaatacg cggagcccgc ttgccggtgt cggcttatat
     4381 gcaggcggaa ggcgcgggca aggcacggga agcgttggac gggcgggtcg aaaaagtgaa
     4441 aggggttgat gatgaagccg tctgaaagtt tgagagcggc aggcaggcca atcgcgtatt
     4501 acccgaaatt ggcaaagcct ttgggcggtg taaatgcggc aatattattc gggcatttct
     4561 tctactggaa cgataagacg cagtatgaat caggcattta ccgaacagcg gaagaaattg
     4621 aaattgaaac cgggctgtcc gttcaggaac aaagaacggc acgggcaaag ctgagggaac
     4681 gcggcgtatt gattgagact gaaaaacgaa ttgaacaccg catttactac aaactgaatt
     4741 tagacgcttt tgatgatttg atgttgcaac attcgggggg tggggaatca acagccccga
     4801 aatgcaatat caacagcccc gaacttcaaa atcaacattc ggggggtggg gaatcaacag
     4861 ccccgaaatg caatatcaac agccccgaac ttcaaaatca acattcgggg agtgaggaat
     4921 caacagccgt tataagaaca gaagatttaa cagaagattt agcagtatat acccccttac
     4981 ccccaaacgc cggaaacggc aaaggcggtt tgaacgctga cgcgtttgtt tccgctgacg
     5041 cggaaacgtg cgggcgggaa accggcgaac cgacttcgcc gaaggccgaa agcgacagta
     5101 acggcaacgg cggcctttcg ggaaaaccga aaaatgcgaa tgttccgcgc cgccgcaaaa
     5161 cccacggcgt accgcttcag gaaatcgccg atttgtacaa cgaagttttg ggcggccggt
     5221 tgccaagcgt ccaagtgctg aacgacacgc gcaaacgggc gattgcaaac cgctggtgcg
     5281 agatgctggg aacggcggcg ccaaacggca aggtgaggtt cggggacaag gaaaccggtt
     5341 tggcctggtt tgccggtttc ttccggaaag tggcgatgaa cccgttttgg atgggcgaaa
     5401 accaaacagg gtttgcggtc ggcttcgatt ggattttcaa ggcgggcaat ttcgtcaaaa
     5461 tccttgaatg gcatccgcct aaaacgaacc aggcggcaag gggaagggca tgaaccgaat
     5521 cgaggaaacg gaagccgtcc aatcactggc cagcgtaggg gcggaacaga acattttggg
     5581 cggcatcttg attgaaccga cggcgattgc gcggtgcgca atcctgaccc ctgaaaagtt
     5641 ttaccaggcg caacacagga ttattttccg cgctctgctg gatatggcgg cggcaaacga
     5701 gcctatcgac atcatcacgc tgaacgacaa gctggaagcg cgcggcgagg cggaaaacgc
     5761 gggtggcctg gcttacttga tagacctgaa ccaaaacacc ccaagcgcga agaatatcag
     5821 ccggtacgtt gggattgtga acgacaggtt tgtcgagcgc ggcttgctga aggcttcggc
     5881 ggcgattgaa aaaatcgcgg tttccaaaga cggcgggacg gtcgcagaaa agctgtctaa
     5941 ggcggccgac gaattggcgg cagccggcaa agacgcggta aagcgtgaaa ccaagacatt
     6001 cggccagacc gttgaggatt tgattggcgg tttggacaaa aggcttgacg gcgtgcgttt
     6061 cggattgcct accggcttga tgaagcttga cgggatgacc ggcggtttgc cggatggaaa
     6121 cctgattgtg attgcggcgc gtccgtctat gggtaaaacg gttttggcgg aaaacattgc
     6181 gcgattcgcg ctgaagcagg gcaaggcagt tcatttccaa agctacgaaa tgagcgcggt
     6241 agagctggca aggcgcggca tggcggctga gtgcaatatc cccatgcaga acctgaaaac
     6301 cggcaatctg acgcaaagcg attacgccaa tatgccgatt tacgtcagcc aagcgaaaga
     6361 gtggaagttt gacgtgaact gcgacctgct caacgttgac gagctttgct ttttggctaa
     6421 ggagaaaaaa ctcactaccg gcttggattt gttggttgtc gatcaccttc acatcatgcc
     6481 aagggcaggg agggacgagg tggcggagtt ggggaatata tcgcgccgtt tgaaaaactt
     6541 ggcggcagag ctgaatatcc ccgtcgtctt ggttgcccag ttgaacaggg gaaacacaaa
     6601 gcaggcagac aaacgcccga acatggcaga cattcgcggc agcggcgcga ttgagcaaga
     6661 cgcaaacatc atcatcatgc cgcaccgcga aagctactac gacggaaacg agaatccgag
     6721 cattgccgag ctgattatcg ccaagaaccg ggacggcgaa acgggaacgg tggtttgcgg
     6781 ctggaaaggg caatttatga agttcgagga agagcctgat ttggcatggc aagcccccaa
     6841 acatgatgaa tatgaccctt acagtgtcta gtgcgggagg ccggtaa
//
LOCUS       DBJGGI010000088         6711 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.088, whole genome shotgun sequence.
ACCESSION   DBJGGI010000088 DBJGGI010000000
VERSION     DBJGGI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.088"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(387..1604)
                     /gene="nqrF"
                     /locus_tag="ACSIGJ_001905"
     CDS             complement(387..1604)
                     /gene="nqrF"
                     /locus_tag="ACSIGJ_001905"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HHQ1330712.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGSKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            complement(1618..2211)
                     /gene="nqrE"
                     /locus_tag="ACSIGJ_001906"
     CDS             complement(1618..2211)
                     /gene="nqrE"
                     /locus_tag="ACSIGJ_001906"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HHQ1330713.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            complement(2215..2841)
                     /locus_tag="ACSIGJ_001907"
     CDS             complement(2215..2841)
                     /locus_tag="ACSIGJ_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HHQ1330714.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTIFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            complement(2841..3617)
                     /locus_tag="ACSIGJ_001908"
     CDS             complement(2841..3617)
                     /locus_tag="ACSIGJ_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HHQ1330715.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVKDAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKVSQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            complement(3610..4842)
                     /locus_tag="ACSIGJ_001909"
     CDS             complement(3610..4842)
                     /locus_tag="ACSIGJ_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HHQ1330716.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYVLYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSPEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFASVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVANPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            complement(4845..6188)
                     /locus_tag="ACSIGJ_001910"
     CDS             complement(4845..6188)
                     /locus_tag="ACSIGJ_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HHQ1330717.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYVGMRPSMK
                     IKEGEAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYVPEALAKLSSEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFKRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFVTGRLNTERVVALGGLQVNKPRLL
                     RTVLGAKVSQLTAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRGKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            6426..>6711
                     /locus_tag="ACSIGJ_001911"
     CDS             6426..>6711
                     /locus_tag="ACSIGJ_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HHQ1330718.1"
                     /translation="MKITHCKLKKEVQKEPLRSFVPEVTARSAADILGIHPDSAALFY
                     RKIRTVANHRLALAADEVFEGPAGPGGSCFGGRRKGRRGRGAVGKAVVFGI"
BASE COUNT         1598 a         1709 c         1794 g         1610 t
ORIGIN      
        1 aaccggtttg tcagggttta atggtcagcg cgtcctgcgt tgccatttgt tgtagtagta
       61 ttcgtaatct tcatcacgac cgcccgattc aatccaatca tcgtattcgg ggctgttttc
      121 aaagctgtca gacattatcc gtacctcgca aaatgggttt atcgatactt ttttagttat
      181 ggaatcaacc ataactaaaa agaagtatac ttctttgctt ttccgccaaa atatttaacc
      241 gcacatccgc cgcaggatac aaagatagga aaacaacgga tatttatatg ggcattggcg
      301 gatgccgtct gaaggatttc agacggtatc gggataaaaa aaggaagaaa ccgaagtttc
      361 ttccttttgt gtttgaagcc aaatatttag ccgccgaaat cgtccaagag gatgttttcg
      421 tcttccacgc ccaagtcttt gagcattttg atgacggact ggttcataat cggagggcca
      481 cacatataga attcgcagtc ttccggtgct tcatggtttt tcaggtggtt ttcgtaaact
      541 acgttgtgaa taaagcctgt gtaaccatcc cagttgtctt caggcaatgg gtcggacagg
      601 gcaacgtgcc atgtgaagtt cgggaattct gctgcaagtt ggtcgaagtc ttcgacatag
      661 aacatctcgc gtttagaacg tgcgccgtac cagaaggtga ttttgcgttt ggagttcaaa
      721 cgtttcagct ggtcgaaaat gtgggaacgc atcggagcca tacccgcacc gccgccgatg
      781 aataccattt cggcatcggt gtctttggcg aaaaattcgc cgaacgggcc ggaaatcgta
      841 actttgtcgc cgggtttgag cgaccagatg taggaagaca tttgtcccgg aggcgcatcg
      901 ggtacgcgcg ggggcggcgt ggcgatacgc acgttcagca taatgatgcc tttttcttca
      961 gggtatgaag ccatagagta ggcacgcaaa atcggctcgt ccactttgga aacgtattgc
     1021 cacagattgt atttgtccca gtcttcgtga tattccttgg gaatgtcgaa gtctttgtag
     1081 gcaacagtgt gaggaggagc ttcaatttga atgtagccgc cggcgcggaa ggggacttct
     1141 tcgccttcgg gaatggcaag cttgagttct ttaatgaacg tggctttgtt gtcgttggaa
     1201 atgacggtgc attcccattt tttcacgccg aacacttctt cggggacttc gatgtccatg
     1261 tcggttttga cgttgacctg acaagacaga cggcagcctt cgcgtgcttc gcgtttgctg
     1321 atgtgggaca gctcggtcgg caggatgtcg ccgccgccgc tttttacgac gacgcggcat
     1381 tgtccgcacg aaccgccccc gccgcaggcg gaggggataa agatgccttc gttggcaagc
     1441 gcgcccaaga gtttgctgcc ggcgggcatc gtcagctctt tttcgccgtt gactttgatg
     1501 gtgatgtcgc cttcgctgac cagtttggat ttggcaaaca gaatcatcag tgccaaaacc
     1561 aaaacgatga cggtaaacat cacgatacct aaaataatct ccataccggt ccctttctta
     1621 taactggatg ccggagaacg acataaacgc catcgccatc aggccggcgg cgataaaggt
     1681 aatgcccaag cctttgaggc ctttgggagc gtccgaatat ttcatttttt cggtaatgcc
     1741 cgccaaagcg acaatcgcca acatccagcc taagcccgcg ccgaaaccgt acacgacgga
     1801 ctcgccgaag ttgtattcgc gttgcgccat aaacgatacg gcgccgaaaa tcgcgcagtt
     1861 cacggtaatc agcggcaggt agatgcccag cgcgttatag agggcgggga cgaatttgtc
     1921 caagaacatt tccaaaatct gcaccaaagc ggcaatcacg ccgatgaagg tgatgaattt
     1981 caaaaaggtc aaatccacgc cttcgacaat cgcgccgtct ttgagcagcg agtaaacgag
     2041 ttggttgaca gggacggaca gcccgagtac gaaaattacc gccacaccca aaccgaatgc
     2101 ggtggatact tttttggata ccgccaaaaa cgtgcacata cccaaaaaga aggatagcgc
     2161 catattttca atgaagacgg atttgatgaa gaggctcaaa tagtgttcca tagcttattc
     2221 ctccgcctgt tcgggtttcc aggtacgcag tccccaaatc aaaaagccga tgatgaagaa
     2281 cgcgctgggg gcgagcagga acaagccgtt ggtctgatac cagccgccgt cctgcacggt
     2341 ttggaaaatg gtgtagccca agagtttgcc cgagccaatc agttcgcgga cggtggcgac
     2401 gacaagcagc atcatcccgt agcccgcgcc gttgccgatg ccgtcgatca ggctttccag
     2461 cggcggctct ttcatcgcaa atgcttcggc gcggcccatc acgatacagt tggtaataat
     2521 cagaccgacg aatacggaaa gctgtttgga caattcgtag gcaaatgcct gcaagagttg
     2581 gtcgaccagc gtaaccagcg acgcgataat cgccatttgc acgataatac ggatgctgtt
     2641 ggggatgtag ttgcgtacca gcgaaatgaa gaagctggaa aaaccggtta ccaaagctac
     2701 ggaaataccc atcacgatgg ccgtctgaag tttggtggta accgccaaag ccgaacaaat
     2761 acccaaaacc tgcaaggcaa tcgggttgtt gtcgataaag ggtgaaaaca tcaaatgttt
     2821 caagcgtttc atatcagcca ttattgcgct cctgctgatt tcaatttgtt caggtagggg
     2881 atatagccgt tttcgccgaa ccagtaggcg aacgaacctt gcacgccttt ggatgtcagc
     2941 gatgcgccgg agagggcatc gacgccgtgt tctttgtccg aacccgcgcc tttgccgacg
     3001 tgcagggcga gtttgccttg tccgtcaaac agttttttgc cgacgaattt ttgctgccac
     3061 aacggattgc cgatttcgcc gcccaagccc ggggtttcgc cttgttcgta gtaggtaatg
     3121 ccgttgatgg tgttgccgtc cggttgaatg gcgacaaagc cgtacatcac cgaccacaaa
     3181 ccgttgccgt gcatcggcag gatgatttgg ctgactttgc cgtcttcgcc ttttaccaag
     3241 taaacctcag tgtatttggc acggcttttg atgcctgcca aatcgtcttc aggtttgatg
     3301 cggatgcttt gcgcggggtc tttgcctgcg atgcgcgcgc tgaagtcttt cggcgcgtct
     3361 ttcacatatt cgccggtcgc caaatcgacc acgcgttgct cgatacgctc ggcaaaggtt
     3421 ttaccgatgt cggtgtcctt atccatcaaa ccggccacgc tcaaaatata gccttgtttg
     3481 tcttggagtt tttgtttttc ttggatgggt ttcaagccga cgaccgcacc ggcaacgatg
     3541 accgagcaaa tcaggctgac cgccaacacg acaatcagcg tgccgctgaa gctgtcttta
     3601 tcgaatttct tagccattgc tgcgcgcctt tctgcgtttg atgttcgctt gtgcgacgaa
     3661 atagtcgaaa atcggggcaa acaggttggc aaacagaatc gccaacatca tgccttcggg
     3721 gtaagccgga ttggccacgc ggattaacac gcacattacg ccgataagcg cgccgtacca
     3781 ccatttgccg acattggtaa aggaagcgga aacggggtcg gtcgccataa acagcatacc
     3841 gatggcgaag ccgccgacca ccaagtgcca gtaccaaggc atagcaaaca tagcgttggt
     3901 gtcagaaccg atgaagttaa acagcgaaga catcgcaatc ataccgatca tcacgccggc
     3961 aataatgcgc caagaagcga tgcgggcaaa cacgataaac gcgccgccga ttaagagtgc
     4021 caaagtggag acttcgccga tggagccggg cagtttgccg ataaacgcgt ccatccaagt
     4081 gatggtttga ccggttacgg cgtttttcag gccgtctgca ccgtgtgccg cccattgcgc
     4141 cagcgcggtt gcgccggaat agccgtcaac cgccgtccaa accgcatcgc cgctcaagtt
     4201 ggcggggtag gcgaagaaca ggaaggcgcg gcctgccagc gcagggttca tgaagttttt
     4261 acctgtaccg ccgaatactt ctttcgcaac cacaacgccg aaagaaatac ccaaagccgc
     4321 ctgccacagc ggcagcgtgg gcggaacgat taaggcaaac agaatcgaag taacgaagaa
     4381 accttcgttg atttcgtgtt tgcgtacgga tgcgaacaag acttcccaga agccgcccac
     4441 aataaatacg gtcgcgtaaa tcggcaggaa gtaaatcgcg ccgaacagca ttttgcccaa
     4501 cacgcccgct tcaggcgaca tattgatgcc caaagcgttg gcaagggcgt aatgccagtc
     4561 gttggcgatg ctttgttgca gcaaatcggg cgttaaggca ccgaatgcct gtgcgccgac
     4621 gttgtacatg ccgtaaaaca tggcggggaa caaagccagc cacaccaaaa tcatcatgcg
     4681 tttggagtcg agcgcgtcgc ggacgtgcgc cgctttgcgc gttaccgcgc cggatgtata
     4741 gaaaatcgtc gccgcagctt catagaggac ataccatttt tcatgtttgc cgcccggcag
     4801 gaagtgcggt tcgatttttt ccagaaaatg ttttaagccc ataatcagcc ttccttctca
     4861 atggtttcca gcactttgcg caacagcggg ccgtattcgt atttgcccgg gcagacgaag
     4921 ctgcacaaag cgaggtcttc ttcgtccaat tccaagcaac ccaaagcctg cgcgctgtcg
     4981 gtatcgccga cgattaaatc gcgcaaaagc aaggtaggca ggatgtccaa cggcattacg
     5041 cgctcataag tgccgatcgg taccatggcg cggtcgccgc cgttgacggc tgtcgtgaac
     5101 ttgaagagtt tgttttttag gaaatggccg agagtggtgc gcgtgatgga gtatttgtcc
     5161 ggctgcggcg caacccagcc gaacagctct ttgccgcggc cttcttcgat aacggaaatc
     5221 tgattgtggt agcgtcccaa ataatcatgc gcgccttgtg caatcgcacc gttcaatacc
     5281 gaaccggaaa tcacgcggtt gtccgcgtca accaattcgc cggcggtaag ttgagacacc
     5341 ttcgcaccca aaacggtacg caagaggcgc ggtttgttga cttgcaggcc gcccaaggca
     5401 accacgcgct cggtattcag acggcctgtt acgaacaaac gtccgatagc aatcacgtct
     5461 tgataattga tggtccacac ggttttattc gcgccgactg gctcgatgaa atgaatgtgc
     5521 gtgccactca agccggcagg atgcgggccg ccaaattcat gtgtttcgat attggcagca
     5581 ttttcagacg gcacgtctgc gcctgctgct ttacacacat ggattttacg ttcggtcagg
     5641 cggctcaata ccaacaggcc gcgtttgaag tcttcggcgg cttctttgat gatgaccgta
     5701 gggtcggcag ccagcggatt ggtgtccatc gcattgacga agatggcgaa cggctcggca
     5761 tctacggcag ggattttgct gaacggacgg gtgcgaagcg cagtccataa gcctgattga
     5821 atcaggttgc ggcgcacttc ttcgctgctc aattttgcca gcgcttcagg tacgtagcgt
     5881 tcgaactcga tttcgtcgtt gccttcaacg gcaatcacga ctgactgaag tacgcgcttt
     5941 tcgccacggt gaatagcggc gattttgcct gaagccggcg cagtaaatac tacgcccgga
     6001 ttctttttgt cttcaaacag cacttggcct tttttgacgg cttcaccttc cttgattttc
     6061 atcgaggggc gcatgccgac atattcttcg ccaagcaacg cgacttcggt aatggccggg
     6121 ccgtcataaa tgacttgctc cggtctgccc gcgatgggca gatttagacc ttttttgatt
     6181 ttaatcatag atttgcatta cttgtgatgg ttaaggtaaa aacggcgtgt ttttgatacc
     6241 gtgtcgcgtg gcatcaaaag cattgaataa attaatgtag caaagtgtta gattttatca
     6301 ggaattgtac ctgtttgtca gatttgccgc ttttttcctt gcggaagccg tttttaattg
     6361 tcggaagggg gatattgatt tgataagggt ctgtaccaga ttagcagata tgttaccctc
     6421 gaaatatgaa gataacacac tgcaaattaa agaaagaagt acaaaaagaa ccgctccgtt
     6481 cttttgtacc ggaagttacc gcccgttctg ccgccgatat tttgggtatc catcccgatt
     6541 cggcggcact gttttaccgt aaaatccgca cggttgccaa ccatcgtttg gccttggctg
     6601 ccgatgaggt tttcgagggc cccgccgggc cgggcggaag ctgtttcggc gggcggcgta
     6661 aaggcagacg cggtcgcggc gcggtaggaa aagcggttgt cttcggcatt c
//
LOCUS       DBJGGI010000089         6413 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.089, whole genome shotgun sequence.
ACCESSION   DBJGGI010000089 DBJGGI010000000
VERSION     DBJGGI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.089"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            236..997
                     /locus_tag="ACSIGJ_001912"
     CDS             236..997
                     /locus_tag="ACSIGJ_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330719.1"
                     /translation="MEMQLDKQTLTVVVRNSEPVELSVFAQSMMSLADDYESMCGSAG
                     SHARLYIKEIRQGSIIAELAPLLPLAGTLFEGAGQILAYAKNFIEITDWLMGKGKEPA
                     GVTDSQIRNIANIMQPAASDKNGSIEINVNDNNGSVVNNITYNYFAANTVQNQARRIL
                     GERAEASESGDYGQMVMYFVQAAPTKETNQAVIEGIYSRPVKILIPEHIKREMFAEPY
                     PFEKYYIVDVSVQTARGKPRLYKVTGYHGVVDGDD"
     gene            complement(1096..1497)
                     /locus_tag="ACSIGJ_001913"
     CDS             complement(1096..1497)
                     /locus_tag="ACSIGJ_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HHQ1330720.1"
                     /translation="MFIPAALHKDEHSAYGVTIPDLPGCFSCGDTVEEAVANARSAAY
                     MHIDGMIEDGGFKNLAVSSIADLSQEPDYHGATWVMIEIDPAKISRQQIRFNVSWPQY
                     LLDRVDEYTSANHETRSGFLAKAALLTMNQA"
     gene            complement(1599..1781)
                     /locus_tag="ACSIGJ_001914"
     CDS             complement(1599..1781)
                     /locus_tag="ACSIGJ_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HHQ1330721.1"
                     /translation="MNSLDVIALLKQDGWYKVAQSGSHSQYKHPTKKGRVTVPHPKKD
                     LPTGTVKNIYKQAGLK"
     gene            complement(1951..2769)
                     /locus_tag="ACSIGJ_001915"
     CDS             complement(1951..2769)
                     /locus_tag="ACSIGJ_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HHQ1330722.1"
                     /translation="MNQYAMRFAVIRFMPYVQTREFANIGIIITHPKSGYFDFKIEQR
                     YSRLSRFFRRFDSSVYKAATRAFAEELQRIGKLAAYSAPDQIRAMLDHLTRPREALIM
                     AAQPGVTLAPDRGQELNRLFDYFVARSFAKSQPEAELTRQIQAMLKPLQTAYPFKEST
                     IGDPSGFHASIPLVQKAENGEIRKIIKPIYFGQKDPADIYYKSDKWIAGIKRLRRGGY
                     IDRSEILFAYEPPERPDKAQEKALLDVLGDLEEQGIRLADNRSEGKIIRNFACG"
     gene            complement(2766..3464)
                     /locus_tag="ACSIGJ_001916"
     CDS             complement(2766..3464)
                     /locus_tag="ACSIGJ_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HHQ1330723.1"
                     /translation="MDRAEQGVTSPFICMAENGLEYFVKGLHATRASQINEWIGGNMA
                     QALGLPVAPFELLEVGEELYEELPTKMKEIGKGICFGSLAQKGCALLEPADIPRIDTV
                     MQRQIAAFDWFVRNEDRTIGNPNLLYRNCNNSLIVIDHNCAFDTGFNPNNFLQNHIFS
                     SAFRQILEDWVLREEMELWLKSALPAYRKACDNLPPEWAWANEERDLPAAYNRSRTDE
                     TVRRIDNGTLWSIS"
     gene            complement(3703..4401)
                     /locus_tag="ACSIGJ_001917"
     CDS             complement(3703..4401)
                     /locus_tag="ACSIGJ_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HHQ1330724.1"
                     /translation="MNRVDRIKNLIADRFNGNQAEFSRAINKAPAQINQWLNGYRNIG
                     DGVAAQIETALGLPRGWVDGKDEPNIPIDSIKSNATVIGTVDAWDSKTPLSDDDCEVP
                     FYKDVCLSAGNGFSDEIEDYNGYKLRFSKSTLRRHGISPDDVVCVSADGDSMEPVFPD
                     GATLGINTADKMIKDGKIYAINHSGLLRTKILQKLPDNQVRIKSYNPEYKDETASLDS
                     LTVIGRVFWWSVLD"
     gene            complement(4441..5094)
                     /locus_tag="ACSIGJ_001918"
     CDS             complement(4441..5094)
                     /locus_tag="ACSIGJ_001918"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012920.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HHQ1330725.1"
                     /translation="MKNLVEEAGSVAELAKRAGYAQPSYLYQIINQTAIQNGKPKNIG
                     GAMARKLESAMNKAEGWLDMNHGETLPLPPEDTETIRIDHLDMAGACGGGIEADDWPS
                     PISSVEFPLDAVRRLFSGRDTSGLKIVGTRGDSMEPTIPETSAVLIDTKADTFAGDGI
                     YFFAYAGGLYTKRLQKTPAGLLALSDNPLYQSFPIDENDRFRIIGRYYGVISLKLAQ"
     gene            5292..5477
                     /locus_tag="ACSIGJ_001919"
     CDS             5292..5477
                     /locus_tag="ACSIGJ_001919"
                     /inference="COORDINATES: protein motif:HMM:NF027273.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HHQ1330726.1"
                     /translation="MTRLAAAINEKLSNLSSMRHGKKTVPPYKCRRIVEATNGEVTLK
                     DLRPDDWHEIWPELKED"
     gene            5479..5679
                     /locus_tag="ACSIGJ_001920"
     CDS             5479..5679
                     /locus_tag="ACSIGJ_001920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330727.1"
                     /translation="MEIVPVKWEDAFGCPEGWQMLDGVGRETSLVQSVGFVAAETETT
                     LTIVPHVGGLNRERGRLLERVY"
     gene            5876..>6413
                     /locus_tag="ACSIGJ_001921"
     CDS             5876..>6413
                     /locus_tag="ACSIGJ_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HHQ1330728.1"
                     /translation="MKYIPNSFQIANAVVDDFLCRMSGNAWKCYAVIVRKTTGWQKEI
                     DYISVSQFKNLTGIKTDVTVADALKELVELNLIASVKRHGQVTGYRINMPEPSPENGG
                     TPPPENGGTATPKNWGSTKHTTKPTNTKHSISASAAADAPLSAEPPETAPAAKAKKTG
                     RHETELSLLADYGITGQVA"
BASE COUNT         1555 a         1492 c         1693 g         1673 t
ORIGIN      
        1 ggttaattcg ttcatctcgt ttccttcaag ttgttgtttg tttcgatggg tgtatattat
       61 ctaatagata atcttaggtc aataagttag ataagttttt tagataattt ttattatcta
      121 tttgaatttg aaaagaatta aattcttctg tttgctcgta tcttttaggc aacaaaaaaa
      181 ccgcccgata tttcgggcgg cgtattaaaa tcagatgaaa ttaaggaggc ttattatgga
      241 aatgcaattg gacaaacaga cattgacagt ggttgtcaga aacagcgagc cggtggaatt
      301 gtcggtattt gcgcagtcta tgatgagcct tgccgatgat tacgaatcca tgtgcggcag
      361 cgcgggcagt catgcgcggc tgtatattaa ggaaatcagg cagggcagca ttattgccga
      421 gcttgcaccc ctcttgcctt tggcgggtac gctttttgaa ggcgcggggc agatattggc
      481 gtacgccaag aattttattg agattaccga ttggctgatg ggtaaaggca aagaacccgc
      541 aggggtaacg gacagccaaa tccgcaatat tgcaaatatc atgcagcctg ccgcatcgga
      601 taaaaacggg tcgattgaaa tcaacgtgaa cgacaacaac ggctccgtcg tcaataatat
      661 tacctacaac tatttcgcgg caaacacggt acaaaatcag gcaaggcgga tactgggcga
      721 acgggcggaa gcgtccgaat caggggatta cgggcagatg gtgatgtatt tcgtacaagc
      781 cgcgccgaca aaggaaacca atcaggcggt tattgaaggg atatacagcc gtccggtcaa
      841 aatcctgatt cccgaacaca tcaagcgcga aatgtttgca gagccttatc catttgaaaa
      901 atattatatt gttgacgtaa gcgtacagac ggcgcgcggc aagcccaggc tctacaaagt
      961 taccgggtat cacggggtag tcgacgggga cgattgaaaa ccgccttttc gggcggcttt
     1021 gtcggttttg tgttgttttc aggttcggcg gggcgtgaaa aaagcccgcg tgtgcgggcg
     1081 ggttgaacgg gctggctatg cctgattcat ggtgagcagg gcggctttgg ctaaaaaacc
     1141 gctacgggtt tcatgatttg cggatgtata ttcatccact ctatcaagaa ggtattgcgg
     1201 ccagctgacg ttgaatcgga tttgctgccg actgattttt gcagggtcga tttcaatcat
     1261 tacccatgtt gccccatgat aatcaggttc ttggctcaaa tcggcaatgc tgcttacggc
     1321 aaggtttttg aaccctccgt cctcaatcat accgtcaata tgcatatagg cggcagaacg
     1381 ggcgttggcg acggcttctt caacggtgtc gccgcaagag aaacagccgg gaaggtcggg
     1441 gatggttacg ccatatgccg aatgttcgtc tttgtgcagg gcggcaggga taaacatttg
     1501 atttccttta attatgttgt tcaggtttat gggcgcgtga gataacccgt cgattgtttg
     1561 attagaaggg gggcggcggg aaaccccgct gcctgccgtt acttcaaacc ggcttgctta
     1621 tagatatttt ttacagtacc tgtcggcaag tcttttttcg ggtgcggcac ggttacacgg
     1681 cctttttttg ttgggtgctt atattgcgaa tggctcccgg attgtgcaac tttataccaa
     1741 ccgtcttgtt tgagtagggc aataacgtct aggctattca attatcactc ctaatcttaa
     1801 tgcatggcac gcctgccaat gtgcgtaata atacacacta atttttattt tgtcaatttt
     1861 tatgtgtatt tgtgtgtaaa aatatctttc ggcagtagtt gcagggaagg gtgtaaaaag
     1921 cccgcataac gcgggcggtc ttgagggctg tcagccgcag gcaaagtttc tgatgatttt
     1981 gccttcgctt cggttatcgg caagccgtat gccctgttcc tccaggtcgc ccaatacgtc
     2041 aagcaatgcc ttttcttgtg ccttgtcggg acgctccggc ggttcgtagg caaacaatat
     2101 ttcagagcgg tcgatgtatc cgccgcgccg caaccgtttt atgccggcaa tccacttatc
     2161 agacttgtaa tagatgtcgg cagggtcttt ctgtccgaaa tagataggct tgatgatttt
     2221 ccgtatttcg ccgttttccg ccttttgcac caaggggatg gaggcgtgaa agcctgacgg
     2281 gtcgccgatt gtgctttctt tgaaggggta tgccgtctga agcggcttta acattgcctg
     2341 tatttgtcgg gtaagttctg cttcgggttg gcttttagca aagctgcggg cgacaaaata
     2401 atcaaacagg cggttcagtt cctgccccct gtcgggggcg agggttacgc ccggttgggc
     2461 ggccataatc agggcttcgc gcgggcgggt caaatggtcg agcatggcgc gtatctgatc
     2521 gggcgcggag tatgccgcca atttcccaat ccgctgtaat tcttctgcaa aggcacgggt
     2581 cgccgcttta tagacggacg aatcgaagcg gcggaaaaaa cggctcaatc tgctgtaacg
     2641 ctgttcgatt ttaaagtcga agtagccgct tttgggatgg gttatgatga tgccgatgtt
     2701 ggcaaattcg cgcgtttgga catagggcat aaaccgtatg acggcaaaac gcatggcata
     2761 ttggttcatg agatgctcca aagtgttccg ttatcaatgc ggcggacggt ttcgtcggtg
     2821 cggctgcggt tataggcggc gggcaggtcg cgctcttcgt ttgcccatgc ccactccggc
     2881 ggcagattat cacacgcttt tctgtaggct ggcaatgcgc ttttcagcca taattccatc
     2941 tcttcccgaa gcacccaatc ttccaaaatc tgtctaaatg ccgatgagaa aatatgattt
     3001 tgcagaaagt tatttggatt gaagccggtg tcgaaggcgc agttatgatc aataacaatc
     3061 aaggaattgt tgcaatttct atacaaaagg ttagggttgc cgattgttct gtcttcatta
     3121 cggacaaacc aatcgaaagc ggcgatttgc cgctgcatga ctgtatcgat tcgcgggatg
     3181 tccgcaggct ccagtaacgc acagcctttt tgcgccagtg agccgaaaca gatgcctttg
     3241 ccgatttctt tcatctttgt gggcaattct tcgtacagct cttctccgac ttccaacagc
     3301 tcaaacggag caacgggcaa gcccaaagcc tgcgccatgt ttccgcctat ccattcgttt
     3361 atttggcttg cacgggtggc gtgcagccct ttaacgaaat attccagacc attctccgcc
     3421 atacagatga acggcgaggt aacaccttgc tccgcacgat ccattattgt ctgtatctgc
     3481 aacatttctt ttctttatcc tatgtttcaa cacacaggac gacacataaa gcaccgccct
     3541 atgtgttgcc ctgattcgga aggggttacg cccctcccaa acagagtctg atcctgccgc
     3601 cctaaagggc ggggtttcaa ccgaaaagga aatacgatga aatttacact ttgtgtaaat
     3661 ctgtaaatcc gactttttga gatttccccc tagaggaatt tgtcaatcca gcacgctcca
     3721 ccaaaatacc ctaccaataa cggtcaggct gtctaaagag gcggtttcgt ctttgtattc
     3781 cgggttgtaa ctcttgatac ggacttgatt atcaggcagc ttttgcaaga tttttgtacg
     3841 taacagccca ctgtggttga tggcatagat tttgccgtct ttaatcattt tatcggcagt
     3901 attaataccc agtgtagccc cgtcggggaa taccggctcc atactgtctc cgtcggcaga
     3961 tacgcagaca acatcgtcag ggcttatgcc gtgccgtctt aaggttgatt ttgagaaacg
     4021 cagtttatag ccgttgtagt cttcaatttc atctgaaaag ccgttacccg ccgacaagca
     4081 aacgtcttta taaaatggaa cttcgcaatc atcatcggat aacggggttt tgctatccca
     4141 agcgtcaaca gtgccgataa cggtggcgtt tgatttgatg gaatcaatag gtatattcgg
     4201 ctcgtctttt ccatccaccc atccgcgcgg taatccaagc gcggtctcaa tttgggcggc
     4261 tacgccgtcc cctatatttc tgtatccgtt aagccattga tttatctgtg caggagcttt
     4321 gttaattgcc cgagaaaact cagcttgatt gccgttaaac ctgtcggcta tcagattttt
     4381 aattctatct acccggttca tttcatcacc ccttcaaata gatataaata taaggcatag
     4441 ctactgcgcg agcttcaggg aaatcacgcc gtagtatctg ccgatgatgc ggaagcggtc
     4501 gttttcgtcg atggggaagc tttggtacag cgggttgtcg gagagggcga gcaggccggc
     4561 gggggttttt tgcaggcgtt tggtgtagag gcctccggcg taggcgaaga agtagatgcc
     4621 gtcgcctgcg aaggtgtcgg ctttggtgtc gataaggacg gcggatgttt cggggatggt
     4681 gggctccatg ctgtcgccgc gcgtgccgac gattttgagg ccggaggtgt cgcggccgga
     4741 gaagaggcgg cgcacggcgt cgaggggaaa ttccacgctg ctgatggggg acggccagtc
     4801 gtcggcttcg atgccgccgc cgcacgcgcc cgccatgtcg agatggtcta tgcggatggt
     4861 ttcggtgtct tcgggaggga gcggcagggt ttctccgtga ttcatatcca accagccctc
     4921 cgccttgttc atggctgact ctaattttct cgccattgcc ccgcctatgt ttttcggctt
     4981 cccgttttgt atcgctgtct gattgatgat ttgatacaga tagctaggtt gggcatatcc
     5041 cgcacgtttt gccaattcgg caacgcttcc ggcttcctct actaaatttt tcatattctg
     5101 caaacggatt tctgcaagaa aattcatgtt tcaactccta ttgttatcga cgagataaca
     5161 atataaccaa aaattctctg ttcacaaagt tgtcaaaaga taacataata gatacaatgc
     5221 aattatctaa cggataatta agcgtttgta tgaatctgaa ctcttatcta aacagcaagc
     5281 ctcggggcga gatgacgcgc cttgctgctg caatcaatga aaaattatcg aatttatcca
     5341 gtatgagaca cggaaagaag actgtgcctc cgtacaagtg ccgccgaatt gtcgaagcta
     5401 caaacgggga agttacactt aaagacctgc gcccggacga ctggcacgaa atttggcctg
     5461 agttgaagga ggattgagat ggagattgtg ccggtgaagt gggaggacgc tttcggttgt
     5521 cccgaaggct ggcagatgct ggacggggtg gggcgggaaa ccagccttgt gcagtcggtc
     5581 ggctttgtcg cggcggaaac ggaaacgacg ctgacgattg tgccgcatgt cggcggcctg
     5641 aatcgcgaaa gaggtcggct gctcgaaaga gtttattaat aaaatcggta acggcgagcg
     5701 aaaaaaccca cgttatcaaa ttgttgattc tttaaggagt ttatacagga agaatcaaaa
     5761 ccaacccaaa taaaaagccc gtcggggatg acgggcagcc ggttacgcat tactcgattg
     5821 ttgaaaacgg ggtggaagtg tgggcgcaag accaatggga atggaaggac ggtaaatgaa
     5881 atatattcca aattcgtttc agatagcaaa cgcggtagtg gacgatttcc tctgccgaat
     5941 gagcggcaac gcgtggaaat gctacgccgt catcgtgcgc aaaacgaccg gctggcaaaa
     6001 ggaaattgac tacatctctg tttcccaatt taaaaacctg accggaatca aaacagacgt
     6061 aacagttgcc gacgcgctga aagagcttgt ggaattgaac ctgattgcct ccgtcaaacg
     6121 gcacggtcag gtaaccggct accgcatcaa tatgcccgaa ccgtcccccg aaaatggggg
     6181 tacaccaccc ccggaaaatg ggggtactgc cacccccaaa aattgggggt ctacaaaaca
     6241 cactacaaaa cccactaata caaaacacag tattagcgca tccgcagcgg cggacgcgcc
     6301 cctttctgcc gaacctcccg aaaccgcacc ggcggcgaag gcgaaaaaaa ccggcaggca
     6361 cgaaaccgag ctttcgctgc ttgccgacta cggcatcacg gggcaggtgg cgg
//
LOCUS       DBJGGI010000090         6391 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.090, whole genome shotgun sequence.
ACCESSION   DBJGGI010000090 DBJGGI010000000
VERSION     DBJGGI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.090"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..113
                     /locus_tag="ACSIGJ_001922"
     CDS             <1..113
                     /locus_tag="ACSIGJ_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HHQ1330729.1"
                     /translation="PNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
     gene            complement(191..1429)
                     /locus_tag="ACSIGJ_001923"
                     /pseudo
     CDS             complement(191..1429)
                     /locus_tag="ACSIGJ_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680490.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            complement(1609..2982)
                     /locus_tag="ACSIGJ_001924"
     CDS             complement(1609..2982)
                     /locus_tag="ACSIGJ_001924"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HHQ1330730.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGVNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKAEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTFQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYI
                     HRIGRTGRAGADGVAISLMDESEQKMFEAIKELTGNKLLIERIEGFEPRWWEQEGSKP
                     EKTETSEPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRRGHRTCALL
                     QPRYGVK"
     gene            3196..4198
                     /gene="dusB"
                     /locus_tag="ACSIGJ_001925"
                     /pseudo
     CDS             3196..4198
                     /gene="dusB"
                     /locus_tag="ACSIGJ_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680069.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
     gene            4211..4360
                     /locus_tag="ACSIGJ_001926"
     CDS             4211..4360
                     /locus_tag="ACSIGJ_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330731.1"
                     /translation="MPPFGFGRHLSAWFGGRAESRPANVENNKCRLKTSGTGFAKRLS
                     DGIPS"
     gene            4387..4626
                     /locus_tag="ACSIGJ_001927"
     CDS             4387..4626
                     /locus_tag="ACSIGJ_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HHQ1330732.1"
                     /translation="MNPATADIAQCIEQNLNQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            4629..5165
                     /gene="ruvC"
                     /locus_tag="ACSIGJ_001928"
     CDS             4629..5165
                     /gene="ruvC"
                     /locus_tag="ACSIGJ_001928"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HHQ1330733.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADEP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            5216..6085
                     /locus_tag="ACSIGJ_001929"
     CDS             5216..6085
                     /locus_tag="ACSIGJ_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HHQ1330734.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIAGLIGSLAYLLVKPRRRIGEIN
                     LAKCFPEWDEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAKCLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNNSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLQFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
BASE COUNT         1397 a         1633 c         1888 g         1473 t
ORIGIN      
        1 agcccaaccc gattgagaaa gtgtgggcga atattaagcg gtatctgcga accgttttgt
       61 ctgattacgc ccgatttgac gatgcactac tgtcctattt tgattttaat tgactatatt
      121 tgtgtgaatg atgaaataaa aatgccgtct gaacccgatt ggattcagac ggcattttgt
      181 atggcaggat ttattcgctt tccaactgac cgacccattc tgacaaggca gtcaggcgtt
      241 gttcggattt cgccacgaac gggttttcgg tatcccacgc gtagcctgcc aaaatcgaag
      301 aaagcatacg gctgatttcc gggctgcggt cgggcgcgta cacgacgttt tggaagcgga
      361 aatcatacca gccgtccaca taggtgcgga acgcgtctac gccgatcatc aggggttcgg
      421 caaattcggt ttgccaatcg gcggcttcgc ctttgagttg ttttgtcagc agatcggcag
      481 caagttcggc ggagtgcagc gcgatggtta cgcccgacga gaacacgggg tcgaggaact
      541 cggcggcatt gcccaacagc gcgaaatgcc tgccgtgcag tgatttgacg ttggcggaat
      601 agccttggat ggagcggaac ggaaaatcgt tttcccaaac ggctttgtcc aaaatttcgc
      661 tcaacatcgg gcattcgtaa acaaattttt tcaacaccgt ttccgattcg ccggcaagtt
      721 tgtcgggtgt gccgaccacg ccgacggaac agcggttgtc gccgaaggga atcagccaaa
      781 tccatacgtc gcggttgttg cggatgggtg gtaatcagga ttttgttgcg gtcgaatttc
      841 gggtgggtaa tgttgtcgtc gatgtgcgtg aaatgcgttt ggcgcggcgg caggtgcgag
      901 ggcgtttcca agtttagcag gcgcggcagc acgcgtccgt agccgcttgc gtccaagacg
      961 aatttcgcgg tcagttcata gctctcgccg gtgtcggttt cgatgttcaa gcgggcaaaa
     1021 tcgccgctgt tgtcgaacgc ggttacgccg tgcccgaaac gtacttcaac gccttgtttg
     1081 gcggcttctt caatcaggat tttgtcgaac acggcgcggc ggacttggta aaccgtgccg
     1141 gggccgtctg aaaatttatc ggtgaaatca aactcggtat agcggctgcc ccacgaaaac
     1201 gccgcaccgt ttttcaactg aaagccgggc ccggtgcgaa cggcatcggc aaaaccggct
     1261 tcttccagca tttccataca gtgcggcagc aggctttcgc cgatgacgaa gcgcggaaag
     1321 tgctgttttc caacacgcag acttgataac cttttttgcg gagcagggcg gatgcggccg
     1381 aacctgccgg acctgcgccg attacggcga catcgaattg ggtggacatt gggaaatcct
     1441 gatggtttga gtggaaaagg aaatgatatt gtacaggcag gaaatgccgt ctgaaactgt
     1501 ttgcggaaga aatttatccg gtgcgggcgg ttgctcccaa tgcgtcagtc cggtttgaaa
     1561 aatgccgtct gaaacgggaa atgttcagac ggcatttgat tttcagggtt attttacgcc
     1621 gtaacgcggt tggagcaggg cgcacgtccg gtgtccccgg cggcttcggc ggctgcgccc
     1681 ggcaattttt ccgcaggccg cgcccgcatc gtttgccgtg gtttccggcc gggtattttt
     1741 ttcgcgctga gccctggcgg attcgtagcg gttgcgttgt ctcggttcgc ttgtttcggt
     1801 tttttccggt tttgagcctt cctgttccca ccatcggggc tcgaagcctt cgatgcgttc
     1861 gatgagcagc ttgttgccgg tcagttcttt gatggcctca aacattttct gttcggattc
     1921 gtccatcagg gaaatcgcca cgccgtccgc accggcgcgc cccgtgcgtc cgatgcggtg
     1981 gatgtagtct tcgggctggg cgggcatttc gtaattgatg acgaagggca gttcggcgat
     2041 gtccagccca cgcgcggcga tatcggtggc gacgaggacg cgcaggctgc cgtctttgaa
     2101 ggcgttgagt gtttcgagcc tgctttgttg ggaacggtcg ccgtgtatcg cctgtgcgga
     2161 caggttgcgg cgcaccagtt cgcgcgttac gcggtcgacg ctttgtttgg ttttgcaaaa
     2221 gacgataact tggttcatat gcagatcgac aatcagccgt tcgagcaggt tgcgcttttg
     2281 gaaggtatcg acggcgatga tgtgttgttc gacgttggcg ttggtggtgt tttgggcggc
     2341 aacctcgacg gtttcgggcg cgttcatgaa gtcttgcgcc agtttgcgta tcggggcgga
     2401 gaaggtggcg gaaaagagca gggtttggcg ttggcggggc agcatctgca tgattttgcg
     2461 gatgtcgtcg ataaacccca tatccaacat gcggtctgct tcgtccagaa cgacgatttc
     2521 ggctttgttt aaactgatgt ttttctgttt cacatggtcg agcagccgtc cgacggtggc
     2581 gacgactatt tcgcagccgg cacgcaggtc ggcggtttgt ttgtccatgt tgacaccgcc
     2641 gaagaggacg gtatgccgca gcggcaggtt tttaatgtag ccctgcacgt tttggtcgat
     2701 ttggtcggca agttcgcgcg tcggggtcaa cacgagcata cgcacggggt gcattgcagg
     2761 cgaggtgctg gcggtggcgt aacgtttgag gcgttccaga ctgggcagca taaaggcagc
     2821 ggttttgcct gtgccggttt gcgcggcggc aagcaggtcg tgtccggcca gtgctttggg
     2881 aatggccgcg gcttggatgg gcgtcgggtt ttcgtaacct tgcgcggtca gtgcggaaac
     2941 cagttccgta cccaaaccta aagaggaaaa tggattactc atgattgtag tctttctttc
     3001 agaccttatg ccgtctgaag cgggaaaccg ataggacggg gaaataaact gcccgcctgt
     3061 gaacggcggg gacgggaatt tgtgtgcgga ttatgccata aaacggtgcc cgccccaatc
     3121 gcgggcgcgt ccggagattg gaaatcctgc ttaaaaaatg tacaatggcg cacttttttg
     3181 aaacgcgggc ccattatgca catcggcggt tattttatcg acaaccccat cgcacttgcg
     3241 ccgatggcgg gcattgccga caaacccttc cgccgcctct gtcgggcgtt tggcgcaggt
     3301 tgggcggtgt gcgaaatgct ggccagcgat ccgacgctca ggaataccgg aaaaaccctg
     3361 caccgcagtg attttgccga tgaaggcggc atcgttgccg tgcagattgc cggcagcgac
     3421 cccgaacaga tggcggatgc ggcgcgttac aacgtcggac tcggggcgca ggtcatcgac
     3481 atcaatatgg gctgccccgc caagaaagtg tgcaacgtcc aagccggtag cgcgctgatg
     3541 caggacgagc cgctggttgc cgccattttg gaggcggtgg tcaaggcggc gggcgtaccc
     3601 gttaccctca aaacccgttt gggttggcac gacgaccatc aaaacctgcc cgccatcgcc
     3661 aaaatcgccg aagattgcgg cattgccgcc cttgccgtcc acgggcgcgc gcacgcaaat
     3721 gtacaaaggc gaggcgcgtt acgaactcat cgccgagacc aaaagccgtc tgaacatccc
     3781 ggcctgggtc aacggcgaca tcacttcgcc gcaaaaagcc gccgccgtcc tcaaacaaac
     3841 cgccgccgac ggcatcatga tagggcgcgg cgcgcaaggc aggccgtggt ttttccgcga
     3901 tttgaagcat tatgccgaac acggcgtttt accgcctgcc ttgagtttgg cagaatgcag
     3961 agccgccatt ttgaaccaca tccgcgccat gcacgcgttt tatggtgaga ccgtcggtgt
     4021 gcgcatcgca cgcaaacaca taggctggta catcggcgaa atgcccgacg gcgaacgggc
     4081 gcggcgtgaa atcaaccgct tggacaatgc ggcggcgcaa tacgacacac ttgccggtta
     4141 tcttgaaagg cttgccggaa aaaccgaccg ttgggcgtgc ggctatcggg aagggtaggg
     4201 cagtattgcc atgccgccgt tcgggttcgg acggcatctg tctgcatggt tcggaggtcg
     4261 ggcggaatcc cggccggcga atgtcgaaaa caataaatgc cgtctgaaaa cgagtggaac
     4321 gggtttcgcc aaaaggcttt cagacggcat tccgtcttaa aaacatttca atcaaaagga
     4381 tatgcgatga accctgcgac tgcggacatt gcgcaatgta tcgagcagaa cttgaaccaa
     4441 tatttcaaag acctgaacgg caccgaacct tgcggcgtgt acgatatggt actgcatcag
     4501 gtggaaaagc cgctgctggt gtgcgtgatg gagcaatgcg gcggcaacca gtccaaagcc
     4561 tccgtgatgc tgggactgaa ccgcaatacc ttgcgtaaga aactgattca acacggtttg
     4621 ctgtgaatat gtcggcaacc gtccgtatct tgggcatcga cccgggcagc cgcgtaacgg
     4681 gtttcggcat catcgatgtc agggggcgcg atcattttta cgtcgcctcc ggctgcatca
     4741 aaacgcctgc cgatgagcct ctggcagaca ggattgccgt gattgtgcgg catatcggcg
     4801 aagtcgttgc cgtttacaag ccgcaacagg cggcggtgga acaggtgttc gtcaacgtca
     4861 atccggcatc gacgctgatg ctcggtcagg cgcgcggagc ggcattggcg gctttggtca
     4921 gccataagct gcccgtttcg gaatacacgg ccttgcaggt caaacaggcg gtggtcggca
     4981 aaggcaaggc ggcgaaagaa caggtgcagc atatggtggt gcaaatgctg ggactttcgg
     5041 gaacgccgca ggcggatgcg gcggacggtc ttgccgtcgc gctgacccac gccttacgca
     5101 accacgggct tgccgccaaa ctcaatcctt cggggatgca ggtcaagcgc ggaaggtttc
     5161 aatagtttca gacggcattt gtattttgcc gcctgaaaag aaaatgtgta ccgagatgaa
     5221 atttatattt tttgtactgt atgttttgca gtttctgccg tttgcgctgc tgcacaagat
     5281 tgccggcctg atcggttcgc ttgcctacct tctggtcaaa ccgcgccgcc gtatcggcga
     5341 aatcaatttg gcaaaatgtt ttcccgaatg ggacgaagaa aagcgtaaaa ccgtgttgaa
     5401 acagcatttc aaacacatgg caaaactgat gctcgaatac ggcttatatt ggtacgcgcc
     5461 tgccaaatgc ctgaaatcgc tggtgcgcta ccgcaataag cattatttgg acgacgcgct
     5521 ggcggcgggg gaaaaagtca tcatcctgta cccgcacttt accgcgttcg agatggcggt
     5581 gtacgcgctt aatcaggatg tcccgctgat cagtatgtat tcccaccaaa aaaacaagat
     5641 attggacgaa cagattttga aaggccgcaa ccgctatcac aacgtcttcc ttatcgggcg
     5701 caccgaaggg ctgcgcgccc tcgtcaaaca gttccgcaaa agcagtgcgc cgttcctgta
     5761 tctgcccgat caggatttcg gacgcaacaa ttcggttttt gtggattttt tcggcattca
     5821 gacggcaacg attaccggct tgagccgcat tgccgcgctt gcaaatgcaa aagtgatacc
     5881 cgccattccc gtccgcgagg cggacaatac ggttacattg caattctatc ccgcttggaa
     5941 atcctttccg agtgaagacg cgcaagccga cgcgcaacgt atgaaccgct ttatcgaaga
     6001 acgcgtgcgc gaacacccgg aacaatattt ctggctgcac aagcgtttca aaacccgtcc
     6061 ggaaggcagc cccgattttt actgactaca taaaattaca aaacaaatca ggcgtttcag
     6121 atcaaaaacc ccgattgttt ttgggaattt gaaacccggg ttgtacaaac aggatttgcc
     6181 ggacggtttt aacggttcag ttgtttgtaa aaacaatgct tttttaaaat tgacaaaaaa
     6241 cgaaatcggt tttaaaggct tattccgaga acaaagggga gtggatgccg aaaacccggt
     6301 taatatatta tagtggatta acaaaaacca atacggcgtt gcttcgcctt agctcaaaga
     6361 gaacgattcc ctaaggtgct gaagcaccaa g
//
LOCUS       DBJGGI010000091         6024 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.091, whole genome shotgun sequence.
ACCESSION   DBJGGI010000091 DBJGGI010000000
VERSION     DBJGGI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.091"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..194
                     /locus_tag="ACSIGJ_001930"
     CDS             <1..194
                     /locus_tag="ACSIGJ_001930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330735.1"
                     /translation="NLCSLILLNFTNKKTARLFSSSNFKFVVCSGRTVSAKGSYSFAV
                     SDFIWVAGFGKDSCCDPNV"
     gene            complement(492..851)
                     /locus_tag="ACSIGJ_001931"
     CDS             complement(492..851)
                     /locus_tag="ACSIGJ_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330736.1"
                     /translation="MIHKPRYIKIVDENGDFTRVLRLHKFPDTSKVFYFEPMFWLKDG
                     RLARKDSLFEVDYIYGADGCGFLPSNLTEFRKYCRKKHQKFKDDEVLVNRYAVDFLGA
                     KEPPYDDRHVTSVKYFV"
     gene            complement(972..2044)
                     /locus_tag="ACSIGJ_001932"
                     /pseudo
     CDS             complement(972..2044)
                     /locus_tag="ACSIGJ_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
     gene            complement(2054..2344)
                     /locus_tag="ACSIGJ_001933"
     CDS             complement(2054..2344)
                     /locus_tag="ACSIGJ_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HHQ1330737.1"
                     /translation="MPLLAGLIPLLGILLKMLIVRIILATGLTFVTYAGYLAALEKFK
                     GYTANAINSMPSDILNLLLISGFGQGLGYLFGAFSFFIGMHAFKKLTFVFPG"
     gene            complement(2323..3273)
                     /locus_tag="ACSIGJ_001934"
                     /pseudo
     CDS             complement(2323..3273)
                     /locus_tag="ACSIGJ_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
     gene            3477..3761
                     /locus_tag="ACSIGJ_001935"
     CDS             3477..3761
                     /locus_tag="ACSIGJ_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HHQ1330738.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYAGVDRLAAHFQQAIT
                     HSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            3762..4949
                     /locus_tag="ACSIGJ_001936"
     CDS             3762..4949
                     /locus_tag="ACSIGJ_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin family protein"
                     /protein_id="HHQ1330739.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKPDENGVRRKVFTNI
                     KGLKIPHTHIETDAKKLPKSTDEQLSAHDMYEWIKKPENVGAIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGPKLLDQNLRTLVKRHYHIAANKMGLRTLLEW
                     KVCADDPVKMASSAFSSIYTLDKKVYDLYESAEIHTVNKVKRSKWFYALPVIILLIPL
                     FVGLSYKMLGSYGKKQEEPAAQESAATEQQAVLPDKTEGESVNNGNLTADMFVPTLPE
                     KPESKPIYNGVRQVRTFEYIAGCIEGGRTGCTCYSHQGTALKEVTELMCKDYVKNGLP
                     FNPYKEESQGQEVQQSAQQHSDRAQVATLGGKPQQNLMYDNWEERGKPFEGIGGGVVG
                     SAN"
     gene            5164..6024
                     /locus_tag="ACSIGJ_001937"
                     /pseudo
     CDS             5164..6024
                     /locus_tag="ACSIGJ_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNgo3 family transposase"
BASE COUNT         1713 a         1389 c         1395 g         1527 t
ORIGIN      
        1 agaatctgtg ttctttgata ctactcaatt tcacaaacaa gaaaaccgcc cgcctattct
       61 cgtcatcaaa ctttaagttt gtggtttgtt caggccggac ggtttcggca aagggtagct
      121 attcctttgc cgtgtctgat tttatttggg ttgcaggttt tggtaaagat tcctgttgcg
      181 acccgaatgt ctgatttttt ttaggcgtat ctcagtccgg aatcactccg ttagtgggtt
      241 tgcggtattg aaaaacagtt cataaaaaag gaaaaggggg tattcgtaaa gattgggtaa
      301 cgcacccaat ctttacaaag cttccccctt ttcctttttt ccgccctatt ttcctgcacg
      361 tacaaccccc gaacgaagcg attccggact gagatacgcc caaaaaaaaa cagccattct
      421 agcagttaac ccccttcgct ccgcccaagc catcctgagg ggtagtggct gaatttgtga
      481 ttttggtttt atcaaacaaa atatttgact gaagtcacat ggcggtcgtc atatgggggt
      541 tccttcgcac ccaaaaaatc gaccgcgtaa cggtttacta aaacttcatc gtccttaaac
      601 ttttggtgct ttttccggca atattttctg aactccgtta aatttgacgg caagaacccg
      661 caaccgtctg caccgtaaat gtaatcaact tcaaacaaac tgtctttccg ggcaagccga
      721 ccatctttaa gccagaacat aggctcgaaa taaaaaactt tcgacgtgtc cgggaactta
      781 tggagacgga gaacacgtgt gaaatccccg ttttcatcta caattttgat atatcttggt
      841 ttgtgaatca tgacatcctc agatttagta ttcagaatat gattttaaaa agaactttct
      901 gctttacgac tccgccgccg attccttcaa acggttttcc gcgctcttca gttgtcgtac
      961 attaaatttt attaggactt tccgcccatt acgagaactt ggggcttgtc cgctttcgcg
     1021 gactgtgccg cctgttccgt cctttgccgt tcgtccttgt aaggattgaa aggcaacccg
     1081 tttttcacat attctttaca cattatcttt gttatttctt tcaagggtgt tccttgattt
     1141 gaatagcatg tgcaatctga ttttccgccg tctatgcatc cggcgatttg ctcaaaggtt
     1201 tttacttgtc ggactgtgtt ataaataggc ttgctttcgg gcttttcggg caaagtcggc
     1261 acaaagtctt caggtttcag attgtcggaa tgctcaaaag gcgctgtttc tgatgatgcc
     1321 gtctgctccg tcatcgtctg cacaacgctt tctttttgcg cttcctgctc aatccggctg
     1381 tctgtggctt tgctgtaaac ttgaaacatg ccgtaacttt tccagcctac aaaccctaca
     1441 accgcaatca acgcccaaac cgcccaaggc acttttttct tgaacttttg gtgccggctt
     1501 gatgatttat agtatttgaa ggcttcttta ggcggtttcc aatttgcggc ttctacgccg
     1561 cttacgcccg cgggattgtc caacgaggtt acgcatttat accaataata ctgtttcatg
     1621 ccgattgcct tgcgttcaag gtgtacatgc tttgaaacaa ggttgcggac gaatatatca
     1681 agttggctcg ggtgccgcgt catcaaaatg acggtatgcc cgtgatggcg gagttctgtc
     1741 agttcctgaa tatagggcgg aacgggacgg cctgccgcgc gtaccgggta agtgtagtgc
     1801 gcttcgccaa caatcagcac cgcgccttcc ggtatgacat cacgaagcgg ggcggacatg
     1861 atttgccctt ccgccagttc gcgggcattg aattttcgtt tgtccaatcc gtcgatatgg
     1921 cagaaataaa gcggccggtc tgcctccgtg ccgtcttcca atttcatttt gaacaatccg
     1981 tcttcgttgt tcaaaatcat agggttttgc ctgcccccat gtttcccgta aacagataaa
     2041 tcatgcttct acctcatccc ggaaagacaa acgtcagttt tttgaatgcg tgcataccaa
     2101 tgaagaacga gaatgcgccg aacaggtagc ccaacccctg accgaatccc gaaattaaaa
     2161 gaaggttcaa tatgtcggaa ggcatggaat tgatcgcatt tgccgtgtag cctttgaact
     2221 tttccagtgc ggcgagatac ccggcatagg ttacgaatgt cagacctgtt gcaaggatga
     2281 ttctgacaat cagcattttc agaagtatgc ctaaaagtgg aatcaggccg gcaagtaatg
     2341 gcatttattc ccccccaacg aaccgaaaac gacaaaagcc gacataatga taaaggcgag
     2401 cagtacggca aaacggattt tttcggcaaa cacgcacaac ggttcatagc ttgcccgata
     2461 ttgcctgccg aaaacatgaa aggttttcgg ctgcggacat acgccgttag acggtaaaaa
     2521 gttatgtgaa gaccatgttt tatcgtctgt aacctgcggt atgcttatat cgtgaaacat
     2581 gcggtccgaa ggtttgccca tctcctgaca ggctaggatt tccggaaaat aatcgcacga
     2641 aagcccgccg tcttcgcctt gtttcctttc tttgcgatgc ctgccgtttg ggcggtccgg
     2701 aacggccggg gaatcggggc ttgttccggg ctgtccgtcc gtatcgggat ttgcatcggg
     2761 attcaaatcg ggatcgggtt cgggattggg acgcgtgccg gggttctcat cggggtccgg
     2821 gttgtttgcg gggttttccg cgggcgatac ttcgggcggc ggctgtgcgt gaggtgcttc
     2881 cgcgcttgcg ggcgtgaggt cgggacgcgg gattacttga acatccgccg tggtgttgcc
     2941 ttgcgcgtcc ctgccgaatg ttgcggcaac ctgaacggga ttcccgttcc tgtccgtgac
     3001 gggccccata ttcacttttg ttccgggtgc gacttctact ttttcggaat aaccgggata
     3061 tccggttgcc tttatgtatt tgtcgggatc ggcatcgact ttcaacgata aaatctcttc
     3121 cggctttttg gcatccattt cttctttgta tttcggattg cggccaaggg aaaaagaagc
     3181 cccagctctg aaatcatcac ctttattgac cgcacaacct ccgccgttcc aatcaaatgt
     3241 gcaacgattt aaaacaaaat tattccaatc caattaagca actttagcca aaggcaggcg
     3301 aagcatcgca ctttgcgggc gaagccgcaa acagccgaga agcgcggggg gggattggcg
     3361 ataagcgcga gggggtgtcc ccacagcgct gccgcgccgc gaatgcggag caatctttca
     3421 gattaagaaa catttgttta atgaggcaaa cgtgcctttt aagaaaggga gagcaaatga
     3481 aattgttggc cgcattgatt ccgctcttga tgagcgtcgc aggccgtata ttgactgcat
     3541 taggcttgat ggctgttacc tatgcgggtg tagatagatt ggcagcccat tttcaacagg
     3601 cgataaccca tagcataacg ggcgcgcctc aagcaatgtt acagcttttc tatataagcg
     3661 gcggtggtac tgttctaaac attcttttcg gcgcaatcgc ctttattctg tcctttaaac
     3721 aaatgacaaa actcgcaacc tcaatcggga agaaaaaata aatggcagaa atctgtttga
     3781 taaccggcac gcccggttca gggaaaacat taaaaatggt ttccatgatg gcaaacgatg
     3841 aaatgtttaa gccagatgaa aacggcgtac gccgtaaagt atttacgaac atcaaaggtt
     3901 tgaagatacc gcacacccac atagaaacag acgcaaagaa gctgccgaaa tcaaccgatg
     3961 aacagctttc ggcgcatgat atgtatgaat ggatcaagaa gcctgaaaac gtcggcgcaa
     4021 tcgttattgt cgatgaggcg caagacgtat ggcccgcacg ctccgcaggt tcgaaaatcc
     4081 ccgaaaacgt ccaatggctg aacacacaca ggcatcaggg catagatata tttgtattga
     4141 cacaaggtcc taaactctta gatcagaact tgcgaacatt ggttaaaaga cattaccaca
     4201 ttgcggccaa caaaatgggt ttgcgtaccc tgcttgaatg gaaagtatgc gcggatgacc
     4261 cggtaaaaat ggcatcaagt gcattttcca gtatctacac actggataaa aaagtttatg
     4321 acttgtacga atccgcagaa attcacacgg taaacaaagt caagcgttca aaatggtttt
     4381 atgcattgcc cgtcatcata ttattgattc cgctatttgt cggtttgtct tacaaaatgt
     4441 tgggcagtta cggaaaaaaa caggaagaac ccgcagcaca agaatcggcg gcaacagaac
     4501 agcaggcagt acttccggat aaaacagaag gagaatcggt gaataacgga aaccttacgg
     4561 cagatatgtt tgttccgaca ttgcccgaaa aacccgaaag caagccgatt tataacggtg
     4621 taaggcaggt aaggaccttt gaatatatag caggctgtat agaaggcgga agaaccggat
     4681 gcacctgcta ttcgcatcaa gggacggcat tgaaagaagt gacggagttg atgtgcaagg
     4741 actatgtaaa aaacggcttg ccgtttaacc catacaaaga agaaagccaa gggcaggaag
     4801 ttcagcaaag cgcgcagcaa cattcggaca gggcgcaagt tgccaccttg ggcggaaaac
     4861 cgcagcagaa cctaatgtac gacaattggg aagaacgcgg gaaaccgttt gaaggaatcg
     4921 gcgggggcgt ggtcggatcg gcaaactgaa gaaaacggca acagagaaaa aagacccgta
     4981 aaccgtttga atatagacgg cttacgggtc tttgtttcgc gcaaagcaag ggctaaggca
     5041 gtcaggcagc aaatcccgca atgtattaaa acagacgcgt agaaatgccg gctgccttta
     5101 tccatcctca aaattgaata tcatcctagc cgtatcaagg ctgtataaat aaggaaaata
     5161 ccaatgaata taatcgggtc ggacatctca aaggacacca tagacgcaac attgcataaa
     5221 acaaacggaa gtatccatta cattaaattt aagaataatg atgatggatt aaaacagttt
     5281 agattgtgga taaagggaaa cagaatcaga aaagtctata tcggcatgga ggcaacaggc
     5341 atctattacg aaaaggcagc agatatgctt tcttcctact ataccgttta cgttatcaat
     5401 cccttaaaaa tcaaggacta cggaaaaagc aggtttaacc gcaccaaaac cgacaaagca
     5461 gattcaaacc tgatagcaga ttacataaaa aggcatcaag atacattgat accgtatcag
     5521 atacccaaaa acaaagcact gcaaaaactg attaatctta aaaaccaatt acagcaacag
     5581 cagaagcaaa ttaaaaaccg tcttcatagc actgaagaag acttcataag gaacatacat
     5641 caagacttga tagataccat acaggacaag atggaacagg taaaaaatag ccatatccga
     5701 acaaatcaaa aaacaaacgg acaataacca ttaccgcaat cttcaaacca tcccgagcat
     5761 aggcaaagac accgcatcag ttctttatgc gcaactgaca gaaaaacatt ttaaaaccgc
     5821 aaaccaattt gtatcctatg ccggattaag tcccgccatc atacaatcag ggacaagcgt
     5881 aagaggtcgg ggcagattga gccgatacgg aaacagacga ttaaaaagta cgctgtatat
     5941 gcccgccctt tgtgcttacc gttttaacgc atttccgaaa ttaataaata atctgaaaaa
     6001 agcgggtaag ccaaagatgg taat
//
LOCUS       DBJGGI010000092         6002 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.092, whole genome shotgun sequence.
ACCESSION   DBJGGI010000092 DBJGGI010000000
VERSION     DBJGGI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.092"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(456..611)
                     /locus_tag="ACSIGJ_001938"
     CDS             complement(456..611)
                     /locus_tag="ACSIGJ_001938"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330740.1"
                     /translation="MPSESPSDGIGFPKHSAPLSSCRKQTPIRFAANAQKTQCRLKRQ
                     TGFRRHR"
     gene            complement(628..2424)
                     /locus_tag="ACSIGJ_001939"
     CDS             complement(628..2424)
                     /locus_tag="ACSIGJ_001939"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HHQ1330741.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVVTANEAGVWVDSRYWEQAAKQLSGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLAAKNIRIQHPDDLLDQVWTSRPAIPAETVFIH
                     DHAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDSAVLFTEQCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESARLIEGINPSTFFKSVKSEADIARIREAMEHDGAALCGFFAEFEDIIGKGG
                     SLTEIDVDTMLYRHRSARPGFVSLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVADPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIEWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2883..4136
                     /locus_tag="ACSIGJ_001940"
     CDS             2883..4136
                     /locus_tag="ACSIGJ_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HHQ1330742.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QLALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSCVYLWVFLILCIASATINA
                     GAVAIVTAAIVKMAAPSLMFDAGTVAALIMASCLIIVVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRIRPSGYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGREKPGNAELFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDKRHRLTAGMNA
                     LAIVGLLYLAGFAVLFLLNLTGLLA"
     gene            4291..5802
                     /gene="lysS"
                     /locus_tag="ACSIGJ_001941"
     CDS             4291..5802
                     /gene="lysS"
                     /locus_tag="ACSIGJ_001941"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HHQ1330743.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLNNIRQQRNAYPNDFKRDS
                     FAADLQAQYGEIGKEELDPQAVPVKIAGRMMLKRQMGKASFATIQDVTGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKSQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDLQTIRDVILFPQMRPE"
BASE COUNT         1274 a         1579 c         1764 g         1385 t
ORIGIN      
        1 tgggagaggg ctggagagag ggcaacaagc cgcaaggctt gtattggggc ggttaagctg
       61 ttgggaaaga ttgccgcaac ttggagaatg ccgcctcccc ggccctcccc cacgggggag
      121 ggagcagatt gcggcagatt tggcggttgc aggcggtttg atttgcgagg gaatcggacg
      181 cgcaggcggg aatccggatt tgtccgcgcg gaaacttata tttcgtcatt ccaccaatcc
      241 cgcccacagc ccacttttcc gcgtcattcc cgcgcaggcg ggaatccagt ccgttcggtt
      301 tcggttattt ccgataaatt cctgctgctt ttcattccta gattcccact ttcgcgggaa
      361 tgacgggatt tcagggtaaa gatggcggga gccggagggc tgtgaagagc tgttttcgca
      421 ggaatgacga tatgcggatt tgctgttttc atccgctacc gatgccgtct gaagcctgtt
      481 tggcgtttca gacggcattg ggttttttgt gcatttgccg caaaacggat cggtgtctgc
      541 ttgcggcaag acgataacgg tgcggaatgt tttgggaaac caatgccgtc tgaagggctt
      601 tcagacggca tttttgtacc gtgccgttta acgcgccagc ggttcggtgc gtttgatcag
      661 ccacgctttt gccgcgcctt cggtcagcgg ctcgaggcgg cggcggactt cggcgtggta
      721 gcggttgacc cattcgattt cgccgtcggt catcagggcg gtgtccatca ggcgtgtatc
      781 gatggggcag agggtcaggg tttcaaaaca gaggaagctg ccgaactcgg tttcctgcgg
      841 gtcggcgacg gcttggttgg cggcgaggtt ttcaatgcgg atgccccatt ttcccggacg
      901 gtagaggccg ggttcgatgg aggtaaccat gccttttttc atggcggttt cgggcgtggc
      961 gggggcggcg aaggcgatgc gctgcgggcc ttcgtggacg ttgaggaaat agcctacgcc
     1021 gtggccggtg ccgtgtccgt agtcgcattg cgcctgccac aggggtttgc ggcaaatcgc
     1081 gtcaatcagc ggcgagggga tgttttcggg gaacacggct tcggcaagcg cgatatgggc
     1141 tttgagaacg agcgtgttgt cgcgtttttg ttctgctgtg ggcgtgccga cggggacgac
     1201 gcgggtgatg tcggtcgtgc cgcctttgta ttgcgcgccg gagtcgatga gcagcagacc
     1261 gttgccgctg atggtgctgt ggctttcggg tgttgcgctg taatgcggca gggcgccgtt
     1321 ggcgttgaag cctgcgatgg tgtcgaaact caaggacaca aaacccgggc gcgcgctgcg
     1381 gtggcggtaa agcatcgtat ccacgtcgat ttcggtcagg ctgccgcctt tgccgatgat
     1441 gtcttcaaac tcggcgaaga aaccgcacaa tgccgcgccg tcgtgttcca tcgcttcgcg
     1501 gatgcgggcg atgtcggctt cggatttgac ggatttgaag aaggtggacg ggttgatgcc
     1561 ttcaatcagg cgcgcgcttt cgggcaggcg cacgagcgta ctgacggcgg ttttgttcgg
     1621 ctcgatgagc agcgcgccgc cgatttgcgc gagtttgtcg gcaacttggg cgtaaggctc
     1681 gaccgtgata cctgcggttt gcagcgcggc ggcggcttcg gcgttcagac ggcattgttc
     1741 ggtaaacagg acggcactgt ctttgccgat cagcaggaag gacacgaaaa cagggttgaa
     1801 aggcacgtcg ctgccgcgca ggttggtcag ccaagcgatg tcgtcaagcg aggaaaccaa
     1861 gtggtaatcc gcgccttttt ccgccataac tgcgcgcacg cgggcgagtt tctcggcggc
     1921 ggtttcggag acgtaggcgt ggtcgtggat gaacacggtt tcggcgggga tagcgggacg
     1981 gcttgtccac acttggtcaa gcaggtcgtc ggggtgttgg atgcggatgt ttttggcggc
     2041 gagcgattgc gccaaagtgc gtttgccggt gagcgacacc atatcggaag ggatgccgac
     2101 ggcagcgttt tcgggcaggt ttgccgcgag ccattcgttg tacggcggca cttgcccgct
     2161 tttttgcagc tcaatgccgc tgccggaaag ctgtttggcg gcttgttccc aatagcggct
     2221 gtccacccat acgcccgctt cattggcggt aacgacgaat gtgccgaccg agccggtaaa
     2281 gcctgacaat tcgcgccgcg cctgccaatg ctcgggcagg tattcggata ggtgggggtc
     2341 ggcggaaggg atgacgagtg cgtccaagcc ttgcgccttc atggcttcgc gcaatgcgga
     2401 caggtaattc gatacggtat tcataacagt tcctccaatc gggtttcgcg gcttcagacg
     2461 gcatggacgg aaatctgaaa tgccgtctga aaaacataac catattatca aatcgccagc
     2521 ctgctgcaaa acggacgggc tgatcggatg gtgcgtgttt gcacgaaaaa aagcgatgta
     2581 aggttgtttg ttgtaaaatt tcaggcataa aatgtcgttt catgccttct tttggaagta
     2641 atacggaaaa tatagtcagg tttcagcatg aagttctaat agtgtgtgtt ggcaggtatt
     2701 tggattacgt tacaatacgc gctttcatca aaatttccct accgggcggt atcctgtttt
     2761 tgaagacggt aggatttggg tttgtttggg gtcaatcccg cccaaattgg agcaatagat
     2821 agggctgtgt ccgaatatgc ggctctgtat gttgaaacac agttaaaaat acggaagact
     2881 ttatgtccga acaacatatt tcgacttgga aaagcaagat caacgcattg ggaccgggga
     2941 ttatgatggc ttcggcggct gtcggcggtt cgcacctgat tgcctcgacg caggcgggcg
     3001 cgctttacgg ctggcagctc gcgctcatca tcatcctgac caacctcttc aaatacccgt
     3061 ttttccgctt cagcgcgcat tacactctgg acacgggcaa gagtctgatt gaaggttatg
     3121 ccgagaaaag ctgcgtttat ttgtgggttt ttttgatttt gtgtatcgcc tccgccacga
     3181 ttaacgcggg cgcggtcgcc attgtaaccg ccgccatcgt caaaatggcg gcaccctcgc
     3241 tgatgtttga tgccggcacg gttgccgcct tgattatggc atcctgcctg attattgtgg
     3301 taagcggacg ctatcgcgct ttggatcgtg tttccaaaat catcattgtt actttgagca
     3361 tcgccacgct tgccgccgcc ggcatcgcta tgtcgcgcgg tatgcagatg cagcccgatt
     3421 ttatcgagcc gacaccgtgg acgcttgccg gtttgggctt cctgatcgcg ctgatgggct
     3481 ggatgcccgc gccgattgag atttccgcca tcaattcttt gtgggtaacc gaaaaacaac
     3541 gcatccggcc ttccggctac cgcgacggca ttttcgattt caacgtcggt tatatcgcca
     3601 gtgcggtttt ggctttggtt ttccttgcac tgggcgcgtt tgtgcaatac ggcaacggcg
     3661 aagcagtgca gatggcgggc ggcaaatata tcgggcaatt gattaatatg tatgccgtaa
     3721 ccatcggcgg ctggtcgcgc ccgctggtgg cgtttatcgc gtttgcctgt atgtacggca
     3781 cgacgattac cgttgtggac ggctacgccc gcgccattgc cgagcccgtg cgcctgctgc
     3841 gcggcaggga aaaacccggc aacgccgaac tctttgcctg gaatatttgg gtggcgggca
     3901 gcggtttggc ggtgattttc tggttcgacg gcgtaatggc gaatctgctc aaatttgcga
     3961 tgattgccgc ctttgtgtcc gcccctgtgt tcgcctggct caactaccgc ctcgtcaaag
     4021 gggacaaacg ccacaggctt accgccggta tgaacgccct tgccattgtc ggcctgctct
     4081 acctggccgg gtttgccgtt ttgttcctgc tgaacctgac ggggcttttg gcatagtttc
     4141 caaaacacgg atgccgtctg caagattttc agacggcatc gccctttctt ggaaaaaaac
     4201 accgacatcg cgtaaaatac gcgcaaattt tgtgattcgg tcaggtcgtc tgaaacagac
     4261 tgcgcctgat ttattttttc ggaaaaccct atgagcgaac aaaaccatcc gcaaaccgag
     4321 ccgcagttgg acgaaaacca aatcatcgcc ctgcgccgcg aaaaactgaa caacatccgc
     4381 caacagcgca acgcttaccc caacgatttc aagcgcgaca gctttgccgc cgatttgcag
     4441 gcgcaatacg gcgaaatcgg caaagaagaa ctcgacccgc aggccgttcc cgttaaaatt
     4501 gccggccgca tgatgctcaa acgtcaaatg ggcaaagcca gcttcgccac cattcaagac
     4561 gttaccggtc aaatccagct ttatctgaac aacaaaggcg tgagccaaga agttttggac
     4621 gacttcaacc actgggactt gggcgacatc gtcggtgcgg aaggcacttt gttcaaaacc
     4681 aaccacggcg aactaaccgt gcgtgtgtcc gacatccgcc tgctgtccaa atccctgcgt
     4741 ccgctgcccg acaaacacaa aggcttgagc gatcaggaaa ccaaataccg ccaacgctac
     4801 gtcgatttga ttgccaatga agaatcgcgc aataccttta tcaaacgcag ccaaatcatc
     4861 caatctgtgc gtaattttat ggtgggcgag cattatctcg aagtcgaaac cccgatgatg
     4921 cacccgattc ccggcggcgc gacagcgaaa cccttcgtta cccaccacaa tgccttggac
     4981 attccgctct acctgcgcat cgcgcccgag ctgtatctga aacgcttggt ggtcggcggc
     5041 ttggaacgcg tgtttgaaat caaccgcagc ttccgtaacg agggcatgtc cgtgcgccac
     5101 aaccccgaat ttacgatgat tgaattttac gaagccttct ccgactacga acgtatgatg
     5161 cagatggcgg aagacatcat ccgcaacgca tcgcgcacgg taaacggcac ggcaaacatc
     5221 acttacaacg gcaaagaagt cgatttggaa agcccgttcg aacgtctgac catcctcgaa
     5281 gccatcaaaa aatacaatcc gcactacacc gacgagcagt tgaacgatgc ggaatggctg
     5341 aaaaaagaaa tcgtcaaaca cggcgaaagc ctgccgccgt ccccgggcat cggcagcctg
     5401 caactcgccc tgtttgaagg ttgcgccgag ggcaaactgt ggaatccgac ctttattgtc
     5461 gattacccgg tcgaagtttc cccgttggcg cgcgcttcgg atacgaaaca aggtctgacc
     5521 gaacgcttcg agcttttcgt tgtcgggcgc gaactggcaa acggctattc cgagttgaac
     5581 gaccccgaag accaagccga acgcttcaaa tcgcaagtgg cgcaaaaaga tgcgggcgac
     5641 gacgaagcca tgcactacga tgccgactac atccgcgcga tggaattcgg tttgccgccg
     5701 accggcggtt gcggcatcgg catcgaccgc ttagtgatgc tgctgaccga tttgcaaacc
     5761 atccgcgatg tgattctgtt tccgcagatg cgtcccgagt aatcatatat tcaattaaaa
     5821 acaaaatagt acaacactcg acgttgaagg tttaaccatg gcatactctg cggacttaag
     5881 aaacaaagct ttaaaccata gcggattaac aaaaatcagg acaaggcggc gggccgcagg
     5941 cagtacgaat ggtacggaac cgatccgccc ggcgctccat caccttaggg aaccgttccc
     6001 tt
//
LOCUS       DBJGGI010000093         5659 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.093, whole genome shotgun sequence.
ACCESSION   DBJGGI010000093 DBJGGI010000000
VERSION     DBJGGI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.093"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(426..3179)
                     /gene="tbpA"
                     /locus_tag="ACSIGJ_001942"
     CDS             complement(426..3179)
                     /gene="tbpA"
                     /locus_tag="ACSIGJ_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HHQ1330744.1"
                     /translation="MQQQHLFRFNILCLSLMTALPAYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIRTGRRAGEIRAHEAAGRGVQSFNRLVPVEDDNQYAHFVVEEECESGSYETCKSKP
                     KKDVVGKDERQMVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILERT
                     QQTFDTRDMTVPAFLTKAVFDENKKQAGSLSGNGKYAGDHKYGGLFTNGENGALVGAE
                     YGTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADG
                     SDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGS
                     NLRHQDYYYQNANRAYSLKTPPQSGNGGSNANPNGSQDKPYWVSIGGGNVVTGQICRL
                     GNNTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHR
                     TLSWNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRSGDKIKAVKIDPEKSFNKEA
                     GIVFKGDFGNLEASWFNNAYRDLIVRGYEAQIKNGKEEAKGDPAYLNAQSARITGINV
                     LGKIDWNGVWDKLSEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGLG
                     YDQPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYY
                     TIKKHFTLRAGVYNLLNHRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLE
                     MKF"
     gene            complement(3266..5341)
                     /locus_tag="ACSIGJ_001943"
     CDS             complement(3266..5341)
                     /locus_tag="ACSIGJ_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HHQ1330745.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRAAPKY
                     QDVPSKKPEARKDQGGYGFAMRFKRRNWHRMANENEVKLNESDWEQTDNGNIKEPSKQ
                     KSIIDALTGNDGETLQDSSQQEGISKVTGYHDFKYVWSGFFYKHIRTKSETIDGKVTV
                     RSGPDGYIFYKGTDPSRKLPVSGKVMYKGTWDFLTDVKANQKFTDLGNASAKPGDRYS
                     AFSGELDYIVKKEDDKKDGHVGLGLTTEITVDFGKKTLSGKLIKNNMVINNGDEPTTQ
                     YYSLEAQVTGNRFNGKAIATDKPKANETKEHPFVSDSSSLSGGFFGPKGEELGFRFLS
                     DDGKVAVVGSAKTKDETASSGGTSGGASVSASGGTTGTPSENKLTTVLDAVELTPDGK
                     KIKDLDNFSNAAQLVVDGIMIPLLPTESGNGQADKGENGKTAFIYETTYTPESDKKDT
                     QTGMATNGVQTVSNTAGGTSGKTKTHYKVQACCSNLNYLKYGLLTRKNSESAMQAGES
                     SSRTAVQTAQGAQSMFLQGERTDEKEIPKDGNVVYLGTWYGHIAINGTSWTREASNQE
                     NGNRAKFDVNFKDKKITGTLTAANRQEATFTIDAMIEGNGFKGTAKTGDGGFAPDQNN
                     STGTHKVHIAEAKVQGGFYGPNAEELGGWFAYPGNGQAENAQTSSGNGNSAGSATVVF
                     GAKRQELVK"
BASE COUNT         1175 a         1460 c         1407 g         1617 t
ORIGIN      
        1 taaattgcct tagtattgaa tgtctagatt cccgcctgcg cgggaatgac ggattttagg
       61 ttggcgtcat ttattggaaa aagcagaaac cgctccgccg tcattcccac gaaagtggga
      121 atccagtttt ttgagtttca gtcatttccg ataaattgcc ttagcattga atgtctagat
      181 tcccgcctgc gcgggaatga cgggatttga ggtttctgtt tttgattttc tgttttcgta
      241 ggaatgacgg tttagagtta cccgaaacct caaaaaaaac cgaaaccgaa cggactggat
      301 tcccgcctgc gtgggaatga cgggtttcaa gattacggta ttgtcgggga tgatgggaaa
      361 cggtgggaat tgtgtaaaaa atgccgtctg aagcctttca gacggcattt gcggcatttg
      421 gacgtttaaa acttcatttc caagctaaat gtgtagtttc ggccgggggc ggcatatcgg
      481 ttgtaaacgc cgacattttt gtgttggttg actgcgccgc cggcagtttg ccgcacattt
      541 tcccaagtaa catagcggtg gttgaggagg ttgtacacgc ccgcacggag ggtgaagtgt
      601 tttttaatcg tgtaataacc ggacacgtcc acaatatacc aagggcgggt acggcgcgcg
      661 gtggcttttg tattgcggct gttgccgttg agcaaagccc ggctgcccaa caactctgtg
      721 atttccttgg ctttggaata agtcagcata ccgttcacac cccatttgcc ttccggttgg
      781 tcatagccca agccgacgac atagcgcgaa ggttggatgg catcaaacag gtgtgattga
      841 atatcggtgc ggtctgcgcg ttttttgatg tcgcggacac ggacacgatt ataggcaaat
      901 gtagaatacc aaccttcgga caatttatcc catacgccgt tccaatcgat tttgcccaaa
      961 acattgatgc cggtaatccg cgcgctttgg gcattgaggt aagccgggtc gcctttggct
     1021 tcttctttgc cgtttttaat ttgcgcttca taaccccgga caatcaaatc gcggtaggca
     1081 ttgttgaacc aacttgcctc caagttgccg aaatcgcctt taaacacgat gccggcttct
     1141 ttgttgaacg atttttccgg atcgattttg acggctttta ttttatcgcc cgaccgccag
     1201 ccgtacattt ccgcaaacga gggcaggcgg aagccggttg aagtgcggta agtcaaatcc
     1261 agccagtcgg caggtttgag gacgatgccg gcgttccagg acagggtacg gtgcgttccg
     1321 gtggaaacgc tgccgtcgtc cgaatgcgtg ctgcggtagt cgtagcgcaa ccccgcgccg
     1381 acatccgccc acctgcccaa acggacgttg tcccgaactg ccgcgtaata gcttttgccg
     1441 ttgatgctgc gcggcgtgca gtccgtataa gtattgttgc ccaagcggca gatttgcccc
     1501 gtaaccacat ttcccccgcc tatgctgacc caatagggtt tgtcttggct gccgttgggg
     1561 ttagcgtttg agccgccgtt gccgctttga gggggcgttt tcaacgaata ggcacggttg
     1621 gcattttgat aataataatc ctgatggcgg agattagagc caaagcggtc aaaaccgagg
     1681 ttgacgctca ggttgtggcg gattttggcg gtatcgaagg attttttgaa tgccgcctgc
     1741 aagagcctgt ggctttcccc gtaaatcacg cggtcggatt tgtaatagga aaacggcttg
     1801 tcggcactcg ggcggcaata tttgtccgaa ccgtcggcag aacagtgcgt ctgctgaaaa
     1861 tggttgtcca aaccgatgcc ctgccggtcg taagagaggc gggcataatc cgcccaagtg
     1921 tctttatcgg cattggtata gacatattcc aaaccgtagc ggcttttggt gtgcgtctcg
     1981 tcgtaaaaca cgcccgtacc gtattccgcg cccaccagcg caccgttttc gccgttggta
     2041 aacagaccgc cgtatttgtg gtcgcccgcg tatttgccgt tgccggacaa agaacccgcc
     2101 tgttttttat tttcatcaaa aaccgccttg gtcaggaatg ccggaaccgt catatcgcgc
     2161 gtgtcgaaag tttgttgcgt gcgttcgagt atgccgccga tgtagtgccg tttgttttca
     2221 aaacgaaaac ccgggcggaa cagccacgac cggctttcgt atgaaagcgg atcggcgagg
     2281 aagcggttgg gacccgtgta gtctcgggta gaaaccattt gacgttcgtc tttgccgaca
     2341 acatcttttt tcggtttgct tttacacgtt tcataactcc cgctttcgca ttcttcttcg
     2401 accacgaaat gagcgtattg gttgtcatct tcaaccggca ccagcctgtt aaagctctga
     2461 acgccgcgtc cggcggcttc gtgggcgcgg atttcccccg cgcgccggcc ggtgcggatc
     2521 agcaaagcct ccgcaccgcc gatgcgcccc gccagcgcga tggattgggt aagcccccgg
     2581 tttttgccgg aataggcggt tttactctga atgccccact gcctgccttc cccgataaca
     2641 tcgtcggcgg ttttggtttg aaatgcgacc gaacccgcca atgcgccgct gccttgttcg
     2701 accgagtttg agcctttgct gatttcaaca gccttgacgt tttcatactc gatttcattg
     2761 attgcgccgc tgctgcccgc cgtcctcgtc ccgcccaatg ccgcctgagc ggtgtaggac
     2821 tgtatttgcg ccaagccgtc taccgtcaag gagacgcggt ttttgtccat accgcgtatc
     2881 gagtagcccg agcttgcgcc gcgcccctgt tcgacgacgg caatgccggg gtcgtaacgc
     2941 gtcaggtctc ggatgtcgag tacctgttct ttgctgaggg tgtcggcggt tttgaccaat
     3001 ttgcccaaac cggttacttc gttatcgcgg cgggttttct gttttttggc ttttacctgt
     3061 atggtatcca actgtttttc ctgtgcttgt ccggcttgca cattttctgc ataagcgggc
     3121 agcgcagtca ttaaagacag gcataaaata ttgaatcgga acaaatgttg ctgttgcata
     3181 gtgtttccct aatcttcgct ttcagacgga atcggaagga acgatgccgt ccgaagcctt
     3241 attctcgatt gttcggcagc cgtgcttatt tcacaagctc ttggcgtttc gcaccgaata
     3301 ccacggtcgc gctgcctgct gaatttccat tgccggatga agtttgcgca ttttccgctt
     3361 gcccattgcc cggataggca aaccatccgc ccaactcttc ggcgttaggc ccgtaaaaac
     3421 cgccctgcac cttggcttct gcgatgtgca ctttatgtgt accggtgcta ttgttttgat
     3481 ccggcgcaaa gccgccatca ccggttttcg ccgtaccttt aaagccgttg ccctcaatca
     3541 tggcatcaat ggtaaaggtt gcctcctgcc tgtttgcagc ggttaacgtg ccggtaattt
     3601 ttttgtcttt gaaattcacg tcaaactttg cccgattacc attttcctga ttggaggctt
     3661 cgcgggtcca acttgtgccg ttgatggcaa tatgcccgta ccaagtgcct agataaacga
     3721 cgtttccgtc ttttggaatt tctttttcat cggtgcgctc gccttgaagg aacatacttt
     3781 gtgcgccctg tgccgtttgg acggcagttc ggctactgct ttcgcctgcc tgcatcgcgg
     3841 attcgctgtt tttgcgcgtc agcagcccgt atttcagata attgaggttg gaacagcagg
     3901 cttggacttt ataatgggtt tttgttttgc cacttgtgcc gcccgccgta tttgaaacgg
     3961 tttgcacgcc attggtcgcc atacctgttt gggtgtcttt tttatcactt tccggcgtgt
     4021 aggttgtttc gtagataaag gctgttttgc cgttttcacc tttatctgcc tgaccgttcc
     4081 cgctttcggt gggcaggagc ggaatcataa tgccgtcgac aaccagttgg gcggcgttgc
     4141 tgaagttgtc gagatctttg attttcttgc cgtctggtgt caattcaacc gcatccaaaa
     4201 ccgtggtcag cttgttttca gacggcgtac ctgtcgtacc gcccgatgcg gaaacgcttg
     4261 caccgcccga agtgccgcca ctgcttgcgg tttcgtcttt ggttttcgcg ctgccgacaa
     4321 cggcaacttt tccatcgtcg ctcaaaaagc ggaaacccaa ttcctcaccc ttcgggccga
     4381 aaaagccgcc gctcaaagaa gacgagtcgg aaacaaaggg atgttccttg gtttcattcg
     4441 cttttggttt atcggtcgct attgccttgc cgttgaagcg gttgcctgtt acttgagcct
     4501 caaggctgta gtattgggtg gtgggttcat cgccattatt gattaccatg ttgtttttaa
     4561 ttaattttcc gctcagggtt tttttcccaa aatcaaccgt tatttccgtt gttaatccca
     4621 aacctacgtg cccgtctttt ttatcatcct cttttttgac tatataatcc aactccccgg
     4681 aaaaagcact atatcggtcg ccaggtttcg cagaagcatt tcctaaatct gtaaatttct
     4741 gatttgcttt cacatcagtt aaaaaatccc aagtaccttt gtacataacc ttccctgaaa
     4801 caggaagttt tctcgaggga tctgtgcctt tataaaaaat ataaccgtca ggtccacttc
     4861 ggactgttac ctttccatca attgtttcag atttagttcg aatatgttta taaaaaaaac
     4921 ccgaccaaac gtatttgaaa tcgtgatagc ccgtaacctt agatataccc tcttgttgac
     4981 ttgaatcttg caatgtttct ccatcatttc cagttaaggc atcaataata cttttttgtt
     5041 tggaaggttc tttgatatta ccattatccg tttgttccca atcactctcg ttcagtttaa
     5101 cctcgttttc gtttgccatc cgatgccaat tccgccgctt gaagcgcatt gcaaaaccgt
     5161 atccgccttg gtcttttcgg gcttccggtt ttttggaagg aacatcttga tactttggcg
     5221 cggcacgcgg ggcttcggta tcgacagaat caagatcgaa actgccgcct ccgcccagac
     5281 aagcgctcaa caaaaacacg ggcagcacca tagcagcctg attcaccaat ggattgttca
     5341 taacgaaccc gattcaatta aagaatgata aggattatta ttttatttat ttttaaaaaa
     5401 tttgcaaatg cttttttata tttttagtaa ataaaccaaa ataccgtcat tcccgcgctg
     5461 cttttaaaac cgcctgcaac cgccaaacct gctgcaatcc gctccctccc ctgcgcgggg
     5521 gagggctggg gagagggcac tttccaagtt gcggcaacct ctcccaaatc ccttagtctt
     5581 ctaaaacaca agccttgcgg cttactgccc tctctctagc tttctcccac agggagagag
     5641 gacggtgcgg ctgttgggg
//
LOCUS       DBJGGI010000094         5551 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.094, whole genome shotgun sequence.
ACCESSION   DBJGGI010000094 DBJGGI010000000
VERSION     DBJGGI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.094"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..1187
                     /locus_tag="ACSIGJ_001944"
     rRNA            <1..1187
                     /locus_tag="ACSIGJ_001944"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1288..1364
                     /locus_tag="ACSIGJ_001945"
     tRNA            1288..1364
                     /locus_tag="ACSIGJ_001945"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1322..1324,aa:Ile,seq:gat)
     gene            1370..1445
                     /locus_tag="ACSIGJ_001946"
     tRNA            1370..1445
                     /locus_tag="ACSIGJ_001946"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1403..1405,aa:Ala,seq:tgc)
     gene            1792..4683
                     /locus_tag="ACSIGJ_001947"
     rRNA            1792..4683
                     /locus_tag="ACSIGJ_001947"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4780..4893
                     /gene="rrf"
                     /locus_tag="ACSIGJ_001948"
     rRNA            4780..4893
                     /gene="rrf"
                     /locus_tag="ACSIGJ_001948"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            4929..5045
                     /locus_tag="ACSIGJ_001949"
                     /pseudo
     CDS             4929..5045
                     /locus_tag="ACSIGJ_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
     gene            5150..5545
                     /locus_tag="ACSIGJ_001950"
     CDS             5150..5545
                     /locus_tag="ACSIGJ_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HHQ1330746.1"
                     /translation="MSRGGATPYRFLLIRHIVARCGLLFATLKGKTMKKMFVLFCMLF
                     SCAFSLAAVNINAASQQELEALPGIGPAKAKAIAEYRAQNGAFKSVDDLIKVKGIGPA
                     VLAKLKDQASVGAPAPKGPAKPVLPAVKK"
BASE COUNT         1481 a         1219 c         1657 g         1194 t
ORIGIN      
        1 cagtggggaa ttttggacaa tgggcgcaag cctgatccag ccatgccgcg tgtctgaaga
       61 aggccttcgg gttgtaaagg acttttgtca gggaagaaaa ggccgttgcc aatatcggcg
      121 gccgatgacg gtacctgaag aataagcacc ggctaactac gtgccagcag ccgcggtaat
      181 acgtagggtg cgagcgttaa tcggaattac tgggcgtaaa gcgggcgcag acggttactt
      241 aagcaggatg tgaaatcccc gggctcaacc cgggaactgc gttctgaact gggtgactcg
      301 agtgtgtcag agggaggtgg aattccacgt gtagcagtga aatgcgtaga gatgtggagg
      361 aataccgatg gcgaaggcag cctcctggga taacactgac gttcatgtcc gaaagcgtgg
      421 gtagcaaaca ggattagata ccctggtagt ccacgcccta aacgatgtca attagctgtt
      481 gggcaacttg attgcttggt agcgtagcta acgcgtgaaa ttgaccgcct ggggagtacg
      541 gtcgcaagat taaaactcaa aggaattgac ggggacccgc acaagcggtg gatgatgtgg
      601 attaattcga tgcaacgcga agaaccttac ctggttttga catgtgcgga atcctccgga
      661 gacggaggag tgccttcggg agccgtaaca caggtgctgc atggctgtcg tcagctcgtg
      721 tcgtgagatg ttgggttaag tcccgcaacg agcgcaaccc ttgtcattag ttgccatcat
      781 tcggttgggc actctaatga gactgccggt gacaagccgg aggaaggtgg ggatgacgtc
      841 aagtcctcat ggcccttatg accagggctt cacacgtcat acaatggtcg gtacagaggg
      901 tagccaagcc gcgaggcgga gccaatctca caaaaccgat cgtagtccgg attgcactct
      961 gcaactcgag tgcatgaagt cggaatcgct agtaatcgca ggtcagcata ctgcggtgaa
     1021 tacgttcccg ggtcttgtac acaccgcccg tcacaccatg ggagtggggg ataccagaag
     1081 taggtagggt aaccgcaagg agcccgctta ccacggtatg cttcatgact ggggtgaagt
     1141 cgtaacaagg tagccgtagg ggaacctgcg gctggatcac ctcctttcta gagaaagaag
     1201 gggctttagg cattcacact tatcggtaaa ctgaaaagat gcggaagaag cttgagtgaa
     1261 ggcaaggttc gcttaagaag ggaaaccggg tttgtagctc agctggttag agcacacgct
     1321 tgataagcgt ggggtcggag gttcaagtcc tcccagaccc accaagaacg ggggcatagc
     1381 tcagttggta gagcacctgc tttgcaagca gggggtcatc ggttcgatcc cgtttgcctc
     1441 caccaaaact ttacaaatga aagcaagttt gctgttttta gcagcttatt ttgatttgcg
     1501 aagtagaata acgacgcatc gatctttaac aaattggaaa gccgaaatca acaaacaaag
     1561 acaatgagtt tgttttgatt ttttattctt tgcaaaggat aaaaacctct cgcaagagaa
     1621 aagaaaacaa acatagtatt tgggtgatga ttgtatcgac ttaatcctga aacacaaaag
     1681 gcaggattaa ggcacaacaa agcagtaagc tttatcaaag tagggatttc aagtttgctt
     1741 acttagtcaa cgggtaggta aacgaagtca aagaagttct tgaaatgata gggtcaagtg
     1801 aataagtgca tcaggcggat gccttggcga tgataggcga cgaaggacgt gtaagcctgc
     1861 gaaaagcgcg ggggagctgg caataaagct atgattccgc gatgtccgaa tggggaaacc
     1921 cactgcattc tgtgcagtat cctaagttga atacataggc ttagagaagc gaacccggag
     1981 aactgaacca tctaagtacc cggaggaaaa gaaatcaacc gagattccgc aagtagtggc
     2041 gagcgaacgc ggaggagcct gtacgtaata actgtcgaga tagaagaaca agctgggaag
     2101 cttgaccata gcgggtgaca gtcccgtatt cgaaatctca acagcggtac taagcgtacg
     2161 aaaagtaggg cgggacacgt gaaatcctgt ctgaatatgg ggggaccatc ctccaaggct
     2221 aaatactcat catcgaccga tagtgaacca gtaccgtgag ggaaaggcga aaagaacccc
     2281 gggaggggag tgaaacagaa cctgaaacct gatgcataca aacagtggga gcgccctagt
     2341 ggtgtgactg cgtacctttt gtataatggg tcaacgactt acattcagta gcgagcttaa
     2401 ccggataggg gaggcgtagg gaaaccgagt cttaataggg cgatgagttg ctgggtgtag
     2461 acccgaaacc gagtgatcta tccatggcca ggttgaaggt gccgtaacag gtactggagg
     2521 accgaaccca cgcatgttgc aaaatgcggg gatgagctgt gggtaggggt gaaaggctaa
     2581 acaaactcgg agatagctgg ttctccccga aaactattta ggtagtgcct cgagcaagac
     2641 actgatgggg gtaaagcact gttatggcta gggggttatt gcaacttacc aacccatggc
     2701 aaactcagaa taccatcaag tggttcctcg ggagacagac agcgggtgct aacgtccgtt
     2761 gtcaagaggg aaacaaccca gaccgccggc taaggtccca aatgatagat taagtggtaa
     2821 acgaagtggg aaggcacaga cagccaggat gttggcttag aagcagccat catttaaaga
     2881 aagcgtaata gctcactggt cgagtcgtcc tgcgcggaag atgtaacggg gctcaaatct
     2941 ataaccgaag ctgcggatgc cggtttaccg gcatggtagg ggagcgttct gtaggctgat
     3001 gaaggtgcat tgtaaagtgt gctggaggta tcagaagtgc gaatgttgac atgagtagcg
     3061 ataaagcggg tgaaaagccc gctcgccgaa agcccaaggt ttcctacgca acgttcatcg
     3121 gcgtagggta agtcggcccc taaggcgagg cagaaatgcg tagtcgatgg gaaacaggtt
     3181 aatattcctg tacttgattc aaatgcgatg tggggacgga gaaggttagg ttggcaagct
     3241 gttggaatag cttgtttaag ccggtaggtg gaagacttag gcaaatccgg gttttcttaa
     3301 caccgagaag tgatgacgag tgtctacgga cacgaagcaa ccgataccac gcttccagga
     3361 aaagccacta agcttcagtt tgaatcgaac cgtaccgcaa accgacacag gtgggcagga
     3421 tgagaattct aaggcgcttg agagaactcg ggagaaggaa ctcggcaaat tgataccgta
     3481 acttcgggag aaggtatgcc ctctaaggtt aaggacttgc tccgtaagcc ccggagggtc
     3541 gcagagaata ggtggctgcg actgtttatt aaaaacacag cactctgcca acacgaaagt
     3601 ggacgtatag ggtgtgacgc ctgcccggtg ccggaaggtt aattgaagat gtgcaagcat
     3661 cggatcgaag ccccggtaaa cggcggccgt aactataacg gtcctaaggt agcgaaattc
     3721 cttgtcgggt aagttccgac ccgcacgaat ggcgtaacga tggccacact gtctcctccc
     3781 gagactcagc gaagttgaag tggttgtgaa gatgcaatct acccgctgct agacggaaag
     3841 accccgtgaa cctttactgt agctttgcat tggactttga agtcacttgt gtaggatagg
     3901 tgggaggctt ggaagcagag acgccagtct ctgtggagtc gtccttgaaa taccaccctg
     3961 gtgtctttga ggttctaacc cagacccgtc atccgggtcg gggaccgtgc atggtaggca
     4021 gtttgactgg ggcggtctcc tcccaaagcg taacggagga gttcgaaggt tacctaggtc
     4081 cggtcggaaa tcggactgat agtgcaatgg caaaaggtag cttaactgcg agaccgacaa
     4141 gtcgggcagg tgcgaaagca ggacatagtg atccggtggt tctgtatgga agggccatcg
     4201 ctcaacggat aaaaggtact ccggggataa caggctgatt ccgcccaaga gttcatatcg
     4261 acggcggagt ttggcacctc gatgtcggct catcacatcc tggggctgta gtcggtccca
     4321 agggtatggc tgttcgccat ttaaagtggt acgtgagctg ggtttaaaac gtcgtgagac
     4381 agtttggtcc ctatctgcag tgggcgttgg aagtttgacg ggggctgctc ctagtacgag
     4441 aggaccggag tggacgaacc tctggtgtac cggttgtaac gccagttgca tagccgggta
     4501 gctaagttcg gaagagataa gcgctgaaag catctaagcg cgaaactcgc ctgaagatga
     4561 gacttccctt gcggtttaac cgcactaaag ggtcgttcga gaccaggacg ttgataggtg
     4621 gggtgtggaa gcgcggtaac gcgtgaagct aacccatact aattgcccgt gaggcttgac
     4681 tctatcattt gaaggacttc aagagataaa agcttactga ctgattcagt cattaccgaa
     4741 tatattgatt aaggctttac cgatttgcaa cagtttaagt ttggcggcca tagcgagttg
     4801 gtcccacgcc ttcccatccc gaacaggacc gtgaaacgac tcagcgccga tgatagtgtg
     4861 gttcttccat gcgaaagtag gtcactgcca aacacccttt cagaaaaccc ccggatatcc
     4921 gggggttttt gctttgcccg gaaaaaatgt cggggatggc gggacggcat ctgtacggtg
     4981 tccggtcggg tttgcggagg aacggcttga aactttggga tattcatttt agaatgaccc
     5041 gttttatagc ggattaacaa aaatcaggac aaggcggcgg gccgcaggca gtacggatag
     5101 tacggaaccg gttcgcccgg tgcttcatca ccttagggaa ccgttccctt tgagccgggg
     5161 cggggcaacg ccgtaccggt ttttgttaat ccgccatatc gtcgcaagat gcggtttgtt
     5221 gtttgcaacc cttaaaggaa aaaccatgaa aaaaatgttt gtattgttct gtatgctgtt
     5281 ctcctgcgcc ttctcccttg cggcggtaaa catcaatgcg gcttcgcagc aggagctgga
     5341 ggcgctgccg ggcataggcc cggcgaaggc gaaggccatt gcggaatacc gcgcgcaaaa
     5401 cggcgcgttc aagtctgtgg acgatttgat caaggtgaag ggcatcggtc cggcggtgct
     5461 ggcgaagctg aaagaccagg cttccgtcgg cgcgcccgca ccaaaaggcc ccgccaaacc
     5521 ggtgctgcct gcggttaaaa aataggggaa c
//
LOCUS       DBJGGI010000095         5383 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.095, whole genome shotgun sequence.
ACCESSION   DBJGGI010000095 DBJGGI010000000
VERSION     DBJGGI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.095"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            444..662
                     /locus_tag="ACSIGJ_001951"
     CDS             444..662
                     /locus_tag="ACSIGJ_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330747.1"
                     /translation="MANSNSGHSKKLRAATAAAATKAKLASGEYRQFSVQGRAEDVEL
                     ILAAVEKAGGSRVQALAKICRRYLEGLS"
     gene            complement(679..1038)
                     /locus_tag="ACSIGJ_001952"
     CDS             complement(679..1038)
                     /locus_tag="ACSIGJ_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330748.1"
                     /translation="MQVLLGEDFKRALKNYPKEDRRKIAEFIAHVQQNGLSGLPGRNK
                     SSDNVPADDPQWLEKVRFAQRHNLWHYHIGIPKYNGGRYGDLTSAYILHYTLCDGFIK
                     IIGFDRHPPFILPDIPK"
     gene            complement(1039..1578)
                     /locus_tag="ACSIGJ_001953"
     CDS             complement(1039..1578)
                     /locus_tag="ACSIGJ_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HHQ1330749.1"
                     /translation="MLNAYDVADFFLSPFEEEDGEQISNLKLQKLLYYAQGYALAILN
                     RPLFAENIEHWQHGPVVPCIYRTYKKYGGSPLPAAHIEPDKYADEELVVLNRVRKEQG
                     CYTAWALRNKTHQEAPWIQTRQGEVIGIALMGEYFRHALPQTDYNFNLEKLKTAVEDS
                     FVSVPHFNGADDLEKWLEQ"
     gene            complement(1738..2442)
                     /locus_tag="ACSIGJ_001954"
     CDS             complement(1738..2442)
                     /locus_tag="ACSIGJ_001954"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HHQ1330750.1"
                     /translation="MTNRDLKAIRIDNLKRFFENKKLPVKDKSLLSQLMSGKASFGEK
                     VSRRLEEEYGMGSLYLDSVPSEPTPVLINPDLPHEVKDIHRPMMWSSNDPLPDDDYVF
                     VPYLKESCFKGGAGAYEIPDYNGYRLPFGKSTLRRKGINPDNVFCCTLTGDSMEEKIA
                     EDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYPDETAPL
                     DSLSVIGRVFWWSVLD"
     gene            2570..2785
                     /locus_tag="ACSIGJ_001955"
     CDS             2570..2785
                     /locus_tag="ACSIGJ_001955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330751.1"
                     /translation="MTHSEFIKTLSEQRGAKTEYAKKLGLSLSFLWQIENGRAVTPKR
                     LYKDVMLLTKNKVSISELISEFRVCPK"
     gene            2865..3020
                     /locus_tag="ACSIGJ_001956"
     CDS             2865..3020
                     /locus_tag="ACSIGJ_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330752.1"
                     /translation="MKKQDRNRLSKKDRRLIKKAMLKAAAKGCDEVYKIAPGLKDGFE
                     LLGKQPD"
     gene            complement(2997..3185)
                     /locus_tag="ACSIGJ_001957"
     CDS             complement(2997..3185)
                     /locus_tag="ACSIGJ_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330753.1"
                     /translation="MPSDLSFILAKELIRSGSIRLSGSTAKGQAGELAVFIRTLHQKP
                     EESEPNTDDEYLIGLLSK"
     gene            3358..3585
                     /locus_tag="ACSIGJ_001958"
     CDS             3358..3585
                     /locus_tag="ACSIGJ_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHQ1330754.1"
                     /translation="MNQKQTQCKQIVDYIRNKGCITSLEAYQNLKVTQLAARITDLEG
                     KGFVFAKPRMKAGGRGKPVTHYSIVKNGAEV"
     gene            3582..4592
                     /locus_tag="ACSIGJ_001959"
     CDS             3582..4592
                     /locus_tag="ACSIGJ_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHQ1330755.1"
                     /translation="MSARLMGMAFKTGIPRGQRFVLVKLCDCANDEGLCYPSQETLAE
                     DTGFAETAVRQHIKWLKDNNFIKSARRQRGRERKSDIYRINVALLEKCYAEAAKRKAA
                     RQAKMWEEPLDYEPSDYEPSDFEPSDYEPSDYEPSDFDAKNHQILSGEPSDFALRTVR
                     FCAKNHQILSDEPSDFDGSLYVEPSVEPSVEPSGSNARGARAPAGPHPAKPQTAPPET
                     APAAKAKKTGRHETELSLLADYGITGQVAADFLQVRKAKRQPLTETAMRLIAADAEKC
                     GMTALQAAEYAIASGWGSFRADWLQNKTFGRSGNRGGPTHNQTAAVPDAGSYGDMPTT
                     DF"
     gene            4609..>5383
                     /locus_tag="ACSIGJ_001960"
     CDS             4609..>5383
                     /locus_tag="ACSIGJ_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHQ1330756.1"
                     /translation="MALRNASDFLGAYGGGVRVERRQCAEHGGYAAKSVLRGVWTGCP
                     ACRKLEAADEMAAYAETLRRGAMRDALEKRIGRSGIAPRFRNCRIENYAVSDSIPGMA
                     RAKAAAAEYAANFADVLQTGRSMIFSGRRGTGKNHLACGIAREVIAAGKSALVITVGD
                     MLRTVKDSFGGGGGEAGAVGVFVKPDLLVLDEFGAGSLSETDGRILFSVVNARYERLM
                     PMLVLTNLTAEAFRENTDARIRDRLRDGGGKLIPFDWESY"
BASE COUNT         1290 a         1267 c         1576 g         1250 t
ORIGIN      
        1 tttagtaaac acttaacatc tagtcaagta aaatgtttag tgattattta ataaatactg
       61 aacattttga ttattaaaag aatttatttt tgagatttcg caggcgcaaa aaaaaccgcc
      121 tattaaggcg gttgtttagg aaagaatgcc ctctgcgcgt gtagggtatt tcgatacttg
      181 cgtttattat atcatatgat ataataaact catagattag gaaatgaaga aaccccgtat
      241 cagccgatac ggggcaacct gaaagagaaa ggaggcaatg tgattaagaa aatcctaatc
      301 tgtgtgattt tgtatttcct tgcaagctcc ccagcgtggt agggaaatac aaaacaagtg
      361 attaacgatt aaccatttgc agggcagtta aggaggctgc ccttcctctt atctttccca
      421 tactctacta taaggattta aaaatggcaa attccaacag cgggcattct aaaaaattgc
      481 gcgcggcaac ggcggcggcg gcgacaaagg caaaactggc aagcggcgaa taccggcagt
      541 tttcagtgca ggggcgtgcc gaagacgtgg agcttatcct tgccgccgtg gaaaaagccg
      601 ggggcagccg cgtacaggct ttggcaaaga tttgcaggcg gtatctcgag gggctgtctt
      661 aaggggggta agacaaggtt atttgggaat atcgggcagg ataaaaggcg ggtgtctgtc
      721 aaaaccgatg attttgataa agccgtcgca aagggtatag tgaaggatgt aggcggatgt
      781 caaatcgcca tacctgccgc cgttgtattt gggaatgccg atgtgatagt gccaaaggtt
      841 gtgccgttgg gcaaatcgga ctttttccag ccattgagga tcgtctgccg gtacgttgtc
      901 gctggatttg ttcctgccgg gtaaccctga taagccgttc tgttggacgt gtgcgataaa
      961 ttccgcaatt tttctgcggt cttctttggg gtagtttttc aacgctcttt tgaagtcttc
     1021 gccaagcagc acctgcattt actgctccaa ccatttttcc aagtcgtcgg cgccgttgaa
     1081 atgcgggacg ctgacaaagc tgtcttcaac ggctgttttg agtttttcaa gattgaaatt
     1141 gtaatccgtc tgcggcagcg catggcggaa atattccccc atcagcgcaa tccctatgac
     1201 ttcgccctgc cgggtctgta tccacggcgc ttcctgatgg gttttattgc gtaatgccca
     1261 agcggtgtag cagccctgct ctttacggac acggttgagg acaaccaact cttcgtccgc
     1321 atatttgtcc ggttcgatat gggccgcagg caatgggctg ccgccgtatt ttttgtaggt
     1381 gcggtaaatg caggggacta ccggaccgtg ctgccagtgt tcgatatttt cggcaaacag
     1441 ggggcggtta agtatggcaa gggcgtagcc ttgtgcgtaa tacaggagtt tttgaagttt
     1501 gagattggag atttgctccc cgtcctcttc ttcaaaaggg gaaaggaaga aatctgccac
     1561 gtcgtacgcg ttcagcattt tctgtttccg ttgttatttt tctgtatatt aaggccgccg
     1621 gcccattatt tcaagaaata atttgtgcaa attatccggt atcggcaggc tgtccgcgcc
     1681 gtcggcatat cgggcggata tatttgaaaa atacttgccc tgccgtttca gacggcatca
     1741 atccaacacg ctccaccaaa agaccctgcc gataacggac aggctgtcca aaggggcggt
     1801 ttcgtcggga tagaagccgc tgttgtggct gcgtatcagc acgctgttgc cgggctgccg
     1861 tatcaggtat ttgacgcgga acatgccgtc ttgggcgaag gcgtatattt tgccgtcgcg
     1921 tatggcggtt tcgcccgtat ctacggcgat tgccgcgtct tctgcgattt tttcctccat
     1981 gctgtcgccg gtcagggtgc agcagaagac gttgtcgggg ttgatgcctt tgcgccttaa
     2041 cgttgatttg ccgaacggca ggcggtagcc gttgtagtcg gggatttcat acgcgcccgc
     2101 cccgcctttg aagcagctct ctttgaggta ggggacgaaa acataatcat cgtcgggcag
     2161 cgggtcgttg ctgctccaca tcatcgggcg gtggatgtct ttgacttcgt ggggcaggtc
     2221 ggggttgatg aggacgggcg ttggttcgct cggaacggaa tccaagtaca gactgcccat
     2281 cccatattct tcttcaagcc gtctgcttac cttttcgcca aaagatgctt taccgctcat
     2341 caattgagat aaaaggcttt tgtctttaac cggcaacttt ttattttcaa agaatctttt
     2401 taaattatcg atacggatgg ccttcagatc tctattagtc attttcgcgc tccattcaga
     2461 ttgggattca attttattta gtttctgcta aacaaacaaa tattaaattt tcttgcctat
     2521 gcgtttaata tttgctaaac tcattaaata tttacggtga aactacagaa tgactcattc
     2581 agaatttatt aaaacgctgt cagaacagcg tggtgcaaaa acggaatacg ctaaaaaact
     2641 tggcttatcc ctgtcttttc tatggcagat agaaaacgga cgggcagtaa cgccaaagcg
     2701 tctttataaa gacgttatgt tgttgacaaa aaataaggtt tctatttctg aattaatttc
     2761 ggagttcagg gtttgtccca aataaaaagc ccgtcgggtc agatgttaat tgatataacc
     2821 gaattgaagc ggaagtcatc cgcaatttac cggaaaggaa aaaaatgaag aagcaggaca
     2881 gaaaccgcct gtcgaagaaa gacagacggc tgattaaaaa ggcgatgctg aaagccgccg
     2941 ccaaaggctg cgatgaggtt tacaaaatcg cgccgggttt gaaagacggc tttgaattac
     3001 ttggaaagca gcccgattaa atattcgtca tcggtatttg gctccgattc ttcgggtttt
     3061 tgatgaagtg ttcggatgaa taccgccaat tctccggctt gtcctttggc cgtactgccg
     3121 cttaagcgga tagaaccgct gcggataagc tctttggcga gtatgaagga taagtcggac
     3181 ggcatttttt tactccgtcg gccgttgtgt ggaaacccgg ttgcaacggg gtgatggcaa
     3241 atcggaaaga cggctgaccg cccggacaga cgggcggccg ataaagaaaa acccgcacgg
     3301 ggcgggtaat ccccctgaat tgcagggaag cggttcaggt aacggcgaaa ggcgattatg
     3361 aatcaaaaac aaacgcaatg caaacaaatt gtcgattaca tccgtaacaa gggatgcatc
     3421 acatcccttg aggcttatca gaacctgaag gtgacgcagc ttgcggcacg gataaccgac
     3481 ttggaaggca agggcttcgt gtttgccaag ccgcgcatga aggcgggcgg ccgcgggaag
     3541 cctgttacgc attattcgat tgtcaaaaac ggagcggaag tatgagtgcg aggctgatgg
     3601 ggatggcttt caaaacgggt atcccgaggg ggcagcgttt tgttttggtg aagttgtgcg
     3661 actgcgccaa cgacgagggc ttgtgttatc cgtcgcaaga aacgctggcg gaagatacgg
     3721 gcttcgccga aaccgccgta cggcagcata tcaagtggct gaaggataac aatttcatca
     3781 agtccgcccg gcggcagagg gggcgggaga ggaagtccga catctaccgc atcaacgtcg
     3841 ccctgcttga aaaatgctat gcggaggcgg caaaacggaa ggcggcgcgg caggcaaaaa
     3901 tgtgggaaga accattggat tacgaaccgt cggattacga accgtcggat tttgaacctt
     3961 cggattacga accgtcggat tacgaaccgt cggattttga cgctaagaac catcagattt
     4021 tgagcggcga accgtcagat tttgcgctaa gaaccgtcag attttgcgct aagaaccatc
     4081 agattttgag cgatgaacca tcagattttg acggttcctt atatgtagaa ccgtcagtag
     4141 aaccgtcagt agaaccgtca ggatcaaatg cgcgcggcgc gcgcgcccct gccggaccgc
     4201 accctgcgaa accgcaaacg gcgcctcccg aaaccgcacc ggcggcgaag gcgaaaaaaa
     4261 ccggcaggca cgaaaccgag ctttcgctgc ttgccgacta cggcatcacg gggcaggtgg
     4321 cggcggactt cctgcaagtc cgcaaggcaa aacggcagcc gctgacggaa acggcaatgc
     4381 gcctgattgc cgccgatgcg gagaaatgcg ggatgacggc gctgcaggcg gcggagtacg
     4441 ccatcgccag cggctggggc agcttccgcg ccgactggct gcaaaacaaa actttcggca
     4501 ggtccggaaa ccgcggcggc ccgacgcaca accaaaccgc cgccgtgccg gatgcgggaa
     4561 gctacggcga tatgccgacg acggattttt gagggggggg gttcggatat ggctttgagg
     4621 aacgcgtctg atttcttggg ggcttacggc ggcggcgtgc gggtcgagcg gaggcaatgc
     4681 gcggaacacg gcggatacgc ggcgaaaagc gttttgcgcg gcgtgtggac gggctgcccg
     4741 gcctgccgga agctggaggc ggcggacgaa atggcggcat acgcggaaac gctgcgccgc
     4801 ggggcgatgc gcgacgcgct ggaaaaacgc atcgggcgtt cgggcatcgc cccgcggttc
     4861 agaaactgcc ggattgaaaa ctacgccgtc agcgattcga tcccggggat ggccagggcg
     4921 aaggcggccg ccgccgagta tgcggcaaac ttcgccgatg tgttgcagac ggggcggagc
     4981 atgatttttt cgggcaggag gggcacgggc aaaaaccacc ttgcctgcgg catcgcccgc
     5041 gaagtcatcg ccgccggcaa aagcgcgctg gtcatcacgg tgggcgatat gctgcggacg
     5101 gtcaaggaca gtttcggcgg cggcggcggc gaggcggggg cggtcggggt tttcgtgaag
     5161 cccgatttgc tggtgctgga cgagttcggc gcgggcagtc tgtcggaaac ggacgggcgg
     5221 attttgtttt ccgtcgtcaa cgcccggtac gagcggctga tgccgatgct ggtgctgacc
     5281 aacctgacgg cggaagcctt ccgcgaaaac accgacgcgc ggatcaggga caggctgcgg
     5341 gacggcggcg gcaagctgat tccgttcgac tgggagagct acc
//
LOCUS       DBJGGI010000096         5245 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.096, whole genome shotgun sequence.
ACCESSION   DBJGGI010000096 DBJGGI010000000
VERSION     DBJGGI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.096"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(422..1264)
                     /locus_tag="ACSIGJ_001961"
     CDS             complement(422..1264)
                     /locus_tag="ACSIGJ_001961"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHQ1330757.1"
                     /translation="MLICNPYEVVIHGTTSSGKIFRPGDWAERLCGILSSFTKDNRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNDPANERVLLAQAIEEKHAAEKTQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDIGRFTEQINKTQRPQGYRLLGIFEEGKHNAVAVCGFREACTLAGGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKISAETGVTKIHLNVHVNHDRTDAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            1499..2419
                     /gene="pyrB"
                     /locus_tag="ACSIGJ_001962"
     CDS             1499..2419
                     /gene="pyrB"
                     /locus_tag="ACSIGJ_001962"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HHQ1330758.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     VQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELEEADCRYRALGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLARAKPNLRVLHPLPRTDEIHPDVD
                     AAPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            2429..2887
                     /gene="pyrI"
                     /locus_tag="ACSIGJ_001963"
     CDS             2429..2887
                     /gene="pyrI"
                     /locus_tag="ACSIGJ_001963"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HHQ1330759.1"
                     /translation="MEAQKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLTLPDEI
                     AEVFRCPNPNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYNRDSVAEA"
     gene            2949..3614
                     /locus_tag="ACSIGJ_001964"
                     /pseudo
     CDS             2949..3614
                     /locus_tag="ACSIGJ_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4019..5242)
                     /locus_tag="ACSIGJ_001965"
     CDS             complement(4019..5242)
                     /locus_tag="ACSIGJ_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HHQ1330760.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPDAPQRYTVKQGDTLW
                     DISGKYLYSPWQWGSLWGANRDQIHNPDLIYPGQVLVLRYVGGEPRLDLEQTDGIPVV
                     KMSPDKEVSGYGIPAVNANFYRIFIRHPQIVSRKETAAAPRLLSGPEGRLLYTKGARV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSALEQRSKQAEE
                     RLKDNEYYTRTHPLITPVRTRSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMVPHEP
                     SRPVQAKIVSVFEGVGVGGQFKTITIDKGGDDGLDKGAVLSLYKRKKTMQVNLSNNLT
                     EEPKSRDTVELISTPAEEVGLAMVYHTAPKLAYAIILENISDISEGDTAANPGRDLDN
                     MPDQGRARVDSDPFQ"
BASE COUNT         1135 a         1352 c         1584 g         1174 t
ORIGIN      
        1 gacaaggcga cgaagccgca gacagtacaa atagtacgaa accgattcac ttggtgcttc
       61 agcaccttag ggaatcgttc tctttgagct aaggcggggc aacgccgtac tggtttttgt
      121 taatccacta taaatagcga gcatcgattc acggtgcgct ttagtgcaaa gcgtacaacg
      181 cgagcctgaa ccaccagccg caataggaaa agaaagccga tagagtgcaa tgcttgccaa
      241 cgcgcaagcg agcttgcaag aacgcttggc tcaacgagag caggcaagac agaaagcagg
      301 ataggagagg caacgcaaag gtctcgggct ttgatttcgc cgtaaacctg agtgccgcct
      361 tgtccggacg gggtggttca ggcggcgagt gccgacaggg tgcagatgag gaggagtatt
      421 ttcatttggg gtcgcaacgg aagtggtagg cgcagatttc aaaaccgttt ttgaaataca
      481 ggcggtgcgc gtcggtacgg tcgtggttga cgtggacgtt aaggtggatt ttggtaaccc
      541 ctgtttccgc gctgattttg cggacttctt ccaaaaggcg cgaggcgtag cctttgcggc
      601 ggctttgcgg cagggtaacg atgtcgtcga tgtggatgtg gcggccgccg gcgagggtgc
      661 aggcttcgcg gaagccgcag acagcgacgg cgttgtgttt gccttcttca aaaatgccca
      721 gcaggcggta gccttggggg cgttgggttt tgttgatctg ttcggtaaag cggccgatgt
      781 cggtcagggc ggaacgcaaa acactcaagg ctgcaaaggc ggtggcggtg tcgtccgcgc
      841 cgatttcgcg taaaacgtag gatgcgcccg aggcggtctg ttcctgtgtt ttctcggcgg
      901 cgtgtttttc ttcgattgcc tgtgccagca ggacgcgttc gttggcaggg tcgttttgtc
      961 cgccctgttc gcgttcttcg agcagggctt tgcagtcgat gacgcgcagg tcgttgtcgg
     1021 cggcaaagtc catcaggaag cggaacatct gcggattgtc ggtttccagt tttttatcga
     1081 cggcgacgca gcggatgttg tccaccagca tggggcgcac ccatttcgaa taggagaggc
     1141 ggttgtcttt ggtgaacgag gacaggatgc cgcacaggcg ttccgcccag tcgccggggc
     1201 ggaaaatctt gccggaactc gttgtgccgt ggatgacgac ttcgtagggg ttgcagatta
     1261 acatggcggc ttcctgaaaa gaaatgtctg gcgcgattat accttatgct tatgcgggcg
     1321 tgtttggata tgccgtctga aaaatacagg attcgtgcgg taaaactttg tggaggcaaa
     1381 tgtgcgataa tacgcgccgt gtcgccgctt ttgcgaagct gttccgcaaa catacgggcg
     1441 gcgtggacgt ataaccggat gcccgcctga cgcgggtttt ttacggaagg ggggcaaaat
     1501 gcctaatccg ctttacagac agcacatcat ctccatttcg gatttgtcgc gcgaacagtt
     1561 ggaatgcctg cttcagacgg cattgaagct gaaggcgcat ccgcgcggcg atttgttgga
     1621 aggcaaactc atcggttcgt gctttttcga gccgtccacg cgcacgaggc tgtcgtttga
     1681 aacggcggtg cagcgtttgg gcggtaaggt catcggcttt tcggacggcg cgaataccag
     1741 tgccaaaaaa ggcgaaacgc ttgccgatac cgcccgtatc atttccggct acaccgacgc
     1801 gattgtccaa cgccatccca aagacggcgc ggcgcgcgtg gcggcggagt tttcgcgcgt
     1861 ccccgtcatc aacgccggcg acggcacgaa ccagcacccc agccaaacct tgttggacct
     1921 ggtaacgatt tatgaaacgc aggggcgttt ggacaagctt aaaatcgcca tggcgggcga
     1981 cttgaaatac gggcgcaccg tgcattcgct gtgtcaggcg ttgaaacgct ggggctgcga
     2041 atttgccttc gtctcgccgc ccagcctcgc gatgcccgac tacattaccg aagagttgga
     2101 agaagccgac tgccgatacc gcgccctcgg cagtttggaa gaggcggcgg aatgggcgga
     2161 catcctgtat atgacccgcg tccagcgcga acgtttcgac gaacaggaat ttgccaaaat
     2221 ccaaggcaaa ttcaacctcg aagcgtctat gctcgcccgc gccaaaccga acctgcgcgt
     2281 gctgcacccc ctgccgcgca cggacgaaat ccaccccgat gtcgatgccg cgccgcacgc
     2341 ctattatttc gagcaggcga ccaacggcgt ttatgcgcgt atggcgatat tgtcgctggt
     2401 gttgaacgaa gaagtgtgag gaaccgatat ggaagcgcaa aaactcagtg tcgaagccat
     2461 cgaaaaaggc acggttatcg accatattcc cgccggcagg gggctgacca tcctgcgcca
     2521 gttcaaactt ttgcactacg gcaacgcggt aaccgtgggc ttcaacctgc ccagcaaaac
     2581 ccagggcagc aaagacatca tcaaaatcaa aggcgtgtgc ttggacgata aagccgccga
     2641 ccgcctcgcc ctgttcgccc ccgaagcggt ggtcaacacc atcgacaatt tcaaggtcgt
     2701 acagaagcgg catttgaccc tgcccgacga aatcgccgaa gtgttccgct gcccgaatcc
     2761 caattgcgcc ggccacggcg agccggtcaa aagccggttt tatgttaaga aacacaacgg
     2821 gcagacgcgg ctgaaatgcc actactgcga aaaaacctac aaccgggatt cggtggcgga
     2881 agcctgacgg attcccgagc ccggcggcgg aattttgcct gccgcctgtt ttgccaatct
     2941 gaaatggaat gatgatgcac gcttctgtcc aaagtcgttt cgcaccgata ctttatgttt
     3001 tgattttctt tgccggtttt ttgaccgcgc aaatctggtt caatcagaaa gcctatactg
     3061 aagagctgcc tccgcttctg tccgcattgt ccgccgtcgc gctggtgtgg ctggcgtggg
     3121 cgttcgtgtc ggtgcgttca aaggctaagg cagaaaagtt ctaccgcgaa aaaatgatac
     3181 agaacgaaag catacccccg tcctgcacgc ttctttgcaa cacttggaac acaagccgca
     3241 aatgctcgcc ctgctggtca aaaaccacgg caaaggcatg gcggaacagg tcaggttcaa
     3301 ggcggaagtg ctgcccgacg acgaagacgc gcgcacgatt gccgccgagt tggcaaaaat
     3361 ggatatgttc gcattgggga cggacgcggt cgcctcgggc gaaacctatg ggcgcgtgtt
     3421 cgccgatatt ttcgagttgt cggcggcttt ggaagggcgc gcgttcaaag ggatactgaa
     3481 actgacggcg gaatataaaa aacatcttcg gcgatgcctg ccgttcggaa acggcgttgg
     3541 atttgggcgc gctcaatcag gcgttgaggg aaatctcgaa aacgccggaa aagcctaagc
     3601 agatatttta ttgaaggtgt cccgatgtat ggggagcgca cgggtggcgc ggcggcaaag
     3661 cggcaagatt gggtttggat acccggttgg ttttatagta gattaacaaa aatcaggaca
     3721 aggcggcggg ccgcagacag tacaaatagt acggaaccga ttcgcccggt gcttggacac
     3781 cttagggaat cgttcccttt gagccggggc ggggcaacgc cgtaccggtt tttgttaatt
     3841 cgctatatct gtctgcaagg gagacagccg ctgtgatttc cgttcgggcg gaaaccgatt
     3901 ccatcggata aaaggcattt tgtccgacta aatcagggtt ttgcatcaac gggatatgaa
     3961 aacacagcct aaataaaaat gccgtctgaa tgctccagac ggcattgttg ccgaggggtt
     4021 attggaaagg gtcggaatcg acgcgggcgc ggccctgatc cggcatattg tccaaatccc
     4081 gtcccggatt ggcggcggtg tcgccttcgg aaatatcgga gatgttttcc aaaataatgg
     4141 cgtaagccag tttcggcgca gtgtggtaaa ccattgccaa gcccacttct tcggcaggtg
     4201 tcgaaatcag ctcgacggta tccctgcttt taggttcttc ggtcaaattg ttggagaggt
     4261 tgacctgcat cgttttcttg cgtttgtaaa ggcttaaaac tgcgcctttg tccaaaccgt
     4321 cgtccccgcc tttgtctatc gtaatggttt tgaactgtcc gccgaccccc acgccttcaa
     4381 acacggaaac gatttttgcc tgaaccgggc gggacggttc gtgcggcacc atattgaagc
     4441 ggtcggtgtc ttccggcatt ttcatcaggt agtcgccctg ctgtatttcg gaaatggcgg
     4501 tttcgaccac cagcggctgt atcgaacggg tgcgcaccgg ggtaatcaaa ggatgggtgc
     4561 gggtgtagta ttcgttgtct ttcagccgtt cttcagcctg tttcgagcgt tgttcgaggg
     4621 cggagtcggt atagtcgagg gagcgcacga tgccgctgaa tgcgacttct tgtccgagga
     4681 atttacccgt atccggatcg gtgatgtttt tattgatgcg gtaggtcagg tagcggcccg
     4741 gctctttcag gcctttggtg taaaccctgg cgcctttggt gtacagcagc ctgccttccg
     4801 ggcccgagag caggcgcggc gcggcagcgg tttctttgcg ggaaacgatt tgcgggtgcc
     4861 gtataaagat gcggtagaag ttggcattga cggcagggat gccgtatccg gacacttctt
     4921 tatccggact cattttgacg acggggatgc cgtctgtctg ttccaaatca aggcgcggtt
     4981 cgccgccaac gtagcgcaac accaatacct ggcccggata aatcaggtcg ggattgtgga
     5041 tttgatcccg gttcgcgccc cacaggctgc cccattgcca cgggctgtac aggtatttgc
     5101 cggagatgtc ccacagggtg tcgccctgtt tgaccgtgta gcgttgcggc gcatccgggc
     5161 gcacctccag atttgccgcc aaagtttgtg ttgagaatgc catgcctgcc gcgcagagca
     5221 gggttataat acgacgttgc ataac
//
LOCUS       DBJGGI010000097         5238 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.097, whole genome shotgun sequence.
ACCESSION   DBJGGI010000097 DBJGGI010000000
VERSION     DBJGGI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.097"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(228..3427)
                     /gene="nalP"
                     /locus_tag="ACSIGJ_001966"
                     /pseudo
     CDS             complement(228..3427)
                     /gene="nalP"
                     /locus_tag="ACSIGJ_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
     gene            complement(3537..3913)
                     /locus_tag="ACSIGJ_001967"
                     /pseudo
     CDS             complement(3537..3913)
                     /locus_tag="ACSIGJ_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4065..4388)
                     /locus_tag="ACSIGJ_001968"
     CDS             complement(4065..4388)
                     /locus_tag="ACSIGJ_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330761.1"
                     /translation="MFLDDVNVFLDDLNTNPITDEWYMSNFADKHIKILESYEAFDIL
                     KQFVDYMIEEHDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQEISQDIL
                     NEIFR"
     gene            complement(4391..4579)
                     /locus_tag="ACSIGJ_001969"
     CDS             complement(4391..4579)
                     /locus_tag="ACSIGJ_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HHQ1330762.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNINKVEI
                     HYIENTRTGEKTDFKFKD"
     gene            complement(4647..4985)
                     /locus_tag="ACSIGJ_001970"
                     /pseudo
     CDS             complement(4647..4985)
                     /locus_tag="ACSIGJ_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(5055..>5238)
                     /locus_tag="ACSIGJ_001971"
     CDS             complement(5055..>5238)
                     /locus_tag="ACSIGJ_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330763.1"
                     /translation="NFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGI
                     IIKISAETINFEPLGK"
BASE COUNT         1109 a         1292 c         1246 g         1591 t
ORIGIN      
        1 ggttccctaa ggtgctgaag caccgggcgg atcggttccg taccatccgt actgcctgcg
       61 gcccgccgcc ttgtcctgat ttttgttaat ccgctatacg tctgattgat gccgaatctt
      121 tggaagaagt cttgaaacaa tagaagcagg caattggaat agggttttct tttcataaga
      181 aacagccgca aagaccgtga tctttgcggc tgtctgtttt ctgtccgtca gaaccggtag
      241 cctacgccga tttgtccgct gtggttgccg tactgtttgg aaccggtgta gctgtaacgt
      301 gccaagccgt tccagccgtt gccgaattcg acatccaccc ccagaccggc aacccggcgg
      361 gtgtgcggca tattgcgtgc acccgtcttg ccggttgctg cagccgcgcc ggtaaagccg
      421 cccgttaccg cgtagtcgcg tccgttcagg tcgcgttcca cgcccgccgt cgcagacagg
      481 acggctttat cgctcaaggg ttgcgacagc ttcagacccg cgagtccgac cagtgtgcct
      541 tcagtgaggc tgttgccgct ccagcccaaa gcactgcctt tttcggcgaa tgcatcctgt
      601 ttgagcaggt cgtggcgcag accgccttca accgtcaaat ctcccgttgc ggcaaacgga
      661 acgttgacac cacccagtgc gcccagctgc atcagcgtgc cgttgacgct gccttccgca
      721 tattcatccg caccggtgct gcggctgatg ctgtttttgt agcgtccgta ggagaacagg
      781 cctttgagat agccgatatc gcccacatcg tgccgtatgc ctgcaaacag actaatgctg
      841 tcggtttttg catttgcact gttttcgctc catgtgctgc gtcctatgcc cagtgtggcg
      901 gctgctgtcg tattttcgcc ggttttcgcg gcaatgccga tagtttgggt actgccgcgc
      961 attttgcctt cgacaccgcc ctgttcccac gttccaccgt cctgttgggt ttgcgcgatg
     1021 acgcgcagac ccgtaccgtt gtggtccaac ccgtccgata cggctttcag gcggcgtccc
     1081 tgcatatcgg catgggcggc ggcactgtcg gcatagacgg tagcggcgag actgttgaag
     1141 atgcgtacgc cgtcggcggt attcgcatgc tgtacggctg ccgctgcgcg gaaagttgtg
     1201 cgccgtaggc ggatgcccgg catatctgtg cggtcggcga ccgcagtttc aaccgtctcg
     1261 ggtgttgcgg atgattcgga ggcatccagc tcgaccatca ggttttccag attgctgccg
     1321 ccctgttcta cggcgtgttt cagaccggcg ggcgcggaat gtgccgctgc cgaagcagtc
     1381 cgtgccgcat tgccgcgacg gacataatag gacggcgtgt cgccttcact gcccgctgtt
     1441 ttttcgacgc tgtcgaggga agccagcaga ccgccgtcgg tttcgatatt tttgaagaaa
     1501 gaataatcct gcccgatttt ggcggcactc aggaagggaa cacgtcgtcc ggtactgttg
     1561 agatagcctg cccccttgcc gcgtgccgac atgtacagct tgccgtcggt aatcgccgta
     1621 ccgtccactt tcagcagttt gcccaaacgt gtgtacagcg tacctttgtc gcccagctgc
     1681 agaccgcctt ttgatgtgta cggtttcgtt tgcgccggat tggtcggtat ctgccagata
     1741 gacaatgccg tggctgttca ggctgccgcc ggatgccgcc ccgttataaa tcagtgcacc
     1801 tttggttttg acgtgcatat ccgataacac cagagaaccg ccttcgataa cggttttgcc
     1861 cgtataggtg ttgttgccgt gcagttgcag ttggctgccg ccttttttga tcagaccgcc
     1921 cgtgcctgaa atgtcgttac ggaaggagta ggcaatatcg gatgtacctt tcgtatcggc
     1981 ggtaaagtcg ccgaacggaa aggacgcggg tccgttcatg gccttacccg catccagcag
     2041 cccccagccg aacttgctgt ccactccgac tgcaccgatg tcctgagccg tcgtcagcag
     2101 cgtggtacgc aggttgtcgt tgctcatcca cgggtatctc tgcagcagag ccgccgtgcc
     2161 ggttacgatg ggtgcggaaa aggatgttcc ggcaacttga atcggtcttg tacgggtgaa
     2221 atggacggtt gcttcatagg atgccgacag gcgccacatg gcagtaattc cgcaatggtt
     2281 ggagccattg aacttttctc cactgcggtc tacgcctgcg actgtgataa tgcctttttg
     2341 agcgtctttt cataaaatgg caatagggtc agtgtgttgg gctgagcttg tgtgtcattg
     2401 cctgtcgaaa agatgaaaag gatgttttta ttacggatgt ggtaggacag gttgccctaa
     2461 tcgctctgtt gcatcaggcg gataccctca tctgttttat caccgccgga atagtcgagc
     2521 aacgtttggc ggtattgctc ctccgaattg gctatttgga aatggtcggc agtgcctgcc
     2581 ctcgatgttg ttccaaaact gttattgacg atgcgcacgc cacgttcgcc cagcttgacc
     2641 catgcattgc ggatggctgc agacattatt tctcgttctt ggttccatca tgcgtattca
     2701 ttatgtgtag cgtcgcatcg ggcgcaatac cgcctgcagg tctgccgtcc acggaacgcc
     2761 cgccaataat atgggagacc acatcgatgt gtccgatttc ttttacatgg cggatatccg
     2821 tcggctttgc ttcagtctct ataacggcct cattgtccaa agaagcttca atgtctttac
     2881 cgcctctgtc ttcaggcgct tccttccgca tatacgccgt atagtttttg taatttgttt
     2941 ttgtaatttt cgttatagcc gtgttctttt ctgccataca gttcgggaaa ggatatgctg
     3001 ccgacggatt cgcctgtatc gacgatacct acctctaccc cgcgtcctgt atagcctgct
     3061 tcaattgcag gtttgaggtt gatcatattc ttaatattgg tcatttgggt ttgaaaagtc
     3121 tctggtatgc agattcgggg gctttgattt tggcatccct gtctgtaacc gcaacgtcat
     3181 cccgaccggc acagagcatg cttctgtctt tgcacatttc gttttttata ccggcgtaag
     3241 atactgctgc tgattccgct atcgttgccc tgctgttgct gccgataccg gtgccgcctg
     3301 cattaaagtc gggagcagaa gtgccgcctc cgccgccgcc taagcaggca gaaagtgttg
     3361 ttgcaacagc taacgccatg gcagccggtt tgaaagtttt tgtagggaag gttgaggtgg
     3421 ttcgcatgat gaggtctctt tgaatctgac aatagaattc aggcgaagga ataaaaattt
     3481 cgtaatgtcc agatgtaacc catttttata ttttgaatta tatctgtcct ggacaactag
     3541 gataaactcg attttactaa ttgttttaaa atggaacaag aacttttatc tcactgttgt
     3601 taaaacgccg ttcgcactcc tttaaatacg gctcaaaatg ctctttggga atgccgtcaa
     3661 acttgcgtaa atgacgtttt gcccggttcc aaaagttccc aattccatta atatggtttt
     3721 gtcgttcggc aaaatgtgtg ctgtgattga tacggaaatg gctaaattcg cccgcatcca
     3781 atacatcata gctacgataa caatccgtat aaacaatgct gtcaggctct gctgctgttc
     3841 ttgcagttac gcctgcgaca aacagttcaa tgagtttatt ttgtttatac cggcttagac
     3901 ggctttttct catagggata attctaactt aatttgaatt tccctagtta tctaggacag
     3961 ccccattatt tttatttcca gtcattctgg ctattttatg tatatttcca ttatgtttga
     4021 ttatcatttt attttttcca aggattttct tctatattga tagtttatct aaagatttca
     4081 tttaaaatat cctgactaat ttcttgttta tataattcta caattttctg tttctgtgta
     4141 tttgtataaa atttttcgtt ggtatctgct tgatatttta attgtctcaa tatttccatg
     4201 atttcatatt ctgatttttc atcatgttct tcaatcatgt aatcaacaaa ttgttttaga
     4261 atatcaaagg cttcgtaact ttccaaaatt ttaatatgtt tatcggcaaa attggacata
     4321 taccattcgt cagtgattgg attggtattt aaatcatcta aaaaaacatt tacatcatct
     4381 aaaaacatgg ctaatcctta aacttgaaat ctgttttctc accggttctt gtattttcaa
     4441 tgtaatgtat ctcaactttg tttatatttt gaacgcgctt aacccagccg tcttttgcca
     4501 accaaccatt tttagtatcg gacataggag gtattctcgc aggagttttg ccctgtgggt
     4561 ttgccttaac ttcttccatt gctaattgtt cctttaaatt attaggtatt gtacgaccgg
     4621 ttgatttttc aacagattgt gtccctattt ttaatgattt gtttcccccc agccatttcc
     4681 ctgtctacag tctgcatatg gcgggcagta ggtttttcat agccggtatc tttaactcaa
     4741 tcccatttag tcgggttaag ttctactttt ttacctctcc aaaccgtacc tgcggcctct
     4801 gctacggcaa gggcagtttg ggcagtagct gtaatgttcg gatgggcatc agcccattgt
     4861 ctggcagagt tgataccgtc ttttaccgca aaagcactgt cctgtaatag gctcacggcg
     4921 gcaagttgtg cttccggact taattttcct gaattattaa taccttgtag agtctgctgc
     4981 gcagagatgt agcaactgta tttttacccc gtcgggtaaa aatacagttg ctacggctgc
     5041 tgggaaagca gttactattt tcccagtggc tcaaaattaa ttgtttcagc agatattttt
     5101 ataataatcc ctacatcaat aataatttca ctataaaatt ggtatttata tagctctatt
     5161 tcgggtataa acaagggaaa caagctatct tccttatcct cataatctac gaaattattt
     5221 gtatctaatt taaaattc
//
LOCUS       DBJGGI010000098         5172 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.098, whole genome shotgun sequence.
ACCESSION   DBJGGI010000098 DBJGGI010000000
VERSION     DBJGGI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.098"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            103..924
                     /gene="ppsR"
                     /locus_tag="ACSIGJ_001972"
     CDS             103..924
                     /gene="ppsR"
                     /locus_tag="ACSIGJ_001972"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HHQ1330764.1"
                     /translation="MSSPRQVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATVSGQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYKDKLFGLTIQPERLQAIRQER
                     RPNSAYARIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(1010..1672)
                     /locus_tag="ACSIGJ_001973"
     CDS             complement(1010..1672)
                     /locus_tag="ACSIGJ_001973"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HHQ1330765.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTQLARHGLPEKSMDEIRTQ
                     ASHGAAGLLKLGANITPEHPDYTAWRTEYLEEYDSRYAQDTTLFDGVNELIAELGRRG
                     IKWGIITNKPMRFTDKLVPKLGFAVPPATVVSGDTCGEPKPSIKPMLHACGKIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYISDEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(1710..2537)
                     /locus_tag="ACSIGJ_001974"
     CDS             complement(1710..2537)
                     /locus_tag="ACSIGJ_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HHQ1330766.1"
                     /translation="MNPDIYALLERALLSGDPDEKGRLTDEAFAAVQNADGAEANAPP
                     ADFPRAGRPDTPVLVAPSQLTPRKMNTAEGYAAMLHAITHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLVRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVEQRGDSATCGVLDIIYRDEVGHVAIGNRWYQHLCRE
                     RGLEPVALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKHAV"
     gene            2920..3543
                     /gene="lolA"
                     /locus_tag="ACSIGJ_001975"
     CDS             2920..3543
                     /gene="lolA"
                     /locus_tag="ACSIGJ_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HHQ1330767.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTLPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVRATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            3816..4952
                     /locus_tag="ACSIGJ_001976"
     CDS             3816..4952
                     /locus_tag="ACSIGJ_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HHQ1330768.1"
                     /translation="MKKTLVAAILSLALTACGGGSDTAAQTPSAKPEAEQSGKLNIYN
                     WSDYVDPETVAAFEKETGIKMRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQIK
                     AGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKALG
                     TDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKAA
                     VDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVLT
                     PKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELMD
                     EKYTSDASIFPTKELMEKSFIVSPKSAESVKLGVKLWQGLKAGK"
     gene            5047..>5172
                     /locus_tag="ACSIGJ_001977"
     CDS             5047..>5172
                     /locus_tag="ACSIGJ_001977"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HHQ1330769.1"
                     /translation="MNLSIVVPIYNVESYLEACLNSIEPILSNENVELILVNDGSK"
BASE COUNT         1195 a         1411 c         1438 g         1128 t
ORIGIN      
        1 ttttttgggg tgtaatccga ggtaggggcg gctggggtgc ttctcccttg tctgccgctg
       61 ctgttatgat gatattttta ttctgtattt aaggaggggg tgatgagcag tccgcgccaa
      121 gtgttttata tttccgaccg caccggtctg actgccgaga atatcggcga ggctttgctg
      181 aaccagtttg gcaatctgtc gttcaaacgc catacgcatc cgtttgtcga tacgccggaa
      241 aaggcgcgcg cggtggtgga gaaggtcaat cggagccggc aggaaaacgg tcagcgtccg
      301 attgcgtttg tcagtgtggt tgatgacgaa atccgccgga ttattaaagg ggcggatgct
      361 tttcagatta atttctttga gacttttttg ggactgttgg agaaggaact caataccgaa
      421 gcaacggtat ccgggcaggg gcatcacagt atcggcaata cgaagcgtta cgatgcgcgt
      481 atggaggcag tcaatttttc tttgaaccat gatgacgggg tcagcgataa gaaccttcag
      541 gaggcggatg tgattctgat gggcgtgtcc cgttcgggca aaacgccgac ctgcctgtat
      601 ctggcgttgc aatacggtat ccgtgcggca aactatccgc tgattcccga cgatttggaa
      661 tcggccgatc tgccgcgtat ggtcaagccg tataaagaca aactgttcgg gttgactatc
      721 cagccggaac gtttgcaggc catccgtcag gaacgccgcc cgaattcggc ttatgcgcgc
      781 atcgacacct gccgcagcga agtggcggac gcgcagagta tgttcagacg gcatggcatt
      841 ccgtttgcga atacgacgga caagtcggtt gaggaactgg cggtacacat ccttcaggcg
      901 tgcaagctca aacgcaggtt ttgacgggct ttgattcggt ttgaaggtgg aaatgccgtc
      961 tgaaatcagg tttcagacgg cagttttatg tttgtggggc ggatattttt cagggctgta
     1021 ttttgtccag acattcgagc agatcgagcg gcgtgcggat gtggaaatcc gcctgccatg
     1081 agccggtatc gtcttcatcg gaaatatagc cccattcggc gaggacggtt ttcataccgg
     1141 cgttgcgtcc ggcttggatg tcgcgttccg cgtcgccgac gtagagggtg tgttgcgggt
     1201 cggcgtggat ttttccgcag gcgtgcagca tgggtttgat gctgggtttg ggttcgccgc
     1261 aggtgtcgcc gctgacgacg gtggcgggcg gaacggcgaa tccgagtttg ggaacgagtt
     1321 tgtcggtgaa gcgcatgggt ttgttggtga tgatgcccca tttgatgccg cgtctgccga
     1381 gttcggcgat gagttcgttc acgccgtcga agagagtggt gtcttgggcg tagcggctgt
     1441 cgtattcctc cagatattcg gtgcgccatg cggtatagtc ggggtgttcg ggcgtgatgt
     1501 tcgcgccgag tttgagcaat cctgccgcgc cgtggctggc ttgggtgcgg atttcgtcca
     1561 tgcttttttc gggcagtccg tggcgggcga gttgggtgtt gagtgcgccg ccgaggtcta
     1621 gggcggtgtc ggcgagcgtg ccgtcgaggt cgaacaatac ggcttgtatc atgtgttttc
     1681 cttttttata aagtgcggga cgaaaggttt cagacggcat gtttattttg tttcaaaccc
     1741 tgctcgaaat cttccaacat atccaattcg aagcggctga agcccgcttt ttcccgcgct
     1801 tcgatgttca catagccgcg gaagataaac atatcgtaac gggcaatcag actgcggaac
     1861 agggcgacag gttccaaacc gcgttcgcgg cagaggtgtt gataccaccg gttgccgatg
     1921 gcgacgtgtc ccacttcgtc gcggtaaatg atgtccaaca cgccgcaggt tgccgaatcg
     1981 ccgcgctgct ccaccttcgc gcgtatcccc ggcgtaacgt ccagtccgcg cgcctccaac
     2041 acgcgcggta ccaacgccat acgcaacagc gggtcgtagg cggttttgta cgccatatcc
     2101 cacaaatggt tgtgcgcctc gaaactgccg taatcgaagc cgaaagcgcg caatctgtcg
     2161 cgcaccaggc ggaagtggta cacctcctcc ttcgccactt tcacccagtc gcggacaaac
     2221 tgaaacggca gcgtgcggaa acggtatgcc gcgtccaaag ccagattgac ggcgttgaac
     2281 tcgatatgcg taatcgcgtg cagcatcgcc gcatagcctt ctgccgtgtt cattttgcgc
     2341 ggggtcagtt gcgagggcgc gaccaaaaca ggcgtgtccg gccgtcccgc gcgggggaag
     2401 tccgccggcg gcgcgtttgc ctccgccccg tccgcatttt gaacggcggc aaacgcctca
     2461 tccgtcagcc gtcctttttc atcggggtcg cccgaaagca gggcgcgttc cagcaaagca
     2521 taaatatcgg ggttcatctc aagtccgccg tgttcggaaa acgaatatta tagcgtttaa
     2581 aaaaaacaag atgaggcata taatctccgc gattcggcat tccgcgccca aaccgtcaaa
     2641 tatagtggat taacaaaaac cagtacggcg ttgccccgcc ttgccgtact atgttgtact
     2701 gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc actataacgc ggcatacaat
     2761 tgaaagggca gcgtggcgtg cctgcttttt ccgagcggtc aaaaaaatca gccctcggaa
     2821 aacgcggttt gcaaaatgca aaccgcccgt aacgccgccc gtatgattgt tttgccgcgc
     2881 cgatactttg cgccacaccc atcccgacaa ggaaaaataa tgatgaaacc gcacaacctg
     2941 ttccaattcc tcgccgtttg ctccctgacc gtcgccgtcg cttccgcaca ggcgggcgcg
     3001 gtggacgcgc tcaagcaatt caacaacgat gccgacggta tcagcggcag cttcacccaa
     3061 accgtccaaa gcaaaaagaa aacccaaacc gcgcacggca cgttcaaaat cctgcgcccg
     3121 ggcctcttca aatgggaata cactttgccc tacaaacaga ctattgtcgg cgacggtcaa
     3181 accgtttggc tctacgatgt tgatttggca caagtgacca agtcgtccca agaccaggcc
     3241 atcggcggca gccccgccgc catcctgtcg aacaaaaccg ccctcgaaag cagttacacg
     3301 ctgaaagagg acggttcgtc caacggcatc gattatgtgc gggcaacgcc caaacgcaac
     3361 aacgccggct accaatacat ccgcatcggc ttcaaaggcg gcaacctcgc cgccatgcag
     3421 cttaaagaca gcttcggcaa ccaaacctcc atcagtttcg gcggtttgaa taccaatccc
     3481 caactctcgc gcggcgcgtt caagtttacc ccgcccaaag gcgtggacgt gttgagcaac
     3541 tgatgccggc cgccccgatg ccgtctgaac gccgccgagg cttcagacgg cattttcacg
     3601 caggcggaac aatgtcccgc attatcggcc gatcgggcaa cggaacggca aatccgtgaa
     3661 aattaacggt tgcgccccgg ctgtttttgc cgtttaatgc aaaccttgct gcaccaaggg
     3721 ccaagaaagc cgaccggccg cccccacagc ttccgatgca ggcggcccgt ccgtccctgc
     3781 aatgtttttt atttttgaac gaaaggtcga aaaccatgaa aaaaacactg gtggcggcaa
     3841 tcctgagcct tgccttgact gcgtgcggcg gcggaagcga taccgccgcc caaaccccct
     3901 ccgccaagcc cgaagccgaa caatcgggca aactcaacat ctacaactgg tcggattatg
     3961 tcgatcccga aaccgtcgcc gcctttgaaa aagaaaccgg cattaagatg cgttccgact
     4021 attacgacag caacgaaaca ctggaggcaa aagtcctgac cggcaaatcc ggctacgacc
     4081 tgaccgcgcc gtccatcgcc aacgtcggcc ggcaaatcaa agcgggcgcg tatcagaaaa
     4141 tcgacaaggc gcaaatcccc cattacggca acatcgataa agatttgctg aaaatgatgg
     4201 aagccgtcga tccgggcaac gaatacgccg tcccctattt ctggggcatc aacaccttgg
     4261 caatcaacac gcggcaggtg caaaaggcat tgggtacgga caagctgccc gaaaacgagt
     4321 gggatttggt gttcaaaccc gaatacaccg ccaaactcaa atcctgcggc atcagctatt
     4381 tcgacagcgc aatcgaacag attcccttgg cgttgcacta tttgggcaaa gaccccaaca
     4441 gtgaaaatcc cgaagacatc aaagccgccg tcgatatgat gaaagccgtc cgaggcgacg
     4501 tgaaacgctt cagctcttcc ggctatatcg acgatatggc ggcgggcaac ctgtgtgccg
     4561 ccatcggtta cggcggcgat ttgaacattg ccaaaacccg tgccgaagaa gccgcaaacg
     4621 gcgtggaaat caaagtattg accccgaaaa ccggcgtagg cgtgtgggtg gattccttta
     4681 tgattccgcg cgacgcgcaa aacgttgcca acgcccaccg ctatatcgac tacacgctcc
     4741 ggcccgaggt ggctgcgaaa aacggcagct tcgttaccta cgcgcccgcc agccgtcccg
     4801 cgcgcgagct gatggatgaa aaatacacct ccgacgcatc gattttcccg accaaagaac
     4861 tgatggaaaa aagtttcatc gtatcaccca aatccgcaga atccgtcaaa ctgggcgtga
     4921 agctgtggca agggctcaaa gcgggcaaat aaccggaatc cctgccgtct gaaacctttc
     4981 gggcggcagg aaacggcgtg tccgcattaa gtcaggatta ggattattta gaaagatgag
     5041 atggatatga atttaagtat tgtcgttcct atttataatg tcgaaagtta tttggaagcg
     5101 tgtttaaatt ctatagaacc tatattaagt aatgaaaatg tcgaacttat tcttgtgaat
     5161 gacgggtcaa aa
//
LOCUS       DBJGGI010000099         5024 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.099, whole genome shotgun sequence.
ACCESSION   DBJGGI010000099 DBJGGI010000000
VERSION     DBJGGI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.099"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(<1..409)
                     /locus_tag="ACSIGJ_001978"
     CDS             complement(<1..409)
                     /locus_tag="ACSIGJ_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HHQ1330770.1"
                     /translation="MQPAKNLLFSSLLFSSAARAASEDGGRGPYVQADLAYAYEHITR
                     DYPDAAGLEKGKKISTVSDYFRNIRTHSIHPRVSVGYDFGGWRIAADYARYRKWNNNK
                     YSVNIKELERNENKNSGNHLNIKTQKTEHQENGT"
     gene            822..1742
                     /gene="ribF"
                     /locus_tag="ACSIGJ_001979"
     CDS             822..1742
                     /gene="ribF"
                     /locus_tag="ACSIGJ_001979"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HHQ1330771.1"
                     /translation="MKIRPGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVAVVFEPQPKEFFALRTGKTPPCRISPLRTKLGLLEKTGCVDAAWVLRFDRNF
                     SEISAQAFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLGGRVVHGRKLGRTLNAPTANIRLPGHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDGGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRRIEADMEAAKCW"
     gene            1884..4673
                     /gene="ileS"
                     /locus_tag="ACSIGJ_001980"
     CDS             1884..4673
                     /gene="ileS"
                     /locus_tag="ACSIGJ_001980"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HHQ1330772.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDNPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVAYPFKNTVA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     GALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMSVWEANPVILQWPEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGSDGKTLRDKAIKAVDDTEFFPPWG
                     RARLESMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVPQA
                     EMVEIDRYALVLARQLQERVAGDFYPRYAFHFAVKEMVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PAINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGDELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         1193 a         1365 c         1348 g         1118 t
ORIGIN      
        1 atgtgccgtt ttcctgatgt tccgtctttt gggtttttat gttaagatgg ttgccagaat
       61 ttttattctc gtttctttcc aactctttta tgttcacgga atatttattg ttgttccatt
      121 tcctgtaacg ggcataatct gccgctatcc tccagccgcc gaagtcgtag ccgaccgaca
      181 ccctggggtg gatggaatgc gtacggatgt ttctgaaata atcgcttacc gtgcttattt
      241 ttttgccttt ttctagaccg gctgcatcgg gataatcgcg ggtgatgtgc tcgtaggcgt
      301 aggctaaatc cgcctgcaca tacgggccgc ggccgccgtc ttcacttgcc gcccgcgctg
      361 cggaagagaa gagaagagaa gagaagagaa ggttttttgc gggctggatt cattttcggc
      421 tccttattcg gtttgaccgg ttaaaaaaaa agattttcac tgatgttgaa gggcggatta
      481 tatcgggttc cgggcggtgt ttcaatacat agcaccgcgc ctgctgcgcg ttttatgcgt
      541 ttggcgcgtt cggcggcggg aaatttgcct acttttcccg cgtcgggcgg gcggaacggg
      601 cggcacactg tctataaacc gcaataccgt ttacaatgac cgcctgtttc accacatacc
      661 cgaacgcaac aatgtttcaa cacacgggac ggcacatcaa gcaccgccct atgtgtcgtc
      721 ctgatttgga aggggttgca cccctcccga ataaagtctg atcctgccgc cccgaaggac
      781 ggatgtccga gtggcggggt ttcaaccgaa aaggaaatac aatgaaaatc aggccggggc
      841 ggcacaacgc gcccgacttt ccgcacgggg ccgccgtaac cataggcaat ttcgacggcg
      901 tgcacctcgg acacaaacac atcctccaaa aactccgcct cgaagccgat acgcgcggat
      961 tgcccgtcgt ggccgtcgtt ttcgaacccc aacccaaaga atttttcgca ctccgtaccg
     1021 gcaaaacccc gccgtgccgt atcagccccc tgcgtaccaa actcgggctg ctcgaaaaaa
     1081 ccggttgcgt cgatgccgcc tgggttttgc gtttcgatcg gaatttttcc gaaatatccg
     1141 cgcaagcatt tatcgaccgc ctgctgcgtc aaaccttgaa tacgcgctat ttgctcgtcg
     1201 gcgatgattt ccgtttcggt gcggggcggg aaggctgttt tgaacttttg gcacaacagc
     1261 ccgatatgca aaccgagcgc acgccttccg tcatcgtcga agacatccgc accagcagta
     1321 ccgccgtccg ccaagccctt tcagacggca accttgccta tgcgaagaaa cttttggggc
     1381 acgactacgt tttggggggc agggtggtgc acggcagaaa actcggacgc accttaaacg
     1441 ccccgaccgc caatatccgc ctgcccggcc accgttatgc actcggcggc gtgttcgtcg
     1501 tcgaggcgga cggcgcattc ggcacgcggc gcggcgtggc gagcttcggc ttcaatccca
     1561 ccgttgatgg cggctgttct caaaagcttg aagtccacct gttcgacttt caaggcgatt
     1621 tgtacggaca acggttgaac gtccgcttcc tgcacaaact gcgcgacgag gaaaagtttg
     1681 acggtatgga agaactgaaa aggcggattg aagccgatat ggaagccgca aagtgttggt
     1741 agaaaaacct tatacaaacc atccgattgg gctacaatca gccttttaac tgttcggacg
     1801 gcacaggggt ttcccgttgt gaaatactgt ttgaggcgca atgccgtctg aaaccgaaat
     1861 attgtaacaa tagagattaa aaaatgaccg attacagtaa aaccgtcaac ctgctcgaaa
     1921 gcccgtttcc gatgcgcggc aatcttgcca agcgcgagcc tgcgtggctg aaaagctggt
     1981 acgagcaaaa acgttaccaa aaactgcgcg aaatcgccaa aggccgtccg aaattcatcc
     2041 tgcacgacgg cccgccgtat gccaacggcg acatccatat cggtcatgcc gtcaataaaa
     2101 ttcttaaaga cattattatc cgcagcaaaa cccaagccgg ttttgacgcg ccttatgtgc
     2161 cgggttggga ctgccacggc ctgcccatcg aagtgatggt ggaaaaactg cacggcaaag
     2221 atatgcctaa agcccgtttc cgcgaattgt gccgcgaata tgccgccgaa cagattgccc
     2281 gtcagaaaaa agactttatc cgcttgggcg tgttgggcga ctgggacaat ccttacttga
     2341 ccatggattt caaaaccgaa gccgataccg tgcgtatgct cggcgaaatc tacaaatccg
     2401 gctatctcta ccgtggtgcg aaaccggttc agttttgctt ggattgcgga tcttcgctgg
     2461 cggaagcgga agtggaatac aaagacaaag tatcgcctgc gattgacgtt gcctatccgt
     2521 ttaaaaacac cgtcgcgctt gccgccgcat tcggcttggc aggtatcgaa ggcaaagcgt
     2581 ttgccgtcat ttggacgacc acgccttgga ctctgcctgc gagccaggcc gtgtctgccg
     2641 gcgcggacgt ggtgtatcaa ttaatcgata cgcccaaagg caaattggtg ctggcgaaag
     2701 atttggcgga aggcgctttg aaacgctacg gcttttcaga cggcatcgcc atccttgccg
     2761 aaaccaccgg cgacaagctg gaaaacctgc acatgaatca tccgttcctc gaacgcgata
     2821 ttcccatgct caacggcgaa cacgttacca ccgatgccgg taccggcttg gtgcatactg
     2881 cgcctgcgca cggtttggaa gactacgccg tctgcaataa atacggcatc gagctttaca
     2941 accctgtcaa cgccgaaggc aaatacataa gcgaaacgcc tcgtgtcgca ggcatgagcg
     3001 tttgggaggc gaatcccgtc atcctgcaat ggccggaaga aaccggcaac ctcttggcaa
     3061 gcagcaaaat cgaacacagc tacgcccact gctggcgcca caaaaccccg ctgatttacc
     3121 gagcgacagg tcagtggttt gtcggcatgg acaaagccgg cagcgacggt aaaaccctgc
     3181 gcgacaaagc catcaaagcc gtggacgaca ccgaattctt cccgccatgg ggtcgtgcgc
     3241 gtttggaatc catgattgaa ggccgtcctg actgggtggt ttcacgccaa cgctattggg
     3301 gcacgccgat gactttcttt gttcacaaag aaaccggtga attgcatccg aactctgccg
     3361 aacttttgga aaaagtcgcg caacgcatcg aagaaaaagg catcgaggct tggttctccc
     3421 tcgataaaag cgaattatta agcgcggaag attgcgaaca ttacgacaaa ctccccgata
     3481 ccatggacgt atggttcgac tcaggctcga cgcattattc cgttgtaaaa caacgcgaag
     3541 aattggaatg gccggctgac ttgtacctcg aaggcagcga ccaacaccgc ggctggttcc
     3601 aatcttctat gctgaccggt tgcgcctcat ccatgggacg cgcaccgtat aaacagctgc
     3661 tgacccacgg tttcgttgtg gacggcgaag gcaaaaaaat gtcgaaatcc atcggcaacg
     3721 tcgtcgcgcc gcaggaagtg tataacgagt tcggcgcgga catcctgcgt ctgtgggcgg
     3781 catccaccga ttacagcggc gaattggcga tttccaaaga aatcctcaaa cgcgtaaccg
     3841 aaagctaccg ccgtatccgc aataccttga gcttcctgtt tgccaatttg agcgacttta
     3901 atccgattga agatgctgtt ccacaagcag aaatggtgga aatcgaccgc tacgccctgg
     3961 tgttggcgcg tcaacttcaa gaacgcgttg ccggcgattt ttatccgcgt tacgccttcc
     4021 attttgccgt aaaagaaatg gtttctttct gctcggaaga cttgggcgcg ttctacctcg
     4081 acatcctgaa agaccgcctc tacaccacca aagccgacag ccgcgcccgc cgcagcgcgc
     4141 aaaccgccct gtaccacatc acgcgcagcc tggttctctt gattgcaccg attttgtgct
     4201 tcaccggcga agaagcgtgg gacatcatcg gcggcggcga agaagacagc gtcctcttcc
     4261 atacttggca cgagttcccg gccatcaacg aaaaagccga agccgaactg gtgaaaaaat
     4321 ggacggcaat ccgcgaagcg cgcgaagcag taaccgccgc catcgagcct ttgcgcgccg
     4381 acaaaaccgt cggttcgtcc ttgcaggcgg aagccgaaat caccgcgccg gaagaaatgg
     4441 ccggctatct gaatgctttg ggcgaagaat tgcgctttgc cctgctggtg tctaaagcag
     4501 aagtgaaagt cggtgatgaa cttgccgttg ccgccaaagc cagcgacggc gaaaaatgcg
     4561 aacgctgctg gcattacacc cgcgatgtgg gcgcggttgc aggttatgaa accgtctgca
     4621 aacgctgtgc ggagaatgtc ggcggagaag gcgaaacgcg ccattacgcc tgataaagtt
     4681 tgagcaaatg ccgtctgaaa ccgccaaaca gtatttcaga cggcattttt tgtgccgcga
     4741 tttgtcttca taatggcgga ggggtttcga gattgcggtg ttgtcgggaa tgacggttca
     4801 gttgctacgg ttactgtcag gtttcggtta tgttggaatt tcgggaaact tatgaattgt
     4861 cattcccgcg aaagtgggaa tccaggactc aaaatctcaa gaaaccgttt tgcccgataa
     4921 gttcctgcac tgacagatct agattcccgc ctgcgcggga atgacgggtt tcgagattgc
     4981 ggtgttgtcg ggaatgacgg gttttaagat tgcggtgttg tcgg
//
LOCUS       DBJGGI010000100         4934 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.100, whole genome shotgun sequence.
ACCESSION   DBJGGI010000100 DBJGGI010000000
VERSION     DBJGGI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.100"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..550
                     /locus_tag="ACSIGJ_001981"
     CDS             <1..550
                     /locus_tag="ACSIGJ_001981"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HHQ1330773.1"
                     /translation="RFFPQPRETAALRAKYGFADHETVFLFPSTGHTRKGLELLADFF
                     EQTELPVKLAVAGSPLPRPMKNVVGLGFCTDMPELYRAADFTIMASLYEPFGLVGVES
                     ALCGTRVVLSENMACTEVMNEEAGFFFSRQNPETLAQAVARAVSLKKQGGHRLSDPMR
                     ALNYNPSLSHHIDRLTDMLASV"
     gene            complement(617..2269)
                     /locus_tag="ACSIGJ_001982"
     CDS             complement(617..2269)
                     /locus_tag="ACSIGJ_001982"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HHQ1330774.1"
                     /translation="MVAYAFLFLFVTAVVLLIVRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIHYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADSLGMPWRWAGAVLFAAAFVSVRHFSKHPGAVKTWLDSL
                     PDDGRDVFLNLPMSCRAVFFQVPVFEGDGEAFARQMPSETRPYGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGFVRNLREHGYFCVALSPFTKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDKVFDLDAPDLNAKTVSALNDYIGRIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQDFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNRELVGNYRNYLYDVLKIAR"
     gene            2412..2975
                     /gene="gmhB"
                     /locus_tag="ACSIGJ_001983"
     CDS             2412..2975
                     /gene="gmhB"
                     /locus_tag="ACSIGJ_001983"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HHQ1330775.1"
                     /translation="MKLIILDRDGVINRDRDDFVKSVDEWIPVEGSMDAVAFLTQAGC
                     TVAVATNQSGIGRKYFTVQDLTEMHAKMHRLIRQAGGEIDGIWFCPHTGADGCNCRKP
                     KPGMIEDILERFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PGHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            3005..3748
                     /locus_tag="ACSIGJ_001984"
     CDS             3005..3748
                     /locus_tag="ACSIGJ_001984"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HHQ1330776.1"
                     /translation="MLIIRNLIYWLILCSSLIFLFPFMLLASPFRDGAHKMARVWVGI
                     LNWSLKHIVGLKYRIIGAEHIPDRPSVICAKHQSGWETLALQEIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVIICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAEMPSETA"
     gene            3745..4434
                     /locus_tag="ACSIGJ_001985"
     CDS             3745..4434
                     /locus_tag="ACSIGJ_001985"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HHQ1330777.1"
                     /translation="MTSLIHTLSDGIELTVEINRRAKKNLIIRPIGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRRTLAKTPPHNTANRLPEHIWFHGRQLALTAHQDTQILLMPS
                     EILVPEGAPEKQLALLRDFLERQAHSYLIPRLEHHARATQLFPASSSLTSAKTFWGVC
                     RKTTGIRLNRRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPEAKQRI
                     KIHGRELFALG"
     gene            4516..4647
                     /locus_tag="ACSIGJ_001986"
     CDS             4516..4647
                     /locus_tag="ACSIGJ_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HHQ1330778.1"
                     /translation="MPPVRGRMAASAPSAPFHFTPPFRNPPDPTCGMKRLSRREVFY"
BASE COUNT         1198 a         1603 c         1193 g          940 t
ORIGIN      
        1 acgcttcttt ccacaaccta gagaaactgc cgccctgcgc gccaaatacg gttttgccga
       61 ccatgaaacc gttttcctat tcccatcgac cggccacacg cgcaaaggcc tggaactgct
      121 cgccgacttt ttcgaacaaa ccgaactgcc cgtcaagctc gccgttgccg gttcaccgct
      181 tccccgcccg atgaaaaacg tcgtcggact gggcttctgc accgatatgc ccgaactcta
      241 ccgcgccgcc gactttacca ttatggcatc cctgtacgaa cccttcgggc tggtcggtgt
      301 cgaatccgcc ctatgcggca cacgcgtcgt cctctccgaa aacatggcat gtacagaggt
      361 catgaacgaa gaagccggat tctttttctc acgacaaaac ccggaaaccc tcgcgcaagc
      421 cgttgcccgg gccgtcagcc ttaaaaaaca gggcggacac cgcctgtccg acccgatgcg
      481 ggcgctgaac tacaacccgt ccctgtcgca ccacatcgac cgactgaccg acatgctggc
      541 atccgtataa caaaatgccg tctgaacggg ttttcagacg gcattttcgc aaccgttgca
      601 gccgtgcggc aaaaagctaa cgggcaattt tcaaaacgtc gtacagatag ttgcggtaat
      661 ttccgaccaa ctcccggttc gggcagtctt ccaaccctcc gccgcacaaa ccgcgcatcg
      721 ccatattcgc acgcatgaag ccgtctttgg cttccaaacc agccgcctcc atcagtacgc
      781 cgcctgcaaa ggcaaggtcg aggaaatcct gccgctgtac gaatccgccg gcaatattgc
      841 tcctgacggc aaactgcgtt acataatccg gctgagcgta atcccatttc ttcctgacgg
      901 acacgccctc aaacggtacc tgatgatcgc cgaaataacc gaaaacaaag ggtttgccgc
      961 gttcgtgcag ataacggtcg aaactttcca ccgctttgtc caaatcggca atgcgcccga
     1021 tgtagtcgtt aagcgcggat acggttttcg cattcaaatc gggcgcatcc aaatcaaaca
     1081 ctttgtcggt atccgtccga tacggcccgt gctccttcat ggtcagcaca tatacgaaca
     1141 tcggctgccg cacgttttcc aaatcgggat ggcgtttttc gagaatcatc cgcgcatact
     1201 gcatcatttc ctcactggaa atgtgccaca ggtttttgcc catcggcgcg ggatagccca
     1261 aatcttgcgg ctggaacatc agattaaagc cgaaatggtc atatgctgcc ttggcattgt
     1321 agttgccctt ggtaaacggc gagagcgcca cgcaaaaata accgtgttcg cgcaggttgc
     1381 ggacaaaacc ggtctgcaaa tgcggtacga ccgaataaaa cacgccgctt gccaacgcgc
     1441 cgaaatctgt ggacggaaca cccgccaaaa acgcaaattc ggacttccac gttgcgccgc
     1501 cgaaagtatg cacgcgcaaa ggtgaggaaa atacagtatc ttcctgccgt ccgaacattt
     1561 tcaaatcggg aattttggcg gcggcaaaat cgaaacagtg cggatcgagc gtcgattcca
     1621 tcagggtaac gacaatatcg ggcttttcat ccgacatgcc gtacggccgg gtttcagacg
     1681 gcatctgcct ggcaaacgct tccccgtcgc cctcgaacac gggtacttgg aaaaacaccg
     1741 cccgacagga catcggcagg ttcaaaaaca cgtcgcgccc gtcgtccggc agcgaatcca
     1801 gccacgtctt taccgcgccg gggtgcttgg aaaaatgccg caccgacacg aacgccgccg
     1861 caaacagaac cgcgcccgcc caacgccacg gcatacccaa agaatccgca ccgctccagc
     1921 cgaaaaccgc atatgccgcc aaagccagca gccccgccat accgataacc gcttccttat
     1981 aatgaaacag cgtttcccaa ttccgccagt cggcaatcag caaaaagtcg gaaatcaaca
     2041 gcggctgttt gtaataatgg attttcagcc tgtggaacag tatcagcacc acaaaccaga
     2101 ccgaagcgaa attcaaggcg cgctgccact gcgccgtcaa catcagcata ccgcccgcca
     2161 aaaagacaaa cagcgccgac gcgaaaaaat acgtccagcg gtagtgcgac ctgacaatca
     2221 gcagcaccac cgccgttaca aacaaaaata ggaaagcata ggcaaccatc ccgccccact
     2281 cctcaaaacg gcatacctgt gccgttgcga attattatcg aaagcgtgca ttataatgca
     2341 gcacaggcgc atttccaccg atatttcagt ataatgccat ccccgaaccc gcccaaccca
     2401 aaggaaccgt gatgaaactc atcattctcg accgcgacgg cgtcatcaac cgggaccgcg
     2461 acgacttcgt caaatccgtt gacgagtgga tacctgtcga aggcagcatg gatgcggtgg
     2521 cattcctgac gcaggcaggc tgcaccgttg ccgtcgccac caaccaatcc ggcatcggcc
     2581 gcaaatattt taccgttcaa gacctcaccg aaatgcatgc caaaatgcac cgcctcatcc
     2641 gtcaggcggg cggggaaatc gacggcatct ggttctgccc gcataccggc gccgacggct
     2701 gcaactgccg caagcccaaa ccgggcatga ttgaagacat cctcgaacgc ttcaacgccc
     2761 aagcttcgga aacttggctg gtcggcgaca gcctgcgcga tttgcaggca atcgatgccg
     2821 tcggcggaaa acccgcgctg gttctgaccg gaaaaggcaa aaaaacgctc tcccaacacg
     2881 gacacgaatt acccggacac acacaggttt tcgataccct gctcgatttc tcacaataca
     2941 tcatgcagga aaacgccgca ccgcaagccg actgaacata ccgcatttcc gacaaggcaa
     3001 aaccatgctc atcatccgca acctgattta ctggctgata ctctgttcca gcctgatttt
     3061 cctctttccc tttatgctgc tcgcctcgcc tttccgggac ggggcgcaca agatggcgcg
     3121 ggtctgggtc ggcatcctca actggtcgct caaacacatc gtcgggctca aataccgcat
     3181 catcggcgcg gaacacattc cggaccgccc ctccgtcatc tgcgccaaac accaaagcgg
     3241 ctgggaaacg ctcgcgctcc aagagatttt tccgccgcag gtttacgttg ccaagcgcga
     3301 gttgttcaaa atcccctttt tcggctgggg cttgaaactg gtcaaaacca taggcataga
     3361 ccgcaacaac cgccgcgaag ccaacgaaca gctcataaaa cagggtttgg cgcgcaaaaa
     3421 cgaaggttat tggattacca ttttccccga aggcacgcgc cttgcgcccg gaaaacgcgg
     3481 caaatacaaa ctcggcggcg cgcgcatggc gaaaatgttt gagatggaca tcgtccccgt
     3541 cgccctcaac agcggcgaat tttggccgaa aaattccttt ctgaaatatc cgggggaaat
     3601 caccgtcatc atctgtccga ccatcccgca cgcaagcggc agcgaagccg aattgatgga
     3661 aaaatgcgaa catctcatcg aaacgcagca gccgctcatt tccggcgcag gcccgtttgc
     3721 cgccgaaatg ccgtctgaaa ccgcatgacc tccctgattc acaccctttc agacggcata
     3781 gaactgaccg tcgaaatcaa ccgccgtgcc aagaaaaacc tgattatccg ccctatcggc
     3841 acacataccg tccgcatcag cgtcccaccc tgcttctccg tctccgctct aaaccgctgg
     3901 ctgtatgaaa acgaagccgt cctgcggcgg acactggcga aaacaccgcc gcacaatacg
     3961 gcaaaccgac tgcccgaaca tatctggttc cacggcaggc agcttgccct taccgcccat
     4021 caagacacgc aaatcctgct gatgccgtct gaaatcctcg tccccgaagg cgcgcccgaa
     4081 aaacagcttg cgctgctgcg ggactttttg gaacggcagg cgcacagtta cctgattccc
     4141 cgtctcgaac accacgcccg cgccacgcaa ctgttccccg cctcctcctc gctgacctcc
     4201 gccaaaacct tctggggcgt gtgccgcaaa accacaggca tacgcctcaa ccggcggctg
     4261 gtcggcgcac ccgaatacgt tgccgactat gtctgcatac acgaactctg ccacctcgcc
     4321 catcccgacc acagccccgc cttttgggaa ctgacccgcc gcttcgcccc ctacacgccc
     4381 gaagcaaaac agcggatcaa aatccacggc agggagcttt tcgccttggg ctgacgcagc
     4441 cccgggccga cgctttcaga cggcatccgt gccggaacag gcacgcgccc gcccgattca
     4501 aaccgcgatg acgctttgcc gccggttcgg ggcaggatgg cggcatccgc gccgtccgca
     4561 ccgtttcatt tcacaccgcc cttccgaaac ccgcccgatc cgacgtgcgg tatgaaacgc
     4621 ttgagccggc gcgaagtctt ctactgattt gcccgcgaaa ataccgtccg aaaggttttc
     4681 ggacggcatt tttttgcgtt ttcctggatg gcggcggatt cgtaaaaggc gggcagggtg
     4741 gattgcagga tgggttgagg cctgccaaat ccgccaactc taccgaatac gccgccgtca
     4801 ttcccgcgca ggcgggaatt tggttttgtc ggtgcggaaa cttatcggat aaaacggttt
     4861 cttagattcc acgtcctaga ttcccgcctg cgcgggaatg acggttcggg tatttctgac
     4921 gattcatcga gccg
//
LOCUS       DBJGGI010000101         4516 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.101, whole genome shotgun sequence.
ACCESSION   DBJGGI010000101 DBJGGI010000000
VERSION     DBJGGI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.101"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(38..763)
                     /gene="aat"
                     /locus_tag="ACSIGJ_001987"
     CDS             complement(38..763)
                     /gene="aat"
                     /locus_tag="ACSIGJ_001987"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HHQ1330779.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSRDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDENGKTRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(833..1267)
                     /gene="fur"
                     /locus_tag="ACSIGJ_001988"
     CDS             complement(833..1267)
                     /gene="fur"
                     /locus_tag="ACSIGJ_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HHQ1330780.1"
                     /translation="MEKFSNIAQLKDSGLKVTGPRLKILDLFEKHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            1494..1871
                     /locus_tag="ACSIGJ_001989"
     CDS             1494..1871
                     /locus_tag="ACSIGJ_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990063 - Bam protein complex [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HHQ1330781.1"
                     /translation="MNKTLILALSALFSLTACSVERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDALQNAAEALRAKQNADKQ"
     gene            1881..2690
                     /gene="dapB"
                     /locus_tag="ACSIGJ_001990"
     CDS             1881..2690
                     /gene="dapB"
                     /locus_tag="ACSIGJ_001990"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HHQ1330782.1"
                     /translation="MIPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIRAAAEKTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2804..3082)
                     /locus_tag="ACSIGJ_001991"
                     /pseudo
     CDS             complement(2804..3082)
                     /locus_tag="ACSIGJ_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3164..3322)
                     /locus_tag="ACSIGJ_001992"
     CDS             complement(3164..3322)
                     /locus_tag="ACSIGJ_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330783.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNETELARMGLRL
                     KYNGSLTD"
     gene            complement(3330..4502)
                     /gene="lpxB"
                     /locus_tag="ACSIGJ_001993"
     CDS             complement(3330..4502)
                     /gene="lpxB"
                     /locus_tag="ACSIGJ_001993"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HHQ1330784.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARL
                     TGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRELVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRAGIPTLHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARKTLGADVGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAARFLLPAATEATKRRLAEVLQRPEFAGLALTVTDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQSEAKPEKLAAALADWYEHPDKVAALQQDFGALHLLLKKDTADLAARAVLEEAGC"
BASE COUNT         1125 a         1457 c          996 g          938 t
ORIGIN      
        1 ccactatatt tgaacctgac cgaagcataa gccgccatca aagccccttg cacgccactt
       61 cgcgccgccc gatttcctct ttcaacggca cggcgttcaa catccgcaga cgttcggcaa
      121 aatccgcaaa cggcagcagc tccgaaccga aacggcgcat atgttccgta tcctgctggc
      181 agtctatcag ttccacgccc aaatccgcca aaaacggcac ggcgcaggca aacgcgattt
      241 tcgacgcatc cggttgcaac gcaaacatcg attcgccata aaacaccctg ccgatctgaa
      301 cgccgtaaaa gccgcccgcc aacctcgttt taccgttttc atcgggataa tggcactcga
      361 aagaatgcgc gtgtcccatt tcgtgcagct tcaaatatgc cgtctgaaac tcgggcgcaa
      421 tccaagtccc atcctgattc gggcgcgccg ctgccgcaca atgcgcgaca acttccgcaa
      481 aacagccgtt gaccgcaacc cgatagctgc cgttacgcag cgttttcgcc agcgagcgcg
      541 gaacgtgcag acgttcggga acaattaccg cacgcggccc gaccgcatac caaaaaaacc
      601 aaccgtcccg cgaaaaccaa ggaaacacgc cgttccgata cgcctcgagc agccgccccg
      661 cgtccaaatc cctgctcacg ccgaccagcc cgtcgcaccg ggccaaagca taggcaggat
      721 cgggaaaggc ataattgtca ggggcaagca gcggaatacg catggcagtt ctccaaaaac
      781 acagacggca tccaaaatca tgaatgccgt ctgaacaaca aaccgtccgg atttaacgtt
      841 tgcccttggc ctgacagtcg ctgcacacgc cgtacatata aagcgcgtga tcgacgatgc
      901 ggtagccgtt ttcctcggcg attttgtctt gcagggcttc gatttcggga ttgtggaatt
      961 ccgttacctc gccgcacttc acgcagacga tatggtcgtg gtggtcgccc ttgtccaact
     1021 cataaaccgc cttgcccgtt tcaaaatggt ggcgttgcag aatgcccgcc tgctcgaact
     1081 gcgtcagcac gcggtaaatc gtcgccacac cgatttccac gccctcttcc agcagaatgc
     1141 ggtacacatc ttccgcactc aaatgctctt ccgcgtgctt ctcgaacaaa tccaaaatct
     1201 tcaaacgcgg gccggtaacc ttcagaccgc tgtctttcag ttgcgcaatg ttgctgaatt
     1261 tttccataat attcaatatc cctgtaaaat aatagacgct ataatacgca atttcagcct
     1321 gcttgcccac tatcacacca tagcagttcg caatagcaaa accgcaacgg cggacgggca
     1381 gtcggacaca gacagggtaa aataccgctt aagcgtatga ttatcgttcg cttttataaa
     1441 atattcaagc agtgctacac tagacatccc gatttgcaca gaaaggttct cccgtgaaca
     1501 aaaccctcat cctcgccctt tccgccctgt tcagcctgac cgcgtgcagc gtcgaacgcg
     1561 tctcgctgtt tccctcctac aaactcaaaa tcatccaagg caacgaactc gaaccgcgcg
     1621 ccgttgccgc cctgcgcccc ggcatgacca aagaccaagt cctgctcctg ctcggcagcc
     1681 ccatactgcg cgacgctttc cataccgacc gctgggacta taccttcaac acctcccgca
     1741 acggcatcat caaagaacgc agcaacctga ccgtctattt tgaaaacggc gtactcgtcc
     1801 gcaccgaagg cgacgccctc caaaatgccg ccgaagccct ccgcgcgaaa caaaacgcag
     1861 acaaacaata aggaacacac atgataccgc tcaaaatcgc catcgccggc gcaaacggcc
     1921 gcatgggacg cgtcctcgtc gaagccgtca acaaccatcc cgacaccgtc ctttccggcg
     1981 cgctcgaaca ctcaggctca gaagccctcg ggctggacgc gggctacgcc gtcggactca
     2041 aaaccggcat cgccatttca gacgacgttg acgccgttct cgcccaaagc gacgtactca
     2101 tcgacttcac ccgccccgag ccgaccctca agcacctgca aaaatgcgtt gaaaaacaag
     2161 tcaacatcat catcggcaca acaggcttcg acgatgcggg caaagccgcc atccgcgccg
     2221 ccgccgaaaa aacaggcatc gttttcgccg ccaacttcag cgtcggcgtc aacctcacct
     2281 tccacatcct cgacaccgtc gcccgcgtcc tcaacgaagg ctacgacatc gaaatcatcg
     2341 aaggccacca ccgccacaaa gtcgatgccc ccagcggcac agcattacga atgggcgaag
     2401 tcatcgccgg cgcgctcggc cgcgacctca aacaatgcgc cgtttacggc cgcgaaggcc
     2461 acactggtcc gcgcgatcct tccaccatcg gctttgccac cgtccgcgca ggcgacatag
     2521 tcggcgacca caccgccctc ttcgccaccg acggcgaacg cgtggaaatc acccacaaag
     2581 ccggcagccg catgaccttt gccgccggtg ccgtccgcgc cgcagtttgg gtaaacggca
     2641 aaacgggttt gtacgatatg caggacgtac tcgggctgaa caaccgttaa cccccataca
     2701 aaatgccgtc tgaaaagata ttgttcagac ggcattttgc cgacaggctt cgtatcggca
     2761 tatcaataaa gttgcttgtg cagcatgaca agctacggct cgtttaattg aaactctcct
     2821 gatctaaaaa ttccaactct atttcccggc aaactatatc tatgataaaa ctggtcggaa
     2881 gattatacct acgccttatt aatgccagtg cacctccata atccaagatt tcatcatcct
     2941 gctttaattc tccaagctcc aaggtattat cgatatcttt atcttggttc tccgtagcgt
     3001 atccatagat aaatttcaaa tagctgaact gcttgaattt aatttttacc cagtctgtgt
     3061 aatttggtgt aggacacaat tgcctaaaat agaaatttac gaagaacaaa tgaaacaagg
     3121 tacgtaagcg tattagtata aactataatc atatataata aaattaatca gttaacgaac
     3181 cattatattt taaccttaat cccattcggg ccagttctgt ttcatttgga attcctctta
     3241 aatacttatt gtttgatgtg ccattaatca caatttctcc cccttttttt gtaatacgtg
     3301 ctgcttcggg aaaataatcc attaaccgct caacatcccg cctcttccaa aaccgcacgc
     3361 gcggccaaat ccgccgtatc ttttttcaac agcaggtgca acgccccgaa atcctgttgc
     3421 agcgcggcaa ccttatcggg gtgttcgtac cagtccgcca atgccgccgc cagtttttcc
     3481 ggttttgctt cagattgcaa taattccggc acggcctcct tacccaacag gatattcggc
     3541 aggccgacat gcggcacttt gattttgcgt ttcacataag cataggtcag cggcgaaatc
     3601 ttgtagctga tgaccatcgg acgcttgcac aacgccacct ccaaagtcgc cgtaccgctc
     3661 gttaccagca ccgcatccgc cgccctgcac accgtttcag actgtctgtc ggttaccgtc
     3721 agcgccaatc cggcaaactc cggccgctgc aaaacttccg ccaaacgccg cttcgtcgcc
     3781 tccgttgcgg caggcagcag gaagcgggcg gcgggatagc gttccaacaa caataatgcc
     3841 gtctgaaaaa acaccggcgc catatagtcg atttcgctga cgcggctgcc gggcagcagg
     3901 gcgaatacgg ggatgccgac atccgcgccc aaagttttcc gcgccgtttc acggtcgtct
     3961 tccaagggca taagctgtgc catcggatga ccgacaaact ccgcacgtcc gcccgcatcg
     4021 agataaagct gcggctccat cgggaacagg cacagcacgc ggttgacctg atgcacgatt
     4081 ttgcccacgc gttcgcgccg ccacgcccaa accgacggac tgacataatg cagggtcgga
     4141 atgcccgccc gtttcagctt ttccgccaca cccagattaa aatcgggcgc atcgataccg
     4201 acaaagacat caggtttcaa cgacagcaaa tcccgtacca gctccctgcg tatccgtaaa
     4261 atttccggca gccgcctgac cacttcgaca aagccgcgca ccgccagccg ctcctgatca
     4321 taaaggctct cgaaaccttc cgccttcatc agttccccgc cgatgccggt taaccgtgcc
     4381 tgcgggcaac gctttcggat ggcgcgtatc aggtgcgccc ccaacaggtc gcccgacgct
     4441 tcgccgacac tgacggcaat caaagggctt tttttatcaa ccatattcgt ctgtccccac
     4501 atatagtgga ttaaca
//
LOCUS       DBJGGI010000102         4491 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.102, whole genome shotgun sequence.
ACCESSION   DBJGGI010000102 DBJGGI010000000
VERSION     DBJGGI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.102"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..81
                     /locus_tag="ACSIGJ_001994"
     CDS             <1..81
                     /locus_tag="ACSIGJ_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HHQ1330785.1"
                     /translation="LRECEFRLNFGTPSRQLKILRDRCGI"
     gene            complement(49..1317)
                     /locus_tag="ACSIGJ_001995"
     CDS             complement(49..1317)
                     /locus_tag="ACSIGJ_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330786.1"
                     /translation="MKQKKTVQCILLGFAAASMHAQGAAAANSGTIEKTDKYTLVLAK
                     QGQENNYTLNGGTEVKPLNSLIIAANGGTNNITIKGKLADGPADAPPTIDNNSIERNI
                     NKNGYTYAWQNWSGAVMLVDQSYEGENKVTFENVTIAAHNAPAGILSDDRHKSSSLAP
                     AMLAFKGRNTINMDADSNANSSNEGILLLNNGEKMGEYRLVSEEGSTLNINIKSGKDK
                     GQGITANHYGNSDINFNKASPNITTMEFKGDVNIKIDRNGQEEAESNGFGFYSSRKLG
                     NKKQIPEGSKMEAIFRGNVDIVATPVYDEQGRPKSIGSAFAIDGKYSKVEVVGGEGKV
                     VKIKGDIFAYNGGSVSVNLANKDSYFEGEAHIGKRSFAKGKDMFALTVDADGYELTPD
                     TKSIEKKKKELNVRGCTRLSLNSTPIPQDF"
     gene            1514..1589
                     /locus_tag="ACSIGJ_001996"
     tRNA            1514..1589
                     /locus_tag="ACSIGJ_001996"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1548..1550,aa:Glu,seq:ttc)
     gene            complement(1805..1957)
                     /locus_tag="ACSIGJ_001997"
     CDS             complement(1805..1957)
                     /locus_tag="ACSIGJ_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330787.1"
                     /translation="MPSENRSAGFVVHYYVVLQDKAQGKAAGCIVATDNEKRGRCRLN
                     RVLRRV"
     gene            2017..2823
                     /gene="thiD"
                     /locus_tag="ACSIGJ_001998"
     CDS             2017..2823
                     /gene="thiD"
                     /locus_tag="ACSIGJ_001998"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HHQ1330788.1"
                     /translation="MKGSFVQTLAIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCSFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDILTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVIIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
     gene            complement(2881..3750)
                     /gene="tehB"
                     /locus_tag="ACSIGJ_001999"
     CDS             complement(2881..3750)
                     /gene="tehB"
                     /locus_tag="ACSIGJ_001999"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HHQ1330789.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKVENLPEVLLSGYSSEEGEWVCL
                     NVLQGDVEVRAPDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAAQDTVPAGRALDMGCGQGRNALFLGLKGFDVTAADCNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVMMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPG"
     gene            3863..4300
                     /gene="rnhA"
                     /locus_tag="ACSIGJ_002000"
     CDS             3863..4300
                     /gene="rnhA"
                     /locus_tag="ACSIGJ_002000"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HHQ1330790.1"
                     /translation="MDTPVYLYTDGACKGNPGAGGWGVLMRYGSREKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWQELDALVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
BASE COUNT         1037 a         1259 c         1065 g         1130 t
ORIGIN      
        1 ttgagggaat gcgaatttcg acttaacttc ggcacaccgt cccggcagct aaaaatcctg
       61 cgggatcggt gtggaattta gggataatct agtacagccc ctaacattca gctctttctt
      121 tttcttttca atcgactttg tatcgggggt taattcatat ccgtccgcat ccacggttaa
      181 ggcaaacata tctttccctt tggcaaagct ccttttcccg atatgggctt ccccttcaaa
      241 ataagagtct ttgttggcaa ggttcacgct cacgctgccg ccgttgtagg cgaaaatatc
      301 gcctttgatt ttgacaacct tgccttcccc gccgacaacc tcgactttgc tgtatttccc
      361 gtcaatcgca aacgcgctgc ctatgctttt gggcctgcct tgctcgtcat aaaccggcgt
      421 tgcaacgata tcgacgttcc cacggaaaat cgcttccatc ttagaacctt ccggaatctg
      481 cttcttattg cctaatttgc ggcttgaata gaagccaaaa ccgttgcttt ccgcctcttc
      541 ctgtccgttc ctatcgattt taatgtttac atcgcccttg aactccattg tggtgatgtt
      601 tggagaagcc ttgttgaagt tgatgtctga gttaccatag tggttggcgg taatgccctg
      661 tcctttatct tttcctgatt tgatgttgat attcagcgtc gagccttctt cggaaacaag
      721 acgatattct cccatttttt caccattatt aagcaatagg ataccttcgt tactagagtt
      781 ggcatttgag tccgcgtcca tattgatggt attgcgccct ttaaaggcaa gcatcgccgg
      841 cgccaagcta ctggatttat gcctatcatc agacaaaatg ccggcaggcg cgttatgggc
      901 ggctatggtt acgttttcaa aagtaacctt attttctccc tcatagcttt gatccaccaa
      961 catcaccgcg ccgctccaat tctgccatgc gtacgtgtaa ccgtttttat ttatatttct
     1021 ttcaatcgag ttattgtcaa tcgtcggcgg ggcatcggca ggaccgtctg ccaatttgcc
     1081 ttttatcgta atgttgttcg taccgccgtt ggcggcaatg atgagggaat ttaagggttt
     1141 gacttcggtt ccgccattaa gggtatagtt gttctcttgc ccttgttttg cgaggacgag
     1201 ggtgtatttg tctgtcttct ctatcgttcc actgtttgca gcggccgccc cttgcgcgtg
     1261 catagaagcg gccgcgaagc caagtaagat gcactgaact gtcttctttt gtttcatcgg
     1321 tttttccatt ttgaaggtat gtgattttta tcattatatg cttaaatcat tgacacgccc
     1381 ccaaaaaaaa tgaattgctt gatattccaa tgttttttat cttgtgatac ggtcagacaa
     1441 acgccccctg acattcgttt agacggcatc gtattgctaa atttctataa gtatgtataa
     1501 tgtccgtttc cacgcgccca tcgtctagag gcctaggaca ctgccctttc acggcggcaa
     1561 ccggggttcg aatccccgtg ggcgtgccaa ttcaaaaacc tgcttgtttc aagcaggttt
     1621 tttattatga atcgttattc ccgcaatttt tcgtcattgc cgtgaaaacg ggaatctaga
     1681 acctcaaact ttcagataat ctttgaatat tgctgttgtt ctaaggtccg gattcccgcc
     1741 tgcgcgggaa tgacggaatt ttaggtttct gtttttgcgg gaatgacgaa tgaagcgtgc
     1801 cggtttatac tcgccgcaac acgcggttca gacggcatcg ccctcttttt tcattatcag
     1861 ttgctacgat gcaccccgct gccttgccct gtgccttgtc ctgcaatacg acataataat
     1921 gcaccacaaa ccccgcgctg cggttttcag acggcattgc ctgctttttt acaggcagta
     1981 gcccttttta tcggacgcaa tattaaggag gaacaaatga aaggctcttt tgtgcagacg
     2041 cttgccatcg ccggttcgga ttcgggcggc ggtgcgggca ttcaggcgga tttgaaaacg
     2101 tttcagatgc gcggcgtgtt cggaacgtgt gtcatcaccg ccgttaccgc gcaaaatacc
     2161 ttgggcgtgt cggcggttca tctcgtcccg accgaaacca tcaccgcaca aatccaagca
     2221 atcagggaag actttgacat ccgcgcctac aaaatcggta tgctcggcac ggcggaaatc
     2281 atcgaatgcg ttgccgacaa gctgaaacac tgcagctttg gcaggcgcgt actcgaccct
     2341 gtgatgattg ccaaaggcgg tgcgccgctg ttgcaggatt ccgccgttgc ggcactgacg
     2401 cgcctgctgc tgccggacac ggacatcctg acccccaacc tgcccgaggc ggaagccttg
     2461 acgggcgtgc atatcgaaaa ccgtaaagat gcggaacgtg cggcaaaaat cctgcttgat
     2521 tacggtgtca aaaatgtcat tatcaaaggc ggacatttga acggcagcac aagcggacgc
     2581 tgcacggatt ggctgtttac gcaaaacgaa acgctggaat tggacagccc gcgctttccg
     2641 accgcccaca cgcacggcac gggctgcacg ttttccgcct gcattaccgc cgagttggca
     2701 aaaggcttag acgtttgcaa agccgtacag actgccaagg cctacatcac ggcggcaatc
     2761 tcaaaccctt tggaaatcgg cgcgggacac gggccggtca atcattgggc gtatcgggac
     2821 tagacgtaaa aatgccgtct gaacgccttt tcgggttcag acggcatcgt ccggcgcggc
     2881 ttatcctggt tttttcgcca gcatagttac aaatttaaac cgtatcgggt tgccgttttc
     2941 gtctttggcg tgcatcgcgc ccaattcttc tttatattcg accagctccc aatcccgata
     3001 ataatccttc agctcgccct ctttaaattt gaaggggaac ggcatcgggc aggggaaatc
     3061 cgccgtatcc attgccgata cgattaagtt gtacccgccc gccgccgtat gcgcctgcat
     3121 atcggcaatc acgtcgggca cgcgctgcgg catcaggaac atcatcacca ccgttgccac
     3181 aatataatca aactcgccct gaagggcggc ggcgttcaaa tcatattcca gcgtgcggac
     3241 gttcaaaccc tccgcctctg ccagttccgc cacgtttgcc aacgcggcgg gattgcaatc
     3301 ggcagcggtt acatcaaacc ccttcaaacc caggaacagc gcgttgcgcc cctgtccgca
     3361 gcccatatcc aacgccctgc ccgccggtac ggtatcctgt gccgccgcga ccgcagaatg
     3421 cgtggcactc atcccgtatt ttttgtgaaa atagtctgcc gccgcgcaat acagcgacaa
     3481 acggatttcg gcatcgtccg ttttcggttt gaccgaaaac acctgctgcg gcgcaaacac
     3541 gcaatcgccg ctttccgccg accaaacctc tgccgccccg tccggtgcac ggacttcgac
     3601 atcgccctgc aacacattca ggcagaccca ttccccttcc tcggacgaat aacccgacaa
     3661 caaaacttcc ggcaggtttt ccactttcca tacaggcatt tgtccgaaac aaaacaactc
     3721 gccactttga cccactatcc gctccttcat attcaaaaat aaagttgcac attatatgcc
     3781 tatttcaatc cgccgcaatc tttcagacgg cacgacgcac aaaccgctta taatcacgcc
     3841 ggacaccaca caaaggcacg atatggacac acccgtttac ctctacacgg acggcgcgtg
     3901 caaaggcaat cccggcgcgg gcggctgggg cgtattaatg cgctacggca gccgcgaaaa
     3961 agaacttttc ggcggcgaag cgcaaaccac caacaaccgc atggagctga ccgccgttat
     4021 cgaagggctg aaatcgctca aacgccgctg caccgtcatc atctgcaccg actcgcaata
     4081 cgtcaaaaac ggcatggaaa actggataca cggttggaag cgcaacggct ggaaaaccgc
     4141 cgccaaacag cccgtcaaaa acgacgactt gtggcaagaa ctcgacgctc tggtcgggca
     4201 gcatcaagtc agctggactt gggttaaagg acacgcggga cacgcggaaa acgaacgcgc
     4261 cgacgatttg gcaaaccgtg gcgcggcgca gttttcttga ttgccgcacc gacaaaaatg
     4321 ccgtctgaaa ccgctaatgg gcttcagacg gcatcgtcct ccaccgtcat tcccgcgcag
     4381 gcgggaatcc agaacgtcgg gcaacggcaa tattcaaaag ccgtctgaaa atttaaaagt
     4441 tctagattcc cgttttcacg ggaatgacga aaagttgcgg acagcagagg a
//
LOCUS       DBJGGI010000103         4228 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.103, whole genome shotgun sequence.
ACCESSION   DBJGGI010000103 DBJGGI010000000
VERSION     DBJGGI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.103"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            306..440
                     /locus_tag="ACSIGJ_002001"
     CDS             306..440
                     /locus_tag="ACSIGJ_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330791.1"
                     /translation="MPSERIFRSVLRLVGVSSDTVKRKFAGAGLSYAGKAAGESLYCL
                     "
     gene            556..1350
                     /locus_tag="ACSIGJ_002002"
     CDS             556..1350
                     /locus_tag="ACSIGJ_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HHQ1330792.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYDLPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAVSCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELVEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDTGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            1575..4178
                     /gene="pepN"
                     /locus_tag="ACSIGJ_002003"
     CDS             1575..4178
                     /gene="pepN"
                     /locus_tag="ACSIGJ_002003"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HHQ1330793.1"
                     /translation="MSKTVRYLKDYQTPAYRILETELHFDIAEPQTVVKSRLTVEPQR
                     AGEPLVLDGSAKLLSVKINGAAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEFSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDRFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRAGRA
                     VRRIENIRLLRQNQFPEDAGPTAHPVRPVSYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNVFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAAAFDYQGKRATEAVLLLT
                     EAEQIFLLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGT
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELDRQAAKQENQSYEYSPETADWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNCLLAQFADKFSDD
                     ALVMDKYFALIGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAQD
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQELQCIRAQEGLSK
                     DVGEIVGKILG"
BASE COUNT         1043 a         1129 c         1127 g          929 t
ORIGIN      
        1 ccttgtcctg atttttgtta atccactata aaattaaatt tgtttaaaaa cataaagttg
       61 taaacaagta tctcatataa gcctttttca ttaaacagat agtcagatat tttgtgctaa
      121 aaatttatat aatatttaaa ttaatatcaa gttataaaaa atatatggaa ttttattttg
      181 tttatttata attttaagca ataatctaaa tcaggcattt ttattcccct gttttaaaaa
      241 atatttggca aggtgtgaaa aaaggcgtac attccgctac acagaattac agatacagcg
      301 gagcaatgcc gtctgaaagg attttccggt cagtcttgcg gttggtcggg gtttcatcgg
      361 atacggtgaa acgaaagttt gccggcgcag ggttgagcta cgcgggtaaa gccgcaggcg
      421 aaagcctgta ttgtttgtga agcgtaaatc tctgatttga ggtattgggg caatcctgtg
      481 ggggttgcct ctttttttat ccgcctttta atgacacaat aggcgcaagt ctgtttttga
      541 atgcaaggta ctgccatgaa tacgattttg gctttcgata ttgaaaccgt acccgatgtg
      601 cagggtatcc gtacgttgta tgacttgccg tcttccctgc cggatgacga agtggtgctg
      661 ttcgcgcagc agaaacgccg cgcacagacc ggcggcgatt ttatgcagca ccacctccat
      721 caggttgtgg cggtttcctg ctgtatgcgc tgggggcagg acaaggttca tgtcggcacc
      781 atcggcgaga tggacgatgg cgaggaagtg gttatcgcca aatttttcga gttggtggaa
      841 aaacatacgc cgcaactggt cagttggaac gggggcgggt tcgatttgcc cgtactgcat
      901 taccgctccc tgatatacgg catcaacgcc gcgcgctatt gggatacggg cgacggcgat
      961 ttcggcgaca gccgcgattt caagtggaac aactacatca gccgttatca ccaacgccac
     1021 tgcgatttga tggatttgct cgcgctttac cagccgaggg cgaatgtgcc gctggacgat
     1081 atggcgaaac tgtgcggctt tccgggcaag ctgggtatgg acggcagcaa ggtgtgggag
     1141 gcgttccacg cgggcaggct gaaggaaatc cgcaattatt gcgaaaccga tgccgtgaat
     1201 acgtatttga tgtatctgcg tttctgtctg gtcagcggca gattcgacgc ggacgaatac
     1261 gaaatggaaa tcaagcggat cagaaactat ctctccgccc aaacagaaga caaaccgcat
     1321 tgggcagaat ttgtccaagc gtggaaatag aaacctgctg tccgaaggcg gtacgcttgt
     1381 gccgccgttt cggtttcaga cggcgtatgg tggattaaat tcaaaccggt acagccttgc
     1441 ctctccttgc cttactatct gtactgtctg cggcttcgtc gccttgtcct gatttttgtt
     1501 aatccgcttt aaaagctgta tccgtgccgt ctgaagcctt ttccagtcaa accaaacaat
     1561 attgaaagaa aaaaatgagc aaaaccgtcc gttatctgaa agattaccaa acgcctgcct
     1621 accgcattct tgaaaccgaa ctgcatttcg acattgccga accgcaaacc gtcgtgaagt
     1681 cgcgtttgac ggtcgagccg cagagggcgg gcgagccgct ggtgttggac ggttcggcaa
     1741 aactcttgtc cgtcaaaatc aacggcgcgg cggcggatta tgtgttggaa ggcgagacgc
     1801 tgacgattgc agacgtaccg tccgaacgct tcaccgtcga agtggaaacc gaaatcctgc
     1861 cggcggaaaa caaatcgctg atggggctgt atgcttccgg cggcaatctg tttacccagt
     1921 gcgagccgga gggcttccgc aaaatcacgt tctacatcga ccgtccggat gtgatgtcca
     1981 agttcacgac caccatcgtc gcggacaaaa aacgctatcc cgttttgctt tccaacggca
     2041 acaaaatcga cggcggcgag ttttcagacg gccgccattg ggtgaaatgg gaagacccgt
     2101 ttgccaaacc gagttatctg tttgctttgg tcgcgggcga tttggcggta acggaagacc
     2161 gtttcaccac catgagcggc agaaacgtca aaatcgagtt ttacaccacc gaagcggaca
     2221 agcccaaggt cggctttgcc gtggaatcgt tgaaaaacgc gatgaagtgg gacgaaacgc
     2281 gcttcgggtt ggaatatgac ttggatattt tcatggtcgt cgccgtaggc gatttcaata
     2341 tgggcgcgat ggaaaacaag ggtttgaaca tttttaacac caagttcgtc ctcgccgaca
     2401 gccgcaccgc caccgatacc gatttcgaag gcattgaatc cgtggtcgga cacgaatatt
     2461 tccacaactg gacgggcaac cgcgtaacct gccgcgactg gttccagctt tcgctgaagg
     2521 aagggctgac cgtgttccgc gaccaagagt tttccggcga ccgcgccggc cgcgccgtgc
     2581 gccgcatcga gaacatccgc ctgctgcgcc agaaccagtt ccccgaagac gcaggcccga
     2641 ccgcccatcc ggtgcgcccc gtcagctatg aggagatgaa caatttctac accatgaccg
     2701 tttatgaaaa aggcgcggaa gtggtgcgga tgtatcatac cctgctcggc gaagagggct
     2761 tccaaaaagg catgaagcta tatttccaac gccacgacgg acaggcagtg acctgcgacg
     2821 atttccgcgc ggcgatggcg gatgcgaacg gcatcaatct cgaccagttc gccttgtggt
     2881 acagccaggc gggcacgccc gttttggaag ccgaaggccg tctgaaaaac aatgttttcg
     2941 agttaaccat taaacaaacc gtgccgccca cgcccgatat ggcggacaaa cagccgatga
     3001 tgattcccgt caaagtcggg cttctgaacc gcaacggcga agcggcggca ttcgattatc
     3061 agggcaaacg cgcaaccgaa gccgtattgc tgctgaccga agccgaacag atcttcctgc
     3121 tcgaaggcgt aaccgaagcc gtcgttccct cgctgctgcg cgggttcagc gcgccggtgc
     3181 atctgaacta tccgtacagc gacgacgacc tgctgcttct gctcgcccat gacagcgacg
     3241 ccttcacgcg ctgggaagcc gcccaaacgc tctaccgtcg cgccgtcgcc gccaaccttg
     3301 ccgcactttc agacggcatc gggttgccga aacacgaaaa actgcttgcc gccgtcgaaa
     3361 aagtcatttc agacgacctc ttggacaacg ccttcaaagc cctgcttttg ggcgtgccgt
     3421 ccgaagccga actgtgggac ggcacggaaa acatcgaccc gctgcgctac catcaggcgc
     3481 gcgaagcctt gttggatacg cttgccgtcc gcttcctgcc gaaatggcac gaattggacc
     3541 gtcaggcggc gaagcaggaa aaccaaagtt acgaatacag ccccgaaacc gccgactggc
     3601 gcacgctgcg caacgtctgc cgcgccttcg tcctgcgcgc cgaccccgcg cacatcgaaa
     3661 ctgttgccga aaaatacggc gaaatggcgc aaaacatgac ccacgaatgg ggcatcctgt
     3721 ccgccgtcaa cggcaacgaa agcgatacgc gcaactgcct gctggcgcag tttgccgaca
     3781 agttttcaga cgacgcgctg gtgatggaca aatatttcgc ccttatcggc tcaagccgcc
     3841 gcagcgacac cctgcaacag gttcaaaccg ccttgcagca tccgaaattc agtctcgaaa
     3901 accccaacaa agcccgttcg ctcatcggca gcttcagccg caacgtcccg cattttcacg
     3961 cacaagacgg cagcggctac cgcttcatcg ccgacaaagt catcgaaatc gaccgcttca
     4021 acccgcaggt cgccgcccgc ctggtgcagg cgttcaacct ctgcaacaag ctcgagccgc
     4081 accgcaaaaa cttggtgaaa caagaattgc agtgcattcg ggcgcaggaa ggattgtcga
     4141 aagacgtggg cgaaatcgtc ggcaagattt tgggttgagg cagtcaaaca gaaaaaacaa
     4201 gggtctgtac cagattagca gatatgtt
//
LOCUS       DBJGGI010000104         4207 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.104, whole genome shotgun sequence.
ACCESSION   DBJGGI010000104 DBJGGI010000000
VERSION     DBJGGI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.104"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..228
                     /locus_tag="ACSIGJ_002004"
     CDS             <1..228
                     /locus_tag="ACSIGJ_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HHQ1330794.1"
                     /translation="KKTVKFQFPPEVVRVLAGMGNNLNQIAKALNTAAKVGTLGNVEA
                     LKATTELAALERSLNSLRDFLAKEKNGWQSQ"
     gene            213..842
                     /locus_tag="ACSIGJ_002005"
     CDS             213..842
                     /locus_tag="ACSIGJ_002005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HHQ1330795.1"
                     /translation="MAVPMIVQFFNRGKGGGSGPIDYLLGKDRDREEARLLRGDPEET
                     AALINSSDYAKKYTAGCLSFEESNIPAEQKHALMDSFEECIFAGLDKDQYNCLWVEHR
                     DKGRLELNFVIPNIELLSGKRLQPYYYAADRGRVDAWRTMQNLTHGYSDPDDPAKRQS
                     MTQAKDLPRNTQEAAQSITEHHRRLRSPSPIRQAKKPRRRAGNAGKGRF"
     gene            926..1567
                     /locus_tag="ACSIGJ_002006"
     CDS             926..1567
                     /locus_tag="ACSIGJ_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HHQ1330796.1"
                     /translation="MSKISDLAKTFEQTSRTEAASIEQQTKADLETLRKNINEALKQS
                     EQKITADINARQLRMSKAVFKPYLWSLLGISAAGLIVIAGLFIAIWSVKNELDDLKQQ
                     RAEAERTLDLLETKTKGLTLENCPVENSKATRVCVATEKRMLDALAELESNHAAIEQR
                     MMKALTHLGERLAELEQENTSLAQQLASLAAELERQSEIQQRQSEILNQLAKR"
     gene            1614..1868
                     /locus_tag="ACSIGJ_002007"
     CDS             1614..1868
                     /locus_tag="ACSIGJ_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cryptic plasmid protein A"
                     /protein_id="HHQ1330797.1"
                     /translation="MITENERDRRTAAWLIETYGAEAVAEAETRIAGARKPYPSNIAK
                     VLGASLPEALKRTENAAARQKLAGLRRMLDGKAVKTSQDL"
     gene            complement(1846..2124)
                     /locus_tag="ACSIGJ_002008"
     CDS             complement(1846..2124)
                     /locus_tag="ACSIGJ_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease VapD"
                     /protein_id="HHQ1330798.1"
                     /translation="MYAISFDLVVADTAQNHPKGISQAYADIGYTLRKFGFTRIQGSL
                     YTCQNEDMANLFSAINELKALPWFPSSVRDIRAFRIEQWSDFTSLVKS"
     gene            complement(2124..2309)
                     /locus_tag="ACSIGJ_002009"
     CDS             complement(2124..2309)
                     /locus_tag="ACSIGJ_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5397 family protein"
                     /protein_id="HHQ1330799.1"
                     /translation="MQTTIPTGSIRTFGDYGVMYIVGTPAEQLPDGDWLVNIELPESG
                     EHTQYKLSHIVQDPKAA"
     gene            complement(2312..2557)
                     /locus_tag="ACSIGJ_002010"
     CDS             complement(2312..2557)
                     /locus_tag="ACSIGJ_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330800.1"
                     /translation="MTRYAINRDALYSAFKDFLYSEINANPALKSATVDDLADIVLAK
                     KWRIFLPDGIKRTTAEKAAQRVLYMTNQPENQTQQEN"
     gene            complement(2554..2796)
                     /locus_tag="ACSIGJ_002011"
     CDS             complement(2554..2796)
                     /locus_tag="ACSIGJ_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HHQ1330801.1"
                     /translation="MAYPKLKTTKRDVTAKELAKRFGCSTRTVFRAWSQSRADYLAEN
                     SISRDKPWEHFGISRATWYRRGKPMPSETDNQSETA"
     gene            complement(2800..3669)
                     /locus_tag="ACSIGJ_002012"
     CDS             complement(2800..3669)
                     /locus_tag="ACSIGJ_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HHQ1330802.1"
                     /translation="MSQLYTQPDLFLQERIPHKPYCKDFKEAPMLVRSYAAAIKRRYI
                     QVNPPHLRVFMLFDLDYEGAGLAWEDNNLPMPAWAAINRENGGAHLAYALSAPVLTAE
                     YGGRQKALRYLAALEAAYKAKLRGDVGFVSLITKNPEHPHWLTLRGVPDAIRGYDLEY
                     LADFVDLDKFKPYIGRSNVEAVGLSRNCTVFNLVSRWAHKNVLAFKQQGYTVQGWLKE
                     VHYQCMRVNGDFPVPMWEKEVKCISKSIANWVWYKFDIAASNRRFSELQAHRNSLRKT
                     TINAGRTKIITEL"
BASE COUNT         1144 a         1039 c         1129 g          895 t
ORIGIN      
        1 aaaaaaaccg tcaaattcca attcccgccc gaagtcgtgc gcgtccttgc aggcatgggt
       61 aacaacctga accaaatagc caaggccctg aacaccgccg caaaggtcgg cacgttgggc
      121 aatgtggaag cactcaaggc gacgaccgag ctggcagcgt tggaacgttc cttaaattcc
      181 ctacgggatt ttttagccaa agaaaagaac ggatggcagt cccaatgatt gtgcagtttt
      241 tcaatagggg gaaaggcggc gggagtggtc cgatagacta tcttctaggc aaagaccgcg
      301 accgagaaga agccagatta ttacgcggcg accccgaaga aaccgccgcc ctgataaaca
      361 gcagcgatta cgccaagaaa tacaccgccg gctgcctgag ctttgaagaa agcaacatcc
      421 ccgccgaaca gaaacacgcc ctgatggaca gcttcgaaga gtgtattttt gcaggcttgg
      481 acaaagacca atacaactgc ctatgggtag aacaccgaga caaagggcgt ttggaactca
      541 acttcgtgat accgaacatc gagcttttga gcggaaagcg gttacagccc tactactacg
      601 ccgccgacag aggaagagtg gacgcatggc gcaccatgca gaacctgacg cacggataca
      661 gcgacccaga cgaccccgcc aaacggcaga gcatgaccca agccaaagac ctgccgagaa
      721 acacgcagga agccgcacag agcatcacag agcatcacag acggcttaga agccctagcc
      781 ctatcaggca agctaaaaag ccgcgcagac gtgctggaaa cgctggaaaa ggtaggtttt
      841 gaaatatcac gagcgaccat cagcagcatc agcatcaaga acccggaccc aaaagggcgc
      901 aacatccgac tgaaaggcgc actgtatgag caagatttcc gatttggcga agaccttcga
      961 gcagacatca cgcaccgaag ccgccagcat agagcaacaa acgaaagcag acttagagac
     1021 gttacggaaa aatatcaacg aggcattgaa gcaaagcgag cagaaaataa ccgccgatat
     1081 aaacgcccgg cagttacgca tgagcaaggc agtattcaag ccctatctgt ggagcttgct
     1141 aggtatatcg gcggcagggt tgatagtcat agcagggctg ttcatagcga tatggagcgt
     1201 caagaacgag ctggacgact tgaaacagca gagagccgaa gcagagcgca ccctagacct
     1261 gttggaaacc aagaccaaag gtttgacact ggaaaattgc ccagtcgaga acagcaaagc
     1321 aacgcgggta tgcgtagcga ccgagaagcg aatgctggac gcgttagcgg aattagagag
     1381 caatcacgca gcaatcgagc agcgaatgat gaaagcctta acgcacttgg gcgaaaggtt
     1441 ggcagagcta gagcaggaaa acacgagttt agcgcagcag ctagcgagct tggcagccga
     1501 gttagagcgg cagagcgaaa tacagcaacg gcagagcgaa atcttgaatc aactagccaa
     1561 acgataagcc aacgacacaa acgaacccaa agcaggggat ggggaatgag ccgatgatta
     1621 ccgagaacga acgcgacagg cgaacagccg catggctgat agagacctac ggggcagaag
     1681 ccgtagcgga agcagaaacc cgcattgcgg gcgcgagaaa gccctatccg agcaatatcg
     1741 ccaaagtatt gggggctagc ctacccgaag ccctaaaacg cacagaaaac gccgcagcgc
     1801 gccaaaaact ggcagggctg cggcggatgt tggacggtaa ggcagttaag acttcacaag
     1861 acttgtgaaa tcagaccact gctcaatgcg gaacgcccga atatcgcgga cagaagacgg
     1921 aaaccaaggc agagctttta gctcgttgat ggctgaaaac aggttagcca tatcttcgtt
     1981 ttggcaggtg tacaaacttc cctgaatgcg ggtaaagccg aatttccgca gggtgtatcc
     2041 aatatccgcg taggcttggg aaatgccttt cggatggttt tgagcggtat cggcaaccac
     2101 caaatcaaaa gaaatcgcgt acattaggca gcctttgggt cttgaacgat gtgggacagt
     2161 ttgtattgcg tgtgttcgcc gctttccggc agttcaatat ttacgagcca atcgccgtcc
     2221 ggaagctgtt cggcaggcgt gccgacgatg tacatcacgc cgtaatcgcc aaaggtgcgg
     2281 attgaaccgg tggggatagt ggtttgcatg gttagttctc ctgttgggtt tggttttcag
     2341 gctggtttgt catgtacaaa acccgttggg cagctttttc ggctgttgtg cgtttgatgc
     2401 cgtcaggcag aaaaattctc cattttttgg ctagaactat atctgcaaga tcatcaaccg
     2461 ttgcgctttt caaagcagga tttgcgttaa tttccgaata cagaaaatct ttgaatgccg
     2521 agtacagagc atcgcggttt attgcgtatc ttgtcatgct gtttcgcttt ggttgtcagt
     2581 ttcagacggc ataggcttgc cgcgcctgta ccaagtggcg cgggaaatgc cgaagtgttc
     2641 ccacggttta tcgcggctga tagagttttc ggctaggtag tcggcgcggg attgcgacca
     2701 tgcccgaaaa acggttcttg tggaacagcc gaagcgtttt gccagttctt tagccgtaac
     2761 gtctcgcttg gtggttttca gttttggata ggccatattt caaagctccg tgatgatttt
     2821 tgttctgcct gcattgatgg ttgttttccg caaactattc cgatgagcct gcaattccga
     2881 aaaccgtcga ttgctggctg caatatcaaa cttgtaccaa acccagttag cgattgattt
     2941 ggagatacat ttcacttctt tttcccacat cgggacaggg aaatccccat ttacccgcat
     3001 acactggtaa tgcacttctt tcagccagcc ttgcaccgtg tagccctgct gtttgaacgc
     3061 caacacgttt ttgtgcgccc aacggctcac aaggttaaac accgtgcaat ttctgcttaa
     3121 tccgaccgct tccacgttag agcgaccgat atagggctta aacttgtcta aatccacgaa
     3181 atccgcaaga tactccaaat cgtagcccct gattgcgtca ggaacgccgc gcagcgtcag
     3241 ccaatgcgga tgttcgggat ttttcgtaat cagcgataca aagcccacat caccgcgcaa
     3301 tttcgcctta tatgctgctt caagtgcagc aagatagcgc agggcttttt gtctcccacc
     3361 gtattccgcc gtcagcacag gcgcggaaag cgcataggca aggtgtgcgc cgccgttttc
     3421 cctgttgatt gccgcccaag caggcatagg cagattattg tcttcccaag ccaaccccgc
     3481 cccttcgtaa tccaagtcaa agagcataaa cacacgcaga tgcggcggat tgacttggat
     3541 gtagcggcgt ttgatggcgg cagcgtaaga gcgcaccagc ataggcgctt ctttgaaatc
     3601 tttgcagtat ggcttgtgtg ggatacgttc ttgcaagaag aggtcgggtt gggtgtataa
     3661 ttggctcatg ttgtatctcg aaacccccgt gcagattggc gtttggcggg ggttttgctt
     3721 tgtctaagat ttgcagattg tatgcttgtt tttaagatga tacaactatg tcaaaataac
     3781 cataatcaga taacagcccg ataggggttc ttatttcaaa attttccaat ccgcaattta
     3841 gcgaagccag caggcgaagc ggtaaagctt ggagcgcagc agcgcgacct aagccggcca
     3901 gcagggcggc gttttggggg aaacatgaaa ccagttccga cagggcggcg tgcgtgttct
     3961 tccctgagtt cttcatggag tatcggcgaa atgccgtgat gaaatgccgt ttttttgagc
     4021 agaaagcagt caaaaacagg ggtattttgc ccttttgaca ggttcgagtg ccgccgaaaa
     4081 gcgaacaaag caactcatca tccgagtcag cccgaccgag tttgagactt tgacccgaca
     4141 gaagacccat ccgaatttag cccgctacat tcgggagcgg gttttggaag atggcaaagc
     4201 atccgac
//
LOCUS       DBJGGI010000105         3993 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.105, whole genome shotgun sequence.
ACCESSION   DBJGGI010000105 DBJGGI010000000
VERSION     DBJGGI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.105"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..314
                     /locus_tag="ACSIGJ_002013"
     CDS             <1..314
                     /locus_tag="ACSIGJ_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330803.1"
                     /translation="QQFLESDFIDLKEDFLSEKVSIPNRKRIALVQDRLNNMTLDQRQ
                     ELLNYLAEYNNILKFNADGSRVEISTDVQLKHLLYGIDERYYTTALGKEKRLANSVQP
                     I"
     gene            381..1982
                     /locus_tag="ACSIGJ_002014"
     CDS             381..1982
                     /locus_tag="ACSIGJ_002014"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdM family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HHQ1330804.1"
                     /translation="MTEQHFTEQIKSLIDSLKTICANYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKKIRKEKPDEPIEFVNMDIDGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAACNADLFSVKTEGGAKIVLFERISQYITDEGRRDDFCRALISKLAGFSFEA
                     IFAQKFDFFATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMADILVPEEVRGQIRSVD
                     VYDPSAGSGTLLMNVAHVIGEDKCMIYTQDISQKSSNLLRLNLSLNNLVHSLNNVVQG
                     NTILSPAHKDASGCLKKFDFIVSNPPFKLDFSDFRDRLESDENHERFFAGIPKIKPTK
                     KEKMEIYQLFIQHILFSLKENGKAAIVLPTGFITAKSGIDKKIREYLVENKMLAGVVS
                     MPSNIFATTGTNVSILFIDKVNKDKVVLIDASGLGEKISINDNQKTVLSHEEEQKICH
                     TFTHKQAVEDFSVVVGYDEIKAKNHSLSAGQYFEVKIDYVDISADEFVQKIAGFSADL
                     DKLFAESAELEKEIKDRLAMLKFNS"
     gene            2014..2130
                     /locus_tag="ACSIGJ_002015"
     CDS             2014..2130
                     /locus_tag="ACSIGJ_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HHQ1330805.1"
                     /translation="MPSESLSDGILFGLENTYAVLSPKERIQKTKATGIHRK"
     gene            complement(2293..3630)
                     /gene="ribBA"
                     /locus_tag="ACSIGJ_002016"
     CDS             complement(2293..3630)
                     /gene="ribBA"
                     /locus_tag="ACSIGJ_002016"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HHQ1330806.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQNRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTGRPPGHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAVNFMIKHARGLVCLPMEGAMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPADIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGDAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERVQQAESGVVILLHHTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLHVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(3843..3971)
                     /locus_tag="ACSIGJ_002017"
                     /pseudo
     CDS             complement(3843..3971)
                     /locus_tag="ACSIGJ_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         1044 a          845 c         1065 g         1039 t
ORIGIN      
        1 tacagcaatt cttagaaagc gattttattg atttaaaaga agatttttta agtgaaaaag
       61 tatctattcc caataggaaa agaatagctt tagttcaaga tagattgaat aatatgacgc
      121 tagatcaaag acaagaacta ttaaattacc ttgctgaata taataatatc ttgaaattta
      181 acgcagatgg ctctcgtgta gaaatttcta ctgatgtaca gctgaaacat ttgttatatg
      241 gtattgatga acgttattac acaacagcat taggaaaaga gaaaaggttg gcaaatagtg
      301 ttcaacctat ttaatctgcc agcaaaccaa ctaatgctaa acaaaattta agcgacgcta
      361 gcaacaaacc gagacattcc atgaccgaac aacactttac cgaacaaatc aaatccctaa
      421 tcgacagctt aaaaaccatc tgcgccaact atggtttggg caatgacggc aacgaattta
      481 aaatcatcag tcaggcgttt ttgtataaat tcctaaacga caaatacgat tttgaagtta
      541 aaaaaattcg caaagaaaag cccgacgagc cgatcgagtt cgtcaatatg gatattgatg
      601 gaaaaacagc ggttttaaag cccgaacatt ccatcaagta tttatcagag cggcaaaacg
      661 gcgcggattt tgccaaactc tttgacgata cgctcaccga tattgccgcc tgtaatgcgg
      721 atttgttttc ggtgaaaacc gaaggcgggg cgaaaatcgt gttgttcgag cgtatcagtc
      781 agtacattac cgacgaaggc cgacgcgacg atttttgccg cgcgctgatt tccaagctgg
      841 cgggtttcag cttcgaggcg atttttgcgc aaaaattcga ctttttcgcc acgatttttg
      901 aatacctgat taaagactac aacagtaatt ccggcggcaa atatgccgaa tactacacgc
      961 cgcacgcggt tgcgcggatt atggcggata ttttggtgcc ggaggaggtg cgcgggcaaa
     1021 tccgcagcgt ggacgtttac gacccgtcgg ccggttcggg aacgctgttg atgaatgtcg
     1081 cccatgtcat cggcgaagat aaatgcatga tttatactca ggatatttcg cagaaatcgt
     1141 ccaatttgtt gcggctgaat ctgagtttga acaatctggt gcattcgctg aataatgtgg
     1201 ttcagggcaa tacgattttg tcgcccgccc acaaagacgc ttcaggctgt ctgaaaaaat
     1261 ttgatttcat tgtttccaat ccgccgttta aattggattt cagcgatttc cgcgatcggc
     1321 tggaaagcga tgaaaaccac gaacgctttt ttgctggtat cccgaaaatc aagccaacaa
     1381 agaaagagaa gatggaaatc tatcagcttt ttatccagca tattctgttc agcctgaaag
     1441 aaaacggcaa ggcagcgatt gtattaccga ccggctttat cacggcaaaa tcgggcatcg
     1501 ataaaaaaat tcgcgaatat ctggtggaaa acaaaatgct cgccggcgtg gtgtctatgc
     1561 cttccaatat tttcgctacg acgggaacca atgtttctat cttgtttatt gataaagtca
     1621 ataaggataa agttgtatta atcgatgctt ccggtttagg tgaaaaaatt agtattaatg
     1681 acaaccaaaa aactgtactt tcccacgaag aagaacaaaa aatctgccat accttcacgc
     1741 acaaacaggc agtagaagat ttcagcgtgg tggtcggcta tgatgaaatc aaagcgaaga
     1801 atcacagctt gtcggcgggg cagtattttg aggtaaaaat tgattatgtg gatatatccg
     1861 ccgacgaatt tgtgcaaaaa atagcgggat tttcagcgga tttggataaa cttttcgccg
     1921 aatctgccga attggaaaag gagattaagg atagattggc gatgttgaaa ttcaattcat
     1981 aaataccgat gaatgaaacc ggtcaaactt aaaatgccgt ctgaaagcct ttcagacggc
     2041 attttgtttg gtttggaaaa tacttatgcc gttctttccc cgaaagagag aatccaaaaa
     2101 accaaagcca caggaattca tcggaaatga cacaaaaacc cgacgaaccg gattcccgcc
     2161 tgcgcgggaa tgacgaatta gaagttaccc gaaatccgaa aaaagcaaaa tccaacccaa
     2221 cagaccgggt tttcgtttgt acggaaataa tgcaataaat gaagcaaata taaagcattt
     2281 gaatttacta tattattttt ccgcttcttc aaagccgacg acttcgaggc cgaagccggt
     2341 gaggccggtg aaagatgacg gttggccgag gacgcgcagt tttttgacgt ggaggccggc
     2401 gaggatttgc gcgccgatgc cgtagctttt gctgtcccat ttgtaggctt ggtttgcgcc
     2461 tttgggtagg gtgcggtcga gcagggatgc gccgtcttcg gtgtggtgca ggaggatgac
     2521 gacgccgctt tcggcctgtt ggacgcgctc aagggctttg ggcagcgacc aggaatggcg
     2581 cggattggct tggatgaagt ccatcacgct gaagggttcg tggacgcgga cgagggtttc
     2641 ggtgtcggcg gagggtgtgc ctttgacgag ggcgaggtgg gtttcgccgg agagtttgtc
     2701 gacgtaaacg tgttgttgga actcgcccca cggagtttgt acgggcgcgt cgcccatatc
     2761 ttcgagcagg ctttcggtgc ggctgcggta ttcgatgagg tcggcaatcg tgccgatttt
     2821 gagcttgtgt tcttcggcga atttcatcag ttcgggcata cgcgccatcg tgccgtcgtc
     2881 gttgatgatt tcgcagatga cggcggcggg aatcagcccg ttcatttgcg ccaagtcgac
     2941 gccggcttcg gtgtgtccgg cacggacgag tacgccgcct ttttgggcgc gaagcgggaa
     3001 gatatgaccg ggttggacga tgtctgcggg tttggcggtc ggggaaacgg cggtttgaat
     3061 agtcagggcg cggtcggcgg cggaaatgcc ggtggtaatg ccgtgcgcgg cttcgatgga
     3121 aacggtaaag ttggtaccgt attgcgcgcc gtttttttgg gtcatcatcg gcagaccgag
     3181 tttttcgacc attgcacctt ccataggcag gcagactaag ccgcgcgcgt gtttgatcat
     3241 aaagttgacg gcttcggggg taacgaattg cgctgccatc agcaggtcgc cttcgttttc
     3301 gcggtcttcg gcatcggtga tgatgaccat tttaccggct ttgatgtcgg ctaggatttc
     3361 ggggatgggg gaaatatggg acatggtgtg ttttcctgtg tgtccgggcg gtcggccggt
     3421 gtgttcggta tcgagccaga aggcgggcat ttttgccgcc tgttcgattt tgcgggcttt
     3481 tttctcgccg aaggatttgt ttttcaaaag ggcggagagt tcgcctgtgt tgagggcaac
     3541 ggcttcggca aaacggtttt gcagtccgcc gtagtgttcc gctatccatt gccgcaggtt
     3601 gcggcggcgc aagccggctg tgtcggtcat cgttttatcc tttgtagaaa tcggatgtag
     3661 cggattgtaa gcgcgtattt actaaaaagc aaatttgttt ttcgccaaat cggattattt
     3721 gccttttggt aaacagatgc cgtttgaagc cggattcgtg gcttcagacg gcattgccgc
     3781 aaccccgccg gcggggcggc tgtttcttcc cgcacgcttc tgattatggg gtttccggag
     3841 ggttattccg aaaaaaaatt ctgataaaaa gccttgattg ccgggtacgg cataggcggt
     3901 acgagcaggc gcaataccgg caatcctatg atgaatgccg ccttcccgat aaaaaccgga
     3961 cggattgccc tttactttct acaagtaaca ggg
//
LOCUS       DBJGGI010000106         3953 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.106, whole genome shotgun sequence.
ACCESSION   DBJGGI010000106 DBJGGI010000000
VERSION     DBJGGI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.106"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            486..2044
                     /locus_tag="ACSIGJ_002018"
                     /pseudo
     CDS             486..2044
                     /locus_tag="ACSIGJ_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2027..2332)
                     /locus_tag="ACSIGJ_002019"
     CDS             complement(2027..2332)
                     /locus_tag="ACSIGJ_002019"
                     /inference="COORDINATES: protein motif:HMM:NF014299.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330807.1"
                     /translation="MDPIVGLARARGRGELDRIEQMDLDFFHRTRARYLELVKDNPKA
                     VVINAEQTIELVQAEIESAVKIGGNYTKNDRTFKIIKSAVNFPADFILLQFNLKRPL"
     gene            complement(2373..3740)
                     /locus_tag="ACSIGJ_002020"
     CDS             complement(2373..3740)
                     /locus_tag="ACSIGJ_002020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HHQ1330808.1"
                     /translation="MPDLHTEFSRLLPADEIAEPSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKLPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFPPEILSNEVYRYEN
                     DINSTVYRNVLARNGTIAAEHGIGIIKKQWLPAVRTPSEIALMKSIKQHLDPYNIMNP
                     GKLLP"
BASE COUNT         1127 a          833 c          950 g         1043 t
ORIGIN      
        1 gaaaatttat tatgaaactc gaagcaagca gaagtttaaa aaatcattta ttataagtct
       61 atttttttct attctttata cctctccgct tttggctgtt gattacgttt acgacaattc
      121 taaattaaca aatgagcaaa ttgatcgctt aaaaaaaact tagagataaa agcagcgaat
      181 actggaaaca agaaacttat cttttaaaac cgacgaatgg tagctctcag aactctctca
      241 aagttcccgc actatcctct aaaacacacc aattcgaaaa catcaacaac tcaaaaaaga
      301 tttcttttta tgacaaggaa tacactgaag attaccttgt tggcttcgcc cgaggtttcg
      361 gggttgaaaa aagaaatggg gaagaagaaa aaccactccg gcaatatttt aaggactgtg
      421 taaatactga gaattccaat aatgataagt gtgaacaacc atccttagga agctatgccc
      481 cgatacgcat aaagagcgat attttcgcct tagcatcaca aataaaaaat agccatatca
      541 acagcgaaat tttatctgta ggtaattata tcgaatggct tcgcttcgcc caacacttaa
      601 tcagctttct agttggcaag agcatctcta ttctgatgta gatccattcc actatattga
      661 ggttacagac aactcgcacg ttatcgggca aacgattagt ttggatgaat tccacttaga
      721 aaattctctc tgggaacccc gttgggattc tgatgttggg gagttaaaaa caacgaatgc
      781 agacatccgt tttaatacta aaagtgaaag tttactcgta aaagaaaatt atgctggcgg
      841 agctcgtttt cgttttgctt acgatctaaa agataaagtc cctgaaatac cagttttaac
      901 ttttgaaaaa aatataactg gcacatcaga tattattttt gagggaaaag cgttggataa
      961 tttgaaacac ctagacgggc atcaaattgt caaagtaaat gacaccgcag ataaagatgc
     1021 ctttagactt tccagtaaat accgaaaggg aatttatacg ctctctttac aacaacgccc
     1081 agagggcttt tttaccaaag tgcaagaacg cgatgatatt gcgatttatg cacaacaggc
     1141 tcaagccgcc aataccttat tcgccttgcg tttgaacgac aaaaacagca atatttttga
     1201 ccgcacttta ccacgcaaag gcttgtggtt gcgtgtgatt gacggacatt ccaaccaatg
     1261 ggtgcaaggc aaaacagcac ctgtagaagg ctatcgaaaa ggtgtgcaac tcggcggcga
     1321 ggtgttcaca tggcaaaacg aaagtaatca actttctatt ggcttaatgg gcggacaagc
     1381 agaacaacgc agcactttcc gcaatccgga tacagacaat cttacaacgg gaaatgtgaa
     1441 aggctttggt gcgggcgttt acgccacttg gcatcagctt caggacaaac agacaggtgc
     1501 gtatgttgat agctggatgc aatatcaacg tttccgccac cgcattaaca ctgaatatgc
     1561 tacagaacgt tttacttcaa aaggtattac tgcctcaatt gaagcgggtc acaatgcgtt
     1621 attggcggaa cacttcacta aaaagggcaa cagccttcgt gtttacctac aaccacaggc
     1681 gcaattgact tatttggggg taaacggaaa attcagcgat agcgaaaatg cccaagtgaa
     1741 tttgcttggc tcccgccaat tacaaagccg agtgggcgtt caagctaaag cgcaatttgc
     1801 tttcactaat ggcgtcactt tccaaccatt tgttgctgtc aattcaatct accaacaaaa
     1861 acctttcggt gtggaaatag acggggatcg tcgagtgata aacaataaga cggtaattga
     1921 aactcaatta ggtgtagcag cgaaaatcaa atctcactta accttgcaag cctcattcaa
     1981 ccgccaaaca agcaaacatc atcacgctaa acaaggtgcg ttgaatttac agtggacgtt
     2041 ttaagttaaa ttgtaacaaa ataaaatcgg cagggaaatt cactgccgat tttattattt
     2101 taaaagtgcg gtcatttttc gtgtaatttc caccaatttt taccgcactt tcaatttcag
     2161 cttgaacaag ttcaatagtc tgctctgcat taattacgac tgcttttgga ttatctttta
     2221 ctaattctaa atagcgtgct cgagtgcgat ggaaaaaatc taaatccatt tgctcaatac
     2281 ggtctaactc gccacgtcca cgagctcgcg ctaaaccgac aatcggatct attgtccttt
     2341 tctttgttgt gtatgcaaat cagaaatgcc ggttacggaa gcagtttgcc aggattcata
     2401 atgttatatg gatcaaggtg ttgtttgatg cttttcatca gggcaatttc agagggcgtg
     2461 cggacagcgg gcagccattg ttttttgatg atacctatgc cgtgttcggc ggcaatcgtg
     2521 ccgttgcggg caaggacgtt gcgatagact gtgctgttga tgtcgttttc gtaacggtaa
     2581 acttcattgc tgaggatttc gggcggaaaa gtattgtagt gcaggctgcc gtcgcccaga
     2641 tgtccgaagc agacgatttg tatgccttta aaattctgtt ccaaatcttt ggcgcaccgg
     2701 cggacaaagt cggcaacgcg cccgatagga acggcaatat cgtgtttgat gctggtgccc
     2761 agtttgcgtt gggaggcgga gatgttttcg cgcaacgccc acatatggat acgttcttgt
     2821 tcgctttgcg ccaatacgct gtcggtaaat ccttttttat aaagaaattc gacaagccga
     2881 tcatcaagat tgctgtcggg taatgagtcg gtcaactcaa gtaaaatatg ccattctgaa
     2941 tgtgtcggca gggggagttt gctgaattcg gaagacaact cggcggcaaa acggccgatc
     3001 agttcaaaac tgcataagcg ttcggcaaag tgcgcttggg tttcggtcag caggcggacg
     3061 gcggattcga tgtcgggaat gccgacccat gccgttgcct tgtccgaggg acgggcaaaa
     3121 agcttgagcg tggcggcagt gatgatgccc agtgtgcctt cgctgccgat aaacagatgg
     3181 cgcaggtcgt agccggtggt gtttttatgc aggggatgga gatgggaaac cagttcgccg
     3241 ttggggagga cgacttccaa accgataacc aggtcgcgca tcgtgccgta acgcaatacg
     3301 ttcaaacctc cggcattgca ggcgatgttg ccgccgattt ggcacgagcc ttcgctggcg
     3361 agactgagtg ggaacagcct gtttgaggct tcggctgcct gttggacggt ttggagtacg
     3421 gaacctgctt cgacggttat gcagttgtct gacaaattga tgctgcggat gcggttgagt
     3481 ttggaaaggt tcagcaatac gccgttttcc gatactgccg cgccgcacaa accggtattg
     3541 ccgccttgcg gcgtaaccgg aatgcggtgt tcgtaacaaa aacgcataat ggtttgcacg
     3601 ctttcaacgc tgcgcggctg caaaatgatg tctggtgcag acgtaaagcg gttgcgctga
     3661 tcttttaaaa gcgtcggaga aggttcggca atttcatctg ccggcaggag tcgggaaaat
     3721 tcggtatgaa ggtcaggcat gtttgtcgga agggtaggga gaagattgag gaagggaatc
     3781 tgatgccgtc tgaaaaatca aaaaagcagg atactgaacg tatcctgctt ttttgcaagc
     3841 ggaaaatgct tattttgcag cttcggcagc aggagcttca gcggcaggtg cttcggtagc
     3901 agcagcttcg gcggcaggtg cttcggtagc agcagcttcg gcggcaggag ctt
//
LOCUS       DBJGGI010000107         3840 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.107, whole genome shotgun sequence.
ACCESSION   DBJGGI010000107 DBJGGI010000000
VERSION     DBJGGI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.107"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(<1..825)
                     /locus_tag="ACSIGJ_002021"
     CDS             complement(<1..825)
                     /locus_tag="ACSIGJ_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HHQ1330809.1"
                     /translation="MDTKEILGYAAGSIGCAILAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLAMSVLCLGLDQAYIREYNAAADKDILFKALFLPPLLSAAAIAAFLLSHPALPSE
                     ILFSLDDAAAGIGLVLFGLSLLPIRFLLLVLRMEGRALAFSSAQLVPKSALLLLLLTV
                     WLLHFPARTAVLTSVYALANLAAAAFLLFQNRCRLKAVRRAPFSPAVLHRGLRYGIPL
                     ALDSLAYWGLTSADRLFLKKYAGLEQLGIYSMGISFGGAALLFQSIFSTVWTPYIFR"
     gene            complement(1020..2129)
                     /gene="ribD"
                     /locus_tag="ACSIGJ_002022"
     CDS             complement(1020..2129)
                     /gene="ribD"
                     /locus_tag="ACSIGJ_002022"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HHQ1330810.1"
                     /translation="MFSDTNISMMENALRLAALGRFSTSPNPRVGCVIAHGRQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAKGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPPVAGKGLVLLKAAGIKTECGLLENKARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEEARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPPNSHLVTDGQSPTYIATLERDEDKLRPYREHAHIRILMPSETADGK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGGGKDLFSLP
                     ENRAALSAPPLWTPVSTEILGHDIKTVFRKNGNAF"
     gene            complement(2161..2625)
                     /locus_tag="ACSIGJ_002023"
     CDS             complement(2161..2625)
                     /locus_tag="ACSIGJ_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HHQ1330811.1"
                     /translation="MKCPFCSHPDTRVADSRLMEERNTVRRRRHCPGCGKRFGTLETV
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     ISSSELVEIVLEELFGQNTEAAVRFAALHKRFDSPEHFASWLAQAVKTGGKT"
     gene            complement(2654..3496)
                     /locus_tag="ACSIGJ_002024"
     CDS             complement(2654..3496)
                     /locus_tag="ACSIGJ_002024"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HHQ1330812.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLVDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGISLFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     DDRVAVISTLPLTRDESWSQLAVNELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
BASE COUNT          801 a          984 c         1257 g          798 t
ORIGIN      
        1 gcggaaaata tacggtgtcc aaaccgttga aaagatgctt tggaacaata atgccgcccc
       61 gccgaacgaa atacccatcg aataaatgcc gagctgttcc aggccggcat attttttcag
      121 gaacaaacgg tcggcggatg tcagtcccca ataggcaagg ctgtccagtg cgagcggtat
      181 gccgtagcgc agcccgcgat gcaggacggc gggcgaaaac ggtgcgcgcc ggacggcctt
      241 cagacggcat cggttttgaa acagcaaaaa ggcggcggcg gcaaggtttg ccagcgcgta
      301 aacggaggtc aggacggcgg tgcgcgccgg aaagtgcagc agccagaccg tcagcagcaa
      361 cagcagcagg gcggacttgg gtacgagttg cgcggacgaa aaggcaaggg cgcgcccttc
      421 catacgcaaa accagtaaga gaaagcggat gggcagcaag ctcagtccaa acagcaccag
      481 cccgatgccg gcggcggcat cgtcgagcga aaacaggatt tcagacggca gggccgggtg
      541 ggaaagcagg aaggcggcta tcgcggcggc agacagcagc ggcggcagga acagggcttt
      601 gaacaaaatg tctttgtcgg cggcggcatt gtattcgcgg atatatgcct gatccagccc
      661 gaggcacaac accgacatcg ccagtcccgc cgccgtctgc atcaacacga tgcgcccgat
      721 gtcgtcggcg gggaaatacc acgacagcag cggcaggatg atgacggcta aaatcgcgca
      781 gccaatcgat cctgccgcgt agccgaggat ttcttttgtg tccatttttg atgtccggtc
      841 ggcggcggga tgctgcctgt gccgtctgaa gcctttcttg atcggaattt gacggctttc
      901 aggccgtcgc ggctgccggc ggggtgcggc agcccgggtt gcgcttttcc gggcgggcgg
      961 cggtctgaac gggccgtttt ttatcggcgt tattatatag tgaaacggcg gcaaaccctt
     1021 taaaaggcgt tgccgttttt ccggaacacg gttttgatgt cgtgtccgag gatttcggtg
     1081 gaaacgggtg tccataatgg cggcgcggaa agggcggcgc ggttttcggg cagggaaaac
     1141 aggtctttgc cgccgccgag gattttgggc gaacggtaca gcacgatttc gtccgccaaa
     1201 ttttccgcca aaaatgcgga tgtgagttcg gagcctgctt cgaccatgat ttcgccgaaa
     1261 ccttcgtcgg caaggaggcg catcagatgg tgcaggtcga ttttgccgtc tgccgtttca
     1321 gacggcatca ggatgcggat gtgtgcgtgt tcccgatagg ggcggagttt gtcttcatcg
     1381 cgttccaagg tggcgatgta ggtcggagat tgtccgtcgg taaccaaatg gctgttcggg
     1441 ggcaggcgca ggcggctgtc taaaacgatg cgtgcgggtt ggcgcaaagt tggaaaagcg
     1501 cggacgttga gccggggatt gtctgccaac accgtgccga tgccggtcag caccgcgcag
     1561 ctttcggcac gcaaaacctg tacgtcggca cgcgcttcct cgccggtaat ccaaaagctg
     1621 ctgccgtctg aaagggcggt tttgccgtcc agcgaaacgg cgcatttgag gcggacaaag
     1681 gggcggcggc gttcgatgcg cgataaaaat ccccggttga gttcgcgtgc tttgttttca
     1741 agtagtccgc attccgtctt gatgcccgct gctttgagca ggacaagccc tttgcctgca
     1801 accggcgggt tggggtcgcg catggcggcg acgacgcgtg ttacgccgga acggagcagg
     1861 gcttcggcgc agggcggtgt gcgtccgtaa tggctgcacg gttcgagggt aacaaaggcg
     1921 gtcgcgcctt ttgccatttc ccccgcctga cgcagggcgt ggacttcggc atggggttcg
     1981 cccgctttga cgtggaagcc ctgtccgaca atttgtctgc cgtgtgcgat aacgcagccg
     2041 acgcgcgggt tgggtgaagt ggaaaaacgc cccaaagcgg caagtcggag ggcgttttcc
     2101 atcatggata tatttgtgtc cgaaaacata gggataccgt atcagtatgg gttgggggaa
     2161 tcaggttttg ccgcctgttt tgacggcttg cgccagccac gaggcgaaat gttccgggct
     2221 gtcgaagcgt ttgtgcaggg cggcgaaacg gacggcggct tccgtgtttt gcccgaacag
     2281 ctcctccagc acgatttcga caagttcgga tgaggatatg tcgcgctgac ccgaagtgta
     2341 gagcctgtgt tccgtcaggc ggacggtttc gtcaatctgt tcgggtgtca gggcggattt
     2401 tcgggcggcg gcggtcaggt cgttgcggag gcgttgtgca ttgaagggcg aacgtttctt
     2461 gtccggaccg atgacggcgg gcattttgag ttcgacggtt tcgagcgtgc cgaagcgttt
     2521 gccgcagccg gggcagtggc ggcggcggcg gacggtattg cgctcttcca tcaggcggga
     2581 atcggcaact cgggtgtctg ggtggctgca aaacgggcat ttcatggtgt ttcttcctga
     2641 tgccgtctga acgtcaaacc gatacgccgg cggcgcgggc gatttccagg ccttcttcgg
     2701 cactcatata gacgggattt tcgggacggt cgtgccggac gatgttgcct tcgcggaaca
     2761 tgaccagttc gttcacggca agttgggacc acgattcatc gcgagtcagc ggcagggtgg
     2821 agataacggc gacgcggtcg tccggcgtgg ttacttcggc aaaatcgacc attacatcgt
     2881 cgtcgagcag gcgcgctttg ccgaacgggg cttggcggac gatgtagtgc agcagcgtgc
     2941 tggcgtgggc aaacagggaa atgccgtctg aaagcatgaa gttaaacagc ccgaacttgc
     3001 ggatttcgtg cgtcagcccc gcaatcgcgt caaacagcgt gtcgtcgtcg ggacgggcgg
     3061 caaagcgggt gcgcaggcgg ttgaggatgt ggcagaacgc gcgttcggaa tcggttgtgc
     3121 cgacggggtg gaaaaattct ccttgttcgg ggaaaaaatc gaccaaatgc ccgttgtggg
     3181 caaacagcca gtagccgccc cacatttcgc gcataaaggg atgggtgttc gccagcgagg
     3241 tttgaccttg cgaggctttg cggatgtggg cgacgacgtt ttccgattta atctggtagg
     3301 cgcgcacgag gtcggcgacg ggggaatttg cactcggctt gtcgtcatgg aacaggcgca
     3361 cgcctttgcc ttcgaaaaag ccgatgccga aaccgtcggc gtgatggtcg gtaatgccgc
     3421 ccctgcggcg gaagccttca aaggaaaaca taatatcggt cggcgtattg cagttcatgc
     3481 ccagcagttg acacatagtt tgtccaaatg attcagatgg tcgcaagtat tcggattata
     3541 ccccgaactg aaaatgccgt ctgaaatacg gcttgttccc cattattccc gcgaaaacag
     3601 aaaacaaaga cggaaactta agattccgtc attcccgcgc aggcgggaat ccgacttgtc
     3661 cggtttcggt tgtttttcgt tccgtaactt ttgagccgtc attcccgcgc aggcgggaat
     3721 ctagaatttc aatgcctcaa gaatttatcg gaaaaaacca aaacccttcc gccgtcattc
     3781 ccacgaaagt gggaatctag aaatgaaaag caacaggaat ttatcggaaa tgaccgaaac
//
LOCUS       DBJGGI010000108         3625 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.108, whole genome shotgun sequence.
ACCESSION   DBJGGI010000108 DBJGGI010000000
VERSION     DBJGGI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.108"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..449
                     /locus_tag="ACSIGJ_002025"
     CDS             <1..449
                     /locus_tag="ACSIGJ_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HHQ1330813.1"
                     /translation="SLRRGAIYKVWQFVEDALRAVVPADSFEPTAQKLKLFKAGAATI
                     LFYEDQNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGAGANLQHYNPLP
                     DVAIAKAWNIPENWLLRAQMVIGGIEGAAGEKVFEPVAERLKVFGA"
     gene            complement(504..959)
                     /locus_tag="ACSIGJ_002026"
     CDS             complement(504..959)
                     /locus_tag="ACSIGJ_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HHQ1330814.1"
                     /translation="MNRRQFLGSAAAVSLASAASFARAHGHADYHHHHDMQPAAASAY
                     TAVRQTAAHCLDAGQVCLTHCLSLLTQGDTSMSDCAVAVRQMLALCGAVHDLAAQNSP
                     LTRDAAKVCLEACKQCAKACKEHSAHHAECKACYESCLDCIKECEKLAA"
     gene            complement(1215..2585)
                     /gene="ffh"
                     /locus_tag="ACSIGJ_002027"
     CDS             complement(1215..2585)
                     /gene="ffh"
                     /locus_tag="ACSIGJ_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HHQ1330815.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNQALVELMGKENKTLDLSVSPPAI
                     ILMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAVVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLNGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKSGRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRLAKGMKGMK
                     GMFPGL"
     gene            2807..3613
                     /locus_tag="ACSIGJ_002028"
     CDS             2807..3613
                     /locus_tag="ACSIGJ_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly family protein"
                     /protein_id="HHQ1330816.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDFPFMLHIGTSLLAYGLFGIATLLSVLSLLLHRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
BASE COUNT          794 a          900 c          975 g          956 t
ORIGIN      
        1 cgtccctaag aaggggcgca atatataagg tgtggcaatt tgtcgaagac gcgctgcgtg
       61 ccgtcgtgcc tgccgacagt tttgaaccga ccgcgcaaaa attgaagctg tttaaggcgg
      121 gcgcggcaac cattttgttt tatgaagatc aaaatgtcgt caaaggtttg caggagcagt
      181 tccctgctta tgccgccaac tttcccgttt gggcggacca ggcgaacgct atggtacagt
      241 atgccgtctg gacgacactt gccgcggtcg gtgcaggtgc aaatctgcaa cattacaacc
      301 ccttgcccga tgtggcgatt gctaaagcgt ggaatattcc cgaaaactgg ctgttgcgcg
      361 cgcaaatggt tatcggtggt attgaagggg cggcaggtga aaaggtcttt gaacccgttg
      421 cggaacgttt gaaagtgttc ggcgcataat ttcgcggtca aaaaaatgcc gtctgaaccc
      481 cgttcagacg gcatttttca gtatcaggcg gcgagttttt cgcattcttt gatacagtcg
      541 aggcaggact cgtaacaggc tttgcattcc gcatggtggg cggagtgttc tttacaggct
      601 ttggcacact gtttgcacgc ttcgaggcac acttttgccg cgtcgcgtgt cagaggggaa
      661 ttttgtgcgg caaggtcgtg caccgcgccg cataaggcaa gcatctggcg cacggcaacc
      721 gcacagtcgg acatagacgt gtcgccctga gtgagcaggg acaggcagtg ggtcaggcaa
      781 acctgtccgg catccagaca gtgtgcggca gtttggcgga cggcggtgta ggcggatgcg
      841 gcggcaggct gcatatcgtg atgatggtgg tagtcggcgt gtccgtgcgc acgcgcgaaa
      901 gaggcggcgg aagccaaaga gacggcagcg gcgctgccca aaaattgacg acggttcatg
      961 attttttcct ttatgtttca acatgcgggt ccggcataag gtacacactt accatactgc
     1021 cctggccgga tcggtacgat tttctgaata actgcttgat tttgcaggtg tgaaattgtc
     1081 gcacgagcag gcatgtttta gctattcgga aattcgagga agggtgcatc aacctgtttt
     1141 gaaatgatgc gcagacatgg taacagacaa aaagccgtct gaaaatcgtt ttagacggct
     1201 tttgttttca aacattaaag accgggaaac attcccttca ttcccttcat gcctttggcc
     1261 aagcgcatca gtttgcccaa accgttgccg ctgaacatct tcatcatctg ctgcatttgt
     1321 tcaaactgtt tgagcaattt gttcacttcc tgcacggtcg tgcccgcgcc catcgcaata
     1381 cggcgtttgc ggccggattt gagcagggca gggttggcgc gttctttagg ggtcatcgag
     1441 ttgatgatgg cttctacttt gcccatcgct ttttcagccg taccttcggg gatttgtttc
     1501 gatatttgac ccagttcgcc cggcattttc gacatcaggt tttccaaacc gcccatattg
     1561 cgcatttgct ggatttgttc tttaaaatca ttgaggtcaa agcctttgcc tttttgcagc
     1621 tttttcgcca ttttggcggc ggcttcttca tcaatacctt tttgaacgtc ttcaatcagg
     1681 gtcaatacgt cgcccatgcc gaggatacgg ctggcaagac ggtcggggtg gaaaggttcg
     1741 aggccgttga ttttctcgcc gacgccgata aatttaatcg gtttgccggt tacatggcgt
     1801 acggacaatg ccgcaccgcc gcgcgagtcg ccgtccatct tggtcaatac gacgccgttc
     1861 agcggcaggg cttcattaaa tgcctgagcg gtatttaccg catcctgacc cagcatggca
     1921 tcgataacga acaaagtttc aaccgggttg accaccgcat gaagcgcttt gatttcgttc
     1981 atcatctctt catcgattgc caaacggccg gcggtatcga ccatcaatac atcgtaaaaa
     2041 tgttttttgg cgtaatcaac ggcggcagtt gcaatctcaa ccggtttttg gttggtatcg
     2101 gatgggaaaa aatctacgcc gacctgttcg gccaacaggc gcagctgttc aatcgcggca
     2161 gggcggtaaa cgtcggcgga taccaccaaa acttttttct tctgatcgtt tttcagcagg
     2221 cgggcgagtt tgccgacggt tgttgtctta ccggcaccct gcaaaccggc catcaaaatg
     2281 atggcaggcg gtgaaaccga taaatccagt gttttgtttt ccttgcccat cagttcgact
     2341 aaggcttggt tgaccacgcc gataaaggct tggtcaggcg tcaggctgcc cgccacttcc
     2401 tgaccgaggg ccttttcttt gacgttgttg acgaactctt tgacgacagg cagggcgaca
     2461 tccgcctcaa gcagggcgag tcggacttcg cgcaaggctt ctttaatatt gtcttcggtc
     2521 agtttggcct gcccccggat gtttttgaag acattgctga agcggccggt taaattgtct
     2581 aacatacttg gtccttggtc tgaagtaaga atagcttgcc ccatcagggg cattctttgt
     2641 taaaataaaa tcaaaataat ttgatgcggc ttgtgtgccg gacagcatat cggcaaatcc
     2701 gtcaaggctt gcccgaaacg gggattttac aattccaacg ttaaaagttc caatatttca
     2761 taagcggccg catacggcgc aacagtatag atagagaaag tccaccatgc cgacagtttt
     2821 catctttttg acggcggttt acgcaggatt gggtgcattt gcatggcact gccaacagca
     2881 ggggcgcggc cgggattacc cgtggaagac ggaattgccg gttttgggcg cggcattgac
     2941 cgtccacggc gcggcactgc tgatgccggt cattcaagac aaaatcatca ttatgggctt
     3001 cgggtattcc ggcagcctga ttgtttggat gatgctgttt atttattttg ccggcagctt
     3061 tttttatccg ctgcgcggag tgcagttgct gctgtatcct tgcgccgcac tgatgctgct
     3121 gtcaggtttg gttttcccgg gaaaattctc gggatatgaa attaccgact ttccctttat
     3181 gctgcatatc ggcacatcac tgcttgcata cgggctgttc ggcatcgcaa cattattgtc
     3241 cgttttgagc ttgctgctgc accggagcct gcaccgcaga aacttctcca agctcgcagg
     3301 attcctgccg tcgctgctca gtttggaaaa actcatgttc caggccatgt gggcaggttt
     3361 catcctgctg acctattccg tcgtcagcgg cacattcttt gccgaagccg tattcggcaa
     3421 acccatgaca tttacccata aaaccgtatt cggcatattg tcatggctga tttacggagg
     3481 actgctgctc aaacacagca tgaccgcatg gcgcggcaaa aaagccgccg tgtggaccat
     3541 catcggattt gtcagcctta tgattgccta tatgggcagc aagttcgtat tggaaatcat
     3601 tctgaaaaga taagaagagc caaca
//
LOCUS       DBJGGI010000109         3592 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.109, whole genome shotgun sequence.
ACCESSION   DBJGGI010000109 DBJGGI010000000
VERSION     DBJGGI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.109"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..2119
                     /locus_tag="ACSIGJ_002029"
     CDS             <1..2119
                     /locus_tag="ACSIGJ_002029"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HHQ1330817.1"
                     /translation="TAEARTLLFHTASYLKPEEQAELEKAVAYAFRAHDGQTRKSGDP
                     YITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVFGNTVAEMVDGLSK
                     LEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTLGSMRPDKRRRIAR
                     ETLEIYAQIANRIGLNNAYRELQDLSFQNLHPNRYETLKKAMDKSRKNRQDVVGKVLR
                     AFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGFRVIVNSIPACYAA
                     LGTLHTLYQPKPGRFKDYIAIPKSNGYQSLHTILVGPYGLPIEVQIRTREMDAVAEGG
                     IVGHWSYKSDSKTVDQAVLHTNQWLKNILDLQASSANAIEFLEHVKVDLFPNEIYILT
                     PKGKILTLPKGATPVDFAYAVHTDIGHKTVAARVNNVMMPLRTKLKTGDSVEIITSEH
                     AKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALSSLLPKDVLLSDGI
                     KEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFGSEVRLSSIKVDGQ
                     ESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPEQQLDADWENMNGQ
                     NYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTEGFVEFKFLLKVKN
                     LDQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(2186..3370)
                     /gene="coaBC"
                     /locus_tag="ACSIGJ_002030"
     CDS             complement(2186..3370)
                     /gene="coaBC"
                     /locus_tag="ACSIGJ_002030"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HHQ1330818.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTNLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACRENGM
                     GRMPEPAELLDLLPDLWTPKILRDKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEISLIHGQLQTTLPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDTLASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKTTNQIVIIDDDAELSFPETSKDEAAMRIVERLAVYL
                     NK"
BASE COUNT          938 a          897 c          889 g          868 t
ORIGIN      
        1 gaccgccgaa gcgcgtaccc tgcttttcca taccgcctcc tacctcaagc ccgaggaaca
       61 ggcggagctt gaaaaagctg tcgcctatgc gtttcgcgcc cacgacgggc aaacccgcaa
      121 aagcggggat ccctacatca cgcatccgat tgccgttgcg acgcagctcg ccctttggca
      181 tatggacata cagggtcttt gtgcaggcgt gatgcacgac gtattggaag atacgggcgt
      241 gacaaaaggg gaaatggcgg cggtgttcgg caatactgtt gccgagatgg tggacgggct
      301 gtccaaactc gaaaaactca aatttgaaga tcatgcggaa catcaggcgg agagtttccg
      361 caaactgatt ttggcgatga ccaaagatgt gcgcgtcatc gttgtcaaac ttgccgaccg
      421 cctgcacaat atgcggacgc tcggttcgat gcgcccggac aaacgccgcc ggattgcaag
      481 ggaaaccctt gaaatctacg cacagattgc caaccgcata ggtctgaata atgcgtatcg
      541 ggagcttcag gatttatcgt tccaaaacct gcatcccaac cgctacgaga ctttaaaaaa
      601 agcgatggac aagagccgga agaacaggca ggacgttgtc ggcaaagttt tgcgcgcgtt
      661 cggccagcgg ctggtaggcg cgaatataga ggccaaaatc aaaggcaggg aaaaaaacct
      721 gtacggcatc catcagaaaa tgatggcgaa aaagctgcgc tttgccgagg ttatggatat
      781 ttacggtttc cgcgtcattg tcaacagcat tcccgcctgt tatgccgcac tcggcacatt
      841 gcacaccctc tatcagccca agcccggacg gttcaaagac tatatcgcca ttccgaaaag
      901 caacggttat caaagtctgc atacgatttt ggtcggcccc tacggcttgc cgattgaagt
      961 tcagatacgc acgcgggaaa tggatgccgt ggcggaaggc ggtattgtcg gccattggag
     1021 ctataaatca gattccaaaa cggtcgatca ggcagtgctg cacaccaatc aatggttgaa
     1081 aaacatttta gacttgcagg caagcagcgc caatgccatt gagtttctcg aacacgtcaa
     1141 agtcgatttg tttccgaacg aaatctacat ccttactccg aaaggaaaaa tcctaacttt
     1201 gcccaaaggg gcaacgcctg tcgattttgc ttatgcggtg cataccgaca ttgggcataa
     1261 aacagttgcc gcgcgtgtca ataatgtgat gatgccgctg cgtacgaagc tcaaaaccgg
     1321 tgattctgtt gaaattatca catccgaaca cgccaaaccc aatcccgcgt ggttgaattt
     1381 cgccgtgtcc ggcagggcgc gcagtgccat acgccaatat attaaaaatc ttaaccggca
     1441 cgatgcggtc gttttgggag agagcctctt acaaaaagcc ttgtccagtt tgctgcccaa
     1501 agatgtcctg ctttcagacg gcatcaaaga aaaatacctt gccgatctca acgacaaaca
     1561 gacttcattt gaggaagtgc tgtacaacgt cggtatgggg cataccctgc ctgtttatgt
     1621 cgccatgcat atcgccgaat tggcagggga gcatttcggc agcgaggtca ggctcagttc
     1681 cattaaagtt gatggtcagg aaagcgggca tattcatttt gcagagtgct gccaccctgt
     1741 tcccggcgat tccatccgtt tgctgttggt taagggaaaa ggcatgatta tccataggga
     1801 tacctgcccg acgttgttga agtccgatcc cgaacagcag ctggatgcag actgggaaaa
     1861 tatgaacggg cagaactacc gtgtcgggct tcaagtccaa tcggaagaca gccacggcct
     1921 gttggcatta atggcgcaag cgatttccga ttccggtgcg gacatcgagt cggtcgaaac
     1981 accgtctaaa tcccagtcgg gaacggaagg ttttgtcgaa ttcaaattct tattgaaagt
     2041 caagaatttg gatcagttga atcagattat tcaaaatctg cacagcatcc catatatccg
     2101 caaagtcatc agaagttgaa gcaggtaaaa cgggttgcct gttaatcaaa aggcaacccg
     2161 ttttatggtt tatccgttca attgcttatt tgttcaaata tacggcaagc ctttcaacaa
     2221 tccgcattgc cgcttcatct ttacttgttt cggggaaaga caactccgca tcatcatcta
     2281 tgataacaat ttggttggtt gtcttgccca tcgcgattga aacatcattg gcaactatca
     2341 ccggtatttt tttcttaata cgtttttccc gcgcatatgc cattacattc tccgtttcag
     2401 cggcaaaacc gatgcagaac ggcgggttcg gcaatgaggc aatagaagcc aaagtatcgg
     2461 ggttctcatc caattcgatg gataacggtt tggcattttt atcttttttg aatttttgcg
     2521 tactcctgtt tttaacctta tagtctgaga cggcggcaac agaaataaaa gcatcttgtt
     2581 tttcaatcaa acgatgtact gcgcgatgca tatcttcggc actgacggct tgaaccgtat
     2641 cggatatgcc gaaaggcagc gtggtttgaa gctgtccgtg aatcaggctg atttccgcac
     2701 cggcggcacg gcacgcccgc gccaaagcta cgcccatttt tccgctggag gtatttgtga
     2761 tgcccctgac agggtcaatg gcttcaaatg tcgcacctgc ggtaatcaag attttcttgt
     2821 cccttaaaat tttcggtgtc cacaagtctg gaagcaaatc cagcaattcg gcaggttccg
     2881 gcatccttcc cataccattt tctctgcaag cctgttcgcc caagcccggc atatagacag
     2941 taatgccgtc tgaaaccagt tgtgcgatat tccgttggtt ggcaggattc agccacattt
     3001 ccacattcat cgcgggagcg atggcaagag ggcatttccg tgcggctgcc agattggtca
     3061 gtaggttatc tgccacgccg ttacagattt ttgccacggt attcatactt gccggcgcaa
     3121 tcagaaaaac atccgcattc cgggtcaggt tgatatgttc cataccgttt gaaccgttgc
     3181 cgccgtgcgt gtcggtcaga acgggattgc cgctcaaagc ctgaaaagtc agcggagaaa
     3241 caaattcagt tgccgagcgg ctcataacca ccgtaaccga atgcccctgt tttttcagca
     3301 gtcgcaccaa ctcgcaagac ttatacgccg caatactgcc cgttacacct aaaagaatat
     3361 gtttgcccat tttgacaatt tctaattacg aagaaaaaac agcagccatt ctataacaaa
     3421 gtacggatac attagttacc gtttggatac gaaatgccgt ctgaagcccc gagtttcagg
     3481 cggcatattc acaaaggcgc atcagccgga ggagaagagg gggttgttgg aggcggcgca
     3541 gcgtttggcg gagataaaaa accttatccg acagcgacat gacgaatttc cc
//
LOCUS       DBJGGI010000110         3564 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.110, whole genome shotgun sequence.
ACCESSION   DBJGGI010000110 DBJGGI010000000
VERSION     DBJGGI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.110"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(308..523)
                     /locus_tag="ACSIGJ_002031"
     CDS             complement(308..523)
                     /locus_tag="ACSIGJ_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330819.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPQKRESVFL"
     gene            complement(554..3322)
                     /gene="tdfH"
                     /locus_tag="ACSIGJ_002032"
     CDS             complement(554..3322)
                     /gene="tdfH"
                     /locus_tag="ACSIGJ_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc piracy receptor TdfH"
                     /protein_id="HHQ1330820.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAERVPKDKKVFTDARAVSTRQDIFKSGENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRGVAQNYRVGGGGQHIGNFGEEYLERRKQQYFVQEGGLKFNAGSGKWER
                     DLQRQYWKTKWYKKYEDPQELQKYIEGHDKSWRENLAPQYDITPIDPSGLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNAINYRFGGEYTGYYGSENEFKRAFGENSPAYKEHCDPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFAGYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDILGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVGST
                     GLAYTIRHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADKTLCNGKYGGTSKSVLTNFARGRTF
                     LMTMSYKF"
BASE COUNT          822 a          873 c          887 g          982 t
ORIGIN      
        1 agtaaataaa aaataacaac ataaatcaat ttattacggt tttattttta tttggtgttg
       61 acgggcaaca tatcatctgc gcggtaatga tgagatttta ggttgtggga tttatcggaa
      121 aaacagaaac ctctccgccg ccattctcac gaaagtggga atccggaaag ctaaagccac
      181 aggaatttat cggaaatggc tgaaatttat agtggattaa caaaaatcag gacaaggcga
      241 cgaagctgca gacagtacaa atagtacggc aaggcgaggc aacgccgtac ctgtttttgt
      301 taatccacta taaaaaaact gattcccgct tttgcgggaa tgacgactca taaattaccc
      361 gaaacaacca aaaataaccg aaatcaaaaa ccggcttccc tgcgtgggaa tgaaatggat
      421 ttttctttga gttgccgatg cgataaaacg gattggcata ggcttgcggc aggatgatgc
      481 gtttggggaa acaaatgccg tctgaagggc tttcagacgg cattgcagtt tttccacaaa
      541 acgcgggctg cctttaaaac ttgtagctca tcgtcatcaa gaaggtgcgt ccgcgtgcga
      601 aattggtcaa tacgcttttg cttgtgccgc cgtatttgcc gttgcacaac gttttatcag
      661 cattacacgt tacgtcttcg tccttgtctt tcgggtcgaa cgagctgtaa taacgctgcg
      721 ttgccgcatc attgcccgca tcgagcggat cgatataacg cctgtcgaac aggtttttga
      781 cttcggcgcg gaaaataagg tttttcttcg gctcgtaagc ggcgtaaaaa tcaaaaatca
      841 aaggctgtcg ggcaagggtt tcggtttgtt tgatggaacg cttgcccagt tgccggacat
      901 tgctggtatt tcccccgttg gtgccgtcga tatagcgttc ttcagccgtc gcgcggatgc
      961 tcttgccgaa atagcgcatc gcgccgccca aagtcagttt gttgcccaac cagcgcgtac
     1021 cgacttccaa acgtccgtaa tctcgcggca gggcggaaac cctgctcagc ccataacctt
     1081 gtttgagttg gtcttctttg gaggcattgt tgggcgattc gctcgcatcg ctgaaattgg
     1141 tcggttgcgt gcttttttga taggcgtaag aaaggttggt gaaaaaacgc ccataatcgt
     1201 aattcagctc cagctcaaaa ccgtgtttgt gcactttgtc tttgaaattg cggtgtcgga
     1261 tggtgtaggc aagcccggtg ctgccgaccc agctcggaat atccccgttc aaatcccacc
     1321 atttcccgta aacgttgtgg atgtagttgt caatgcggct gcggtagccg accagtttca
     1381 atcctaatat atcatcttgt tttaacaatc cttttttata ggtattgaag ccaaattgcc
     1441 aagtgtttgc gcgctctggt tttaaggcgg tgtgaacgcc ggagtcgccg atttgggaaa
     1501 aatacatttc ttggatgttg ggcatacggt gtgtgcgcga atagccggcg aacggcatga
     1561 aataatcgcc gaagtccgca ctaatgctga ccgaatggtt gttggcgcgc tttttgccgt
     1621 attttttcaa tacgggttca taaagcccgc agctcgggtc gcaatgttcc ttgtatgccg
     1681 gcgagttttc tccgaatgcc cgcttaaatt cgttttccga gccgtaatag cccgtatatt
     1741 cgccgccgaa acggtagttg attgcattgg tgctgtagtt taagcggtaa atgtcttttt
     1801 tgagcgcggc atcgaagtag aacgtgttga aatattgact gccggccggt tggacgatgg
     1861 ttgatttttg gggcagcagc cctttatcgc ccttaaaccg ccccaaatag gaataaagcc
     1921 cgttgtcctg atccggaccg tcgaaaaaca gccccaattc ttcaggaaag cggtttttgc
     1981 cgtattcgtt gtggaaataa ttgaagccca aagtggtttg caactcggtt tcgcggggca
     2041 gccggaaggt ggcggtgttg ttgaggtcga ggatttttgc gttgttgtag gtttcaaaat
     2101 cctttaaaag cccccagcct gtaaacttcg accctttcgg atatttctgc ctgcccgaat
     2161 tgtaggctgc ggtcagattg aggttggtat acgggttcaa agacaaaccg taattgaatt
     2221 gataattgcg gttgatgatt ttgcggctgc cgattttggt gtttaaatcg cgaaattgcg
     2281 ccgtgtattt attgaatacg ccgtcgtatt ccaatttaaa cagattacct gccgactgct
     2341 gcttcaggcc ggacggatcg atgggggtga tgtcgtattg cggcgccagg ttttcccgcc
     2401 agcttttgtc atgaccttcg atgtattttt gcagttcttg ggggtcttcg tattttttat
     2461 accactttgt tttccagtat tgcctttgca aatcccgttc ccattttccg ctgccggcat
     2521 tgaatttcaa accaccctct tgtacaaaat attgctgttt gcgccgttcc agatattctt
     2581 caccaaaatt tccgatgtgc tgcccgccgc cgcccacgcg gtaattttgc gccacgccgc
     2641 gcctgctgtg cccgtaaagc acaccgacag atgctccgct ttccagccat ttgcgcgcac
     2701 ctatcgccgc catcgcattg cctttggttg aattggtgcc ggtcagacct tttagcagca
     2761 ggccgtaggt attattgccc tgaacgacgt catccacgcc taaagtccgc agattcgccg
     2821 aaccggcaag gctgttgatg cctgccgagc cgctgaagct gcctttgacg acatccagtc
     2881 cggcaataaa attgctgtcg acagatgcac cgaattgaga tgaaccgcct gccctgcccg
     2941 catcggtaga agtcgaataa aaggtctgcg tgatgccgtc caccatcgta ttgacccgcc
     3001 cgaacccgct gtcgccgcga atattcaaag acacaatgcc cgagctttta tcttgctgtg
     3061 taaacgcacc gggtatgcta cgtacgatgt tgtcgaggtt ttcgccggat ttgaatatat
     3121 cctgacgggt cgatacggca cgcgcatcgg taaacacttt tttgtctttc ggtacgcgct
     3181 cagccttgac gtgcacgtct tccaaaacct gtatctgcgc ctcgctgccc gcgcgccctg
     3241 catcttctgc ataactatga tgatatagca taacacccat aagataaaaa caaatcggct
     3301 tcaaccggaa agaagatctc atattttcct caacaataaa caatcaatcc aacatgtcta
     3361 ccgttttcat cgaatccgcc ttttcgacca cccggcccta cgcaaccgaa ccgtcattcc
     3421 cacggaagtg ggaatctagg acgcggggtt tcagtcattt ccgatagatt cccgccgcgt
     3481 cgggggtccg gattcccgcc tgcgcgggaa tgacgggttt cgagattgcg gtgttgtcgg
     3541 gaatgacggt tcgggtattt tact
//
LOCUS       DBJGGI010000111         3424 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.111, whole genome shotgun sequence.
ACCESSION   DBJGGI010000111 DBJGGI010000000
VERSION     DBJGGI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.111"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            369..>3424
                     /gene="pilC"
                     /locus_tag="ACSIGJ_002033"
     CDS             369..>3424
                     /gene="pilC"
                     /locus_tag="ACSIGJ_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HHQ1330821.1"
                     /translation="MNKTLKRRVFRHTALYTAILMFSHTGGGGAMAQTHQYAIIMNER
                     KQPEVKWKDQYNQSALKDKSRERTFSHTSQKNSLGITSNFISFNNNDELVSQQSGTAV
                     FGTATYLPPYGKVSGFDTAELNKRGNAVNWIHTTRPGLAGYGYTGIRCGSTKDCPKLT
                     YKTRFSFDNPDLLKTRGGLDRHTEPSRENSPIYKLKDYPWLGVSFNLGGEGTAKDGRS
                     SSKLVSSFDENNSNSNQNLVYTTEDHRISLSNWQQETTAMAYYLNAKLHLLDKKGIKD
                     ITGKTVRLGVLKPSIDVKTQNTGLSGILNFWSKWDIKDNGQIPVKLGLPEVKAGRCIN
                     EPNPNKNTQAPSPALTAPALWFGSVQNGKVQMYSASVSTYPGSSSSRIFLQELKTRTD
                     PARPGRHSLAALDTQNIKSREPNFNSRQTVIRLPGGVYRIAPTRDRIVGLNGNDGKND
                     TFGIYKDRLVTPEVDEWAKVLLPWTVRYYGNDDIFKTFNQPNNKKQSDKKQYSQKYRI
                     RTKEDDNDKPRDLGDIVNSPITAVGGYLATSANDGMVHIFKKTGTDQRGYELKLSYIP
                     GTMERKDIEGNDSDLAKELRAFAEQGYVGDRYGVDGGFVLRRITDDQDKQKHFFMFGA
                     MGLGGRGAYALDLTKIDSNPVGVSMFDVQNDKNNNKNDSNRVKLGYTVGTPQIGKTQN
                     GKYAAFLASGYAAKDIVSSDNTTALYVYDLKNTLGTPIAKIEAPGGKGGLSSPTLVDK
                     DLDGTVDIAYAGDRGGNMYRFDLSSDKPSEWAVRTIFEGTKPITSAPAVSRLADKRVV
                     IFGTGSDLSEEDVVGKDQQYIYGIFDDDKGTGTVKVTVQNGTGGGLLEQTLTKENNTL
                     FLSNNKASGGSADKGWVVKLREGERVTVKPTVVLRTAFVTIRKYKDDGCGADTAILGI
                     NTADGGALTPRSARPIVPDHNSVAQYSGHKKTTDGKSVPIGCMWKNSKTVCPNGYVYD
                     KPVNVRYLDEKKTDDFPVTADGDAGGSGTFKEGKKPARNNRCFSGKG"
BASE COUNT          950 a          913 c          904 g          657 t
ORIGIN      
        1 attgccgaaa accgctttac catgatcagc gcgcaagggt agaataatgc ggtaacccta
       61 tacattgcat ccggccaaag gggcgtgtta cctttgcgaa catccccctt tggcagccgg
      121 gcgaaggggg ctttgcaacc ggcaatccgg cgggcgcggg atcgggcggt ttgcccaatc
      181 ccgccgtttg cggcgcgcct gccgccgacg gtatcccgcg aagcaagatt taagggataa
      241 aatatgttcc aacacgcagg gcggcacata aggcgccgcc ctgattcgga agggcttgca
      301 cccctcccgg acaaagcctg atcctgccgc cccgaagggc gggggggttt gaccgaaaag
      361 gaaatacgat gaataaaact ttaaaaaggc gggttttccg ccataccgcg ctttataccg
      421 ccatcttgat gttttcccat accggcgggg ggggggcgat ggcgcaaacc catcaatacg
      481 ctattatcat gaacgagcga aagcagcccg aggtaaagtg gaaggatcaa tataatcaat
      541 cagcattaaa ggacaaaagc agggagcgga catttagcca tacgagccag aaaaacagcc
      601 tcggcatcac aagcaatttt atctcattca acaataacga tgagcttgtt tctcaacaaa
      661 gcggtactgc cgtttttggc acagccacct acctgccgcc ctacggcaag gtttccggct
      721 ttgataccgc cgagctgaac aagcgcggca atgccgtcaa ttggattcat accacccggc
      781 ccgggctggc aggctacggc tacaccggta tccgttgcgg aagtaccaaa gactgtccca
      841 aacttaccta taaaacccga ttttccttcg ataatcccga cttgctaaaa acaagaggcg
      901 ggctggatag gcacacagag ccaagccgcg aaaattcgcc catttacaaa ttgaaggatt
      961 atccatggtt gggtgtgtct ttcaatttgg gcggcgaggg taccgccaaa gatggcagat
     1021 catccagcaa attggtatct tcttttgatg aaaacaatag taatagtaat caaaacctcg
     1081 tctatacgac agaagaccac cgtatttccc ttagcaactg gcagcaagaa acgaccgcca
     1141 tggcctatta tctgaacgcc aagctgcacc tgctggacaa aaaagggatt aaagatatca
     1201 ccggcaaaac agtgcggttg ggtgtcttga agccgagcat cgatgtgaag acacaaaata
     1261 cggggcttag cggcattcta aatttttggt ctaagtggga cattaaagat aacgggcaga
     1321 ttccggtcaa gctcggcctg ccggaagtca aagcaggccg ctgcatcaac gaaccgaacc
     1381 ccaataagaa tacccaagcc ccttcgccgg cactgaccgc ccccgcgctg tggttcggct
     1441 ctgtgcaaaa tggtaaggtg cagatgtatt ccgcttcggt ttccacctac cccggcagtt
     1501 cgagcagccg catcttcctc caagagctga aaactcgaac cgaccccgcc cggcccggcc
     1561 ggcattccct cgccgctttg gatacgcaga acatcaaaag ccgcgagccg aatttcaact
     1621 caaggcagac cgtgatccga ttgccgggcg gcgtgtaccg gatcgccccg actcgcgaca
     1681 ggatcgtggg tttgaatggc aatgacggca aaaacgacac tttcggcatc tacaaggaca
     1741 ggctcgtcac acctgaggtc gacgagtggg caaaagtgct gctgccttgg acggtccggt
     1801 attacggtaa tgacgatata tttaaaacat tcaaccaacc aaacaacaaa aaacaaagcg
     1861 acaaaaaaca atacagccaa aaataccgca tccgcacaaa agaagatgac aatgacaaac
     1921 cccgcgattt gggcgacatc gtcaacagcc cgataacggc ggtcggcggg tatctggcaa
     1981 cttctgccaa cgacgggatg gtgcatatct tcaaaaaaac cggcacagac caacgaggct
     2041 acgaactgaa gctcagctac atccccggta cgatggagcg taaggatatt gaaggcaatg
     2101 actccgacct cgccaaagag ctgcgcgcct ttgccgaaca aggctatgtg ggcgaccgct
     2161 acggcgtgga cggcggcttt gtcttgcgcc gcattacaga tgaccaagac aagcaaaaac
     2221 atttctttat gtttggtgcg atgggcctgg gcggcagagg cgcatacgcc ttggatttga
     2281 ccaaaatcga cagcaacccg gtcggcgttt ccatgtttga tgtccaaaac gacaaaaata
     2341 acaataagaa tgacagtaat cgcgtgaaat taggctacac cgtcggtacg ccgcaaatcg
     2401 gcaaaaccca aaacggcaaa tacgccgcct tcctcgcctc cggttatgcg gctaaagata
     2461 ttgtcagcag cgataataca accgcgctgt atgtgtatga tttgaaaaac accttaggta
     2521 cgccgattgc aaaaatcgaa gcacccggcg gcaaaggcgg gctttcgtcc cccacgctgg
     2581 tggataaaga tttggacggc acggtcgata tcgcctatgc cggcgatcgg ggcgggaata
     2641 tgtaccgctt tgatttgagc agcgacaagc cgtccgagtg ggctgtacgc actattttcg
     2701 aaggcacaaa accgattact tccgcgcccg ccgtttcccg actggcagac aaacgcgtgg
     2761 tcatcttcgg tacgggcagc gatttgagtg aagaggatgt agtcggtaag gatcaacaat
     2821 atatttacgg tatctttgac gacgataagg ggacggggac ggttaaggta acggtacaaa
     2881 acggcacggg aggcgggctg ctcgagcaaa cgcttacgaa ggaaaataac accttgttcc
     2941 tgagcaataa taaggcatcc ggcggatcgg ccgataaagg ctgggtagtg aaattgaggg
     3001 aaggagaacg cgttaccgtc aaaccgaccg tggtattgcg taccgccttc gtaaccatcc
     3061 gcaagtataa agacgacggc tgcggcgcgg ataccgccat tttgggcatc aataccgccg
     3121 acggcggcgc attgactccg agaagcgcgc gcccgattgt gccggatcac aattcggttg
     3181 cgcaatattc cggccataag aaaaccaccg acggcaagtc cgtccccata ggctgtatgt
     3241 ggaaaaacag caaaaccgtc tgcccgaacg gatatgttta cgacaaaccg gttaatgtgc
     3301 gctacctgga cgaaaagaaa acagacgatt tccccgtcac ggcagacggt gatgcaggcg
     3361 gcagcggaac attcaaagag ggtaaaaaac ccgcccgcaa taaccggtgc ttctccggaa
     3421 aagg
//
LOCUS       DBJGGI010000112         3242 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.112, whole genome shotgun sequence.
ACCESSION   DBJGGI010000112 DBJGGI010000000
VERSION     DBJGGI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.112"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..690
                     /gene="mafB"
                     /locus_tag="ACSIGJ_002034"
     CDS             <1..690
                     /gene="mafB"
                     /locus_tag="ACSIGJ_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HHQ1330822.1"
                     /translation="EASGETGYKPVTAQYGNPYRETVYIEISDGIGNNQTLISNRIHP
                     FYSDGKWIKAEDLKAGSRLLSESGRTQTVRNIIVKPKPLKAYNLTVADWHTYFVKGDK
                     AETEGVWVHNDCPTKLKPTERYNRQTHYGGSQTDGARAQAARQAGEGKPCPTCGRIQI
                     FGTKTAPSPQHEPPLVKHYYEHGGHSMSNADRAKHARESIKGTQCLTCQRKEGAMMSR
                     YSREQAKKHGL"
     gene            680..1228
                     /locus_tag="ACSIGJ_002035"
     CDS             680..1228
                     /locus_tag="ACSIGJ_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HHQ1330823.1"
                     /translation="MDYNQTVLSHLQKFWKHHDIKGFTWTLGRIVEELPDFQVFQVIP
                     NHEDEPWVYVSSGIGQFLGQEFFIISPFETPEHIETLAMLASASMHYPDQFQLGKTVN
                     IGRPWVEQSSFRHFLISLPYPYGQELEYMDNVRFFWLLPITQTERLFLNTHSVEELET
                     KFDEAGIDYLDINRASTVWQAG"
     gene            1525..1878
                     /locus_tag="ACSIGJ_002036"
     CDS             1525..1878
                     /locus_tag="ACSIGJ_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein Imm33 domain-containing
                     protein"
                     /protein_id="HHQ1330824.1"
                     /translation="MGIKNEQIIICKKYNTEIYPVSDVSKIGVAENVKQTGLYPINGL
                     RHRPKGDTNGWYIWAGENFSYDKNFFLPLHTFHLQIWRPEIIPFLTLPPGYRFLIGEN
                     GYEDVWFDELLLNDN"
     gene            1868..2191
                     /locus_tag="ACSIGJ_002037"
     CDS             1868..2191
                     /locus_tag="ACSIGJ_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HHQ1330825.1"
                     /translation="MIIDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     QLISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            2250..2477
                     /locus_tag="ACSIGJ_002038"
     CDS             2250..2477
                     /locus_tag="ACSIGJ_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330826.1"
                     /translation="MDIKFNTLGVILNGVNPEEKFIKIIDGQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            2604..3147
                     /locus_tag="ACSIGJ_002039"
                     /pseudo
     CDS             2604..3147
                     /locus_tag="ACSIGJ_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
BASE COUNT         1058 a          576 c          681 g          927 t
ORIGIN      
        1 gaggcaagcg gggagacggg atacaaaccc gttaccgccc aatacggcaa tccgtatcgg
       61 gaaaccgttt acattgaaat ttcagacggc atcggcaaca accaaaccct gatttccaac
      121 cgcatccatc cgttttattc ggacggcaaa tggattaaag cggaagattt aaaggcggga
      181 agcagactgt tatccgaaag cggcagaacc caaaccgtcc gcaacatcat tgtcaaacca
      241 aaaccgctca aagcctacaa tctgaccgtt gccgattggc atacctactt tgtcaaaggc
      301 gataaggcgg aaacggaagg ggtttgggtt cataatgatt gtccaaccaa actaaaaccg
      361 acagaacgtt ataatcgtca aacgcattat ggaggttctc aaacagatgg agcaagggct
      421 caggcagcaa gacaggcagg agaaggtaaa ccttgtccaa cttgtggtag aattcagata
      481 tttggtacga aaaccgcacc aagtccgcaa catgaacccc cattagtaaa acattactat
      541 gaacatggtg gtcactctat gagcaatgct gatcgagcga aacatgccag ggaaagcata
      601 aaaggaactc aatgtttaac atgtcaaagg aaagagggtg cgatgatgtc aagatattcg
      661 agagaacagg ctaaaaagca tggactataa ccaaactgtt ttatctcact tgcaaaaatt
      721 ttggaaacat catgatatta aaggatttac ttggacttta ggaagaattg ttgaagaact
      781 tcccgatttc caagttttcc aagtgattcc taatcatgaa gatgagcctt gggtttatgt
      841 cagctctggg ataggtcaat ttttagggca ggagtttttt atcatcagcc cgtttgaaac
      901 acctgaacat attgagacct tggcaatgct ggcatctgca agtatgcatt atcctgatca
      961 atttcaatta gggaaaaccg tcaatatcgg tagaccgtgg gtcgagcaat catccttccg
     1021 gcattttctg atttctctgc cttatcctta tgggcaagag ttggaatata tggataatgt
     1081 ccgatttttc tggttattgc caattaccca gacagaaaga cttttcttga atactcattc
     1141 ggtagaagaa ttggaaacga aatttgatga agcgggtatt gattatttag atataaatcg
     1201 tgcgagtact gtctggcagg caggataaaa attcggcaat caaaatgccg tctgaaacaa
     1261 gtttggactt tcagacggta ttctgatgaa tggaacagga tactgggaac tgagttcttc
     1321 aaaaatccta tctccgtccc ttatacgtcg gtgctttagt caaaacggca gacggatgaa
     1381 aaattattgc ccgtatcctt gccaaagatg aagcaagatg aagccaggta attgaatcgg
     1441 tacaagcgca atgtccgact tgttctgcaa aacagggagg gagattaagc caatattcaa
     1501 aagaacagaa aaagaaactg agtaatggga ataaaaaatg aacaaataat aatttgtaaa
     1561 aaatataata cagaaatata tcctgtttcc gatgtttcaa aaatcggtgt tgctgaaaat
     1621 gtcaaacaga caggcctgta tccgattaac gggttaaggc atcgtcccaa aggtgatact
     1681 aacggttggt atatctgggc cggtgaaaac ttctcctatg ataaaaattt tttcctgccc
     1741 ttgcatacgt tccatttgca aatatggcga cccgaaatca tccccttttt gacacttccg
     1801 cccggttacc gttttttaat tggggaaaac ggttatgaag acgtatggtt tgatgaattg
     1861 ctattaaatg ataattgacg aaaaatctac ttcagatgta ctgaaacaat atcaagaact
     1921 agggtttgat ttgacaaaac ctatgataat agagtttttt ataggaggtt ctcaaaaaaa
     1981 tctgcaatca atagaaagcc aactcatttc ttatcggcaa gattttcaaa tcagtattga
     2041 acaagatgaa ttcggagaaa tgtggacttg ctattgttcc gtcaatatcg tcccttccct
     2101 tgaaaacatt ttagctattg aaactacgtt atttggtatt gcacaaaaaa atgattgtaa
     2161 ttatgaaggt tttggttcgt atgggaacta acaaaaagtg taggttgggt tgaaaaaacc
     2221 atattcccat taaagattag ggtgtgtcta tggacataaa attcaatact ttgggggtta
     2281 tcctgaatgg ggtcaatcct gaagaaaaat tcataaaaat tattgatggc caggaaaata
     2341 ccggtggatt tctgatactg ttatcttcaa atgacaaatt ctcatcattt gacagttacg
     2401 atgactgggt cgaaaactta gaaatattaa aagaatacct gcaagaatct cattggataa
     2461 taaagtgggt cggatagtcg gcaaacgtag tccgtttgaa acgcttttac ttttcagacg
     2521 gactttctac caatgggaac aaggcgtttt gtctcaatcc ggtttatgat tattcgatac
     2581 agtgatggga agctaagaaa aatatgatta atcatattga aaaatatata ggcactattt
     2641 ctcatggctc gaaaagcgat tcgggaagtc agtacaaatt aaatatcgca gctattccct
     2701 cttcccctaa cagggattta aaaacttata ttactttagg gttaagcaaa catgatctga
     2761 attataaaag cagatttgaa atactttttg tatgttcttt aaaatatgat gaaaatcaga
     2821 ttttcccatt tttaagatgg ttagcagaaa ccatcattga gaataaaaaa aatccttctt
     2881 cgggggcagg ttgtttattt gcctagaagc attgttaatt caacaaaaat ggacgcactg
     2941 tatgtttccg ccccatttta tttcgacgat gatttccaag tctgttatgg cgaacactac
     3001 aatattgttt tccctttgct tgtccccttg tataaacagg aagccgaatt ggtggaaaaa
     3061 aagggttgga atgcttttga gcagttcttg ttggataatg aagttggcaa cctttcggat
     3121 atgaatagga agccgtttgt ttggtaaggg ctttcttaaa tttgattaaa ggtcgtctga
     3181 aaccgtgttt aattttcaga cggcatttcc ttcatttgaa acaggatatt gaaaactaag
     3241 tt
//
LOCUS       DBJGGI010000113         3078 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.113, whole genome shotgun sequence.
ACCESSION   DBJGGI010000113 DBJGGI010000000
VERSION     DBJGGI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.113"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            3..335
                     /locus_tag="ACSIGJ_002040"
     CDS             3..335
                     /locus_tag="ACSIGJ_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330827.1"
                     /translation="MKYYGTAAYGSPDWGMERYYAREDMRQALDGWEAENRRLHESGL
                     IEIAKKSAREFVRDADGEPYAQEDWETYLTEDASRIDKDTEAAMNYAIDEREWFALAE
                     NIGRLANS"
     gene            489..764
                     /locus_tag="ACSIGJ_002041"
     CDS             489..764
                     /locus_tag="ACSIGJ_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO1622 family putative holin"
                     /protein_id="HHQ1330828.1"
                     /translation="MKTGNIAFKFAVLAAVLAAGYAFGFAKGGGSRTAKGKPAGIAAM
                     RMALAQKQAEVDELSAEIWLEERHLNAGEEAGCRRVHGDAEVPEGKE"
     gene            761..922
                     /locus_tag="ACSIGJ_002042"
     CDS             761..922
                     /locus_tag="ACSIGJ_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330829.1"
                     /translation="MSFHPETAYTGGGETEPYGPSPEEIKYRQSPETAETRRMTEKQA
                     EGHIKSIIR"
     gene            991..1677
                     /locus_tag="ACSIGJ_002043"
     CDS             991..1677
                     /locus_tag="ACSIGJ_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replication initiation protein, NGO0469
                     family"
                     /protein_id="HHQ1330830.1"
                     /translation="MSLILSVKDESNFKPCPAGSHHATCIRIIDLGTQLVEYQNEQKR
                     QHKILVQWEIDPEGDPEMLMPDGRPYLISRRYTASLHSKSQLATDLKSWRGRDFTPEE
                     RDNFDLRNILGKPCLLSIAHQESSDGKTTYANISAISNKMKSYTPKQPDNAVFAFDLS
                     DPDWANYGLLNEKLREQIAKSPEYAEAVNGRQPPAPPQKQAQATEGQTEYPQGNAAPA
                     EDIEDDIPFN"
     gene            1818..2000
                     /locus_tag="ACSIGJ_002044"
     CDS             1818..2000
                     /locus_tag="ACSIGJ_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330831.1"
                     /translation="MANIDLTQWDGKTIGAAANPEQGYINITIGSDDLFINIEQAYAI
                     HAALGEAVAEYEGGAQ"
     gene            1997..2488
                     /locus_tag="ACSIGJ_002045"
     CDS             1997..2488
                     /locus_tag="ACSIGJ_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HHQ1330832.1"
                     /translation="MTALTLYRCAADVQAALDYYFDSETEREDTLEAVIGQFEVKAQS
                     VIAYIKNQEITEKMLEGHIRQMTGKLKAAKARNQSLKDYLARNMQAAGITEIKADDGT
                     FKASFRKSEAVVILDEAQIPAEFMREAVKTEPDKTAIRKAIESGRQVAGAKIEGRKNL
                     QIR"
     gene            2540..2755
                     /locus_tag="ACSIGJ_002046"
     CDS             2540..2755
                     /locus_tag="ACSIGJ_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330833.1"
                     /translation="MSYLEDVKNALRVIDNLCKEALKEPESLEGYIDEIRDKADEADT
                     SLEFLKDVINYGISDLKNVIEVFEDCV"
     gene            2866..>3078
                     /locus_tag="ACSIGJ_002047"
     CDS             2866..>3078
                     /locus_tag="ACSIGJ_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330834.1"
                     /translation="MQTVATKPTAKQMLAAKRAAKESTRQERAVKRAGTVKNVDRNRL
                     SARSKAQKENIARMLSGAKVSEDEALT"
BASE COUNT          937 a          769 c          874 g          498 t
ORIGIN      
        1 cggtgaaata ttacggcacg gcggcttacg gcagccccga ttgggggatg gaaagatatt
       61 acgcgcggga ggatatgcgg caggctttgg acggttggga ggcggaaaac cgccgcctgc
      121 acgaatccgg gttgattgag attgccaaaa aatcggcgcg ggagtttgtc cgcgatgcgg
      181 acggcgagcc ttacgcccaa gaagattggg aaacgtacct tacggaagat gcttcccgta
      241 tcgataagga taccgaagcc gcgatgaact acgccataga cgagcgggaa tggttcgcgc
      301 tggcggagaa catcggcagg ctggcaaatt catagcgggc ataggaatgc cgcgccgtca
      361 gccggggagc gggcgcggcg gcggtttttg tagattttag ccgcaaataa ccccgacggc
      421 gcacggtccg taccatcctt agcggacgcg gtgcgcaatt tgaaaccttt gaaaccaagg
      481 agatgaaaat gaagacaggg aatattgcgt ttaagttcgc ggtattggcg gcggtattgg
      541 cggcgggcta tgccttcgga ttcgccaagg gaggcggaag ccgtacggca aagggaaaac
      601 ccgccggtat tgcggcaatg cgcatggcgt tggctcaaaa gcaggcggaa gttgacgaat
      661 tgagcgccga gatctggctg gaagagcggc atctgaatgc gggggaagaa gccggttgcc
      721 ggcgggtgca cggcgatgcg gaagtgccgg agggtaaaga atgagcttcc atcccgaaac
      781 cgcttacacc ggcggcggag aaacagagcc gtacggacca agccccgaag aaatcaaata
      841 ccggcaaagc ccggaaaccg ccgaaacacg gcggatgacc gaaaaacagg cagaaggcca
      901 cattaaaagc attatcagat aacgccgccg cgcaaggcat aaaacagcgc gagaccgata
      961 gacgaaacga cctttaacag gagaccgaaa atgtcattga ttttaagtgt gaaagacgaa
     1021 agcaatttca aaccatgccc ggcaggcagc catcacgcca cctgcatccg catcatcgat
     1081 ttgggtacgc agctcgtcga gtaccaaaac gaacaaaagc ggcagcacaa gattttagtg
     1141 cagtgggaaa tcgaccctga aggcgatccg gaaatgctga tgccggacgg caggccctac
     1201 ctcatcagcc gccgatacac cgccagcctg cacagcaaaa gccaactggc aacagacctc
     1261 aaaagctggc gcggaaggga ctttacaccg gaagaacgcg acaacttcga tttgcgcaat
     1321 attttgggca agccctgcct gttgagtatc gcccaccaag aaagcagcga cggcaaaacc
     1381 acctatgcca acatttccgc catcagcaac aagatgaaga gctacacccc gaaacagccg
     1441 gacaacgccg tttttgcctt cgacctgtca gaccccgatt gggcgaatta cggcctcctg
     1501 aacgagaaac tgagggagca gattgccaaa agcccggaat atgccgaagc cgtaaacggc
     1561 cgccaaccgc cggcgccgcc gcagaaacag gcccaagcga cggaagggca gacggaatat
     1621 ccgcaaggca atgccgcgcc tgccgaagac atcgaggacg acatcccttt taattaagcc
     1681 cgccgtcagg gcgtaaaacc ggacggaaaa ccgcaaacaa acgccgcgcc ctgcgggcgg
     1741 gccccgcact ctgaagcagt agtcgatttt tagtttgcaa ctgcttcgga cggcaacaga
     1801 gaaaggaaac aaaaaaaatg gcaaacatcg acctgaccca atgggacggg aaaaccattg
     1861 gcgccgccgc caatcccgaa cagggataca tcaacatcac catcggcagc gacgacctat
     1921 tcatcaacat cgaacaggca tacgccatac acgccgcgct tggcgaagcg gttgccgaat
     1981 atgagggagg ggcacaatga ccgccctcac actctaccgg tgcgcggcag acgtacaggc
     2041 ggcgctggat tactacttcg acagcgaaac cgagcgcgaa gacacgctgg aagccgttat
     2101 cgggcagttc gaggtcaaag cgcaatccgt tatcgcttat attaaaaacc aagaaatcac
     2161 ggaaaaaatg cttgaagggc acatcaggca gatgaccggg aagctcaagg cggcaaaagc
     2221 gcggaatcaa agcctgaaag actacttggc gcgcaatatg caggcggcgg gcattaccga
     2281 aatcaaagcg gacgacggca cttttaaagc ctcgttccgc aaatccgaag ccgtcgtgat
     2341 cttagacgaa gcacaaatcc ccgccgaatt tatgcgtgag gccgtcaaaa ccgaaccgga
     2401 caaaaccgcc atcagaaaag cgattgaaag cggtcggcaa gtagcaggcg cgaagattga
     2461 agggcggaag aatttgcaga ttagataaac cgtagaagat gttgacgaca gcatcccatt
     2521 ttgagttaag gagcaaaaaa tgagctattt ggaagatgta aaaaacgcat taagggtaat
     2581 agataactta tgcaaagaag cattaaaaga gcctgaatcg ttagagggtt atatagacga
     2641 aattagggat aaagcagacg aagcggatac ctctttggaa tttctaaagg atgtaataaa
     2701 ttatggtatt agcgatttaa aaaatgtaat tgaggtgttt gaagattgcg tttgatatta
     2761 atggaacaag acatgaacta tcaatttaaa ttcggcgacc tcgtgagaga tacagcttta
     2821 tgattgggca ttgagagaac ggcatcaaaa aatcgagaaa acaaaatgca aacagtagca
     2881 acaaaaccga cggcaaaaca gatgcttgcc gccaaacgcg cggcgaagga atcaacgcgg
     2941 caggaacgcg ccgtcaaacg cgcgggaaca gtaaaaaacg ttgaccggaa ccggctgtcc
     3001 gcccggtcaa aagcgcaaaa agaaaacatc gcccggatgt tgtcgggcgc aaaggtatcg
     3061 gaagacgaag ccctgacg
//
LOCUS       DBJGGI010000114         3069 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.114, whole genome shotgun sequence.
ACCESSION   DBJGGI010000114 DBJGGI010000000
VERSION     DBJGGI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.114"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..2975
                     /gene="pilC"
                     /locus_tag="ACSIGJ_002048"
     CDS             <1..2975
                     /gene="pilC"
                     /locus_tag="ACSIGJ_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HHQ1330835.1"
                     /translation="RNQPEVKSNVPSSIKDKDRKREYTHHRYPRGGGSVSFDNTDTLV
                     SQQSGTAVFGTATYLPPYGKVSGFDTDRLKERANAAGWIRTTRPGLAGYSYADVVCRD
                     ITGCPKLVYETKFTFGQQGLQRKAGNKLDIYEDKSRENSPIYKLKDHPWLGVSFNLGS
                     ENTVKNSKSFNKLISFFSEDNNNQNIVSTTRDHPISLGDSKREHTAVAYYLNAKLHLL
                     DKKEIKDIAPGKTVDLGTLRPRVETIGRGLLDFWATWKIEDKGNITVRLGLPEVKAGR
                     CTNAAHPNPKVKPLSPALTAPALWFGPAQNGKVQMYSASVSTYPGSSSSKIFLQNLSR
                     NDDKNKPGRYSLKPLSENEIKSKEPSFMGRQTIIRLDDGVHLIRLSRSKDEVAAFVNL
                     NGNNTGKNDTFGIVKEANVNLDADEWKKVLLPWTVRGPGNDDKFKSINREPGKYSQRY
                     RIRENGNRDLGDIVNSPIVAVGGYLATAANDGMVHIFKKNGGSDERSYNLKLSYIPGT
                     MPRQYFDNDTSALKDSTLAQELRAFAEKGYVGDRYGVDGGFVLRQVELSGQKHVFMFG
                     AMGLGGRGAYALDLSKINGNYPAAAPLFDVKNGDNNGKNRVKVELGYTVGTPQIGKIR
                     NGKYAAFLASGYAAKEIGGPTNKTALYVYDLKNTLGTPIAKIEVKDGKGGLSSPTLVD
                     KDLDGTVDIAYAGDRGGNMYRFDLSDSDSSKWSVSTIFQGTKPITSAPAVSRLADKRV
                     VIFGTGSDLSEEDVVGTNPQYIYGIFDDDKGTVKVTVQNGTGGGLLEQVLKEENKTLF
                     LNKGSDGSGSKGWVVKLKEGQRVTVKPTVVLRTAFVTIRKYKDDGCGADTAILGINTA
                     DGGALTPRSARPIVPEANTAVAQYSGHKTTSKGKSIPIGCMEKGGKTVCPNGYVYDKP
                     VNVRYLDETETDGFSTTADGDAGGSGIDPADRRPGKNNRCFSKKGVRTLLMNDLDSLD
                     ITGPMCGIKRLSWREVFF"
BASE COUNT          841 a          795 c          811 g          622 t
ORIGIN      
        1 ggcgaaacca gcccgaggta aagtcgaatg tgccatcttc aataaaggac aaagacagga
       61 agcgcgaata tactcatcat aggtacccac gaggaggagg ctctgtctca ttcgacaata
      121 ccgataccct tgtttctcaa caaagcggta ctgccgtttt tggcacagcc acctacctgc
      181 cgccctacgg caaggtttcc ggttttgata ccgataggct gaaagagcgc gccaatgccg
      241 ccggttggat tcgtaccacc cggcccgggc tggcaggcta cagctacgcc gatgtcgtat
      301 gcagagacat cacaggctgt cccaaacttg tctatgagac caaatttacc ttcggtcaac
      361 aagggttgca aagaaaggca ggcaacaagc tggatatata cgaagacaaa agccgcgaaa
      421 attcgcccat ttacaaattg aaggatcatc cctggttggg cgtgtctttc aatttgggca
      481 gcgagaatac cgtcaaaaat agcaaatcat tcaacaaatt gatatctttt tttagtgaag
      541 acaataataa tcaaaacatc gtctctacga cacgagacca ccctatttcc cttggcgact
      601 cgaagcgcga acataccgcc gtggcctatt atctgaacgc caaactgcac ctgctggata
      661 aaaaagagat taaagatatc gcgccaggca aaacagtgga tttgggcacc ttgagaccgc
      721 gcgtcgagac gataggacga ggcttgctag atttttgggc tacgtggaag attgaagata
      781 aagggaacat tacagtccgc ctcggcctgc cggaagtcaa agccgggcgc tgcaccaacg
      841 ccgcccaccc caatcccaag gtcaaacccc tttcgccggc actgaccgcc cccgcgctgt
      901 ggttcggccc tgcgcaaaat ggcaaggtgc agatgtattc cgcttcggtt tctacctacc
      961 ccggcagctc gagcagcaaa attttcctgc aaaacctttc ccgcaatgat gacaaaaaca
     1021 aaccgggccg ctattccctc aaacccttga gtgagaatga gattaaaagt aaagagccga
     1081 gtttcatggg gcggcaaaca atcatccgat tggatgacgg cgtacatttg atcagactga
     1141 gtagaagcaa ggatgaggtc gccgcttttg tcaatttaaa tggaaacaac accggcaaaa
     1201 acgacacttt cggcattgtt aaggaagcga acgtcaatct tgacgccgac gagtggaaaa
     1261 aagtgctgct gccttggacg gttcggggtc ccggtaatga tgataaattt aaatcaatta
     1321 accgagaacc aggcaaatac agccaaagat accgcatccg cgaaaacggc aatcgcgatt
     1381 tgggcgacat cgtcaacagc ccgattgtcg cggtcggcgg gtatttggca accgccgcga
     1441 acgacgggat ggtgcatatc ttcaaaaaaa acggcggcag tgatgaacgc agctacaatc
     1501 tgaagctcag ctacatcccc ggcacgatgc cgcgccaata ttttgataac gacacttccg
     1561 ctctcaaaga ctccaccctc gcccaagagc tgcgcgcctt tgccgaaaaa ggctatgtgg
     1621 gcgaccgcta cggcgtggac ggcggctttg tcttgcgcca agtcgaactg agcgggcaaa
     1681 aacacgtgtt tatgttcggc gcgatgggtt tgggcggcag aggcgcgtat gccttggatt
     1741 taagcaaaat caacggaaat tatccggccg ccgctcccct gtttgatgtc aaaaatggcg
     1801 ataataacgg caaaaatcgc gtgaaagtgg aattaggcta caccgtcggc acgccgcaaa
     1861 tcggcaaaat ccgcaacggc aaatacgccg ccttcctcgc ctccggttat gcggctaaag
     1921 aaattggcgg cccaacaaat aaaaccgcgc tgtatgtata tgatttgaaa aacaccttag
     1981 gtacgccgat tgcaaaaatc gaagtgaagg acggcaaggg cgggctttcg tcccccacgc
     2041 tggtggataa agatttggac ggcacggtcg atatcgccta tgccggcgac cggggcggca
     2101 atatgtaccg ctttgatttg agcgattccg attctagtaa atggtctgta agcactattt
     2161 tccaaggcac aaaaccgatt acctccgcgc ccgccgtttc ccgactggca gacaaacgcg
     2221 tggtcatctt cggcacgggc agcgatttga gtgaagagga tgtagtcggt acgaatccac
     2281 aatatattta cggtatcttt gacgacgata aggggacggt taaggtaacg gtacaaaacg
     2341 gcacgggagg cgggttgctc gagcaagtgc ttaaagagga aaataaaaca ttattcctga
     2401 acaagggatc cgacggatcg ggcagtaagg ggtgggtagt gaagctgaag gaaggacaac
     2461 gcgttaccgt caaaccgacc gtggtattgc gtaccgcctt cgtaaccatc cgcaagtata
     2521 aagacgacgg ctgcggcgcg gataccgcca ttttgggcat caataccgcc gacggcggcg
     2581 cattgactcc gagaagcgcg cgcccgattg tgccggaagc caatacggct gtcgcacaat
     2641 attccggcca taagacaacc tccaaaggca aatccatccc tataggttgt atggaaaaag
     2701 gcggtaaaac cgtctgcccg aacggatatg tttacgacaa accggttaat gtgcgctacc
     2761 tggatgaaac ggaaacagac ggattttcaa cgacggcgga cggcgatgcg ggcggcagcg
     2821 gtatagaccc cgccgacagg cgtcccggca aaaacaaccg ctgcttctcc aaaaaagggg
     2881 tgcgcaccct gctgatgaac gatttggaca gcttggatat taccggcccg atgtgcggta
     2941 tcaaacgctt aagctggcgc gaagtcttct tctgacccgc ccgcgcggcc ggtttttccg
     3001 caaatgccgt ccgaaaggtc ttcagacggc atttttttgc gtttttcggg agggggcggc
     3061 aaatgaaac
//
LOCUS       DBJGGI010000115         2958 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.115, whole genome shotgun sequence.
ACCESSION   DBJGGI010000115 DBJGGI010000000
VERSION     DBJGGI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.115"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            553..1350
                     /gene="truA"
                     /locus_tag="ACSIGJ_002049"
     CDS             553..1350
                     /gene="truA"
                     /locus_tag="ACSIGJ_002049"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HHQ1330836.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQAGGVPTVQAALETALARI
                     AGESVATTVAGRTDTGVHAAAQVVHFDTTAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PGFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRRAAALLVGEQDFS
                     SFRAAGCQAKSPVKTIYRAGLTQSAGLIRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPGAYGIVRPQIPEWL"
     gene            1867..2913
                     /gene="porB"
                     /locus_tag="ACSIGJ_002050"
     CDS             1867..2913
                     /gene="porB"
                     /locus_tag="ACSIGJ_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB.IB"
                     /protein_id="HHQ1330837.1"
                     /translation="MKKSLIALTLAALPVAATADVTLYGVIKAGVQTYRSVEHREGKV
                     IGVGTGSEISDFGSKIGFKGQEDLGNGLKAVWQLEQGASVAGANSGWGNKQSFVGLKG
                     GFGTIRAGSLNSPLKNTGSKVNAWESGKFTGSLLKISGMAEREHRYLSARYDSPEFAG
                     FSGSVQYAPKNNSGSNGESYHVGLNYQNSGFFAQYAGLFQRYGEGTKKIEYDGQTYSM
                     PSLFVEKLQVHRLVGGYDNNALYASVAAQQQDAKLYGTWSANSHNSQTEVAATVAYRF
                     GNVTPRVSYAHGFKGTVDDADHDNTYDQVVVGAEYDFSKRTSALVSAGWLQEGKGADK
                     IVSTASAVVLRHKF"
BASE COUNT          736 a          898 c          731 g          593 t
ORIGIN      
        1 ccgtaccggt ttttgttaat ccgctataca gcaggcgttg gtggaaatcc cgaataagcg
       61 gttcaaaacg tgtcgctgcc gcggcagcca tatacagttg gcaactgccc ggttgtgccg
      121 atgggttgcc ctgccgctcc ctgagccgcg caacggttgt ctgccttggg cgggcgcggg
      181 tggcggccgg gcggtttgtc gctatgatac ggtaattccg atatacaagg gcgggcttca
      241 gcccgccaaa tccaccaact ccgccgaatc cgccgccgtc attcccgcgc aggcgggaat
      301 ccagacctgt cggtgcggaa acttatcgga taaaacggtt tcttagattc cacatcctag
      361 attcccgcct gcgcgggaat gacgattcgg gcactcctga cagggtgaat tcacaggata
      421 gcgattcgta gcaactgcat ccaacccccc gaacaactcc ccaaacaacg ccgcgcgccc
      481 cgggcgtttg ccgtttccct gcaaaatctg cgatacaatg cagtctgaac atttatccga
      541 atcccaaacc cgatggatac cgcacaaaaa caacgctggg caataaccct atcctacgac
      601 ggcagccgct tttacggctg gcagaaacag gcgggcggcg taccgaccgt tcaggcggca
      661 ttggaaaccg cactcgcccg aatcgcagga gaatctgtcg ccaccacagt tgccggcagg
      721 accgacaccg gcgtgcacgc tgccgcccaa gtcgtccact ttgacacaac tgccgcccgt
      781 ccccaacagg catgggtgcg cggcgtaaat gcccacctgc ccgaaggcat tgccgttttg
      841 cacgcccgac aggtcgcacc cggatttcac gcccgcttcg acgcatacgg acggcactac
      901 cgctacctgc tcgaatccgc ccccgtccgt tcccccctgc tcaaaaacag ggcgggctgg
      961 acacacctca aactcgacat cgaaccgatg cgccgggctg ccgccttatt ggtcggcgaa
     1021 caagacttct ccagcttccg cgccgccggg tgccaggcaa aatcccccgt caaaaccatc
     1081 taccgcgccg ggcttaccca aagcgcagga ctgatccgcc tcgatttgca cggcaacgcc
     1141 tttttgcacc acatggtacg caacatcatg ggcgcgctcg tttatgtcgg cagcggcagg
     1201 ctcagcgtcg aaggcttcgc cgcactgatt caagaacgca gccgcctcaa agccccaccg
     1261 accttcatgc ccgacgggct ttacctgacc ggcgtcgact atcccggggc atacggcatc
     1321 gtccgccccc aaatccccga atggctttaa aacattcttg tcgcggagat tttgaaatcg
     1381 gacaaactgt caggcaatct ttttcaatgt tgacactata gcggattaac aaaaatcagg
     1441 acaaggcggc gggccgcaga cggtacaaat agtacggaac cggttcgccc ggtgcttggg
     1501 cgccttaggg aaccgttccc tttgagccgg ggcggggcaa cgccgtaccg gtttttgtta
     1561 atccgctata acccccggtt tgcaatgccc tccaataccc tcccgagtaa gtgttgtaaa
     1621 aatgcaaatc ttaaaaattt taaataacca tatgttataa aacaaaaaat acccgtaata
     1681 tctctatccg cccttcaaaa tgcacatcgg attccacaca aaaacaggca gaagtttgtt
     1741 ttttcagaca ggaacatcta tagtttcaga catgtaaccg ccgagcccct cggcggtaaa
     1801 tgcaaagcta agcggcttgg aaaacccggc ctgcttaaat ttcttaacca aaaaaggaat
     1861 acagcaatga aaaaatccct gattgccctg actttggcag cccttcctgt tgcggcaacg
     1921 gccgatgtca ccctgtacgg cgtcatcaaa gccggcgtac aaacttaccg ttctgtagaa
     1981 catcgggaag gcaaagtaat tggcgtggga accggcagcg aaatctccga cttcggttca
     2041 aaaatcggct tcaaaggcca agaagacctc ggcaacggcc tgaaggccgt ttggcagttg
     2101 gaacaaggcg cctccgtcgc cggcgctaac agcggctggg gcaacaaaca atccttcgtc
     2161 ggcttgaagg gcggcttcgg caccatccgc gccggtagcc tgaacagccc cctgaaaaac
     2221 accggcagca aagtcaatgc ttgggaatcc ggcaaattta ccggcagttt gctgaaaatc
     2281 agcggaatgg ccgaacggga acaccgctac ctgtccgcac gctacgattc tcccgaattt
     2341 gccggcttca gcggcagcgt acaatacgca cctaaaaaca attcaggctc aaacggcgaa
     2401 tcttaccacg ttggcttgaa ctaccaaaac agcggcttct tcgcacaata cgccggcttg
     2461 ttccaaagat acggcgaagg cactaaaaaa atcgaatacg atggtcaaac ttatagtatg
     2521 cccagtctgt ttgttgaaaa actgcaagtt caccgtttgg taggcggtta cgacaataat
     2581 gccctgtacg cctccgtagc cgcacaacaa caagatgcca aattgtatgg aacatggagt
     2641 gctaattcgc acaactctca aaccgaagtt gccgctaccg tggcataccg tttcggcaac
     2701 gtaacgcccc gtgtttctta cgcccacggc ttcaaaggca ctgttgatga tgcagaccac
     2761 gacaatactt acgaccaagt ggttgtcggt gcggaatacg acttctccaa acgcacttct
     2821 gccttggttt ctgccggctg gttgcaagaa ggcaaaggcg cagacaaaat cgtatcgact
     2881 gccagcgccg tcgttctgcg ccacaaattc taatctgcaa agattggtat caacaaaaag
     2941 cctgtcgcca gacaggct
//
LOCUS       DBJGGI010000116         2940 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.116, whole genome shotgun sequence.
ACCESSION   DBJGGI010000116 DBJGGI010000000
VERSION     DBJGGI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.116"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            232..810
                     /gene="grpE"
                     /locus_tag="ACSIGJ_002051"
     CDS             232..810
                     /gene="grpE"
                     /locus_tag="ACSIGJ_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HHQ1330838.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAESAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            complement(934..1149)
                     /gene="nqrM"
                     /locus_tag="ACSIGJ_002052"
     CDS             complement(934..1149)
                     /gene="nqrM"
                     /locus_tag="ACSIGJ_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HHQ1330839.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(1175..2230)
                     /locus_tag="ACSIGJ_002053"
     CDS             complement(1175..2230)
                     /locus_tag="ACSIGJ_002053"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HHQ1330840.1"
                     /translation="MPSETRLPNLIRALIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQTTADKPLRIS
                     SDFAHVTAEAIRLNRLTHGALDVTVGPLVNLWGFGPDKSVTQEPTPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIIQGGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            complement(2521..>2940)
                     /locus_tag="ACSIGJ_002054"
     CDS             complement(2521..>2940)
                     /locus_tag="ACSIGJ_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HHQ1330841.1"
                     /translation="RTDAEGYIEKLHITPANAHECKHLSPLLEGLSKGTTVYADKGYD
                     SAENRQHLEEHQLLDGIMRKACRNRPLTEAQTKRNRYLSKTRYVVEQSFGTLHRKFRY
                     ARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
BASE COUNT          652 a          665 c          845 g          778 t
ORIGIN      
        1 ggtgtgccag aggtcgaaac cggttgtgtt taaatggtct tttttcatga gtgatgacgt
       61 ttgaaaatcg atatggtcgg cagtatctta ccgtctatat tattttttcg gtaggggatt
      121 tgaaaatgaa tttgaaattc tctgcttttg cttgaagttt cttgaaaatg tccttatctt
      181 gcgcgggtaa taactggatt ttgatttcca atttgtttta agggatacga tatgagcgaa
      241 cagacacagc agcaaaacag tgaagaagcg gttgaaaatg tggaggcggt ggaaaccgtc
      301 gagacagtag gaaatgcgga cggtgtgcag gaacaggctg ccgcagagtc ggcttatgag
      361 gatttgcagg cgcggattgc cgagctggaa gcgcagttga aagacgagca gctgcgcgct
      421 ttggcaaacg agcaaaacct gcgccgccgc caccagcagg aaattgcgga tacgcacaag
      481 ttcgccggac agaagtttgc cgtggaaatg ctgccggtca aggattattt ggaaatggcg
      541 cttttggatc agagcggcaa tttcgatgcg ctgaaaatgg gcgtgcagat gactttgaac
      601 gagttgcaga aagcatttga tgctacgcaa atcaaggaaa tcaatcctaa agcgggcgat
      661 aagctcgatc cgaatatcca tcaggcgatg caggcggtgg caagcgaaca ggaacccaat
      721 accgtggtgg gcgtgatgaa gaagggttat acgctgtccg accgcgtgtt gcgcccggct
      781 atggttacgg tggcacagaa ggaagcctga aggcgtctgg ggaataatct gatttatttc
      841 ctgaagcgcg ttttgcgtat aaaccgatcg aagtaaagcg gcaatgccgt ctgaacccgc
      901 ctgtcgggct tcaggcggca ttttgtttcg gcgttaacgg tcaacccgta tgccgcctgc
      961 ctgtttttct tcatccagtt tcttttgcag ggtgtcgcaa ggtgtgtcgc agtcgcacat
     1021 ttttttcata cccaaggcag taatgccgcc gcaactgcct ttgatgctgc gtttggagaa
     1081 aatatagccg accgccatac cgatgatgac ggtcaggaag atgccgaagg taaggagcag
     1141 ggttttcatg gtgtttccta atcggtttgt atgtttagcg gagcagtttt tcaaattcgg
     1201 aagacatggc ggtgcggtag ccgcctttat ccctgacaat caggaaaaca gcgagttttt
     1261 cgcgctctgc cagctttaag gcttcggttt cgcccaatac gaataatcct gtggacaagc
     1321 cgtccgccgt catcgcactg tctgcgacca cgctgatgga ggcgaggttg tggctgatgg
     1381 gtcgtttgtt gttcggatta atgatatggg agaggcgttt gccgctttta tcgacgtgga
     1441 aaatacggta atcgccggaa gtggcaagcg aacggttgtt cagcgggacg ataatctgcg
     1501 tattgccgcc ttggatgata ttgggttgct cgataccgat gcgccacggt tcgccgcgcg
     1561 cgtttttgcc tttgccgtgc aactcgccgc cgatttcgac cagataattt tgaatgccgt
     1621 atttttccag ttcgcccgca actttatcaa cgccgaagcc tttggcaatc gaagataaat
     1681 ccaaataggc tttggggtgg gttttgctca aggaagcgta atctttgcct tgtttcaaaa
     1741 tgattttgtc tatgcccgta taagatgctg cttgtttgat ttgttccggt gtcggttcct
     1801 gggtaacgga tttgtcgggc ccgaatcccc aaaggttgac caaagggccg acggttacgt
     1861 ccagtgcgcc gtgcgtcagg cggttcaggc ggatggcttc ggcggtaacg tgtgcgaagt
     1921 cgcttgaaat gcggaggggt ttgtcggctg tggtttggtt gaaccggctg atttcggagt
     1981 cgggctgata ggtggacatc tgccggttga cttctttaag cgcgtcatcg atgcgctttt
     2041 gtatttcggc aggggaggga agtttgtccc gattgtttga aaggtatttg acggtatagg
     2101 tcgtgcccat cgtttcgcct tgcagggtaa cggtttgcgc ggtttgttcc gaacaggcgt
     2161 tcaggaagat gaaacccagg gcaaatatca aggcgcggat aaggttcggc aggcgtgttt
     2221 cagacggcat agtgtttgac ggttttggta aatggcttga attatatcgc aaaacggccg
     2281 gtatgtttct atgccgatgc cgtctgaagg gcatcagggg catcgcccgc tgtttggggg
     2341 cggtaataaa aaaacaaacc ggtgttttac aaaccgagac ctttgcaaaa ttcctgtccc
     2401 tcccgacagc cgaaacccaa acacaggttt tcggctgttt tcgccccaaa tacctcctaa
     2461 ttctacccaa ataccccctt aatccccgga tacccgataa tcaggcatcc ggaccgcctt
     2521 ttaggcggca gcgggcgcac ttagcctgtt ggcggctttc aacaggttca aacacatcgc
     2581 cttcagatgg ctttgcgcac tcactttaat cagtccgaaa taggctgccc gcgcatagcg
     2641 gaatttacgg tgcagcgtac cgaagctttg ttcgaccaca taacgggtct tcgacaaata
     2701 ccggttgcgt ttggtttgcg cttccgtcag cggacggttg cggcaggctt tgcgcataat
     2761 gccgtccaac aactgatgtt cttccagatg ttgccggttt tccgcactgt catagccttt
     2821 gtcggcatag acggtcgtac ctttggacag tccttccaac aacggcgaca ggtgtttgca
     2881 ttcatgggca ttggcggggg tgatgtgcag tttctcgata tagccttccg catcggtacg
//
LOCUS       DBJGGI010000117         2904 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.117, whole genome shotgun sequence.
ACCESSION   DBJGGI010000117 DBJGGI010000000
VERSION     DBJGGI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.117"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..306
                     /locus_tag="ACSIGJ_002055"
     CDS             <1..306
                     /locus_tag="ACSIGJ_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pretoxin"
                     /protein_id="HHQ1330842.1"
                     /translation="LSDNKSVGEGKKFTPAQKKAIIQENMNRNGGVVKSDQSGEVLVR
                     PKKSQKGITPPHQMNGRLTMCKQKVKEVQIVIKMHRFYPEEKILRNQINRYQYAMEI"
     gene            290..562
                     /locus_tag="ACSIGJ_002056"
     CDS             290..562
                     /locus_tag="ACSIGJ_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330843.1"
                     /translation="MQWKFEDPQNTAVITTRRILSKKNGILEVWHDADDGMWQFLDGN
                     EIHEGDAVIVSLKEIVTIDSSVNLLSNLPLGGYAWRNTISEKWNFD"
     gene            729..959
                     /locus_tag="ACSIGJ_002057"
     CDS             729..959
                     /locus_tag="ACSIGJ_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HHQ1330844.1"
                     /translation="METLDERIKNLGKSLEDRIDANLIDATLEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            1108..1782
                     /locus_tag="ACSIGJ_002058"
     CDS             1108..1782
                     /locus_tag="ACSIGJ_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HHQ1330845.1"
                     /translation="MVKTADGYKAIAHIQAGDRVFAKDETSGKTGYKPVTAQYGNPYR
                     ETVYIEISDGIGNNQTLISNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGNQAETEGVWVHNECPTKPKPTNHAQQRKEEAKNDSH
                     RSVGDSNRVVREGKQYLDADTGNHVYVKGDKVVILTPDGRQVTQFKNSKANTSKRVKN
                     GKWTPK"
     gene            1760..2041
                     /locus_tag="ACSIGJ_002059"
     CDS             1760..2041
                     /locus_tag="ACSIGJ_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330846.1"
                     /translation="MGNGHQNNQSSLEKRIFYLEHSGQYLMICALSDYSQNKHTVVMA
                     NFLYPNEKMDWRNLDDLFNELVLEELQSSFMDWYPTIEKAISHHLEDFS"
     gene            2115..2744
                     /locus_tag="ACSIGJ_002060"
                     /pseudo
     CDS             2115..2744
                     /locus_tag="ACSIGJ_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147002.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            2741..>2904
                     /locus_tag="ACSIGJ_002061"
     CDS             2741..>2904
                     /locus_tag="ACSIGJ_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6911 family protein"
                     /protein_id="HHQ1330847.1"
                     /translation="MKTSTIVFGGFFITDNGERIQIPILENPNIKEINNFFSVSNFEK
                     KAGVLVFRIIP"
BASE COUNT         1033 a          538 c          575 g          758 t
ORIGIN      
        1 ttatcggata ataaatctgt tggcgaagga aaaaaattta caccagctca aaagaaagca
       61 atcatccaag aaaacatgaa tagaaacgga ggggtagtga agtctgacca atctggagaa
      121 gttttggttc gtcctaaaaa gagccaaaaa ggtatcaccc ccccccatca aatgaatggc
      181 agattgacca tgtgcaagca aaaagtaaag gaggttcaaa tagttataaa aatgcacagg
      241 ttttatccag aagagaaaat attaagaaat caaataaata ggtatcaata tgcaatggaa
      301 atttgaagat cctcaaaata ctgctgttat taccaccaga agaattttgt ctaaaaaaaa
      361 cggaatctta gaagtatggc atgatgctga tgatggaatg tggcaattct tagatggcaa
      421 tgaaattcat gaaggagatg ctgtgatagt tagtttaaaa gaaattgtca ctattgattc
      481 ctcagtcaat ctcttaagta atttacctct tggtggttac gcttggagaa atactatatc
      541 ggaaaaatgg aattttgatt agcaagcgta ggttaggtta aaaaaaccaa caatcaaagg
      601 tcgtctgaaa ccgtgtttag tttttcaaac gaccttttct tcaattaaca tagaatgttg
      661 ggtctcgacc caacctacac gcttactaat ctcaaatata accttatgaa ataatgagta
      721 tttaaaagat ggaaacatta gacgaacgaa taaagaattt aggaaaatct cttgaagata
      781 gaattgacgc taatctaatt gatgccacac tagagtacat tactttttca gaacgtttgc
      841 tagcttttga aacgctatgt gattatattg aagatttcaa tatccagctt acggaaaaag
      901 aatcccaaga aatctctttt attaataagg aatttggaat tgaaagtaca tcagattaat
      961 aaatattagg tcggtttaaa aacccaacaa tcaaaaagtc gtctgaaact gtgtttaatt
     1021 tacagacgac ctttccttca tttgaagcag gatattgaga actgagttct tcaaaaatcc
     1081 tacacctgct ccttccacgg cagcaccttg gtcaaaacgg cagacggcta caaagccatt
     1141 gcccatattc aagccggcga ccgcgtcttc gccaaagatg aaaccagtgg aaaaactgga
     1201 tacaaacctg ttaccgccca atacggcaat ccgtatcggg aaaccgttta cattgaaatt
     1261 tcagacggca tcggcaacaa ccaaaccctg atttccaata aaatccaccc gttttacagt
     1321 caaggaaaat ggatacaggc aggtcgtctg aaaaaaggcg acaccctgct ttccgaaagc
     1381 ggtgcaaaac agacggttca aaacattacc ctcaaacagc agccgctcaa agcctacaac
     1441 ctgaccgttg ccgactggca tacctatttc gtcaagggta atcaggcgga aacggaaggg
     1501 gtttgggttc ataatgagtg tccgaccaaa ccaaaaccaa ctaatcatgc ccaacaaaga
     1561 aaagaagaag ctaaaaacga ttctcatcga agtgtgggag attccaatcg tgtcgttcgc
     1621 gaaggaaagc aatatttaga tgccgacaca ggaaaccatg tttatgtaaa aggagataaa
     1681 gtggttattc taactcctga tggaagacag gtaactcaat ttaagaactc gaaagccaat
     1741 acgtcaaaaa gggtaaaaaa tgggaaatgg acaccaaaat aatcaatctt ccttagaaaa
     1801 aagaattttt tatctggagc attcggggca gtatctgatg atttgcgccc tatcggatta
     1861 ctcccaaaat aaacatactg tcgttatggc gaatttcctc tatccaaatg aaaaaatgga
     1921 ttggagaaat ttagatgatt tgttcaatga gttggtttta gaggaattac agtcttcatt
     1981 tatggattgg tatccaacta ttgaaaaggc aattagccat catttagaag acttttcgta
     2041 acaagcataa atcaaaaagt cgtctgaaac tgcttttaat tttcagacga cctttcccca
     2101 tatcggcgga ttatgaattt acagctatcg ggaaattgag ttatctctct gatagagaaa
     2161 attccatacg cgaagccgtt gaccggtgga tacaggaaaa tcccaatgcc gccgaaaccg
     2221 tcgaagccgt cttcaacgtt gccgccgcca aagtcgcgaa gctggcaaag gcggcgaaac
     2281 cggggaaagc tgcggttagc ggggattttt cgattagcta taaaaacttt tctactgtca
     2341 aaccaaaagt aattgccaaa ggaacgatta atggaaagac ttttagagat gttaatcaaa
     2401 gtgcaaaaat tggatcgcct gattcaccta cattaatagc ccaacgagtt aatgcaaaaa
     2461 tccaagccga cggtaaacct cgtccgaatg caaccgttgc taattcacat gctgaaattg
     2521 gtgttatcca gcaggcttat aatgcaggtg aaacgaaagg ggcatctatg acaatgacag
     2581 tttcaggaaa agatgtttgc ggttattgta aaggagatat tgcagctgct gctcaagctt
     2641 ctggactgaa atcactgact gttaatgcga cagataatgt aacaggaaaa aataaaactt
     2701 actattggac gcctggtatg aaatcaatta aggagagaaa atgaaaacat caacaattgt
     2761 ttttggtgga ttttttatta cagataatgg agaacgaatc caaatcccta ttttagaaaa
     2821 tcctaacatt aaagaaataa ataacttttt ttctgtatca aattttgaaa aaaaagccgg
     2881 agtccttgtt ttcagaatta ttcc
//
LOCUS       DBJGGI010000118         2903 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.118, whole genome shotgun sequence.
ACCESSION   DBJGGI010000118 DBJGGI010000000
VERSION     DBJGGI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.118"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(71..412)
                     /locus_tag="ACSIGJ_002062"
                     /pseudo
     CDS             complement(71..412)
                     /locus_tag="ACSIGJ_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            410..724
                     /locus_tag="ACSIGJ_002063"
                     /pseudo
     CDS             410..724
                     /locus_tag="ACSIGJ_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(799..2853)
                     /gene="metG"
                     /locus_tag="ACSIGJ_002064"
     CDS             complement(799..2853)
                     /gene="metG"
                     /locus_tag="ACSIGJ_002064"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HHQ1330848.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVRKEHLADFTGFFIGYDNYYSTHSTENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELFNPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGIDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNGKIEDTDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRPKDVSGSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AARFLNLDAITWKNTRETLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIQTASAPVE
                     EGKYEKVAEQAGFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIAA
                     SYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGDK
                     VG"
BASE COUNT          537 a          673 c          925 g          768 t
ORIGIN      
        1 tcgggtaata cccggcaacg gctgattttt tggaagtgtt tgaaatcggc gcgtggtgtt
       61 tcgtgcaaac ggcagttgat ttatcgcggc aggttgacgg caggtgcttg gtgtcgattt
      121 tgcctttgcc gccgttgtcg gtgccggcgt cgtcggtaac gtcgtcgttg gcggcgtcgt
      181 tgcgcttaac cggctgtccg cagaaccatt ttaccgaacc ggcttcacgc ttggcccaca
      241 gggagagttt tttgtttttg atttcattgt ttacgccgct tgaaagcatt ttggcggtaa
      301 cgacgccttt ttcgaccgta acgctttcaa catatttgcc tttgatgtcg gaggcggatg
      361 ccacgccggc agcgccgttg tttgcgggcc atttgccgtt attcgggtaa tgtccggcga
      421 aaacgaattg cggcagatga agccgggtgc ggttttgatc aattgcgggc gcggcgggct
      481 ggcggatgaa aacgcgctgc ttgccgcact caaatacggg cagatcggcg gggcgggtgt
      541 cgatgttttg acggaggaac cgccgagggg cggcaatccc ttgctgaatg cacgattacc
      601 caatctgatt gttacgccgc ataccgcgtg ggcgagccgc gaggctttgg atcggttgtt
      661 tgaaatattg ttggcgaaca ttcacgcctt tgtgaaaggc gaggcgcaaa accgcgtggt
      721 ttgaacctgt cgggattgcg gaaaaaaacg ccgtctgaac gcccaagggg ttcagacggc
      781 gttttttgag atttctgcct aaccgacttt gtcgcccggc tgagcgcccg catccacgtc
      841 aagcagcttc agtttgccgt ctgccgtggc ggcggagagg atcatacctt cggatacgcc
      901 gaattttgcc attttgcgtg gggcgaagtt ggcgactgcg atgaccatgc gaccgttcaa
      961 ttcggcagga tttgggtaag acgcggcgat gccggagaag atgatgcgtt tttcaaaacc
     1021 gaaatcgagg tcgaatttca aaagtttggt gctgccttcg acggcttcgc agttcaatac
     1081 tttggcgacg cgcatatcga ttttcataaa gtcgtcgaag cccgcctgtt cggcgacttt
     1141 ttcgtattta ccctcttcaa cgggcgcgga tgccgtctga atgctttgtt tgttggcttc
     1201 gatcaaatcg tccacttgtt tttgctccac tcgttgcatt aaatgttcgt atttgttgat
     1261 ggcgtgttcg cctagggttt cgcgcgtgtt cttccaggta atcgcgtcca gattgaggaa
     1321 acgcgcggcg ttggcggcgg tttgcggcaa cacgggggcg aggtaggcgg tcagcatggt
     1381 gaaggcgttg atgagttcgc tgcatacttc gtgcaggcgt tcgtcttgac cttcttgttt
     1441 ggcgagctcc cacggcttgt tggcatcgac gtattcgttg acaatgtctg ccaatgccat
     1501 gatgtcgcgc agggcgcggg cgtattcgcg gttttcgtat tgctcggcga tggtgtcgct
     1561 ttcggcggcg agttttgcca gcaatgcgct gcctgaaacg tctttcggac gaccttcaaa
     1621 gcgtttggcg atgaaacccg aagcgcgggc ggcgatgttg acgtatttgc cgacaaggtc
     1681 gctgttgacg cgggaaataa agtcttgcag gttcaaatcg gtgtcttcga ttttgccgtt
     1741 gagcttggcg gcgatgtagt agcgcatcca ctcggggttc aggccttgtt ccagataaga
     1801 tttggcggtg atgaaggtgc cgcgcgattt ggacattttt tgtccgtcca cggtcaaaaa
     1861 gccgtgtgcg tacacgccgg tcggggcgcg gtggccggag aaatgcagca tggcgggcca
     1921 gaacagggcg tggaaataga ggatgtcttt gccgatgaag tggtacatct cggtttggct
     1981 gtcggctttg aagtattcgt cgaaatcgat gccgatgcgg tcgcacaggt ttttaaacga
     2041 cgccatatag ccgacgggcg cgtccagcca aacgtagaag tatttgcccg gcgcgtcggg
     2101 gatttcaaaa ccgaaatacg gcgcgtcgcg ggaaatgtcc cagtcggaca gggtggtttc
     2161 ttcgccttcg cccagccatt ccttcatttt gttgagggct tcgggttgca gatgaggctt
     2221 gccgtcgtgc gggttgttgc cggaagtcca tgctttgagg aagtcggcgc attcgcccag
     2281 tttgaagaag aagtgttcgg attcgcgcaa ttcgggtttc gcgccggaaa cggcggaata
     2341 cgggttgaac agttcggtcg gggaataggt cgtgccgcag acttcgcagt tgtcgccgta
     2401 ttggtcttgg gcgtggcatt tcgggcattc gcctttgacg aagcggtcgg gcaggaacat
     2461 ttgtttttcg gggtcgaaaa gctgctcgat gacgcggctt tcgattttgc cgttggcttt
     2521 cagcgcgcgg taaatgtctt gggaaaactg tttgttttca gtggaatggg tgctgtaata
     2581 attgtcgtag ccgatgaaaa agccggtaaa gtcggcaagg tgctctttgc gcactttggc
     2641 aatcatgtct tcgggcgcga tgccttgttt ttgcgcggca agcataacgg gcgtgccgtg
     2701 ggtgtcgtcg gcgcagcagt agtagcattc gtggccgcgc agtttttgaa agcgcaccca
     2761 aacgtcggtt tggatgtgtt cgaccatgtg gccgaggtgg atgctgccgt tggcataggg
     2821 cagggcggag gtaactaaga ttttgcgtgt catattgtgc tttgcaaaca atgggtaaag
     2881 gcggattata ccgcaaatca aac
//
LOCUS       DBJGGI010000119         2790 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.119, whole genome shotgun sequence.
ACCESSION   DBJGGI010000119 DBJGGI010000000
VERSION     DBJGGI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.119"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..362
                     /locus_tag="ACSIGJ_002065"
     CDS             <1..362
                     /locus_tag="ACSIGJ_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330849.1"
                     /translation="NTASGISGTAAGAAEQAFFSAAKTANRSASAASANAPPHPDKPE
                     NGRKPMTFQGHNNRKKAGGYAEYITGGELRLLQQTACRFKAALETAAWKHYVRAIKES
                     EPVPDAEARRKRKKQAA"
     gene            388..582
                     /locus_tag="ACSIGJ_002066"
     CDS             388..582
                     /locus_tag="ACSIGJ_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHQ1330850.1"
                     /translation="MDSEYLDPQQCADILSVKKRTFLERYAPRPDFPARISVSKKRFW
                     WKKEEVEGWLDRQKEKRPMM"
     gene            complement(638..722)
                     /locus_tag="ACSIGJ_002067"
     tRNA            complement(638..722)
                     /locus_tag="ACSIGJ_002067"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(686..688),aa:Leu,seq:tag)
     gene            873..1547
                     /locus_tag="ACSIGJ_002068"
     CDS             873..1547
                     /locus_tag="ACSIGJ_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330851.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGMASIDRSV
                     LWCNDEGWKLADYLPCDDVREDALKRLFMALDSVFSRSTGVRSAAVYALMPSENAALR
                     LVCLSQQGEGLENIWEQDGNITDVSLACRSAQSGWMNVASDVRRWLNLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECADTAAQAEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            1540..2133
                     /gene="ribA"
                     /locus_tag="ACSIGJ_002069"
     CDS             1540..2133
                     /gene="ribA"
                     /locus_tag="ACSIGJ_002069"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HHQ1330852.1"
                     /translation="MSELLDHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNCSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQAEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQEQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENERYLQTKADKLGHLMSE"
     gene            2301..2483
                     /locus_tag="ACSIGJ_002070"
                     /pseudo
     CDS             2301..2483
                     /locus_tag="ACSIGJ_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2486..>2790
                     /locus_tag="ACSIGJ_002071"
     CDS             2486..>2790
                     /locus_tag="ACSIGJ_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HHQ1330853.1"
                     /translation="MRNAVGLDISKLTFDATAIVGNAEYSAKFDNDSKGLNQFSDRLK
                     SLGCQNLHICMEATGNYYEEVADYFAQYYSVYVVNPLKISKYAESRFKRTKTDKQDA"
BASE COUNT          717 a          653 c          782 g          638 t
ORIGIN      
        1 caaacacggc atcaggcata agcggcacgg ctgccggcgc ggcggaacaa gcgtttttca
       61 gcgcggcgaa gacggcaaac cgcagcgcgt cagccgcatc cgcaaacgct ccgccacacc
      121 cggacaagcc agagaacgga cgaaaaccca tgacgtttca aggacacaac aaccgtaaaa
      181 aagccggcgg ctacgccgaa tacatcacgg gcggcgaact gcgcctattg caacaaaccg
      241 cctgccgctt caaagccgcc ctcgaaacgg ccgcgtggaa acactacgtc cgcgccatca
      301 aagaatccga gccggtaccg gatgccgaag cgcgccggaa gcggaaaaaa caggcggcgt
      361 aaatataatt aagtttggag gttttaaatg gatagcgaat acctagaccc gcagcaatgc
      421 gcagatattt tatccgtaaa aaagcgtaca tttttagagc ggtacgcacc gcgtccggat
      481 ttccccgcgc gaatatccgt atcgaagaaa cgtttttggt ggaaaaaaga agaagtcgaa
      541 ggatggctag accgccaaaa agaaaaacgc ccgatgatgt gatttgcacc acatttgcac
      601 caagtggtat ataacattat gaaagatata aaaattttgg tgcggacgga gagactcgaa
      661 ctctcacacc tctcggcgcc agaacctaaa tctggtgcgt ctaccaattt cgccacgtcc
      721 gcataagggt ggagattata cagattttgt ccgattgcgc aaggctttgg acgtaatagt
      781 tgaggcttat gctttgcagc ggtaaaatcc gctattcgtc cgcctggcat cggaatcggg
      841 cggttttttg tttttattga cggaatttgg gtatgcctgc tgctttgatt aaggattttc
      901 tgctgactca gggtttgaag ctgccgcttg acgaggttcg ggcggcgtat ctgacggcgc
      961 agacggtaat ggatatgggg atggcttcga ttgaccgttc tgttttgtgg tgcaatgatg
     1021 agggttggaa acttgccgat tacctgccgt gcgatgatgt ccgcgaagat gccctgaaac
     1081 ggctttttat ggctttggat tcggtgtttt cacgctcgac aggtgtgcgg agcgcggcgg
     1141 tttatgcctt gatgccgtct gaaaacgccg ccctccggct ggtgtgcctg tcccaacagg
     1201 gcgaaggttt ggaaaacata tgggagcagg atggaaatat taccgatgtt tcgcttgcct
     1261 gccgttcggc gcaaagcggt tggatgaatg ttgcctcgga tgtacgccgt tggttgaatt
     1321 tgggtgagct ttcgggagaa cgcaatcatg cttcggcggc gcaaatttcc attcccgttt
     1381 gcacggagag cggcggtgta ttgggcgtgg ttcatgtgga atttgaatgc gcagagtgtg
     1441 cggatacggc ggcgcaggcg gaatgggtgg ctcttgcctt ggctttgtcc gaacctttga
     1501 agcaactttt gggcatcact gccgcagaag gagatgaaaa tgtctgaatt attagaccat
     1561 gtcgcttcct gccgtctgcc gaccgaatgg ggcgtattta cgatgcacgg ttttgaagag
     1621 gcaaacgggc aggaacacgt cgcgctgacc gtcggcaatt gttcagacgg caatccggtg
     1681 ttgacgcgca tccactccga atgtctgacg ggcgacgcgc tgttctcgag aaaatgcgac
     1741 tgcggaccgc aacttgaagc ggcaatgagg gcggtacagg cagaggggcg cggcatcatc
     1801 gtctatctgc gtcaggaagg acgcggcatc gggctgatta acaaaatccg cgcctatcat
     1861 ctgcaagaac aaggtatgga taccgttgaa gccaatttgg cactcgggct gcccgtcgat
     1921 gcccgcgatt tccgtttggc gcaatctatc tacgaatatc tgggcatccg ctcggtcaaa
     1981 ctgttgacca acaaccccga aaaaatccaa accctgaaag atgcggggat taacgtggtc
     2041 gaacgcattc ccctgcacgt cggggaaaat ctggaaaacg agcgttatct ccaaaccaaa
     2101 gcagacaagc tggggcattt gatgtcggaa taaggcaaag ttgcagggaa cgggcatcct
     2161 gcgccgtctt tcgggaaaca ggtttccata gcttgataaa gcaataagtt ttatcaagtt
     2221 gcagggtgcg gatgcaaacg cattgcgagc gcgggtttga ggcatacgcg caaacatctt
     2281 aatacaacgt attgatattt atgattttct ctatcatcgt ccctatttac aatgtggaaa
     2341 aataccttcg ctgctgcgtg gattccgtgc ttgccgaaaa ttttgccgat tatgaaatga
     2401 ttttggtcga tgacggttcg ccggacggct gcgggaagat ttgcgacgaa tatgcaggca
     2461 aatatccgca tataagggta atcccatgcg taacgccgta ggattggaca tatccaagct
     2521 gacatttgac gcaacggcca ttgtcggcaa tgccgaatat tcggcaaagt ttgacaacga
     2581 ttcaaaaggt ttaaatcagt tttcggaccg gttgaaaagc ttgggatgtc agaatctgca
     2641 tatctgcatg gaggcaaccg gcaattatta tgaagaagtt gccgactact tcgcgcagta
     2701 ttacagcgtt tacgtagtga atccgctgaa aataagcaag tatgcagaaa gcaggttcaa
     2761 gcgaaccaaa acagacaaac aggatgcaaa
//
LOCUS       DBJGGI010000120         2785 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.120, whole genome shotgun sequence.
ACCESSION   DBJGGI010000120 DBJGGI010000000
VERSION     DBJGGI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.120"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(259..648)
                     /locus_tag="ACSIGJ_002072"
     CDS             complement(259..648)
                     /locus_tag="ACSIGJ_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HHQ1330854.1"
                     /translation="MNPPEKLLTAENPALRQRAKAMRQEMSEAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHAEQAVYDHARTVYLNSLGFTVLRFWN
                     HEILQQTNDVLAEILRVLQELEKQPAR"
     gene            complement(811..1190)
                     /locus_tag="ACSIGJ_002073"
                     /pseudo
     CDS             complement(811..1190)
                     /locus_tag="ACSIGJ_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779337.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1244..2377)
                     /gene="carA"
                     /locus_tag="ACSIGJ_002074"
     CDS             complement(1244..2377)
                     /gene="carA"
                     /locus_tag="ACSIGJ_002074"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HHQ1330855.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSASGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSSFRASESLHDYL
                     VRNETVAIADIDTRRLTMLLREKGAQGGAILTGADATVEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDKQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAEDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKQA"
BASE COUNT          569 a          697 c          797 g          722 t
ORIGIN      
        1 ggggagaggg cattctccga atggtggcaa tctttcctaa taccttcgcc gcccaaatac
       61 aagccttgcg gcttgttgcc ctctctccag ctctctccca cagggagaga ggacgggtcg
      121 gctgttgggg ttgaggtttg ctgcaaggaa aacaggttgt gcaggttgct tttttgcttt
      181 tcagacgacc tttttaaacg ctcaattcag ttaattttac cttattattt tcaaaatcta
      241 atcaaaatca accgtttgct accgtgctgg ctgcttttcc aattcctgca atacgcgcag
      301 gatttccgcc agtacatcgt ttgtctgctg caaaatttcg tgattccaaa aacgcagcac
      361 ggtaaagccc aagctgttga gatataccgt ccgcgcgtgg tcgtatacgg cttgttccgc
      421 gtgctgcccg ccgtctgctt cgacaatcag cttgggcgtt acgcacataa aatcaacaat
      481 ataattcccc atcggctgct gtcggcggaa tttatagccg ttcagacggc ctgcccgcag
      541 gtgttgccac aattttgctt ccgcctcgct catttcttga cgcatggctt tggcgcgttg
      601 gcgtagggcg gggttttcgg cggtcaatag tttctcgggc gggttcattt ttgttatcgg
      661 gttgctgttt gtgtaagcct tacagcttgt tgccctctct ctaactcttt cccacaggga
      721 gagaggacgg gtcggctgtt ggggttaaag gggctgcaag gaaaacagct tgtgcaggct
      781 gttttttatt ttgcataatc cgtctgaaaa ttaataactg ctgatttcca gcaggttgcc
      841 atcgggatcg cgcaggtaaa ccgattggat tttgcccatt gcgcctgtgc gcgctacgat
      901 gccgcttaaa ggtttgatgc cgtgtgcgga taattcctgt aaaaccgttt ccagtggggc
      961 gtatcggtca gcaggcataa atccgctgtg ccgcaggcgg cgtgttgcgc gttaggctga
     1021 atttccgcac cgcgcccgtg taggttgatt ttctgactgc caaacaacaa agctttacgg
     1081 ttgttgccaa atgaaacttc ttccatgccc aaaacttgtg tgtaaaacgc gatggttcgg
     1141 tcaatgtcgg caacagtcag tactagatgg tcgagtgcgc taattttcat ttccagcttt
     1201 ctttttcaga cggcagcagc atactgttgc cgtctgaaaa tcattatgct tgttttgcag
     1261 ccttcatatt gccaatgaat ttgtcgaaca aatagccgac atcttgcgga cccgggctgg
     1321 cttcggggtg tccttggaaa cagaacacgg gtttgtcggt cagctcgatg ccttgcaagg
     1381 tattgtcgaa caaggatttg tgggtaatgc gtgcgttggc gggcagggtg tcggcatcga
     1441 cggcgaaacc gtggttttgg ctggtaatga cgactttgcc gctgtccaaa tcttgcacag
     1501 gatggttcgc accgtggtgg ctgaagcgca ttttcagggt tttcgcgccg atggcgaggc
     1561 tgatgagctg gtgtcccaag cagatgccga aaatcggttt gccgctttcc atcagttttt
     1621 gcacggcttc gatggcgtag gtgcaaggct cggggtcgcc ggggccgttg gacaggaaca
     1681 cgccgtcggg attgagtgcc aacacgtctt ccgcgctcgt ttgtgcgggg acgacggtca
     1741 ggcggcagcc gcgcgaggcg agcatacgca ggatgttggt tttcacgccg aaatcgtagg
     1801 cgacgacgtg gtaaggctgt ttgtcagggg taacgaaacc tttgcccaat tcccattcgc
     1861 cttccgtcca ttcgtaagtt tccgtgcagg aaacttcttt tgccaaatct ttgccgacca
     1921 tgctgccgaa cgcggcgatg agttcttgcg ctttttcaac ggtggcatcc gcacctgtca
     1981 gaatcgcgcc gccttgcgcg cctttttcgc gcaacagcat ggtcaggcgg cgggtgtcga
     2041 tgtcggcgat ggcgacggtt tcgttgcgta ccaaatagtc gtgcaggctt tcggaggcgc
     2101 ggaagctgct gtgcaagagc ggcaggtcgc ggataatcag gccggcggca taaacgctgc
     2161 ggctttcttc atcttcggcg ttggtgccgg tgttgccgat gtgggggtag gtgagggtaa
     2221 cgatttgttt gcagtaggac gggtcggtca gaatttcctg atagccggtc atcgaagtat
     2281 tgaacacgac ttcgccggaa gccgaacctt cgtaaccgat tgatgtgccg tggaatacgc
     2341 tgccgtcagc gaggacgagg agggcggggg tgctcatgat gggaatcctg tttttaataa
     2401 aattcttgac aatgccgtct gaagggactt cggcaggggg catccggtcg gaaaaatccg
     2461 gtcaaaaaaa aacacgccgc gcaagataga cgcgtaacgt gcttttcggg caagtgccta
     2521 taccctgaaa gcagcatatt ttaagacgaa acatacccga tttcaagcgc aaacgcggca
     2581 ggcgggctga tatgttcgcg cgaaaacgga aatccggggc ataaaaccgc ccgctccgcg
     2641 ccccctatgc ccgttacgca tttccccacg tccgcccggc gaaactatgg gaatacccga
     2701 accgtcattc ccgcgcaggc gggaatccgg acctgtcggc acggaaactt atcgggtaca
     2761 acggtttctt gagattttac gtcct
//
LOCUS       DBJGGI010000121         2751 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.121, whole genome shotgun sequence.
ACCESSION   DBJGGI010000121 DBJGGI010000000
VERSION     DBJGGI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.121"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..528
                     /locus_tag="ACSIGJ_002075"
     CDS             <1..528
                     /locus_tag="ACSIGJ_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HHQ1330856.1"
                     /translation="ISDGIGNNQTLISNRIHPFYSDGKWIKAEDLKAGSRLLSESGRT
                     QTVRNIIVKPTPLKAYNLTVADWHTYFVKGNQAETEGVWVHNACPPRKTPSTPVYGND
                     SEAYAAAKKLGYRKIKERTRNDAAIFKKGKSYISRDVDSHNGGAWKEASSPKNLNRKE
                     TRNGTFDKNLNRIGD"
     gene            534..932
                     /locus_tag="ACSIGJ_002076"
     CDS             534..932
                     /locus_tag="ACSIGJ_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HHQ1330857.1"
                     /translation="MCEFKDFRRNIPCFEEYDENSFIGKWYDDGVWDDEEYWKLENAL
                     IEVRKKYPYPMDIPRDIVIGIGTIIDFLMVQNWKLFEIKASPWLPKSVKINERYERFR
                     VMLRYIFTDLDAEDWKFFYFPIQHSKGRLR"
     gene            963..1340
                     /locus_tag="ACSIGJ_002077"
     CDS             963..1340
                     /locus_tag="ACSIGJ_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HHQ1330858.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRRKYPYPMDIPRDIVIGIGTIIEFLMVPNWKLFEIKASPWLPDSVGINERYERLK
                     TMLRYIFTEKDIVNVQFDYYNKK"
     gene            1471..2208
                     /locus_tag="ACSIGJ_002078"
     CDS             1471..2208
                     /locus_tag="ACSIGJ_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HHQ1330859.1"
                     /translation="MVRTADGYKAIAHIQAGDRVLSKDEASGKTGYKPVTARYGNPYQ
                     ETVYIKVSDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGNRLFAESGKTQTVRNIVV
                     KPKPLKAYNLTVADWHTYFVKGDKAETEGVWVHNDCPYGGSNNLEKAKLRAERLSKND
                     RAGKDFTKAGKEAVIDLNRIQNNGQVKCANCGIETIPAKQSIKNISPTSNERQVDHVI
                     PKSKGGQGTPKNGQVLCRGCNIKKSNK"
     gene            2205..2624
                     /locus_tag="ACSIGJ_002079"
     CDS             2205..2624
                     /locus_tag="ACSIGJ_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HHQ1330860.1"
                     /translation="MKSKLTVVYYDLESNIAEEILSGNIMPDGNFLIQEIPLFAPNLA
                     LNDIVAIEREDKMLFFDHLIKASGNTTINIVVLDHFPKDLLAAIEEHSGKIRKNGENY
                     LSVNFPPKKYNSDLKGILNRYEEANILSYREACLGFS"
BASE COUNT          945 a          489 c          586 g          731 t
ORIGIN      
        1 atttcagacg gcatcggcaa caaccaaacc ctgatttcca accgcatcca tccgttttat
       61 tcggacggca aatggattaa agcggaagat ttaaaggcgg gaagcagact gttatccgaa
      121 agcggcagaa cccaaaccgt ccgcaacatc attgtcaaac caacaccgct caaagcctac
      181 aatctgaccg ttgccgactg gcatacctac ttcgtcaagg gtaatcaggc ggaaacggaa
      241 ggggtttggg ttcataatgc gtgtccgcca agaaaaacac catctacccc cgtatatggg
      301 aatgattcag aagcatatgc agcagcaaag aaattgggat atagaaaaat aaaagaaaga
      361 acgagaaatg atgctgctat ttttaaaaaa ggaaagagtt atatatcacg cgatgtagat
      421 tcacataatg gaggggcttg gaaagaagca tcttcaccca aaaatctgaa cagaaaagaa
      481 acaagaaacg gtacatttga taaaaaccta aatcgtattg gagattaatt attatgtgcg
      541 agttcaagga ttttagaaga aacatccctt gttttgaaga gtatgacgaa aattcattta
      601 ttggcaaatg gtatgatgac ggagtgtggg atgatgaaga atattggaaa ttggagaatg
      661 ctttaatcga ggttagaaaa aaatatcctt atccgatgga tataccaagg gacatcgtga
      721 ttggaatcgg taccattatt gattttttaa tggttcaaaa ttggaaactt tttgaaatta
      781 aagcttctcc ttggttgcct aaaagtgtca aaattaatga gcgttatgaa aggttcagag
      841 taatgctccg ttatattttt actgatctag atgcagagga ctggaaattt ttttactttc
      901 caatacaaca tagtaaaggt agattgagat gaaaagttta attatattgg agatacatta
      961 ttatgtgtga gttcaaggat attataagaa acgttcctta ttttgagggg tatgacgaaa
     1021 attcatttat tggcaaatgg tatgatgacg gggtgtggga tgatgaagaa tattggaagt
     1081 tggagaatga tttaatcgag gttaggagaa aatatcctta tccgatggat ataccaaggg
     1141 atatcgtgat tggaatcggt accattattg agtttttaat ggttccaaat tggaaacttt
     1201 ttgaaattaa agcttcccct tggttacctg atagcgtggg aattaacgaa cgttatgaaa
     1261 ggcttaaaac aatgctccgt tatattttta ccgagaaaga catagtcaac gtgcaatttg
     1321 attattacaa caaaaaatag caaaacccgg caatcaaaat gccgtctgaa accgtgttta
     1381 attttcaggc gaccttcccc ttattttaaa caggatattg ggaactaagt tcttcaaaaa
     1441 tcctacacct gctctttcca cggcagcacc ttggtcagaa cggcagacgg ctacaaagcc
     1501 atcgcccata ttcaagccgg agaccgcgtc ctttccaagg acgaggcaag cgggaaaaca
     1561 ggatacaaac ccgttaccgc ccgatacggc aatccgtatc aagaaaccgt ttacattaaa
     1621 gtttcagacg gcatcggcaa cagccaaacc ctgatttcca accgcatcca cccgttctat
     1681 tcggacggca aatggattaa ggcggaagat ttaaaagcgg gaaacaggct gtttgccgaa
     1741 agcggcaaaa cccaaaccgt ccgcaacatc gttgtcaaac caaaaccgct caaagcctac
     1801 aatctgaccg ttgccgactg gcatacctac tttgtcaaag gcgataaggc ggaaacggaa
     1861 ggggtttggg ttcataatga ttgtccgtat ggcggttcaa ataatttaga gaaagcaaaa
     1921 cttcgcgcag aacggcttag taaaaacgat cgggcaggaa aagactttac taaagcagga
     1981 aaagaagccg ttattgattt gaataggatt caaaataatg gtcaagttaa gtgcgctaat
     2041 tgcggtattg aaacaattcc agccaaacaa tctataaaaa atatttctcc tacttcaaat
     2101 gaaagacaag tagatcatgt catccctaaa tctaagggtg gtcaaggtac acctaaaaac
     2161 gggcaggtat tatgcagagg ctgcaacatt aagaagagta acaaatgaaa tcgaaactta
     2221 ctgtcgtcta ttacgatttg gaaagcaata ttgcagaaga aatactgtca ggaaacataa
     2281 tgcctgatgg aaattttctt attcaagaga ttcctctttt cgcaccgaat ttggctttaa
     2341 acgacattgt tgccatagaa cgtgaggata agatgctgtt tttcgaccac ttgataaaag
     2401 cttcaggaaa taccacgata aacatcgttg ttttggatca tttcccaaag gatttattgg
     2461 cagccataga agaacacagt ggtaaaatca gaaaaaatgg agagaattat ttatcggtta
     2521 attttccgcc caaaaaatat aattctgatt taaaaggaat tttaaataga tatgaggaag
     2581 caaatatcct cagctacagg gaagcttgtt tgggcttctc ttaatcgatc tataagtaag
     2641 aatggattac gttagattgt aaacctaacg attaaaaggt cgtctgaaac cgtgtttaat
     2701 tttcagacga cctatttcct tcatttgaaa caggatattg ggaattaagt t
//
LOCUS       DBJGGI010000122         2573 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.122, whole genome shotgun sequence.
ACCESSION   DBJGGI010000122 DBJGGI010000000
VERSION     DBJGGI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.122"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            1..306
                     /locus_tag="ACSIGJ_002080"
                     /pseudo
     CDS             1..306
                     /locus_tag="ACSIGJ_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
     gene            402..605
                     /locus_tag="ACSIGJ_002081"
     CDS             402..605
                     /locus_tag="ACSIGJ_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330861.1"
                     /translation="MGGQYSSLENFALSVLREKIINNTVSSDEKLLIINDFLSGFLEF
                     DPETGEPVGETLKIEQMIDFFLV"
     gene            1482..1753
                     /locus_tag="ACSIGJ_002082"
                     /pseudo
     CDS             1482..1753
                     /locus_tag="ACSIGJ_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1989..2275
                     /locus_tag="ACSIGJ_002083"
                     /pseudo
     CDS             1989..2275
                     /locus_tag="ACSIGJ_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          821 a          426 c          534 g          792 t
ORIGIN      
        1 cagaccggct ccgcttcctt tatccccgtc ccgaccgaaa ttcaggaaaa cggcagcaat
       61 accgatatgc tcgccggcac gctcggtttg cgctacggac tgaccggcaa taccgacatt
      121 tacggcagcg gcagctatct gtggcacgaa gaacgcaaac tcgacggcaa cggcaaaacc
      181 cgcaacaaac ggatgtccga catatccgcc ggcatcagcc acaccttcct caaagacggc
      241 aaaaatcccg cactcatcgc tttcctcgaa agcacggttt acgaaaaatc gcgcaacaaa
      301 gcctcgttaa tcaaaaaaag ggggctttgc cccttttata acttaagggt aaattatgaa
      361 tattaaaaat gaaatagttg ctttaatttc taaaaaaatt gatggggggg caatatagtt
      421 ctttagaaaa ttttgcttta tccgtactaa gggaaaaaat tataaacaat actgtttcca
      481 gtgatgaaaa attattgatt attaatgatt ttttatcagg ttttcttgag tttgatcctg
      541 agacagggga acctgtcggg gagacgctta aaattgagca aatgattgat ttctttttag
      601 tctaaatcat acataccagt ggacaagcta ggcgtagatc cggcttttaa atacgattgt
      661 caggtttata cttgggataa aatcaaaaat aaagttgttt aaactatagt ggattaacaa
      721 aaatcagggc aaggcgacga agccgcagac agtacagata gtacggaacc gattcactcg
      781 gtgcttcagc accttaggga atcgttccct ttgagccggg gcggggcaac gccgtactgg
      841 tttttgttaa ttcactatat tgcatggtgc atgcattaaa tcagtcttct agcgccgcaa
      901 tgggaaaaca tagaagataa acgaaatgga tgatctaata ctatattttt tatcaggtat
      961 attcggaaat caagttgctg aatatattat aaaaaacaac cgcgagataa aagttccctt
     1021 tattgcgctt tacgctatat tttttacgtt gatttatact gtggctttgt tgtttctcag
     1081 tctgatctat tgggtcaatg gtgcggagat tgcatggaag gggataggta ttttcagtat
     1141 gtcggtcagt ttttgtatag tcttctgtct ttatttggtt gacaaggcag gaagatgtaa
     1201 ggataagaaa caatagggat cagatgcggc agcaaacgta gattgggtct aggctcaacc
     1261 tgcgtttgct gcatctttgg aagaaatagt ggattcgaaa aaataggaag aattctgatt
     1321 aatggtatgc gaggtgtagc agtgggggct gttgctggtg gcataactgg ttatgcaggt
     1381 tctacgggta agaacactga cattcgccgt taaataataa ataaaccaac ttccaaccaa
     1441 acctgctatc ggtttggttg gaagtgagag gaggtaaggt agtgtttgct gttattttct
     1501 tttcgacatt gggctgtatt ttagcatgga taagagatat tcctaaaata aaatcaaaaa
     1561 aaattttggc aagatcctta tatattatag gaataataaa tgtaataatc agctatgtat
     1621 taattaaaaa tatattggtt tctgtttcgg atggaggtgg aataaaatat gttgcaatat
     1681 atttatcaaa tctttttttt ggacagtatt gatgtatgct cttgtgaaaa gattatctaa
     1741 aaagccaagc tgaaacccag tacacaacac atcaataaag attccttttg cacggaacaa
     1801 tgacaatatt caaattgctg gatttgcaat ctgatttacc tattgaaatt gggtgtgcaa
     1861 catacgttct aagcaggctt tgatttccca aagaggctga agggtaagag acgcttatat
     1921 catagataaa taatggcaaa acaggtaatg aattaaaggc attacctgtt tttacagaaa
     1981 tttttaaaat agatgatttg attgcagctt tttaggctat ttatcaggta aatacagtga
     2041 agggaaaata agcaggttag aaatatccct aacggcagga agcattttct ttgcagtatt
     2101 ctttattcat gaactgataa tttttattgt tgatggaaat aaggaaaatc tttggaattt
     2161 tattactggg ataatgaaag atttattttt attttcatgt tctgtttgtt tattattcta
     2221 tttcttatta ttgattgcag aaaccattgg aaaaacaatc aataagatta aataagatat
     2281 ttaagccgct aaaaaaccat ttgagtattt tatcttctat gcggcatcca tcttggaatt
     2341 gaaatactgc aatcgggagg ctaaggcttc cgttgtaacg tttaccatat atttcattat
     2401 tttaatttca tcaaaaaaca aaattcaaga ttatacatac cgccaatcgt ttcttgattg
     2461 ttttgtatag tggagtaaat ttaaatcagg acaaggcgac gaagccgcag acagtacaaa
     2521 tagtacggaa ccgattcact cggtgcttca gcaccttaga gaatcgttct ctt
//
LOCUS       DBJGGI010000123         2288 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.123, whole genome shotgun sequence.
ACCESSION   DBJGGI010000123 DBJGGI010000000
VERSION     DBJGGI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.123"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            361..873
                     /locus_tag="ACSIGJ_002084"
     CDS             361..873
                     /locus_tag="ACSIGJ_002084"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HHQ1330862.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLIVLPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            953..2032
                     /gene="aroB"
                     /locus_tag="ACSIGJ_002085"
     CDS             953..2032
                     /gene="aroB"
                     /locus_tag="ACSIGJ_002085"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HHQ1330863.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIITNET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFIQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGALGDIGFFEWLEQHMPELMALERAPLTQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAVEAEMGYGVWLHGEAVAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLAHMSHDKKVSGGIMRFIGL
                     NRLGEAVITEITDTDILRRTLQPYL"
BASE COUNT          559 a          709 c          578 g          442 t
ORIGIN      
        1 gaatcgttcc ctttgagcta aggcggggca acgccgtacc ggtttttgtt aatccgctat
       61 agtattgata aacatattat cttcaatata ttcaattgga taattgttta cctaagcaaa
      121 gataattgcc ttttcctgac aaattaagtg aaatcaacgg attgtcaaaa cacagcctga
      181 aataaaaaac ctccctgatt tcttttattt ttccttaaaa tcagaaaagt tcgggatggt
      241 cgggttattt ttccaaacgt accgccgccc tgccgatttc gtataaaatc ccgccgtaac
      301 ccgacaagcc cgaaccctgc cgccccgaaa ggcggggggg tcaaacatta aggaaccgtg
      361 atgaaaaact ttaacggcaa actcatcctc atcggactga tgggcgcggg caaaactacg
      421 ctgggccggc aaatggcgca gcggctggat taccgttttt acgacagcga tcacgaaatc
      481 gccgcagcgg cgggcgttcc catccccacc atatttgaaa tggaaggcga acagggattc
      541 cgttcgcgcg aaaccgccat actcaaaaaa ctgattgtcc tgccgcatat cgtcctgtcc
      601 accggcggcg gcgcggtgtt aaaagaagaa aaccgcgccc ttatccgcaa aagcggcacg
      661 gtcgtttatc tgcacgcccc gcccgaaacc ctgctcgaac gcacgcgctg cgacaacagc
      721 cgccctttgc tgcaagttgc cgatcctttg gcgaaattgc gcgaactcta cgccgcacgc
      781 gaccccgttt accgccaaac cgccgacttt accgtagaat cggcaaactg ccgggaaacc
      841 gtgcaaaccc tgctcaaacg cttatcccga taaaccggca tatgcgccgc tccccgaaaa
      901 ccaaaccgca ccccgcccgg cgggccggcg gttcaaactt taaggaacaa caatgaaaac
      961 actgaccgta cacacgcctt cgcacagcta ccccatcttt atcggcaacg ggctgctgcc
     1021 gcaggcggga agcctgctca aaccgcattt gggcaaacgc gccgccatca tcaccaacga
     1081 aaccgtcgcc ccgctctacc tcggcacgct tcagacggca ttggatgcgg caggcgtatc
     1141 ccatttcagc atcatcctgc ccgacggcga ggcgcacaaa aactggcaga cgctcaacct
     1201 catctttgac gggctgatgc aaaaccgcgc cgaacgtaaa accacgctga ttgcgctggg
     1261 cggcggcgtg atcggcgaca tggtcggctt tgccgccgcc acctaccagc gcggcgcgcc
     1321 gttcatccaa ataccgacca cgctgttgag tcaggtcgac tcatcggtgg gcggaaaaac
     1381 cgccatcaac cacccgctcg gcaaaaatat gatcggcgcg ttttaccagc cgcaggcggt
     1441 gcttgcagat ttggacacgc tgcacaccct gcccgcccgc gaactgtccg ccggtatggc
     1501 ggaagtcatc aaatacggcg cgctcggcga catcggcttt tttgaatggc tggaacagca
     1561 tatgcccgaa ctgatggcac tcgaacgcgc gcccctcacc caagccgtgt accgctgctg
     1621 ccaaatgaag gcagacatcg tcgcccaaga cgaaaccgaa cagggcatac gcgcatggct
     1681 caacctcgga cacaccttcg gacacgccgt cgaagcggaa atgggttacg gcgtatggct
     1741 gcacggcgag gccgttgccg ccggctgcgt gttggcggcg cgtttgtccg aacaactggg
     1801 caaaacctcc gccgcagata ccgcgcggct cgccgccctg ctcgaagccg ccggactgcc
     1861 gtccgcaccg cccgtgtttg cctttgaaaa atggctggca catatgagcc acgacaaaaa
     1921 agtcagcggc ggcatcatgc gctttatcgg tctgaaccgg ctgggcgaag ccgtcatcac
     1981 cgaaattacc gacacggaca tcctccggcg caccctgcaa ccgtatctct gatttcctct
     2041 gccgatgtgt tgccgcgcgg gtttgacgca cgatgatgac tttccatcat cattctccgc
     2101 gaaagcgggc atccggttat tccatattgc agggatttat ccggaataac gacaacctgt
     2161 ccgccgtcat ttccgcgcag atggaaattt ggaaattcaa aatcacagga atttatccgg
     2221 aattatagcg gattaacaaa aatcaggaca aggcggcgaa gccgcaggca gtacggatgg
     2281 tacggaac
//
LOCUS       DBJGGI010000124         2247 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.124, whole genome shotgun sequence.
ACCESSION   DBJGGI010000124 DBJGGI010000000
VERSION     DBJGGI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.124"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(121..1265)
                     /locus_tag="ACSIGJ_002086"
                     /pseudo
     CDS             complement(121..1265)
                     /locus_tag="ACSIGJ_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980936.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            complement(1590..2066)
                     /gene="greA"
                     /locus_tag="ACSIGJ_002087"
     CDS             complement(1590..2066)
                     /gene="greA"
                     /locus_tag="ACSIGJ_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HHQ1330864.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPAEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
BASE COUNT          540 a          584 c          540 g          583 t
ORIGIN      
        1 ccgcttttct tccgtatccg gatttttgtt tggggctgaa gcagattggc agtcagattg
       61 caatcgaaga atgaaggcaa gccatcaaaa acaaagctat ccgcttcacc gccccgatat
      121 ttagaatttg tggcgcagac caacggaggc ggcattaatt tgagtgtagt tgccgatgcc
      181 ggtatttcgt ttcagccaag cggcagacat gatggcggaa gtgcgcttgg aaaaatcgta
      241 atcgacaccg gcgatgattt gatcatagct ggtatgttcg cgtttctgac tgcgttcgac
      301 aaagtcgaaa ccatgggcat agctgatgcg cgggactgta ttaccgaagc ggtaggaagc
      361 agtggcggca atttcggtcg tactgttttt ggttttgtcg gcattttcag acaaatccaa
      421 ctgagccgcc aaggcgagat tcaagccgcc ttccccatag ccgcccgtca ggcggtgtac
      481 ctgatggttt ttcaatggat cggtatcact cgctctgccg agcaagaaca aaaagaaagc
      541 atcatgcccc tcattggcgt gtttcgcata tttaagggca taatttccga aaaagccgcc
      601 atttttgtaa ttcagaccgg cataatacac atccgacccc ggcttgccga caacagccgg
      661 aactggtttc atctgattac tttccagcgt gaaagtagca ggcgtatagg cggacttgct
      721 gttttgactc ggaacaaatt gaatgctgcc gctgaaaccg gaaaatccgg gggaatcgta
      781 acgcacggaa accggcatac cgtcgtggcg tttgaaaata cccaattgcg aagccacatt
      841 attattgctg tcccaaggat caatggcttt gctggcatcg ccaaacggat tcgcaacgcg
      901 accggcgagc gccgtgccga attcgcctgc caagccgata aaggattccc tgttacccca
      961 acgggtcgcg ccgccgccgg caacggatac gtcttgctcg agctgccaaa cagccttcag
     1021 cccgccgccc aaatccccac ccccttaaag ccgataaacg agccgaaatc actgactttc
     1081 gtcctgatgc ggcttttggc cttagtaact gtattgcccg tttgaccttc tgagggtggc
     1141 tcggtcaact gcagccggat gttcctgcct tccacaccag ctttgacttc gccgtacagg
     1201 ctgacatcgg caactgccgc aaacggcagt gcggacaata cgagggcggt aagttttttt
     1261 cgcatatcgg cttccttttg taaatttgct aaaaacatcg ggcaaacacc caatcagtct
     1321 ccaattaccc cccgaaaaaa aaaccatttt tcggaacaaa tttcgggtaa attttggaat
     1381 ttttattttg aaaaataata tttcaatata aaacaataaa ttaacttttt tgaaaatgtt
     1441 gctttcccgc aacatgattt aaatacagac cttgtcaaag ccgatgcggc gcagacccgg
     1501 atgccgtctc gttttttaaa acaccataaa aaaacacacg cggtacttta atttcctgcg
     1561 tgtgtcgagt gtatcgaaat caagccgaat caaatatacc ggacttcgat aatatcgtat
     1621 tcgcgtacgc cgcccggtgc ctgaacttcc gccgtatccc cttcttcctt gccgatcagg
     1681 gcgcgggcaa tcggagagcc gacatagatt ttgccctgtt tgatgtcggc ttcgtcttcg
     1741 ccgacaattt gataggtaac gtgttcttcc gtttccaaat cttccagcgt aaccgtcgta
     1801 ccgaacacga ttttgccttc ggcgtggatt tcggccggat tgatgatgtg ggcaacggaa
     1861 agtttgtgtt ccaactcgga aatgcggccc tcgataaaac cttggcgttc tttggcggct
     1921 tcgtattcgg cgttttcgga caaatcgccg tgcgatctgg cttcggcaat cgcttcgatc
     1981 acttcgggac gcgccacgct tttgagctgc tgcaattcct gtttcagcaa ttccgcaccg
     2041 cgtacggtca gggggatttt ttgcatcggt ctttttctcc atattccggc acaccggttt
     2101 gcggcggcaa gcataccgcg taccgtcttg ttttgtgcgt ccggatatta aaataaaaat
     2161 acaagccgcc cggaaaatcg gcggcttgtc tgtcgttgaa cagcggctat tctaccaaat
     2221 tctatgaaat tggcaatcgt gccgtgc
//
LOCUS       DBJGGI010000125         2203 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.125, whole genome shotgun sequence.
ACCESSION   DBJGGI010000125 DBJGGI010000000
VERSION     DBJGGI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.125"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..1113
                     /gene="mafB"
                     /locus_tag="ACSIGJ_002088"
     CDS             <1..1113
                     /gene="mafB"
                     /locus_tag="ACSIGJ_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HHQ1330865.1"
                     /translation="HGHEVHSPFDNHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPED
                     GYDGPRGGGYPPPGGARDIYSYHIKGTSTKTKINTVPQAPFSDRWLKENAGAASGFLS
                     RADEAGKLIWENDPDKNWRANRMDDIRGIVQGAVNPFLTGFQGLGVGAITDSAVNPVT
                     YAAARKTLQGIHDLGNLSPEAQLAAASLLQDSAFAVKDGINSARQWADAHPNITATAQ
                     TALAVAEAATTVWGGKKVELNPTKWDWVKNTGYKKPAARHMQTVDGEMAGGNRPLESK
                     NTVTTNNFFENTGYTEKVLRQASNGDYHGFPQSVDAFSENGTVIQIVGGDNIVRHKLY
                     IPGSYKGKDGNFEYIREADGKINHRLFVPNQQLPEK"
     gene            1129..1431
                     /locus_tag="ACSIGJ_002089"
     CDS             1129..1431
                     /locus_tag="ACSIGJ_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm40 family immunity protein"
                     /protein_id="HHQ1330866.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPTAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSINKAMHYLSQIKLENAYVSFVLKFI"
     gene            1515..1865
                     /locus_tag="ACSIGJ_002090"
                     /pseudo
     CDS             1515..1865
                     /locus_tag="ACSIGJ_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            1866..2126
                     /locus_tag="ACSIGJ_002091"
     CDS             1866..2126
                     /locus_tag="ACSIGJ_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HHQ1330867.1"
                     /translation="MRPTTRDELRQALQEQGFRRTGSDAAQYETWKGPDGVKIDIRPN
                     GEVIRTQRVPRTDGVQGKYPQRQDYEGNPLPNNHHHSGYFVK"
BASE COUNT          743 a          416 c          498 g          546 t
ORIGIN      
        1 cacggacacg aagtacacag tccgttcgat aatcatgatt caaaaagcac ttctgatttc
       61 agcggcggtg tagacggcgg ttttaccgtt taccaacttc atcggacagg gtcggaaatc
      121 catccggagg atggatatga cgggccgcga ggcggcggtt atccgccccc cggaggagca
      181 agggatatat acagctacca tatcaaagga acttctacaa aaacaaagat aaacactgtt
      241 ccgcaagccc ctttttcaga ccgctggcta aaagaaaatg ccggtgccgc ttccggtttt
      301 ctcagccgtg cggatgaagc aggaaaactg atatgggaaa acgaccccga taaaaattgg
      361 cgggctaacc gtatggatga tattcgcggc atcgtccaag gtgcggttaa tcctttttta
      421 acgggttttc agggattggg agttggggca attacagaca gtgcggtaaa cccggtaacc
      481 tatgcggcag cacggaaaac tttacagggt attcacgatt taggaaattt aagtccggaa
      541 gcacaacttg ccgccgcgag cctattacag gacagtgcct ttgcggtaaa agacggcatc
      601 aattccgcca gacaatgggc tgatgcccat ccgaatataa cagcaacagc ccaaactgcc
      661 cttgccgtag cagaggccgc aactacggtt tggggaggta aaaaagtaga acttaacccg
      721 accaaatggg attgggttaa aaataccggc tataaaaaac ctgctgcccg ccatatgcag
      781 actgtagatg gggagatggc aggggggaat agaccgctgg aatcaaaaaa tactgtaaca
      841 acgaataatt tttttgaaaa taccggttat acagagaaag tattaaggca agcatcgaat
      901 ggtgattatc atggttttcc gcaatcagta gatgcttttt ctgaaaatgg aacagtaata
      961 caaatagttg gcggagataa tattgtaagg cataaacttt atatccctgg atcttataaa
     1021 ggaaaagatg gaaattttga atatatcaga gaagcagatg gaaaaattaa tcatagatta
     1081 tttgtcccta accaacaact tcctgaaaaa tagtagatag agattaatat gttaaatgaa
     1141 atttttgaaa tttattcgag acaaggggaa tctttgatag gaattggaat tagagaagcc
     1201 gcattacccg tccccactgc aatagatata ttaaatttat ttataaatga aagaatactt
     1261 gtattggggg gagatattta tatcaagaaa gataattatt tttatcaaac atatgataat
     1321 tggtattacg agggaagtaa tttatttaac agtatcaaca aagcaatgca ttatttatct
     1381 caaataaaat tagagaatgc atacgtgtct tttgttttga aatttatcta acaaaggaag
     1441 cacaagaata ggtttatagt aaaacatcaa gatgttgaaa atgctgggtt ttaacctaac
     1501 ctacgctgac cggatcagat acagccgctc agcagactct acaaggtatt aatgatttag
     1561 gaaatttaag tccggaagca caacttgccg ccgcgagcct attacaggac agtgcctttg
     1621 cggtaaaaga cggcatcaat tccgccagac aatgggctga tgcccatccg aatataacag
     1681 caacagccca aactgccctt gccgtagcag aggccgcagg tacggtttgg agaggtaaaa
     1741 aagtagaact taacccgact aaatgggatt gggttaaaaa taccggctat aaaaaacctg
     1801 ctgcccgcca tatgcagact ttagatgggg agatggcagg aggaaacaag ccagttgtta
     1861 aatctattag accaactacg cgagatgaat tacgtcaagc attgcaagaa caaggtttta
     1921 gacgtactgg ttcagatgcg gctcaatatg aaacatggaa aggtcctgat ggcgtgaaaa
     1981 tagatattcg tccaaatgga gaggttataa gaacccaaag agtgccgcga accgatggtg
     2041 tacagggaaa atatccgcaa cgacaagatt atgaaggcaa tccattgcca aataatcatc
     2101 atcattctgg atattttgtc aaatgaaaaa aaatattttt cacaatgtaa gcctttatga
     2161 aataatcttt tccgataatg gaaataccct tacattatct ttt
//
LOCUS       DBJGGI010000126         2195 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.126, whole genome shotgun sequence.
ACCESSION   DBJGGI010000126 DBJGGI010000000
VERSION     DBJGGI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.126"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(126..1094)
                     /gene="ispH"
                     /locus_tag="ACSIGJ_002092"
                     /gene_synonym="lytB"
     CDS             complement(126..1094)
                     /gene="ispH"
                     /locus_tag="ACSIGJ_002092"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HHQ1330868.1"
                     /translation="MNGKTIILANPRGFCAGVDRAISIVERALEEFGAPVYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQNCEIIMIGHKGHAEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNASYLQRTWFEGKSKVGVTAGASAPEVLV
                     REVLAAIRGWGHETVREGGGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1124..1618)
                     /gene="lspA"
                     /locus_tag="ACSIGJ_002093"
     CDS             complement(1124..1618)
                     /gene="lspA"
                     /locus_tag="ACSIGJ_002093"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HHQ1330869.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFAALGKIGAAM
                     IIGGASGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKD
                     SKKT"
BASE COUNT          493 a          626 c          569 g          507 t
ORIGIN      
        1 ggaagaaggt gctcatggga aatcccctaa atgccttggt gggaatttag gggattttgg
       61 ggatttttgc aaaggtctcg gcatttgtat tgcccaaaca tccgatgcct gcgttaacgc
      121 ctgcgtcaac gtttgcacaa atcgggtttg gtttcgccct cgcggcgcag ctccttgggc
      181 aggacgaaca cgatgctttc ttccgcgccc cccccttcgc gcacggtttc atgcccccat
      241 ccgcgtatgg ctgccaatac ttcccgcacc aacacttcgg gcgcggacgc gcctgccgtt
      301 acgccgactt tgcttttgcc ttcaaaccac gtgcgttgca ggtaggacgc gttgtccacc
      361 atatacgcat cgattccgcg cgatgccgcc acttcgcgca ggcggttgct gttggacgaa
      421 ttgggcgaac cgaccacaat cacgatgtcg cactgttccg ccagctcttt gacggcggtt
      481 tgccggttgg tcgtcgcata gcagatgtct tccttgtgcg gattgcggat attggggaaa
      541 cgcgcgttca gcgcggcgat gatgtctttg gtttcatcga ccgagagcgt ggtttggctg
      601 acataggcga gtttgtcggg gtttctgact tcgagttttg ccacatctcc gaccgtttcg
      661 accaaaagca ttttgcccgg tgcaagctgc cccatcgtgc cttcgacctc ggcgtgcccc
      721 ttatgcccga tcatgatgat ttcacagttt tgggcatcca gtcgggcgac ttccttatgc
      781 actttcgtta ccagcgggca agtcgcatca aatacccgga aaccgcgctc cgccgcttcc
      841 tgctgcaccg ccttcgatac gccgtgtgcc gaataaacca gtgtcgcgcc cggcggcact
      901 tccgccaagt cttcgataaa caccgcgcct ttttcgcgca ggttgtccac gacgaatttg
      961 ttgtggacga cttcgtggcg cacataaacc ggcgcgccga attcttccaa agcacgttcg
     1021 acaatactga ttgcccgatc cacaccggcg cagaagccgc gcggattggc aaggatgatg
     1081 gtttttccgt tcataagttt tgcattccgt gttcagacgg cattcacgtt tttttgctat
     1141 ctttgcgatg gacgatattg tcaagcaccg ccaacaccgc accgacgcag ataaagctgt
     1201 cggcaatatt aaaggcggga taaaaccaat tttgccaata aaacaataag aaatcgacga
     1261 catgaccgtg tatcaggcga tcgatgacat tgcccgacgc accgccgata atcattgccg
     1321 ccccgatttt gccgagggct gcaaactcgt cgcgcaagat ggcgcgtacc aaatacgcgc
     1381 tcaccgccac cgccagcacc aaaaaaaagt atttctgcca gccgccctga tcggcaagga
     1441 agctgaatgc cgcgcccggg ttgtacacca gcgtcagatc gaaaaacgaa gggatgacgt
     1501 tgacgcgttc ccgatactga aacgacgaca gcaccgccca cttcgaccac tggtccagca
     1561 cgatggcggc aagtgccaat acccaatagc gcgttttact tgaaacagat gaagacatat
     1621 ttttcaacag ccggataaaa gagtgccatt ctacccgaaa accccctttc ctgtacccga
     1681 aacggcaaat gccgtaatct tgaaacccgt cattcccgcg caggcgggaa tccggacctg
     1741 tccgcacgga aacttatcgg ataaaacggt tgcccaaacc ccgcgtccta gattcccact
     1801 ttcgcgggaa tgacggttcg gttgcgtagg gccgggtggt cgaaaaggcg gattcgatgg
     1861 attcgatgaa aacggtagac atgttggatt gatggaaatg gcgggccgaa gcacacccta
     1921 cggcccgccg gatttccgcc tgcgcgggaa tgacggggtt tcaggttgct gtttcaggtt
     1981 tctgttttta agtttctgtt tcggtttgct attttataga aatgacgaaa ttttagattg
     2041 caagaactta tcccctccgc cgtcattccc acggaagtgg gaatctagaa ataaaaagca
     2101 gcaggaattt atcggaaata actgaaaccg aacggaccgg attcccgcct gcgcgggaat
     2161 gacgaatcca tccgcacgga aacctgcacc acgtc
//
LOCUS       DBJGGI010000127         2159 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.127, whole genome shotgun sequence.
ACCESSION   DBJGGI010000127 DBJGGI010000000
VERSION     DBJGGI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.127"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            1..344
                     /locus_tag="ACSIGJ_002094"
                     /pseudo
     CDS             1..344
                     /locus_tag="ACSIGJ_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            503..1054
                     /locus_tag="ACSIGJ_002095"
     CDS             503..1054
                     /locus_tag="ACSIGJ_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330870.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSLWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCIIGLV
                     GRFRLQTASGRVAKAGGAVKAGG"
     gene            complement(1319..1909)
                     /locus_tag="ACSIGJ_002096"
     CDS             complement(1319..1909)
                     /locus_tag="ACSIGJ_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HHQ1330871.1"
                     /translation="MLGLCLLTPASYQNIHVRSWKARRDSGIVKQDLDFSCGAASIAT
                     LLNNFYGRHYSEAEILDKMDKTQMRASFDDMQRIMPELGFEAQGYALPFEQLVQLKIP
                     VIVYLKYRKNNHFSVLNGINGETVLLADPSLGHVSTSKSQFLSAWKTRDGEMEGKILA
                     IVPKNTDFVRNQMFFNKNPVRQTRFTVEQIQMRQKR"
BASE COUNT          571 a          400 c          545 g          643 t
ORIGIN      
        1 ctttatttcc tattatccgt gaacaagtga aacctgacag tattgtttat acggattgtt
       61 atcgtagcta tgatgtatta gatgtgagcg aatttagcca ttttagcttc gctgaaactt
      121 cgttttcgta tcaatcacag cacacatttt gccgaacgac aaaaccatat taatggaatt
      181 gagaactttt ggaatcaggc aaaacgtcat ttacgcaagt ttaacggcat tcccaaagcg
      241 cattttgagc tgtatttaaa ggagtgcgaa tggcgtttta acaacagtga gataaaagtt
      301 cttgttccat tttaaaacaa ttagtaaaat cgagtttatc ctagttatct aggacagccc
      361 cttatttttt gttcggcggc ttgcgtggtc gggtatagtg gattaaattt aaatcaggac
      421 aaggcgacga agccgcagac agtacaaata gtacggcaag gcgaggcaac gccgtactgg
      481 tttaaattta atccactata aaatgaaggt tttgaacggt tggtcagata ggaagatgtg
      541 gcgggttttg agtgctttgc cgataggcgt ggtgtttttt gatttgatct acggttttgt
      601 gttgaatgtg ttgcaggggt tggatttgca gcgtgccgtg ccggattcgg aaggcgtgtt
      661 ggcggttacg cccgatattg cgttcaacag tttgcagatt gtcgccaacg gcggtatggc
      721 ggcggtggtc tgtttcgggt tggcggttgt gtttttgctc aaccgttcgg tgcggcggcg
      781 gcaggtcttg gaaatcgggg tgttccggat gttggggctg gtggcggtat tggcgttcag
      841 cgcgccttcg ctgtgggagt gggcgaacgc gctgccgctg ctgctgaagg gcgcggacgt
      901 ggtcaatacg gggaatgcgc gttatgtgct gacggctttg tgtatgccct ttccggcggt
      961 gtcgtgcatc atcgggctgg tagggcggtt caggcttcag acggcatcgg gcagggtggc
     1021 aaaggcaggg ggtgcggtca aggcgggcgg atagggcgca tttttcagcg ggtgcgtcga
     1081 gaagcagccg atgtgtttgg cagccgcagc ttgggggggt gtagtgctaa tggcggtttc
     1141 tttgctttta tagtggatta acaaaaacca gtacggcgtt gcctcgcctt agctcaaaga
     1201 gaacgattct ctaaggtgct gaagcaccaa gtgaatcggt tccgtactat ttgtactgtc
     1261 tgcggcccgc cgccttgtcc tgattactat ataaaataaa tgggcaaaaa tcggtttatt
     1321 atcgtttttg ccgcatttgg atttgttcta ccgtaaaacg tgtttgacga acgggatttt
     1381 tattaaaaaa catctgattt ctaacaaaat cagtattttt tggcacgatg gctaaaattt
     1441 ttccttccat ttcgccatca cgtgttttcc atgcgctcaa gaattgtgat ttgctcgttg
     1501 agacgtgccc cagcgatgga tcagccagca aaacagtttc tccgttaata ccgttcaata
     1561 ccgaaaaatg gttgttttta cggtatttta aatacacaat tacaggaatt tttagttgta
     1621 ccaactgttc aaatggcaaa gcataacctt gtgcttcaaa acccagttcg ggcattatgc
     1681 gttgcatatc atcaaaagaa gcacgcattt gggttttatc cattttgtct aagatttccg
     1741 cttcagaata atgtctgcca taaaaattat tcagtaacgt ggcaatcgaa gccgcgccgc
     1801 aagaaaaatc caaatcttgt tttactatgc cggaatctcg ccgtgctttc caactccgta
     1861 catggatgtt ttggtaagaa gcgggggtca acaaacatag gccaagcaaa aactatattt
     1921 ggggcgaaac caatcaaagc cgcataattt atcaatttat aaagattttt tatcataata
     1981 tgtatacgcg gaataaacgc atgaaacata aaaaaataaa atcatatgca attttattgc
     2041 atgaataaat atgaataaaa tagataatga tgggagtaaa tacgccatgt attttggaag
     2101 tttaaattta ttaataataa aataatttat cgtagcgcaa ataaatccca aaattggaa
//
LOCUS       DBJGGI010000128         2077 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.128, whole genome shotgun sequence.
ACCESSION   DBJGGI010000128 DBJGGI010000000
VERSION     DBJGGI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.128"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            31..1056
                     /gene="mafB"
                     /locus_tag="ACSIGJ_002097"
                     /pseudo
     CDS             31..1056
                     /gene="mafB"
                     /locus_tag="ACSIGJ_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
     gene            1428..1901
                     /locus_tag="ACSIGJ_002098"
     CDS             1428..1901
                     /locus_tag="ACSIGJ_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HHQ1330872.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYENKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNIFELKPVLEDLASEMRDYSPKNWLYILLNDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSNHSYEENISRLYFNWGKYLSNKSRSG"
BASE COUNT          617 a          548 c          496 g          416 t
ORIGIN      
        1 gtcatccgcc gccgcaaagg aggataaacc gtgaaaccgc tgcgcagact catcaagctc
       61 cttgccgcct gtgccgtagc ggcggccgca ctcatacagc ccgccctcgc ggcggacttg
      121 gcgcaagacc cgttcattac cgataacacc caacggcagc actacgaacc cggcggcaaa
      181 taccacctct tcggcgaccc gcgcggcagc gtttccgacc gcaccggcaa aatcaacgtc
      241 atccaagact atacccacca gatgggcaac ctgcttatcc aacaggcaaa catcaacggc
      301 acaatcggct accacacccg cttttccgga cacggacacg aagaacacgc ccccttcgac
      361 aaccacgccg ccgacagcgc aagcgaagaa aaaggcaacg ttgacgacgg ctttaccgtg
      421 taccggctca actgggaagg acacgaacat catcccgccg atgcctacga cggcccgaag
      481 ggcggcaatt accccaaacc tacgggcgca cgagacgaat acacctatca cgtcaacggc
      541 acagcccgca gtatcaaact caatccgacc gacacccgca gcatccggca acgcatatcc
      601 gacaactaca acaacctcgg cagcaatttc tccgaccgcg ccgatgaagc caacagaaaa
      661 atgttcgagc acaatgccaa gctcgaccgc cggggcaaca gcatggagtt tgtcaacggc
      721 gtcgccgccg gcgcgctcaa cccctttatc agcgcgggcg aagccttggg cataggcgac
      781 atactgtacg gaacgcgcta tgccatagac aaagccgcaa tgcgcaacat cgcccccttg
      841 cccgccgagg gtaaattcgc cgtcatcggc ggcttgggca gcgcggcggg ctttgaaaag
      901 aatacgcgcg aagccgttga ccggtggata caggaaaacc ccaatgccgc cgaaaccgtc
      961 gaagccctgg tcaacatcct gccgttttcc aaagtcaaag gattggtgaa ggcggcgaaa
     1021 ctggggaggg cggcaaaacc ggggagggct gcgaaactag ggaaggcaaa ggttagcgag
     1081 gattttgctg ttgccagtca ggcaagatat acttcgcaaa cggcagaaaa tataattgca
     1141 gccgatagaa taggaagtgg attaaaagct gatacgtcac atcgtgcagc cagctttcta
     1201 tctcaagaac aattgggagc aggaagaaca ttctcaatta ctggcggcga tggtattcaa
     1261 agaactttat tgcagacaaa aggttctttg aacggtcaaa gcggtatata tgaatatatc
     1321 ctcgattcaa ataacaaagt gactcatcaa agattcatta aaggaggtga aattacagga
     1381 caaccaaacc aacgcgtgaa aactaaagta cgcaaccggg ggaaaaaatg aaccttgatt
     1441 taaccgctca aaaagtccgc ctttcttgga aggatattct gtgggggtat gagaataaat
     1501 acttgggttg ggctgatgtg gcagcttatg cccgaaaaat gacgctttca gatcatgatg
     1561 aacgcgtgtt caagctatct ttaatcaaca aatccaatat ttttgaatta aagcctgttc
     1621 tagaagattt ggcttcggaa atgagggatt attcccctaa aaattggctg tacatcctcc
     1681 taaatgacgt attccataga aaagaagaat ttgaggatcc tctgggggaa gttgaaaaaa
     1741 tttatgcgga ttttgattac ccggaagaaa tagaatcatt tgtcaggtat atgccgccca
     1801 aagacggcta tatcccttct aatcatagtt atgaagaaaa tatttctcgg ttatatttta
     1861 attggggaaa atatttgagc aacaagagta ggtcgggtta aaaattcaac aatcaaaagg
     1921 tcgtctgaaa ctgcttttaa ttttcaggcg accttcccct tattttaaac aggatattgg
     1981 gaactaagtt cttcaaaaat cctacacctg ctccttccac ggcagcacct tggtcaaaac
     2041 ggcagacggc tacaaagcca ttgcccgtat ccgggtc
//
LOCUS       DBJGGI010000129         1856 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.129, whole genome shotgun sequence.
ACCESSION   DBJGGI010000129 DBJGGI010000000
VERSION     DBJGGI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.129"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            249..1028
                     /locus_tag="ACSIGJ_002099"
     CDS             249..1028
                     /locus_tag="ACSIGJ_002099"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HHQ1330873.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGKGFIWRAYSVVSAEYADTLEYFAVLIQDGPMSALFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYGHSFRFVPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKLTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            1237..>1856
                     /locus_tag="ACSIGJ_002100"
     CDS             1237..>1856
                     /locus_tag="ACSIGJ_002100"
                     /EC_number="2.7.7.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHQ1330874.1"
                     /translation="MAAHNKLTQKQIEAAKADGKQSKLADGGGLYLLLHPNGSKYWRM
                     RYRHGGCEKTLALGVYPAVSLKQARELARAARAQTAAGIDPVAEHHRTRPGSGRSLPE
                     IARAWYGSRQGQRADNTLEGDRRSLAYLTDYYKDNTDINDITTAGVSKFIEHLNRINI
                     PACARRTVQILAQIWDYAVQRGIITDERRNPATTARPLLNTSKPKP"
BASE COUNT          551 a          600 c          370 g          335 t
ORIGIN      
        1 aaaagaaaaa ttacattctt ttgatagata tttttattat tataaaccta taaactgtag
       61 gccgaaactt ttgtaaaact gtccatactc attaccgcct agtcggtaat gcatttttta
      121 gtagtatttg aaaaagtatg atgataaaaa cggttaagcc taactttttc ataaaatcca
      181 gcagagtaag gcagaaaaaa acacccaaat tggctataat cccgacaaac acactcaagg
      241 acaacaacat ggcagcctcg cccgaagcaa aattcaccga agaaaagatt ttgtgggtca
      301 aacaccacac gccgaaactc atcactttcg ccatcagccg tcccgaatcc taccgtttta
      361 aagccggaca gttctcccga ctcggtttct acgaaggtaa aggctttatt tggcgtgcct
      421 attccgtcgt ttccgcagaa tatgccgaca cgctcgaata ttttgccgta ctcatccaag
      481 acggccccat gtcggccctt ttcgccaaaa tgcaacaggg cgacaccatc ctgctcgata
      541 aaaatgccac tggctttctc ctgcccgaac gcttccccga cggcaaggat ttggtgatgc
      601 tctgtaccgg ctcgggcatc gcccccttcc tttccattct cgaacaaccc gaaatccggc
      661 agcgtttcga taccgtcaac ctgatacatt ccgtatcttt tcccgaagaa ttgattttca
      721 acgaccggct ggccgcattg tccgaacatc ccctggtagg cgaatacgga cactcgttcc
      781 gtttcgtccc tgttaccacc cgtgccgcca acccctcggg cttaagcgga aaacgcattc
      841 cggaactctt aaaaaacaac agcatcgaac aggcgctgca taccaagctt accccggaat
      901 ccacacggtt tatgatttgc ggtaacccgg aaatggtcaa agacactttc caaacgctgc
      961 tcgatatggg ttacgccatg caccgcaacc gcattcccgg tcaaatcatg atggaaaacg
     1021 gcttctaaaa acgaacgctc ccaaccccta cccacgccca cacacaaaac aaaataaaaa
     1081 acagcgatat acaaaaacca atttgcaaaa acagacctca cgcctatact gccactcaca
     1141 gcaagcaaca caaaaaagcg ggtacaaatt agggtacaaa acacaagccc gaacacagaa
     1201 gcaccccaac caaacccaaa ggcaaaggca aacaaaatgg cggcacacaa caaactgacc
     1261 caaaaacaaa tagaagcagc caaggcagac ggcaaacaaa gcaagctggc cgacggcggc
     1321 ggcttatatc tgctgctgca ccccaacggc agcaaatact ggcggatgcg ctaccggcac
     1381 ggcggatgcg aaaaaaccct agccttgggc gtatatcccg ccgtcagcct caagcaggcc
     1441 cgcgaacttg cccgcgccgc ccgggcgcag accgccgccg gcatcgaccc cgtcgccgaa
     1501 caccaccgca cccgtcccgg cagcgggcgc agccttcccg aaatcgcccg cgcgtggtac
     1561 ggcagccgtc aagggcaacg cgccgacaac acactggaag gcgaccgccg ttcactcgcc
     1621 tacctgaccg actactacaa agacaacacc gacatcaacg acatcaccac cgccggcgta
     1681 tcaaaattta tcgaacacct caaccgcatc aacatccccg cctgcgcccg gcgcaccgtc
     1741 caaatcctcg cccaaatatg ggactatgcc gtccagcgcg gcatcattac cgacgaacgg
     1801 cgcaaccccg caaccaccgc ccgcccactg ctaaacacaa gcaaacccaa acccca
//
LOCUS       DBJGGI010000130         1779 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.130, whole genome shotgun sequence.
ACCESSION   DBJGGI010000130 DBJGGI010000000
VERSION     DBJGGI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.130"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            50..244
                     /gene="thiS"
                     /locus_tag="ACSIGJ_002101"
     CDS             50..244
                     /gene="thiS"
                     /locus_tag="ACSIGJ_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HHQ1330875.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQKPFAVAVNTVFIPKGAY
                     TETVLHENDKIDIVRPVVGG"
     gene            323..1111
                     /locus_tag="ACSIGJ_002102"
     CDS             323..1111
                     /locus_tag="ACSIGJ_002102"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HHQ1330876.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRR
                     TGCGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALKILRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARTKAQASTPTVGQPFWHSAEY"
     gene            1126..1338
                     /locus_tag="ACSIGJ_002103"
     CDS             1126..1338
                     /locus_tag="ACSIGJ_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HHQ1330877.1"
                     /translation="MPSEGFRRHRGPKRANRHSPASRLCREEQGNRPENLAARPDTAT
                     NETPGLPGAQAAAQALSTAIEGCFVV"
BASE COUNT          421 a          559 c          443 g          356 t
ORIGIN      
        1 cgtaccggtt tttgttaatc cgctataaat acagagacat cgagaaacca tgaacatcat
       61 cttaaacggc ggacccgccg aacttcacgg cacaagcgtt gccgacctca tcgcccaaac
      121 cgcgccgcaa aaaccctttg ccgtggcggt caacaccgtt ttcatcccca aaggcgcgta
      181 tacggaaacc gttttacacg aaaacgacaa aatcgacatc gtccgccccg tcgtcggcgg
      241 ctgaatgccg tctgaaaccc caaaggcacg ccgccggctt tcagacggca tcgcgtccga
      301 caaccatacc aaagaatcca tcatgctcac cctgtacggc gaaactttcc cttcgcggct
      361 gctgctcggc acagccgcct acccgacccc tgaaatcctc aaacaatccg tccgaaccgc
      421 ccggcccgcg atgattaccg tctcgctgcg ccgcacggga tgcggcggcg aggcgcacgg
      481 tcaggggttt tggtcgctgc ttcaagaaac cggcgttccc gtcctgccga acacggcagg
      541 ctgccaaagc gtgcaggaag cggtaacgac ggcgcaaatg gcgcgcgaag tgtttgaaac
      601 cgattggata aaattggaac tcatcggcga cgacgacacc ttgcagccgg acgtgttcca
      661 actcgtcgaa gcggcggaaa tcctgattaa agacggcttc aaagtgttgc cttattgcac
      721 cgaagacctg attgcctgcc gccgcctgct cgatgcgggc tgtcaggcgt tgatgccgtg
      781 ggcggctccc atcggcacgg gtttgggggc ggttcacgcc tatgcgctca aaatcctgcg
      841 cgaacgcctg cccgacacgc cgctgattat cgacgcgggc ttgggtttgc cttcccaagc
      901 ggcacaagtg atggaatggg gttttgacgg cgtattgtta aacaccgccg tttcccgcag
      961 cggcgacccc gtcaacatgg cgcgcgcctt cgcactcgcc gtcgaatccg gacggctggc
     1021 atttgaagcc gggccggtcg aagcgcgaac caaagcccaa gccagcacgc cgacagtcgg
     1081 gcaaccgttt tggcattcgg cggaatattg aaaaaggcgg caaaaatgcc gtctgaaggc
     1141 ttcagacggc atcgcggccc caaacgggca aaccgccatt ccccggcatc acggctttgt
     1201 cgtgaggaac agggaaaccg gccggaaaac cttgccgccc gccccgacac cgcaaccaac
     1261 gaaacacccg gactccccgg cgcgcaggct gctgcgcaag ccctaagtac ggcaatagaa
     1321 gggtgttttg tcgtttgaat acatttaaaa atacctcggg gcatccgtaa gccttcatcc
     1381 gcaacggttt caccgctttt gcatccccga atccgcgctc aaacaccccc gaatggcaac
     1441 cctgtccgca ccaaatcgga cggttgttca aacacggcag gcttctttcc gacaggcacg
     1501 aagcccccgt ccatgcctgc cgaccccgat ttgtccggcg caatgaaagt ttgccgaccc
     1561 aaatcacaaa catcggcgga caggttaatt tgtttatttt tcatcgcatt acaaaaaatc
     1621 cgcatttatt tttaaatttt tattgataat tattattatt agcgtataat caaaaccact
     1681 cggaagccgt ccgttccgaa ccattaaaca ctgtattccc tcacaattca ctttcacact
     1741 tggagtcggc atatacgaga catacattcc ctttttaat
//
LOCUS       DBJGGI010000131         1765 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.131, whole genome shotgun sequence.
ACCESSION   DBJGGI010000131 DBJGGI010000000
VERSION     DBJGGI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.131"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..262
                     /locus_tag="ACSIGJ_002104"
     CDS             <1..262
                     /locus_tag="ACSIGJ_002104"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330878.1"
                     /translation="QPCRLQNQHRHAGARFCEMREGAECGGKSDVLPANQSVRRRGVS
                     DGIGGGSRKKDGGAWGISPRNAPNLSGKAGKTLWEYPSLPEI"
     gene            301..795
                     /locus_tag="ACSIGJ_002105"
     CDS             301..795
                     /locus_tag="ACSIGJ_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HHQ1330879.1"
                     /translation="MTVQNTQTETVRTEAAPQQGGNTNPGYYKNRAFECVGFAQYLNF
                     NLGNAFKYIWRHKEKGGREDLEKALRYLERQRADAPKFKKLKCRRYEKMYAGLKDCGF
                     DGGTEAALLAVISAAYYIRDGEDNFAWAAACVEDLLEKMPPEAGRAPHPESPMPPETA
                     GGGI"
     gene            972..1121
                     /locus_tag="ACSIGJ_002106"
     CDS             972..1121
                     /locus_tag="ACSIGJ_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330880.1"
                     /translation="MDTLLSIITALSFAGAATLAVWLLVEASDAVLRRKRDGKGEDDF
                     DGFGY"
     gene            1150..1431
                     /locus_tag="ACSIGJ_002107"
     CDS             1150..1431
                     /locus_tag="ACSIGJ_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330881.1"
                     /translation="MAEEMRTCKACGGTKPLEKGFNAVPRKEGGVYYYKSCKTCRNKA
                     VRQKRAEKRAAAGAGAMTAARLHGYIRAAHAACPILGAGLWTQPAGECA"
     gene            1422..>1765
                     /locus_tag="ACSIGJ_002108"
     CDS             1422..>1765
                     /locus_tag="ACSIGJ_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HHQ1330882.1"
                     /translation="MRVIRLILPYPVSANRYWRIWRNRAVRSAEAAAYRETVRRIAQG
                     AGAMPSEGAVAVYVRLIPKANKDGGANKTVIDLDNALKVTLDALQGVAYHNDRQVRRI
                     AADYADEPVAGGG"
BASE COUNT          441 a          501 c          555 g          268 t
ORIGIN      
        1 gcaaccatgc cgacttcaaa accaacaccg gcacgccggt gcgcggtttt gcgaaatgcg
       61 cgaaggcgcg gaatgcggag gaaaaagcga cgtactaccc gcgaaccaat ccgtgcgccg
      121 ccggggcgtt tcagacggca tcgggggcgg cagtcgcaaa aaggacggcg gtgcttgggg
      181 aatatccccc cgcaatgcgc cgaatttgag cgggaaggcg ggtaaaacgc tttgggaata
      241 tcccagccta cccgagattt aaaaaacgcg ttaaaacgca aatttgaaag gaaatacgga
      301 atgacggtgc aaaacacgca aaccgaaacc gtccggacgg aagccgcgcc gcaacaaggc
      361 ggcaatacca acccgggcta ttacaaaaac cgcgccttcg agtgcgtcgg gtttgcgcaa
      421 tacctcaact tcaacctcgg caacgccttc aaatacatct ggcggcacaa ggaaaaaggc
      481 gggcgcgaag acttggaaaa agccctgcgg tacttggaac gccaacgcgc cgacgcgccg
      541 aagttcaaga aactcaaatg ccgccgctat gaaaaaatgt acgccggtct gaaagattgc
      601 gggttcgacg gcggcacgga ggccgcgctg cttgccgtca tctccgccgc ttattacatc
      661 cgcgacggcg aagacaattt tgcgtgggcg gccgcctgtg tcgaagattt gttggaaaaa
      721 atgccgcctg aagcggggcg ggccccgcac cctgaaagcc cgatgccgcc tgaaacggcg
      781 ggcggaggca tttgacccgc caacccgacc gccgccattc ccgcgaaagc gggaatccgg
      841 aatcccggac tttcagataa tctttgaata ttgctgttgt tccaaggtcc ggattcccgc
      901 ctgcgaggga atgacggagg cggcgggaat ccgaccccga cccataaaac cgaccgaaag
      961 gaaataaaac aatggatacc ctgttaagca tcatcaccgc gctgtcgttt gccggggcgg
     1021 cgacgttggc ggtatggctt ttggtggaag cctccgacgc ggttttgcgc cgcaagcgcg
     1081 acggcaaagg cgaagacgac ttcgacggct tcggatatta aacacataaa aacaaaagga
     1141 aaaatcaaaa tggcggaaga aatgcgcacc tgcaaggcct gcggcgggac caagccgttg
     1201 gagaaagggt ttaatgccgt cccgcgcaag gaaggggggg tctattatta caaatcgtgc
     1261 aaaacctgcc gcaacaaggc agtccggcaa aagcgcgcgg aaaaacgcgc ggcggcggga
     1321 gccggcgcga tgacggcggc aaggctgcac ggatacatcc gcgccgcgca cgccgcctgc
     1381 ccgatattgg gcgccggcct gtggacgcaa ccggcagggg aatgcgcgtg atacgcctta
     1441 tcctgcctta ccccgtatcg gcaaaccgat attggcggat ttggcgcaac agggcggtca
     1501 ggagcgcgga ggcggcggcg tatagggaaa ccgtccgccg tatcgcgcaa ggggcgggcg
     1561 cgatgccgtc cgaaggcgcg gttgccgtat atgtgcggct gatacccaaa gcgaacaaag
     1621 acggcggcgc aaacaagacg gtgatcgatt tggacaacgc cctgaaggtt acgctggacg
     1681 cgcttcaagg cgttgcctat cacaacgaca ggcaggtgcg gcgcattgcc gccgattacg
     1741 ccgacgagcc ggtcgcaggc ggcgg
//
LOCUS       DBJGGI010000132         1657 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.132, whole genome shotgun sequence.
ACCESSION   DBJGGI010000132 DBJGGI010000000
VERSION     DBJGGI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.132"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..668
                     /locus_tag="ACSIGJ_002109"
     CDS             <1..668
                     /locus_tag="ACSIGJ_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HHQ1330883.1"
                     /translation="EVLSEEKDGNRLARHHKHGKIWKKPTRHEDIAAFFPFGNPIHNN
                     TMIMRRSVIDGGLRYDTERDWAEDYQFWYDVSKLGRLAYYPEALVKYRLHANQVSSKH
                     SVRQHEIAQGIQKTARNDFLQSMGFKTRFDSLEYRQTKAAAYELPEKDLPEEDFERAR
                     RFLYRCFKRTDTPPSGAWLDFAADGRMRRLFTLRQYFGILYRLIKNRRQARSDSAGKE
                     QEI"
     gene            668..1507
                     /locus_tag="ACSIGJ_002110"
     CDS             668..1507
                     /locus_tag="ACSIGJ_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HHQ1330884.1"
                     /translation="MQNHVISLASAAERRAHIAATFGSRGIPFQFFDALMPSERLERA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGVPYVAVFEDDVLFGKDAEKFLA
                     EDTWLEERFDKDSAFIVRLETMFMHVLTSPSGVADYGGRAFPLLESEHWGTAGYIISR
                     KAMRFFLDRFAVLPPERLHPVDLMMFGNPDDREGMPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRRLNRKQQWRDSPANTFKHRLIRALTKIGREREKRRKRREQTIGKIIV
                     PFQ"
     gene            1519..>1657
                     /locus_tag="ACSIGJ_002111"
     CDS             1519..>1657
                     /locus_tag="ACSIGJ_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HHQ1330885.1"
                     /translation="MDIVFAADDNYAAYLCVAAKSVEAAHPDTEIRFHVLDAGISEEN
                     RA"
BASE COUNT          411 a          446 c          459 g          341 t
ORIGIN      
        1 tggaagtttt gtcggaagaa aaggacggca accggctggc gcggcaccac aaacacggca
       61 aaatttggaa aaagccgacc cggcacgaag acatcgccgc ctttttccct ttcggcaacc
      121 ccatacacaa caacacgatg attatgcggc gcagcgtcat tgacggcggt ttgcgttacg
      181 acaccgagcg ggattgggcg gaagattacc aattttggta cgatgtcagc aaattgggca
      241 ggctggctta ttatcccgaa gccttggtca aataccgcct tcacgccaat caggtttcat
      301 ccaaacacag cgtccgccaa cacgaaatcg cgcaaggcat ccaaaaaacc gccagaaacg
      361 attttttgca gtctatgggt tttaaaaccc ggttcgacag cctagaatac cgccaaacaa
      421 aagcagcggc gtatgaactg ccggagaagg atttgccgga agaagatttt gaacgcgccc
      481 gccggttttt gtaccgatgc ttcaaacgga cggacacgcc gccctccggc gcgtggctgg
      541 atttcgcggc agacggcagg atgaggcggc tgtttacctt gaggcaatac ttcggcattt
      601 tgtaccggct gattaaaaac cgccggcagg cgcggtcgga ttcggcaggg aaagaacagg
      661 agatttaatg caaaaccacg ttatcagctt ggcttccgcc gcagaacgca gggcgcacat
      721 tgccgcaacc ttcggcagtc gcggcatccc gtttcagttt ttcgacgcac tgatgccgtc
      781 tgaaaggctg gaacgggcaa tggcggaact cgtccccggc ttgtcggcgc acccctattt
      841 gagcggagtg gaaaaagcct gctttatgag ccacgccgta ttgtggaaac aggcattgga
      901 cgaaggcgta ccgtatgttg ccgtatttga ggatgatgtc ctgtttggca aagacgcaga
      961 aaagttcctt gccgaagata cttggttgga agagcgtttt gataaggatt ctgcctttat
     1021 cgtccgtttg gaaacgatgt ttatgcacgt cctgacctcg ccctccggcg tggcggacta
     1081 cggcgggcgc gccttcccgc tgttggaaag cgaacactgg gggacggctg gctatattat
     1141 ttcccgaaag gcgatgcgtt ttttcttgga caggtttgcc gttttgccgc ccgaacgcct
     1201 gcaccctgtc gatttgatga tgttcggcaa ccctgacgac agggaaggaa tgccggtttg
     1261 ccagctcaat cccgccttgt gcgcccaaga gctgcattat gccaagtttc acgaccaaaa
     1321 cagcgcattg ggcagcctga tcgaacatga ccgccgcctg aaccgcaaac agcaatggcg
     1381 cgattccccc gccaacacat tcaaacaccg cctgatccgc gccttgacca aaatcggcag
     1441 ggaaagggaa aaacgccgga aaaggcgcga acagacaatc ggcaaaatta ttgtgccttt
     1501 ccaataaaag gagaagagat ggacatcgta tttgcggcag acgacaacta tgccgcctat
     1561 ctttgcgttg cggcaaaaag cgtggaagcg gcccatcccg atacggaaat caggttccac
     1621 gtcctcgatg ccggcatcag tgaggaaaac cgggcgg
//
LOCUS       DBJGGI010000133         1643 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.133, whole genome shotgun sequence.
ACCESSION   DBJGGI010000133 DBJGGI010000000
VERSION     DBJGGI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.133"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            2..1489
                     /gene="mafB"
                     /locus_tag="ACSIGJ_002112"
     CDS             2..1489
                     /gene="mafB"
                     /locus_tag="ACSIGJ_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HHQ1330886.1"
                     /translation="MRRLTNLLAACAVAAAAFGQPALAADLAQDPFITDNAQRQHYEP
                     GGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHGHEE
                     HAPFDNHAADSASKEQGNVDEGFTVYRLNWEGHEHHPADGYDGPQGGNYPKPTGARDE
                     YTYNVNGVARSIKLNPTDTRDVRQRISDNYSNLGSNFSDRADEANRKMFEHNAKLDRW
                     GNSMEFINGVAAGALNPFISAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEGKFAVI
                     GGLGSVAGFEKNTREAVDRWIQENPNAAETVEALVNVLPFAKVKNLTKAAKPGKAAVS
                     GDFSAAYNTRTTRKVTTETEGLNRIRQNQKNSNIHEKNYGRDNPNHINVLSGNSIQHI
                     LYGDEAGGGHLFPGKPGKTTFPQHWSASKITHEISDIVTSPKTQWYAQTGTGGKYIAK
                     GRPARWVSYETRDGIRIRTVYEPATGKVVTAFPDRTSNPKYNPVK"
     gene            1504..>1643
                     /locus_tag="ACSIGJ_002113"
     CDS             1504..>1643
                     /locus_tag="ACSIGJ_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HHQ1330887.1"
                     /translation="MKTLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DY"
BASE COUNT          510 a          450 c          379 g          304 t
ORIGIN      
        1 gctgcgcaga ctgacaaacc tccttgccgc ctgcgccgta gcggccgccg ccttcggaca
       61 gcccgccctc gcggcggact tggcgcaaga tccgttcatt accgataacg cccaacggca
      121 gcactacgaa cccggcggca aataccacct cttcggcgac ccgcgcggca gcgtttccga
      181 ccgcaccggc aaaatcaacg tcatccaaga ctatacccac cagatgggca acctgctcat
      241 ccaacaggca aacatcaacg gcacaatcgg ctaccacacc cgcttttccg gacacggaca
      301 cgaagaacac gcccccttcg acaaccacgc cgccgacagc gcaagcaaag aacaaggcaa
      361 cgttgacgaa ggctttaccg tataccgcct caactgggaa gggcacgaac atcatcccgc
      421 cgacggctat gacggcccgc agggcggcaa ttaccccaaa cctacgggag cacgcgacga
      481 atacacctat aacgttaacg gcgtagcccg cagcatcaaa ctcaatccga ccgacacccg
      541 cgacgtcaga caacgcatat ccgacaatta cagcaacctc ggcagcaatt tctccgaccg
      601 cgccgatgaa gccaacagaa aaatgttcga gcacaatgcc aagctcgacc gctggggcaa
      661 cagcatggag tttatcaacg gcgtcgccgc cggcgcgctt aaccccttta tcagcgcggg
      721 cgaagccttg ggcataggcg acatactgta cggaacgcgc tatgccatag acaaagccgc
      781 aatgcgcaac atcgccccct tacccgccga gggcaaattc gccgtcatcg gcggcttggg
      841 cagtgtggcg ggctttgaaa agaatacgcg cgaagccgtt gaccggtgga tacaggaaaa
      901 ccccaatgcc gccgaaaccg tcgaagccct ggtcaacgtc ctgccgtttg ccaaagtcaa
      961 aaacctgaca aaggcggcaa aaccggggaa ggctgcggtt agcggggatt tttctgctgc
     1021 atacaataca agaacaacta gaaaagttac tacagaaaca gaggggttaa atagaatcag
     1081 acagaaccag aaaaatagta atatacatga gaaaaattat ggaagagata atcctaatca
     1141 tattaatgtt ttatctggaa attctataca acatatactg tatggagatg aagcaggagg
     1201 tgggcatctt tttcctggca aacctggtaa gacaacattc ccccaacatt ggtcagccag
     1261 taaaataact catgaaatta gtgatatcgt tacatcccca aaaacgcaat ggtatgcaca
     1321 gactggaaca ggcggcaaat atattgctaa aggaagacca gccaggtggg tatcatatga
     1381 aacgagagat ggaattcgta tcagaacagt ttatgaacct gcaacaggaa aagtggtaac
     1441 tgcattcccc gatagaacct ctaatcccaa atataaccct gtaaaataat gagtatttga
     1501 aagatgaaaa cattagacga acgaataaaa aatttaggaa aatctcttga agatagaatt
     1561 gacgctaatc taattgatgc cgcactagag tacattactt tttcagaacg tttgctagct
     1621 tttgaaacgc tatgtgatta tat
//
LOCUS       DBJGGI010000134         1505 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.134, whole genome shotgun sequence.
ACCESSION   DBJGGI010000134 DBJGGI010000000
VERSION     DBJGGI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.134"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            53..322
                     /locus_tag="ACSIGJ_002114"
     CDS             53..322
                     /locus_tag="ACSIGJ_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330888.1"
                     /translation="MSVKELFKVILDKNKDFPIRTIHRTPMGILPKPVALSIVQYEND
                     PGFYLFYLDETGQEQTDTYHDTLDSAFEQAEFEFGISKEEWMQSP"
     gene            304..456
                     /locus_tag="ACSIGJ_002115"
     CDS             304..456
                     /locus_tag="ACSIGJ_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330889.1"
                     /translation="MDAKSLDGKIKMPLYFSDGISVNKKVGIGAQNSLRLAGIGMKSL
                     RKNDYS"
     gene            443..988
                     /locus_tag="ACSIGJ_002116"
     CDS             443..988
                     /locus_tag="ACSIGJ_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HHQ1330890.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLDNEVDNLSDMKRKPFAW"
BASE COUNT          486 a          260 c          323 g          436 t
ORIGIN      
        1 tggacgtgta atagaaaata gtgtagtaac aattattcag gattagaaaa ttatgagtgt
       61 aaaagaatta ttcaaagtta tcttagataa aaataaagat tttcctatta gaacgattca
      121 tcgcactcca atggggatac ttcccaaacc tgttgcttta agtattgttc aatatgaaaa
      181 tgacccggga ttttatttgt tttatttgga tgagaccggt caggaacaaa cggatactta
      241 tcatgacaca ttagattcgg catttgaaca ggctgaattt gaatttggaa tcagcaaaga
      301 ggaatggatg caaagtcctt agacggcaaa atcaagatgc cgctttattt ttcagatggc
      361 atttccgtca ataaaaaagt cggtattgga gcccaaaaca gcctacgtct tgccgggata
      421 gggatgaaat ctttaagaaa aaatgattat agttgaacat ttagaacatt atttgggtga
      481 aatcgaaagc ggtataaaat gcttggatcg acgttatcat ctcagcgttt ccgtcttccc
      541 atcacaacca tataaagggg taactacttt ttctacttta ggcttgaatc gatatgactt
      601 gaactataag agtaggtttg aattaatttt tacctgttct gaagaatgga ataaagaaaa
      661 tattgcagct tttctatcag gagtggctga atatttgatt gacaatagac aacctatttt
      721 aagaggtgaa attattcaat tacccagggt aattatcgaa ggtagcaaaa tggatgcact
      781 gtatgtttcc gccccatttt atttcgatga tgattttcag gtctgttatg gcgaacacta
      841 caatatcgtt ttccctttgc ttgtcccctt gtataaacag gaagcagaat tggtggaaaa
      901 gaaaggttgg aatgcttttg agcagttctt gctggataat gaagttgaca acctttcgga
      961 tatgaagcgg aaaccgtttg cttggtaagg gctttcttaa atttgattaa aggtcgtctg
     1021 aaattaagct tgtttttcag atggtctttc cttcatttga agcaggatat tgagaactga
     1081 gttcttcaaa aatcctacac ctgctccttc cacggcagca ccttggtcaa aacggcagac
     1141 ggctgaaaag caaacaccgt ccgtcgtgtt gccgtttgcg gatgagtacg ggtcaacccc
     1201 aatgccgccg aaaccgtcga agccgccttc aacattgcca ccgccaaagc cgcgaagttg
     1261 gcaaaaacgg taaaaccggg gagataaaag ccgatggcag gaaagtaaat gtgaggatag
     1321 acagtacgga ggcagacctg ctttatccgg cagggcaata agaaaacaaa acttagatat
     1381 ggaaaacgat tgtgaagatt aaaccattac aattttctaa caataatcac agattttatg
     1441 tggacaaaat ttgtgttacc gaacaagatg tgaacatttt ggcatccgac cgaaattatg
     1501 aatta
//
LOCUS       DBJGGI010000135         1398 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.135, whole genome shotgun sequence.
ACCESSION   DBJGGI010000135 DBJGGI010000000
VERSION     DBJGGI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.135"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(125..1048)
                     /gene="lpxC"
                     /locus_tag="ACSIGJ_002117"
     CDS             complement(125..1048)
                     /gene="lpxC"
                     /locus_tag="ACSIGJ_002117"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HHQ1330891.1"
                     /translation="MLQRTLAKSIGVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLNPYLINDTRLSSTIVTDKGLRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSPPTFEIDFAGKSYIGEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IGDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAVNNALLRAV
                     LADETAYEWVEFADSDDLPDAFHELNIRNCG"
BASE COUNT          332 a          341 c          383 g          342 t
ORIGIN      
        1 aatccgggtc tgtcggcacg gaaacttatc gggaaaacgg tttctttaga ttttacgtcc
       61 tagattcccg cccgcgcggg aatgacggca tctgtttgac ggcatttgtt tggaaaaggg
      121 ggcgttatcc gcaatttctg atgttcagct cgtgaaatgc gtcgggcaaa tcatcgctgt
      181 cggcaaattc cacccattcg taagccgttt cgtctgccaa aactgcgcgc aaaagggcgt
      241 tgttgacggc gtgtcccgat ttgtagcctt caaacgcgcc gacaatcgga tgtccgacga
      301 tatacaaatc gccgatggcg tcgaggattt tgtggcgcac aaactcatcg ggatagcgca
      361 agccttcagg attcaggaca tccgtgtcgc caatcacgat ggcgttgttc aaattgccgc
      421 ctaagccgag attgtgggcg cgcatcattt ccacttcgtg cataaagccg aaagtgcgcg
      481 cgcgcgcgat ttcgccgatg taggatttgc cggcgaaatc aatttcaaac gtaggcgggc
      541 tgcggttgaa aacaggatgg tcgaattcga tggtcagcgt taccttaaaa ccgtcatacg
      601 gcgtaaatcg aacccatttg cccgcttctt tgatttcgac gggtttgagg attttcaaaa
      661 aacgcttttg cgccttttgg tctacaacgc ccgcatcttg caggaggtaa ataaacggca
      721 ggctggagcc gtccataatc gggatttcgg gcgcgttcaa ctcaatcagc gcattgtcga
      781 tgccgtaggc ggacagtgcg gacatgatgt gttcgatggt gccgacgcgc aggcctttgt
      841 cggtaacgat ggtggatgaa aggcgggtat cgttgatcaa ataagggttc agcttgattt
      901 gttcgcccat ctcgccgtcc aaatcggtgc ggcggaagga aatcccgctg ttttcaggcg
      961 cagggtgcag ggtcagcgcg acgcgttcgc ccgaatgcag cccgactccg gtaacgccga
     1021 tggatttcgc caaagttctt tgcagcataa accgcttcct tatcaatggg gtaagtttcg
     1081 gaataatacg ataaaaccgg aaaaacaggc tatgtttttc aatagtattt gccaatgtat
     1141 ccgtttccaa cacgtaagcc gcatgaaaat gtatggcgga ttaacgaaaa tcaggacaag
     1201 gcggcgggcc gcaggcagta cggatagtac gaaaaggcga ggcaacgccg tactggttta
     1261 aatttaattc actatacatc ccgtcattcc cgcgcgggcg ggaatctaga atcccggact
     1321 ttcagataat ctttgaatat tgctgttgtt ctaaggtccg gattcccgcc tgcgcgggaa
     1381 tgacgaatcc atccgtac
//
LOCUS       DBJGGI010000136         1386 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.136, whole genome shotgun sequence.
ACCESSION   DBJGGI010000136 DBJGGI010000000
VERSION     DBJGGI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.136"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            113..1075
                     /gene="mafA"
                     /locus_tag="ACSIGJ_002118"
     CDS             113..1075
                     /gene="mafA"
                     /locus_tag="ACSIGJ_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HHQ1330892.1"
                     /translation="MRARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVEADNSHEGYGYSDEAVRQHRQGQP"
     gene            1115..>1386
                     /locus_tag="ACSIGJ_002119"
     CDS             1115..>1386
                     /locus_tag="ACSIGJ_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HHQ1330893.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNGRGSVKNRVCAVQTFDATAVGPILPITHERTGFEGIIG"
BASE COUNT          397 a          333 c          316 g          340 t
ORIGIN      
        1 aatttccatt ttaaaacaat tagtaaaatc gagtttatcc tagttgtcca ggacggcccc
       61 taatttattt acaattttga tacaatttgt ttttcatcaa aggagaaaat ctatgcgggc
      121 acggctgctg atacctattc ttttttcagt ttttatttta tccgcctgcg ggacactgac
      181 aggtattcca tcgcatggcg gaggcaaacg cttcgcggtc gaacaagaac ttgtggccgc
      241 ttctgccaga gctgccgtta aagacatgga tttacaggca ttacacggac gaaaagttgc
      301 attgtacatt gcaactatgg gcgaccaagg ttcaggcagt ttgacagggg gtcgctactc
      361 cattgatgca ctgattcgcg gcgaatacat aaacagccct gccgtccgta ccgattacac
      421 ctatccgcgt tacgaaacca ccgctgaaac aacatcaggc ggtttgacgg gtttaaccac
      481 ttctttatct acacttaatg cccctgcact ctcgcgcacc caatcagacg gtagcggaag
      541 taggagcagt ctgggcttaa atattggcgg gatgggggat tatcgaaatg aaaccttgac
      601 gaccaacccg cgcgacactg cctttctttc ccacttggta cagaccgtat ttttcctgcg
      661 cggcatagac gttgtttctc ctgccaatgc cgatacagat gtgtttatta acatcgacgt
      721 attcggaacg atacgcaaca gaaccgaaat gcacctatac aatgccgaaa cactgaaagc
      781 ccaaacaaaa ctggaatatt tcgcagtaga cagaaccaat aaaaaattgc tcatcaaacc
      841 caaaaccaat gcgtttgaag ctgcctataa agaaaattac gcattgtgga tggggccgta
      901 taaagtaagc aaaggaatca aaccgacgga aggattgatg gtcgatttct ccgatatccg
      961 gccatacggc aatcatacgg gtaactccgc cccatccgta gaggctgata acagtcatga
     1021 ggggtatgga tacagcgatg aagcagtgcg acaacataga caagggcaac cttgattcac
     1081 actgccataa ccgcttgctg ccaaggaaaa caaaatgaat ttgcctattc aaaaattcat
     1141 gatgctgttt gcagcggcaa tatcgttgct gcaaatcccc attagtcatg cgaacggttt
     1201 ggatgcccgt ttgcgcgatg atatgcaggc aaaacactac gaaccgggtg gcaaatacca
     1261 tctgttcggt aatggtcgcg gcagtgttaa aaatcgggtt tgcgccgtcc aaacatttga
     1321 tgcaactgcg gtcggcccca tactgcctat tacacacgaa cggacagggt ttgaaggcat
     1381 tatcgg
//
LOCUS       DBJGGI010000137         1358 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.137, whole genome shotgun sequence.
ACCESSION   DBJGGI010000137 DBJGGI010000000
VERSION     DBJGGI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.137"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(12..290)
                     /locus_tag="ACSIGJ_002120"
     CDS             complement(12..290)
                     /locus_tag="ACSIGJ_002120"
                     /inference="COORDINATES: protein motif:HMM:NF025063.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HHQ1330894.1"
                     /translation="MAISDVFYQKQLKGNGLVQSLSRKGNCLDNAAMESFFGTLKSEC
                     FHTCKYDSVTESEAALHEYIRYYNNDRIKLKLKGLSPVQYRIQSLKAA"
     gene            complement(254..832)
                     /locus_tag="ACSIGJ_002121"
     CDS             complement(254..832)
                     /locus_tag="ACSIGJ_002121"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HHQ1330895.1"
                     /translation="MPSVELLLGIVGLPRSTFYYQLAVQSAEGKYADLKRHIHDIYQR
                     YKGRYGYRRIAAAIRHAGIPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKVAPNI
                     LQRCFHSEKPNEKWVTDVTEFDVGGEKIYLSPIMDLFNGEIVSYRIQTRPTFDLAGEI
                     LKGAPEKPGPSEKPMLHSDQGWQYQMFFIKSS"
     gene            complement(837..1127)
                     /locus_tag="ACSIGJ_002122"
     CDS             complement(837..1127)
                     /locus_tag="ACSIGJ_002122"
                     /inference="COORDINATES: protein motif:HMM:NF024908.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHQ1330896.1"
                     /translation="MSQKAAADQLNLPDCSVLPQWLRLYRLNGINGLKPKPKGRKPVK
                     KQYPPQTKKADYLKTKEELFAELAYLKAEAAVLKKLDALKEVRQKERNSSQG"
     gene            1162..>1358
                     /locus_tag="ACSIGJ_002123"
     CDS             1162..>1358
                     /locus_tag="ACSIGJ_002123"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330897.1"
                     /translation="MYSTEYFVVCFLRLMPLSPCNLYLATHLRTNESEMEKYSELTKI
                     RTRRRAAGSTNGTEPIRPALH"
BASE COUNT          301 a          348 c          313 g          396 t
ORIGIN      
        1 ggacagttta atcaagcggc tttcagggac tgaattctgt actgaacagg gctcagtcct
       61 tttaatttca acttgattct atcgttgttg tagtaacgga tatattcgtg cagtgccgct
      121 tccgattcgg taacggaatc atatttgcac gtatggaaac attccgattt caacgttccg
      181 aagaaacttt ccattgccgc attgtccaag cagtttccct tgcgggacag actctgaacc
      241 agaccgttgc ctttcaactg cttttgataa aaaacatctg atattgccaa ccttgatccg
      301 aatgcagcat cggcttttca gacggccccg gtttctccgg cgcacctttc agtatctcgc
      361 cggccaaatc gaaagtcggg cgggtttgaa tacggtaact gacgatttcc ccgttaaaca
      421 aatccataat cggagaaagg tatatctttt ctccgcctac atcgaactcg gtaacgtccg
      481 ttacccattt ctcattcggc ttttctgaat ggaaacagcg ttgcaggatg ttcggcgcaa
      541 ctttgccgac ttctcctttg aacgagcggt atttgcgccg ccgtatcact gccttcagcc
      601 ccgtcttcgc catcagacgg ctgactttct tgtgattgac cggtattcct gcgtgacgga
      661 tggctgccgc aatcctccgg tagccgtatc ttcccttata tcgttgataa atatcatgga
      721 tatgtcgttt caaatcggca tatttgcctt ctgccgattg gacggccaat tggtaataga
      781 aggtgctgcg tggcagtccg acaatcccca acagcagttc aacggatggc atttccttaa
      841 ccctgcgacg agttgcgttc tttctgccgc acttctttca aggcatcgag cttttttagg
      901 acagccgctt ccgctttaag gtaagccaat tccgcaaaca gttcttcctt ggttttcaga
      961 tagtcggctt ttttcgtttg cggcggatac tgttttttca cgggctttct tcctttgggt
     1021 ttgggcttta aaccgttaat accattcaaa cggtagaggc gcaaccattg cggcaaaacg
     1081 gagcagtcgg gcagattcag ttggtctgcg gcagcttttt gggacattcc ctgccccgtc
     1141 accgggcgga ttgcctcaag tttgtattcg accgaatatt ttgtcgtatg ctttctgcgt
     1201 ttgatgccgc tttcaccgtg taatctgtat cttgccaccc atttgcgtac caatgaatcg
     1261 gaaatggaaa aatatagtga attaacaaaa atcaggacaa ggcggcgggc cgcaggcagt
     1321 acaaatggta cggaaccgat ccgcccggcg ctccatca
//
LOCUS       DBJGGI010000138         1306 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.138, whole genome shotgun sequence.
ACCESSION   DBJGGI010000138 DBJGGI010000000
VERSION     DBJGGI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.138"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(1..423)
                     /locus_tag="ACSIGJ_002124"
                     /pseudo
     CDS             complement(1..423)
                     /locus_tag="ACSIGJ_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            608..1168
                     /locus_tag="ACSIGJ_002125"
     CDS             608..1168
                     /locus_tag="ACSIGJ_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HHQ1330898.1"
                     /translation="MKKIIFAALAAAAVGTASATYKVDEYHANVRFAIDHFNTSTNVG
                     GFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQPFTGHLKSADIFDAAQYPDIRFVS
                     TKFNFNGKKLVSVDGNLTMRGKTAPVKLKAEKFNCYQSPMAETEVCGGDFSTTIDRTK
                     WGVDYLVNAGMTKNVRIDIQIEAAKQ"
BASE COUNT          331 a          419 c          301 g          255 t
ORIGIN      
        1 ctgcccgggc tatcggcata aacaataccg tccggcatga ttttcttttt gacggcaggg
       61 ggcaacgttt caggctcggc atcgtccgcg gcaacggtat aggcccgccc gttgcgtttc
      121 ggaatgccga agacaaccgc ttttcctgcc gcgccgcgac cgcgtctgcc tttacgccgt
      181 ccgccgaaac agcttccgcc cggcccggcg gggcccccga aaacctcatc ggcagccaag
      241 gccaaacgat ggttggcaac cgtgcggatt ttacggtaaa acagtgccgc cgaatcggga
      301 tggataccca aaatatcggc ggcagaacgg gcggtaactt ccggtacaaa agaacggagc
      361 ggttcttttt gtacttcttt ctttaatttg cagtgtgtta tcttcatatt tcgagggtaa
      421 catatctgct aatctggtac agacccaaaa acaaagcacg gataacaccg cgctttttca
      481 tccgccctgt tccgcccgcc cgttcgtcaa ccgcctgcaa acccaccgtt taccggcagg
      541 aaatgccata aatccccgcc tatgttagga taaacgcctt taccggccca cacaaaggat
      601 tatcatcatg aaaaaaatca tcttcgccgc gctcgcagcg gcagccgtcg gcactgcctc
      661 cgccacctac aaagtggacg aatatcacgc caacgtccgt ttcgccatcg accacttcaa
      721 caccagcacc aacgtcggcg gtttttacgg tctgaccggt tccgtcgagt tcgatcaagc
      781 aaaacgcgac ggcaaaatcg acatcaccat tcccgtcgcc aacctgcaaa gcggttcgca
      841 acccttcacc ggccacctga aatccgccga catcttcgat gccgctcaat atccggacat
      901 ccgcttcgtt tccaccaaat tcaacttcaa cggcaaaaaa cttgtttccg ttgacggcaa
      961 cctgaccatg cgcggcaaaa ccgcccccgt caaactcaaa gccgaaaaat tcaactgcta
     1021 ccaaagcccg atggcggaaa ccgaagtttg cggcggcgac ttcagcacca ccatcgaccg
     1081 caccaaatgg ggcgtggact acctcgttaa cgccggtatg accaaaaacg tccgcatcga
     1141 catccaaatc gaagctgcaa aacaataagc cttgatgcga atcagcacaa atgccgtctt
     1201 catcaaaccg ccctgccaac cgtaagccga tatggcggag cacaacccgc cgaaagatat
     1261 tttggcggca ggcggggctt gccggaaagt ccggaaagta acgacc
//
LOCUS       DBJGGI010000139         1276 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.139, whole genome shotgun sequence.
ACCESSION   DBJGGI010000139 DBJGGI010000000
VERSION     DBJGGI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.139"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            218..364
                     /locus_tag="ACSIGJ_002126"
     CDS             218..364
                     /locus_tag="ACSIGJ_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330899.1"
                     /translation="MSRIYLPLLFPPHIVERGLLYFQQDKVRDVQKISLGRYRAKVFD
                     SENY"
     gene            complement(741..1190)
                     /locus_tag="ACSIGJ_002127"
     CDS             complement(741..1190)
                     /locus_tag="ACSIGJ_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HHQ1330900.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT          400 a          310 c          191 g          375 t
ORIGIN      
        1 tgagatttta ttaaaattca tgcatctaaa taagcagaac ttaatttcct ccaaacgatc
       61 ggatatcttc aaatgcacat ttcacagaaa ggtcgtctga aacccatttt cagacgacct
      121 ttttccactt cgcaaaacaa gaattcccac ccctctcccc aaacatcgcc caaaccagta
      181 aattccccat atttcaacaa ctccttggaa gcaaaccatg tcccgtatct acctacccct
      241 cctatttccg ccccacatcg tcgaacgcgg ccttttgtat tttcagcagg acaaggttcg
      301 cgatgtccaa aaaatttcct tagggcgtta tcgggcgaag gtattcgatt cggaaaacta
      361 ttaggcatgg ttgaagctgg atagtgattt gtacattaag gacggggctg cgattgccct
      421 tatgtctaaa ggtgcaaaca tgccttaaat tactaagcct ataacgaatt ggaaacaaaa
      481 tgccgtctga aaacatcttc agacggcatt ataaaatctg ttcacctttt cagatgagta
      541 atgtacaccc ctatacaatt tttgctacca tgcaccataa atccacggct aaagataatt
      601 gggtttattc ttttatccag aaacaatcca gatacggatc acgggtcata actataggct
      661 taatattaca cgattctcat tccatcaagg cggaaaaccg cacaaatact gaaacactat
      721 cgatcgattt gtaaacaagc ctacttaagt aacttgcagt ccttatcatt tcctttaaaa
      781 taatccagcc cgtcactaca cgaactggcg gacttcttgc aaataaaggt tactagattt
      841 tcattcatct taataataaa aggattttta tctttatcta tggctaccgc cttcaacatg
      901 aatttactgt ctaaagcccc gcgcgcgatt ccattcaaac ggatacaaaa gccttctgcc
      961 tctttaatcg gcaaacttgg ccatttggta gatgtttgtt taaatctccc attctgcaga
     1021 taaaactttt ccataaaatg tgcattttct aacaaggctg cccgcactgc atttatcttt
     1081 gctttctcaa cataattgcg atagctcgga taaacaatta aagcaagtac agacaatatc
     1141 aagaccactg atattaattc aaccagcgta aacccccgat tatcagtcat tactttactt
     1201 ccaataagaa cagattattc aacatatttc tttgaacaga cttactatcc cattcagcag
     1261 tatgcatatt tcccac
//
LOCUS       DBJGGI010000140         1160 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.140, whole genome shotgun sequence.
ACCESSION   DBJGGI010000140 DBJGGI010000000
VERSION     DBJGGI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.140"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            356..985
                     /locus_tag="ACSIGJ_002128"
     CDS             356..985
                     /locus_tag="ACSIGJ_002128"
                     /EC_number="1.14.14.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004392 - heme oxygenase (decyclizing)
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006788 - heme oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HHQ1330901.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDPELNKAISELEYMARYDAVTQDLKDLGEEPYKFDKELPHE
                     TGNKAVGWLYCAEGSNLGAAFLFKHAQKLDYTGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAQEAFAFYKVILRETFGLPEGTEAPEGMMPHRH"
     gene            complement(1010..>1160)
                     /locus_tag="ACSIGJ_002129"
     CDS             complement(1010..>1160)
                     /locus_tag="ACSIGJ_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HHQ1330902.1"
                     /translation="FWNQAKRALRKYNGIDRKPFPPLLRECEFRLNSGTPPRQLKILR
                     DRCGI"
BASE COUNT          338 a          287 c          272 g          263 t
ORIGIN      
        1 ggcaaacggc ttcatcggga aagtggcaaa tataaatggc ggcattataa aggaaaaaaa
       61 gcgatacacc caatcaggat gcaaaaccgg caggttcggc agtatgaaca aacccttggt
      121 gtccggcaaa aatatttttt gagcggctgc ccctccggcc gacaagccta tttgataaaa
      181 ccccgatttt gtaaataatc ataaaatacc cgcttaaaaa tataacgcaa atgatatttt
      241 ttcgtaaaat atgtgataat aaatttcgtt tatgattctt tattattttt gtatgcgggc
      301 aagtccggat tttgcccgca ccgcatcctt atcccttacc gacaaaggag aacttatgag
      361 cgaaaccgaa aaccaagccc tgacgtttgc caaacgcctg aaggcggata ccacggcggt
      421 tcacgacagc gtggataacc tcgttatgtc tgtccaaccg tttgtcagca aagaaaacta
      481 catcaaattt ttgaaactgc aatccgtgtt ccacaaagcg gttgatcata tctataaaga
      541 ccccgagttg aacaaagcca tttccgagct ggaatacatg gcgcgatacg atgccgtaac
      601 gcaagacttg aaagacttgg gcgaagagcc ttacaaattc gacaaagaat tgccacacga
      661 aaccggcaac aaagccgtcg gctggcttta ttgcgccgaa ggatccaatt tgggcgcggc
      721 atttttgttc aaacacgcgc aaaagctgga ttacaccggc gaacacggcg cgcgccacct
      781 cgctccgcat ccggacgggc gcggcaaaca ctggcgcgcc ttcgtcgagc acctgaatgc
      841 cctgaactta actccggaag cagaagcaga agccattcaa ggcgcgcagg aagcgtttgc
      901 tttctataaa gtgattctgc gtgaaacctt cggtttgccg gaaggaacag aagcccccga
      961 aggcatgatg ccgcacagac attaaaaatg gggctgtact agattatccc taaattccac
     1021 accgatcccg caggattttt agctgccggg gcggtgtgcc ggagttaagt cgaaattcgc
     1081 attccctcaa caacggcggg aaaggtttac gatcgattcc gttgtatttt cgcaaggcgc
     1141 gttttgcctg attccaaaag
//
LOCUS       DBJGGI010000141         1114 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.141, whole genome shotgun sequence.
ACCESSION   DBJGGI010000141 DBJGGI010000000
VERSION     DBJGGI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.141"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(<1..266)
                     /locus_tag="ACSIGJ_002130"
     CDS             complement(<1..266)
                     /locus_tag="ACSIGJ_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330903.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFGGKPADEARFAAVLTETCRRAVKK"
     gene            complement(303..566)
                     /locus_tag="ACSIGJ_002131"
     CDS             complement(303..566)
                     /locus_tag="ACSIGJ_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330904.1"
                     /translation="MADILPSDKRRQPPKTKGNPINIIEQETKRTELRKIDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            complement(586..834)
                     /locus_tag="ACSIGJ_002132"
     CDS             complement(586..834)
                     /locus_tag="ACSIGJ_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330905.1"
                     /translation="MHGFRDLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHH
                     YLTADSYRLQERRELAEWYGRRHRQAYEAAAQNTCNKT"
     gene            complement(795..>1114)
                     /locus_tag="ACSIGJ_002133"
     CDS             complement(795..>1114)
                     /locus_tag="ACSIGJ_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHQ1330906.1"
                     /translation="ARWQDIDLTARLWHIPAADMKTKHPFTVPLSDWAVEILRELHTQ
                     TGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTPANPNTPCTDSATSSP
                     TSA"
BASE COUNT          169 a          232 c          400 g          313 t
ORIGIN      
        1 atttttttga ctgcccggcg gcaggtttcg gtaaggacgg cggcaaatcg ggcttcgtct
       61 gccggtttgc cgccgaaaaa aataaaatcg taaagggtga tgccgttttc ggaatggcgg
      121 taatgcccgc ccgggcattc gccgtctgtc tgcctgtgga ttttggcaat caggcgggga
      181 tagcggcaat gggtaatgaa acggcttgcg ccgtcttgcc cgataatcca ttcggggtag
      241 cggttgaata gtgcggctct gttcattttg ttgagggata aagcccctcg cagggcttgt
      301 ggtcaggcaa atttgaatat cagtgccaac acggcggcga tggcggtaac cagaccggtg
      361 gccgccatca tgggatacca gcgggactct tgggctattt ttacggattc ggcgttgatt
      421 ttgtgcgcgt cggcaatgat tttggcgatt tcggcatcta tttttcttaa ctcggtgcgt
      481 tttgtttctt gttcgattat gtttattgga tttcctttcg tttttggcgg ttgccgccgc
      541 ttgtcggatg gcaggatgtc tgccatgtat gtatgttaat accttttagg ttttattgca
      601 agtgttttgg gcggcggctt cgtatgcttg gcggtggcgg cggccgtacc attcggcgag
      661 ttcgcgccgc tcttggaggc ggtagctgtc ggcggtcagg tagtggtgca ggcttgagaa
      721 gccggcgcgt tggagggcgg cgcggctgat gtggccaagg gcgaggtctg ttgtgagggt
      781 gtcttttccg gcggtcaggc tgatgttggt gaagaggtcg cggaatccgt gcatggtgtg
      841 tttggatttg ccggggtgct gccgtcatag cccagtcgtc ggatggcgtt gtgggcgaat
      901 ttgatgctga tgtggtcggg atggggggcg ggtttgcgcc gtgggcggat gcctgggaaa
      961 aggtggatgt tgtcgccggt ctgtgtgtgc agctctcgga gtatttctac tgcccagtcc
     1021 gacagtggga cggtaaaggg gtgtttggtc ttcatgtcgg cggcggggat gtgccataac
     1081 cgggcggtga ggtcgatgtc ttgccagcgg gcgg
//
LOCUS       DBJGGI010000142         1081 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.142, whole genome shotgun sequence.
ACCESSION   DBJGGI010000142 DBJGGI010000000
VERSION     DBJGGI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.142"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            50..394
                     /locus_tag="ACSIGJ_002134"
     CDS             50..394
                     /locus_tag="ACSIGJ_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HHQ1330907.1"
                     /translation="MNLPFRAMVSDLGGTLLTPEHLVGDLTIDTLRVLEQKGVDIILA
                     TGRNHTDMSSILGKIGAERAVMITSNGARVRDLQGNLLYSNSLPEELVLELYKTSYVG
                     NLIQIPRFCRHQ"
     gene            348..1001
                     /locus_tag="ACSIGJ_002135"
                     /pseudo
     CDS             348..1001
                     /locus_tag="ACSIGJ_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
BASE COUNT          333 a          202 c          246 g          300 t
ORIGIN      
        1 cctgtatatt tagtgatgtg ataagtatag tttatttttt gaggttttta tgaatttacc
       61 ttttcgggca atggtgtctg atttaggtgg cacactttta acacctgaac atttagtggg
      121 tgatcttacc attgatactc tgagagtatt agagcaaaaa ggcgtagata ttattttggc
      181 aacaggacgc aatcataccg atatgtcatc cattcttgga aaaatcggtg cggaacgtgc
      241 ggttatgatt acatcaaacg gtgctcgtgt acgggattta cagggtaatt tgctttatag
      301 caatagtttg cctgaagaat tagttctcga gctttacaaa acatcatatg tgggaaatct
      361 tattcagata ccaagatttt gtcgccatca ataaacctca aggcatatcc gtccaccgtt
      421 ccggcggcga agtcagtctg acggcaacac ttgcggcaca gttgggcgta gaaaaggtat
      481 ggttgctgca caggttggac aagcaggccg gaggcatatt gctgtttgcc cttaatcccc
      541 aaagtgccgc tgttcttgcc gcacagtttg ccgaaaggaa aatgaagaaa acctatttgg
      601 ctttgtccga ccgcaaaccg tccaaaaaac aaggatggat aaaaggcggc atggaaaaat
      661 ccaggcgcgg gatgtggaag ctgacgcgca atatggaaaa tattgccgtt acccggtttt
      721 tcagtatccg catcagtgaa aaaatgcggc tgttcatcct tgagccacat acgggtaaga
      781 cacatcagtt gagggtggta atgaagagtc tgggcagctc tatatttggg gacagcctgt
      841 atggaggaac agaatccgaa actatgttcc tgtatgcgtg aaaaatacaa tttacctatc
      901 aacaacaaga aatcgaaatt accgcacctt tgaaaaatgc atggcaggcc gaaaatatct
      961 ctcatgcact ggaagaattt tatatggcaa aagcaggtta atagatataa atcaattaaa
     1021 acaatatgat aaatattttt ataaaatttt ttcaaaaaac ggttgaccgc aaatctcttt
     1081 a
//
LOCUS       DBJGGI010000143         1058 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.143, whole genome shotgun sequence.
ACCESSION   DBJGGI010000143 DBJGGI010000000
VERSION     DBJGGI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.143"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..594
                     /gene="mafB"
                     /locus_tag="ACSIGJ_002136"
     CDS             <1..594
                     /gene="mafB"
                     /locus_tag="ACSIGJ_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HHQ1330908.1"
                     /translation="AGTVWRGKKVELNPAKWDWVKNTGYETTVTRKTLDGQIAGKNKP
                     IKPSSGDYVLPVGRQIIDPTRTSFSQATVSYQKRGANYNYDSLVAAMNEKKSWVGDRV
                     DVVNMPDGAPTSMDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQTWG
                     EAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            591..962
                     /locus_tag="ACSIGJ_002137"
     CDS             591..962
                     /locus_tag="ACSIGJ_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HHQ1330909.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
BASE COUNT          365 a          176 c          225 g          292 t
ORIGIN      
        1 gcaggtacgg tttggcgcgg taaaaaagta gaacttaacc cggccaaatg ggattgggtt
       61 aaaaataccg gctatgaaac aactgttacc cgaaagactt tagatgggca aatagctggc
      121 aagaacaagc ctattaaacc ttcttcagga gattatgtct tgccggtagg cagacaaatc
      181 atagatccta ctcgtacatc tttttcacaa gctacagtta gctatcagaa gcgtggtgca
      241 aattacaact atgattctct tgtagctgca atgaacgaaa aaaaatcatg ggttggcgac
      301 agggttgatg ttgttaatat gcctgatgga gcacctacta gtatggataa cacgcgtatt
      361 atggcagcac gtgaagcagg ggtaaaagtg gaagcgaatg ttcataattt taatgaccga
      421 ttatcatcaa aagagagaat caggtttaag catgatggta ttgagcctca aacttgggga
      481 gaagctatcc agctacgaat tagaaagcaa gaaacacaaa aaggagttcc agaagggtgg
      541 agcaaaagat ttcctaacgg aagtatttat gatgtaaagg tacttaggaa atgataaaac
      601 aaaatagttt tgttccgtat cctgaagcaa tgcttcctaa aggatttaaa tatccgcaaa
      661 gttatttaaa attagctcaa tccactcatg ccattaacta cgatgaacaa tattcttttc
      721 cttggtggtt tgaaaatgca gaaagcaata tatcagaagt aattgacatt tattttgaaa
      781 taactggcat tccaaaccta ttaccttttg ctagaaacca agagtgggct gcctgttttg
      841 atatttcaga taaatcaggt aatcctaaaa ttatagtagt taatttagat aatacaaaat
      901 attacgagac ttttgaaaat tttgatactt ggctaaaaga agctgaaaat gatggttggt
      961 agcagccgta ggtcggattc tcgaatccga cattttccaa cagcggcatt tcggaaacga
     1021 tagacgcgtc aaatattttt gtcggataca agtatcca
//
LOCUS       DBJGGI010000144         1045 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.144, whole genome shotgun sequence.
ACCESSION   DBJGGI010000144 DBJGGI010000000
VERSION     DBJGGI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.144"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(175..348)
                     /locus_tag="ACSIGJ_002138"
     CDS             complement(175..348)
                     /locus_tag="ACSIGJ_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330910.1"
                     /translation="MTTAVKPPADLVRPCPKLPHLEGNTGADVLPWSLQVIGLYKDCR
                     ARHGALVRALGAD"
     gene            complement(332..676)
                     /locus_tag="ACSIGJ_002139"
     CDS             complement(332..676)
                     /locus_tag="ACSIGJ_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330911.1"
                     /translation="MIGALLKNWKPLLILSAIAFFAVSWQLDRAAQYRRGYGAAVSEV
                     SERLKAAAVEHAEHARKSSAAYQAQKAAREEKERVRYVQTLKIIEKPVYRNACFDADG
                     VRELNAAVDDGG"
     gene            complement(677..>1045)
                     /locus_tag="ACSIGJ_002140"
     CDS             complement(677..>1045)
                     /locus_tag="ACSIGJ_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HHQ1330912.1"
                     /translation="VGHCLGKAGRAVIRDWYRAKAWSMSGLTKLEAPAYGCIAVKPRR
                     GGGHVFFVVGKDAEGRILGLGGNQGNMVSIIPFDPADIDGYFWPSKLIGGKPVPSSPA
                     EGRYRLSDVAATAKQGAGEA"
BASE COUNT          205 a          343 c          289 g          208 t
ORIGIN      
        1 cccattattg gtgcgcttaa taattttgta taacgccccc ttcgcgcaaa caaaacggca
       61 aaaatttgcc gcgtccgccc gatatgtaaa aaatcggggg gaatgccgac gcttcccccg
      121 atgcggccgc caacccggaa ccgacggttc ggttgcaaac tttcggttgg caactcaatc
      181 cgcgcccaac gcccgtacca gcgcgccgtg ccgcgccctg cagtccttgt acaagccgat
      241 gacttgcaac gaccacggca gcacgtccgc gcccgtgttc ccttcaaggt gcggcagttt
      301 cgggcagggc cgcaccaaat cggcgggcgg cttaaccgcc gtcgtcaacg gcggcgttga
      361 gttcgcgcac gccgtcagca tcaaaacagg cattgcggta cacaggtttt tcaatgattt
      421 taagcgtttg cacatagcgc accctttctt tttcctcgcg cgccgccttt tgcgcctgat
      481 acgcggcgga cgatttgcgg gcgtgttcgg cgtgttcgac cgcggcggct ttgaggcgtt
      541 ccgaaacttc cgacaccgcc gcaccgtatc cgcgacggta ttgcgccgcc ctgtccagct
      601 gccaagaaac ggcgaagaac gcgattgcgg acaaaataag cagcggcttc caatttttca
      661 gcaaagcccc aatcatttac gcctcgcccg cgccctgttt cgccgtggcg gcaacgtccg
      721 acaaccggta acgcccttcg gcgggggacg aaggcacagg tttgccgcca atcagcttgg
      781 acggccagaa gtagccgtca atgtccgcag ggtcaaacgg gatgatggat accatattgc
      841 cctgattgcc gcccaagccc aagattctgc cttccgcgtc tttgccgaca acgaagaaca
      901 cgtgtccgcc gccccggcgc ggtttgaccg cgatgcagcc gtatgcgggg gcttcgagtt
      961 tcgtcaaacc cgacattgac caggctttgg cgcgatacca gtccctgatg accgcgcgtc
     1021 ccgctttgcc caggcagtgt ccgac
//
LOCUS       DBJGGI010000145         1039 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.145, whole genome shotgun sequence.
ACCESSION   DBJGGI010000145 DBJGGI010000000
VERSION     DBJGGI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.145"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..735
                     /locus_tag="ACSIGJ_002141"
     CDS             <1..735
                     /locus_tag="ACSIGJ_002141"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HHQ1330913.1"
                     /translation="VSLEDFAGFPLNIRHISITTYARLKLGEYIADCDKVLYLDTDVL
                     VRDGLKPLWDTDLGGNWVGACIDLFVERQEGYKQKIGMADGEYYFNAGVLLINLKKWR
                     RHDIFKMSCEWVEQYKDVMQYQDQDILNGLFKGGVCYANSRFNFMPTNYAFMANGFAS
                     RHTDPLYLDRTNTAMPVAVSHYCGSAKPWHRDCTVWGAERFTELAGSLTTVPEEWRGK
                     LAVPPTKRMLQRWRKKLSARFLRKIY"
     gene            795..>1039
                     /locus_tag="ACSIGJ_002142"
     CDS             795..>1039
                     /locus_tag="ACSIGJ_002142"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HHQ1330914.1"
                     /translation="MQPLVSVLICAYNVEKYFAQSLAAVVNQTWRNLDILIVDDGSTD
                     GTPAIARRFQEQDGRIRIISNPRNLGFIASLNIGLDE"
BASE COUNT          255 a          256 c          285 g          243 t
ORIGIN      
        1 gtaagcctcg aagatttcgc cggcttcccc ttaaacatca ggcacatttc cattacgact
       61 tatgcccgcc tgaaattggg cgaatacatt gccgattgcg acaaagtcct gtatctggat
      121 acggacgtat tggtcaggga cggcctgaag cccttatggg ataccgattt gggcggtaac
      181 tgggtcggcg cgtgcatcga tttgtttgtc gaaaggcagg aaggatacaa acaaaaaatc
      241 ggtatggcgg acggagaata ttatttcaat gccggcgtat tgctgatcaa cctgaaaaag
      301 tggcggcggc acgatatttt caaaatgtcc tgcgaatggg tggaacaata caaggacgtg
      361 atgcaatatc aggatcagga cattttgaac gggctgttta aaggcggggt gtgttatgcg
      421 aacagccgtt tcaactttat gccgaccaat tatgccttta tggcgaacgg gtttgcgtcc
      481 cgccataccg acccgcttta cctcgaccgt accaatacgg cgatgcccgt cgccgtcagc
      541 cattattgcg gctcggcaaa gccgtggcac agggactgca ccgtttgggg tgcggaacgt
      601 ttcacagagt tggccggcag cctgacgacc gttcccgaag aatggcgcgg caaacttgcc
      661 gtcccgccga caaagcgtat gcttcaaaga tggcgcaaaa agctgtctgc cagattctta
      721 cgcaagattt attgacgggg caggccgtct gaagccttca gacggcatcg gacgtatcgg
      781 aaaggagaaa cggattgcag cctttagtca gcgtattgat ttgcgcctac aacgtagaaa
      841 aatattttgc ccaatcattg gccgccgtcg tgaatcagac ttggcgcaac ttggatattt
      901 tgattgtcga tgacggctcg acggacggca cgcccgccat tgcccggcgt ttccaagaac
      961 aggacggcag gatcaggata atttccaatc cccgcaattt gggctttatc gcctctttaa
     1021 acatcgggct ggacgaatt
//
LOCUS       DBJGGI010000146          977 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.146, whole genome shotgun sequence.
ACCESSION   DBJGGI010000146 DBJGGI010000000
VERSION     DBJGGI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.146"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..375
                     /locus_tag="ACSIGJ_002143"
     CDS             <1..375
                     /locus_tag="ACSIGJ_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HHQ1330915.1"
                     /translation="YFVKGSQAETEGVWVHNDCPYGKGNQRYKDAPYHGKNDNSVKSR
                     APTNGQAVLDNSVQVKSTSSQRVGVDKTNNEIVVLNQTRIFNDGSAEYHGHVRNWKNL
                     HTDQQNALKKAGLVNSKGKIKK"
     gene            372..707
                     /locus_tag="ACSIGJ_002144"
     CDS             372..707
                     /locus_tag="ACSIGJ_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330916.1"
                     /translation="MMADKEQVEKMIYSENKDCVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLRSKQGNPDFQGFAEDALDDI
                     SFFIFKKRP"
     gene            755..977
                     /locus_tag="ACSIGJ_002145"
                     /pseudo
     CDS             755..977
                     /locus_tag="ACSIGJ_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
BASE COUNT          317 a          177 c          227 g          256 t
ORIGIN      
        1 tacttcgtca agggcagtca ggcggaaacg gaaggggttt gggttcataa tgattgtccg
       61 tatggaaaag gcaaccaacg atataaagat gctccttatc atggtaaaaa tgataattct
      121 gtgaaaagta gagcgccaac aaatgggcaa gctgtccttg ataactctgt tcaagttaaa
      181 tcgacatctt ctcaaagagt tggagttgat aaaaccaata atgaaatcgt tgtattaaac
      241 caaactagaa tttttaatga cggttctgct gagtatcatg ggcatgtaag gaactggaaa
      301 aatttacata ctgatcaaca aaatgcttta aagaaagcag gattggttaa ttcaaaagga
      361 aaaatcaaaa aatgatggct gataaggaac aagtagaaaa gatgatttat tctgaaaata
      421 aagattgtgt tattgatggt atgctcggta tgaccttcag ctcggaggag gatgaaatcc
      481 cttggatttc cgagaaattg acggagttgt cgaagcataa agacttggat attgccagat
      541 tgtcattaac ctgtttcgga catctcgcca ggatgcatga aaatatcggc gattgcgata
      601 aagtgattgc tttattgcgc tcaaaacaag gtaatccgga ttttcaaggt tttgccgaag
      661 atgcgttaga cgatatttcc ttctttatat ttaaaaagcg tccgtaagcg cggattaaac
      721 ccggaaatcc gatgcgtctg aaagtacatt ccggctttca gacggcatcg gtaacagcca
      781 aaccctgatt tccaaccgca tccatccgtt ttattcggac ggcaaatgga ttaaagcgga
      841 agatttgaaa gcgggaagca ggctgtttgc tgaaaacggt gcagagcaga ccgttcaaag
      901 cgttaccgtc aaaccggaac cgctcaaagc ctacaatctg accgtcgccg actggcatac
      961 ttatttcgtc aagggta
//
LOCUS       DBJGGI010000147          939 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.147, whole genome shotgun sequence.
ACCESSION   DBJGGI010000147 DBJGGI010000000
VERSION     DBJGGI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.147"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..326
                     /locus_tag="ACSIGJ_002146"
     CDS             <1..326
                     /locus_tag="ACSIGJ_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HHQ1330917.1"
                     /translation="VASASDIKGKYVKEVKVEKGVVTATMASSNVNKEIQGKKLSLWA
                     KRQDGSVKWFCGQPVTRDDKAGTVTDAAGNDGKIDTKHLPSTCRDAASAGCIETPPTA
                     FYKNT"
     gene            457..837
                     /locus_tag="ACSIGJ_002147"
                     /pseudo
     CDS             457..837
                     /locus_tag="ACSIGJ_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          283 a          285 c          211 g          160 t
ORIGIN      
        1 gcgtggcatc cgcttcagac atcaaaggca aatatgttaa ggaagttaaa gtcgaaaaag
       61 gcgtcgttac cgccacaatg gcttcaagca acgtaaacaa agaaatccaa ggcaaaaaac
      121 tctccctgtg ggccaagcgt caagacggtt cggtaaaatg gttctgcgga cagccggtta
      181 cgcgcgacga caaagccggc accgtcaccg acgccgccgg caacgacggc aaaatcgaca
      241 ccaagcacct gccgtcaacc tgccgcgacg cagcatctgc cggttgcata gaaacaccac
      301 ctacggcttt ctataaaaat acctaaaccg tcattcccac gaacctacat cccgtcattc
      361 ccacgaaagt gggaatccgg aacgcaaaat ctaaagaaac cgttttcccg ataagtttcc
      421 gtgccgacaa ggctggattc ccgcctgcgc gggaatgacg aagccatcct tttggccgaa
      481 ggtcaaaaat cagccgttac cgaatattac ctgaatcacg gcaaatggcc atccgacaat
      541 agtgccgccg gcgtggcatc ctccccctcc gacatcaaag gcaaatatgt taaaagcgtt
      601 acggtcacaa acggcgtcgt taccgccaaa atgctttcaa gcggcgtaaa caaagaaatc
      661 aaaggcaaaa gactctccct gtgggccagg cgtgaagccg gttcggtaaa atggttctgc
      721 ggacagccgg ttcagcgcgc cgccaaagac aaagacgacg tcaaagccga cgacaccgcc
      781 ggcaccaaaa tcgacaccaa gcacctgccg tcaacctgcc gcgataaatc aactgccgtt
      841 tgcacgaaac accacctacg gctttctata aaaataccta aaccgtcatt cccacgaacc
      901 tacatcccgt cattcccacg aaagtgggaa tccggaacg
//
LOCUS       DBJGGI010000148          889 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.148, whole genome shotgun sequence.
ACCESSION   DBJGGI010000148 DBJGGI010000000
VERSION     DBJGGI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.148"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(<1..108)
                     /locus_tag="ACSIGJ_002148"
     CDS             complement(<1..108)
                     /locus_tag="ACSIGJ_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HHQ1330918.1"
                     /translation="MRNTVGLDISKLTFDATAIVGNAEYSAKFDNDSKGL"
     gene            188..417
                     /locus_tag="ACSIGJ_002149"
                     /pseudo
     CDS             188..417
                     /locus_tag="ACSIGJ_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            775..>889
                     /locus_tag="ACSIGJ_002150"
     CDS             775..>889
                     /locus_tag="ACSIGJ_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HHQ1330919.1"
                     /translation="MNTFFRQTAQAMTAKHIGRFPLSELDQVIDWQPIEQYL"
BASE COUNT          264 a          197 c          174 g          254 t
ORIGIN      
        1 taaacctttt gaatcgttgt caaactttgc cgaatattcg gcattgccga caatggccgt
       61 tgcgtcaaaa gtcaacttgg atatgtccaa tcctacggtg ttacgcatgg gattaccctt
      121 atatattgcg tccctaagaa ggggcgatta acaaaaatta atgccctttc ttagggacgc
      181 aatatataag gtttataccg tcgttgtccc taatgcgcaa tcagcgacat tgttgccaat
      241 tatccgaaag aaagttaagc ctgatggcat tgtatacacg gatacctttc gtagttatga
      301 tgtgcttgat gttagtgaat ttagccattt acgtaagttt aacggcattc ccaaagagca
      361 tttggggcta tatttaaaga aatgccaatg gcatttgaaa tagaatctca aatttagatt
      421 ttaagacaat tagttaatgg gaatttggtc tggttatcta gtacaacccc aaaagttttt
      481 atataaaaac agtttggtaa gttctcttcg tgaagtgctt atgttttgcc acaaaagttc
      541 cataatcatg gcggcatcac ccaagctcca tccttccaag aaaatggcat ctttgttgtg
      601 gttgaaaatc gactccatac gattttggac aagcataggg atagatgttc tgaaaaacgg
      661 cagcgcgggc attctccaac caccttccca aatcttataa tacaaacccg aagacctttg
      721 caaaattccc ctaaaatccc ctaaattccc accaaggcat ttaggggatt tcccatgaac
      781 accttcttcc ggcaaaccgc acaagccatg actgccaaac acatcggccg cttcccgcta
      841 tcggagttgg accaggtgat tgattggcag ccgatcgaac aatacctga
//
LOCUS       DBJGGI010000149          851 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.149, whole genome shotgun sequence.
ACCESSION   DBJGGI010000149 DBJGGI010000000
VERSION     DBJGGI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.149"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
BASE COUNT          245 a          162 c          200 g          244 t
ORIGIN      
        1 ttctgttttt ggttttctgt ttttgtgaaa ataacgggat tttagcttgt gggtatttac
       61 cggaaaaaac agaaaccgct ccgccgtcat tcccgcgaaa gcgggaatct agacattcaa
      121 tactaaggca atttatcggg aatgactgaa actcaaaaaa ctggattccc actttcgtgg
      181 gaatgacgcg attagagttt caaaatttat tctaaatagc tgaaactcaa cgcactggat
      241 tcccgcttgc gcgggaatga cgaatttcag gtttctgttt ttggttttct gtttttgtga
      301 aaataacagg attttagctt gtgggtattt accggaaaaa acagaaaccg ctctgccgtc
      361 attcccgcgc aggcgggaat ctagacattc aatgctaagg caatttatcg gaaatgactg
      421 aaactcaaaa aactggattc tcacttttgt gggaatgacg ggatgcaggt tcgtgggaat
      481 gacgggattt gaggtttctg tttttgattt tctgttttcg taggaatgac ggtttagaag
      541 ttacccgaaa cctcaaaaaa accgaaaccg aacggatcgg attaccgctt tcgcgtttca
      601 aagttacggc gttatcggaa aaacagaaaa tcaaagctgt aagaatttat ttaaaacaac
      661 cgaatttcaa cggatcggat tctcgcctgt agggaatgac ggcggaaggt tttttgtctt
      721 ttctgacaga tgtccgcaat ctgaaatccc gaccgtggga acgacggtat agtggattaa
      781 caaaaaccgg tacggcgttg cctcgcttta gctcaaagag aacgattctc taaggtgctg
      841 aagcaccaag t
//
LOCUS       DBJGGI010000150          790 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.150, whole genome shotgun sequence.
ACCESSION   DBJGGI010000150 DBJGGI010000000
VERSION     DBJGGI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.150"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(1..213)
                     /locus_tag="ACSIGJ_002151"
                     /pseudo
     CDS             complement(1..213)
                     /locus_tag="ACSIGJ_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687602.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 family protein"
     gene            complement(361..>790)
                     /locus_tag="ACSIGJ_002152"
     CDS             complement(361..>790)
                     /locus_tag="ACSIGJ_002152"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330920.1"
                     /translation="HPAAFARPSALSDFFRKPNHAIQTPPARPDAWQAAGEDHGRGPY
                     VQADLAYAYEHITRDYPEATGANQGKISTVSDYFKNIRTRSVHPRLALGYDFGGWRVF
                     SAPAVAAGLHQSLQYYRSRHCRRFRQIHQFRRITPIPQKP"
BASE COUNT          158 a          185 c          280 g          167 t
ORIGIN      
        1 gccaacgccg tactggtttt tgttaatcca ctatagtacg gcacgcgcgg gatttcgatg
       61 cccaagtcgg ctgccgtgcc gggccatgtg cgtcgaacgg attgcaccgc ccggcagaag
      121 cgtcatcccc gtgttgtgca tacctatgat gaaacagagc agaaacagtg cgagcgaagg
      181 gaaaaccgcc ccgctgccgc cgatgtccca ttgcagcgcg ggaatgacgg gttttaggat
      241 tacggtgtat cgggacggtt cgggtattct tgacaggatg ggttctcaag atgttgcgta
      301 gtgtcgggct cgcaaggggg tgcatagaga gggttgcagg gcgggcttca gcccgccgca
      361 tcaaggtttt tggggaatag gcgtgattcg gcggaattga tggatttgac ggaatcggcg
      421 gcaatggcgg gatcggtaat attgaaggga ttggtggagg ccggcggcaa cggcgggggc
      481 ggaaaagacg cgccagccgc cgaaatcgta gccgagggcg agtcgggggt ggacggagcg
      541 ggtgcggatg tttttgaaat aatcgcttac cgtgcttatt ttgccttggt ttgcaccggt
      601 tgcttcggga taatcgcggg tgatgtgctc gtaggcgtaa gccagatccg cctgcacata
      661 cgggccgcgc ccatggtctt cacccgccgc ctgccaagca tcagggcgag caggaggggt
      721 ttgtattgca tggttcggct ttcggaaaaa atcggataat gctgaaggcc gcgcgaaagc
      781 ggccggatgt
//
LOCUS       DBJGGI010000151          776 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.151, whole genome shotgun sequence.
ACCESSION   DBJGGI010000151 DBJGGI010000000
VERSION     DBJGGI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.151"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(28..>776)
                     /locus_tag="ACSIGJ_002153"
     CDS             complement(28..>776)
                     /locus_tag="ACSIGJ_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330921.1"
                     /translation="LQDMRYACNQELINFAEHIVKQVQRLGLYCNTDDPANEESVLFA
                     CREASQAVAQWTKDFDDLSPNQRQLVLRPLSNLFAAYEEFLKDAPARLIAEVSAYSLA
                     VRVAKKAMAFLELDGGLISAVGKVVNGADSRAEARRLKMPYAEFTGRILHAANLLYDV
                     GIQADKELSAMYGKPLNPVRPRRISDVRRPMMKMLVADKGGALVRAVKDSEDVIRHCD
                     NGAGFSCFNWTEHFKRTANLISLMHREAAA"
BASE COUNT          144 a          231 c          224 g          177 t
ORIGIN      
        1 gatacacgca ggtccggatg tgcgtcctca tgccgccgct tccctgtgca tgaggctgat
       61 caggtttgcc gtccgtttga aatgttcggt ccagttgaag cagctgaagc cggcgccgtt
      121 gtcgcaatgc cggatgacgt cttccgagtc ttttacggcc cggaccaacg cgccgccttt
      181 gtccgcaaca agcattttca tcatcggccg gcgcacgtcg cttatccgtc gggggcgcac
      241 gggattcagc ggcttgccgt acatcgccga aagctccttg tccgcctgaa tgcccacatc
      301 gtaaagcagg ttcgcggcgt gtaggatccg gcctgtaaat tccgcgtacg gcattttcag
      361 gcggcgggct tccgcgcgcg aatccgcgcc gttgacgact ttgccgaccg ccgaaatcaa
      421 accgccgtca agttctaaaa acgccatggc tttcttggca acccgcacgg ccaatgagta
      481 tgccgatact tcggctatca gccgtgcagg cgcgtctttc agaaattctt cgtacgcggc
      541 gaagagattt gacagcggac gcagcacgag ttggcgctga ttcgggctaa ggtcgtcgaa
      601 atccttagtc cattgcgcga ccgcctgcga tgcttcgcgg caggcaaaca gtacgctttc
      661 ctcgttcgcg gggtcgtccg tgttgcagta caggcccaag cgttgcacct gtttgacgat
      721 atgttcggcg aagttgatta actcctgatt gcaggcgtaa cgcatatcct gcaggg
//
LOCUS       DBJGGI010000152          674 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.152, whole genome shotgun sequence.
ACCESSION   DBJGGI010000152 DBJGGI010000000
VERSION     DBJGGI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.152"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..>674
                     /locus_tag="ACSIGJ_002154"
     CDS             <1..>674
                     /locus_tag="ACSIGJ_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HHQ1330922.1"
                     /translation="KTFSSLLFSSLLFSSAAQAANEDNGRGPYVQADLAYAAERITHN
                     YPEATGADKGKISTVSDYFKNIRTHSVQPRLSLGYDFGNWRIAADYTRYRKWNESNSS
                     TKKVTERINDNNKETKTEHQENGSFHAASSLGLSAIYDFQISDKFKPYIGARVAYGHV
                     RHQVNSVNKETITVTPVASGGPVITGPTSKPAYHESHSIRRLGLGVIAGVGFDITSNL
                     TLDAGYR"
BASE COUNT          188 a          193 c          137 g          156 t
ORIGIN      
        1 aaaaccttct cttctcttct cttctcttct cttctcttct cttccgcagc gcaggcggca
       61 aatgaagaca atggccgcgg cccgtatgtg caggcagatt tagcctacgc cgccgaacgc
      121 attacccaca attatccgga agcaaccggt gcagacaaag gcaaaataag cacagtaagc
      181 gattatttca aaaacatccg tacccactct gttcaacctc gactttctct tggctacgat
      241 ttcggcaact ggcgcatcgc cgcagattac acgcgctaca gaaagtggaa tgaaagcaat
      301 tcttctacta aaaaagttac tgaacgtata aacgacaaca acaaagaaac caaaacagaa
      361 catcaggaaa acggcagctt ccacgccgct tcttctctcg gcttatcagc catttacgat
      421 ttccaaataa gcgataaatt caaaccctat atcggcgcgc gcgtcgccta cggacacgtt
      481 aggcatcaag ttaattcggt caacaaagaa actattactg ttactcctgt ggcgagtggc
      541 ggccctgtta taacagggcc gaccagcaaa cctgcctatc acgaaagcca cagcatccgc
      601 cgcttgggtc ttggtgtcat cgccggtgtc ggtttcgaca tcacgtccaa tctgacttta
      661 gatgctggct accg
//
LOCUS       DBJGGI010000153          656 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.153, whole genome shotgun sequence.
ACCESSION   DBJGGI010000153 DBJGGI010000000
VERSION     DBJGGI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.153"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
BASE COUNT          155 a          175 c          175 g          151 t
ORIGIN      
        1 cattcccgcg caggcgggaa tccagtccgt tcggtttcag tcatttccaa aaattgccgt
       61 agcgttaagt ttctagattc ccactttcgg gggaatgacg cggtgcaggt ttccgtgcgg
      121 acggattcgt cattcccaca cagacaggaa tccggatttg tccgcgcgga aacttatgcg
      181 ccgtcattcc cgcacaggcg ggaattcagg tctgtcggca taggaactta tcggataaaa
      241 cggttgcccc aaccctgcgt tctagattcc cactttcgtg ggaatgacga ttcaggtatt
      301 cctaatagca acccgccaca acccacctac gcccatccta cgcatctgcc atacagccct
      361 gttccggagc gcgcggataa ggtgcgatgc cgccggcggg catcatagtc ctctctccct
      421 gtgggagaga gctagaaagc tgcaaggctt gtattttaga agactaaggg atttgggaaa
      481 gattgtcgaa attcggggag ccctctcccc cacgggggag ggggcaggtt gcggcagatt
      541 tggcggttgc aggcggtttg aaaaacagat tggatttaca accgttgatt tcaggtcgtc
      601 tgaaaagcaa aaagcagcct gcacaacctg attagtttac aaaaccctta acccca
//
LOCUS       DBJGGI010000154          624 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.154, whole genome shotgun sequence.
ACCESSION   DBJGGI010000154 DBJGGI010000000
VERSION     DBJGGI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.154"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(<1..338)
                     /locus_tag="ACSIGJ_002155"
     CDS             complement(<1..338)
                     /locus_tag="ACSIGJ_002155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330923.1"
                     /translation="MAGGVYLGGKIISPIYHSSQEFSGEPIIYAETNIILCPAEKFLT
                     LKRVFHNGNIFNMNQIITFLSNKQGGVRFDKNYDKYKTWQVAIEKAANFLKLGNPYNE
                     DKLSLSEEHDT"
     gene            complement(432..>624)
                     /locus_tag="ACSIGJ_002156"
     CDS             complement(432..>624)
                     /locus_tag="ACSIGJ_002156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330924.1"
                     /translation="RTDSGELIGFEFNNADNTVTGNADITVKPADPSQHSRPKGLIIR
                     SDRNEPNKNQEVRLNNIAD"
BASE COUNT          169 a          116 c          115 g          224 t
ORIGIN      
        1 gtgtcatgtt cttcactaag agataattta tcttcattat atggattacc gagttttaaa
       61 aaatttgctg ctttttctat agcaacttgc caagttttat atttatcgta atttttatca
      121 aaacgtactc ccccttgttt attggataaa aaagtaatta tctgattcat attaaaaata
      181 tttccattat ggaaaactcg ttttaaagtt aaaaattttt cagcaggaca taaaattata
      241 tttgtttcag catagattat tggttcacca gaaaattctt gtgatgaatg atatataggg
      301 ctaataattt ttccgcctaa ataaactcca ccagccatat agagtggatt aaatttaaac
      361 cggtacggcg ttgcctcgcc ttgccgtact atttgtactg tctgcggctc gccgccttgt
      421 cctgattttt gttaatccgc tatattattt aagcgaactt cttggttttt gtttggttcg
      481 ttacggtcgc tccggataat cagccctttc gggcgcgaat gctgtgaagg gtcggcaggt
      541 ttgaccgtaa tatccgcatt gccggttacg gtattgtccg cattattaaa ctcaaaaccg
      601 atgagttcgc cggaatcggt acga
//
LOCUS       DBJGGI010000155          610 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.155, whole genome shotgun sequence.
ACCESSION   DBJGGI010000155 DBJGGI010000000
VERSION     DBJGGI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.155"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..453
                     /locus_tag="ACSIGJ_002157"
     CDS             <1..453
                     /locus_tag="ACSIGJ_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HHQ1330925.1"
                     /translation="KWPADNGAAGVASPATDIKGKYVKEVKVENGVVTAQMASTGVNN
                     EIKGKKLSLWAKRQAGSVKWFCGQPVKRDAKAGTDDVAKDDAAGNAIETKHLPSTCRD
                     ESSLPGIARITANGRKTTLLPAWHPPPPTSKANMLKRLKLKTASLPPQ"
     gene            372..>610
                     /locus_tag="ACSIGJ_002158"
     CDS             372..>610
                     /locus_tag="ACSIGJ_002158"
                     /inference="COORDINATES: protein motif:HMM:NF012342.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HHQ1330926.1"
                     /translation="MASSPTDIKGKYVKEVEVKNGVVTATMKSDGVNKEIKNKKLSLW
                     AKRENGSVKWFCGQPVTRDNKANDTVADAADKDKI"
BASE COUNT          187 a          181 c          154 g           88 t
ORIGIN      
        1 aaatggcccg ccgacaacgg cgctgccggc gtggcatccc ccgccaccga catcaaaggc
       61 aaatatgtta aggaagttaa agtcgaaaac ggcgtcgtca ccgcccaaat ggcttcaacc
      121 ggcgtaaaca atgaaatcaa aggcaaaaaa ctctccctgt gggccaagcg tcaagccggt
      181 tcggtaaaat ggttctgcgg acagccggtt aagcgcgacg ccaaagccgg caccgacgac
      241 gtcgccaaag acgacgccgc cggcaacgcc atcgaaacca agcacctgcc gtcaacctgc
      301 cgcgatgaat catcgttgcc gggtattgcc cgaatcacgg cgaatggccg gaaaacaacg
      361 cttctgccgg cgtggcatcc tcccccaccg acatcaaagg caaatatgtt aaagaggttg
      421 aagttaaaaa cggcgtcgtt accgccacaa tgaaatcaga cggcgtaaac aaagaaatca
      481 aaaacaaaaa actctccctg tgggccaagc gtgaaaacgg ttcggtaaaa tggttctgcg
      541 gacagccggt tacgcgcgac aacaaagcca acgacaccgt tgccgacgcc gccgacaaag
      601 acaaaatcga
//
LOCUS       DBJGGI010000156          598 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.156, whole genome shotgun sequence.
ACCESSION   DBJGGI010000156 DBJGGI010000000
VERSION     DBJGGI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.156"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            66..>598
                     /locus_tag="ACSIGJ_002159"
     CDS             66..>598
                     /locus_tag="ACSIGJ_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HHQ1330927.1"
                     /translation="MIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTRYLRDHRGRPAY
                     PLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYSTLCRYRNWLAQ
                     DNTLSELLELINRQLTEKGLKIEKASAAVVDATIIQTADSTQRQAIEVDEEGQISGQT
                     TPSKDKDARWIKKNGLY"
BASE COUNT          170 a          190 c          125 g          113 t
ORIGIN      
        1 aaattcccac caagacattt aggggatttc tcaagagcac cttcttccag caaaccgccc
       61 aagccatgat tgccaaacac atcgaccgtt tcccgctatt gaagttggac caggtgattg
      121 attggcagcc gatcgagcag tacctgaacc gtcaaagaac ccgttacctc cgagaccacc
      181 gcggccgtcc cgcctatccc ctgttgtcca tgttcaaagc cgtcctgctc ggacaatggc
      241 acagcctctc cgatcccgaa ctcgaacaca gcctcatcac ccgcatcgat ttcaacctgt
      301 tttgccgttt tgacgaactg agcatccccg attacagcac cttatgccgc taccgcaact
      361 ggctggctca agacaatacc ctgtctgaac tgttggaact gatcaaccgc caactgaccg
      421 aaaaaggcct aaaaatagag aaagcatccg ccgccgtcgt tgacgccacc attattcaga
      481 ccgccgacag cacacagcgt caggccatag aagttgacga agaaggacaa atcagcggcc
      541 aaaccacacc gagtaaggac aaagatgccc gttggataaa gaaaaacggc ctctacaa
//
LOCUS       DBJGGI010000157          594 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.157, whole genome shotgun sequence.
ACCESSION   DBJGGI010000157 DBJGGI010000000
VERSION     DBJGGI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.157"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..591
                     /locus_tag="ACSIGJ_002160"
     CDS             <1..591
                     /locus_tag="ACSIGJ_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HHQ1330928.1"
                     /translation="ANKGKISTVSDYFRNIRTHSIHPRVSVGYDFGGWRIAADYARYR
                     KWNNSKYSVSIKELKNNNKKKTENQENGSFHAVSSLGLSAVYDFKLNDKFKPYIGARV
                     AYGHVRHSIDSTKKITAGAGGAGSPVRPSYKSTQDAHHQSNSIRRVGLGVIAGVGFDI
                     TPKLTLDTGYRYHNWGRLENTRFKTHEASLGVRYRF"
BASE COUNT          171 a          169 c          139 g          115 t
ORIGIN      
        1 gcaaacaaag gcaaaataag cacggtaagc gattatttca gaaacatccg tacgcattcc
       61 atccacccca gggtgtcggt cggctacgac ttcggcggct ggaggatagc ggcagattat
      121 gcccgttaca gaaaatggaa caacagtaaa tattccgtca gcataaaaga gttgaaaaat
      181 aataataaga agaagacgga aaatcaggaa aacggcagct tccacgccgt ttcttctctc
      241 ggcttatcag ccgtttacga tttcaaactc aacgataaat tcaaacccta tatcggcgcg
      301 cgcgtcgcct acggacacgt cagacacagc atcgattcga ctaaaaaaat aacagccggt
      361 gctggtggcg cagggtctcc ggttcgtcct tcttacaaaa gtacgcaaga cgcccatcac
      421 caaagcaaca gcatccgccg cgtgggtctc ggcgtcatcg ccggcgtcgg tttcgacatc
      481 acgcccaagc tgaccctgga caccgggtac cgctaccaca actggggacg cttggaaaac
      541 acccgcttca aaacccacga agcctcattg ggcgtgcgct accgcttctg attc
//
LOCUS       DBJGGI010000158          591 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.158, whole genome shotgun sequence.
ACCESSION   DBJGGI010000158 DBJGGI010000000
VERSION     DBJGGI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.158"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            281..>591
                     /locus_tag="ACSIGJ_002161"
     rRNA            281..>591
                     /locus_tag="ACSIGJ_002161"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          122 c          179 g          136 t
ORIGIN      
        1 ttgactgttt tcccctgcgg gcgtatagtt cggttcttcg ccgctgccga agcggcggac
       61 gaactgaaaa gtatagcacg gaatgtcgga aatatcgaga gatatcttag acaggcggaa
      121 gggatacttt ataatttcgc aacgctcttt aacaaaacag attaccgata agtgtgagtg
      181 ccttgggcct cgcactgttt gaaagacaga cgagatgatg ttttgaacat tgtcctgtcg
      241 gtttctttga agcagaccag aagttaaaaa gttagagatt gaacataaga gtttgatcct
      301 ggctcagatt gaacgctggc ggcatgcttt acacatgcaa gtcggacggc agcacaggga
      361 agcttgcttc tcgggtggcg agtggcgaac gggtgagtaa catatcggaa cgtaccgggt
      421 agcgggggat aactgatcga aagatcagct aataccgcat acgtcttgag agggaaagca
      481 ggggaccttc gggccttgcg ctatccgagc ggccgatatc tgattagctg gttggcgggg
      541 taaaggccca ccaaggcgac gatcagtagc gggtctgaga ggatgatccg c
//
LOCUS       DBJGGI010000159          590 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.159, whole genome shotgun sequence.
ACCESSION   DBJGGI010000159 DBJGGI010000000
VERSION     DBJGGI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.159"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            complement(483..>590)
                     /locus_tag="ACSIGJ_002162"
     CDS             complement(483..>590)
                     /locus_tag="ACSIGJ_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HHQ1330929.1"
                     /translation="PKLTLDTGYRYHNWGRLENTRFKTHEVSLGVRYRF"
BASE COUNT          124 a          139 c          183 g          144 t
ORIGIN      
        1 cggtattgtc gggaatgacg gatttcaaga ttacggtgtt gtcggacggg tttcgagatt
       61 acggtgttgt cggaacgcaa ctgaaccgtc attcccacga aagtgggaat ctagaatctc
      121 gggggttcag tcatttccga tagattcccg ccgcgtcagg gggtctggat tcccgcctgc
      181 gcgggaatga cgaatccatc catacggaaa cctgcaccac gtcattccca cgaaagtggg
      241 aatctagaat ctcgggattt cagtcatttc cgatagattc ccgccgcgtc ggaggtctgg
      301 attcccgcct gcgcgggaat gacgaatttc aagattgcgg tattgtcggg aatgacgggt
      361 ttcgagatta cggtgttgtc gggaatgacg gttggggtat ttccacgccc gccccgcgtc
      421 tgtaaacggc aggtgcatca aaaatgccgt ctgaaggttc agacggcatc ggtatcgggg
      481 aatcagaagc ggtagcgcac gcccaatgag acttcgtggg ttttgaatcg ggtgttttcc
      541 aagcgtcccc agttgtggta gcggtatccg gtgtccaagg tcagcttggg
//
LOCUS       DBJGGI010000160          572 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.160, whole genome shotgun sequence.
ACCESSION   DBJGGI010000160 DBJGGI010000000
VERSION     DBJGGI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.160"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            248..>572
                     /locus_tag="ACSIGJ_002163"
     CDS             248..>572
                     /locus_tag="ACSIGJ_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330930.1"
                     /translation="MTKLYAEIAEMEAQDDGTVKVWGYASSEAVDSDGEVIAAAAMKA
                     AIPDYMKFGEVREMHGSNAAGTAIEINVEDDGRTFFGAHIVDPVAVEKVKTGVYKGFS
                     IGGSVT"
BASE COUNT          165 a          128 c          162 g          117 t
ORIGIN      
        1 aaaataggga gggatgtcgt gatatttgaa aaatcaataa acggctggaa ataccgcgcc
       61 gtaatggaaa tcagaaccac gcaaaaggag cttgccttga acagccttta tacaacagac
      121 aggattgaat agtcatgaca aaacctgcga cgagtaactg tcaatctttc gattccgtcc
      181 tcatacatcc taagcgtctt tgcaaattct gttcagttat accatttata cagacaggag
      241 caaaccaatg acgaagttat acgcagaaat cgccgaaatg gaggcgcagg acgacggcac
      301 ggtcaaagtt tggggttatg cctcaagcga ggcggtcgat tcggacggcg aagtcatcgc
      361 ggcggcggct atgaaggcgg cgattcccga ttatatgaag ttcggcgagg tgcgcgagat
      421 gcacggcagc aacgcggcgg gaacggcgat tgaaatcaac gtggaagacg acggcagaac
      481 ctttttcggg gcgcatatcg tcgatcccgt tgcagtggag aaagtcaaaa cgggcgttta
      541 caagggcttt tccatcggcg gcagcgttac cg
//
LOCUS       DBJGGI010000161          494 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.161, whole genome shotgun sequence.
ACCESSION   DBJGGI010000161 DBJGGI010000000
VERSION     DBJGGI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.161"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..>494
                     /gene="mafA"
                     /locus_tag="ACSIGJ_002164"
     CDS             <1..>494
                     /gene="mafA"
                     /locus_tag="ACSIGJ_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HHQ1330931.1"
                     /translation="KAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPDSATRYSY
                     PAYDTTATTKSDALSGVTTSTSVLNAPAAALTKNNGRKGERSAGLSVNGTGDYRNETL
                     LANPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAET
                     LKAQT"
BASE COUNT          120 a          198 c          104 g           72 t
ORIGIN      
        1 aaagccgccc tttacgtctc cgttatgggc gaccaaggtt cgggcaacat aagcggcgga
       61 cgctactcca tcgacgcact gatacgcggc ggctaccaca acaaccccga cagcgccacc
      121 cgatacagct accccgccta tgacactacc gccaccacca aatccgacgc gctctccggc
      181 gtaaccactt ccacatcggt cttaaacgcc cccgccgccg ccctgacgaa aaacaacgga
      241 cgcaaaggcg aacgctccgc cggactgtcc gtcaacggca cgggcgacta ccgcaacgaa
      301 accctgctcg ccaacccccg cgacgtttcc ttcctgacca acctcatcca aaccgtcttc
      361 tacctgcgcg gcatcgaagt cgtaccgccc gaatacgccg acaccgacgt attcgtaacc
      421 gtcgacgtat tcggcaccgt ccgcagccgt accgaactgc acctctacaa cgccgaaacc
      481 cttaaagccc aaac
//
LOCUS       DBJGGI010000162          474 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.162, whole genome shotgun sequence.
ACCESSION   DBJGGI010000162 DBJGGI010000000
VERSION     DBJGGI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.162"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
BASE COUNT          122 a          156 c          105 g           91 t
ORIGIN      
        1 agattggcgg gatgaagcct accctgtagc ccgcctttta tagcggatta acaaaaaccg
       61 gtacgggttg ccccgccccg gctcaaaggg aacggttccc taaggcgccc aagcaccggg
      121 cgaaccggtt ccgtaccatt cgtactgcct gcggcccgcc gccttgcccc gatttttgtt
      181 aatccgctat acgaacccgc cccctcccga aaaacgcaaa aaatgccgtc cgaaaacctt
      241 tcggacggca ttttcgcgtg aaccgtcatt cccacgaaaa cagaaaacca aaatcagaaa
      301 cctaaaattc gtcattccca cgaaagtggg aatccaggac tcaggacctg agaaaccttt
      361 ttacccgata agttcccgta ccgaccggtc cggattcccg cctgcgcggg aatgacgcgt
      421 ttcatttgcc gccccctccc gaaaaacgca aaaaatgccg tccgaagacc tttc
//
LOCUS       DBJGGI010000163          448 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.163, whole genome shotgun sequence.
ACCESSION   DBJGGI010000163 DBJGGI010000000
VERSION     DBJGGI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.163"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..411
                     /locus_tag="ACSIGJ_002165"
     CDS             <1..411
                     /locus_tag="ACSIGJ_002165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHQ1330932.1"
                     /translation="PRASFRSHPFGRRSKKGQKSAVAGYCPNHGIWPENFVIPAKAGI
                     QSVQFRSFPINSCCFSFLDSRLRGNDGGAVSVAPDKFLKLKISSFLQGQKTKNRNLKF
                     VIPAKAEIQVCRHGNLSGKKVSPVLSPGFPLSWE"
BASE COUNT          123 a          115 c          103 g          107 t
ORIGIN      
        1 ccgcgcgcaa gtttccgaag ccatcctttt ggccgaaggt caaaaaaagg tcaaaaatca
       61 gccgttgccg ggtattgccc gaatcacggc atatggccgg aaaacttcgt cattcccgcg
      121 aaagcgggaa tccagtccgt tcagtttcgg tcatttccga taaattcctg ttgcttttca
      181 tttctagatt cccgcctgcg cgggaatgac ggcggagcgg tttccgttgc tcccgataaa
      241 ttcctaaaac ttaaaatttc atcattccta caaggacaga aaaccaaaaa cagaaaccta
      301 aaattcgtca ttcccgcgaa agcggaaatc caggtctgtc ggcacggaaa cttatcgggt
      361 aaaaaggttt ctccggtcct gagtcctgga ttcccacttt cgtgggaatg acgggattta
      421 atgatgccgc cggcaacgaa aaaatcga
//
LOCUS       DBJGGI010000164          412 bp    DNA     linear   BCT 14-JUL-2025
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain AUSMDU00099217
            SAMN49748584-rid26816193.denovo.164, whole genome shotgun sequence.
ACCESSION   DBJGGI010000164 DBJGGI010000000
VERSION     DBJGGI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN49748584
            Sequence Read Archive: SRR34364793
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Neisseriales; Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JUL-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002824122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002824122.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/04/2025 03:01:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,166
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 1,918
            CDSs (with protein)               :: 1,918
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 112 of 192
            Pseudo Genes (incomplete)         :: 86 of 192
            Pseudo Genes (internal stop)      :: 41 of 192
            Pseudo Genes (multiple problems)  :: 46 of 192
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN49748584-rid26816193.denovo.164"
                     /strain="AUSMDU00099217"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-18"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1580"
     gene            <1..>412
                     /locus_tag="ACSIGJ_002166"
     CDS             <1..>412
                     /locus_tag="ACSIGJ_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110-like element ISNgo2 family transposase"
                     /protein_id="HHQ1330933.1"
                     /translation="SECAAMKNRHHAAKDEEAAKAYAQIIKAMNEQLEVLKEKIKEQT
                     EKPNCKEGVKRLETIPAIGRMTAAVLFHHLTSSKFETSNKFAAFAGLSPQQKESGTSV
                     RGKGKLTKFGNRKLRAVLFMPAMVAYRIRAFPDFI"
BASE COUNT          144 a           92 c          101 g           75 t
ORIGIN      
        1 aagcgaatgc gcggcaatga aaaaccgtca tcacgcggca aaagatgaag aagcggccaa
       61 agcatatgcg caaatcatca aagccatgaa tgaacagctt gaagttttaa aggagaagat
      121 aaaagagcag acggagaagc ctaactgcaa ggaaggcgtg aagcgtcttg aaaccatacc
      181 ggcaataggc agaatgaccg cagccgtatt gtttcatcat ctaacatctt cgaaatttga
      241 aacatcaaac aaatttgcag cgttcgcagg cttaagcccg caacaaaaag aatccgggac
      301 aagcgtaagg ggaaaaggca aactgaccaa gtttggcaac aggaaattac gcgccgtctt
      361 gtttatgccg gccatggtcg cataccggat aagggcattt cccgacttca tc
//
